Test Material: Propyzamide (Pronamide, RH-23315) 

MRID: 49300802 

Title: 

Method Validation Study for the Determination of Residues of 

Propyzamide and its Relevant Metabolites in Soil by Liquid 

Chromatography with Tandem Mass Spectrometry 

MRID: 49300801 

Title: 

Propyzamide - Independent Laboratory Validation of an Analytical 

Method for the Determination of Residues of Propyzamide and its 

Relevant Metabolites in Soil by Liquid Chromatography with Tandem 

Mass Spectrometry 

EPA PC Code: 101701 

OCSPP Guideline: 850.6100 

For CDM Smith 

 

Primary Reviewer: Lisa Muto Signature: 

 Date: 11/17/14 

Secondary Reviewer: Lynne Binari Signature: 

Date: 11/17/14 

 

QC/QA Manager: Joan Gaidos Signature: 

Date: 11/17/14 
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 2 of 10 

Analytical method for propyzamide and its transformation products RH-24580 and RH24644 in soil 

Reports: ECM: EPA MRID No.: 49300802. Hill, R.C., Q. Li and V. Dixit. 2013. 

Method Validation Study for the Determination of Residues of Propyzamide 

and its Relevant Metabolites in Soil by Liquid Chromatography with 

Tandem Mass Spectrometry. Dow AgroSciences Study No.: 110586. Report 

prepared, sponsored, and submitted by Dow AgroSciences LLC, 

Indianapolis, Indiana; 101 pages. Final report issued May 22, 2013. 

ILV: EPA MRID No. 49300801. Gemrot, F. 2013. Propyzamide - 

Independent Laboratory Validation of an Analytical Method for the 

Determination of Residues of Propyzamide and its Relevant Metabolites in 

Soil by Liquid Chromatography with Tandem Mass Spectrometry. EAS 

Study No.: S13-03290. Dow AgroSciences Study No.: 130751. Report 

prepared by Eurofina Agroscience Services Chem SAS, Vergèze, France, 

sponsored and submitted by Dow AgroSciences LLC, Indianapolis, Indiana; 

96 pages. Final report issued November 15, 2013. 

Document No.: MRIDs 49300802 & 49300801 

Guideline: 850.6100 

Statements: ECM: The study was conducted in accordance with USEPA FIFRA Good 

Laboratory Practice (GLP) standards (p. 3 of MRID 49300802). Signed and 

dated No Data Confidentiality, GLP, and Quality Assurance statements were 

provided (pp. 2-4). A statement of the authenticity of the study report was 

included with the quality assurance statement (p. 4). A signature page was 

also included (p. 5). 

ILV: The study was conducted in accordance with OECD GLP standards 

(2007; p. 3 of MRID 49300801). The test facility was compliant with French 

GLP (Appendix D, p. 96). Signed and dated No Data Confidentiality, GLP, 

and Quality Assurance statements were provided (pp. 2-4). A statement of 

the authenticity of the study report was not included; a signature page was 

included (p. 5). 

Classification: This analytical method is classified as supplemental. The study sponsor did not 

specify that the soil used in the ILV was either an equivalent, or more difficult, 

analytical sample condition as that used in the ECM. For all analytes in the ECM, 

performance data to validate the method at 10×LOQ were not reported. 

Sample recoveries were corrected in the ECM. In the ECM, no 

chromatograms were provided for three of the four soil matrices. Linearity 

data was not provided for one of the four soils in the ECM. 

PC Code: 101701 

Reviewer: Karen Milians, Ph.D., Chemist Signature: 

Date: 

Executive Summary
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 3 of 10 

This analytical method, Dow AgroSciences Study No. 110586, is designed for the quantitative 

determination of propyzamide and its transformation products RH-24644 and RH-24580 in soil 

at 0.005 μg/g using LC/MS/MS. For all analytes in the ECM, sample recoveries were corrected 

and performance data to validate the method at 10×LOQ were not reported. The LOQ is less than 

the lowest toxicological level of concern in soil. The ILV validated the method after one trial 

with no modifications or difficulties; samples fortified at 10×LOQ were included. The study 

sponsor did not specify that the soil used in the ILV (loamy sand soil) was either an equivalent, or more 

difficult, analytical sample condition as that used in the ECM (clay, loam, and silt loam soils). 

