HoneywellPerformanceMaterialsandTechnologiesBuffaloResearchLaboratory20PeabodyStreetBuffalo,NY14210716-827-6200ManagerofStandardsASHRAE1791TullieCircleNEAtlanta,GA30329-2305HoneywellDecember12,2013Re:ASHRAEStandard34RefrigerantDesignationandSafetyClassificationApplicationforR-134a/R-1234ze(E)(42.0/58.0%bymass)DearSirs;PleasefindherewithsupportingdataandrequiredcertificationsforHoneywell'sapplicationtoASHRAESSPC34forassignmentofanumberdesignationandsafetyclassificationforR-134a/R-1234ze(E)(42.0/58.0%bymass).WerequestthisapplicationforsubmissionandconsiderationatthescheduledJanuary,2014meetinginNewYork,NewYork.Enclosedare2boundcopiesofourapplicationforadministrativeuse.Anelectronicversionwillbee-mailedseparately.Sincerelyyours,MarkW.SpatzGlobalLeaderRefrigerantTechnology-HoneywellRefrigerantsHONEYWELLINTERNATIONALInc.SubmissiontoASHRAESSPC34CommitteeDecember12,2013RequestforRefrigerantDesignationandSafetyClassificationForR-134a/R-1234ze(E)(42.0/58.0%bymass)PRIMARYCONTACTMr.MarkW.SpatzGlobalLeaderRefrigerantTechnologyHoneywellFluorineProducts-1-TABLEOFCONTENTSCover1Contents2Section1-AdministrativeInformation3Section2-DesignationInformation7Section3-ToxicityData10Section4-FlammabilityInformation29Section5-RCLValueIContaminantsandImpurities53Section6 -Appendices55.2.HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Section1:AdministrativeInformation-3-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013IApplicantIdentification(ANSI/ASHRAEStandard34-2010Section9.4.1)APPLICANTAddress:PrimaryContact:Address:Telephone:FAX:Email:OtherContacts:Address:Telephone:FAX:Email:HoneywellInternationalInc.SpecialtyMaterials101ColumbiaRoadMorristown,NJ07962Mr.MarkW.SpatzGlobalLeaderRefrigerantTechnologyHoneywellFluorineProductsBuffaloResearchLaboratory20PeabodyStreetBuffalo,NY14210(716)827-6238(716)827-6275mark.spatz@honeywell.comGeorgeKoutsaftesBusinessDirectorHoneywellSpecialtyMaterials-Refrigerants101ColumbiaRoadMorristown,NJ07962(973)455-2996(973)455-6394george.koutsaftes@honeywell.comApplicant'sInterest:HoneywellFluorineProductsDivisiondesirestomanufactureandsellthesubjectblendasarefrigerantforrefrigerationandair-conditioningapplications.Honeywell Application

to ANSIIASHRAE

Standard

34 R-134a/R-1234ze(E)

(42.0/58.0%

by mass)

December

2013 I Honeywell

Data Certification (ANSI/ASHRAE

Standard

34-2010

Section

9.4.2) We certify

that

the information

provided

in this application

(including

its appendices)

is true and accurate

to the best

of my/our knowledge

and

that

no information

that would

affect classification

of toxicity

or flammability

safety

is being withheld.

