UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

WASHINGTON; D.C. 20460 

December 17, 2014 

MEMORANDUM 

SUBJECT: EPA File Symboi62719-EUP-AA (MON 87411 Combined 

Trait Insect Control for Corn). Section G Calculations 

Decision N2: 491621 

DP N2: 422031 

OFFICE OF CHEMICAL SAFETY 

AND POLLUTION PREVENTION 

FROM: Kathleen Martin, Chemist, M.S.E.S. {J /(aflif\ 12/11/ 111 Microbial Products Branch , 

Biopesticides and Pollution Prevention Division 

THROUGH: Alan Reynolds, Team Leader 

Microbial Products Branch 

Biopesticides and Pollution Preventio 

TO: Jeannine Kausch, Regulatory Action Leader 

Microbial Products Branch 

Biopesticides and Pollution Prevention Division 

Dow AgroSciences, LLC is requesting an experimental use permit (EUP) to test a new 

plant-incorporated protectant (PIP) technology, MON 87 411, combined trait insect control corn, 

combinations necessary for their production, and controls. In summary, Dow would like to test 

17 PIPs in 16 localities (15 states plus the Commonwealth of Puerto Rico) using a maximum of 

9,038 acres (6,361 of which is expected to be PIP acreage). The attached Data Evaluation Record 

provides a review of the Section G calculations. 
Data Evaluation Record 

Reviewed by: 

Secondary Review: 

Kathleen Martin, Chemist .• ( I 

Alan Reynolds, M.S. A( I LJ \ l / lj_ 

STUDY TYPE: Section G calculations for 62719-EUP-AA 

TEST GUIDELINE: Not applicable 

MRID: None 

DP BARCODE: 422031 

TEST MATERIAL: Combinations of PIP events that are made up of one or more of the following individual 

events: MON 89034, MON 88017, TC1507, DAS-59122-7, and MON 87411. 

SPONSOR: Dow AgroSciences, LLC 

TESTING FACILITY: NA 

TITLE OF REPORT: Administrative Materials in Support of an Application for an Experimental Use Permit to 

Test Stacked Trait Insect Control Corn, Combinations Necessary for the Production and 

Controls Containing the Events, MON 89034, MON 88017, MON 87411, TC1507 and 

DAS-59122-7 (EPA File Symbol 62719-EUP-X 

AUTHORS: G. Bradfisch and L. Baker 

SUBMISSION DATE: May 27, 2014 

CONCLUSION: Discrepancies between EPA and Dow's calculations were resolved. The data and 

calculated values provided in this Data Evaluation Record (DER) are correct. 

CLASSIFICATION: Acceptable 

GOOD LAB PRACTICE: Not applicable 

BACKGROUND 

Dow AgroSciences, LLC is requesting an experimental use permit (EUP) to test a new 

plant-incorporated protectant (PIP) technology, MON 87 411 , combined trait insect control corn, 

combinations necessary for their production, and controls. They intend to test 17 PIP events in 16 

localities (15 states plus the Commonwealth of Puerto Rico) a using maximum of 9,038 acres. 

Provided in Table 1 are the 17 events along with their proteins. Notice that two events-MON 88017 

and MON 87411-share a common trait (i.e., Cry3Bb1). In calculating the values presented in Table 5 

and Table 6, the Cry3Bb 1 values are a sum of the contribution from MON 88017 and MON 87 411 . 

Table 2 provides an overall summary of the maximum number of acres, by locality, expected to be 

planted under this EUP. About 70 percent of the total acreage will be planted with PIP seed. 

Dow is planning to carry out this EUP using two Protocols. Protocol 1 (P1 )-breeding, 

enables inbred and hybrid development along with observation nurseries while 

Protocol 2 (P2)-characterization- involves testing and collection of product characterization data 

including: phenotypic and agronomic observations; product efficacy and yield benefits; characterization, 

performance; and regulatory data. Table 3 shows the acreage distribution between the two protocols; P1 

will use 47 percent of the PIP acres and P2, 53 percent. 

Table 4 is the last of the data tables (the rest of the tables show calculated values based on the 

data provided by Dow). It shows the acres (total: P1 +P2) broken down by PIP and state. To see this 

information for the P1 and P2 acres, refer to Appendix I. 
Dow: 62719-EUP-AA 

December 2014 

DP422031 

CALCULATIONS 

Assumptions: 

provided by Dow (confidential business information); 

Amount of PIP-Containing Seed per Protein: 

The amount of PIP-containing seed for each PIP protein was calculated using the 

following formula; the values are shown in Table 5: 

lb d •th t . X 4 7 lb seed #ACRES with protein x see w1 pro em = x 

ACRE _ _j 

where protein X= Cry lA.lOS, Cry2Ab2, Cry3Bb (from MON 88017), 

Dv49dsRNA, Cry3Bbl (from MON 87411), CrylF, Cry34Abl, or Cry35 Abl 

Note that there are two sources for Cry38b1 (MON 88017 and ). In 

calculating the pounds per PIP protein, the Cry38b1 values ref ount from both 

sources. The acres used in the calculation were total acres (P1 +P2); to see these 

values for P1 and P2 acres, refer to Appendix II. 

