### Page 2 of 17 COPPER 8-QUINOLINOLATE REGISTRATION REVIEW DECISION

PRODUCT CHEMISTRY, E NVIRONMENTAL FATE AND ECOLOGICAL EFFECTS SUMMARY

PC Code: 024002 ; Case No.: 5118

Introduction:

Copper-8-quinolinolate, also known as oxine-cop per, is an organometallic compound. It is a nonionizable, chelated compound made by a chemical reaction be tween inorganic copper and the organic 8-hydroxyquinolin e. Copper-8-quinolinolate ( ≥ 99.5%) is an odorless olive green crystalline powder that is insoluble in water (0.0 7 mg/L at 25oC) with a ne gligible vapor pressure (6.3 x 10 -12 

### mm Hg).

It has numerous and diverse uses. It is us ed in formulating antiseptics, deodorants, antiperspirants and fungicides. It is registered as an antimicrobi al fungicide for control of decay, sapstain, mold, rot, mildew and certain w ood-boring insects (e.g., termites and powder-post beetles) in various preserva tive applications. It is approved for use primarily in industrial/commercial wood preser vation (Use Site Category X - wood preservatives ) for nonpressure and pressure treatments to control saps tain in freshly cut or debarked lumber, and for protection against mold and decay in unfinishe d wood and various wood pr oducts. It may also be used as a protective wood coating and water-re pellent when applied to new and aged wood. It is intended mainly for exterior above-ground wood us e applications and certain interior structural wood construction, including: millwork, sills/ba seboards, siding, wooden components of decks, playsets, fences, outdoor furniture, shingles, stru ctural lumber, plywood, composites and interior boat holds. Treated wood material s may be used in contact with fruit, vegetables and other foodstuffs (indirect food contact) in areas such as greenhouses for greenhouse items, produce picking boxes/containers, mushro om trays and vegetable stakes.

Copper -8-quinolinolate is also used industrially for materi als preservation (Use Site Category VII - materials preservatives ) as a fungistatic dispersion incorporated into manufactured goods including: paper/paperboard , adhesives, paints and cellulose-based cordage/textiles. Copper 8-quinoli nolate is additionally used to control mold and mildew on industrial textiles (mai nly military-issued or government-spe cified) such as cloth and webbing used in military tentage, tarps, canvas, burlap, ro pe, leather and nets (non-aquatic uses only). It may be used as a water-repellent coating for text iles as well. Copper -8 -quinolinolate is used on hard and porous environmental contact surfaces to remediate mold, mildew, and fungal decay (e.g., outdoor painted surfaces, tile, brick/concrete, glass, metal, plastic, leather, wood, and asphalt shingles).

The chemical is also used commercially in food handling settings as a surface disinfectant to control po tato ring rot in planters , seed handling equipment, seed cutters, storage

### Page 3 of 17 areas and transportation equipment such as ra ilroad cars and trucks (Use Site Category II- food handling/storage establishments, premises, and equipment ).

Copper -8-quinolinolate was first registered w ith the Agency as a pesticide on January 5, 1956. Currently, there are 29 registered copper- 8-quinolinolate products which are listed in Table 1. There are no Special Local Need (SLN ) registrations under Section 24(c) of FIFRA. The Reregistration Eligibility Decision (RED) fo r copper 8-quinolinolate was issued by EPA on September 26, 2007. The technical grade active ingredients (TGAIs) are powder concentrates registered to two companies, Tanabe U.S.A., Inc. (96% a.i.) and Osmose, Inc. (96% a.i.). These are technical chemical source products for form ulation in to manufactur ing wood preservatives and materials preservative end-use products requ iring EPA registration. An additional product, approved in 2006, is registered to James Hard ie Building Products, In c. (98% a.i.) as a manufacturing use product (MUP) powder concentrat e. This product is speci fied as an additive to control mold/mildew on cement and pulp/paper (c ellulose fiber) ingredients and is used to manufacture “backerboard” (a.k.a. fiber-cement board ). The rest of the 26 registrations are enduse products (EUPs) such as soluble and flowab le concentrates, emulsifiable concentrates, wettable powder, and ready-to us e (RTU) formulations. The form ulated end-use products can range from 0.25% to 34.18% active ingredient. One of the copper-8-quinolinolate products is registered in combination with borax (B 4 

### Na 2 

### O 7 . 

### 10H 2 

### O) sodium tetraborate decahydrate (PC Code: 011102; CAS# 1303-96-4); Bifenthrin (PC Code: 128825; CAS# 82657-04-3); and Tebuconazole (PC Code: 128997; CAS# 107534-96-3).