1/We further

certify

that

we have reviewed ANSI/ASH

RAE Standard

34-2010 (including

all published

addenda

thereto) and

that

the information

provided

in this application

is consistent

with

the requirements

of that standard. **~~**

**4** Mark

W. Spatz

George Koutsaftes Global

Leader Refrigerant Technology

Business

Director Honeywell

Specialty

Materials

Honeywell

Specialty

Materials

- Refrigerants Primary

Contact

Alternate

Contact -5-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013HoneywellDesignationandClassificationCertification(ANSI/ASHRAEStandard34-2010Section9.4.3)WeunderstandthatdesignationsandsafetyclassificationsrecommendedforpublicreviewapprovalorpublicationarenotassignedandmayberevisedordisapprovedUntilactuallypublishedinanaddendumorrevisiontoStandard34.MarkW.SpatzGeorgeKoutsaftesGlobalLeaderRefrigerantTechnologyBusinessDirectorHoneywellSpecialtyMaterialsHoneywellSpecialtyMaterials-RefrigerantsPrimaryContactAlternateContact•6-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Section2:DesignationInformation-7-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)IRefrigerantData(ASNI/ASHRAEStandard34-2010Section9.5DesignationInformation-Section9.5ApplicationDecember2013HoneywellFluorineProductsDivision,manufacturer,submitsthisapplicationtoASHRAEunderANSI/ASHRAEStandard34-2010(includingpublishedaddenda)forassignmentofaRefrigerantDesignationandSafetyClassificationforourzeotropicrefrigerantblendofR-134a/R-1234ze(E)(42.0/58.0%bymass),asdefinedunderDesignationInformationofthisapplication.ThisrefrigerantblendhasbeendevelopedasalowerglobalwarmingreplacementforRefrigerantR-134a.RefrigerantIdentification-Section9.5.1Therefrigerantblendisformulatedwiththefollowingcomponents(inorderofincreasingboilingpoints):R-134af/R-1234ze(E)(42.0/58.0%bymass)(+/-2.0/±2.0%bymass)Therefrigerantcomponentsofthezeotropicblendasdefinedabovearedescribedas:ChemicalNameChemicalFormulaCASNo.R-134a1,1,1,2-TetrafluoroethaneR-1234ze(E)Trans-1,3,3,3-Tetrafluoro-1-propeneCHFCHCF329118-24-9tPropertiesofIndividualComponents-ASHRAESandard34-2010Section9.5.2.1MolecularMass,g/moleNormalBoilingPointat101.3kPa,ocSaturatedVaporPressureat20°C,kPaSaturatedVaporPressureat60°C,kPaTemperatureattheCriticalPoint,ocPressureattheCriticalPoint,kPaSpecificVolumeattheCriticalPoint,m3/kgUsesandTypicalApplicationTemperatures:Evaporatingtemperaturesintherangebutnotnecessarilylimitedtothatrange.Condensingtemperaturesintherangebutnotnecessarilylimitedtothatrange.R-134a102.0-26.15721682101.140590.00195Domesticandcommercialrefrigeration;asablendcomponentofR-407C,R-404A,andthelike.-8-R-1234ze(E)114.0-19.04271277109.436360.00204Airconditioningandrefrigeration;asablendcomponent.HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)ZeotropicBlend-Section9.5.2.3FormulationMolecularMass(asformulated)MolecularMassofVaporat60°CBubblePointat101kPaDewPointat101kPaMaximumTemperatureGlideAtNormalBoilingPointAt20°CLatentHeatofVaporizationat60°CSpecificHeatRatioofVaporat60°CTemperatureatCriticalPointPressureattheCriticalPoint,kPaSpecificVolumeattheCriticalPointMolebasisandMassbasisUsesThesubjectrefrigerantblendisproposedforapplicationshistoricallyservedbyR-134ainvaporcompressionsystems.TypicalApplicationTemperaturesEvaporatingtemperaturesintherangeof-40°Cto+1oocbutnotnecessarilylimitedtothatrange.Condensingtemperaturesintherangeof+15°Cto+65°Cbutnotnecessarilylimitedtothatrange.ProposedCompositionTolerancesforClassificationR-134a/R-1234ze(E)(42.0/58.0%bymass)108.7g/mole108.1g/mole-23.4°C-22.8°C0.6°C0.6°C135.2kJ/kg1.36104.4°C3819R-134a(1,1,1,2-Tetrafluoroethane)±2.0%bymassNominal42.0%bymassR-1234ze(E)(trans-1,3,3,3-±2.0%bymassTetrafluoroprop-1-ene)Nominal58.0%bymassDecember2013WorstCaseFormulation(WCF)R-134a/R-1234ze(E)(40.0/60.0%bymass)WorstCaseFractionationFormulationFinalliquidcomposition,-13.2C,vaporleakfromacylinderat90%ofmaximumallowedDOTfill.R-134a/R-1234ze(E)(17.3/82.7%bymass)RefrigerantBlendCriticalTemperatureandCriticalPressure-ASHRAEStandard34Section9.5.2.5CriticalTemperature:104.4°CCriticalPressure:3819kPaCriticalSpecificVolume:0.00203m3/kg-9-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Section3:ToxicityInformation-10-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)IRefrigerantData(ASNI/ASHRAEStandard34-2010Section9.6ToxicityInformation-ASHRAEStandard34-2010Section9.6ToxicityinformationforHFC-134aandHF0-1234ze(E)areprovidedinthissection.ToxicityInformationHFC-134aAcuteToxicitya.ACGIHTLV-c:Noneassignedb.ACGIHTLV-STEL:Noneassignedc.NIOSHIDLH:Noneassignedd.4-hrALCrat=>359,300ppm(v/v)<5>e.LD50:>359,300ppm<5>December2013f.Cardiacsensitization:TheNoObservedEffectLevelforepinephrinechallengeddogsis50,000ppm(v/v)andtheLowestObservedEffectLevelis75,000ppm(v/v).(7)g.Anestheticandcentralnervoussystemeffects:EC50270,000ppm.