BIOPESTICIDES REGISTRATION ACTION DOCUMENT

Methyl Jasmonate

 PC Code : 028100

U.S. Environmental Protection Agency

Office of Pesticide Programs

Biopesticides and Pollution Prevention Division

February 28, 2013
Methyl Jasmonate

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TABLE OF CONTENTS

I. EXECUTIVE SUMMARY:................................................................................................. 4

II. ACTIVE INGREDIENT OVERVIEW.............................................................................. 6

III. REGULATORY BACKGROUND.................................................................................... 6

A. Classification ........................................................................................................................ 6

B. Food Clearances/Tolerances............................................................................................... 6

IV. RISK ASSESSMENT ......................................................................................................... 7

A. Active Ingredient Characterization.................................................................................... 7

B. Human Health Assessment.................................................................................................. 7

1. Toxicology ......................................................................................................................................................7

2. Dose Response Assessment .........................................................................................................................10

 3. Drinking Water Exposure and Risk Characterization ......................................................... 10

4. Occupational, Residential, School and Day Care Exposure and Risk Characterization ......................11

5. Aggregate Exposure from Multiple Routes Including Dermal, Oral, and Inhalation...........................11

6. Cumulative Effects......................................................................................................................................11

7. Risk Characterization .................................................................................................................................11

C. ENVIRONMENTAL ASSESSMENT.............................................................................. 12

1. Ecological Hazards......................................................................................................................................12

2. Environmental Fate and Ground Water Data..........................................................................................12

3. Ecological Exposure and Risk Characterization ......................................................................................12

4. Endangered Species Assesment..................................................................................................................12

D. EFFICACY DATA............................................................................................................. 13

V. RISK MANAGEMENT DECISION................................................................................. 13

A. Determination of Eligibility for Registration .................................................................. 13

B. Regulatory Decision........................................................................................................... 13

VI. ACTIONS REQUIRED BY REGISTRANTS................................................................. 14

A. Reporting of Adverse Effects............................................................................................ 14

B. Reporting of Hypersensitivity Incidents.......................................................................... 14

VII. APPENDIX A. Data Requirements (40 CFR Part 158) ................................................. 14

VIII. APPENDIX B. Product Specific Information................................................................. 16

IX. APPENDIX C. References. ................................................................................................ 17

X. GLOSSARY....................................................................................... 17 
Methyl Jasmonate

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BIOPESTICIDES REGISTRATION ACTION DOCUMENT (BRAD) TEAM

Branch Chief

Linda A. Hollis, M.S.

Product Chemistry/Human Health Effects/Nontarget Organisms

Miachel Rexrode, Ph.D., Senior Biologist

Regulatory Action Leader

Chris Pfeifer
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I. EXECUTIVE SUMMARY:

Methyl jasmonate is a new biochemical pesticide active ingredient intended for use as a 

‘systemic acquired resistance’ (SAR) inducer on a variety of agricultural crops. It is applied as a 

seed treatment and an in-furrow soil treatment. Methyl jasmonate is naturally occurring and

elicits plant defense responses in vulnerable seedlings. It is the principal derivative of jasmonate, 

a hormone ubiquitous to most plants but particularly concentrated in jasmine and honeysuckle.

Jasmonates are known to trigger plant responses to a variety of stresses. Methyl jasmonate, in 

particular, is known to bolster plant defenses against extreme temperature changes and attacks by 

insects, fungi and bacteria. Methyl jasmonate is present in most fruits, is already a regular part 

of the human diet, and has a safe history of dietary exposure. Additionally, methyl jasmonate is 

an FAO/WHO approved flavoring agent, and is commonly consumed in processed foods with 

peach, apricot, berry, plum, tutti –fruitti, and tropical fruit flavors (JECFA, 2005). 

The Biopesticides and Pollution Prevention Division (BPPD) determined that the data and other 

information submitted for product chemistry and Tier I acute toxicity for methyl jasmonate

satisfy the current guideline requirements. Acceptable scientific information was submitted for 

the subchronic [90-day Oral (OCSPP 870.3100), 90-day Inhalation (OCSPP 870.3465) and 90-

day Dermal study (OCSPP 870.3250)], Developmental (OCSPP 870.3700), and Mutagenicity 

data requirements [In Vitro Mammalian Cell Assay (OCSPP 870.5300 and 5375) and Ames test 

(OCSPP 870.5100)] in lieu of guideline studies. BPPD determined that the information

submitted was acceptable based on methyl jasmonate’s toxicological and exposure profiles. 

Additionally, this compound is non-toxic to humans or to target pests, biodegrades readily, and 

intended application levels are below levels identified in commonly consumed foods, thus, the 

Agency concludes that there will be little or no risks from exposure to this active ingredient.

Although the data requirements were satisfied for the new active ingredient, methyl jasmonate, 

BPPD notes that should future intended end-use product (EP) applications result in different 

anticipated exposures to humans, these data requirements may need to be readdressed.

