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Submission Information

Submission ID : 25349302 Submission Name : Plumbane, tetraethylAR Number : 

Chemical Information

CAS Number : 78-00-2 Ninth Collective Index Name : Plumbane, tetraethylSubmitter Information

Submitter's Name : EPA

Address : 

City : Washington

State : DC

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Additional Technical Point of Contact Information

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Sponsor Information

Sponsor's Name : EPA

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City : 

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Phone Number : 

Fax Number : 
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 Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Composition of test item (w/w): 61.3% tetraethyl lead; 35.68% 1,2-dibromoethane;

0.026% blue dye; 2.93% kerosene and inerts; 0.06% antioxidant AO-29.

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1975

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Pimephales promelas

GLP: No

Analytical

Monitoring:

No Data

Test Type: Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

0, 0.005, 0.0075, 0.01, 0.025, 0.05, 0.075, or 0.1 mg/L

Exposure Period: 96 Hours
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Vehicle Used: Yes

Vehicle Name: Acetone

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value: Value/Lower Range: = 7.8

Test Temperature

and Units:

Value/Lower Range: 19.8 Upper Range: 20.2 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

The test material, as either a 10, 0.1 or 0.01% solution in acetone, was introduced

into all-glass vessels containing ten fathead minnows in 15 liters of water. Fish

were not fed during exposure. No aeration was provided during the test. Dissolved

oxygen and pH were measured at the beginning of exposure. Calculation of the 96-hour

LC50 was via straight line graphical interpolation. An acetone control was included,

and prepared by adding, to clean supply water, a volume of acetone equal to the

greatest volume of test solution used. A negative water control was also included.

Test species: Pimephales promelas (Fathead minnow); 3.7 cm standard length.

Limit Test: No

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.037 mg/L Mortality
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Results Remarks: The test temperature was maintained at 20 ± 0.2 oC. The pH was 7.8. Mortality was

100% at the two highest test concentrations, 90% at the third-highest concentration,

and 0% at all other tested concentrations. Tetraethyl lead exhibits extreme acute

toxicity to fathead minnows.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

No guideline or method was cited, and GLP compliance is unknown. Additionally, it is

unclear whether analytical monitoring was performed, and dissolved oxygen

concentrations and total organic carbon was not specified.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: TSCATS submission (OTS0555568, Doc ID 88-920010086, received 10/27/92): Haskell

Laboratory, DuPont Chemical Company. 1975. Initial submission: Report of 96-hr LC50

of Lead, Tetraethyl in Fathead Minnows with Cover Letter Dated 101592.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

98% pure, supplied by Ellesmere Port Research Laboratories of the Associated Octel

Company Limited 

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1980

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

see below 

Deviations from

Method/Guideline:

Yes

Species: Pleuronectes platessa
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GLP: No Data

Analytical

Monitoring:

Yes

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

Measured

Nominal and

Measured

Concentrations:

0, 0.02 to 0.2 mg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 15 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Test type: Acute Toxicity to Fish - Flow-through

Test species: Pleuronectes platessa (European plaice; saltwater fish). Mean length:

5.2 cm; mean weight: 2.8 g. Source: Tor Bay, S. Devon; obtained via otter trawl. 

Test method/conditions remarks: All fish were maintained in flowing sea-water for a

minimum of 7 days prior to test item exposure. Seawater (salinity 34.9 per mille )

was pumped directly from the sea and supplied at a constant temperature (15 oC) to

the test system. At the design flow rate of 100 mL/min, the 90% replacement time was

7.5 hours. 

Stock solutions were prepared by agitating 22 liters of water with 10 grams of

tetraethyl lead for 4 hours, allowing the excess to settle for 2 hours, and then

pumping the top 20 liters into a sealed stock vessel isolated from direct sunlight. A

concentration of 0.02 to 0.2 mg Pb/L tetraethyl lead was maintained with a variation

of ±10%.