Copper 8-quinolinolate is an algaecide, b actericide and fungicide. Other material preservation uses include in-can paint preser vation; pulp and paperboard, kraft paper; and, adhesives and glues. Copper 8-qui nolinolate is also used as a wood preservative intended for treatment of wood to be used as beams for indoor use, mushroom trays, produce picking trays/containers that may contain fruit (indirect food contact use), inte rior boat applications, wood used in greenhouse premises, equipment and containers, log homes, shingle roofs, siding, fences, decks, furniture, playground equipment, sills & baseboards, and structural building lumber.

Mode of Action of Copper-8-quinolinolate:

8-hydroxyquinoline (oxine) has been used as a disinfectant and antis eptic since 1895. It consists of two fused, 6-membered rings, one a phenol and the other a pyridine. Copper-8- quinolinolate is more toxic than either copper or 8-hydroxyquinoline alone. Mode of action of this compound: It is suggested that copper-8-qui nolinolate, which is lipid soluble, permeates cellular membranes, and then dissociates to th e half-chelate, which may then act by inserting copper at metal-binding sites on proteins and en zymes or other cellular constituents where other metals normally function. A lternatively, the 8-hydroxyquinoline can compete with coenzymes for metal-binding sites on enzymes. In both cas es, the fungicide effec tively blocks the normal metabolic processes of living cells and eith er inhibits growth or kills the fungus.

Allowable Tolerances:

### Page 4 of 17 Copper 8-quinolinolate is cleared by the U.S. Food and Drug Administration (FDA) as an indirect food additive for use as a com ponent in food packaging adhesives [21 CFR § 175.105(c)]; in paper and paperboard in contac t with aqueous and fatty foods (21 CFR § 176.170); and in paper and paperboard in contact with dry foods (21 CFR § 176.180).

Table 1. Registered Active Pro ducts of Copper 8-quinolinolate

Registration # Registration Name Company Name Formulation Type % of Active Ingredient

707-302 Cunilate 2002 Rohm & Haas Co Flowable Concentrate 10

1022-476 Chapman PQ-8 IBC Manufacturing Co Soluble Concentrate 5.4

1022-489 PQ-56 IBC Manufacturing Co Soluble Concentrate 10

1022-490 PQ-57 IBC Manufacturing Co Soluble Concentrate 5

1022-491 PQ-15 RTU Clear Wood Preservative IBC Manufacturing Co Ready-to-Use Solution 0.25

1022-492 PQ-20 IBC Manufacturing Co Soluble Concentrate 3.75

1022-493 PQ-15 IBC Manufacturing Co Soluble Concentrate 1.38

1022-503 PQ-7 IBC Manufacturing Co Soluble Concentrate 3.24

1022-504 PQ-56 RTU IBC Manufacturing Co Ready-to-Use Solution 0.8

1022-505 PQ-20 R-T-U Wood Preservative IBC Manufacturing Co Ready-to-Use Solution 0.25

1022-514 Woodguard-Oil Based Wood Preservative IBC Manufacturing Co Ready-to-Use Solution 0.675

2829-42 Socci No. 3500-WP Rohm And Haas Company Soluble Concentrate 10

2829-44 Cunilate 2174-NO Rohm And Haas Company Ready-to-Use Solution 10

2829-49 Socci No. 3500 Rohm And Haas Company Soluble Concentrate 10

2829-112 Cunilate 2419-75 Rohm And Haas Company Emulsifiable Concentrate 7.5

2829-135 Nytek 10WP Rohm And Haas Company Wettable Powder 10

### Page 5 of 17 Registration # Registration Name Company Name Formulation Type % of Active Ingredient

2829-136 Nytek 10 Rohm And Haas Company Soluble Concentrate 10

2829-137 Nytek WD Rohm And Haas Company Emulsifiable Concentrate 10

3008-91 ORD-X378 Osmose Inc. Ready-to-Use Solution 34.18

3008-93 Copper 8-Quino linolate Osmose Inc. Technical Chemical 96

10829-8 Copper 8-Quinolinolate Tanabe U.S.A. Inc. Technical Chemical 96

60061-17 Woodtreat C8 Conc entrate Kop-Coat, Inc. Soluble Concentrate 1.2

60061-18 Woodtreat C8 Ready To Use Kop-Coat, Inc. Ready-to-Use Solution 0.25

60061-22 Woodtreat C81 Ready To Use Water Repellent Fungicide Kop-Coat, Inc. Ready-to-Use Solution 1

