BIOPESTICIDE REGISTRATION ACTION DOCUMENT

Bacillus thuringiensis Cry1A.105 and Cry2Ab2 Insecticidal Proteins and the Genetic Material 

Necessary for Their Production in Corn 

[PC Codes 006515 (Cry2Ab2), 006514 (Cry1A.105)] 

U.S. Environmental Protection Agency 

 Office of Pesticide Programs 

 Biopesticides and Pollution Prevention Division
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

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TABLE of CONTENTS 

I. OVERVIEW............................................................................................................................................................

A. EXECUTIVE SUMMARY ....................................................................................................................................

B. USE PROFILE........................................................................................................................................................

C. REGULATORY HISTORY ..................................................................................................................................

II. SCIENCE ASSESSMENT.......................................................................................................................................

A. PRODUCT CHARACTERIZATION .................................................................................................................

B. HUMAN HEALTH ASSESSMENT OF CRY1A.105 .........................................................................................

C. HUMAN HEALTH ASSESSMENT CRY2AB2 ..................................................................................................

D. ENVIRONMENTAL ASSESSMENT FOR MON 89034.....................................................................................

E. INSECT RESISTANCE MANAGEMENT (IRM).............................................................................................

F. BENEFITS AND PUBLIC INTEREST FINDING.............................................................................................

III. TERMS AND CONDITIONS OF REGISTRATION.........................................................................................

A. TERMS AND CONDITIONS FOR MON 89034 AS AMENDED ON DECEMBER 15, 2008:.........................................

B. DATA REQUIRED TO SUPPORT MON 89034 X MON 88017 AS AMENDED .........................................................

ON DECEMBER 15, 2008:...........................................................................................................................................

IV. REGULATORY POSITION FOR CRY1A.105, AND CRY2AB2 ....................................................................

V. BIBLIOGRAPHY: .................................................................................................................................................
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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BIOPESTICIDE REGISTRATION ACTION TEAM

Office of Pesticide Programs:

Biopesticides and Pollution Prevention Division

Product Characterization and Human Health 

John Kough, Ph.D. 

Rebecca Edelstein, Ph.D. 

Environmental Fate and Effects 

Zigfridas Vaituzis, Ph.D. 

Tessa Milofsky, M.S. 

Mika Hunter 

Insect Resistance Management 

Sharlene Matten, Ph.D. 

Alan Reynolds, M.S. 

Jeannette Martinez 

Benefits Assessment 

Jeannine Kausch 

Registration Support 

Mike Mendelsohn 

Susanne Cerrelli 

Jeannine Kausch 

Office of General Council 

Chris Kaczmarek, Esq. 

Keith Matthews, Esq. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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I. OVERVIEW 

 A. EXECUTIVE SUMMARY 

On June 10, 2008, EPA conditionally registered a plant-incorporated protectant product, event 

MON 89034 corn, containing two new active ingredients, Bacillus thuringiensis Cry1A.105 and 

Cry2Ab2 insecticidal proteins, and the genetic material necessary for their production. EPA also 

conditionally registered another product, MON 89034 x MON 88017 corn, which contains a 

previously registered Bacillus thuringiensis Cry3Bb1 protein in addition to the two new active 

ingredients. These registrations will expire on September 30, 2010. The Agency determined 

that the use of these pesticide products is in the public interest and that their use will not cause 

any unreasonable adverse effects on the environment during the time the products are registered. 

The registrant for both products is Monsanto Company (“Monsanto”). 

 Event MON 89034 corn produces its own insecticide derived from Bacillus thuringiensis (Bt), a 

naturally occurring soil bacterium. The Bt proteins produced in this product, called Cry1A.105, 

and Cry2Ab2, have been shown to effectively control highly destructive lepidopteran corn pests, 

including European corn borer (ECB), corn earworm (CEW), southwestern corn borer (SWCB), 

fall armyworm (FAW), and sugarcane borer (SCB), in field trials conducted during the 2003-

2004 growing seasons in Puerto Rico and the United States. These pests feed on the base of 

seedlings and on the stalk, leaf, and ear tissue of corn plants, thereby destroying the entire plant, 

weakening the stalk, and/or damaging the ear. In areas where one or more of these pests is 

prevalent (e.g., the corn belt), significant financial losses are realized from decreased corn yields 

and increased expenditures on chemical pest control agents, including organophosphate, 

carbamate and pyrethroid insecticides. 

On June 10, 2008, when the conditional, time-limited registrations of MON 89034, and MON 

89034 x MON 88017 were issued, the non-Bt corn borer refuge was required to be at least 20% 

for the corn belt. On December 15, 2008, EPA amended these product registrations to allow a 

reduction in the structured corn borer refuge requirement (5%) in the non-cotton-growing regions 

of the corn belt. 

The data required to satisfy the conditions of these registrations are listed in Section IV, 

“Regulatory Position for Cry1A.105, and Cry2Ab2.” 

Product Characterization

MON 89034 was developed by Agrobacterium-mediated transformation of corn using the 2TDNA plasmid vector PV-ZMIR245. The transformation produces two Bacillus thuringiensis

proteins, Cry1A.105 and Cry2Ab2. Cry1A.105 is a chimeric protein composed of portions of 

Cry1Ab, Cry1Ac, and Cry1F proteins. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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Mammalian Toxicity and Allergenicity Assessment

When proteins are toxic, they are known to act via acute mechanisms and at very low dose levels 

(Sjoblad, Roy D., et al., “Toxicological Considerations for Protein Components of Biological 

Pesticide Products,” Regulatory Toxicology and Pharmacology 15, 3-9 (1992)). The acute oral 

toxicity data submitted by Monsanto demonstrated the lack of mammalian toxicity in rats and 

mice exposed to pure Cry1A.105 and Cry2Ab2 protein at doses well above the maximum levels 

anticipated in treated crops, based upon the demonstrated expression values of these two 

proteins. 

 

Since no acute effects were observed in the submitted studies, even at relatively high dose levels, 

the Cry1A.105 and Cry2Ab2 proteins are not considered to be toxic. This conclusion was 

supported by amino acid sequence comparisons of the Cry1A.105 and Cry2Ab2 proteins with 

databases of known toxic proteins, which showed no similarities that would raise a safety 

concern. In addition, the data submitted by Monsanto demonstrated that the Cry1A.105 and 

Cry2Ab2 proteins were substantially degraded by heat when examined by immunoassay. This 

instability to heat would decrease the potential for dietary exposure to intact Cry1A.105 and 

Cry2Ab2 proteins in cooked or processed foods. These biochemical features, along with the lack 

of adverse results in the acute oral toxicity tests, support the Agency’s conclusion that there is a 

reasonable certainty of no harm from dietary exposure to Cry1A.105 and Cry2Ab2 containing 

crops. 

Since Cry1A.105 and Cry2Ab2 are proteins, their potential for food allergenicity was also 

considered. Currently, no definitive tests for determining the allergenic potential of novel 

proteins exist. Therefore, EPA uses a “weight-of-evidence” approach when considering the 

allergenic potential for a PIP protein, and bases its conclusions upon the following factors: the 

source of the trait, the amino acid sequence compared with known allergens, and the biochemical 

properties of the protein, including in vitro digestibility in simulated gastric fluid (SGF) and 

glycosylation. This is consistent with the approach outlined in the Annex to the Codex 

Alimentarius “Guideline for the Conduct of Food Safety Assessment of Foods Derived from 

Recombinant-DNA Plants.” The Agency’s allergenicity assessment for the Cry1A.105 and 

Cry2Ab2 proteins follows: 

1. Source of the traits. Bacillus thuringiensis is not considered to be a source of allergenic 

proteins. 

2. Amino acid sequence. A comparison of the amino acid sequences of Cry1A.105 and 

Cry2Ab2 with known allergens showed no sequence similarity or identity at the level of 

eight contiguous amino acid residues, which is considered to be the smallest amino acid 

sequence necessary to induce an immune response. 

3. Digestibility. The Cry1A.105 and Cry2Ab2 proteins were digested rapidly in simulated 

gastric fluid containing pepsin, the enzyme produced by the stomach that digests proteins 

so they can be absorbed as nutrients into the body via the small intestine. The rapid 

degradation of Cry1A.105 and Cry2Ab2 in the simulated gastric environment indicated 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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that the intact protein will not pass from the stomach into the intestinal lumen, where 

sensitization of the immune system to food allergens occurs. 

4. Glycosylation. Cry1A.105 and Cry2Ab2 proteins expressed in corn are not 

glycosylated.1

5. Conclusion. EPA concluded that the potential for Cry1A.105 and Cry2Ab2 to be a food 

allergen is minimal. 

The information on the safety of pure Cry1A.105 and Cry2Ab2 proteins provides adequate 

justification to address possible exposures in all corn crops. 

Environmental Hazard Assessment

Maximum hazard dose toxicity testing on representative beneficial organisms from several taxa 

was performed in support of the registrations of Cry1A.105 and Cry2Ab2 proteins expressed in 

corn. The toxicity of the Cry1A.105 and Cry2Ab2 proteins was evaluated on several species of 

invertebrates, including the lady beetle, minute pirate bug, parasitic hymenoptera, Collembola, 

Daphnia, honey bee, and earthworm. Developmental observations were also made in the lady 

beetle, minute pirate bug, and honeybee studies. Observations of possible reproductive effects 

were also made in the Collembolan studies. In addition, earthworm studies were voluntarily 

submitted to the Agency to ascertain the potential effects of the Cry1A.105 and Cry2Ab2 

proteins on beneficial decomposer species. Avian dietary studies and soil fate data were also 

submitted. 

The test substances used for the studies submitted in support of the MON 89034 registrations 

included bacterially produced, purified Cry1A.105 and Cry2Ab2 proteins, and MON 89034 corn 

leaf tissue, pollen, and grain. The October 2000 FIFRA Science Advisory Panel (SAP) 

recommended that while actual plant material is the preferred test material, bacterially derived 

protein is also a valid test substance, particularly in scenarios where test animals do not normally 

consume corn plant tissue and where large amounts of Cry protein (Cry protein concentrations 

that exceed levels present in plant tissue) are needed for maximum hazard dose testing. An 

insect feeding study, which compared the relative potency of plant produced Cry1A.105 and 

Cry2Ab2 proteins to the microbe produced proteins, indicated that plant produced protein was 

similar in toxicity to the bacterially produced protein (Edelstein Memo, November 7, 2007). 

The potential interactions between the Cry1A.105 and Cry2Ab2 proteins was addressed in a 

memorandum for the MON 89034 Experimental Use Permit accompanying the Agency’s 

review, “Evaluation of the Potential for Interactions Between the Bacillus thuringiensis Proteins 

Cry1A.105 and Cry2Ab2,” (Hunter, M., July 6, 2006). The study provided evidence that the 

proteins do not interact in either an antagonistic or synergistic manner, and that there will not be 

 1

 Although this was only demonstrated in corn, these expressed proteins are unlikely to be glycosylated if produced in 

any other crops since the mechanisms of protein glycosylation are similar in different plants (Lerouge, P. Cabanes-Macheteau, 

M., Rayon, C., Fichette-Lainé, A-C., Gomord, V., and Faye, L., “N-Glycoprotein biosynthesis in plants: recent developments and 

future trends,” Plant Molecular Biology 38: 31-48, 1998 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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any unexpected interactions with regard to target and non-target insects. New data on the 

potential interaction between the combined Cry1A.105, Cry2Ab2 and Cry3Bb1 proteins were 

submitted. The results from that study demonstrated that the combined Cry1A.105 and 

Cry2Ab2 activity was not affected by the Cry3Bb1 protein, and that the Cry3Bb1 activity was 

unaffected by combined Cry1A.105 and Cry2Ab2 activity (MRIDs 469513-05 & 469513-06). 

Insect Resistance Management

Monsanto has demonstrated that the Cry1A.105 and Cry2Ab2 toxins have different modes of 

action, and consequently, a low likelihood of cross-resistance. Therefore, Cry1A.105 and 

Cry2Ab2 are suitable partners in a pyramided product. Monsanto has also shown that there is a 

low likelihood of cross-resistance between Cry1A.105 and Cry1Ab. Monsanto has previously 

demonstrated that there is a low likelihood of cross-resistance between Cry2Ab2 and Cry1Ac. 

Both Cry1Ab and Cry1Ac are expressed in other registered Bt corn and Bt cotton PIPs. 

Monsanto did not, however, address the likelihood of cross-resistance between Cry1A.105 and 

Cry1Ac, and Cry1Fa (Bt proteins already in existing Bt corn and Bt cotton products), and what 

impact such cross-resistance would have on the durability of MON 89034. As a result, 

Monsanto was required to provide additional information on cross-resistance of Cry1A.105 and 

Cry1Fa and Cry1Ac (including binding site models and use of resistant colonies) for the target 

pests and determine how such cross-resistance could impact the durability of MON 89034. 

Monsanto originally proposed that a 5% structured refuge, rather than the 20% structured refuge 

required for other Bt corn registrations, be applied to field corn uses of MON 89034 in the U.S. 

Corn Belt. But, the data and simulation modeling in Monsanto’s initial application did not 

support the 5% proposed refuge for MON 89034 in the Corn Belt. There were uncertainties 

regarding the dose determination for susceptible and heterozygote (i.e., partially resistant) insects 

(ECB, SWCB, CEW, and FAW), the cross-resistance potential of Cry1A.105, Cry1Ac and 

Cry1Fa and any impacts on the durability of MON 89034, and limitations in the simulation 

modeling. Therefore, the field corn uses of MON 89034 in the Corn Belt were registered with a 

20% refuge requirement until such time as Monsanto could address the uncertainties. EPA 

determined, however, that the data did support reduction of the refuge from 50% to 20% in 

cotton-growing regions in the southeastern U.S., where a 50% non-Bt corn refuge has been 

required for other Bt corn registrations.

Subsequent to the registrations of the event MON 89034 corn and MON 89034 x MON 88017 

corn products, Monsanto submitted additional data and an analysis of potential resistance risks to 

support an amendment to reduce the required non-Bt corn refuge for MON 89034 corn from 20% 

to 5% in the U.S. Corn Belt. After reviewing these data, EPA determined that a 5% refuge in the 

U.S. Corn Belt should not significantly increase the risk of resistance for ECB, CEW, and 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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SWCB. Monsanto sufficiently addressed the requirement to analyze potential cross resistance in 

existing Bt corn and Bt cotton products for Cry1A.105 and Cry1Fa, but additional analysis and 

information is still needed to fully assess the cross resistance potential for Cry1Ac and 

Cry1A.105. 

B. USE PROFILE

Active Ingredient Name: Bacillus thuringiensis Cry1A.105, and Cry2Ab2 insecticidal 

protein and the genetic material necessary for their 

production in corn

Trade and Other Name(s): MON 89034 

OPP Chemical Codes: 006515 (Cry2Ab2) and 006514 (Cry1A.105) 

Basic Manufacturer: Monsanto Company 

800 North Lindbergh Blvd. 

St. Louis, MO 63167 

Type of Pesticide: Plant-incorporated Protectant 

 

Uses: Field Corn and Sweet Corn 

Target Pests for Active Ingredient: European corn borer (Ostrinia nubilalis), 

Southwestern corn borer (Diatraea grandiosella), Southern cornstalk borer (Diatraea 

crambidoides), Corn earworm (Helicoverpa zea), Fall armyworm (Spodoptera 

frugiperda), Corn stalk borer (Papaipema nebris), and Sugarcane borer (Diatreae 

saccharalis)

C. REGULATORY HISTORY 

Monsanto previously submitted an Experimental Use Permit (EUP) application for events MON 

89034, MON 88017, and MON 89034 x MON 88017. MON 89034 was developed by 

Agrobacterium-mediated transformation of corn using the 2T-DNA plasmid vector PVZMIR245, and produces two Bacillus thuringiensis (Bt) proteins, Cry1A.105 and Cry2Ab2. 

These proteins are intended to provide protection from feeding damage caused by a number of 

lepidopteran pests. Cry1A.105 is a chimeric protein composed of portions of Cry1Ab, Cry1Ac, 

and Cry1F proteins. On July 17, 2006, EPA established temporary exemptions from the 

requirement of a tolerance for both Cry1A.105 (71 FR 40427 and 72 FR 20434; 40 CFR 

174.502) and Cry2Ab2 (71 FR 40431 and 72 FR 20434; 40 CFR 174.503) in the food and feed 

commodities of corn; these exemptions were set to expire on June 30, 2009. 
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On November 15, 2006, Monsanto submitted petitions to EPA under the Federal Food, Drug, 

and Cosmetic Act (FFDCA), as amended by the Food Quality Protection Act of 1996 (FQPA), 

requesting amendment of the existing temporary tolerances in 40 CFR 174.503 for the Bacillus 

thuringiensis Cry2Ab2 insecticidal protein to establish a permanent exemption from the 

requirement of a tolerance for the Bacillus thuringiensis Cry2Ab2 insecticidal protein and in 40 

CFR 174.502 for the Bacillus thuringiensis Cry 1A.105 insecticidal protein to establish a 

permanent exemption from the requirement of a tolerance for the Bacillus thuringiensis Cry 

1A.105 insecticidal protein in field corn, sweet corn, and popcorn. 

On September 29, 2006, Monsanto submitted an application to register MON 89034 and MON 

89034 x MON 88017 under Section 3 of the Federal Insecticide, Fungicide and Rodenticide Act 

(FIFRA). 

On March 9, 2007, Monsanto resubmitted petitions to EPA under the Federal Food, Drug, and 

Cosmetic Act (FFDCA), requesting amendment of the existing temporary tolerances in 40 CFR 

174.503 for the Bt Cry2Ab2 insecticidal protein to establish a permanent exemption from the 

requirement of a tolerance for the Bt Cry2Ab2 insecticidal protein, and in 40 CFR 174.502 for 

the Bt Cry 1A.105 insecticidal protein to establish a permanent exemption from the requirement 

of a tolerance for the Bt Cry 1A.105 insecticidal protein in all crops and agricultural commodities 

On June 10, 2008, conditional registrations were issued for MON 89034 and MON 89034 x 

MON 88017 products. 

On July 2, 2008 (73 FR No. 128), the existing permanent exemption from the requirement of a 

tolerance for residues of the Bacillus thuringiensis Cry2Ab2 protein under 174.519 was amended 

to include corn or cotton when used as a plant-incorporated protectant in the food and feed 

commodities: field corn, sweet corn, popcorn, cotton seed, cotton oil, cotton meal, cotton hay, 

cotton hulls, cotton forage, and cotton gin byproducts in accordance with good agricultural 

practices. 

On July 16, 2008 (73 FR No. 137), the Agency established permanent exemptions from the 

requirement of a tolerance for residues of the Bacillus thuringiensis Cry1A.105 protein in or on 

the food and feed commodities: field corn, sweet corn, and popcorn when used as plant 

incorporated protectant in all food commodities in accordance with good agricultural practices. 

On December 15, 2008, the conditional registrations were amended for MON 89034 and MON 

89034 x MON 88017, to allow for a 5% structured refuge in the corn belt (in non-cotton growing 

regions) for corn borers. 
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II. SCIENCE ASSESSMENT

 

A. PRODUCT CHARACTERIZATION 

MON 89034 was developed by Agrobacterium-mediated transformation of corn using the 2TDNA plasmid vector PV-ZMIR245 and produces two Bacillus thuringiensis proteins, Cry1A.105 

and Cry2Ab2. These proteins are intended to provide protection from feeding damage caused by 

a number of lepidopteran pests. Cry1A.105 is a chimeric protein composed of portions of 

Cry1Ab, Cry1Ac, and Cry1F proteins. 

Transformation System: 

PV-ZMIR245 is a binary vector containing two separate transfer DNAs (2T-DNA). The first TDNA contains the cry1A.105 and the cry2Ab2 expression cassettes. The second T-DNA contains 

the nptII (neomycin phosphotransferase II) expression cassette. The cry1A.105 expression 

cassette contains the cry1A.105 coding sequence under the regulation of the e35S promoter, 

Ract1 intron, and the Hsp17 3’ end sequence. The cry2Ab2 expression cassette contains the 

cry2Ab2 coding sequence under the regulation of the FMV promoter, the Hsp70 intron, a 

chloroplast transit peptide (TS-SSU-CTP), and the nos 3’ end sequence. The nptII expression 

cassette contains the nptII coding sequence under the regulation of the CaMV 35S promoter and 

the nos 3’ end sequence. During transformation, both T-DNAs were inserted into the genome. 

The nptII selectable marker gene was used to select for transformed cells. Traditional breeding 

was then used to isolate plants that only contain the cry1A.105 and cry2Ab2 expression cassettes 

and not the nptII expression cassette. 

Characterization of the DNA Inserted in the Plant and Inheritance and Stability: 

Characterization of the DNA isolated from event MON 89034 corn using restriction enzyme 

digests and Southern blot analysis as well as DNA sequencing indicates that the DNA was 

inserted in the corn genome at a single locus, and the insert contains one copy each of the 

cry1A.105 and cry2Ab2 expression cassettes. There were no other detectable elements other than 

those associated with the respective cassettes. No backbone sequences from plasmid PVZMIR245 or nptII coding sequences were detected in the corn genome. Southern blot analysis 

also demonstrated the stability of the insert over multiple generations. DNA sequencing 

indicated that the genetic elements were present in the inserted DNA as expected except that the 

e35S promoter was modified, and the right border sequence present in PV-ZMIR245 was 

replaced by a left border sequence in MON 89034. 

Protein Characterization: 

Protein characterization data demonstrate that the plant-produced Cry1A.105 and Cry2Ab2 

proteins have biochemical and functional activities that are similar to those of the E. coliproduced proteins that were used in several toxicity studies. The following techniques were used 

to characterize and compare the plant-produced and the E. coli-produced proteins: sodium 

dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), western blot analysis, 

densitometry, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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spectrometry, glycosylation analysis, N-terminal amino acid sequencing, and insecticidal activity 

assays. Glycoslyation analysis indicated that the proteins are not glycoslyated. These analyses 

demonstrated the structural and functional similarity between the plant-produced and the E. coliproduced Cry1A.105 and Cry2Ab2 proteins and justified the use of E. coli-produced proteins in 

toxicity studies. Monsanto also provided information showing the similarity between 

Cry1A.105, Cry1Ab, and Cry1Ac. 

In addition, Monsanto provided information comparing the expected (deduced) amino acid 

sequence of Cry2Ab2 expressed in MON 89034 corn, the native form of the protein in B. 

thuringiensis, and in Bollgard II cotton. Monsanto stated that Cry2Ab2 in MON 89034 and 

Bollgard II are identical. Different chloroplast transit peptide sequences were used in the 

different products; however, these are expected to be cleaved and degraded in the plants upon 

uptake into the chloroplasts. When Monsanto attempted to determine the N-terminal sequence of 

Cry2Ab2 from MON 89034 or Bollgard II, the results indicated that the N-terminus is blocked in 

both. Therefore, Monsanto was unable to determine the cleavage site of the chloroplast transit 

peptide. Because the chloroplast transit peptides used in MON 89034 and Bollgard II have 

potential cleavage sites (methionine) three amino acids upstream from the start of the Cry2Ab2 

protein sequence, the Cry2Ab2 produced in Bollgard II and in MON 89034 may differ by one 

amino acid (leucine vs. glutamine) if the cleavage site is within the transit peptide. The E. coliproduced Cry2Ab2 protein used in the toxicity studies for MON 89034 includes the three 

additional amino acids from the chloroplast transit peptide at the N-terminus. Monsanto stated 

that the Cry2Ab2 proteins produced in MON 89034 and Bollgard II are variants of the wild type 

Cry2Ab2 protein produced in B. thuringiensis. The Bt-produced protein was used in some of the 

previously submitted studies that are cited to support the ecological risk assessment for MON 

89034. Monsanto therefore submitted a study demonstrating that the E. coli-produced Cry2Ab2 

and the Bt-produced Cry2Ab2 have equivalent biological activity (EC50 values and rates of 

growth inhibition) in a larval corn earworm diet-incorporation bioassay. 

Analytical Detection Methods: 

Short descriptions of enzyme-linked immunosorbent assay (ELISA) methods for detecting and 

quantifying Cry1A.105 and Cry2Ab2 as well as standard operating procedures for the methods 

were provided with the registration application. Monsanto stated that these methods have been 

validated and provided validation results in an appendix to MRID 46951403; but an independent 

lab validation study was not provided for either method. In addition, Monsanto did not indicate 

whether the ELISA method for Cry1A.105 will distinguish between Cry1A.105, Cry1Ab, 

Cry1Ac, and Cry1F. Since Cry1A.105 contains portions of all three proteins, there may be 

cross-reactivity in the assay. Monsanto also provided a study demonstrating that a commercially 

available qualitative immunochromatographic test strip can detect Cry2Ab2 in MON 89034 corn. 

Since event MON 89034 is the only product that expresses Cry1A.105 and the only corn product 

that expresses Cry2Ab2, the detection method for Cry2Ab2 can be used for detecting both 

Cry2Ab2 and Cry1A.105. The presence of Cry2Ab2 in corn should also indicate the presence of 

Cry1A.105. Monsanto has provided an independent lab validation of this method (MRID 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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47731601) as required in the conditions of registration. An evaluation of the independent lab 

validation method is currently pending. 

Protein Expression: 

Expression level data were provided for Cry1A.105 and Cry2Ab2 in different plant tissues and at 

different growth stages. Both proteins are expressed at relatively low levels in event MON 

89034 corn. The data were produced using ELISA methods for each protein. Summary results 

are provided below in Table 1. Table 2 provides summaries of the product characterization 

studies and data provided. 

Table 1. Mean Expression Levels of Cry1A.105 and Cry2Ab2 from MON 89034 Plant 

Tissues 

Tissue Type Cry1A.105 

(μg/g dry weight + standard 

deviation)* 

Cry2Ab2 

(μg/g dry weight + standard 

deviation)* 

Leaf 72 + 14 – 520 + 130 130 + 34 – 180 + 59 

Root 11 + 1.4 -79 + 17 21 + 5.9 -58 + 18 

Whole Plant 100 + 26 – 380 + 90 39 + 16 – 130 + 51 

Pollen 12 + 1.7 0.64 + 0.091 

Silk 26 + 3.9 71 + 35 

Forage 42 + 9.4 38 + 14 

Grain 5.9 + 0.77 1.3 + 0.36 

*Ranges reflect means at different growth stages for the first three tissue types 
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Table 2. Product Characterization Data Submitted 

Study Type/Title Summary MRID #

Characterization of the 

inserted DNA/ Summary 

of Southern Blot 

Analyses of MON 

89034 and MON 89697 

Corn2

Southern blot analyses indicate that MON 89034 and MON 89597 have the 

introduced DNA inserted in the corn genome at a single locus and contain 

one copy each of the cry1A.105 and cry1A.105 expression cassettes. All 

expression elements are shown to be present in each of the inserts, and there 

are no other elements detectable other than those associated with the 

respective cassettes. No backbone sequences from plasmid PV-ZMIR245 or 

nptII coding sequences were detected in the corn genome. 

Classification: ACCEPTABLE

46694501 

Analytical detection 

method/Qualitative 

Detection Method for 

the Cry2Ab2 Protein in 

Corn Leaf and Seed of 

MON 89034 and MON 

895972 

A commercially available qualitative immunochromatographic test strip 

(QuickStixTM kit AS 005 LS) was obtained from EnviroLogix Inc. to 

determine if the strips can detect the Cry2Ab2 protein produced in MON 

89034 and MON 89597. The QuickStixTM kit AS 005 LS detected the 

presence of Cry2Ab2 in MON 89034 and 89597. It was demonstrated that 

extracts of leaves or seed from MON 89034 or MON 89597 (both 

expressing Cry2Ab2) can be distinguished from corn plants that do not 

express the Cry2Ab2 protein.

Classification: ACCEPTABLE

46694503 

Characterization of the 

active ingredient/

Structural and 

Functional Similarity of 

the Cry1A.105 Protein 

to Cry1A Class of 

Bacillus thuringiensis

Proteins: Final Report2

A summary of current information about the structural and functional 

similarities of the Cry1A.105 protein to other Bt Cry1 proteins is presented 

in this submission. The Cry1A.105 protein is chimeric, with overall amino 

acid sequence identity to the Cry1Ac, Cry1Ab and Cry1F proteins of 93.6, 

90.0 and 76.7%, respectively. A structural model of the Cry1A.105 protein 

was developed using the X-ray crystal structure of the Cry1Aa protein. The 

model demonstrated high overall main chain structural similarity with 

Cry1Aa. Models of Cry1Ab and Cry1Ac were also prepared using the 

Cry1A.105 model. Comparison of the aligned folds of all three proteins 

showed that Cry1Ab and Cry1A.105 have essentially the same main chain 

structure, and that Cry1Ac differs slightly in its main chain structure from 

the other two in domain III. Thus, comparison of the modeled crystal 

structures of the Cry1A.105, Cry1Ab, and Cry1Ac with that of the 

experimental Cry1Aa X-ray crystal structure demonstrated high structure 

similarity between the four proteins. 

Monsanto also summarized results from bioactivity assays using 

Cry1A.105, Cry1Ab, and Cry1Ac in this submission and indicates that 

complete study reports will be submitted for registration. Monsanto states 

that purified E.coli-produced Cry1A.105 protein had significant activity 

against each representative lepidopteran insect larvae in laboratory diet 

bioassays. Tests species included; black cutworm (Agrotis ipsilon), corn 

earworm (Helicoverpa zea), fall armyworm (Spodoptera frugiperda) and 

European corn borer (Ostrinia nubilalis). Cry1A.105 insecticidal activity 

was similar to other Cry1 proteins (i.e., Cry1Ac, Cry1F, Cry1Ab). 

Coleopteran and heteropteran larvae showed no indication of sensitivity to 

46694601 

 2

 Study submitted with EUP request and reviewed in memorandum from R. Edelstein and I. Barsoum to M. 

Mendelsohn dated June 16, 2006. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

14

Study Type/Title Summary MRID #

the Cry1A.105 protein. The results of tests with purified Cry1A.105 

protein against non-target invertebrates from different orders, such as honey 

bee, minute pirate bug, earthworms, parasitic hymenoptera and ladybird 

beetle, demonstrated no meaningful activity. Corn tissues from MON 

89034 were tested in a bioassay for potential activity of the Cry1A.105 and 

Cry2Ab2 proteins against Collembola (Folsomia candida), Daphnia magna

and bobwhite quail with results indicating no effect on the tested non-target 

organisms. 

Classification: ACCEPTABLE 

Characterization of the 

active 

ingredient/Characterization of the Cry1A.105 

Protein Purified from the 

Corn Grain of MON 

89034 and Comparison 

of the Physiochemical 

and Functional 

Properties of the PlantProduced and E. coliProduced Cry1A.105 

Proteins2

The physicochemical properties and functional properties of the plantproduced Cry1A.105 were analyzed and compared with the properties of the 

E. coli produced Cry1A.105 using sodium dodecyl sulfate polyacrylamide 

gel electrophoresis (SDS-PAGE), western blot analysis, densitometry, 

matrix assisted laser desorption/ionization time of flight (MALDI-TOF) 

mass spectrometry, glycosylation analysis, and a Cry1A.105 insecticidal 

activity assay. Similar immunoreactive bands migrating between 

approximately 85 and 130 kDa were observed in the plant-produced 

Cry1A.105 and E. coli-produced reference samples, and the full-length 

Cry1A.105 protein (~130 kDa) was observed in both the plant-produced and 

E. coli-produced protein samples. MALDI-TOF mass spectrometry analysis 

of the ~130 kDa band after trypsin digestion yielded peptide masses 

consistent with peptide masses of the predicted sequence of the Cry1A.105 

protein. The identified peptide masses yielded 43.8% overall coverage of 

the expected peptide sequence (516 of the 1177 amino acids). 

Immunoreactivity with the N-terminal peptide antibody demonstrated that 

the N-terminus in the plant-produced full-length Cry1A.105 protein was 

intact. Glycosylation analysis demonstrated that neither the plant-produced 

nor the E. coli-produced Cry1A.105 protein is glycosylated. The plantproduced and E. coli-produced proteins gave similar results in the corn 

earworm diet-incorporation bioactivity assay: the mean EC50 values for the 

plant-produced Cry1A.105 protein and E. coli-produced reference standard 

were determined to be 0.0074 and 0.012 μg Cry1A.105 per mL diet, 

respectively. The results of this study demonstrate the structural and 

functional similarity between the plant-produced and the E. coli-produced 

Cry1A.105 proteins. 

Classification: ACCEPTABLE 

46694604 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

15

Study Type/Title Summary MRID #

Characterization of the 

inserted DNA/ Amended 

Report for MSL-20072: 

Molecular Analysis of 

Corn MON 89034 

The DNA inserted in event MON 89034 was characterized by Southern blot 

analysis and DNA sequencing. Southern blot analysis indicates that MON 

89034 contains a single copy of the cry1A.105 and cry2Ab2 expression 

cassettes at a single locus. No backbone sequences from plasmid PVZMIR245 or nptII coding sequences were detected in the corn genome. 

Southern blot analysis of DNA from several generations of MON 89034 

demonstrated the stability of the insert over seven generations. In addition, 

the DNA sequence of the insert and surrounding genomic sequences was 

determined using PCR and DNA sequencing; this analysis confirmed the 

organization of the elements within the insert and identified the 5’ and 3’ 

insert-to-genomic DNA junctions.

Classification: ACCEPTABLE 

46951402 

Expression 

levels/Assessment of the 

Cry1A.105 and 

Cry2Ab2 Protein Levels 

in Tissues of Insectprotected corn MON 

89034 Produced in 2005 

U.S. Field Trials

The levels of Cry1A.105 and Cry2Ab2 in corn tissues collected from MON 

89034 plants grown at five field sites in the U.S. were determined using 

enzyme-linked immunosorbent assays (ELISA). The means for Cry1A.105 

protein levels across all sites were 5.9 μg/g dry weight (dwt) in grain, 42 

μg/g dwt in forage, 12 μg/g dwt in pollen, 520 μg/g dwt in over season leaf 

collected at growth stage V2-V4 (OSL-1), 120 μg/g dwt in leaves OSL-4 

(collected at growth stage pre-VT), 12 μg/g dwt in forage root, and 50 μg/g 

dwt in stover. In tissues harvested throughout the growing season, mean 

Cry1A.105 protein levels across all sites ranged from 72-520 μg/g dwt in 

leaf, 42-79 μg/g dwt in root, and 100-380 μg/g dwt in whole plant. The 

means for Cry2Ab2 protein levels across all sites were 1.3 μg/g dwt in 

grain, 38 μg/g dwt in forage, 0.64 μg/g dwt in pollen, 180 μg/g dwt in OSL1, 160 μg/g dwt in OSL-4, 21 μg/g dwt in forage root, and 62 μg/g dwt in 

stover. In tissues harvested throughout the growing season, mean Cry2Ab2 

protein levels across all sites ranged from 130-180 μg/g dwt in leaf, 26-58 

μg/g dwt in root, and 39-130 μg/g dwt in whole plant. 

Classification: ACCEPTABLE

46951403
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

16

Study Type/Title Summary MRID #

Characterization of the 

active ingredient/ 

Characterization of the 

Cry2Ab2 Protein 

Purified from the Corn 

Grain of MON 89034 

and Comparison of the 

Physicochemical and 

Functional Properties of 

the Plant-Produced and 

E. coli-produced 

Cry2Ab2 Proteins

The physicochemical and functional properties of the plant-produced 

Cry2Ab2 were analyzed and compared with the properties of the E. coli

produced Cry2Ab2 using sodium dodecyl sulfate polyacrylamide gel 

electrophoresis (SDS-PAGE), western blot analysis, densitometry, matrix 

assisted laser desorption/ionization time of flight (MALDI-TOF) mass 

spectrometry, glycosylation analysis, and a diet-incorporation corn earworm 

(CEW) bioactivity assay. Similar immunoreactive bands migrating at 

approximately 61 kDa were observed in the plant-produced Cry2Ab2 and E. 

coli-produced reference samples. The plant-produced protein sample had an 

additional immunoreactive band migrating at approximately 50 kDa; Nterminal amino acid analysis of this protein indicated that it is a truncated 

Cry2Ab2 protein with its N-terminus starting at amino acid 145. MALDITOF mass spectrometry analysis of the ~61 and 50 kDa bands after trypsin 

digestion yielded peptide masses consistent with peptide masses of the 

predicted sequence of the Cry2Ab2 protein. Glycosylation analysis 

indicated that the Cry2Ab2 protein is not glycosylated. The plant-produced 

and E. coli-produced proteins gave similar results in the bioactivity assay: 

the mean EC50 values for the plant-produced Cry2Ab2 protein and E. coliproduced reference standard were both determined to be 0.16 μg Cry2Ab2 

per mL diet, with standard deviations of 0.04 and 0.01 μg Cry2Ab2 per mL 

diet, respectively. The results of this study demonstrate the structural and 

functional similarity between the plant-produced and the E. coli-produced 

Cry2Ab2 proteins. 

Classification: ACCEPTABLE

46951404 

Characterization of 

active 

ingredient/Evaluation of 

the Functional 

Equivalence of the 

Cry2Ab2 Protein 

Produced in E. coli and 

Bt Against a Sensitive 

Lepidopteran Species

The functional activity of purified Cry2Ab2 produced from E. coli and 

Cry2Ab2 produced from Bt was evaluated using a corn earworm larvae diet 

incorporation bioassay. There was no significant difference between the 

EC50 values (the effective concentration to inhibit growth of the target 

insect by 50%) for the two proteins, as shown by the large overlap in the 

95% confidence intervals and the nearly identical dose response curves. In 

addition, the two proteins showed the same rates of concentration-dependent 

growth inhibition, indicating that the proteins have the same mechanism of 

insecticidal action. 

Classification: ACCEPTABLE

46951405

Response to EPA 

Questions/ Responses to 

EPA Questions 

Regarding Applications 

524-LTL and 524-LTA 

to Register Insectprotected Corn MON 

89034 and MON 89034 

x MON 88017

In an email message from S. Cerrelli to N. Bogdanova dated April 23, 2007, 

EPA identified some deficiencies in the applications to register MON 89034 

and MON 89034 x MON 88017 and requested some additional information 

from Monsanto. In MRIDs 47127501-47127505, Monsanto responds to the 

questions and supplies the requested additional information. Monsanto’s 

responses are adequate. 

Classification: ACCEPTABLE

47127501-

47127505
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

17

B. HUMAN HEALTH ASSESSMENT OF Cry1A.105 

Section 408(c)(2)(A)(i) of the FFDCA allows EPA to establish an exemption from the 

requirement for a tolerance (the legal limit for a pesticide chemical residue in or on a food) only 

if EPA determines that the exemption is “safe.” Section 408(c)(2)(A)(ii) of the FFDCA defines 

“safe” to mean that “there is a reasonable certainty that no harm will result from aggregate 

exposure to the pesticide chemical residue, including all anticipated dietary exposures and all 

other exposures for which there is reliable information.” This includes exposure through 

drinking water and in residential settings, but does not include occupational exposure. Pursuant 

to section 408(c)(2)(B), in establishing or maintaining in effect an exemption from the 

requirement of a tolerance, EPA must take into account the factors set forth in section 

408(b)(2)(C), which require EPA to give special consideration to exposure of infants and 

children to the pesticide chemical residue in establishing a tolerance and to “ensure that there is a 

reasonable certainty that no harm will result to infants and children from aggregate exposure to 

the pesticide chemical residue... .” 

Additionally, section 408(b)(2)(D) of the FFDCA requires that the Agency consider “available 

information concerning the cumulative effects of a particular pesticide’s residues” and “other 

substances that have a common mechanism of toxicity.” EPA performs a number of analyses to 

determine the risks from aggregate exposure to pesticide residues. First, EPA determines the 

toxicity of pesticides. Second, EPA examines exposure to the pesticide through food, drinking 

water, and through other exposures that occur as a result of pesticide use in residential settings. 

1. Toxicological Profile 

Consistent with section 408(b)(2)(D) of the FFDCA, EPA has reviewed the available scientific 

data and other relevant information in support of this action and considered its validity, 

completeness and reliability, and the relationship of this information to human risk. EPA has 

also considered available information concerning the variability of the sensitivities of major 

identifiable subgroups of consumers, including infants and children. 

Mammalian Toxicity and Allergenicity Assessment

Monsanto submitted acute oral toxicity data demonstrating the lack of mammalian toxicity at 

high levels of exposure to the pure Cry1A.105 protein. These data demonstrate the safety of the 

product at a level well above maximum possible exposure levels that are reasonably anticipated 

in the crop. Basing this conclusion on acute oral toxicity data without requiring further toxicity 

testing and residue data is similar to the Agency position regarding toxicity testing and the 

requirement of residue data for the microbial Bacillus thuringiensis products from which this 

plant incorporated protectant was derived (See 40 CFR Sec. 158.740(b)(2)(i)). For microbial 

products, further toxicity testing and residue data are triggered by significant adverse acute 

effects in studies such as the mouse oral toxicity study, to verify the observed adverse effects and 

clarify the source of these effects (Tiers II & III). 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

18

An acute oral toxicity study in mice (MRID 46694603) indicated that Cry1A.105 is non-toxic to 

humans. Cry1A.105 produced from microbial culture was dosed by gavage as two doses 

separated by 4 hours (±20 minutes) to 10 females and 10 males (2072 mg/kg body weight). Two 

control groups were also included in the study: a bovine serum albumin protein control, and a 

vehicle control. One male in the test protein group was moribund and sacrificed on day 1 due to 

a mechanical dosing error; this death was not attributed to the test material. All other mice 

survived the study. There were no significant differences in body weight or body weight change 

among the three groups during the study, and no treatment-related gross pathological findings 

were observed. The oral LD50 for males, females, and combined mice was greater than 2072 

mg/kg. 

When proteins are toxic, they are known to act via acute mechanisms and at very low dose levels 

(Sjoblad, Roy D., et al., “Toxicological Considerations for Protein Components of Biological 

Pesticide Products,” Regulatory Toxicology and Pharmacology 15, 3-9 (1992)). Therefore, since 

no acute effects were shown to be caused by Cry1A.105, even at relatively high dose levels, the 

Cry1A.105 protein is not considered toxic. Further, amino acid sequence comparisons showed 

no similarities between the Cry1A.105 and known toxic proteins in protein databases that would 

raise a safety concern. 

Since Cry1A.105 is a protein, allergenic potential was also considered. Currently, no definitive 

tests for determining the allergenic potential of novel proteins exist. Therefore, EPA uses a 

weight-of-evidence approach where the following factors are considered: source of the trait; 

amino acid sequence comparison with known allergens; and biochemical properties of the 

protein, including in-vitro digestibility in simulated gastric fluid (SGF) and glycosylation. This 

approach is consistent with the approach outlined in the Annex to the Codex Alimentarius 

“Guideline for the Conduct of Food Safety Assessment of Foods Derived from RecombinantDNA Plants.” The allergenicity assessment for Cry1A.105 follows: 

1. Source of the trait. Bacillus thuringiensis is not considered to be a source of 

allergenic proteins. 

2. Amino acid sequence. A comparison of the amino acid sequence of Cry1A.105 with 

known allergens showed no overall sequence similarity or identity at the level of 

eight contiguous amino acid residues. 

3. Digestibility. The Cry1A.105 protein was digested within 30 seconds in simulated 

gastric fluid containing pepsin. 

4. Glycosylation. Cry1A.105 expressed in corn was shown not to be glycosylated. 

5. Conclusion. Considering all of the available information, EPA has concluded that the 

potential for Cry1A.105 to be a food allergen is minimal. 

Although Cry1A.105 was only shown not to be glycosylated in corn, it is unlikely to be 

glycosylated in any other crops because in order for a protein to be glycoslyated, it needs to 

contain specific recognition sites for the enzymes involved in glycosylation, and the mechanisms 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

19

of protein glycosylation are similar in different plants (Lerouge, P. Cabanes-Macheteau, M., 

Rayon, C., Fichette-Lainé, A-C., Gomord, V., and Faye, L., “N-Glycoprotein biosynthesis in 

plants: recent developments and future trends,” Plant Molecular Biology 38: 31-48, 1998). 

2. Aggregate Exposures 

Pursuant to FFDCA section 408(b)(2)(D)(vi), EPA considers available information concerning 

aggregate exposures from the pesticide residue in food and all other non-occupational exposures, 

including drinking water from ground water or surface water and exposure through pesticide use 

in gardens, lawns, or buildings (residential and other indoor uses). 

The Agency has considered available information on the aggregate exposure levels of consumers 

(and major identifiable subgroups of consumers) to the pesticide chemical residue and to other 

related substances. These considerations include dietary exposure under the tolerance exemption 

and all other tolerances or exemptions in effect for residues of the plant-incorporated protectants, 

and exposure from non-occupational sources. Exposure via the skin or inhalation is not likely, 

since the plant incorporated protectant is contained within plant cells, which essentially 

eliminates these exposure routes or reduces these exposure routes to negligible. In addition, even 

if exposure can occur through inhalation, the potential for Cry1A.105 to be an allergen is low, as 

discussed previously. Although the allergenicity assessment focused on the Cry1A.105 protein’s 

potential to be a food allergen, the data also indicated a low potential for Cry1A.105 to be an 

inhalation allergen. Exposure to infants and children via residential or lawn use is not expected, 

because the use sites for the Cry1A.105 protein is agricultural. Oral exposure, at very low levels, 

may occur from ingestion of processed corn products and, theoretically, drinking water. 

However oral toxicity testing in mammals showed no adverse effects. 

3. Cumulative Effects 

Pursuant to FFDCA section 408(b)(2)(D)(v), EPA has considered available information on the 

cumulative effects of such residues and other substances that have a common mechanism of 

toxicity. These considerations included the cumulative effects on infants and children of such 

residues and other substances with a common mechanism of toxicity. Because there is no 

indication of mammalian toxicity from the plant-incorporated protectant, EPA concluded that 

there are no cumulative effects for the Cry1A.105 protein. 

4. Determination of Safety for U.S. Population, Infants and Children 

a) Toxicity and Allergenicity Conclusions 

The data submitted and cited regarding potential health effects for the Cry1A.105 protein 

included the characterization of the expressed Cry1A.105 protein in corn, as well as the acute 

oral toxicity study, amino acid sequence comparisons to known allergens and toxins, and in vitro 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

20

digestibility of the protein. The results of these studies were used to evaluate human risk, and the 

validity, completeness, and reliability of the available data from the studies were also considered. 

 

Adequate information was submitted to show that the Cry1A.105 test material derived from 

microbial culture was biochemically and functionally equivalent to the protein produced by the 

plant-incorporated protectant ingredient in the plant. Microbially produced protein was used in 

the studies so that sufficient material for testing was available. 

The acute oral toxicity data submitted support the prediction that the Cry1A.105 protein would 

be non-toxic to humans. As mentioned above, when proteins are toxic, they are known to act via 

acute mechanisms and at very low dose levels. Given that no treatment-related adverse effects 

were shown to be caused by the Cry1A.105 protein, even at relatively high dose levels, the 

Cry1A.105 protein is not considered toxic. Basing this conclusion on acute oral toxicity data 

without requiring further toxicity testing or residue data is similar to the Agency position 

regarding toxicity and the requirement of residue data for the microbial Bacillus thuringiensis

products from which this plant-incorporated protectant was derived (See 40 CFR 

158.740(b)(2)(i)). For microbial products, further toxicity testing and residue data are triggered 

when significant adverse effects are seen in studies such as the acute oral toxicity study. Further 

studies verify the observed adverse effects and clarify the source of these effects (Tiers II and 

III). 

Residue chemistry data were not required for a human health effects assessment of the subject 

plant-incorporated protectant ingredients because of the lack of mammalian toxicity. Data 

submitted by the applicant, however, demonstrated low levels of Cry1A.105 in corn tissues. 

Since Cry1A.105 is a protein, potential allergenicity is also considered as part of the toxicity 

assessment. Considering all of the available information (1) Cry1A.105 originates from a nonallergenic source; (2) Cry1A.105 has no sequence similarities with known allergens; (3) 

Cry1A.105 is not glycosylated; and (4) Cry1A.105 is rapidly digested in simulated gastric fluid; 

EPA has concluded that the potential for Cry1A.105 to be a food allergen is minimal. 

The Agency did not evaluate information concerning the dietary consumption patterns of 

consumers (and major identifiable subgroups of consumers including infants and children) or 

apply safety factors that are generally recognized as appropriate when animal experimentation 

data are used to assess risks to humans. The lack of mammalian toxicity at high levels of 

exposure to the Cry1A.105 protein, as well as the minimal potential to be a food allergen, 

satisfactorily demonstrated the safety of the products at levels well above the anticipated 

maximum exposure levels. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

21

The genetic material necessary for the production of the plant-incorporated protectant active 

ingredient include the nucleic acids (DNA, RNA) that encode these proteins and regulatory 

regions. The genetic material (DNA, RNA), necessary for the production of the Cry1A.105 

protein has been exempted from the requirement of a tolerance under 40 CFR 174.507 “Nucleic 

acids that are part of a plant-incorporated protectant .” 

b) Infants and Children Risk Conclusions 

FFDCA section 408(b)(2)(C) provides that EPA shall assess the available information about 

consumption patterns among infants and children, special susceptibility of infants and children to 

pesticide chemical residues, and the cumulative effects on infants and children of the residues 

and other substances with a common mechanism of toxicity. In addition, FFDCA section 

408(b)(2)(C) also provides that EPA shall apply an additional tenfold margin of safety for infants 

and children in the case of threshold effects to account for prenatal and postnatal toxicity and the 

completeness of the database unless EPA determines that a different margin of safety will be safe 

for infants and children. 

In this instance, based on all the available information, the Agency concluded that there is a 

finding of no toxicity for the Cry1A.105 protein. Thus, there are no threshold effects of concern 

and, as a result, the provision requiring an additional margin of safety does not apply. Further, 

the considerations of consumption patterns, special susceptibility, and cumulative effects do not 

apply. 

 

c) Overall Safety Conclusion 

There is a reasonable certainty that no harm will result from aggregate exposure to the U.S. 

population, including infants and children, to the Cry1A.105 protein and the genetic material 

necessary for its production. This includes all anticipated dietary exposures and all other 

exposures for which there is reliable information. The Agency has arrived at this conclusion 

because, as previously discussed, no toxicity to mammals has been observed, nor any indication 

of allergenicity potential for this plant-incorporated protectant. 