Ten fish were transferred to a test vessel and maintained in flowing seawater for 24

hours, at which time a pre-selected amount of the stock solution was fed continuously

into the vessel for a period of 96 hours. A control vessel was included. Test

vessels were regularly monitored for mortality. Water samples were taken twice daily

to analyze tetraethyl lead content. Temperature, dissolved oxygen, and pH were

measured daily. Test animals were fed prior to but not during exposure.
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Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.23 mg/L Mortality

Results Remarks: The 96-hour LC50 value was 0.23 mg Pb/L.

The LC50 value was calculated according to the methods of Doudoroff et al. (1951).

The study authors reported that the results show that the plaice is more resistant to

the effects of tetraethyl lead than to tetramethyl lead.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

No guideline or method was cited, GLP compliance is unknown, individual data on

mortality were not provided, and the exact tested concentrations were

unclear. Dissolved oxygen, pH, and total organic carbon were not

specified. Additionally, the test was performed with a non-standard marine species.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of some

lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad (eds.), Lead in

the Marine Environment. Proc. Int. Experts Discussion on Lead Occurrence. Fate and

Pollution in the Marine Environment, Rovinj, Yugoslavia: 233-261. Pergamon, Oxford,

UK.
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 Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Purity not specified 

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1964

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Lepomis macrochirus

GLP: No

Analytical

Monitoring:

No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:
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pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

GLP compliant?: The study pre-dates GLP.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.2 mg/L Mortality

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

The IUCLID summary lacked sufficient information to assess data adequacy.
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Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Primary reference (not available): Makina, J.F. 1964. Toxicology of petrochemicals

in the aquatic environment - water and sewage works, vol 3, pp. 456-460. 

Secondary reference: IUCLID Dataset for CAS No. 78-00-2, available at

http://ecb.jrc.ec.europa.eu/esis/

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Purity not provided; test substance described as a commercial product, "TEL-CB" 

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1978

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Yes

Species: Morone sp.

GLP: No

Analytical

Monitoring:

No

Test Type: No Data

Test Vessel: Closed

Water Media Type: Saltwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 48 Hours
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Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 20 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Test species: 6-mm larvae of Morone labrax (European seabass)

The tetraethyl lead solution was prepared by adding 2 ml of product to 1 L of

filtered natural seawater (0.45 μm) and slowly stirring magnetically for 1 hour. The

upper one fourth of the saturated solution was used for preparing experimental

dilutions in natural filtered seawater. Analytical measurement by atomic absorption

spectrophotometry gave a content of 2 mg total lead/L for the stock solution. The

experiment was carried out in the dark, at 20 oC, using closed vessels to avoid gasphase leaching of the volatile alkyl lead compound.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % = 65 ppb Mortality
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Results Remarks: The 48-hr LC50 was 65 ppb Pb. One-hundred percent mortality was seen after 48 hours

at 130 ppb Pb. All larvae died almost immediately at concentrations of 650 ppb

Pb. There were no mortalities at 10 ppb Pb. Alkyl leads show higher toxicity than

inorganic lead.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

The test duration was less than the OECD-recommended length of 96

hours. Additionally, the tested concentrations were unclear; a non-standard fish

species was tested; and missing experimental details included loading rate, pH,

salinity, dissolved oxygen concentration, total organic carbon levels, and whether the

test was conducted in static or flow-through chambers.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Marchetti, R. 1978. Acute toxicity of alkyl leads to some marine organisms. Marine

Pollution Bulletin, 9: 206-207.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Purity not specified 

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1977

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Other
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Other Species: various - see below

GLP: No

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Saltwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 30 Minutes

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

GLP compliant?: The study pre-dates GLP.