<6>h.Otherescape-impairingeffectsandpermanentinjury:Nonereported.ANIMALTOXICITYDATAA.AcuteToxicityandIrritancyA.AcuteToxicityandIrritancy1.OralSubstanceisagasandhasnotbeentestedfororaltoxicity.2.EyeIrritationSubstanceisagasandinaspecializedstudyforeyeirritationwhereitwassprayedintotheeyeofarabbitfor5or15seconds,itwasfoundtobeveryslightlyirritating.Itisnotpossibletodetermineiftheirritationwasfrompotentialfreezingassociatedwiththeevaporationofthetestsubstanceorsomeotherpropertyofthesubstance.However,ina4-weekanda13weekinhalationtoxicitystudywithexposuresupto5%inair,6-hours/day,5days/week,nosignsofirritationwereseen.1113.SkinAbsorptionNodata4.SkinIrritationSubstanceisagasandinaspecializedstudyforskinirritationwhere0.5mlwasappliedtoasquareof8gauzepads,itwasfoundtobeveryslightlyirritating.Itisnotpossibletodetermine-11-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013iftheirritationwasfrompotentialfreezingassociatedwiththeevaporationofthetestsubstanceorsomeotherpropertyofthesubstance.Typically,exposuretorefrigerantgasescanleadtocoolingoftheskinandfrostbite.1215.SkinSensitizationFollowingasingleinjectionofFreund'scompleteadjuvant,guineapigsweretreatedwith7applicationsof0.5mlofHFC-134aappliedtogauzepadsontheirbacks.Treatmentswereseparatedby2-3days.Afteraperiodof12days,theguineapigswerechallengedwithan8thapplicationof0.5mlofHFC-134a.Therewasnoevidenceofsensitization.1316.InhalationToxicitya.Anapproximatelethalconcentrationfora4-hourexposure,thelowestconcentrationthatproduceddeath,was567,000ppm.Inthisstudy,mortalitieswere0/6at81,100,205,200and359,300ppm,5/6at566,700ppm,0/6at646,700ppmand2/6at652,700ppm.Therangeofconcentrationsinthe566,700exposurelevelgroupwas494,400to668,800ppmandthusoverlappedwiththehigherexposures.Mostratsshowedsignsofdecreasedactivityanddyspnea,salivationandlachrymationduringtheHFC-134aexposure.Sincethehigherexposurelevelsoverlapped,thebestestimatefortheLC50wastakenas>359,300ppm.141b.Inanundocumentedstudy,Collinsreportedthatthe4-hrLC50forexposuretoHFC-134awas>500,000ppmandthe15minuteLC50was>800,000ppm.151c.HFC-134awasnotlethaltodogsexposedtoalevelof700,000or800,000ppmfor3to5hours.151d.A10minuteECsoforanestheticeffects,measuredbylossoftherightingreflex,was280,000ppmintheratand270,000ppminthemouse.161e.Inthisstudyagroupof8beagledogswereexposedtolevelsof50,000,75,000and100,000ppmofHFC-134a.Initiallyadeterminationwasmadeforthemaximumlevelofepinephrinethatwouldnotcauseacardiacarrhythmia.Thedogswerethenexposedtothetestcompoundforatotalof10minutes.Afterthefirstfiveminutesofexposure,eachdogreceivedaninjectionofepinephrineatthepre-determinedmaximumsub-arrhythmiadose.Duringthenextfiveminutesofexposure,thedogsweremonitoredforthedevelopmentofacardiacarrhythmia.Therewerenoarrhythmias,norothersignsoftoxicityseeninthedogsexposedto50,000ppm.Exposureofdogstolevelsof75,000ppmand100,000ppmofHFC134aresultedinarrhythmias.Itwasconcludedthatexposureto75,000ppmrepresentedthethresholdforcardiacsensitization.(7)-12.HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013f.Inasecondstudyagroup10ofbeagledogswereexposedtolevelsof40,000,80,000,160,000,and320,000ppmofHFC-134a.Thedogswereexposedtothetestcompoundforatotalof10minutes.Afterthefirstfiveminutesofexposure,eachdogreceivedaninjectionof8JJg/kgofepinephrine.Duringthenextfiveminutesofexposure,thedogsweremonitoredforthedevelopmentofacardiacarrhythmia.Therewerenoarrhythmias,norothersignsoftoxicityseeninthedogsexposedto40,000ppm.Exposureofdogstolevelsof80,000ppmandaboveofHFC-134aresultedinarrhythmias.Itwasconcludedthatexposureto80,000ppmrepresentedthethresholdforcardiacsensitization.181B.RepeatExposureToxicityDataa.SubacuteToxicityInthisGLPstudygroupsof16maleand16femaleAlpk/APfSDWistar-derivedratswereexposed,wholebody,tovaporsofHFC-134aatlevelsof0(control),1,000,10,000or50,000ppm6-hours/day,5days/weekfor4weeks.Therewerenoobservationsofclinicalabnormalities,noradverseeffectsonbodyweightgain,foodconsumption,nortreatmentrelatedchangesinWBCparameters.Therewerenoadversefindingsintheanalysisoforganweights,ormacroscopicormicroscopicexaminationoftissues,includingacompleteexaminationoftissuesfromtherespiratorytractOverall,itcanbeconcludedthattheNo-Adverse-Effect-Level(NOAEL)fora4-weekexposuretoHFC-134awasatleast50,000ppm.191b.SubchronicToxicityInthisGLPstudygroupsof20maleand20femaleAlpk/APfSDWistar-derivedratswereexposed,wholebody,tovaporsofHFC-134aatlevelsof0(control),2,000,10,000or50,000ppm6-hours/day,5days/weekfor13weeks.Therewerenoobservationsofclinicalabnormalities,oradverseeffectsonbodyweightgain,foodconsumption,nortreatmentrelatedchangesinWBCparameters.Therewerenoadversefindingsintheanalysisoforganweights,ormacroscopicormicroscopicexaminationoftissues,includingacompleteexaminationoftissuesfromtherespiratorytract.Overall,itcanbeconcludedthattheNo-Adverse-Effect-Level(NOAEL)fora13-weekexposuretoHFC-134awasatleast50,000ppm.1101c.ChronicToxicity/CarcinogenicityInthisGLPstudygroupsof85maleand85femaleAlpk/APfSDWistar-derivedratswereexposed,wholebody,tovaporsofHFC-134aatlevelsof0(control),2,500,10,000or50,000ppm6-hours/day,5days/weekfor104weeks