# Patent

## Title
Method for detecting fishy water odor from air conditioner, reproducing fishy water odor and preparing corresponding fishy water odor composition

## Abstract

A method is provided that identifies the compounds contributing to fishy water odor from an air conditioner, reproduces the fishy water odor, and prepares a corresponding fishy water odor composition. Through the analysis method of the present invention, the compounds contributing to the fishy water odor from an air conditioner are identified and quantified. The fishy water odor is reproduced from a combination of the compounds identified by the analysis method of the present invention. The reproduced fishy water odor provides significant data required for development of an apparatus and a method for removing specific odor.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims priority under 35 U.S.C. §119 to Korean Patent Application No. 10-2013-0045017, filed on Apr. 23, 2013 in the Korean Intellectual Property Office, the disclosure of which is incorporated herein by reference in its entirety.
BACKGROUND
(a) Technical Field
The present invention relates to a method for detecting a combination of compounds contributing to fishy water odor from an air conditioner, a method for reproducing the fishy water odor and preparing a corresponding fishy water odor composition.
(b) Background Art
Clean air is an essential element for maintaining human health and well-being. Two important factors that lead to unsatisfactory interior air quality in an airtight building are the building itself producing a substantial amount of air pollutants that need to be removed or diluted; and odor generated as a result of human activities.
An air-cooling system lowers interior temperature and optimizes interior environment through air conditioning which changes air temperature, humidity, flow and cleanliness to more favorable conditions. Increasingly, air-cooling systems are being used to improve the standard of living. Although the air-cooling systems have been improved functionally over time, problems remain to be solved in terms of interior air quality. In the past, lowering interior temperature was viewed as one of the most fundamental and important functions of the air-cooling system. However, currently, health-related aspects such as interior air quality and odor may also be regarded as important functions of air-cooling systems. In particular, complaints regarding interior air quality include offensive odor such as malodor, foul odor, foot odor, and the like. To solve the odor problem, it may be necessary to analyze the odor-causing substances and understand the fundamental cause of the odor.
Although it is known that metabolites produced by fungi and bacteria are a cause of odor from an air conditioner, it is not clearly known what metabolites are produced and in what amount by the fungi and bacteria. Additionally, since it is unclear specifically what compounds cause the offensive odor, may be is necessary to understand the type of compounds that contribute to the fishy water odor from the air conditioner.
SUMMARY
Numerous complaints are commonly made regarding various offensive odors from an air conditioner (e.g., more than 20 types including musty odor). The present invention discloses a method for identifying the compounds contributing to fishy water odor from an air conditioner, collecting offensive-smelling gas from a vehicle, and developing a method for artificially reproducing the fishy water odor.
The present invention provides a method for detecting the compounds contributing to fishy water odor from an air conditioner from among various offensive odors emitted from the air conditioner.
The present invention also provides a method for identifying the compounds contributing to fishy water odor from an air conditioner from among various offensive odors emitted from the air conditioner and artificially reproducing the fishy water odor using the identified chemical compounds. Additionally, the present invention provides a method of preparing a corresponding fishy water odor composition from the detected fishy water odor. The fishy water odor composition may also be applicable to any applications where fishy water odor is emitted, in addition to the air conditioner.
In an aspect, the present invention provides a fishy water odor composition from an air conditioner including two or more compounds selected from a group consisting of: ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.
In another aspect, the present invention provides a fishy water odor composition from an air conditioner including ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.
In yet another aspect, the present invention provides a method for analyzing the compounds contributing to fishy water odor from an air conditioner, including the steps of:

    
    
        (i) collecting a gas emitted from an air conditioner; and
        (ii) analyzing the components of the gas.
    
    


In another aspect, the present invention provides a method for preparing a corresponding fishy water odor composition from a detected fishy water odor, including mixing two or more compounds selected from a group consisting of ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.



BRIEF DESCRIPTION OF THE DRAWING
The above and other objects, features and advantages of the present invention will now be described in detail with reference to exemplary embodiments thereof illustrated in the accompanying drawing which is given hereinbelow by way of illustration only, and thus are not limitative of the invention, and wherein:
FIG. 1 is an exemplary flow chart describing a method for analyzing the compounds contributing to fishy water odor according to an exemplary embodiment of the present invention.



