Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 1 of 20

Data Requirement: PMRA Data Code: 9.8.4 (TGAI) or 9.8.6 (EP)

EPA DP Barcode: 416757

OECD Data Point: IIA 8.12 (TGAI) and IIIA 10.8.1.1 (EP)

EPA Guideline: 850.4150

Test material: Nicosulfuron 75 WG Purity: 75.1%

Common name:

Chemical name: IUPAC: 

CAS name: 2-[[[[(4,6-Dimethoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]-N,N-dimethyl-3-

pyridinecarboxamide

CAS No.: 111991-09-4

Synonyms: DPX-V9360 75WG, DPX-V9360-119, Nicosulfuron (DPX-V9360) 75WG

Primary Reviewer: Teresa Nelis Signature: 

Senior Scientist, CDM Smith Date: 12/13/14

Secondary Reviewer: Teri S. Myers Signature: 

Senior Scientist, CDM Smith Date: 04/03/15 

Primary Reviewer: Hannah Yingling Date: 10/14/2015

Biologist, EPA

CITATION: Aufderheide, J.A. 2004. Nicosulfuron (DPX-V9360) 75WG plus Isodecylalcohol Ethoxylated (IN-KG691) 

Surfactant: A Greenhouse Study to Investigate the Effects on Vegetative Vigor of Ten Terrestrial Plant Species 

Following Foliar Exposure. Unpublished study performed by ABC Laboratories, Inc., Columbia, MO. Performing 

Laboratory Study Number: 48283. Study sponsored by E.I. du Pont de Nemours and Company, Wilmington, 

Delaware. Study completed November 15, 2004.

DISCLAIMER: This document provides guidance for EPA and PMRA reviewers on how to complete a data 

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to terrestrial vascular 

plants. It is not intended to prescribe conditions to any external party for conducting this study nor to establish 

absolute criteria regarding the assessment of whether the study is scientifically sound and whether the study satisfies 

any applicable data requirements. Reviewers are expected to review and to determine for each study, on a case-bycase basis, whether it is scientifically sound and provides sufficient information to satisfy applicable data 

requirements. Studies that fail to meet any of the conditions may be accepted, if appropriate; similarly, studies that 

meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of 

factors related to the test methodology and results in determining the acceptability of the study. 
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 2 of 20

EXECUTIVE SUMMARY:

The effect of Nicosulfuron, (DPX-V9360, 75WG), on the vegetative vigor of monocot crops (oat, Avena sativa; 

onion, Allium cepa; sorghum, Sorghum bicolor; wheat, Triticum aestivum) and dicot crops (cucumber, Cucumis 

sativa; oilseed rape, Brassica napus; pea, Pisum sativum, soybean, Glycine max; sugarbeet, Beta vulgaris; and 

tomato, Lycopersicon esculentum) was studied at nominal concentrations of 0 (negative), 0.00010, 0.00021, 

0.00042, 0.00083, 0.0017, 0.0033, 0.0067, 0.013, 0.027, and 0.053 lb as/A. Measured application rate for the 

highest concentration was reported and ranged from 0.047 to 0.055 lb as/A. 

The growth medium used in the vegetative vigor test was an artificial greenhouse soil mixture for oat, onion, 

sorghum, cucumber, oilseed rape, sugarbeet, and tomato (loamy sand, pH 7.0, organic matter 2.7%) and for pea, 

soybean, and wheat (loamy sand, pH 7.0, organic matter 2.7%). On day 21 the surviving plants per pot were 

recorded, and plant height and dry weight were measured. 

Negative control survival was 100% for all species. Based on the study author results, there was significant inhibition 

in survival of cucumber and oat at the highest treatment level, 0.055 lb as/A (p<0.05; Dunnett’s test). The reviewer 

found that the 35% inhibition in survival of cucumber at the highest treatment level was significant, but the 20% 

inhibition in survival of oat at the highest treatment level was not significant (p<0.05; Mann-Whitney U Two-Sample 

test). 

The reviewer found an inhibition in height for all species except soybean. There were significant inhibitions in height 

at maximum inhibitions of 53, 68, 65, 37, 56, 47, 20, and 42% for cucumber, oat, oilseed rape, onion, sorghum, 

sugarbeet, tomato and wheat, respectively compared to the negative control and responses were dose-dependent

(p<0.05; Williams test). The reviewer also found significant inhibition in height of up to 40% for pea at the highest 

treatment level, 0.55 lb as/A, however the response was not dose-dependent (p<0.05; Dunnett’s test). 

 

The reviewer also found significant inhibitions in dry weight in all species except soybean. There were significant 

inhibitions in dry weight with maximums of up to 75, 86, 93, 49, 58, 71, 69 and 87% for cucumber, oat, oilseed rape, 

onion, sorghum, sugarbeet, tomato and wheat, respectively, compared to the negative control and responses were 

dose-dependent (p<0.05; Williams test). The reviewer also found significant inhibition in dry weight of 32% for pea 

at the highest treatment level, 0.055 lb as/A, however, the effect was not dose-dependent (p<0.05; Dunnett’s test).

