13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J11120369

Lab Contact: Jason C Perkins

Date: 1/6/2012

Customer Address: 253 Plant Allen Road

Customer Name(s): BILL KENNEDY, RON LAWS, ALLEN STOWE, ROBBIN JOLLY, DON SCRUG

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis,

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Analytical Lab 

Page 1 of 32
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2011027622 ALLEN 20-Dec-11 9:19 AM JBW FGD Purge Eff 

2011027623 ALLEN 20-Dec-11 8:47 AM JBW BioReactor 1 Inf 

2011027624 ALLEN 20-Dec-11 10:09 AM JBW BioReactor 1 Inf BLANK 

2011027625 ALLEN 20-Dec-11 8:52 AM JBW BioReactor 2 Eff 

2011027626 ALLEN 20-Dec-11 10:11 AM JBW BioReactor 2 Eff BLANK 

2011027627 ALLEN 20-Dec-11 2:45 PM JBW Filter Blk 

6 Total Samples

Analytical Lab 

Page 2 of 32
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

The Vendor Laboratories have been qualified by the 

Analytical Laboratory

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: Mary Ann Ogle Date: 1/6/2012

Yes

Electronic Data Deliverable (EDD) Sent Separately

Analytical Lab 

Page 3 of 32
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11120369

2011027622

Collection Date: 20-Dec-11 9:19 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

ALKALINITY (FIXED END POINT 4.5)

Vendor Parameter Complete V_PRISM

Carbonate, Bicarbonate, and Hydroxide Alkalinity

Carbonate (CO3) Complete V_PRISM

Hydroxide (OH) Complete V_PRISM

Bicarbonate (HCO3) Complete V_PRISM

NITRITE + NITRATE (COLORIMETRIC)

Nitrite + Nitrate (Colorimetric) 100 mg-N/L EPA 353.2 03-Jan-12 13:26 BGN9034 1.0

INORGANIC IONS BY IC

Bromide 28 mg/L EPA 300.0 29-Dec-11 17:55 JAHERMA 5

Chloride 2300 mg/L EPA 300.0 29-Dec-11 17:55 JAHERMA 50

Sulfate 2400 mg/L EPA 300.0 29-Dec-11 17:55 JAHERMA 50

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 104 ug/L EPA 245.1 30-Dec-11 09:01 AGIBBS 2.5

Mercury Dissolved (cold vapor) in Water (Filtered)

Mercury (Hg) 60.8 ug/L EPA 245.1 30-Dec-11 11:35 AGIBBS 2.5

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 214 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.5

Calcium (Ca) 2260 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.10

Cobalt (Co) 0.087 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Lithium (Li) 0.342 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Magnesium (Mg) 1210 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Potassium (K) 74.3 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 1.0

Sodium (Na) 47.9 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.5

DISSOLVED METALS BY ICP-MS

Selenium (Se) 1700 ug/L EPA 200.8 27-Dec-11 11:09 MHH7131 10

Analytical Lab 

Page 4 of 32
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11120369

2011027622

Collection Date: 20-Dec-11 9:19 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

TOTAL RECOVERABLE METALS BY ICP-MS

Antimony (Sb) < 10.0 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Arsenic (As) 203 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Beryllium (Be) 12.1 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Cadmium (Cd) < 10.0 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Chromium (Cr) 245 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Copper (Cu) 182 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Lead (Pb) 157 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Nickel (Ni) 295 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Selenium (Se) 1770 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Silver (Ag) < 10.0 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 10

Zinc (Zn) 270 ug/L EPA 200.8 04-Jan-12 12:08 KRICHAR 20

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

TOTAL DISSOLVED SOLIDS

TDS 9000 mg/L SM2540C 28-Dec-11 13:41 TJA7067 10

TOTAL SUSPENDED SOLIDS

TSS 3600 mg/L SM2540D 22-Dec-11 11:30 TJA7067 5

2011027623

Collection Date: 20-Dec-11 8:47 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

ALKALINITY (FIXED END POINT 4.5)

