# Patent

## Title
Method for cultivating plant

## Abstract

A plant-cultivating method is provided which comprises a step (A) of irradiating a plant with a red light and a step (B) of irradiating a plant with a blue light, wherein the step (A) and the step (B) are independently carried out for a predetermined period of time under cultivation conditions such that a fertilizer is used for each of the step (A) and the step (B), of which at least the fertilizer used for the step (A) is applied in the form of a nutritious liquid containing fertilizer ingredients and further carbon dioxide added therein. Preferably, a nutritious liquid is applied at each of the step (A) and the step (B), and the nutritious liquid applied at the step (A) contains carbon dioxide at a concentration higher than that in the nutritious liquid applied at the step (B).

## Background
BACKGROUND OF THE INVENTION
(1) Field of the Invention
This invention relates to a method for cultivating a plant. More particularly it relates to a method for cultivating a plant while the plant is irradiated with an artificial light using a light-emitting lamp for plant cultivation whereby growth of the plant is promoted.
(2) Description of Related Art
Light-emitting technology has heretofore been adopted for growing seedlings or promoting growth of plants whereby a cultivation period of plants can be shortened and frequency of harvesting in the same farm can be increased. Plants can be grown to a large size within a predetermined period of time, and the crop yield can be enhanced.
As a plant cultivation technique utilizing artificial light irradiation, an illumination lamp facility for irradiating a plant alternately with green light and white light has been proposed, for example, in patent document 1. In this illumination lamp facility, plants are irradiated alternately with green light having a wavelength of 500 to 570 nm and white light having a wavelength of 300 to 800 nm whereby simulated day and night are created. Consequently the sugar translocation within plant bodies is smoothly effected and growth of plants is enhanced.
Another proposal has been proposed in patent document 2, which comprises an illumination lamp facility equipped with a light emitting diode (LED) for irradiating plants alternately or concurrently with blue light having a wavelength of 400-480 nm and red light having a wavelength of 620-700 nm to supply light energy for cultivation, growth, and tissue cultivation of plants. This illumination lamp facility is characterized as irradiating plants selectively with light having a wavelength corresponding to the light absorption peak of chlorophyll, i.e., in the vicinity of 450 nm and the vicinity of 660 nm whereby the plants are cultivated with an enhanced energy efficiency.
It is stipulated in patent document 2 that blue light and red light may be irradiated either concurrently or alternately (see patent document 2, claim 1). More specifically it is described in this patent document that single radiation of blue light, single radiation of red light and concurrent radiation of blue light and red light are compared with each other, and it was verified that the concurrent radiation of blue light and red light exhibited an enhanced effect on healthy growth of plants, which is similar to the growth achieved by sun light radiation, whereas the single radiation of blue light or red light brings about unhealthy growth such as spindly growth of plants (see patent document 2, paragraph [0011]). It is further described in patent document 2 that blue light and red light are alternately radiated by blinking by means of a blinking pattern at a high frequency of several megahertz or more (see patent document 2, paragraph [0006]).
However, patent document 2 is silent on a method of alternately conducting a blue light irradiation step and a red light irradiation step, and thus, growth promoting effects achieved by the alternate light irradiation method are not verified.
Patent document 3 has proposed a plant cultivation technique utilizing a solution culture having nanobubbles of carbon dioxide incorporated therein to enhance the promotion of photosynthesis of plants.
PRIOR ART DOCUMENTS
Patent Documents
Patent document 1: JP 1994-276858A
Patent document 2: JP 1996-103167A
Patent document 3: JP 2011-110028A
To enhance the productivity in plant cultivation, a plant cultivation method utilizing artificial light irradiation, which is simple and easy, and exhibits enhanced energy effect and excellent growth promotion effect, is eagerly desired.
BRIEF SUMMARY OF THE INVENTION
A primary object of the present invention is to provide an improved method of cultivating a plant using an artificial light-irradiating facility, whereby high value-added and highly nutritious plants can be produced with improved growth promotion effect.
The present inventors made extensive research for providing an improved method for cultivating a plant using an artificial light-irradiating lamp facility with enhanced growth promotion effect, and have found that a plant-cultivation method comprising a step (A) of irradiating a plant with a red light and a step (B) of irradiating the plant with a blue light, wherein the two steps (A) and (B) are independently carried out for a predetermined period of time, exhibits an enhanced growth promotion effect, and further found that the growth promotion effect varies depending upon the cultivation conditions, especially the concentration of carbon dioxide in a liquid fertilizer. An excellent growth promotion effect has been obtained by using a nutritious liquid having carbon dioxide added therein, especially a nutritious liquid containing nanobubbles of carbon dioxide added therein. Based on these findings, the present invention has been completed.