The occurrence of phytotoxic effects increased in severity and prevalence with increasing test concentrations; 

symptoms included discoloration, leaf deformation, compressed growth and necrosis. Mean effects were maximums 

of 18 to 100% over all the species tested; oilseed rape and cucumber had the most significant effects of 18 and 20%, 

respectively (100 indicates normal seedling, 0 indicates complete mortality). Effects were dose-related. 

The most sensitive monocot species was sorghum based on dry weight, with NOEC and IC25 values of 0.0033 and 

0.00613 lb as/A, respectively. The most sensitive dicot species was oilseed rape based on dry weight, with NOEC 

and IC25 values of 0.00083 and 0.00208 lb as/A, respectively.

 

Maximum Labeled Rate: 0.053 lb as/A (equivalent to 60 g as/ha).

Most sensitive monocot: Sorghum, based on dry weight

EC50/IC50: 0.0249 lb as/A 95% C.I.: 0.0196-0.0315 lb as/A

EC25/IC25: 0.00613 lb as/A 95% C.I.: 0.00417-0.00862 lb as/A

EC05/IC05: 0.000817 lb as/A 95% C.I.: 0.000133-0.00162 lb as/A

NOEC: 0.0033 lb as/A

Slope: N/A 95% C.I.: N/A
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 3 of 20

Most sensitive dicot: Oilseed Rape, based on dry weight

EC50/IC50: 0.00495 lb asA 95% C.I.: 0.00424-0.00579 lb as/A

EC25/IC25: 0.00208 lb as/A 95% C.I.: 0.00163-0.00258 lb as/A

EC05/IC05: 0.000595 lb as/A 95% C.I.: 0.000154-0.000925 lb as/A

NOEC: 0.00083 lb as/A

Slope: N/A 95% C.I.: N/A

Table 1 (Tier II studies). Summary of most sensitive parameters by species (lbs as/A).

Species Endpoint NOEC EC05/IC05 EC25/IC25 EC50/IC50

Cucumber Dry weight 0.0017 0.000369 0.00262 0.0102

Oat Dry weight 0.0067 0.00537 0.0114 0.0192

Oilseed Rape Dry weight 0.00083 0.000595 0.00208 0.00495

Onion Dry weight 0.0067 0.00645 0.0235 0.0577

Pea Height 0.027 0.0247 0.0439 0.0655

Sorghum Dry weight 0.0033 0.000817 0.00613 0.0249

Soybean None 0.055 >0.055 >0.055 >0.055

Sugarbeet Dry weight 0.0033 0.00186 0.00764 0.0204

Tomato Dry weight 0.0017 0.000532 0.00384 0.0152

Wheat Dry weight 0.0067 0.00433 0.0105 0.0194

This study is scientifically sound and is classified as acceptable.

I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The methods used in conducting this study were based on procedures 

specified in the U.S. EPA Series 850 – Ecological Effects Test Guidelines 

OPPTS Number 850.4250 (1996). Deviations from OPPTS 850.4250 were 

noted.

1. The physico-chemical properties of the test material were not reported.

The deficiency and deviations did not have an impact on the acceptability of this study.

COMPLIANCE: Signed and dated GLP, Quality Assurance and No Data Confidentiality 

statements were provided. This study was conducted in compliance with 

FIFRA Good Laboratory Practice Standards as published by the U.S. EPA, 

40 CFR Part 160 (1989) and the OECD Principles of Good Laboratory 

Practice (ENV/MC/CHEM (98)17). 

A. MATERIALS:

1. Test Material Nicosulfuron (DPX-V9360) 75WG plus Isodecylalcohol Ethoxylated 

(IN-KG691) Surfactant; formulation containing 75.1% as

Description: Solid powder

Lot No./Batch No.: APR02EL025/DPX-V9630-119
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 4 of 20

Purity: 75.1%

Stability of compound 

under test conditions: Analytical recovery of test substance from the spray solutions used in the 

four test application dates of November 5, November 20, December 16 and 

February 12 was 99.4-103.8%, 78.1-97.5%, 106.3-111.9% and 101.9-

103.1%, respectively, (n=8) at a nominal concentration of 0.053 lb as/A.

The certificate of analysis reported that 100% of the test material remains in 

the delivery vehicle after 1 hour under agitation.

(OECD recommends chemical stability in water and light)

Storage conditions of 

test chemicals: The test material was stored under ambient conditions.

Table 2. Physical/chemical properties of Nicosulfuron (DPX-V9360).

Parameter Values Comments

Water solubility at 20 ̊C Not reported

Vapor pressure Not reported

UV absorption Not reported

pKa Not reported

Kow Not reported

2. Test organism:

Monocotyledonous species: Oat (Avena sativa, Gramineae; Ogle), Onion (Allium cepa, Liliaceae; Yellow 

Granex Hybrid), Sorghum (Sorghum bicolor, Gramineae; Pioneer 8313-2103), and Wheat (Triticum 

aestivum, Gramineae; Sisson). EPA recommends four monocots in two families, including corn.