75341-14 ORD-X240 Osmose Utilities Services, Inc. Ready-to-Use Solution .3

81819-1 Q8 Log Oil CTA Products Group Ready-to-Use Solution 0.675

83515-1 A4 James Hardie Building Products, Inc. Formulation Intermediate 98

84397-1 Moldan-10 Environmental RX, Inc. Soluble Concentrate 10

84711-1 Nylate PW Genett Group, Inc. Ready-to-Use Solution 0.625

### Page 6 of 17 Science Findings

The Agency has conducted a review of the available product chemistry, environmental fate, and ecotoxicity data for copper 8-quinolinolate. The fi ndings are summarized below.

Product Chemistry Summary

Copper 8-quinolinolate is a chelate com pound made by chemical reaction (coordinate covalent bonding) between the inorganic coppe r (II) species' and the organic moiety 8- hydroxyquinoline. The inorganic and organic parts of the molecule inherently have different chemistries and the molecule is practically non -ionizable. Its copper is bonded firmly by both primary and secondary valence linkage s, making the compound very stable.

The data submitted pertaining to the physical and chemical characteristics of the active ingredient copper 8-quinolinolate are adequate. All product chemistry data requirements have been satisfied for the active ingredient copper 8- quinolinolate. Therefore, the Agency does not anticipate needing additional product chemistry data for copper 8- quinolinolate at this time. Tables 2 and 3 contain the complete product ch emistry information for the active ingredient copper 8-quinolinolate.

Table 2. Chemical Identity of Copper 8-quinolinolate

Chemical Name Copper, bis(8-quinolinolato-N 1 

### ,O 8 

### )-

Common Names 8-Quinolin ol, copper(II) chelate Copper 8-quinolinolate Oxine-copper Synonyms Bis(8-oxyquinoline) copper Bis(8-quinolinolato)copper Bis(8-quinolinolato-N(1),O(8)) copper Copper 8-hydroxyquinoline Copper oxinate Copper oxine Copper oxyquinolate Copper quinolate Copper, bis(8-quinolinolato-N1,O8)-, Cupric 8-hydroxyquinolate Oxine-Cu Oxine-copper Trade Name Bioquin Cellu-Quin Copper-8 Cunilate 2472 Dokivin

### Table 2. Chemical Identity of Copper 8-quinolinolate

Fruitdo Milmer COX IUPAC Name bis(quinolin -8-olato-O,N)copper(II)

or cupric 8-quinolinoxide Molecular Weight 351.851g/mole
PC Code 024002 CAS Registry Number 10380-28-6 Molecular Formula C 18 

### H 12 

### Cu N 2 

### O 2 

### Registration Review Case No. Registration Review Case Name 5118 Copper salts [Copper, bis( 8-quinolinolato-N1,O8)-,] Pesticide Type Antimicrobial Conventional Chemical Classification Chemical Family Quinoline Inorganic-Copper Molecular Structure: Cu(C9H6ON)2 C 6 

### H 3 

### -C 3 

### H 3 

### -(CuN 2 

### O 2 

### )-C 6 

### H 3 

### -C 3 

### H 3



### Copper 8-quinolinolate

Page 7 of 17

### Page 8 of 17 Table 3. Product Chemistry Data Summary of 

### Copper 8-quinolinolate (TGAI 99%) Guideline No. Physical and Chemical Properties Value

830.1550 Product identity and composition Refer to Table 2.

830.1600 Description of materials used to produce the product CBI

830.1620 Description of production
process CBI

830.1650 Description of formulation
process CBI

830.1670 Discussion of formation of impurities CBI

830.1700 Preliminary analysis CBI 830.1750 Certified limits CBI 830.1800 Enforcement analytical method Electrolysis for copper determination HPLC Iodometry Gas Chromatography (GC), Infrared (IR)) Atomic Absorption Spectroscopy (AAS) for the m easurement of total copper 830.1900 Submittal of samples NA 830.6302 Color Olive green or green to yellow 830.6303 Physical State Solid Crystalline powder 830.6304 Odor Practically odorless tasteless 830.6313 Stability to sunlight, normal and elevated temperature, metals/metal ions Stable Stable towards light and U.V. It is stable at relatively high te mperatures, and is not affected by ultraviolet or other actinic rays.