.Therewerenoobservationsofclinicalabnormalities,-13-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013noradverseeffectsonbodyweightgain,foodconsumption,nortreatmentrelatedchangesinWBCparameters.Theonlytreatmentrelatedeffectoftoxicologicalsignificancewasinthetestesofthe50,000ppmexposedmalerats.TherewasastatisticallysignificantincreaseintheweightofthetesteswhencomparedtothecontrolandtherewasanincreasedincidenceofLeydigcellhyperplasiaandbenignLeydigcelltumors.Overall,itcanbeconcludedthattheNoObserved-Adverse-Effect-Level(NOAEL)forthechronicstudywithHFC-134awas10,000ppm.1111d.Reproductive/DevelopmentalToxicityInthisGLPstudygroupsof29-30pregnantAlpk/APfSDWistar-derivedratswereexposed,wholebodytovaporsofHFC-134aatlevelsof0(control),1,000,10,000or50,000ppm6-hours/day,7days/weekduringdays6-15ofgestation.Therewerenodifferencesintheincidenceofthenumberofimplantationsites,pre-andpostimplantationlosses,liveanddeadfetuses,resorptions,orratioofmalestofemalesbetweenthegroups.Skeletalossificationwasslightlydelayedat50,000ppm.ItwasconcludedthatHFC-134aisneitherteratogenicnorembryotoxicatexposurelevelsupto50,000ppm,butatthehighestlevelHFC-134amaybeslightlyfetotoxic.TheclearNOELforthisstudywas10,000ppm.'12'AGLPstudywasconductedusinggroupsof28pregnantNewZealandwhiterabbitsexposedtolevelsof0(control),2,500,10,000and40,000ppm.Againtheexposureswere6-hrs/day,fromdays7-19ofgestation.Theexposuresat10,000and40,000ppmresultedinslightlyretardedbodyweightgainsanddecreasedfoodconsumption.Nosignificanteffectswereseeninthepupsfromanyexposuregroup.Itwasconcludedthattheno-observed-effect-levelformaternaltoxicitywasatleast2,500ppmandforthepupsitwas40,000ppm.1131InaGLPstudyconductedtosupportthedevelopmentofHFC-134aforuseinmedicalinhalers,HFA-134awasadministeredtoAHAratsbysnout-onlyinhalationexposurefor1hour/dayatlevelsupto50,000ppm,throughoutgametogenesis(sexualdevelopment),mating,pregnancyandlactation.Theonlytreatmentrelatedeffectwasaslightreductioninbodyweightgainseeninthemaleratsinthe50,000ppmexposurelevelgroup.ExposuretoHFA-134adidnotcauseandeffectsinreproductiveperformance,developmentofmaturationduringtwosuccessivegenerations.1141e.MutagenicityAGLPAmesassaywasconductedwithHFC-134awhichinvolvedexposureofbacterialcellsTA1535,TA1537,TA98,TA100,andWP2uvrAbothwithandwithoutS-9metabolicactivation.-14-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013Exposurelevelsofupto100%%wereused.Undertheconditionsofthistest,HFC-134awasnotactive.1151InthesecondGLPstudy,culturedhumanlymphocyteswereexposedtolevelsofHFC-134aofupto75%bothwithandwithoutS-9metabolicactivation.Undertheconditionsofthistest,HFC-134awasnotactive(notclastogenic)inthisassay.1161IntheGLPmicronucleusstudy,NMRImicereceivedasingleexposurebyinhalationto50,000,150,000and500,000ppm.Bonemarrowcellswerecollectedandanalyzedforthepresenceofmicronuclei.UndertheconditionsofthisassayHFC-134adidnotproducechromosomaldamageordamagetothemitoticspindleapparatusinbonemarrowcells.1171TheclastogenicpotentialofHFC-134awasinvestigatedusingaChinesehamsterlungcellline.ThisGLPstudyusedexposuresupto100%for24and48hourswithoutmetabolicactivationorfor6hourswithmetabolicactivation.Whencomparedtothenegativecontrol,therewasnoincreaseintheincidenceofchromosomalaberrations.1181RECOMMENDEDWEELGUIDEAWEELOELhasbeenapprovedbytheWEELCommitteeforHFC-134a.Itrecommends1,000ppmasan8-hourTWA.1191MATERIALSAFETYDATASHEETAHoneywellMaterialSafetyDatasheetforGenetron®134aisattached.1201RECOMMENDEDATELforHFC-134aLC50>359,000PPMCardiacsensitizationNOEL=50,000ppmAnesthetic/CNSeffectEC50=270,000ppmATEL:50,000ppmREFERENCES(1)Mercier0.1990a.HFA-134a:Testtoevaluatetheocularirritationintherabbit.Unpublishedreport912349.Hazleton,Lyon,France.Atochem,Paris,France.(2)Mercier0.1989.HFA-134a:Testtodeterminetheindexofprimarycutaneousirritationintherabbit.Unpublishedreport911422.Hazleton,Lyon,France.Atochem,Paris,France.-15-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013(3)Mercier0.1990b.HFA-134a:Testtoevaluatethesensitizingpotentialbytopicalapplicationsintheguineapig.Theepicutaneousmaximizationtest.Unpublishedreport001380.Hazleton,Lyon,France.Atochem,Paris,France.(4)SilberLS.1979a.AcuteInhalationtoxicitystudyoftetrafluoroethane.UnpublishedreportHLR422-79.HaskelllaboratoryforToxicologyandIndustrialMedicine.DuPontdeNemours,Newark,DE,USA.(5)CollinsMA.1990.Klea134a:Acuteinhalationdata.Personalcommunication.ICIChemicals&Polymers,Runcorn,Cheshire,UK.(6)ShulmanM,SadoveMS.1967.1,1,1,2-Tetrafluoroethane:Aninhalationanestheticagentofintermediatepotency.AnesthAnatgCurrentResearches46:629-633.(7)MullinLS.1979.FC134a:Cardiacsensitization.Unpublishedreport42-79.HaskellLaboratoryforToxicologyandIndustrialmedicine.DuPontdeNemours,Newark,DE,USA.(8)HardyCJ,SharmanIJ,ClarkGC.1991.Assessmentofcardiacsensitizationindogs:ComparisonofHFA134aandA12.UnpublishedreportCTUC/2521.ICICentralToxicologyLaboratory,AlderleyPark,Macclesfield,Cheshire,UK.