Dicotyledonous species: Cucumber (Cucumis sativa, Cucurbitaceae; Straight Eight), Oilseed Rape 

(Brassica napus, Brassicaceae; Wichita), Pea (Pisum sativum, Leguminosae; Little Marvel), Soybean 

(Glycine max, Leguminosae; Williams B2), Sugarbeet (Beta vulgaris, Chenopodiaceae; 1309R), and 

Tomato (Lycopersicon esculentum, Solanaceae; Beefsteak). EPA recommends six dicots in four families, 

including soybean and a root crop.

OECD recommends a minimum of three species selected for testing, at least one from each of the following categories: 

Category 1: ryegrass, rice, oat, wheat, and sorghum; Category 2: mustard, rape, radish, turnip, and Chinese cabbage; 

Category 3: vetch, mung bean, red clover, fenugreek, lettuce, and cress.

Seed source: Cucumber, Onion, Pea and Tomato obtained from Chesmore Seed Company; Oat obtained 

from Illinois Foundation Seed; Oilseed Rape obtained from Kansas State University Department of 

Agronony; Sorghum obtained from Syngenta Seeds; Soybean obtained from Missouri Foundation Seeds; 

Sugarbeet obtained from Betaseed; and Wheat obtained from Virginia Crop Improvement Association.

Prior seed treatment/sterilization: It was not stated if the seeds were treated with any type of fungicides, 
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 5 of 20

insecticides, or pesticides.

Historical % germination of seed: Cucumber, 85%; oat, 97%; oilseed rape, 95%; onion, 82%; pea, 81%; 

sorghum, 85%; soybean, 88%; sugarbeet, 96%; tomato, 80%; and wheat, 94%.

Seed storage, if any: Not reported.

B. STUDY DESIGN:

1. Experimental Conditions:

a. Limit test: None.

b. Range-finding study: None.

c. Definitive Study

Table 3: Experimental Parameters – Vegetative Vigor.

Parameters Vegetative Vigor

Details Remarks

Criteria

Duration of the test 21 days

Recommended test duration is 14-21 days.

OECD recommends that the test be terminated no 

sooner than 14 days after 50 percent of the control 

seedlings have emerged

Number of 

seeds/plants/species/ 

replicate

Five pots (replicates) with 4 seeds 

per pot.

Five plants per replicate are recommended.

Number of plants retained 

after thinning

Thinned to one seed per pot prior 

to application; Application at 1-4 

leaf stage. 

Number of replicates

Control:

Adjuvant control:

Treated:

5

N/A

5

Four replicates per dose should be used.

OECD recommends a minimum of four replicates 

per treatment
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 6 of 20

Parameters Vegetative Vigor

Details Remarks

Criteria

Test concentrations (lb 

as/A)

Nominal:

Measured: 

0 (negative), 0.00010, 0.00021, 

0.00042, 0.00083, 0.0017, 0.0033, 

0.0067, 0.013, 0.027, and 0.053lb 

as/A. 

Highest treatment concentration 

was measured and ranged from 

0.047 to 0.055 lb as/A. 

Five test concentrations should be used with a dose 

range of 2X or 3X progression

OECD recommends three concentrations, 

preferably with application rates equivalent to 0.0 

(control), 1.0, 10.0 and 100 mg substance per kg of 

oven-dried soil.

Method and interval of 

analytical verification

LOQ:

LOD:

Concentration of DPX-V9360-119 

in the negative control and highest 

treatment solution was determined

by HPLC using an Agilent G1300 

Series HPLC equipped with an 

Agilent G1314A UV detector and 

a Discovery Cl 8 column (150 mm 

x 4.6 mm I.D.).

0.000021 lb as/A

N/A

Adjuvant (type, percentage, 

if used) N/A

Test container (pot)

Size/Volume

Material: 

(glass/polystyrene)

Onion pot was 10 cm diameter 

and 7 cm deep; all other species, 

15 cm diameter and 10 cm deep.

Plastic

Non-porous containers should be used.

OECD recommends that non-porous plastic or 

glazed pot be used.

Growth facility Greenhouse

Method/depth of seeding Plants were thinned prior to 

application. Cucumber, oat, pea, 

sorghum, soybean and wheat 

seeds were planted 20 mm below 

the surface and oilseed rape, 

onion, sugarbeet and tomato seeds 

were planted 6 mm deep.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 7 of 20

Parameters Vegetative Vigor

Details Remarks

Criteria

Test material application

Application time including 

the plant growth stage

Number of applications

Application interval

Method of application

Application at the 1-4 true leaf 

growth stage.

1

N/A- single application

The test material was applied 

using a Devries Research Track 

Sprayer (4004 E Tee-Jet nozzle), 

40 psi; 72 cm above the soil 

surface.

Details of soil used

Geographic location

Depth of soil collection

Soil texture

% sand

% silt

% clay

pH:

% organic carbon

CEC

Moisture at 1/3 atm (%)

Geographic location

Depth of soil collection

Soil texture

% sand

% silt

% clay

pH:

% organic carbon

CEC

Moisture at 1/3 atm (%)

For oat, onion, sorghum, 

cucumber, oilseed rape, sugarbeet, 

and tomato:

N/A

N/A

Silt loam

22

56

22

7.0

N/A

20.8 meq/100g

30.1%

for pea, soybean, and wheat: 

N/A

N/A

Silt loam

15

64

21

7.0

N/A

17.3

29.1

Organic matter: 2.7%

Organic Matter: 2.5%

Soil mixes containing sandy loam, loam, or clay 

loam soil with no greater than 2% organic matter 

are preferable. Glass beads, rock wool, and 100% 

acid washed sand are not preferred.