Amount of PIP Material: 

The amount of PIP protein (in lbs) was calculated as follows; see Table 6 for the values: 

. 47 lb seed ACRES . I lb protem= x --x %protem ACRE 

~--------------

Again, the Cry3Bb1 value for represents the contribution from MON 88017 and 

and the acres are total acres (P1 +P2); the amount of protein for the 

P1 and P2 is provided in Appendix Ill. 

BPPD COMMENTS: 

In adding up and arraying the acres, EPA and Dow agreed; however, for the data tables where 

calculations were involved (i.e., Dow Table 7: Proposed Total PIP Test Material Pounds of Seed 

Quantity by State; and Table 8: Proposed Total PIP Test Material Active Ingredient Quantities by 

State), we disagreed. After communicating with Dow we were able to resolve the differences. 

DER-2 
s and Traits 

Event: Trait(s): 

Registered PIPs: EPA Re . N<:? 

MON 89034: [Cry1A.1 05, Cry2Ab2] 524-575, 524-597 

MON 88017: [Cry38b1] 

TC1507: [Cry1 F] 

DAS-59122-7: [Cry34Ab1 , Cry35 Ab1] 

524-551 

68467-2 

29964-4,29964-10, 68467-5 

29964-5, 29964-6, 68467-6 

524-576, 524-606 

Dow: 62719-EUP-AA 

December 2014 

DP422031 

TC1507 x DAS-59122-7: [Cry1 F], [Cry34Ab1 , Cry35 Ab1] 

ON 89034 X MON 88017: [Cry1A.105, Cry2Ab2], (Cry38b1] 

MON 89034 X TC1507: [Cry1A.105, Cry2Ab2], [Cry1F] 524-585, 524-612, 68467-12, 68467-21 

 MON 88017 X TC1507: [Cry1A.105, Cry2Ab2], [Cry3Bb1], [Cry1F] 

 88017 X DAS-59122-7: [Cry1A.105, Cry2Ab2], [Cry3Bb1 ], [Cry34Ab1 ' Cry35 Ab1] 

TC1507 X DAS-59122-7: [Cry1A.105, Cry2Ab2], [Cry1 F], [Cry34Ab1' Cry35 Ab1] 

524-587, 68467-8 

524-589, 68467-10 

524-588, 68467-9 

TC1507 X DAS-59122-7: I[Cry1A.105, Cry2Ab2], [Cry3Bb1 ],[Cry1 F], [Cry34Ab1 , Cry35Ab1] 524-581' 524-595,68467-7, 68467-16 

Unregistered PIPs: 

MON 87411: lrovSnf7 dsRNA, Cry38b1) 

MON 87411 x TC1507: I[DvSnf7 dsRNA, CrY3Bb1), [Cry1 F) 

 87411 x DAS-59122-7: I[DvSnf7 dsRNA, Cry3Bb1), [Cry34Ab1 , Cry35 Ab1) 

 MON 87411 x TC1507: [Cry1A.105, Cry2Ab2), [Dvsnf7, Cry38b1), [Cry1F] 

TC1507 x DAS-59122-7: I[D DvSnf7 dsRNA, Cry3Bb1), [Cry1F), [Cry34Ab1 , Cry35 Ab1 ) 

TC1507 x DAS-59122-7: I[Cry1A.105, Cry2Ab2], [OvSnf7 dsRNA, Cry3Bb1], [Cry1F], [Cry34Ab1 , Cry35 Ab1) 

DER-3 
Table 2 

I 

AR 

H 

ll 

IN 

lA 

KS 

MN 

MO 

NE 

NO 

OH 

PA 

PR 

so 

TX 

w I 

Total 

% of Total Acres 

s ummary o f th e EUP A creage: non PIP I 8 or d er, an 

Non PIP Border 

Registered 

PIP (P1+P2) 

UnRegistered Total PIP 

10 2 24 12 36 

58 59 329 172 501 

98 178 330 206 536 

108 198 335 263 598 

103 188 335 268 603 

18 18 38 22 60 

98 178 328 211 539 

18 18 33 19 52 

83 118 332 212 544 

68 93 269 143 41 2 

68 93 269 143 41 2 

10 2 24 12 36 

80 100 385 210 595 

78 108 307 196 503 

80 145 I 262 169 431 

83 118 307 196 503 

1,061 1,616 3,907 2,454 6,361 

(11. 7) (17.9) (43.2) (27.2) (70.4) 

d PIP 

TOTAL 

Acres 

48 

618 

812 

904 

894 

96 

815 

88 

745 

573 

573 

48 

775 

689 

656 

704 

9,038 

(100) 