(9)RileyRA,BennettIP,ChartIS,GoreCW,RobinsonM,WeightTM.1979.Arcton134a:Subacutetoxicitytotheratbyinhalation.UnpublishedreportCTUP/463.ICICentralToxicologyLaboratory,AlderleyPark,Macclesfield,Cheshire,UK.(10)HextPM.1989.90-Dayinhalationtoxicitystudyintherat.UnpublishedreportCTUP/2466.1CI,CentralToxicologylaboratory,AlderleyPark,Macclesfield,Cheshire,UK.(11)HextPM,Parr-DobrzanskiRJ.1993.HFC-134a:2-Yearinhalationtoxicitystudyintherat.UnpublishedreportCTUP/3841.Zeneca,CentralToxicologyLaboratory,AlderleyPark,Macclesfield,Cheshire,UK.(12)HodgeMCE,KilmartinM,RileyRA,WeightTM,WilsonJ.1980.Arcton134a:Teratogenicitystudyintherat.ICI,CentralToxicologyLaboratory,AlderleyPark,Macclesfield,Cheshire,UK.-16-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0o/obymass)December2013(13)CollinsMA,RuschGM,SatoF,HextPM,MillischerJR.1995.1,1,1,2-Tetrafluoroethane:Repeatexposureinhalationtoxicityintherat,developmentaltoxicityintherabbit,andgenotoxicityinvitroandinvivo.FundAppltoxico/25:271-280.(14)Alexander,OJ,Libretto,SE,Adams,MJ,Hughes,EW,andBannerman,M.HFA-134a(1,1,1,2-tetrafluoroethane):effectsofinhalationexposureuponreproductiveperformance,developmentandmaturationofrats.HumanandExperimentalToxicol.15,508-517(1996).(15)ArakiA.1991.Reportonreversemutationassayinbacteriaontetrafluoroethane.Unpublishedreportstudy5292,5312.JapanBioassayLaboratory,JapanIndustrialSafetyandHealthAssociation,Hirasawa,Hadano-shi,Japan.(16)MackayJM.1990.HFC-134a:Anevaluationintheinvitrocytogeneticassayinhumanlymphocytes.UnpublishedreportCTUP/2977.ICI,CentralToxicologyLaboratory,AlderleyPark,Macclesfield,Cheshire,UK.(17)MullerW,HofmannT.1989.CFC134a:MicronucleustestinmaleandfemaleNMRImiceafterInhalation.Unpublishedreportstudy88.1244.PharmaResearchToxicologyandpathology.Hoechst,Frankfurt,Germany.(18)AskuraM.1991.Reportonachromosomalaberrationtestof1,1,1,2-tetrafluoroethaneinculturedmammaliancells.Unpublishedreportstudy5879.JapanBioassayLaboratory,JapanIndustrialSafetyandHealthAssociation,Hirasawa,Hadano-shi,Japan.(19)AIHA.1991.1,1,1,2-Tetrafluoroethane.Workplaceenvironmentalexposurelevelguide.AmericanIndustrialHygieneAssociation,Fairfax,VA,USA.(20)Honeywell(December6,2012).Genetron®134aMaterialSafetyDataSheet,HoneywellInternational,Inc.,101ColumbiaRoad,Morristown,NJ,USA.-17-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)ToxicityInformationHF0-1234ze(E)AcuteToxicitya.ACGIHTLVifassigned:Noneb.ACGIHTLV-STELifassigned:NeitherACGIHnorAIHArecommendceilingorshorttermexposurelimitsforHF0-1234ze(E).c.NIOSHIDLHifassigned:NIOSHhasnotassignedanIDLHforHF0-1234ze(E).d.4-hrLC50rat>207,000ppm(v/v)15>e.LD50ifavailable:Notapplicabletogasesf.CardiacsensitizationNOEL:>120,000ppm(v/v)16>g.Anestheticandcentralnervoussystemeffects:NOAEL>207,000ppm(v/v).15>RatsthatwereexposedtovaporsofHF0-1234ze(E)atlevelsupto207,000ppmfor4hoursshowednoevidenceofanestheticorCNSeffects15>.h.Otherescape-impairingeffects:Nonehavebeenidentified.ANIMALTOXICITYOATAA.AcuteToxicityandIrritancy1.OralSubstanceisagasandhasnotbeentestedfororaltoxicity.2.EyeIrritationThesubstanceisagasandhasnotbeentestedforeyeirritation.However,in2-week,4-weekand13-weekinhalationtoxicitystudieswithexposuresupto5%,1.5%and1.5%inair,respectively,6-hours/day,5days/week,nosignsofocularirritationwereseen:11•2•3>3.SkinAbsorptionSubstanceisagasandhasnotbeentestedforskinabsorptiontoxicity.4.SkinIrritationDecember2013ThedermalirritationofHF0-1234ze(E)wasevaluatedinaGLPstudyinalbinorabbitsfollowingOECDguideline404:AcuteDermalIrritation/Corrosion.Initially,0.4mloftestsubstancewasappliedtothebackofonerabbitviaahilltopchamberfora 3minuteexposure.Noirritationorfrostbitewasobservedimmediatelyor60minutesfollowingexposure.Next,HF0-1234ze(E)wasappliedtothebackof3rabbitsinasimilarmannerfora 4hourexposure.Noirritationorfrostbitewasobserved60minutes,24,48,or72hoursfollowingexposure.HF0-1234zeisnotadermalirritantinrabbitsundertheconditionsofthisstudy.141-18-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)5.SkinSensitizationSubstanceisagasandhasnotbeentestedforskinsensitization.6.InhalationToxicitya.AGLPacute4-hourinhalationtoxicitystudywasconductedwiththreegroupsof5maleand5femaleSprague-DawleyCDratsthatwereexposednoseonlytovaporsofHF0-1234zeatlevelsof0(control),100,000or207,000ppmfor4hours.Theanimalswereheldfora14-dayobservationperiod.Nomortality,noclinicalsignsoftoxicity,notreatment-relatedchangestobodyweight,andnofoodconsumptionchangeswereobserved.Attermination,grossnecropsyobservationswerenormalandtherewerenotreatment-relatedorstatisticallysignificantdifferencesinabsoluteorrelativeorganweights(kidneys,liverandlungs).The4-hourLC50forHF0-1234ze(E)isgreaterthan207,000ppm.Basedontheseresults,HF0-1234ze(E)isconsideredpracticallynontoxicbytheinhalationrouteofexposure.151b.Inanacutecardiacsensitizationstudy,agroupof6beagledogswereexposedtovaporsofHF0-1234ze(E)atconcentrationsof2%,6%or12%(20,000,60,000or120,000ppm).Exposureswereconductedondifferentdays;withatleasta2-dayseparation.Initiallyadeterminationwasmadeforthemaximumlevelofepinephrinethatwouldnotcauseacardiacarrhythmia.Thedogswerethenexposedtothetestsubstanceforatotalof10minutes.Afterthefirstfiveminutesofexposure,eachdogreceivedaninjectionofepinephrineatthepre-determinedmaximumsub-arrhythmiadose.Duringthenextfiveminutesofexposure,thedogsweremonitoredforthedevelopmentofacardiacarrhythmia.Noarrhythmiasorothersignsoftoxicitywereinducedinanyofthedogs.Thus,itwasconcludedthatexposureofdogstoalevelof12%(120,000ppm)ofHF0-1234ze(E)didnotcausecardiacsensitizationwhenchallengedwithepinephrine.1617.AnestheticandCentralNervousSystemeffects:IntheGLPacute4-hourinhalationtoxicitystudydescribedabove,noneoftheSprague-DawleyCDratsthatwereexposedtovaporsofHF0-1234ze(E)atlevelsupto207,000ppmfor4hoursshowedanyevidenceofanestheticorCNSeffects.151-19-December2013HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)8.Otherescapeimpairingeffects:Nonehavebeenidentified.9.6.2.ChronicToxicityDataB.RepeatExposureAnimalToxicityStudies1)SubacuteToxicityDecember2013a)AGLP2-weekinhalationtoxicitystudywasconductedwithfourgroupsof5maleand5femaleSpragueDawleyratsthatwereexposednoseonlytovaporsofHF0-1234ze(E)atlevelsof0(control),5,000,20,000or50,000ppmfor6-hours/day,5days/weekduringa 2weekperiod,withatotalof10exposuredays.Therewerenotreatment-relatedchanges inclinicalobservations,bodyweightgain,foodconsumptionorfoodconversionefficiency.Hematologicanalysis,clinicalchemistryanalysis,organweightmeasurements,andmacroscopicandmicroscopicexaminationoftheheart,liverandnasalpassagesrevealedtreatmentrelatedeffectsinanimalsexposedto20,000and50,000ppm.Themaineffectswereconcentration-relatedandoccurredintheheart(musclefibervacuolationandmononuclearcellinfiltrates)andliver(hepatocellularvacuolationandmononuclearcellinfiltrates)ofanimalsexposedto20,000and50,000ppmandinthenasalpassages(decreasedgobletcellexpression)ofanimalsexposedto50,000ppm.Thus5,000ppmwasconsideredtobetheNOELforHF0-1234ze(E)inthisstudyY1b)AGLP4-weekinhalationtoxicitystudywasconductedwithfivegroupsofmaleandfemaleSpragueDawleyratsexposednoseonlytovaporsofHF0-1234ze(E)atlevelsof0(control),1,000,5,000,10,000and15,000ppmfor6-hours/day,5days/weekduringa 4weekperiod,withatotalof20-21exposuredays.Asanadditionalcomponentofthisstudy,atnecropsy,livercellsfrommaleratsinthecontrol,5,000and15,000ppmgroupswereevaluatedinanUnscheduledDNASynthesisTestandbonemarrowfrommaleratsinthecontrol,5,000,10,000or15,000ppmgroupswasassessedinaMicronucleusTest.ResultsofthesetwogenotoxicityassaysarepresentedbelowundertheheadingGenotoxicity.Notreatment-relatedchangeswereobservedwhenclinicalobservations,bodyweightgain,foodconsumptionorfoodconversionefficiencywereevaluated.Althoughclinicalchemistryanalysisshowedsomevariations,theydidnotappearinaconcentrationrelatedpatternandthus,theywerenotconsideredtreatment-related.Atnecropsy,notreatment-relatedmacroscopicchangesorchanges inorganweightswere-20-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013found.Microscopicexaminationrevealedveryslighttomoderateinflammationoftheheartofmaleratsexposedto15,000ppm;twoofwhichalsoshowedmusclefibervacuolation.Basedontheseresults,10,000ppmwasconsideredtheNoObserved-Adverse-EffectLevel(NOAEL)fora4-weekexposuretoHF0-1234ze(E).1212)SubchronicToxicityAGLP13-weekinhalationtoxicitystudywasconductedwithfourgroupsof10maleand10femaleSpragueDawleyratsthatwereexposedtovaporsofHF0-1234ze(E)atlevelsof0(control),1,500,5,000,or15,000ppmfor6hours/day,5days/weekduringa13-weekperiod,withatotalnumberof63-64exposuredays.Notreatment-relatedchangeswereobservedwhenclinicalobservations,bodyweightgain,foodconsumptionorfoodconversionefficiencywereevaluated.Analysisofhematologyparametersandclinicalchemistryshowedsomeslightvariationsat15,000ppmwhichmaybetreatment-related.Atnecropsy,notreatment-relatedgrosschangeswereobservedduringmacroscopicexaminationandnotreatmentrelatedorganweightchangeswereobserved.However,microscopicexaminationrevealedmultifocalmononuclearcellinfiltratesintheheartofbothsexesat15,000ppm.Fibrosisofthecardiactissuewasnotobserved.Basedontheseresults,15,000ppmwasconsideredaLow-Observed-Adverse-EffectLevel(LOAEL)and5,000ppmwasconsideredtheNo-Observed-EffectLevel(NOEL)fora13-weekexposuretoHF0-1234ze(E).1313)ChronicToxicity/CarcinogenicityNochronicstudieswereidentified.4)Reproductive/DevelopmentalToxicitya)InaGLPdevelopmentaltoxicitystudy,groupsof24matedfemaleratswereexposednoseonly,tolevelsof0(control),1,500,5,000or15,000ppmofHF0-1234ze(E)for6hours/dayondays6-19ofgestation.Therewasnomortality.Noeffectwasseenonbodyweightorfoodconsumptionandclinicalobservationsforallgroupswereunremarkable.Therewerenosignificantdifferencesinfecundityindex,numberofcorporalutea,thenumberofimplantationsites,thenumberoflivefetuses,thepostimplantationloss,orsexratioofthepups.Inthepups,therewerenostatisticallysignificantdifferencesinvisceralorskeletalfindings.Itwas-21-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013concludedthattheno-observedeffectlevelwas15,000ppm,thehighestleveltested.