DETAILED DESCRIPTION
It is understood that the term “vehicle” or “vehicular” or other similar term as used herein is inclusive of motor vehicles in general such as passenger automobiles including sports utility vehicles (SUV), buses, trucks, various commercial vehicles, watercraft including a variety of boats and ships, aircraft, and the like, and includes hybrid vehicles, electric vehicles, combustion, plug-in hybrid electric vehicles, hydrogen-powered vehicles and other alternative fuel vehicles (e.g., fuels derived from resources other than petroleum).
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms “a”, “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms “comprises” and/or “comprising,” when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. As used herein, the term “and/or” includes any and all combinations of one or more of the associated listed items.
Unless specifically stated or obvious from context, as used herein, the term “about” is understood as within a range of normal tolerance in the art, for example within 2 standard deviations of the mean. “About” can be understood as within 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.1%, 0.05%, or 0.01% of the stated value. Unless otherwise clear from the context, all numerical values provided herein are modified by the term “about.”
Hereinafter, reference will now be made in detail to various exemplary embodiments of the present invention, examples of which are illustrated in the accompanying drawings and described below. While the invention will be described in conjunction with exemplary embodiments, it will be understood that the present description is not intended to limit the invention to those exemplary embodiments. On the contrary, the invention is intended to cover not only the exemplary embodiments, but also various alternatives, modifications, equivalents and other embodiments, which may be included within the spirit and scope of the invention as defined by the accompanying claims.
In an aspect, the present invention provides a fishy water odor composition from an air conditioner including two or more compounds selected from a group consisting of: ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane. More specifically, the present invention provides a fishy water odor composition from an air conditioner including ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.
The fishy water odor composition from an air conditioner may include: about 0.08-0.20 ppb of ammonia; about 10.00-12.00 ppb of acetaldehyde; about 40.00-45.00 ppb of toluene; about 14.00-18.00 ppb of m/p-xylene; about 8.00-12.00 ppb of o-xylene; about 1.00-4.00 ppb of methyl isobutyl ketone; about 8.00-13.00 ppb of butyl acetate; about 15.00-19.00 ppb of butanol; about 0.10-3.00 ppb of hexanal; about 0.10-3.00 ppb of decanol; about 8.00-13.00 ppb of 1,3,5-trimethylbenzene; about 0.10-3.00 ppb of acetone; about 38.00-42.00 ppb of dodecane; about 8.00-11.00 ppb of 2-butoxyethanol; about 0.10-4.00 ppb of 2-methoxyethanol; about 3.00-7.00 ppb of ethylbenzene; about 3.00-6.00 ppb of hexane; about 3.00-6.00 ppb of nonane; about 0.01-1.00 ppb of n-octane; and about 2.00-5.00 ppb of n-undecane.
In another aspect, the present invention provides a method for analyzing the compounds contributing to fishy water odor from an air conditioner, including the steps of:

    
    
        (i) collecting a gas emitted from an air conditioner; and
        (ii) analyzing the components of the gas.
    
    


In another aspect, the present invention provides a method for analyzing the compounds contributing to fishy water odor from an air conditioner and reproducing the corresponding fishy water odor, including: evaluating offensive odor; collecting a gas emitted from an air conditioner; analyzing the components of the collected gas; classifying the components; reproducing compositions; performing a multiple test (for at least 5 panelists); selecting the representative composition; and completing the reproduction of the fishy water odor composition.
FIG. 1 is an exemplary flow chart describing the method for analyzing the compounds contributing to fishy water odor and reproducing the fishy water odor. The fishy water odor from an air conditioner of the present invention may be from an air conditioner in an environment including a building, a vehicle, a van, a bus, and the like.
In an exemplary embodiment of the present invention, the gas in (ii) may include ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane. In addition, the concentration of the ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane that contribute to the fishy water odor may be measured. The analysis of the components in (ii) may be performed by gas chromatography/mass spectrometry (GC/MS), gas chromatography with atomic emission detector (GC/AED), gas chromatography/flame ionization detection/olfactometry (GC/FID/olfactometry) or high-performance liquid chromatography (HPLC), but is not necessarily limited thereto.
Representative examples of the analysis method of the present invention are described hereinbelow. However, the analysis method of the present invention is not limited thereto.
Gas Chromatography
In gas-solid chromatography (GSC), an adsorbent solid powder may be used as the stationary phase. In addition, in gas-liquid chromatography (GLC), a liquid stationary phase coated on a solid support may be used.
A carrier gas maintained at a constant flow rate may be supplied from a sample injection device into a separation column via a pretreatment apparatus and discharged after passing through a detector. The pretreatment apparatus, the sample injection device, the column and the detector may be maintained at predetermined temperatures.
When a gas or a liquid is introduced into the sample injection device, the gas may be carried into the column by the carrier gas and the liquid may be carried into the column by the carrier gas after being heated and evaporated. The components of the sample may be separated in the separation column based on difference in absorption or solubility and may sequentially pass through a mass analyzer disposed at the outlet of the separation column.
The time between the injection of the sample into the separation column until a peak occurrence as a result of detection of a specific component included therein may be called retention time, and the retention time multiplied by the flow rate of the carrier gas may be called retention volume. Qualitative analysis may be performed on the process since the values of the retention time and volume may be different for different components under given experimental conditions. Furthermore, quantitative analysis may be conducted since the peak area or height may be related to the amount of the corresponding present.
Electron ionization is a conventionally used ionization technique. Neutral sample molecules in gas state are bombarded with high-speed electrons to detach electrons and form molecular ions (cations, M+). The minimum energy required to produce the molecular ion (M+) from the neutral molecule (M) may be called ionization energy (IE). The ionization energy of an organic compound is 8-12 eV (800-1200 kJ/mol−1).

