# Patent

## Title
Compounds from Antrodia camphorata having anti-inflammatory and anti-tumor activity

## Abstract

The present invention relates to novel mixture and maleic and succinic acid derivatives from mycelium of Antrodia Camphorata and the medical use thereof. The present invention relates to the composition or mycelium comprising the compounds of the invention.

## Background
FIELD OF THE INVENTION
The present invention relates to novel mixture and compounds from mycelium of Antrodia Camphorata and the use thereof. The present invention relates to the composition or mycelium comprising the compounds of the invention.
BACKGROUND OF THE INVENTION
The fruiting body of Antrodia camphorata (Polyporaceae, Aphyllophorales) is well known in Taiwan as a traditional Chinese medicine. It grows only on the inner heartwood wall of the endemic evergreen Cinnamomun kanehirai (Hay) (Lauraceae) in Taiwan. It is rare and has not been cultivated. The fruiting bodies have been used for treating of food and drug intoxication, diarrhea, abdominal pain, hypertension, itchy skin, and liver cancer. Very few biological activity studies have been reported hitherto.
Antrodia camphorata also known as “niu-chang-chih” or “niu-chang-ku” in Taiwan, was recently reported as a new fungus species characterized by the cylindrical shape of its basidiospores appearing in fruiting bodies, weakly amyloid skeletal hyphae, bitter taste and light cinnamon resupinate to pileate basidiocarps, as well as chlamydospores and anthroconidia in pure culture. The growth of this new fungus species is extremely slow and restricted to an endemic tree species, Cinnamomum kanehirai Hay (Lauraceae), as the only host. The detailed characterization and taxonomic position of Antrodia camphorata were described in Wu, S.-H., et al., Antrodia cinnamomea (“niu-chang-chih”), New combination of a medicinal fungus in Taiwan, Bot. Bull. Acad. Sin. 38: 273–275 (1997).
In Taiwanese folk medicine, the fruiting bodies of Antrodia camphorata are believed to have certain medical effects. According to the traditional way, the fruiting bodies are ground into dry powder or stewed with other herbal drugs for oral uptake to treat conditions caused by poisoning, diarrhea, abdominal pain, hypertension, skin itches and liver cancer. However, few pharmacological or clinical study in these aspects has appeared in literature to date. Because of the stringent host specificity and rarity in nature, as well as the failure of artificial cultivation, “niu-chang-chih” is very expensive in Taiwan. In recent years, the fruiting bodies of this fungus with high quality have been sold at an extremely high price of around U.S.$ 15,000 per kg.
SUMMARY OF THE INVENTION
Accordingly, an object of the present invention is to provide novel mixture from mycelium of Antrodia Camphorata. 
Another object of the present invention is to provide novel compounds from mycelium of Antrodia Camphorata. 
Further object of the present invention is to provide novel composition comprising the compounds of the invention.
Further object of the present invention is to provide novel mycelium of Antrodia Camphorata comprising the compounds of the invention.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows HMBC correlations of compound 2.
FIG. 2 shows the compounds of the invention.
FIG. 3 shows NOE (nuclear Overhauser effect) correlations of compounds 4 and 5 of the invention.
FIG. 4(a)-(d) shows test results of compound 3 of the invention.
FIG. 5(a)-(c) shows test results of ACM, (Antrodia camphorata mycelia powder) H2O Extract.
FIG. 6(a)-(f) shows test results of ACM EtOH (ethyl alcohol) Extract.
FIG. 7(a)-(e) shows test results of compound 1 of the invention.



DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a compound having the formula




wherein

    X is N or O;
    R1 is C1-10 alkyloxy, C2-10 alkenyloxy, or C2-10 alkynyloxy;
    R2 is H, C1-10 alkyl, C2-10 alkenyl or C2-10 alkynyl; and
    R3 is absent, H or hydroxy;
    provided that if X is O, R3 is absent.