Note: P1= Protocol 1 (breedmg and assoc1ated data); P2=Protocol2 (charactenzatlon) 

Table 3 A ere age IStn U IOn etween E xpenmen a t I P ro ot co s 

Dow: 62719-EUP-AA 

December 2014 

DP422031 

P1 P2 TOTAL (P1+P2) 

AR 

HI 

IL 

IN 

lA 

KS 

MN 

MO 

NE 

ND 

OH 

PA 

PR 

SD 

TX 

WI 

Total: 

%PIP Acres: 

Registered UnRegistered Total P1 Registered UnRegistered Total P2 Registered 

0 0 0 24 12 36 24 

124 60 184 205 112 317 329 

165 90 255 165 116 281 330 

165 90 255 170 173 343 335 

165 90 255 170 178 348 335 

0 0 0 38 22 60 38 

165 90 255 163 121 284 328 

0 0 0 33 19 52 33 

165 90 255 167 122 289 332 

165 90 255 104 53 157 269 

165 90 255 104 53 157 269 

0 0 0 24 12 36 24 

165 90 255 220 120 340 385 

165 90 255 142 106 248 307 

165 90 255 97 79 176 262 

165 90 255 142 106 248 307 

1,939 1,050 2,989 1,968 1,404 3,372 3,907 

(30.5) (1 6.5) (47.0) (30.9) (22.1) (53.0) (61.4) 

Note: P1= Protocol1 (breed1ng and assoc1ated data), P2=Protocol2 (charactenzallon) 

UnRegistered TotaiP1+P2 

12 36 

172 501 

206 536 

263 598 

268 603 

22 60 

211 539 

19 52 

212 544 

143 412 

143 412 

12 36 

210 595 

196 503 

169 431 

196 503 

2,454 6,361 

(38.6) (100) 

DER- 4 
- ... . . 

..... 

u 

)( 

..... ..... 

";-

..... N co 0 co N z ..., z ;;; 0 0 "? ::E ::E en )( )( )( <( 

Cl ..... ..... ..... ..., ..., ..., X 0 0 0 

..... a> a> a> co co co 

z z u 0 0 

1- ::E ::E :::E 

2 2 4 2 

27 27 59 31 

32 32 31 29 32 32 34 32 32 32 34 32 

4 4 4 4 

32 32 31 29 

3 3 4 3 

32 32 31 29 

24 24 31 24 

24 24 31 24 

2 2 4 2 

35 35 35 35 

29 29 31 26 

24 24 27 21 

29 29 31 26 

363 363 422 349 

·-

UNREGISTERED PIPS: 

..... 

)( ";-

N ..... ;::; ..... ;;; 0 N 0 ..., ;;; ..., ..., a> en u d; "? C§ 1- en <( ;::; )( <( )( )( • Cl .......... Cl 

..... )( N )( 'd" c; ..... ..... ;;; ..... ..... ..... co 0 d; co 0 co co ..., z ..., z z u Oen C§ 0 u 0 0 1- :iF<( ::E 1- ::E ::E )( xCl X X )( )( X ,.._ )( 

..... ;:!;" ..., ...J ..... ;;N ...J <( ..., ...... 

..... b 0 ..... o"' ..... ..... ..... a> ..... co co co co co co co 

z z zu 1- z z z z z• 0 ot- m 0 0 0 0 oen 

:::E ::!!" en ::E ::E ::E ::E ::E ::ee§ en 

2 2 2 24 2 2 2 2 2 2 12 

27 27 27 329 26 26 39 27 27 27 172 

32 31 32 330 33 25 33 31 31 53 206 32 31 32 335 40 26 65 35 32 65 263 32 31 32 335 45 26 65 35 32 65 268 

4 4 4 38 4 2 4 4 4 4 22 

32 31 32 328 38 25 33 31 31 53 211 

3 3 3 33 5 2 3 3 3 3 19 

32 31 32 332 39 25 33 31 31 53 212 

24 24 24 269 24 23 24 24 24 24 143 

24 24 24 269 24 23 24 24 24 24 143 

2 2 2 24 2 2 2 2 2 2 12 

35 35 35 385 35 35 35 35 35 35 210 

29 28 29 307 35 25 30 28 28 50 196 24 23 24 262 30 23 25 23 23 45 169 29 28 29 307 35 25 30 28 28 50 196 

363 355 363 3',907 417 315 447 . 363 357 555 2.454 

and associated data); P2=Protocol 2 (characterization); For a breakout of the P1 and P2 acres, see APPENDIX 1 

a. 

0::: a. 

