## UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

## WASHINGTON; D.C . 20460

December 17 , 2014 MEMORANDUM SUBJECT : 

## EPA File Symboi62719-EUP-AA (MON 87411 Combined Trait Insect Control for Corn). Section G Calculations Decision N2: 491621 DP N2: 422031 OFFICE OF CHEMICAL SAFETY AND POL LU TION PREVENTION



## FROM : 

## Kathleen Mart i n, Chemist , M.S.E.S. 

# {J /(aflif\ 

# 12 /11/ 111 Microbial Pro duc ts Branch , Biopesticides and Po llut ion Prevent io n Div is ion THROUGH : 

## Alan Reynolds, Team Leader Microbial Products Branch Biopesticides and Pollut ion Preventio TO: 

## Jeannine Kausch, Regulatory Action Leade r Microbial Prod ucts Branch Biopesticides and Pollut io n Prevention Division Dow AgroSciences , LLC is requesting an experimental use permit (EUP) to test a new plant-incorporated protectant (PIP) technology, MON 87 411, combined trait insect control corn , combinations necessary for their production, and controls. In summary, Dow would like to test 17 PIPs in 16 localit ies (15 states plus the Commonwealth of Puerto Rico) using a max imum of 9,038 acres (6,361 of which is expected to be PIP acreage). The attached Data Evaluat ion Record provides a review of the Section G calculations.

# Data Evaluation Record Reviewed by: 

### Secondary Review: Kathleen Martin , Chemist .• ( 

# I A l an Reynolds , M . S . 

# A( I 

# LJ \ l / 

# lj_ STUDY TYPE: 

### Sect i on G calculations for 62719-EUP-AA TEST GU I DELINE: 

### Not app l icable MRID: 

### None DP BARCODE: 422031 TEST MATERIAL: 

### Combinations of PIP events that are made up of one or more of the following individual events: MON 89034 , MON 88017 , TC1507 , DAS-59122-7 , and MON 87411. SPONSOR: 

### Dow AgroSciences , LLC TESTING FACILITY: 

### NA TITLE OF REPORT: 

### Administrative Materials in Support of an Application for an Experimental Use Permit to Test Stacked Trait Insect Control Corn , Combinations Necessary for the Production and Controls Containing the Events , MON 89034 , MON 88017 , MON 87411, TC1507 and DAS-59122-7 (EPA File Symbol 62719-EUP - X AUTHORS: 

## G . Bradfisch and 

## L. Baker SUBMISSION DATE: 

### May 27 , 2014 CONCLUSION: 

### D i screpancies between EPA and Dow ' s calculations were resolved. The data and calculated values prov i ded in th i s Data Eva l uat i on Record ( DER) are correct. CLASSIFICATION: 

### Acceptable GOOD LAB PRACTICE: 

### Not app li cable 

## BACKGROUND Dow AgroSciences, LLC is requesting an experimental use permit (EUP) to test a new plant-incorporated protectant (PIP) techno lo gy, MON 87 411 , combined trait insect control corn, combinations necessary for their product i on , and controls. They intend to test 17 PIP events in 16 localities (15 states plus the Commonwealth of Puerto Rico) a using max im um of 9,038 acres . Prov ided in Table 1 are the 17 events along with their proteins. Notice that two events-MON 88017 and MON 87411-share a common trait (i.e., Cry3Bb1). I n calculating the values presented i n 

## Table 5 

## and Tab le 6 , the Cry3Bb 1 values are a sum of the contribution from MON 88017 and MON 87 411 . Table 2 provides an overall summary of the maxim um number of acres , by locali ty , expected to be planted under this EUP. About 70 percent of the total acreage will be planted with PIP seed. Dow is planning to carry out this EUP using two Proto cols . Protocol 1 (P1 ) -breeding , enables inbred and hybrid development along with observation nurseries while Protoco l 2 (P2) -charac terization - involves testing and collection of product characterization data including : phenotypic and agronomic observations; product efficacy and yield benefits ; characterization , performance ; and regulatory data . Table 3 shows the acreage distribution between the two protocols ; P1 will use 47 percent of the PIP acres and P2 , 53 percent. Table 4 is the last of the data tables (the rest of the tables show calculated values based on the data provided by Dow). It shows the acres (total: P1 +P2) broken down by PIP and state. To see this information for the P1 and P2 acres, refer to Appendix I.Dow: 62719-EUP - AA December 2014 DP422031 

## CALCULATIONS Assumptions: provided by Dow (confidential business information);


## Amount of PIP-Containing Seed per Protein: 

## The amount of PIP-containing seed for each PIP protein was calculated using the following formu la; the values are shown in Table 5 : lb d 

### •th t . 

## X 4 7 lb seed #ACRES with protein 

## x see w1 pro em = 

# x ACRE _ 

## _j where protein X= 

### Cry lA . lOS, Cry2Ab2, Cry3Bb (from MON 88017), Dv49dsRNA, Cry3Bbl (from MON 87411), CrylF, Cry34Abl, or Cr y3 5 Abl 

## Note that there are two sources for Cry38b1 (MO N 88017 and ). In calculating the pounds per PIP protein , the Cry38b1 values ref ount from both sources . The acres used in the calculation were total acres (P1 + P2 ); to see these values for P1 and P2 acres, refer to Appendix II . Amount of PIP Material: 

## The amount of PIP protein (in lbs) was calculated as follows; see Tab l e 6 for the values:

. 47 lb seed ACRES . 