In the compound of the invention, the preferred R1 is C2-6 alkenyloxy, or C2-6 alkynyloxy; the more preferred R1 is C2-6 alkenyloxy substituted with C1-6 alkyl and the most preferred R1 is butenyloxy substituted with methyl. In the compound of the invention, the preferred R2 is C1-6 alkyl, the most preferred R2 is isobutyl.
Accordingly, the preferred compound of the invention is 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]furan-2,5-dione, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione, 3R*,4S*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, or 3R*,4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione.
The further preferred compound of the invention is 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione or 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione. The further preferred compound of the invention is 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione.
The present invention also provides a mixture from mycelium of Antrodia Camphorata, which comprises the compound of the invention. The mixture of the invention is prepared from water or organic solvent extract of mycelium of Antrodia Camphorata. The organic solvent includes but is not limited to alcohol (such as CH3OH, C2H5OH, C3H7OH), ester (such as acetyl acetate), alkane (such as hexane) and halogenated alkane (such as CH3Cl, C2H2Cl2). The preferred organic solvent is ethanol or alcoholic solvent without causing any side effect of human. The mixture of the invention can decrease systolic blood pressure or increase high density lipoprotein. In addition, the same mixture has central cholinergic agonism, hepatoprotection, anti-inflammation or anti-tumor activity. Especially, the mixture of the invention can inhibit tumor from the cells or tissues selected from the group consisting of liver, intestine, bone, blood, lymph and breast. The subject accepting the mixture of the invention includes but is not limited to human, mammal, mouse, rat, horse, pig, chicken, duck, dog and cat.
The present invention also provides a composition, which comprises the compound of the invention. The composition of the invention can decrease systolic blood pressure or increase high density lipoprotein. In addition, the composition of the invention has central cholinergic agonism, hepatoprotection, anti-inflammation or anti-tumor activity. Especially, the composition of the invention can inhibit tumor from the cells or tissues selected from the group consisting of liver, intestine, bone, blood, lymph and breast. The subject accepting the composition of the invention includes but is not limited to human, mammal, mouse, rat, horse, pig, chicken, duck, dog and cat.
The present invention also provides novel mycelium of Antrodia comprising the compounds of the invention. The preferred mycelium has at least 1% of the weight of raw mycelium being the total weight of the compounds 1–5 of the invention. The most preferred mycelium has at least 3% of the weight of raw mycelium being the total weight of the compounds 1–5 of the invention. The mycelium of Antrodia Camphorata is previously prepared according to submerged liquid fermentation such as T. L. M. Stamford et al., Food Science “Protein enrichment of cashew wastes for animal feeds” from http://www.unu.edu/unupress/food/8F101e/8F101E0b.htm.
EXAMPLES
The examples below are non-limiting and are merely representative of various aspects and features of the present invention.
General Experimental Procedures.
Melting points were measured on a Yanagimoto micro hot-stage melting point apparatus and uncorrected. Optical rotations were measured with a Jasco DIP-360 automatic polarimeter. UV spectra were measured with a Shimadzu UV-2200 recording spectrophotometer. IR spectra were measured with a Jasco FT/IR-230 infrared spectrometer. 1H- and 13C-NMR spectrum were measured with a Varian Unity Plus 500 spectrometer. EIMS and HR-EIMS were measured with a Jeol JMS-AX 505 HAD mass spectrometer at an ionization voltage of 70 eV. Column chromatography was carried out on silica gel BW-820 MH (normal phase) and Chromatorex-ODS DM1020T (reversed phase) (Fuji Silysia).
Extraction and Isolation
Antrodia camphorata mycelia powder (ACM) (60 g), from Simpson Biotech Co. Ltd., Taiwan, October 2001, were three times extracted with CHCl3 for 3 h under reflux. The CHCl3 extract (5.3 g) was chromatographed on silica gel eluted with n-hexane-acetone (19:1–14:6), and CHCl3-MeOH (1:1) to give nine fractions (Fr. 1–9). Fraction 2 was chromatographed on silica gel to give compound 1 (8.7 mg). Fraction 4 was chromatographed on normal and reversed phase silica gel to give compound 2 (13.6 mg). Fraction 5 was chromatographed on silica gel eluted with n-hexane-acetone (8:2) to give ergosterol peroxide (35.8 mg). Fraction 6 gave compound 3 (14.6 mg) by combination of normal and reversed phase silica gel column chromatography. Fraction 7 yielded a mixture of compounds 4 and 5 (4:1) by column chromatography. The mixture of compounds 4 and 5 were subsequently separated by preparative HPLC [column: Tosoh TSK-gel ODS-80TM (21.5×300 mm), mobile phase: CH3OH—H2O containing 0.1% TFA (70:30)].
3-Isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]furan-2,5-dione (compound 1):
yellow oil; UV (MeOH) λmax (log ε) 227 (4.1), 258 (3.9), 275 (3.8), 355 (3.4) nm; IR (CHCl3) νmax 1763 cm−1; 1H-NMR Table 1; 13C-NMR Table 2; EIMS m/z 314 [M]+ (100), 246 (100), 131 (100); HR-EIMS m/z 314.1523 (Calcd for C19H22O4, 314.1518).
3-Isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrole-2,5-dione (2):
yellow needles (n-hexane-AcOEt); mp 110–111° C.; UV (MeOH) λmax (log ε) 230 (4.3), 272 (3.5), 355 (3.7) nm; IR (CHCl3) νmax 1724 cm−1; 1H-NMR Table 1; 13C-NMR Table 2; EIMS m/z 313 [M]+ (8), 245 (100), 203 (77), 131 (28); HR-EIMS m/z 313.1681 (Calcd for C19H23NO3, 313.1678).
X-ray Crystallography of Compound 2:
Yellow needles were obtained by crystallization from n-hexane-AcOEt and selected for data collection. Crystal data: C19H23NO3; Mr=313.40; dimensions 0.15×0.02×0.02 mm; triclinic, space group P1 (#2), a=6.3505(5) Å, b=12.205(1) Å, c=12.560(2) Å, α=64.623(7)°, β=75.358(4)°, γ=84.681(5)°, V=850.9(2) Å3, Z=2, Dcalc=1.223 g/cm3, μ(MoKα)=0.82 cm−1, F000=336.00. Measurement was made on a Rigaku RAXIS-RAPID Imaging Plate diffractometer with graphite monochromated Mo-Kα (λ=0.71069 Å) radiation at 93 K. Of the 8950 reflections which were collected, 4745 were unique (Rint=0.108); equivalent reflections were merged. The crystal structure was solved by direct methods (SHELXS86) and refined by full-matrix least-squares. The non-hydrogen atoms were refined anisotropically. Hydrogen atoms were included but not refined. The final indices were R=0.074, Rw=0.099, with GOF (Guest Observer Facility)=1.06. The maximum and minimum peaks on the final difference Fourier map corresponded to 0.83 and −0.89 e−/Å3, respectively.
3-Isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione (Compound 3): yellow oil; UV (MeOH) λmax (log ε): 232.5 (4.3), 296 (3.7), 374 (3.7) nm; IR (CHCl3) νmax 1717 cm−1; 1H-NMR Table 1; 13C-NMR Table 2; EIMS m/z 329 [M]+ (12), 261 (100), 131 (50); HR-EIMS m/z: 329.1637 (Calcd for C19H23NO4, 329.1627).
3R*,4S*-1-Hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione (4): colorless oil; [α]D23+2.5° (c 0.2, MeOH); UV (MeOH) λmax (log ε): 225 (4.3), 275 (3.3), 283 (3.2) nm; IR (CHCl3) νmax 1715 cm−1; 1H-NMR Table 1; 13C-NMR Table 2; EIMS m/z 331 [M]+(2), 263 (67), 207 (66), 191 (30), 179 (40), 133 (64), 69 (100); HR-EIMS m/z 331.1747 (Calcd for C19H25NO4, 331.1783).
3R*,4R*-1-Hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione (5): colorless oil; [α]D23+3.0° (c 0.2, MeOH); UV (MeOH) λmax (log ε): 227 (4.3), 275 (3.4), 283 (3.3) nm; IR (CHCl3) νmax 1715 cm−1; 1H-NMR Table 1; 13C-NMR Table 2; EIMS m/z 331 [M]+ (1), 263 (45), 207 (50), 191 (75), 179 (30), 133 (100), 69 (92); HR-EIMS m/z 331.1766 (Calcd for C19H25NO4, 331.1783).
Ergosterol peroxide: colorless needles (n-hexane-acetone); mp 165–169° C. (lit2 mp 171–174° C.).
Cytotoxic Assays. The in vitro LLC tumor cell assay was carried out by sulforhodamin B (SRB) method. The 50% growth inhibition (ED50) was calculated by Probit method.
Results and Discussion
The CHCl3 extract of the mycelium of Antrodia Camphorata was repeatedly hromatographed on normal and reversed phase silica gel to afford five new maleic and succinic acid derivatives (compounds 1–5) together with ergosterol peroxide.
Table 1







TABLE 1







1H-NMR Spectral Data of Compounds 1–5 (δ ppm,


J=Hz) (500 MHz, CDCl3)












H
1
2
3
4
5





3
—
—
—
2.87(1H, m)
3.08(1H, m)


4
—
—
—
3.52(1H, d,
4.07(1H, d,






J=4.0)
J=8.0)


1′
2.59(2H, d,
2.51(2H, d,
2.50(2H, d,
1.51(1H, m)
1.02(1H, m)



J=7.0)
J=7.0)
J=7.0)
1.72~1,84(1H)
1.42~1.48(1H)


2′
2.12(1H, sep,
2.06(1H,
2.05(1H,
1.72~1.84
1.42~1.48



J=7.0)
sep, J=7.0)
sep, J=7.0)
(1H)
(1H)


3′
0.94(6H, d,
0.90(6H, d,
0.88(6H, d,
0.70(3H, d,
0.66(3H, d,



J=7.0)
J=7.0)
J=7.0)
J=6.5)
J=6.5)


4′



0.89(3H, d,
0.80(3H, d,






J=6.5)
J=6.5)


2′′, 6′′
7.50(2H, d,
7.50(2H, d,
7.50(2H, d,
7.07(2H, d,
6.96(2H, d,



J=9.0)
J=9.0)
J=9.0)
J=8.5)
J=9.0)


3′′, 5′′
7.02(2H, d,
6.95(2H, d,
6.98(2H, d,
6.87(2H, d,
6.84(2H, d,



J=9.0)
J=9.0)
J=9.0)
J=8.5)
J=9.0)


1′′′
4.57(2H, d,
4.56(2H, d,
4.55(2H, d,
4.47(2H, d,
4.47(2H, d,



J=6.6)
J=6.5)
J=6.9)
J=6.5)
J=6.5)


2′′′
5.50(1H, brt,
5.50(1H, brt,
5.49(1H, brt,
5.47(1H, brt,
5.47(1H, brt,



J=6.6)
J=6.5)
J=6.9)
J=6.5)
J=6.5)


4′′′
1.81(3H, s)
1.81(3H, s)
1.81(3H, s)
1.79(3H, s)
1.79(3H, s)


5′′′
1.76(3H, s)
1.76(3H, s)
1.76(3H, s)
1.73(3H, s)
1.73(3H, s)
















TABLE 2







13C-NMR Spectral Data for Compound 1–5 (δ ppm) (125


MHz, CDCl3)












C
1
2
3
4
5




















2
166.4
(s)
171.7
(s)
168.8
(s)
174.8
(s)
175.1
(s)


3
139.8
(s)
138.8
(s)a)
135.9
(s)a)
44.6
(d)
40.3
(d)


4
140.2
(s)
139.2
(s)a)
136.0
(s)a)
49.8
(d)
47.5
(d)