M+e−→M++2e−

Among the produced molecular ions, those with high internal energy may be fragmented to form fragment ions. To prevent ion formation as a result of reaction between the produced ion and the neutral molecule, the pressure inside the ionization source should be maintained at 10−5 torr or lower.
Electron beams emitted from a filament may be accelerated to 70 eV to obtain standard mass spectrums since they provide high ionization efficiency with little change in mass spectrum. The mass spectrum is the recording of the mass-to-charge ratio (m/z) of the molecular ions and the fragment ions. The mass spectrum of the unknown sample may be compared with the stored standard mass spectrums to identify the substance.
Electric fields and magnetic fields may be utilized alone or in combination to separate the ions according to their mass-to-charge ratio. A sector field analyzer, a quadruple mass analyzer, an ion trap, a time-of-flight analyzer, and the like may be used as a mass analyzer.
High-Performance Liquid Chromatography (HPLC)
The HPLC method may be used to separate nonvolatile substances which may be difficult to analyze by gas chromatography based on their difference in physicochemical interactions with a stationary phase and a liquid mobile phase. This method is used for qualitative and quantitative analysis of aldehydes in the air. Additionally, in HPLC, the target substances may be separated in the separation column based on their difference in reactivity with the stationary phase and the mobile phase.
When HPLC is used for analysis of aldehydes existing in the air, a separation column in which a nonpolar stationary phase is chemically bonded to a support may be used. Separation may be achieved depending on difference in reactivity and solubility for the mobile phase and the stationary phase. In general, the method wherein a column containing a nonpolar stationary phase is used and a relatively polar sample eluent is used to separate target substances may be called reversed-phase HPLC.
The material of the tubing in the HPLC method may be stainless steel, polytetrafluoroethylene (PTFE), polyether ether ketone (PEEK), glass, or a similar materials. Stainless steel may be advantageous due to being resistant to oxidation and corrosion. However, acid may cause damage and contamination to the tubing. Thus, when stainless steel or the like is used, the tubing should be washed with distilled water after use.
Often, an HPLC detector, capable of measuring absorption in the UV-visible (ultraviolet) region may be used. When incident light of a particular wavelength is emitted from a light source on the sample in the cell of the UV-visible detector, it may be absorbed by the sample. The detector may generate an electrical signal corresponding to the light absorbance, thus allowing quantitative analysis of the sample.
Hereinafter, the method for detecting the components contributing to fishy water odor according to the present invention is described. However, the application of the method is not limited to the described components.
Detection of Ammonia
The concentration of ammonia in the air may be measured as follows. After adding a phenol-sodium nitroprusside solution and a sodium hypochlorite solution to a sample solution to be analyzed, ammonia may be analyzed by measuring absorbance of indophenols formed from reaction with ammonium ion.
Detection of Methyl Mercaptan, Hydrogen Sulfide, Dimethyl Sulfide and Dimethyl Disulfide
The concentration of the sulfur compounds in the air may be measured as follows. After sampling using a sample bag, analysis may be performed by cold trap-capillary GC and cold trap-packed column GC.
Cold Trap-Capillary GC and Cold Trap-Packed Column GC
The sulfur compound sample collected in the sample bag may be concentrated in a cold trap device (which may be maintained at about −183° C. or below using a refrigerant) and may be analyzed by GC after desorption. The measurement procedure may consist of sampling, concentration and sample injection to the separation column. A flame photometric detector (FPD), a pulsed flame photometric detector (PFPD), an atomic emission detector (AED), a sulfur chemiluminescence detector (SCD), a mass spectrometer (MS), and the like, capable of selectively detecting trace amount of sulfur compounds with substantially linearity, may be used.
Electronic Device Cooling Cold Trap-Capillary GC
Sulfur compounds existing in the sample are concentrated at low temperature using a cold trap, desorbed at moderate temperature, and transferred into a syringe pump by the pressure of a carrier gas. The desorption occurs at moderate-to-low temperatures (e.g., about 100° C. or lower), not at high temperatures (e.g., above about ° C.). The concentrated sample transferred to the syringe pump is injected into the separation column and analyzed by the detector. The cold-trapped sample may also be thermally desorbed and injected into the separation column.