5. Other Considerations 

a) Endocrine Disruptors 

The pesticidal active ingredient is a protein, derived from a source that is not known to exert an 

influence on the endocrine system. Therefore, the Agency is not requiring information on the 

endocrine effects of the plant-incorporated protectant at this time. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

22

b) Analytical Method(s) 

A standard operating procedure for an enzyme-linked immunosorbent assay for the detection and 

quantification of Cry1A.105 in corn tissue has been submitted. 

c) Codex Maximum Residue Level 

No Codex maximum residue level exists for the plant-incorporated protectant Bacillus 

thuringiensis Cry1A.105 protein. 

The human health studies submitted for Cry1A.105 are summarized in Table 3 below. 

Table 3. Summary of Cry1A.105 Human Health Data 

Study Type/Title Summary MRID #

Acute oral toxicity 

(OPPTS 870.1100)/

Acute Oral Toxicity 

Study in Mice with 

Cry1A.105 Protein2

The Cry1A.105 test protein (2072 mg/kg body weight) was dosed by gavage 

as two doses separated by 4 hours (±20 minutes). The BSA protein control 

(1998 mg/kg body weight) was dosed using the same procedure as for the 

test protein group. The vehicle control group was dosed with carbonatebicarbonate with reduced glutathione. Body weight was recorded prior to 

fasting, prior to dosing, and on days 7 and 14. The test animals were 

observed for clinical signs of toxicity two times post-dosing and for 14 days. 

A general health/mortality check was done twice daily. All animals were 

necropsied. One male in the test protein group was moribund and sacrificed 

on day 1 due to a mechanical dosing error, which resulted in a perforated 

esophagus. All other mice survived the study. There were no significant 

differences in body weight or body weight change among the three groups 

during the study. The oral LD50 for males, females, and combined mice was 

greater than 2072 mg/kg. This places Cry1A.105 Protein in TOXICITY 

CATEGORY III due to dose amounts only; no signs of toxicity were 

observed. 

Classification: ACCEPTABLE

46694603 

Amino acid sequence 

comparison/

Bioinformatics Analysis 

of the Cry1A.105 

Protein Utilizing the 

AD6, Toxin5, and 

Allpeptides Databases 2 

Bioinformatic analyses were used to search for sequence similarities 

between the Cry1A.105 protein and toxins and allergens. The FASTA 

alignment tool and the allergen (AD5), toxin (TOXIN5), and public domain 

(ALLPEPTIDES) database sequences were used to assess structural 

similarity. No significant similarities were found, other than with the 

Cry1Ac protein; this alignment is not surprising, since the Cry1A.105 

protein contains a significant portion of the Cry1Ac protein. The 

Cry1A.105 protein sequence was also screened against the AD5 sequence 

database using a pair-wise comparison algorithm. No matches of 8 amino 

acids or more were found for the Cry1A.105 protein in the AD5 database. 

No similarities between Cry1A.105 protein and known allergens, human or 

animal toxins, or pharmacologically active proteins were found in the study. 

Classification: ACCEPTABLE 

46694605 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

23

Study Type/Title Summary MRID #

In vitro digestibility/

Assessment of the In 

Vitro Digestibility of 

the Cry1A.105 Protein 

in Simulated Gastric 

Fluid2

No bands representative of intact Cry1A.105 protein were identified by 

SDS-PAGE after ≥30 seconds incubation with simulated gastric fluid 

containing pepsin. A very faint band of 4.5 kDaltons was observed between 

the 30 second and 20 minute digestions but was not observed after 20 

minutes. The limit of detection for the SDS-PAGE method was determined 

to be 5 ng for the full-length Cry1A.105 protein. Both the pepsin stability 

and test material stability controls gave appropriate responses. In the 

Western Blot assay, Cry1A.105 was not immunologically identifiable within 

30 seconds of incubation. The limit of detection for the method was 

determined to be 1 ng for the full-length Cry1A.105 protein. The pepsin 

stability and test material stability controls gave appropriate responses. 

Classification: ACCEPTABLE 

46694606 

Heat stability/

Immunodetection of 

Cry2Ab2 and 

Cry1A.105 Proteins in 

Corn Grain from MON 

89034 Following Heat 

Treatment 2

The immunodetectability of the Cry1A.105 and Cry2Ab2 proteins in corn 

grain from MON 89034 following heat treatment was assessed. MON 

89034 and conventional grain were ground, mixed with water, and then 

heated in an oven at 204 oC for 20 minutes to simulate the heating process 

used commercially to process grain. Heated and unheated grain was 

extracted with two buffers: 50 mM CAPS and 50 mM NLS (CAPS 

containing 2% N-Lauroyl sarcosine). The extracts were analyzed using 

western blot to detect the presence of the Cry1A.105 and Cry2Ab2 proteins. 

The amount of immunodetectable Cry2Ab2 protein in either CAPS or NLS 

buffer extracts of MON 89034 after heating was below the lower LOD. 

Based on the LOD and the estimated amount of protein in the unheated 

extract, it was determined that the Cry2Ab2 protein decreased at least 77% 

and 70%, respectively following heat treatment, relative to their original 

values. Likewise, the amount of immunodetectable Cry1A.105 protein in 

either CAPS or NLS buffer extracts of MON 89034 after heating was below 

the limit of detection (LOD) and had decreased by at least 94% and 78%, 

respectively, relative to their original values. This loss is likely due to 

protein degradation or aggregation into an insoluble complex as a result of 

heat treatment. 

Classification: ACCEPTABLE 

46694607 

Amino acid sequence 

comparison/

Bioinformatics Analysis 

of the Cry1A.105 

Protein Utilizing the 

AD6, Toxin5, and 

Allpeptides Databases 

The amino acid sequence of the Cry1A.105 protein was compared to the 

sequences of known allergens and toxins using allergen (AD6), toxin 

(TOXIN5), and public domain (ALLPEPTIDES) databases and the FASTA 

algorithm. In addition to the FASTA comparisons, the Cry1A.105 protein 

sequence was compared to the AD6 database using 8 amino acid sliding 

blocks and the ALLERGENSEARCH algorithm. No proteins were 

identified with an E score of less than 1x10-5 from the search using the AD6 

database, indicating that Cry1A.105 has no structural similarity to any 

known allergens, gliadins, or glutenins. In addition, no matches of 8 or 

more amino acids were found between the Cry1A.105 protein and sequences 

in the AD6 database. In the comparisons using the TOXIN5 and 

ALLPEPTIDES databases, the highest similarity identified was with 

Bacillus thuringiensis pesticidal protein Cry1Ac (92% identity over an 

1,182 aa window and an E score of 0). This result is not surprising, given 

that the Cry1A.105 protein contains a significant portion of the Cry1Ac 

protein. No significant similarities between Cry1A.105 protein and known 

46951410 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

24

Study Type/Title Summary MRID #

allergens, human or animal toxins, or pharmacologically active proteins 

were found in this study. 

Classification: ACCEPTABLE 

In vitro digestibility/ 

Assessment of the in 

vitro Digestibility of the 

Cry1A.105 Protein in 

Simulated Intestinal 

Fluid 

The in vitro digestibility of Cry1A.105 in simulated intestinal fluid 

containing pancreatin was investigated using western blot analysis. The 

band for the full-length Cry1A.105 was below the LOD in the 5 minute 

time-point sample and in the later time-point samples. The LOD for the 

full-length Cry1A.105 protein by western blot analysis was estimated to be 

0.1 ng, which was 0.5 % of the total protein loaded. Therefore, at least 

99.5% of the full-length protein was digested within 5 minutes. Bands from 

proteolytic fragments with approximate molecular weights of 60, 32, and 30 

kDa were visible in the five-minute time-point sample. The ~32 kDa 

fragment was digested and undetectable at the 2-hour time-point and after. 

The ~30 kDa fragment, which appears as a doublet, was still visible in the 

24-hour time-point sample, but its intensity decreased substantially. The 

~60 kDa fragment, which also appears as a doublet, represents the trypsinresistant core and appears to be fairly stable throughout the 24-hour 

digestion experiment. 

Classification: ACCEPTABLE

46951408 

C. HUMAN HEALTH ASSESSMENT Cry2Ab2

Section 408(c)(2)(A)(i) of the FFDCA allows EPA to establish an exemption from the 

requirement for a tolerance (the legal limit for a pesticide chemical residue in or on a food) only 

if EPA determines that the exemption is “safe.” Section 408(c)(2)(A)(ii) of the FFDCA defines 

“safe” to mean that “there is a reasonable certainty that no harm will result from aggregate 

exposure to the pesticide chemical residue, including all anticipated dietary exposures and all 

other exposures for which there is reliable information.” This includes exposure through 

drinking water and in residential settings, but does not include occupational exposure. Pursuant 

to section 408(c)(2)(B), in establishing or maintaining in effect an exemption from the 

requirement of a tolerance, EPA must take into account the factors set forth in section 

408(b)(2)(C), which require EPA to give special consideration to exposure of infants and 

children to the pesticide chemical residue in establishing a tolerance and to “ensure that there is a 

reasonable certainty that no harm will result to infants and children from aggregate exposure to 

the pesticide chemical residue... .” 

Additionally, section 408(b)(2)(D) of the FFDCA requires that the Agency consider “available 

information concerning the cumulative effects of a particular pesticide’s residues” and “other 

substances that have a common mechanism of toxicity.” EPA performs a number of analyses to 

determine the risks from aggregate exposure to pesticide residues. First, EPA determines the 

toxicity of pesticides. Second, EPA examines exposure to the pesticide through food, drinking 

water, and through other exposures that occur as a result of pesticide use in residential settings. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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1. Toxicological Profile 

Consistent with section 408(b)(2)(D) of the FFDCA, EPA has reviewed the available scientific 

data and other relevant information in support of this action and considered its validity, 

completeness and reliability and the relationship of this information to human risk. EPA has also 

considered available information concerning the variability of the sensitivities of major 

identifiable subgroups of consumers, including infants and children. 

Mammalian Toxicity and Allergenicity Assessment

Monsanto has submitted acute oral toxicity data demonstrating the lack of mammalian toxicity at 

high levels of exposure to the pure Cry2Ab2 protein. These data demonstrate the safety of the 

product at a level well above maximum possible exposure levels that are reasonably anticipated 

in the crop. Basing this conclusion on acute oral toxicity data without requiring further toxicity 

testing and residue data is similar to the position regarding toxicity testing and the requirement of 

residue data for the Agency’s microbial Bacillus thuringiensis products from which this plantincorporated protectant was derived (See 40 CFR Sec. 158.740(b)(2)(i)). For microbial products, 

further toxicity testing (Tiers II & III) and residue data are triggered by significant adverse acute 

effects in studies such as the acute oral toxicity study, to verify the observed adverse effects and 

clarify the source of these effects. 

An acute oral toxicity study in mice (MRID 44966602) indicated that Cry2Ab2 is non-toxic to 

humans. Three groups of ten male and ten female mice were dosed by oral gavage with 30, 300, 

or 1000 mg/kg bodyweight of microbially produced Cry2Ab2 protein. Two negative control 

groups were also included in the study: bovine serum albumin protein control, and a vehicle 

control (purified water). There were no significant differences between the test and control 

groups; therefore, the Cry2Ab2 protein does not appear to cause any significant adverse effects 

at an exposure level of up to 1000 mg/kg bodyweight. 

When proteins are toxic, they are known to act via acute mechanisms and at very low dose 

levels. Therefore, given that no acute effects were shown to be caused by Cry2Ab2, even at 

relatively high dose levels, the Cry2Ab2 protein is not considered toxic. Further, amino acid 

sequence comparisons showed no similarities between the Cry2Ab2 protein and known toxic 

proteins in protein databases that would raise a safety concern. 

Since Cry2Ab2 is a protein, allergenic potential was also considered. Currently, no definitive 

tests for determining the allergenic potential of novel proteins exist. Therefore, EPA uses a 

weight-of- evidence approach where the following factors are considered: source of the trait; 

amino acid sequence comparison with known allergens; and biochemical properties of the 

protein, including in vitro digestibility in simulated gastric fluid (SGF) and glycosylation. This 

approach is consistent with the approach outlined in the Annex to the Codex Alimentarius 

“Guideline for the Conduct of Food Safety Assessment of Foods Derived from RecombinantDNA Plants.” The allergenicity assessment for Cry2Ab2 follows: 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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1. Source of the trait. Bacillus thuringiensis is not considered to be a source of allergenic 

proteins. 

2. Amino acid sequence. A comparison of the amino acid sequence of Cry2Ab2 with 

known allergens showed no significant overall sequence similarity or identity at the level 

of eight contiguous amino acid residues. 

3. Digestibility. The Cry2Ab2 protein was digested within 15 seconds in simulated gastric 

fluid containing pepsin. 

4. Glycosylation. Cry2Ab2 expressed in corn was shown not to be glycosylated. 

5. Conclusion. Considering all of the available information, EPA has concluded that the 

potential for Cry2Ab2 to be a food allergen is minimal. 

Although Cry2Ab2 was only shown not to be glycosylated in corn, it is unlikely to be 

glycosylated in any other crops because in order for a protein to be glycoslyated, it must contain 

specific recognition sites for the enzymes involved in glycosylation, and the mechanisms of 

protein glycosylation are similar in different plants (Lerouge, P. Cabanes-Macheteau, M., Rayon, 

C., Fichette-Lainé, A-C., Gomord, V., and Faye, L., “N-Glycoprotein biosynthesis in plants: 

recent developments and future trends,” Plant Molecular Biology 38: 31-48, 1998). 

2. Aggregate Exposures 

Pursuant to FFDCA section 408(b)(2)(D)(vi), EPA considers available information concerning 

aggregate exposures from the pesticide residue in food and all other non-occupational exposures, 

including drinking water from ground water or surface water and exposure through pesticide use 

in gardens, lawns, or buildings (residential and other indoor uses). The Agency considered 

available information on the aggregate exposure levels of consumers (and major identifiable 

subgroups of consumers) to the pesticide chemical residue and to other related substances. 

These considerations include dietary exposure under the tolerance exemption and all other 

tolerances or exemptions in effect for the plant incorporated protectant’s chemical residue, and 

exposure from non-occupational sources. Exposure via the skin or inhalation is not likely since 

the plant incorporated protectant is contained within plant cells, which essentially eliminates 

these exposure routes or reduces exposure by these routes to negligible. In addition, even if 

exposure can occur through inhalation, the potential for Cry2Ab2 protein to be an allergen is 

low, as previously discussed. Although the allergenicity assessment focused on Cry2Ab2 

protein’s potential to be a food allergen, the data also indicated a low potential for Cry2Ab2 to be 

an inhalation allergen. Exposure to infants and children via residential or lawn use is also not 

expected because the use sites for the Cry2Ab2 protein is agricultural. Oral exposure, at very 

low levels, may occur from ingestion of processed corn products and, theoretically, drinking 

water. However, oral toxicity testing in laboratory mammals showed no adverse effects. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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3. Cumulative Effects 

Pursuant to FFDCA section 408(b)(2)(D)(v), EPA has considered available information on the 

cumulative effects of such residues and other substances that have a common mechanism of 

toxicity. These considerations included the cumulative effects on infants and children of such 

residues and other substances with a common mechanism of toxicity. Because there is no 

indication of mammalian toxicity from the plant incorporated protectant, we conclude that there 

are no cumulative effects for the Cry2Ab2 protein. 

4. Determination of Safety for U.S. Population, Infants and Children 

Toxicity and Allergenicity Conclusions 

The data submitted and cited regarding potential health effects for the Cry2Ab2 protein included 

the characterization of the expressed Cry2Ab2 protein in corn, as well as the acute oral toxicity 

study, amino acid sequence comparisons to known allergens and toxins, and in vitro digestibility 

of the protein. The results of these studies were used to evaluate human risk, and the validity, 

completeness, and reliability of the available data from the studies were also considered. 

 

Adequate information was submitted to show that the Cry2Ab2 test material derived from 

microbial culture was biochemically and functionally equivalent to the protein in the plant. 

Microbially produced protein was used in the safety studies so that sufficient material for testing 

was available. 

The acute oral toxicity data submitted by Monsanto support the prediction that the Cry2Ab2 

protein would be non-toxic to humans. As mentioned above, when proteins are toxic, they are 

known to act via acute mechanisms and at very low dose levels. Given that no treatment-related 

adverse effects were shown to be caused by the Cry2Ab2 protein, even at relatively high dose 

levels, the Cry2Ab2 protein is not considered toxic. Basing this conclusion on acute oral toxicity 

data without requiring further toxicity testing and residue data is similar to the Agency position 

regarding toxicity and the requirement of residue data for the microbial Bacillus thuringiensis

products from which this plant-incorporated protectant was derived (See 40 CFR 

158.740(b)(2)(i)). For microbial products, further toxicity testing and residue data are triggered 

when significant adverse effects are seen in studies such as the acute oral toxicity study. Further 

studies verify the observed adverse effects and clarify the source of these effects. 

Residue chemistry data were not required for a human health effects assessment of the subject 

plant-incorporated protectant ingredients because of the lack of mammalian toxicity. Data 

submitted by the applicant however, demonstrated low levels of the Cry2Ab2 in corn tissues. 

Since Cry2Ab2 is a protein, potential allergenicity is also considered as part of the toxicity 

assessment. Considering that (1) Cry2Ab2 originates from a non-allergenic source, (2) Cry2Ab2 

has no sequence similarities with known allergens, (3) Cry2Ab2 is not glycosylated, and (4) 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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Cry2Ab2 is rapidly digested in simulated gastric fluid, EPA concluded that the potential for 

Cry2Ab2 to be a food allergen is minimal. 

The Agency did not consider information concerning the dietary consumption patterns of 

consumers (and major identifiable subgroups of consumers including infants and children) or 

safety factors that are generally recognized as appropriate when animal experimentation data are 

used to assess risks to humans. The lack of mammalian toxicity at high levels of exposure to the 

Cry2Ab2 protein, as well as the minimal potential to be a food allergen, demonstrate the safety 

of the product at levels well above possible maximum exposure levels anticipated in the crop. 

The genetic material necessary for the production of the plant-incorporated protectant active 

ingredient include the nucleic acids (DNA, RNA) that encode these proteins and regulatory 

regions. The genetic material (DNA, RNA) necessary for the production of the Cry2Ab2 protein 

has been exempted from the requirement of a tolerance under 40 CFR 174.507 “Nucleic acids 

that are part of a plant-incorporated protectant.” 

 

a) Infants and Children Risk Conclusions 

FFDCA section 408(b)(2)(C) provides that EPA shall assess the available information about 

consumption patterns among infants and children, special susceptibility of infants and children to 

pesticide chemical residues and the cumulative effects on infants and children of the residues and 

other substances with a common mechanism of toxicity. In addition, FFDCA section 

408(b)(2)(C) also provides that EPA shall apply an additional tenfold margin of safety for infants 

and children in the case of threshold effects to account for prenatal and postnatal toxicity and the 

completeness of the database unless EPA determines that a different margin of safety will be safe 

for infants and children. 

In this instance, based on all the available information, the Agency concluded that there is a 

finding of no toxicity for the Cry2Ab2 protein. Thus, there are no threshold effects of concern 

and, as a result, the provision requiring an additional margin of safety does not apply. Further, 

the considerations of consumption patterns, special susceptibility, and cumulative effects do not 

apply. 

 

b) Overall Safety Conclusion 

There is a reasonable certainty that no harm will result from aggregate exposure to the U.S. 

population, including infants and children, to the Cry2Ab2 protein and the genetic material 

necessary for its production. This includes all anticipated dietary exposures and all other 

exposures for which there is reliable information. The Agency has arrived at this conclusion 

because, as discussed above, no toxicity to mammals has been observed, nor any indication of 

allergenicity potential for the plant-incorporated protectant. 
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5. Other Considerations 

a) Endocrine Disruptors 

The pesticidal active ingredient is a protein, derived from a source that is not known to exert an 

influence on the endocrine system. Therefore, the Agency is not requiring information on the 

endocrine effects of this plant-incorporated protectant at this time. 

b) Analytical Method(s) 

A protocol for an enzyme-linked immunosorbent assay for the detection and quantification of 

Cry2Ab2 in corn tissue has been submitted, and a commercially available qualitative 

immunochromatographic test strip was shown to detect the Cry2Ab2 protein in corn tissues. 

c) Codex Maximum Residue Level 

No Codex maximum residue level exists for the plant-incorporated protectant Bacillus 

thuringiensis Cry2Ab2 protein and the genetic material necessary for its production in corn. 
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Table 4. Summary of Cry2Ab2 Human Health Data 

Study Type/Title Summary MRID #

Acute oral toxicity 

(OPPTS 870.1100)/ An 

Acute Oral Toxicity 

Study in Mice with 

Cry2Ab2 Protein

The acute oral toxicity of the Cry2Ab2 protein was assessed in CD-1 mice. 

Ten male and 10 female mice received E. coli-produced Cry2Ab2 protein at 

a dose of 2198 mg/kg by oral gavage in two doses (test protein group); ten 

male and 10 female mice were treated with 2 mM carbonate-bicarbonate, 

2mM reduced glutathione buffer (vehicle control group); and ten male and 

10 female mice received bovine serum albumin at a dose of 2424 mg/kg 

(protein control group). Body weight was recorded prior to fasting, prior to 

dosing, and on days 7 and 14. The test animals were observed for clinical 

signs of toxicity two times post-dosing and daily for 14 days. A general 

health/mortality check was done twice daily. All animals were euthanized 

and necropsied on day 14. All mice survived the study. There were no 

significant differences in body weight or body weight change among the 

three groups during the study. The oral LD50 for males, females, and 

combined mice was greater than 2198 mg/kg. This places Cry2Ab2 protein 

in TOXICITY CATEGORY III because of dose amounts only; no signs of 

toxicity were observed. 

Classification: ACCEPTABLE 

46951406 

In vitro 

digestibility/Assessment 

of the in vitro

digestibility of the 

Cry2Ab2 protein in 

simulated gastric fluid

The in vitro digestibility of Cry2Ab2 in simulated gastric fluid was 

investigated. No bands representative of intact Cry2Ab2 protein were 

identified by SDS-PAGE or western blot analysis after ≥ 30 seconds 

incubation with simulated gastric fluid containing pepsin. In the stained gel, 

a faint band with molecular weight ~5 kDa was visible in the 30-second 

time-point, but not in any other samples. No proteolytic fragments were 

observed in the western blot. The limit of detection (LOD) for the fulllength Cry2Ab2 protein by SDS-PAGE with staining was determined to be 

5 ng or approximately 0.6% of the total protein loaded. Therefore, at least 

99.4% of the full-length Cry2Ab2 protein was digested within 30 seconds. 

The LOD for the full-length Cry2Ab2 protein by western blot analysis was 

determined to be 0.2 ng or 1% of the total protein loaded. Based on the fact 

that no band was observed for the Cry2Ab2 protein in the 30 second timepoint sample in the western blot and the LOD for the protein using this 

method, it was concluded that at least 99% of the Cry2Ab2 protein was 

digested within 30 seconds. 

Classification: ACCEPTABLE 

46951407 

In vitro digestibility/

Assessment of the in 

vitro Digestibility of the 

Cry2Ab2 Protein in 

Simulated Intestinal 

Fluid

The in vitro digestibility of Cry2Ab2 in simulated intestinal fluid containing 

pancreatin was investigated using western blot analysis. The band for the 

full-length Cry2Ab2 was below the LOD in the 15 minute time-point sample 

and in the later time-point samples. The LOD for the full-length Cry2Ab2 

protein by western blot analysis was estimated to be 0.5 ng, which was 2.5% 

of the total protein loaded. Therefore, at least 97.5% of the full-length 

protein was digested within 15 minutes. Bands from proteolytic fragments 

with approximate molecular weights of 60, 55, 50, 40, 12, and 10 kDa were 

visible in the five-minute time-point sample. The bands for all of these 

proteolytic fragments except for the 50 kDa fragment were undetectable at 

46951409 
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Study Type/Title Summary MRID #

the 24-hour incubation time-point. 

Classification: ACCEPTABLE 

Heat stability/

Immunodetection of 

Cry2Ab2 and 

Cry1A.105 Proteins in 

Corn Grain from MON 

89034 Following Heat 

Treatment 2

The immunodetectability of the Cry1A.105 and Cry2Ab2 proteins in corn 

grain from MON 89034 following heat treatment was assessed. MON 

89034 and conventional grain were ground, mixed with water, and then 

heated in an oven at 204 oC for 20 minutes to simulate the heating process 

used commercially to process grain. Heated and unheated grain was 

extracted with two buffers: 50 mM CAPS and 50 mM NLS (CAPS 

containing 2% N-Lauroyl sarcosine). The extracts were analyzed using 

western blot to detect the presence of the Cry1A.105 and Cry2Ab2 proteins. 

The amount of immunodetectable Cry2Ab2 protein in either CAPS or NLS 

buffer extracts of MON 89034 after heating was below the lower LOD. 

Based on the LOD and the estimated amount of protein in the unheated 

extract, it was determined that the Cry2Ab2 protein decreased at least 77% 

and 70%, respectively following heat treatment, relative to their original 

values. Likewise, the amount of immunodetectable Cry1A.105 protein in 

either CAPS or NLS buffer extracts of MON 89034 after heating was below 

the limit of detection (LOD) and had decreased by at least 94% and 78%, 

respectively, relative to their original values. This loss is likely due to 

protein degradation or aggregation into an insoluble complex as a result of 

heat treatment. 

Classification: ACCEPTABLE 

46694607 

Amino acid sequence 

comparison/ Bioinformatic 

Evaluation of the Cry2Ab2 

Protein Utilizing the AD6, 

TOXIN5, and 

ALLPEPTIDES Databases 

The amino acid sequence of the Cry2Ab2 protein was compared to the 

sequences of known allergens and toxins using allergen (AD6), toxin 

(TOXIN5), and public domain (ALLPEPTIDES) databases and the FASTA 

algorithm. In addition to the FASTA comparisons, the Cry2Ab2 protein 

sequence was compared to the AD6 database using 8 amino acid sliding 

blocks and the ALLERGENSEARCH algorithm. No proteins were 

identified with an E score of less than 1x10-5 from the search using the AD6 

database, indicating that Cry2Ab2 has no structural similarity to any known 

allergens, gliadins, or glutenins. In addition, no matches of 8 amino acids 

were found between the Cry2Ab2 protein and sequences in the AD6 

database. In the comparisons using the TOXIN5 and ALLPEPTIDES 

databases, the highest similarities identified were with Cry protein 

homologues derived from Bacillus thuringiensis, Clostridium bifermentans, 

Paenibacillus popilliae, and Paenibacillus lentimorbus. The results indicate 

that the Cry2Ab2 protein does not share sequence homology with any 

proteins that may have adverse effects in humans or animals. 

46951411
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D. ENVIRONMENTAL ASSESSMENT for MON 89034 

Background 

Monsanto has requested a registration for Bacillus thuringiensis Cry1A.105 and Cry2Ab2 

proteins and the genetic material necessary for their production in all corn lines and varieties. 

The nptII selectable marker gene was used in the transformation process, but was isolated and 

removed from transformed plants via traditional breeding. The result is marker-free MON 89034 

corn. The Cry proteins expressed in this event are intended to control the lepidopteran pests 

European corn borer (ECB, Ostrinia nubilalis), corn ear worm (CEW, Helicoverpa zea), fall 

army worm (FAW, Spodoptera frugiperda), and black cutworm (BCW, Agrotis ipsilon) which 

are primary pests of corn in the United States. These pests feed on the base of seedlings and on 

the stalk, leaf, and ear tissue of corn plants, thereby destroying the entire plant, weakening the 

stalk, and/or damaging the ear. In areas where one or more of these pests is prevalent, significant 

financial losses are realized from decreased corn yields and increased expenditures on chemical 

pest control agents, including organophosphate, carbamate and pyrethroid insecticides. 

EPA has conducted an environmental risk assessment of Cry1A.105, Cry2Ab2 and MON 89034 

when expressed in corn. General topics covered in this assessment include effects on wildlife, 

gene flow to related wild plants and its potential effects, and fate of these Cry proteins in the 

environment. This assessment is based on data submitted to EPA during the development of 

Event MON 89034 corn lines, additional data submitted for registration, Federal Insecticide 

Fungicide and Rodenticide Act (FIFRA) Scientific Advisory Panel (SAP) recommendations, 

consultations with scientific experts, and public comments on Plant-incorporated Protectant 

(PIP) regulation. 

1. Tiered Testing Hazard and Risk Assessment Process 

To minimize data requirements and avoid unnecessary tests, risk assessments are structured such 

that risk is determined first from estimates of hazard under “worst-case” exposure conditions. A 

lack of adverse effects under these conditions would provide enough confidence that there is no 

risk and no further data would be needed. Hence, such screening tests conducted early in an 

investigation tend to be broad in scope but relatively simple in design, and can be used to 

demonstrate acceptable risk under most conceivable conditions. When screening studies suggest 

potentially unacceptable risk, additional studies are designed to assess risk under more realistic 

field exposure conditions. These later tests are more complex than earlier screening studies. Use 

of this “tiered” testing framework saves valuable time and resources by organizing the studies in 

a cohesive and coherent manner and eliminating unnecessary lines of investigation. Lower tier, 

high dose screening studies also allow tighter control over experimental variables and exposure 

conditions, resulting in a greater ability to produce statistically reliable results at relatively low 

cost3

. 

3

 Non-target invertebrate hazard tests often are conducted at exposure concentrations several times higher than the 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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Tiered tests are designed to first represent unrealistic worst case scenarios and ONLY progress to 

real world field scenarios if the earlier tiered tests fail to indicate adequate certainty of acceptable 

risk. Screening (Tier I) non-target organism hazard tests are conducted at exposure 

concentrations several times higher than the highest concentrations expected to occur under 

realistic field exposure scenarios. This has allowed an endpoint of 50% mortality to be used as a 

trigger for additional higher-tier testing. Less than 50% mortality under these conditions of 

extreme exposure suggest that population effects are likely to be negligible given realistic field 

exposure scenarios. 

EPA uses a tiered (Tiers I-IV) testing system to assess the toxicity of a PIP to representative nontarget organisms that could be exposed to the toxin in the field environment. Tier I high dose 

studies reflect a screening approach to testing designed to maximize any toxic effects of the test 

substance on the test (non-target) organism. The screening tests evaluate single species in a 

laboratory setting with mortality as the end point. Tiers II – IV generally encompass definitive 

hazard level determinations, longer term greenhouse or field testing, and are implemented when 

unacceptable effects are seen at the Tier I screening level. 

Testing methods that utilize the tiered approach were last published by EPA as Harmonized 

OPPTS Testing Guidelines, Series 850 and 885 (EPA 712-C-96-280, February 1996)4

. These 

guidelines, as defined in 40 CFR 152.20, apply to microbes and microbial toxins when used as 

pesticides, including those that are naturally occurring, and those that are strain-improved, either 

by natural selection or by deliberate genetic manipulation. EPA has determined that it is 

appropriate to utilize these testing guidelines in the context of PIPs. 

The Tier I screening maximum hazard dose (MHD) approach to environmental hazard 

assessment is based on some factor (whenever possible >10) times the maximum amount of 

active ingredient expected to be available to terrestrial and aquatic non-target organisms in the 

environment (EEC)5

. Tier I tests serve to identify potential hazards and are conducted in the 

laboratory at high dose levels that increase the statistical power to test the hypotheses. Elevated 

test doses, therefore, add certainty to the assessment, and such tests can be well standardized. 

The Guidelines call for initial screening testing of a single group or several groups of test 

animals at the maximum hazard dose level. The Guidelines call for testing of one treatment 

group of at least 30 animals or three groups of 10 test animals at the screening test concentration. 

The Guidelines further state that the duration of all Tier I tests should be approximately 30 days. 

maximum concentrations expected to occur under realistic exposure scenarios. This has customarily allowed an endpoint of 50% 

mortality to be used as a trigger for additional higher-tier testing. Lower levels of mortality under these conditions of extreme 

exposure suggest that population effects are likely to be negligible given realistic exposure scenarios. Thus, it follows that the 

observed proportion of responding individuals can be compared to a 50% effect to determine if the observed proportion is 

significantly lower than 50%. For example, using a binomial approach, a sample size of 30 individuals is sufficient to allow a

treatment effect of 30% to be differentiated from a 50% effect with 95% confidence using a one-sided Z test. A one-sided test is 

appropriate because only effects of less than 50% indicate that further experiments are not needed to evaluate risk. 4

 http://www.epa.gov/opptsfrs/publications/OPPTS_Harmonized/885_Microbial_Pesticide_Test_Guidelines/Series/ 5

 The dose margin can be less than 10x where uncertainty in the system is low or where high concentrations of test 

material are not possible to achieve due to test organism feeding habits or other factors. High dose testing also may not be 

necessary where many species are tested or tests are very sensitive, although the test concentration used must exceed 1X EEC. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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Some test species, notably non-target insects, may be difficult to culture and the suggested test 

duration has been adjusted accordingly. Control and treated insects should be observed for at 

least 30 days, or in cases where an insect species cannot be cultured for 30 days, until negative 

control mortality rises above 20 percent. 

Failing the Tier I (10 X EEC) screening does not necessarily indicate the presence of an 

unacceptable risk in the field but it triggers the need for additional testing.6

 A less than 50% 

mortality effect at the MHD is taken to indicate minimal risk. Greater than 50% mortality does 

not necessarily indicate the existence of unacceptable risk in the field, but it does trigger the need 

to collect additional dose-response information and a refinement of the exposure estimation 

before deciding if the risk is acceptable or unacceptable. Where potential hazards are detected in 

Tier I testing (i.e. mortality is greater than 50%), additional information at lower test doses is 

required which can serve to confirm whether any effect might still be detected at more realistic 

field [1X EEC] concentrations and routes of exposure7. 

When screening tests indicate a need for additional data, the OPPTS Harmonized Guidelines call 

for testing at incrementally lower doses in order to establish a definitive LD50 and to quantify the 

hazard. In the definitive testing, the number of doses and test organisms evaluated must be 

sufficient to determine an LD50 value and, when necessary, the Lowest Observed Effect 

Concentration (LOEC), No Observed Adverse Effect Level (NOAEL) , or reproductive and 

behavioral effects such as feeding inhibition, weight loss, etc. In the final analysis, a risk 

assessment is made by comparing the NOAEL to the EEC; when the EEC is lower than the 

NOAEL, a no risk conclusion is made. These tests offer greater environmental realism, but they 

may have lower statistical power. Appropriate statistical methods, and appropriate statistical 

power, must be employed to evaluate the data from the definitive tests. Higher levels of 

replication, test species numbers or repetition are needed to enhance statistical power in these 

circumstances. 

Data that show less than 50 % mortality at the maximum hazard dosage level – (i.e., LC50, ED50, 

or LD50 >10 X EEC) is sufficient to evaluate adverse effects, making lower field exposure dose 

definitive testing unnecessary. It is also notable that the recommended >10X EEC maximum 

hazard dose level is a highly conservative factor. The published EPA Level of Concern [LOC] is 

50% mortality at 5X EEC 8

. 

6

 It is notable that that the 10 X EEC MHD testing approach is not equivalent to what is commonly known as “testing at 

a 10X SAFETY FACTOR” where any adverse effect is considered significant. Tier I screen testing is not ‘safety factor testing’. 

In a “10X safety factor” test any adverse effect noted is a “level of concern”, whereas in the EPA environmental risk assessment 

scenario any adverse effect is viewed as a concern only at 1X the field exposure. 7

 The 1X EEC test dose is based on plant tissue content and is considered a high worst case dose (sometimes referred to 

as HEEC). This 1X EEC is still much greater than any amount which any given non-target organism may be ingesting in the field 

because most non-target organisms do not ingest plant tissue. 8

 Environmental Protection Agency (USEPA) (1998). “Guidelines for Ecological Risk Assessment.” EPA 630/R-95-

002F. Washington, DC, USA. [Federal Register, May 14, 1998. 63(93): 26846-26924.] The established peer and EPA Science 

Board reviewed guidance on screening test levels of concern is 50% mortality at 5X environmental concentration. The 

appropriate endpoints in high dose limit/screening testing are based on mortality of the treated, as compared to the untreated

(control) non-target organisms. A single group of 30 test animals may be tested at the maximum hazard dose.
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Validation: The tiered hazard assessment approach was developed for EPA by the American 

Institute of Biological Sciences (AIBS) and confirmed, in 1996, as an acceptable method of 

environmental hazard assessment by a FIFRA Scientific Advisory Panel (SAP) on microbial 

pesticides and microbial toxins. The December 9, 1999, SAP agreed that the Tiered approach 

was suitable for use with plant-incorporated protectants; however, this panel recommended that, 

for PIPs with insecticidal properties, additional testing of beneficial invertebrates closely related 

to target species and/or likely to be present in GM crop fields should be conducted. Testing of Bt

Cry proteins on species not closely related to the target insect pest was not recommended, 

although it is still performed to fulfill the published EPA non-target species data requirements. In 

October 2000, another SAP also recommended that field testing should be used to evaluate 

population-level effects on non-target organisms. The August 2002 SAP, and some public 

comments, generally agreed with this approach, with the additional recommendation that 

indicator organisms should be selected on the basis of potential for field exposure to the subject 

protein.9 

Chronic studies: Since delayed adverse effects and/or accumulation of toxins through the food 

chain are not expected to result from exposure to proteins, protein toxins are not routinely tested 

for chronic effects on non-target organisms. The 30 day test duration requirement does, 

however, amount to subchronic testing when performed at field exposure test doses. Proteins do 

not bioaccumulate. The biological nature of proteins makes them readily susceptible to 

metabolic, microbial, and abiotic degradation once they are ingested or excreted into the 

environment. Although there are reports that some proteins (Cry proteins) bind to soil particles, 

it has also been shown that these proteins are degraded rapidly by soil microbial flora upon 

elution from soil particles. 

Conclusion: The tiered approach to test guidelines ensures, to the greatest extent possible, that 

the Agency requires the minimum amount of data needed to make scientifically sound regulatory 

decisions. EPA believes that maximum hazard dose Tier I screening testing presents a 

reasonable approach for evaluating hazards related to the use of biological pesticides and for 

identifying negative results with a high degree of confidence. The Agency expects that Tier 1 

testing for short-term hazard assessment will be sufficient for most studies submitted in support 

of PIP registrations. If long range adverse effects must be ascertained, then higher-tier longerterm field testing will be required. The Agency has been frequently asking the registrants to 

9

 EPA-SAP. February 4, 2000. Characterization and non-target organism data requirements for protein plant-pesticides. 

SAP report No. 99-06A for FIFRA Scientific Advisory Panel Meeting held December 8, 1999, held at the Sheraton Crystal City 

Hotel, Arlington, VA. 

EPA-SAP. November 6, 2002. Corn rootworm plant-incorporated protectant insect resistance management and nontarget insect issues. Transmittal of meeting minutes of the FIFRA Scientific Advisory Panel Meeting held August 27-29 at the 

Marriott Crystal City Hotel, Arlington, VA. 

EPA-SAP. March 12, 2001. Bt plant-pesticides risk benefit assessments. SAP report No. 2000-07 for FIFRA Scientific 

Advisory Panel Meeting held October 18-20, 2000 at the Marriott Crystal City Hotel, Arlington, VA. 

EPA-SAP. August 19, 2004. Product characterization, human health risk, ecological risk, and insect resistance 

management for Bacillus thuringiensis (Bt) cotton products. Transmittal of meeting minutes of the FIFRA Scientific Advisory 

Panel Meeting held June 8-10 at the Holiday Inn Ballston, Arlington, VA. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

36

 

conduct post-registration long term invertebrate population/community and Cry protein 

accumulation in soils studies as a condition of registration. As noted above, the October 2000 

SAP and the National Academy of Sciences10 (NAS 2000) recommended testing non-target 

organisms directly in the field. This approach, with an emphasis on testing invertebrates found 

in crop fields, was also recommended by the August 2002 SAP and was supported by several 

public comments. The issue of long range effects of cultivation of currently registered Cry 

proteins on the invertebrate community structure in Bt crop fields has since been adequately 

addressed by a meta analysis of field studies performed during the last 10 years. No unexpected 

adverse effects on invertebrate community structure were reported.11 The meta analysis of short 

term and long term field study effects on invertebrate populations in Bt corn and cotton fields 

indicate that no unreasonable adverse effects are taking place as a result of wide scale Bt crop 

cultivation. The Agency is in agreement with these conclusions. Slight reductions in some 

invertebrate predator populations are an inevitable result of all pest management practices which 

result in reductions in the abundance of the pests as prey. Based on these considerations, 

regulatory testing of the specialist predators and parasitoids of target pests may eventually be 

considered unnecessary. 

 

 2. Environmental Exposure Assessment 

The EPA risk assessment is centered only on adverse effects at the field exposure rates (1X 

EEC), and not on adverse effects at greater concentrations. The dose margin can be less than 

10x where uncertainty in the system is low or where high concentrations of test material are not 

possible to achieve due to test organism feeding habits. High dose testing also may not be 

necessary where many species are tested or tests are very sensitive, although the concentration 

used must exceed 1X EEC. It is important to note that Tier I screen testing is not “safety factor 

testing.” In a traditional “10X safety factor” test any adverse effect noted is a “level of concern”, 

whereas in the EPA environmental risk assessment scenario any adverse effect is viewed as a 

concern only at 1X the field exposure. 

For the purposes of the nontarget organism (NTO) studies submitted in support of the MON 

89034 registration, test material dose levels were based on the estimated concentration of 

Cry1A.105 and/or Cry2Ab2 protein expressed in the tissue(s) that NTO would most likely be 

exposed to in the environment (see Edelstein, 2007 for protein expression levels). Whenever 

possible, a targeted margin of exposure (MOE) of greater than 10X the maximum environmental 

exposure was used in the tests. The primary route of Cry1A.105 and Cry2Ab2 protein exposure 

for honeybee, ladybird beetle, parasitic wasp, and minute pirate bug is corn pollen. 

Consequently, test material dose levels were based on the maximum level of measured protein 

10 Environmental Effects of Transgenic Plants: The Scope and Adequacy of Regulation is available from the National 

Academy Press, 2101 Constitution Avenue, N.W., Lockbox 285, Washington, DC 20055; (800) 624-6242 or (202) 334-3313 (in 

the Washington metropolitan area); http://www.nap.edu. 11 Marvier, M., McCreedy, C., Regetz, J. & Kareiva, P. A meta-analysis of effects of Bt cotton and maize on nontarget 

invertebrates. Science 316, 1475–1477 (2007). 

Sanvido,O., Romeis, J., Bigler, F. (2007). Ecological Impacts of Genetically Modified Crops: Ten Years of Field 

Research and Commercial Cultivation. Adv Biochem Engin/Biotechnol 107: 235–278 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

37

expression in pollen (8.8 ug/g fwt for Cry1A.105 and 0.47 ug/g fwt for Cry2Ab2). The principal 

route of Cry1A.105 and Cry2Ab2 protein exposure for soil-dwelling organisms, such as 

Collembola and earthworms, is assumed to be from decomposing plant tissue and plant exudates 

in soil. Consequently, the test material dose levels were based on the maximum level of 

estimated protein concentration in the soil environment. 

 

3. Non-Target Wildlife Hazard Assessment for MON 89034 corn 

 

Two separate SAP reports (October 2000 and August 2002) recommended that non-target testing 

of Bt Cry proteins should focus on invertebrate species exposed to the crop being registered. 

Following the SAP recommendations, EPA determined that non-target organisms with the 

greatest exposure potential to Cry protein in transgenic corn fields are beneficial insects, which 

feed on corn pollen and nectar, and soil invertebrates, particularly Coleopteran species. 

Therefore, maximum hazard dose toxicity testing on representative beneficial organisms from 

several taxa was performed in support of this Section 3 FIFRA registration. The toxicity of the 

Cry1A.105 and Cry2Ab2 proteins has been evaluated on several species of invertebrates 

including the lady beetle, minute pirate bug, parasitic hymenoptera, collembola, daphnia, honey 

bee, and earthworm. Developmental observations were also made in the lady beetle, minute 

pirate bug and honeybee studies. Observations of possible reproductive effects were made in the 

collembola studies. 

Although the Cry1A.105 and Cry2Ab2 proteins in MON 89034 are known to be very host 

specific, conferring toxic effects on ECB, CEW, FAW, BCW and closely related species, and 

despite the October 2000 and August 2002 SAP’s recommendations against testing of non-target 

species not related to susceptible target pests, EPA has done a risk assessment on a range of nontarget wildlife to comply with the Agency’s published non-target data requirements (in the 

absence of PIP-specific risk assessment guidance, EPA requires applicants for PIP registrations 

to meet the 40 CFR Part 158 data requirements for microbial toxins). These requirements 

include birds, mammals, plants and aquatic species. In addition, an earthworm study was 

voluntarily submitted to the Agency to ascertain the potential effects of the Cry1A.105 and 

Cry2Ab2 proteins on beneficial decomposer species. 

Test substances used for studies submitted in support of the event MON 89034 registration 

included bacterially produced purified Cry1A.105 and Cry2Ab2 proteins and MON 89034 corn 

grain. The October 2000 SAP recommended that while actual plant material is the preferred test 

material, bacterially-derived protein is also a valid test substance, particularly in scenarios where 

test animals do not normally consume corn plant tissue and where large amounts of Cry protein 

(Cry protein concentrations that exceed levels present in plant tissue) are needed for maximum 

hazard dose testing. An insect feeding study, which compared the relative potency of plant 

produced Cry1A.105 and Cry2Ab2 proteins to the microbe produced proteins, indicated that 

plant produced protein was similar in toxicity to the microbe produced protein (Edelstein Memo, 

November 7, 2007). 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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Potential Interaction between the Cry1A.105 and Cry2Ab2 proteins was addressed in a 

memorandum for the MON 89034 Experimental Use Permit accompanying the Agency’s 

review of “Evaluation of the Potential for Interactions Between the Bacillus thuringiensis

Proteins Cry1A.105 and Cry2Ab2,” (Hunter, M., July 6, 2006). The purpose of this study was 

to characterize the potential for interaction between the lepidopteran-active proteins Cry1A.105 

and Cry2Ab2. The study provides evidence that the proteins do not interact in an antagonistic, 

or synergistic manner and that there will not be any unexpected interaction with regard to target 

and non-target insects. New data on the potential interaction between combined Cry1A.105, 

Cry2Ab2 with the Cry3Bb1 protein was submitted. The results from the study demonstrated 

that combined Cry1A.105 and Cry2Ab2 activity was not affected by the Cry3Bb1 protein and 

that Cry3Bb1 activity was not affected by combined Cry1A.105 and Cry2Ab2 activity (MRID 

469513-05 & 469513-06). 

The results of ecological effects studies submitted in support of the MON 89034 Section 3 

FIFRA registration are summarized in Table 5 and presented in a more descriptive format in 

subsequent sections of this risk assessment document. Full reviews of each study can be found 

in the individual Data Evaluation Reports (DERs/MRID#s) and accompanying memos. 

Table 5. Summary of environmental effects studies and waiver justifications submitted to 

comply with data requirements published in 40 CFR § 158.2150(d). 

Data 

Requirement 

Guideline Classification Test 

Substance 

Results Summary MRID #

Avian oral 885.4050* 

154-16** 

Acceptable MON 89034 

corn grain†

A 42-day dietary study showed that 

Event MON 89034 did not 

adversely affect broiler chickens. 

469514-12 

Avian injection 885.4100 

154-17 

Acceptable waiver 

rationale 

N/A N/A N/A 

Avian acute oral 850.2100 Acceptable MON 89034 

corn grain 

An eight-day dietary study showed 

that the LC50 for MON 89034 is 

>500,000 ppm in the diet northern 

bobwhite quail. 

469514-27 

Wild mammal 885.4150 

154-18 

Acceptable waiver 

rationale 

N/A N/A N/A 

Freshwater fish 885.4200 

154-19 

Acceptable waiver 

rationale 

N/A Freshwater fish studies were not 

required because of the low 

potential that fish will be exposed to 

high levels of the Cry1A.105 and 

Cry2Ab2 proteins 

N/A 

Freshwater 

aquatic 

invertebrate 

Daphnia magna 

885.4240 

154-20 

Unacceptable 

[The 885 Series 

Guidelines call for 

a 21 day study. 

The submitted 48 

hour acute study is 

inadequate.] 

MON 89034 

corn 

Pollen 

A 48-hour static renewal limit 

bioassay resulted in 17% mortality 

compared with 0% mortality in the 

control groups (120 mg/L). A 48-

hour static renewal dose-response 

bioassay was conducted and no 

mortality or adverse effects were 

observed at any concentration (6.3-

120 mg/L). The acute EC50 was 

estimated to be >120 mg/L and the 

NOEC was 100 mg/L. 

469514-17 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

39

Guideline Classification Test 

Substance 

Data Results Summary MRID #

Requirement 

Estuarine and 

marine animal 

885.4280 

154-21 

Acceptable waiver 

rationale 

N/A N/A N/A 

Non-target plant 885.4300 

154-22 

Acceptable waiver 

rationale 

N/A N/A N/A 

Non-target 

insect testing, 

minute 

pirate/insidious 

flower bug 

Orius insidiosus

885.4340 

154-23 

Acceptable Cry2Ab2 

protein 

(Lot No. 

20-100071) 

Orius nymphs were fed a pollen diet 

containing 100 μg Cry2Ab2 

protein/diet for 14 days. No adverse 

effects were observed. 

469514-24 

Non-target 

insect testing, 

minute 

pirate/insidious 

flower bug 

Orius insidiosus

885.4340 

154-23 

Acceptable Cry1A.105 

protein 

(Lot No. 

 20-100073) 

Orius nymphs were fed a diet 

containing 30 to 240 μg 

Cry1A.105/g diet for 14 days. In an 

initial maximum dose test (240 μg) 

the survival rate was 47% compared 

to 88% in the control groups. In the 

three subsequent dose-response 

tests, the mean survival rate of the 

240 μg group was 55% compared to 

91% and 89% in the control groups. 

No statistically significant effects on 

survival or development were seen 

at concentrations less than or equal 

to 120 μg Cry1A.105/g diet. 

469514-23 

Non-target 

insect testing, 

parasitic wasp, 

Ichneumon 

promissorius

885.4340 

154-23 

Acceptable Cry2Ab2 

protein 

(Lot No. 

20-100071) 

Adult female wasps were fed a 

sucrose solution containing 100 μg 

Cry2Ab2 protein/mL for 21 days. 

Mortality in the Cry2Ab2 group was 

3% and the LC50 was determined to 

be >100 μg/L. 

469514-26 

Non-target 

insect testing, 

parasitic wasp 

Ichneumon 

promissorius

885.4340 

154-23 

Acceptable Cry1A.105 

protein 

(Lot No. 

20-100073) 

Adult female wasps were fed a 

sucrose solution containing 240 μg 

Cry1A.105 protein/mL for 21 days. 

Mortality in the Cry1A.105 group 

was 7% and the LC50 was 

determined to be >240 μg/L. 