Table 1. Analytical Method Summary 

Analyte(s) 

by Pesticide

MRID

EPA 

Review Matrix Method Date

(dd/mm/yyyy) Registrant Analysis

Limit of 

Quantitation

(LOQ)

Environmental 

Chemistry 

Method

Independent 

Laboratory 

Validation

Propyzamide 

49300802 49300801 Soil 22/05/2013 

Dow 

AgroSciences 

LLC 

RH-24644 LC/MS/MS 0.005 μg/g 

RH-24580 

I. Principle of the Method 

Samples (5.0 ± 0.05 g) of soil were extracted twice with acetonitrile:water (80:20, v:v; 25 mL 

then 20 mL) by spinning via vortex for 10 seconds then shaking via reciprocating shaker for 30 

minutes at ca. 180 excursions/minute (p. 11; Appendix 1, p. 98 of MRID 49300802). Soil and 

extract were separated by centrifugation (5 minutes at 2000 rpm) and decanting. The volume of 

the combined extracts was adjusted to 50 mL using acetonitrile:water (80:20, v:v). After 

centrifugation (5 minutes at 4000 rpm), the combined extract was further diluted to a 5x dilution 

using acetonitrile:water (80:20, v:v) prior to analysis. 

Samples were analyzed for propyzamide (RH-23315) and its products RH-24644 and RH-24580 

by HPLC (Zorbax SB-C8, 4.6 mm x 75 mm, 3.5 μm column) using a mobile phase of (A) water 

with 0.1% formic acid and (B) acetonitrile with 0.1% formic acid [percent A:B at 0.01 min. 

75:25, 3.00-5.00 min. 10:90, 5:15-8.00 min. 75:25) with MS/MS-ESI (electrospray ionization; 

positive mode) detection and multiple reaction monitoring (MRM; p. 11; Appendix 1, pp. 100-

101 of MRID 49300802). Injection volume was 10 μL. Analytes were identified using two ion 

transitions; one for quantitation (Q) and one for confirmation (C; p. 21 of MRID 48599207). Ion 

transitions monitored were as follows: m/z 256.0→190.0 (Q) and m/z 258.0→192.0 (C) for 

propyzamide, m/z 274.1→145.1 (Q) and m/z 274.1→109.0 (C) for RH-24580 and m/z

256.0→145.0 (Q) and m/z 256.0→109.0 (C) for RH-24644. 

In the ILV, the method was performed exactly as written (pp. 19-20 of MRID 49300801). 

Approximate retention times for the analytes were reported as ca. 3.9 minutes for propyzamide, 

ca. 3.7 minutes for RH-24580 and ca. 4.7 minutes for RH-24644. The ILV study author noted 

the typing errors in the ECM which caused the extraction solvent and storage temperature to be 

“not in line with the validated method” (p. 26). The reviewer noted these typing errors as well. 
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 4 of 10 

The LOQ for all analytes was the same in the ECM and ILV at 0.005 μg/g (p. 18 of MRID 

49300802; Table 1, p. 30 of MRID 49300801). The LOD for all analytes was 0.0015 μg/g in the 

ECM and ILV. 

II. Recovery Findings 

ECM (MRID 49300802): Mean recoveries and relative standard deviations (RSDs) were within 

guidelines (mean 70-120%; RSD ≤20%) for analysis of propyzamide, RH-24580 and RH-24644 

in four soils (clay, loam and two silt loam) at fortification levels of 0.005 μg/g (LOQ) and 0.5 

μg/g (100×LOQ; corrected recoveries; Tables 3-14, pp. 24-37). Performance data (recovery 

results) from quantitation and confirmation ion analyses were comparable; recovery data was 

corrected for residues found in the control samples. Recoveries from samples fortified at 0.0015 

μg/g (LOD) ranged (matrices/quantification and confirmation ions combined) from 67-93% for 

propyzamide, 53-93% for RH-24580 and 67-87% for RH-24644 (DER Attachment 2). The soil 

matrices were well characterized (Table 2, p. 23). 

ILV (MRID 49300801): Mean recoveries and relative standard deviations (RSDs) were within 

guidelines (mean 70-120%; RSD ≤20%) for analysis of propyzamide, RH-24580 and RH-24644 

in loamy sand soil at fortification levels of 0.005 mg/kg (LOQ) and 0.050 mg/kg (10×LOQ; 

quantitation and confirmation ion analyses; Table 7, p. 36). Performance data (recovery results) 

from quantitation and confirmation ion analyses were comparable. Recoveries from samples 

fortified at 0.0015 mg/kg (LOD) ranged (quantification and confirmation ions combined) from 

107-117% for propyzamide, 42-81% for RH-24580 and 108-112% for RH-24644 (DER 

Attachment 2). The soil matrix was well characterized (soil origin was not reported; p. 16). The 

method was validated in the first trial (p. 25). 

