### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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# Submission Report

Submission Information

Submission ID : 25349302 Submission Name : Plumbane, tetraethylAR Number :



## Chemical Information

CAS Number : 78-00-2 Ninth Collective Index Name : Plumbane, tetraethyl

## Submitter Information

Submitter's Name : EPA

Address : City : Washington

State : DC

ZIP/Postal Code : Country : Type of Submitter : Company 

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## Sponsor Information
Sponsor's Name : EPA

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### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] E-Mail Address : Type of Sponsor : Company 

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## Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Composition of test item (w/w): 61.3% tetraethyl lead; 35.68% 1,2-dibromoethane; 0.026% blue dye; 2.93% kerosene and inerts; 0.06% antioxidant AO-29.

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1975

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Pimephales promelas

GLP: No

Analytical Monitoring: No Data

Test Type: Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations: 0, 0.005, 0.0075, 0.01, 0.025, 0.05, 0.075, or 0.1 mg/L

Exposure Period: 96 Hours

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Vehicle Used: Yes

Vehicle Name: Acetone

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value: Value/Lower Range: = 7.8

Test Temperature and Units: Value/Lower Range: 19.8 Upper Range: 20.2 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: The test material, as either a 10, 0.1 or 0.01% solution in acetone, was introduced into all-glass vessels containing ten fathead minnows in 15 liters of water. Fish were not fed during exposure. No aeration was provided during the test. Dissolved oxygen and pH were measured at the beginning of exposure. Calculation of the 96-hour LC50 was via straight line graphical interpolation. An acetone control was included, and prepared by adding, to clean supply water, a volume of acetone equal to the greatest volume of test solution used. A negative water control was also included. Test species: Pimephales promelas (Fathead minnow); 3.7 cm standard length.

Limit Test: No



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.037 mg/L Mortality

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Results Remarks: The test temperature was maintained at 20 ± 0.2 oC. The pH was 7.8. Mortality was 100% at the two highest test concentrations, 90% at the third-highest concentration, and 0% at all other tested concentrations. Tetraethyl lead exhibits extreme acute toxicity to fathead minnows.


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks: No guideline or method was cited, and GLP compliance is unknown. Additionally, it is unclear whether analytical monitoring was performed, and dissolved oxygen concentrations and total organic carbon was not specified.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: TSCATS submission (OTS0555568, Doc ID 88-920010086, received 10/27/92): Haskell Laboratory, DuPont Chemical Company. 1975. Initial submission: Report of 96-hr LC50 of Lead, Tetraethyl in Fathead Minnows with Cover Letter Dated 101592.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: 98% pure, supplied by Ellesmere Port Research Laboratories of the Associated Octel Company Limited

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1980

Method/Guideline Followed: No Data

Other Method/Guideline: see below

Deviations from Method/Guideline: Yes

Species: Pleuronectes platessa

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] GLP: No Data

Analytical Monitoring: Yes

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: Measured

Nominal and Measured Concentrations: 0, 0.02 to 0.2 mg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 15 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Test type: Acute Toxicity to Fish - Flow-through Test species: Pleuronectes platessa (European plaice; saltwater fish). Mean length: 5.2 cm; mean weight: 2.8 g. Source: Tor Bay, S. Devon; obtained via otter trawl. Test method/conditions remarks: All fish were maintained in flowing sea-water for a minimum of 7 days prior to test item exposure. Seawater (salinity 34.9 per mille ) was pumped directly from the sea and supplied at a constant temperature (15 oC) to the test system. At the design flow rate of 100 mL/min, the 90% replacement time was
7.5 hours.

Stock solutions were prepared by agitating 22 liters of water with 10 grams of tetraethyl lead for 4 hours, allowing the excess to settle for 2 hours, and then pumping the top 20 liters into a sealed stock vessel isolated from direct sunlight. A concentration of 0.02 to 0.2 mg Pb/L tetraethyl lead was maintained with a variation
of ±10%.

