# Patent

## Title
Virus-expressing host costimulation molecules

## Abstract

An antiviral vaccine that encodes costimulation molecules for increasing immune response to the particular virus at issue is provided herein. In an illustrative embodiment, in mice lacking both B7-1 and B7-2 (B7KO), the increased severity of HSV infection has confirmed the importance of these molecules in generation of HSV-specific immunity. To test the concept that B7-1 or B7-2 expression by replication-defective HSV could augment its immunogenicity and protective capacity, the present inventors constructed replication-defective HSV-2 encoding B7-1 or B7-2. Both viruses partially reconstituted immune responses to HSV compared with replication-defective virus alone when used to immunize B7KO mice, indicating that the increased responsiveness to virus could be attributed to virus-encoded B7 molecules.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a nonprovisional of and claims the benefit of U.S. Provisional Application Ser. No. 61/078,196, filed Jul. 3, 2008, which document is hereby incorporated by reference to the extent permitted by law.


BACKGROUND OF THE INVENTION
Herpes simplex virus 1 (HSV-1) infections are ubiquitous in the population world-wide, and 65% of the United States population is infected before age 50. Herpes simplex virus 1 (HSV-1) remains a prevalent cause of eye infections, afflicting 450,000 persons in the United States. HSV-mediated ocular diseases include blepharitis, conjunctivitis, and stromal keratitis. Periodic reactivations in infected persons can cause recurrent disease of the cornea. For some, the reactivations lead to corneal scaring and loss of vision; herpetic stromal keratitis is the second most common cause of non-traumatic corneal blindness. Development of an effective vaccine against HSV-1 would help control or prevent this sight-threatening disease.
Moreover, herpes simplex virus types 1 and 2 perpetrate most genital ulcerative disease. Approximately 17% of individuals in the United States are infected with HSV-2, and up to 75% world-wide. HSV-2 infects primarily the genital epithelium where foci of replication cause vesicles to form and ulcerate. The virus also rapidly ascends sensory nerve fibers terminating in the mucosa and enters a latent state in the sensory nerve ganglia, from which it periodically reactivates and travels intra-axonally back to the mucosal epithelium to cause asymptomatic shedding or recurrent disease. Frequency and severity of recurrences reflects the extent of primary and latent infection.
HSV-2 infections typically are sexually transmitted, but also may be transmitted to babies born to HSV-infected women who undergo peripartum primary infection or reactivation. In newborns, the infection often widely disseminates, causing sometimes fatal disease and leaving survivors with long-term sequelae. Vaccines to prevent or treat HSV-2 infections have been sought for decades to alleviate the disease burden. One adjuvanted gD2 glycoprotein preparation has shown some promise, but its efficacy is limited to HSV-seronegative women. Methods to improve current vaccines under development, or new approaches that combine safety with superior efficacy are needed.
The T-cell response to HSV is thought critical to the effective control of infection. Induction of naïve T-cell responses requires three signals: T-cell receptor engagement of the appropriate antigen/MHC molecule, interaction of CD28 with B7-1 and B7-2 costimulation molecules, and cytokines that drive differentiation. Antiviral vaccines, like viruses, must also elicit or provide these same signals in order to induce strong T-cell responses. Some types of vaccine-containing viral glycoprotein or peptide epitopes provide only signal one and are often mixed with adjuvant in order to provide the “danger signals” necessary to elicit signals two and three. DNA vaccines provide signal one to T-cells, but amplify the signal through gene expression in vivo and synthesis of antigen in a form that particularly stimulates CD8 T-cells. Vaccine prototypes consisting of HSV glycoproteins or immunodominant peptide epitopes in adjuvant or plasmid-encoding HSV-1 gD can decrease corneal shedding of HSV-1 and reduce herpes stromal keratitis. Vaccine preparations consisting of or encoding multiple glycoproteins are more potent than a single glycoprotein indicating the benefits of a multivalent vaccine.
Attenuated by replication-competent viruses as vaccines naturally provoke T-cell responses by virtue of their similarity to infection with wild-type virus strains. They also encode numerous external and internal viral proteins that act as targets for immune recognition. Neuroattenuated and single cycle gH mutants of HSV-1 have been explored as potential vaccines for prevented eye disease with success in reducing viral replication and HSV-mediated corneal disease. These forms of replication-competent vaccine may further augment and guide the immune response by encoding cytokines (signal three). For example, a LAT−g34.5− HSV-1 expressing two copies of IL-12p35 improved T-cell activation and elicited higher neutralizing antibody titers than virus without IL-12. These responses correlated with better efficacy against ocular virus replication and establishment of latency. However, titers of LAT−g34.5− HSV-1 are still amplified 10,000-fold in tissue culture, raising concern about the safety of such replication-competent agents.
In answer to the needs for both safety and immunogenicity in a vaccine, replication-defective viruses have also been explored as a means to prevent HSV-1 infection and HSV-mediated eye disease. HSV-1 strains made replication-defective by disruption of the UL29 gene encoding ICP8, essential for viral DNA replication, have shown promise in a mouse model of corneal infection. A single immunization with ICP8− virus reduces HSV-1 replication in the cornea after challenge. Immunization with replication-defective virus also reduces acute and latent infection of the trigeminal ganglia (TG) and incidence of HSK. Replication-defective HSV-1 induces T-cell proliferation and CD8 T-cell responses. CD8 T-cells may protect against immunopathologic damage to the cornea following HSV infection while CD4 T-cells reduce virus replication in the cornea and latent infection in the TG. This basic replication-defective virus has undergone further modification. The virion host shutoff (vhs) protein encoded by UL41 has known immune evasion properties, and immunization of mice with an ICP8− mutant virus increased protection against replication, disease and latency after corneal challenge. Further manipulation of the viral genome may yield additional benefits to immunogenicity and protective capacity of the replication-defective viruses.
SUMMARY OF THE INVENTION
In one of many illustrative, non-limiting aspects of the present invention, there is provided a novel approach for increasing immune response to antiviral vaccines though recombinant DNA technology. More particularly, certain embodiments of the present invention provide an antiviral vaccine that encodes costimulation molecules for increasing immune response to the particular virus at issue. Other objects and features will be in part apparent and in part pointed out hereinafter.



BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING
The accompanying drawing forms a part of this specification and is to be read in conjunction therewith:
FIG. 1A is a schematic representation of a Southern blot analysis of the tk locus wherein the genomic position of the tk ORF is shown on line 1, an expanded view of this region (line 2) shows the location of EcoRI restriction enzyme sites, the hatched bar depicts the probe fragment used for Southern blot analysis, and line 3 shows the insertion cassette containing the HCMV IEp fused to either the B7-1 (Δ41Δ29B7-1) or B7-2 (Δ41Δ29B7-2) ORF, each of which contains an EcoRI site near the carboxyl terminus;
FIG. 1B is the Southern blot analysis of the tk locus of FIG. 1A wherein genomic DNAs isolated from the Δ41Δ29 parental and recombinant viruses were digested with EcoRI, subjected to electrophoresis, and transferred to membrane, the blot was hybridized to a 32P-labeled fragment of p101086.7 DNA, and the expected sizes of the EcoRI fragments were 2416 bp for Δ41Δ29 (lane 1), 3242 bp for B7-1 virus (lane 2), and 2923 bp for B7-2 virus (lane 3);
FIG. 2 shows B7 molecule expression on the surface of cells infected in vitro with Δ41Δ29B7-1 or Δ41Δ29B7-2 viruses wherein S2 cell monolayers were mock infected or infected at MOI 5 and collected and stained 24 h later with rabbit anti-HSV-2 followed by goat anti-rabbit-PE, and with the appropriate anti-B7-biotin antibody followed by streptavidin-FITC and the cells were analyzed by flow cytometry as follows: A) Mock-infected cells stained with anti-B7-1 and anti-HSV; B) Δ41Δ29B7-1-infected cells stained with anti-B7-1; C) Δ41Δ29-infected cells stained with anti-HSV; D) Δ41Δ29-infected cells stained with anti-B7-1 and anti-HSV-1; E) Δ41Δ29B7-1-infected cells stained with anti-B7-1 and anti-HSV-1; and F) Δ41Δ29B7-2-infected cells stained with anti-B7-2 and anti-HSV-1;
FIG. 3A is a bar chart representation of HSV-specific CD8 T-cell responses induced by immunization wherein: groups of BALB.B mice were immunized with 4×105 pfu of the indicated replication-defective virus or control supernatant; six days after immunization cells from the pooled draining lymph nodes were isolated; and cells were stimulated in vitro with various concentrations of gB498-505 peptide and analyzed in an IFNγ ELISpot assay; and data are the average of duplicate wells;
FIG. 3B shows the HSV-specific CD8 T-cell responses induced by immunization of FIG. 3A wherein cells were stained with CD8 and the KbgB498-505 tetramer and analyzed by flow cytometry, gates were set on CD8+ cells and then analyzed for tetramer staining, and the percentage of CD8+ T-cells that are tetramer-positive is shown for mice immunized with A) control supe, or B) Δ41Δ29, C) B7-1+ and D) B7-2+ viruses;
FIG. 4 is a bar chart representation of pre-challenge HSV-1-specific serum IgG titers wherein groups of BALB/c mice were immunized with low, medium or high doses of the indicated viruses, blood was collected 21 d after immunization and titer of HSV-specific IgG was determined by ELISA, the data represent the geometric mean+SEM compiled from 2 independent experiments (n=10-12);
FIG. 5 is a graphical representation of the titer of challenge virus shed from the corneal epithelium wherein groups of BALB/c mice were immunized with A) high, B) medium or C) low doses of the indicated virus or control supernatant, all groups were challenged 1 mo after immunization by inoculation of HSV-1 mP onto the corneas and mouse eyes were swabbed at the indicated times post-challenge, titers of virus collected on swabs were determined by standard plaque assay, and the data represent the geometric mean+SEM for 10 to 12 samples compiled from 2 independent experiments. *, P=0.002-0.014;
FIG. 6 is a graphical representation of the severity of blepharitis post-challenge wherein mice were immunized with the A) high dose, B) medium dose, or C) low dose of the indicated virus or control supernatant and challenged as described in FIG. 5, blepharitis was scored daily after challenge in masked fashion, and the data represent the mean+SEM for all mice compiled from 2 independent experiments (n=20 eyes for control, and 24 to 30 eyes for virus-immunized mice);
FIG. 7 is a bar chart representation of the incidence of severe keratitis wherein eyes of mice were scored in masked fashion for signs of keratitis 9 d and 14 d post-challenge and the proportion of eyes with severe (sight-damaging) keratitis is shown for groups originally immunized with the A) high dose, B) medium dose, or C) low dose of the indicated virus or control supernatant (n=20 to 30 eyes day 9, 14 to 30 eyes day 14). **, P=0.0003 to <0.0001; *, P=0.0162;
FIG. 8 is a graphical representation of the survival of immunized mice after challenge with HSV-1 wherein the same mice that were immunized and challenged as FIG. 5 were monitored daily for survival, mice were immunized with the low dose of the indicated virus or control supernatant, and the data represent the percentage surviving out of 10 control or 14 to 15 virus-immunized mice per group from 2 independent experiments;
FIG. 9 is a bar chart representation of the relative levels of HSV-1 DNA in trigeminal ganglia during latency wherein: groups of mice immunized with the medium dose of virus or control supernatant were challenged with 8×105 pfu HSV-1 mP 4 wk later; one month after challenge, trigeminal ganglia were removed and DNA was extracted; relative viral DNA content was assessed by real-time PCR using primers for UL50 after normalization of signal to GAPDH and wherein: A) Data represent the relative mean fold decrease (+SD) of latent genome in 11 TG from Δ41Δ29B7-1- and Δ41Δ29B7-2-immunized mice compared with 11 TG from Δ41Δ29-immunized mice (set to 1), P>0.05 by ANOVA; and B) Data represent the relative mean fold decrease (+SD) of latent genome in 11 TG from each group of immunized mice compared with 2 TG, from a mouse immunized with control supernatant that survived the challenge (set to 1); P<0.001 by ANOVA, P<0.001 for each virus-immunized group compared with the control supernatant group by Dunnett t test; and
FIG. 10 is a bar chart representation of acute replication of challenge virus in the nervous system wherein mice were immunized with the medium dose of the indicated virus or with control supernatant and challenged by the corneal route one month later, after 3 days mice were sacrificed and trigeminal ganglia and brainstems were dissected, homogenized, and virus titer in them was determined by standard plaque assay, and the data represent the geometric mean+SEM for 10 trigem and 5 brainstem samples per group, *P=0.0337 for B7-2 compared with Δ41Δ29; (P<0.001 for all 3 vaccine virus trigem samples compared with control supernatant; P=0.0045 for B7-2 brainstem sample compared with control supernatant).