....II 0::: 

z 

0 0 

1- z 

36 10 

501 58 

536 98 

598 108 603 103 

60 18 

539 98 

52 18 

544 83 

412 68 412 68 

36 10 

595 80 503 78 

431 80 

503 83 

. 6,361 1,061 

Dow: 62719-EUP·AA 

December 2014 

DP422031 

...II 

0 

0:: 1-· 

w a 

0 z : 0:: 

0 

Ill C) 

2 48 

59 618 

178 812 

198 904 188 894 

18 96 

178 815 

18 83 

118 745 

93 573 93 573 

2 48 

100 775 108 689 145 656 118 704 

1,616 9,038 . 

DER- 5 
Tab I 

I 

I 

AR 

H 

IL 

IN 

lA 

KS 

MN 

MO 

NE 

NO 

OH 

PA 

PR 

so 

TX 

w 

Total 

e5 

Cry1A.105 

940 

13,066 

14,006 

14,993 

14,993 

1,598 

14,006 

1,269 

14,053 

10,434 

10,434 

940 

14,805 

13,019 

11 ,092 

13,019 

162,667 

Amount of Seed (lbs) Expected to Be Used for Each Protein 

Pounds of PIP-Containing Seed 

Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35Ab1 Cry3b1 

940 564 1,034 752 752 1,034 

13,066 8,084 14,288 10,669 10,669 14,570 

14,006 9,682 15,087 13,113 13,113 16,638 

14,993 12,361 16,215 15,228 15,228 19,458 

14,993 12,596 16,215 15,228 15,228 19,693 

1,598 1,034 1,692 1,504 1,504 1,880 

14,006 9,917 15,087 13,019 13,019 16,873 

1,269 893 1,363 1,222 1,222 1,551 

14,053 9,964 15,134 13,113 13,113 16,920 

10,434 6,721 11 ,515 9,071 9,071 12,314 

10,434 6,721 11 ,515 9,071 9,071 12,314 

940 564 1,034 752 752 1,034 

14,805 9,870 16,450 13,160 13,160 8,095 

13,019 9,212 14,100 11,891 11,891 15,604 

11 ,092 7,943 12,079 10,011 10,011 13,301 

13,019 9,212 14,100 11 ,891 11,891 15,604 

162,667 115,338 176,908 149,695 149,695 196,883 .. Note: These values represent the amounts of PIP-contammg based on Total (P1+P2) acres; please 

Refer to Appendix II for the P1 and P2 breakout. 

Dow: 62719-EUP-AA 

December 2014 

DP422031 

DER-6 
Dow: 62719-EUP-AA 

December 2014 

DP422031 

Table 6 Amount of PIP Protein (lbs, Q) Expected to Be Used 

AR lbs 

9 

HI lbs 

9 

IL lbs 

9 

IN lbs 

9 

lA lbs 

9 

KS lbs 

9 

MN lbs 

9 

MO lbs 

9 

NE lbs 

9 

NO lbs 

9 

OH lbs 

9 

PA lbs 

9 

PR lbs 

9' 

so lbs 

9' 

TX lbs 

9: 

WI lbs 

g 

TOT lbs 

9: 