5
165.4
(s)
171.1
(s)
168.1
(s)
173.2
(s)
173.6
(s)


1′
33.6
(t)
32.8
(t)
33.2
(t)
40.4
(t)
35.3
(t)


2′
27.9
(d)
28.1
(d)
28.4
(d)
25.3
(d)
25.2
(d)


3′






21.3
(q)
21.8
(q)



22.7
(q)
22.7
(q)
23.0
(q)


4′






23.0
(q)
22.4
(q)


1′′
119.9
(s)
121.2
(s)
120.8
(s)
127.9
(s)
125.1
(s)


2′′, 6′′
131.1
(d)
130.9
(d)
131.0
(d)
128.8
(d)
130.2
(d)


3′′, 5′′
115.1
(d)
114.9
(d)
115.0
(d)
115.4
(d)
115.0
(d)


4′′
160.9
(s)
160.1
(s)
160.2
(s)
158.7
(s)
158.7
(s)


1′′′
65.0
(t)
64.9
(t)
65.1
(t)
64.1
(t)
64.8
(t)


2′′′
118.7
(d)
119.3
(d)
119.2
(d)
119.4
(d)
119.3
(d)


3′′′
139.1
(s)
138.6
(s)a)
138.9
(s)
138.3
(s)
138.4
(s)


4′′′
25.2
(q)
25.8
(q)
26.1
(q)
25.8
(q)
25.8
(q)


5′′′
18.2
(q)
18.2
(q)
18.5
(q)
18.1
(q)
18.2
(q)





a)Assignments may be interchangeable.






The structures of the new compounds were determined as follow: Compound 2 gave yellow needles, mp 110–111° C., and the molecular formula C19H23NO3 was assigned by HR-EIMS. The IR spectrum showed an imide carbonyl absorption at 1724 cm−1. The 13C-NMR spectrum showed signals of four methyl carbons, two methylene carbons, and one methine carbon in the aliphatic region, as well as one benzene ring, one olefinic group and two carbonyl carbons. The 1H-NMR spectrum showed the presence of an isobutyl moiety at δ 0 0.90, 2.06, and 2.51, a 3-methyl-2-butenyloxy moiety at δ 1.76, 1.81, 4.56, and 5.50, and a para-substituted benzene moiety at δ 6.95 and 7.50, which was further supported by 1H—1H COSY (cooler synchrotron) and HMQC (heteronuclear multiple quantum coherence) experiments. Long range correlations were observed by HMBC as shown in FIG. 1. On the basis of the molecular formula and the 13C-NMR spectrum, this compound was deduced to contain further CHNO atoms, including one more carbonyl carbon. Thus, this ambiguous part was speculated to be a maleimide group. This structure was then established to be 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrole-2,5-dione by X-ray analysis.
The molecular formula of compound 1 was assigned as C19H22O4 by HR-EIMS. The IR spectrum revealed a carbonyl absorption of acid anhydride at 1763 cm−1. The 1H-NMR spectrum of compound 1 was similar to that of compound 2, and showed the presence of an isobutyl moiety, a 3-methyl-2-butenyloxy moiety, and a para-substituted benzene ring. From the HMBC spectrum, compound 1 was demonstrated to have the same partial structure to compound 2 (FIG. 1), in which the presence of a maleic anhydride group was deduced on the basis of the molecular formula compound 1 was consequently determined as 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]furan-2,5-dione.
The molecular formula of compound 3 was assigned as C19H23NO4 by HR-EIMS. The IR spectrum showed a carbonyl absorption at 1717 cm−1, assignable to a hydroxy imide. The 1H- and 13C-NMR spectra were also similar to those of compounds 1 and 2, and showed the presence of an isobutyl moiety, a 3-methyl-2-butenyloxy moiety, and a para-substituted benzene ring. In the HMBC experiment, compound 3 was shown to have the same partial structure as compound 2 (FIG. 1). Compound 3 contains one more oxygen atom than compound 2, therefore, this compound was determined to be (3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl-1H-pyrrol-1-ol-2,5-dione.
Compounds 4 and 5 had the same Rf values and the same molecular formula by HR-EIMS (C19H25NO4, found 331.1747 and 331.1766, respectively), however, they could be separated by preparative HPLC. The IR spectrum of both compounds showed a hydroxy imide carbonyl absorption at 1715 cm−1. In the 1H- and 13C-NMR spectra, both compounds showed the presence of an isobutyl moiety, a 3-metyl-2-butenyloxy moiety, and a para-substituted benzene ring, but the isobutyl methylene protons displayed a multiplet and not a doublet as for compounds 1–3. The 1H-1H COSY spectrum indicated that this methylene group is attached to a —CH—CH— unit. The 13C-NMR spectra of compounds 4 and 5 exhibited two additional sp3 carbon signals, replacing two sp2 carbon signals observed for compounds 1–3. Therefore, compounds 4 and 5 were not N-hydroxy maleimides, but rather N-hydroxy succinimides, with stereocenters at positions C-3 and C-4 in the succinimide ring. Compounds 4 and 5 were determined to be trans and cis isomers, respectively, from the coupling constant between H-3 and H-4 (4.0 and 8.0 Hz for compounds 4 and 5, respectively). No NOE was observed between H-3 and H-4 in the NOESY (Nuclear Overhauser Effect Spectroscopy) spectrum of compound 4, while appreciable NOE was observed in that of compound 5. The optical rotations of compounds 4 and 5 showed +2.5° and +3.0°, respectively, while their CD spectra showed no Cotton effects at any wave length, suggesting that both compounds 4 and 5 are racemic mixtures. Resolution of these racemic mixtures by HPLC using a chiral column with several solvent systems was unsuccessful. At present, we cannot definitely conclude whether these compounds are optically active compounds or racemic mixtures. Thus, their relative structures were determined as 3R*,4S*- and 3R*,4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, respectively.
Isolation of these type of maleic and succinic acid derivatives from nature were second time followed by the report of Aquveque et al.
The cytotoxic activity of the chloroform extract and isolated compounds were investigated using LLC (Lewis lung carcinoma) cell line (Table 3). The chloroform extract showed moderate cytotoxic effects with an ED50 value of 26.7 μg/ml. Maleic compounds 1 and 4 had no cytotoxic activity, whereas compounds 2 and 3 were found to be cytotoxic to the LLC cell line with ED50 values lower than that of the chloroform extract.







TABLE 3







50% Growth Inhibition (ED50) Values of the


CHCl3 Extract and Compounds 1–4 from the mycelia


of Antrodia Camphorata against LLC Cell Line









ED50 (μg/ml)














CHCl3 extract
26.7



1
>20



2
3.6



3
7.5



4
>10



Adriamycina)
0.14







a)Positive control.






Tumor Assay of ACM (Antrodia camphorata Mycelia Powder)
A. Cell line


    
    
        Adherent cell
        MCF-7 human breast carcinoma
        HT-29: human colon adenocarcinoma
        KATO III: human stomach carcinoma
        SW480: human colon adenocarcinoma
        SW620: human colon adenocarcinoma
        HepG2: human liver carcinoma
    
    


Suspension cell:

    
    
        EL4: mice lymphoma

B. Samples

    
    


Compound 1, Compound 3, ACM EtOH Extract, ACM H2O Extract
C. Assay method
Calculate ED50 (50% inhibition of effective dose
Adherent cell: MTT (methyl thiazolyl tetrazolium) method; for MCF-7, HT-29, KATO II1, SW480, HepG2, cells are determined at 3 days. SW620 at 4 days
Suspension cell: Cell count method; EL4 cells count at 5 days
D. Result
Calculation:

y=m Ln(x)+b

EXAMPLE













X
Y

















0

0.97


10
ppm
0.941


30
ppm
0.6


100
ppm
0.331









Use value of X (10, 30, 50 ppm) and Y to get correlation curve

y=−0.2643Ln(x)+1.5321

ED50=exp[(0.97/2−1.5321)/(−0.2643)]

Sample Preparation and Sample Description

A. ACM (Antrodia Camphorata Mycelia Powder) H2O Extract

    
    
        1. Add 1 g of ACM into 40 ml of RO H2O in a 250 ml beaker, put the beaker in ultrasonic water bath for 20 min at room temperature
        2. Stir at 45° C. water bath for 45 min
        3. Place the beaker in ultrasonic water bath for another 20 min
        4. Centrifuge the sample at 3000 rpm for 15 min
        5. Collect supernatant and perform serial dilutions with media.
    