In accordance with the present invention, there are provided the following plant-cultivating methods.
(1). A method of cultivating a plant comprising a step (A) of irradiating a plant with a red light and a step (B) of irradiating a plant with a blue light, wherein the red light irradiation step (A) and the blue light irradiation step (B) are independently carried out for a predetermined period of time under cultivation conditions such that a fertilizer is used at each of the red light irradiation step (A) and the blue light irradiation step (B), of which at least the fertilizer used at the red light irradiation step (A) is applied in the form of a nutritious liquid containing fertilizer ingredients and further carbon dioxide added therein.
(2). The method of cultivating a plant as mentioned above in (1), wherein a nutritious liquid containing fertilizer ingredients is applied at each of the red light irradiation step (A) and the blue light irradiation step (B), and the nutritious liquid applied at the red light irradiation step (A) contains carbon dioxide at a concentration higher than the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B).
(3). The method of cultivating a plant as mentioned above in (2), wherein the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) is in the range of 1,000 ppm to 5,000 ppm, and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is in the range of 500 ppm to 2,000 ppm.
(4). The method of cultivating a plant as mentioned above in (2), wherein the difference between the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is at least 300 ppm.
(5). The method of cultivating a plant as mentioned above in (2), wherein the difference between the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is in the range of 500 ppm to 1,000 ppm.
(6). The method of cultivating a plant as mentioned above in (2), wherein, among the nutritious liquids applied at the red light irradiation step (A) and the blue light irradiation step (B), at least the nutritious liquid applied at the red light irradiation step (A) contains carbon dioxide added therein by bubbling carbon dioxide, or by incorporating therein carbonated water containing carbon dioxide added by bubbling.
(7). The method of cultivating a plant as mentioned above in (2), wherein, among the nutritious liquids applied at the red light irradiation step (A) and the blue light irradiation step (B), at least the nutritious liquid applied at the red light irradiation step (A) contains nanobubbles of carbon dioxide which are prepared by bubbling carbon dioxide therein.
(8). The method of cultivating a plant as mentioned above in (2), wherein the nutritious liquids applied at the red light irradiation step (A) and at the blue light irradiation step (B) contain nitrogen, phosphorus and potassium as fertilizer ingredients, and, a content of at least one of the fertilizer ingredients in the nutritious liquid applied at the red light irradiation step (A) is larger than the content of the corresponding fertilizer ingredient in the nutritious liquid applied at the blue light irradiation step (B).
(9). The method of cultivating a plant as mentioned above in (1), wherein the plant is selected from algae, phytoplankton and euglena.
(10). The method of cultivating a plant as mentioned above in (1), wherein the red light-irradiation step (A) and the blue light irradiation step (B) are alternately and repeatedly carried out over a period of at least one hour for each irradiation time.
(11). The method of cultivating a plant as mentioned above in (1), wherein the red light irradiation step (A) and the blue light irradiation step (B) are carried out using an illumination lamp facility having red light emitting elements and blue light emitting elements, both of which are capable of being independently operated, and the red light emitting elements and the blue light emitting elements exhibit a light emission intensity ratio of red light to blue light of at least 1:1 as expressed by a ratio of photosynthetic photon flux density of red light to blue light.
(12). The method of cultivating a plant as mentioned above in (1), wherein the red light irradiation step (A) and the blue light irradiation step (B) are carried out using an illumination lamp facility having red light emitting elements and blue light emitting elements, both of which are capable of being independently operated, and the red light emitting elements and the blue light emitting elements exhibit a light emission intensity ratio of red light to blue light in the range of 2:1 to 9:1 as expressed by a ratio of photosynthetic photon flux density of red light to blue light.
By the term “plant(s)” as used in this specification, we mean plants in a broad sense which include leaf plants, fruit plants, grains and algae. The plant cultivation method according to the present invention exhibits especially marked growth promotion effect for phytoplankton such as algae, and mosses and euglena.
By independently carrying out the red light irradiation step (A) and the blue light irradiation step (B), and further by using a nutritious liquid containing fertilizer ingredients and further carbon dioxide added therein, at least at the red light irradiation step (A) according to the present invention, high value-added and highly nutritious plants can be produced with improved growth promotion effect.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a perspective view of an example of an illumination lamp having red light emitting elements and blue light emitting elements as used in the plant-cultivation method of the present invention.