Pounds of Protein 

Cry1A.105 Cry2Ab2 DvSnf7 Cry1F Cry34Ab1 Cry35 Ab1 Cry3b1 dsRNA 

0.006580 0.001974 9.588E-09 0.007652 0.07069 0.001730 0.01344 

2.985 0.8954 4.349E-06 3.471 32.06 0.7845 6.097 

0.09146 0.02744 1.374E-07 0.1057 1.003 0.02454 0.1872 

41.49 12.45 6.234E-05 47.96 454.9 11 .13 84.89 

0.09804 0.02941 1.646E-07 0.1116 1.233 0.03016 0.2063 

44.47 13.34 7.466E-05 50.64 559.1 13.68 93.57 

0.1050 0.03149 2.101E-07 0.1200 1.431 0.03502 0.2241 

47.60 14.28 9.532E-05 54.43 649.3 15.89 101.7 

0.1050 0.03149 2.141 E-07 0.1200 1.431 0.03502 0.2254 

47.60 14.28 9.713E-05 54.43 649.3 15.89 102.2 

0.01119 0.003356 1.758E-08 0.01252 0.1414 0.003459 0.02430 

5.074 1.522 7.973E-06 5.679 64.1 3 1.569 11.02 

0.09804 0.02941 1.686E-07 0.1116 1.224 0.02994 0.2076 

44.47 13.34 7.647E-05 50.64 555.1 13.58 94.15 

0.008883 0.002665 1.518E-08 0.01009 0.1149 0.002811 0.01939 

4.029 1.209 6.886E-06 4.575 52.10 1.275 8.794 

0.09837 0.02951 1.694E-07 0.1 120 1.233 0.03016 0.2078 

44.62 13.39 7.683E-05 50.80 559.1 13.68 94.27 

0.07304 0.02191 1.143E-07 0.08521 0.8527 0.02086 0.1600 

33.13 9.939 5.183E-05 38.65 386.8 9.463 72.58 

0.07304 0.02191 1.143E-07 0.08521 0.8527 0.02086 0.1600 

33.13 9.939 5.183E-05 38.65 386.8 9.463 72.58 

0.006580 0.001974 9.588E-09 0.007652 0.07069 0.001730 0.01344 

2.985 0.8954 4.349E-06 3.471 32.06 0.7845 6.097 

0.1036 0.03109 1.678E-07 0.1217 1.237 0.03027 0.2352 

47.01 14.1 0 7.611E-05 55.22 561 .1 13.73 106.7 

0.09113 0.02734 1.566E-07 0.1043 1.1 18 0.02735 0.191 3 

41 .34 12.40 7.103E-05 47.33 507.0 12.41 86.77 

0.07764 0.02329 1.350E-07 0.08938 0.9410 0.02303 0.1616 

35.22 10.57 6.125E-05 40.54 426.8 10.44 73.28 

0.09113 0.02734 1.566E-07 0.1043 1.118 0.02735 0.1913 

41 .34 12.40 7.103E-05 47.33 507.0 12.41 86.77 

1.139 0.3416 1.961 E-06 1.309 14.07 0.3443 2.428 

516.5 154.9 8.894E-04 593.8 6,383 156.2 1 '101 . . Note: These values represent the amounts of PIP-contam1ng based on Total (P1 +P2) acres; please 

Refer to Appendix Ill for the P1 and P2 breakout. 

DER-7 
APPENDIX - - --- I.A 

REGISTERED PIPS: 

AR 

HI 

IL 

IN 

lA 

KS 

MN 

MO 

NE 

NO 

OH 

PA 

PR 

so 

TX 

w 

Tota 

.... 

"' C) a> 

co 

z 

0 

:::;; 

0 

8 

15 

15 

15 

0 

15 

0 

15 

15 

15 

0 

15 

15 

15 

15 

173 

,_ 

c; 

co ,_ 

co 

z ~ 0 u ::;: 1-

0 0 

8 8 

15 15 

15 15 

15 15 

0 0 

15 15 

0 0 

15 15 

15 15 

15 15 

0 0 

15 15 

15 15 

15 15 

15 15 

173 173 

Prot--' - - 11 A, 

";-

N 

N 

"';-

N 

a; 

'L? 

(/) 

c( 

0 

N X 

o; ,._ 

:h C) U') 

<( u 0 1-

0 0 

8 8 

15 15 

15 15 

15 15 

0 0 

15 15 

0 0 

15 15 

15 15 

15 15 

0 0 

15 15 

15 15 

15 15 

15 15 

173 173 

- -- bv PIP and Stat 

";-

,_ N N C) 

U') ch a> 

u <( 0 'L? 

X X (/) <( 

,_ ,_ ,_ 0 r-,_ c; c; c; X ON co ,_ co co ,_ CON 

co C) co co ~ co- z U') z z zen 

0 u 0 0 u o'L? :::;; 1- :::;; :::;; 1- :::;;~ )( )( )( )( X xo 

.... .... .... .... ;s; '<I" X (") (") M M <">,_ C) C) C) C) 

a> 

co 

a> 

co co 

a> 

co 

o> ,._ 

N ' co ~ ~~ z z z ZN z z<:> 0 0 0 o- 0 01-

:::;; :::;; :::;; :::;;~ :::;; :::i! X 

0 0 0 0 0 0 

8 40 12 8 8 8 

15 15 15 15 15 15 

15 15 15 15 15 15 

15 15 15 15 15 15 

0 0 0 0 0 0 

15 15 15 15 15 15 

0 0 0 0 0 0 

15 15 15 15 15 15 

15 15 15 15 15 15 

15 15 15 15 15 15 

0 0 0 0 0 0 

15 15 15 15 15 15 

15 15 15 15 15 15 

15 15 15 15 15 15 

15 15 15 15 15 15 

173 205 177 173 173 173 L-~~~---L~~~~~~--~~~~~~-= ~~ Note: P1= Protocol 1 (breeding and associated data); P2=Protocol2 (characterization) 

UNREQISTERED PIPS: 

";- X ,_ N ,_ C) N C) U') U') 

u o; 'L? -u 1- 1-

X (/) X ~ <( ~ 0 

N - X 

.... .... 

,_ o; ,_ ,_ ,_ 

C) U') co C) co 

U') ch z U') z u ~ 0 u 0 1- :::;; 1- :::;; 

X X X X X ,._ a. 