Test species: Gobius niger (black goby), Trachurus trachurus (Atlantic horse

mackerel), Mugil cephalus (striped mullet), Atherina boyeri (sand smelt), Trachinus

draco (greater weever) 

Analytical monitoring: Not specified

Exposure duration: 30 minutes

Concentrations tested: Not specified

Test method/conditions remarks: The IUCLID summary of the study was mostly in a

language other than English.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:
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NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

Results Remarks: The IUCLID summary of the study was mostly in a language other than English.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Test duration is less than the OECD-recommended length of 96 hours and tests were

conducted using non-standard fish species. Additionally, the IUCLID summary, which

was mostly in a language other than English, lacked study details. 

The IUCLID summary noted an assigned Klimisch code of 2 for the study.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Primary reference (not available): Giaccio, M. 1977. Quad. Merceol. 16: 55-62. 

Secondary reference: IUCLID Dataset for CAS No. 78-00-2, available at

http://ecb.jrc.ec.europa.eu/esis/

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethyl-
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Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Purity not specified 

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1954

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Lepomis macrochirus

GLP: No

Analytical

Monitoring:

No

Test Type: Static

Test Vessel: No Data

Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 48 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity: 38-81

Dissolved Oxygen: 5 ppm or greater

pH Value: Value/Lower Range: = 6.9 Upper Range: 7.5

Test Temperature

and Units:

Value/Lower Range: 20 °C

Photo

(Light/Dark):

Salinity:
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TOC:

Water Hardness: 84 - 163 ppm

Method/Guideline

Test Conditions

Remarks:

Method/guideline followed: Not specified

Test type: Acute Toxicity to Fish - Static

GLP compliant?: The study pre-dates GLP.

Test species: Bluegill sunfish (Lepomis macrochirus), obtained from the Pennsylvania

State Hatchery (Torresdale, PA) and Hiram People's Hatchery (New Providence,

PA). Average length: 7 cm. Average weight: 5 g. 

Test method/conditions remarks: The test chemical was added to the 5-gallon test jar

at the start of the test period. No subsequent additions were made during the course

of the test. Test jars were filled to the 15-liter mark and placed in a 20 oC

thermostatically-controlled water bath. Carbon-filtered Philadelphia tap water was

used. Fish were acclimatized to the test water and temperature for 10 days prior to

the test period. Ten fish per jar were tested. Based on the average fish weight of

5 g and the number of fish per unit volume of test solution (10 fish/15 L), the

loading rate was 3.3 g fish/L, which exceeds the OECD-recommended maximum loading rate

of 1 g for static tests. Characteristics of dilution water: total alkalinity as CaCO3

= 38-81 ppm; pH = 6.9-7.5; total hardness as CaCO3 = 84-163 ppm; specific

conductivity = 228-301 micromhos; dissolved solids = 154-210 ppm; sulfates as SO4 =

56-98 ppm; chlorides as Cl = 3-26 ppm. Dissolved oxygen concentration was maintained

at 5 ppm or greater throughout the test by gentle aeration. 

Mortality was recorded after 24 and 48 hours. The LC50 was obtained by interpolation

from a graph of the logarithm of the concentration versus the percentage mortality.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % = 1.4 mg/L Mortality

24 Hours LC 50 % = 2 mg/L Mortality
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Results Remarks: The 48-hr LC50 value was 1.4 mg lead/L. The 24-hour LC50 value was 2.0 mg lead/L.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

Test duration is less than the OECD-recommended length of 96 hours; no analytical

monitoring was performed; loading rate exceeded the OECD-recommended limit.

The IUCLID summary noted an assigned Klimisch code of 2 for the study.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Turnbull, H. et al. 1954. Toxicity of various refinery materials to fresh water

fish. Ind. Eng. Chem. 46: 324-333.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Composition of test item (w/w): 61.3% tetraethyl lead; 35.68% 1,2-dibromoethane;

0.026% blue dye; 2.93% kerosene and inerts; 0.06% antioxidant AO-29.