The Agency has accepted the waiver rationales for the following required ecotoxicity studies:

Acute Avian Dietary (OCSPP 850.2200); Fish Acute Toxicity (OCSPP 850.1075); Acute 

Aquatic Invertebrate (OCSPP 850.1010); Terrestrial Plant (Seedling Emergence) (OCSPP 

850.4100); Terrestrial Plant (Vegetative Vigor) (OCSPP 850.4150) and Nontarget Insect 

(OCSPP 880.4350). The rationales were based on a lack of exposure and toxicity. Foremost, 

methyl jasmonate is ubiquitous in the natural environment and has a non-toxic mode of action, 

with no demonstrable toxic effect to vertebrates or invertebrates. Second, due to the directed 

application methods proposed for this active ingredient (seed treatment and in-furrow drenching)

exposures are expected to be very low. These particular application methods greatly diminish 

exposure from drift and runoff. Additionally, a guideline study for Acute Oral Bobwhite Quail 

(OCSPP 850.2100) was found acceptable and confirmed non-guideline information 

demonstrating methyl jasmonate’s dietary non-toxicity. From the evidence presented by these 

data and rationale, the Agency concludes that there are no toxic risks to non-target organisms

from the proposed pesticidal use of methyl jasmonate. 

Based on the acute toxicity data for methyl jasmonate, the active ingredient is toxicity category 

III. EPA has not identified any toxic endpoints for nontarget mammals, birds, plants, aquatic, or 
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soil organisms. EPA has no concerns for any nontarget organisms exposed to methyl jasmonate

when used in accordance with approved label directions. Given that methyl jasmonate has 

negligible toxicity and presents little, if any, risk to nontarget organisms, EPA has concluded that 

it is in the best interest of the public to issue registrations for the Manufacturing-use Product 

(MP), Methyl Jasmonate Technical Grade (EPA File Symbol No. 71840-RN) and the End-use 

Product (EP) Tariss (EPA File Symbol No. 71840-RR), which contain this new active ingredient, 

methyl jasmonate.

BPPD has reviewed the data and information submitted in support of the request for registration

under Section 3(c)(5) of the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA). It has 

been determined that the data and information submitted adequately satisfy current biochemical 

pesticide guideline requirements (please refer to 40 CFR Subpart U § 158.2000). 

On October 1, 2009, EPA announced a new policy to provide a more meaningful opportunity for 

the public to participate on major registration decisions before they occur. According to this

policy, EPA provides a public comment period prior to making a registration decision for the 

following types of applications: new active ingredients, first food use, first outdoor use, first 

residential use; and any registration decisions for which the Agency believes there may be 

substantial public interest. 

Consistent with the policy of making registration actions more transparent, methyl jasmonate is 

subject to a 15-day comment period as a “new active ingredient.” EPA is affordig a comment 

period of 15 days because of methyl jasmonate’s negligible toxicity, the determination that 

methyl jasmonate presents low risk of any adverse effects to human health and the environment,

and the utility of this active ingredient. The notice for this comment period includes the draft 

Biopesticides Registration Action Document (BRAD) and draft product labels for the EPA File 

Symbol No. 71840-RN and EPA File Symbol No. 71840-RR), which contain this new active 

ingredient, methyl jasmonate. The docket identification (ID) number is EPA-HQ-OPP-2012-

0134. The Agency believes that based on the risk assessment and information submitted in 

support of the registration of the MP, Methyl Jasmonate Technical Grade (EPA File Symbol No. 

71840-RN), and the EP, Tariss (EPA File Symbol No. 71840-RR), containing methyl jasmonate, 

it is in the best interests of the public to issue the registration for Methyl Jasmonate Technical 

Grade and Tariss. The basis for this decision can be found in the risk assessment for methyl 

jasmonate, which is characterized in this BRAD. 
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II. ACTIVE INGREDIENT OVERVIEW

Common Name: Methyl Jasmonate

 Chemical Names: Cyclopentaneacetic acid, 3-oxo-2-(2-pentenyl)-,methyl ester

Trade & Other Names: Methyl Jasmonate Technical Grade, Jasmonate 

 CAS Registry Number: 1211-29-6

OPP Chemical Code: 028100

Type of Pesticide: Biochemical Pesticide - Systemic Acquired Resistance (SAR)

Inducer

III. REGULATORY BACKGROUND

On December 1, 2011, EPA received an application filed by Becker Underwood, Inc., 801 

Dayton Avenue, P.O. Box 667, Ames, IA, 50010, to register the products Methyl Jasmonate 

Technical Grade (EPA File Symbol No. 71840-RN) and Tariss (EPA File Symbol No. 71840-

RR), containing the new biochemical active ingredient, methyl jasmonate. A notice of receipt 

(NOR) of this application, allowing for a 30-day comment period, was published in the Federal 

Register on June 27, 2012 (77 FR 38285). No comments were received following this 

publication.

A. Classification

On April 13, 2010, the OPP Biochemical Classification Committee determined that methyl 

jasmonate is a naturally occurring plant hormone and SAR-inducer. This plant hormone’s nontoxic mode of action works by triggering plant immuno-defenses when seedlings are stressed.