OECD prefers the soil to be sieved (0.5 cm) to 

remove coarse fragments. Carbon content should 

not exceed 1.5% (3% organic matter). Fine particles 

(under 20um) makeup should be between 10 and 

20%. The recommended pH is between 5.0 and 7.5.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 8 of 20

Parameters Vegetative Vigor

Details Remarks

Criteria

Details of nutrient medium, 

if used N/A

Watering regime and 

schedules

Water source/type:

Volume applied:

Interval of application:

Method of application:

Well water demineralized by 

reverse osmosis.

Nominal spray volume was 375 

L/ha; calibrations of sprayer 

system indicated actual spraying 

rates of 369-380 L/ha.

One application per species.

The plants were bottom watered.

EPA prefers that bottom watering be utilized for 

seedling emergence studies so that the chemical is 

not leached out of the soil during the test.

Any pest control 

method/fertilization, if used

None reported

Test conditions

Temperature:

Photoperiod:

Light intensity and quality:

Relative humidity:

Temperature:

Photoperiod:

Light intensity and quality:

Relative humidity:

Temperature:

Photoperiod:

Light intensity and quality:

Relative humidity:

Temperature:

Cucumber and Tomato

21.7-26.7 ̊C (day)

12.6-21.7 ̊C (night)

16L:8D

Natural light supplemented with 

high intensity grow lights.

104.3-771.8 uEins/m2/s

43-72% (day)

Oilseed Rape and Sugarbeet

21.7-27.9 ̊C (day)

16.0-23.6 ̊C (night)

16L:8D

Natural light supplemented with 

high intensity grow lights.

121.9-753.0 uEins/m2/s

32-74% (day)

Oat and Sorghum

22.5-32.3 ̊C (day)

16.4-21.7 ̊C (night)

16L:8D

Natural light supplemented with 

high intensity grow lights.

77.34-509.4 uEins/m2/s

29-70% (day)

Onion

26.3-31.5 ̊C (day)

11.4-23.2 ̊C (night)
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 9 of 20

Parameters Vegetative Vigor

Details Remarks

Criteria

Photoperiod:

Light intensity and quality:

Relative humidity:

Temperature:

Photoperiod:

Light intensity and quality:

Relative humidity:

16L:8D

Natural light supplemented with 

high intensity grow lights.

208.0-519.7 uEins/m2/s

16-52% (day)

Pea, Soybean and Wheat

22.1-32.8 ̊C (day)

17.5-24.4 ̊C (night)

16L:8D

Natural light supplemented with 

high intensity grow lights.

151.6-563.3 uEins/m2/s

26-63% (day)

EPA prefers that the cold vs warm loving plants be 

tested in two separate groups to optimize plant 

growth.

OECD prefers that the temperature, humidity and 

light conditions be suitable for maintaining normal 

growth of each species for the test period.

Reference chemical (if 

used)

Name:

Concentrations: 

DPX-V9360 Lot 116

97.9% purity

Other parameters, if any None

2. Observations:

Table 4: Observation Parameters – Vegetative Vigor.

Parameters Vegetative Vigor

Details Remarks

Parameters measured (e.g., number 

of germinated seeds, emerged 

seedlings, plant height, dry weight 

or other endpoints)

- Survival

- Height

- Total dry weight

- Phytotoxicity

Measurement technique for each 

parameter 

Phytotoxicity was visually

determined. Survival was 

defined as the percent of 

emerged. Height was 

measured. Mean plant 

weight was estimated by 

measuring the total dry 

weight following oven 
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 10 of 20

drying (60 to 80oC for at 

least 48 hours). 

Observation intervals Each pot was inspected 

weekly and phytotoxicity 

assessments performed. 

Shoot height and dry 

weight were recorded at 

study termination.

Other observations, if any N/A

Were raw data included? Yes

Phytotoxicity rating system, if used 0- Complete mortality; 

100 – normal seedling.

Frans, Robert E. and Ronald E. Talbert. 1977. 

Design of Field Experiments and the 

Measurement and Analysis of Plant Responses, 

pp. 15-23, in B. Truelove, ed., Research 

Methods in Weed Science. Southern Weed 

Science Society, Auburn University, Alabama.

II. RESULTS and DISCUSSION:

A. INHIBITORY EFFECTS:

1. Vegetative Vigor: 

Negative control survival was 100% for all species. Based on the study author results, there was significant 

inhibition in survival of cucumber and oat at the highest treatment level, 0.055 lb as/A (p<0.05; Dunnett’s 

test). The reviewer found that the 35% inhibition in survival of cucumber at the highest treatment level was 

significant, but the 20% inhibition in survival of oat at the highest treatment level was not significant 

(p<0.05; Mann-Whitney U Two-Sample test). 