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1976

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Lepomis macrochirus

GLP: No

Analytical

Monitoring:

No Data

Test Type: Static

Test Vessel: No Data
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Water Media Type: Freshwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

0, 0.010, 0.025, 0.050, 0.075, 0.10, 0.25, 0.5 mg/L

Exposure Period: 96 Hours

Vehicle Used: Yes

Vehicle Name: Acetone

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen: 8.6 to 8.7 ppm

pH Value: Value/Lower Range: = 7.7 Upper Range: 8

Test Temperature

and Units:

Value/Lower Range: 19.8 Upper Range: 20.2 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

The test material, as either a 0.1 or 0.01% solution in acetone, was introduced into

all-glass vessels containing ten bluefish in 15 liters of water. Fish were not fed

for 48 hours prior to testing, nor during exposure. No aeration was provided during

the test. Dissolved oxygen and pH were measured at the beginning of

exposure. Calculation of the 96-hour LC50 was via straight line graphical

interpolation. An acetone control was included, and prepared by adding, to clean

supply water, a volume of acetone equal to the greatest volume of test solution

used. A negative water control was also included.

Limit Test: No

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:
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LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.15 mg/L Mortality

Results Remarks: The test temperature was maintained at 20 ± 0.2 oC. The pH measurements across tested

concentrations ranged from 7.7 to 8.0 and dissolved oxygen ranged from 8.6 to 8.7

ppm. Mortality was 100% at 0.25 and 0.50 mg/L, 10% at 0.10 mg/L, and 0% at all other

tested concentrations. Tetraethyl lead exhibits extreme acute toxicity to bluegill

sunfish.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

No guideline or method was cited, and GLP compliance is unknown. Additionally, it is

unclear whether analytical monitoring was performed and total organic carbon was not

specified.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: TSCATS submission (OTS0546521, Doc ID 88-920010136, received 10/27/92): Haskell

Laboratory, DuPont Chemical Company. 1976. Initial submission: 96-hr LC50 to

Bluegill Sunfish with Cover Letter Dated 101592.

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

98% pure, supplied by Ellesmere Port Research Laboratories of the Associated Octel

Company Limited

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Invertebrates

Year Study 1980
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Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Yes

Species/In Vitro

System:

Crangon crangon

GLP: No Data

Analytical

Monitoring:

Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Saltwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

0.01 to 0.2 mg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 15 °C

Photo

(Light/Dark):

Salinity: 34.9

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Test species: Crangon crangon (brown shrimp). Mean length: 4.8 cm; mean weight: 1.1

g. Source: Tor Bay, S. Devon; obtained via push net.

All shrimp were maintained in flowing sea-water for a minimum of 7 days prior to test

item exposure. Seawater (salinity 34.9 per mille ) was pumped directly from the sea

and supplied at a constant temperature (15 oC) to the test system. At the design

flow rate of 100 mL/min, the 90% replacement time was 7.5 hours. 

Stock solutions were prepared by agitating 22 liters of water with 10 grams of

tetraethyl lead for 4 hours, allowing the excess to settle for 2 hours, and then
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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pumping the top 20 liters into a sealed stock vessel isolated from direct sunlight. A

concentration of 0.01 to 0.2 mg Pb/L tetraethyl lead was maintained with a variation

of ±10%.

Ten shrimp were transferred to a test vessel and maintained in flowing seawater for 24

hours, at which time pre-selected amount of the stock solution was fed continuously

into the vessel for a period of 96 hours. A control vessel was included. Test

vessels were regularly monitored for mortality. Water samples were taken twice daily

to analyze tetraethyl lead content. Temperature, dissolved oxygen, and pH were

measured daily. Test animals were fed prior to but not during exposure.

Limit Test:

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.02 mg/L Mortality

Results Remarks: The 96-hour LC50 value was 0.02 mg Pb/L. The LC50 value was calculated according to

the methods of Doudoroff et al. (1951). 