Ten fish were transferred to a test vessel and maintained in flowing seawater for 24 hours, at which time a pre-selected amount of the stock solution was fed continuously into the vessel for a period of 96 hours. A control vessel was included. Test vessels were regularly monitored for mortality. Water samples were taken twice daily to analyze tetraethyl lead content. Temperature, dissolved oxygen, and pH were measured daily. Test animals were fed prior to but not during exposure.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.23 mg/L Mortality

Results Remarks: The 96-hour LC50 value was 0.23 mg Pb/L. The LC50 value was calculated according to the methods of Doudoroff et al. (1951). The study authors reported that the results show that the plaice is more resistant to the effects of tetraethyl lead than to tetramethyl lead.


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks: No guideline or method was cited, GLP compliance is unknown, individual data on mortality were not provided, and the exact tested concentrations were
unclear. Dissolved oxygen, pH, and total organic carbon were not specified. Additionally, the test was performed with a non-standard marine species.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of some lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad (eds.), Lead in the Marine Environment. Proc. Int. Experts Discussion on Lead Occurrence. Fate and Pollution in the Marine Environment, Rovinj, Yugoslavia: 233-261. Pergamon, Oxford,

UK.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] 

## Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Purity not specified

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1964

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Lepomis macrochirus

GLP: No

Analytical Monitoring: No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: GLP compliant?: The study pre-dates GLP.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.2 mg/L Mortality

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks: The IUCLID summary lacked sufficient information to assess data adequacy.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Primary reference (not available): Makina, J.F. 1964. Toxicology of petrochemicals in the aquatic environment - water and sewage works, vol 3, pp. 456-460. Secondary reference: IUCLID Dataset for CAS No. 78-00-2, available at http://ecb.jrc.ec.europa.eu/esis/



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Purity not provided; test substance described as a commercial product, "TEL-CB"

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1978

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: Yes

Species: Morone sp.

GLP: No

Analytical Monitoring: No

Test Type: No Data

Test Vessel: Closed

Water Media Type: Saltwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 48 Hours

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 20 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Test species: 6-mm larvae of Morone labrax (European seabass) The tetraethyl lead solution was prepared by adding 2 ml of product to 1 L of filtered natural seawater (0.45 μm) and slowly stirring magnetically for 1 hour. The upper one fourth of the saturated solution was used for preparing experimental dilutions in natural filtered seawater. Analytical measurement by atomic absorption spectrophotometry gave a content of 2 mg total lead/L for the stock solution. Theexperiment was carried out in the dark, at 20 oC, using closed vessels to avoid gasphase leaching of the volatile alkyl lead compound.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % = 65 ppb Mortality

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Results Remarks: The 48-hr LC50 was 65 ppb Pb. One-hundred percent mortality was seen after 48 hours at 130 ppb Pb. All larvae died almost immediately at concentrations of 650 ppb
Pb. There were no mortalities at 10 ppb Pb. Alkyl leads show higher toxicity than inorganic lead.


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks: The test duration was less than the OECD-recommended length of 96
hours. Additionally, the tested concentrations were unclear; a non-standard fish species was tested; and missing experimental details included loading rate, pH, salinity, dissolved oxygen concentration, total organic carbon levels, and whether the test was conducted in static or flow-through chambers.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Marchetti, R. 1978. Acute toxicity of alkyl leads to some marine organisms. Marine Pollution Bulletin, 9: 206-207.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Purity not specified

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1977

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Other

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Other Species: various - see below

GLP: No

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Saltwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 30 Minutes

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: GLP compliant?: The study pre-dates GLP. Test species: Gobius niger (black goby), Trachurus trachurus (Atlantic horse mackerel), Mugil cephalus (striped mullet), Atherina boyeri (sand smelt), Trachinus draco (greater weever) Analytical monitoring: Not specified Exposure duration: 30 minutes Concentrations tested: Not specified Test method/conditions remarks: The IUCLID summary of the study was mostly in a language other than English.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