DETAILED DESCRIPTION OF THE INVENTION
Preferred modes for practicing the invention will be described with reference to the accompanying drawings. The described modes by no means limit the scope of the invention.
The plant cultivation method of the present invention is characterized by comprising a step (A) of irradiating the plant with a red light and a step (B) of irradiating the plant with a blue light, wherein the red light irradiation step (A) and the blue light irradiation step (B) are independently carried out for a predetermined period of time under cultivation conditions such that a fertilizer is used at each of the red light irradiation step (A) and the blue light irradiation step (B), of which at least the fertilizer used at the red light irradiation step (A) is applied in the form of a nutritious liquid containing fertilizer ingredients and further carbon dioxide added therein.
Chlorophyll in mesophyll cells performs photosynthesis, that is, chlorophyll produces oxygen and saccharides from carbon dioxide in the air, absorbed through stomata of leaves, and from water, absorbed through roots. It is well known that the growth of plants greatly varies depending upon the photosynthesis. It is also known that the photosynthesis of plants is promoted by using a nutritious liquid having nanobubbles of carbon dioxide added therein, as described in patent document 3.
When red light and blue light are irradiated on a plant, the light absorption curve of chlorophyll has different peaks attributed to the red light and the blue light. Thus the red light and the blue light exhibit different functions. The red light is concerned with activation of phytochrome, and the blue light is concerned with activation of phototropin and cryptochrome. The present inventors have investigated the difference between the functions of red light and blue light in the growth of plants, and found that red light tends to enlarge the area of plant leaves, and blue light tends to be concerned with morphogenesis of plants. The mechanism on which red light and blue light have the different functions is not clear, but it is presumed that red light and blue light exhibit different chemical reactions with regard to the photosynthesis reaction.
In the plant-cultivation method of the present invention, the red light irradiation step (A) and the blue light irradiation step (B) are independently carried out for a predetermined period of time. By carrying out separately the red light irradiation and the blue light irradiation, the desired plant growth promoting effect can be attained. If the red light irradiation and the blue light irradiation are concurrently carried out, the photosynthesis processes by the red light irradiation and the blue light irradiation interfere with each other and do not proceed effectively.
It was further found that when an aquaculture of plants is carried out using a nutritious liquid having carbon dioxide added therein, high value-added and highly nutritious plants can be produced with improved growth promotion effect by appropriately controlling the light irradiating conditions and the concentration of carbon dioxide. Especially the light irradiating conditions and the concentration of carbon dioxide give a great influence on the content of calcium in the plants.
In the plant-cultivation method according to the present invention, wherein a nutritious liquid containing carbon dioxide added therein is applied at least at the red light irradiation step (A), it is preferable that a nutritious liquid is applied at each of the red light irradiation step (A) and the blue light irradiation step (B), and further that the nutritious liquid applied at the red light irradiation step (A) contains carbon dioxide at a concentration higher than the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B).
More specifically it is preferable that the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) is in the range of 1,000 ppm to 5,000 ppm, and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is in the range of 500 ppm to 2,000 ppm. The reason for which the optimum concentrations of carbon dioxide are different between the nutritious liquid applied at the red light irradiation step (A) and the nutritious liquid applied at the blue light irradiation step (B) is not clear, but it is presumed that the reaction in the process of photosynthesis and the reactions in other processes occurring at the two light irradiation steps are closely related to the plant cultivation conditions.
The difference between the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is preferably at least 300 ppm, and more preferably in the range of 500 ppm to 1,000 ppm.
Among the nutritious liquids applied at the red light irradiation step (A) and the blue light irradiation step (B), at least the nutritious liquid applied at the red light irradiation step (A) is more preferably a nutritious liquid containing carbon dioxide incorporated therein by bubbling carbon dioxide, or by adding therein carbonated water having incorporated carbon dioxide by bubbling.
The concentration of carbon dioxide in the nutritious liquid can be voluntarily controlled by the conventional procedure. By adopting a nanobubble technique as developed in recent years, the concentration of carbon dioxide can be voluntarily controlled and increased more easily. Therefore, it is especially preferable that the nutritious liquid applied at the red light irradiation step (A) or both of the nutritious liquid applied at the red light irradiation step (A) and the nutritious liquid applied at the blue light irradiation step (B) are a nutritious liquid containing nanobubbles of carbon dioxide prepared by bubbling carbon dioxide therein.
Nanobubbles are fine bubbles having a diameter of 10 nm to 300 nm. Most conventional as-prepared bubbles are easily float on the water surface and disappear. In contrast, nanobubbles have unique properties such as long lifetime in water, that is, they are only slightly affected by buoyancy and exist as they are in water for a long period of time.
By using a nutritious liquid containing an increased amount of carbon dioxide, carbon dioxide and ionic ingredients, contained in the nutritious liquid, can be absorbed effectively over a long period of time. Especially calcium ingredients contained in the nutritious liquid can be absorbed with a high efficiency.
In the plant-cultivation method of the present invention, a nutritious liquid is applied at least at the red light irradiation step (A) as a fertilizer constituting the growing conditions. That is, a nutritious liquid is applied only at the red light irradiation step (A), or preferably at both of the red light irradiation step (A) and the blue light irradiating step (B).
Three primary nutrients, i.e., nitrogen, phosphorus and potassium, are essential for the growth of plants. Nitrogen takes part in proliferation and growth of cells, and thus, indispensable for growth of plants. Phosphorus is an ingredient of nucleic acid in plants, and has a role of promoting growth of plants. Potassium has a role of promoting growth of plant roots. Other nutrients such as minerals including, for example, calcium, magnesium and iron are preferably contained in a nutritious liquid.