-' - - - .... - ..,. . <( -' a: ; ; ; (") - MN g 

~ .... a"' 

b ,_ ..... ,._ ,_ "'~ -' 

co co co co co co~ ~ 

Iii 1- z z z z z Z' 0 0 0 0 0 0 0(/) co ::> ::> 1- 

(/) :::;; :::;; :::;; :::;; :::;; :::;;~ (/) 

0 0 0 0 0 0 0 0 0 

124 8 8 20 8 8 8 60 184 

165 15 15 15 15 15 15 90 255 

165 15 15 15 15 15 15 90 255 

165 15 15 15 15 15 15 90 255 

0 0 0 0 0 0 0 0 0 

165 15 15 15 15 15 15 90 255 

0 0 0 0 0 0 0 0 0 

165 15 15 15 15 15 15 90 255 

165 15 15 15 15 15 15 90 255 

165 15 15 15 15 15 15 90 255 

0 0 0 0 0 0 0 0 0 

165 15 15 15 15 15 15 90 255 

165 15 15 15 15 15 15 90 255 

165 15 15 15 15 15 15 90 255 

165 15 15 15 15 15 15 90 255 

1,939 173 173 185 173 173 173 1050 2989 
APPENDIX 1.8 - --

REGISTERED PIPS: 

AR 

H 

ll 

IN 

lA 

KS 

MN 

MO 

NE 

NO 

OH 

PA 

PR 

so 

TX 

w 

I 

I 

Tota 

v 

M 

C) 

"' co 

z 

0 

:::;; 

2 

18 

12 

11 

11 

2 

12 

2 

13 

8 

8 

2 

20 

12 

10 

12 

155 

,..._ 

c; 

.., ,._ 

co C) 

z .,., 

0 u :::;; t2 2 

18 18 

8 11 

8 11 

8 11 

2 2 

8 11 

2 2 

8 12 

8 8 

8 8 

2 2 

20 20 

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6 9 

8 11 

124 149 

Prot' - -- - - 12A - . - -- bv -- PIP and - -- -- Sta - ----- t' 

"' ";- ,._ N N c;; N C) 

.,., "' u "' ch OL;> £3 (f) )( 

,._ ,._ < )( 0 ,._ ,._ ~ c; ,._ )( ~ 0 ";- C) 0 oN .., ,._ .., ,._ ~ """" .., :i'l .., .., C) .., ~ a; z z "' <0 .,., 

OL;> :::;; 0 u 0 0 z u ~~ t- :::;; t- :::;;< (f) )( )( )( ::;: )( xO ~ < X 0 v v 'd' ~ ~ ~ .. N <") M M M M ,._ 

a; )( a: a: C) 0 C) oo ...... "' "' a> a>.,., .,., 0 co .., .., cO co co~ ch "' z z z z z zu < u 0 0 0 0 0 ot- 0 t- :::;; :::;; :::;; ::;:,_ :::;; :::;; )( 