Results Remarks: The IUCLID summary of the study was mostly in a language other than English.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks: Test duration is less than the OECD-recommended length of 96 hours and tests were conducted using non-standard fish species. Additionally, the IUCLID summary, which was mostly in a language other than English, lacked study details. The IUCLID summary noted an assigned Klimisch code of 2 for the study.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Primary reference (not available): Giaccio, M. 1977. Quad. Merceol. 16: 55-62. Secondary reference: IUCLID Dataset for CAS No. 78-00-2, available at http://ecb.jrc.ec.europa.eu/esis/



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethyl-

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Test Substance Purity/Composition and Other Test Substance Comments: Purity not specified

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1954

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Lepomis macrochirus

GLP: No

Analytical Monitoring: No

Test Type: Static

Test Vessel: No Data

Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 48 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity: 38-81

Dissolved Oxygen: 5 ppm or greater

pH Value: Value/Lower Range: = 6.9 Upper Range: 7.5

Test Temperature and Units: Value/Lower Range: 20 °C

Photo (Light/Dark):

Salinity:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] TOC:

Water Hardness: 84 - 163 ppm

Method/Guideline Test Conditions Remarks: Method/guideline followed: Not specified Test type: Acute Toxicity to Fish - Static GLP compliant?: The study pre-dates GLP. Test species: Bluegill sunfish (Lepomis macrochirus), obtained from the Pennsylvania State Hatchery (Torresdale, PA) and Hiram People's Hatchery (New Providence, PA). Average length: 7 cm. Average weight: 5 g.

Test method/conditions remarks: The test chemical was added to the 5-gallon test jar at the start of the test period. No subsequent additions were made during the course
of the test. Test jars were filled to the 15-liter mark and placed in a 20 oC thermostatically-controlled water bath. Carbon-filtered Philadelphia tap water was
used. Fish were acclimatized to the test water and temperature for 10 days prior to the test period. Ten fish per jar were tested. Based on the average fish weight of 5 g and the number of fish per unit volume of test solution (10 fish/15 L), the loading rate was 3.3 g fish/L, which exceeds the OECD-recommended maximum loading rate of 1 g for static tests. Characteristics of dilution water: total alkalinity as CaCO3 = 38-81 ppm; pH = 6.9-7.5; total hardness as CaCO3 = 84-163 ppm; specific conductivity = 228-301 micromhos; dissolved solids = 154-210 ppm; sulfates as SO4 = 56-98 ppm; chlorides as Cl = 3-26 ppm. Dissolved oxygen concentration was maintained at 5 ppm or greater throughout the test by gentle aeration.

Mortality was recorded after 24 and 48 hours. The LC50 was obtained by interpolation from a graph of the logarithm of the concentration versus the percentage mortality.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % = 1.4 mg/L Mortality

24 Hours LC 50 % = 2 mg/L Mortality

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Results Remarks: The 48-hr LC50 value was 1.4 mg lead/L. The 24-hour LC50 value was 2.0 mg lead/L.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 3 - Not Valid

Reliability Remarks: Test duration is less than the OECD-recommended length of 96 hours; no analytical monitoring was performed; loading rate exceeded the OECD-recommended limit.

The IUCLID summary noted an assigned Klimisch code of 2 for the study.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Turnbull, H. et al. 1954. Toxicity of various refinery materials to fresh water
fish. Ind. Eng. Chem. 46: 324-333.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Composition of test item (w/w): 61.3% tetraethyl lead; 35.68% 1,2-dibromoethane; 0.026% blue dye; 2.93% kerosene and inerts; 0.06% antioxidant AO-29.