Stable at ambient and elevated temperature (54°C) for 14 days.

Stable under normal conditions.

Metals/Metal Ions: Requirement no t applicable; does not come in contact with metals or metal ions. 830.6314 Oxidation/Reduction: Chemical Incompatibility No oxidizing/reducing agent

830.6315 Flammability Product does not contain flammable liquids 830.6316 Explodability Not explosive 830.6317 Storage Stability Stable 830.6319 Miscibility Not designed to mix with any petroleum solvent 830.6320 Corrosion Characteristics Non-corrosive

830.6321 Dielectric breakdown voltage Not to be used around electrical equipment

830.7000 pH 9.26 About 7 830.7050 UV/Visible absorption Maximum of absorption can be observed at the following: 410 nm, 335 nm, 320 nm, 263 nm, and 220 nm 830.7100 Viscosity Not applicable Not a liquid

### Page 9 of 17 Guideline No. Physical and Chemical Properties Value

830.7200 Melting point Decomposes above 270°C

830.7220 Boiling point 552.83 ° C (Source: EPI Suite) 830.7300 Density Density: 1.68 mg/L @ 20°C Relative density (water = 1): 1.63 g/ml; 1.687 g/cm 3 Sp Gr. 1.68 g/ml @ 20°C 830.7370 Dissociation Constant Requirement not applicable. Solubility n water - 0.07 ppm (at 25°C) No hydrolysis in water. Product does not have a disassociation constant between pH 2- 12.

830.7550 Partition coefficient ( n

octanol/water) logP = 2.46 (distilled water, 25°C) log Pow: 2.46 (Source: EPI suite)

830.7840 Solubility in water Insoluble 0.07 mg/L (pH 7, 25°C)

830.xxxx Solubility in organic solvents Slightly soluble in pyridine, quinoline, chloroform, xylene, dioxane and ethylene glycol. In methanol - 116 mg/L (25°C) In n-hexane - <0.01 mg/L (25°C) 830.7950 Vapor pressure 4.6 x 10 -8 Pascal (25°C) 6.3E-012 mm Hg (Source: EPI Suite) Henry’s law constant: 7.849 x 10 -13 atm-m3/mole (Source: EPI Suite)

N/A = Not applicable; TGAI=Technical Grade Active I ngredient; CBI= Confidential Business Information

### Page 10 of 17 Environmental Fate Summary

Copper 8-quinolinolate is a compound com posed of the inorganic (mineral) copper constituent and the organic 8-hydroxyquinoline component. Once separated by chemical or biochemical processes, these inorganic and orga nic parts of the molecule inherently have different environmental fate and chemical behavior.

The Agency has reviewed nine environmental fa te studies for copper 8-quinolinolate and the environmental fate guideline study results are summarized in Table 4 and the degradates are listed in Table 5. The Agency's conclusions for the degradation, metabolism, mobility, and leaching into soils from antisapstain trea tment of wood studies are as follows.

The chemical equilibria of copper 8-hydroxyquino line indicates the complex is a stable soluble Cu species from pH 5 to 9. Based on accep table environmental fate data, the major route of dissipation for 8-hydroxyquinoline is thr ough microbial-mediated degradation with subsequent residue incorporation into non-la bile soil organic matte r. 8-hydroxyquinoline degraded rapidly (DT 50 

### =1.74 days or DT 90 

### =47.65 days) in German an d Swiss mineral soils. The copper 8-hydroxyquinoline complex was stable to abiotic hydrolysis in pH 5, 7, and 9 buffer solutions. However, the comp lex photolyzed rapidly (DT 50 

### 0.4 to 95.7 hours) in sensitized and nonsensitized pH 7, buffer solutions. 8-hydroxyquino line and its degradates were immobile in soil column leaching studies; >88% of the radiol abeled residues were de tected in the surface 6 cm of the leaching column. These data indi cate 8-hydroxyquinoline is non-persistent and immobile in terrestrial environments. Leach ing from (sapstain use) copper 8-hydroxyquinoline treated Douglas fir wood is 666 mg/1000 board feet while for Hemlock it is 229 mg/1000 board feet after 8 stimulated rain cycles. The estimated log K ow 

### for copper 8-quinolinolate is 2.5, which indicates that it is not likely to bioaccumulat e/bioconcentrate in aqua tic organisms like fish.