The study author and reviewer found an inhibition in height for all species except soybean. The reviewer 

found significant inhibitions in height at maximum inhibitions of 53, 68, 65, 37, 56, 47, 20, and 42% for 

cucumber, oat, oilseed rape, onion, sorghum, sugarbeet, tomato and wheat, respectively compared to the 

negative control and responses were dose-dependent (p<0.05; Williams test). The reviewer also found 

significant inhibition in height of up to 40% for pea at the highest treatment level, 0.55 lb as/A, however the 

response was not dose-dependent (p<0.05; Dunnett’s test). 

 

The study author and reviewer also found significant inhibitions in dry weight in all species except soybean. 

The reviewer found significant inhibitions in dry weight were maximums of up to 75, 86, 93, 49, 58, 71, 69 

and 87% for cucumber, oat, oilseed rape, onion, sorghum, sugarbeet, tomato and wheat, respectively, 

compared to the negative control and responses were dose-dependent (p<0.05; Williams test). The reviewer 

also found significant inhibition in dry weight of 32% for pea at the highest treatment level, 0.055 lb as/A, 

however, the effect was not dose-dependent (p<0.05; Dunnett’s test). 

The occurrence of phytotoxic effects increased in severity and prevalence with increasing test 

concentrations; symptoms included discoloration, leaf deformation, compressed growth and necrosis. Mean 

effects were maximums of 18 to 100% over all the species tested; oilseed rape and cucumber had the most 
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 11 of 20

significant effects of 18 and 20%, respectively (100 indicates normal seedling, 0 indicates complete 

mortality). Effects were dose-related. 

Based on the study authors results, the most sensitive monocot species was wheat based on dry weight, with 

NOEC and IR50 values of 0.0067 and 0.019 lb as/A, respectively. The most sensitive dicot species was 

oilseed rape based on dry weight, with NOEC and IR50 values of 0.00083 and 0.0055 lb as/A, respectively.

B. REPORTED STATISTICS: 

Survival, dry weight, and height means and standard deviations were determined for control and treatment groups.

NOEC values were determined using Dunnett’s trend test. All statistical determinations were made with 95% 

certainty. Estimates of the ERx values and their confidence limits for dry weight and growth height were determined 

by non-linear regression analysis using a four-parameter logistic (sigmoid-shaped) model with two parameters fixed 

(100 and 0% inhibition). A probit analysis developed by Finney (1971) was used in the non-linear regression 

analysis of survival data. These analyses were conducted using SAS, version 6.12 (1996). Nominal concentrations 

were used for all analyses. Study authors calculated survival based on number planted.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

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Table 5: Effect of Nicosulfuron (DPX-V9360) on 21-Day Vegetative Vigor

Species Results summary for biomass (lbs as/A)*

weight (g) NOEC IC5 95%CI IC25 95%CI IC50 95%CI slope 95%CI

Cucumber 1.83-7.68 0.0017 ND N/A 0.0032 0.0021-

0.0042

0.0096 0.0073-

0.0119

N/A N/A

Oat 0.229-1.82 0.0067 ND N/A 0.0125 0.0099-

0.0152

0.0194 0.0165-

0.0224

N/A N/A

Oilseed Rape 0.458-6.89 0.00083 ND N/A 0.0030 0.0026-

0.0035

0.0055 0.0049-

0.0061

N/A N/A

Onion 0.116-0.229 0.013 ND N/A 0.0227 0.0182-

0.0273

0.0525 0.0413-

0.0638

N/A N/A

Pea 2.77-4.17 0.027 ND N/A 0.0477 0.0360-

0.0594

>0.053 N/A N/A N/A

Sorghum 0.175-0.42 0.0033 ND N/A 0.0052 0.0032-

0.0072

0.0234 0.0157-

0.0311

N/A N/A

Soybean 8.0-8.86 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Sugarbeet 1.34-4.86 0.0033 ND N/A 0.0079 0.0059-

0.0099

0.0190 0.0154-

0.0226

N/A N/A

Tomato 1.52-5.57 0.0033 ND N/A 0.0060 0.0034-

0.0086

0.0180 0.0122-

0.0238

N/A N/A

Wheat 0.36-2.71 0.0067 ND N/A 0.0104 0.0078-

0.0130

0.0186 0.0154-

0.0220

N/A N/A

ND- Not determined. NC- Not calculable.

* NOEC determined using Dunnett’s trend test (p<0.05); IC25 and IC50 and their 95% confidence intervals estimated using non-linear regression 

analysis.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 13 of 20

Table 5a: Effect of Nicosulfuron (DPX-V9360) on 21-Day Vegetative Vigor

Species Results summary for height (lbs as/A)*

height 

(mm)

NOEC IC5 95%CI IC25 95%CI IC50 95%CI slope 95%CI

Cucumber 62-156 0.0067 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Oat 167-534 0.0067 ND N/A 0.0166 0.0141-

0.0191

0.0299 0.0266-

0.0331

N/A N/A

Oilseed Rape 70.6-224 0.0067 ND N/A 0.0088 0.0064-

0.0113

0.0196 0.0157-

0.0235

N/A N/A

Onion 115-184 0.013 ND N/A 0.0340 0.0273-

0.0407

>0.053 N/A N/A N/A

Pea 182-301 0.027 ND N/A 0.0416 0.0354-

0.0478

>0.053 N/A N/A N/A

Sorghum 109-247 0.0033 ND N/A 0.0068 0.0050-

0.0085

0.292 0.0221-

0.0362

N/A N/A

Soybean 282-317 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Sugarbeet 106-200 0.0033 ND N/A 0.0136 0.0112-

0.0159

>0.053 N/A N/A N/A

Tomato 96-130 0.013 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Wheat 225-389 0.0067 ND N/A 0.0163 0.0130-

0.0195

>0.053 N/A N/A N/A

ND- Not determined. NC- Not calculable.