DETAILED DESCRIPTION OF THE INVENTION
The present invention generally relates to a novel approach to augment the immune response of a vertebrate host to a virus using an antiviral vaccine or other therapeutic or prophylactic composition that encodes or expresses costimulation molecules within its genome. The present invention is generally applicable to any type of attenuated and replication-defective viral host and antigens and epitopes thereof, including, but not limited to, caudovirales, herpesvirales, mononegavirales, nidovirales, and picornavirales. Specific but non-limiting examples of such viruses include hepadnaviridae including hepatitis B virus (HBV), flaviviridae including human hepatitis C virus (HCV), yellow fever virus and dengue viruses, retroviridae including human immunodeficiency viruses (HIV) and human T lymphotropic viruses (HTLV1 and HTLV2), herpesviridae including herpes simplex viruses (HSV-1 and HSV-2), epstein barr virus (EBV), cytomegalovirus, varicella-zoster virus (VZV), human herpes virus 6 (HHV-6), human herpes virus 8 (HHV-8), herpes B virus, papovaviridae including human papilloma viruses, rhabdoviridae including rabies virus, paramyxoviridae including respiratory syncytial virus, reovitidae including rotaviruses; bunyaviridae including hantaviruses, filoviridae including ebola virus, adenoviriidae, parvoviridae including parvovirus B-19, arenaviridae including lassa virus, orthomyxoviridae including influenza viruses, poxviridae including orf virus, molluscum contageosum virus and monkey pox virus, togaviridae, coronaviridae including corona viruses, and picornaviridae. For the purposes of illustration only, the present invention will be described in connection with herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2) in mice, but one skilled in the art will appreciate that the methods and compositions described herein may be applied to any virus and vertebrate suitable for use in the present invention.
A viable vaccine candidate must meet goals of both safety and efficacy. While attenuated vaccines may be used in the present invention, replication-defective vaccines are often considered to be the safest form of live virus vaccine because they do not reproduce and spread in the recipient. Replication-defective vaccines also express numerous viral proteins in infected cells that stimulate broad spectrum antiviral immunity and further manipulation of prototype replication-defective virus vaccines may enhance their immunogenicity and effectiveness. Moreover, an optimal immune response to an anti-viral vaccine requires viral antigens to be presented in a context in which ample costimulation occurs. For the purposes of illustration only, the following disclosure is presented using B7 costimulatory molecules; however, it will be appreciated by one skilled in the art that any suitable costimulation molecule may be used in the present invention.
In mice lacking both B7-1 and B7-2 (B7KO), the increased severity of HSV infection has confirmed the importance of these molecules in generation of HSV-specific immunity. To test the concept that B7-1 or B7-2 expression by replication-defective HSV could augment its immunogenicity and protective capacity, the present inventors constructed replication-defective HSV-2 encoding B7-1 or B7-2. Both viruses partially reconstituted immune responses to HSV compared with replication-defective virus alone when used to immunize B7KO mice, indicating that the increased responsiveness to virus could be attributed to virus-encoded B7 molecules. Furthermore, replication-defective HSV-2 encoding B7-2 conferred better protections against HSV-2 infection to wild-type mice than did the parent replication-defective virus even though wild-type mice express endogenous B7 molecules. To determine whether provision of additional “signal two” could improve the immunogenicity and capacity of ICP8−vhs− HSV-1 to protect against HSK, the present inventors constructed and tested ICP8−vhs− viruses that encode B7 costimulation molecules within their genome.
B7 costimulation molecules, encoded by the replication-defective, vhs-virus help the vaccine strain achieve greater protection of mice from stromal keratitis induced by HSV-1 challenge than vhs-, replication-defective virus lacking B7. Virus-encoded B7-1 and B7-2 equally improved vaccine-mediated protection from keratitis, and this protection could be observed using a dose of only 4×104 pfu of cell-free replication-defective virus. The increased efficacy of B7-expressing viruses correlated with enhanced, virus-specific CD4 and CD8 T-cell responses. B7-2-expressing virus also protected the CNS from acute infection significantly better than virus lacking B7. Both B7-1 and B7-2-expressing viruses showed a distinct, though not statistically significant, trend toward improving blepharitis, latent viral genome load and survival. Thus, provision of signal two for T-cells by the HSV-1 vaccine strain enhanced protection from keratitis mediated by HSV-1 itself.
In one embodiment of the present invention, deletion of from a replication-defective vaccine strain of HSV-1 amplified immune responses and enhanced its capacity to protect against corneal infection with virulent HSV-1. The method of the present invention including the expression of expressing host costimulation molecules from the genome of a replication-defective HSV-1 vaccine achieved even greater protection against HSV-1-induced keratitis, in some manner by providing signal two on the virus-infected cell to responding T-cells.