Table 4. Environmental Fate Data Summary for Copper 8-Quinolinolate

OPP Guideline Fate Studies Half-Life Degradates Study Status

161-1 Hydrolysis pH 5 pH 7 pH 9 > 30 days in each pH (Stable)

Half life at pH 5 was extrapolated to 630 days.

Copper 8-hydroxyquinoline is stable in pH 5, 7, and 9 buffer solutions. No major degradates. Acceptable

MRID 42899001

161-2 Aqueous Photolysis DT 50 - 0.4 hours (in sensitized pH 7 buffer solution)

DT 50 - 60.4 to 95.7 hours (in non-sensitized pH 7 buffer solution) Major photodegradate Dihydroxyquinoline Acceptable

MRID 42895501

### Page 11 of 17 OPP Guideline Fate Studies Half-Life Degradates Study Status

Photolytically copper-8 quinolinate is unstable.

Aerobic Soil Metabolism (European soils: The US equivalents are loamy sand and sandy loam) Uncomplexed oxine (8- hydroxyquinoline) was not persistent (DT 50 = 1.74 days or DT 90 = 47.65 days) in German and Swiss soils. The major route of 8- hydroxyquinoline (oxine) dissipation was controlled through microbial-mediated mineralization with residueincorporation into nonlabile soil organic matter (fulvic and humic acids). There were many metabolites detected but the major one which was identified is the free ligand. Acceptable

MRID 42895502 162-1

Aerobic Soil Metabolism (Japanese soils equivalent to light loam and the other loam) This study was conducted for 16 weeks. The study indicate that in aerobic soils, the active does metabolizes and microbial decomposition does take place but up to maximum of 15% only by the end of week 16. Copper-8 quinolinate, therefore is relatively stable in aerobic soils. A number of metabolites were detected but except for the identification of the free ligand, no other metabolites were identified. Supplemental

MRID 43677301

163-1 Unaged and Aged Soil Column Leaching Two studies were conducted on non-aged soil samples of the active and on the aged soil samples of the active. Both studies were run with 4 European soils which were equivalent US soils: sandy loam, loamy sand, sand and loamy sand. For samples in non-aged soils, 90% of the active was detected on the top 0-6 cm soil columns. For aged soils, 87.9% of the active stayed in the top 0-6 cm soil columns. Uncomplexed oxine (8- hydroxyquinoline) is not mobile in soils and will stay Aged oxine degradates wereidentified as oxine, nonextractable soil organic matter (fulvic and humic acids), and CO 2 . Acceptable

MRID 42895503 MRID 42895504

### Page 12 of 17 OPP Guideline Fate Studies Half-Life Degradates Study Status

on the soil surfaces and is not likely to migrate out into ground water.



### Canadian Standard Leaching Test for Antisapstain Chemicals

Leaching of copper 8- quinolinolate antisapstain from Hemlock and Douglas Fir It leaches out from the wo od surfaces when treated for antisapstain use. For Hemlock and Douglas Fir treated wood, leaching increases for the first four days and attains the minimum level after 8 days. A steady decline in concentration occurred from fourth to th e eighth cycle. Rates of leaching is dependent on the concentration and retention of th e active on the wood surfaces.

The total amount of copper 8-quinolinolate leached from treated lumbers (eight leaching cycles) were 660 mg/thousan d board feet of Hemlock and 229 mg/thousand board feet of Douglas fir.

In both studies the leaching rates increased for the first four rainfall cycles and declined thereafter. Supplemental

MRID 43620602 MRID 43620603

Leaching of copper 8- quinolinolate from Lumber Spray treated with Maag Sapstain This was a preparative study conducted with the objective of determining the quantity of copper oxine which leaches fro m: 1)freshly cut w ood; 2) planed; 3) rough sawn hem-fir wood. The leaching was triggered with the simulated rainfall. The wood was treated with Nytek GD (oxine copper) and Nytek GD attached to a sticker. The spray methods were: a) conventional spray system and b) oil based formulation applied with electrostatic equipment. The simulated rainfall rate was 2.7 mm/hour and was triggered from a sprinkler system. Wood samples were treated with the rain fall two hours after the treatment as well as 24 hours after the treatment. Results: 1) Leaching was observed with both types of treated wood: Nytek GD and Nytek GD with a sticker. 2) Amounts of leachates declined with time; 3)More copper oxine leached from rough edged w ood than with plan ed wood when the wood was treated with the conventional sp ray system; 4) leaching from 24 hour delayed rainfall treatement was 7% and leaching from 2 hour delayed rainfall treatment samples was 12% from rough edge wood samples. Pl aned wood showed no appreciable difference between the tow types. Delayed samples leached less; 5) Attachment of sticker did not result in any difference in leaching; 6) wood samples treated with electrostatic spray showed less leaching, but the application rate with this method of application wa s less than the conventional method of
spray. Supplemental