* NOEC determined using Dunnett’s trend test (p<0.05); IC25 and IC50 and their 95% confidence intervals estimated using non-linear regression 

analysis.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 14 of 20

Table 5b: Effect of Nicosulfuron (DPX-V9360) on 21-Day Vegetative Vigor

Species Results summary for survival (lbs as/A), based on # planted*

% NOEC EC5 95%CI EC25 95%CI EC50 95%CI slope 95%CI

Cucumber 65-100 0.027 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Oat 80-100 0.027 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Oilseed 

Rape

95-100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Onion 100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Pea 100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Sorghum 100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Soybean 100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Sugarbeet 90-100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Tomato 100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

Wheat 100 0.053 ND N/A >0.053 N/A >0.053 N/A N/A N/A

ND- Not determined. NC- Not calculable.

* NOEC determined using Dunnett’s trend test (p<0.05); EC25 and EC50 and their 95% confidence intervals estimated using non-linear regression 

analysis.

Plant Injury Index*

Control Cucumber Oat Oilseed

Rape

Onion Pea Sorghum Soybean Sugarbeet Tomato Wheat Formulation 

Blank

90-100 20-92 30-100 18-100 80-100 78-100 50-100 96-100 24-100 50-100 32-100 N/A

*0- complete mortality, 100 –no effect.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 15 of 20

C. VERIFICATION OF STATISTICAL RESULTS BY THE REVIEWER:

All analyses were conducted comparing treated to the negative control. These analyses were conducted using 

CETIS version 1.8.7.12 and backend settings approved for use by EFED on 3/25/14. Data for each endpoint 

were tested to determine if their distributions were normal and if their variances were homogeneous using 

Shapiro-Wilk’s and Levene’s tests, respectively. Data that satisfied these assumptions were subjected to 

Dunnett’s and William’s tests, and data that did not satisfy these assumptions were subjected to the nonparametric MannWhitney-U and Jonckheere’s tests. Nominal concentrations were used for all analyses except 

the highest treatment level. Linear (survival and emergence) and nonlinear (height and dry weight) regression 

models were used to interpret EC/ICx values.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 16 of 20

Table 6: Effect of Nicosulfuron (DPX-V9360) on 21-Day Vegetative Vigor

Species Results summary for biomass (lbs as/A)

weight (g) NOEC IC5 95%CI IC25 95%CI IC50 95%CI slope 95%CI

Cucumber11.83-7.68 0.0017 0.000369 1.82x10-5-

0.00076

0.00262 0.0018-

0.00364

0.0102 0.00824-

0.0126

N/A N/A

Oat20.229-1.82 0.0067 0.00537 0.00175-

0.00709

0.0114 0.00921-

0.0136

0.0192 0.017-

0.0216

N/A N/A

Oilseed 

Rape3

0.458-6.89 0.00083 0.000595 0.000154-

0.000925

0.00208 0.00163-

0.00258

0.00495 0.00424-

0.00579

N/A N/A

Onion40.116-0.229 0.0067 0.00645 0.00236-

0.00982

0.0235 0.0189-

0.0286

0.0577 0.0477-

0.0699

N/A N/A

Pea52.77-4.17 0.027 0.0287 N/A-0.045 0.0501 0.0397-

0.0601

0.0738 0.0433-

0.126

N/A N/A

Sorghum60.175-0.42 0.0033 0.000817 0.000133-

0.00162

0.00613 0.00417-

0.00862

0.0249 0.0196-

0.0315

N/A N/A

Soybean 8.0-8.86 0.055 >0.055 N/A >0.055 N/A >0.055 N/A N/A N/A

Sugarbeet71.34-4.86 0.0033 0.00186 0.000617-

0.00295

0.00764 0.00574-

0.00983

0.0204 0.0174-

0.0239

N/A N/A

Tomato81.52-5.57 0.0017 0.000532 N/A0.001170.00384 0.00245-

0.00567

0.0152 0.0118-

0.0195

N/A N/A

Wheat90.36-2.71 0.0067 0.00433 N/A0.006080.0105 0.00816-

0.013

0.0194 0.0168-

0.0225

N/A N/A

ND- Not determined. NC- Not calculable.

1 Significant decrease in cucumber weight, inhibition of 29 to 75% beginning at the 0.0033 lb as/A treatment compared to the negative control 

(Williams test, p<0.05).

2 Significant decrease in oat weight, inhibitions of 33, 69 and 86% at the 0.013, 0.027 and 0.047 lbs as/A treatments compared to the negative 

control (Williams test, p<0.05).

3 Significant decrease in oilseed rape weight, inhibition of 27 to 93% beginning at 0.0033 lb as/A treatment compared to the negative control 

(Williams test, p<0.05).