# I lb protem= 

## x -- x %protem ACRE

**~------ --------**  

## Again , the Cry3Bb1 value for represents the contribution from MON 88017 and and the acres are total acres (P1 +P2) ; the amount of protein for t he P1 and P2 is provided in Appendix Ill.
BPPD COMMENTS: In adding up and arraying the acres, EPA and Dow agreed ; however , for the data tables where calculations were involved (i.e., Dow Table 7 : Proposed Total PIP Test Material Pounds of Seed Quantity by State ; and Table 8 : Proposed Tota l PIP Test Material Active Ingredient Quantities by State), we disagreed . After communicating with Dow we were able to resolve the differences .
DER -2T a ble 1 PIP E ve n ts a nd Traits Ev e nt: Trait(s)

: R e gistered PIP s:

E P A R e . N<:? MON 89034

: [C ry 1A.1 05, Cry2Ab2

]

52 4 -575, 52 4 -597 M ON 88017

: [C ry 38b 1] TC150 7: [Cry1 F] DAS-59122-7

: [Cry34Ab1

, Cry35 Ab1]

52 4 -551 68467-2 2996 4 -4

, 2996 4 - 10 , 68467

- 5 2996 4 - 5 , 29964

- 6 , 68467

- 6 524-576

, 52 4 - 606 Dow: 6 2 719
- EUP-AA De c ember 2014 DP422031 TC1507

x DAS-5912 2- 7 : [Cry1 F ] , [Cry34Ab1

, Cry35 Ab1] MON 890 34 X MON 880 1 7 : [Cry
1 A. 1 05 , Cry2Ab2]

, (Cry38b1] MON 89034
X TC150 7: [Cry
1 A.105

, Cry2Ab2]

, [Cry1F]

524 - 585
, 524-612

, 68467

- 12

, 68467

- 21 MON 89034 X MON 88017 X TC1507

: [Cry1A.105, Cry2Ab2]

, [Cry3Bb1],

[ Cry 1 F ] MON 8903 4 X MON 88017 X DAS-59122-7

: [Cry1A.105, Cry2Ab2]

, [Cry3Bb1

] , [Cry34Ab1

' Cry35 Ab 1 ] MON 8 9 034 X TC1507

X DAS
- 59122-7

: [Cry1A.105, Cry2Ab2

] , [Cry 1 F ] , [Cry34Ab

1 ' C ry 35 Ab 1] 524-587

, 68 4 67 - 8 52 4 - 589

, 68 4 67 - 10 524 - 588, 68 4 67 - 9 MON 89034
X MON 88017
X TC1507

X DAS
- 59122-7

: I [Cry
1 A .105

, C ry 2 Ab 2 ], [Cry3 B b1 ] ,[C ry 1 F ] , [Cry34Ab1

, Cry35Ab

1 ] 5 24 - 5 8 1 ' 52 4 - 595

, 68 4 6 7 - 7 , 68 46 7- 16 Unr egi s tered PIPs: MON 8741 1: lr ovSnf7 dsR N A, Cry38b 1) MON 87411
x TC1507

: I [DvSnf7 dsRNA, CrY3Bb1)

, [ Cry1 F ) M O N 874 1 1 x DAS-59122-7

: I [DvSnf7 dsRNA

, Cry3Bb1)

, [Cry34Ab1

, Cry35 Ab1) M O N 890 3 4 x M O N 87411
x TC1507

: [Cry1A

. 105
, Cry2Ab2)

, [Dvsnf7

, Cry38b1)

, [Cry1F] MON 874 11 x TC150 7 x DAS
- 5912 2- 7 : I [D DvSnf7 dsRNA

, Cry3Bb1)

, [Cry1F)

, [Cry3 4 Ab1

, Cry35 Ab1 ) MON 8903 4 x M ON 87411
x TC150 7 x DAS-5912

2 -7 : I [Cry1A.105

, Cry2Ab2]

, [ OvSnf7 dsRNA

, Cry3Bb1]

, [Cry1F

] , [Cry34Ab1

, Cry35 Ab1)

D ER-3

## Table 2

I 

## AR

H 

### l l I N 

### lA 

## KS M N MO 

## NE NO OH 

### PA 

## PR 

# s o T

X 

# w

I 

### Tota l % 

### of Total Acres 

# s f th E U P A e um m a ry o c r eage : non 

## PI P 8 d I 

## or e r , an N on PIP B o rder Regist ered 

## P I P ( P1+P 2) Un Re g istered T o

t a l PI P 

## 1 0 2 24 12 36 58 59 329 172 501 98 178 330 206 536 1 08 198 335 263 598 103 188 335 268 603 18 18 38 22 60 98 178 328 211 539 1 8 18 33 19 52 83 118 332 212 5 4 4 68 93 2 6 9 1 4 3 41 2 6 8 93 269 14 3 41 2 10 2 24 12 36 80 100 385 210 595 78 108 307 196 503 80 1 4 5 

# I 262 169 4 31 83 118 307 196 503 1 , 061 1,616 3 , 907 2,454 6,361 ( 1 1 . 7) (17.9) (43. 2 ) (27 . 2) (70.4) 

## d PIP TO TA L A cr es



## 4 8 6 1 8 812 904 894 96 815 88 745 573 573 4 8 775 689 656 704 9,038 ( 1 00) Not e: P1= P ro t ocol 1 (breedmg and a ssoc1ate d da t a) ; P2 = Protocol2 (chara cte nzatlon) 