B7-1 and B7-2 costimulation enhances numerous aspects of the antiviral immune response, including enhancement of cytokine production, proliferation, cytotoxicity, and antibody production. Conversely, when B7-2 is blocked by specific antibody treatment, CD8 T-cell cytotoxic activity declines and HSV-1 corneal infection worsens. The critical signals mediated by B7-1 and B7-2 operate at different temporal phases of T-cell activation. B7-2 is constitutively expressed and rapidly upregulated, whereas B7-1 expression on professional APC must be provoked. Thus, the present inventors anticipated that B7-2 might stimulate stronger immunity and achieve better protective efficacy than the virus encoding B7-1. However, B7-1 and B7-2-expressing viruses generated similar levels of protection from HSV-1 corneal infection and this was reflected in equivalent levels of virus-specific antibody and T-cell responses.
Precedent exists for the beneficial activity of virus-encoded B7 costimulation molecules as a strategic element of vaccines. B7-1 and B7-2 encoded by vaccinia or adenovirus vectors markedly augment immunogenicity of coexpressed tumor antigens, and help reduce tumor burden in animal models. Our viruses encoding B7 costimulation molecules represent a new direction in that they enhance the immune response to the pathogen itself. Noninfectious HSV particles engineered to contain B7 costimulation molecules on their surface also induce stronger immune responses than particles without, supporting the idea of providing B7 costimulation molecules in conjunction with virus antigens to artificially create an antigen presenting cell. ICP8− HSV-2 expressing B7-2 and ICP8− HSV-1 additionally lacking vhs are each highly immunogenic. Indeed, a great deal of protection could be achieved with a single, low immunizing dose. With ICP8−vhs−B7+ HSV-1, the present inventors established a lower limit of immune effector activity in BALB/c mice with the dose of 4×103 pfu. This dose generated immune responses that still reduced replication in the corneal epithelium by 4 days post-infection and improved the survival rate, but did not significantly impact the incidence or severity of blepharitis or keratitis. By increasing the dose to just 1×104 pfu, significant protection against HSK was also achieved. Therefore, as used herein, administration of an “effective amount” of the virus-expressing host costimulation molecules hereof is defined as an amount effective, at dosages and for periods of time necessary to achieve the desired result. The effective amount of the virus-expressing host costimulation molecules of the invention may vary according to factors such as the disease state, age, sex, and weight of the animal. Dosage regima may be adjusted to provide the optimum therapeutic response. For example, several divided doses may be administered daily or the dose may be proportionally reduced as indicated by the exigencies of the therapeutic situation.
Thus, the present invention includes pharmaceutical compositions containing virus-expressing host costimulation molecules for use in enhancing an immune response. Such pharmaceutical compositions can be for intralesional, intravenous, topical, rectal, parenteral, local, inhalant or subcutaneous, intradermal, intramuscular, intrathecal, transperitoneal, oral, and intracerebral use. The composition can be in liquid, solid or semisolid form, for example pills, tablets, creams, gelatin capsules, capsules, suppositories, soft gelatin capsules, gels, membranes, tubelets, solutions or suspensions. The pharmaceutical compositions of the invention can be intended for administration to vertebrates. Dosages to be administered depend on individual needs, on the desired effect and on the chosen route of administration. The pharmaceutical compositions can be prepared by per se known methods for the preparation of pharmaceutically acceptable compositions which can be administered to patients, and such that an effective quantity of the active substance is combined in a mixture with a pharmaceutically acceptable vehicle. On this basis, the pharmaceutical compositions include, albeit not exclusively, the active compound or substance in association with one or more pharmaceutically acceptable vehicles or diluents, and contained in buffered solutions with a suitable pH and iso-osmotic with the physiological fluids. The pharmaceutical compositions may additionally contain other immunostimulatory agents to enhance the immune response.
Example 1
Cells and viruses. The replication-defective mutant of HSV-1 KOS, Δ41Δ29 has defects in expression of vhs and the essential gene product ICP8 due to insertion of a nonsense linker in the UL41 open reading frame (ORF) at amino acid position 238 and disruption of the UL29 ORF by insertion of a lacZ expression cassette, respectively. Δ41Δ29 was propagated in S2 cells, a Vero cell line stably expressing ICP. Δ41Δ29 was further mutated to contain a murine B7-1 (CD80) or B7-2 (CD86) expression cassette. CD80 and CD86 open reading frames cloned downstream of the HCMV immediate early enhancer/promoter in plasmids pBS(HCMV/B7-1) and pEH48(HCMV/B7-2) were excised and inserted into a BglII site previously engineered 751 bp from the 5′ end of the thymidine kinase (tk) ORF in plasmid p101086.7BglII. These plasmids were cotransfected with full-length Δ41Δ29 DNA into S2 cells using nucleofection (Amaxa Biosystems) according to the manufacturer's protocol. To select B7-expressing recombinant viruses, S2 cells infected with virus progeny of the cotransfection were incubated in the presence of 100 μM acyclovir. Potential recombinant viruses able to grow in the presence of acyclovir were grouped in pools. Fresh cells infected with each pool were screened by flow cytometry for expression of B7 molecules. Isolates from positive pools were individually re-screened by flow cytometry and then triply plaque-purified. Insertion into tk was confirmed by Southern blot analysis. The B7-1- and B7-2-expressing viruses were named Δ41Δ29B7-1 and Δ41Δ29B7-2, respectively. Viruses used for immunizations were produced free of cell debris by isolation from the supernatant of infected cell monolayers using high speed centrifugation. HSV-1 strain microplaque (mP) was propagated in Vero cells. Virus titers were determined on S2 or Vero cells by standard plaque assay.