MRID 43637001

### Table 5. Degradates of Copper 8-quinolinolate

8-Hydroxyquinoline

(Uncomplexed oxine)

Mol Wt 145.1607 C9H6NOH Photoproduct :



### Water Quality

Copper 8-quinolinolate is not identified as a cause of impairment for any water bodies listed as impaired under section 303(d) of the Clean Water Act, based on information provided at http://iaspub.epa.gov/tmdl_waters10/attains_ nation_cy.cause_detail_303d? p_cause_group_id=88 5 . In addition, no Total Maximum Daily Load s (TMDL) have been developed for copper 8- quinolinolate, based on information provided at http://iaspub.epa.gov/tmdl_waters10/attains_ nation.tm dl_pollutant_de tail?p_pollutant_group_id =885&p_pollutant_group_name=PESTICIDES . More information on impaired water bodies and TMDLs can be found at http://www.epa.gov/owow/tmdl/ . The Agency invites submission of water quality data for this pesticide. To the extent possible, data should conform to the quality standards in Appendix A of the OPP Standard Operating Procedur e: Inclusion of Impaired Water Body and Other Water Quality Data in OPP’s Regi stration Review Risk Assessment and Management Process (see: http://www.epa.gov/oppfead1/cb/ppdc/2006/novem ber06/session1- sop.pdf ), in order to ensure they can be used qua ntitatively or qualitatively in pesticide risk
assessments.

Page 13 of 17

### Page 14 of 17 Ecological Effects Summary

The Agency has reviewed twenty two ecotoxicology studies for copper 8-quinolinolate. The twenty two studies submitted on the 10 - 100% active ingredient were all found to be acceptable for use in a hazard assessment. Ecot oxicity guideline studies and the results are summarized in Table 6. The Agency's conclusions for the avian, aquatic effects and plant studies are as follows.

Toxicity to Birds Available data indicate that copper 8-quinolinolat e is slightly toxic to practically nontoxic to birds on an acute oral basi s and sub-acute dietary basis.

Toxicity to Aquatic Animals On an acute basis, copper 8-quino linolate is highly to very highly toxic to freshwater fish; very highly toxic to freshwater invertebrates; and very highly toxic to estuarine/marine invertebrates.

Toxicity to Plants Algal toxicity data indicate that copper 8-qui nolinolate is detrimenta l at less than 1.0 ppm to green alga and marine diatom. Therefore, ad ditional Tier II (dose re sponse) studies listed on page 18 of this document are required.

Table 6. Ecotoxicity Data Summary for Copper 8-quinolinolate

GUIDE LINE COMMON NAME TAXONOMIC NAME TEST TYPE % AI STUDY TIME DOSE TYPE TOXI CITY TOX LEVEL NOEL STUDY STATUS TOX CATEGORY Avian Acute Oral Toxicity
850.2100

71-1 Bobwhite quail Colinus virginianus O 99.5 14 D LD 50 618 MGK N.D. Core Slightly Toxic
850.2100

71-1 Mallard duck Anas platyrhynchos O 99.5 14 D LD 50 >2000 MGK 2000 Core Practically Non-toxic Avian Subacute Dietary Toxicity
850.2200

71-2a Bobwhite quail Colinus virginianus D 99.5 8 D LC 50 3248 PPM 1300 Core Slightly Toxic
850.2200

71-2b Mallard duck Anas platyrhynchos D 99.5 8 D LC 50 >5200 PPM 2600 Core Practically Non-toxic
850.2200

71-2a Bobwhite quail Colinus virginianus D 10 8 D LC 50 3934 PPM N.R. Core Slightly Toxic
850.2200

71-2b Mallard duck Anas platyrhynchos D 10 8 D LC 50 >10000 PPM 2419 Core Practically Non-toxic Freshwater Fish Acute Toxicity
850.1075