## T a ble 3 

## A ere a g e IStn U IOn e tw ee n 

# E t I P 

## t x penm e n a r o o co s Dow: 

### 62719 - EUP - AA December 2014 DP422031



## P 1 P2 TOTAL (P1+ P 2) AR HI I L 

### IN lA 

### KS M N 

### MO 

### N

E N D OH 

### PA P

R SD TX W

I 

### Total: % P I P Acres: R egistered UnRegistered 

### Total P1 Registered UnRegistered 

### Total P2 Registered



### 0 0 0 24 12 36 24 12 4 60 184 205 112 317 329 1 6 5 90 255 165 116 28 1 330 165 90 255 170 173 3 4 3 335 165 90 255 170 178 3 48 335 0 0 0 38 22 60 38 165 90 255 163 121 284 328 0 0 0 33 19 52 33 165 90 255 167 122 289 332 165 90 255 104 53 157 269 165 90 255 104 53 157 269 0 0 0 24 12 36 24 1 6 5 90 255 2 20 120 340 385 165 90 255 142 106 2 4 8 307 1 65 90 255 9 7 79 176 2 62 16 5 90 255 14 2 106 

### 248 307 1 , 939 1 , 050 2 , 989 1 , 968 1, 404 3 , 372 3 , 907 ( 30 . 5 ) (1 6 . 5 ) ( 47 . 0 ) ( 30 . 9 ) ( 22 . 1) ( 53 . 0 ) ( 61.4 ) No t e: P1= Protoco l1 (breed1n g and assoc1ated data) , P2=Protocol2 ( characte n zallo n) UnRegistered 

### TotaiP1+P2 12 36 172 501 206 536 263 5 9 8 268 603 22 60 211 539 19 52 212 544 

### 143 412 143 412 12 36 210 595 196 503 169 431 196 503 2,454 6 , 361 (38 . 6) (100)



### DE R - 4TabARHILINlAKSMNMONENOOHPAPRsoTXwTota'...-REGISTEREDPIPS:";-'d".....N...,c;N......co.....coco0zz...,00uC§::E:::E1-2 22 22626 26262723263526 23263526 2326352 2 24272326332 2 2 5282327352323 23 25232323252 22235 35 3535272326302521242527232630328297322382__I----------···-----..........u)(..........";-.....Nco0coNz...,z;;;00"?::E::Een)()()(<(Cl..................,...,...,X000.....a>a>a>cococozzu001-::E::E:::E2 2 4 227 27593132 32312932 32 34 3232 32343244 4 4323231293 3 4 3323231292424312424 2431242 2 4 235 35 35 35292931262424272129293126363 363422349·-UNREGISTEREDPIPS:.....)(";-;::;N..........;;;0N0...,;;;...,...,a>uend;"?C§1-en<(;::;)(<()()(•Cl..........Cl.....)(N)('d"c;..........;;;...............co0d;co0coco...,z...,zzuOenC§0u001-:iF<(::E1-::E::E)(xClXX)()(X,.._)(.....;:!;"...J.....;;N...J...,<(...,...........b0.....o"'...............a>.....cococococococozzzu1-zzzzz•0ot-m0000oen::ee§:::E::!!"en::E::E::E::E::Een2 2 2242 2 2 2 2 2122727 2732926 263927272717232313233033253331 315320632313233540266535326526332313233545266535 32652684 4 4384244 44223231323283825333131532113 3 3335 23 3 3 3 1932313233239253331315321224242426924232424 242414324242426924232424 242414322224 2 2 2 2 221235353538535 35 35 35 35 3521029 28 2930735253028 28501962423 2426230232523 23451692928 293073525302828501963633553633',907417315447.3633575552.454Notes:P1=Protocol1(breedingandassociateddata);P2=Protocol2(characterization);ForabreakoutoftheP1andP2acres,seeAPPENDIX1a.0:::a.....II0:::z001-z361050158536985981086031036018539985218544834126841268361059580503784318050383.6,3611,061Dow:62719-EUP·AADecember2014DP422031...II00::1-·wa0z:0::0IllC)248596181788121989041888941896178815188311874593573935732481007751086891456561187041,6169,038.DER-5

## Tab I

I

I AR H 

### I L IN lA 

### KS MN MO NE 

### NO O

H PA PR 

## so T X 

## w Total 

## e5 Cry1A.105 940 13 , 066 14 , 006 14 , 993 14 , 993 1,598 14 , 006 1,269 14 , 053 10 , 434 10,434

940 1 4,805 13 , 019 11 , 092 13,019 162 , 667 

## Amount of Seed (lbs) Expected to Be Used for Each Prote i n Pounds of P I P-Contain i ng Seed Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35Ab1 Cry3b1 940 564 1 , 034 752 752 1 , 034 13,066 8 , 084 14,288 10 , 669 10,669 14 , 570 14,006 9 , 682 1 5 , 087 13 , 113 13,113 16 , 638 14,993 12 , 361 16 , 215 15 , 228 15 , 228 19 , 458 14,993 12 , 596 16 , 215 15 , 228 15 , 228 19 , 693 1 , 598 1 , 034 1,692 1,504 1,504 1 , 880 14,006 9 , 917 15 , 087 13 , 019 13,019 16 , 873 1 , 269 893 1,363 1,222 1 , 222 1 , 551 14 , 053 9 , 964 15 , 134 13 , 113 13 , 113 16 , 920 10 , 434 6 , 72 1 11 , 515 9,071 9 , 071 12,314 10 , 434 6,721 11 , 515 9 , 071 9 , 071 1 2,314