Detection of Trimethylamine
The concentration of trimethylamine in the air may be measured as follows. After sampling using an impinger and acidic filter paper, analysis may be performed by cold trap-packed column GC and headspace-capillary column GC.
Detection of Acetaldehyde, Propionaldehyde, Butyraldehyde, n-Valeraldehyde and Isovaleraldehyde
For simultaneous measurement of the concentration of acetaldehyde, propionaldehyde, butyraldehyde, n-valeraldehyde and isovaleraldehyde contributing to offensive odor, 2,4-dinitrophenylhydrazone (DNPH) derivatives of the aldehyde compounds may be formed and analyzed by HPLC and GC.
Dinitrophenylhydraziner (DNPH) Derivatization and HPLC/UV
DNPH derivatives formed by reacting carbonyl compounds with 2,4-dinitrophenylhydrazine (DNPH) are extracted with an acetonitrile solvent and analyzed by HPLC using a UV detector at 360 nm wavelength.
DNPH Derivatization and GC
DNPH derivatives formed by reacting carbonyl compounds with 2,4-dinitrophenylhydrazine (DNPH) may be extracted with an acetonitrile solvent and analyzed by GC after changing the solvent to ethyl acetate.
HPLC Instrument
The HPLC equipment for sample analysis may include a sample injection device, a pump, a separation column and a detector (UV detector). The separation column may be a reversed-phase column (ODS column) to which a nonpolar adsorbent is coated allowing control of the mobile phase solvent according to the mixing ratio. The sample loop of the injection device may be about 20-100 μL depending on the sample concentration.
GC Instruments
A capillary separation column may be used for GC, and a flame ionization detector (FID), a nitrogen phosphorus detector (NPD) or a mass spectrometer may be used as the detector.
Detection of Styrene
Styrene may be sampled at the site boundary. After sampling using a solid sorbent tube, a canister or a sample bag, analysis may be performed by cold trap-GC and solid-phase microextraction (SPME)-GC.
Detection of Toluene, Xylene, Methyl Ethyl Ketone, Methyl Isobutyl Ketone, Butyl Acetate, Styrene and Isobutyl Alcohol
The concentration of toluene, xylene, methyl ethyl ketone, methyl isobutyl ketone, butyl acetate, styrene and isobutyl alcohol, which are volatile compounds contributing to offensive odor, in the air may be simultaneously measured.
Toluene, xylene, methyl ethyl ketone, methyl isobutyl ketone, butyl acetate, styrene and isobutyl alcohol may be specified offensive odor substances. Sampling may be performed at the site boundary. The sample collected using a solid sorbent tube may be analyzed by GC after cold trapping and thermal desorption.
Detection of Propionic Acid, n-Butyric Acid, n-Valeric Acid and Isovaleric Acid
The concentration of the organic acids in the air may be measured as follows. After sampling using an alkaline-impregnated filter or by alkaline solution absorption, the collected sample may be pretreated by the headspace method to evaporate the organic acid components. Then, analysis may be performed by GC.
In another aspect, the present invention provides a method for preparing a fishy water odor composition from an air conditioner, including mixing two or more compounds selected from a group consisting of: ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.
In another aspect, the present invention provides a method for preparing a fishy water odor composition from an air conditioner, including mixing ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.
In an exemplary embodiment of the present invention, the method for preparing a fishy water odor composition from an air conditioner according to the present invention may include mixing: about 0.08-0.20 ppb of ammonia; about 10.00-12.00 ppb of acetaldehyde; about 40.00-45.00 ppb of toluene; about 14.00-18.00 ppb of m/p-xylene; about 8.00-12.00 ppb of o-xylene; about 1.00-4.00 ppb of methyl isobutyl ketone; about 8.00-13.00 ppb of butyl acetate; about 15.00-19.00 ppb of butanol; about 0.10-3.00 ppb of hexanal; about 0.10-3.00 ppb of decanol; about 8.00-13.00 ppb of 1,3,5-trimethylbenzene; about 0.10-3.00 ppb of acetone; about 38.00-42.00 ppb of dodecane; about 8.00-11.00 ppb of 2-butoxyethanol; about 0.10-4.00 ppb of 2-methoxyethanol; about 3.00-7.00 ppb of ethylbenzene; about 3.00-6.00 ppb of hexane; about 3.00-6.00 ppb of nonane; about 0.01-1.00 ppb of n-octane; and about 2.00-5.00 ppb of n-undecane.
EXAMPLES
The present invention will be described in more detail through examples. The following examples are for illustrative purposes only and it will be apparent to those skilled in the art not that the scope of this invention is not limited by the examples.
Example 1
Sensory Test
1) Selection of Vehicle Model
Odor was sampled from the air conditioner of a vehicle model A.
2) Sensory Test Method