469514-25 

Non-target 

insect testing, 

ladybird beetle 

Coleomegilla 

maculata

885.4340 

154-23 

Acceptable Cry2Ab2 

protein 

(Lot No. 

 20-100071) 

C. maculata larvae were fed a diet 

containing 120 μg Cry2Ab2 

protein/g diet for 17-20 days. No 

statistically significant difference in 

survival or development to adult 

was found between the test and 

control groups. A slight (~5%) 

statistical decrease in mean adult 

body weight was found between the 

test and buffer control groups; 

however, this difference was not 

observed between the test and assay 

control group. 

469514-22 

Non-target 

insect testing, 

ladybird beetle 

Coleomegilla 

maculata

885.4340 

154-23 

Acceptable Cry1A.105 

protein 

(Lot No. 

20-100073) 

Ladybird beetle larvae were fed a 

diet containing 240 μg Cry1A.105 

protein/g diet for 14 days. No 

statistically significant differences 

in survival, development, or adult 

469514-21 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

40

Guideline Classification Test 

Substance 

Data Results Summary MRID #

Requirement 

beetle weight were found between 

the test and control groups. 

Non-target 

insect testing, 

collembolan 

Folsomia 

candida

885.4340 

154-23 

Acceptable MON 89034 

Leaf Tissue 

(80 μg 

Cry1A.105 

and 70 μg 

Cry2Ab2/g 

diet) 

Collembola were fed a diet 

containing 50% Brewer’s yeast and 

50% lyophilized leaf tissue for 28 

days. No statistically significant 

effects on survival or reproduction 

were found among the test and 

negative control groups. 

469514-16 

Honeybee 

testing, Adult 

Honeybee, Apis 

mellifera

885.4380 

154-24 

Acceptable Cry1A.105 

protein 

(Lot No. 

 20-100073) 

Adult honeybees were fed a 30% 

sucrose solution containing 550 μg 

Cry1A.105 protein/mL for 19 days. 

No statistically significant 

differences in mortality were 

observed between the test group and 

negative controls. The NOEC was 

determined to be at least 550 μg 

Cry1A.105 protein/mL. 

469514-20 

Honeybee 

testing, 

Honeybee 

larvae, Apis 

mellifera

885.4380 

154-24 

Acceptable Cry1A.105 

protein 

(Lot No. 

20-100073) 

Two-to-three day old honeybee 

larvae in brood frames were 

administered a single 10 μL dose of 

Cry1A.105 protein per brood cell 

(equivalent to 12 μg total 

protein/cell). On day 18 after dosing 

mean survival of the test group was 

95%. The NOEC was determined 

to be at least 12 μg Cry1A.105 

protein per brood cell 

469514-20 

Earthworm 

subchronic 

toxicity, Eisenia 

fetida

850.620 Acceptable Cry1A.105 

protein 

(Lot No. 

20-100073) 

Adult earthworms were exposed to 

artificial soil containing 178 mg 

Cry1A.105 protein/kg dry soil for 

14 days. No mortality was observed 

in the test group. The LC50 was 

determined to be >178 mg 

Cry1A.105/kg dry soil and the 

NOEC was 178 mg Cry1A.105 

mg/kg dry soil. 

469514-18 

Soil fate 885.5200 Acceptable Purified 

Cry1A.105 

(Lot No. 

20-100073) 

and 

Cry2Ab2 

protein 

(Lot No. 

 20-100071) 

Results of this degradation study 

indicate that Cry1A.105 and 

Cry2Ab2 proteins do not persist in 

soil beyond approximately three 

weeks. 

469514-28 

Note: Earthworm and honey bee studies for Cry2Ab2 protein were submitted and reviewed with previously registered products. 

The interaction study between Cry1A.105 and Cry2Ab2 was reviewed for the MON 89034 Experimental Use Permit 

*OPPTS Microbial pesticide test guidelines

**Microbial pesticide test guidelines identified in the 40 CFR data tables. 

† Cry1A.105 and Cry2Ab2 are the active ingredients (a.i.) in MON 89034 corn. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

41

a) Non-target Wildlife Study Summaries 

 

 i. Avian species

Published data and studies on file at EPA show that consumption of Bt corn has no measurable 

deleterious effects on avian species. To comply with published data requirements, the following 

studies were submitted to EPA in support of the MON 89034 product registration. These studies 

were GLP compliant and, when considered together, meet EPA data requirements for avian 

species. 

(a) Broiler (MRID 469514-12) 

For the first 42 days of life, commercial broiler chickens (Gallus domesticus) were fed a corn 

and soybean diet that contained up to 59% ground corn grain. Treatments consisted of soybean 

meal with MON 89034, a similar isoline (negative control), or one of four different commercial 

hybrid corn varieties. At test end, chickens were processed in order to obtain performance and 

carcass yield data. Breast and thigh meat were also analyzed for moisture, protein, and fat 

content. Among treatments, there were no biologically significant differences in broiler 

performance, carcass, or meat quality. 

 (b) Northern Bobwhite Quail (MRID 469514-27) 

In this eight-day dietary study, 10-day-old northern bobwhites (Colinus virginianus) were fed a 

corn and game bird ration containing 50% ground corn grain. Treatments consisted of game bird 

ration with MON 89034, a similar isoline (negative control), or one of three different commercial 

hybrid corn varieties. At test end, no mortality was seen in the MON 89034 treatment group, all 

birds appeared normal for test duration, and feed consumption was comparable to that of the 

control group. The dietary LC50 of MON 89034 corn grain was determined to be >500,000 ppm 

in the diet. 

 ii. Wild mammalian species 

Mammalian wildlife exposure to Cry1A.105 and Cry2Ab2 proteins is considered likely; 

however, mammalian toxicology information gathered to date on Bt Cry proteins does not show 

a hazard to wild mammals. In addition, acute oral toxicity studies submitted to EPA in support 

of the MON 89034 registration indicated that no significant toxicity was seen when rodents were 

exposed to Cry1A.105 or Cry2Ab2 at the maximum hazard dose level. Therefore, no hazard to 

mammalian wildlife is anticipated and data on wild mammal testing is not required for this 

registration. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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42

iii. Aquatic species 

There is no evidence for sensitivity of aquatic species to anti-coleopteran Cry proteins. A 

published laboratory study with lepidopteran-active Cry proteins has revealed that the leaf 

shredding (caddis fly) trichopteran, Lepidostoma liba, had 50% lower growth rate when fed Bt

corn litter (Rosi-Marshall, et al. 2007). Two previous field study reports by the same authors did 

not find adverse effects on head stream invertebrates. The Agency’s position on this matter is 

that until Tier III and Tier IV field studies are performed, there is not enough information to 

assert that sufficient corn plant litter enters streams to cause unreasonable adverse effects on 

stream invertebrate populations or communities (See Section D. 1. above - Tiered Hazard and 

Risk Assessment Process). Two years ago the Iowa State University and the University of 

Maryland received Research grants to study the effects of Bt corn cultivation on streams and to 

develop methods for aquatic hazard assessment. The results of these studies are pending. When 

the study reports are reviewed the Agency will respond with action commensurate with the 

outcome of the studies. 

The Agency’s current position is that there is no evidence to conclude that there is sufficient 

aquatic exposure to Cry proteins in corn plant litter to result in adverse effects on stream 

invertebrate populations or communities. Aquatic animal exposure to Bt crops is extremely 

small. 

 (a) Freshwater fish-Waiver granted 

Freshwater fish studies were not required for this product, because of the low potential that 

aquatic systems will be exposed to the Cry1A.105 and Cry2Ab2 proteins produced in MON 

89034 corn plant tissues. 

 (b) Freshwater aquatic invertebrates (MRID 469514-17) 

The objective of this study was to determine the potential for acute effects to the aquatic 

organism, Daphnia magna, during a static renewal exposure to MON 89034 corn pollen. The 

test was initially conducted as a limit test using one test concentration. Slight effects were noted 

at the limit concentration. In response, a dose-response test was conducted. The test substance, 

MON 89034 pollen expressing Cry1A.105 and Cry2Ab2 proteins, was evaluated for potential 

adverse effects to neonate Daphnia. Test organisms were < 24 hours old at test initiation and 

came from in-house cultures at the test facility. 

Limit Test

In the initial test, daphnids were exposed to a single nominal test concentration of 120 mg 

pollen/L for 48 hours with renewal of the test solution at approximately 24 hours. Two control 

groups were included: a group in well water exposed to pollen (120 mg/L) from conventional 

corn with a genetic background similar to MON 89034, and an assay control group exposed to 

well water only. Each treatment was replicated three times and each replicate contained 10 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

43

neonate daphnids. Test chambers consisted of 600-mL glass beakers containing 300 mL of the 

appropriate treatment solution. Observations of mortality, immobility and other clinical signs 

were made at approximately 3.5, 24 and 48 hours after test initiation. At test termination there 

was 17% immobility in the 120 mg/L treatment group, with two daphnids exhibiting lethargy. 

All daphnids in the assay control group and 120 mg pollen/L control group appeared normal 

throughout the testing period. 

Dose-Response Test

In the dose-response test, daphnids were exposed to six concentrations of MON 89034 pollen for 

48 hours. The concentrations tested were 6.3, 13, 25, 50, 100, and 120 mg pollen/L. Two 

control groups were included: a group in well water exposed to pollen (120 mg pollen/L) from 

conventional corn with a genetic background similar to MON 89034, and an assay control group 

exposed to well water only. The test and control solutions were renewed at approximately 24 

hours. Each treatment was replicated two times and each replicate contained 10 neonate 

daphnids. Test chambers consisted of 600-mL glass beakers containing 300 mL of the 

appropriate treatment solution. Observations of mortality, immobility and other clinical signs 

were made at approximately 5, 24, and 48 hours after test initiation. The NOEC was estimated 

by visual interpretation of the mortality, immobility and observation data. At test termination 

there were no mortalities, immobile daphnids or signs of toxicity noted in any control or test 

substance group during the 48 hour exposure period. 

Conclusions: Based on the results of the dose-response test, the 48-hour EC50 was estimated to 

be greater than 120 mg MON 89034 pollen/L. Based on the results of both studies, the 48-hour 

NOEC was 100 mg MON 89034 pollen/L. This study is unacceptable because it is an 850 Series 

OPPTS Guideline study. The 48 hour test duration is not sufficient to show mortality for Bt

toxins. The 48 hours test duration is not considered to be sufficient duration to assess the 

potential for adverse effects to non-target organisms. Consistent with the 885 Series OPPTS 

Guidelines, a 7 to 14 day Daphnia study is necessary. The study may be submitted as a condition 

of registration. Alternatively, a dietary study of the effects on an aquatic invertebrate, 

representing the functional group of a leaf shredder in headwater streams, may be performed and 

submitted in lieu of the Daphnia study. 

 (c) Estuarine and marine animals-Waiver granted 

Estuarine and marine animal studies were not required for this product, because of the low 

probability that estuarine or marine systems will be exposed to the Cry1A.105 and Cry2Ab2 

proteins produced in MON 89034 corn plant tissues. 

 

iv. Terrestrial and aquatic plant species-Waiver granted

Plant toxicity studies were not required for this product because the active ingredient is an insect 

toxin (Bt endotoxin) that has never shown any toxicity to plants. 

 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

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 v. Terrestrial Invertebrate species 

The Cry1A.105 and Cry2Ab2 proteins are meant to target species within the order Lepidoptera 

(moths and butterflies). Bt toxins are known to have a limited host range, however, to address 

any unforeseen change in activity spectrum and to fulfill the published registration data 

requirements EPA requires that test species used for non-target insect evaluations should include 

several species that are not related to the target pests. Earthworm studies are also recommended. 

 (a) Ladybird beetle 

 

MRID 469514-21

The purpose of this study was to determine the potential dietary effects of the Cry1A.105 protein 

on the mortality and development of the ladybird beetle, Coleomegilla maculata. The test 

substance, Cry1A.105 protein, was produced by a recombinant E. coli fermentation system. The 

test substance was incorporated at 240 μg Cry1A.105 protein/g of diet. The diet consisted of an 

artificial agar-based diet. Three control treatments were included in the experiment. The buffer 

control contained 25 mM CAPS buffer, which was the buffer used for storage of the test 

material. The assay control (purified water) was used to generate a diet-only treatment and a 

positive control treatment was also tested, containing potassium arsenate. Ladybird beetle larvae 

were less than 48 hours old at test initiation. The larvae were contained in individual test arenas 

(inverted 60 x 15 mm Petri dishes) and were allowed to feed ad libitum on the appropriate test 

diet. Each treatment was replicated six times and each replicate contained 15 or 16 larvae. All 

six replicates met the acceptance criteria of less than or equal to 20% mortality; the mortality of 

larvae ranged from 0 to 18.8% in the assay control treatment groups. The diet treatments were 

replaced with fresh diet approximately every 48 to 72 hours. The larvae were monitored every 

24 to 72 hours for survival and development to the adult stage. Adults were weighed within 30 

hours of eclosion and each adult was dissected and sexed. Any abnormal behavior or 

development was noted during feedings and observational evaluations. The study duration 

ranged from 17 to 20 days depending on adult emergence. Samples were taken to test the 

biological activity, homogeneity, and stability of the Cry1A.105 protein. Results showed that 

there were no differences in the mean survival percentage of C. maculata between the 

Cry1A.105 protein, buffer control, and assay control treatments (88.5%, 87.5%, 91.6%). The 

survival rate was 2.08% for the positive control treatment. There were no significant differences 

in the mean percent of C. maculata that developed to adults when comparing the Cry1A.105, 

buffer control, and assay control treatments (88.5%, 85.4%, 90.6%). None of the larvae 

developed to adults in the positive control treatment. Further, there were no significant 

differences in the mean weight of C. maculata adults between the Cry1A.105, buffer control, and 

assay control. No significant interaction was found between insect sex and treatment. In the 

bioactivity confirmation assay, results indicated equivalent biological activity among the 

Cry1A.105 used in the test diets and the reference standard. In addition, the homogeneity and 

stability study confirmed that Cry1A.105 was homogeneous in the test substance diet and was 

stable under the storage conditions employed in the study. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

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Conclusions: This study is acceptable. The results indicate that Cry1A.105 protein had no 

adverse effect on the survival, development, and growth of the ladybird beetles at a dietary 

concentration of 240 μg/g of diet. 

MRID 469514-22 

The objective of this study was to determine the potential dietary effects of Cry2Ab2 protein on 

the mortality and development of the ladybird beetle, Coleomegilla maculata. The test 

substance, Cry2Ab2, was produced by recombinant E. coli fermentation system. The endpoints 

evaluated were survival and development through 20 days (some replicates were completed 

before 20 days if all insects had developed to adults). The Cry2Ab2 protein was incorporated in 

to an agar-based artificial diet at a concentration of 120 μg Cry2Ab2/g diet. Three control 

treatments were included in the study: 1) buffer control, 2) assay control (purified water), and 3) 

positive control (potassium arsenate). The ladybird beetle larvae were less than 48 hours old 

when testing began and the larvae were allowed to feed ad libitum. The diet treatments were 

replaced approximately every 48 to 72 hours. Each treatment was replicated six times and each 

replicate included 14 to 16 ladybird beetle larvae. Each larva was contained in its own test arena 

which consisted of an inverted 60 x 15 mm Petri dish. The larvae were monitored every 24 to 72 

hours for survival and development to the adult stage. Adults were weighed within 30 hours of 

eclosion and adults were sexed. The biological activity, homogeneity, and stability of the 

Cry2Ab2 protein in the diet were confirmed in a separate bioassay using Helicoverpa zea. The 

mean survival for C. maculata was 94.7% for the Cry2Ab2 treatment, 88.8% for the buffer 

control treatment, 91.6% for the assay control, and 2.08% for the positive control. The mean 

percent of larvae that developed to adults was 92.6% in the Cry2Ab2 treatment, 85.3% for the 

buffer control treatment, and 90.6% for the assay control. None of the larvae developed to adults 

in the positive control treatment. The mean adult weights for the test material and groups were 

about 5% lower buffer control than those of the assay control group, which was a statistically 

significant difference. However, there was no significant difference in adult weight of the test 

material and buffer control groups. 

Conclusions: This study is acceptable. No adverse effects were seen in C. maculata fed 120 μg 

Cry2Ab2 protein/g diet. Although the mean adult weight of the Cry2Ab2 protein treatment 

group was statistically significantly lower than that of the assay control group, the difference was 

slight (~5%) and there was no significant difference between the weight of the Cry2Ab2 protein 

group and buffer control groups. 

 

 (b) Minute pirate bug 

MRID 469514-23 

The purpose of this study was to determine the potential dietary effects of Cry1A.105 protein on 

mortality and development of Orius insidiosus, the minute pirate bug or insidious flower bug. 

The Cry1A.105 protein (E. coli-produced) was incorporated into a pollen-based diet for 

treatment of the test group. Both a buffer control diet and an assay control diet (pollen diet only) 

were included in the study. A positive control group was fed a diet treated with potassium 
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arsenate. The initial test involved dosing the insects with a single maximum dose level (240 μg 

Cry1A.105/g diet) for 14 days, resulting in 47% survival. The assay and buffer control resulted 

in 88% survival. A total of 75 Orius were tested in each treatment. Based on the results of the 

maximum hazard dose assay, three 14-day dose-response tests were conducted with test 

substance exposure levels of 30, 60, 120 and 240 μg Cry1A.105/g diet. Again, a buffer control, 

assay control, and positive control were included in each of the three tests. Each exposure test 

was conducted independently at a different time using separate groups of Orius. During the test, 

Orius were supplied with a capsule (50 μL) of the appropriate diet and the capsules were 

replaced every other day. The test arenas consisted of 1-ounce plastic cups with plastic covers 

and each cup contained one Orius. For each dose-response exposure, 25 test arenas were 

included for each diet treatment. Observations and feeding behavior were recorded each feeding 

day for each test arena. Results of the first replicate resulted in percent survival in the 30, 60, 

120 and 240 μg Cry1A.105 protein/g diet was 88, 84, 88 and 56%, respectively. Percent survival 

in the assay, buffer, and positive control was 92, 88, and 36%, respectively. The percent of 

nymphs developing to adults for the 30, 60, 120 and 240 μg Cry1A.105 protein/g diet, assay 

control, buffer control and potassium arsenate control organisms was 92, 100, 96, 92, 96, 96 and 

40%, respectively. No statistically significant differences were detected between the 30, 60, 120 

and 240 μg Cry1A.105 protein/g diet. Percent survival in the second replicate in the 30, 60, 120 

and 240 μg Cry1A.105 protein/g diet was 88, 92, 92, and 52%, respectively. Percent survival in 

the assay and buffer controls was 88% and percent survival in the positive control was 32%. The 

percent of nymphs developing to adults for the 30, 60, 120 and 240 μg Cry1A.105 protein/g diet, 

assay control, buffer control and positive control organisms was 100, 92, 96, 92, 96, 92 and 96%, 

respectively. The mean number of days to development for all treatments was 6.0 days. Percent 

survival in the third replicate in the 30, 60, 120 and 240 μg Cry1A.105 protein/g diet treatments 

was 92, 80, 80, and 56%, respectively. Percent survival in the assay, buffer and positive controls 

was 92 and 28%, respectively. The percent of nymphs developing to adults for the 30, 60, 120 

and 240 μg Cry1A.105 protein/g diet, assay control, buffer control and positive control 

organisms was 100, 96, 100, 92, 100, 100 and 80% respectively. The mean number of days to 

development for all treatments was 6.0 days. Throughout the study samples of the test and 

control substances were taken to be used in a bioassay with Helicoverpa zea to confirm the 

presence of the test substance, homogeneity of the test substance in the diet, diet stability and 

bioactivity of the test material. The bioassay confirmed that the test substance was stable 

throughout the study, was biologically active at anticipated levels and was appropriately mixed 

in the test diet. 

Conclusions: This study is acceptable. Orius insidiosus were exposed for 14 days to a range of 

dietary concentrations of Cry1A.105. For the three dose-response replicates the mean survival 

for the 240 μg Cry1A.105/g diet treatments was 55%. Therefore, the LC50 value was empirically 

determined to be greater than 240 μg Cry1A.105/g diet. No adverse effects were observed at 

concentrations less than or equal to 120 μg Cry1A.105/g diet. 
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MRID 469514-24 

The purpose of this study was to determine the potential dietary effects of Cry2Ab2 protein on 

mortality and development of Orius insidiosus. The Cry2Ab2 protein was incorporated into a 

pollen-based diet at a concentration of 100 μg Cry2Ab2 protein/g diet. The protein was 

produced by a recombinant E. coli fermentation system. Both a buffer control treatment and an 

assay control (pollen-based diet only) were included in the study. In addition, a positive control 

was included which consisted of the pollen-diet treated with potassium arsenate. The duration of 

the experiment was 14 days which was long enough to observe the Orius develop from nymph to 

adult. The test insects were approximately 3 days old at test initiation. Each treatment contained 

seventy-five insects and each insect was contained in its own test arena. During the exposure 

period, one capsule of approximately 50 μL of the appropriate test diet was provided in each test 

arena on Day 0 and every other day thereafter for the duration of the test. Observations and 

feeding behavior were recorded each day for each test arena. The biological activity, 

homogeneity, and stability of Cry2Ab2 protein in the test diet were tested and confirmed in a 

separate bioassay using Helicoverpa zea. The percent survival of insects exposed to the 100 μg 

Cry2Ab2 protein/g diet treatment was 91%, which was similar to the percent survival of the 

insects in the buffer and assay control groups. The percent of nymphs developing to adults in the 

100 μg Cry2Ab2 protein/g diet treatment, assay control, buffer control and positive control was 

93, 95, 91, and 73%, respectively. The mean number of days to develop to adult for insects 

exposed to the 100 μg Cry2Ab2 protein/g diet, assay control, buffer control and positive control 

treatments was 6.1, 7.1, 8.0 and 6.0 days, respectively. 

Conclusions: This study is acceptable. No adverse effects were observed for Orius insidiosus at 

the concentration level of 100 μg Cry2Ab2 protein/g diet. Therefore, the LC50 is greater than 

100 μg Cry2Ab2 protein/g diet. 

 (c) Parasitic hymenoptera 

MRID 469514-25 

This study was conducted to evaluate the potential effects of acute exposure of Cry1A.105 

protein to the parasitic wasp, Ichneumon promissorius. The Cry1A.105 protein was 

administered to the wasps at a concentration of 240 μg/mL in a 30% sucrose solution. The 

protein was produced by a recombinant E. coli fermentation system. Three control treatments 

were included in the experiment: 1) buffer control, 2) assay control (sucrose solution only), and 

3) positive control (potassium arsenate). The positive control substance was tested at two 

concentrations (100 and 400 ppm). There were three replications per treatment and each 

replication contained 10 female wasps. The wasps were 3 to 6 days old at the time of test 

initiation. The test chambers were disposable 64 ounce containers. Observations of mortality 

and clinical signs were conducted once within the hour of test initiation and then continued daily 

until Day 21 of the test. Samples of the assay control, control substance and protein group diets 

were collected for analysis by bioassay to test for bioactivity and stability of the Cry1A.105 

protein. Mortality in the assay control, buffer control, and test material treatments was 10%, 8%, 

and 7% respectively. All surviving wasps in those groups appeared normal in appearance and 
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behavior. There was no statistically significant difference in mean mortality in the Cry1A.105 

treatment and buffer control treatments. The biological activity and stability of the Cry1A.105 

protein was confirmed in a seven-day bioassay using the corn earworm (Helicoverpa zea). 

Conclusions: This study is acceptable. The LC50 for Ichneumon promissorius was greater than 

240 μg Cry1A.105 protein/mL and the NOEC was at least 240 μg Cry1A.105 protein/mL. 

 MRID 469514-26 

A laboratory bioassay was conducted to evaluate the potential effects of acute exposure to 

Cry2Ab2 protein to the parasitic wasp Ichneumon promissorius. The Cry2Ab2 protein used was 

produced by an E. coli fermentation system. Wasps were exposed to the Cry2Ab2 at a 

concentration of 100 μg/L in a 30% sucrose solution. Three control groups were utilized, 

including: 1) buffer control, 2) negative assay control group (sucrose solution only) and 3) two 

positive controls using two concentrations of potassium arsenate. The test diets were prepared 

by diluting 60% (w:v) sucrose solution with equal amounts of solutions containing test and 

control substances in deionized water to obtain diets with approximately 30% sucrose. Test diet 

containing Cry2Ab2 protein was prepared weekly. The wasps were given fresh diet daily. Three 

replicate test chambers were used for each treatment and control group and 10 female wasps 

were contained in each test chamber. The test chambers consisted of disposable 64 oz. 

polypropylene containers. The wasps were approximately 3 to 6 days old at test initiation. 

Observations were made once during the hour of test initiation and once daily until Day 21 of the 

test. Samples of the assay control, control substance and protein group diets were collected for 

analysis by bioassay. The biological activity relative to a reference standard and stability of the 

Cry2Ab2 protein in the test diet was confirmed in a seven-day corn earworm (Helicoverpa zea) 

bioassay. At test termination (Day 21), mortality in the assay control, buffer substance, and test 

substance groups was 10%, 3%, and 3%, respectively. All surviving wasps were normal in 

appearance and behavior. No statistically significant differences in mean mortality were found 

between the Cry2Ab2 treatment group and the negative control group. 

Conclusions: This study is acceptable. The LC50 for Ichneumon promissorius was determined to 

be >100 μg Cry2Ab2 protein/mL and the NOEC was at least 100 μg Cry2Ab2 protein/mL. 

 (d) Collembola (MRID 469514-16) 

The objective of this study was to determine the potential effect of chronic exposure of 

lyophilized corn leaf tissue from MON 89034 maize on the survival and reproduction of 

Folsomia candida. The study sponsor verified the identity and the concentrations of Cry1A.105 

and Cry2Ab2 in the lyophilized leaf material. The concentration of Cry1A.105 was 160 μg/g 

lyophilized leaf dry weight and the concentration of Cry2Ab2 was 140 μg/g lyophilized leaf dry 

weight. The lyophilized test material was incorporated in to a diet containing 50% Brewer’s 

yeast and 50% test material (0.500g leaf tissue with 0.500 g yeast). Therefore, the test diet 

contained Cry1A.105 at a nominal concentration of 80 μg/g lyophilized leaf dry weight and 

Cry2Ab2 at a nominal concentration of 70 μg/g lyophilized leaf dry weight. A control diet was 
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prepared by mixing 50% control leaf tissue, by weight, with 50% Brewer’s yeast. An additional 

control treatment consisted of a test diet containing only Brewer’s yeast. Three positive control 

treatments were included. The three treatments included three treatments of thiodicarb, 

representing nominal concentrations of 1.0, 10 and 100 mg a.i./kg. Collembola were provided 

enough food such that an excess was always available. Each treatment contained four replicates 

and each replicate initially contained 10 juvenile Collembola (12 days old). Each replicate was 

contained in a glass jars containing a water saturated substrate consisting of plaster of Paris and 

charcoal at a ration of 8:1 by weight. The biological activity and concentration of Cry1A.105 

protein were confirmed in samples collected at the end of dosing. The bioassay confirmed that 

the test material was biologically active against CEW and the level of activity was not 

significantly different from that of the reference standard. Mortality and observations of 

sublethal effects of the surviving Collembola were recorded on day 28 (test termination). 

Collembola were removed from the test arenas and the number of adult and young Collembola 

were counted. Among the yeast-only diet control organisms, mean survival was 98% and mean 

reproduction was 170 offspring per arena. Mean survival of Collembola exposed to the control 

diet (control leaf tissue) and MON 89034 diet was 100 and 98%, respectively. The mean number 

of offspring produced in the control substance diet (control leaf tissue) and the MON 89034 diet 

was 260 and 257 offspring per arena, respectively. Mean survival in the positive control 

substance treatments 1.0, 10 and 100 mg thiodicarb/kg diet was 95, 63 and 35% respectively. 

The mean number of offspring produced in the 1.0, 10 and 100 mg thiodicarb/kg diet treatments 

was 136, 93, and 16 offspring per arena, respectively. Statistical analysis demonstrated no 

significant reduction in survival or reproduction among Collembola exposed to the MON 89034 

diet when compared to either negative control. 

Conclusions: The study is acceptable. The NOEC for Folsomia candida is at least 80 μg 

Cry1A.105 and 70 μg Cry2Ab2 per gram of diet. 

 (e) Honeybee

MRID 469514-19 

The objective of this study was to evaluate potential dietary effects of Cry1A.105 protein when 

administered to honeybee larvae (Apis mellifera). Honeybees were approximately 2 to 3 days 

old during the experiment. The test substance was Cry1A.105 protein produced by an E. coli

fermentation system. The protein was used to prepare a test solution at a concentration of 1200 

g/mL. This concentration is equivalent to 12 g total protein per cell. Additional treatment 

groups included an assay control, buffer control, and two reference substance concentrations 

(potassium arsenate at low and high doses). Each treatment included four replications of 20 bees 

for a total of 80 bees per treatment. The larvae were exposed to a single dose (10 mL) of the 

appropriate dosing solution at initiation and observed during larval and pupal development. 

Survival of larvae was assessed at study completion (Day 18) by observing adult emergence. 

The Cry1A.105 treatment group resulted in 95% survival. Survival in the assay control and 

buffer control was 92.5%. Adult emergence in the low and high dose potassium arsenate 

treatments was 26.5% and 5.0%, respectively. To verify test concentration and bioactivity of the 
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Cry1A.105 protein, samples of the test material were taken at test initiation. A bioassay using 

Helicoverpa zea was conducted to test for bioactivity and no significant difference was observed 

between the test substance and the Cry1A.105 reference standard. 

Conclusions: This study is acceptable. The NOEC for Cry1A.105 protein to honey bee larvae 

was determined to be at least 12 g Cry1A.105 protein/cell. 

MRID 469514-20 

An acute bioassay was conducted to determine the effects of Cry1A.105 protein on adult 

honeybees (Apis mellifera). The study was initiated a total of five times, with the first four 

attempts resulting in early termination (high control mortality). Adult bees were approximately 

five days old at the start of the bioassay. The test material was E. coli-produced Cry1A.105 

protein supplied by the study sponsor. The protein was used to prepare a 30% sucrose solution 

containing 550 μg Cry1A.105 protein/mL. A buffer control diet was prepared by combining the 

buffer solution with stock sucrose solution producing a 12.5 mM buffer in a 30% sucrose 

solution. An assay control diet was also prepared and consisted of only the 30% sucrose 

solution. A positive control diet was prepared and contained 100 μg/mL potassium arsenate in a 

solution of 30% sucrose. Honeybees were maintained in cages that were approximately 12.7 cm 

on each side. To induce clustering, a small cone of beeswax was attached to the cage cover and 

extended down into the cage. The diet was provided via an inverted 12 mL glass vial fitted with 

a plastic screw cap containing two ~1.0 mm holes. Each treatment group included 270 adult 

honeybees in six replicates of 45 adult honeybees per replicate. The number of dead bees in each 

cage was assessed on a daily basis. The study acceptance criteria stipulated that the assay be 

terminated at 30 days or when the adult control mortality reached 30%. The 30% criterion was 

met between Day 18 and 19 and the study was terminated on Day 20. On Day 18, the buffer 

control treatment produced significantly higher mortality (37.41%) than either the sucrose 

(20.00%) or the Cry1A.105 treatments (20.37%). Mortality in the Cry1A.105 treatment was not 

statistically different than the sucrose treatment on Day 18. On Day 19, no significant 

differences were detected among the three treatment groups (buffer, sucrose, and Cry1A.105 

protein) with mortalities of 52.22%, 51.48%, and 47.04%, respectively. The potassium arsenate 

treatment resulted in 100% mortality by Day 2 of the study. A diet incorporation assay using the 

Cry1A.105 test diet was conducted to confirm the bioactivity of the protein. The biological 

activity of the test substance was evaluated using Helicoverpa zea and compared with the 

biological activity of a Cry1A.105 reference standard. In addition, the control substance and the 

assay control substance were evaluated in the diet incorporation assay. The bioassay confirmed 

the test diet contained the expected level of Cry1A.105 activity. 

Conclusions: This study is acceptable. The NOEC for Cry1A.105 protein fed to adult honey 

bees is at least 550 μg/mL. 
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 (f) Earthworm (MRID 46954-18) 

The objective of this study was to evaluate the potential effects of acute exposure of Cry1A.105 

protein administered to earthworms (Eisenia fetida) during a 14-day exposure period. In the test, 

earthworms were exposed to a single concentration of Cry1A.105 protein that was incorporated 

into an artificial soil substrate. The Cry1A.105 protein used in this study was E. coli produced. 

The concentration of the test substance was 178 mg Cry1A.105 protein/kg soil dry weight. A 

total of four control treatments were included in the study, including: 1) buffer solution control, 

2) assay control group containing neither test substance or buffer solution, 3) positive control 

group exposed to 15 mg chloroacetamide/kg dry soil and 4) additional positive control group 

exposed to 30 mg chloroacetamide/kg dry soil. Each treatment was replicated four times and 

each replicate contained 10 earthworms. Test chambers consisted of one-liter glass beakers 

covered with plastic wrap what was perforated for air exchange. The artificial soil was prepared 

in bulk by blending 70% sand, 20% kaolin clay and 10% sphagnum peat. Each test container 

contained 750 grams of prepared soil. The worms were not provided food during the test period. 

At test initiation (Day 0), the worms were placed on the surface of the soil and observed for 30 

minutes to assess burrowing behavior. On Days 7 and 14, the contents of each test chamber 

were removed to determine the number of surviving worms. On Day 7, following observations, 

the test soil was returned to the test chambers and the worms were placed on the soil surface in 

order to observe burrowing behavior. On Day 14, following observations and body weight 

determinations, surviving earthworms were euthanized. Samples of soil were collected from 

each treatment (except positive controls) and saved to verify the presence or absence of 

biological activity. This was done by conducting a bioassay with Helicoverpa zea. The 

bioactivity of the Cry1A.105 treated soil was also compared against a reference standard of 

Cry1A.105 provided by the study sponsor. There were no mortalities in the assay control group, 

buffer control group, or Cry1A.105 protein group. In the 15 mg chloroacetamide/kg reference 

group there was 48% mortality and in the 30 mg chloroacetamide/kg reference group there was 

100% mortality at test termination. A slight loss in average individual body weight from test 

initiation to test termination was noted in all test groups and was expected since the worms were 

not fed during the 14-day test. Losses in body weight in the Cry1A.105 protein test substance 

group were not statistically significant when compared to the control substance group. Analysis 

of the test soil showed that Cry1A.105 was present in the soil and was biologically active against 

Helicoverpa zea. 

Conclusions: This study is acceptable. The 14-day LC50 for earthworms was determined to be 

greater than 178 mg Cry1A.105 protein/kg dry soil. The NOEC was determined to be greater 

than 178 mg Cry1A.105 protein/kg dry soil. 

vi. Soil Fate (MRID 469514-28)

Soil organisms may be exposed to Cry1A.105 and Cry2Ab2 protein through contact with corn 

plant roots (by direct feeding), corn plant root exudates, incorporation of above-ground plant 

tissues into soil following harvest, or by soil-deposited pollen. Some evidence suggests that soils 
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which are high in clays and humic acids are more likely to bind Cry protein. However, neutral 

pH soils (above pH 5.6), that are typical of corn production sites, tend to have high microbial 

activity and microbes contribute to Cry protein degradation. Despite evidence that soils high in 

clay and humic acids may bind Cry proteins, and thus interfere with the microbial degradation 

processes, the weight of evidence indicates that Cry proteins do not accumulate in soil to 

arthropod-toxic levels. Nonetheless, the Agency required the following soil fate evaluations to 

support the MON 89034 Bt corn registration. 

A study of Cry protein degradation in soil evaluated clay, silt loam, and loamy sand soils that 

were spiked with Cry2Ab2 (0.60 μg/g) or Cry1A.105 (0.062 μg/g) protein and incubated under 

controlled conditions for four months. The soils were dosed with an approximately 500-fold 

excess of the maximum calculated protein concentrations in the field. Samples of the treated soils 

were collected eleven times during the incubation period and analyzed for protein content using 

western blot analysis (Cry2Ab2 only) and a corn earworm bioassay. Results indicated that 

Cry2Ab2 protein concentration decreased by 50% in about 1 to 6 days, and by 90% in about 3 to 

14 days in the three soils. The amount of Cry1A.105 protein decreased by 50% in about 2 to 7 

days and by 90% in about 7 to 19 days in the same soils. 

This study utilized field soil spiked with purified insecticidal protein. This approach is useful 

because dose responses can be easily quantified. But, the degradation and accumulation of Cry 

proteins found within decaying plant tissue may behave differently than proteins in artificially 

spiked soil. Thus, the presence of low levels of Cry protein in the soil (at or below the level of 

detection) is anticipated until all plant tissue is ‘mineralized’. The data reviewed here do, 

however, show that Cry proteins will be quickly degraded upon release from decaying plant 

tissue. More specifically, a study that evaluated Cry1Ab protein accumulation in a field with

three years of continuous Cry1Ab field corn production showed that the protein had not 

accumulated in soil to a level that would elicit a toxic response from ECB larvae, a species that is 

highly susceptible to Cry1Ab protein (Milofsky, 2006). 

As a result of FIFRA Scientific Advisory Panel recommendations and public comments, the 

Agency has been receiving three year soil fate studies for the currently registered Cry protein 

producing crops grown in a variety of soils and environmental conditions. The results of these 

studies show that there is no detectable Cry protein accumulation in agricultural soils during 

commercial planting of currently registered Cry protein producing crops. Therefore no additional 

long term soil degradation studies are required for Cry2Ab2 or Cry1A.105 proteins. 

vii. Effects on Soil Microorganisms 

 

Numerous published studies indicate that exposure to Cry protein produced in Bt PIP crop plants 

does not adversely affect soil microorganisms. 12Although a minimal transient increase and shift 

12 Blackwood CB, and Buyer JS (2004). Soil microbial communities associated with Bt and nonBt-corn in three soils. Journal of Environmental Quality, 33: 832–836. 
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in microbial populations may result from the presence of transgenic plant tissue in soil, no 

adverse effects have been attributed to the Cry protein. In addition, there are several ongoing U.S. 

Department of Agriculture and EPA Office of Research and Development funded research 

projects evaluating the effects of Cry protein crops on soil microbial flora. If adverse effects are 

seen from this or any other research, the Agency will take appropriate action to mitigate potential 

risks. 

With regard to the impact of genetically engineered crops on soil, it is important to note that 

agricultural practices themselves cause large changes in soil and soil microbial composition. 

Furthermore, factors such variations in seasons and weather, plant growth stage, and plant 

varieties, independent of their being genetically engineered, are also responsible for significant 

shifts in soil microbial communities. Most studies with genetically engineered crops to date have 

shown minor or no effects on soil microbes beyond the variation caused by the factors listed 

above 

 4. Horizontal Transfer of Transgenes from Bt Crops 

EPA has evaluated the potential for horizontal gene transfer (HGT) from Bt crops to soil 

organisms and has considered possible risk implications if such a transfer were to occur. Genes 

that have been engineered into Bt crops are mostly found in, or have their origin in, soilinhabiting bacteria. Soil is also the habitat of anthrax, tetanus and botulinum toxin-producing 

bacteria. Transfer of these genes and/or toxins to other microorganisms or plants has not been 

detected. Further, several published studies conducted to assess the likelihood of HGT have been 

unable to detect gene transfer under typical environmental conditions. Horizontal gene transfer to 

soil organisms has only been detected with very promiscuous microbes under laboratory 

conditions designed to favor transfer. 

As a result of these findings, which suggest that HGT is at most an artificial event, and the fact 

that Bt toxins engineered into MON 89034 were derived from soil-inhabiting bacteria, the EPA 

Brusetti, L., Francia, P., Bertolini, C., Pagliuca, A., Borin, S., Sorlini, C., Abruzzese, A., Sacchi, G., Viti, C., Giovannetti, L., 

Giuntini, E., Bazzicalupo, M., and Daffonchio, D. (2004). Bacterial communities associated with the rhizosphere of transgenic Bt 

176 maize (Zea mays) and its non transgenic counterpart. Plant and Soil, 266: 11–21. 

Griffiths, B.S., Caul, S., Thompson, J., Birch, A.N.E., Scrimgeour, C., Andersen, M.N., Cortet, J., Messean, A., Sausse, C., 

Lacroix, B., and Krogh, P.H. (2005). A comparison of soil microbial community structure, protozoa and nematodes in field plots 

of conventional and genetically modified maize expressing the Bacillus thuringiensis Cry1Ab toxin. Plant and Soil, 275: 135–

146. 

Icoz, I, and G. Stotzky (2007). Cry3Bb1 protein from Bacillus thuringiensis in root exudates and biomass of transgenic corn does 

not persist in soil. Transgenic Research, September 13, 2007. 

Saxena, D. and Stotzky, G. (2001) Bacillus thuringiensis (Bt) toxin released from root exudates and biomass of Bt corn has no 

apparent effect on earthworms, nematodes, protozoa, bacteria, and fungi in soil. Soil Biol. Biochem., 33, 1225–1230 
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has concluded that there is a low probability of risk from HGT of transgenes found in Cry1A.105 

and Cry2Ab2 producing corn.

 5. Gene Flow and Weediness Potential 

Movement of transgenes from crop plants into weeds is a significant concern, due to uncertainty 

regarding the effect that a new pest resistance gene may have on plant populations in the wild. 

Under FIFRA, EPA has reviewed the potential for gene capture and expression of Bt endotoxins 

by wild or weedy relatives of corn, cotton, and potatoes in the U.S., its possessions and/or 

territories. To date, Bt plant-incorporated protectants have been registered for use in agronomic 

plant species that do not have a reasonable possibility of passing their traits to wild native plants. 

Due to concern over the possibility that species related to corn (Zea mays ssp. mays), such as 

Tripsacum species and the teosintes, could be recipients of gene flow from genetically modified 

Z. mays, EPA conducted a thorough review of the scientific literature on what is known about the 

gene flow potential of Z. mays (Bt Reassessment Document, 2001). Conclusions gathered from 

this review process are as follows: 

 The potential for pollen-directed gene flow from corn to Eastern Gama Grass is 

extremely remote (DeWald et al., 1999). This is evidenced by the difficulty with which 

Tripsacum dactyloides x Z. mays hybrids are produced in structured breeding programs. 

Additionally, the genus Zea does not represent any species considered as serious or 

pernicious weeds in the United States or its territories (Holm et al.,1979). Any 

introgression of genes into this species as a result of cross fertilization with geneticallymodified corn is not expected to result in a species that is weedy or difficult to control. In 

many instances where hybridization has been directed between these two species, the 

resultant genome is lacking in most or all of the corn chromosomal complement in 

subsequent generations (Chester DeWald, personal communication, 1999). 

 Many of the Zea species loosely referred to as “teosintes” will produce viable offspring 

when crossed with Zea mays ssp. mays. None of these plants are known to harbor weedy 

characteristics and none of the native teosinte species, subspecies or races are considered 

to be aggressive weeds in their native or introduced habitats (John Schoper, personal 

communication, 1999). In fact, many are on the brink of extinction where they are 

indigenous and will be lost without human intervention. Further, none of the landraces or 

cultivated lines of Z. mays are considered to have weedy potential and are generally 

considered to be incapable of survival in the wild as a result of breeding practices (i.e. 

selection) during domestication of the crop. 

The October 2000 Scientific Advisory Panel agreed that the potential for gene transfer between 

corn and any receptive plants within the U.S., its possessions and territories was of limited 

probability and nearly risk free. As a result of these findings, EPA has determined that there is 
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no significant risk of gene capture and expression of Cry1A.105 and Cry2Ab2 proteins by wild 

or weedy relatives of corn in the U.S., its possessions or territories. 

 6. Impacts on Endangered Species 

The primary route of exposure to Cry1A.105 and Cry2Ab2 proteins in corn is through ingestion 

of corn tissue. There are no reports of threatened or endangered species feeding on corn plants, 

therefore such species would not be exposed to corn tissue containing Cry protein. Since 

Cry1A.105 and Cry2Ab2 proteins have not been shown to have toxic effects on mammals, birds, 

plants, aquatic species, insects outside the order Lepidoptera and other invertebrate species at the 

Estimated Environmental Concentration (EEC), a "may affect" situation for endangered land and 

aquatic species is not anticipated. In addition, EPA does not expect that any threatened or 

endangered plant species will be affected by outcrossing to wild relatives or by competition with 

such entities. Hybrid corn does not exist in the wild, nor are there wild plants that can interbreed 

with corn in the United States. 

Because of the selectivity of Cry1A.105 and Cry2Ab2 proteins for lepidopteran species, 

endangered species concerns are mainly restricted to the order Lepidoptera. Examination of an 

overlay map showing the county level distribution of endangered/threatened lepidopteran species 

(currently listed by the U.S. Fish and Wildlife Service) relative to corn production counties in the 

United States clearly indicated that any potential concern regarding range overlap with corn 

production was mainly restricted to the Karner blue butterfly (Lycaeides melissa samuelis). 

Research demonstrates that the Cry1A.105 and Cry2Ab2 proteins are selectively toxic to 

lepidopteran larvae at field concentrations and that the Karner Blue butterfly is the only 

endangered lepidopteran species that may be exposed to MON 89034 (via pollen). A model 

developed to assess the risk of Bt corn to Monarch butterfly larvae was used to assess the risk of 

MON 89034 to Karner blue larvae. Based on the LC50 value for larvae of the most sensitive 

known lepidopteran species (ECB) and the maximum estimated level of Cry protein in pollencontaminated food (9.27 μg/g fresh weight), the margin of safety was calculated to be >10X 

maximum estimated exposure of Karner blue larvae to corn pollen. These results indicate that 

cultivation of MON 89034 is not likely to pose a risk to endangered species. 

After careful review of available data, EPA determined that exposure of the Karner blue butterfly 

to harmful levels of MON 89034 corn plant tissues is not expected. Likewise, a review of the 

preferred habitats of other lepidopteranan species listed as endangered by the U.S. Fish and 

Wildlife Service indicated that exposure to harmful levels of Cry1A.105 or Cry2Ab2 protein 

would not take place. The main reasons for the lack of exposure are geographical and habitat 

limitations. These species are located in non-corn production areas and/or their habitat does not 

encompass agricultural areas. 

Likewise, other insect species in the orders Diptera, Hemiptera, Lepidoptera, Odonata and 

Orthoptera that are listed as endangered/threatened species are found in dune, meadow/prairie or 

open forest habitats and are not closely associated with row crop production, often times due to 
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the specificity of the habitat of their host plants. The reviewed toxicological data shows the 

relative insensitivity of a range of insects in non-lepidopteran orders to the Cry1A.105 and 

Cry2Ab2 proteins, indicating that MON 89034 maize hybrids are not likely to have detrimental 

effects on non-lepidopteran insects included on the endangered/threatened species list. 

In light of the above considerations (based on no spatial and temporal overlap), the Agency has 

determined that registered uses of MON 89034 corn will have No Effects (NE), direct or 

indirect, on endangered and threatened species or their habitat as listed by the United States Fish 

and Wildlife Service (USFWS) and the National Marine Fisheries Services (NMFS), including 

mammals, birds or terrestrial and aquatic plants and invertebrate species. Therefore, no 

consultation with the USFWS is required under the Endangered Species Act. 

 7. MON 89034 Corn Environmental Risk Assessment Conclusions

The EPA uses a Maximum Hazard Dose Tiered system for biopesticide non-target wildlife 

hazard assessment. When no adverse effects at the maximum hazard screening dose are 

observed on representative non-target species, the Agency concludes that there are no 

unreasonable adverse effects on non-target populations from the use of the pesticide. 

a) Direct Effects 

At present, the Agency is aware of no identified significant adverse effects of Cry protein on the 

abundance of non-target organisms in any population in the aquatic or terrestrial field 

environment, whether they are animals, plants, pest parasites, pest predators, or pollinators. 

Further, EPA believes that cultivation of MON 89034 corn may have fewer adverse impacts on 

non-target organisms than use of chemical pesticides for corn production, because under normal 

circumstances, MON 89034 corn requires substantially fewer applications of chemical 

pesticides, compared to production of non-Bt corn. Fewer chemical insecticide applications 

generally result in increased populations of beneficial organisms that control secondary pests, 

such as aphids and leafhoppers. In addition, no adverse effect on Federally listed endangered 

and threatened species is expected from the proposed lepidopteran-resistant corn registration. 

Further, EPA has determined that there is no significant risk of gene capture and expression of 

Cry1A.105 or Cry2Ab2 proteins by wild or weedy relatives of corn in the U.S., its possessions, 

or territories (see Section 5. “Gene Flow and Weediness Potential” above), available data do not 

indicate that Cry proteins have any measurable adverse effect on microbial populations in the 

soil (see Section vii. “Effects on Soil Microorganisms” above), nor has horizontal transfer of 

genes from transgenic plants to soil bacteria been demonstrated (see Section 4. “Horizontal 

Transfer of Transgenes from Bt Crops” above). In conclusion, this risk assessment finds no 

hazard to the environment at the present time from cultivation of MON 89034 corn for a timelimited registration. 
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b) Indirect Effects: 

The purpose of using PIP plants is the same as for any other pest management tactic, i.e., to 

reduce pest populations below economic injury levels. As a result the abundance of pest insects 

should be significantly reduced and this will have corresponding implications for those 

organisms that exploit these pests as prey and hosts. Thus, the potential for these indirect 

ecological effects on biological control organisms should not be regarded as a unique ecological 

risk associated with the PIP crop. Some reductions, however, should be expected if the pest 

management strategy is effective. Since PIP crops are often grown in vicinity with conventional 

crops to prevent resistance build-up by the target pest(s), specialist antagonists can persist in 

these ‘refuges’, in other crops and in non-crop habitats and retain the potential for recolonization 

of the PIP crop area. Based on these considerations, regulatory testing of the specialist predators 

and parasitoids of target pests may eventually be considered unnecessary. 

c) Supplemental Data Needed to Confirm MON 89034 Non-Target Hazard Assessment 

The Agency has sufficient information to believe that there is no risk from the proposed uses of 

MON 89034 corn to non-target terrestrial wildlife, aquatic, and soil organisms. The Agency has 

been frequently asking the registrants to conduct post-registration long term invertebrate 

population/community and Cry protein accumulation in soils studies as a condition of 

registration. The issue of long range effects of cultivation of these Cry proteins on the 

invertebrate community structure in corn fields has since been adequately addressed by the 

analysis of field studies performed during the last 10 years (Marvier, et al. 2007; Sanvido, et al. 