    


B. Determination of Sample Concentration

    
    
        1. Weigh an evaporating dish (W1)
        2. Add 10 ml of H2O extract sample in the evaporating dish
        3. Place the evaporating dish in the oven to remove water W2)

Sample weight/ml=(W2−W1)/10

    
    


C. ACM (Antrodia Camphorata Mycelia Powder) EtOH Extract

    
    
        1. Add 100 ml of 95% alcohol to 20 g ACM in a 500 ml beaker and stir for 10 min at room temperature
        2. Filter the suspension through Advantec # 1 filter paper, and collect the filtrate
        3. Concentrate the filtrate by rotary vacuum evaporator to remove alcohol.
    
    


D. Compound 1 Pure compound from ACM
E. Compound 3 Pure compound from ACM
MTT Assay Method


    
    
        1. Discard old media after cell proliferation, then wash cells once with phosphate-buffered saline (PBS) once
        2. Wash down the cells with trypsin-EDTA
        3. Centrifuge at 1200 rpm for 5 min, then discard supernatant
        4. Suspend the pellet with 10 ml medium
        5. Mix 100 μl cell suspension with 100 μl trypan blue to calculate viable cells
        6. Add 1*104 cells/100 μl medium in each well of the 96 well plate, incubate the plateCO2 incubater at 37° C. for 24 hrs
        7. Discard old medium, wash cells once with PBS
        8. Add 100 μl sample in each well, incubate the plate in CO2 incubater at 37° C.
        9. Wash cells once with PBS at 3rd, 4th and 5th days,
        10. Add 57 μl MTT (0.88 mg/ml) in each well
        11. After 4 hrs discard MTT and wash cells with PBS once
        12. Add 50 μl DMSO/Well
        13. Read at OD545 on Elisa reader

Cell Count Method (EL4 Cell Line)

        1. Discard old media after cell proliferation by centrifugation
        2. Resuspend the pellet with fresh medium
        3. Mix 100 μl cell suspension with 100 μl trypan blue to calculate viable cells
        4. Prepare different concentration of samples that contain 1*105 cell/ml sample
        5. Load 100 μl sample in each well of the 96 well plate, incubate the plate at 37° C. CO2 incubater
        6. Calculate viable cells at 3rd, 4th and 5th days
    
    












PBS



















NaCl
8
g



KCl
0.2
g



Na2HPO4
1.4
g



KH2PO4
0.2
g










Make volume to 1 L
PH 7.4










Result and Discussion











ED50 of ACM on Cell Lines

















KATO






Cell line
HepG2
HT-29
III
EL4
SW480
SW620
MCF-7
























Compound 1
21
ppm
52
ppm
38
ppm
3.5
ppm


15
ppm
6
ppm


Compound 3
35
ppm
42
ppm
69
ppm
2.6
ppm
20
ppm
27
ppm
0.02
ppm


ACM EtOH
32
ppm
52
ppm
156
ppm
2.6
ppm
71
ppm
4
ppm


Extract


ACM H2O
295
ppm
707
ppm


20
ppm
207
ppm
132
ppm
318
ppm


Extract









Deatiled test results as follows:

    Compound 3 of the invention: HepG2 (FIG. 4a), EL4 (FIG. 4b), HT-29 (FIG. 4c) and Kato III (FIG. 4d).
    ACM H2O Extract: HepG2 (FIG. 5a), SW620 (FIG. 5b) and EL4 (FIG. 5c).
    ACM EtOH Extract: HT-29 (FIG. 6a), SW480 (FIG. 6b), SW620 (FIG. 6c), EL4 (FIG. 6d), HepG2 (FIG. 6e) and Kato III (FIG. 6f).
    Compound 1 of the invention: MCF-7 (FIG. 7a), EL4 (FIG. 7b), HT-29 (FIG. 7c), SW620 (FIG. 7d) and HepG2 (FIG. 7e).

Given the above, it demonstrates that the compounds and ACM Extract of the invention have inhibition effect on various types of tumor cells.



Analysis of All New Compounds (1, 2 and 3) from ACM EtOH Extract by High Performance of Liquid Chromatography Method
Purpose: In order to measure the amount of all new compounds (1, 2 and 3) from ACM EtOH Extract, High Performance of Liquid Chromatography was employed as our routine quality control procedures.
Preparation for ACM EtOH Extract Sample


    1) By using digital balance precisely weight 20.000(g) of sample powders in a graduated media lab bottle with 100 mL of 95% alcohol, and do not screw the lid tightly on.
    2) Place above step of the sample bottle in ultrasonic water bath 10 minutes.
    3) Pour liquid samples to centrifuge tubes, and then place those samples in a centrifuge remove crude particle, under condition of 6500 rpm/5 minutes.
    4) Filter liquid layer with filter paper, advantec No. 1.
    5) Concentrate filtering solution by rotary vacuum evapotator until appear a thick, yellowish liquid, alcohol free.
    6) Repeat three times of step 1 to 5, and then collect all extract product (total ACM EtOH Extract=4.60 g). Calculate yield.

Application By Water HPLC, Model 2690:
    
        1) Column: Reverse Phase C18
        2) Mobile Phase MeOH, H2O, acetonitrile
        3) Injection vol 20 μL
        4) Detection: Photodiode Array Detector 996 on wavelength 254 nm
        5) Preparation 1.000 (g) ACM EtOH Extract sample in 10 mL of alcohol for HPLC analysis*:
    
    


Results: according to HPLC analysis, the extract product contains pure compound 1, 2, and 3 was showed in following Table 4










TABLE 4





Standard Name
Compound 1
Compound 2
Compound 3















For ingredients of three standard compounds:


weight 0.0100 (g) in 1 mL of alcohol










Concentration
0.01
0.01
0.01


(g/mL)


Peak Area
49,315,783
129,327,136
136,255,406


Retention time
134.8
124.3
119.8


(min)







*For ACM EtOH Extract sample:


weight 1,000 (g) in 10 mL of alcohol










Concentration
8.59 × 10−3
5.59 × 10−4
1.659 × 10−2


(g/mL)


Peak Area
42,374,766
7,226,937
226,102,223


Percentage
8.59
0.559
16.59


yield % (w/w)









Therefore, the total weight of compounds 1, 2 and 3 is 5.92% by weight in ACM sample.