4 Significant decrease in onion weight, inhibition of 12, 32 and 49% at the 0.013, 0.027 and 0.057 lb as/A treatments compared to the negative 

control (Williams test, p<0.05).

5 Significant decrease in pea weight, inhibition of 32% at 0.055 lbs as/A treatment compared to the negative control (Dunnett’s test, p<0.05).

6 Significant decrease in sorghum weight, inhibitions of 24 to 58% beginning at the 0.0067 lbs as/A treatment compared to the negative control 

(Williams test, p<0.05).

7 Significant decrease in sugarbeet weight, inhibition of 18 to 71% beginning at the 0.0067 lb as/A treatment compared to the negative control

(Williams test, p<0.05).

8 Significant decrease in tomato weight, inhibition of 19 to 69% beginning at the 0.0033 lb as/A treatment compared to the negative control 

(Williams test, p<0.05).

9 Significant decrease in wheat weight, inhibition of 37, 67 and 87% at the 0.013, 0.027 and 0.055 lb as/A treatments compared to the negative 

control (Williams test, p<0.05).
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 17 of 20

Table 6a: Effect of Nicosulfuron (DPX-V9360) on 21-Day Vegetative Vigor

Species Results summary for height (lbs as/A)

height 

(mm)

NOEC IC5 95%CI IC25 95%CI IC50 95%CI slope 95%CI

Cucumber162-156 0.0067 >0.0067 N/A >0.0067 N/A >0.0067 N/A N/A N/A

Oat2167-534 0.0067 0.00647 0.00426-

0.00815

0.0156 0.0134-

0.0178

0.0286 0.0265-

0.0309

N/A N/A

Oilseed Rape370.6-224 0.0067 0.00144 0.000384-

0.0024

0.00671 0.0049-

0.00886

0.0196 0.0165-

0.0234

N/A N/A

Onion4115-184 0.013 0.00776 0.00301-

0.012

0.032 0.0261-

0.0386

0.0858 0.0619-

0.119

N/A N/A

Pea5182-301 0.027 0.0247 N/A0.03250.0439 0.0378-

0.0498

0.0655 0.056-

0.0766

N/A N/A

Sorghum6109-247 0.00021 0.00123 0.00053-

0.002

0.00819 0.00636-

0.0103

0.0306 0.0257-

0.0364

N/A N/A

Soybean 282-317 0.055 >0.055 N/A >0.055 N/A >0.055 N/A N/A N/A

Sugarbeet7106-200 0.0033 0.0025 0.00146-

0.00362

0.0163 0.0137-

0.0191

0.0599 0.0496-

0.0723

N/A N/A

Tomato896-130 0.0033 0.00236 0.000642-

0.00524

0.0738 0.0356-

0.14

0.808 0.123-5.31 N/A N/A

Wheat9225-389 0.0067 0.00205 0.000984-

0.00324

0.0159 0.0129-

0.0195

0.0663 0.051-

0.0862

N/A N/A

ND- Not determined. NC- Not calculable.

1 Significant decrease in cucumber height, inhibition of 49, 52 and 53% at 0.013, 0.027 and 0.055 lb as/A treatment compared to the negative 

control (Williams test, p<0.05).

2 Significant decrease in oat height, inhibitions of 14, 54 and 68% at the 0.013, 0.027 and 0.047 lbs as/A treatments compared to the negative 

control (Williams test, p<0.05).

3 Significant decrease in oilseed rape height, inhibition of 54, 62 and 65% at 0.013, 0.027 and 0.055 lb as/A treatment compared to the negative 

control (Jonckheere-Terpstra Step-Down test, p<0.05). 

4 Significant decrease in onion height, inhibition of 23 and 37% at the 0.027 and 0.057 lb as/A treatments compared to the negative control 

(Williams test, p<0.05).

5 Significant decrease in pea height, inhibition of 40% at 0.055 lbs as/A treatment compared to the negative control (Dunnett’s test, p<0.05).

6 Significant decrease in sorghum height, inhibitions of 11 to 56% beginning at the 0.00042 lbs as/A treatment compared to the negative control 

(Williams test, p<0.05).

7 Significant decrease in sugarbeet height, inhibition of 13 to 47% beginning at the 0.0067 lb as/A treatment compared to the negative control 

(Williams test, p<0.05).

8 Significant decrease in tomato height, inhibition of 12 to 20% beginning at the 0.0067 lb as/A treatment compared to the negative control 

(Williams test, p<0.05).