As specific examples of the fertilizer as used in the present invention, there can be mentioned nitrogen fertilizers such as ammonium sulfate, ammonium chloride, ammonium nitrate, urea, nitrogenous lime, potassium nitrate, calcium nitrate and sodium nitrate; phosphate fertilizers such as superphosphate of lime and fused magnesium phosphate; potassium fertilizers such as potassium chloride and potassium sulfate; and minerals such as calcium, magnesium and iron.
The content of the three primary nutrients, i.e., nitrogen, phosphorus and potassium, in the nutritious liquid is not particularly limited, but, it is preferable that both of the nutritious liquids as applied at the red light irradiation step (A) and at the blue light irradiating step (B) contain nitrogen, phosphorus and potassium as fertilizer ingredients, and further that a content of at least one nutrient (fertilizer ingredient) in the nutritious liquid applied at the red light irradiation step (A) is larger than the content of the corresponding fertilizer ingredient in the nutritious liquid applied at the blue light irradiation step (B). More preferably, contents of the three fertilizer ingredients in the nutritious liquid applied at the red light irradiation step (A) are larger than the contents of the corresponding three fertilizer ingredients in the nutritious liquid applied at the blue light irradiation step (B).
More specifically, the nutritious liquid applied at the red light irradiation step (A) preferably contains 15-20 me/L of nitrogen, 3-6 me/L of phosphorus and 6-9 me/L of potassium, and the nutritious liquid applied at the blue red light irradiation step (B) preferably contains 10-15 me/L of nitrogen, 1-4 me/L of phosphorus and 2-6 me/L of potassium.
Illumination Lamp for Plant Cultivation
In the plant cultivating method of the present invention, an illumination lamp facility for plant cultivation having red light emitting elements and blue light emitting elements, both of which are capable of being independently operated, is used for independently (preferably, alternately and repeatedly) carrying out the red light irradiation step (A) and the blue light irradiation step (B) for a predetermined period of time.
FIG. 1 is a perspective view of a preferable example of an illumination lamp for plant cultivation which has red light emitting elements and blue light emitting elements. This illumination lamp 1 has a light irradiation part 11 having a rectangular shape in the planar view, and further has a control part (not shown) for controlling the light irradiation part 11.
As illustrated in FIG. 1, the light irradiation part 11 is provided with plural red light emitting elements 2 and plural blue light emitting elements 3. In the plant cultivation lamp 1, as specifically illustrated in FIG. 1, the ratio in number of red light emitting elements 2 to blue light emitting elements 3 is 2:1.
Plural red light emitting elements 2 are arranged with equal intervals along a straight line extending in the length direction of the light irradiation part 11, and plural blue light emitting elements 3 are also arranged in a similar manner. The straight line of red light emitting elements 2 and the straight line of blue light emitting elements 3 are substantially parallel to each other.
The number of red light emitting elements 2 and the number of blue light emitting elements 3 may be the same or different in the illumination lamp 1, although the ratio in number of red light emitting elements to blue light emitting elements in the lamp illustrated in FIG. 1 is 2:1.
The rate of plant growth sometimes varies depending upon the light emission intensity ratio of red light and blue light. For example, some plants exhibit enhanced rate of growth when the red light emission intensity is larger than the blue light emission intensity. For these plants, it is preferable to use a plant cultivating illumination lamp 1 having a light emitting part provided with a larger number of red light emitting elements 2 than blue light emitting elements 3. By using the lamp 1 provided with a larger number of red light emitting elements 2 than blue light emitting elements 3, the light emission intensity of red light can be easily made larger than that of blue light.
For many plants, the light emission intensity of red light is preferably larger than that of blue light. Practically, for these plants, the light emission intensity ratio of red light to blue light (i.e., a ratio of the total emission intensity of red light to the total emission intensity of blue light) is preferably at least 1:1, more preferably in the range of 2:1 to 9:1, and especially preferably in the range of 2:1 to 5:1. It is preferable to use an illumination lamp having a light emitting part provided with a larger number of red light emitting elements than the number of blue light emitting elements, which part gives output beam having a light emission intensity ratio in the above-mentioned range when an appropriate current is applied to the respective red light emitting elements and blue light emitting elements.
The illumination lamp is preferably such that an applied electrical current can be exactly adjusted and the ratio of light emission intensity of red light to that of blue light can be desirably varied depending upon the particular kind of plant by precisely adjusting the electrical current.
If the ratio of light emission intensity of red light to that of blue light is smaller than the above-specified range, i.e., the blue light emission intensity is too large as compared with the red light emission intensity, the desired rate of growth is often difficult to attain. In contrast, if the ratio of red light emission intensity to blue light emission intensity is larger than the above-specified range, i.e., the red light emission intensity is too large, the desired rate of plant growth is also often difficult to attain, and, for example, undesirable growth such as spindly growth or overgrowth tends to occur.
The light irradiation part of the illumination lamp facility as used in the present invention preferably has a mixed light emitting package such that red light emitting elements and blue light emitting elements are arranged in a single light emitting package. The mixed light emitting package preferably has a function such that the emission intensity of red light emitting elements and the emission intensity of blue light emitting elements are capable of being independently controlled.
The ratio of the total emission intensity of red light to the total emission intensity of blue light in the mixed light emitting package is preferably at least 1:1, more preferably in the range of 2:1 to 9:1, and especially preferably in the range of 2:1 to 5:1. By providing the light irradiation part with such mixed light emitting package, the red light emitting elements and the blue light emitting elements can be arranged with enhanced density in the light irradiation part.