2007). No unexpected adverse effects on invertebrate community structure were reported. The 

Agency is in agreement with these conclusions. Similarly, no unexpected accumulation of Cry 

proteins in agricultural soils was seen in published studies (Icoz and Stotzky 2007; Sanvido, et 

al. 2007) and in numerous studies submitted directly to the EPA for the currently registered Cry 

proteins. (Milofsky, 2006; See Section vi. “Soil Fate” above). 

In light of recently published laboratory studies showing reduced growth in shredding caddis 

flies exposed to anti-lepidopteran Cry1A protein corn litter (Rosi-Marshall, et al. 2007), 

additional aquatic invertebrate data are required. The submitted Daphnia magna study is 

unacceptable because it is an 850 Series OPPTS Guideline study. The 48 hour duration of this 

study is not sufficient to detect mortality due to Bt proteins. It takes more than 48 hours for the 

target pests to succumb to the Cry proteins, therefore 48 hours is also not expected to show 

mortality or reproductive effects on Daphnia. A 7 to 14 day Daphnia study as per the 885 Series 

OPPTS Guidelines needs to be performed. The study may be submitted as a condition of 

registration. Alternatively, a dietary study of the effects on an aquatic invertebrate, representing 

the functional group of a leaf shredder in headwater streams, can be performed and submitted in 

lieu of the Daphnia study. 
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Table 6. Supplemental non-target data requirements for MON 89034 corn. 

Testing Category Type of Data

Aquatic invertebrate A 7 to14 day Daphnia study as per the 885 Series OPPTS Guidelines has to be 

submitted as a condition of registration. 

Alternatively, a dietary study of the effects on an aquatic invertebrate, 

representing the functional group of a leaf shredder in headwater streams, can be 

performed and submitted in lieu of the Daphnia study. 

 

8. Potential Interaction Between Cry1A.105, Cry2Ab2 and Cry3Bb1 Proteins 

 (MRID 469513-05 & 469513-06) 

 

The purpose of these studies was to characterize the potential for interaction between the 

lepidopteran-active proteins Cry1A.105 and Cry2Ab2 and the coleopteran-active protein 

Cry3Bb1. The Cry1A.105 and Cry2Ab2 proteins were tested alone and in combination with 

either the Cry3Bb1 protein against European corn borer (ECB, Ostrinia nubilalis) and corn ear 

worm (CEW, Helicoverpa zea) in diet incorporation studies. Also, the Cry3Bb1 protein was 

tested alone and with the Cry1A.105 and/or the Cry2Ab2 proteins, against the Colorado potato 

beetle (CPB, Leptinotarsa decemlineata). The activity of Cry1A.105 and Cry2Ab2 proteins 

was not significantly altered by the presence of Cry3Bb1, and the activity of Cry3Bb1 was not 

significantly altered by the presence of Cry1A.105 and/or Cry2Ab2. Collectively these data 

provide evidence that the proteins do not interact in an antagonistic or synergistic manner. This 

study, along with the interaction study between Cry1A.105 and Cry2Ab2 reviewed for the 

MON 89034 Experimental Use Permit indicate that MON 89034 x MON 88017 maize will not 

result in any unexpected interaction in an antagonistic, or synergistic manner with regards to 

target insects. It is therefore extremely unlikely that the Cry1A.105, Cry2Ab2 and Cry3Bb1 

proteins contained in a single plant will impart any hazard to non-target organisms exposed to 

these hybrids in the environment. 

E. INSECT RESISTANCE MANAGEMENT (IRM) 

1. IRM Assessment for the Initial Registration of MON 89034 and MON 89034 x MON 

88017 

This section presents the original assessment for MON 89034 (based on BPPD’s review -- see 

BPPD, 2007). Subsequent to registration, Monsanto submitted an amendment request with 

additional IRM data to support a 5% lepidopteran refuge. This amendment and BPPD’s 

assessment are detailed in section 2 of the Insect Resistance Management Assessment. Please 

note that the assessment in section 1 contains some IRM program elements that have been 

superseded by the amendment discussed in section 2. 
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Monsanto developed through the use of genetic engineering, MON 89034, a corn product that 

produces the Bacillus thuringiensis (Bt)-derived insecticidal proteins Cry1A.105 and Cry2Ab2. 

The Cry1A.105 toxin is a “chimeric” protein containing domains I and II and the C-terminal 

from Cry1Ac and domain III from Cry1Fa (domain III). The Cry2Ab2 protein is exactly the 

same as that currently expressed in Monsanto’s Bollgard II cotton. MON 89034 is protected 

from damage caused by larval feeding of Ostrinia nubilalis (European corn borer; ECB), 

Diatraea grandiosella (southwestern corn borer; SWCB) and Diatraea saccharalis (sugarcane 

borer; SCB), Spodoptera frugiperda (fall armyworm; FAW), and Helicoverpa zea (corn 

earworm; CEW). Monsanto presented data to support its proposed IRM plan for MON 89034. 

Monsanto wished to demonstrate that: (1) resistance to Cry1A.105 and Cry2Ab2 proteins in 

MON 89034 is expected to be at least partially recessive; (2) the probability of cross-resistance 

between Cry1A.105 and Cry2Ab2 is low; and (3) the level of both Cry1A.105 and Cry2Ab2 

produced in MON 89034 confer high level of control of susceptible target pests (in vitro and in 

planta). Monsanto originally proposed a 5% structured refuge in the U.S. Corn Belt (currently, 

a 20% structured refuge) and a 20% structured refuge in cotton growing regions (currently, a 

50% structured refuge) to mitigate insect resistance to the Cry1A.105 and Cry2Ab2 proteins. 

Simulation modeling was provided to support this plan. BPPD’s technical analysis of 

Monsanto’s proposed IRM plan for MON 89034 is described below. 

a) Assessment of the Probability of Cross-Resistance to the Cry1A.105 and Cry2Ab2 

Proteins 

The Cry1A.105 toxin is a “chimeric” protein containing domains I and II and the C-terminal 

from Cry1Ac and domain III from Cry1Fa (domain III). The Cry2Ab2 protein is exactly the 

same as that currently expressed in Monsanto’s Bollgard II cotton. There are a number of Bt

corn products on the market that produce the insecticidal proteins, Cry1Ab and Cry1Fa 

(potential cross resistance with Cry1A.105/Cry2Ab2 and Cry1Ab is discussed in section c,

“Impact of Prior Use of Cry1Ab-Expressing Bt Corn Products on MON 89034”). There are 

also Bt cotton products that produce the Cry1Ac, Cry1F, and Cry2Ab2 insecticidal proteins. 

Mathematical models indicate that the IRM values of a Bt corn product with two insecticidal 

proteins, like MON 89034, would be the greatest if there is a low probability of crossresistance (See Roush 1998). Cross-resistance is most likely when proteins share key 

structural features, which allows one resistance mechanism to confer resistance to more than 

one protein (Tabashnik, 1994; Gould et al., 1995). 

There are three models that have been proposed to explain the mode of action of Cry1A toxin 

mode of action (see discussion in Piggott and Ellar, 2007). The most accepted Bravo model 

proposes that both the cadherin and aminopeptidase (APN) receptors are required for full 

Cry1A toxicity. This model suggests that receptor binding is sequential: 1) ingestion of the 

protein inclusions by a susceptible insect larva, 2) solubilization of the protein in the insect 

midgut, 3) cleavage of the protoxin by host proteases and release of the active toxin, 4) 

binding of the active toxin to specific receptors on the midgut epithelieum, 5) 
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oligomerization of toxin subunits to form pore structures that inject into the membrane, 6) 

passage of ions and water through the pores, resulting in swelling, lysis, and the eventual 

death of the host. Differences in any of these steps will reduce the probability of crossresistance between any two Cry proteins. The more controversial Zhang model suggests that 

receptor binding activates an Mg+-dependent signaling cascade that promotes cell death. The 

Jurat-Fuentes model suggests that cytotoxicity is due to the combined effects of osmotic lysis 

and cell signaling. The latter two models are, at present, more speculative. 

Resistance associated with modification of the binding site receptor has been the primary Bt

resistance mechanism reported to date (reviewed in Ferré & Van Rie 2002). Other Bt

resistance mechanisms have been reported that are based on alterations in the proteases that 

cleave the protoxin, processing it into a smaller active toxin (Candas et al. 2003) and most 

recently, the discovery that esterases can bind and detoxify Bt toxins (Gunning et al. 2005). 

Only the binding reduction mechanism has a demonstrated causal link between the 

biochemical modification and resistance (Ferré and Van Rie 2002). Ferré and Van Rie 

(2002) indicate that in all cases of binding site modification, resistance is due to a recessive 

or partially recessive mutation in a major autosomal gene, and cross-resistance extends only 

to Cry proteins sharing binding sites. Cry proteins that do not share high levels of sequence 

similarity tend to have different binding sites and different modes of action. Analyses of 

resistance to Bt Cry proteins indicate that cross-resistance occurs most often with proteins 

that are similar in structure (Tabashnik, 1994; Gould et al., 1995).

With this information in mind, Monsanto has assessed the probability of cross-resistance 

between Cry1A.105 and Cry2Ab2 on three levels: 1) structural similarity between the 

proteins, which is indicative of mode of action; 2) characterization of elements of the mode 

of action, such as the biophysical nature of binding of the Bt proteins to the target insect 

midgut; and 3) demonstration that the individual proteins are effective in controlling 

resistance to the other protein. Results of these efforts are discussed below. 

The first piece of the analysis relates to whether the Cry1A.105 protein has high sequence 

similarity with the Cry2Ab2 protein. Monsanto provided BPPD with a summary of current 

information about the structural and functional similarities of the Cry1A.105 protein to other 

Bt Cry1 proteins. The Cry1A.105 protein is a chimeric protein with overall amino acid 

sequence identity to the Cry1Ac, Cry1Ab and Cry1Fa proteins of 93.6, 90.0 and 76.7%, 

respectively. The Cry1A.105 protein expressed in MON 89034 corn plants results in 

increased activity against FAW, SCB, and CEW compared to Cry1Ab expressed in MON 

810 corn plants (see BPPD review of efficacy data, Matten, 2007; Monsanto study MRID# 

46951415). A structural model of the Cry1A.105 protein was developed using the X-ray 

crystal structure of the Cry1Aa protein. This model demonstrated high overall main chain 

structural similarity with Cry1Aa. Models of Cry1Ab and Cry1Ac were also prepared using 

the Cry1A.105 model. Comparison of the aligned folds of all three proteins showed that 

Cry1Ab and Cry1A.105 have essentially the same main chain structure (i.e., similar three 

domain structures) and that Cry1Ac differs slightly in its main chain structure from the other 
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two in domain III. Thus, comparison of the modeled crystal structures of the Cry1A.105, 

Cry1Ab, and Cry1Ac with the experimental Cry1Aa X-ray crystal structure demonstrated 

high three-dimensional structural similarity between the four proteins (i.e., Cry1A.105, 

Cry1Ab, Cry1Ac, and Cry1Fa). 

In the case of Cry1A.105 and Cry2Ab2 proteins, however, there is only a 14% amino acid 

sequence similarity. Based on the available data, Monsanto has sufficiently demonstrated 

that there is low sequence similarity between the Cry1A.105 protein and the Cry2Ab2 

protein. Lack of sequence similarity would suggest that cross-resistance between the 

Cry1A.105 and Cry2Ab2 proteins would be unlikely. On the other hand, high sequence 

similarity between the Cry1A.105 protein and Cry1Aa, Cry1Ab, Cry1Ac, and Cry1Fa 

proteins is one indicator that cross-resistance may be a concern for these proteins. This is 

important because Cry1A.105 is composed of domains I and II and the C-terminus of 

Cry1Ac and domain III of Cry1Fa. This subject will be discussed further in the review. 

Previous studies have shown that Cry1A proteins are activated by proteolytic cleavage of the 

C-terminal domain and the N-terminus of domain I in the insect gut. In contrast, Cry 2A 

proteins are activated by cleavage of the N-terminus of domain I and the C-terminal part of 

domain III. Different activation mechanisms would tend to decrease the likelihood of crossresistance between the Cry1A and Cry2A proteins. 

Assessment of binding characteristics is one way of determining the potential for crossresistance between the two proteins. As noted above, changes in the nature of protein 

binding to the insect midgut is the mode of action step that has most often been associated 

with insect resistance to Bt Cry proteins (for reviews, see Tabashnik, 1994; Baxter et al., 

2005). Biacore is used to quantify the interaction kinetics of Bt proteins with the insect brush 

border membranes (BBM). Competitive and non-competitive binding may not always be 

distinguished by Biacore and other analyses, such as ligand blotting, may be used. Ligand 

blotting is a qualitative tool used to identify protein bands that have the specific secondary 

modification to bind Bt proteins. Monsanto used both Biacore and ligand blotting to 

characterize Cry1A.105 and Cry2Ab2 binding to ECB brush border membranes (studies by 

Li and Guzov, 2006 were provided in Appendix 1 of Monsanto submission, MRID# 469514-

30, Head, 2006 and are discussed below). 

Binding constants for the interaction of Cry1A.105 and Cry2Ab2 with immobilized BBMV 

(ECB) differed by more than an order of magnitude with essentially no BBMV-specific 

binding being observable for Cry2Ab2. The Biacore system could not distinguish unique 

aspects of non-competitive binding for Cry1A.105 and Cry2Ab2 on BBMV. This result 

suggests that there are very different binding sites for Cry1A.105 and Cry2Ab2 in the ECB 

midgut. Additional Biacore analyses indicated that Cry1A.105 and Cry2Ab2 bound to 

different glycosyl moieties linked to bovine serum albumin (BSA). Cry1A.105 preferentially 
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bound to galactosamine (KD=1.5x10-8M and Rmax=2419 RU). Cry2Ab2 preferentially bound 

to N-acetyl glucosamine (KD=7.0x10-11M and Rmax=32 RU), but also bound galactosamine 

with a KD =2.0 x10-8M and Rmax=625 RU. Furthermore, Cry1A.105 binding to 

galactosamine filled a two-binding-site model as evidenced by the reduction in the Chi2 value 

from 2583 to 53, but the fit of Cry2Ab2 binding was similar for both models suggesting that 

the Cry2Ab2 and Cry1A.105 proteins not only bind to different sugars but also differ in their 

binding kinetics. 

 

The ligand blotting analysis demonstrated that the Cry1A.105 and Cry2Ab2 proteins bound 

to different components on ECB brush border membrane filaments (BBMF) separated by 

SDS-Page and immobilized on a nitrocellulose membrane. Trypsin-treated Cry1A.105 

protein was shown to bind to a ~150 kDa band while the trypsin-treated Cry2Ab2 protein 

was shown to a ~130 kDa band, but weakly to a ~150 kDa band. The trypsin-treated 

Cry2Ab2 protein had a greater rate of binding than the Cry1A.105 protein. Overall these 

results support the conclusion, as Monsanto has described, that the Cry2Ab2 and Cry1A.105 

proteins displayed different binding components and different kinetics in binding to ECB 

BBMF. These results are consistent with the differences in binding affinity for Cry1A.105 

and Cry2Ab2 proteins observed with Biacore. In addition, Monsanto noted that Cry2Aa did 

not bind to a specific, high affinity Cry1Ac receptor in work performed by English et al. 

(1994). 

In conclusion, Biacore and ligand blotting analyses demonstrate that Cry1A.105 and 

Cry2Ab2 proteins bind to some unique components on ECB brush border membranes. They 

also share many common binding sites. Screening a limited number of glycosylated BSAs, 

indicated that galactosamine is recognized by Cry1A.105 only, while Cry2Ab2 demonstrated 

a high affinity for both N-acetylglucosamine and galactosamine. These data support the 

conclusion that Bt protein binding to carbohydrate moieties is the principal basis of the 

specific interactions between the Cry1A.105 and Cry2Ab2 proteins and the ECB brush 

border membrane. Specific binding of Bt proteins to the target insect gut membrane is a key 

step in their mode of action. Differences in the Cry1A.105 and Cry2Ab2 protein interactions 

with the BBM suggest that these two proteins have differences in mode of action. BPPD 

agrees with Monsanto that these differences should minimize the development of crossresistance by the target insect pests to these two proteins. 

Monsanto also provided evidence to show that there is a lack of cross-reactivity between 

Cry1A.105 and Cry2Ab2 antibodies. The homologous primary-secondary antibody pairs 

recognized only their corresponding antigens (i.e., trypsin-treated Cry1A.105 or Cry2Ab2) 

with no cross-reactivity. Similarly, Monsanto previously demonstrated that anti-Cry2Ab 

antibodies do not cross-react with the Cry1Ac proteins, nor do the anti-Cry1Ac antibodies 

cross-react with the Cry2Ab2 protein (Head and Reding 2001, MRID# 455457-01). The lack 

of cross-reactivity shows that the epitope binding sites for antibody recognition are different 

and therefore the tertiary structure is different. Lack of similar tertiary structure supports the 
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conclusion that there will be a very low likelihood of high levels of cross-resistance in the 

target insect pests for the Cry1A.105 (and all Cry1A proteins) and Cry2Ab proteins. 

Monsanto provided indirect information (i.e., there are no colonies of lepidopteran corn pests 

resistant to either Cry1A.105 or Cry2Ab2 proteins) to indicate that insects resistant to one of 

the two insecticidal proteins, Cry1A.105 or Cry2Ab2, will be controlled by the other 

insecticidal protein. First, Monsanto cited to studies provided in support of the Bollgard II 

cotton registration (i.e, Cry2Ab2 and Cry1Ac Bt plant-incorporated protectants as expressed 

in cotton) that indicated that Cry1Ac-resistance did not confer Cry2Ab2 resistance to tobacco 

budworm, cotton bollworm, and pink bollworm (Head and Reding 2001; EPA 2007). In 

addition, Monsanto shared information that a Cry2Ab2-resistant colony (called SP15) of 

Helicoverpa armigera (Dr. Rod Mahon, CSIRO, Australia) showed little or no crossresistance to Cry1Ac and the microbial insecticide, DiPel®, that contains the Cry1Ab, 

Cry1Ac, and Cry2Aa proteins. Monsanto tested this Cry2Ab2-(SP15) resistant colony 

against purified Cry1A.105, Cry1Ac, and Cry2Ab2 protein relative to a susceptible 

laboratory colony of H. armigera. The SP15 colony was found to be highly resistant to the 

Cry2Ab2 protein, but showed little or no cross-resistance to the Cry1Ac and Cry1A.105 

proteins. Other published research indicates that there is evidence for broad cross-resistance 

(low levels of resistance) to Cry1A and Cry2A proteins in laboratory-selected strains of beet 

armyworm (Moar et al. 1995) and tobacco budworm (Gould, et al., 1992). Collectively, 

results of resistant colony studies indicate that there is some low potential for crossresistance, but that high levels of cross-resistance to Cry1A.105 and Cry2Ab2 is unlikely. In 

the field, this would translate to the efficacy of MON 89034 being maintained even though 

resistance might occur to one of the proteins. 

b) Dose 

The determination of dose, or the amount of toxin expressed by the transgenic crop relative 

to the susceptibility of the target pests, is a critical component of IRM. Models have shown 

that a high dose of toxin, coupled with a non-transgenic refuge to provide a supply of 

susceptible insects, is the most effective strategy for delaying resistance in Bt crops. The 

high dose/refuge strategy assumes that resistance to Bt is recessive and is conferred by a 

single locus with two alleles resulting in three genotypes: susceptible homozygotes (SS), 

heterozygotes (RS), and resistant homozygotes (RR). It also assumes that there will be a low 

initial resistance allele frequency and that there will be extensive random mating between 

resistant and susceptible adults. In practice, a high dose PIP should express sufficient 

quantities of toxin to kill all susceptible insects (SS) as well as heterozygous insects with one 

resistance allele (RS). Lower dose PIPs might allow for survival of insects with at least one 

susceptibility allele (SS or RS), although effective IRM may still be possible with a suitable 

refuge strategy. 

The 1998 Science Advisory Panel (SAP) defined high dose as a level of toxin 25 times 

greater than is needed to kill all susceptible insects. The SAP also outlined five techniques to 

determine high dose: 1) Serial dilution bioassay with artificial diet containing lyophilized 
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tissues of Bt plants using tissues from non-Bt plants as controls; 2) Bioassays using plant 

lines with expression levels approximately 25-fold lower than the commercial cultivar 

determined by quantitative ELISA or some more reliable technique; 3) Survey large numbers 

of commercial plants in the field to make sure that the cultivar is at the LD99.9 or higher to 

assure that 95% of heterozygotes would be killed (see Andow & Hutchison 1998); 4) 

Similar to #3 above, but would use controlled infestation with a laboratory strain of the pest 

that had an LD50 value similar to field strains; and 5) Determine if a later larval instar of the 

targeted pest could be found with an LD50 that was about 25-fold higher than that of the 

neonate larvae. If so, the later stage could be tested on the Bt crop plants to determine if 95% 

or more of the later stage larvae were killed. 

It must be noted that both the high dose definition and verification techniques were 

developed in 1998 when all of the registered Bt crops were single toxin products targeted 

against lepidopteran pests. In recent years, PIPs (in Bt cotton) have been approved that 

contain two genes targeted at the same insect pest. These “pyramided” products can be 

beneficial for IRM, since target pests must overcome two toxins to develop field resistance to 

the PIP. The benefits are greatest for two toxins with unrelated modes of action (i.e. binding 

to different Bt receptor sites in the midgut) that are expressed at high doses in the plant 

(Roush 1994). 

For pyramided products, the dose of each toxin should be evaluated separately. This can be 

easily accomplished if the pyramided product is created through conventional breeding -- in 

this case, the dose of the single toxin products has already been established and the combined 

dose in the pyramided PIP can be determined with comparative efficacy studies. But, for 

pyramids created by non-conventional breeding (e.g. recombinant DNA techniques), defining 

the dose can be more complicated since single toxin lines may not be available (or 

commercialized) for comparisons. The dual toxins can also be evaluated collectively to 

determine an “effective” high dose. In some examples, each toxin by itself may not supply a 

high dose, but in combination a sufficient control (>95% of heterozygotes) is provided to be 

considered high dose. 

MON 89034 was created with recombinant DNA technology (and not conventional breeding) 

to express the Cry1A.105 and Cry2Ab2 toxins. Both of the toxins are located on the same 

plasmid in the MON 89034 plant genome. Because of this, there are no originating single 

gene lines (i.e., expressing Cry1A.105 or Cry2Ab2 only) for dose comparisons, although 

single gene events were separately engineered. The Cry1A.105 toxin is a “chimeric” protein 

containing domains I and II and the C-terminal from Cry1Ac and domain III from Cry1Fa 

(domain III). By creating this chimera, Monsanto hoped to improve efficacy against several 

target pests including fall armyworm and corn earworm. The Cry2Ab2 protein is exactly the 

same as that currently expressed in Monsanto’s Bollgard II cotton. 

To evaluate dose, Monsanto conducted a number of laboratory and field studies with diet 

bioassays and MON 89034 plant material. Three sets of experiments were conducted: 1) 
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bioassays with purified toxin incorporated into artificial diet to determine pest susceptibility, 

2) leaf disk or kernel testing conducted in the laboratory, and 3) field tests with whole plants 

(artificial infestation of small corn plots) compiled over a several year period. Four target 

pests were evaluated including European corn borer (ECB), southwestern corn borer 

(SWCB), fall armyworm (FAW), and corn earworm (CEW). A description of the test 

procedures is included in Monsanto’s submission (Head 2006; MRID# 469514-30). Toxin 

expression data was also obtained from MON 89034 leaf tissue and other tested lines. 

Laboratory bioassays (Head 2006; MRID# 469514-30, section 2.2.1) were conducted using 

purified protein in diet to determine susceptibility (molting inhibitory concentration, MIC90) 

to the MON 89034 toxins. Molting inhibition is often used instead of straight mortality (i.e. 

an LC50 or LC90) because it can be assumed that insects that fail to develop as larvae will be 

functionally dead in the field. A MIC90 bioassay can also reduce the amount of purified toxin 

needed for the testing relative to an LC90 determination, though it is unclear whether 

Monsanto had insufficient purified protein to determine LC90 values. The MIC90 tests 

showed that all four target species were more susceptible to Cry1A.105 than Cry2Ab2 (as 

measured in ppm). ECB was more sensitive to both Cry1A.105 and Cry2Ab2 than the other 

tested lepidoptera by at least an order of magnitude. For Cry1A.105, BPPD agrees with 

Monsanto that the amount expressed in plant leaf tissue is high relative to the susceptibility 

of the target insects. Toxin levels in leaf tissue measured throughout the growing season (V2 

- Pre-VT) exceeded the MIC90 for all four species (both measured in ppm). On the other 

hand, the amount of Cry2A2b expressed in MON 89034 exceeded the MIC90 value only for 

ECB. For the other three pests, the level of Cry2Ab2 was at (for SWCB) or below (CEW 

and FAW) the MIC90 level. These data suggest that the Cry1A.105 component of MON 

89034 may be expressed at a sufficient level for all four pests to be considered “high dose” 

while the Cry2Ab2 expression is less certain. However, BPPD concurs with Monsanto’s 

contention that the results of laboratory bioassays are difficult to correlate with natural field 

systems and larval survival on plant tissue is more challenging than on artificial diet. 

Unlike the artificial diet bioassays, the tests with plant material (leaf disks and whole plant) 

directly assessed the performance of MON 89034 against the target pests. Since MON 

89034 expresses both Cry1A.105 and Cry2Ab2 simultaneously, the tests with MON 89034 

plant material evaluate the “effective dose” of both toxins together. However, Monsanto was 

also able to include single gene lines producing either Cry1A.105 or Cry2Ab2, though none 

of these were ultimately commercialized or used to create MON 89034. To relate the single 

gene isolines to MON 89034, Monsanto supplied some plant expression data for the isolines 

which could be compared to the known toxin expression of the stacked product. For the leaf 

disk/kernel tests, two Cry1A.105 isolines were used; one (LAJ138) with toxin expression 

equivalent to that of MON 89034 and another (LAJ129) with less than half the expression. 

Both of the Cry2Ab2 lines that were used (70774 and 67620) had less toxin expression than 

in MON 89034. Other single gene lines were used for the field tests, although no expression 

data were reported for those hybrids. 
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The results of the leaf disk tests generally supported the conclusions derived from the 

susceptibility diet bioassays (i.e. high efficacy against the target pests). Two-toxin MON 

89034 was highly effective against all four target pests with at least 90% mortality among 

exposed larvae and significant growth inhibition in the survivors. On the other hand, 

mortality was more variable for the single gene isolines that were also tested. For ECB, both 

MON 89034 and the single gene (Cry1A.105 and Cry2Ab2) isolines killed nearly all exposed 

larvae. Low survival (4%) was noted only on a Cry2Ab2 isoline (67620) and on MON 

89034, though the surviving larvae were stunted (< 41% the mass) relative to larvae on 

control leaf disks. The highest level of survival was noted for SWCB with some survival (up 

to 41% of the control group) observed on both the isolines and MON 89034, although the 

surviving larvae showed growth inhibition in all cases. For FAW and CEW, no survival was 

noted on MON 89034 or the isolines (though CEW survival on the control was only 26%, 

presumably due to CEW preference for feeding on corn ears instead of leaf tissue). 

A second trial using kernels instead of leaf disks was performed for CEW. This test revealed 

relatively high survival (up to 35%) on the lower expressing isolines (LAJ129 and 67620) 

and 9% survival on MON 89034 (growth inhibition was not recorded). 

 

Several sets of field tests (conducted in 2000 and 2002) showed high efficacy, though they 

provided less information on dose. The field tests were targeted primarily at ECB (one study 

was designed for SWCB) and assessed plant damage (as opposed to directly evaluating 

mortality). Single gene isolines were used, but no expression data were given (they were 

claimed to be lower than MON 89034) and MON 89034 was not included in the trials. The 

trials showed that Cry1A.105 and Cry2Ab2 isolines significantly reduced ECB and SWCB 

leaf and tunneling damage relative to the non-Bt control groups. Feeding damage was 

comparable to the commercial product MON 810, which is known to express a high dose for 

ECB and SWCB. While these studies demonstrated field efficacy of the Cry1A.105 and 

Cry2Ab2 isolines, they provide limited information for the assessment of MON 89034 dose. 

This is because 1) MON 89034 was not evaluated in any of the trials, 2) mortality was not 

assessed, and 3) CEW and FAW were not included in the trials. Monsanto recognized the 

limitations of the field work, but indicated that they should be considered in toto with the 

laboratory bioassays and leaf disk tests. 

Overall, the dose studies presented a mixed picture of the dose profile for MON 89034. Dose 

and efficacy data indicated that: (1) the Cry1A.105 and Cry2Ab2 proteins in MON 89034 

each provide essentially 100% control of ECB; (2) the CrylA.105 protein in MON 89034 

provides approximately 95% control of SWCB, while the Cry2Ab2 protein provides 80-90% 

control; (3) the Cry1A.105 and Cry2Ab2 proteins in MON 89034 each provide >95% control 

of FAW; and (4) the CrylA.105 and Cry2Ab2 proteins in MON 89034 each provide 90-95% 

control of CEW. Clearly, the hybrid offers a high level of control against the four major 

target pests including greater than 95% control of ECB and FAW and greater than 90% 

control of CEW and SWCB. The actual level of control may be even higher due to growth 

inhibition among survivors that would likely preclude developmental completion. As 
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demonstrated in the diet bioassays, the target pests appear to be somewhat more sensitive to 

Cry1A.105 than to Cry2Ab2. However, much of the dose information is circumstantial; the 

leaf disk assays were the only trial phase that directly evaluated MON 89034. The other data 

were obtained from susceptibility assays with purified protein (that were compared to MON 

89034 expression data) and tests with (non-commercialized) single gene isolines. 

BPPD agrees with Monsanto that MON 89034 provides strong control; these tests 

demonstrate that MON 89034 will likely kill >90% of susceptible insects. On the other hand, 

the data did not support a high dose under the definition put forth by the 1998 SAP (a level of 

toxin 25 times greater than needed to kill susceptible larvae; i.e. a dose greater than the LC99

of the pest). Some survival of MON 89034 plant tissue was noted for ECB, SWCB, and 

CEW. Monsanto assumed that the survivors would not reach adulthood due to growth 

inhibition (and therefore are functionally dead), but that assumption was not tested due to the 

short time frame of the experiment. 

Monsanto’s dose studies did not directly evaluate the effect of MON 89034 on potentially 

heterozygous larvae (i.e. with one copy of a resistance allele). Since heterozygotes may be 

more tolerant of Bt toxins than susceptible homozygous larvae, the 1998 SAP indicated that a 

high dose product should kill at least 95% of homozygous susceptibles. Roush’s modeling 

(1998) specifies that 70% of heterozygotes should be killed by the toxins expressed in the 

dual gene PIP. Monsanto assumes that MON 89034 meets the criteria for the Roush model 

(i.e. 95% susceptible and 70% heterozygote mortality), but no empirical evidence was 

presented regarding potential heterozygote mortality. Given that the major support for 

Monsanto’s proposal to reduce corn refuge from 20% to 5% is the Roush model, BPPD 

recommended that Monsanto further investigate whether MON 89034 consistently has high 

mortality of susceptible homozygotes (>95%) and further investigate heterozygote mortality 

for MON 89034. BPPD recognizes that direct evaluations of heterozygote effects can be 

difficult, particularly if resistant colonies for the target pests are unavailable and given that 

there is no field resistance to either protein. Monsanto has not provided enough information 

to determine the “killing power” of each individual protein -- it would be useful to assess 

whether Cry1A.105 and Cry2Ab2, individually, will kill greater than 95% of the susceptible 

homozygotes. The 1998 SAP suggested several ways to estimate mortality for less 

susceptible larvae (i.e. heterozygotes) (EPA 1998). These techniques included testing larger, 

later instar larvae that may be less susceptible to the toxins or with PIPs expressing lower 

levels of toxin than the commercial event (see the discussion of the SAP recommendations at 

the beginning of this section). 

c) Impact of Prior Use of Cry1Ab-Expressing Bt Corn Products on MON 89034 

Monsanto examined the impact of prior use of Cry1Ab-expressing Bt corn products on MON 

89034 Bt corn. Cry1Ab-expressing Bt corn products have been on the U.S. market since 

1997 and planted on millions of acres. This selection pressure could result in increased 

Cry1Ab-resistant allele frequencies in lepidopteran corn pests, particularly those that are 
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more dependent on corn as a primary host such as ECB and SWCB. Should there be 

Cry1Ab-resistant insects that are cross-resistant to either the Cry1A.105 protein and/or 

Cry2Ab2 protein then IRM value of MON 89034 would be significantly reduced. Given that 

there is very high amino acid similarity between the Cry1Ab and Cry1A.105 proteins, the 

potential for cross-resistance between Cry1Ab and Cry1A.105 is an important consideration. 

In an earlier section of this assessment, BPPD concluded that there is a low likelihood of 

cross-resistance between Cry1A.105 (Cry1A proteins) and Cry2Ab2 proteins. 

European corn borer (ECB) populations have been monitored for susceptibility to Cry1Ab 

since the 1995 growing season (diagnostic concentration information has been collected 

since 1999). Since 1998, monitoring has also been required for corn earworm (CEW), 

southwestern corn borer (SWCB), and fall armyworm (FAW, sweet corn only) susceptibility 

to Cry1Ab. All of the Cry1Ab monitoring data through the 2000 growing season were 

reviewed by the Agency during the 2001 Bt crops reassessment (EPA 2001). Data for the 

2001 through 2005 growing seasons were independently reviewed by BPPD (see Reynolds 

2004a, 2004b, 2006; Milofsky 2007). Estimates of the frequency of Cry1Ab resistance in 

ECB indicate that Cry1Ab-resistant alleles capable of conferring ECB survival on a Bt corn 

plant are very rare (Andow et al. 2000; Bourguet et al. 2003, Stodola et al. 2006). The 

highest estimate of Cry1Ab resistance allele frequency in U.S. ECB populations was 

<4 X 10-4 at the 95% confidence level. The Cry1Ab resistance allele frequency has not 

increased significantly in frequency even with the ten years of widespread use of Cry1Abexpressing corn products. There have been no instances of Cry1Ab resistance capable of 

conferring survival on Cry1Ab-expressing Bt corn plants in annual monitoring of ECB, 

SWCB, and CEW (see ABSTC 2006 and BPPD’s technical review in Milofsky 2007). 

BPPD agrees with Monsanto that the frequency of Cry1Ab-resistance is very low and that it 

has not increased significantly in over ten years of widespread use of Cry1Ab-expressing 

corn products. 

Current knowledge about Bt toxin receptors is summarized in a recent review by Piggott and 

Ellar (2007). By far the most studied receptors have been lepidopteran receptors associated 

with Cry1A toxins. These authors summarized Cry toxins for which a putative receptor has 

been identified (see Table 2 in Pillar and Ellar 2007). The Cry1A proteins (Cry1Aa, Cry1Ab, 

Cry1Ac, Cry1Ba, Cry1Ca, Cry1Fa) all have aminopeptidase N receptors (APNs) that can 

serve as Cry-binding proteins that mediate pore formation, but their relevance to toxin 

susceptibility has not been demonstrated. Cry1Aa, Cry1Ab, and Cry1Ac also have cadherinlike receptors that have been shown to mediate Cry1A toxicity. Other putative receptors, i.e., 

alkaline phosphatases, glycolipids, BTR-270, P252, may also play a role in Cry1A toxicity, 

but further study is needed. While there has been progress in what is known about Cry1A 

toxicity, little is known about other Cry families, such as the Cry2A family. How pore 

formation confers toxicity requires further study. 

Monsanto characterized binding of Cry1A.105 and Cry1Ab proteins to ECB brush border 

membrane vesicles using Biacore. These studies (Li and English 2006) indicated that 
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Cry1A.105 and Cry1Ab occupy different binding sites on the ECB midgut epithelial 

membrane and therefore have distinct membrane binding mechanisms. Cry1Ab binding data 

suggest that the binding patterns are much more complex for Cry1Ab than for Cry1A.105 

despite these two proteins having 90% amino acid sequence homology. BPPD agrees with 

Monsanto that differences in binding mechanisms lessen the likelihood of Cry1A.105 and 

Cry1Ab cross-resistance. 

Monsanto also summarized the laboratory studies examining ECB colonies selected for 

resistance to the Cry1Ab protein. While these colonies are imperfect tools for predicting 

what will happen in the field, they are the best tools available for looking at potential 

resistance mechanisms. In particular, Monsanto discussed a series of studies conducted on 

three ECB colonies selected for resistance to Cry1Ab by Blair Siegfried at the University of 

Nebraska (Siegfried and Spencer 2001). Two of the colonies were created by laboratory 

selection, the Europe colony was established from larvae collected in Lombardia region of 

northern Italy and a second colony was created from larval collected in Nebraska. A third 

colony was created from survivors of diagnostic bioassays from both the Europe and 

Nebraska populations. All three colonies, along with two susceptible colonies, were assayed 

for their response to purified Cry1Ab, Cry1Ac, Cry2Ab2, and a version of the Cry1A.105 

protein. Results of the bioassays indicated that all three Cry1Ab-resistant colonies were 

resistant to Cry1Ab and Cry1Ac, but remained susceptible to Cry1A.105 and Cry2Ab2 with 

no evidence of cross-resistance. 

As noted earlier, Cry1A.105 is a chimeric protein consisting of domains I and II and the Cterminus of Cry1Ac and domain III of Cry1Fa. Several pieces of evidence suggest that there 

is at least some likelihood of cross-resistance of Cry1Fa and Cry1Ac/Cry1Ab. Denolf et al. 

(1993) conducted Cry1Ab, Cry1Ac, and Cry1B proteins binding experiments with isolated 

brush border membrane vesicles (BBMV) and gut tissue sections from ECB. These studies 

indicated that Cry1Ab and Cry1Ac proteins recognized the same membrane receptor with 

different binding affinities while the Cry1B protein recognized a separate receptor. More 

recent binding studies with BBMV from ECB conducted by Hua et al. (2001) indicated that 

there was limited shared binding between Cry1Fa and Cry1Ab/Cry1Ac proteins. Pereira et 

al. (2008) showed that there was little cross-resistance to Cry1Ac (6.9-fold) in a Cry1Faresistant line of ECB (>3,000-fold). Jurat-Fuentes and Adang (2001) demonstrated that 

Cry1Fa (and Cry1Ja) share the Receptor A binding site with the Cry1A toxins in Heliothis 

virescens (tobacco budworm), but they also have unique binding sites. These researchers 

proposed a model that suggests that Cry1Fa, Cry1Ab, and Cry1Ac all bind to the Cry1Aa 

binding site (called Receptor A, although with different binding affinities) as well as to 

unique binding sites. An altered Cry1Aa binding site may cause resistance to Cry1Aa, 

Cry1Ab, Cry1Ac, and Cry1Fa proteins, but the unique binding sites also play a role in 

toxicity. Competition binding experiments performed by Hernández and Ferré (2005) 

showed the occurrence of a common receptor for Cry1Ac, Cry1Fa, and Cry1Ja in 

Helicoverpa armigera, H. virescens, and Spodoptera exigua. So far, all available 

information on binding site competition suggests that Cry1Aa, Cry1Ab, Cry1Ac, Cry1Fa, 
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and Cry1Ja share a common binding site in most, if not all, Lepidoptera. These authors 

suggest that Cry1Aa, Cry1Ab, Cry1Ac, Cry1Fa, and Cry1Ja protein binding to a common 

site explains, perhaps, the biochemical basis of multiple resistance and cross-resistances 

among these five proteins in some insect species. Jurat-Fuentes and Adang (2006) recently 

demonstrated that a cadherin-like protein, HevCaLP, is the functional receptor for Cry1Ac 

binding in a highly-resistant (>300,000-fold) tobacco budworm colony (YHD2) although it is 

not a receptor for Cry1Fa (130-fold resistant). These results suggest that the Cry1Fa and 

Cry1Ac shared binding site is not a cadherin-like protein and that cross-resistance would be 

due to modification of some other receptor. Collectively, the availability information 

indicates that there is some likelihood of cross-resistance to both the Cry1Fa and 

Cry1Ab/Cry1Ac proteins through modification of a single shared receptor site. Hernández 

and Ferré (2005) suggest that neither transgenic plants expressing stacked combinations of 

Cry1Ac (and by extension Cry1Ab), Cry1Fa, and Cry1Ja nor rotations of Bt crops containing 

single genes of these three (four) proteins would be a good resistance management strategy. 

In the case of corn, primary pests susceptible to Cry1Ab and Cry1Fa, such as ECB (and 

SWCB and CEW), would necessitate the importance of establishing the binding site model 

for this species in order to develop an appropriate resistance management strategy.

 

Monsanto has shown, using the weight-of-evidence approach, that there is a low likelihood 

of cross-resistance between Cry1A.105 and Cry2Ab2 (see section a., “Assessment of the 

Probability of Cross-Resistance to the Cry1A.105 and Cry2Ab2 Proteins” of this IRM 

assessment). It is assumed that the primary mechanism of resistance will be that of binding 

site modification, which is a reasonable assumption based on studies with other Bt-resistant 

insect populations (laboratory and field) (see Ferré and Van Rie 2002). Similarly, Monsanto 

has adequately demonstrated that there is a low likelihood of cross-resistance of Cry1A.105 

and Cry1Ab. On the other hand, Monsanto has not addressed the likelihood of crossresistance of Cry1A.105 and Cry1Fa and Cry1Ac. The Cry1A.105 protein is a chimeric 

protein consisting of Domains I and II and the C-terminus of Cry1Ac and Domain III of 

Cry1Fa. 

It was recommended that Monsanto provide BPPD with additional information on crossresistance of Cry1A.105 and Cry1Fa and Cry1Ac (including binding site models and use of 

resistant colonies) for the target pests and determine how such cross-resistance may impact 

the durability of MON 89034. 

d) Proposed IRM Plan for MON 89034 

Monsanto stated that the introduction of MON 89034 would significantly decrease the risk of 

lepidopteran pests evolving resistance to Bt corn. Monsanto’s Insect Resistance Management 

(IRM) plan for MON 89034 focused on three key assumptions: (1) resistance to Cry1A.105 

and Cry2Ab2 is expected to be at least partially recessive; (2) the probability of crossresistance between Cry1A.105 and Cry2Ab2 is low; and (3) the level of both Cry1A.105 and 

Cry2Ab2 produced in MON 89034 confer high level of control of susceptible target pests 
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(high dose defined as at least 90% and preferably >95% control). Should these assumptions 

be met, MON 89034 would have significantly more durability than all existing single-gene 

products for lepidopteran control in the U.S., including MON 810 (Cry1Ab), BT11 

(Cry1Ab), Herculex I (Cry1Fa), and their respective stacked products. The primary focus of 

the MON 89034 IRM plan was on management of ECB resistance and to a lesser extent, 

SWCB and CEW in regions where these pests are economically important. FAW and 

sugarcane (SCB) were not a focus of Monsanto’s IRM for MON 89034. 

Monsanto’s proposed IRM plan for MON 89034 originally consisted of the following 

elements. 

1. A 5% structured non-lepidopteran Bt corn refuge for the Corn Belt based on two 

independent (minimal cross-resistance), highly effective modes of action of Cry1A.105 

and Cry2Ab2; 

2. A 20% structured non-lepidopteran Bt corn refuge for cotton-growing areas; 

3. Annual resistance monitoring, grower education, and compliance monitoring programs; 

and 

4. A remedial action plan that describes a series of action to investigate suspected 

resistance, confirms actual resistance, and mitigates the resistant population(s). 

Each of these elements will be discussed below. 

i. 5% Structured Refuge for Field Corn Uses of MON 89034 in the Corn Belt

The critical question is whether Monsanto has provided sufficient data/information to 

indicate that the durability of a 5% structured refuge (as Monsanto has proposed) is equal to 

or greater than durability of a 20% structured refuge (the current structured requirement for 

lepidopteran-protected Bt corn products) for management of resistance to MON 89034. 

Monsanto’s MON 89034 IRM plan for field corn uses focused on ECB, though the issue of 

whether MON 89034 has consistently high mortality of susceptible homozygotes for all of 

the primary target species also has to be considered. 

In the case of MON 89034, two Bt genes, cry1A.105 and cry2Ab2, were engineered into Bt

corn plants to provide even better control (than first-generation, single Bt protein products) of 

ECB, CEW, SWCB, and FAW. Two proteins are expressed in MON 89034 corn plants: 

Cry1A.105, a chimeric protein consisting of domains of Cry1Ac and Cry1Fa; and Cry2Ab2, 

the same protein that is expressed in Bollgard II cotton (a Monsanto product). Monsanto has 

provided sufficient efficacy data to demonstrate that MON 89034 provides good control of 

ECB, CEW, SWCB, FAW, and SCB (see Matten, 2007 for BPPD’s review of Monsanto’s 

submission, Headrick et al. 2006, MRID# 469514-15). The level of control of MON 89034 

for these pests was equal to or greater than YieldGard (MON 810, Monsanto’s single Bt

(Cry1Ab) trait corn product). 
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Monsanto’s first assumption was that Cry2Ab2 and Cry1A.105 have different modes of 

action and therefore the potential for cross-resistance is low. As discussed earlier in this 

review, Cry2Ab2 and Cry1A.105 have low sequence homology (14%), different activation 

mechanisms and binding characteristics, unique antibody binding sites, and resistant insects 

to one protein will be controlled by the other protein. Therefore, it can be concluded that 

Cry2Ab2 and Cry1A.105 have different modes of action and therefore it is expected that 

there will be a low likelihood of cross-resistance (see earlier discussion in Section a. 

“Assessment of the Probability of Cross-Resistance to the Cry1A.105 and Cry2Ab2 

Proteins”). Lack of cross-resistance would increase the durability of MON 89034. These 

two proteins, Cry2Ab2 and Cry1A.105, therefore, seem to be good candidate proteins for 

pyramiding. BPPD agrees with Monsanto that the probability of cross-resistance between 

Cry1A.105 and Cry2Ab2 is low and these two proteins have different modes of action. 

On the other hand, Roush (1998) cautions that proteins that have already shown significant 

levels of cross-resistance in resistant insect strains (e.g., H. virescens, H. armigera, S. exigua, 

O. nubilalis, Plutella xylostella), such as between Cry1A, Cry1Fa and Cry1J proteins, should 

not be used in pyramiding. This same warning was also given by Hernández and Ferré 

(2005). Cry1A.105 is a chimera that consists of binding domains of Cry1Ac and Cry1Fa. 

There are commercial Bt crops that express Cry1Ac, Cry1Ab, Cry1Fa proteins and these 

products have been in the marketplace for nearly a decade. Should there be insect 

populations resistant to Cry1Ac, Cry1Ab, and/or Cry1Fa that are cross-resistant to 

Cry1A.105 then the durability of MON 89034 would be significantly reduced and a 5% 

structured refuge would be insufficient to maintain high levels of durability. 

Given that there is very high amino acid similarity between Cry1Ab, Cry1Ac, and 

Cry1A.105 proteins then the potential for cross-resistance between Cry1Ab, Cry1Ac and 

Cry1A.105 is an important consideration. Cry1A.105 and Cry1Fa have about 76% amino 

acid similarity. However, what is really important is the similarity of the binding domain III 

of Cry1Fa and Cry1A.105 which is presumed to be very high. Cross-resistance is a real 

possibility for these two proteins. There are several lines of evidence that indicate that 

Cry1Fa and Cry1Ab/Cry1Ac share a common binding receptor although each of these 

proteins has unique binding receptors as well (Denolf 1993, Hua 2001, Jurat-Fuentes and 

Adang 2001; Hernández and Ferré, 2005). Evidence for a shared binding receptor would 

increase the likelihood of cross resistance should resistance evolve through modification of 

the shared binding receptor. 

 

Evidence provided by Monsanto indicates that there is little cross-resistance of Cry1A.105 

and Cry1Ab. One cannot, however, infer much about the likelihood of cross-resistance of 

Cry1A.105, Cry1Fa, and Cry1Ac based on the binding patterns of Cry1A.105 and Cry1Ab 

because binding patterns are unique to each species (e.g., ECB, SWCB, and CEW) and each 

protein. Monsanto did not address the likelihood of cross-resistance of Cry1A.105 and 

Cry1Fa, a protein already in existing Bt corn and Bt cotton products, and what impact crossresistance would have on the durability of MON 89034. BPPD recommends that Monsanto 
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provide additional information on cross-resistance of Cry1A.105 and Cry1Fa and Cry1Ac 

(including binding site models and use of resistant colonies) for the target pests and 

determine how such cross-resistance may impact the durability of MON 89034. 

Monsanto’s second assumption was that resistance will be recessive. Ten years of resistance 

monitoring data indicate that the frequency of Cry1Ab alleles in ECB is very low (<4 X 10-4) 

and that this frequency has not changed significantly during this time. The 20% structured 

refuge requirement for single-gene Bt corn products has been in place for over a decade and, 

as noted earlier in this review, there is no evidence of field resistance to Cry1Ab and Cry1Fa 

in ECB, SWCB, and CEW during that period in the continental U.S. There is also no 

evidence of Cry2Ab2 resistance (CEW) after five years of widespread use of Bollgard II 

cotton. The absence of any cases of field resistance to Bt crops after a decade of use 

indicates that any relatively common Bt-resistant alleles must be recessive (Tabashnik et al., 

2003). This evidence provides a strong indicator that resistance to the Bt proteins expressed 

in MON 89034 would also be recessive. BPPD agrees with this line of reasoning. Pyramids 

are considerably more effective when resistance frequencies are low provided that the 

susceptible homozygotes are all killed by each of the toxins used separately (Roush 1998; 

Figure 4.). 

Monsanto’s third assumption for its proposed 5% structured refuge depended on whether the 

amount of Cry1A.105 and Cry2Ab2 produced in MON 89034 confers a high level of control 

of susceptible target pests (defined as at least 90% and preferably >95% mortality). It is this 

third assumption that is the most difficult to prove. 

Resistance simulation models predict that the greatest benefits of combining toxins in single 

plants by “pyramiding” or “stacking” are achieved when no cross-resistance occurs, when 

there are no fitness costs, when resistance to each toxin is rare and recessive, and when a 

refuge of plants without toxins are present. Modeling simulations of two-gene products 

predict that the resistance risk associated with a two-gene product will be significantly less 

than for a single-gene product (for example, Caprio 1998; Roush 1998). Pyramiding two or 

more proteins increases the chance that at least one of the proteins will be especially 

favorable to resistance management. Modeling simulations predict that pyramids (without 

cross-resistance) can reduce the need for larger refuges (Roush 1998). 

Pyramiding relies on the idea that each protein is used individually in a way that would kill 

all insects susceptible to that protein, and in so doing, kills insects that are resistant to the 

companion protein (Roush, 1998). This has been described as “redundant killing” in the 

sense that most of the population is susceptible to both proteins and thus is killed twice. The 

extent to which the individuals that are resistant to one protein are killed by the other is 

central to the effectiveness of the pyramiding strategy. 