These responses help protect the seedlings against insects, fungi, and bacteria. 

B. Food Clearances/Tolerances 

The applicant filed a petition (PP 1F7941) proposing to establish an exemption from the 

requirement of a tolerance for residues of methyl jasmonate in or on all food commodities. A 

notice of filing (NOF), allowing for a 30-day comment period, was published in the Federal 

Register on May 2, 2012 (77 FR 25954). No comments were received following this publication.

The Agency determined in its subsequent review of the active ingredient, methyl jasmonate, that 

the data submitted support an exemption from the requirement of a tolerance for residues of 

methyl jasmonate in and on all food commodities. Residues of proposed applications of methyl 

jasmonate are less than the concentrations found naturally in many fruit. Additionally, methyl 

jasmonate is an FAO/WHO-approved flavor agent allowed in greater concentrations than those 

resulting from the proposed application rates, and oral toxicity data demonstrate non-toxicity to 

mammals via oral routes of exposure. The Final Rule, supporting the exemption from the 
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Biopesticides Registration Action Document

requirement, is expected to be published immediately after the closing of the Public Participation 

Process.

IV. RISK ASSESSMENT

A. Active Ingredient Characterization

Methyl jasmonate is a new biochemical pesticide active ingredient intended for use as a SARinducer for seedlings. It is a derivative of a class of volatile organic compounds known as 

jasmonates, which are ubiquitous in most plants, but are particularly concentrated in jasmine and

honeysuckle. Jasmonates are known to act as plant hormones. In particular, methyl jasmonate is 

known to trigger plant responses to biotic and abiotic stresses and to regulate such processes as 

wound responses. Methyl jasmonate is produced as a reaction to stress and serves as a signal, in 

the form of a chemical vapor, to turn on natural plant defense pathways that increase plant

tolerance to temperature changes, as well as attack by insects. Signals from methyl jasmonate are

also known to lead to production of antifungal and/or antibacterial compounds.

Methyl jasmonate is extremely fragrant and carries a distinctive and desirable flavor. It is used as 

both a fragrance and a flavor agent. A joint FAO/WHO committee evaluated methyl jasmonate 

and deemed it non-toxic when used as a flavor agent (JECFA, 2005). Its use as a fragrance is 

permitted in candles, incense, soaps, shampoos, and lotions. It is commonly used as a flavor 

agent for processed foods with plum, peach and tropical fruit flavors. Jasmine extract, which 

contains significant concentrations of methyl jasmonate, is also used regularly in tea and as a 

spice for many Mid-Eastern dishes. 

The active ingredient methyl jasmonate approximates food grade methyl jasmonate. It has a high 

percentage of purity, and all impurities are typical of the compound and are considered to be 

non-toxic. Descriptions of the product formulation and production process, formation of 

impurities, and physical and chemical characteristics were examined by BPPD and found to 

confirm the purity and to be acceptable in meeting current biochemical pesticide guideline

standards. Additionally, the physical and chemical properties have all been well characterized. In 

sum, all product chemistry data requirements for registration of methyl jasmonate have been 

satisfied. 

For more information regarding product chemistry data requirements, please refer to Tables 1 

and 2 in Appendix A. 

B. Human Health Assessment

1. Toxicology

For acute toxicity data requirements, toxicity categories are assigned based on the hazard(s) 

identified from studies and/or information on file with the Agency. The active ingredient is 

classified into Toxicity Category I, II, III or IV where Toxicity Category I indicates the highest 

toxicity and Toxicity Category IV indicates the lowest toxicity. 
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Adequate mammalian toxicology data and information are available to support registration of

methyl jasmonate. All toxicology data requirements for methyl jasmonate have been satisfied.

a. Acute Toxicity

Acute toxicity testing is required to 1) determine systemic toxicity from acute exposure via the 

dermal, inhalation and oral routes, 2) determine irritant effects from exposure to the eyes, and 3) 

determine the potential for skin sensitization (allergic contact dermatitis). 

Tier I acute toxicity studies submitted and reviewed demonstrate that methyl jasmonate is

Toxicity Category III (slightly toxic) compound via acute oral route of exposure, based on 

testing at a limit dose of 5000 mg a.i./kg in rats, methyl jasmonate falls in Toxicity Category IV 

(not toxic) for acute dermal toxicity, acute inhalation toxicity, primary eye irritation and primary 

dermal irritations. Methyl jasmonate is not a dermal sensitizer. No toxicological endpoints were 

identified for this active ingredient. 

For more information regarding acute toxicity data requirements, refer to Table 3 in Appendix A. 

b. Subchronic Toxicity 

Subchronic data are required to determine a no-observed-effect-level (NOEL) and any toxic 

effects associated with repeated or continuous exposure to a test substance for a period of ninety 

days. 