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1976

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Lepomis macrochirus

GLP: No

Analytical Monitoring: No Data

Test Type: Static

Test Vessel: No Data

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Water Media Type: Freshwater

Test Concentrations: No Data

Nominal and Measured Concentrations: 0, 0.010, 0.025, 0.050, 0.075, 0.10, 0.25, 0.5 mg/L

Exposure Period: 96 Hours

Vehicle Used: Yes

Vehicle Name: Acetone

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen: 8.6 to 8.7 ppm

pH Value: Value/Lower Range: = 7.7 Upper Range: 8

Test Temperature and Units: Value/Lower Range: 19.8 Upper Range: 20.2 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: The test material, as either a 0.1 or 0.01% solution in acetone, was introduced into all-glass vessels containing ten bluefish in 15 liters of water. Fish were not fed for 48 hours prior to testing, nor during exposure. No aeration was provided during
the test. Dissolved oxygen and pH were measured at the beginning of

exposure. Calculation of the 96-hour LC50 was via straight line graphical interpolation. An acetone control was included, and prepared by adding, to clean supply water, a volume of acetone equal to the greatest volume of test solution
used. A negative water control was also included.

Limit Test: No



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.15 mg/L Mortality

Results Remarks: The test temperature was maintained at 20 ± 0.2 oC. The pH measurements across tested concentrations ranged from 7.7 to 8.0 and dissolved oxygen ranged from 8.6 to 8.7

ppm. Mortality was 100% at 0.25 and 0.50 mg/L, 10% at 0.10 mg/L, and 0% at all other tested concentrations. Tetraethyl lead exhibits extreme acute toxicity to bluegill
sunfish.


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks: No guideline or method was cited, and GLP compliance is unknown. Additionally, it is unclear whether analytical monitoring was performed and total organic carbon was not specified.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: TSCATS submission (OTS0546521, Doc ID 88-920010136, received 10/27/92): Haskell Laboratory, DuPont Chemical Company. 1976. Initial submission: 96-hr LC50 to Bluegill Sunfish with Cover Letter Dated 101592.



## Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: 98% pure, supplied by Ellesmere Port Research Laboratories of the Associated Octel Company Limited

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study 1980

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: Yes

Species/In Vitro System: Crangon crangon

GLP: No Data

Analytical Monitoring: Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Saltwater

Test Concentrations:

Nominal and Measured Concentrations: 0.01 to 0.2 mg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 15 °C

Photo (Light/Dark):

Salinity: 34.9

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Test species: Crangon crangon (brown shrimp). Mean length: 4.8 cm; mean weight: 1.1

g. Source: Tor Bay, S. Devon; obtained via push net. All shrimp were maintained in flowing sea-water for a minimum of 7 days prior to test item exposure. Seawater (salinity 34.9 per mille ) was pumped directly from the sea and supplied at a constant temperature (15 oC) to the test system. At the design flow rate of 100 mL/min, the 90% replacement time was 7.5 hours.

Stock solutions were prepared by agitating 22 liters of water with 10 grams of tetraethyl lead for 4 hours, allowing the excess to settle for 2 hours, and then

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] pumping the top 20 liters into a sealed stock vessel isolated from direct sunlight. A concentration of 0.01 to 0.2 mg Pb/L tetraethyl lead was maintained with a variation
of ±10%.

Ten shrimp were transferred to a test vessel and maintained in flowing seawater for 24 hours, at which time pre-selected amount of the stock solution was fed continuously into the vessel for a period of 96 hours. A control vessel was included. Test vessels were regularly monitored for mortality. Water samples were taken twice daily to analyze tetraethyl lead content. Temperature, dissolved oxygen, and pH were measured daily. Test animals were fed prior to but not during exposure.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.02 mg/L Mortality

Results Remarks: The 96-hour LC50 value was 0.02 mg Pb/L. The LC50 value was calculated according to the methods of Doudoroff et al. (1951).


### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks: No guideline or method was cited, GLP compliance is unknown, individual data on mortality were not provided, and the exact tested concentrations were
unclear. Additionally, the test employed a non-standard species and dissolved oxygen, pH, and total organic carbon were not specified.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of some lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad (eds.), Lead in the Marine Environment. Proc. Int. Experts Discussion on Lead Occurrence. Fate and Pollution in the Marine Environment, Rovinj, Yugoslavia: 233-261. Pergamon, Oxford,

UK.



## Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Concentration of tetraethyl lead not specified; lead content: 0.6247 g Pb/mL; clear red liquid; solvent/carrier: reagent grade absolute ethanol

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed: 1976

Method/Guideline Followed: Other

Other Method/Guideline: Methods of Butler et al. (1960) and Butler (1965)

Deviations from Method/Guideline:
Species/In Vitro System: Other aquatic crustacea

GLP: No

Analytical Monitoring: Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Saltwater

Test Concentrations: Both

Nominal and Measured Concentrations: Nominal: 0 (solvent control), 1.0, 10, 56, 100, 200 μg Pb/L; measured: 0, 1.0, 5.2, 36, 72, 131 μg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 28 Upper Range: 30 °C

Photo (Light/Dark):

Salinity: 30

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Test species: Eastern oyster (Crassostrea virginica), 2-4 cm from umbo to distal valve edge, wild-caught (Pensacola, FL) Oysters were held in tanks of flowing, natural seawater of ambient salinity and temperature for a minimum of two weeks before testing. Oysters were cleaned of attached organisms and ground by hand on a fine-grit grinding wheel to remove approximately 2-5 mm of peripheral shell. Six groups of ten oysters each were exposed to each test concentration for 96 hours. The test was conducted in a glass system that was covered, where feasible, to reduce volatilization of the test
substance. The system was 18 L, received seawater at a rate of 30 L/hour, and ~99% replacement occurred every 3 hours. New shell growth was measured to the nearest 0.1 mm using a binocular dissecting scope equipped with a micrometer.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours EC 50 % > 1 ug/L Other

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Results Remarks: The 96-hour EC50 value for shell growth was > 1 but < 5.2 μg Pb/L.

The percentage reduction of new shell growth of exposed oysters, as compared to control oysters was 43, 85, 85, 85, and 100% at concentrations of 1.0, 5.2, 36, 72, and 131 μg Pb/L, respectively. Salinity was 30-32 per mille and temperature was 28-
30 °C.

Shell growth of oysters exposed to the test substance in seawater was reduced by more than 50% in concentrations of tetraalkyl lead ≥ 5.2 μg Pb/L during an acute

exposure. 

### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability Remarks: The concentration of tetraethyl lead in the test substance was not specified.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: TSCATS submission (OTS0200597, Doc ID 88-7800249, received 12/10/79): Haskell Laboratory, EI DuPont de Nemours & Company. 1978. Bioaccumulation and Depuration of Lead by Eastern Oysters (Crassostrea virginica) Exposed to a Tetraethyl Lead Motor Fuel Antiknock Compound with Attachments and Cover Letter Dated 120379, conducted by EG&G Bionomics, Marine Research Laboratory, Appendix A.



## Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: 98% pure, supplied by Ellesmere Port Research Laboratories of the Associated Octel Company Limited

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed: 1980

Method/Guideline Followed: No Data

Other

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Method/Guideline:

Deviations from Method/Guideline: No Data

Species/In Vitro System: Other aquatic crustacea

GLP: No Data

Analytical Monitoring: Yes

Test Type: Flow-through

Test Vessel: No Data

Water Media Type: Brackish Water

Test Concentrations: Measured

Nominal and Measured Concentrations: 0.01 to 0.2 mg Pb/L

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 15 °C

Photo (Light/Dark):

Salinity: 34.9

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: All mussels were maintained in flowing sea-water for a minimum of 7 days prior to test item exposure. Seawater (salinity 34.9 per mille ) was pumped directly from the sea and supplied at a constant temperature (15 oC) to the test system. At the design flow rate of 100 mL/min, the 90% replacement time was 7.5 hours.

Stock solutions were prepared by agitating 22 liters of water with 10 grams of tetraethyl lead for 4 hours, allowing the excess to settle for 2 hours, and then pumping the top 20 liters into a sealed stock vessel isolated from direct sunlight. A concentration of 0.01 to 0.2 mg Pb/L tetraethyl lead was maintained with a variation
of ±10%.