Vendor Parameter Complete V_PRISM

Carbonate, Bicarbonate, and Hydroxide Alkalinity

Bicarbonate (HCO3) Complete V_PRISM

Hydroxide (OH) Complete V_PRISM

Carbonate (CO3) Complete V_PRISM

NITRITE + NITRATE (COLORIMETRIC)

Nitrite + Nitrate (Colorimetric) 97 mg-N/L EPA 353.2 03-Jan-12 13:28 BGN9034 1.0

INORGANIC IONS BY IC

Bromide 40 mg/L EPA 300.0 29-Dec-11 18:11 JAHERMA 5

Chloride 2900 mg/L EPA 300.0 29-Dec-11 18:11 JAHERMA 50

Sulfate 2300 mg/L EPA 300.0 29-Dec-11 18:11 JAHERMA 50

Analytical Lab 

Page 5 of 32
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11120369

2011027623

Collection Date: 20-Dec-11 8:47 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 66.1 ug/L EPA 245.1 30-Dec-11 09:04 AGIBBS 2.5

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 201 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.5

Calcium (Ca) 1550 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.1

Cobalt (Co) < 0.050 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Lithium (Li) 0.131 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Magnesium (Mg) 1060 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Potassium (K) 28.7 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 1.0

Sodium (Na) 47.5 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50

TOTAL RECOVERABLE METALS BY ICP-MS

Antimony (Sb) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Arsenic (As) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Beryllium (Be) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Cadmium (Cd) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Chromium (Cr) 39.7 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Copper (Cu) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Lead (Pb) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Nickel (Ni) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Selenium (Se) 1780 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Silver (Ag) < 10.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 10

Zinc (Zn) < 20.0 ug/L EPA 200.8 04-Jan-12 12:11 KRICHAR 20

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011027624

Collection Date: 20-Dec-11 10:09 AM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

Analytical Lab 

Page 6 of 32
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11120369

2011027625

Collection Date: 20-Dec-11 8:52 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

ALKALINITY (FIXED END POINT 4.5)

Vendor Parameter Complete V_PRISM

Carbonate, Bicarbonate, and Hydroxide Alkalinity

Bicarbonate (HCO3) Complete V_PRISM

Hydroxide (OH) Complete V_PRISM

Carbonate (CO3) Complete V_PRISM

NITRITE + NITRATE (COLORIMETRIC)

Nitrite + Nitrate (Colorimetric) 1.00 mg-N/L EPA 353.2 03-Jan-12 13:39 BGN9034 0.01

INORGANIC IONS BY IC

Bromide 37 mg/L EPA 300.0 29-Dec-11 18:26 JAHERMA 5

Chloride 2900 mg/L EPA 300.0 29-Dec-11 18:26 JAHERMA 50

Sulfate 2800 mg/L EPA 300.0 29-Dec-11 18:26 JAHERMA 50

MERCURY 1631

Vendor Parameter Complete V_BRAND

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) < 1.00 ug/L EPA 245.1 30-Dec-11 09:06 AGIBBS 1.0

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 213 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50

Calcium (Ca) 1560 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.10

Cobalt (Co) < 0.050 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Lithium (Li) 0.165 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Magnesium (Mg) 1220 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.050

Potassium (K) 68.4 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 1.0

Sodium (Na) 55.9 mg/L EPA 200.7 29-Dec-11 15:05 SLS2560 0.50

TOTAL RECOVERABLE METALS BY ICP-MS

Antimony (Sb) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Arsenic (As) 7.04 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Beryllium (Be) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Cadmium (Cd) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Chromium (Cr) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Copper (Cu) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Lead (Pb) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Nickel (Ni) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Selenium (Se) 14.5 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Silver (Ag) < 5.00 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 5

Zinc (Zn) < 10.0 ug/L EPA 200.8 04-Jan-12 12:14 KRICHAR 10

Analytical Lab 

Page 7 of 32
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J11120369

2011027625

Collection Date: 20-Dec-11 8:52 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

SELENIUM SPECIATION

Vendor Parameter Complete V_AS&C

2011027626

Collection Date: 20-Dec-11 10:11 AM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