Table 2. Initial Validation Method Recoveries for Propyzamide and Its Transformation 

Products in Soil1
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 5 of 10 

Analyte Fortification 

Level (μg/g)

Number 

of Tests

Recovery 

Range (%) 

Mean 

Recovery (%)

Standard 

Deviation (%) 

Relative Standard 

Deviation (%)

Clay (United Kingdom; Soil 001) 

Quantitation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 6 83-89 86 2.0 2.3 

0.5 6 81-92 87 4.4 5.1 

RH-24580 0.005 (LOQ) 6 84-93 87 3.7 4.3 

0.5 6 85-92 88 2.6 3.0 

RH-24644 0.005 (LOQ) 6 80-87 84 2.9 3.5 

0.5 6 84-90 87 2.9 3.4 

Confirmation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 6 78-97 88 7.6 8.7 

0.5 6 83-90 87 3.4 3.9 

RH-24580 0.005 (LOQ) 6 83-94 88 4.3 4.9 

0.5 6 81-91 88 3.7 4.2 

RH-24644 0.005 (LOQ) 6 84-88 86 2.0 2.4 

0.5 6 81-89 86 3.1 3.6 

Loam (United Kingdom; Soil 002) 

Quantitation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 6 81-96 86 6.2 7.1 

0.5 6 86-90 88 1.5 1.7 

RH-24580 0.005 (LOQ) 6 80-98 88 6.8 7.7 

0.5 6 86-93 90 2.3 2.5 

RH-24644 0.005 (LOQ) 6 81-88 85 2.5 2.9 

0.5 6 81-89 85 3.2 3.8 

Confirmation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 6 78-90 85 4.8 5.6 

0.5 6 80-90 86 4.2 4.9 

RH-24580 0.005 (LOQ) 6 78-87 83 3.5 4.2 

0.5 6 85-91 89 2.1 2.3 

RH-24644 0.005 (LOQ) 6 85-90 87 2.1 2.4 

0.5 6 84-90 87 2.0 2.3 

Silt loam (Germany; Soil 003)

Quantitation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 6 85-91 89 2.2 2.4 

0.5 6 79-87 84 3.3 3.9 

RH-24580 0.005 (LOQ) 6 85-95 89 3.6 4.0 

0.5 6 85-92 88 3.0 3.4 

RH-24644 0.005 (LOQ) 6 81-90 86 4.3 4.9 

0.5 6 80-86 83 2.2 2.6 

Confirmation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 6 74-90 84 5.5 6.6 

0.5 6 81-90 87 3.4 3.9 

RH-24580 0.005 (LOQ) 6 83-96 89 5.7 6.4 

0.5 6 82-91 87 3.7 4.2 

RH-24644 0.005 (LOQ) 6 83-91 87 3.4 4.0 

0.5 6 80-89 84 3.3 3.9 

Silt loam (United Kingdom; Soil 004)

Quantitation ion 

Propyzamide 0.005 (LOQ) 6 84-99 87 5.9 6.8 
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 6 of 10 

Analyte Fortification 

Level (μg/g)

Number 

of Tests

Recovery 

Range (%) 

Mean 

Recovery (%)

Standard 

Deviation (%) 

Relative Standard 

Deviation (%)

(RH-23315) 0.5 6 84-93 89 3.3 3.7 

RH-24580 0.005 (LOQ) 6 87-102 95 7.2 7.6 

0.5 6 89-99 93 3.9 4.2 

RH-24644 0.005 (LOQ) 6 80-97 90 6.5 7.2 

0.5 6 84-94 88 4.5 5.1 

Confirmation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 6 83-99 90 5.8 6.5 

0.5 6 89-94 91 2.1 2.4 

RH-24580 0.005 (LOQ) 6 84-90 87 2.4 2.7 

0.5 6 88-96 92 3.5 3.8 

RH-24644 0.005 (LOQ) 6 87-92 89 2.2 2.5 

0.5 6 83-90 87 3.0 3.4 

Data (corrected recovery results) were obtained from Tables 3-14, pp. 24-37 of MRID 49300802. 