Conventional light emitting elements can be used as the red light emitting elements 2 and blue light emitting elements 3. For example, light emitting diode (LED) in which the desired wavelength can be easily selected and light energy within the effective wavelength region occupies a predominant part can be beneficially used. Laser diode (LD) and an electroluminescent (EL) element can also be used. The EL element used may be either an organic EL element or inorganic EL element. Light emitting diode is most preferable because of low cost and high emission efficiency.
Red light emitted from the red light emitting elements 2 has a wavelength in the range of 570 nm to 730 nm. Preferably the red light has a center emission wavelength within the range of 645 nm to 680 nm, and a wavelength in the range of the center emission wavelength ±50 nm, more preferably the center emission wavelength ±30 nm, and especially preferably the center emission wavelength ±10 nm.
Blue light emitted from the blue light emitting elements 3 has a wavelength in the range of 400 nm to 515 nm. Preferably the red light has a center emission wavelength within the range of 420 nm to 490 nm, and a wavelength in the range of the center emission wavelength 450±30 nm, more preferably the center emission wavelength 450±20 nm, and especially preferably the center emission wavelength 450±10 nm.
The red light irradiated to a plant at the red light irradiating step (A) may include a minor proportion of lights other than red light, for example, blue light, provided that the total irradiated light exhibits a red light emission intensity ratio of at least 60%. According to the research of the present inventors, enhancement of plant growth at the red light irradiation step (A) can be observed when the total irradiated light exhibits a blue light emission intensity ratio of up to approximately 30%. The total irradiated light should preferably exhibit a blue light emission intensity ratio of up to approximately 20%, and most preferably zero. An example of the red light irradiated to a plant at the red light irradiating step (A) exhibits a red light emission intensity ratio of 60%, a far-infrared light emission intensity ratio of 20% and a blue light emission intensity ratio of 20%. Most preferable example of the red light exhibits a red light emission intensity ratio of 100%.
The blue light irradiated to a plant at the blue light irradiating step (B) may include a minor proportion of lights other than blue light, for example, red light, provided that the total irradiated light exhibits a blue light emission intensity ratio of at least 60%. According to the research of the present inventors, enhancement of plant growth at the blue light irradiation step (B) can be observed when the total irradiated light exhibits a red light emission intensity of up to approximately 30%. The total irradiated light should preferably exhibit a red light emission intensity ratio of up to approximately 20%, and most preferably zero. An example of the red light irradiated to a plant at the blue light irradiating step (B) exhibits a blue light emission intensity ratio of 60%, a far-infrared light emission intensity ratio of 20% and a red light emission intensity ratio of 20%. Most preferable example of the blue light exhibits a blue light emission intensity ratio of 100%.
By the term “light emission intensity ratio” as used in the specification, we mean those which are expressed by photosynthetic photon flux density (PPFD; in μmol/m2s).
The red light and the blue light, irradiated from the light irradiation part 11, preferably exhibit a photosynthetic photon flux density in the range of approximately 1 to 1,000 μmol/m2s, more preferably approximately 10 to 500 μmol/m2s and especially preferably approximately 50 to 250 μmol/m2s.
The light emission intensity ratios of individual red light emitting elements and individual blue light emitting elements are not particularly limited, provided that the total red light emission intensity ratio of plural red light emitting elements and the total blue light emission intensity ratio of plural blue light emitting elements are within the above-mentioned range.
Illumination lamp facilities as preferably used for the plant cultivation are equipped with a control part. Emission intensities of red light and blue light, emitted from the irradiation part 11, can be controlled by varying the current value, applied to red light emitting elements 2 and blue light emitting elements 3, by the controlling part.
The plant cultivating illumination lamp 1 as illustrated in FIG. 1 is provided with a pair of electrodes 41 and 42 for red light emitting elements 2 and a pair of electrodes 43 and 44 for blue light emitting elements 3. The plural red light emitting elements 2 are electrically connected by wires (not shown) to the electrodes 41 and 42. The plural blue light emitting elements 3 are electrically connected by wires (not shown) to the electrodes 43 and 44.
The control part equipped in the illumination lamp facility 1 has a function of independently turning on and off the red light emitting elements 2 and the blue light emitting elements 3 by supplying electric current, respectively, via the electrodes 41 and 42 to the red light emitting elements 2 and via electrodes 43 and 44 to the blue light emitting elements 3.
The control part is provided with a lamp controller (i.e., light emission intensity-controlling means), which can turn on and off the red light emitting elements 2 and the blue light emitting elements 3 so that the red light and the blue light are irradiated alternately or concurrently, and for a desired period of time. Thus, the light emission intensity ratio of red light to blue light, irradiated from the light irradiation part 11, can be controlled so as to achieve the desired growth of plants.
The lamp controller used includes, for example, one type which can vary the light emission intensities of the red light emitting elements and the blue light emitting elements 3 by supplying different electric currents to a part or all of the red light emitting elements 2 and/or a part or all of the blue light emitting elements 3, thereby controlling the total emission intensity ratio of red light to blue light, irradiated from the light irradiation part 11; and another type which varies the light emission intensities of the red light emitting elements and the blue light emitting elements 3 by supplying an electric current only to a part of the red light emitting elements 2 and/or a part of the blue light emitting elements 3 to turn on a limited number of light emitting elements, thereby controlling the total emission intensity ratio of red light to blue light, irradiated from the light irradiation part 11.