Mice. Female BALB/c mice were purchased from the National Cancer Institute. Female BALB.B mice (H-2b) were purchased from The Jackson Laboratories. All mice were housed at Saint Louis University under specific-pathogen-free conditions in accordance with institutional and federal guidelines, and were used at 6 weeks of age.
Flow cytofluorometric analyses. S2 cells infected with potential recombinant plaque isolates were stained 24 hr late by addition of anti-B7-1 or B7-2-biotin (1:150; PharMingen/Becton-Dickinson), followed by streptavidin-FITC (1:150; Immunotech) and analyzed by flow cytometry on a FACSCalibur. For demonstration of B7 expression by Δ41Δ29B7-1 and Δ41Δ29B7-2, S2 cells were stained 24 hr after infection at MOI of 5 by addition of anti-B7-1 or B7-2 biotin and anti-HSV-1 rabbit antiserum (1:100; Dako), followed by streptavidin-FITC and goat anti-rabbit-PE (1:100; Vector Laboratories) secondary reagents, respectively.
Southern blot hybridization. Viral DNAs were purified from potential recombinant viruses using a Qiagen QIAamp DNA Mini Kit according to the manufacturer's instructions. One μg of each DNA sample was subjected to EcoRI restriction digestion, and fragments were separated on a 0.8% agarose gel. DNA fragments were transferred to Hybond-N+ nylon membrane (Amersham) by capillary diffusion and hybridized to a randomly primed, [32P]-labeled SacI fragment of plasmid p101086.7 used as a probe. Images were obtained on X-ray film by autoradiography.
Immunization of mice. For immunization, hind flanks of mice were injected subcutaneously (s.c.) with 4×105 pfu (high), 4×104 pfu (medium), or 4×103 pfu (low) doses of virus suspended in 40 μl total vol of normal saline. Cohorts of mice received an equivalent amount of supernatant concentrated from uninfected cell cultures as a negative control for immunization.
Assays of T-cell response. CD8 T-cell responses induced by vaccination were compared by ELISpot assays and tetramer staining of BALB.B mice immunized as described above. For ELISpot assays, groups of BALB.B mice were immunized with 4×105 pfu (acute ELISpot and tetramer) or 4×104 pfu (recall tetramer) of the various vaccine strains or an equivalent amount of control supernatant s.c. in the hind flank. Draining lymph nodes were removed 6 d later and 3×106 cells were added per well in duplicate to Milliscreen-HA plates (Millipore) previously coated with antibody to IFNγ (BD Pharmingen). HSV-1 gB peptide 498-505 was added to the cultures at the indicated concentrations. After incubation for 20 hr, plates were washed extensively to remove cells and IFNγ captured on the membrane was detected using a biotinylated anti-IFNγ antibody (BD Pharmingen), followed by streptavidin conjugated to alkaline phosphatase (BDPharmingen) and BCIP-NBT (Sigma). Spots were counted using an Immunospot plate reader (v. 5.0; Cellular Technology, Ltd.).
For tetramer staining, 106 cells per sample were incubated in FcBlock (BD Biosciences) and stained with phycoerythrin-labeled Kb-gB498-505 tetramers at 1:100 for 20 min at 37° C. Cells were then washed, fixed and stained with FITC-labeled anti-CD8 clone CT-CD8a at 1:30 dilution on ice. Cells were observed by flow cytometry using an LSRII (Becton Dickinson) and analyzed using FloJo 8.0 software.
Quantitation of serum antibodies. To determine the titer of HSV-specific serum antibodies induced by vaccination, mice were unimmunized or immunized with 5BlacZ, 5B86 or control supernatant. Blood was collected from the tail vein of mice 21 days after immunization. Serum was prepared by clot retraction and analyzed by ELISA. Anti-mouse-IgG-biotin (R & D Systems, Minneapolis, Minn.) was used as secondary antibody and detected using streptavidin-HRP followed by OPD substrate (Sigma, St. Louis, Mo.). Plates were read at 490 nm on a BioRad 680 reader. Antibody titers were determined by comparison to standard curves generated with serum containing known concentrations of IgG captured on plates coated with goat-anti-kappa light chain antibody (Caltag).
In vivo challenge. Four wk after immunization, mice were anesthetized by intraperitoneal injection of ketamine/xylazine, and infected with 5 μl HSV-1 mP inoculated onto each scarified cornea for a dose of 8×105 pfu. To measure virus replication in the corneal epithelium, eyes were swabbed with moistened cotton-tipped swabs at 4 hr and days 1 through 5 post-infection. Swabs for each mouse were placed together in 1 ml PBS and stored frozen until assay. Virus was quantified on Vero cell monolayers by standard plaque assay. After challenge, body weight, signs of disease and survival were monitored on a daily basis. Mice were weighed individually and mean change from initial body weight was calculated daily for each group. Blepharitis scores were assigned in a blinded fashion based on the following scale: 0—no apparent signs of disease; 1—mild swelling and erythema of the eyelid; 2—moderate swelling and crusty exudate; 3—periocular lesions, and depilation; and 4—extensive lesions, and depilation. Mean daily disease score was calculated for each group. Keratitis was assessed at 9 d and 14 d post-challenge using an opthalmoscope and the proportion of eyes with dense opacity (3+) or complete opacity with corneal ulceration (4+) was recorded. Virus replication in neural tissue was analyzed by dissection of trigeminal ganglia and brainstems from a cohort of mice 4 d after challenge. Tissues were stored frozen until use. For virus titer determination, the tissues were thawed and disrupted using a Mini-Bead Beater (BioSpec, Inc.), and then diluted for standard plaque assay.