MERCURY 1631

Vendor Parameter Complete V_BRAND

2011027627

Collection Date: 20-Dec-11 2:45 PM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

Analyst

DISSOLVED METALS BY ICP-MS

Selenium (Se) 2.33 ug/L EPA 200.8 27-Dec-11 11:09 MHH7131 1

Analytical Lab 

Page 8 of 32
 

January 3, 2012 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: HAPS/MACT Testing Allen (LIMS # J11120369) 

Dear Mr. Perkins, 

Attached is the report associated with three (3) aqueous samples submitted for selenium 

speciation analysis on December 22, 2011. The samples were received in a sealed cooler at -

0.3°C on December 23, 2011. Selenium speciation analysis was performed via ion 

chromatography inductively coupled plasma dynamic reaction cell mass spectrometry (IC-ICPDRC-MS). Any issues associated with the analysis are addressed in the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

 Vice President 

 Applied Speciation and Consulting, LLC 

 

Analytical Lab 

Page 9 of 32
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: HAPS/MACT Testing Allen (LIMS # J11120369) 

January 3, 2012 

1. Sample Reception 

Three (3) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on December 22, 2011. The 

samples were received on December 23, 2011 in a sealed container at -0.3°C. 

The samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and was designated a discrete sample identifier. An aliquot of each 

sample was filtered (0.45μm) and each filtrate was stored in a secure, monitored cryofreezer 

(maintained at a temperature of -80oC) until selenium speciation analysis could be performed 

via ion chromatography inductively coupled plasma dynamic reaction cell mass spectrometry 

(IC-ICP-DRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Selenium Speciation Analysis by IC-ICP-DRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed 

autosampler vial. No further sample preparation was performed as any chemical alteration of 

a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

Analytical Lab 

Page 10 of 32
each analytical day. All calibration curves, associated with each species of interest, are 

standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval 

of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-DRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma dynamic reaction 

cell mass spectrometry (IC-ICP-DRC-MS) on December 29, 2011. An aliquot of each

sample is injected onto an anion exchange column and mobilized by a basic (pH > 7) 

gradient. The eluting selenium species are then introduced into a radio frequency (RF) 

plasma where energy-transfer processes cause desolvation, atomization, and ionization. The 

ions are extracted from the plasma through a differentially-pumped vacuum interface and 

travel through a pressurized chamber (DRC) containing a specific reactive gas which 

preferentially reacts with interfering ions of the same target mass to charge ratios (m/z). A 

solid-state detector detects ions transmitted through the mass analyzer and the resulting 

current is processed by a data handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

quality control parameters associated with this sample were within acceptance limits.

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

Analytical Lab 

Page 11 of 32
If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Analytical Lab 

Page 12 of 32
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff ND (<3.0) 1430 ND (<5.9) ND (<4.0) ND (<4.0) 0 (0)

BioReactor 1 Inf 4.05 1400 ND (<1.5) 1.6 ND (<1.0) 0 (0)

BioReactor 2 Eff 7.28 3.50 ND (<1.5) ND (<1.0) ND (<1.0) 0 (0)

All results reflect the applied dilution and are reported in μg/L

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

n = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: HAPS/MACT Testing Allen

Contact: Jay Perkins

Date: January 3, 2012

LIMS #J11120369

Analytical Lab 

Page 13 of 32
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 50x eMDL 200x

Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.015 0.74 3.0

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.016 0.79 3.2

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.030 1.5 5.9

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.020 1.0 4.0

SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.020 1.0 4.0

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Se(IV) LCS 9.57 9.72 101.5

Se(VI) LCS 9.48 9.00 94.9

SeCN LCS 8.92 9.09 101.9

MeSe(IV) LCS 6.47 6.17 95.3

SeMe LCS 9.32 9.14 98.1

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: HAPS/MACT Testing Allen

Contact: Jay Perkins

LIMS #J11120369

Date: January 3, 2012

Report Generated by: Russell Gerads

Analytical Lab 

Page 14 of 32
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Se(IV) Batch QC ND (<3.0) ND (<3.0) NC NC