    
    
        i) Three out of the four air conditioner exhausts were sealed hermetically.
        ii) For sensory test and gas sampling, the exhaust at the left side of the driver seat was sealed hermetically using a glass tube and a vinyl bag.
        iii) The air conditioner was operated at level 2 under internal ventilation condition.
        iv) The panelist was asked to smell the sample in the glass tube and evaluate the intensity and type of odor.
    
    


Table 1 describes the level of odor according to intensity which was used as the standard of sensory test and evaluation after preparation of the mixtures for reproducing the detected odor in Examples 3 and 4.










TABLE 1







Odor intensity
Level of odor









5
Irritating and intense odor



4
Strong odor



3
Weak but easily perceived odor



2
Perceived but slight odor



1
Almost unperceived odor



0
No odor










Example 2
Sampling Procedure
1) Selection of Vehicle Model
Sample was taken from the same vehicle as in Example 1.
2) Sensory Test Method

    
    
        i) Three out of the four air conditioner exhausts were sealed hermetically.
        ii) The exhaust at the left side of the driver seat was sealed hermetically using a glass tube and a vinyl bag.
        iii) The opening of a 10-L PE sample bag was connected to the glass tube.
        iv) The air conditioner was operated at level 2 under internal ventilation condition and gas sample was taken.
    
    


Example 3
Sample Analysis
The sample taken in Example 2 was analyzed by absorption spectrophotometry, headspace-gas chromatography with flame ionization detector (HS-GC/FID), gas chromatography with flame photometric detector (GC/FPD), high-pressure liquid chromatography with ultraviolet detector (HPLC/UV), gas chromatography/mass spectrometry (GC/MSD) and headspace-gas chromatography/mass spectrometry (HS-GC/MS).
Table 2 shows the result of detecting compounds contributing to offensive odor from the sample.











TABLE 2










Detection
Concentration (ppm)
















limit


Indoor


No.