9 Significant decrease in wheat height, inhibition of 25, 42 and 42% at the 0.013, 0.027 and 0.055 lb as/A treatments compared to the negative 

control (Williams test, p<0.05).
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 18 of 20

Table 6b: Effect of Nicosulfuron (DPX-V9360) on 21-Day Vegetative Vigor

Species Results summary for survival (lbs as/A), based on # planted

% NOEC EC5 95%CI EC25 95%CI EC50 95%CI slope 95%CI

Cucumber165-100 0.027 >0.027 N/A >0.027 N/A >0.027 N/A N/A N/A

Oat 80-100 0.047 NC N/A >0.047 N/A >0.047 N/A N/A N/A

Oilseed 

Rape

95-100 0.055 >0.055 N/A >0.055 N/A >0.055 N/A N/A N/A

Onion 100 0.057 >0.057 N/A >0.057 N/A >0.057 N/A N/A N/A

Pea 100 0.055 >0.055 N/A >0.055 N/A >0.055 N/A N/A N/A

Sorghum 100 0.047 >0.047 N/A >0.047 N/A >0.047 N/A N/A N/A

Soybean 100 0.055 >0.055 N/A >0.055 N/A >0.055 N/A N/A N/A

Sugarbeet 90-100 0.055 NC N/A >0.055 N/A >0.055 N/A N/A N/A

Tomato 100 0.055 >0.055 N/A >0.055 N/A >0.055 N/A N/A N/A

Wheat 100 0.055 >0.055 N/A >0.055 N/A >0.055 N/A N/A N/A

ND- Not determined. NC- Not calculable.

1 Significant decrease in cucumber survival, inhibition of 35% at 0.055 lb as/A treatment compared to the negative control (Mann-Whitney U TwoSample test, p<0.05).

Plant Injury Index*

Control Cucumber Oat Oilseed

Rape

Onion Pea Sorghum Soybean Sugarbeet Tomato Wheat Formulation 

Blank

90-100 20-92 30-100 18-100 80-100 78-100 50-100 96-100 24-100 50-100 32-100 N/A

* 0- complete mortality; 100 = normal seedling.

Most sensitive monocot: Sorghum, based on dry weight

EC50/IC50: 0.0249 lb as/A 95% C.I.: 0.0196-0.0315 lb as/A

EC25/IC25: 0.00613 lb as/A 95% C.I.: 0.00417-0.00862 lb as/A

EC05/IC05: 0.000817 lb as/A 95% C.I.: 0.000133-0.00162 lb as/A

NOEC: 0.0033 lb as/A

Slope: N/A 95% C.I.: N/A
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 19 of 20

Most sensitive dicot: Oilseed Rape, based on dry weight 

EC50/IC50: 0.00495 lb asA 95% C.I.: 0.00424-0.00579 lb as/A

EC25/IC25: 0.00208 lb as/A 95% C.I.: 0.00163-0.00258 lb as/A

EC05/IC05: 0.000595 lb as/A 95% C.I.: 0.000154-0.000925 lb as/A

NOEC: 0.00083 lb as/A

Slope: N/A 95% C.I.: N/A

D. STUDY DEFICIENCIES: 

1. The physico-chemical properties of the test material were not reported.

E. REVIEWER’S COMMENTS: 

The reviewer and study author results are in general agreement. Based on the study authors results, the 

most sensitive monocot species was wheat based on dry weight, with NOEC and IR50 values of 0.0067 and 

0.019 lb as/A, respectively. The most sensitive dicot species was oilseed rape based on dry weight, with 

NOEC and IR50 values of 0.00083 and 0.0055 lb as/A, respectively. The reviewer found the most sensitive 

monocot species was sorghum based on dry weight, which is different from the study author’s findings 

because the reviewers based the most sensitive species on the IC25 and the study author determined the most 

sensitive species from the IR50. The NOEC and IC25 values for sorghum, based on dry weight, were 0.0033 

and 0.00613 lb as/A, respectively. The reviewer agreed with the study author, finding the most sensitive 

dicot species was oilseed rape based on dry weight, with NOEC and IC25 values of 0.00083 and 0.00208 lb 

as/A, respectively. The reviewer’s results are presented in the Executive Summary and Conclusions 

sections of this DER.

The in-life portion of this study was initiated on September 30, 2003 and completed March 8, 2004.

F. CONCLUSIONS: 

This study is scientifically sound and is classified as acceptable. The most sensitive monocot species was 

sorghum based on dry weight, with NOEC and IC25 values of 0.0033 and 0.00613 lb as/A, respectively. The 

most sensitive dicot species was oilseed rape based on dry weight, with NOEC and IC25 values of 0.00083

and 0.00208 lb as/A, respectively.

Most sensitive monocot and IC25: Sorghum (dry weight, 0.00613 lb as/A).

Most sensitive dicot and IC25: Oilseed rape (dry weight, 0.00208 lb as/A).

III. REFERENCES: 

1. Finney, D. J. 1971. Probit Analysis, 3rd Ed. Cambridge University Press.

Cambridge, U.K.

2. Frans, R.E. and Talbert, R.E., Design of Field Experiments and the Measurement and Analysis of 

Plant Responses, pp. 15-23, in B. Truelove, ed., Research Methods in Weed Science. Southern Weed 

Science Society, Auburn University, Alabama, 1977.
Data Evaluation Record on the Acute Toxicity of Nicosulfuron (DPX-V9360) 75WG to 

Terrestrial Vascular Plants: Vegetative Vigor

PMRA Submission Number {..............} EPA MRID Number 49169705

Page 20 of 20

3. OECD, OECD Guideline for the Testing of Chemicals, Guideline 227, Terrestrial Plant Test: 

Vegetative Vigor Test. OECD, July 2006.

4. SAS Institute, Inc. SAS System for Windows, Release 6.12. Cary, NC, SAS Institute, Inc. 1996.