940 

### 564 1,034 752 752 1 , 034 14 , 805 9 , 870 16,450 13 , 160 13 , 160 8 , 095 13 , 0 1 9 9 , 212 14,100 11,891 11,89 1 15 , 604 11 , 092 7,943 1 2,079 10,011 10,011 13 , 301 13 , 019 9 , 212 14,100 11 , 891 11,891 15 , 604 162,667 115 , 338 176 , 908 149 , 695 149 , 695 196,883



### .. Note: These values represent the amounts of P I P-contammg based on Total (P1+P2) acres; please Refer to Appendix II for the P1 and P2 breakout. Dow : 6 2719 - EUP-AA D ecember 2014 DP422031



## DER-6Dow: 62719-EUP-AA December 2014 DP422031 

## T ab l e 6 Amount of P I P P rotein (lbs, Q ) 

## Expected to B e Us e d

AR l bs 

### 9 HI lbs 

### 9 IL lbs 

### 9 IN lbs 

### 9 l A l bs 

### 9 KS lbs 

### 9 MN lbs 

### 9 MO lbs 

### 9 N E lbs 

### 9 NO lbs 

### 9 OH lb s 

### 9 PA lbs 

### 9 P R lbs 

### 9 ' 

# so lbs 

### 9 ' TX lbs 

### 9 : WI lbs g TOT l b s 

### 9 : 

## Pounds of Protein C ry1 A. 10 5 Cry2 Ab 2 D v Snf7 C ry1 F C ry34Ab1 C ry 35 A b 1 Cry3 b1 dsRNA 0.006580 0.001974 9 . 588E-09 0.007652 0 . 07069 0 . 001730 0.01344

2.985 0 . 8954 4.3 4 9E-06 3 . 471 32.06 0 .7 845 6 . 097 0 . 09146 0 . 02744 1 .374E-07 0 . 1057 1 .0 03 0 . 02454 0 . 1872

4 1.49 12 . 45 6 . 234E-05 4 7 . 96 454.9 11 . 13 84.89 0 . 09804 0 . 02941 1.646E-07 0 . 1116 1 . 233 0.03016 0 . 2063

44.47 13.34 7.466E-05 50.64 559 . 1 13 .68 93.57 0. 1 050 0 . 03149 2 . 101E-07 0 . 1200 1.43 1 0 . 03502 0.2241 4 7 . 60 14 . 28 9.532 E- 05 54 . 43 649 . 3 15 . 89 101.7 0.1050 0 . 03149 2 . 141 E-07 0 . 1200 1.431 0 . 03502 0.2254 47 . 60 14 .28 9 . 713E-05 54.43 649 . 3 15.89 102.2 0.01119 0 . 003356 1.758E-08 0 . 01252 0.1414 0.003459 0 . 02430

5 . 074 1 . 522 7.973E-06 5 . 679 64 .1 3 1 . 569 11 . 02 0 . 09804 0 . 029 41 1.686E-07 0.1116 1.224 0.02994 0 . 2076

44.47 13 . 34 7 . 647E-05 50 . 64 555.1 13 . 58 94 . 15 0.008883 0.002665 1.518E-08 0 . 01009 0 . 1149 0 . 002811 0.01939

4.029 1 . 209 6 . 886 E -06 4 . 575 52 . 10 1.275 8 . 794 0.09837 0.02951 1 . 694E-07 0 .1 120 1 . 233 0.030 1 6 0 . 2078

44.62 13 . 39 7 . 683E-05 50 . 80 559 . 1 13 . 68 94 . 27 0 . 07304 0.02191 1 . 143E-07 0 . 08521 0 . 8527 0 . 02086 0.1600

33 . 13 9 . 939 5 . 183E-05 38 . 65 386.8 9.463 72 . 58 0 . 07304 0 . 02191 1.143E-07 0 . 08521 0 . 8527 0 . 02086 0 . 1600

33 . 13 9 . 939 5 . 183 E- 05 38 . 65 386 . 8 9 . 463 72 . 58 0 . 006580 0 . 001974 9.588E-09 0 . 007652 0 . 07069 0.001730 0 . 01344

2.985 0 . 8954 4 . 349E-06 3 . 471 32 . 06 0.7845 6.097 0.1036 0.03109 1 .678E-07 0 . 1217 1 . 237 0 . 03027 0 . 2352 47 . 01 1 4 .1 0 7 . 611E-05 55 . 22 561 . 1 13 . 73 106 .7 0 . 09113 0.02734 1 . 566E-07 0.1043 1.1 1 8 0 . 02735 0 .191 3 41 . 34 12 . 40 7. 1 03 E -05 47 . 33 507 . 0 12 . 41 86 . 77 0 . 07764 0 . 02329 1 . 350E-07 0 . 08938 0.9410 0.02303 0 . 1616

35 . 22 10 . 5 7 6 . 125E-05 4 0 . 54 426.8 10 . 44 73 . 28 0.09113 0 . 02734 1.566E-07 0 . 1043 1 . 118 0 . 02735 0.1913

41 . 34 12.40 7.103E-05 47 . 33 507 . 0 12.41 86.77 1 . 1 39 0 . 3416 1.961 E-06 1 . 309 14.07 0 .3 443 2 . 428 516 . 5 154.9 8.89 4 E-0 4 593 . 8 6 , 383 156 . 2 1 '101



### . . Note : These values represent the amounts of P I P-contam1ng base d on Total (P1 +P2) acres ; please Refer to Appendix Ill for the P1 and P2 breakout.