Tests for ACM-EtOH Extract
Materials and Equipment
1. Test Substances and Dosing Pattern
Test substance was administered orally at an initial dose of 1000 mg/kg for all in vivo assays in a vehicle of 2% Tween 80. Time of observation for each assay was described in methods.
2. Animals
Male or female ICR mice, Wistar-Okamoto derived male spontaneously hypotensive rat (SHR), Wistar and Long Evans derived rats provided by MDS Pharma Services Taiwan Ltd. were used. Space allocation for animals was as folloes: 29×18×13 cm for 10 mice, 45×23×21 cm for 6 rats, and 45×23×21 cm for 3 guinea pigs. Mice and rats were housed in APECR cages. The immunocompetent C57BL/6J male mice, 6–8 weeks age, weighing 21±2 gm were also used in this study and provided by National Taiwan University Animal Center. The animals were housed in Individually Ventilated Cages Racks (IVC racks, 36 Mini Isolator System). Each cage was sterilized by autoclave and contained 5 mice (in 26.7×20.7×14 cm). All animal were maintained in a controlled temperature (21°–23° C.) and humidity (60%–70%) environment with 12 hour light dark cycles for at least one week in the laboratory prior to use. Free access to standard lab chow (LabDiet Rodent Diet and Guinea Pig Diet, PMI Nutrition International, USA) and tap water was granted.
3. Cell Line and Culture Media
The murine melanoma cell line, B16-F0 (ATCC CRL-6322), was purchased from American Type Culture Collection and Dulbecco's Modified Eagle's Medium (GIBCO, USA) was used as culture medium. The tumor cells were incubated in an atmosphere containing 5% CO2 at 37° C.
4. Chemicals
General:
Distilled Water (In-house), Dimethyl Sulfoxide (DMSO, Merck, Germany), Isotonic Sodium Chloride Solution (Sintong Chemical Industry Co. Ltd., R.O.C.), magnesium Sulfate (MgSO4.7H2O, Wako, Japan), Meclofenamate Sodium (Sigma, USA), Methylcellulose (Signa, USA), Sodium Hydroxide (NaOH, Wako, Japan), Phosphate Buffered Saline (Sigma, USA) and Tween 80 (Wako, Japan).
Reagents
Glicose-HA assay kit (Wako, Japan), Alanine aminotransferase (ALT) assay kit (Wako, Japan), Aspartate aminotransferase (AST) assay kit (Wako, Japan), T-Cholesterol-HA and HDL assay kit (Wako, Japan), Hemolynac 3 Hemolys (Nihon Koden, Japan), Isotonic 3 Diluent (Nihon Koden, Japan).
5. Equipment
General Use:
Animal Case (ShinTeh, R.O.C.), Beaker 250 ml and 1000 ml (Kinmax, USA), disposable syringe (1 ml, Top Corporation, Japan), Forceps stainless (klappencker, Germany), Mouse scale #Z-40 (Taconic, USA), needle for oral administration (Natsune, Japan), Needle Hypodermic 23 G×1′′ (Top Corporation, Japan), pH Meter (Suntex, USA), Rat scale 500 g±2 g (Chien-chun, ROC), syringe Glass 1 ml, 2 ml and 5 ml (Mitsuba, Japan), and Scissors Stainless (Klappencker, Germany).
Methods and Results:

    1. Cholinergic Agonism, Central/Peripheral (Lippmann W and Pugsley T A. Arch Int Pharmacodyn. 227:324, 1977)


Test substance was administered orally to a group of 3 Wistar derived male or female rats weighing 150±20 g. During the subsequent 30–60 minute period, the number of animals exhibiting more than 10 seconds of chewing behavior (mouth and/or tongue movements) measured cumulatively and the number of animals exhibiting salivation or exhibiting salivation were noted. Positive responses observed in 2 or more (≧2) of 3 rats indicates possible central cholinergic activity and peripheral cholinergic activity.







TABLE 5







Result of Cholinergic Agonism, Central/peripheral in Rats










Central
Peripheral














Treatment
Route
Dose
N
Chewing
Score
Salivation
Score


















Vehicle
PO
10
ml/kg
1
−

−







2
−

−






3
−
0/3
−
0/3


ACM-EtOH Extract
PO
1000
mg/kg
1
+

−






2
+

−






3
+
(3/3)
−
0/3




300
mg/kg
1
−

−






2
−

−






3
+
1/3
−
0/3


Arecoline-HBR
IP
30
mg/kg
1
+

+






2
+

+






3
+
(3/3)
+
(3/3)









Vehicle and test substances were administered orally (PO) while the positive reference compound was injected intraperitoneally (IP). During the subsequent 30–60 minute period, the number of animal exhibiting more than 10 seconds of chewing behavior (mouth and/or tongue movements) measured cumulatively or exhibiting salivation were noted. Positive responses observed in 2 or more (≧2) of 3 rats indicates possible cholinergic activity or peripheral cholinergic activity.

    2. Cardiovascular, Blood Pressure and Heart rate (SHR 0, 1, 2, 4 hrs) (Yen T T et al. Life Sci. 22: 359, 1978)


Groups of 3 Wistar-Okamoto derived male spontaneously hypertensive rats (SHR) weighting 250±20 g were used; the mean systolic blood pressure was 200±20 mmHg and heart rate 400±30 beats/min. Blood pressure and heart rate were recorded indirectly by tail cuff method in a temperature controlled environment (32±1° C.) before (0 time) and 1, 2 and 4 hours after oral administration of test substance or vehicle. A reduction in systolic pressure by 10 percent or more (≧10%), or decrease in heart rate by 20 percent or more (≧20%), at each measured time interval relative to 0 time, is considered significant.







TABLE 6







Result of Cardiovascular, Blood Pressure


(SHR 0, 1, 2, 4 Hours) in Rats









% Control (from 0 times)













Treatment
Route
Dose
N
1 Hour
2 Hours
4 Hours

















Vehicle
PO
10
ml/kg
1
100
96
90






2
97
100
91






3
90
92
92






Ave.
96
96
91


ACM-EtOH
PO
1000
mg/kg
1
78
85
71


Extract



2
86
89
80






3
89
89
89






Ave.
(84)
(88)
(80)


Clonidine
PO
0.1
mg/kg
1
71
67
71






2
95
86
88






3
72
85
69






Ave.
(79)
(79)
(76)
















TABLE 7







Result of Cardiovascular, Heart Rate (SHR 0, 1, 2, 4 Hours)









% Control (from 0 times)













Treatment
Route
Dose
N
1 Hour
2 Hours
4 Hours

















Vehicle
PO
10
ml/kg
1
87
100
99






2
116
103
107






3
108
104
121






Ave.
104
102
109


ACM-EtOH
PO
1000
mg/kg
1
98
93
95


Extract



2
81
100
88






3
83
78
92






Ave.
87
90
92


Clonidine
PO
0.1
mg/kg
1
62
97
112






2
84
87
104






3
68
86
78






Ave.
(71)
90
98









SHR with systolic blood pressure of 200±20 mmHg and heart rates of 400±50 mmHg Beats/min were used. Blood pressure was recorded indirectly vial tail cuff at 0 time (before) and 1, 2 and 4 hours after oral administration of vehicle or test substance. A reduction in blood pressure by 10 percent or more (≧10%), or decrease in heart rate by 20 percent or more (≧20%) at each measurement time point relative to 0 time, shown in parenthesis, is considered significant.













Vehicle
10 ml/kg 0 time 229 mmHg and 403 mmHg



beats/minute as 100%.


ACM-EtOH Extract
1000 mg/kg 0 time 223 mmHg and 452 mmHg



beats/minute as 100%.


Clonidine
0.1 mg/kg 0 time 228 mmHg and 379 mmHg



beats/minute as 100%
















TABLE 8







Result of Cardiovascular, Blood Pressure


(SHR 0, 1, 2, 4 Hours) in Rats









% Control (from 0 times)













Treatment
Route
Dose
N
1 Hour
2 Hours
4 Hours

















Vehicle
PO
10
ml/kg
1
94
97
97






2
88
97
94






3
94
97
103






Ave.
92
97
98


ACM-EtOH
PO
300
mg/kg
1
111
102
103


Extract



2
94
84
100






3
112
110
112






Ave.
106
99
105


Clonidine
PO
0.1
mg/kg
1
86
73
81






2
63
73
90






3
62
68
80






Ave.
(70)
(71)
(85)
















TABLE 9







Result of Cardiovascular, Heart Rate (SHR 0, 1, 2, 4 Hours)









% Control (from 0 times)













Treatment
Route
Dose
N
1 Hour
2 Hours
4 Hours

















Vehicle
PO
10
ml/kg
1
82
85
84






2
88
115
102






3
109
111
119






Ave.
93
104
102


ACM-EtOH
PO
300
mg/kg
1
97
96
92


Extract



2
105
108
98






3
85
96
82






Ave.
96
100
91


Clonidine
PO
0.1
mg/kg
1
77
85
102






2
78
78
100






3
62
94
104






Ave.
(72)
86
102









SHR with systolic blood pressure of 200±20 mmHg and heart rates of 400±50 mmHg Beats/min were used. Blood pressure was recorded indirectly vial tail cuff at time 0 (before) and 1, 2 and 4 hours after oral administration of vehicle or test substance. A reduction in blood pressure by 10 percent or more 10%), or decrease in heart rate by 20 percent or more (≧20%) at each measurement time point relative to 0 time, shown in parenthesis, is considered significant.