Ten mussels were transferred to a test vessel and maintained in flowing seawater for 24 hours, at which time a pre-selected amount of the stock solution was fed continuously into the vessel for a period of 96 hours. A control vessel was

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] included. Test vessels were regularly monitored for mortality. Water samples were taken twice daily to analyze tetraethyl lead content. Temperature, dissolved oxygen, and pH were measured daily. Test animals were fed prior to but not during exposure.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 0.1 mg/L Mortality

Results Remarks: The LC50 value was calculated according to the methods of Doudoroff et al. (1951).



### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks: No guideline or method was cited, GLP compliance is unknown, individual data on mortality were not provided, and the exact tested concentrations were
unclear. Additionally, the test was conducted with a non-standard species and dissolved oxygen, pH, and total organic carbon were not specified.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of some lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad (eds.), Lead in the Marine Environment. Proc. Int. Experts Discussion on Lead Occurrence. Fate and Pollution in the Marine Environment, Rovinj, Yugoslavia: 233-261. Pergamon, Oxford,

UK.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] 

## Acute Toxicity to Aquatic Invertebrates

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Invertebrates

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Not specified

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Invertebrates

Year Study Performed: 1978

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species/In Vitro System: Artemia salina

GLP: Not Applicable

Analytical Monitoring: No

Test Type: Static

Test Vessel: Closed

Water Media Type: Saltwater

Test Concentrations: No Data

Nominal and Measured Concentrations: Not specified

Exposure Period: 48 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 20 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: The tetraethyl lead solution was prepared by adding 2 ml of product to 1 L of filtered natural seawater (0.45 μm) and slowly stirring magnetically for 1 hour. The upper one fourth of the saturated solution was used for preparing experimental dilutions in natural filtered seawater. Analytical measurement by atomic absorption spectrophotometry gave a content of 2 mg total lead/L in the stock solution. Theexperiment was carried out in the dark, at 20 oC using closed vessels to avoid gasphase leeching of the volatile aklyl lead compounds.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Invertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % = 85 ppb Mortality

Results Remarks: The 48-hr LC50 was 85 ppb Pb. One-hundred percent mortality was seen after 48 hours at 260 ppb Pb. Almost immediate 100% mortality occurred at 770 ppb Pb. No effect

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] was seen after 6 hours at 500 ppb Pb. Alkyl leads show higher toxicity than inorganic
lead.


### Reliability/Data Quality - Acute Toxicity To Aquatic Invertebrates

Reliability: 3 - Not Valid

Reliability Remarks: The test substance was a formulation; composition was not specified. Additionally, the tested concentrations were unclear; a non-standard species was tested; and missing experimental details included loading rate, pH, salinity, dissolved oxygen concentration, total organic carbon levels, and whether the test was conducted in static or flow-through chambers.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Invertebrates

Reference: Marchetti, R. 1978. Acute toxicity of alkyl leads to some marine organisms. Marine Pollution Bulletin, 9: 206-207.



## Acute Toxicity to Aquatic Plants

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Not specified

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed: 1978

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Dunaliella tertiolecta

GLP: Not Applicable

Analytical Monitoring: No Data

Test Type: No Data

Test Vessel: Closed

Water Media Type: Saltwater

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 48 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 20 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: The tetraethyl lead solution was prepared by adding 2 ml of product to 1 L of filtered natural seawater (0.45 μm) and slowly stirring magnetically for 1
hour. The upper one fourth of the saturated solution was used for preparing experimental dilutions in natural filtered seawater. Analytical measurement by atomic absorption spectrophotometry gave a content of 2 mg total lead/L in the stock solution. The experiment was carried out at 20 oC using closed vessels to avoid gas-phase leaching of the volatile compound. The toxicity to algae was measured as the reduction of photosynthetic activity by the 14C method.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

48 Hours EC 50 % = 150 ppb Other

Results Remarks: The 48-hr EC50 was 150 ppb. One-hundred percent reduction of photosynthesis was seen after 48 hours at 300 ppb. No effects on photosynthesis were observed at concentrations less than 100 ppb Pb. Complete inhibition of photosynthetic activity was observed at 500 ppb after 6 hours. Alkyl leads show higher toxicity than inorganic lead.


### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability Remarks: The test duration was less than the OECD-recommended length of 72
hours. Additionally, the test substance was a formulation (composition not specified); the tested concentrations were unclear; a non-standard species was tested; and missing experimental details included pH, salinity, and dissolved oxygen concentration.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Marchetti, R. 1978. Acute toxicity of alkyl leads to some marine organisms. Marine Pollution Bulletin, 9: 206-207.



## Acute Toxicity to Aquatic Plants

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: 98% pure, supplied by Ellesmere Port Research Laboratories of the Associated Octel Company Limited

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Nominal



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed: 1980

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Method/Guideline Followed: Other

Other Method/Guideline: Based on a technique developed from the organic productivity assessment method of Steeman-Nielsen (1952).

Deviations from Method/Guideline: No Data

Species: Phaeodactylum sp.

GLP: No Data

Analytical Monitoring: Yes

Test Type: Static

Test Vessel: No Data

Water Media Type: Saltwater

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 6 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 15 °C

Photo (Light/Dark):

Salinity: 34.9

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Seawater (salinity 34.9 per mille ) was pumped directly from the sea and supplied at a constant temperature (15 oC) to the test system. Stock solutions were prepared by agitating 22 liters of water with 10 grams of tetraethyl lead for 4 hours, allowing the excess to settle for 2 hours, and then pumping the top 20 liters into a sealed stock vessel isolated from direct sunlight.

A standardized quantity of algae was incubated for 6 hours in the presence of a range of concentrations of the test item and a standard amount of C14-labeled
carbonate. The accumulation of C14 by the control cultures indicates the "normal" activity of the algae which can be compared to activity of algae exposed

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] to the test item.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

6 Hours EC 50 % = 0.1 mg/L Other

Results Remarks: The 6-hour EC50 value was 0.10 mg Pb/L, based on a reduction in photosynthetic activity. The EC50 value was calculated according to the methods of Doudoroff et al. (1951)


### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 3 - Not Valid

Reliability Remarks: The exposure period appears to have been only 6 hours in duration; OECD guidelines specify a duration of 72 hours. Additionally, no guideline or method was cited, GLP compliance is unknown, a non-standard test method was used, and the exact tested concentrations were unclear.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Maddock, B.G. and D. Taylor. 1980. The acute toxicity and bioaccumulation of some lead alkyl compounds in marine animals. In: M. Branika and Z. Konrad (eds.), Lead in the Marine Environment. Proc. Int. Experts Discussion on Lead Occurrence. Fate and Pollution in the Marine Environment, Rovinj, Yugoslavia:
233-261. Pergamon, Oxford, UK.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] 

## Acute Toxicity to Aquatic Plants

CAS Number : 78-00-2

Sponsored Chemical Name : Plumbane, tetraethyl

### Test Substance - Acute Toxicity To Aquatic Plants

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: Not specified

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Unknown



### Method - Acute Toxicity To Aquatic Plants

Year Study Performed: 1992

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline: No Data

Species: Cyclotella sp.

GLP: No Data

Analytical Monitoring: No Data

Test Type: No Data

Test Vessel: No Data

Water Media Type: No Data

Test Concentrations: No Data

Nominal and Measured Concentrations:
Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:
Alkalinity:

Dissolved Oxygen:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Growth rate was measured.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Plants

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

Effect : Exposure Duration Exposure Units Type % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Basis for Effect Basis for Concentration

Results Remarks: None specified. The IUCLID summary was mostly in a language other than English.


### Reliability/Data Quality - Acute Toxicity To Aquatic Plants

Reliability: 4 - Not Assignable

Reliability Remarks: The IUCLID summary, which was mostly in a language other than English, contained insufficient details to assess data adequacy.