The applicant submitted sufficient information on methyl jasmonate to satisfy requirements for 

Subchronic Toxicity testing [i.e. 90-day Oral (OCSPP 870.3100), 90-day Inhalation (OCSPP

870.3465) and 90-day Dermal (OCSPP 870.3250)]. The information submitted was found 

acceptable based on the toxicological and exposure profile of methyl jasmonate, summarized 

below.

i. Methyl jasmonate is a naturally occurring compound found in fruits and other plants and 

is already consumed in the human diet. This compound has a history of safe dietary 

exposure (Memorandum from Miachel Rexrode, Ph.D., July 19, 2012). 

ii. The proposed use pattern of this product results in exposure levels that are lower than the 

current estimated dietary exposure (Memorandum from Miachel Rexrode, Ph.D., July 19, 

2012). Oral exposure of children and adults to methyl jasmonate calculated from food 

consumption information from the U.S. EPA Exposure Factors Handbook (U.S. EPA, 

2009) were found to be less than the residues resulting from a maximum application.

iii. Methyl jasmonate was reviewed by the Joint FAO/WHO Expert Committee on Food 

Additives (JECFA, 2005) and was identified as a non-toxic flavoring agent. 

iv. Methyl jasmonate is not known or expected to be metabolized differently following 

exposure by the dermal route than the oral route and a literature search yielded no reports 

of subchronic dermal toxicity effects in rodents or humans from methyl jasmonate 

exposure (Memorandum from Miachel Rexrode, Ph.D., July 19, 2012). 

v. Prolonged dermal exposure is not expected because the product is not purposely applied 

to the skin and because handlers and applicators are required to wear appropriate PPE.
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vi. There is a long history of safe inhalation exposure to methyl jasmonate because it is a 

naturally occurring volatile component of common plants. The potential inhalation 

exposure concentration from use of products containing methyl jasmonate will be less 

than or equal to naturally occurring concentrations from crops and other plants

(Memorandum from Miachel Rexrode, Ph.D., July 19, 2012).

vii. Significant exposure to humans is not anticipated based on low application rates and 

rapid degradation in the environment (Memorandum from Miachel Rexrode, Ph.D., July 

19, 2012).

Please refer to Table 3 in Appendix A for more information on the subchronic data requirements.

 c. Developmental Toxicity and Mutagenicity

The applicant submitted sufficient information on methyl jasmonate to satisfy requirements for 

Developmental Toxicity and Mutagenicity [i.e. Prenatal Developmental Toxicity (OCSPP

870.3700), Bacterial Reverse Mutation (OCSPP 870.5100), In Vitro Mammalian Cell Gene 

Mutation (OCSPP 870.5300) and In Vitro Mammalian Chromosome Aberration (OCSPP 

870.5375)]. The information submitted was found acceptable based on the toxicological and 

exposure profile of methyl jasmonate, summarized below.

i. There is a long history of safe dietary exposure to methyl jasmonate because it naturally 

occurs in apples, strawberries and mangos (Lalel et al., 2003), fruits that are part of the 

normal diet.

ii. The potential oral exposure to methyl jasmonate from the proposed uses of methyl 

jasmonate is well below the average exposure for women of child-bearing age from the 

consumption of fruits that naturally contain methyl jasmonate, and also well below the 

JECFA human exposure threshold (Munro, 1999).

iii. Methyl jasmonate has been evaluated for safety by the FAO and determined to be 

metabolized to innocuous end-products that are eliminated in the urine (Lalel et al., 

2003).

iv. A literature search yields no reports of genotoxicity in laboratory studies on methyl 

jasmonate (Memorandum from Miachel Rexrode, Ph.D., July 19, 2012).

v. Significant exposure to child-bearing women is not anticipated based on low application 

rates, appropriate PPE requirements on the label, and rapid degradation in the 

environment. 

For more information regarding developmental and mutagenicity data requirements, refer to 

Table 3 in Appendix A. 
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d. Tier II and Tier III 

The biochemical pesticide Human Health Assessment data requirements for Tier II and Tier III 

were not required due to the nature of the active ingredient and its intended uses in EP products 

(SAR-inducing seed treatment and soil drench).

e. Effects on the Endocrine System

As required under FFDCA section 408(p), EPA has developed the Endocrine Disruptor 

Screening Program (EDSP) to determine whether certain substances (including pesticide active 

and other ingredients) may have an effect in humans or wildlife similar to an effect produced by 

a “naturally occurring estrogen, or other such endocrine effects as the Administrator may 

designate.” The EDSP employs a two-tiered approach to making the statutorily required 

determinations. Tier 1 consists of a battery of 11 screening assays to identify the potential of a 

chemical substance to interact with the estrogen, androgen, or thyroid (E, A, or T) hormonal 

systems. Chemicals that go through Tier 1 screening and are found to have the potential to 

interact with E, A, or T hormonal systems will proceed to the next stage of the EDSP where EPA 

will determine which, if any, of the Tier 2 tests are necessary based on the available data. Tier 2 

testing is designed to identify any adverse endocrine related effects caused by the substance, and 

establish a dose-response relationship between the dose and the E, A, or T effect.

Between October 2009 and February 2010, EPA issued test orders and data call-ins for the first 

group of 67 chemicals, which contains 58 pesticide active ingredients and nine inert ingredients. 