Vehicle
10 ml/kg 0 time 220 mmHg and 410 mmHg



beats/minute as 100%


ACM-EtOH Extract
300 mg/kg 0 time 205 mmHg and 446 mmHg



beats/minute as 100%


Clonidine
0.1 mg/kg 0 time 235 mmHg and 417 mmHg



beats/minute as 100%









    3. Cholesterol, Serum (Total HDL, total/HDL, Ratio), Diet-Induced (Schurr P E et al., Atherosclerosis Drug Discovery. Plenum, N.Y., pp. 215–229, 1976)


Groups of 5 ICR derived male mice weighing 22±2 g were kept on a high fat diet (g/100 g: coconut oil, 8; cholesterol, 1.0; cholic acid, 0.3; lard 2; standard chow 88.7) for 7 days to induce hypercholesterolemia. Test substance was administered orally on days 5, 6 and 7. After fasting overnight, serum was obtained from each mouse and assayed for total cholesterol (Total), high density lipoprotein (HDL) and percent change in Total/HDL. A decrease of 20 percent or more (≧20%) in serum Total or increase of 20 percent or more (≧20%) in serum HDL or decrease of 40% or more (≧40%) in the Total/HDL ratio relative to vehicle treated control animals is considered significant.







TABLE 10







Result of Cholesterol, (Total/HDL, Total/HDL Ratio), Diet-Induced in Mice











Total
HDL
Total/HDL
















Treatment
Route
Dose
N
Indiv.
% Dce
Indiv.
% Dce
Indiv
% Dce




















Vehicle
PO
10
ml/kg × 3
1
361

70

5.16







2
316

82

3.85






3
379

79

4.80






4
392

78

5.03






5
367

86

4.27






Ave.
363
—
79
—
4.59
—


ACM-EtOH
PO
1000
mg/kg × 3
1
420

117

3.95


Extract



2
327

115

2.84






3
332

104

3.19






4
363

98

3.70






5
294

117

2.51






Ave.
347
4
110
(39)
3.15
31



PO
300
mg/kg × 3
1
370

66

5.61






2
301

65

4.63






3
217

74

2.93






4
379

76

4.99






5
328

98

3.35






Ave.
319
12
76
−4
4.20
8


Benzafibrate
PO
100
mg/kg × 3
1
230

91

2.53






2
214

120

1.78






3
225

133

1.69






4
231

123

1.88






5
242

97

2.49






Ave.
228
(37)
113
(43)
2.02
(56)









Vehicle, test substance or reference positive compound was administered orally (PO) on days 5, 6 and 7 after being fed a high cholesterol diet. Twenty-four hours after the third dose, the overnight-fasted test animals were sacrificed for assessing serum total cholesterol (Total) and high density lipoprotein (HDL). Decrease of 20 percent or more (≧20%) in serum Total or increase of 20 percent or more (≧20%) in serum HDL or decrease of 40% or more (≧40%) in the Total/HDL ratio is considered significant.

    4. Hepatic Injury, D-Galactosamine (Wrobel J et al., J. Med Chem 41: 1084, 1998)


Groups of 5 Wistar derived male rats weighing 200±20 g were used. Each animal was treated with a single injection of D-galactosamine (500 mg/kg, IP) Test substance was administered orally at 0.5 hour before and 4 hours as well as 8 hours after D-galactosamine administration and animals were sacrificed 24 hours later. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by an optimized UV method with HITACHI automatic analyzer (model 7050). Reduction in ALT or AST activity by 30 percent or more (≧30%), relative to the vehicle treated control animal indicates significant protection.







TABLE 11







Result of Hepatic Injury, Galactosamine in Rats










Serum ALT (X ±
Serum AST (X ±



SEM)
SEM)



















Dec.

Dec.


Treatment
Route
Dose
N
U/L
%
U/L
%


















Vehicle
PO
10
mg/kg × 3
1
816

1628







2
1044

1716






3
652

888






4
656

828






5
644

956






X
762.4
—
1203.2
—






SEM
77.4

193.0


ACM-EtOH
PO
1000
mg/kg × 3
1
364

516


Extract



2
376

532






3
596

672






4
452

524






5
336

356






X
424.8
(44)
520.0
(57)






SEM
46.9

50.1




300
mg/kg × 3
1
460

852






2
656

880






3
640

876






4
752

1004






5
536

692






X
608.8
20
860.8
28






SEM
50.6

49.8


Guanine
PO
300
mg/kg × 3
1
508

656






2
532

912






3
412

776






4
436

652






5
636

1028






X
504.8
(34)
804.8
(33)






SEM
39.6

73.4









Test substance and vehicle administered orally at 0.5 hour before and 4, 8 hours after a single dose of galactosamine (500 mg/kg, IP). The rats were sacrificed 24 hours after galactosamine injection and the ALT and AST values were determined. A reduction of ≧30% in the ALT and AST relative to the vehicle group is considered significant.

    5. Inflammation, Carrageenan (Winter C A et al., Proc Soc Exp Biol Med. 111:544, 1962)


A group of 3 Long Evans derived male or female overnight fasted rats weighing 150±20 g was fasted overnight prior to study. Test substance was administered orally one hour before right hind paw received injection of carrageenan (0.1 ml of 1% suspension intraplantar). Hind paw edema, as a measure of inflammation, was recorded 3 hours after carrageenan administration using a plethysmometer with water cell (25 mm diameter). Reduction of hind paw edema by 30 percent or more (≧30%) indicates significant acute anti-inflammatory activity.







TABLE 12







Result of Inflammation, Carrageenan in Rats










Paw Volume (×0.01 ml)















Treatment
Route
Dose
N
R.P.
L.P.
Diff
% Inhibition


















Vehicle
PO
10
ml/kg
1
194
103
91







2
202
108
94






3
199
104
95






Ave.
198
105
93
—


ACM-EtOH
PO
1000
mg/kg
1
146
101
45


Extract



2
147
95
52






3
160
104
56






Ave.
151
100
51
(45)


Aspirin
PO
150
mg/kg
1
152
102
50






2
146
102
44






3
163
106
57






Ave.
154
103
50
(46)
















TABLE 13







Result of Inflammation, Carrageenan in Rats










Paw Volume (×0.01 ml)















Treatment
Route
Dose
N
R.P.
L.P.
Diff
% Inhibition


















Vehicle
PO
10
ml/kg
1
193
105
88







2
198
107
91






3
199
102
97






Ave.
197
105
92
—


ACM-EtOH
PO
300
mg/kg
1
195
104
91


Extract



2
187
103
84






3
196
103
93






Ave.
193
103
89
3


Aspirin
PO
150
mg/kg
1
146
103
43






2
149
101
48






3
169
104
65






Ave.
155
103
52
(43)









Vehicle or test substance was administered to overnight fasted rats one hour before right hindpaw (R.P.) injection of carrageenan (0.1 ml of 1% suspension intraplantar); the left hindpaw (L.P.) was not injected. Reduction of hindpaw edema by 30 percent or more (≧30%), shown in parenthesis, indicates significant acute anti-inflammatory activity.