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

No guideline or method was cited, GLP compliance is unknown, individual data on

mortality were not provided, and the exact tested concentrations were

unclear. Additionally, the test employed a non-standard species and dissolved oxygen,

pH, and total organic carbon were not specified.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates
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Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of some

lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad (eds.), Lead in

the Marine Environment. Proc. Int. Experts Discussion on Lead Occurrence. Fate and

Pollution in the Marine Environment, Rovinj, Yugoslavia: 233-261. Pergamon, Oxford,

UK.

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Concentration of tetraethyl lead not specified; lead content: 0.6247 g Pb/mL; clear

red liquid; solvent/carrier: reagent grade absolute ethanol

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

1976

Method/Guideline

Followed:

Other

Other

Method/Guideline:

Methods of Butler et al. (1960) and Butler (1965)

Deviations from

Method/Guideline:

Species/In Vitro

System:

Other aquatic crustacea

GLP: No

Analytical

Monitoring:

Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Saltwater

Test

Concentrations:

Both

Nominal and

Measured

Concentrations:

Nominal: 0 (solvent control), 1.0, 10, 56, 100, 200 μg Pb/L; measured: 0, 1.0, 5.2,

36, 72, 131 μg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:
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Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 28 Upper Range: 30 °C

Photo

(Light/Dark):

Salinity: 30

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Test species: Eastern oyster (Crassostrea virginica), 2-4 cm from umbo to distal valve

edge, wild-caught (Pensacola, FL)

Oysters were held in tanks of flowing, natural seawater of ambient salinity and

temperature for a minimum of two weeks before testing. Oysters were cleaned of

attached organisms and ground by hand on a fine-grit grinding wheel to remove

approximately 2-5 mm of peripheral shell. Six groups of ten oysters each were

exposed to each test concentration for 96 hours. The test was conducted in a glass

system that was covered, where feasible, to reduce volatilization of the test

substance. The system was 18 L, received seawater at a rate of 30 L/hour, and ~99%

replacement occurred every 3 hours. New shell growth was measured to the nearest 0.1

mm using a binocular dissecting scope equipped with a micrometer.

Limit Test:

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours EC 50 % > 1 ug/L Other
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Results Remarks: The 96-hour EC50 value for shell growth was > 1 but < 5.2 μg Pb/L.

The percentage reduction of new shell growth of exposed oysters, as compared to

control oysters was 43, 85, 85, 85, and 100% at concentrations of 1.0, 5.2, 36, 72,

and 131 μg Pb/L, respectively. Salinity was 30-32 per mille and temperature was 28-

30 °C.

Shell growth of oysters exposed to the test substance in seawater was reduced by more

than 50% in concentrations of tetraalkyl lead ≥ 5.2 μg Pb/L during an acute

exposure.

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

The concentration of tetraethyl lead in the test substance was not specified.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: TSCATS submission (OTS0200597, Doc ID 88-7800249, received 12/10/79): Haskell

Laboratory, EI DuPont de Nemours & Company. 1978. Bioaccumulation and Depuration of

Lead by Eastern Oysters (Crassostrea virginica) Exposed to a Tetraethyl Lead Motor

Fuel Antiknock Compound with Attachments and Cover Letter Dated 120379, conducted by

EG&G Bionomics, Marine Research Laboratory, Appendix A. 

 Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

98% pure, supplied by Ellesmere Port Research Laboratories of the Associated Octel

Company Limited

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

1980

Method/Guideline

Followed:

No Data

Other
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Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species/In Vitro

System:

Other aquatic crustacea

GLP: No Data

Analytical

Monitoring:

Yes

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Brackish Water

Test

Concentrations:

Measured

Nominal and

Measured

Concentrations:

0.01 to 0.2 mg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 15 °C

Photo

(Light/Dark):

Salinity: 34.9

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

All mussels were maintained in flowing sea-water for a minimum of 7 days prior to

test item exposure. Seawater (salinity 34.9 per mille ) was pumped directly from the

sea and supplied at a constant temperature (15 oC) to the test system. At the design

flow rate of 100 mL/min, the 90% replacement time was 7.5 hours. 