Se(VI) Batch QC 1654 1617 1636 2.2

SeCN Batch QC ND (<5.9) ND (<5.9) NC NC

MeSe(IV) Batch QC ND (<4.0) ND (<4.0) NC NC

SeMe Batch QC ND (<4.0) ND (<4.0) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Se(IV) Batch QC 1112 1166 104.8 1112 1185 106.6 1.7

Se(VI) Batch QC 1009 2584 94.0 1009 2600 95.6 0.6

SeCN Batch QC 915.0 829.1 90.6 915.0 812.3 88.8 2.0

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: HAPS/MACT Testing Allen

Contact: Jay Perkins

LIMS #J11120369

Date: January 3, 2012

Report Generated by: Russell Gerads

Analytical Lab 

Page 15 of 32
Analytical Lab 

Page 16 of 32
 

3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

January 3, 2012 

Duke Energy 

ATTN: Jay Perkins 

Scientific Support-Laboratory

13339 Hagers Ferry Road 

Huntersville NC 28078 

jcperkins@duke-energy.com 

labcustomer@duke-energy.com 

RE: Project DUK-HV1101 Client Project: J11120369 

Dear Mr. Perkins, 

On December 23, 2011, Brooks Rand Labs (BRL) received two (2) waste water samples and 

two (2) corresponding field blanks. Samples were logged-in for total mercury (Hg) analysis. All 

samples were received, prepared, analyzed, and stored according to BRL SOPs and EPA 

methodology. 

The results were blank-corrected as described in the calculations section of the applicable 

SOP(s) and may be evaluated using adjusted reporting limits to account for sample aliquot size. 

Please refer to the Sample Results page for sample-specific detection limits and other details. 

Aside from concentration qualifiers, all data was reported without further qualification and all 

associated quality control sample results met the acceptance criteria. 

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report. 

Please feel free to contact me if you have any questions regarding this report. 

Sincerely, 

 

Tiffany Stilwater 

Project Manager 

tiffany@brooksrand.com 

Analytical Lab 

Page 17 of 32
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand, Ltd., those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Laboratory Data Validation Functional Guidelines for Evaluating Inorganic 

Analyses; USEPA; July 2002. These supersede all previous qualifiers ever employed by BRL.

B Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H Holding time and/or preservation requirements not met. Result is estimated.

J Estimated value. A full explanation is presented in the narrative.

J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N Spike recovery was not within acceptance criteria. Result is estimated.

R Rejected, unusable value. A full explanation is presented in the narrative.

U Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 18 of 32
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1152037-01 Influent Sample 12/20/2011 12/23/2011

BioReactor 1 Inf Hg Blk 1152037-02 DIW Field Blank 12/20/2011 12/23/2011

BioReactor 2 Eff 1152037-03 Effluent Sample 12/20/2011 12/23/2011

BioReactor 2 Eff Hg Blk 1152037-04 DIW Field Blank 12/20/2011 12/23/2011

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 12/28/2011 01/02/2012 B112204 1200001

Sample Results

Sample Analyte Report Matrix Fraction Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1152037-01 Hg Influent T 72600 303 808 ng/L B112204 1200001

BioReactor 1 Inf Hg Blk

1152037-02 Hg DIW T 0.15 U 0.15 0.40 ng/L B112204 1200001

BioReactor 2 Eff

1152037-03 Hg Effluent T 7.10 0.73 1.95 ng/L B112204 1200001

BioReactor 2 Eff Hg Blk

1152037-04 Hg DIW T 0.15 U 0.15 0.40 ng/L B112204 1200001

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Analytical Lab 

Page 19 of 32
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Batch: B112204

Analyte Native Spike Result Units REC & Limits RPD & Limits

Method: EPA 1631

Lab Matrix: Water

Sample

B112204-SRM1 Certified Reference Material (1153040, NIST 1641d 1000x dilution)

Hg 1568 1675 ng/L 107% 85-115

B112204-MS2 Matrix Spike (1152038-09)