In the plant cultivation lamp 1, as specifically disclosed in FIG. 1, provided with a light irradiation part 11 having the same number of red light emitting elements and blue light emitting elements, and with a lamp controller, the same electric current can be supplied to all of the red light emitting elements 2 and all of the blue light emitting elements 3, whereby red light and blue light which have the same light emission intensity are irradiated from the light irradiation part 11.
Alternatively, a plant cultivation lamp 1 which is provided with a light irradiation part having the same number of red light emitting elements and blue light emitting elements, but is not provided with a lamp controller can be used in the case when the same electric current is supplied to all of the red light emitting elements 2 and all of the blue light emitting elements 3.
As another operation of the plant cultivation lamp 1, as illustrated in FIG. 1, provided with a light irradiation part having the same number of red light emitting elements and blue light emitting elements, and with a lamp controller, different currents can be supplied to the red light emitting elements 2 and the blue light emitting elements 3 so that the light irradiation part 11 irradiates light with an emission intensity ratio of red light to blue light being 2:1.
Plant Cultivation Method
The method of cultivating a plant according to the present invention will now be explained specifically and more in detail on an embodiment using the illumination lamp 1 as illustrated in FIG. 1.
The plant cultivation method of the present invention comprises a step (A) of irradiating a plant with a red light and a step (B) of irradiating a plant with a blue light, wherein the red light irradiation step (A) and the blue light irradiation step (B) are independently carried out for a predetermined period of time.
By the term “independently” as used herein, we mean that the red light irradiation step (A) and the blue light irradiation step (B) exist separately in the course of plant cultivation.
The procedure of independently carrying out the red light irradiation step (A) and the blue light irradiation step (B) gives a desired plant growth effect, as compared with a plant cultivation procedure wherein red light and blue light are concurrently irradiated. The reason for which the desired enhanced plant growth can be attained by independently carrying out the red light irradiation step (A) and the blue light irradiation step (B), is not clear. But, it is presumed that the photosynthesis process caused by the red light is different from that caused by the blue light due to the fact that chlorophyll exhibits a red light absorption peak distinct from a blue light absorption peak, therefore, when light and blue light are concurrently irradiated, the photosynthesis process by red light and the photosynthesis process by blue light undesirably interfere with each other.
By the term “predetermined period of time” as used herein, we mean an optional length of time within the course of plant cultivation. The maximum length of the predetermined period of time equals to the entire time length of the course of plant cultivation. The minimum length thereof can be voluntarily set provided that the desired plant growth-enhancing effect can be attained. The predetermined period of time can be expressed in unit of hour, day or minute depending upon the particular length of time.
Each of the red light irradiation step (A) and the blue light irradiation step (B) is carried out independently at least once, preferably at least two times, for the predetermined period of time. In the case when the red light irradiation step (A) and the blue light irradiation step (B) are carried out dividedly in two times or more, time length of each operation of the red light irradiation step (A) and time length of each operation of the blue light irradiation step (B) are preferably at least one hour, for example, 1 to 48 hours, and more preferably at least 3 hours, for example, 3 to 24 hours.
The red light irradiation step (A) and the blue light irradiation step (B) can be carried out either alternately and continuously, or intermittently with interposition of an operation of concurrently irradiating plant with red light and blue light between each operation of the red light irradiation step (A) and each operation of the blue light irradiation step (B), or with pause of irradiation between each operation of the red light irradiation step (A) and each operation of the blue light irradiation step (B).
Transfer between operation of red light irradiation step (A) and operation of red light irradiation step (A) can be conducted either instantaneously or over a certain length of time. The transfer may be conducted in stages. During the transfer, operation of red light irradiation step (A) and operation of red light irradiation step (A) may overlap with each other, or a pause of irradiation may intervene between operation of red light irradiation step (A) and operation of red light irradiation step (A).
Alternate flashing of red light irradiation and blue light irradiation by quickly repeating light flashing with a high frequency such as 1 Hz or higher is excluded from the light irradiation procedure adopted for independently carrying out the red light irradiation step (A) and the blue light irradiation step (B) for a predetermined period of time in the plant cultivation method of the present invention.
It is presumed that the mechanism of growth occurring due to light irradiation in the plant cultivation method of the present invention is different from the mechanism of growth occurring due to the alternate flashing of red light irradiation and blue light irradiation by quickly repeating light flashing with a high frequency such as 1 Hz or higher. In other words, in the case when operation of light irradiation is quickly repeated, the plant growth effect brought about by the flashed light irradiation would vary greatly depending upon each time length of light irradiation. In the plant cultivation method of the present invention, each operation for the red light-irradiation step (A) and each operation for the blue light irradiation step (B) are carried out for a period of time sufficient for allowing the photosynthesis reaction and related reactions to occur in connection with environmental changes to an extent such that the desired plant growth is achieved.
In contrast, in the alternate flashing of red light irradiation and blue light irradiation by quickly repeating light flashing with a high frequency such as 1 Hz or higher, the photosynthesis reaction and related reactions do not occur to the desired extent. This is because each flashing of red light irradiation and blue light irradiation occurs for a very short length of time which is insufficient for achieving the plant growth effect. The plant growth effect achieved by the alternate flashing is similar to that achieved by the operation of concurrently irradiating plant with red light and blue light.
The plant cultivation method of the present invention can be adopted for any period of time within the entire course of plant cultivation spanning from immediately after the germination of seeds or immediately after the plantation to the harvest.