## DER-7APPENDIX I.A

- - --- REG I STERED PIPS :
AR HI I L IN lA KS MN MO NE NO OH PA PR so TX w Tota .... "'

C) a> co z 0 :::;; 0 8 15 15 15 0 15 0 15 15 15 0 15 15 15 15 173 ,_ c; co ,_ co **~**

z 0 u

::;: 1- 0 0 8 8 15 1 5 15 1 5 15 15 0 0 15 1 5 0 0 15 15 15 15 15 15 0 0 15 15 15 15 15 15 15 1 5 173 173 Prot '

-- - - 11 A

, ";- N N "';- N a; 'L? (/) c( 0
N X o;

,._ :h C) U') u <( 0

1- 0 0 8 8

1 5 1 5 15 15 15 1 5 0 0

1 5 15 0 0 15 15 15 15 15 15 0 0 15 15 15 1 5 15 1 5 15 15 173 173 - -- bv PIP and Stat ";- N
,_ N C) ch U') a>
u <( 'L?

0
X

X (/) <(

,_

,_ ,_ 0 r-,_

c; c; c; X ON

co ,_ co co ,_ CON

co

C) co co **~** co-

z U') z z zen

0

u 0 0 u o'L?

:::;;

1- :::;; :::;; 1- **:::;;~**

)(

)( )( )( X xo

....

.... .... .... ;s; '<I" X

(") (") M M <">,_

C) C) C) C) **~**

a> a> a> o> ,._ **~~**

co co co co ' co
N z<:>

z z z ZN z

0

0 0 o- 0 01-

:::;;

:::;; :::;;

**:::;;~** :::;; :::i! X

0

0 0 0 0 0

8 4 0 1 2 8 8 8

1 5 15 15 15 15 15

15 15 15 15 15 15

1 5 15 15 1 5 15 15

0 0 0 0 0 0

15 15 15 15 15 15

0 0 0 0 0 0

15 15 15 15 15 15 15 15 15 15 15 15

15 15 15 15 15 15

0 0 0 0 0 0

15 15 15 15 15 15

1 5 15 15 15 15 15

1 5 15 15 15 15 15

1 5 15 15 15 15 15

173 205 177 1 73 1 7 3 173

**L-~ ~~**  **---L~~~~~~--~~~~~ ~-=**  **~~**

N ote : P1= Proto co l 1 (breed i ng and associated data); P2 = Protocol2 (ch aracterizat ion) UNREQISTERED PIPS: ";- X
,_

N ,_
C)

N C)
U') U')
u o; - 'L? u
1- 1-

X (/) X

**~**

<( **~**

0

N

- X ....

....

,_ o; ,_ ,_ ,_

C)

U') co C) co

U')

ch z U') z

u **~** 0 u 0

1-

:::;; 1- :::;;

X

X X X X ,._ a.

-'

- - - .... - ..,. . -' a:

<( ; ; ; (") - MN g a. 0::

**~**

.... a"' a: w

b ,_ ..... ,._ ,_ **"'~** -' 5 ...J

co

co co co co **co~ ~**  **~** g

Iii 1-

z z z z z Z' 0

0 0 0 0 0 0(/) co

::>

::> 1- z co 1-

(/) :::;; :::;; :::;; :::;; :::;; **:::;;~** (/)

0

0 0 0 0 0 0 0 0 0 0 0

124 8 8 20 8 8 8 60 184 30 15 229

165 15 15 15 15 15 15 90 255 4 0 50 3 4 5

165 15 15 15 1 5 1 5 15 90 255 40 50 3 4 5

165 15 15 15 1 5 15 15 90 255 4 0 50 3 4 5

0 0 0 0 0 0 0 0 0 0 0 0

165 1 5 15 15 15 1 5 15 90 255 4 0 50 3 4 5 i

0 0 0 0 0 0 0 0 0 0 0 0 I

165 15 15 15 15 15 15 90 255 40 50 3 4 5

165 15 15 15 15 15 15 90 255 4 0 50 3 4 5

165 15 15 15 15 15 15 90 255 4 0 50 345 I

0 0 0 0 0 0 0 0 0 0 0 0

165 15 15 15 15 15 15 90 255 40 50 345

165 15 15 15 15 15 1 5 90 255 40 50 345

1 65 1 5 15 15 15 15 1 5 90 255 40 50 3 45

165 15 1 5 15 15 15 15 90 255 40 50 345

1 , 939 1 73 173 185 173 173 173 1050 2989 470 565 4,0 2 4 DER - 8APPENDIX 1.8

- -- REGISTERED PIPS :
AR H l l IN lA KS MN MO NE NO OH PA PR so TX w I I Tota v M C) "' co z 0 :::;; 2 18 12 11 11 2 12 2 13 8 8 2 20 12 10 12 155 ,..._ c; .., ,._ co C)
z .,.,
0

u

:::;;t2 2 18 18 8 11 8 11 8 1 1 2 2 8 11 2 2 8 12 8 8 8 8 2 2 20 20 8 11 6 9 8 1 1

1 24 149 - -- - - - . - -- - - - -- -- - -- --- Prot' 12A bv PIP and Sta t' "' ";-

N N
,._ c;; N
C) .,., "' "'
u ch OL;> £3 (f)

)(

<

,._ ,._ )( 0 ,._ ,._

**~**

c; ,._ )( **~** 0

";- C) 0 oN

..,

,._ .., ,._ """"

**~**

.., :i'l .., .., C) .., **~** "'

a; z z <0 .,., **~~**

0 u 0 z u

OL;> 0

:::;; t- :::;; t- :::;;<

(f) )( )( )( ::;: )( xO

**~** < X

0 v v 'd' **~ ~**  **~** ..