1 Soil matrices were fully characterized (USDA texture classification; Table 2, p. 23 of MRID 49300802). 

2 Ion transitions monitored were as follows: m/z 256.0→190.0 (Q) and m/z 258.0→192.0 (C) for propyzamide, m/z

274.1→145.1 (Q) and m/z 274.1→109.0 (C) for RH-24580 and m/z 256.0→145.0 (Q) and m/z 256.0→109.0 (C) for 

RH-24644. 

Table 3. Independent Validation Method Recoveries for Propyzamide and Its 

Transformation Products in Loamy Sand Soil1 

Analyte 

Fortification 

Level 

(mg/kg)

Number 

of Tests

Recovery 

Range (%) 

Mean 

Recovery (%)

Standard 

Deviation (%) 

Relative Standard 

Deviation (%)

Quantitation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 5 89-104 97 7 7 

0.050 5 88-102 98 6 6 

RH-24580 0.005 (LOQ) 5 81-99 87 8 9 

0.050 5 88-97 95 4 4 

RH-24644 0.005 (LOQ) 5 102-121 109 7 7 

0.050 5 100-113 106 5 5 

Confirmation ion 

Propyzamide 

(RH-23315) 

0.005 (LOQ) 5 91-104 97 5 5 

0.050 5 90-104 98 5 5 

RH-24580 0.005 (LOQ) 5 88-106 97 7 7 

0.050 5 92-104 88 5 5 

RH-24644 0.005 (LOQ) 5 110-115 113 3 2 

0.050 5 101-114 109 5 5 

Data (results) were obtained from Table 7, p. 36 of MRID 49300801 and DER Attachment 2 (standard deviations). 

1 Soil matrix was fully characterized (USDA texture classification; soil origin not reported; p. 16 of MRID 

49300801). 

2 Ion transitions monitored were as follows: m/z 256.0→190.0 (Q) and m/z 258.0→192.0 (C) for propyzamide, m/z

274.1→145.1 (Q) and m/z 274.1→109.0 (C) for RH-24580 and m/z 256.0→145.0 (Q) and m/z 256.0→109.0 (C) for 

RH-24644. 
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 7 of 10 

III. Method Characteristics 

In the ECM and ILV, the LOQ and LOD values for propyzamide, RH-24580 and RH-24644 in 

soil were 0.005 μg/g and 0.0015 μg/g, respectively (p. 18 of MRID 49300802; p. 14; Table 1, p. 

30 of MRID 49300801). Following the method of Keith, L. H., et al. (see section V. References

below), the LOD and LOQ for determination of propyzamide and its transformation products in 

soil were calculated using the standard deviation from the 0.005 μg/g recovery results (p. 18 of 

MRID 49300802). The LOD was calculated as three times the standard deviation (3s), and the 

LOQ was calculated as ten times the standard deviation (10s) of the recovery results. The 

calculated values support the LOQ and LOD established for the method. 

Table 4. Method Characteristics 

Propyzamide RH-24580 RH-24644 

Limit of Quantitation (LOQ) 0.05 μg/g 

Limit of Detection (LOD) 0.015 μg/g

Linearity (Least squares 

calibration curve r and 

concentration range)1,2

Clay 

r = 0.998 (Q) 

r = 1.000 (C) 

(0.03-20 ng/mL) 

r = 0.999 (Q) 

r = 0.999 (C) 

(0.03-20 ng/mL) 

r = 0.994 (Q) 

r = 0.997 (C) 

(0.03-20 ng/mL) 

Loam 

r = 0.999 (Q) 

r = 0.999 (C) 

(0.03-20 ng/mL) 

r = 0.998 (Q) 

r = 0.999 (C) 

(0.03-20 ng/mL) 

r = 0.996 (Q) 

r = 0.998 (C) 

(0.03-20 ng/mL) 

Silt loam (-003) 

r = 0.998 (Q) 

r = 0.999 (C) 

(0.03-20 ng/mL) 

r = 0.999 (Q) 

r = 0.999 (C) 

(0.03-20 ng/mL) 

r = 0.998 (Q) 

r = 0.999 (C) 

(0.03-20 ng/mL) 

Silt loam (-004) 

Not reported 

Repeatable Yes 

Reproducible Yes 

(only loamy sand soil was tested in ILV) 

Specific Yes 

Data were obtained from p. 18; Tables 3-14, pp. 24-37; Tables 28-32, pp. 51-52 of MRID 49300802 and Table 7, p. 

36 of MRID 49300801. 