This list of chemicals was selected based on the potential for human exposure through pathways 

such as food and water, residential activity, and certain post-application agricultural scenarios. 

This list should not be construed as a list of known or likely endocrine disruptors.

Methyl jasmonate is not among the group of 58 pesticide active ingredients on the initial list to 

be screened under the EDSP. Under FFDCA section 408(p) the Agency must screen all pesticide 

chemicals. Accordingly, EPA anticipates issuing future EDSP test orders and data call-ins for all 

pesticide active ingredients. 

For further information on the status of the EDSP, the policies and procedures, the list of 67 

chemicals, the test guidelines and the Tier 1 screening battery, please visit our website: 

http://www.epa.gov/endo/.

2. Dose Response Assessment

Because no toxicological endpoints were identified for this active ingredient, a dose response 

assessment was not required. 

3. Drinking Water Exposure and Risk Characterization

No significant exposure via drinking water is expected when methyl jasmonate is used according 

to the product label directions. The active ingredient biodegrades rapidly in the environment, is 

applied at low application rates, and is not directly applied to water; therefore, residues of methyl 
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jasmonate are unlikely to accumulate in drinking water. In the unlikely event that exposure via 

drinking water does occur, the health risk would be expected to be minimal based on the low 

acute oral toxicity of jasmonate and the fact that methyl jasmonate is an edible compound that is 

ubiquitous in fruits and other edible plants.

4. Occupational, Residential, School and Day Care Exposure and Risk Characterization

a. Occupational Exposure and Risk Characterization

An occupational exposure assessment was not conducted for methyl jasmonate, and is not 

required. Appropriate PPE requirements on the label will mitigate any potential exposure to 

applicators and/or handlers. Additionally, no relevant toxicological endpoints have been 

identified. Based on the data and information available to the Agency, anticipated exposure is not 

likely to result in unreasonable risk to humans. 

b. Residential, School and Day Care Exposure and Risk Characterization

Exposure to methyl jasmonate will be minimal in residential, school, and day care areas, as the 

product containing this active ingredient is intended for use on agricultural crops. 

5. Aggregate Exposure from Multiple Routes Including Dermal, Oral, and Inhalation

There is reasonable certainty that no harm to the U.S. population will result from aggregate 

exposure to methyl jasmonate. This includes all exposures for which there is reliable 

information. The Agency arrived at this conclusion based on the lack of toxicity of this active 

ingredient and the already widespread exposure without any reported adverse effects on human 

health. The risks from aggregate exposure via oral, dermal and inhalation exposure are a 

compilation of three low-risk exposure scenarios and are negligible.

6. Cumulative Effects

Pursuant to FFDCA section 408(b)(2)(D)(v), EPA has considered available information 

concerning the cumulative effects of methyl jasmonate residues and other substances that have a 

common mechanism of toxicity. No toxicological endpoints have been established for exposure 

to methyl jasmonate; therefore, cumulative effects with other substances that share a common 

mechanism of toxicity are not expected. 

7. Risk Characterization

The Agency considered human exposure to methyl jasmonate in light of the relevant safety 

factors in FQPA and FIFRA. A determination has been made that no unreasonable adverse 

effects to the U.S. population in general, and to infants and children in particular, will result from 

the use of methyl jasmonate when label instructions are followed. 
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C. ENVIRONMENTAL ASSESSMENT

1. Ecological Hazards

The non-target toxicology information submitted by the applicant satisfies the non-target 

toxicology data requirements for methyl jasmonate and supports its registration.

The information submitted demonstrates that methyl jasmonate is practically nontoxic to birds, 

fish, insects and aquatic invertebrates; and is not phytotoxic. Jasmonate compounds are 

ubiquitous in plants and do not have a toxic mode of action. No toxic endpoints have been 

established for any of these non-target organisms.

For more information regarding nontarget organism toxicity data requirements, refer to Table 4

in Appendix A. 

2. Environmental Fate and Ground Water Data 

Environmental fate and groundwater data are not required at this time because the results of the 

nontarget organism toxicity assessment (Tier I data requirements) did not trigger these Tier II 

data requirements. 

3. Ecological Exposure and Risk Characterization

Jasmonate compounds, including methyl jasmonate, are ubiquitous to all plants and are not 

found to have toxic effects on vertebrates, invertebrates, or plants. Additionally, little to no 

exposure is expected for non-target organisms due to the directed application methods. It has 

been established that the potential exposure to birds from use of the seed treatment or soil 

drenching will be less than exposures from naturally occurring concentrations in crops and other 

plants. Further, any exposure from treated seeds to avian species and other seed eating organisms 

is low and not likely to cause toxic risk. With regard to aquatic organisms, exposure is 

anticipated to be even more unlikely because of the terrestrial application methods - seed 

treatment and in-furrow application - which eliminate potential exposure from drift and runoff. 