N <") M M M M ,._

a; )( a: a: C) 0 C) oo

...... "' "' a> a>.,.,

.,.,

0 co .., .., cO co **co~**

ch "' z z z z z zu

< u 0 0 0 0 0 ot-

0

t- :::;; :::;; :::;; ::;:,_ :::;; :::;; )( 2 2 2 4 2 2 2 2

18 19 19 19 19 19 19 19

20 17 17 16 14 17 16 17

20 17 17 19 17 17 16 17

20 17 17 19 17 17 16 17

4 4 4 4 4 4 4 4

1 8 17 17 1 6 14 1 7 16 17 5 3 3 4 3 3 3 3

20 17 17 16 14 17 16 17

10 9 9 16 9 9 9 9

10 9 9 16 9 9 9 9 2 2 2 4 2 2 2 2

20 20 20 20 20 20 20 20

15 14 14 16 11 14 13 14

10 9 9 12 6 9 8 9

15 14 14 16 11 14 13 14

209 1 90 190 217 17 2 190 182 190

**L_ ~~~~~~**  **~~~~~~ ~~~~**  **~~~~ ~~~~** 

Note: P 1- Protocol1 (breeding and assoc i ated data); P2 =Protocol2 (cha ra cterization) UNREGISTERED PIPS : ";- )(
,._

N ,._
C)

N C)
.,., **~** .,.,
u

"' u

.,., t- ch t-

";-

)( < )(

N

**~** 0 **~**

N a;

"¢ )( ....

,._ ,._ ,._ ,_

C)

.,., .., C) ..,

.,.,

ch z .,., z ..-1

u 0 u 0

< **~**

t- 0 :::;; t- :::;;

)(

X X )( 0

)( ,._ a.

..-1

**~** ... ... 'o:t ... v' ..-1 a: 0::: 1-

g

**~ ~**  **~** M **~** """" **~** a.

C) a"' ..-1 a: w 0

,._ ...... ,._ m ...... **a>~** 0

cO cO cO co <0 **<0~** g **~** z

t- z z z z z Z' z 0::: **~**

CD CD

:::> 0 0 0 0 0 0(/)

:::> 0 0 0

(f)

:::;; :::;; :::;; :::;; :::;; :::;:;3

(f) I- z en (!)

24 2 2 2 2 2 2 12 10 2 36 48

205 18 18 19 19 1 9 19 1 1 2 28 44 317 389

165 18 10 18 16 16 38 116 58 128 281 467

170 25 11 50 20 17 50 173 68 148 343 559

170 30 11 50 20 17 50 178 63 138 348 549

38 4 2 4 4 4 4 22 18 18 60 96

1 63 23 10 18 16 16 38 121 58 128 284 470

33 5 2 3 3 3 3 19 18 18 52 88

167 24 10 18 16 16 38 122 43 68 289 400

104 9 8 9 9 9 9 53 28 43 157 228

104 9 8 9 9 9 9 53 28 43 157 228

24 2 2 2 2 2 2 12 10 2 36 48

220 20 20 20 20 20 20 120 40 50 340 430

142 20 10 15 13 13 35 106 38 58 248 3 44

97 15 8 10 8 8 30 79 40 95 176 31 1

142 20 10 15 13 13 35 106 43 68 248 359

1,968 244 142 262 190 184 382 1404 591 1051 3372 5,014

------- --

----' D ER-9

## APPENDIX 

## II.A

A

R H

I I L IN 

### lA KS MN MO 

## N

E NO OH P A 

### P

R 

## so T

X WI 

### Total Cry1A.105 0 5,076 6,345 6 ,3 45 6 , 345

0 6 , 345

0 6 , 3 4 5 6,345 6,345

0 6 , 345 6 , 345 6 , 345 6,345 74,871 

## Amount of Seed ( l bs) E xpected to Be Used for Each Protein for Protocol 1 Acres Pounds of P I P-Contain i ng Seed Cry2Ab2 

### Dvsnf7 Cry1F Cry34Ab1 Cry35Ab1 0 0 0 0 0 5 , 076 . 2.820 5,452 3,572 3,572 6 , 345 4,230 7 , 050 5 , 640 5,640 6 , 345 4 ,230 7 , 050 5 , 640 5 , 640 6 , 345 4 , 230 7 , 050 5 , 640 5 , 640

0 0 0 0 0 6 , 345 4 , 230 7 , 050 5,640 5 , 640

0 0 0 0 0 6 . 345 4 ,2 30 7 , 050 5,640 5,640 6 , 345 4,230 7,050 5,640 5,640 6 , 345 4 ,230 7,050 5,640 5,640

0 0 0 0 0 6 , 345 4 , 230 7 , 050 5,6 4 0 5 , 640 6 , 345 4 , 230 7 , 050 5 , 640 5 , 640 6,345 4 , 230 7,050 5 , 640 5 , 640 6,345 4,230 7,050 5 , 640 5,640 74 , 871 49 ,3 50 83,002 65,612 65,612 

### N ote : P1= Protocol1 (breed1ng and associated data); P2=Protocol2 (charactenzatlon) 

## A P P E ND I X 11 . 8

AR HI IL IN 

### lA KS MN MO 

## N

E N

O O H PA 

### P

R 

## so TX WI 

### Total Cry1A.105 940 7 , 990 7,661 8,648 8,648 1 , 598 7 , 661 1 , 269 7,708 4 , 089 4 , 089 940 8 , 460 6 , 674 4 , 7 4 7 6 , 674 87,796 