Assessment of latency by real-time PCR. TG were collected from surviving mice 30 d post-infection and stored at −80° C. DNA was isolated from the TG using a QIAamp DNA Mini Kit (Qiagen) according to the manufacturer's instructions. PCR reactions were run in 25 μl reaction vol using FastStart SYBR Green Master (Rox) (Roche), and primers at 300 nM final concentration. For GAPDH, reactions used 10 ng template DNA and primers forward 5′-GAGTCTACTGGCGTCTTCACC-3′ and reverse 5′-ACCATGAGCCCTTCCACAATGC-3′ which amplify a 337 bp product. For HSV-1 UL50, reactions used 125 ng template DNA and primers forward 5′-CGGGCACGTATGTGCGTTTGTTGTTTAC-3′ and reverse 5′-TTCCTGGGTTCGGCGGTTGAGTC-3′ which amplify a 195 bp product. Reactions were performed using an ABI Prism 7500 real-time PCR system (Applied Biosystems) and cycle conditions: 2 min at 50° C., 10 min at 95° C., 40 cycles of 95° C. for 15 sec and 60° C., and dissociation at 95° C. for 15 sec. Specificity was verified by melting curve analysis. The average of duplicate wells yielded the Ct value, and the UL50 signal for each sample was normalized to the GAPDH signal content by determination of ΔCt. Fold decrease in UL50 content of TG from Δ41Δ29B7-1 and Δ41Δ29B7-2 immunized mice relative to mice receiving Δ41Δ29 was determined using the 2(ΔΔCτ) method (Livak K J and Schmittgen; Pfaffl M W 2001). Fold decrease in UL50 content of TG from immunized mice relative to TG from a mouse receiving control supernatant was determined using the 2(ΔΔCτ) method (Livak K J and Schmittgen; Pfaffl M W 2001). To ensure accuracy of the UL50 level in the two TG from the control supernatant group, the TG were thrice assayed in duplicate and the average ΔCt was calculated from the 6 values obtained.
Statistics. Significance of difference in virus or antibody titers on individual days was determined by Student's t test. Proportion of mice with keratitis or surviving infection was compared using the Fisher exact method. The Kruskal-Wallis non-parametric test was used to assess the significance of difference in blepharitis scores on individual days post-challenge. Relative levels of latent viral DNA between immunization groups were compared by one way analysis of variance (ANOVA) with the Bonferroni post hoc test for multiple groups. Each virus-immunized group was compared with the control supernatant group using the Dunnett t post hoc test. Only one mouse immunized with control supernatant survived challenge; however, a test of homogeneity of variances was not significant.
Results.
In vitro characterization. The murine B7-1 and B7-2 ORFs downstream of the HCMV IEp were inserted into the HSV-1 thymidine kinase (tk) (UL23) ORF in plasmid p101086.7BglII at a unique BglII site engineered 751 bp from the 5′ end of the ORF. The resulting plasmids were cotransfected into S2 cells with full-length DNA from the replication-defective HSV-1 strain Δ41Δ29 which contains a lacZ insertion in the ICP8 ORF and a deletion in the vhs ORF as shown in FIG. 1A. Plaques were isolated from the cotransfection mixture in the presence of acyclovir, and cells infected with the plaque isolates were screened for expression of B7 molecules by flow cytometry and triply plaque-purified. B7-1- and B7-2-expressing recombinants were named Δ41Δ29B7-1 and Δ41Δ29B7-2, respectively. Southern blot analysis was used to verify insertions into the tk ORF in Δ41Δ29B7-1 and Δ41Δ29B7-2 as shown in FIG. 1B. Genomic DNAs purified from the Δ41Δ29 parental and potential recombinant viruses were restricted with EcoRI, electrophoresed, transferred to membrane, and hybridized to a 32P-labeled fragment of p101086.7 DNA. The Southern blot of Δ41Δ29 showed a single fragment of expected size (2.4 kb as shown in FIG. 1B, lane 1), and single fragments of expected sizes, 3.2 kb and 2.9 kb for the B7-1- and B7-2-containing viruses, respectively, as shown in lanes 2 and 3 of FIG. 1B.
Expression of B7 costimulation molecules on the surface of cells infected in vitro with Δ41Δ29B7-1 or Δ41Δ29B7-2 was verified by flow cytometry. S2 cells were mock infected or infected at MOI 5 and collected and stained 24 h later with anti-B7-1 and B7-2 antibodies. Mock-infected cells showed no staining above background, whereas cells infected with Δ41Δ29B7-1 or Δ41Δ29B7-2 stained brightly with B7-1 or B7-2, respectively, as shown in FIGS. 2B and C. Thus, host costimulation molecules expressed from the HSV genome are expressed at uniformly high levels on the infected cell surface.
Immune response to immunization. The capacity of B7 costimulation molecules expressed from the immunizing virus to elicit cellular and humoral immune responses was determined. CD8 T-cells were analyzed because they are thought to play a dominant role in suppressing virus replication in the cornea and trigeminal ganglia. To determine the capacity of the various vaccine prototype strains to elicit HSV-specific CD8 T-cells, mice were immunized s.c. with 4×104 pfu of the parental replication-defective/vhs− virus Δ41Δ29, Δ41Δ29B7-1 or Δ41Δ29B7-2, or an equivalent amount of control supernatant. Six days later cells in the draining lymph nodes were analyzed by IFNγ ELISpot specific for CD8 T-cells recognizing the immunodominant epitope gB498-505 restricted by H-2Kb. Mice immunized with control supernatant showed very low background of IFNγ-producing cells (FIG. 3A). Δ41Δ29, Δ41Δ29B7-1 and Δ41Δ29B7-2 all stimulated strong HSV-specific responses from CD8 T-cells in the draining lymph nodes acutely after immunization, with a slightly greater response from mice immunized with Δ41Δ29B7-2 (FIG. 3B). This observation was corroborated by analysis using tetramer staining of CD8 T-cells specific for the gB498-505 epitope. Some binding of tetramer to CD8+ cells from mice immunized with control supernatant was routinely observed (FIG. 3B and Table 1).