Finally, exposures to non-target plants are also not anticipated. And even if non-target plants 

were to be exposed, methyl jasmonate residues would not be expected to have a toxic effect 

since they are known bolster plant defenses. Little to no exposure is expected for non-target 

organisms; however, any incidental exposures are not anticipated to be of any concern as data 

demonstrate that methyl jasmonate is non-toxic to non-target organisms. 

4. Endangered Species Assessment

The Agency has not conducted a risk assessment that supports a complete endangered species 

determination. The ecological risk assessment planned during registration review will allow the 

Agency to determine whether methyl jasmonate’s use has “no effect” or “may effect” federally 

listed threatened or endangered species (listed species) or their designated critical habitats. When 

an assessment concludes that a pesticide’s use “may affect” a listed species or its designated 
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critical habitat, the Agency will consult with the U.S. Fish and Wildlife Service and/or National 

Marine Fisheries Services (the Services) as appropriate. 

D. EFFICACY DATA 

Product performance data must be developed for all pesticides to ensure that pesticide products 

will perform as intended and that unnecessary pesticide exposure to the environment will not 

occur as a result of the use of ineffective products. The Agency reserves the right to require, on a 

case-by- case basis, submission of efficacy data for any pesticide product registered or proposed 

for registration that is intended to be used to control a pest of significance public health 

importance, as defined in FIFRA section 28(d) and section 2(nn). For further guidance on 

product performance requirement, refer to Pesticide Registration Notice (PR) Notices 96-7, 

2002-1 and Explanation of Statutory Framework for Risk-Benefit Balancing for Public Health 

Pesticides (http://www.epa.gov/PR _Notices/pr1996-7.pdf) 

(http://www.ea.gov/PR_Notices/pr2002-1.pdf) and (http://www.epa.gov/pesticides/health/riskbenefit.htm).

The EP submitted with this new active ingredient did not list pests of significance public health 

importance or a public health pest as defined in FIFRA section 28(d) and section 2(nn). 

Therefore, product performance (efficacy) was not evaluated. 

V. Risk Management Decision

A. Determination of Eligibility for Registration 

Section 3(c)(5) of FIFRA provides for the registration of a new active ingredient if it is 

determined that: (A) its composition warrants proposed claims; (B) its labeling and other 

materials comply with the requirements of FIFRA; (C) it will perform its intended function 

without unreasonable adverse effects on the environment; and (D) when used in accordance with 

widespread and commonly recognized practice, it will not generally cause unreasonable adverse 

effects on the environment. 

The four criteria of the Eligibility Determination for Pesticidal Active Ingredients are satisfied by 

the science assessments supporting products containing the technical grade active ingredient, 

methyl jasmonate. Such products are not expected to cause unreasonable adverse effects. 

Therefore, methyl jasmonate, as a technical grade active ingredient, is eligible for registration for 

the labeled uses. 

B. Regulatory Decision

The data submitted fulfill the registration requirements of methyl jasmonate for use as a SARinducer. Refer to Appendix B for product-specific information.
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Conditional/Unconditional Registration

All data requirements are fulfilled, and EPA determined that an unconditional registration of 

methyl jasmonate is appropriate.

C. Environmental Justice

EPA seeks to achieve environmental justice—the fair treatment and meaningful involvement of 

all people regardless of race, color, national origin, or income—with respect to the development, 

implementation, and enforcement of environmental laws, regulations, and policies. At this time, 

EPA does not believe that use of methyl jasmonate pesticide products will cause harm or a 

disproportionate impact on at-risk communities. For additional information regarding 

environmental justice issues, please visit EPA’s website at

http://www.epa.gov/compliance/environmentaljustice/index.html.

VI. ACTIONS REQUIRED BY REGISTRANTS

EPA evaluated all data submitted in connection with the registration of the methyl jasmonate

pesticide products and determined that these data are sufficient to satisfy current registration data 

requirements. At this time, no additional data must be submitted to EPA for these particular 

products. For new uses and/or changes to existing uses, EPA may require additional data. 

Notwithstanding the information stated in the previous paragraph, it should be clearly understood 

that certain specific data are required to be reported to EPA as a requirement for maintaining the 

Federal registration for a pesticide product. A brief summary of these types of data are listed 

below. 

A. Reporting of Adverse Effects

Pursuant to FIFRA section 6(a)(2), reports of all incidents of adverse effects to the environment 

must be submitted to EPA.

B. Reporting of Hypersensitivity Incidents

Under the provisions of 40 CFR Part 158.2050(d), all incidents of hypersensitivity (including 

both suspected and confirmed incidents) must be reported to the Agency.

VII. Appendix A. Data Requirements (40 CFR Part 158-Subpart U)

*NOTE: Master Record Identification (MRID) numbers listed in the following tables are 

representative of supporting data/information for the original registration of the product 

containing this active ingredient. Subsequent to this registration, there may be additional MRIDs 

that support registration of other products containing this active ingredient. 
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TABLE 1. Acceptable Product Chemistry Data Requirements for Methyl Jasmonate (40 CFR § 158.2030)

MRID 48656602

OPPTS Guideline No. Study Results

830.1550

to

830.1670

Product identity;

Manufacturing process;

Discussion of formation of unintentional 

ingredients

Acceptable; Confidential Business Information 

(CBI)

830.1700 Analysis of samples Acceptable; CBI

830.1750 Certification of limits Limits listed in the CSF are adequate / acceptable.

830.1800 Analytical method Acceptable; CBI

TABLE 2. Acceptable Product Chemistry Data Requirements for Methyl Jasmonate (40 CFR § 158.2030)

From MRIDs 48656602-48656604

OCSPP 

No.