## Amount of Seed (lbs) E xpected to Be Used for Each Protein for P rotocol 

## 2 Acres Pounds of P IP -Contain i ng Seed Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35Ab1 940 564 1,034 752 752 7,990 5,264 8 , 836 . 7,097 7 , 097 7,661 5,452 8 , 037 7,473 7 , 473 8,648 8,131 9 , 165 9 , 588 9 , 588 8 , 648 8,366 9 , 165 9 , 588 9 , 588 1 , 598 1 , 034 1 , 692 1 , 504 1 , 504 7 , 661 5 , 687 8 , 037 7 , 379 7 , 379 1 , 269 893 1 , 363 1,222 1,222 7,708 5 , 734 8 , 084 7,473 7,473 4,089 2 , 491 4 , 465 3 , 431 3,431 4.089 2 , 491 4,465 3,431 3 , 431 940 564 1 ,0 34 752 752 8 , 460 5 , 640 9,400 7 , 520 7,5 20 6 , 674 4 , 982 7 , 050 6 , 251 6 . 25 1 4 , 747 3 , 713 5 , 029 4 , 371 4 , 371 6 , 674 4 , 982 7,050 6 , 251 6 , 251 87 , 796 65 , 988 93 , 906 84 , 083 84,083



# - Note: P1- Protocol1 (breedmg and assoc1ated data) , P2-Protocol2 (cha ractenzatlon) 

### Cry3b1 0 4 , 888 7 , 755 7 , 755 7,755

0 7,755

0 7,755 7,755 7 , 755

0 7 , 755 7 , 755 7 , 755 7 ,7 55 90 ,193



### Cry3b1

1 , 034 9 , 682

**~.883** 1 1,703 11 , 938 1 , 880 9 , 118 1 , 551 9,165 4,559 4 , 559 1 , 034 10 , 340 7,8 49 5 , 546 7 , 849 106 , 690 

## DER-10

## APPENDIX 

## II Acres 

## I. 

# A Amount of PIP Protein (lbs, g) Expected to Be Used for Protocol1 Pounds of Protein Cry1A.105 Cry2Ab2 Dvsnf7 Cry1F Cry34Ab 1 Cry35 Ab1 Cry3b1 

## AR l bs : 

### 0 0 0 0 0 0 0 g : 

### 0 0 0 0 0 0 0 

## HI lbs : 

### 0 . 03553 0 . 01066 4 . 794E-08 0 . 04034 0 . 3358 0 . 008216 0 . 06101 g : 

### 16 . 12 4.835 2 . 175E-05 18 . 30 152 . 3 3 . 727 27 . 67 

## IL lbs : 

### 0 . 04442 0 . 01332 7.191 E-08 0 . 05217 0 . 5302 0 . 01297 0.1008 g : 

### 20 . 15 6 . 044 3.262E-05 23 . 66 240 . 5 5 . 884 45 . 73 

## IN lbs : 

### 0 . 04442 0.01332 7 . 191 E-08 0 . 05217 0 . 5302 0 . 01297 0 . 1008 g : 

### 20 . 15 6 . 044 3.262E -0 5 23.66 240 . 5 5 . 884 45.73 lA lbs: 

### 0 . 04442 0.01332 7.191 E-08 0.05217 0 . 5302 0.01297 0.1008 g : 

### 20.15 6 . 044 3.262E-05 23.66 240 . 5 5 . 884 45 . 73 KS 

### lbs : 

### 0 0 0 0 0 0 0 g : 

### 0 0 0 0 0 0 0 

## MN lbs : 

### 0 . 04442 0.01332 7 . 191E-08 0.05217 0 . 5302 0 . 01297 0 . 1008 g : 

### 20 . 15 6 . 044 3.262E-05 23 . 66 240 . 5 5 . 884 45.73 MO lbs : 

### 0 0 0 0 0 0 0 g : 

### 0 0 0 0 0 0 0 

## NE l bs : 

### 0 . 044 42 0 . 01332 7.191 E-08 0 . 05217 0 . 5302 0 . 01297 0 . 1008 g : 

### 20 . 15 6 . 044 3 . 262E-05 23 . 66 240 . 5 5 . 884 45 . 73 

## NO lbs : 

### 0 . 04442 0 . 01332 7 . 191 E-08 0 . 05217 0 . 5302 0 . 01297 0 . 1008 g : 

### 20 . 15 6 . 044 3 . 262E-05 23 . 66 240 . 5 5 . 884 45 . 73 

## OH lbs : 

### 0 . 04442 0 . 01332 7 . 191E-08 0 . 05217 0 . 5302 0 . 01297 0.1008 g : 

### 20 . 15 6 . 044 3 . 262E-05 23 . 66 240 . 5 5 . 884 45 . 73 

## PA lb s : 

### 0 0 0 0 0 0 0 g : 

### 0 0 0 0 0 0 0 

## PR lbs : 

### 0 . 04442 0 . 01332 7 . 191 E-08 0 . 05217 0.5302 0 . 01297 0 . 1008 g : 

### 20 . 15 6 . 044 3.262E-05 23 . 66 240 . 5 5.884 45.73 

# so lbs : 

### 0 . 04442 0.01332 7 .1 91E-08 0.05217 0.5302 0 . 01297 0.1008 g : 

### 20 . 15 6 . 044 3 . 262E-05 23 . 66 240 . 5 5 . 884 45.73 

## TX lbs : 

### 0 . 04442 0.01332 7.191E-08 0.05217 0 . 5302 0.01297 0.1008 g : 

### 20 . 15 6.044 3.262E-05 23 . 66 240 . 5 5 . 884 45 . 73 

## WI lbs : 

### 0 . 04442 0 . 01332 7.191 E-08 0 . 05217 0.5302 0 . 01297 0.1008 g : 

### 20 . 15 6 . 044 3 . 262E-05 23 . 66 240 . 5 5 . 884 45.73 

## TOT lbs : 

### 0 . 5241 0 . 1572 8.390E-07 0.6142 6 . 168 0 . 1509 1 . 170 g : 

### 237 . 7 71 . 32 3 . 805E-04 278 . 6 2 , 798 68 . 45 530.7 Note : P1- Protocol1 ( breedmg and assoctated data ); P2-Protoco l 2 ( charactenzallon )