TABLE 1







CD8+, tetramer+ cells in the draining lymph


nodes 6 d after immunization.












% CD8+
Total CD8+


Immunization
Cells recovered
tetramer+ cells
tetramer+ cells





Control supe
1.0 × 107
0.8
1.3 × 104


□41□29
1.2 × 107
2.1
4.2 × 104


B7-1+
1.3 × 107
2.9
5.3 × 104


B7-2+
1.9 × 107
2.6
8.3 × 104









However, staining of cells from mice immunized with Δ41Δ29 was greatly increased over staining of cells from mice receiving control supernatant (FIG. 3B and Table 1). Mice immunized with Δ41Δ29B7-1 or Δ41Δ29B7-2 both contained a slightly higher percentage and absolute number of tetramer+ CD8+ cells in the draining lymph nodes 6 days after immunization than mice immunized with Δ41Δ29 (Table 1). Collectively, these assays suggest slightly stronger stimulation of acute HSV-specific T-cell responses by replication-defective viruses when the viruses express B7-1 or B7-2 costimulation molecules.
A tetramer assay was also performed on cells in the draining lymph nodes of mice 6 d after corneal challenge. Cervical lymph node cells from mice immunized with either B7-1 or B7-2-expressing viruses stained more heavily with the gB498-505 tetramer than cells from □41□29- or control supernatant-immunized mice, and yielded twice as many tetramer-positive cells (Table 2). Thus, vaccinated mice responding to HSV-1 ocular challenge have more HSV-specific T-cells available to fight the infection if exogenous B7-1 or B7-2 was expressed by the immunizing virus.







TABLE 2







CD8+, tetramer+ cells in the draining lymph


nodes 6 d after corneal challenge.












% CD8+
Total CD8+


Immunizationa
Cells recovered
tetramer+ cells
tetramer+ cells





Control supe
1.9 × 108
1.9
3.8 × 106


□41□29
1.0 × 108
2.5
2.5 × 106


B7-1+
1.8 × 108
3.2
5.9 × 106


B7-2+
1.2 × 108
4.6
5.3 × 106





aMice were immunized with 4 × 104 pfu of the indicated virus and challenged 28 d later.