Stock solutions were prepared by agitating 22 liters of water with 10 grams of

tetraethyl lead for 4 hours, allowing the excess to settle for 2 hours, and then

pumping the top 20 liters into a sealed stock vessel isolated from direct sunlight. A

concentration of 0.01 to 0.2 mg Pb/L tetraethyl lead was maintained with a variation

of ±10%.

Ten mussels were transferred to a test vessel and maintained in flowing seawater for

24 hours, at which time a pre-selected amount of the stock solution was fed

continuously into the vessel for a period of 96 hours. A control vessel was
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included. Test vessels were regularly monitored for mortality. Water samples were

taken twice daily to analyze tetraethyl lead content. Temperature, dissolved oxygen,

and pH were measured daily. Test animals were fed prior to but not during exposure.

Limit Test:

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 0.1 mg/L Mortality

Results Remarks: The LC50 value was calculated according to the methods of Doudoroff et al. (1951). 

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

No guideline or method was cited, GLP compliance is unknown, individual data on

mortality were not provided, and the exact tested concentrations were

unclear. Additionally, the test was conducted with a non-standard species and

dissolved oxygen, pH, and total organic carbon were not specified.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of some

lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad (eds.), Lead in

the Marine Environment. Proc. Int. Experts Discussion on Lead Occurrence. Fate and

Pollution in the Marine Environment, Rovinj, Yugoslavia: 233-261. Pergamon, Oxford,

UK.
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 Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Not specified

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Invertebrates

Year Study

Performed:

1978

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species/In Vitro

System:

Artemia salina

GLP: Not Applicable

Analytical

Monitoring:

No

Test Type: Static

Test Vessel: Closed

Water Media Type: Saltwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Not specified

Exposure Period: 48 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:
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Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 20 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

The tetraethyl lead solution was prepared by adding 2 ml of product to 1 L of

filtered natural seawater (0.45 μm) and slowly stirring magnetically for 1 hour. The

upper one fourth of the saturated solution was used for preparing experimental

dilutions in natural filtered seawater. Analytical measurement by atomic absorption

spectrophotometry gave a content of 2 mg total lead/L in the stock solution. The

experiment was carried out in the dark, at 20 oC using closed vessels to avoid gasphase leeching of the volatile aklyl lead compounds.

Limit Test:

Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % = 85 ppb Mortality

Results Remarks: The 48-hr LC50 was 85 ppb Pb. One-hundred percent mortality was seen after 48 hours

at 260 ppb Pb. Almost immediate 100% mortality occurred at 770 ppb Pb. No effect
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was seen after 6 hours at 500 ppb Pb. Alkyl leads show higher toxicity than inorganic

lead.

Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability

Remarks:

The test substance was a formulation; composition was not specified. Additionally,

the tested concentrations were unclear; a non-standard species was tested; and missing

experimental details included loading rate, pH, salinity, dissolved oxygen

concentration, total organic carbon levels, and whether the test was conducted in

static or flow-through chambers.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Marchetti, R. 1978. Acute toxicity of alkyl leads to some marine organisms. Marine

Pollution Bulletin, 9: 206-207.

 Acute Toxicity to Aquatic Plants

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Plants

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Not specified

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

1978

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Dunaliella tertiolecta

GLP: Not Applicable

Analytical

Monitoring:

No Data

Test Type: No Data

Test Vessel: Closed

Water Media Type: Saltwater
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Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 48 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 20 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

The tetraethyl lead solution was prepared by adding 2 ml of product to 1 L of

filtered natural seawater (0.45 μm) and slowly stirring magnetically for 1

hour. The upper one fourth of the saturated solution was used for preparing

experimental dilutions in natural filtered seawater. Analytical measurement by

atomic absorption spectrophotometry gave a content of 2 mg total lead/L in the

stock solution. The experiment was carried out at 20 oC using closed vessels to

avoid gas-phase leaching of the volatile compound. The toxicity to algae was

measured as the reduction of photosynthetic activity by the 14C method.