### DER- 11

## APPENDIX 

## II 

## 1.8 Amount of PIP Protei n (lbs , g) Expected to Be Used for P ro toco l1 Acres Pounds of Protein Cry1A . 105 Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35 Ab1 Cry3b1 

### AR lbs : 

### 0 . 006580 0 . 001974 9 . 588E-09 0 . 007652 0 . 07069 0 . 001730 0 . 01344 g : 

### 2 . 985 0 . 8954 4.349E-06 3 . 471 32 . 06 0 . 7845 6 . 097 

## HI lbs : 

### 0 . 05593 0 . 01678 8.949E-08 0 . 06539 0 . 6671 0 . 01632 0 .1 261 g : 

### 25 . 37 7.611 4 .0 59E-05 29 . 66 302 . 6 7 . 404 57 . 22 

## IL lbs : 

### 0 . 05363 0 . 01609 9.268E-08 0.05947 0 .7 025 0.01719 0 . 1055 g : 

### 24 . 32 7 . 297 4 . 204E-05 26 . 98 318 . 6 7 .7 96 47 . 84 

## IN lbs : 

### 0 . 06054 0.01816 1 . 382E-07 0.06782 0 . 9013 0 . 02205 0 . 1233 g : 

### 27 . 46 8.238 6.270E-05 30.76 408 . 8 10 . 00 55.93 lA lbs : 

### 0 . 06054 0 . 01816 1 . 422E-07 0.06782 0 . 9013 0 . 02205 0.1246 g : 

### 27.46 8 . 238 6 . 451 E-05 30 . 76 408 . 8 10.00 56.52 KS lbs : 

### 0 . 01119 0.003356 1 . 758E-08 0.01252 0.1414 0.003459 0 . 02430 g: 

### 5 . 074 1 . 522 7.973E-06 5 . 679 64 . 13 1 . 569 11 . 02 

## MN lbs : 

### 0 . 05363 0 . 01609 9.668 E-0 8 0 . 05947 0 . 6936 0 . 01697 0.1068 g: 

### 24 . 32 7 . 297 4.385E-05 26 .98 314 . 6 7 . 698 48.43 

## MO lbs : 

### 0 . 008883 0 . 002665 1 .518 E-08 0 . 01009 0 . 1149 0 . 002811 0.01939 g : 

### 4 . 029 1 . 209 6.886E - 06 4 . 575 52 . 10 1 . 275 8 . 794 

## NE lbs : 

### 0 . 05396 0 . 01619 9.748E - 08 0 . 05982 0 .7 025 0 . 01719 0 .1 070 g : 

### 24.47 7.342 4.422E-05 27 . 13 318 . 6 7 . 796 48 . 54 

## NO lbs : 

### 0 . 02862 0 . 008587 4 . 235E-08 0 . 03304 0.3225 0 . 007891 0 . 05920 g : 

### 12.98 3 . 895 1 . 921E-05 14 . 99 146 . 3 3 . 579 26 . 85 

## OH lbs : 

### 0 . 02862 0 . 008587 4 .235E-08 0 . 03304 0 . 3225 0 . 007891 0 . 05920 g : 

### 12 . 98 3 . 895 1 . 921E-05 14.99 146 . 3 3.579 26.85 PA lbs : 

### 0 . 006580 0 . 001974 9 . 588E-09 0 . 007652 0.07069 0 . 001730 0.01344 g : 

### 2 . 985 0 . 8954 4.349E-06 3.471 32 . 06 0 . 7845 6 . 097 PR lbs : 

### 0 . 05922 0.01777 9.588E-08 0.06956 0 . 7069 0 . 01730 0 . 1344 g : 

### 26 . 86 8 . 059 4.349E-05 31 . 55 320.6 7.845 60 . 97 

# so lbs : 

### 0 . 04672 0 . 01402 8.469E-08 0 . 05217 0 . 5876 0 . 01 4 38 0 . 09048 g : 

### 21 . 19 6 . 357 3 . 842E-05 23.66 266.5 6.521 41 . 04 

## TX lbs : 

### 0 . 03323 0 . 009969 6 . 312E-08 0 . 03721 0.4109 0 . 01005 0 . 06075 g : 

### 15 . 07 4 . 522 2.863E-05 16 . 88 186 . 4 4 . 560 27 . 55 

## WI lbs : 

### 0 . 04672 0.01402 8 . 469 E-08 0 . 052 1 7 0 . 5876 0 . 01438 0 . 09048 g : 

### 21 . 19 6 . 357 3 . 842E-05 23.66 266 . 5 6 . 521 41.04 

## TOT lbs : 

### 0 . 6146 0.1844 1 . 122E-06 0 . 6949 7 . 904 0 . 1934 1 . 258 g : 

### 278 . 8 83.63 5 . 088E-04 315.2 3 , 585 87 .7 2 570 . 8 Note: P1- Protoco l 1 (breedrng and assoc rated data ) , P2 - Protocol2 ( charactenzatron }



### DER- 12