Monsanto relied on the Roush (1998) model to support the need for a 5% structured refuge 

rather than a 20% structured refuge in the Corn Belt. Roush’s model (figure 2 in the 
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publication) indicated that a 5% structured refuge is equal to or greater than a 20% structured 

refuge for a highly effective, high dose single-gene product when a two-gene product (MON 

89034 in this case) achieves at least 95% control of susceptible homozygotes and 70% 

control of heterozygotes assuming there is no cross-resistance. Monsanto’s dose studies, as 

discussed earlier, presented a mixed picture for MON 89034 (see Section b., “Dose”). Dose 

and efficacy data indicated that MON 89034 has a high level of control against the four 

major target pests (as described in Head 2006): “(1) the Cry1A.105 and Cry2Ab2 proteins in 

MON 89034 each provide essentially 100% control of ECB; (2) the CrylA.105 protein in 

MON 89034 provides approximately 95% control of SWCB, while the Cry2Ab2 protein 

provides 80-90% control; (3) the Cry1A.105 and Cry2Ab2 proteins in MON 89034 each 

provide >95% control of FAW; and (4) the CrylA.105 and Cry2Ab2 proteins in MON 89034 

each provide 90-95% control of CEW.” The actual level of control may be even higher due 

to growth inhibition among survivors that would likely preclude developmental completion. 

The target pests appear to be somewhat more sensitive to Cry1A.105 than to Cry2Ab2. 

Monsanto’s dose testing indicated that MON 89034 has a high level of control (greater than 

90%) of susceptible homozygotes (ECB, SWCB, CEW, FAW), one of two thresholds needed 

to support the durability of a 5% structured refuge for a two-gene pyramided Bt corn product 

(as equal to or better than that of a single-gene Bt corn product expressing a high dose of 

control against the target pests). However, it was not easily discernable as to whether each 

individual toxin kills greater than 95% of susceptible individuals. This is important for 

prediction of the durability of MON 89034: Roush’s simulations (1998; Figure 3) showed 

that the greatest gains of pyramiding two proteins are when the mortality of susceptible 

insects is considerably greater than 95%, especially if resistance allele frequencies are quite 

low. 

On the other hand, Monsanto’s dose studies did not directly evaluate whether MON 89034 

kills at least 70% of the heterozygotes, the other threshold needed to support a 5% structured 

refuge for a two-gene pyramided Bt corn product. Monsanto did not provide enough 

information to determine the mortality of susceptible (SS) homozygotes and heterozygotes 

(RS) on MON 89034 plants. It is important to know whether Cry1A.105 and Cry2Ab2 are: 

1) both high dose proteins; 2) one high dose and one moderate dose protein (and which one is 

high and which one is moderate); or 3) two moderate dose proteins to control ECB (and 

SWCB) in the Corn Belt. In other words, one has to establish whether each protein can kill 

potentially resistant individuals to the other protein. To evaluate MON 89034 in the context 

of Roush’s model, it must be determined whether Cry1A.105 and Cry2Ab2 are produced at 

high levels to kill at least 95% of susceptible homozygotes and 70% of the heterozygotes. 

Because of this, BPPD recommended that Monsanto further investigate heterozygote 

mortality for MON 89034. BPPD recognizes that direct evaluations of heterozygote effects 

can be difficult, particularly if resistant colonies for the target pests are unavailable. 

However, the 1998 SAP suggested several ways to estimate mortality for less susceptible 

larvae (i.e. heterozygotes). These techniques included testing larger, later instar larvae that 

may be less susceptible to the either the Cry1A.105 or Cry2Ab2 proteins or with PIPs 
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expressing less protein (less Cry1A.105 or Cry2Ab2) than MON 89034. As Roush (1998) 

cautioned, “...small refuges remain risky...” when mortalities of heterozygotes are lower 

than expected. For MON 89034, it has only been assumed (but not verified) that the 

heterozygote mortality will be at least 70% for each protein. 

Cross-resistance between Cry1A.105 and Cry1Ac and Cry1Fa is not known, but published 

studies indicate that there is at least some potential for cross-resistance between Cry1A and 

Cry1Fa proteins in a number of insect species (see earlier discussion). The impact of this 

potential cross-resistance on the durability of MON 89034 is not known. 

Monsanto’s use of the Roush (1998) model as a guide to predict the durability of MON 

89034 was very useful, but it is only a first step. Roush encouraged researchers to further 

investigate the points raised in his 1998 paper with additional modeling and experiments (see 

Roush 1998). However, this was not done by Monsanto. Additional modeling using a 

species-specific (e.g., ECB and SWCB for the Corn Belt), spatially-explicit, preferably 

stochastic, landscape model of available Bt crops expressing many different Cry proteins 

(needs to be a multiple gene model, a more complex model) needs to be performed to more 

precisely predict the evolution of ECB resistance (or SWCB) to MON 89034. This new 

model would need to consider the impact of other Bt proteins in which there may be some 

cross-resistance. This is analogous to the species-specific simulation modeling that EPA 

required Monsanto do to support the use of natural refuge (instead of a structured refuge) for 

management of H. virescens and H. zea to the Cry1Ac and Cry2Ab2 proteins expressed in 

Bollgard II cotton. In conclusion, Monsanto’s initial data and modeling do not support a 5% 

structured refuge for MON 89034 for field corn uses in the Corn Belt. 

Given the uncertainties in the dose determination for ECB and SWCB (SS and RS mortality) 

(note: CEW and FAW are lesser pests in the Corn Belt), cross-resistance likelihood of 

Cry1A.105, Cry1Ac, and Cry1Fa, and limitations of the simulation modeling, BPPD 

recommended that the current 20% structured refuge requirement for field corn uses of MON 

89034 in the Corn Belt be maintained until such time as Monsanto could address these 

uncertainties associated with the durability of a 5% structured refuge. There are many Bt

corn and Bt cotton products in the landscape. Cross-resistance conferred by any of these 

proteins may negatively affect the durability of MON 89034. Studies indicate that there is at 

least some potential for cross-resistance between Cry1A.105, Cry1Fa, and Cry1Ac proteins 

in a number of insect species (see earlier discussion). Monsanto needs to examine the 

potential of Cry1A.105, Cry1Ac, and Cry1Fa cross-resistance and what impact it has on the 

durability of MON 89034. 

ii. 20% Structured Refuge for Field Corn Uses of MON 89034 in Cotton-Growing Areas

Monsanto has proposed that a 20% structured refuge rather than the current 50% structure 

refuge requirement for single-gene lepidopteran-control products be used to manage insect 

resistance to MON 89034 in cotton-growing areas. The major pest of concern for Bt corn in 

cotton-growing areas is CEW (also known as cotton bollworm when it feeds on cotton), 
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although ECB, FAW, SCB (sugar cane borer) are also sporadic corn pests in cotton-growing 

areas. As described earlier in this assessment (Section b “Dose”), Cry1A.105 and CryAb2 

proteins have at least 90% control of CEW. Previous studies submitted by Monsanto (Head 

and Reding 2001; reviewed in EPA 2007) demonstrated the low likelihood of crossresistance between the Cry2Ab2 and Cry1Ac proteins. Both Cry1A.105 and Cry2Ab2 have 

a low likelihood of cross-resistance with Cry1Ab (see earlier discussion in Section c, 

“Impact of Prior Use of Cry1Ab-Expressing Bt Corn Products on MON 89034”). 

Monsanto used its deterministic, non-spatial model (Gustafson and Head 2005) to examine 

whether planting a 20% structured non-Bt corn refuge with MON 89034 was sufficient to 

manage the risk of resistance evolution to Bt corn and Bt cotton products. In this model, it 

was assumed that all cotton planted consisted of Bollgard II cotton, with no non-Bt cotton in 

the system, and that 80% of the corn planted in the region consisted of MON 89034 and 20% 

non-Bt corn. The modeling was focused on estimation of the likelihood of CEW resistance 

in the Mississippi region because of the relatively higher risk of CEW resistance evolution in 

this review. Monsanto estimated the effective (all non-Bt hosts of CEW, including current 

levels of non-Bt cotton and 20% non-Bt corn refuge associated with MON 89034) and natural 

refuge (only non-cotton hosts of CEW, including 20% structured non-Bt corn refuge 

associated with MON 89034 and other unmanaged hosts) available for CEW in this region as 

described in Gustafson and Head (2005). These estimates were used as parameter values in 

the model. One model scenario assumed that MON 89034 is fully cross-resistant with 

Bollgard II cotton (i.e., Cry1A.105 and Cry1Ac are fully cross-resistant). Resistance was 

assumed to be complete with no associated fitness costs. Using these assumptions, the 

simulation modeling predicted that a 20% non-Bt corn refuge for MON 89034 in the southern 

cotton-growing areas would be sufficient to manage the risk of resistance evolution to Bt

corn and Bt cotton products. Resistance to Cry2Ab2 protein evolved first and took >24 

modeling years to evolve (modeling time was 25 years). It is not clear from Monsanto’s 

discussion whether Cry1A.105 and Cry1Fa cross-resistance was included in the modeling. 

The current landscape has both Cry1Fa- and Cry1Ab-corn and Cry1Fa- and Cry1Ac- and 

Cry2Ab2 + Cry1Ac-cotton products. Should there be substantial cross-resistance, then the 

value of MON 89034 would be dramatically reduced. 

A 20% non-Bt corn refuge for MON 89034 in the southern cotton-growing areas would be 

sufficient to manage the risk of resistance evolution to Bt corn and Bt cotton products 

assuming there is no cross-resistance. Monsanto did not, however, sufficiently address the 

cross-resistance of Cry1A.105, Cry1Fa, and Cry1Ac in the cotton-growing landscape and 

how such cross-resistance may impact the durability of MON 89034. Should crossresistance be of concern then the durability of MON 89034 in the southern cotton-growing 

areas might be compromised. Monsanto needs to address this potential in subsequent 

simulation modeling. 

 
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e) Sweet Corn Uses 

As stated in Monsanto’s submission (Head 2006): “In the U.S., sweet corn is grown on 

approximately 500,000 acres, with California, Florida, Georgia, New York, Ohio, and 

Pennsylvania accounting for 62% of the acres. The insecticide use per acre on sweet corn is 

approximately 35-fold that of field corn (2.7 lb/A versus 0.76 lb/A) (USDA, 2006) and 

typically, 12 - 40 applications of insecticides may be applied to a single crop of sweet corn in 

the southern U.S (Adams 1996). Therefore, planting of MON 89034 has the ability to 

drastically reduce the amount of synthetic insecticides used for sweet corn production.” 

Monsanto has proposed the use of MON 89034 as a sweet corn product to control certain 

lepidopteran insect pests in conjunction with no structured refuge. While sweet corn has a 

similar pest spectrum to field corn, agronomic practices differ between sweet corn and field 

corn. This makes pest management different between the two crops. As described in 

Monsanto’s submission (Head 2006): “Sweet corn is harvested approximately 18 to 23 days 

after silk emergence, compared to field corn in which the grain is allowed to mature and dry 

in the field. For sweet corn, the ears are harvested while still wet and placed in cold storage 

for fresh market corn or processed immediately. Shortly after harvest, corn stalks are 

typically destroyed in the field by disking, chopping or plowing. Previous work by Lynch et 

al. (1999), show that these harvest and post-harvest practices make it unlikely that any 

surviving/resistant larvae could survive, complete its development, and contribute any 

resistant allele to the next generation in sweet corn. Even if a larva was to survive, sweet 

corn farmers, including home gardeners, typically grow sweet corn in small plots along with 

many other vegetables that serve as alternative hosts for these polyphagous lepidopteran 

pests. Therefore, sufficient non-corn refuge should be present due to the typical practices of 

planting multiple host crops.” 

BPPD requested in a January 17, 2007 letter to Monsanto that the company provide 

additional (dose) data to support the sweet corn use. Monsanto responded to BPPD’s request 

for supplemental data on March 9, 2007. Monsanto provided data that compared the 

estimated Cry1A.105 and Cry2Ab2 protein levels in leaf tissues collected from MON 89034 

sweet corn varieties with field corn varieties (see Table 4 in Bogdanova, 2007; MRID# 

470794-02). Sweet corn data came from one site and field corn data came from five sites. 

The mean levels of the Cry1A.105 and Cry2Ab2 proteins were comparable between field and 

sweet corn MON 89034 hybrids. 

BPPD agrees with Monsanto that no structured refuge is needed in conjunction with the 

sweet corn use based on the destruction of potential resistant larvae through cultivation 

practices. 
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f) Popcorn Use of MON 89034 

Monsanto proposed to use the same IRM plan described for field corn with popcorn uses for 

MON 89034. Monsanto stated that there are approximately 291,000 acres of popcorn grown 

annually in the U.S., with Illinois, Indiana, Iowa, Nebraska, and Ohio accounting for 86% of 

the planted acres (Pike, 2003). Popcorn, like field corn, is allowed to mature and dry in the 

field, and the pest spectra are essentially identical in popcorn and field corn. 

Monsanto provided no additional dose and/or efficacy data to what was provided for field 

corn to support the use of MON 89034 on popcorn. Without these data, the popcorn use 

cannot be supported. 

g) Other Elements of IRM for MON 89034 

Monsanto proposed to have resistance monitoring, grower education and compliance 

monitoring as necessary parts of the IRM program. They proposed to implement a program 

similar to what is currently carried out for MON 810 and other single-gene Bt corn products. 

In particular, the educational and compliance assurance programs for MON 89034 would 

follow the structure established through consultations between EPA and the industry, and 

will involve working closely with NCGA and other interested stakeholders. 

Similarly, post-commercial resistance monitoring programs would be established as an 

extension of existing programs to track the susceptibility of the key lepidopteran corn pests to 

the Cry1A.105 and Cry2Ab2 proteins. In the monitoring program, insect populations would 

be collected and each protein will be tested separately, rather than a mixture of the two 

proteins, because resistance to one protein could be masked by the activity of the other. As 

part of this program, baseline susceptibility studies are planned for the Cry1A.105 protein 

against ECB (through Dr. Blair Siegfried at the University of Nebraska), and for the 

Cry1A.105 and Cry2Ab2 proteins against SWCB (through Dr. Qisheng Song at the 

University of Missouri), and CEW (through Bruce Lang of Custom Bio-Products). The 

baseline susceptibility of ECB to Cry2Ab2 has already been assessed over a two year period 

(see Appendix 4 - Siegfried and Spencer, 2001 in Monsanto’s submission, Head, 2006; 

MRID# 469514-30). In the case of CEW, baseline studies and annual monitoring have been 

conducted for Cry2Ab2 protein as part of the Bollgard II cotton IRM program, and the 

resulting data will be useful for MON 89034. In addition to the formal monitoring program, 

any unusual damage from lepidopteran pests will be monitored by the routine scouting of

corn fields and be reported to Monsanto or local extension agents. 

A remedial action plan has been developed and approved by EPA for MON 810 and other 

single-gene Bt corn products (EPA, 2001). This plan describes a series of actions to 

investigate suspected resistance, confirm actual resistance, and mitigate the resistant 

population. The basis of this plan also is appropriate for MON 89034. However, because 

MON 89034 contains both the Cry1A.105 and Cry2Ab2 proteins, this product has the 
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advantage of having a “built-in” mitigation program if resistance evolves to one of the Cry 

proteins but not the other. Therefore, Monsanto indicated that the remedial action plan should 

only be implemented for MON 89034 if a field population evolves resistance to both the 

CrylA.105 and Cry2Ab2 proteins. 

Monsanto’s proposed program for resistance monitoring, grower education and compliance 

monitoring as part of the MON 89034 IRM program was determined to be “acceptable." No 

Cry1A.105 baseline susceptibility studies have been conducted at the time of the registration 

application, but are planned by Monsanto. Monsanto has indicated that baseline 

susceptibility information for ECB to Cry2Ab2 has been collected over a two-year period 

(summarized in Monsanto’s submission). For each protein, a discriminatory concentration 

(diagnostic dose) will have to be determined for use in the annual resistance monitoring 

program. Annual reporting to the Agency of the results of the resistance monitoring, grower 

education, and compliance monitoring is needed (as is required for all other Bt PIPs). If there 

is confirmed resistance to either protein then it must be reported to the Agency (see FIFRA 

6(a) incident reporting requirements and the requirements as part of the Remedial Action 

plan). 

 

h) Conclusions for Initial Registration13

MON 89034 field corn uses in the Corn Belt

1) Pyramids can reduce the need for large refuges. Monsanto had originally proposed that a 

5% structured refuge, rather than the current 20% structured refuge, be used with the field 

corn uses of MON 89034. However, Monsanto’s initial data and modeling do not 

support a 5% structured refuge for MON 89034 for field corn uses in the Corn Belt. 

There are uncertainties in the dose determination for ECB, SWCB, CEW, FAW (SS and 

RS mortality), cross-resistance likelihood of Cry1A.105, Cry1Ac, and Cry1Fa and its 

impact on the durability of MON 89034, and limitations of the simulation modeling. The 

current 20% structured refuge requirement for field corn uses of MON 89034 in the Corn 

Belt will be maintained until such time as Monsanto can address these uncertainties. 

2) Monsanto relied on the Roush (1998) model to support the need for a 5% structured 

refuge rather than a 20% structured refuge in the Corn Belt. Roush’s model (1998; 

Figure 2) indicated that a 5% structured refuge is equal to or greater than a 20% 

structured refuge for a highly effective, high dose single-gene product when a two-gene 

product (MON 89034 in this case) achieves at least 95% control of susceptible 

homozygotes and 70% control of heterozygotes assuming there is no cross-resistance. 

The dose information provided by Monsanto is not sufficient to demonstrate that each 

protein will kill 95% of the homozygous susceptible insects and 70% of the 

 13 The assessment for the amendment to reduce lepidopteran refuge requirements modified these conclusions. (See 

section II. E. 2.)
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heterozygotes. To support a 5 % refuge Monsanto will have to further investigate 

whether MON 89034 consistently has high mortality of susceptible homozygotes (>95%) 

and whether the heterozygote mortality is at least 70% for MON 89034 against the target 

pests (for the Corn Belt – ECB and SWCB). The 1998 SAP suggested several ways to 

estimate mortality for less susceptible larvae (i.e. heterozygotes) (EPA 1998). These 

techniques included testing larger, later instar larvae that may be less susceptible 

3) Monsanto has demonstrated that Cry1A.105 and Cry2Ab2 have different modes of action 

and, therefore, a low likelihood of cross-resistance. Cry1A.105 and Cry2Ab2 would be 

suitable partners in a pyramided product. Monsanto has also shown that there is a low 

likelihood of cross-resistance between Cry1A.105 and Cry1Ab. Monsanto has previously 

demonstrated that there is a low likelihood of cross-resistance between Cry2Ab2 and 

Cry1Ac. 

4) However, Monsanto did not address the likelihood of cross-resistance of Cry1A.105, 

Cry1Ac, Cry1Fa, proteins already in existing Bt corn and Bt cotton products, and what 

impact such cross-resistance would have on the durability of MON 89034. Monsanto 

must provide additional information on cross-resistance of Cry1A.105 and Cry1Fa and 

Cry1Ac (including binding site models and use of resistant colonies) for the target pests 

and determine how such cross-resistance may impact the durability of MON 89034. The 

Cry1A.105 protein is a chimeric protein consisting of Domains I and II and the Cterminus of Cry1Ac and Domain III of Cry1Fa. It is important to address not only the 

likelihood of cross-resistance potential of Cry1A.105 and Cry1Ab and, similarly, 

Cry1A.105 and Cry2Ab2 (which was done by Monsanto), but also that of Cry1A.105 and 

Cry1Ac and Cry1Fa. 

5) Additional species-specific (e.g, ECB and SWCB for the Corn Belt), spatially-explicit, 

landscape modeling is recommended to explore the durability of MON 89034 versus 

single-protein Bt corn products. Modeling would need to consider the impact of other Bt

proteins in the landscape that may confer some cross-resistance (to Cry1A.105, in 

particular) and how such cross-resistance would impact the durability of MON 89034 in 

the Corn Belt (use of simulation modeling). This is analogous to the species-specific 

simulation modeling that EPA required Monsanto do to support the use of natural refuge 

(instead of a structured refuge) for management of H. virescens and H. zea to the Cry1Ac 

and Cry2Ab2 proteins expressed in Bollgard II cotton. 

6) MON 89034 field corn use in cotton-growing areas. A 20% non-Bt corn refuge for MON 

89034 in the southern cotton-growing areas would be sufficient to manage the risk of 

resistance evolution to Bt corn and Bt cotton products assuming there is no crossresistance. However, Monsanto did not sufficiently address the cross-resistance of 

Cry1A.105, Cry1Fa, and Cry1Ac in the cotton-growing landscape and how crossresistance may impact the durability of MON 89034. Should cross-resistance be of 

concern then the durability of MON 89034 in the southern cotton-growing areas might be 

compromised. Monsanto must address this potential in subsequent simulation modeling. 

(See item 4 above.) 
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7) Sweet corn. No structured refuge is needed in conjunction with the MON 89034 sweet 

corn use based on the destruction of potential resistant larvae through cultivation 

practices. Grower agreements (also known as stewardship agreements) will specify that 

growers must adhere to the following refuge requirements or, in the case of sweet corn, 

harvest practices, as described in the grower guide/product use guide and/or in 

supplements to the grower guide/product use guide: 

For MON 89034 sweet corn, growers are required to destroy any MON 89034 sweet corn 

stalks that remain in the field following harvest via rotary mowing, discing, or plowdown within one (1) month of harvest. 

8) Popcorn. Monsanto provided no additional dose and/or efficacy data to what was 

provided for field corn to support the use of MON 89034 on popcorn. Without these 

data, the popcorn use cannot be supported. 

9) Other Important Elements of the IRM Plan. Monsanto’s proposed program for resistance 

monitoring, grower education and compliance monitoring as part of the MON 89034 

IRM program is “acceptable." No Cry1A.105 baseline susceptibility studies have been 

conducted, but are planned by Monsanto. Monsanto has indicated that baseline 

susceptibility information for ECB to Cry2Ab2 has been collected over a two-year period 

(summarized in Monsanto’s submission). For each protein, a discriminatory 

concentration (diagnostic dose) will have to be determined for use in the annual 

resistance monitoring program. Annual reporting to the Agency of the results of the 

resistance monitoring, grower education, and compliance monitoring is needed (as is 

required for all other Bt PIPs). If there is confirmed resistance to either protein then it 

must be reported to the Agency (see FIFRA 6(a) incident reporting requirements and the 

requirements as part of the Remedial Action plan). 

i) Insect Resistance Management Plan for MON 89034 X MON 88017 Bt Corn 

MON 89034 x MON 88017 expresses the Cry1A.105, Cry2Ab2, and Cry3Bb1 Bt toxins and 

is targeted against lepidopteran corn pests including European corn borer (ECB), 

southwestern corn borer (SWCB), corn earworm (CEW), and fall armyworm (FAW) as well 

as the coleopteran corn rootworm sp. pest complex (CRW). MON 89034 (Cry1A.105 and 

Cry2Ab2) provides activity against the lepidopteran corn stalk and ear insects while MON 

88017 (Cry3Bb1) is active against root-feeding CRW. The product was created by 

conventional breeding in which the previously-registered MON 88017 (EPA Reg. No. 524-

551) was crossed with MON 89034 (EPA Reg. No. 524-LTL). The Cry3Bb1 toxin in MON 

88017 is the same as expressed by MON 863 corn (Yieldgard Rootworm, EPA Reg. No. 525-

528), which was registered by Monsanto for the 2003 growing season. 

1) Monsanto has provided information to demonstrate that the dose of MON 89034 x MON 

88017 against the major target pests should be comparable to the dose of the MON 89034 
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and MON 88017 isolines. Therefore, the IRM considerations (dose, refuge, cross 

resistance) for MON 89034 and MON 88017 are applicable to the stacked MON 89034 x 

MON 88017 product. 

2) Monsanto has proposed a 5% lepidopteran refuge as part of the “Separate Refuge” option 

for MON 89034 x MON 88017 corn. Due to uncertainties in the review of the MON 

89034 IRM plan (see BPPD 2007a), a 5% refuge cannot be supported at the present time. 

Instead, BPPD recommends that the separate refuge option include a 20% lepidopteran 

refuge (as has been required for other Bt corn products). However, BPPD notes that a 

20% refuge can be supported for MON 89034 x MON 88017 in cotton-growing regions 

in southeastern U.S. where a 50% refuge has been previously required. 

3) Monsanto’s proposal for a combined refuge (covering both coleopteran and lepidopteran 

pests) is acceptable. This option calls for a 20% refuge throughout the U.S. (as described 

in #2 above, a 20% refuge can be supported in southern cotton-growing regions). 

4) The other aspects of Monsanto’s IRM plan for MON 89034 x MON 88017 including 

resistance monitoring, remedial action plans, grower education, compliance, and annual 

reporting are acceptable. Resistance monitoring (sampling, bioassays, and data 

reporting) and remedial action should be conducted under the terms and conditions of 

registration for MON 89034 and MON 88017. 

2. Amendment to Reduce Lepidopteran Refuge to 5%. 

After the registration of MON 89034 was granted (with a 20% lepidopteran refuge in the Corn 

Belt, as described in the preceding section), Monsanto submitted an amendment with supporting 

data to request a reduction in refuge to 5% in the Corn Belt. This section contains BPPD’s 

assessment of this proposal (based on the review contained in BPPD, 2008). 

As part of the IRM proposal for MON 89034 corn, Monsanto proposed a 5% lepidopteran 

structured refuge for non-cotton growing regions instead of the 20% refuge that has been 

required for all other Bt corn registrations. Monsanto reasoned that the combination of two 

toxins targeting lepidopteran corn pests with no cross resistance allowed for a reduced refuge 

with little risk of resistance. As described in section 1 above, BPPD’s review of the IRM 

proposal (BPPD, 2007) agreed with much of Monsanto’s justification but determined that there 

were a number of uncertainties in the request for lower refuge. Specifically, there were three 

areas of concern: (1) Cry1A.105 and Cry2Ab2 dose determination for the major target pests 

(ECB, CEW, SWCB, and FAW); (2) cross resistance potential between Cry1A.105 and Cry1F 

and Cry1Ac (toxins expressed in previously-registered PIPs); and (3) species-specific (e.g, ECB 

and SWCB for the Corn Belt), spatially-explicit, landscape modeling to explore the durability of 

MON 89034 versus single-protein Bt corn products. Given the uncertainty of the reduced refuge 

request, EPA registered MON 89034 with a 20% structured refuge requirement, similar to other 
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Bt corn products. Separately, EPA did agree with Monsanto’s request to reduce refuge in cottongrowing areas from 50% to 20% (see discussion in section 1 above and in BPPD, 2007). As a 

condition of registration, Monsanto was required to address cross resistance in existing Bt corn 

and Bt cotton products for Cry1A.105, Cry1Fa and Cry1Ac. 

Monsanto subsequently provided materials to address these three areas of uncertainty as part of a 

new amendment request for a reduced 5% refuge for non-cotton regions. The response, 

including a discussion of cross resistance and a new model, is included in a study titled 

“Assessment of the Impact of MON 89034 Introduction on Bt Resistance Development in 

European and Southwestern Corn Borer” (MRID# 474748-01). 

a) Monsanto’s Proposed Amendment to Support a 5% Refuge for MON 89034

Monsanto’s proposal for a 5% refuge with MON 89034 included two major components: (1) a 

discussion of the cross resistance potential between the toxins in MON 89034 and (2) a 

deterministic model to simulate a 5% refuge and the risk of resistance for ECB and SWCB. 

Each of these sections is described and reviewed individually below. 

In lieu of submitting new dose determination data for Cry2Ab2 and Cry1A.105 for the major 

target pests, Monsanto has used the existing dose information (submitted for the original 

registration) in the new simulation model. Therefore, Monsanto’s response to the dose 

determination uncertainties (detailed in BPPD 2007 and section 1 above) will be discussed and 

reviewed in the modeling portion (section ii.) below. 

i) Cross Resistance Potential 

MON 89034 contains both Cry1A.105 and Cry2Ab2, which target the same lepidopteran corn 

pest complex. The Cry1A.105 toxin is a “chimeric” protein containing domains I and II and the 

C-terminal from Cry1Ac and domain III from Cry1Fa while the Cry2Ab2 protein is the same as 

that currently expressed in Monsanto’s Bollgard II cotton. Monsanto has sufficiently 

demonstrated that the cross resistance potential between these two proteins should be low, 

primarily due to differing modes of action (see discussion in BPPD 2007). In evaluating new 

PIP traits, the landscape of previously registered toxins in the same crop must be taken into 

account. In addition, for corn PIPs, cotton must also be considered because one of the key target 

pests, corn earworm (also referred to as cotton bollworm, CBW, when a pest on cotton), is a pest 

of both crops. As a condition of registration, Monsanto was required to address cross resistance 

in existing Bt corn and Bt cotton products for Cry1A.105, Cry1Fa and Cry1Ac. 

Monsanto’s amendment submission for MON 89034 contained a discussion of cross resistance 

including an analysis of previous studies as well as a summary of recently developed data. 

Analysis of existing data was conducted for four toxin combinations: 1) Cry1Ab vs. Cry1Ac; 2) 

Cry1F vs. Cry1Ab and Cry1Ac; 3) Cry2Ab2 vs. Cry1 proteins; and 4) Cry1A.105 vs. Cry1Ab 
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and Cry1Ac. New data were presented for comparisons between Cry1A.105 and Cry2Ab2 vs. 

Cry1F. 

Cry1Ab vs. Cry1Ac: Based on a literature review of binding studies with numerous lepidopteran 

species, Cry1Ac is known to have strong cross resistance with Cry1Ab. Both toxins share a high 

affinity binding site in ECB, CEW/CBW, SWCB, FAW, and others (references cited in MRID# 

474748-01). 

Cry1F vs. Cry1Ab and Cry1Ac: Cry1F also shares a binding site with Cry1Ab/Cry1Ac, though 

the level of cross resistance between Cry1F and Cry1A is not as strong as Cry1Ab vs. Cry1Ac. 

ECB resistant to Cry1Ab have been shown to be partially resistant to Cry1F although Cry1F 

resistant ECB were not cross resistant to Cry1Ab and only slightly resistant to Cry1Ac. Similar 

trends have also been shown with tobacco budworm (Heliothis virescens, TBW) (references 

cited in MRID# 474748-01). Overall, Cry1F can be considered partially cross resistant to 

Cry1Ab and Cry1Ac. The availability of binding sites may explain the partial cross resistance: 

Cry1Ab and Cry1Ac could have more different sites to bind with than Cry1F so that resistance to 

Cry1F still allows for some binding of Cry1Ab or Cry1Ac. 

Cry2Ab vs. Cry1 proteins: A literature review suggests that Cry2Ab has no cross resistance 

potential with any of the currently registered Cry1 proteins including Cry1Ab and Cry1Ac. 

Studies have been conducted with numerous cotton pests including CEW, TBW, pink bollworm 

(Pectinophora gossypiella, PBW), and Helicoverpa armigera that revealed no shared binding 

sites between Cry2A and Cry1Ab or Cry1Ac proteins. Additional studies with Cry1Ac-resistant 

TBW, CEW/CBW, and PBW found no cross resistance with Cry2Ab (references cited in MRID# 

474748-01). Previously submitted data by Monsanto for MON 89034 (Head 2006; reviewed in 

BPPD 2007) demonstrated that Cry1Ab-resistant ECB were not found to be cross resistant with 

Cry2Ab while Cry2Ab2-resistant H. armigera were not cross resistant with Cry1A.105 or 

Cry1Ac. 

Cry1A.105 vs. Cry1Ab and Cry1Ac: For Cry1Ab, a previously submitted binding study with 

ECB (Head 2006; reviewed in BPPD 2007) showed that the protein has a distinct binding site 

from Cry1A.105. This was confirmed by studies with Cry1Ab-resistant ECB and sugarcane 

borer (Diatraea saccharalis, SCB) that showed no cross resistance with Cry1A.105. Monsanto 

argues that due to similar characteristics between Cry1Ab and Cry1Ac (i.e. mode of action), it is 

reasonable to assume that Cry1Ac should not be cross resistant with Cry1A.105. However, no 

binding studies or experiments with resistant colonies were described to verify that assumption. 

Cry1A.105 and Cry2Ab2 vs. Cry1F: New data were cited by Monsanto (Schlenz et al. 2008) to 

assess the cross resistance potential between Cry1A.105/Cry2Ab2 and Cry1F using Cry1Fresistant ECB and FAW colonies. Artificial diet bioassays were used to test Cry1A.105, 

Cry2Ab2, and control groups against ECB and FAW colonies previously selected for high-level 

Cry1F resistance as well as unselected control colonies. A range of five concentrations was used 

and the test was conducted over a seven day period to determine growth inhibition (GI50) for 
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each colony. The results showed that, as expected, Cry1F-resistant ECB and FAW were not 

cross resistant with Cry2Ab2 -- the GI50 resistance ratios (Cry1F-resistant : Cry1F-susceptible) 

were 1.4 for ECB and 0.11 for FAW. With Cry1A.105, the GI50 resistance ratios were > 3.9 for 

ECB and 7.0 for FAW, indicating low level cross resistance. 

Table 7: Cross resistance potential of MON 89034 (Cry1A.105 and Cry2Ab2) with previously 

registered Bt corn toxins. 

Bt toxins in MON 89034 

Existing Bt toxins Cry1A.105 Cry2Ab2 

Cry1Ab No cross resistance (ECB, 

SCB) 

No cross resistance (ECB) 

Cry1Ac Unlikely cross resistance, but 

unverified experimentally 

No cross resistance (TBW, 

PBW, CEW/CBW) 

Cry1F Low level cross resistance 

(ECB, FAW) 

No cross resistance (ECB, FAW)

BPPD Review - Cross Resistance

BPPD agrees with Monsanto’s characterization of the cross resistance potential for the 

Cry1A.105 and Cry2Ab2 toxins with (1) each other (previously demonstrated in Head 2006), (2) 

Cry1F, and (3) Cry1Ab. Binding and resistant colony work conducted by Monsanto and other 

researchers clearly show that no cross resistance can be expected between Cry1A.105, Cry2Ab2 

and Cry1Ab (see Table 7. above). New data referenced in Monsanto’s amendment request also 

experimentally demonstrate the cross resistance potential between Cry1F and Cry2Ab2 (no cross 

resistance) and Cry1A.105 (low cross resistance). 

Nonetheless, BPPD still has reservations about Cry1Ac. While Monsanto has made the case that 

Cry1Ac should be expected to behave like Cry1Ab due to a similar mode of action, no 

experimental data (i.e. binding studies or bioassays with resistant insect colonies) were provided 

either in the original MON 89034 IRM submission (Head 2006) or the follow-up amendment 

request (MRID# 474748-01). BPPD notes that Cry1A.105 (a chimeric protein) contains domains 

I and II and the C-terminal from Cry1Ac. Cross-resistance could result when proteins share key 

structural features, which allows one resistance mechanism to confer resistance to more than one 

protein (Tabashnik, 1994; Gould et al., 1995). 

BPPD recognizes that at the present time there are no registered Bt corn products containing 

Cry1Ac. Therefore, exposure to ECB and SWCB to Cry1Ac is unlikely, as neither is known as a 

cotton pest. FAW may occasionally feed on cotton, but favors corn and is also unlikely to have 

much exposure to Cry1Ac. On the other hand, successive generations of CEW may feed on both 

corn and cotton during the same growing season. This could result in a potential “double” 
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exposure to Bt cotton (including Cry1Ab) and Bt corn (including Cry1A.105) and increased 

selection pressure for resistance, particularly if there is a risk of cross resistance. 

Given that Monsanto has proposed to substantially reduce refuge for MON 89034 from 20% to 

5%, cross resistance is an important consideration even for Cry1Ac. Although improbable, 

BPPD cannot rule out that a CEW/CBW population could develop Cry1Ac resistance in cotton 

and then encounter MON 89034 corn. [Tabashnik et al. (2008) have argued that Cry1Ac 

resistance has already evolved in CBW in the south, although this conclusion has been disputed 

(Moar et al. 2008).] Should there be a degree of cross resistance between Cry1Ac and 

Cry1A.105, MON 89034 might functionally have only Cry2Ab2 remaining as an effective toxin 

against CEW. With a reduced refuge (5%), selection pressure could be increased for resistance 

to MON 89034 and Cry2Ab2 (which also is expressed in Bollgard II cotton). So that BPPD can 

fully assess the cross resistance potential of Cry1A.105 with Cry1Ac in CEW/CBW, it is 

recommended that Monsanto provide additional information either experimentally (e.g., binding 

studies or with resistant colonies) or using another analysis. Alternatively, Monsanto could 

revise the CEW model submitted with the original MON 89034 IRM plan (Head 2006) to 

support 20% refuge in cotton-growing regions. This model simulated CEW resistance to MON 

89034 and assumed complete cross resistance between Cry1A.105 and Cry1Ac; the model could 

be adapted to evaluate a 5% refuge in the Corn Belt with similar assumptions. 

ii) Modeling 

As part of the review of Monsanto’s initial IRM plan for MON 89034, BPPD identified the need 

for additional species-specific (e.g, ECB and SWCB for the Corn Belt), spatially-explicit, 

landscape modeling to explore the durability of MON 89034 versus single-protein Bt corn 

products (BPPD 2007). Previously, Monsanto had cited the modeling work of Roush (1998) to 

demonstrate that a 5% refuge was justified with a two toxin pyramided product. Roush’s model 

made a number of key assumptions, particularly in terms of the toxin expression level in 

pyramided product. For homozygote susceptible insects, the model assumed 95% mortality and 

70% mortality for heterozygotes (with one resistance allele) for each toxin. However, the dose 

information provided by Monsanto for MON 89034 was not sufficient to demonstrate that each 

protein would kill 95% of the homozygous susceptible insects and 70% of the heterozygotes (see 

BPPD 2007). BPPD recommended that Monsanto further characterize the dose expression for 

the MON 89034 toxins for the major target pests of the Corn Belt (ECB and SWCB). Given the 

dose uncertainties, BPPD could not at the time of registration support the use of Roush’s model 

to justify a lower 5% refuge for MON 89034 (BPPD 2007). 

Rather than re-run dose studies for Cry1A.105 or Cry2Ab2, Monsanto created a deterministic 

model for ECB and SWCB using dose mortality estimates consistent with the previously 

conducted studies. The model (Gustafson and Head 2008; contained in MRID# 474748-01) 

included the toxins from other registered Bt corn products (Cry1Ab, Cry1F) and had a number of 

assumptions and parameters: 
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 Dose mortality for ECB: 99.9% for Cry1 (Cry1Ab, Cry1F, Cry1A.105) and Cry2Ab2 

toxins (one mortality scenario was modeled); 

 Dose mortality for SWCB: 99 - 99.5% for Cry1 and 85 - 95% for Cry2Ab2 (six dose 

mortality scenarios were modeled); 

 Complete resistance to Cry2Ab2 and Cry1A.105 (i.e. survival probability of heterozygote 

resistant individuals = 1) with no fitness costs; 

 Heterozygotes (i.e. with one resistance allele) survival probability is twice that for 

homozygote susceptible insects; 

 Three cross resistance scenarios: 1) Cry1A.105 and Cry1Ab fully cross resistant (but not 

Cry1F) (the “base case” scenario); 2) Cry1A.105 and Cry1F fully cross resistant (but not 

Cry1Ab) (alternate “base case” scenario), and 3) Cry1A.105, Cry1Ab, and Cry1F all fully 

cross resistant (worst case scenario); 

 All resistance alleles (Cry1, Cry1A.105, and Cry2Ab2) have initial frequencies of 0.005. 

Cry1Ab and Cry1F are modeled as one output (i.e. estimated time to resistance for 

Yieldgard/Herculex); 

 MON 89034 was assumed to have a refuge of 5%; other single gene products (Yieldgard 

and Herculex) were assumed to have 20% refuge; 

 ECB and SWCB have no natural refuge (i.e. wild hosts or other cultivated crops that 

could serve as a source of susceptible insects) and have two generations per year on corn; 

 A range of market share adoption values for MON 89034 and other products (Herculex 

and Yieldgard) were included in the model simulations. MKT 1 = 100% MON 89034; 

MKT 2 = 50% MON 89034, 25% MON 810, 25% TC1507; MKT 3 = 0% MON 89034, 

50% MON 810, 50% TC1507. 

Most of the assumptions above were conservative estimates, with the possible exception of the 

dose mortality parameters for SWCB (see discussion in the BPPD review section below). 

Simulations were run with both ECB and SWCB to estimate the time to resistance (in years; up 

to a maximum of 30 years) and resistance allele frequency for each of the three cross resistance 

scenarios described above. Within each cross resistance scenario, model runs were conducted 

for three different market adoption contingencies of MON 89034, MON 810 (Cry1Ab 

Yieldgard) and TC1507 (Cry1F Herculex). 

ECB Results 

For ECB, the results of the model runs were relatively consistent among the different cross 

resistance and market adoption scenarios. In almost all cases, the durability of the MON 89034 

toxins (Cry1A.105 and Cry2Ab2; assuming a 5% refuge) exceeded the 30 year time frame of the 

model. Only in the “worst case” cross resistance scenario (i.e., all three toxins cross resistant) 

was the durability of Cry1A.105 less than 30 years (29 years) for ECB -- Cry2Ab2 remained 

effective in all model simulations (> 30 years). For the other Cry1 toxins (Cry1Ab and Cry1F) 

that are expressed in other Bt corn products, resistance developed in less than 30 years for some 

of the cross resistance and market adoption scenarios. In the “base case” (Cry1Ab and 
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Cry1A.105 cross resistant), the durability of Cry1Ab/Cry1F lasted 26 years (0% MON 89034, 

50% MON 810, 50% TC1507) and 29 years (50% MON 89034, 25% MON 810, 25% TC1507). 

However, for the alternate base case (Cry1F and Cry1A.105 cross resistance), resistance to 

Cry1Ab/Cry1F did not evolve within 30 years. In the worst case scenario (all three toxins cross 

resistant), resistance to Cry1Ab/Cry1F developed in 29 years. 

SWCB Results 

For SWCB, more model simulations were run to account for a range of dose mortalities. 

Overall, durability of the traits was affected by the dose mortality scenarios -- the simulations 

with lower dose mortality frequently resulted in fewer years to resistance in Cry1A.105 and 

Cry1F than those with higher dose mortalities. As with ECB, Cry2Ab2 remained durable (> 30 

years) in all but one of the simulations regardless of the cross resistance or market adoption 

scenario. 

For the “base case” cross resistance scenario, the time to resistance was lowest in the market 

adoption scheme (MKT 3) without MON 89034 (50% MON 810, 50% TC1507) ranging from 17 

years (lower dose mortalities for Cry1 and Cry2Ab2 toxins) to 20.5 years (higher dose 

mortalities). Once MON 89034 was added to the model (MKT 1 and 2), the time to resistance 

with the Cry1 toxins increased by 2 -2.5 years for all simulations. Cry1A.105 and Cry2Ab2 did 

not evolve resistance in any of the model runs for MKT 2, although there were two instances 

with MKT 1 (100% MON 89034) in which resistance evolved within 30 years. In both of these 

cases, lower dose mortality values for SWCB (85% for Cry2Ab2; 99% for Cry1A.105) were 

included in the model. 

Time to resistance in the “alternate base case” (Cry1F and Cry1A.105 cross resistant) was > 30 

years in almost all cases. Only in the simulation that incorporated the lowest dose mortality 

values (85% for Cry2Ab2 and 99% for Cry1A.105) did resistance evolve to one of the toxins 

(28.5 years for Cry1A.105). 

In the “worst case” (Cry1Ab, Cry1F and Cry1A.105 are all cross resistant), resistance developed 

in all scenarios for both the Cry1 toxins and Cry1A.105. Conversely, Cry2Ab2 remained 

durable (> 30 years) for all of the simulations. Time to resistance in the Cry1 and Cry1A.105 

toxins was lowest (17 years) in the model run using the lower SWCB dose mortality values (85% 

for Cry2Ab2 and 99% for Cry1A.105). Resistance also evolved for case with the higher dose 

mortality values, ranging up to 22 years for each toxin. A truncated summary of the results for 

all of the model simulations is contained in Table 8. below -- the complete results of the 

modeling are detailed in Tables 5 and 6 in Monsanto’s submission (MRID# 474748-01). 
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Table 8: Results of Monsanto’s model simulations of MON 89034 (5% refuge), MON 810, 

TC1507 (20% refuge) expressed in years to resistance (30 year maximum). Derived from data 

reported in MRID# 474748-01. 

Cross resistance scenario 

Base case1 Pest 

MKT 1 MKT 2 MKT 3 

Alt. base 

case2 Worst case3 

ECB 

Cry1A.105 

Cry2Ab2 

Cry1Ab/Cry1F 

>30 

>30 

N/A 

>30 

>30 

29 

N/A 

N/A 

26 

>30 

>30 

>30 

29 

>30 

29 

SWCB 

Cry1A.105 

Cry2Ab2 

Cry1Ab/Cry1F 

22.5 - >30 

25 - >30 

N/A 

>30 

>30 

19 - 23 

N/A 

N/A 

17 - 20.5 

28.5 - >30 

>30 

>30 

17 - 22 

>30 

17 – 22 

1

 Base case = Cry1Ab and Cry1A.105 cross resistant; three different marketing scenarios included (Mkt 1 = 100% 

MON 89034, 0% MON 810/TC1507; Mkt 2 = 50% MON 89034, 25/25% MON 810/TC1507; Mkt 3 = 0% MON 

89034, 50/50% MON 810/TC1507). 

2

 Alt. base case = Cry1F and Cry1A.105 cross resistant (only Mkt 2 simulated). 

3

 Worst case = Cry1A.105, Cry1Ab, and Cry1F all fully cross resistant (only Mkt 2 simulated).

Based on the model work, Monsanto concluded that the durability of the MON 89034 proteins 

(Cry1A.105 and Cry2Ab2) will remain strong for both ECB and SWCB. With a 5% refuge, 

Monsanto predicted that MON 89034 will have at least 22 years durability even under the “worst 

case” model assumptions. The durability of Cry2Ab2 in the model was particularly robust in 

almost all simulations for ECB and SWCB (only one simulation predicted less than 30 years 

durability). Resistance to Cry1A.105 was also rare in most simulations, although the “worst 

case” modeling (assuming complete cross resistance with Cry1Ab and Cry1F) showed resistance 

developing in less than 30 years. Monsanto also noted that in the simulations with different 

market adoption scenarios, the addition of MON 89034 increased the time to resistance for the 

previously registered Cry1 toxins (Cry1Ab and Cry1F). 

BPPD Review - Modeling

BPPD agrees with Monsanto’s overall conclusions that the model simulations demonstrate the 

effectiveness in delaying resistance of MON 89034 and provide support for the use of a 5% 

refuge in the Corn Belt. However, BPPD notes that some of the parameters and assumptions of 

the model could be revised to improve and expand the overall analysis. 

For ECB, the model clearly predicts that resistance is unlikely to evolve to Cry1A.105, Cry2Ab2, 

or the previously-registered Cry1 toxins. Even under the worst case scenario that assumed 

complete cross resistance, the durability of all toxins was at least 29 years. Presumably, a large 

reason for this is the high dose mortality of the MON 89034 toxins against ECB. Previous 

mortality studies submitted by Monsanto (reviewed in BPPD 2007) showed that the Cry1A.105 
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and Cry2Ab2 proteins in MON 89034 each provide essentially 100% control of ECB (Monsanto 

assumed 99.9% mortality for each toxin in the model). 

For SWCB, the model predictions were more varied, largely due to the different simulations run 

with the range of dose mortality assumptions. Not surprisingly, the simulations that were run 

with the lower mortality estimates (i.e. 85% for Cry2Ab and/or 99.0% for Cry1) resulted in less 

time to resistance than those using the higher dose values. In the worst case simulations with the 

lower dose estimates, SWCB resistance evolved in 17 years to both Cry1A.105 and 

Cry1Ab/Cry1F while with the higher doses resistance took 21 or 22 years to develop. As with 

ECB, Cry2Ab2 remained durable (>30 years) for almost all of the simulations. 

A number of factors appeared to influence the model results. BPPD agrees with Monsanto that 

the addition of MON 89034 in the simulations testing various market adoption scenarios delayed 

resistance in the other previously-registered Cry1 toxins. Likely, these results were due to less 

selection pressure on each individual toxin because of a diverse mosaic of toxins in the 

landscape. Cross resistance was also an important variable. Monsanto’s “base case” for cross 

resistance assumed cross resistance between Cry1Ab and Cry1A.105. This resulted in resistance 

always developing in Cry1Ab/Cry1F (i.e. within 30 years), although Cry1A.105 and Cry2Ab2 

durability remained strong. On the other hand, when cross resistance between Cry1A.105 and 

Cry1F was assumed, resistance rarely developed in either the MON 89034 toxins or the existing 

Cry1 toxins. In the worst case scenario (all three toxins cross resistant), the durability of 

Cry1A.105 to SWCB was clearly impacted relative to the other cross resistance simulations. 

Conversely, Cry2Ab remained durable in almost all cases regardless of the varying assumptions 

and scenarios included in the model. Since Cry2Ab is not cross resistant to the Cry1 toxins, this 

result was not unexpected. 

BPPD generally agrees with Monsanto that conservative assumptions were used in the model. 

However, BPPD notes that several of the parameters could have been expanded or have included 

an additional degree of conservatism or additional refinement to improve the model analysis. 

For example, Monsanto’s simulations assumed a 5% refuge for MON 89034 (while maintaining 

the 20% refuge for the other Bt toxins). Although MON 89034 is currently registered with a 

requirement for a 20% refuge, simulations were not run with the larger refuge size. Separate 

simulations with 5% and 20% MON 89034 refuges would have been useful for comparative 

purposes. To illustrate using the SWCB “base case” (with the three different marketing adoption 

cases), with no MON 89034 adoption resistance to the Cry1 toxins occurred in 17 - 20.5 years. 

When MON 89034 with a 5% refuge was included, the time to Cry1 resistance was 19 - 23 years 

-- indicating that the addition of MON 89034 provides some delay in resistance development (2 - 

2.5 years). It would have been interesting to observe the impact of adoption of MON 89034 with 

a 20% refuge on Cry1 resistance. In all likelihood, the time to resistance would be increased, 

although the magnitude of such an increase is unknown. Had the difference been small, it could 

be argued that there is little value gained in having a 20% refuge versus a 5% refuge. 
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The model time frame (maximum 30 years) was another limiting parameter. Many of the 

simulations resulted in no resistance within the 30 year time period of the model, so it was 

difficult to discern the effects of certain variables (i.e. cross resistance, market adoption, dose 

mortality) between model runs. Had the time horizon been extended (e.g. to 50 years), 

differences between the various model scenarios may have been apparent. 