72-1a Bluegill sunfish Lepomis macrochirus F 100 96 hr LC 50 21.6 PPB 10.8 Core Very Highly toxic
850.1075

72-1a Bluegill sunfish Lepomis macrochirus S 90 96 hr LC 50 290 PPB N.R. Suppl Highly toxic

850.1075

72-1a Bluegill sunfish Lepomis macrochirus S 10 96 hr LC 50 180 PPB N.R. Core Very Highly toxic
850.1075 Rainbow Oncorhynchus F 100 96 hr LC 50 8.9 PPB 6.2 Core Very Highly

### Page 15 of 17 GUIDE LINE COMMON NAME TAXONOMIC NAME TEST TYPE % AI STUDY TIME DOSE TYPE TOXI CITY TOX LEVEL NOEL STUDY STATUS TOX CATEGORY
72-1c trout mykiss toxic
850.1075

72-1c Rainbow
trout Oncorhynchus mykiss SR 80 96 hr LC 50 9.68 PPB 7.11 Core Very Highly toxic
850.1075

72-1c Rainbow
trout Oncorhynchus mykiss S 10 96 hr LC 50 110 PPB N.R. Core Very Highly toxic
850.1075

72-1a Coho salmon Oncorhynchus kisutch F 100 96 hr LC 50 13.9 PPB 6.6 Core Very Highly toxic Freshwater Fish Early Life-Stage (Chronic)Toxicity
850.1400

72-4a Rainbow
trout Oncorhynchus mykiss S 100 95 D LOEC

MATC 3.51

Survive:

3.51

Growth:

3.51

>1.69 <3.51 PPB 1.69 Suppl Chronic toxic effects

Fish Life Cycle Toxicity
850.1500

72-5 Fathead minnow Pimephales promelas F 98 164 D LOEC 6.04 PPB 2.99 Core Chronic toxic effects Freshwater Invertebrate Acute Toxicity
850.1010

72-2 Water flea Daphnia
magna F 98 48 hr EC 50 LOEC 162 36 PPB <36 Core Very Highly toxic
Freshwater Invertebrate Life -Cycle (Chronic) Toxicity
850.1300

72-4b Water flea Daphnia
magna F 100 21 D LOEC Egg Hatch: Survive: Growth: MATC: 10.4

35.1 70.9 10.4 >6.4 <10.4 PPB 6.4 Suppl Chronic toxic effects

Estuarine/Marine Mysid Acute Toxicity - Invertebrates
850.1035

72-3c Mysid shrimp Americamysis bahia F 100 96 hr LC 50 49.7 PPB 6.8 Suppl Very Highly toxic Oyster Acute Toxicity Test (She ll Deposition) - Invertebrates
850.1055

72-3b Eastern oyster Embryos & Larvae Crassostrea virginica F 100 48 hr EC 50 36.3 PPB 11.1 Core Very Highly toxic

Algal Toxicity (Aquatic (Algal) Plant Growth Tier II)
850.5400

123-2 Green algae Dunaliella tertiolecta S 100 5 D EC 50 LOEC 14.3 15.4 PPB 9.0 Core Detrimental effects
850.5400

123-2 Marine diatom Nitzschia punctata S 100 5 D EC 50 LOEC 7.3 7.2 PPB N.D. Suppl Detrimental effects
850.5400

123-2 Marine diatom Skeletonema

costatum S 98 5 D EC 50 1.9 PPB <0.7 Core Detrimental effects

### Page 16 of 17 Ecotoxicity data are adequate to provide the Agency with sufficient information to determine appropriate label precautions.

Adverse Ecological Incidents

There are no reported incidents in the Agency ’s Ecological Incident Information System (EIIS) regarding copper 8-quinolinolate (P C Code 024002). The absence of documented incidents does not necessarily mean that such incidents did not occur.

Ecological Effects Risk Assessment

The Agency has not conducted a risk asse ssment that supports a complete endangered species determination. The ecological risk as sessment planned during registration review will allow the Agency to determine whether copper 8-quinolinolate’s use has “no effect or “may affect” federally listed threatened or endangered species (listed sp ecies) or their designed critical habitats. When an assessment concludes that a pe sticide’s use “may affect” a listed species or its designated critical habitat, the Agency will c onsult with the U.S. Fish and Wildlife Service and/or National Marine Fi sheries Services (the Se rvices), as appropriate.

### Page 17 of 17 Environmental Fate and Ecotoxicity Data Gaps and Anticipated Work

None.