OCSPP Guideline 

Name

Observation Method

830.6302 Color TGAI: Pale yellow; EP: Colorless Visual inspection at room temp

830.6303 Physical state Liquid Visual inspection at room temp

830.6304 Odor Floral, true jasmine flower 

character

Olfactory inspection at room temp

830.6313 Stability to normal/elevated 

temps, metals and metal ions

TGAI should be fairly stable over 

long storage periods unless it is 

packaged in iron containers. The 

specific gravity of the samples 

stored at 540C showed a very 

slight increase (1.0227 at 250

C).The refractive index remained 

constant over the 14 day test 

period. EP: Test underway.

GC chromatograms. Refractive index 

and specific gravity were measured.

830.6315 Flammability >2300F Pensky Martens closed cup

830.6317 Storage stability (EP) Becker Underwood in-house 

study underway.

830.6319 Miscibility The product is miscible in water.

830.6320 Corrosion characteristics Becker Underwood in-house 

study underway.

830.7000 pH 5 - 9 at 22 0C Becker Underwood in-house method

830.7050 UV/Visible absorption max = 3.630 at 288.4 nm ; 

min=2.963 at 265.9 nm; max= 

4.063 at 237.3 nm

(0.3083 g/10 ml methanol)

UV/VIS Spectrometer

830.7100 Viscosity EP: 1 mPas @200 C In-house method

830.7200 Melting point/melting range 72.350 C QSAR EPISuite

830.7220 Boiling pt/boiling range TGAI: 313.620 C QSAR EPISuite

830.7300 Density/relative density/bulk 

density

1.02 g/ml at 20 0C Anton Parr Density meter, model 

DMA 4500

830.7550-7570 Partition coefficient NA 

830.7840 Water solubility EP water soluble. TGAI: 143.5 

mg/L 

QSAR EPISuite

830.7950 Vapor pressure Not required for end use product. 

TGAI: 0.000337 mm Hg

QSAR EPISuite
Methyl Jasmonate

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Table 3. Mammalian Toxicology Data Requirements for Methyl Jasmonate (40 CFR § 158.2050)

Guideline #

Test

Results/Toxicity Category MRIDs Study Conclusion

Acute Oral Toxicity

OCSPP 870.1100

LD50 = 3,129 mg/kg

III

48653901 Acceptable

Acute Dermal Toxicity

OCSPP 870.1200

LD50 > 5,050 mg/kg

IV

48653902 Acceptable 

Acute Inhalation Toxicity

OCSPP 870.1300

LC50 > 2.23 mg/L

IV

48653903 Acceptable 

Primary Eye Irritation

OCSPP 870.2400

Minimal eye irritation

IV

48653904 Acceptable

Primary Dermal Irritation

OCSPP 870.2500

Non-irritating

IV

48653905 Acceptable 

Dermal Sensitization

OCSPP 870.2600

Not a contact dermal sensitizer 48653906 Acceptable 

90 day Oral Toxicity-Rat

OCSPP 870.3100

No subchronic oral toxicity 48653908 Acceptable

90-day Dermal 

OCSPP 870.3250

No subchronic dermal toxicity 48653908 Acceptable

90-day Inhalation

OCSPP 870.3465

No subchronic inhalation 

toxicity

48653908 Acceptable

870.3700

Prenatal Developmental Toxicity Study - Rat

Non-mutagenic 48653908 Acceptable 

870.5100

Bacterial Reverse Mutation Test

Non-mutagenic 48653908 Acceptable

870.5375

In Vitro Mammalian Chromosome Aberration 

Tests 

Non-mutagenic 48653908 Acceptable

Table 4. Ecological Toxicity Data Requirements for Methyl Jasmonate (40 CFR § 158.2060)

Guideline #

Test

Results/Toxicity Category MRIDs Study Conclusion

Acute Avian Oral

OCSPP 850.2100

LD50 > 2,000 mg/kg 48653907 Acceptable

Acute Avian Dietary 

OCSPP 850.2200

Non-toxic to non-target birds 48653908 Acceptable

Fish Acute Toxicity

OCSPP 850.1075

Non-toxic to non-target fish 48653908 Acceptable 

Acute Aquatic Invertebrate

OCSPP 850.1010

Non-toxic to non-target 

aquatic invertebrates

48653908 Acceptable 

Terrestrial Plant (Seedling Emergence) 

OCSPP 850.4100

Non-toxic to non-target 

seedlings

48653908 Acceptable 

Terrestrial Plant (Vegetative Vigor) 

OCSPP 850.4150

Non-toxic to non-target 

plants

48653908 Acceptable 

Nontarget Insect 

OCSPP 880.4350

Non-toxic to non-target 

insects

48653908 Acceptable

VIII. Appendix B.

For product specific information, please refer to http://www.epa.gov/pesticides/pestlabels.
Methyl Jasmonate

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Biopesticides Registration Action Document

IX.Appendix C.