For the SWCB simulations, Monsanto used dose mortality range of 85-95% for Cry2Ab2 and 

99-99.5% for Cry1 toxins. Based on the dose data submitted for the registration of MON 89034 

(reviewed in BPPD 2007), BPPD believes these estimates to be somewhat high. For example, 

dose data for Cry2Ab2 and SWCB suggested a mortality range of 80-90%. The Cry1A.105 

protein in MON 89034 provided approximately 95% control in mortality assays, though the other 

registered Cry1 proteins (Cry1Ab and Cry1F) may provide closer to 99% of SWCB. Had the 

model simulations been run with these more conservative dose estimates, it is likely the time to 

resistance would have been reduced in some scenarios. The extent of this effect is unknown, 

although BPPD notes that the differences between the lower Cry2Ab2 dose (85%) and the 

highest dose (95%) in the range appeared to be negligible in the model runs (i.e. no differences 

in years to resistance). 

iii. BPPD Review - Overall Proposal to Reduce Refuge

Taken together, Monsanto’s cross resistance and modeling work provides justification for 

reducing the MON 89034 structured refuge requirement in the Corn Belt from 20% to 5% non-Bt

corn. Key elements of support include a lack of cross resistance between Cry2Ab2 and Cry1 

proteins and model simulations which demonstrate strong durability of Cry1A.105 and Cry2Ab2 

under a variety of dose, market adoption, and cross resistance scenarios. Reducing the refuge to 

5% is unlikely to increase the selection pressure for resistance in either MON 89034 or the other 

previously-registered Cry1Ab or Cry1F corn hybrids. 

Despite a good case for a refuge reduction, BPPD notes that there are still some limitations and 

uncertainties in the analysis that could be addressed to provide additional support for the 

proposal. These areas include: 

 Cross resistance between Cry1Ac and Cry1A.105. Cry1Ac is registered in Bt cotton 

products and the chimeric protein Cry1A.105 has two Cry1Ac domains. CEW feed on 

both corn and cotton and successive generations may have exposure to both Cry1A.105 

and Cry1Ac during the same growing season; 

 No model simulations were conducted to compare 5% vs. 20% refuge for MON 89034; 

the model assumed a 5% refuge for MON 89034; 

 The model time horizon was limited to 30 years. Many of the model runs did not evolve 

resistance during this time precluding comparisons between some of the scenarios; 

 SWCB model simulations included dose mortality estimates somewhat higher than those 

suggested by previously-submitted data. For Cry2Ab2, mortality ranged from 80 to 90% 

in dose testing submitted for MON 89034 (instead of 85-95% used in the model). 
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Cry1A.105 caused 95% mortality in submitted dose studies, though a range of 99-99.5% 

was used in the model. 

As a condition of registration of MON 89034, Monsanto was required to address cross resistance 

in existing Bt corn and Bt cotton products for Cry1A.105, Cry1Fa and Cry1Ac. Monsanto has 

sufficiently addressed cross resistance for Cry1A.105 and Cry1Fa, but there are lingering 

questions regarding Cry1Ac and Cry1A.105. The amendment submission included only a 

circumstantial discussion of Cry1Ac cross resistance with an assumption that the protein will 

behave similarly to Cry1Ab. However, since Cry1A.105 contains domains I and II and the Cterminal from Cry1Ac, BPPD is still concerned about the potential for cross resistance. As such, 

BPPD recommends additional work (as described in the cross resistance section above) to satisfy 

the condition of registration. Should additional cross resistance work (as previously described) 

demonstrate little or no cross resistance potential between Cry1A.105 and Cry1Ac, further 

support could be provided for the use of a 5% refuge in the Corn Belt. 

In terms of resistance risk for MON 89034, cross resistance between Cry1Ac and Cry1A.105 is 

an issue primarily for CEW. This insect is known to feed on both corn and cotton during the 

same growing season and could be exposed to Cry1A.105 (in corn) and then Cry1Ac (in 

Bollgard cotton) later in the growing season. Theoretically, CEW could develop resistance to 

Cry1Ac due to exposure in cotton -- should there be a degree of cross resistance between Cry1Ac 

and Cry1A.105, MON 89034 could functionally have only Cry2Ab2 remaining as an effective 

toxin against CEW. With a reduced refuge (5%), selection pressure could be increased for 

resistance to MON 89034 and Cry2Ab2 (which also is expressed in Bollgard II cotton). While 

these are legitimate concerns (and reason for additional analysis), BPPD notes that there are 

several mitigating factors that reduce the overall resistance risk for CEW and MON 89034. 

First, CEW is generally a lesser pest in the Corn Belt than ECB (and in some areas SWCB), 

primarily due to poor overwintering capability in much of the Corn Belt (i.e., north of Virginia, 

Tennessee, and Missouri). Therefore, selection pressure for resistance will likely be less for 

CEW than ECB which does overwinter in the Corn Belt. On the other hand, in cotton-growing 

regions south of the Corn Belt where CEW can overwinter, conditions for resistance 

development may be more probable. In these areas, a 20% refuge (approved with the initial 

registration of MON 89034) will still be required. Along these lines, in Monsanto’s original 

MON 89034 IRM submission, modeling was conducted to support the use of a 20% refuge for 

CEW in southern cotton-growing regions (see discussion in BPPD 2007). 

A second mitigating factor is that CEW is a highly polyphagous insect and is known to feed on a 

wide variety of plants including weeds, wild hosts, and other cultivated crops (unlike ECB and 

SWCB which feed primarily on corn). Analysis conducted for Bollgard II cotton determined 

that a natural refuge is present for CEW (CBW) in cotton growing areas in the southeastern U.S. 

(see BPPD 2004 and 2006). It is likely that in the Corn Belt, there is also at least some degree of 

natural refuge that could supplement a 5% structured refuge to help reduce the overall selection 

pressure on CEW and MON 89034. BPPD emphasizes that natural refuge for CEW has been 
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quantified only in cotton-growing regions and that host utilization patterns in the Corn Belt are 

speculative. 

The other modeling parameter uncertainties detailed above are relatively minor, though a more 

expanded model analysis could have provided stronger support for the proposal. Separate model 

runs with 5% and 20% MON 89034 refuges would have been useful to compare potential 

differences in times to resistance. Although since most of the simulations did not result in 

resistance within 30 years, any differences would have been difficult to detect. Expanding the 

time horizon of the model (for example, from 30 years to 60 years) possibly could have fleshed 

out variation between model scenarios and provided a more thorough basis for comparison. 

Finally, BPPD would have preferred if Monsanto had used the more conservative estimates of 

SWCB dose mortality (based on the MON 89034 dose data), though the impact on the model 

output would likely have been relatively small. 

MON 89034 was registered as an expiring registration (expiration date: September 30, 2010) 

and BPPD recommends reevaluating 5% refuge if warranted by cross resistance data or other 

information during this interim period. 

F. BENEFITS AND PUBLIC INTEREST FINDING 

To grant a conditional registration under Section 3(c)(7)(C) of the Federal Insecticide, Fungicide, 

and Rodenticide Act (FIFRA), EPA must determine that such conditional registration will, inter 

alia, be in the public interest. EPA determines whether conditional registration of a pesticide is 

in the public interest in accordance with the criteria set forth at 51 Fed. Reg. 7628 (Conditional 

Registration of New Pesticides, March 5, 1986). There is a presumption that registration of a 

pesticide is in the public interest if one of the following criteria are met: (i) the use is for a minor 

crop; (ii) the use is a replacement for another pesticide that is of continuing concern to EPA; (iii) 

the use is one for which an emergency exemption under FIFRA Section 18 has been granted (the 

basis for the exemption was lack of a registered alternative product); or (iv) the use is against a 

pest of public health significance. Notwithstanding whether a registration of a pesticide may be 

presumed to be in the public interest, EPA may determine that such a registration is in the public 

interest on the basis of one of the following criteria: (i) there is a need for the new chemical that 

is not being met by currently registered pesticides; (ii) the new pesticide is comparatively less 

risky to health or the environment than currently registered pesticides; or (iii) the benefits 

(including economic benefits) from the use of the new active ingredient exceed those of 

alternative registered pesticides and other available non-chemical techniques. 

MON 89034 and MON 89034 x MON 88017 do not meet any of the criteria for a presumption of 

public interest; however, BPPD has determined that MON 89034 and MON 89034 x MON 

88017 are in the public interest based on criteria (ii) and (iii) mentioned above. Specifically, 

under criteria (ii), both MON 89034 and MON 89034 x MON 88017 should allow growers the 

opportunity to reduce the use of higher risk, and often less effective and more expensive, 

conventional pesticides. A reduction in use of conventional pesticides equates to less potential 
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for adverse effects to human health and the environment. Additionally, MON 89034 and MON 

89034 x MON 88017 provide a wider spectrum of protection against primary and secondary corn 

pests, which should facilitate greater grain quality, a reduction of mycotoxin contamination, 

increased yield and ultimately have positive implications for human health. 

1. Agricultural Benefits 

MON 89034 

BPPD recognizes that MON 89034’s unique combination of Cry1A.105 and Cry2Ab2 proteins 

expands the spectrum of protection for corn against lepidopteran pests - past that offered by 

already-registered MON 810 (BPPD, 2007a). In addition to providing protection against primary 

pests such as European corn borer (Ostrinia nubilalis, ECB), MON 89034 also protects against 

secondary corn pests such as corn earworm (Helicoverpa zea, CEW), fall armyworm 

(Spodoptera frugiperda, FAW), and black cutworm (Agrotis ipsilon, BCW) (BPPD, 2007a; 

BPPD, 2007c; BPPD, 2007d). Use of MON 89034 could reduce or eliminate the need for 

conventional pesticide applications on acreage infested with secondary pests, although most 

growers do not use conventional pesticides to treat pests that are not part of the soil pest complex 

(BPPD, 2001). Finally, yield appears to be comparable to other Bt insect-protected corn. In 

situations of increased lepidopteran pressure, yield could be higher than other Bt insect-protected 

corn because of the presence of two insecticidal toxins and the effective protection against 

particular primary and secondary corn pests. 

MON 89034 x MON 88017 

In addition to the agricultural benefits mentioned above for MON 89034, MON 89034 x MON 

88017 provides control of corn rootworm complex (Diabrotica spp., CRW) that is functionally 

equivalent to already-registered MON 863 and MON 88017. Use of MON 89034 x MON 88017 

should encourage replacement and reduction of higher-risk conventional pesticides currently 

utilized for CRW control (BPPD, 2003). Additionally, MON 89034 x MON 88017, which has 

tolerance for glyphosate, should allow corn growers to utilize a conventional chemical, Roundup, 

that is recognized by the Agency as a Category E chemical (i.e., there is evidence of noncarcinogenicity for humans). Finally, yield appears to be comparable to other Bt insect-protected 

corn. In situations of increased lepidopteran and/or coleopteran pressure, yield could be higher 

than other Bt insect-protected corn because of the presence of three insecticidal toxins and the 

effective protection against particular primary and secondary corn pests. 
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2. Economic (Grower) Benefits 

MON 89034 

MON 89034 will offer protection against a wider spectrum of primary and secondary corn pests 

(including FAW and CEW); should create conditions that allow for a reduction in the amount of 

mycotoxin contamination; and should facilitate replacement and reduction of the amount of a 

small amount of conventional pesticides that may be used against particular non-soil complex 

corn pests. It is reasonable to believe that all of these characteristics should result in increased 

yield, increased grain quantity, and increased grain quality. 

 

MON 89034 x MON 88017 

Because of the presence of MON 88017, which offers protection against CRW, MON 89034 x 

MON 88017 should offer the same benefits as MON 89034 with perhaps more reduction in 

conventional pesticide use and a slight advantage over the single event for growers that require 

protection against lepidopteran pests and CRW. 

3. Human Health and Environmental Benefits 

MON 89034 

Human Health 

The Cry1A.105 and Cry2Ab2 proteins produced by MON 89034 should not present toxicity or 

allergenicity problems in humans based on the reviews of the studies submitted in support of 

MON 89034’s conditional registration. As with other Bt corn products, it is reasonable to 

assume that the utilization of MON 89034 should reduce the use of some conventional pesticides 

(BPPD, 2001). Finally, because the Cry1A.105 and Cry2Ab2 proteins target secondary corn 

pests - such as FAW and CEW - and protect the ear from damage caused by these pests, 

decreased amounts of mycotoxin contamination should be recognized as a substantial benefit. 

Environmental 

Generally, there should be no risk from the proposed uses for MON 89034 to non-target 

organisms, including, mammalian wildlife species, aquatic species, avian species, non-target 

insects, and endangered species (BPPD, 2007i; BPPD, 2007j). A recently published laboratory 

study shows reduced growth in shedding caddis flies exposed to anti-lepidopteran Cry1A protein 

corn litter (Rosi-Marshall, et al. 2007), therefore, BPPD will require additional aquatic 

invertebrate data. A 21-day Daphnia study, per the 885 Series Office of Prevention, Pesticides, 

and Toxic Substances (OPPTS) Guidelines, will be submitted as a condition of registration. 

Finally, use of MON 89034 should encourage a small reduction in the use of conventional 
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pesticides. Fewer chemical insecticide applications generally result in increased populations of 

beneficial organisms that control secondary pests, such as aphids and leafhoppers. 

MON 89034 x MON 88017 

Human Health 

In addition to Cry1A.105 and Cry2Ab2 proteins produced in MON 89034, the introduction of 

MON 88017 in the pyramided product results in production of Cry3Bb1 protein. Human risk 

assessment data has previously been reviewed for MON 88017, and BPPD concluded that there 

is reasonable certainty that no harm will result from aggregate exposure to the U.S. population, 

including infants and children (BPPD, 2007g). Additionally, an exemption from tolerance was 

established for Cry3Bb1 protein under 40 CFR 174.518. As with other Bt corn products, it is 

reasonable to assume that the utilization of MON 89034 x MON 88017 should reduce the use of 

conventional pesticides. In particular, the use of MON 89034 x MON 88017 should result in the 

reduction of many conventional pesticides that are currently used, which have significant adverse 

effects on human health (BPPD, 2003). 

Environment 

Cry3Bb1 protein, produced in MON 88017, posed no significant risk to test organisms (BPPD, 

2003). The only potential concern, brought to BPPD’s attention by a recently published study, 

relates to MON 89034 and will be dealt with by submission of 21-day Daphnia study. 

Additionally, use of MON 89034 x MON 88017 should reduce the amount of conventional 

pesticides used in the environment. All of the conventional pesticides used for CRW control or 

suppression currently cause significant adverse environmental effects under conditions of normal 

use (BPPD, 2003). Fewer chemical insecticide applications generally result in increased 

populations of beneficial organisms that control secondary pests, such as aphids and leafhoppers. 

4. Insect Resistance Management (IRM) Benefits 

MON 89034 (mention of MON 89034 assumes the same conclusions for MON 89034 x MON 

88017 also) 

BPPD concludes that MON 89034’s two modes of action are better than a single mode of action 

for mitigating the development of insect resistance. But, because of uncertainties in the data 

submitted to support the MON 89034 IRM plan, a 5% refuge cannot be established until 

additional data is submitted to support such a reduction. Instead, BPPD recommends that the 

separate refuge option include a 20% lepidopteran refuge (as has been required for other Bt

products). A 20% refuge is likely to be supported for MON 89034 in cotton-growing regions of 

the southeastern U.S. where a 50% refuge has been previously required. This will likely provide 

an economic benefit to certain growers, since they will be required to plant less structured 

refuge. In addition, the Cry1A.105 and Cry2Ab2 toxins are new proteins targeting lepidopteran 
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pests in corn. These additional modes of action will likely provide a benefit to IRM programs 

(i.e., a toxin “mosaic” in corn-growing regions may reduce the likelihood of resistance 

developing in individual toxins). Also, the use of pyramided Bt corn products (containing 2 or 

more toxins targeting the same pest) should further reduce the potential for resistance (BPPD, 

2007b; BPPD, 2007h). 

BACKGROUND

1. General Information 

Corn (Zea mays L.) is the largest cultivated crop grown in the United States in terms of acreage 

planted and net value. Monsanto states that 93.6 million of acres of corn were planted in the 

U.S. during 2007 and that the net value of the 2006 corn crop was 33.7 billion dollars. The corn 

industry suffers substantial economic losses from damage caused by specific lepidopteran and 

coleopteran pests. 

Two primary corn pests of particular concern to growers are corn rootworm complex (Diabrotica 

spp., CRW) and European corn borer (Ostrinia nubilalis, ECB). According to Monsanto, CRW 

causes damage to all portions of the plant (i.e., those above and below ground) depending on the 

insect’s life stage. In 2003, EPA estimated that approximately 28 million acres of corn were 

infested with CRW and that untreated corn could result in severe yield loss, which was typically 

in the range of 8 -16% reduction, but could be as high as 28% (BPPD, 2003). ECB has been 

identified as the second most important insect pest of corn after CRW. ECB causes damage to 

the plant based upon the generation: (i) the first generation causes leaf and stalk damage; (ii) the 

second generation causes stalk, leaf sheath, collar, and ear damage; and (iii) the third generation 

causes leaf sheath, collar, and ear damage. Monsanto estimates that the average annual U.S. 

yield loss from ECB infestation is within the range of 3-7%. Deviations from the range are 

attributed to level of infestation and region. 

Two secondary corn pests of particular concern to growers are corn earworm (Helicoverpa zea, 

CEW) and fall armyworm (Spodoptera frugiperda, FAW). FAW typically has a limited range as 

it is primarily found in the Gulf States and overwinters only in extreme southern Texas and 

Florida. Monsanto provides an estimate that FAW damage to untreated acreage in Georgia 

between 1991 and 1997 resulted in average yield loss of approximately 10%. No average yield 

loss for all of the U.S. due to FAW damage was provided. On the other hand, CEW is found 

throughout the U.S. corn-growing region, but Monsanto cites its economic damage as being low 

and dependent on timing of infestation, region, and number of moth flights per year. 

2. MON 89034 

Monsanto has developed MON 89034, a corn product that produces Bacillus thuringiensis (Bt)-

derived insecticidal proteins Cry1A.105 and Cry2Ab2. The Cry1A.105 toxin is a “chimeric” 

protein containing domains I and II and the C-terminal from Cry1Ac and domain III from Cry1F. 
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The Cry2Ab2 protein is functionally equivalent to that currently expressed in Monsanto’s 

Bollgard II cotton. MON 89034 is protected from damage caused by larval feeding of ECB, 

southwestern corn borer (Diatraea grandiosella, SCWB), Sugarcane borer (Diatraea 

saccharalis, SCB), FAW, and CEW (BPPD, 2007a). 

3. MON 89034 x MON 88017 

Monsanto has also developed a second generation corn product, MON 89034 x MON 88017. 

MON 88017 (EPA Reg. No. 524-551) (plasmid vector ZMIR39) expresses the Cry3Bb1 Bt toxin 

and is targeted against CRW larvae. The toxin is the same as expressed by MON 863 corn (EPA 

Reg. No. 525-528), which was registered by Monsanto for the 2003 growing season. The 

Cry3Bb1 protein produced in MON 88017 and MON 863 is a variant of the wild-type Cry3Bb1 

protein from Bt subsp. kumamotoensis. When compared by amino acid sequencing, the Cry3Bb1 

protein expressed in MON 88017 has been reported to be 99.8% similar to the Cry3Bb1 protein 

expressed in MON 863. The primary difference between the two hybrids is that MON 88017 

also expresses a gene for resistance to glyphosate (Roundup)-based herbicides (BPPD, 2005). 

By crossing MON 89034 and MON 88017 through conventional breeding, Monsanto has 

obtained an insect-protected corn product that expresses the Cry1A.105, Cry2Ab2, and Cry3Bb1 

Bt toxins, is targeted against lepidopteran corn pests including ECB, SWCB, CEW, and FAW as 

well as coleopteran CRW, and is tolerant of glyphosate (BPPD, 2007b). 

4. Monsanto’s Public Interest Assertions for MON 89034 and MON 89034 x MON 88017 

In the introduction of their public interest document (PID), Monsanto outlines the following 

reasons why MON 89034 and MON 89034 x MON 88017 are in the public interest according to 

some of the criteria set forth in 51 Fed. Reg. 7628: 

 Enhanced spectrum of control. MON 89034 provides protection against 

an expanded spectrum of lepidopteran pests when compared to current Bt corn products. 

MON 89034 x MON 88017 protects against both particular lepidopteran and coleopteran 

pests. The increased protection found in both products improves overall grain quality and 

limits yield losses due to root, leaf, stalk, and ear damage. 

 Reduced mycotoxin levels. Because MON 89034 and MON 89034 x MON 88017 

control the secondary pests, FAW and CEW, the opportunity for fungal infections 

to thrive due to plant damage is reduced. This leads to less mycotoxin contamination. 

 Improved breeding efficiency. Vector-stacking, which increases the efficiency of 

breeding multiple traits into new corn hybrids, was utilized in the creation of 

MON 89034 and MON 89034 x MON 88017. 

 Compatibility with integrated pest management (IPM) systems. Both MON 89034 
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and MON 89034 x MON 88017 provide two different modes of action in a single plant 

and reduce the probability of lepidopteran pests developing resistance to the Bt proteins. 

This allows for a smaller refuge, helps the product maintain efficacy, and guards against 

potential insect resistance to Bt crops. 

 Reduced use of chemical pesticides. MON 89034 and MON 89034 x MON 88017 

reduce the use of conventional chemicals, which saves costs and protects human 

health and the environment. 

 Easy implementation. No additional labor or machinery is needed to plant, grow, 

or harvest MON 89034 and MON 89034 x MON 88017 relative to conventional 

corn. 

 Presence of glyphosate tolerance. MON 89034 x MON 88017 produces 

5-enolpyruvylshikimate-3-phosphate synthase protein from Agrobacterium sp. 

Strain CP4, which confers tolerance to glyphosate. Therefore, the agricultural 

herbicide, Roundup, can be utilized on MON 89034 x MON 88017 corn to 

control weeds and enhance the ability of the corn plants to access soil nutrients. EPA has 

classified glyphosate as a Category E Chemical, meaning there is evidence of noncarcinogenicity for humans. 

This review will evaluate most of the assertions presented above in order to determine if MON 

89034 and MON 89034 x MON 88017 are in the public interest. 

 

EPA’S REVIEW OF MONSANTO’S PUBLIC INTEREST DOCUMENT

Monsanto submitted a public interest document in support of the Section 3(c)(7)(C) registrations 

of MON 89034 and MON 89034 x MON 88017 (Crawford and Bogdanova 2007, MRID 

472797-01). The main portion of this document is divided into the following five sections: (i) 

agricultural benefits; (ii) economic (grower) benefits; (iii) human health benefits; (iv) 

environmental benefits; and (v) insect resistance management benefits. This document also 

includes three appendices: Appendix I provides a summary of reports submitted by Monsanto to 

the EPA that support registration of MON 89034 and MON 89034 x MON 88017, Appendix II 

contains a study that analyzes the mycotoxin levels in grain of MON 89034 corn exposed to 

lepidopteran insect infestation and inoculation with Aspergillus flavus or Fusarium 

verticillioides, and Appendix III contains an assessment of efficacy of MON 89034 x MON 

88017 corn against corn rootworm complex (Diabrotica spp., CRW) in the US during 2005 and 

2006. Information provided by Monsanto will be discussed below, as applicable. 
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1. Agricultural Benefits

a) Pest Spectrum and Efficacy - Monsanto’s Summary (MRID 472797-01) 

MON 89034 

MON 89034 exhibits the Cry proteins, Cry1A.105 and Cry2Ab2, which specifically target 

lepidopteran pests (See Tables 7 and 8). The primary benefit of MON 89034 is that it provides 

equal (as compared to MON 810) or improved protection (as compared to MON 810, other Bt

corn products, and non-Bt corn products) from feeding damage caused by particular lepidopteran 

pest larvae. The spectrum of protection against lepidopteran insects includes the following: 

European corn borer (Ostrinia nubilalis, ECB), southwestern corn borer (Diatraea grandiosella,

SCWB), Sugarcane borer (Diatraea saccharalis, SCB), fall armyworm (Spodoptera frugiperda, 

FAW), black cutworm (Agrotis ipsilon, BCW), and corn earworm (Helicoverpa zea, CEW). 

During the 2003 and 2004 growing seasons, Monsanto conducted efficacy field trials in the U.S., 

Puerto Rico, and Argentina. MON 89034’s control of ECB, SWCB, and SCB was found to be 

comparable to MON 810 (See Table 11). Because of the production of Cry1A.105 protein by 

MON 89034 and the subsequent control of FAW throughout the season and not just the plant’s 

vegetative growth phase, Monsanto claims a higher level of protection and increased activity 

against FAW are shown by MON 89034 as opposed to MON 810 (See Table 11). Of particular 

note, under heavy FAW pressure, MON 810 did not provide the significant amount of protection 

from leaf damage that MON 89034 exhibited. Finally, the Cry2Ab2 protein produced by MON 

89034 provided improved control from CEW, when compared to the activity of MON 810’s 

Cry1Ab protein (See Table 11). 
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Table 9. Summary of arthropod LC50 values for the Cry1A.105 protein exposure in diet 

bioassays 

In the event that no adverse effect was observed, the LC50 value is considered to be greater than the 

maximum concentration tested. 

*Table from page 15 of MRID 472797-01 

1

 LC50 values with a greater than sign represent the highest dose tested. 2

 Assay was performed with lyophilized leaf tissue from MON 89034. 
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Table 9. (cont). Summary of arthropod LC50 values for the Cry1A.105 protein exposure in 

diet bioassays 

In the event that no adverse effect was observed, the LC50 value is considered to be greater than the 

maximum concentration tested. 

*Table from page 16 of MRID 472797-01 

1

 LC50 values with a greater than sign represent the highest dose tested. 2 The no observed effect concentration was determined to be 120 μg/g diet. 
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Table 10. Summary of arthropod LC50 values for the Cry2Ab2 protein exposure in diet 

bioassays 

In the event that no adverse effect was observed, the LC50 value is considered to be greater than the 

maximum concentration tested. 

*Table from page 17 of MRID 472797-01 

1

 LC50 values with a greater than sign represent the highest dose tested. 

2

 42% mortality was observed at the lowest tested dose of 100 g/mL diet. 

3

 61% mortality was observed at the lowest tested dose of 50 g/mL diet. 

4

 Significant mortality was not observed at the highest tested dose of 100 ug/mL diet, 

however, at the highest tested dose of 100 ug/mL diet >95% growth inhibition was observed 

relative to the control treatment in three independent assays 

5

 Assay was performed with lyophilized leaf tissue derived from MON 89034. 
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Table 10. (cont). Summary of Arthropod LC50 Values for the Cry2Ab2 Protein Exposure 

in Diet Bioassays 

In the event that no adverse effect was observed, the LC50 value is considered to be greater than the 

maximum concentration tested. 

*Table from page 18 of MRID 472797-01 

1

 LC50 values with a greater than sign represent the highest dose tested. 

 
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 Table 11. Summary of field efficacy of MON 89034, MON 810 and control corn against 

major lepidopteran pests during the 2003-2004 growing season 

FField 

Location

Infestation method Damage 

measured

fInfestation 

level

Trait 

performance1

Fall Armyworm

Puerto Rico 

(I)

Natural Leaf High MON 

89034>MON 

810>Control

Puerto Rico 

(II)

Natural Leaf Severe MON 

89034>MON 

810=Control

U.S. Artificial Leaf 50 larvae /plant MON 

89034>MON 

810>Control

Argentina Natural Leaf Low MON 

89034>MON 

810>Control

Corn Earworm

Puerto Rico 

(I)

Natural Ear Moderate MON 

89034>MON 

810>Control

U.S. Artificial Ear 15 larvae /plant MON 

89034>MON 

810>Control

Argentina Natural Ear Low-moderate MON 

89034>MON 

810>Control

Southwest Corn Borer

U.S. Artificial Stalk tunneling 7 larvae /plant MON 

89034=MON 

810>Control

European Corn Borer

U.S. Artificial Stalk tunneling 50 larvae /plant MON 

89034=MON 

810>Control

Sugarcane Borer

Argentina Natural Stalk tunneling Moderate-high MON 

89034=MON 

810>Control

*Table from page 20 of MRID 472797-01 

1

 Level of protection against lepidopteran pest damage. 

 > represents statistically significantly improved performance compared to other treatment 

 = represents no statistically significant difference in performance
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MON 89034 x MON 88017 

In addition to producing the Cry1A.105 and Cry2Ab2 proteins, MON 89034 x MON 88017 also 

produces the insecticidal protein, Cry3Bb1, that controls damage caused by CRW. 

The efficacy of MON 89034 x MON 88017 against CRW was compared in field trials in the 

U.S. in 2005 and 2006, and against lepidopteran pests in 2006. The pyramided product showed 

protection from feeding damage by lepidopteran pests that was comparable to MON 89034, as 

well as protection from damage by CRW that was comparable to MON 88017. The average root 

damage rating (RDR) for MON 88017 and MON 89034 x MON 88017 was significantly less 

than the RDR for non-CRW protected controls (See Table 12). 

Table 12. Field efficacy of MON 88017 and MON 89034 x MON 88017 and non-CRWprotected control corn against corn rootworm tested in 2005 and 2006 

Entry RDR1,2 

2005 

Control 1.399 A 

MON 88017 0.165 B 

MON 89034 x MON 88017 0.164 B 

2006 

Control 0.774 A 

MON 89034 x MON 88017 0.092 B 

 *Table from page 21 of MRID 472797-01 

1 RDR - Root damage rating calculated as a least-square mean of n=5 plants per plot in 2005 and n = 6 

plants per plot in 2006. 

2 Values indicated by the same letter in the same column are not statistically different (Fisher's protected 

LSD p=0.05 level). 

The efficacy of MON 89034 x MON 88017 against ECB was also assessed in 2005 U.S. trials. 

Significantly less feeding was observed on MON 89034 and MON 89034 x MON 88017 and 

these two insect-protected corn crops also provided a high level of control against leaf damage 

and stalk tunneling by ECB. 
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a) Pest Spectrum and Efficacy – BPPD’s Response 

MON 89034 

BPPD agrees with Monsanto that MON 89034 targets lepidopteran pests specifically. In two 

studies conducted by Monsanto, both the Cry1A.105 protein and Cry2Ab2 protein exhibited 

insecticidal activity in the order Lepidoptera but not in the orders Coleoptera and Hemiptera 

(BPPD, 2007c; BPPD, 2007d). The Cry1A.105 protein, administered at 50 μg/mL and 100 

μg/mL concentrations to insects in the orders Lepidoptera, Coleoptera, and Hemiptera, caused a 

range of mortality of 32% to 96% in CEW, ECB, and FAW (BPPD, 2007c). Additionally, all 

four lepidopteran insects (CEW, ECB, FAW, and BCW) had a range of 32% to 100% growth 

stunting (BPPD, 2007c). On the other hand, the Cry2Ab2 protein, administered at 50 μg/mL and 

100 μg/mL concentrations to insects in the orders Lepidoptera, Coleoptera, and Hemiptera, 

caused at least 61% mortality (corrected) against CEW, ECB, and FAW (BPPD, 2007d). All 

four lepidopteran insects (CEW, ECB, FAW, and BCW) had a range of 97 to 100% growth 

stunting (BPPD, 2007d). 

BPPD agrees with Monsanto’s conclusions from the field trials conducted in the U.S., Puerto 

Rico, and Argentina. Across all geographies, the efficacy of MON 89034 against ECB, SWCB, 

CEW, FAW, and SCB was equal to or greater than that of YieldGard Corn Borer (MON 810), a 

lepidopteran control corn product that expresses the Cry1Ab protein. However, MON 89034 did 

offer a broader spectrum of insect protection activity than MON 810 and demonstrated better 

control of CEW, FAW, and SCB than MON 810 in these trials (BPPD, 2007a). 

MON 89034 x MON 88017 

In the past, BPPD has concluded that MON 88017 is functionally equivalent to MON 863 for 

CRW control (BPPD, 2005). Therefore, BPPD finds the efficacy benefits of MON 89034 x 

MON 88017 are similar to the efficacy benefits of MON 863 (BPPD, 2003) and MON 88017 

(BPPD, 2005). A summary of these benefits can also be found in BPPD’s Biopesticides 

Registration Action Document – Bacillus thuringiensis Plant-incorporated Protectants (BPPD, 

2001). BPPD agrees that the efficacy of MON 89034 x MON 88017 should be comparable to 

efficacy of the MON 89034 and MON 88017 isolines for FAW and western corn rootworm 

(WCRW, Diabrotica virgifera) and to the MON 89034 isoline for ECB, SWCB, CEW, and 

FAW. Furthermore, similar to MON 89034, MON 89034 x MON 88017 provides a broader 

spectrum of efficacy than MON 810 against lepidopteran pests. Although no SCB, WBCW, or 

BCW field trials were conducted with MON 89034 x MON 88017, based on the efficacy against 

the other pests, it is reasonable to assume comparable efficacy to MON 89034 for these pests as 

well (BPPD, 2007b). 
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b) Yield – Monsanto’s Summary (MRID 472797-01) 

MON 89034 and MON 89034 x MON 88017 

In 2006, Monsanto conducted field trials with the objective of comparing yield between MON 

89034, MON 89034 x MON 88017, MON 810, MON 810 x MON 88017, and other hybrids not 

producing Bt proteins. Results showed comparable yield across several hybrids tested for insectprotected hybrids and higher yields compared to hybrids that did not produce Bt proteins (See 

Table 13). Additionally, the assumption is made that under intense lepidopteran pressure, the 

yield benefit from MON 89034 would be significantly higher. Although no economic benefits 

can be assessed for MON 89034 until it is actually used by growers, Monsanto predicts that the 

economic benefits would be equal or even more advantageous depending on the level and type of 

pest infestation that occurs. 

Table 13. Yield comparison between MON 89034, MON 89034 x MON 88017, MON 810, 

MON 810 x MON 88017, and non-Bt hybrids grown in the U.S. during 2006 

Product Yield (Bu/Acre) Number of hybrids 

tested 

MON 89034 180.4 60 

MON 810 180.6 29 

MON 89034 x MON 88017 189.3 80 

MON 810 x MON 88017 185.7 36 

Non-Bt 171.5 2 

 *Table from page 13 of MRID 472797-01 

c) Yield – BPPD’s Response 

BPPD believes that it is reasonable for Monsanto to assume that significant pressure from 

lepidopteran pests would cause the yield benefit for MON 89034 to be higher because of the 

presence of 2 insecticidal toxins, Cry1A.105 and Cry2Ab2. Furthermore, if the pressure is from 

secondary corn pests such as FAW and CEW, then increase of yield is even more logical. 

Although Monsanto assumes that economic benefits will be equal or more advantageous for 

MON 89034 and is probably correct, BPPD will not assume complete validity of this assumption 

until MON 89034 is used over the course of several years and reliable yield data is available. 

Although use of MON 89034 x MON 88017 could also result in an overall increase in yield, 

BPPD does not expect an increase in yield that exceeds that of the previously-registered single 

gene MON 88017 as any increase in yield will result mostly from the characteristics of MON 

89034: expanded pest spectrum and the presence of two toxins instead of one. 
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2. Economic (Grower) Benefits 

a) Monsanto’s Summary (MRID 472797-01) 

MON 89034 

For economic benefits, Monsanto cites to National Center for Food and Agricultural Policy 

statistics that are based on planted acreage of MON 810 corn in 2005. Monsanto estimated that 

MON 810 increased corn production by 103.9 million bushels because of the corn borer resistant 

trait. Net returns, decreased costs (fuel, labor, and conventional pesticides purchased), and 

premium price of protected seeds were estimated to be 197 million dollars. The decreased use of 

conventional pesticides was estimated at 4.85 million pounds. In percentage terms, MON 810 

planted in 2005 resulted in a 24% increase in yield, a 27% decrease in pesticide use, and 

increased monetary gain of 26% when compared to 2004. 

The major economic benefits of MON 89034 are the following: (i) a wider spectrum of pest 

protection (to include FAW and CEW), which results in increased grain quality and increased 

yield; (ii) reduction in mycotoxin contamination levels which contributes to economic recovery; 

(iii) protection that is more effective in controlling corn borers and therefore results in increased 

yield, grain quality, and grain quantity; and (iv) reduction in conventional pesticide use that 

results in less costs (See Table 14). 

Table 14. Summary of economic benefits to growers using corn-borer protected corn 

Benefit Per acre benefit ($) Total benefit 

($ Millions) 

Yield increase 13.59 (-3.67 – 48.76) 217 (-59 – 780) 

Pesticide reduction 1.99 (1.00 – 2.98) 32 (16 – 48) 

Mycotoxin reduction 1.98 (0.52 – 7.12) 32 (8.3 – 114) 

 *Table from page 29 of MRID 472797-01 

MON 89034 x MON 88017 

Monsanto states that the pyramided product will offer the combined benefits of the individual 

parents, MON 89034 and MON 88017. The addition of MON 88017 creates enhanced 

protection against CRW, a primary corn pest that can cause total yield losses that exceed $1 

billion dollars annually, and adds the glyphosate tolerance trait that limits yield loss from weed 

pressure. Overall, MON 89034 x MON 88017 will limit yield losses from corn borer insects, 

CRW, and weed pressure, reduce conventional pesticide use, and reduce mycotoxin 

contamination while increasing yield, grain quantity, and grain quality. 

 
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b) BPPD’s Response 

MON 89034 

Overall, BPPD agrees that MON 89034 should produce economic benefits for many growers. 

Monsanto’s numbers are estimates and actual economic benefits may be affected by factors 

including pest pressure, climatic fluctuations, and commodity pricing. MON 89034 is effective 

against a wider spectrum of corn pests and it is reasonable to assume that this should result in 

increased yield. Additionally, BPPD has concluded that a slight decrease in pesticide use should 

be realized with the use of Bt corn products similar to MON 89034 (BPPD, 2001); therefore, 

BPPD agrees with Monsanto’s assertion of possible conventional pesticide use reduction and 

associated reduced costs. 

BPPD agrees with Monsanto’s conclusion that MON 89034 should reduce mycotoxin 

contamination. Overall, if primary and secondary corn pest pressure is reduced, then less 

mycotoxin contamination will be present, which will in turn lead to increased yield, grain 

quantity, and grain quality (BPPD, 2001). Furthermore, field evidence has demonstrated the 

ability of Bt corn to reduce the infestation rates of certain mycotoxins (Wu, 2008). This article 

specifically associates CEW with aflatoxin accumulation in corn and claims that Bt corn 

varieties, perhaps those such as MON 89034, are being developed to combat this insect pest in 

order to reduce particular mycotoxin contamination. 

MON 89034 x MON 88017 

BPPD believes that use of MON 89034 x MON 88017, much like MON 89034, should produce 

the same economic benefits mentioned above. Additionally, the combination with MON 88017, 

which protects corn against CRW and exhibits glyphosate tolerance, can be expected to create 

slightly greater economic benefits than MON 89034 for growers needing to treat both 

lepidopteran pests and CRW. The economic benefits of MON 88017 (minus an evaluation of 

glyphosate tolerance) have previously been assessed in MON 863’s public interest finding 

document (BPPD, 2003). 

3. Human Health and Environmental Benefits 

a) Monsanto’s Summary (MRID 472797-01) 

MON 89034 

Human Health 

Monsanto states that the Cry1A.105 and Cry2Ab2 proteins produced by MON 89034 are 

structurally and functionally related to Cry proteins that have a history of use both as active 

ingredients in Bt microbial pesticides and bio-tech derived food and feed. Furthermore, they 
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state that Bt has been commercially used in the U.S. since 1958 to produce microbial-derived 

pesticides and no adverse effects on humans or animals has been reported during their use on 

food or feed crops. Additionally, Monsanto asserts that Cry1A.105 and Cry2Ab2 proteins are 

highly unlikely to create any concerns of toxicity or allergenicity to humans. These assertions 

are based on acute oral toxicity data, which produced results that agreed with literature showing 

that Bt proteins only impact insect species and that no mammalian toxicity or issues have been 

reported in nearly 60 years of Bt protein insecticide use, and a comparison to known allergens, 

which indicated no allergenicity in the Cry1A.105 and Cry2Ab2 proteins. 

Two of the specific human health benefits that Monsanto attributes to use of MON 89034 are 

pesticide reduction and mycotoxin reduction. Using numbers from an article from the National 

Center for Food and Agricultural Policy, Monsanto demonstrates current usage levels of MON 

810, a functional equivalent of MON 89034, results in an estimated decrease in use of 

approximately 4.85 million pounds of conventional pesticides per year (equivalent to a 27% 

decrease in conventional pesticide use to control corn-boring pests). According to Monsanto, 

mycotoxin reduction is also evident with the use of MON 89034 because of its ability to suppress 

or control secondary corn pests, such as CEW and FAW, that play a role in damaging corn ears 

and facilitating the inoculation and growth of mycotoxin-producing fungi. Monsanto cites to two 

types of fungi, Fusarium and Aspergillus that produce fumonisin and aflatoxin, respectively. A 

study conducted by Monsanto indicates that MON 89034 is subject to less damage from corn 

pests and subsequently, it suffers less mycotoxin contamination, particularly from Aspergillus. 

Environmental

Monsanto states that Cry1A.105 and Cry2Ab2 have no toxic effects on non-target organisms (to 

include the following: mammalian wildlife species, aquatic species, avian species, non-target 

insects, and endangered species) based on studies they submitted to the Agency in conjunction 

with the registration application for MON 89034. Additionally, the proteins rapidly degrade in 

soil which also minimizes exposure to non-target species. 

MON 89034 x MON 88017 

Human Health 

Since MON 89034 is present in the pyramided product, Monsanto’s contentions for the safety of 

MON 89034 with regard to human health also apply to MON 89034 x MON 88017. In addition 

to the presence of the Cry1A.105 and Cry2Ab2 proteins found in MON 89034, the pyramided 

product also produces Cry3Bb1 protein. Given that MON 88017 is already a product registered 

by the EPA, Monsanto states that the Cry3Bb1 protein produced by MON 89034 x MON 88017 

already has an exemption from tolerance. Furthermore, human risk assessment data reviewed by 

the EPA for registration of MON 88017 has resulted in a conclusion that there is reasonable 

certainty that no harm will result from aggregate exposure to the U.S. population, including 

infants and children. As with MON 89034, MON 89034 x MON 88017 is expected to result in 
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both reduced pesticide use (and perhaps more because of MON 88017’s protection from primary 

corn pest, CRW) and mycotoxin contamination. 

Environmental 

Since MON 89034 is present in the pyramided product, Monsanto’s contentions for the safety of 

MON 89034 with regard to environmental effects also apply to MON 89034 x MON 88017. 

MON 89034 x MON 88017 also produces the Cry3Bb1 protein, which is in the previously 

registered MON 88017. Upon review of the environmental effects data for MON 88017’s 

registration, EPA concluded that no unreasonable adverse effects are expected to the 

environment from the cultivation of MON 88017 and MON 88017 x MON 810 corn. 

b) BPPD’s Response 

MON 89034 

Human Health 

BPPD agrees with Monsanto’s conclusions that the Cry1A.105 and Cry2Ab2 proteins produced 

by MON 89034 should not cause toxicity or allergenicity problems in humans. The data 

submitted and cited regarding potential health effects for the Cry1A.105 and Cry2Ab2 proteins 

include the characterization of the expressed proteins in corn, as well as acute oral toxicity 

studies, amino acid sequence comparisons to known allergens and toxins, and in vitro 

digestibility of the proteins. The results of these studies were used to evaluate human risk 

(BPPD, 2007e). 

 

The acute oral toxicity data submitted support the prediction that the Cry1A.105 and Cry2Ab2 

proteins would be non-toxic to humans. When proteins are toxic, they are known to act via acute 

mechanisms and at very low dose levels. Since no treatment-related adverse effects were shown 

to be caused by the Cry1A.105 and Cry2Ab2 proteins, even at relatively high dose levels, the 

Cry1A.105 and Cry2Ab2 proteins are not considered toxic. Basing this conclusion on acute oral 

toxicity data without requiring further toxicity testing or residue data is similar to the Agency 

position regarding toxicity and the requirement of residue data for the microbial Bt products from 

which this plant-incorporated protectant was derived (See 40 CFR 158.740(b)(2)(i)) (BPPD, 

2007e). 

Since Cry1A.105 and Cry2Ab2 are proteins, potential allergenicity was also considered as part 

of the toxicity assessment. Considering all of the available information (1) Cry1A.105 and 

Cry2Ab2 originate from a non-allergenic sources; (2) Cry1A.105 and Cry2Ab2 have no 

sequence similarities with known allergens; (3) Cry1A.105 and Cry2Ab2 are not glycosylated; 

and (4) Cry1A.105 and Cry2Ab2 are rapidly digested in simulated gastric fluid; EPA has 

concluded that the potential for Cry1A.105 and Cry2Ab2 to be a food allergens is minimal 

(BPPD, 2007e). 
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The lack of mammalian toxicity at high levels of exposure to the Cry1A.105 and Cry2Ab2 

proteins, as well as the minimal potential to be a food allergens, demonstrate the safety of the 

product at levels well above possible maximum exposure levels anticipated (BPPD, 2007e). 

BPPD agrees with Monsanto’s conclusion that MON 89034 should reduce mycotoxin 

contamination. Overall, if primary and secondary corn pest pressure is reduced, then less 

mycotoxin contamination will be present, which will in turn lead to increased yield, grain 

quantity, and grain quality (BPPD, 2001). Further, field evidence has demonstrated the ability of 

Bt corn to reduce the infestation rates of certain mycotoxins (Wu, 2008). This article specifically 

associates CEW with aflatoxin accumulation in corn and claims that Bt corn varieties, perhaps 

such as MON 89034, are being developed to combat this insect pest in order to reduce particular 

mycotoxin contamination. 

Environmental 

BPPD agrees with Monsanto’s assessment that there should be no risk from the proposed uses 

for MON 89034 to non-target organisms, including mammalian wildlife species, aquatic species, 

avian species, non-target insects, and endangered species (BPPD, 2007i; BPPD, 2007j). Based 

on a recently-published laboratory study showing reduced growth in shedding caddis flies 

exposed to anti-lepidopteran Cry1A protein corn litter (Rosi-Marshall, et al. 2007), however, 

BPPD will require additional aquatic invertebrate data. A 21-day Daphnia study, per the 885 

Series Office of Prevention, Pesticides, and Toxic Substances (OPPTS) Guidelines, will be 

submitted as a condition of registration. 

In addition to Monsanto’s submitted rationale for environmental benefits, Monsanto could have 

included a reference to MON 89034 use potentially reducing the amount of conventional 

pesticides applied in the environment and the subsequent environmental benefits. BPPD 

believes that cultivation of MON 89034 corn may have fewer adverse impacts on non-target 

organisms than use of chemical pesticides for corn production, because under normal 

circumstances, MON 89034 corn should require substantially fewer applications of chemical 

pesticides, compared to production of non-Bt corn. Fewer chemical insecticide applications 

generally result in increased populations of beneficial organisms that control secondary pests, 

such as aphids and leafhoppers. 

MON 89034 x MON 88017 

Human Health 

BPPD agrees with Monsanto that the use of MON 89034 x MON 88017 should not result in any 

unreasonable adverse effects to human health. In addition to Cry1A.105 and Cry2Ab2 proteins 

produced in MON 89034, the introduction of MON 88017 results in production of Cry3Bb1 
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protein. BPPD has already reviewed human risk assessment data for MON 88017 and reached a 

conclusion that there is reasonable certainty that no harm will result from aggregate exposure to 

the U.S. population, including infants and children (BPPD, 2007g). Additionally, Cry3Bb1 

currently has an exemption from tolerance established under 40 CFR 174.518. 

BPPD also agrees with the claim that there should be a reduction of some pesticide use (BPPD, 

2001). Additionally, Cry3Bb1 protein primarily protects corn plants against CRW. Virtually all 

of the registered conventional insecticides used to control CRW are of special concern to the 

EPA because of risks to humans. Each year, there are confirmed reports of human illness 

associated with these registered conventional chemicals (See BPPD, 2003). 

Finally, BPPD agrees with the claim that use of MON 89034 x MON 88017 should combat 

mycotoxin contamination because of the production of the Cry1A.105 and Cry2Ab2 proteins (as 

mentioned previously). 

Environmental 

BPPD agrees with Monsanto’s assessment that there should be no risk from the proposed uses 

for MON 89034 x MON 88017 to non-target organisms, including mammalian wildlife species, 

aquatic species, avian species, non-target insects, and endangered species. The only potential 

concern will be addressed through a 21-day Daphnia study. For the registration of MON 88017, 

a series of studies were completed by Monsanto that exposed non-target organisms to high doses 

of leaf tissue, grain, or pollen containing a plant-produced Cry3Bb1 variant or to an artificial diet 

containing a Bt-produced Cry3Bb1 variant. Results indicated that the Cry3Bb1 protein posed no 

significant risk to test organisms (BPPD, 2003). Additionally, a study was conducted on MON 

89034 x MON 88017 to ensure that the interaction between Cry1A.105, Cry2Ab2, and Cry3Bb1 

proteins in the pyramided product would not change the overall properties of each individual 

component. BPPD concluded that the activity of Cry1A.105 and Cry2Ab2 proteins was not 

significantly altered by the presence of Cry3Bb1, and the activity of Cry3Bb1 was not 

significantly altered by the presence of Cry1A.105 and/or Cry2Ab2. The study, along with the 

previously reviewed interaction study between Cry1A.105 and Cry2Ab2, indicated that MON 

89034 x MON 88017 corn should not result in any unexpected interaction with regards to target 

and non-target insects (BPPD, 2007f).

In addition to the submitted rationale for environmental benefits, Monsanto could have included 

reference to MON 89034 x MON 88017 use reducing the amount of conventional pesticides 

applied in the environment and the subsequent environmental benefits. BPPD believes that 

cultivation of MON 89034 x MON 88017 corn may have fewer adverse impacts on non-target 

organisms than use of chemical pesticides for corn production, because under normal 

circumstances, MON 89034 x MON 88017 corn should require substantially fewer applications 

of chemical pesticides, compared to production of non-Bt corn. The reduction in conventional 

pesticide use should essentially be the same seen from MON 88017 and MON 863 use (BPPD, 

2003). Fewer chemical insecticide applications generally result in increased populations of 
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beneficial organisms that control secondary pests, such as aphids and leafhoppers. Furthermore, 

all of the conventional pesticides used for CRW control or suppression cause significant adverse 

environmental effects under conditions of normal use (BPPD, 2003). 