    6. Tumor, Syngeneic, Melanoma, B16-F0 Cell (Farrugia C A and Groves M J. Anticancer Research 19: 1027–1032, 1999)


Groups of 5 immunocompetent (6–8 weeks old), pathogen-free (SPF) C57BL/6J male mice bred in an animal isolator (IVC racks) under specific pathogen free (SPF) condition were used. Viable B16-F0 murine melanoma cells (ATCC CRL-6322, 1.0×105 in 0.2 ml), syngeneic for C57BL/6J mice, were injected subcutaneously into dorsal side of experimental mice. Treatment begins 24 hours after tumor inoculation and test compound was administered daily by oral gavage for 21 days, or less when overt signs of toxicity are seen. The mice were monitored for body weight, tumor size and survival starting from day 1 to day 22. Moreover, the tested mice were monitored for survival till the end of the study on day 45.
Tumor weight (mg) was estimated according to the formula for a prolate ellipsoid: length (mm)×[width (mm)]2×0.5, assuming specific gravity to be one and π to be three. Tumor growth in compound treated animals was calculated as T/C (Treatment/Control)×100%; a value of T/C≦42% was considered significant in demonstrating antitumor activity.
The mean survival time of T/C (Treatment/Control) is ≧125% is also considered significant in demonstrating antitumor activity.







TABLE 14







Result of Tumor, Syngeneic, Melanoma B16-F0 Cell









Tumor Weight (mg) and % T/C,



Mean ± SEM
















Treatment
Route
Dose
N
Day 1.
T/C(%)
Day 8
T/C(%)
Day 11
T/C(%)




















Vehicle
PO
10
ml/kg × 21
1
0

0

60







2
0

39

298






3
0

0

49






4
0

54

541






5
0

21

117







0
100
23 ± 11
100  
213 ± 93 
100 


ACM-EtOH
PO
1000
mg/kg × 21
1
0

0

0


Extract



2
0

0

0






3
0

0

0






4
0

0

14






5
0

0

32







0
100
0 ± 0
0*
9 ± 6
 4*


Mitomycin
IP
2
mg/kg × 6
1
0

0

0






2
0

0

64






3
0

0

0






4
0

0

68






5
0

0

41







0
100
0 ± 0
0*
34 ± 15
16*
















TABLE 15







Result of Tumor, Syngeneic, Melanoma B16-F0 Cell









Tumor Weight (mg) and % T/C,



Mean ± SEM
















Treatment
Route
Dose
N
Day 15
T/C(%)
Day 18
T/C(%)
Day 22
T/C(%)




















Vehicle
PO
10
ml/kg × 21
1
211

746

2054







2
657

1597

2870






3
216

669

1419






4
835

2455

3688






5
240

726

1682







 432 ± 131
100 
1239 ± 349 
100 
2343 ± 416
100 


ACM-EtOH
PO
1000
mg/kg × 21
1
49

280

913


Extract



2
62

630

1545






3
388

1079

2560






4
148

435

1514






5
229

535

1637







175 ± 62
41*
592 ± 135
48 
1634 ± 265
70 


Mitomycin
IP
2
mg/kg × 6
1
36

256

437






2
136

849

1248






3
0

0

0






4
213

525

663






5
207

327

Died







119 ± 44
27*
391 ± 142
32*
 587 ± 260
25*









Vehicle and test substance were administered to test animals every day at 24 hours after tumor cells implantation for a total of 21 doses. Concurrently, the reference compound, mitomycin, was administered IP twice a week for a total of 6 doses. Tumor size was measured and recorded twice a week for a period of 22 days. Tumor growth inhibition was calculated as T/C (treatment/control)×100. A T/C value of ≦42% was considered significant in demonstrating antitumor activity.







TABLE 16







Result of Tumor, Syngeneic, Melanoma B16-F0 Cell









Body Weight (g), Mean ± SEM
















Treatment
Route
Dose
N
Day 1
Day 8
Day 11
Day 15
Day 18
Day 22




















Vehicle
PO
10
ml/kg × 21
1
21
20
20
21
22
25






2
22
22
21
22
26
30






3
21
21
20
20
22
21






4
21
20
20
20
21
24






5
22
21
20
19
20
23







21.4 ± 0.2
20.8 ± 0.4
20.2 ± 0.2
20.4 ± 0.5
22.2 ± 1.0
24.6 ± 1.5


ACM-EtOH
PO
1000
mg/kg × 21
1
20
21
21
22
22
23


Extract



2
20
19
19
21
22
24






3
20
18
18
19
21
26






4
21
20
19
21
20
21






5
20
20
21
22
23
25







20.2 ± 0.2
19.6 ± 0.5
19.6 ± 0.6
21.0 ± 0.5
21.6 ± 0.5
23.8 ± 0.9


Mitomycin
IP
2
mg/kg × 6
1
25
25
26
25
24
27






2
22
22
22
25
27
30






3
19
21
21
21
22
21






4
22
22
22
24
26
27






5
19
20
19
20
21
Died







21.4 ± 1.1
22.2 ± 0.8
22.0 ± 1.1
23.0 ± 1.0
24.0 ± 1.1
26.3 ± 1.9









Vehicle and test substance were administered to test animals every day at 24 hours after tumor cells implantation for a total of 21 doses. Concurrently, the reference compound. Tumor size was administered IP twice a week for a total of 6 doses. Tumor size was measured and recorded twice a week for a period of 22 days. The Student's t test was used to determine the siginifcant difference in the change of body weight between test compound and vehicle control group.







TABLE 17







Result of Tumor, Syngeneic, Melanoma B16-F0 Cell









Survival Time (day),



Mean ± SEM
















Days of
T/C


Treatment
Route
Dose
N
Post-treatment
(%)
















Vehicle
PO
10
ml/kg × 21
1
23







2
28






3
30






4
28






5
27







27.2 ± 1.2
100 


ACM-EtOH
PO
1000
mg/kg × 21
1
44


Extract



2
31






3
25






4
42






5
32







34.8 ± 3.6
128*


Mitomycin
IP
2
mg/kg × 6
1
 45a






2
28






3
 45a






4
30






5
22







34.0 ± 4.7
125*









    a: The animal did not die through day 45 and the survival day was served as 45 days.


The treated mice were monitored for survival through the end of the study on day 45 od the day when test animal died. The mean survival time of T/C (Treatment/Control)≧125% is also considered significant in demonstrating anti-tumor activity.
Discussion:
ACM-EtOH Extract, administered orally (PO), in accordance with in-house established criteria, caused significant activities in the following mouse and rat assays:
Central cholinergic agonism at 1000 mg/kg in rats; a minimum and non-significant agonism was seen a 300 mg/kg; no significant agonism or antagonism on peripheral cholinergic nerve was seen at 1000 mg/kg (Table 5)
Decrease in systolic blood pressure (16%, 12% and 20% at respective 1, 2 and 4 hours observation time points vs. 100% with 0 time) and associated moderate but non-significant decrease in heart rate at 1000 mg/kg in spontaneously hypertensive (SH) rats (Tables 6 and 7); dose of 300 mg/kg did not cause significant changes in systolic blood pressure nor the heart rate (Tables 8 and 9)
Increase in high density lipoprotein (HDL, 39% over vehicle control) at 1000 mg/kg in diet-induced mice (Table 10); the associate total cholesterol (Total) did not change significantly, while the HDL/total ratio was decreased to near significant 31%; dose of 300 mg/kg did not cause significant changes in Total, HDL and HDL/Total ratio (Table 10).
Hepatoprotection (44% decrease in ALT and 57% decrease in AST vs. vehicle control) from galactosamine induced hepatic injury in rats at 1000 mg/kg×3; moderate decrease of 20% in ALT and of 28% in AST at 300 mg/kg×3 was seen (Table 11)
Anti-inflammation (45% inhibition vs. vehicle control) versus carrageenan-induced paw edema in rats at 1000 mg/kg (Table 12); the lower level of 300 mg/kg did not demonstrate significant activity (3% inhibition vs. vehicle, Table 13)
Anti-tumor activity in syngeneic melanoma B16-F0 cell for C57BL/6J mice on day 8, 11 and 15 (Tables 14 and 15) as well as prolongation in animal survival time at 1000 mg/kg (Table 17); animal body weight did not change significantly (Table 16).
Tests for ACM, ACM-ETOH Extract and Compound 3 of the Invention Nine groups of ICR derived male mice (weighing 22±2 g) of 5 each were used.
Each animals was challenged with a single dose of carbon tetrachloride (CCl4, 0.1 ml/kg in 50% olive oil, PO). The test substance of ACM at doses 300 and 1000 mg/kg or compound 3 of the invention at doses 30, 100 and 300 mg/kg were administered orally at 30 minutes before and 4, 8 hours after CCl4 challenge; whereas ACM-ETOH Extract at 300 and 1000 mg/kg administered orally was pretreated one day (twice a day) and 30 minutes before and 4, 8 hours after carbon tetrachloride The animals were sacrificed 24 hours after CCl4. Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) levels were measured by optimized UV method using a HITACHI automatic analyzer (model 7050). A reduction of ALT or AST levels by 30 percent or greater (≧30%), relative to the vehicle group, indicating significant protection from hepatic injury.
Results