Components
(ppm)
On
Off
air
















1
Representative
Toluene
330
39.362
42.751
29.539


2
components
m,p-Xylene
41
11.82
16.151
4.545


3

Methyl isobutyl ketone
17
2.062
2.866
0


4

Butyl acetate
16
8.454
11.842
1.578


5
Other VOCs
1,3-Cyclopentadiene,

1.157
1.409
0




2,3,4-tetramethyl






6

1-Butanol

9.005
18.168
6.038


7

1-Methoxy-2-propyl acetate

3.953
7.987
4.138


8

Benzene, 1,2,3-trimethyl- 

7.557
10.38
0


9

Benzene, 1,2,3-trimethyl- 

5.336
7.016
0


10

Benzene, 1,2,3-trimethyl-

7.891
10.37
5.754


11

Benzene, 1,3,5-trimethyl- 

7.364 
11.528
0


12

Benzene, 1-ethyl-2,3-dimethyl-

1.743 
2.154
0.775


13

Decane, 2,6,6-trimethyl-

84.088
98.805
0


14

Ethanol, 2-butoxy-

0
9.302
0


15

Ethylbenzene

4.747
5.779
0


16

Hexanal

1.634
1.878
1.151


17

Hexane

4.652 
4.976
1.953


18

Hexane, 3-methyl-

2.34
3.415
0


19

Hexanoic acid, methyl ester

0
1.358
0


20

Nonane

3.405
4.723
0
























TABLE 3













Primary standard
Standard gas for
Offensive odor







Density
gas
dilution
components























Det.

Injection
Bag


Bag


Bag
Injection





limit

vol.
vol.
Conc.
Conc.
vol.
Conc.
Conc.
vol.
vol.


No.

Components
(ppb)
M.W.
(μL)
(L)
(ppm)
(ppb)
(L)
(ppm)
(ppb)
(L)
(mL)
























1
Representative
Ammonia
150
17.03
0.70
1.0
3.0
76.727
25.6
3.0
1.0
0.1
6.0
0.04


2
components
Acetaldehyde
1.5
44.00
0.79
1.0
3.0
133.721
44.6
3.0
1.0
11.2
6.0
2.51


3

Toluene
330
92.14
0.87
1.0
3.0
70.096
23.4
3.0
1.0
42.8
6.0
18.30


4

m,p-Xylene
41
106.17
0.87
1.0
3.0
60.974
20.3
3.0
1.0
16.2
6.0
7.95


5
Other VOCs
o-Xylene
380
106.17
0.87
1.0
3.0
61.185
20.4
3.0
1.0
10.0
6.0
4.92


6

Methyl isobutyl ketone
17
100.16
0.80
1.0
3.0
59.712
19.9
3.0
1.0
2.9
6.0
1.44


7

Butyl acetate
16
116.16
0.88
1.0
3.0
56.694
18.9
3.0
1.0
11.8
6.0
6.27


8

Butanol

74.12
0.81
1.0
3.0
81.597
27.2
5.0
1.7
18.200
6.0
6.69


9

Hexanal

100.16
0.82
1.0
3.0 
61.129
20.4
6.0
2.0
1.900
6.0
0.93


10

Decanal

158.28
0.83
1.0 
3.0
39.154
13.1
24.0
8.0
1.900
6.0
1.46


11

1,3,5-Trimethylbenzene

120.20
0.86
2.0
6.0
53.671
17.9
27.0
9.0
11.528
6.0
6.44


12

Acetone

58.08
0.791
2.0
6.0
101.690
33.9
31.0 
10.3
1.579
6.0
0.47


13

Dodecane

170
0.75
2.0
6.0
32.853
11.0
37.0 
12.3
40.725
6.0
37.19


14

2-Butoxyethanol

118.18
0.90
2.0 
6.0
57.052
19.0
38.0
12.7
9.302
6.0
4.89


15

2-Methoxyethanol

76.1
0.97
2.0
6.0
94.879
31.6
39.0 
13.0 
2.861
6.0
0.90


16

Ethylbenzene

106.17
0.87
2.0
6.0
61.185
20.4
40.0
13.3
5.779
6.0
2.83


17

Hexane

86
0.66
2.0 
6.0
57.216
19.1
44.0
14.7 
4.976
6.0
2.61


18

Nonane

128.26
0.72
2.0
6.0
41.798
13.9
25.0
8.3
4.723
6.0
3.39


19

n-Octane

114.23
0.70
2.0
6.0
45.952
15.3
25.0
8.3
0.842
6.0
0.55


20

n-Undecane,

156.31
0.74
2.0
6.0
35.349
11.8
25.0
8.3
3.959
6.0
3.36




99%





















Example 4
Preparation of Fish Smell of Water Composition
A fishy water odor composition was prepared by mixing toluene, m-xylene, methyl isobutyl ketone, butyl acetate, hexanal, ethylbenzene, hexane and nonane according to the compositions described in Table 4.














TABLE 4







Detection



Bag
Injection




limit


Conc.
vol.
vol.