4. Insect Resistance Management (IRM) 

a) Monsanto’s Summary (MRID 472797-01) 

MON 89034 (mention of MON 89034 assumes the same conclusions for MON 89034 x 

MON 88017 also) 

Monsanto establishes that MON 89034, which produces Cry1A.105 and Cry2Ab2 proteins, has 

two different modes of action against lepidopterans, particularly in the way the proteins bind to 

the midgut. Therefore, based on the distinct modes of action of the two proteins and reduced 

likelihood of insect resistance, Monsanto proposes that a reduced structured refuge is possible: 

5% for the corn belt, down from 20% and 20% for cotton-growing regions, down from 50%. 

b) BPPD’s Response 

MON 89034 (mention of MON 89034 assumes the same conclusions for MON 89034 x 

MON 88017 also) 

BPPD agrees with Monsanto in that two modes of action are better than one for reducing the risk 

of insect resistance to MON 89034. But, due to uncertainties in the data submitted to support the 

MON 89034 IRM plan, a 5% refuge cannot be established until additional data is submitted to 

support such a reduction. Instead, BPPD recommends that the separate refuge option include a 

20% lepidopteran refuge (as has been required for other Bt products). A 20% refuge is likely to 

be supported for MON 89034 in cotton-growing regions of the southeastern U.S. where a 50% 

refuge has been previously required (BPPD, 2007b; BPPD, 2007h). 

Overall, MON 89034 should present two immediate IRM benefits: (i) dual (distinct) modes of 

action for Bt corn and (ii) reduced refuge in cotton regions (and the resulting economic benefits 

to growers). These benefits can likely be achieved without an unreasonable risk of resistance to 

Cry1A.105 and Cry2Ab2. Additional grower benefits may be realized in the long term, if a 5% 

refuge can be supported. 

 

5. Efficacy Studies 

In addition to the efficacy studies referenced in the preceeding sections, the following studies 

were submitted and are considered for registration of MON 89034. These studies demonstrate 

the efficacy of MON 89034 corn and the individual Bt proteins (Cry1A.105 and Cry2Ab2) 

against a range of lepidopteran corn pests including European corn borer (ECB), corn earworm 

(CEW), southwestern corn borer (SWCB), fall armyworm (FAW), and sugarcane borer (SCB). 
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MRID 46951413

In laboratory bioassays, the insecticidal activity of Cry1A.105 protein was tested against 

agronomically important insects from the orders Lepidoptera (four species), Coleoptera (two 

species), and Hemiptera (two species). Neonate larvae, nymphs or adults (eggs for western bean 

cutworm prior to hatching) were fed artificial diets containing the appropriate doses of 50 or 100 

μg Cry1A.105/mL of insect diet in the diet-overlay bioassays for fall armyworm, black cutworm, 

European corn borer, corn earworm, Southern corn rootworm, and boll weevil or 40 or 80 μg 

Cry1A.105/mL of insect diet in diet-incorporation bioassays for western tarnished plant bug and 

green peach aphid. Mortality and a reduction in weight or honeydew production over a five or 

seven day period depending on the insect were the endpoints used to indicate insecticidal 

activity. The 50 μg/mL and 100 μg/mL Cry1A.105 concentrations caused a range of 32 to 96% 

mortality in three (corn earworm, European corn borer, and fall armyworm) of the four 

lepidopterans. All four lepidopteran insects had a range of 32-100% stunting. The Cry1A.105 

protein had activity against all four lepidopteran insects, but no activity against the two 

coleopteran or two hemipteran insects tested. Classification: Acceptable

MRID 46951414

In laboratory bioassays, the insecticidal activity of Cry2Ab2 protein was tested against 

agronomically important insects from the orders Lepidoptera (four species), Coleoptera (two 

species), and Hemiptera (two species). Neonate larvae, nymphs or adults (eggs for western bean 

cutworm prior to hatching) were fed artificial diets containing the appropriate doses of 50 or 100 

μg Cry2Ab2/mL of insect diet in the diet-overlay bioassays for fall armyworm, black cutworm, 

European corn borer, corn earworm, Southern corn rootworm, and boll weevil or 40 or 80 μg 

Cry2Ab2/mL of insect diet in diet-incorporation bioassays for western tarnished plant bug and 

green peach aphid. Mortality and a reduction in weight or honeydew production over a five or 

seven day period depending on the insect were the endpoints used to indicate insecticidal 

activity. In the diet-overlay bioassays, both the 50 μg/mL and 100 μg/mL Cry2Ab2 

concentrations caused at least 61% mortality (corrected) against corn earworm, European corn 

borer, and fall armyworm; while stunting was at least 97% for all four lepidopteran insects 

tested. Only the 28% black cutworm mortality resulting from testing against the 50 μg/mL 

Cry2Ab2 concentration failed to meet the study criterion of >30% mortality. The Cry2Ab2 

protein had activity against all four lepidopteran insects, but no activity against the two 

coleopteran or two hemipteran insects tested. Classification: Acceptable

MRID 46951415

Field trials were conducted in 2003-2004 seasons in Puerto Rico, the United States and 

Argentina to determine the efficacy of MON 89034 corn (Cry1A.105 and Cry2Ab2) and MON 

89597 (Cry1A.105 and Cry2Ab2) corn against European corn borer (ECB), corn earworm 

(CEW), southwestern corn borer (SWCB), fall armyworm (FAW), and sugarcane borer (SCB). 

Across all geographies the efficacy of the MON 89034 and MON 89597 against ECB, SWCB, 

CEW, FAW, and SCB was equal to or greater than that of YieldGard® Corn Borer (MON 810), 

a lepidopteran control corn product that expresses the Cry1Ab protein. For all geographies 
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tested, there was no significant difference in efficacy between MON 89034 and MON 89597 

with the exception of CEW damage in PR I, (2003 testing), where MON 89034 demonstrated 

significantly better control than MON 89597. MON 89034 and MON 89597 offer a broader 

spectrum of insect activity than MON 810. MON 89034 and MON 89597 demonstrated 

significantly better control of CEW, FAW, and SCB than MON 810. Details of the field trials 

are found below. Data are acceptable for the Puerto Rico and U.S. trials. Data are 

supplemental for the Argentina trials due to lack of sufficient rationale as to why certain 

locations were excluded from the analysis. No additional data are required. 

III. TERMS AND CONDITIONS OF REGISTRATION 

A. Terms and Conditions for MON 89034 as Amended on December 15, 2008: 

1) The subject registration will automatically expire on midnight September 30, 2010. 

2) The subject registration will be limited to MON 89034 in field or sweet corn. Further, 

MON 89034 sweet corn may only be sold directly to processors or through commercial 

dealers to large growers. MON 89034 sweet corn must not be sold to small roadside or 

home growers. 

 

3) Submit/cite all data required for registration of your product under FIFRA § 3(c)(5) when 

the Agency requires registrants of similar products to submit such data. 

4) This plant-incorporated protectant may be combined through conventional breeding with 

other registered plant-incorporated protectants that are similarly approved for use in 

combination, through conventional breeding, with other registered plant-incorporated 

protectants to produce inbred corn lines and hybrid corn varieties with combined 

pesticidal traits. 

5) Submit the following data in the time frames listed: 

OPPTS Guideline/ 

Study Type 

Required Data Due Date

Residue Analytical 

Method – Plants 

(OPPTS 860.1340) 

For event MON 89034 corn, an independent lab 

validation of the analytical method for the detection of 

Cry2Ab2 and/or Cry1A.105. You must also agree to 

provide to the EPA laboratory (Ft. Meade, MD) 

methodology and/or reagents necessary for validation of 

such analytical method within 6 months from the date 

that the Agency requests them. 

4/1/2009 
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OPPTS Guideline/ Required Data Due Date

Study Type 

Aquatic 

Invertebrate Acute 

Toxicity Testing, 

Freshwater 

Daphnids (OPPTS 

885.4240) 

A 7-14 day Daphnia study as per the 885 Series OPPTS 

Guidelines needs to be performed. Alternatively, a 

dietary study of the effects on an aquatic invertebrate, 

representing the functional group of a leaf shredder in 

headwater streams, can be performed and submitted in 

lieu of the Daphnia study. 

9/1/2009*

Insect Resistance 

Management – 

Resistance 

Monitoring 

Monsanto must provide additional information on crossresistance of Cry1A.105 and Cry1Ac (preferably 

including binding site models and use of resistant 

colonies) for the target pests and determine how such 

cross-resistance may impact the durability of MON 

89034, including any impacts in the southern cottongrowing areas. The Cry1A.105 protein is a chimeric 

protein consisting of Domains I and II and the Cterminus of Cry1Ac. It is important to address not only 

the likelihood of cross-resistance potential of 

Cry1A.105 and Cry1Ab and, similarly, Cry1A.105 and 

Cry2Ab2 (which was done by Monsanto) but also that 

of Cry1A.105 and Cry1Ac. 

7/1/2009*

 
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OPPTS Guideline/ 

Study Type 

Required Data Due Date 

Insect Resistance 

Management – 

Resistance 

Monitoring 

Baseline susceptibility studies and/or a discriminating 

concentration assay are required for the Cry1A.105 

protein against European corn borer (ECB), 

Southwestern corn borer (SWCB), and corn earworm 

(CEW) and for the Cry2Ab2 protein against SWCB and 

CEW. 

7/1/2009*

for ECB 

and CEW 

8/31/2010* 

for SWCB 

Insect Resistance 

Management – 

Resistance 

Monitoring 

To support sweet corn uses, baseline susceptibility 

studies must be conducted on fall armyworm (FAW) 

populations collected from sweet corn growing areas. 

Monitoring studies will be conducted on FAW 

populations collected from sweet corn distribution areas 

in states in which Monsanto MON 89034 and/or MON 

89034 x MON 88017 sweet corn plantings exceed 5,000 

acres. The collected populations of FAW will be 

monitored for changes in susceptibility to the 

Cry1A.105 and Cry2Ab2 proteins. 

4/1/2010 

*

 Extensions were granted as listed in the above table. 

6) The Insect Resistance Management (IRM) terms and conditions for this product are as 

follows. 

The required IRM program for MON 89034 must have the following elements: 

 Requirements relating to creation of a non-Bt corn and/or non-lepidopteran resistant Bt 

corn refuge in conjunction with the planting of any acreage of MON 89034 field corn; 

 Requirements for Monsanto to prepare and require MON 89034 users to sign “grower 

agreements,” which impose binding contractual obligations on the grower to comply with 

the refuge requirements; 

 Requirements regarding programs to educate growers about IRM requirements; 

 Requirements regarding programs to evaluate and promote growers’ compliance with 

IRM requirements; 

 Requirements regarding programs to evaluate whether there are statistically significant 

and biologically relevant changes in target insect susceptibility to Cry1A.105 and 
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Cry2Ab2 proteins in the target insects; 

 Requirements regarding a “remedial action plan,” which contains measures Monsanto 

would take in the event that any field relevant insect resistance was detected as well as to 

report on activity under the plan to EPA; 

 Submit annual reports on units sold by state (units sold by county level will be made 

available to the Agency upon request), IRM grower agreement results, and the 

compliance assurance program including the education program on or before January 31st

each year, beginning in 2010. 

 a) Refuge Requirements for MON 89034 Field Corn 

 These refuge requirements do not apply to seed increase/propagation of inbred and hybrid 

seed corn up to a total of 20,000 acres per county and up to a combined United States (U.S.) total 

of 250,000 acres per plant-incorporated protectant (PIP) active ingredient per registrant per year. 

Furthermore, these refuge requirements do not apply to commercial hybrid sweet corn. 

 1) Corn-Belt Refuge Requirements

 For MON 89034 field corn grown outside cotton-growing areas (e.g., the Corn Belt), grower 

agreements (also known as stewardship agreements) will specify that growers must adhere to the 

refuge requirements as described in the grower guide/product use guide and/or in supplements to 

the grower guide/product use guide. 

 Specifically, growers must plant a structured refuge of at least 5% non-Bt corn and/or 

non-lepidopteran resistant Bt corn that may be treated with insecticides, as detailed 

below, to control lepidopteran stalk-boring and other pests. 

 Refuge planting options include: separate fields, blocks within fields (e.g., along the 

edges or headlands), perimeter strips, and strips across the field. 

 External refuges must be planted within 1⁄2 mile. 

 When planting the refuge as strips across the field or as perimeter strips, refuges must be 

at least 4 consecutive rows wide. 

 Insecticide treatments for control of ECB, CEW, SWCB, and other lepidopteran target 

pests listed on the label, grower guides, or other educational material may be applied only 

if economic thresholds are reached for one or more of these target pests. Economic 

thresholds will be determined using methods recommended by local or regional 

professionals (e.g., Extension Service agents or crop consultants). Instructions to 
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growers will specify that microbial Bt insecticides must not be applied to non-Bt corn 

and/or non-lepidopteran resistant Bt corn refuges. 

 2) Cotton-Growing Area Refuge Requirements

 For MON 89034 field corn grown in cotton-growing areas, grower agreements (also known 

as stewardship agreements) will specify that growers must adhere to the refuge requirements as 

described in the grower guide/product use guide and/or in supplements to the grower guide/ 

product use guide. 

 Specifically, growers in these areas must plant a structured refuge of at least 20% non-Bt

corn and/or non-lepidopteran resistant Bt corn that may be treated with insecticides, as 

detailed below, to control lepidopteran stalk-boring and other pests. 

 Refuge planting options include: separate fields, blocks within fields (e.g., along the 

edges or headlands), perimeter strips, and strips across the field. 

 External refuges must be planted within 1⁄2 mile. 

 When planting the refuge as strips across the field or as perimeter strips, refuges must be 

at least 4 consecutive rows wide. 

 Insecticide treatments for control of ECB, CEW, SWCB, and other lepidopteran target 

pests listed on the label, grower guides, or other educational material may be applied only 

if economic thresholds are reached for one or more of these target pests. Economic 

thresholds will be determined using methods recommended by local or regional 

professionals (e.g., Extension Service agents or crop consultants). Instructions to 

growers will specify that microbial Bt insecticides must not be applied to non-Bt corn 

and/or non-lepidopteran resistant Bt corn refuges. 

 Cotton-growing areas include the following states: Alabama, Arkansas, Georgia, Florida, 

Louisiana, North Carolina, Mississippi, South Carolina, Oklahoma (only the counties of 

Beckham, Caddo, Comanche, Custer, Greer, Harmon, Jackson, Kay, Kiowa, Tillman, 

Washita), Tennessee (only the counties of Carroll, Chester, Crockett, Dyer, Fayette, 

Franklin, Gibson, Hardeman, Hardin, Haywood, Lake, Lauderdale, Lincoln, Madison, 

Obion, Rutherford, Shelby, and Tipton), Texas (except the counties of Carson, Dallam, 

Hansford, Hartley, Hutchinson, Lipscomb, Moore, Ochiltree, Roberts, and Sherman), 

Virginia (only the counties of Dinwiddie, Franklin City, Greensville, Isle of Wight, 

Northampton, Southampton, Suffolk City, Surrey, Sussex), and Missouri (only the 

counties of Dunkin, New Madrid, Pemiscot, Scott, and Stoddard). 
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 b) Post-Harvest Requirements for MON 89034 Sweet Corn 

 Sweet corn is harvested long before field corn. Therefore, if the sweet corn stalks remaining 

in the field and any insects remaining in the stalks are destroyed shortly after harvest, a refuge is 

not needed as a part of the IRM program for sweet corn. Growers must adhere to the following 

types of crop destruction requirements as described in the grower guide/product use guide and/or 

in supplements to the grower guide/product use guide. 

 Crop destruction must occur no later than 30 days following harvest, but preferably 

within 14 days. 

 The allowed crop destruction methods are: rotary mowing, discing, or plow-down. Crop 

destruction methods should destroy any surviving resistant insects. 

 c) Grower Agreements for MON 89034 

1) Persons purchasing MON 89034 must sign a grower agreement. The term “grower 

agreement” refers to any grower purchase contract, license agreement, or similar legal 

document. 

2) The grower agreement and/or specific stewardship documents referenced in the grower 

agreement must clearly set forth the terms of the current IRM program. By signing the 

grower agreement, a grower must be contractually bound to comply with the 

requirements of the IRM program. 

3) Monsanto must integrate this registration into the current system used for their other Bt 

corn PIPS, which is reasonably likely to assure that persons purchasing MON 89034 will 

affirm annually that they are contractually bound to comply with the requirements of the 

IRM program. 

4) Monsanto must continue to use their current grower agreement. If Monsanto wishes to 

change any part of the grower agreement or any specific stewardship documents 

referenced in the grower agreement that would affect either the content of the IRM 

program or the legal enforceability of the provisions of the agreement relating to the IRM 

program, thirty days prior to implementing a proposed change, Monsanto must submit to 

EPA the text of such changes to ensure that it is consistent with the terms and conditions 

of the amendment. 

5) Monsanto must integrate this registration into a current system, which is reasonably 

likely to assure that persons purchasing MON 89034 sign grower agreement(s). 

6) Monsanto shall maintain records of all MON 89034 grower agreements for a period of 

three years from December 31st of the year in which the agreement was signed. 
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7) Beginning on January 31, 2010, and annually thereafter, Monsanto shall provide EPA 

with a report showing the number of units of MON 89034 corn seeds sold or shipped and 

not returned, and the number of such units that were sold to persons who have signed 

grower agreements. The report shall cover the time frame of the twelve-month period 

covering the prior August through July. Note: The first report shall contain the specified 

information from the time frame starting with the date of registration and ending July 31, 

2009. 

8) Monsanto must allow a review of the grower agreements and grower agreement records 

by EPA or by a State pesticide regulatory agency if the State agency can demonstrate that 

confidential business information, including names, personal information, and grower 

license number, will be protected. 

 d) IRM Education and Compliance Monitoring Programs for MON 89034 

1) Monsanto must design and implement a comprehensive, ongoing IRM education program 

designed to convey to MON 89034 users the importance of complying with the IRM 

program. The program shall include information encouraging MON 89034 users to 

pursue optional elements of the IRM program relating to refuge configuration and 

proximity to MON 89034 fields. The education program shall involve the use of multiple 

media, e.g. face-to-face meetings, mailing written materials, EPA-reviewed language on 

IRM requirements on the bag or bag tag, and electronic communications such as by 

Internet, radio, or television commercials. Copies of the materials will be provided to 

EPA for its records. The program shall involve at least one written communication 

annually to each MON 89034 user separate from the grower technical guide. The 

communication shall inform the user of the current IRM requirements. Monsanto shall 

coordinate its education programs with educational efforts of other registrants and other 

organizations, such as the National Corn Growers Association and state extension 

programs. 

2) Annually, Monsanto shall revise, and expand as necessary, its education program to take 

into account the information collected through the compliance survey required under 

paragraphs 6a or 6b and from other sources. The changes shall address aspects of grower 

compliance that are not sufficiently high. 

3) On January 31, 2010, Monsanto must provide a report to EPA summarizing the activities 

carried out under the education program for the prior year. Annually thereafter, 

Monsanto must provide EPA any substantive changes to its grower education activities as 

part of the overall IRM compliance assurance program report. Monsanto must either 

submit a separate report or contribute to the report from the industry working group, 

Agricultural Biotechnology Stewardship Technical Committee (ABSTC). 
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4) Monsanto must design and implement an ongoing IRM compliance assurance program 

designed to evaluate the extent to which growers purchasing MON 89034 are complying 

with the IRM program and that takes such actions as are reasonably needed to assure that 

growers who have not complied with the program either do so in the future or lose their 

access to MON 89034. Monsanto shall coordinate with other Bt corn registrants in 

designing and implementing its compliance assurance program and integrate this 

registration into the current compliance assurance program used for their other Bt corn 

PIPS. Other required features of the program are described in paragraphs 5 – 15 below. 

5) Monsanto must establish and publicize a “phased compliance approach,” i.e., a guidance 

document that indicates how they will address instances of non-compliance with the 

terms of the IRM program and general criteria for choosing among options for 

responding to any non-compliant growers. While recognizing that for reasons of 

difference in business practices there are needs for flexibility between different 

companies, Monsanto must use a consistent set of standards for responding to noncompliance. The options shall include withdrawal of the right to purchase Monsanto corn 

PIP products for an individual grower or for all growers in a specific region. An 

individual grower found to be significantly out of compliance two years in a row would 

be denied sales of Monsanto corn PIP products the next year. Similarly, seed dealers 

who are not fulfilling their obligations to inform/educate growers of their IRM 

obligations will lose their opportunity to sell Monsanto corn PIP products. 

6a) MON 89034 Field Corn: The IRM compliance assurance program shall include an annual 

survey, conducted by an independent third party, of a statistically representative sample of 

growers of MON 89034 field corn who plant the vast majority of all corn in the United States 

and in areas in which the selection intensity is greatest. The survey shall consider only those 

growers who plant 200 or more acres of corn in the Corn-Belt and who plant 100 or more 

acres of corn in corn-cotton areas. The survey shall measure the degree of compliance with 

the IRM program by growers in different regions of the country and consider the potential 

impact of non-response. The sample size and geographical resolution may be adjusted 

annually, based upon input from independent marketing research firms and academic 

scientists, to allow analysis of compliance behavior within regions or between regions. The 

sample size must provide a reasonable sensitivity for comparing results across the United 

States. 

6b) MON 89034 Sweet Corn: The IRM compliance assurance program shall include an 

annual survey of all MON 89034 sweet corn customers who purchase 5 or more bags of 

MON 89034 sweet corn. The survey shall measure the degree of compliance with the IRM 

program, identify the response rate (e.g., the percent of MON 89034 sweet corn acres 

covered by the responses), and consider the potential impact of non-response. An 

independent third party will participate in the design and implementation of the survey. Data 

and information derived from the annual survey will be audited by an independent third 

party. 
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7) The survey shall be designed to provide an understanding of any difficulties growers 

encounter in implementing IRM requirements. An analysis of the survey results must 

include the reasons, extent, and potential biological significance of any implementation 

deviations. 

8) The survey shall be designed to obtain grower feedback on the usefulness of specific 

educational tools and initiatives. 

9a) MON 89034 Field Corn: Monsanto shall provide a final written summary of the results 

of the prior year’s survey (together with a description of the regions, the methodology used, 

and the supporting data) to EPA by January 31st of each year, beginning in 2010. Monsanto 

shall confer with other registrants and EPA on the design and content of the survey prior to 

its implementation. 

9b) MON 89034 Sweet Corn: Monsanto shall provide a written summary of the results of the 

prior year’s survey (together with a description of the methodology used and the supporting 

data) to EPA by January 31st of each year, beginning in 2010. Monsanto shall confer with 

EPA on changes to the design and content of the survey prior to its implementation. 

10) Annually, Monsanto shall revise, and expand as necessary, its compliance assurance 

program to take into account the information collected through the compliance survey 

required under paragraphs 6a through 8 and from other sources. The changes shall address 

aspects of grower compliance that are not sufficiently high. Monsanto must confer with the 

Agency prior to adopting any changes. 

11) Monsanto shall conduct an annual on-farm assessment program. Monsanto shall train its 

representatives who make on-farm visits with growers of MON 89034 to perform 

assessments of compliance with IRM requirements. There is no minimum corn acreage size 

for this program. Therefore, growers will be selected for this program from across all farm 

sizes. In the event that any of these visits result in the identification of a grower who is not in 

compliance with the IRM program, Monsanto shall take appropriate action, consistent with 

its “phased compliance approach,” to promote compliance. 

12) Monsanto shall carry out a program for investigating legitimate “tips and complaints” 

that its growers are not in compliance with the IRM program. Whenever an investigation 

results in the identification of a grower who is not in compliance with the IRM program, 

Monsanto shall take appropriate action, consistent with its “phased compliance approach.” 

13) If a grower, who purchases MON 89034 for planting, was specifically identified as not 

being in compliance during the previous year, Monsanto shall visit with the grower and 

evaluate whether the grower is in compliance with the IRM program for the current year. 
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14) Beginning January 31, 2010 and annually thereafter, Monsanto shall provide a report to 

EPA summarizing the activities carried out under their compliance assurance program for the 

prior year and the plans for the compliance assurance program during the current year. The 

report will include information regarding grower interactions (including, but not limited to, 

on-farm visits, verified tips and complaints, grower meetings and letters), the extent of noncompliance, corrective measures to address the non-compliance, and any follow-up actions 

taken. Monsanto may elect to coordinate information with other registrants and report 

collectively the results of compliance assurance programs. 

15) Monsanto and the seed corn dealers for Monsanto must allow a review of the compliance 

records by EPA or by a State pesticide regulatory agency if the State agency can demonstrate 

that confidential business information, including the names, personal information, and 

grower license number of the growers will be protected. 

e) Insect Resistance Monitoring and Remedial Action Plan for MON 89034 

 The Agency is imposing the following conditions for the Cry1A.105 and Cry2Ab2 toxins 

expressed in MON 89034: 

 Monsanto will monitor for resistance to Cry1A.105 and Cry2Ab2 expressed in MON 89034. 

 

The monitoring program shall consist of two approaches: (1) focused population sampling 

and laboratory testing and (2) investigation of reports of less-than expected control of labeled 

insects. Should field relevant resistance be confirmed, an appropriate resistance management 

action plan will be implemented. 

(1) Focused Population Sampling 

 Monsanto will develop and ensure the implementation of a plan for resistance monitoring for 

Spodoptera frugiperda (fall armyworm or FAW) in counties in which MON 89034 and/or MON 

89034 x MON 88017 sweet corn acreage exceeds 5,000 acres and the pest is capable of 

overwintering in that county. Monsanto should consult with academic and United States 

Department of Agriculture (USDA) experts in developing the monitoring plan and will provide 

EPA with a copy of its proposed resistance monitoring plan for EPA’s approval prior to 

implementation. This proposed FAW monitoring plan must be submitted to EPA by January 31st

of the year following that in which MON 89034 and/or MON 89034 x MON 88017 sweet corn 

acreage exceeds the trigger specified in this requirement (i.e., greater than 5,000 acres in any 

county in which FAW overwinters). The proposed plan must be implemented the season 

following the acreage trigger being met. The proposed plan will remain in place until an EPA 

approved plan can be implemented. 

 

 Monsanto shall annually sample and bioassay populations of the key target pests: Ostrinia 

nubilalis (European corn borer; ECB), Diatraea grandiosella (Southwestern corn borer; SWCB), 
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and Helicoverpa zea (corn earworm; CEW). Sampling for the target pests will be focused in 

areas identified as those with the highest risk of resistance development (e.g., where 

lepidopteran-active Bt hybrids are planted on a high proportion of the corn acres, and where the 

insect species are regarded as key pests of corn). Bioassay methods must be appropriate for the 

goal of detecting field-relevant shifts in population response to MON 89034 and/or changes in 

resistance-allele frequency in response to the use of MON 89034 and, as far as possible, should 

be consistent across sampling years to enable comparisons with historical data. Each protein in 

MON 89034 must be tested separately, rather than a mixture of the two proteins, because 

resistance to one protein could be masked by the activity of the other. 

 The number of populations to be collected shall reflect the regional importance of the insect 

species as a pest, and specific collection regions will be identified for each pest. For ECB, a 

minimum of 12 populations across the sampling region will be targeted for collection at each 

annual sampling. For SWCB, the target will be a minimum of six populations. For CEW, the 

target will be a minimum of 10 populations. Pest populations should be collected from multiple 

corn-growing states reflective of different geographies and agronomic conditions. To obtain 

sufficient sensitivity to detect resistance alleles before they become common enough to cause 

measurable field damage, each population collection shall attempt to target 400 insect genomes 

(egg masses, larvae, mated females, and/or mixed-sex adults), but a successful population 

collection will contain a minimum of 100 genomes. It is recognized that it may not be possible 

to collect the target number of insect populations or genomes due to factors such as natural 

fluctuations in pest density, environmental conditions, and area-wide pest suppression. 

 The sampling program and geographic range of collections may be modified as appropriate 

based on changes in pest importance and for the adoption levels of MON 89034. The Agency 

shall be consulted prior to the implementation of such modifications. 

 The registrant will report to the Agency by August 31st of each year, beginning in 2010, the 

results of the population sampling and bioassay monitoring program. 

 Any incidence of unusually low sensitivity to the Cry1A.105 and Cry2Ab2 proteins in 

bioassays shall be investigated as soon as possible to understand any field relevance of such a 

finding. 

 Such investigations shall proceed in a stepwise manner until the field relevance can be either 

confirmed or refuted, and results of these shall be reported to the Agency annually before August 

31st, beginning in 2010. The investigative steps will include: 

1. Re-test progeny of the collected population to determine whether the unusual bioassay 

response is reproducible and heritable. If it is not reproducible and heritable, no further 

action is required. 

2. If the unusual response is reproducible and heritable, progeny of insects that survive the 
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diagnostic concentration will be tested using methods that are representative of exposure 

to MON 89034 under field conditions. If progeny do not survive to adulthood, any 

suspected resistance is not field relevant and no further action is required. 

3. If insects survive steps 1 and 2, resistance is confirmed, and further steps will be taken to 

taken to evaluate the resistance. These steps may include: 

 determining the nature of the resistance (i.e., recessive or dominant, and the level of 

functional dominance); 

 estimating the resistance-allele frequency in the original population; 

 determining whether the resistance-allele frequency is increasing by analyzing field 

collections in subsequent years sampled from the same site where the resistance allele(s) 

was originally collected; 

 determining the geographic distribution of the resistance allele by analyzing field 

collections in subsequent years from sites surrounding the site where the resistance 

allele(s) was originally collected. 

 Should field relevant resistance be confirmed, and the resistance appears to be increasing or 

spreading, Monsanto will consult with the Agency to develop and implement a case-specific 

resistance management action plan. 

 (2) Investigation of Reports of Unexpected Levels of Damage by the Target Pests: 

 Monsanto will follow up on grower, extension specialist or consultant reports of unexpected 

levels of damage by the lepidopteran pests listed on the pesticide label. Monsanto will instruct 

its customers to contact them if such incidents occur. Monsanto will investigate all legitimate 

reports submitted to the company or the company's representatives. 

 If reports of unexpected levels of damage lead to the suspicion of resistance in any of the key 

target pests (ECB, SWCB, CEW, and FAW), Monsanto will implement the actions described 

below, based on the following definitions of suspected resistance and confirmed resistance. 

 Suspected resistance

 EPA defines suspected resistance to mean field reports of unexpected levels of insect feeding 

damage for which: 

 the corn in question has been confirmed to be lepidopteran-active Bt corn; 
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 the seed used had the proper percentage of corn expressing Bt protein; 

 the relevant plant tissues are expressing the expected level of Bt protein; and 

 it has been ruled out that species not susceptible to the protein could be 

responsible for the damage, that no climatic or cultural reasons could be 

responsible for the damage, and that there could be no other reasonable causes for 

the damage. 

 The Agency does not interpret suspected resistance to mean grower reports of possible 

control failures or suspicious results from annual insect monitoring assays, nor does the Agency 

intend that extensive field studies and testing be undertaken to confirm scientifically the presence 

of insects resistant to MON 89034 in commercial production fields before responsive measures 

are undertaken. 

 If resistance is suspected, Monsanto will instruct growers to do the following: 

 Use alternative control measures in MON 89034 fields in the affected region to 

control the target pest during the immediate growing season. 

 Destroy MON 89034 crop residues in the affected region within one month after 

harvest with a technique appropriate for local production practices to minimize 

the possibility of resistant insects over-wintering and contributing to the next 

season’s target pest population. 

 Additionally, if possible, and prior to the application of alternative control measures or 

destruction of crop residue, Monsanto will collect samples of the insect population in the 

affected fields for laboratory rearing and testing. Such rearing and testing shall be conducted as 

expeditiously as practical. 

 Confirmed resistance 

 EPA defines confirmed resistance to mean, in the case of field reports of unexpected levels 

of damage from the key target pests, that all the following criteria are met: 

 There is >30% insect survival and commensurate insect feeding in a bioassay, 

initiated with neonate larvae, that uses methods that are representative of exposure 

to Bt corn hybrids under field conditions (ECB and SWCB only). 

 In standardized laboratory bioassays using diagnostic concentrations of the Bt

protein suited to the target pest in question, the pest exhibits resistance that has a 

genetic basis and the level of survivorship indicates that there may be a 

resistance-allele frequency of ≥ 0.1 in the sampled population. 
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 In standardized laboratory bioassays, the LC50 exceeds the upper limit of the 95% 

confidence interval of the LC50 for susceptible populations surveyed both in the 

original baselines developed for this pest species and in previous years of field 

monitoring. 

 (3) Response to Confirmed Resistance in a Key Target Pest as the Cause of Unexpected 

Levels of Damage in the Field 

 When field resistance is confirmed (as defined above), the following steps will be taken by 

Monsanto: 

 EPA will receive notification within 30 days of resistance confirmation; 

 Affected customers and extension agents will be notified about confirmed 

resistance within 30 days; 

 Monitoring will be increased in the affected area and local target pest populations 

will be sampled annually to determine the extent and impact of resistance; 

 If appropriate (depending on the resistant pest species, the extent of resistance, the 

timing of resistance, and the nature of resistance, and the availability of suitable 

alternative control measures), alternative control measures will be employed to 

reduce or control target pest populations in the affected area. Alternative control 

measures may include advising customers and extension agents in the affected area 

to incorporate crop residues into the soil following harvest to minimize the 

possibility of over-wintering insects, and/or applications of chemical insecticides; 

 Unless otherwise agreed with EPA, stop sale and distribution of the relevant 

lepidopteran-active Bt corn hybrids in the affected area immediately until an 

effective local mitigation plan approved by EPA has been implemented; 

 Monsanto will develop a case-specific resistance management action plan within 90 

days according to the characteristics of the resistance event and local agronomic 

needs. Monsanto will consult with appropriate stakeholders in the development of 

the action plan, and the details of such a plan shall be approved by EPA prior to 

implementation; 

 Notify affected parties (e.g., growers, consultants, extension agents, seed 

distributors, university cooperators and state/federal authorities as appropriate) in 

the region of the resistance situation and approved action plan; and 
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 In subsequent growing seasons, maintain sales suspension and alternative resistance 

management strategies in the affected region(s) for the Bt corn hybrids that are 

affected by the resistant population until an EPA-approved local resistance 

management plan is in place to mitigate the resistance. 

 A report on results of resistance monitoring and investigations of damage reports must be 

submitted to the Agency annually by August 31st each year, beginning in 2010, for the duration 

of the conditional registration. 

f) Annual Reporting Requirements for MON 89034 

1) Annual Sales: reported and summed by state (county level data available by request), 

January 31st each year, beginning in 2010. 

2) Grower Agreement: number of units of MON 89034 seeds shipped or sold and not 

returned, and the number of such units that were sold to persons who have signed grower 

agreements, January 31st each year, beginning in 2010. 

3) Grower Education: substantive changes to education program completed previous year, 

January 31st each year, beginning in 2010. 

4) Compliance Assurance Plan: Compliance Assurance Program activities and results, 

January 31st each year, beginning in 2010. 

5) Compliance Survey Results: to include annual survey results and plans for the next year; 

full report January 31st each year, beginning in 2010. 

6) Insect Resistance Monitoring Results: results of monitoring and investigations of damage 

reports, August 31st each year, beginning in 2010. 
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B. Data Required to Support MON 89034 x MON 88017 as Amended 

on December 15, 2008: 

1) The subject registration will automatically expire on midnight September 30, 2010. 

2) The subject registration will be limited to MON 89034 x MON 88017 in field or sweet 

corn. Further, MON 89034 x MON 88017 sweet corn may only be sold directly to 

processors or through commercial dealers to large growers. MON 89034 x MON 88017 

sweet corn must not be sold to small roadside or home growers. 

 

3) Submit/cite all data required for registration of your product under FIFRA § 3(c)(5) when 

the Agency requires registrants of similar products to submit such data. 

4) Submit the following data in the time frames listed: 

OPPTS Guideline/ 

Study Type 

Required Data Due Date 

Residue Analytical 

Method – Plants 

(OPPTS 860.1340) 

For event MON 89034 x MON 88017 corn, an independent 

lab validation of the analytical method for the detection of 

Cry2Ab2 and/or Cry1A.105. You must also agree to provide 

to the EPA laboratory (Ft. Meade, MD) methodology and/or 

reagents necessary for validation of such analytical method 

within 6 months from the date that the Agency requests 

them. 

4/1/2009 

Aquatic 

Invertebrate Acute 

Toxicity Testing, 

Freshwater 

Daphnids (OPPTS 

885.4240) 

A 7-14 day Daphnia study as per the 885 Series OPPTS 

Guidelines needs to be performed. Alternatively, a dietary 

study of the effects on an aquatic invertebrate, representing 

the functional group of a leaf shredder in headwater streams, 

can be performed and submitted in lieu of the Daphnia

study. 

9/1/2009* 
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Insect Resistance 

Management – 

Resistance 

Monitoring 

Monsanto must provide additional information on crossresistance of Cry1A.105 and Cry1Ac (preferably including 

binding site models and use of resistant colonies) for the 

target pests and determine how such cross-resistance may 

impact the durability of MON 89034, including any impacts 

in the southern cotton-growing areas. The Cry1A.105 

protein is a chimeric protein consisting of Domains I and II 

and the C-terminus of Cry1Ac. It is important to address not 

only the likelihood of cross-resistance potential of 

Cry1A.105 and Cry1Ab and, similarly, Cry1A.105 and 

Cry2Ab2 (which was done by Monsanto) but also that of 

Cry1A.105 and Cry1Ac. 

7/1/2009* 

Insect Resistance 

Management – 

Resistance 

Monitoring 

Baseline susceptibility studies and/or a discriminating 

concentration assay are required for the Cry1A.105 protein 

against European corn borer (ECB), Southwestern corn borer 

(SWCB), and corn earworm (CEW) and for the Cry2Ab2 

protein against SWCB and CEW. 

7/1/2009* 

For ECB 

and CEW 

8/31/2010* 

For SCWB *

 Extensions were granted as listed in the table above. 

OPPTS Guideline/ 

Study Type 

Required Data Due Date 

Insect Resistance 

Management – 

Resistance 

Monitoring 

To support sweet corn uses, baseline susceptibility studies 

must be conducted on fall armyworm (FAW) populations 

collected from sweet corn growing areas. Monitoring studies 

will be conducted on FAW populations collected from sweet 

corn distribution areas in states in which Monsanto MON 

89034 and/or MON 89034 x MON 88017 sweet corn 

plantings exceed 5,000 acres. The collected populations of 

FAW will be monitored for changes in susceptibility to the 

Cry1A.105 and Cry2Ab2 proteins. 

4/1/2010 

5) Submit or cite all data required to support the individual plant-incorporated protectant in 

Event MON 863 (YieldGard Rootworm), EPA Reg. No. 524-528. In the event that the 

Agency concludes Event MON 863 (YieldGard Rootworm) studies do not sufficiently 

demonstrate a lack of significant adverse effects, additional data with MON 88017 corn 

must be submitted. This data may include a) laboratory toxicity testing with Orius 

insidiosus (minute pirate bug), b) laboratory toxicity testing with a carabid (ground 

beetle), c) long range effects testing on invertebrate populations in the field, and d) long 

range soil persistence testing. 
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6) The Insect Resistance Management (IRM) terms and conditions for this product are as 

follows. 

The required IRM program for MON 89034 x MON 88017 must have the following 

elements: 

 Requirements relating to creation of a non-Bt corn and/or non-lepidopteran 

resistant Bt corn refuge in conjunction with the planting of any acreage of MON 

89034 x MON 88017 field corn; 

 Requirements for Monsanto to prepare and require MON 89034 x MON 88017 

users to sign “grower agreements,” which impose binding contractual obligations on 

the grower to comply with the refuge requirements; 

 Requirements regarding programs to educate growers about IRM requirements; 

 Requirements regarding programs to evaluate and promote growers’ compliance 

with IRM requirements; 

 Requirements regarding programs to evaluate whether there are statistically 

 significant and biologically relevant changes in target insect susceptibility to 

Cry1A.105, Cry2Ab2, and Cry3Bb1 proteins in the target insects; 

 Requirements regarding a “remedial action plan,” which contains measures 

Monsanto would take in the event that any field relevant insect resistance was 

detected as well as to report on activity under the plan to EPA; 

 Submit annual reports on units sold by state (units sold by county level will be 

made available to the Agency upon request), IRM grower agreement results, and the 

compliance assurance program including the education program on or before 

January 31st each year, beginning in 2010. 

a) Refuge Requirements for MON 89034 x MON 88017 Field Corn 

 These refuge requirements do not apply to seed increase/propagation of inbred and hybrid 

seed corn up to a total of 20,000 acres per county and up to a combined United States (U.S.) total 

of 250,000 acres per plant-incorporated protectant (PIP) active ingredient per registrant per year. 

Furthermore, these refuge requirements do not apply to commercial hybrid sweet corn. 
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 1) Corn-Belt Refuge Requirements

 For MON 89034 x MON 88017 field corn grown outside cotton-growing areas (e.g., the 

Corn Belt), grower agreements (also known as stewardship agreements) will specify that growers 

must adhere to the refuge requirements as described in the grower guide/product use guide 

and/or in supplements to the grower guide/product use guide. Two options for the deployment of 

the refuge are available to growers. 

 The first option is planting a common refuge for both corn borers and corn rootworms. The 

common refuge must be planted with corn hybrids that do not contain Bt technologies for the 

control of corn rootworms or corn borers. The refuge area must represent at least 20% of the 

grower’s corn acres (i.e. sum of MON 89034 x MON 88017 acres and refuge acres). It must be 

planted as a block adjacent to the MON 89034 x MON 88017 field, perimeter strips, or in-field 

strips. If perimeter or in-field strips are implemented, the strips must be at least 4 consecutive 

rows wide. The common refuge can be treated with a soil-applied or seed-applied insecticide to 

control rootworm larvae and other soil pests. The refuge can also be treated with a non-Bt foliar 

insecticide for control of late season pests if pest pressure reaches an economic threshold for 

damage (determined using methods recommended by local or regional professionals); however, 

if rootworm adults are present at the time of foliar applications, then the MON 89034 x MON 

88017 field must be treated in a similar manner. 

 The second option is planting separate refuge areas for corn borers and corn rootworms. The 

corn borer refuge must be planted with a non-Bt/lepidopteran-protected hybrid, must represent at 

least 5% of the grower’s corn acres (i.e. sum of MON 89034 x MON 88017 acres and corn borer 

refuge acres), and must be planted within 1⁄2 mile of the MON 89034 x MON 88017 field. 

Refuge planting options include: separate fields, blocks within fields (e.g., along the edges or 

headlands), perimeter strips, or in-field strips. If perimeter or in-field strips are implemented, the 

strips must be at least 4 consecutive rows wide. The corn borer refuge can be treated with a soilapplied or seed-applied insecticide for corn rootworm larval control or a non-Bt foliar-applied 

insecticide for corn borer control if pest pressure reaches an economic threshold for damage 

(determined using methods recommended by local or regional professionals). The corn 

rootworm refuge must be planted with a non-Bt/corn rootworm-protected hybrid but can be 

planted with Bt corn hybrids that control corn borers. The corn rootworm refuge must represent 

at least 20% of the grower’s corn acres (i.e. sum of MON 89034 x MON 88017 acres and corn 

rootworm refuge acres) and must be planted as an adjacent block, perimeter strips, or in-field 

strips. If perimeter or in-field strips are implemented, the strips must be at least 4 consecutive 

rows wide. The corn rootworm refuge can be treated with a soil-applied or seed- applied 

insecticide to control rootworm larvae and other soil pests. The refuge can also be treated with a 

non-Bt foliar insecticide for control of late season pests; however, if rootworm adults are present 

at the time of foliar applications, then the MON 89034 x MON 88017 field must be treated in a 

similar manner. 

 
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 Growers who fail to comply with the IRM requirements risk losing access to Monsanto corn 

PIP Products. 

 2) Cotton-Growing Area Refuge Requirements

 For MON 89034 x MON 88017 field corn grown in cotton-growing areas, grower 

agreements (also known as stewardship agreements) will specify that growers must adhere to the 

refuge requirements as described in the grower guide/product use guide and/or in supplements to 

the grower guide/product use guide. 

 Cotton-growing areas include the following states: Alabama, Arkansas, Georgia, Florida, 

Louisiana, North Carolina, Mississippi, South Carolina, Oklahoma (only the counties of 

Beckham, Caddo, Comanche, Custer, Greer, Harmon, Jackson, Kay, Kiowa, Tillman, Washita), 

Tennessee (only the counties of Carroll, Chester, Crockett, Dyer, Fayette, Franklin, Gibson, 

Hardeman, Hardin, Haywood, Lake, Lauderdale, Lincoln, Madison, Obion, Rutherford, Shelby, 

and Tipton), Texas (except the counties of Carson, Dallam, Hansford, Hartley, Hutchinson, 

Lipscomb, Moore, Ochiltree, Roberts, and Sherman), Virginia (only the counties of Dinwiddie, 

Franklin City, Greensville, Isle of Wight, Northampton, Southampton, Suffolk City, Surrey, 

Sussex), and Missouri (only the counties of Dunkin, New Madrid, Pemiscot, Scott, and 

Stoddard). 

 Two options for the deployment of the refuge are available to growers. 

 The first option is planting a common refuge for both corn borers and corn rootworms. The 

common refuge must be planted with corn hybrids that do not contain Bt technologies for the 

control of corn rootworms or corn borers. The refuge area must represent at least 20% of the 

grower’s corn acres (i.e. sum of MON 89034 x MON 88017 acres and refuge acres). It must be 

planted as a block adjacent to the MON 89034 x MON 88017 field, perimeter strips, or in-field 

strips. If perimeter or in-field strips are implemented, the strips must be at least 4 consecutive 

rows wide. The common refuge can be treated with a soil-applied or seed-applied insecticide to 

control rootworm larvae and other soil pests. The refuge can also be treated with a non-Bt foliar 

insecticide for control of late season pests if pest pressure reaches an economic threshold for 

damage (determined using methods recommended by local or regional professionals); however, 

if rootworm adults are present at the time of foliar applications, then the MON 89034 x MON 

88017 field must be treated in a similar manner. 

 The second option is planting separate refuge areas for corn borers and corn rootworms. The 

corn borer refuge must be planted with a non-Bt/lepidopteran-protected hybrid, must represent at 

least 20% of the grower’s corn acres (i.e. sum of MON 89034 x MON 88017 acres and corn 

borer refuge acres), and must be planted within 1⁄2 mile of the MON 89034 x MON 88017 field. 

 Refuge planting options include: separate fields, blocks within fields (e.g., along the edges or 

headlands), perimeter strips, or in-field strips. If perimeter or in-field strips are implemented, the 
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strips must be at least 4 consecutive rows wide. The corn borer refuge can be treated with a soilapplied or seed-applied insecticide for corn rootworm larval control or a non-Bt foliar-applied 

insecticide for corn borer control if pest pressure reaches an economic threshold for damage 

(determined using methods recommended by local or regional professionals). The corn 

rootworm refuge must be planted with a non-Bt/corn rootworm-protected hybrid but can be 

planted with Bt corn hybrids that control corn borers. The corn rootworm refuge must represent 

at least 20% of the grower’s corn acres (i.e. sum of MON 89034 x MON 88017 acres and corn 

rootworm refuge acres) and must be planted as an adjacent block, perimeter strips, or in-field 

strips. If perimeter or in-field strips are implemented, the strips must be at least 4 consecutive 

rows wide. The corn rootworm refuge can be treated with a soil-applied or seed-applied 

insecticide to control rootworm larvae and other soil pests. The refuge can also be treated with a 

non-Bt foliar insecticide for control of late season pests; however, if rootworm adults are present 

at the time of foliar applications then the MON 89034 x MON 88017 field must be treated in 

a similar manner. 

 Growers who fail to comply with the IRM requirements risk losing access to Monsanto corn 

PIP products. 

 b) Post-Harvest Requirements for MON 89034 x MON 88017 Sweet Corn 

 Sweet corn is harvested long before field corn. Therefore, if the sweet corn stalks remaining 

in the field and any insects remaining in the stalks are destroyed shortly after harvest, a refuge is 

not needed as a part of the IRM program for sweet corn. Growers must adhere to the following 

types of crop destruction requirements as described in the grower guide/product use guide and/or 

in supplements to the grower guide/product use guide. 

 Crop destruction must occur no later than 30 days following harvest, but 

preferably within 14 days. 

 The allowed crop destruction methods are: rotary mowing, discing, or plow down. 

Crop destruction methods should destroy any surviving resistant insects. 

 c) Grower Agreements for MON 89034 x MON 88017 

1) Persons purchasing MON 89034 x MON 88017 must sign a grower agreement. The term 

“grower agreement” refers to any grower purchase contract, license agreement, or similar 

legal document. 

 2) The grower agreement and/or specific stewardship documents referenced in the grower 

agreement must clearly set forth the terms of the current IRM program. By signing the 

grower agreement, a grower must be contractually bound to comply with the 

requirements of the IRM program. 
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3) Monsanto must integrate this registration into the current system used for their other Bt 

corn PIPS, which is reasonably likely to assure that persons purchasing MON 89034 x 

MON 88017 will affirm annually that they are contractually bound to comply with the 

requirements of the IRM program. 

4) Monsanto must continue to use their current grower agreement. If Monsanto wishes to 

change any part of the grower agreement or any specific stewardship documents 

referenced in the grower agreement that would affect either the content of the IRM 

program or the legal enforceability of the provisions of the agreement relating to the IRM 

program, thirty days prior to implementing a proposed change, Monsanto must submit to 

EPA the text of such changes to ensure that it is consistent with the terms and conditions 

of the amendment. 

5) Monsanto must integrate this registration into a current system, which is reasonably 

likely to assure that persons purchasing MON 89034 x MON 88017 sign grower 

agreement(s). 

6) Monsanto shall maintain records of all MON 89034 x MON 88017 grower agreements 

for a period of three years from December 31st of the year in which the agreement was 

signed. 

 

 7) Beginning on January 31, 2010 and annually thereafter, Monsanto shall provide EPA 

with a report showing the number of units of MON 89034 x MON 88017 corn seeds 

sold or shipped and not returned, and the number of such units that were sold to persons 

who have signed grower agreements. The report shall cover the time frame of the 

twelve-month period covering the prior August through July. Note: The first report shall 

contain the specified information from the time frame starting with the date of 

registration and ending July 31, 2009. 