TABLE 18







Assay Hepatic Injury, Carbon Tetrachloride, in Mice










ALT
AST














Treatment
Route
Dose
N
U/L
Dec. %
U/L
Dec. %


















Vehicle (2%
PO
10
ml/kg × 3
1
3936

2056



Tween 80)



2
3456

1856






3
3712

1528






4
2560

1328






5
2968

1696






X
3326
0
1693
0






SEM
250

126


ACM
PO
1000
mg/kg × 3
1
1440

888






2
2720

1520






3
2272

1328






4
1272

792






5
1320

880






X
180.5
(46)
1082
(36)






SEM
292

144




300
mg/kg × 3
1
2336

1256






2
1552

1072






3
3720

1512






4
3816

2336






5
3952

2792






X
3075
8
1794
−6






SEM
480

330


ACM-EtOH
PO
1000
mg/kg × 5
1
1936

1232


Extract



2
1528

768






3
1896

1136






4
2752

1656






5
2472

1592






X
2117
(36)
1277
25






SEM
219

162




300
mg/kg × 5
1
1656

976






2
3536

1712






3
2328

1808






4
1736

1416






5
1792

888






X
2210
(34)
1360
20






SEM
352

187


Compound 3
PO
300
mg/kg × 3
1
1368

776






2
1576

896






3
1440

896






4
2728

1352






5
2720

1728






X
1966
(41)
1130
(33)






SEM
311

179




100
mg/kg × 3
1
3200

2256






2
4576

2976






3
2512

1536






4
2728

1552






5
3696

1600






X
3342
0
1984
−17






SEM
370

282




30
mg/kg × 3
1
4296

2136






2
3696

2288






3
2152

1096






4
2400

1792






5
4256

2496






X
3360
−1
1962
−16






SEM
457

245


Silymarin
PO
100
mg/kg × 3
1
2856

1296






2
1832

1152






3
1296

952






4
2792

1072






5
2728

1336






X
2301
(31)
1162
(31)






SEM
3136

71









Discussion:
ACM, ACM-EtOH Extract and compound 3 of the invention were evaluated for possible protective activity from hepatic injury induced by carbon tetrachloride in ICR mice. The test substance of ACM at doses 300 and 1000 mg/kg and compound 3 of the invention at doses 30, 100 and 300 mg/kg were administered orally to test animals 0.5 hour before and 4, 8 hours after CCl4 challenge. For ACM-EtOH Extract at 300 and 1000 mg/kg, 2 times (b.i.d.) of treatment (9:00 AM and 16:00 PM) were done 1 day before CCl4 and followed 0.5 hr before and 4, 8 hours after CCl4 challenge (5 dosing in total). The degree of hepatic injury was determined by increase in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels relative to the vehicle treated animals. ACM at 1000 mg/kg×3 and compound 3 of the invention at 300 mg/kg×3 caused a significant reduction of ALT (46% and 41%) and AST (36% and 33%) relative to the vehicle treated animals. Simultaneously, ACM-EtOH Extract at 300 and 1000 mg/kg×5 also caused significant reduction in ALT (36% and 34%) and AST (25% and 20%).
Concurrently tested silymarin (100 mg/kg×3, IP) showed significantly reduction of ALT (31%) and AST (31%) relative to the vehicle treated group.
It is concluded that ACM, ACM-EtOH Extract and compound 3 of the invention possess the ability of significant hepatoprotectant activity in a mouse CCl4 model.
While the invention has been described and exemplified in sufficient detail for those skilled in this art to make and use it, various alternatives, modifications, and improvements should be apparent without departing from the spirit and scope of the invention.
One skilled in the art readily appreciates that the present invention is well adapted to carry out the objects and obtain the ends and advantages mentioned, as well as those inherent therein. The cell lines, embryos, animals, and processes and methods for producing them are representative of preferred embodiments, are exemplary, and are not intended as limitations on the scope of the invention. Modifications therein and other uses will occur to those skilled in the art. These modifications are encompassed within the spirit of the invention and are defined by the scope of the claims.
It will be readily apparent to a person skilled in the art that varying substitutions and modifications may be made to the invention disclosed herein without departing from the scope and spirit of the invention.
All patents and publications mentioned in the specification are indicative of the levels of those of ordinary skill in the art to which the invention pertains. All patents and publications are herein incorporated by reference to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference.
The invention illustratively described herein suitably may be practiced in the absence of any element or elements, limitation or limitations, which are not specifically disclosed herein. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention that in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.
Other embodiments are set forth within the following claims.

## Claims

1. A compound having the formula




wherein X is N;
R1 is C2-10 alkenyloxy or C2-10 alkynyloxy;
R2 is C1-10 alkyl, C2-10 alkenyl or C2-10 alkynyl; and
R3 is H or hydroxyl.

2. The compound of claim 1, wherein
R1 is C2-6 alkenyloxy or C2-6 alkynyloxy.

3. The compound of claim 2, wherein
C2-6 alkenyloxy is substituted with C1-6 alkyl.

4. The compound of claim 1, wherein
R2 is isobutyl.

5. The compound of claim 1, which is
3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione,
3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-l-ol-2,5-dione,
3R*,4S*-1 -hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, or
3R*,4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione.

6. A composition comprising a compound having the formula




wherein X is N;
R1 is C2-10 alkenyloxy, or C2-10 alkynyloxy;
R2 is C1-10 alkyl, C2-10 alkenyl or C2-10 alkynyl; and R3is H or hydroxyl; and

a pharmaceutically acceptable excipient.

7. The composition of claim 6, wherein the compound is
3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5 -dione,
3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-l-ol-2,5 -dione,
3R*,4S*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, or
3R*,4R*-1--hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione.

8. The composition of claim 7, wherein the compound is 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione or 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-l-ol-2,5 -dione.

9. The composition of claim 6, which decreases systolic blood pressure or increases high density lipoprotein.

10. The composition of claim 6, which has central cholinergic agonism, hepatoprotection, anti-inflammation or anti-tumor activity wherein anti-tumor activity is exhibited against a tumor from cells or tissues selected from a group consisting of liver, intestine, bone, blood, lymph and breast.

11. The composition of claim 10, wherein the anti-tumor activity is exhibited against a tumor from cells or tissues selected from a group consisting of liver, intestine, bone, blood, lymph and breast.

12. The composition according to claim 6, further comprising:
a compound having the formula




wherein
R1is C2-10 alkenyloxy or C2-10 alkynyloxy; and
R2 is H, C1-10 alkyl, C2-10 alkenyl or C2-10 alkynyl.