The capacity of the vaccines to elicit HSV-specific antibody was determined by immunizing groups of mice s.c. with 4×105 pfu (high), 4×104 pfu or 4×103 pfu (low) doses of the parental ICP8−/vhs− virus Δ41Δ29B7-1 or Δ41Δ29B7-2. Three weeks after immunization, blood was collected and HSV-specific IgG titers in the serum were determined by ELISA. Antibody titers elicited by Δ41Δ29B7-1 or Δ41Δ29B7-2 were not significantly greater than that induced by Δ41Δ29 at all three immunizing doses (FIG. 4). Thus, Δ41 Δ29B7-1 and Δ41Δ29B7-2 induced somewhat stronger HSV-specific T-cell responses by this did not manifest as additional help for antibody production.
Protective effect of the vaccines. At 4 wk post-challenge, mice were challenged on the cornea with the virulent HSV-1 strain mP. Replication in the corneal epithelium was quantified over the first 4 days post-challenge by titer of virus collected on corneal swabs. Mice immunized with control supernatant sustained high levels of challenge virus replication in the corneal epithelium (FIG. 5). The Δ41Δ29 parental virus had no effect on challenge virus replication at day 1 or day 2 post-challenge at any immunizing dose. In contrast, by day 2, immunization with the high dose of B7-1 or B7-2-expressing viruses was able to reduce challenge virus replication 200-fold compared to control-immunized mice, and 50-fold compared Δ41Δ29 (FIG. 5A). Immunization of mice with the high dose of all three replication-defective viruses helped mice nearly resolve HSV-1 replication in the cornea by day 4 post-challenge (FIG. 5A). At the medium immunizing dose, the three replication-defective viruses significantly reduced challenge virus replication by 3 days post-challenge and even more so by 4 days post-challenge (FIG. 5B). However, neither B7-expressing virus improved protection over what was observed with Δ41Δ29. At the lowest immunizing dose, all replication-defective viruses equivalently reduced challenge virus replication in the corneal epithelium, but only at 4 days post-challenge (FIG. 5C). Thus, addition of B7-1 or B7-2 to an ICP8−vhs− vaccine strain has a transient but significant impact on protection of the corneal epithelium when given at the high (4×105 pfu) immunizing dose.
Blepharitis developed in mice immunized with control supernatant by 4 days post-challenge, and became severe by 7 d post-challenge (FIG. 6). In marked contrast, all 3 replication-defective vaccine strains protected mice almost completely from developing any inflammation of the eyelid (FIG. 6A). When given at the medium dose, Δ41Δ29 protected mice from severe blepharitis, but moderate inflammation was observed from 4 through at least 9 days post-challenge (FIG. 6B). In contrast, in mice immunized with Δ41Δ29B7-1 and Δ41Δ29B7-2, blepharitis was mild and decreased from 4 through 9 d post-challenge (FIG. 6B), though the difference with Δ41Δ29 did not achieve statistical significance. The lowest immunizing dose did not afford protection from blepharitis to any of the mice (FIG. 6C), although fewer eyelids of those immunized with any of the viruses showed periocular lesions. Thus, vaccine strains encoding either B7-1 or B7-2-slightly enhanced protection from blepharitis over that afforded by Δ41Δ29 after HSV-1 ocular infection.
Keratitis was assessed in all surviving mice at 9 and 14 d post-challenge. Δ41Δ29 given at the high dose protected mice almost completely from developing severe (sight-impairing) keratitis, and no mouse immunized with the high dose of Δ41Δ29B7-2 showed severe corneal disease (FIG. 7A). At the medium immunizing dose, 80% of mice receiving control supe had sight-compromising corneal disease (FIG. 7B). Mice immunized with Δ41Δ29 showed slightly less disease incidence, but 60% of corneas still were severely affected. In contrast, prior immunization of mice with the medium dose of either B7-1 or B7-2-expressing virus very significantly protected them from developing severe corneal disease after HSV-1 infection (FIG. 7B). When given at the low dose, all three vaccine strains only slightly reduced the incidence of severe keratitis compared with control supernatant (FIG. 7C). Thus, immunizations using the medium dose (4×104 pfu) revealed a significant capacity of B7 molecules, encoded by the vaccine virus, to enhance protection from development of keratitis afforded by replication-defective/vhs-virus.
The HSV-1 mP strain causes a virulent infection when inoculated by the corneal route. Ocular challenge with mP resulted in the death of most mice immunized with control supernatant by 9 d post-challenge (FIG. 8). Prior immunization with the ICP8−/vhs− parental virus or either strain encoding B7 molecules completely protected mice from lethal infection when given at the high or medium doses (data not shown). Even when as few as 4000 pfu of supernatant-derived vaccine virus (low dose) was used, at least half of the mice were subsequently protected from lethal infection (FIG. 8). The B7-1-expressing virus prolonged survival compared with parental or B7-2-expressing vaccine strains, but ultimately no significant difference between the three vaccine strains in capacity to protect against mortality was observed.
We also determined whether the B7-1 or B7-2 vaccine viruses enhanced protection of the nervous system compared with the ICP8−vhs− parental virus. Mice immunized with medium dose of vaccine were chosen for analysis because this dose had permitted the best distinction between immunizing strains based on parameters of disease. To assess vaccine capacity to reduce establishment of latent infection, TG were removed from mice 1 month after challenge and their burden of latent challenge virus genome was assessed. DNA was prepared from individual TG and subjected to real-time PCR using primers for UL50 to detect viral genomes and for GAPDH as a normalization control (FIG. 9). B7-1 and B7-2 afforded slightly better protection from latent infection of the nervous system by challenge virus than Δ41Δ29 (FIG. 9A), although the difference was not statistically significant. All three vaccine strains reduced latent infection of the TG by challenge virus to 8-fold when compared with the genome load in TG of a mouse immunized with control supernatant that survived challenge (FIG. 9B). This result may underestimate the difference between virus-immunized and control animals because the vast majority of mice immunized with control supernatant are so extensively infected that they do not survive challenge.
To determine whether protection from keratitis by Δ41Δ29B7-1 and Δ41Δ29B7-2 viruses was related to the level of challenge virus reaching the nervous system acutely after challenge, TG and brainstems were isolated from immunized mice sacrificed 3 d post-challenge and virus titer in the tissues was determined All vaccine strains protected the nervous system better than control supernatant (FIG. 10). B7-1 and B7-2 showed a trend toward better protection against acute infection of the nervous system by the challenge virus than Δ41Δ29, although only B7-2 improved protection to a statistically significant degree.
Having described the invention in detail, those skilled in the art will appreciate that modifications may be made of the invention without departing from the spirit and scope thereof. Therefore, it is not intended that the scope of the invention be limited to the specific embodiments described. Rather, it is intended that the appended claims and their equivalents determine the scope of the invention.

## Claims

1. A pharmaceutical composition for use in enhancing an immune response to herpes simplex virus (HSV) comprising an effective amount of a B7 costimulation molecule-expressing, replication-defective ICP8− HSV and a carrier, wherein after administration of said pharmaceutical composition to a vertebrate, said costimulation molecule is expressed on the surface of infected cells.

2. The pharmaceutical composition of claim 1, wherein said HSV is virion host shutoff protein deficient.

3. The pharmaceutical composition of claim 1, wherein said HSV is a ICP8−vhs−B7+ HSV-1 or -2 replication-defective virus.

4. A method of enhancing an immune response to herpes simplex virus (HSV) in a vertebrate comprising the steps of: administering an effective amount of a B7 costimulation molecule-expressing replication-defective HSV and a carrier prior to HSV infection, wherein after administration of said pharmaceutical composition to said vertebrate, said costimulation molecule is expressed on the surface of infected cells.

5. The method of claim 4, wherein said HSV is an ICP8− defective virus.

6. The method of claim 4, wherein said HSV is virion host shutoff protein deficient.

7. The method of claim 4, wherein said HSV is a ICP8−vhs−B7+ HSV-1 or -2 replication-defective virus.

8. A method of manufacturing a pharmaceutical composition for enhancing an immune response to herpes simplex virus comprising the steps of: combining an effective amount of a B7 costimulation molecule-expressing ICP8− replication-defective herpes simplex virus and a carrier, wherein said virus expresses said costimulation molecule on the surface of infected cells after administration of said pharmaceutical composition to a vertebrate.

9. The method of claim 8, wherein said HSV is virion host shutoff protein deficient.

10. The method of claim 8, wherein said virus is an ICP8−vhs−B7+ HSV-1 or -2 replication-defective virus.