The plants to be cultivated in the method of the present invention are not particularly limited, and this term means plants in a broad sense which include leaf plants, water plants, root plants, fruit plants, pluses, grains, seeds, algae, house plants, mosses and vegetable planktons.
The procedure of independently carrying out the red light irradiation step (A) and the blue light irradiation step (B) using the above-mentioned illumination lamp facility gives a desired plant growth effect.
An illumination lamp facility equipped with a control part having a lamp controller (i.e., light emission intensity-controlling means) in the irradiation part having red light emitting elements and blue light emitting elements is preferably used for this procedure. Emission intensities of red light and blue light, emitted from the red light emitting elements and the blue light emitting elements can be desirably controlled for the particular kinds of plants by varying the current value, applied to the red light emitting elements and the blue light emitting elements, by the controlling part.
The illumination lamp facility equipped with a control part in the irradiation part having red light emitting elements and blue light emitting elements is also advantageous as compared with the conventional illumination lamp facility having red light emitting means and blue light emitting means which are separately arranged, because the light emitting means can be easily and steadily arranged in the illumination lamp facility and undesirable deviation of the irradiation directions of red light and blue light can be minimized.
The light irradiation part as illustrated in FIG. 1 has a rectangular shape in a plan view, therefore, the illumination lamp facility can be easily set in the position in which a conventional illumination facility such as straight tube fluorescent lamp is set.
The illumination lamp facility is preferably provided with a converter, built in the lamp, of converting alternating current to direct current for LED because of ease in setting and effective utilization of space. The terminal on one side and the terminal on the other side are preferably utilized separately for the red light emission and blue light emission in view of arrangement of electrical sources built therein and dispersion in generation of heat.
Further the illumination lamp facility is preferably provided with a dimmer for controlling LED in plural lamps and desirably adjusting the light emission intensity ratio.
The plant cultivation method of the present invention should not be construed to be limited to the modes mentioned above.
The illumination lamp facility described above is equipped with a lamp controlling means for controlling light emission intensity. The plant cultivation can also be carried out using an illumination lamp facility not equipped with the lamp controlling means. This illumination lamp facility is advantageous in that a lamp controlling means and its accessories can be omitted and the cost of equipment production is reduced.
EXAMPLES
Preparation of Plants
In the following examples, reference example and comparative example, leaf lettuce (variety: summer serge) was tested for observing the growth state.
Six seeds of leaf lettuce were sown at equal intervals in each test group on a peat van culture medium, and irradiated with fluorescent light for 12 hours of light per day to be thereby germinated. The seeds were placed under the same irradiation conditions over a period of three days spanning from seeding to germination. Thereafter seedlings were raised under irradiation with fluorescent light to give leaf lettuces for test.
Example 1
The raised seedlings of leaf lettuces for test were placed in an environment control room wherein temperature-, humidity- and carbon dioxide concentration-controlling means were provided. The test leaf lettuces were cultivated for 20 days under conditions of: concentration of carbon dioxide in an air atmosphere, 1,000 ppm (except for the concentration in nutritious liquid); atmosphere temperature of 22-25° C.; and humidity of 60% RH, while the leaf lettuces were irradiated with red light and blue light as follows.
An illumination lamp for plant cultivation used had a light emitting part provided with 180 red light emitting elements and 60 blue light emitting elements. Each red light emitting element was comprised of a LED emitting red light having a center wavelength of 660 nm and a wavelength region of 640-680 nm, and each blue light emitting element was comprised of LED emitting blue light having a center wavelength of 450 nm and a wavelength region of 430-470 nm. The illumination lamp further had a control part for controlling the light emitting part so that the red light emitting elements and blue light emitting elements are independently turned on and off.
The red light emitting elements exhibited a total emission intensity, i.e., a total photosynthetic photon flux density (PPFD), of 150 μmol/m2·s. The blue light emitting elements exhibited a total emission intensity, i.e., a total photosynthetic photon flux density (PPFD), of 50 μmol/m2·s. Thus the total emission intensity ratio of red light to blue light was 3:1.
The red light-irradiation step and the blue light irradiation step were alternately and repeatedly carried out for 12 hours for each irradiation time per day, i.e., each light irradiation step was carried out separately and continuously over a period of 12 hours per day. There was no time for which the light irradiation was ceased.
When 20 days elapsed, the light irradiation was stopped and grown leaf lettuces were harvested.
Nutritious liquids used were prepared from tap-water into which carbon dioxide was not intentionally added. The tap-water contained approximately 10 ppm of carbon dioxide. To the tap-water, urea as a nitrogenous fertilizer, superphosphate of lime as a phosphatic fertilizer, and potassium chloride as a potassic fertilizer were added. A thus-prepared aqueous nutritious liquid contained 17 me/L of nitrogen, 4 me/L of phosphorus, 8 me/L of potassium, 4 me/L of calcium, and trace amounts of magnesium and iron. pH of the nutritious liquid was adjusted to 6.
Into the aqueous nutritious liquid, carbon dioxide was incorporated by using a commercially available nanobubble-generator. A thus-prepared carbon dioxide-added nutritious liquid containing 2,000 ppm of carbon dioxide was used at the red light irradiation step (A) and the blue light irradiation step (B).
Ten leaves were collected from the periphery of each rolled lettuce, and average area (cm2) of leaves and fresh weight (g) of above-ground part of the lettuce were measured. A content (mg) of calcium per 100 g of the fresh weight was also measured. The cultivation results are shown in Table 1.
In the examples, reference example and comparative example, the cultivation results are expressed by relative indexes as the values obtained in Example 1 being 100.