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Key Study Sponsor Indicator: 

### Reference - Acute Toxicity To Aquatic Plants

Reference: Primary reference (unavailable): Giri, B.S. and N.A. Devi. 1992. Indian J Exp Biol 30: 201-204. Secondary reference: IUCLID Dataset for CAS No. 78-00-2, available at http://ecb.jrc.ec.europa.eu/esis/



## Acute Toxicity

CAS Number : 78-00-2

Sponsored Chemical Name

: Plumbane, tetraethyl

### Test Substance - Acute Toxicity

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: None specified

Sponsored Chemical Result Type: Measured



### Method - Acute Toxicity

Route of Administration: Oral

Type of Exposure: No Data

Species: Rat

Mammalian Strain: No Data

Gender: No Data

Number of Animals per Dose:

Dose: Not specified

Year Study Performed: 1973

Method/Guideline Followed: No Data

GLP: Not Applicable

Method/Guideline and Test Condition Remarks: None specified



### Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value Description Value/Lower Concentration Upper Concentration Units

LD = 30 mg/kgbw

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Number of Deaths ( Male ):
Number of Deaths ( Female ):
Number of Deaths ( Total ):

Results Remarks: The oral LDLo was 30 mg/kg.

Conclusion:



### Reliability/Data Quality - Acute Toxicity

Reliability: 4 - Not Assignable

Reliability Remarks: The IUCLID summary lacked sufficient information to assess data adequacy.

Key Study Sponsor Indicator: 

### Reference - Acute Toxicity

Reference: Primary reference (unavailable): Japanese Journal of Industrial Health, 15: p. 3. 1973. Secondary reference: IUCLID Dataset for CAS No. 78-00-2, available at http://ecb.jrc.ec.europa.eu/esis/



## Acute Toxicity

CAS Number : 78-00-2

Sponsored Chemical Name

: Plumbane, tetraethyl

### Test Substance - Acute Toxicity

Test Substance: (78-00-2) Plumbane, tetraethylTest Substance Purity/Composition and Other Test Substance Comments: 98% pure, supplied by Houston Chemical Company, Freeport, TX

Sponsored Chemical Result Type: Measured



### Method - Acute Toxicity

Route of Administration: Oral

Type of Exposure: Gavage

Species: Mouse

Mammalian Strain: Other

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Other Strain: CD-1

Gender: No Data

Number of Animals per Dose: 10

Dose: 35.12, 52.67, 79.01, and 118.5 mg/kg

Year Study Performed: 1975

Method/Guideline Followed: No Data

GLP: Not Applicable

Method/Guideline and Test Condition Remarks: Statistical methods used: The methods of Litchfield and Wilcoxon (1949) were used.

Test method/conditions remarks: Single graded oral doses were given to groups of 10 animals and 14-day LD50 values were
calculated.



### Test Results - Acute Toxicity

Concentration (LC/LD) : LC/LD % Value Description Value/Lower Concentration Upper Concentration Units

LD 50 % = 62.5 68.5 mg/kgbw

Number of Deaths ( Male ):
Number of Deaths ( Female ):
Number of Deaths ( Total ):

Results Remarks: Mortalities were 0/10, 2/10, 10/10, and 10/10 at 35.12, 52.67, 79.01, and 118.5 mg/kg, respectively. Clinical signs at 52.67 mg/kg included hypoactivity from 6 to 24
hours. Clinical signs seen at 79.01 mg/kg included hypoactivity (from 6 hours until death) and tremors (from 5 to 24 hours, or until death). Clinical signs seen at 118.5 mg/kg consisted of hypoactivity (from 2 hours until death), tremors (from 3 to 12 hours or until death) and clonic convulsions (from 2-3 to 5 hours).

Conclusion:



### Reliability/Data Quality - Acute Toxicity

Reliability: 2 - Valid With Restrictions

Reliability Remarks: The sex of test animals was not specified.

Key Study Sponsor

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 11:27:07 AM] Indicator: 

### Reference - Acute Toxicity

Reference: Kennedy, G.L. et al. 1975. Teratogenic evaluation of lead compounds in mice and rats. Food. Cosmet. Toxicol., 13: 629-
632.