2 2 2 4 2 2 2 2 

18 19 19 19 19 19 19 19 

20 17 17 16 14 17 16 17 

20 17 17 19 17 17 16 17 

20 17 17 19 17 17 16 17 

4 4 4 4 4 4 4 4 

18 17 17 16 14 17 16 17 

5 3 3 4 3 3 3 3 

20 17 17 16 14 17 16 17 

10 9 9 16 9 9 9 9 

10 9 9 16 9 9 9 9 

2 2 2 4 2 2 2 2 

20 20 20 20 20 20 20 20 

15 14 14 16 11 14 13 14 

10 9 9 12 6 9 8 9 

15 14 14 16 11 14 13 14 

209 190 190 217 172 190 182 190 L_ ~~~~~~

~~~~~~~~~~~~~~~~~~ Note: P1- Protocol1 (breeding and associated data); P2=Protocol2 (characterization) 

UNREGISTERED PIPS: 

";- )( ,._ N ,._ C) N C) .,., ~ .,., 

u "' .,., u t- ch t-

";- )( < )( 

N ~ 0 ~ N 

a; "¢ )( .... ,._ ,._ ,._ ,_ C) .,., .., C) .., 

.,., ch z .,., z 

u < 0 u 0 t-

)( 

0 

X 

:::;; 

X 

t-

)( 

:::;; 

)( ,._ a. 

..-1 ~ ... ... 'o:t ... v' ..-1 a: 

g ~ ~ ~ M ~ """" ~ 

C) a"' ,._ ..-1 

...... ,._ m ...... a>~ cO cO cO co <0 <0~ g 

~ 

t- z z z z z Z' 

:::> 

CD 0 0 0 0 0 0(/) CD :::> 0 

(f) :::;; :::;; :::;; :::;; :::;; :::;:;3 (f) I- 

24 2 2 2 2 2 2 12 10 

205 18 18 19 19 19 19 112 28 

165 18 10 18 16 16 38 116 58 

170 25 11 50 20 17 50 173 68 

170 30 11 50 20 17 50 178 63 

38 4 2 4 4 4 4 22 18 

163 23 10 18 16 16 38 121 58 

33 5 2 3 3 3 3 19 18 

167 24 10 18 16 16 38 122 43 

104 9 8 9 9 9 9 53 28 

104 9 8 9 9 9 9 53 28 

24 2 2 2 2 2 2 12 10 

220 20 20 20 20 20 20 120 40 

142 20 10 15 13 13 35 106 38 

97 15 8 10 8 8 30 79 40 

142 20 10 15 13 13 35 106 43 

1,968 244 142 262 190 184 382 1404 591 

-------

-- 
APPENDIX II.A 

AR 

HI 

IL 

IN 

lA 

KS 

MN 

MO 

NE 

NO 

OH 

PA 

PR 

so 

TX 

WI 

Total 

Cry1A.105 

0 

5,076 

6,345 

6,345 

6,345 

0 

6,345 

0 

6,345 

6,345 

6,345 

0 

6,345 

6,345 

6,345 

6,345 

74,871 

Amount of Seed (lbs) Expected to Be Used 

for Each Protein for Protocol 1 Acres 

Pounds of PIP-Containing Seed 

Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35Ab1 

0 0 0 0 0 

5,076 .2.820 5,452 3,572 3,572 

6,345 4,230 7,050 5,640 5,640 

6,345 4,230 7,050 5,640 5,640 

6,345 4,230 7,050 5,640 5,640 

0 0 0 0 0 

6,345 4,230 7,050 5,640 5,640 

0 0 0 0 0 

6.345 4,230 7,050 5,640 5,640 

6,345 4,230 7,050 5,640 5,640 

6,345 4,230 7,050 5,640 5,640 

0 0 0 0 0 

6,345 4,230 7,050 5,640 5,640 

6,345 4,230 7,050 5,640 5,640 

6,345 4,230 7,050 5,640 5,640 

6,345 4,230 7,050 5,640 5,640 

74,871 49,350 83,002 65,612 65,612 

Note: P1= Protocol1 (breed1ng and associated data); P2=Protocol2 (charactenzatlon) 

APPENDIX 11 .8 

AR 

HI 

IL 

IN 

lA 

KS 

MN 

MO 

NE 

NO 

OH 

PA 

PR 

so 

TX 

WI 

Total 

Cry1A.105 

940 

7,990 

7,661 

8,648 

8,648 

1,598 

7,661 

1,269 

7,708 

4,089 

4,089 

940 

8,460 

6,674 

4,747 

6,674 

87,796 

Amount of Seed (lbs) Expected to Be Used 

for Each Protein for Protocol 2 Acres 

Pounds of PIP-Containing Seed 

Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35Ab1 

940 564 1,034 752 752 

7,990 5,264 8,836 . 7,097 7,097 

7,661 5,452 8,037 7,473 7,473 

8,648 8,131 9,165 9,588 9,588 

8,648 8,366 9,165 9,588 9,588 

1,598 1,034 1,692 1,504 1,504 

7,661 5,687 8,037 7,379 7,379 

1,269 893 1,363 1,222 1,222 

7,708 5,734 8,084 7,473 7,473 

4,089 2,491 4,465 3,431 3,431 

4.089 2,491 4,465 3,431 3,431 

940 564 1,034 752 752 

8,460 5,640 9,400 7,520 7,520 

6,674 4,982 7,050 6,251 6.251 

4,747 3,713 5,029 4,371 4,371 

6,674 4,982 7,050 6,251 6,251 

87,796 65,988 93,906 84,083 84,083 

Note: P1- Protocol1 (breedmg and assoc1ated data), P2-Protocol2 - (charactenzatlon) 

Cry3b1 

0 

4,888 

7,755 

7,755 

7,755 

0 

7,755 

0 

7,755 

7,755 

7,755 

0 

7,755 

7,755 

7,755 

7,755 

90,193 

Cry3b1 

1,034 

9,682 

~.883 

11,703 

11 ,938 

1,880 

9,118 

1,551 

9,165 

4,559 

4,559 

1,034 

10,340 

7,849 

5,546 

7,849 

106,690 

DER-10 
APPENDIX II I. A Amount of PIP Protein (lbs, g) Expected to Be Used for Protocol1 Acres 