 8) Monsanto must allow a review of the grower agreements and grower agreement records 

by EPA or by a State pesticide regulatory agency if the State agency can demonstrate 

that confidential business information, including names, personal information, and 

grower license number, will be protected. 

 d) IRM Education and Compliance Monitoring Programs for MON 89034 x MON 

88017 

1) Monsanto must design and implement a comprehensive, ongoing IRM education 

program designed to convey to MON 89034 x MON 88017 users the importance of 

complying with the IRM program. The program shall include information encouraging 

MON 89034 x MON 88017 users to pursue optional elements of the IRM program 

relating to refuge configuration and proximity to MON 89034 x MON 88017 fields. The 

education program shall involve the use of multiple media, e.g. face-to-face meetings, 
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mailing written materials, EPA- reviewed language on IRM requirements on the bag or 

bag tag, and electronic communications such as by Internet, radio, or television 

commercials. Copies of the materials will be provided to EPA for its records. The 

program shall involve at least one written communication annually to each MON 89034 

x MON 88017 user separate from the grower technical guide. The communication shall 

inform the user of the current IRM requirements. Monsanto shall coordinate its 

education programs with educational efforts of other registrants and other organizations, 

such as the National Corn Growers Association and state extension programs. 

2) Annually, Monsanto shall revise, and expand as necessary, its education program to take 

into account the information collected through the compliance survey required under 

paragraphs 6a or 6b and from other sources. The changes shall address aspects of grower 

compliance that are not sufficiently high. 

3) On January 31, 2010, Monsanto must provide a report to EPA summarizing the activities 

carried out under the education program for the prior year. Annually thereafter, 

Monsanto must provide EPA any substantive changes to its grower education activities as 

part of the overall IRM compliance assurance program report. Monsanto must either 

submit a separate report or contribute to the report from the industry working group, 

Agricultural Biotechnology Stewardship Technical Committee (ABSTC). 

4) Monsanto must design and implement an ongoing IRM compliance assurance program 

designed to evaluate the extent to which growers purchasing MON 89034 x MON 88017 

are complying with the IRM program and that takes such actions as are reasonably 

needed to assure that growers who have not complied with the program either do so in 

the future or lose their access to MON 89034 x MON 88017. Monsanto shall coordinate 

with other Bt corn registrants in designing and implementing its compliance assurance 

program and integrate this registration into the current compliance assurance program 

used for their other Bt corn PIPS. Other required features of the program are described in 

paragraphs 5 – 15 below. 

5) Monsanto must establish and publicize a “phased compliance approach,” i.e., a guidance 

document that indicates how they will address instances of non-compliance with the 

terms of the IRM program and general criteria for choosing among options for 

responding to any non-compliant growers. While recognizing that for reasons of 

difference in business practices there are needs for flexibility between different 

companies, Monsanto must use a consistent set of standards for responding to noncompliance. The options shall include withdrawal of the right to purchase Monsanto 

corn PIP products for an individual grower or for all growers in a specific region. An 

individual grower found to be significantly out of compliance two years in a row would 

be denied sales of Monsanto corn PIP products the next year. Similarly, seed dealers 

who are not fulfilling their obligations to inform/educate growers of their IRM 

obligations will lose their opportunity to sell Monsanto corn PIP products. 
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6a) MON 89034 x MON 88017 Field Corn: The IRM compliance assurance program shall 

include an annual survey, conducted by an independent third party, of a statistically 

representative sample of growers of MON 89034 x MON 88017 field corn who plant the 

vast majority of all corn in the United States and in areas in which the selection intensity 

is greatest. The survey shall consider only those growers who plant 200 or more acres of 

corn in the Corn-Belt and who plant 100 or more acres of corn in corn-cotton areas. The 

survey shall measure the degree of compliance with the IRM program by growers in 

different regions of the country and consider the potential impact of non-response. The 

sample size and geographical resolution may be adjusted annually, based upon input from 

independent marketing research firms and academic scientists, to allow analysis of 

compliance behavior within regions or between regions. The sample size must provide a 

reasonable sensitivity for comparing results across the United States. 

6b) MON 89034 x MON 88017 Sweet Corn: The IRM compliance assurance program shall 

include an annual survey of all MON 89034 x MON 88017 sweet corn customers who 

purchase 5 or more bags of MON 89034 x MON 88017 sweet corn. The survey shall 

measure the degree of compliance with the IRM program, identify the response rate (e.g., 

the percent of MON 89034 x MON 88017 sweet corn acres covered by the responses), 

and consider the potential impact of non-response. An independent third party will 

participate in the design and implementation of the survey. Data and information derived 

from the annual survey will be audited by an independent third party. 

7) The survey shall be designed to provide an understanding of any difficulties growers 

encounter in implementing IRM requirements. An analysis of the survey results must 

include the reasons, extent, and potential biological significance of any implementation 

deviations. 

8) The survey shall be designed to obtain grower feedback on the usefulness of specific 

educational tools and initiatives. 

9a) MON 89034 x MON 88017 Field Corn: Monsanto shall provide a final written summary 

of the results of the prior year’s survey (together with a description of the regions, the 

methodology used, and the supporting data) to EPA by January 31st of each year, 

beginning in 2010. Monsanto shall confer with other registrants and EPA on the design 

and content of the survey prior to its implementation. 

9b) MON 89034 x MON 88017 Sweet Corn: Monsanto shall provide a written summary of 

the results of the prior year’s survey (together with a description of the methodology 

used and the supporting data) to EPA by January 31st of each year, beginning in 2010. 

Monsanto shall confer with EPA on changes to the design and content of the survey 

prior to its implementation. 
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10) Annually, Monsanto shall revise, and expand as necessary, its compliance assurance 

program to take into account the information collected through the compliance survey 

required under paragraphs 6a through 8 and from other sources. The changes shall 

address aspects of grower compliance that are not sufficiently high. Monsanto must 

confer with the Agency prior to adopting any changes. 

11) Monsanto shall conduct an annual on-farm assessment program. Monsanto shall train 

its representatives who make on-farm visits with growers of MON89034 x MON 88017 

to perform assessments of compliance with IRM requirements. There is no minimum 

corn acreage size for this program. Therefore, growers will be selected for this program 

from across all farm sizes. In the event that any of these visits result in the identification 

of a grower who is not in compliance with the IRM program, Monsanto shall take 

appropriate action, consistent with its “phased compliance approach,” to promote 

compliance. 

12) Monsanto shall carry out a program for investigating legitimate “tips and complaints” 

that its growers are not in compliance with the IRM program. Whenever an 

investigation results in the identification of a grower who is not in compliance with the 

IRM program, Monsanto shall take appropriate action, consistent with its “phased 

compliance approach.” 

13) If a grower, who purchases MON 89034 x MON 88017 for planting, was specifically 

identified as not being in compliance during the previous year, Monsanto shall visit 

with the grower and evaluate whether the grower is in compliance with the IRM 

program for the current year. 

14) Beginning January 31, 2010 and annually thereafter, Monsanto shall provide a report to 

EPA summarizing the activities carried out under their compliance assurance program 

for the prior year and the plans for the compliance assurance program during the 

current year. The report will include information regarding grower interactions 

(including, but not limited to, on-farm visits, verified tips and complaints, grower 

meetings and letters), the extent of non- compliance, corrective measures to address the 

non-compliance, and any follow-up actions 

taken. Monsanto may elect to coordinate information with other registrants and report 

collectively the results of compliance assurance programs. 

15) Monsanto and the seed corn dealers for Monsanto must allow a review of the 

compliance records by EPA or by a State pesticide regulatory agency if the State 

agency can demonstrate that confidential business information, including the names, 

personal information, and grower license number of the growers will be protected. 
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e) Insect Resistance Monitoring and Remedial Action Plans for MON 89034 x MON 

88017 

(1) The Agency is imposing the following conditions for the Cry1A.105 and Cry2Ab2 toxins 

expressed in MON 89034: 

 Monsanto will monitor for resistance to Cry1A.105 and Cry2Ab2 expressed in MON 89034 

x MON 88017. The monitoring program shall consist of two approaches: 

(1) focused population sampling and laboratory testing and (2) investigation of reports of lessthan expected control of labeled insects. Should field relevant resistance be confirmed, an 

appropriate resistance management action plan will be implemented. 

(a) Focused Population Sampling 

 Monsanto will develop and ensure the implementation of a plan for resistance monitoring for 

Spodoptera frugiperda (fall armyworm or FAW) in counties in which MON 89034 and/or MON 

89034 x MON 88017 sweet corn acreage exceeds 5,000 acres and the pest is capable of 

overwintering in that county. Monsanto should consult with academic and United States 

Department of Agriculture (USDA) experts in developing the monitoring plan and will provide 

EPA with a copy of its proposed resistance monitoring plan for EPA’s approval prior to 

implementation. This proposed FAW monitoring plan must be submitted to EPA by January 31st

of the year following that in which MON 89034 and/or MON 89034 x MON 88017 sweet corn 

acreage exceeds the trigger specified in this requirement (i.e., greater than 5,000 acres in any 

county in which FAW overwinters). The proposed plan must be implemented the season 

following the acreage trigger being met. The proposed plan will remain in place until an EPA 

approved plan can be implemented. 

 Monsanto shall annually sample and bioassay populations of the key target pests: Ostrinia 

nubilalis (European corn borer; ECB), Diatraea grandiosella (Southwestern corn borer; SWCB), 

and Helicoverpa zea (corn earworm; CEW). Sampling for the target pests will be focused in 

areas identified as those with the highest risk of resistance development (e.g., where 

lepidopteran-active Bt hybrids are planted on a high proportion of the corn acres, and where the 

insect species are regarded as key pests of corn). Bioassay methods must be appropriate for the 

goal of detecting field-relevant shifts in population response to MON 89034 x MON 88017 

and/or changes in resistance-allele frequency in response to the use of MON 89034 x MON 

88017 and, as far as possible, should be consistent across sampling years to enable comparisons 

with historical data. Each protein in MON 89034 must be tested separately, rather than a mixture 

of the two proteins, because resistance to one protein could be masked by the activity of the 

other. 

 The number of populations to be collected shall reflect the regional importance of the insect 

species as a pest, and specific collection regions will be identified for each pest. For ECB, a 

minimum of 12 populations across the sampling region will be targeted for collection at each 
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annual sampling. For SWCB, the target will be a minimum of six populations. For CEW, the 

target will be a minimum of 10 populations. Pest populations should be collected from multiple 

corn-growing states reflective of different geographies and agronomic conditions. To obtain 

sufficient sensitivity to detect resistance alleles before they become common enough to cause 

measurable field damage, each population collection shall attempt to target 400 insect genomes 

(egg masses, larvae, mated females, and/or mixed-sex adults), but a successful population 

collection will contain a minimum of 100 genomes. It is recognized that it may not be possible 

to collect the target number of insect populations or genomes due to factors such as natural 

fluctuations in pest density, environmental conditions, and area-wide pest suppression. 

 The sampling program and geographic range of collections may be modified as appropriate 

based on changes in pest importance and for the adoption levels of MON 89034 x MON 88017. 

The Agency shall be consulted prior to the implementation of such modifications. 

 The registrant will report to the Agency by August 31st of each year, beginning in 2010, the 

results of the population sampling and bioassay monitoring program. 

 Any incidence of unusually low sensitivity to the Cry1A.105 and Cry2Ab2 proteins in 

bioassay shall be investigated as soon as possible to understand any field relevance of such a 

finding. Such investigations shall proceed in a stepwise manner until the field relevance can be 

either confirmed or refuted, and results of these shall be reported to the Agency annually before 

August 31st, beginning in 2010. The investigative steps will include: 

1. Re-test progeny of the collected population to determine whether the unusual bioassay 

response is reproducible and heritable. If it is not reproducible and heritable, no further 

action is required. 

2. If the unusual response is reproducible and heritable, progeny of insects that survive the 

diagnostic concentration will be tested using methods that are representative of exposure 

to MON 89034 x MON 88017 under field conditions. If progeny do not survive to 

adulthood, any suspected resistance is not field relevant and no further action is required. 

3. If insects survive steps 1 and 2, resistance is confirmed, and further steps will be taken to 

taken to evaluate the resistance. These steps may include: 

 determining the nature of the resistance (i.e., recessive or dominant, and the level of 

functional dominance); 

 estimating the resistance-allele frequency in the original population; 

 determining whether the resistance-allele frequency is increasing by analyzing field 

collections in subsequent years sampled from the same site where the resistance 

allele(s) was originally collected; 
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 determining the geographic distribution of the resistance allele by analyzing field 

collections in subsequent years from sites surrounding the site where the resistance 

allele(s) was originally collected. 

 Should field relevant resistance be confirmed, and the resistance appears to be increasing or 

spreading, Monsanto will consult with the Agency to develop and implement a case-specific 

resistance management action plan. 

 (b) Investigation of Reports of Unexpected Levels of Damage by the Target Pests: 

 Monsanto will follow up on grower, extension specialist or consultant reports of unexpected 

levels of damage by the lepidopteran pests listed on the pesticide label. Monsanto will instruct 

its customers to contact them if such incidents occur. Monsanto will investigate all legitimate 

reports submitted to the company or the company's representatives. 

 If reports of unexpected levels of damage lead to the suspicion of resistance in any of the key 

target pests (ECB, SWCB, CEW, and FAW), Monsanto will implement the actions described 

below, based on the following definitions of suspected resistance and confirmed resistance.

 Suspected resistance

 EPA defines suspected resistance to mean field reports of unexpected levels of insect feeding 

damage for which: 

 the corn in question has been confirmed to be lepidopteran-active Bt corn; 

 the seed used had the proper percentage of corn expressing Bt protein; 

 the relevant plant tissues are expressing the expected level of Bt protein; and 

 it has been ruled out that species not susceptible to the protein could be 

responsible for the damage, that no climatic or cultural reasons could be 

responsible for the damage, and that there could be no other reasonable causes for 

the damage. 

 The Agency does not interpret suspected resistance to mean grower reports of possible 

control failures or suspicious results from annual insect monitoring assays, nor does the Agency 

intend that extensive field studies and testing be undertaken to confirm scientifically the 

presence of insects resistant to MON 89034 x MON 88017 in commercial production fields 

before responsive measures are undertaken. 
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 If resistance is suspected, Monsanto will instruct growers to do the following: 

 Use alternative control measures in MON 89034 x MON 88017 fields in the 

affected region to control the target pest during the immediate growing season. 

 Destroy MON 89034 x MON 88017 crop residues in the affected region within 

one month after harvest with a technique appropriate for local production 

practices to minimize the possibility of resistant insects over-wintering and 

contributing to the next season’s target pest population. 

 Additionally, if possible, and prior to the application of alternative control measures or 

destruction of crop residue, Monsanto will collect samples of the insect population in the 

affected fields for laboratory rearing and testing. Such rearing and testing shall be conducted as 

expeditiously as practical. 

 Confirmed resistance 

 EPA defines confirmed resistance to mean, in the case of field reports of unexpected levels 

of damage from the key target pests, that all the following criteria are met: 

 There is >30% insect survival and commensurate insect feeding in a bioassay, 

initiated with neonate larvae, that uses methods that are representative of exposure 

to Bt corn hybrids under field conditions (ECB and SWCB only) . 

 In standardized laboratory bioassays using diagnostic concentrations of the Bt

protein suited to the target pest in question, the pest exhibits resistance that has a 

genetic basis and the level of survivorship indicates that there may be a 

resistance-allele frequency of ≥ 0.1 in the sampled population. 

 In standardized laboratory bioassays, the LC50 exceeds the upper limit of the 95% 

confidence interval of the LC50 for susceptible populations surveyed both in the 

original baselines developed for this pest species and in previous years of field 

monitoring. 

 (c) Response to Confirmed Resistance in a Key Target Pest as the Cause of Unexpected 

Levels of Damage in the Field 

 When field resistance is confirmed (as defined above), the following steps will be taken by 

Monsanto: 

 EPA will receive notification within 30 days of resistance confirmation; 
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 Affected customers and extension agents will be notified about confirmed 

resistance within 30 days; 

 Monitoring will be increased in the affected area and local target pest populations 

will be sampled annually to determine the extent and impact of resistance; 

 If appropriate (depending on the resistant pest species, the extent of resistance, the 

timing of resistance, and the nature of resistance, and the availability of suitable 

alternative control measures), alternative control measures will be employed to 

reduce or control target pest populations in the affected area. Alternative control 

measures may include advising customers and extension agents in the affected 

area to incorporate crop residues into the soil following harvest to minimize 

the possibility of over-wintering insects, and/or applications of chemical 

insecticides; 

 Unless otherwise agreed with EPA, stop sale and distribution of the relevant 

lepidopteran-active Bt corn hybrids in the affected area immediately until an 

effective local mitigation plan approved by EPA has been implemented; 

 Monsanto will develop a case-specific resistance management action plan within 

90 days according to the characteristics of the resistance event and local 

agronomic needs. Monsanto will consult with appropriate stakeholders in the 

development of the action plan, and the details of such a plan shall be approved 

by EPA prior to implementation; 

 Notify affected parties (e.g., growers, consultants, extension agents, seed 

distributors, university cooperators and state/federal authorities as appropriate) in 

the region of the resistance situation and approved action plan; and 

 In subsequent growing seasons, maintain sales suspension and alternative 

resistance management strategies in the affected region(s) for the Bt corn hybrids 

that are affected by the resistant population until an EPA-approved local 

resistance management plan is in place to mitigate the resistance. 

 A report on results of resistance monitoring and investigations of damage reports must be 

submitted to the Agency annually by August 31st each year, beginning in 2010, for the duration 

of the conditional registration. 

(2) The Agency is imposing the following conditions for the Cry3Bb1 toxin expressed in 

MON 88017: 
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i) Monsanto must monitor for Cry3Bb1 resistance and/or trends in increased tolerance for 

corn rootworm utilizing the current corn rootworm resistance monitoring plan for MON 

89034 x MON 88017. Sampling should be focused in those areas in which there is the 

highest risk of resistance development. 

ii) Monsanto must develop and validate an appropriate discriminating or diagnostic dose 

assay by January 31, 2010. 

iii) Monsanto must finalize rootworm damage guidelines and submit these to EPA by 

January 31, 2010. 

 iv) Monsanto must follow-up on grower, extension specialist or consultant reports of 

 unexpected damage or control failures for corn rootworm. 

v) Monsanto must provide EPA with an annual resistance monitoring report by August 31st

each year, beginning with 2010, reporting on populations collected the previous year. 

vi) The current remedial action plan approved for MON 863 must be used for corn rootworm 

suspected and confirmed resistance in MON 89034 x MON 88017. If corn rootworm 

resistance is confirmed, all acres of MON 89034 x MON 88017 and refuges must be 

treated with insecticides targeted at corn rootworm adults as well as larvae. 

f) Annual Reporting Requirements for Mon 89034 x MON 88017 

1) Annual Sales: reported and summed by state (county level data available by request), 

January 31st each year, beginning in 2010; 

2) Grower Agreement: number of units of MON 89034 x MON 88017 seeds shipped or sold 

and not returned, and the number of such units that were sold to persons who have signed 

grower agreements, January 31st each year, beginning in 2010; 

3) Grower Education: substantive changes to education program completed previous year, 

January 31st each year, beginning in 2010; 

4) Compliance Assurance Plan: Compliance Assurance Program activities and results, 

January 31st each year, beginning in 2010; 

5) Compliance Survey Results: to include annual survey results and plans for the next year; 

full report January 31st each year, beginning in 2010; 

6) Insect Resistance Monitoring Results: results of monitoring and investigations of damage 

reports, August 31st each year, beginning in 2010. 
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IV. REGULATORY POSITION FOR Cry1A.105, AND Cry2Ab2 

Pursuant to FIFRA section 3(c)(7)(C), EPA may conditionally register a new pesticide active 

ingredient for a period of time reasonably sufficient for the generation and submission of 

required data that are lacking because insufficient time has elapsed since the imposition of the 

data requirement for those data to be developed. EPA may grant such conditional registration 

only if EPA determines that (1) the use of the pesticide product during the period of the 

conditional registration will not cause any unreasonable adverse effect on the environment, and 

(2) the registration and use of the pesticide during the conditional registration is in the public 

interest. EPA determines that all of these criteria have been fulfilled. 

The first criterion under FIFRA Section 3(c)(7)(C) mentioned above has been met because 

insufficient time has elapsed since the imposition of the data requirements for: 

1) an independent lab validation of the analytical method for the detection of Cry2Ab2 and/or 

Cry1A.105 to satisfy residue analytical method in plants requirements for event MON 

89034 corn and event MON 89034 x MON 88017 corn, 

2) a 7 to 14 day Daphnia study as per the 885 Series OPPTS Guidelines or alternatively, a 

dietary study of the effects on an aquatic invertebrate, representing the functional group of 

a leaf shredder in headwater streams.. 

3) additional information on cross-resistance of Cry1A.105 and Cry1Fa and Cry1Ac 

(preferably including binding site models and use of resistant colonies) for the target pests 

and determine how such cross-resistance may impact the durability of MON 89034 

4) baseline susceptibility studies and/or a discriminating concentration assay that are required 

for the Cry1A.105 protein against ECB, SWCB, and CEW and for the Cry2Ab2 protein 

against SWCB, CEW. 

5) baseline susceptibility studies to support sweet corn uses that must be conducted on FAW 

populations collected from sweet corn growing areas; Monitoring studies that will be 

conducted on FAW populations collected from sweet corn distribution areas in states in 

which Monsanto MON 89034 and/or MON 89034 x MON 88017 sweet corn plantings 

exceed 1000 acres; and monitoring of the collected populations of FAW for changes in 

susceptibility to the Cry1A.105 and Cry2Ab2 proteins. 

The applicants submitted or cited data sufficient for EPA to determine that conditional 

registration of Bacillus thuringiensis Cry2Ab2 and Cry 1A.105 proteins and the genetic material 

necessary for their production in event MON 89034 field corn and sweet corn under FIFRA 

3(c)(7)(C) will not result in unreasonable adverse effects to the environment, as discussed above. 

The applicants submitted and/or cited satisfactory data pertaining to the proposed use. The 

human health effects data and nontarget organism effects data are considered sufficient for the 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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period of the conditional registration. These data demonstrate that no foreseeable human health 

hazards or ecological effects are likely to arise from the use of the product and that the risk of 

resistance developing to Cry2Ab2 and Cry 1A.105 proteins, during the conditional registrations 

are not expected to be significant. 

Registration of Bacillus thuringiensis Cry2Ab2 and Cry 1A.105 proteins and the genetic material 

necessary for their production in event MON 89034 field corn and sweet corn is in the public 

interest because: 

(1) Registration of MON 89034 is expected to result in the reduction of the use of higher risk, 

and often less effective and more expensive, conventional pesticides. A reduction in use of 

conventional pesticides equates to less potential for adverse effects to human health and the 

environment. 

(2) Additionally, MON 89034 provide a wider spectrum of protection against primary and 

secondary corn pests, which should facilitate greater grain quality, a reduction of mycotoxin 

contamination, increased yield and ultimately have positive implications for human health. 

In view of these minimal risks and the clear benefits related to Bacillus thuringiensis Cry2Ab2 

and Cry 1A.105 proteins and the genetic material necessary for their production in event MON 

89034 field corn and sweet corn , EPA believes that the use of the product during the limited 

period of the conditional registration will not cause any unreasonable adverse effects. 

Although the data with respect to this particular new active ingredient are satisfactory, they are 

not sufficient to support an unconditional registration under FIFRA 3(c)(5). Additional data are 

necessary to evaluate the risk posed by the continued use of this product. Consequently, EPA is 

imposing the data requirements specified earlier in Section III. 

EPA has determined, as explained in section II.F., that the third criterion for a FIFRA 3(c)(7)(C) 

conditional registration has been fulfilled because the use of Bacillus thuringiensis Cry2Ab2 and 

Cry 1A.105 proteins and the genetic material necessary for their production in event MON 

89034 field corn and sweet corn under this registration is in the public interest. 

The submitted data in support of this registration under section 3(c)(7)(C) of the Federal 

Insecticide, Fungicide, and Rodenticide Act (FIFRA) have been reviewed and determined to be 

adequate. Studies mentioned above are included in the terms, conditions, and limitations of these 

registrations. This registration will not cause unreasonable adverse effects to man or the 

environment and is in the public interest. 

The expiration date of the registrations has been set to September 30, 2010. 
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V. BIBLIOGRAPHY: 

Citations Considered as part of the Data Base Supporting the Registration of MON 89034 

MRID: 46471600 

Citation: Monsanto Co. (2005) Submission of Product Chemistry and Residue Data in Support 

of the Experimental Use Permit of Bacillus thuringiensis in ZMIR245 Corn. Transmittal of 1 

Study. 

MRID: 46471601 

Citation: Bogdanova, N.; Brown, S.; Huber, S. (2005) Volume 2: Data in Support of an 

Application for an Experimental Use Permit to Test ZMIR245 x MON 88017 Combined Trait 

Corn Along with ZMIR245 and MON 88017. Project Number: 04/CR/130E. Unpublished study 

prepared by Monsanto Company. 15 p. 

MRID: 46694603 

Citation: Bonnette, K. (2005) Am Acute Oral Toxicity Study in Mice with Cry1A.105 Protein: 

Final Report. Project Number: EUF00081, CRO/2005/050. Unpublished study prepared by 

Charles River Laboratories, Inc. 119 p. 

MRID: 46694605 

Citation: McCoy, R.; Silvanovich, A. (2005) Bioinformatics Analysis of the Cry1A.105 Protein 

Utilizing the AD5, Toxin5, and Allpeptides Databases: Final Report. Project Number: 19686, 

05/01/62/01. Unpublished study prepared by Monsanto Co. 549 p. 

MRID: 46694606 

Citation: Kapadia, S.; Rice, E. (2005) Assessment of the In Vitro Digestibility of the CryA.105 

Protein in Simulated Gastric Fluid: Final Report. Project Number: MSL/19929, 05/01/62/02. 

Unpublished study prepared by Monsanto Company. 23 p. 

MRID: 46694607 

Citation: Goley, M.; Thorp, J. (2005) Immunodetection of Cry2Ab2 and Cry1A.105 Proteins in 

Corn Grain from MON 89034 Following Heat Treatment: Final Report. Project Number: 

MSL/19899, 05/01/39/27. Unpublished study prepared by Monsanto Co. 38 p. 

MRID: 46874901 

Citation: ABSTC. 2006. Monitoring the Susceptibility of Corn Lepidoptera Pests to CrylAb and 

Cry1F Proteins: 2005 Monitoring Results. Report submitted to EPA by the IRM Technical 

Subcommittee of the Agricultural Biotechnology Stewardship Technical Committee. 

MRID: 46951300 

Citation: Monsanto Company (2006) Submission of Product Chemistry, Efficacy, Exposure and 

Risk Data in Support of the Application for Registration of MON 89034 x 88017. Transmittal of 

6 Studies. 
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MRID: 46951301 

Citation: Bogdanova, N. (2006) Human Health and Environmental Assessment of the PlantIncorporated Protected Bacillus thuringiensis Cry1A.105, Cry2Ab2 and Cry3Bb1 Proteins 

Produced in Insect-Protected Corn MON 89034 x MON 88017. Project Number: MSL/20434. 

Unpublished study prepared by Monsanto Company. 27 p. 

MRID: 46951302 

Citation: Groat, J.; Wolff, B.; Rice, J.; et. al. (2006) Confirmation of the Integrity of Corn MON 

89034 x MON 88017 by Southern Blot Analysis. Project Number: 06/01/50/03, MSL/20145. 

Unpublished study prepared by Monsanto Company. 22 p. 

MRID: 46951303 

Citation: Hartmann, A.; Niemeyer, K.; Silvanovich, A. (2006) Assessment of the Cry1A.105, 

Cry2Ab2, Cry3Bb1, and CP4 EPSPS Protein Levels in Selected Tissues of Insect-Protected Corn 

MON 89034 x MON 88017 Produced in 2005 U.S. Field Trials. Project Number: MSL0020479. 

Unpublished study prepared by Monsanto Company. 41 p. 

MRID: 46951304 

Citation: Levine, S. L.; Camp, R.; Uffman, J.; Vaughan, T.; et. al. (2006) An Evaluation of the 

Insect Bioefficacy of Combined Trait Products Produced Through Conventional Breeding: MON 

89034 x NK603 and MON 89034 x MON 88017. Project Number: 06/01/50/02, MSL/20336. 

Unpublished study prepared by Monsanto Company. 24 p. 

MRID: 46951305 

Citation: MacRae, T.; Brown, C.; Levine, S. (2006) Evaluation of Potential for Interactions 

Between the Bacillus thuringensis Proteins Cry1A.105, Cry2Ab2, and Cry3Bb1. Project 

Number: 05/01/39/19, MSL/20270. Unpublished study prepared by Monsanto Company. 26 p. 

MRID: 46951306 

Citation: Head, G. (2006) Insect Resistance Management Plan for the Combined Trait Product 

MOON 89034 x MON 88017. Project Number: 06/RA/50/03. Unpublished study prepared by 

Monsanto Company. 18 p. 

MRID: 46951400 

Citation: Monsanto Company (2006) Submission of Efficay, Toxicity, Residue, Environmental 

Fate, Exposure and Risk Data in Support of the Application for Registration of MON 89034. 

Transmittal of 30 Studies. 
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MRID: 46951401 

Citation: Bogdanova, N. (2006) Human Health and Environmental Assessment of the PlantIncorporated Protectant Bacillus thuringiensis Cry1A.105 and Cry2Ab2 Proteins Produced in 

Corn MON 89034. Project Number: MSL/20405. Unpublished study prepared by Monsanto 

Company. 88 p. 

MRID: 46951402 

Citation: Rice, J.; Wolff, B.; Groat, J.; et. al. (2006) Amended Report for MSL-20072: 

Molecular Analysis of Corn MON 89034. Project Number: 05/01/39/12, MSL/20311. 

Unpublished study prepared by Monsanto Company. 67 p. 

MRID: 46951403 

Citation: Hartmann, A.; Niemeyer, K.; Silvanovich, A. (2006) Assessment of the Cry1A.105 

and Cry2Ab2 Protein Levels in Tissues of Insect-Protected Corn MON 89034 Produced in 2005 

U.S. Field Trials. Project Number: 05/01/39/32, MSL/20285. Unpublished study prepared by 

Monsanto Company. 31 p. 

MRID: 46951404 

Citation: Karunanandaa, K.; Thorp, J.; Goley, M.; et al. (2006) Characterization of the Cry2Ab2 

Protein Purified from the Corn Grain of MON 89034 and Comparison of the Physicochemical 

and Functional Properties of the Plant-Produced and E. coli-Produced Cry2Ab2 Proteins. Project 

Number: 20071, 60/100075. Unpublished study prepared by Monsanto Company. 55 p. 

MRID: 46951405 

Citation: Levine, S.; Uffman, J. (2006) Evaluation of the Functional Equivalence of the 

Cry2Ab2 Protein Produced in E. coli and Bt Against a Sensitive Lepidopteran Species. Project 

Number: 05/01/39/23, MSL/20132. Unpublished study prepared by Monsanto Company. 23 p. 

MRID: 46951406 

Citation: Bonnette, K. (2006) An Acute Oral Toxicity Study in Mice with Cry2Ab2 Protein: 

Final Report. Project Number: CRO/2005/049, EUF00080/ MSL/19901. Unpublished study 

prepared by Charles River Laboratories, Inc. 113 p. 

MRID: 46951407 

Citation: Kapadia, S.; Rice, E. (2006) Assessment of the in vitro Digestibility of the Cry2Ab2 

Protein in Simulated Gastric Fluid. Project Number: MSL/19931, 05/01/62/04. Unpublished 

study prepared by Monsanto Company. 23 p. 

MRID: 46951408 

Citation: Kapadia, S.; Rice, E. (2005) Assessment of the in vitro Digestibility of the Cry1A.105 

Protein in Simulated Intestinal Fluid. Project Number: MSL/19930, 05/01/62/03. Unpublished 

study prepared by Monsanto Company. 20 p. 
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MRID: 46951409 

Citation: Thorp, J.; Goley, M. (2006) Assessment of the in vitro Digestibility of the Cry2Ab2 

Protein in Simulated Intestinal Fluid. Project Number: MSL/19938, 05/01/62/05. Unpublished 

study prepared by Monsanto Company. 20 p. 

MRID: 46951410 

Citation: McClain, J.; Silvanovich, A. (2006) Bioinformatics Evaluation of the Cry1A.105 

Protein Utilizing the AD6, TOXIN5, and ALLPEPTIDES Databases. Project Number: 20351, 

06/01/62/04. Unpublished study prepared by Monsanto Company. 550 p. 

MRID: 46951411 

Citation: McClain, J.; Silvanovich, A. (2006) Bioinformatics Analysis of the Cry2Ab2 Protein 

Utilizing the AD6, TOXIN5, and ALLPEPTIDES Databases. Project Number: 20307, 

06/01/62/01. Unpublished study prepared by Monsanto Company. 361 p. 

MRID: 46951412 

Citation: Davis, S. (2006) Comparison of Broiler Performance and Carcass Parameters When 

Fed Diets Containing MON 89034, Control or Commercial Corn: Amended Final Report. 

Project Number: MN/05/2, 05/01/50/13. Unpublished study prepared by Colorado Quality 

Research and University of Missouri. 96 p. 

MRID: 46951413 

Citation: Macrae, T.; Brown, C.; Levine, S. (2006) Spectrum of Insecticidal Activity of Bacillus 

thuringiensis Cry1A.105 Protein. Project Number: 04/01/39/27, MSL/20230. Unpublished 

study prepared by Monsanto Company. 18 p. 

MRID: 46951414 

Citation: Macrae, T.; Brown, C.; Levine, S. (2006) Spectrum of Insecticidal Activity of Bacillus 

thuringensis Cry2Ab2 Protein. Project Number: 05/01/62/07, MSL/20229. Unpublished study 

prepared by Monsanto Company. 17 p. 

MRID: 46951415 

Citation: Headrick, J.; Heredia, O.; Oyediran, I.; et. al. (2006) Assessment of the Efficacy of 

Lepidopteran-protected Corn MON 89034 and MON 89597 Against Major Insect Pest in the 

United States, Puerto Rico and Argentina During 2003-2004 Seasons. Project Number: 

05/RA/39/05. Unpublished study prepared by Monsanto Company. 18 p. 

MRID: 46951416 

Citation: Teixeira, D. (2006) Evaluation of Dietary Effects of Lyophilized Leaf Tissue from 

Corn MON 89034 in a Chronic Exposure Study with Collembola (Folsomia candida). Project 

Number: 252/6197, SE/2005/027, MSL/20169. Unpublished study prepared by Springborn 

Smithers Laboratories. 42 p. 
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MRID: 46951417 

Citation: Palmer, S.; Krueger, H. (2006) Evaluation of Exposure to MON 89034 with the 

Cladoceran Daphnia magna: An Acute Static-Renewal Test with Corn Pollen: Final Report. 

Project Number: 139A/330, WL/2005/011. Unpublished study prepared by Wildlife 

International, Ltd. 32 p. 

MRID: 46951418 

Citation: Sindermann, A.; Porch, J.; Krueger, H. (2006) Evaluation of Potential Effects of 

Exposure to Cry1A.105 Protein in an Acute Study with the Earthworm in an Artificial Soil 

Substrate: Final Report. Project Number: 139/472, WL/2005/068, MSL/20147. Unpublished 

study prepared by Wildlife International, Ltd. 48 p. 

MRID: 46951419 

Citation: Richards, K. (2006) Evaluation of the Dietary Effect(s) of a Cry1A.105 Protein on 

Honeybee Larvae (Apis mellifera L.). Project Number: CAR/128/05, CA/2005/071. 

Unpublished study prepared by California Agricultural Research Inc. 43 p. 

MRID: 46951420 

Citation: Richards, K. (2006) Evaluation of the Dietary Effect(s) of a Cry1A.105 Protein on 

Adult Honeybees (Apis mellifera L.). Project Number: CAR/129/05, CA/2005/072. 

Unpublished study prepared by California Agricultural Research Inc. 52 p. 

MRID: 46951421 

Citation: Paradise, M.; Jiang, C.; Duan, J.; et. al. (2006) Evaluation of Potential Dietary Effects 

of Cry1A.105 Protein on the Ladybird Beetle, Coleomegilla maculata (Coleoptera: 

Coccinellidae). Project Number: 05/01/39/24, MSL/20150. Unpublished study prepared by 

Monsanto Company. 41 p. 

MRID: 46951422 

Citation: Paradise, M.; Jiang, C.; Duan, J.; et. al. (2006) Evaluation of Potential Dietary Effects 

of Cry2Ab2 Protein on the Ladybird Beetle, Coleomegilla maculata (Coleoptera: Coccinellidae). 

Project Number: 05/01/39/25, MSL/20151. Unpublished study prepared by Monsanto Company. 

42 p. 

MRID: 46951423 

Citation: Teixeira, D. (2006) Evaluation of Potential Dietary Effects of Cry1A.105 Protein on 

Minute Pirate Bugs, Orius insidiosus (Hemiptera: Anthocoridae). Project Number: 252/6201, 

SE/2005/074, MSL/20170. Unpublished study prepared by Springborn Smithers Laboratories. 

105 p. 
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MRID: 46951424 

Citation: Teixeira, D. (2006) Evaluation of Potential Dietary Effects of Cry2Ab2 Protein on 

Minute Pirate Bugs, Orius insidiosus (Hemiptera: Anthocoridae). Project Number: 252/6200, 

SE/2005/075, MSL/20171. Unpublished study prepared by Springborn Smithers Laboratories. 

57 p. 

MRID: 46951425 

Citation: Sindermann, A.; Porch, J.; Krueger, H. (2006) Evaluation of Potential Effects of 

Exposure to Cry1A.105 Protein in an Acute Study with the Parasitic Wasp, Ichneumon 

promissorius (Hymenoptera: Ichneumonidae). Project Number: 139/475, WL/2005/128, 

MSL/20149. Unpublished study prepared by Wildlife International, Ltd. 47 p. 

MRID: 46951426 

Citation: Sindermann, A.; Porch, J.; Krueger, H. (2006) Evaluation of Potential Effects of 

Exposure to Cry2Ab2 Protein in an Acute Study with the Parasitic Wasp, Ichneumon 

promissorius (Hymenoptera: Ichneumonidae). Project Number: 139/476, WL/2005/129, 

MSL/20148. Unpublished study prepared by Wildlife International, Ltd. 47 p. 

MRID: 46951427 

Citation: Gallagher, S.; Beavers, J. (2006) Evaluation of Potential Dietary Effects of MON 

89034 with the Northern Bobwhite: and Eight-Day Dietary Study with Corn Grain. Project 

Number: 139/470, WL/2005/012. Unpublished study prepared by Wildlife International, Ltd. 

60 p. 

MRID: 46951428 

Citation: Mueth, M.; Curran, T.; Warren, J.; et. al. (2006) Aerobic Soil Degradation of the 

Purified Cry2Ab2 and Cry1A.105 Proteins. Project Number: 05/01/39/29, MSL/20174. 

Unpublished study prepared by Monsanto Company, Agvise Inc. and University of Nebraska. 

83 p. 

MRID: 46951429 

Citation: Huesing, J.; Duan, J.; Levine, S. (2006) Endangered Species Risk Assessment for Corn 

MON 89034. Project Number: MSL0020394. Unpublished study prepared by Monsanto 

Company. 24 p. 

MRID: 46951430 

Citation: Head, G. (2006) Insect Resistance Management Plan for Second Generation 

Lepidopteran-Protected Corn, MON 89034. Project Number: 06/RA/39/06. Unpublished study 

prepared by Monsanto Company. 98 p. 

MRID: 47079400 

Citation: Monsanto Co. (2007) Submission of Efficacy Data in Support of the Application for 

Registration of MON 89034 X 88017. Transmittal of 2 Studies. 
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MRID: 47079402 

Citation: Bogdanova, N. (2007) Supplemental Information to Address EPA Questions 

Regarding Applications 524-LTL and 524-LTA to Register Insect-protected Corn MON 89034 

and MON 89034 x MON 88017 (MRID 46951400 and 46951300). Project Number: 

04/C4/172/2. Unpublished study prepared by Monsanto Co. 16 p. 

MRID: 47079403 

Citation: Headrick, J.; Heredia, O.; Oyediran, I. (2006) Assessment of the Efficacy of InsectProtected Corn MON 89034, MON 89034 x NK603, and MON 89034 x MON 88017 Against 

Major Insect Pests in the Field Trials Conducted in U.S. During 2005. Project Number: 

05/RA/50/04. Unpublished study prepared by Monsanto Co. 19 p. 

MRID: 47127500 

Citation: Monsanto Company (2007) Submission of Product Chemistry and Toxicity Data in 

Support of the Application for Registration of MON 89034 and MON 89034 x MON 88017. 

Transmittal of 5 Studies. 

MRID: 47127501 

Citation: Bogdanova, N. (2007) Responses to EPA Questions Regarding Applications 524-LTL 

and 524-LTA to Register Insect-protected Corn MON 89034 and MON 89034 X MON 88017 

(MRID 46951400 and 46951300). Project Number: 04/CR/172/3, 04/CR/172E/3. Unpublished 

study prepared by Monsanto Co. 10 p. 

MRID: 47127502 

Citation: Groat, J.; Wolff, B.; Rice, J.; et al. (2007) Confirmation of the Integrity of Corn MON 

89034 X MON 88017 by Southern Blot Analysis. Project Number: 06/CR/172/3, 

BR/ME/0878/01, BR/ME/0094/01. Unpublished study prepared by Monsanto Company. 69 p. 

MRID: 47127503 

Citation: Rice, J.; Wolff, B.; Groat, J.; et al. (2006) Amended Report for MSL-20072: Molecular 

Analysis of Corn MON 89034. Project Number: 06/CR/172/3, BR/ME/0878/01, 

BR/ME/0094/01. Unpublished study prepared by Monsanto Company. 78 p. 

MRID: 47127504 

Citation: Hartmann, A.; Niemeyer, K.; Silvanovich, A. (2006) Assessment of Cry1A.105, 

Cry2Ab2, Cry3Bb1 and CP4 EPSPS protein Levels in Selected Tissues of INsect-protected Corn 

MON 89034 x MON 88017 Produced in 2005 U.S. Field Trials. Project Number: 06/CR/172/3, 

BR/ME/0197/05, BR/ME/0197. Unpublished study prepared by Monsanto Company. 93 p. 

MRID: 47127505 

Citation: Hartmann, A.; Niemeyer, K.; Silvanovich, A. (2006) Assessment of the Cry1A.105 

and Cry2Ab2 Protein Levels in Tissues of Insect-protected Corn MON 89034 Produced in 2005 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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U.S. Field Trials. Project Number: 06/CR/172/3, AG/EQ/1023/01, BR/ME/1026/01. 

Unpublished study prepared by Monsanto Company. 51 p. 

MRID: 47140300 

Citation: Monsanto Company (2007) Submission of Exposure and Risk Data in Support of the 

Application for Registration of MON 89034. Transmittal of 1 Study. 

MRID: 47140301 

Citation: Bogdanova, N.; Dubelman, S.; Mueth, M.; et al. (2007) Responses to EPA Questions 

Regarding Application 521-LTL to Register Insect-Protected Corn MON 89034 (MRID 

46951428). Project Number: 04/CR/172/4. Unpublished study prepared by Monsanto 

Agricultural Co. 9 p. 

MRID: 47166401 

Citation: Ali, I.; Luttrell, R.; Abel, C. (2007) Monitoring Bt Susceptibilities in Helicoverpa zea: 

Results of 2006 Studies. Project Number: 04/CT/133E/31, 04/CT/134E/31. Unpublished study 

prepared by USDA, ARS Southern Insect Management Laboratory and University of Arkansas. 

23 p. 

MRID: 47166403 

Citation: Ali, I.; Luttrell, R.; Abel, C. (2007) Monitoring Bt Susceptibilities in Helicoverpa zea 

and Heliothis virescens: Results of 2006 Studies. Project Number: 04/CT/133E/31, 

04/CT/134E/31. Unpublished study prepared by University of Arkansas and USDA, ARS 

Southern Insect Management Laboratory. 16 p. 

MRID: 47243700 

Citation: Monsanto Company (2007) Submission of Residue Data in Support of the 

Experimental Use of Bacillus thuringiensis Cry1A.105 and Cry2Ab2 Proteins and the Genetic 

Materials Necessary for its Production in Corn. Transmittal of 4 Studies. 

MRID: 47243701 

Citation: Murphy, J.; Stillwell, L. (2007) Cry1A.105, Cry2Ab2, Cry3Bb1, and CP4 EPSPS 

Protein Levels in Selected Tissues of the Combined Trait Corn Product MON 89034 x TC 1507 

x MON 88017 x DAS-59122-7, in Support of an Application for an Experimental Use Permit. 

Project Number: 07/CR/185E/2. Unpublished study prepared by Monsanto Company. 13 p. 

MRID: 47243702 

Citation: Phillips, A. (2007) Cry34Ab1, Cry35Ab1, Cry1F and PAT Protein Levels in Selected 

Tissues of the Combined-Trait Corn Product MON 89034 x TC 1507 x MON 88017 x DAS59122-7, in Support of an Application for an Experimental Use Permit: Interim Report. Project 

Number: 061026/01. Unpublished study prepared by Dow AgroSciences LLC. 15 p. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

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158

MRID: 47243703 

Citation: Burns, J. (2007) Petition to Amend 40 CFR 174.502 to Establish a Temporary 

Exemption from the Requirement of a Tolerance for Bacillus thuringiensis Cry1A.105 

Insecticidal Protein and the Gentic Material Necessary for its Production When Used as a PlantIncorporated Protectant in Food and Feed Commodities of Field Corn, Sweet Corn, and Popcorn, 

to Support an EUP. Project Number: 07/CR/185E/3. Unpublished study prepared by Monsanto 

Company. 19 p. 

MRID: 47243704 

Citation: Burns, J. (2007) Petition to Amend 40 CFR 174.503 to Establish a Temporary 

Exemption from the Requirement of a Tolerance for Bacillus thuringiensis Cry2Ab2 Insecticidal 

Protein and the Genetic Material Necessary for its Production When Used as a PlantIncorporated Protectant in Food and Feed Commodities of Field Corn, Sweet Corn, and Popcorn, 

to Support an EUP. Project Number: 07/CR/185E/4. Unpublished study prepared by Monsanto 

Company. 18 p. 

MRID: 47279700 

Citation: Monsanto Company (2007) Submission of Public Interest Data in Support of the 

Applications for Registration of MON 89034 and MON 89034 x MON 88017. Transmittal of 1 

Study. 

MRID: 47279701 

Citation: Crawford, A.; Bogdanova, N. (2007) Public Interest Document for MON 89034 and 

MON 89034 x MON 88017. Project Number 07/RA/50/04, 07/RA/39/04. Unpublished study. 

112 p. 

MRID: 47444911 

Schlenz, M.L., Babcock, J.M., and Storer, N.P., 2008. Response of Cry1F-resistant and 

susceptible European corn borer and fall armyworm colonies to Cry1A.105 and Cry2Ab2. 

Attached as Appendix 1 of the Insect Resistance Management Plan for MON 89034 x TC1507 x 

MON 88017 x DAS-59122-7. 

MRID: 47474801 

Citation: Gustafson, D. I. and Head, G.P., 2008. Modeling the Impact of a Five-Percent 

Structured Refuge on the Evolution of European and Southwestern Corn Borer Resistance to 

MON 89034 Corn. Report submitted to EPA by Monsanto. 

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Additional Benefits Assessment References:

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submitted by Monsanto for MON 88017 corn. A. Reynolds and S. Matten memo to M. 

Mendelsohn, 10/13/2005. 

BPPD, 2007a. Review of Monsanto Company’s assessment of the efficacy of lepidopteranprotected corn MON 89034 and MON 89597 during the 2003 and 2004 seasons. S. Matten 

memo to S. Cerrelli, 10/30/2007. 

BPPD, 2007b. Review of proposed insect resistance management plan submitted by Monsanto 

for MON 89034 x MON 88017 Bt corn. A. Reynolds memo to S. Cerrelli, 11/29/2007. 

BPPD, 2007c. Product performance of Bt Cry1A.105 protein (MRID 469514-13). Data 

Evaluation Record from E. Lewis to S. Matten, 07/23/2007. 

BPPD, 2007d. Product performance of Bt Cry2Ab2 protein (MRID 469514-14). Data Evaluation 

Record from E. Lewis to S. Matten, 07/20/2007. 
Bacillus thuringiensis Cry1A.105, and Cry2Ab2 Protein in Corn 

Biopesticide Registration Action Document 

168

BPPD, 2007e. Review of human health and product characterization data for registration of B. 

thuringiensis Cry1A.105 and Cry2Ab2 proteins and the genetic material necessary for their 

production in MON 89034 corn. R. Edelstein memo to S. Cerrelli, 11/07/2007. 

BPPD, 2007f. Review of “Evaluation of Potential Interactions between the Bacillus thuringiensis

Proteins Cry1A.105, Cry2Ab2, and Cry3Bb1” for Monsanto’s MON 89034 x MON 88017 

maize. M. Hunter memo to S. Cerrelli, 12/14/2007. 

BPPD, 2007g. Cry3Bb1 Corn Biopesticide Registration Action Document. Available at: 

http://www.epa.gov/oppbppd1/biopesticides/ingredients/tech_docs/brad_006484.htm 

BPPD, 2007h. Technical review of Monsanto Company’s submissions regarding insect 

resistance management for MON 89034 corn. S. Matten and A. Reynolds memo to S. Cerrelli, 

11/06/2007. 

BPPD, 2007i. Review of non-target invertebrate studies for MON 89034 maize containing 

Cry1A.105 and Cry2Ab2 proteins. M. Hunter memo to S. Cerrelli, 12/14/2007. 

BPPD, 2007j. Review of broiler, quail, soil fate, and endangered species data packages submitted 

in support of MON 89034 Bt corn, T. Milofsky to S. Cerrelli, 12/14/2007. 

Crawford, A. and Bogdanova, N., 2007. Public interest document supporting registration of 

Bacillus thuringiensis Cry1A.105, Cry2Ab2 and Cry3Bb1 proteins and the genetic material 

necessary for their productions in insect-protected corn MON 89034 and MON 89034 x MON 

88017. Report submitted by Monsanto Company MRID 472797-01. 

Rosi-Marshall E.J., J.L. Tank, T.V. Royer, M.R. Whiles, M. Evans-White, C. Chambers, N.A. 

Griffiths, J. Pokelsek, and M.L. Stephen. (2007). Toxins in transgenic crop byproducts may 

affect headwater stream ecosystems. PNAS, vol. 104, no. 41, 16204–16208. 

Wu, F. (2008). Field Evidence: Bt Corn and Mycotoxin Reduction. Available at: 

http://www.isb.vt.edu/news/2008/feb08.pdf