TABLE 1









Examples, Comparative Examples


















Ref.
Co.



Ex. 1
Ex. 2
Ex. 3
Ex. 4
Ex. 1
Ex. 1*1

















Red light irradiation








CO2 concentration in
2000
2000
4000
1000
10
2000


liquid fertilizer (ppm)


Blue light irradiation


CO2 concentration in
2000
1000
2000
500
10
2000


liquid fertilizer (ppm)


Cultivation results *2


Fresh weight of above-
102
119
106
103
100
47


ground part


Average area of leaves
98
110
115
102
100
61


Calucium content
112
131
122
120
100
102





Note;


*1In Comparative Example 1, red light and blue light were concurrently irradiated.


*2 Relative indexes as values in Reference Example 1 being 100






Example 2
By the same procedures as described in Example 1, leaf lettuces were cultivated except that two kinds of carbon dioxide-added nutritious liquids were prepared by incorporating carbon dioxide by using a nanobubble-generator, one of which had a carbon dioxide content of 2,000 ppm and the other of which had a carbon dioxide content of 1,000 ppm. The nutritious liquid having a carbon dioxide content of 2,000 ppm was used at the red light irradiation step (A), and the nutritious liquid having a carbon dioxide content of 1,000 ppm was used at the blue light irradiation step (B). All other cultivation conditions remained substantially the same as in Example 1. The cultivation results are shown in Table 1.
Example 3
By the same procedures as described in Example 1, leaf lettuces were cultivated except that two kinds of carbon dioxide-added nutritious liquids were prepared by incorporating carbon dioxide by using a nanobubble-generator, one of which had a carbon dioxide content of 4,000 ppm and the other of which had a carbon dioxide content of 2,000 ppm. The nutritious liquid having a carbon dioxide content of 4,000 ppm was used at the red light irradiation step (A), and the nutritious liquid having a carbon dioxide content of 2,000 ppm was used at the blue light irradiation step (B). All other cultivation conditions remained substantially the same as in Example 1. The cultivation results are shown in Table 1.
Example 3
By the same procedures as described in Example 1, leaf lettuces were cultivated except that two kinds of carbon dioxide-added nutritious liquids were prepared by incorporating carbon dioxide by using a nanobubble-generator, one of which had a carbon dioxide content of 1,000 ppm and the other of which had a carbon dioxide content of 500 ppm. The nutritious liquid having a carbon dioxide content of 1,000 ppm was used at the red light irradiation step (A), and the nutritious liquid having a carbon dioxide content of 500 ppm was used at the blue light irradiation step (B). All other cultivation conditions remained substantially the same as in Example 1. The cultivation results are shown in Table 1.
Reference Example 1
By the same procedures as described in Example 1, leaf lettuces were cultivated except that a nutritious liquid used was prepared without incorporation of carbon dioxide (a nanobubble-generator was not used), and the nutritious liquid used contained 3 ppm of carbon dioxide. The nutritious liquid having a carbon dioxide content of 3 ppm was used at the red light irradiation step (A) and the blue light irradiation step (B). All other cultivation conditions remained substantially the same as in Example 1. The cultivation results are shown in Table 1.
Comparative Example 1
By the same procedures as described in Example 1, leaf lettuces were cultivated except that the red light irradiation step and the red light irradiation step were concurrently and continuously carried out for 12 hours per day; and the lamp was left put out for 12 hours per day. The concurrent irradiation of red light and blue light, and the lamp leaving put out were repeated for 20 days. All other cultivation conditions remained substantially the same as in Example 1. The cultivation results are shown in Table 1.
As seen from Table 1, when at least a nutritious liquid used at the red light irradiation step (A) is a nutritious liquid containing carbon dioxide added therein (Example 1), the content of calcium in lettuces is large as compared with the case when a nutritious liquid containing carbon dioxide added therein is not used (Reference Example 1). When a nutritious liquid is used at each of the red light irradiation step (A) and the blue light irradiation step (B), and the nutritious liquid used at the step (A) contains carbon dioxide at a concentration higher than that in the nutritious liquid used at the step (B) (Examples 2-4), the fresh weight of above-ground part of lettuces and the leave areas of lettuces, and the calcium content in lettuces are large as compared with the case when a nutritious liquid containing carbon dioxide added therein is not used (Reference Example 1).
When red light and blue light are concurrently irradiated (Comparative Example 1), the calcium content in lettuces is small, and the fresh weight of above-ground part of lettuces and the leave areas of lettuces are very poor.

## Claims

1. A method of cultivating a plant comprising a step (A) of irradiating a plant with a red light and a step (B) of irradiating a plant with a blue light,
wherein the red light irradiation step (A) and the blue light irradiation step (B) are independently, alternately and repeatedly carried out for a predetermined period of time under cultivation conditions such that a nutritious liquid containing fertilizer ingredients is applied at each of the red light irradiation step (A) and the blue light irradiation step (B), and the nutritious liquid applied at the red light irradiation step (A) contains carbon dioxide at a concentration higher than the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B).

2. The method of cultivating a plant according to claim 1, wherein the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) is in the range of 1,000 ppm to 5,000 ppm, and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is in the range of 500 ppm to 2,000 ppm.

3. The method of cultivating a plant according to claim 1, wherein the difference between the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is at least 300 ppm.

4. The method of cultivating a plant according to claim 1, wherein the difference between the concentration of carbon dioxide in the nutritious liquid applied at the red light irradiation step (A) and the concentration of carbon dioxide in the nutritious liquid applied at the blue light irradiation step (B) is in the range of 500 ppm to 1,000 ppm.

5. The method of cultivating a plant according to claim 1, wherein, among the nutritious liquids applied at the red light irradiation step (A) and the blue light irradiation step (B), at least the nutritious liquid applied at the red light irradiation step (A) contains carbon dioxide added therein by bubbling carbon dioxide, or by incorporating therein carbonated water containing carbon dioxide added by bubbling.

6. The method of cultivating a plant according to claim 1, wherein, among the nutritious liquids applied at the red light irradiation step (A) and the blue light irradiation step (B), at least the nutritious liquid applied at the red light irradiation step (A) contains nanobubbles of carbon dioxide which are prepared by bubbling carbon dioxide therein.

7. The method of cultivating a plant according to claim 1, wherein the nutritious liquids applied at the red light irradiation step (A) and at the blue light irradiation step (B) contain nitrogen, phosphorus and potassium as fertilizer ingredients, and, a content of at least one of the fertilizer ingredients in the nutritious liquid applied at the red light irradiation step (A) is larger than the content of the corresponding fertilizer ingredient in the nutritious liquid applied at the blue light irradiation step (B).

8. The method of cultivating a plant according to claim 1, wherein the plant is selected from algae, phytoplankton and euglena.

9. The method of cultivating a plant according to claim 1, wherein the red light-irradiation step (A) and the blue light irradiation step (B) are alternately and repeatedly carried out over a period of at least one hour for each irradiation time.

10. The method of cultivating a plant according to claim 1, wherein the red light irradiation step (A) and the blue light irradiation step (B) are carried out using an illumination lamp facility having red light emitting elements and blue light emitting elements, both of which are capable of being independently operated, and the red light emitting elements and the blue light emitting elements exhibit a light emission intensity ratio of red light to blue light of at least 1:1 as expressed by a ratio of photosynthetic photon flux density of red light to blue light.

11. The method of cultivating a plant according to claim 1, wherein the red light irradiation step (A) and the blue light irradiation step (B) are carried out using an illumination lamp facility having red light emitting elements and blue light emitting elements, both of which are capable of being independently operated, and the red light emitting elements and the blue light emitting elements exhibit a light emission intensity ratio of red light to blue light in the range of 2:1 to 9:1 as expressed by a ratio of photosynthetic photon flux density of red light to blue light.

12. The method of cultivating a plant according to claim 1, wherein time length of each operation of the red light irradiation step (A) and time length of each operation of the blue light irradiation step (B) are 1 to 48 hours.