1 Reviewer-calculated r2

 values (matrices/ions combined) for the provided ECM r values were 0.9960-1.000 for 

propyzamide, 0.9960-0.9980 for RH-24580 and 0.9880-0.9980 for RH-24644 (DER Attachment 2). Reviewercalculated r2

 values for the provided ECM calibration curves (matrices/ions combined) were 0.9988-0.9999 for 

propyzamide, 0.9993-0.9999 for RH-24580 and 0.9942-0.9981 for RH-24644. The ILV study author reported 

calculated r2

 values (ions combined) of 0.9991 for propyzamide, 0.9997-0.9968 for RH-24580 and 0.9982-0.9991 

for RH-24644 (Figures 1-6, pp. 33-43 of MRID 49300801). Reviewer-calculated r2

 values for the provided ILV 

calibration curves (ions combined) were 0.9988-0.9999 for propyzamide, 0.9993-0.9999 for RH-24580 and 

0.9942-0.9981 for RH-24644 (DER Attachment 2). 

2 The concentration range 0.03 to 20 ng/mL was equivalent to sample concentrations of 0.0015 to 1.00 mg/kg (pp. 

23, 26 of MRID 49300801). 

Linearity is satisfactory when r2 ≥ 0.995. 
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 8 of 10 

IV. Method Deficiencies and Reviewer’s Comments 

1. The study sponsor did not specify that the soil used in the ILV (loamy sand soil, organic 

carbon 1.87 ± 0.20%; p. 16 of MRID 49300801) was either an equivalent, or more 

difficult, analytical sample condition as that used in the ECM (clay, loam, and silt loam 

soils, total organic carbon 2.1-4.4%; Table 2, p. 23 of MRID 49300802). 

2. In the ECM, no performance data was provided for 10×LOQ to validate the method. A 

validation sample set should consist of, at a minimum, a reagent blank, two unspiked 

matrix control samples, five matrix control samples spike at the LOQ, and five matrix 

control samples spiked at 10x LOQ for each analyte and matrix. 

3. In the ECM, sample recoveries were corrected for residues found in the control samples 

(Tables 3-14, pp. 24-37 of MRID 49300802). There was no indication that recoveries 

were corrected in the ILV (pp. 23-24; Tables 1-6, pp. of MRID 49300801). 

4. In the ECM, no chromatograms were provided for three of the four soil matrices. Only 

those of clay soil (Soil S01) were included; this set of chromatograms was complete, 

except for a reagent blank. 

5. In the ECM, a calibration curve and data were not provided for one of the four soils, the 

Silt Loam Soil -004; however, calibration data was provided for the stability (Soil S01 

clay only) and matrix effect studies (Tables 22-27, pp. 45-50 of MRID 49300802). Least 

squares calibration curve r values for both auxiliary studies were reported as 0.997-0.999 

for propyzamide, 0.997-1.000 for RH-24580 and 0.995-0.999 for RH-24644 (ions 

combined; Tables 28-32, pp. 51-52). 

6. A significant typographical error in the ECM was noted by the reviewer and the ILV 

study author (p. 11; Appendix 1, p. 98 of MRID 49300802). The extraction solvent 

[acetonitrile:water (80:20, v:v)] was erroneously reported as “acetonitrile:1.0N HCl 

(80:20, v:v)” on p. 11 of the study report. The correct extraction solvent was reported in 

Appendix 1. The ILV study author noted the typing errors in the ECM which caused the 

extraction solvent and storage temperature to be “not in line with the validated method” 

(p. 26 of MRID 49300801). This was not a modification of the ECM report. 

7. In the ILV, the reviewer noted that the LOD for the method was reported as 0.003 mg/kg 

on p. 18; however, due to data in the Tables and Figures section, the reviewer reported 

that the LOD of the ILV was the same as the ECM, 0.0015 μg/g (p. 18; Tables 1-6, pp. 

30-35; Figures 13-14, pp. 50-51; Figures 25-26, pp. 62-63; Figures 37-38, pp. 74-75 of 

MRID 49300801). 

 

8. A reagent blank chromatogram was not included in the ECM or ILV. 

9. The soil matrices were well characterized in the ECM and ILV (Table 2, p. 23 of MRID 

49300802; p. 16 of MRID 49300801). Soil sources were specified in the ECM, but not in 

the ILV.
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 9 of 10 

10. In the ECM, the stability of the analytes prepared in acetonitrile and stored at -20°C was 

investigated (p. 19 of MRID 49300802). It was determined that propyzamide (RH-23315) 

and RH-24580 were stable for up to 57 days, and RH-24644 was stable for up to 56 days. 

