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While small concentrations of test item are not completely 

soluble in water (e.g . 16.6 mg/I), the test item is, on the other 

hand, able to form homogeneous mixtures at high concentrations. 

Due to this behavior, it is not possible to determine a defined 

water solubility of the test item . 

1 Water Solubility 

Summary The test item is partly miscible with/soluble in water, depending 

on the ratios of test item to water applied . 

While small concentrations of test item are not completely 

soluble in water (e.g . 16 .6 mg/I), the test item is, on the other 

hand, able to form homogeneous mixtures at high concentrations. 

Due to this behavior, it is not possible to determine a defined 

water solubility of the test item. 

Physical State At room temperature the test item is a clear viscous darkyellow colored liquid . 

General Remark The test item consists of a mixture of homologues with different 

molecular weights and, in addition to this, probably of different 

secondary components. Every constituent of this mixture contributes to a different degree to overall solubility, depending on 

its own individual solubility and its mass fraction in the test 

item. For determination of the water solubility, the preparation 

of saturated solutions of the test item in water is required . 

Since this is not possible for this test item, the solubility in water can only be investigated by a visual method using different 

proportions of test item and water. 

Pre-testing 16.9 mg of the test item were mixed with 10 ml, 13.6 mg of the 

test item were mixed with 100 ml and 16.6 mg of the test item 

were mixed with 1000 ml of water at 23°C. After the addition of 

water, the test item was floating on the surface of the water 

and after stirring overnight at 23°C, the test item was finely dispersed in the water phases, resulting in turbid solutions . 

Thus, the water solubility of the test item was estimated to be < 

16 .6 mg/I . 

Remark: This pre-testing merely shows that 16.6 mg of the test 

item are not completely soluble in one liter of water. As the test 

item is not a pure compound but a mixture of compounds with 

possibly different properties, it may be that the water solubility 

of fractions of the test item is higher than 16.6 mg/I. 

Method The determinations were done according to OECD Guideline 

for the Testing of Chemicals, 105, flask method . 
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Although the water solubility was estimated to be < 16.6 mg/l, 

the flask method was carried out instead of the column elution 

method, because the test item is not a defined single compound. 

As it was not possible to produce saturated solutions without 

undissolved amounts of test item according to the flask 

method, additional preparations of the test item with different 

amounts of water were carried out to investigate the miscibility 

and the solubility behavior of the test item. 

Reagent Deminerafized water (Milli-Q System) 

Analytical Method Preparations according to the flask method (OECD 105): 

About 100 mg and about 1000 mg of the test item were each 

mixed with 100 ml of water and then stirred at room temperature (23°C) for 24h . Other preparations with the same concentrations were stirred for 48 h and for 72 h . Afterwards the mixtures were conditioned at 20.0°C ± 0.5 °C for 24 h. 

After that, the mixtures were strongly turbid. The undissolved 

amounts of the test item could not be removed, neither by filtration nor by centrifugation . Thus, it was not possible to 

achieve defined saturated solutions of test item in water. 

Preparations for visual investigation : 

The test item and water were mixed in different ratios and agitated on a roller mixer at room temperature (T = 23°C) to investigate the solubility / miscibility behaviour of the test item. 

The following mixtures were prepared : 

ratio m test item m water I m ti / m mixture ~ Observations 

191 f9] i [9 / 100 g] i 

1 :9 1 .01 ~ 9 .02 ' 10.1 i After agitating overnight, the homogeneous yellowish and jelly-like mixture was turbid with inclusions 

of air . 

1 :1 2.07 ~ 2.17 I 48.8 After agitating overnight, the homogeneous yellow ~ 

and jelly-like mixture was turbid with inclusions of 

air. 

9:1 j 4.51 , 0.54 89.3 After agitating overnight, the homogeneous yellowbrown and jelly-like mixture was slightly turbid with 

inclusions of air . 

Result The test item is partly miscible with/soluble in water, depending on the ratios of test item to water applied . 

While small concentrations of the test item are not completely 

soluble in water (e.g . 16 .6 mg/I, see pre-testing), the test item 

is, on the other hand, able to form homogeneous mixtures at 
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high concentrations . 

Due to this behavior, it is not possible to determine a defined 

water solubility of the test item. 

Date of test 
p. 5 

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p. 6 

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GLP Comnliance Statement 
p. 7 

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