Hg 115.5 194.9 323.0 ng/L 106% 71-125

B112204-MSD2 Matrix Spike Duplicate (1152038-09)

Hg 115.5 199.3 326.6 ng/L 106% 71-125 241%

Method Blanks & Reporting Limits

Batch: B112204

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B112204-BLK1 0.03 ng/L

B112204-BLK2 0.06 ng/L

B112204-BLK3 0.06 ng/L

B112204-BLK4 0.03 ng/L

Average: 0.05 Standard Deviation: 0.02 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.40

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Analytical Lab 

Page 20 of 32
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1200001

Instrument: THG-05 Method: EPA 1631

Date: 01/02/2012

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1200001-IBL1 5.97 pg of Hg

1200001-IBL2 10.23 pg of Hg

1200001-IBL3 5.33 pg of Hg

1200001-IBL4 5.20 pg of Hg

1200001-CAL1 25.00 24.46 pg of Hg 98%

1200001-CAL2 100.0 102.3 pg of Hg 102%

1200001-CAL3 500.0 476.6 pg of Hg 95%

1200001-CAL4 2500 2587 pg of Hg 103%

1200001-CAL5 10000 10160 pg of Hg 102%

1200001-ICV1 1568 1675 pg of Hg 107% 85-115

1200001-CCB1 10.1 pg of Hg

1200001-CCV1 500.0 510.9 pg of Hg 102% 77-123

1200001-CCV2 500.0 471.0 pg of Hg 94% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Analytical Lab 

Page 21 of 32
Project ID: DUK-HV1101

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1152037-01 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 12/23/2011

Collected: 12/20/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250 mL none n/a Cooler

Lab ID: 1152037-02 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 12/23/2011

Collected: 12/20/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250 mL none n/a Cooler

Lab ID: 1152037-03 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 12/23/2011

Collected: 12/20/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250 mL none n/a Cooler

Lab ID: 1152037-04 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 12/23/2011

Collected: 12/20/2011

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71470160

10

Bottle FLPE Hg-T 250 mL none n/a Cooler

Shipping Containers

Cooler

Tracking No: 4726 7966 7029 via FedEx

Temperature: ambient

Coolant Type: None

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: December 23, 2011 9:30

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Analytical Lab 

Page 22 of 32
Analytical Lab 

Page 23 of 32
This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample 

Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed 

according to the referenced methods. 

Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case 

narrative.

NC Certification No. 402

SC Certification No. 99012

NC Drinking Water Cert No. 37735

Case Narrative

12/30/2011

Duke Energy Corporation (04)

Jay Perkins

13339 Hagers Ferry Road

Huntersville, NC 28078

Project: HAPS/MACT Testing Allen

Project No.: J11120369

Lab Submittal Date: 12/22/2011

Prism Work Order: 1120633

Please call if you have any questions relating to this analytical report.

Respectfully,

PRISM LABORATORIES, INC.

VP Laboratory Services Reviewed By

Data Qualifiers Key Reference:

HT Sample received and analyzed outside of the hold time.

MDL Method Detection Limit

BRL Below Reporting Limit

RPD Relative Percent Difference

*

Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and 

reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 1 of 8

Analytical Lab 

Page 24 of 32
Sample Receipt Summary

12/30/2011

Prism Work Order: 1120633

Client Sample ID Lab Sample ID Matrix Date Sampled Date Received

2011027622/FGD Purge Eff 1120633-01 Water 12/20/11 12/22/11

2011027623/BioReactor 1 Inf 1120633-02 Water 12/20/11 12/22/11

2011027625/BioReactor 2 Eff 1120633-03 Water 12/20/11 12/22/11

Samples received in good condition at 0.3 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 2 of 8

Analytical Lab 

Page 25 of 32
Laboratory Report

12/30/2011

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: HAPS/MACT Testing Allen

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 1120633-01

Prism Work Order: 1120633

Time Collected: 12/20/11 09:19

Time Submitted: 12/22/11 17:15

2011027622/FGD Purge Eff

J11120369

General Chemistry Parameters

pH 7.2 HT pH Units 1 *SM4500-H B 12/23/11 10:30 JAB P1L0549

Total Alkalinity 67 mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0592

Carbonate Alkalinity BRL mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0593