Pounds of Protein 

Cry1A.105 Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35 Ab1 Cry3b1 

AR lbs: 0 0 0 0 0 0 0 

g: 0 0 0 0 0 0 0 

HI lbs: 0.03553 0.01066 4.794E-08 0.04034 0.3358 0.008216 0.06101 

g: 16.12 4.835 2.175E-05 18.30 152.3 3.727 27.67 

IL lbs: 0.04442 0.01332 7.191 E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

IN lbs: 0.04442 0.01332 7.191 E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

lA lbs: 0.04442 0.01332 7.191 E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

KS lbs: 0 0 0 0 0 0 0 

g: 0 0 0 0 0 0 0 

MN lbs: 0.04442 0.01332 7.191E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

MO lbs: 0 0 0 0 0 0 0 

g: 0 0 0 0 0 0 0 

NE lbs: 0.04442 0.01332 7.191 E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

NO lbs: 0.04442 0.01332 7.191 E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

OH lbs: 0.04442 0.01332 7.191E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

PA lbs: 0 0 0 0 0 0 0 

g: 0 0 0 0 0 0 0 

PR lbs: 0.04442 0.01332 7.191 E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

so lbs: 0.04442 0.01332 7.1 91E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

TX lbs: 0.04442 0.01332 7.191E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

WI lbs: 0.04442 0.01332 7.191 E-08 0.05217 0.5302 0.01297 0.1008 

g: 20.15 6.044 3.262E-05 23.66 240.5 5.884 45.73 

TOT lbs: 0.5241 0.1572 8.390E-07 0.6142 6.168 0.1509 1.170 

g: 237.7 71 .32 3.805E-04 278.6 2,798 68.45 530.7 

Note: P1- Protocol1 (breedmg and assoctated data); P2-Protocol 2 (charactenzallon) 

DER- 11 
APPENDIX II 1.8 Amount of PIP Protein (lbs, g) Expected to Be Used for Protocol1 Acres 

Pounds of Protein 

Cry1A.105 Cry2Ab2 Dvsnf7 Cry1F Cry34Ab1 Cry35 Ab1 Cry3b1 

AR lbs: 0.006580 0.001974 9.588E-09 0.007652 0.07069 0.001730 0.01344 

g: 2.985 0.8954 4.349E-06 3.471 32.06 0.7845 6.097 

HI lbs: 0.05593 0.01678 8.949E-08 0.06539 0.6671 0.01632 0.1 261 

g: 25.37 7.611 4.059E-05 29.66 302.6 7.404 57.22 

IL lbs: 0.05363 0.01609 9.268E-08 0.05947 0.7025 0.01719 0.1055 

g: 24.32 7.297 4.204E-05 26.98 318.6 7.796 47.84 

IN lbs: 0.06054 0.01816 1.382E-07 0.06782 0.9013 0.02205 0.1233 

g: 27.46 8.238 6.270E-05 30.76 408.8 10.00 55.93 

lA lbs: 0.06054 0.01816 1.422E-07 0.06782 0.9013 0.02205 0.1246 

g: 27.46 8.238 6.451E-05 30.76 408.8 10.00 56.52 

KS lbs: 0.01119 0.003356 1.758E-08 0.01252 0.1414 0.003459 0.02430 

g: 5.074 1.522 7.973E-06 5.679 64.13 1.569 11 .02 

MN lbs: 0.05363 0.01609 9.668E-08 0.05947 0.6936 0.01697 0.1068 

g: 24.32 7.297 4.385E-05 26.98 314.6 7.698 48.43 

MO lbs: 0.008883 0.002665 1.518E-08 0.01009 0.1149 0.002811 0.01939 

g: 4.029 1.209 6.886E-06 4.575 52.10 1.275 8.794 

NE lbs: 0.05396 0.01619 9.748E-08 0.05982 0.7025 0.01719 0.1 070 

g: 24.47 7.342 4.422E-05 27.13 318.6 7.796 48.54 

NO lbs: 0.02862 0.008587 4.235E-08 0.03304 0.3225 0.007891 0.05920 

g: 12.98 3.895 1.921E-05 14.99 146.3 3.579 26.85 

OH lbs: 0.02862 0.008587 4.235E-08 0.03304 0.3225 0.007891 0.05920 

g: 12.98 3.895 1.921E-05 14.99 146.3 3.579 26.85 

PA lbs: 0.006580 0.001974 9.588E-09 0.007652 0.07069 0.001730 0.01344 

g: 2.985 0.8954 4.349E-06 3.471 32.06 0.7845 6.097 

PR lbs: 0.05922 0.01777 9.588E-08 0.06956 0.7069 0.01730 0.1344 

g: 26.86 8.059 4.349E-05 31 .55 320.6 7.845 60.97 

so lbs: 0.04672 0.01402 8.469E-08 0.05217 0.5876 0.01 438 0.09048 

g: 21 .19 6.357 3.842E-05 23.66 266.5 6.521 41 .04 

TX lbs: 0.03323 0.009969 6.312E-08 0.03721 0.4109 0.01005 0.06075 

g: 15.07 4.522 2.863E-05 16.88 186.4 4.560 27.55 

WI lbs: 0.04672 0.01402 8.469E-08 0.05217 0.5876 0.01438 0.09048 

g: 21 .19 6.357 3.842E-05 23.66 266.5 6.521 41.04 

TOT lbs: 0.6146 0.1844 1.122E-06 0.6949 7.904 0.1934 1.258 

g: 278.8 83.63 5.088E-04 315.2 3,585 87.72 570.8 

Note: P1- Protocol 1 (breedrng and assocrated data), P2- Protocol2 (charactenzatron} 

DER- 12