EXPERT STATEMENT 

Study Title: 

ADSORPTION COEFFICIENT OF DEA/Acid 

anhydride reaction product 

Data Requirement I Test Guidelines: 

OECD Guideline No. 106 

OECD Guideline No. 121 

EC Directive 2001/59 C.19 

Study Director: 

Mrs. Alessandra Tognucci 

Study Completion Date: 

February 05, 2004 

Test Facility: 

RCC Ltd 

Environmental Chemistry & Pharmanalytics 

CH-4452 ltingen/Switzerland 

Sponsor: 

Rockwool International A/S 

Hovedgaden 584 

DK-2640 Hedehusene 

Denmark 

RCC Study Number: 

848639 

Page 1 of 10 
RCC Study Number 848639 

DENAcid anhydride reaction product 

Title: 

Sponsor: 

Monitoring Scientist: 

Expert Statement by: 

STATEMENT 

EXPERT STATEMENT 

Adsorption Coefficient of DEA/Acid 

anhydride reaction product 

Rockwool International A/S 

Hovedgaden 584 

DK-2640 Hedehusene 

Denmark 

Mr. Flemming A. Simonsen 

Danish Toxicology Centre 

Kogle Aile 2 

DK-2970 H0RSHOLM 

Denmark 

Mrs. Alessandra Tognucci 

RCC Ltd 

Environmental Chemistry & Pharmanalytics 

CH-4452 lti ngen/Switzerland 

Page2 

Under Study 848639 RCC was supposed to estimate the adsorption coefficient of DEAl Acid 

anhydride reaction product on soil using high performance liquid chromatography (HPLC) 

according to OECD Guideline No. 121. 

The adsorption constant determination by HPLC has to be performed under specific 

conditions with a cyanopropyl column as stationary phase and with one of the following 

solvent mixtures as mobile phase: 

• methanol/water (55:45°/o v/v) 

• methanol/0.01 M citrate buffer pH 6.0 (55:45 v/v) 

Other organic solvent mixtures like ethanol/water or acetonitrile/water may be tried if 

methanol/water is not appropriate. 

DEAl Acid anhydride reaction product is a complex mixture of different monomers, dimers, 

trimers and polymers. This mixture cannot be analysed with one single chromatographic 

technique. Several techniques like size exclusion chromatography, ion chromatography, 

derivatization followed by HPLC analysis and conventional reversed phase HPLC were used 

for the analysis of the mixture. Different detectors had to be used for the detection of the 

components: refractive index detector, conductivity detector and UV detector. Mobile phases 

contained a large volume fraction of water (often acidified) to achieve retention of the 

monomers. 

Due to the special properties and the different analytical methods required for the analysis of 

the test item mixture, the measurement of the adsorption coefficient by HPLC according to 

the OECD Guideline 121 was not applicable to DEA/Acid anhydride reaction product. 
RCC Study Number 848639 

DEA/Acid anhydride reaction product 

References 

EXPERT STATEMENT Page 10 

[1] W.J. Lyman, W.F. Reehl, D.H. Rosenblatt: Handbook of Chemical Property 

Estimation Methods, American Chemical Society, 1990. 

[2] Estimation Program Interface (EPI) Suite Version 3.11, PCKOCWIN TM estimation 

programU.S. Environmental Protection Agency, June 10, 2003. 

[3] McCall, P. J., D.A. Laskowski. R.L. and H.J. Dishburger, 1981. 

Measurement of sorption constants of organic chemicals and their use in 

environmental fate analysis. p. 89-109. Testprotocols for environmental fate and 

movement of toxicants. Assoc. of Off. Anal. Chern., Arlington, VA. 

[4] Y.-Y. Hsieh et al., J. Chromatogr. A, 952 {2002), 255-266. 

[5] Handbook of Chemistry and Physics, 81st ed., 2000-2001. 

[6] D.O. Perrin, B. Dempsey and E.P. Serjeant, pKa prediction for organic acids and 

bases, Clap man and Hall (1981 ). 

RCC Environmental Chemistry & Pharmanalytics 

Mrs. Alessandra Tognucci Stu,..." JI:Mr-zn"'ltr*-

Dr. Jorn Schreitmuller 

Manager of Analytical Chemistry