# EXPERTSTATEMENT
StudyTitle: ADSORPTION COEFFICIENTOF DEA/Acid 

### anhydride reaction product
Data RequirementITestGuidelines: OECD Guideline No. 106 OECD Guideline No. 121 EC Directive2001/59 C.19 StudyDirector: Mrs.AlessandraTognucci StudyCompletion Date: February05, 2004 Test Facility: RCC Ltd Environmental Chemistry& Pharmanalytics CH-4452 ltingen/Switzerland Sponsor: Rockwool InternationalA/S Hovedgaden 584 DK-2640 Hedehusene Denmark RCC StudyNumber: 848639 Page 1of 10RCC Study Number848639 DENAcidanhydride reaction product
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Monitoring Scientist:

Expert Statement by:


# STATEMENT EXPERT STATEMENT
Adsorption Coefficientof DEA/Acid anhydride reaction product Rockwool InternationalA/S Hovedgaden 584 DK-2640 Hedehusene Denmark Mr. Flemming A. Simonsen Danish ToxicologyCentre Kogle Aile 2 DK-2970 H0RSHOLM Denmark Mrs. Alessandra Tognucci RCC Ltd Environmental Chemistry & Pharmanalytics CH-4452 ltingen/Switzerland Page2

Under Study 848639 RCC was supposed to estimate the adsorption coefficient of DEAl Acid anhydride reaction product on soil using high performance liquid chromatography (HPLC) according to OECD Guideline No. 121. The adsorption constant determination by HPLC has to be performed under specific conditions with a cyanopropyl column as stationary phase and with one of the following solvent mixtures as mobile phase:
• methanol/water (55:45°/o v/v) • methanol/0.01 M citrate buffer pH 6.0 (55:45v/v) Other organic solvent mixtures like ethanol/water or acetonitrile/water may be tried if methanol/water is not appropriate. DEAl Acid anhydride reaction product is a complex mixture of different monomers, dimers, trimers and polymers. This mixture cannot be analysed with one single chromatographic technique. Several techniques like size exclusion chromatography, ion chromatography, derivatization followed by HPLC analysis and conventional reversed phase HPLC were used for the analysis of the mixture. Different detectors had to be used for the detection of the components: refractive index detector, conductivitydetectorand UV detector. Mobile phases contained a large volume fraction of water (often acidified) to achieve retention of the monomers. Due to the special properties and the different analytical methods required forthe analysis of the test item mixture, the measurement of the adsorption coefficient by HPLC according to the OECD Guideline 121 was notapplicableto DEA/Acid anhydride reaction product.RCC Study Number848639 DEA/Acid anhydride reaction product 

### References EXPERTSTATEMENT Page 10



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### 1981. Measurement of sorption constants of organic chemicals and their use in environmental fate analysis. p. 

### 89-109. Testprotocols for environmental fate and movement oftoxicants. Assoc. ofOff. Anal. Chern., Arlington, VA. [4] Y.-Y. Hsieh et al., J. Chromatogr. A, 

### 952 {2002), 255-266. [5] Handbookof Chemistryand Physics, 

### 81st ed., 

### 2000-2001. [6] D.O. Perrin, B. Dempsey and E.P. Serjeant, pKa prediction for organic acids and bases, Clapman and Hall 

### (1981).

RCC Environmental Chemistry& Pharmanalytics 

### Mrs. Alessandra Tognucci 

### Stu,..." JI:Mr-zn"'ltr*- 

### Dr. Jorn Schreitmuller ManagerofAnalytical Chemistry