In addition, stability of the sample extracts was investigated (p. 19; Tables 15-20, pp. 38-

43 of MRID 49300802). Quantitation and confirmation ion analyses found RH-23315 

(85-93% recovered), RH-24580 (85-91%) and RH-24644 (84-88%) were stable in all soil 

extracts for 4 days at ca. 4°C (matrices combined; LOQ and 10×LOQ combined, n = 3 

for each; mean recoveries reported). 

In the ILV, stability of the sample extracts was also investigated (pp. 25-26; Table 9, p. 

37 of MRID 49300801). Quantitation ion analyses found RH-23315 (85% recovered), 

RH-24580 (91%) and RH-24644 (104%) were stable in the sample extracts for 7 days at 

4°C (LOQ, n = 5 for each; mean recoveries reported; confirmation ion not monitored). 

11. In the ECM, matrix effects were determined for each analyte/ion/matrix combination 

compared to neat solvent (p. 20; Table 21, p. 44 of MRID 49300802). Matrix effects 

were most notable for the clay soil; however, the results showed that the matrix effects 

were within ±20% and not significant. The chromatograms of control samples of the clay 

soil (Soil S01) showed small peaks at the retention times of the analytes, but these 

interferences were below the limit of detection, typically 10-20% of the LOQ at most 

(Tables 3-8, pp. 24-35; Figures 11-12, pp. 77-84 of MRID 49300802).

In the ILV, matrix effects were determined to be insignificant, as well (<20%; p. 25; 

Table 8, p. 36 of MRID 49300801). Chromatograms of the control samples showed no 

interferences (Figures 11-12, pp. 48-49; Figures 23-24, pp. 60-61; Figures 35-36, pp. 72-

73 of MRID 49300801). 

12. It was reported for the ILV that one set of fourteen samples required approximately five 

person hours to complete the preparation and extraction (p. 26 of MRID 49300801). 

Subsequent LC/MS analysis and evaluation required ca. 4 hours. The ILV study author 

reported that there was no communication with the method developers during the study; 

the only communication which occurred was the clarification of the extraction solvent 

prior to experiment initiation. 

V. References 

Keith, L. H.; Crummett, W.; Deegan, J., Jr.; Libby, R. A.; Taylor, J. K.; Wentler, G. Anal. Chem.

1983, 55, 2210-2218 (p. 21 of MRID 49300802). 

U.S. Environmental Protection Agency. 2012. Ecological Effects Test Guidelines, OCSPP 

850.6100, Environmental Chemistry Methods and Associated Independent Laboratory 

Validation. Office of Chemical Safety and Pollution Prevention, Washington, DC. EPA 

712-C-001. 

40 CFR Part 136. Appendix B. Definition and Procedure for the Determination of the Method 

Detection Limit-Revision 1.11, pp. 317-319. 
Propyzamide (PC 101701) MRIDs 49300802 (ECM)/ 49300801 (ILV) 

Page 10 of 10 

Attachment 1: Chemical Names and Structures 

Propyzamide (Kerb, pronamide, RH-23315, RH-3315, RH-315) 

 

IUPAC Name: 3,5-Dichloro-N-(1,1-dimethylprop-2-ynyl)benzamide 

CAS Name: 3,5-Dichloro-N-(1,1-dimethyl-2-propynyl)-benzamide 

CAS Number: 23950-58-5 

SMILES String: CC(C)(NC(=O)c1cc(Cl)cc(Cl)c1)C#C (EPI Suite, v3.12 SMILES).

RH-24644 (RH-4644) 

IUPAC Name: 3,5-Dichloro-N-(1,1,2-trimethylprop-2-enyl)benzamide 

CAS Name: 2-(3,5-Dichlorophenyl)-4,4-dimethyl-5-methylene oxozoline 

CAS Number: 29918-40-9 

SMILES String: Not reported. 

RH-24580 (RH-4580) 

IUPAC Name: 3,5-Dichloro-N-(1,1-dimethyl-2-oxopropyl)benzamide 

CAS Name: N-(1,1-Dimethyl acetonyl)-3,5-dichloro-benzamide 

3,5-Dichloro-N-(1,1-dimethyl-2-oxo-n-propyl)benzamide (Table 1, p. 22) 

CAS Number: 29918-41-0 

SMILES String: Not reported.