REVIEWS AND OTHER REFERENCES

Memorandum from Miachel Rexrode, Ph.D, Senior Biologist to Chris Pfeifer. Review of 

Request for New Registration for Methyl Jasmonate Technical and Product Registration Section 

3 for ScititarTM with Tolerance Exemption. U.S. Environmental Protection Agency Office of 

Pesticide Programs. July 19 2012. 

Memorandum from Miachel Rexrode, Ph.D, Senior Biologist to Chris Pfeifer. Review of 

Response to Deficiencies for New Registration for Methyl Jasmonate Technical and Product 

Registration Section 3 for ScititarTM with Tolerance Exemption. U.S. Environmental Protection 

Agency Office of Pesticide Programs. November 9 2012. 

Avanci NC, Luche DD, Goldman GH, Goldman MHS, 2010. Jasmonates are phytohormones 

with multiple functions, including plant defense and reproduction. Genetics and Molecular 

Research 9(1): 484-505.

JECFA, 2005. WHO Technical Report Series 928. Evaluation of Certain Food Additives

Sixty-third report of the Joint FAO/WHO Expert Committee on Food Additives. Section 4.1.5 

Monocyclic and bicyclic secondary alcohols, ketones and related esters. World Health

Organization.

Lalel HJD, Sing Z, Tan SC. 2003. The role of methyl jasmonate in mango ripening and

biosynthesis of aroma volatile compounds. J. Horticultural Science & Biotech 78 (4); 470-484

Meyer A, Miersch O, Buttner C, Dathe W, Sembdner G. 1984. Occurrence in plant growth

regulator jasmonic acid in plants. J. Plant Growth Regul. 3:1-8.

Mukkun L & Singh Z. 2009. Methyl jasmonate plays a role in fruit ripening of ‘Pajaro” 

strawberry through stimulation of ethylene biosynthesis. Scientia Horticulturae 123:5-10.

U.S. EPA, 2009. Exposure Factors Handbook: 2009 Update. Office of Research and

Development National Center for Environmental Assessment U.S. Environmental Protection

Agency. External Review Draft. EPA/600/R-09/052A.

Walters D, Cowley T, Mitchell A. 2002. Methyl jasmonate alters polyamine metabolism and 

induces systemic protection against powdery mildew infection in barley seedlings. J. Exptl 

Botany 53:747-756.

 

X. GLOSSARY OF ACRONYMS AND ABBREVIATIONS

a.i. active ingredient

BPPD Biopesticides and Pollution Prevention Division
Methyl Jasmonate

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Biopesticides Registration Action Document

BRAD Biopesticide Registration Action Document

bw body weight

CBI Confidential Business Information

CFR Code of Federal Regulations

cm3cubic centimeter

CSF Confidential Statement of Formula

°C degrees Celsius

EC50 median effective concentration. A statistically derived single concentration in 

environmental medium that can be expected to cause an effect in 50% of the test 

animals when administrated by the route indicated (inhalation). It is expressed 

as a concentration in air or water (e.g. mg/L).

EDSP Endocrine Disruptor Screening Program

EDSTAC Endocrine Disruptor Screening and Testing Advisory Committee

EP end-use product

EPA Environmental Protection Agency (the “Agency”)

FDA Food and Drug Administration

FFDCA Federal Food, Drug, and Cosmetic Act

FIFRA Federal Insecticide, Fungicide, and Rodenticide Act

FQPA Food Quality Protection Act

FR Federal Register

g gram

ha hectare

kg kilogram

Kow octanol-water partition coefficient

L liter

LC50 median lethal concentration. A statistically derived single concentration in air or 

water that can be expected to cause death in 50% of the test animals when 

administrated by the route indicated (inhalation and environment). It is 

expressed as a concentration in air or water (e.g. mg/L).

LD50 median lethal dose. A statistically derived single dose that can be expected 

to cause death in 50% of the test animals when administered by the route 

indicated (oral and dermal). It is expressed as a weight of 

substance per unit weight of animal (e.g., mg/kg).

MRID No. Master Record Identification Number

mg milligram

mPa millipascal

mL milliliter

MP manufacturing-use product

N/A not applicable

NE “No Effect”

NIOSH National Institute for Occupational Safety and Health

nm nanometer

NOEL no-observed-effect-level

NOF notice of filing

NOR notice of receipt
Methyl Jasmonate

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Biopesticides Registration Action Document

OPP Office of Pesticide Programs

OCSPP Office of Chemical Safety and Pollution Prevention

pa pascal

PPE personal protective equipment

PR Notice Pesticide Registration Notice

 TGAI technical grade of the active ingredient

 ug microgram

 USDA United States Department of Agriculture

 UV ultra-violet