Bicarbonate Alkalinity 67 mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0594

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 3 of 8

Analytical Lab 

Page 26 of 32
Laboratory Report

12/30/2011

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: HAPS/MACT Testing Allen

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 1120633-02

Prism Work Order: 1120633

Time Collected: 12/20/11 08:47

Time Submitted: 12/22/11 17:15

2011027623/BioReactor 1 Inf

J11120369

General Chemistry Parameters

pH 7.7 HT pH Units 1 *SM4500-H B 12/23/11 10:30 JAB P1L0549

Total Alkalinity 110 mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0592

Carbonate Alkalinity BRL mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0593

Bicarbonate Alkalinity 110 mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0594

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 4 of 8

Analytical Lab 

Page 27 of 32
Laboratory Report

12/30/2011

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: HAPS/MACT Testing Allen

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 1120633-03

Prism Work Order: 1120633

Time Collected: 12/20/11 08:52

Time Submitted: 12/22/11 17:15

2011027625/BioReactor 2 Eff

J11120369

General Chemistry Parameters

pH 7.7 HT pH Units 1 *SM4500-H B 12/23/11 10:30 JAB P1L0549

Total Alkalinity 420 mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0592

Carbonate Alkalinity BRL mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0593

Bicarbonate Alkalinity 420 mg/L 5.0 1.4 1 *SM2320 B 12/28/11 10:45 JAB P1L0594

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 5 of 8

Analytical Lab 

Page 28 of 32
12/30/11

Level II QC Report

13339 Hagers Ferry Road Project No: J11120369

Duke Energy Corporation (04) Project: HAPS/MACT Testing Allen

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 1120633

Time Submitted: 12/22/2011 5:15:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P1L0549 - NO PREP

LCS (P1L0549-BS1) Prepared & Analyzed: 12/23/11 

pH 6.85 pH Units 6.860 100 99-101

Batch P1L0592 - NO PREP

Blank (P1L0592-BLK1) Prepared & Analyzed: 12/28/11 

Total Alkalinity BRL 5.0 mg/L

LCS (P1L0592-BS1) Prepared & Analyzed: 12/28/11 

Total Alkalinity 252 5.0 mg/L 250.0 101 90-110

LCS Dup (P1L0592-BSD1) Prepared & Analyzed: 12/28/11 

Total Alkalinity 256 5.0 mg/L 250.0 102 90-110 1 200

Batch P1L0593 - NO PREP

Blank (P1L0593-BLK1) Prepared & Analyzed: 12/28/11 

Carbonate Alkalinity BRL 5.0 mg/L

LCS (P1L0593-BS1) Prepared & Analyzed: 12/28/11 

Carbonate Alkalinity 252 5.0 mg/L 90-110

LCS Dup (P1L0593-BSD1) Prepared & Analyzed: 12/28/11 

Carbonate Alkalinity 256 5.0 mg/L 90-110 1 200

Batch P1L0594 - NO PREP

Blank (P1L0594-BLK1) Prepared & Analyzed: 12/28/11 

Bicarbonate Alkalinity BRL 5.0 mg/L

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 6 of 8

Analytical Lab 

Page 29 of 32
12/30/11

Level II QC Report

13339 Hagers Ferry Road Project No: J11120369

Duke Energy Corporation (04) Project: HAPS/MACT Testing Allen

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 1120633

Time Submitted: 12/22/2011 5:15:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P1L0594 - NO PREP

LCS (P1L0594-BS1) Prepared & Analyzed: 12/28/11 

Bicarbonate Alkalinity 252 5.0 mg/L 250.0 101 90-110

LCS Dup (P1L0594-BSD1) Prepared & Analyzed: 12/28/11 

Bicarbonate Alkalinity 256 5.0 mg/L 250.0 102 90-110 1 200

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 7 of 8

Analytical Lab 

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Analytical Lab 

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