Components
(ppb)
M.W.
Density
(ppb)
(L)
(mL)






















Representative
Toluene
330
92.14
0.87
42.751
6
18.3


components
m-Xylene
41
106.17
0.87
16.151
6
7.95



Methyl isobutyl
17
100.16
0.8
2.866
6
1.44



ketone









Butyl acetate
16
116.16
0.88
11.842
6
6.27


Other VOCs
Hexanal

100.16
0.82
1.9
6
0.93



Ethylbenzene

106.17
0.87
5.779
6
2.83



Hexane

86
0.66
4.976
6
2.61



Nonane

128.26
0.72
4.723
6
3.39









The odor of the samples obtained from the air conditioner of the vehicle model A was fishy water odor of intensity 3-4, as described in Table 5.
The odor of the fishy water odor composition prepared from a combination of the aforementioned components was also fishy water odor of intensity 3-4, similar to that from the air conditioner.









TABLE 5







Odor from vehicle air
Characteristic
Dust odor/fishy water


conditioner

odor/floorcloth odor


Fishy water odor
Characteristic
Grass odor/fishy water


composition prepared from

odor/liquid soap


detected compounds

odor/fish-like odor


Note
Reproducibility
Fishy water odor









The features and advantages of the present disclosure may be summarized as follows.

    
    
        (i) Through the analysis method of the present invention, the compounds contributing to the fishy water odor from an air conditioner may be identified and quantified.
        (ii) The fishy water odor may be reproduced from a combination of the compounds identified by the analysis method of the present invention.
        (iii) The reproduced fishy water odor may provide significant data required for development of an apparatus and a method for removing specific odor.
    
    


The present invention has been described in detail with reference to exemplary embodiments thereof. However, it will be appreciated by those skilled in the art that various changes and modifications may be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the accompanying claims and their equivalents.

## Claims

1. A detected fishy water odor composition from an air conditioner comprising ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.

2. The detected fishy water odor composition from an air conditioner according to claim 1, comprises:
0.08-0.20 ppb of ammonia;
10.00-12.00 ppb of acetaldehyde;
40.00-45.00 ppb of toluene;
14.00-18.00 ppb of m/p-xylene;
8.00-12.00 ppb of o-xylene;
1.00-4.00 ppb of methyl isobutyl ketone;
8.00-13.00 ppb of butyl acetate;
15.00-19.00 ppb of butanol;
0.10-3.00 ppb of hexanal;
0.10-3.00 ppb of decanol;
8.00-13.00 ppb of 1,3,5-trimethylbenzene;
0.10-3.00 ppb of acetone;
38.00-42.00 ppb of dodecane;
8.00-11.00 ppb of 2-butoxyethanol;
0.10-4.00 ppb of 2-methoxyethanol;
3.00-7.00 ppb of ethylbenzene;
3.00-6.00 ppb of hexane;
3.00-6.00 ppb of nonane;
0.01-1.00 ppb of n-octane; and
2.00-5.00 ppb of n-undecane.

3. A method for preparing a fishy water odor composition from an air conditioner, comprising mixing ammonia, acetaldehyde, toluene, m/p-xylene, o-xylene, methyl isobutyl ketone, butyl acetate, butanol, hexanal, decanol, 1,3,5-trimethylbenzene, acetone, dodecane, 2-butoxyethanol, 2-methoxyethanol, ethylbenzene, hexane, nonane, n-octane and n-undecane.

4. The method according to claim 3, further comprises mixing:
0.08-0.20 ppb of ammonia;
10.00-12.00 ppb of acetaldehyde;
40.00-45.00 ppb of toluene;
14.00-18.00 ppb of m/p-xylene;
8.00-12.00 ppb of o-xylene;
1.00-4.00 ppb of methyl isobutyl ketone;
8.00-13.00 ppb of butyl acetate;
15.00-19.00 ppb of butanol;
0.10-3.00 ppb of hexanal;
0.10-3.00 ppb of decanol;
8.00-13.00 ppb of 1,3,5-trimethylbenzene;
0.10-3.00 ppb of acetone;
38.00-42.00 ppb of dodecane;
8.00-11.00 ppb of 2-butoxyethanol;
0.10-4.00 ppb of 2-methoxyethanol;
3.00-7.00 ppb of ethylbenzene;
3.00-6.00 ppb of hexane;
3.00-6.00 ppb of nonane;
0.01-1.00 ppb of n-octane; and
2.00-5.00 ppb of n-undecane.