Limit Test:

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:
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Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration

48 Hours EC 50 % = 150 ppb Other

Results Remarks: The 48-hr EC50 was 150 ppb. One-hundred percent reduction of photosynthesis was

seen after 48 hours at 300 ppb. No effects on photosynthesis were observed at

concentrations less than 100 ppb Pb. Complete inhibition of photosynthetic

activity was observed at 500 ppb after 6 hours. Alkyl leads show higher toxicity

than inorganic lead.

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability

Remarks:

The test duration was less than the OECD-recommended length of 72

hours. Additionally, the test substance was a formulation (composition not

specified); the tested concentrations were unclear; a non-standard species was

tested; and missing experimental details included pH, salinity, and dissolved

oxygen concentration.

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Marchetti, R. 1978. Acute toxicity of alkyl leads to some marine

organisms. Marine Pollution Bulletin, 9: 206-207.

 Acute Toxicity to Aquatic Plants

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Plants

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

98% pure, supplied by Ellesmere Port Research Laboratories of the Associated

Octel Company Limited

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Nominal

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

1980
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Method/Guideline

Followed:

Other

Other

Method/Guideline:

Based on a technique developed from the organic productivity assessment method of

Steeman-Nielsen (1952).

Deviations from

Method/Guideline:

No Data

Species: Phaeodactylum sp.

GLP: No Data

Analytical

Monitoring:

Yes

Test Type: Static

Test Vessel: No Data

Water Media Type: Saltwater

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 6 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 15 °C

Photo

(Light/Dark):

Salinity: 34.9

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Seawater (salinity 34.9 per mille ) was pumped directly from the sea and supplied

at a constant temperature (15 oC) to the test system. Stock solutions were

prepared by agitating 22 liters of water with 10 grams of tetraethyl lead for 4

hours, allowing the excess to settle for 2 hours, and then pumping the top 20

liters into a sealed stock vessel isolated from direct sunlight.

A standardized quantity of algae was incubated for 6 hours in the presence of a

range of concentrations of the test item and a standard amount of C14-labeled

carbonate. The accumulation of C14 by the control cultures indicates the

"normal" activity of the algae which can be compared to activity of algae exposed
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to the test item.

Limit Test:

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration

6 Hours EC 50 % = 0.1 mg/L Other

Results Remarks: The 6-hour EC50 value was 0.10 mg Pb/L, based on a reduction in photosynthetic

activity. The EC50 value was calculated according to the methods of Doudoroff et

al. (1951)

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability

Remarks:

The exposure period appears to have been only 6 hours in duration; OECD

guidelines specify a duration of 72 hours. Additionally, no guideline or method

was cited, GLP compliance is unknown, a non-standard test method was used, and

the exact tested concentrations were unclear. 

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of

some lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad

(eds.), Lead in the Marine Environment. Proc. Int. Experts Discussion on Lead

Occurrence. Fate and Pollution in the Marine Environment, Rovinj, Yugoslavia:

233-261. Pergamon, Oxford, UK.
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 11:27:07 AM]

 Acute Toxicity to Aquatic Plants

CAS Number : 78-00-2

Sponsored

Chemical Name :

Plumbane, tetraethylTest Substance - Acute Toxicity To Aquatic Plants

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test

Substance

Comments:

Not specified

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Unknown

Method - Acute Toxicity To Aquatic Plants

Year Study

Performed:

1992

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

No Data

Species: Cyclotella sp.

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:
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pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Growth rate was measured.

Limit Test:

Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

Effect : Exposure

Duration

Exposure

Units

Type % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Basis

for

Effect

Basis for

Concentration

Results Remarks: None specified. The IUCLID summary was mostly in a language other than

English.

Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 4 - Not Assignable

Reliability

Remarks:

The IUCLID summary, which was mostly in a language other than English,

contained insufficient details to assess data adequacy.
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Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Plants

Reference: Primary reference (unavailable): Giri, B.S. and N.A. Devi. 1992. Indian J Exp

Biol 30: 201-204. 

Secondary reference: IUCLID Dataset for CAS No. 78-00-2, available at

http://ecb.jrc.ec.europa.eu/esis/

 Acute Toxicity

CAS Number : 78-00-2

Sponsored Chemical Name

:

Plumbane, tetraethylTest Substance - Acute Toxicity

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test Substance

Comments:

None specified

Sponsored Chemical Result

Type:

Measured

Method - Acute Toxicity

Route of Administration: Oral

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data

Gender: No Data

Number of Animals per

Dose:

Dose: Not specified

Year Study Performed: 1973

Method/Guideline

Followed:

No Data

GLP: Not Applicable

Method/Guideline and

Test Condition Remarks:

None specified

Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

LD = 30 mg/kgbw
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Number of Deaths ( Male

):

Number of Deaths ( Female

):

Number of Deaths ( Total

):

Results Remarks: The oral LDLo was 30 mg/kg.

Conclusion:

Reliability/Data Quality - Acute Toxicity

Reliability: 4 - Not Assignable

Reliability Remarks: The IUCLID summary lacked sufficient information to assess

data adequacy. 

Key Study Sponsor

Indicator:

Reference - Acute Toxicity

Reference: Primary reference (unavailable): Japanese Journal of

Industrial Health, 15: p. 3. 1973. 

Secondary reference: IUCLID Dataset for CAS No. 78-00-2,

available at http://ecb.jrc.ec.europa.eu/esis/

 Acute Toxicity

CAS Number : 78-00-2

Sponsored Chemical Name

:

Plumbane, tetraethylTest Substance - Acute Toxicity

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance

Purity/Composition

and Other Test Substance

Comments:

98% pure, supplied by Houston Chemical Company, Freeport, TX

Sponsored Chemical Result

Type:

Measured

Method - Acute Toxicity

Route of Administration: Oral

Type of Exposure: Gavage

Species: Mouse

Mammalian Strain: Other
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Other Strain: CD-1

Gender: No Data

Number of Animals per

Dose:

10

Dose: 35.12, 52.67, 79.01, and 118.5 mg/kg

Year Study Performed: 1975

Method/Guideline

Followed:

No Data

GLP: Not Applicable

Method/Guideline and

Test Condition Remarks:

Statistical methods used: The methods of Litchfield and

Wilcoxon (1949) were used.

Test method/conditions remarks: Single graded oral doses were

given to groups of 10 animals and 14-day LD50 values were

calculated.

Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value

Description

Value/Lower

Concentration

Upper

Concentration

Units

LD 50 % = 62.5 68.5 mg/kgbw

Number of Deaths ( Male

):

Number of Deaths ( Female

):

Number of Deaths ( Total

):

Results Remarks: Mortalities were 0/10, 2/10, 10/10, and 10/10 at 35.12,

52.67, 79.01, and 118.5 mg/kg, respectively. Clinical signs

at 52.67 mg/kg included hypoactivity from 6 to 24

hours. Clinical signs seen at 79.01 mg/kg included

hypoactivity (from 6 hours until death) and tremors (from 5

to 24 hours, or until death). Clinical signs seen at 118.5

mg/kg consisted of hypoactivity (from 2 hours until death),

tremors (from 3 to 12 hours or until death) and clonic

convulsions (from 2-3 to 5 hours).

Conclusion:

Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks: The sex of test animals was not specified.

Key Study Sponsor
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Indicator:

Reference - Acute Toxicity

Reference: Kennedy, G.L. et al. 1975. Teratogenic evaluation of lead

compounds in mice and rats. Food. Cosmet. Toxicol., 13: 629-

632.