(7)b)Aninhalationrange-findingprenataldevelopmentaltoxicitystudyofHF0-1234ze(E)inrabbitswasconductedforthepurposeofdeterminingappropriateexposureconcentrationsforadefinitiveprenataldevelopmentaltoxicitystudy.Fourgroupsoftime-matedfemaleNewZealandrabbits(6/group)wereexposedbywhole-bodyinhalationto0(control),1,500,5,000or15,000ppmHF0-1234ze(E)for6hours/dayduringgestationdays6to28.Allanimalssurvivedtotheschedulednecropsyongestationday29.Nosignsofmaternaltoxicitywereobserved.Intrauterinegrowthandsurvivalwereunaffectedbymaternalexposuretoallexposurelevels.Noexternalfetalmalformationsordevelopmentalvariationswerefound.Itwasconcludedthatinhalationexposuresupto15,000ppmHF0-1234ze(E)topregnantrabbitsdidnotcausematernalordevelopmentaltoxicityinthispilotstudy.181c)AGLPinhalationprenataldevelopmentaltoxicitystudyofHF0-1234ze(E)inrabbitswasconducted.Fourgroupsoftime-matedfemaleNewZealandrabbits(22/group)wereexposedbywhole-bodyinhalationto0(control),1,500,10,000or15,000ppmHF0-1234ze(E)for6hours/dayduringgestationdays6to28.Allanimalssurvivedtotheschedulednecropsyongestationday29.Nosignsofmaternaltoxicitywereobserved.Intrauterinegrowthandsurvivalwereunaffectedbymaternalexposuretoallexposurelevels.Itwasconcludedthatinhalationexposuresupto15,000ppmHF0-1234ze(E)topregnantrabbitsdidnotcausematernalordevelopmentaltoxicity.191d)AGLP2-generationreproductivestudywasconductedbyinhalationwithHF0-1234ze(E)inSprague-DawleyCri:CD(SD)rats.Groupsof24maleand24femaleratswereexposedtolevelsof0(control),2,000,5,000and20,000ppmofHF0-1234ze,7days/weekfor10weekspriortomating,duringmating,gestationuntilDay20(females)andfrom4dayspostpartumuntiltheendoflactation.Mortalityoccurredinfemalesinthehighexposurelevelgroup(20,000ppm)inboththeFoandF1generations.SevenfemalesintheFogenerationwereeitherfounddeadorsacrificedinextremuslateinthelactationphase.TwofemalesintheF1generationwerefounddeadonlactationdays14and15.OnefemaleintheFogenerationappearedtohaveabrainlesion,therewasnootherapparentcauseofdeathintheotherrats.TherewerenoothercompoundrelatedfindingsineithertheFoorF1generations,includingclinicalobservations,bodyweights,foodconsumption,matingorfertility,gestation,lactation,parturition,sexualmaturation,organweightsormicroscopic-22-HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013evaluations1101•TheNOELforsystemictoxicitywas5,000ppmandtheNOELforreproductiveeffectswas20,000ppm.5)Genotoxicity1.InVitroa.AnAmesassayscreenwasconductedwiththebacteriaSalmonellatyphimurium(strainsTA1535,TA1537,TA98,andTA100)andEscherichiacoli(strainWP2uvrA)bothwithandwithoutmetabolicactivationfromaratliverpreparation.Eachstrainwasexposedtoasingleexposurelevelof5%(50,000ppm)HFO1234ze(E).Undertheconditionsofthisassay,HF0-1234ze(E)didnotshowanyevidenceofmutagenicactivity.1111b.AGLPAmesassaywasconductedwithHF0-1234ze(E)whichinvolvedexposureofbacterialstrainsTA1535,TA1537,TA98,TA100andWP2uvrAbothwithandwithoutS-9metabolicactivation.Exposurelevelsofupto76%(plus19%02and5%C02)wereused.HF0-1234ze(E)didnotinducearesponseinanystraintestedeitherwithoutorinthepresenceofmetabolicactivation.1121c.AGLPChromosomeAberrationTestwasconductedwithculturedhumanlymphocytesthatwereexposedtovaporslevelsofHF0-1234ze(E)upto76%,bothwithandwithoutS-9metabolicactivation.Undertheconditionsofthistest,HF0-1234ze(E)wasnotclastogenic.11312.1nVivoa.AMouseMicronucleusAssaywasconductedfollowingasingle4-hourexposureto29,208ppmHF0-1234ze(E).At48and72hoursafterexposure,peripheralbloodsmearsampleswereobtainedfrom5maleand5femaleexposedmice,aswellasfromnegativeandpositivecontrolanimals.Itwasconcludedthata4-hourexposureto29,208ppmHF0-1234ze(E)didnotcausechromosomedamageintheperipheralbloodofexposedmice.1141b.AsecondMicronucleusAssaywasconductedwith10maleand10femaleCD-1micethatwereexposedbynose-onlyinhalationto103,300ppmHF0-1234ze(E)for4hours.At24-and48-hoursafterexposure,bonemarrowcells-23-HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013from5micepersexperintervalwerecollectedandanalyzedforthepresenceofmicronuclei.Nosignsoftoxicitywereobservedduringorafterexposure.ItwasconcludedthatHFO1234ze(E)wasnon-genotoxicatalevelof103,300ppm,asitdidnotcauseanincreaseinmicronucleiorevidenceofbonemarrowcelltoxicityinmice.15>c.AratMicronucleusTestwasincludedinthe4-weekinhalationtoxicitystudy(describedabove).InthatstudygroupsofmaleandfemaleSprague-DawleyratswereexposednoseonlytovaporsofHF0-1234ze(E)atlevelsof0(control),1,000,5,000,10,000or15,000ppm6-hours/day,5days/weekduringa4-weekperiod.Atnecropsy,bonemarrowfrommaleratsinthecontrol,5,000,10,000or15,000ppmgroupswasusedintheMicronucleusTest.Atthehighestconcentrationtested(15,000ppm),nodamagetochromosomesorincreasedincidenceofmicronucleiwasobservedinthebonemarrowcellsofmalerats.12>d.AnUnscheduledDNASynthesisTestwasalsoincludedinthe4-weekinhalationtoxicitystudy(describedabove).Atnecropsy,livercellsfrommaleratsinthecontrol,5,000and15,000ppmgroupswereusedintheUnscheduledDNASynthesisTest.Atthehighestconcentrationtested(15,000ppm),nounscheduledDNAsynthesiswasobservedinthelivercellsofmalerats.12>6)Metabolism/PharmacokineticsHF0-1234ze(E)didnotundergoanymeasurablemetabolismwhenratswereexposedtolevelsupto5%HF0-1234ze(E)forasingle4-hourperiod.115>7)GenomicsStatisticalanalysisofrodentgeneexpressionchangeswereconductedaspartofthecomprehensivetoxicologicalassessmentofHF0-1234ze(E).Thiswasnotaguidelinestudy,wasnotGLPcompliant,andisnotacceptedasavalidatedmethodbytheregulatoryauthorities;however,itwasconductedtoprovideadditionalinformation.Geneexpressionchangesinliver,kidneyandlungtissuewereassessedfollowingexposureoffemaleB6C3F1miceandmaleF344ratstolevelsof5,000and15,000ppmHF0-1234ze(E)6-hrs/day,5days/wkfor13weeks.Theassessmentwasbasedontheresultsfromacomparisonoftheresponsesseen-24-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013withHF0-1234ze(E)tobothpositive[tetrafluoroethylene,1-amino-2,4-dibromoanthraquinone,andTris(2,3-dibromopropyl)phosphate]andnegative[trichlorofluoromethane,iodoform,tetrafluoroethaneandN-(1-naphthyl)ethylenediaminedihydrochloride]controlsforrodenttumorigencity.Vehiclecontrolswerealsoincluded.Inadditionhistopathologicalexaminationofselectedtissueswasconducted.StatisticalclassificationanalysispredictedHF0-1234ze(E)tobenoncarcinogenicinbothfemalemouseliverandmaleratkidney.Apositiveresponsewasseenwiththefemalemouselung.Therewasnodoseresponserelationshipwithrespecttochanges ingeneexpression.Furthermore,asonlyalimitednumberofgeneswerealtered,itwasconcludedthatforthefemalemouselung,thenumberofgenesalteredwastoosmalltoperformameaningfulgeneontologyenrichmentanalysis.Notreatmentrelatedhistopathologicallesionswereobservedintheliverorkidneyandonly1/10miceinbothexposuregroupsshowedmildirritationinthelung.1161RECOMMENDEDWEELGUIDEAWEELOELhasbeenapprovedbytheWEELCommitteeforHF0-1234ze(E).Itrecommends800ppmasan8-hourTWA.1171MATERIALSAFETYDATASHEETAHoneywellMaterialSafetyDataSheetforSolsticeTMZERefrigerantisattached.1181RECOMMENDEDATELforHFC-1234ze(E)4-hrLC50>207,000ppm(v/v)CardiacsensitizationNOEL:>120,000ppm(v/v)Anesthetic/CNSeffectNOEL:>207,000ppm(v/v)80%>166,000ppm(v/v)Otherescapeimpairingeffects:Nonehavebeenidentified.OxygenDeprivationLimit:140,000ppm,perSection7.1.2ofStandard34-2010.ATEL:59,000ppm(v/v)REFERENCES(1)Subacute(2-week)InhalationToxicityStudywithHF0-1234zeinrats.TNOStudy6456,25August2006,TNOQualityofLife,Zeist,TheNetherlands-conductedforHoneywellInternational,ref.MA-RR-06-2838.(2)Sub-acute(4-week)InhalationToxicityStudy,includingUnscheduledDNA-25-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013SynthesisandMicronucleustest),withHF0-1234zeinrats.TNOStudy6747,11December2006,TNOQualityofLife,Zeist,TheNetherlands-conductedforHoneywellInternational,ref.MA-RR-06-2840.(3)Sub-chronic(13-week)InhalationToxicityStudywithHF0-1234zeinrats.TNOStudy7383,28March2008,TNOQualityofLife,Zeist,TheNetherlands-conductedforHoneywellInternational,ref.MA-RR-08-4095.(4)PrimaryDermalIrritation/CorrosioninRabbits:HF0-1234ze.MBResearchLaboratoriesStudyMB-09-18677.03,Spinnertown,PAconductedforHoneywellInternationalref.MA-RR-10-4295.(5)AnAcute(4-hour)InhalationToxicityScreeningStudywithaMicronucleusAssayintheMouseandRatviaNose-onlyExposure.StudyNo.03-5472,30March2004,HuntingdonLifeScience,EastMillstone,NJconductedforHoneywellInternational,ref.MA-RR-04-2768.(6)AcuteCardiacSensitizationStudyofHF0-1234zeandHF0-1234yfinDogs.WILStudyNo.WIL-447008,23August,2006WILResearchLaboratory,Ashland,OhioconductedforHoneywellInternational,ref.MA-RR-06-2806.(7)PrenatalDevelopmentalInhalationToxicityStudywithHF0-1234zeinRats.TNOStudyV7384.TNOQualityofLife,Zeist,TheNetherlandsconductedforHoneywellInternational,ref.MA-RR-08-4004.(8)AnInhalationRange-FindingPrenatalDevelopmentalToxicityStudyofHF0-1234ze(1,1,1,3-Tetrafluoropropene)inRabbits.WILStudyNo.WIL-447020,WILResearchLaboratories,LLC,Ashland,Ohio44805conductedforHoneywellInternational,ref.MA-RR-08-4092.(9)HF0-1234ze:Embryo-fetalToxicityStudyinRabbitsviaWhole-bodyInhalationExposure.HLSStudyNo.09-4350,HuntingdonLifeScience,EastMillstone,NJ,May2010,conductedforHoneywellInternationalref.MA-RR-10-4302.(10)HF0-1234ze:Two-generationreproductiveperformancestudybyinhalationadministrationtoCDrats.HLSStudyNo.NBJ0085,HuntingdonLifeSciences,Ltd.,Huntingdon,Cambridgeshire,PE186ES,EnglandconductedforHoneywellInternational,ref.MA-RR-13-4361(19March2013).-26-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)(11)HF0-1234zeTrans(508-96A):BacterialMutationAssay,StudyNo.ALS129/961626,18August1997,HuntingdonLifeSciences,Ltd.,Huntingdon,Cambridgeshire,PE186ES,EnglandconductedforHoneywellInternational,ref.MA-RR-97-2392.December2013(12)MutagenicityTestof(E)-1,3,3,3-Tetrafluoroprop-1-eneUsingMicroorganisms.JapanBioassayResearchCenterStudy6301,conductedforHoneywellInternational,Jan.26,2009.ref.MA-RR-09-4266(13)ChromosomeAberrationTestwithHF0-1234zeinCulturedHumanLymphocytes.TNOStudy6202/08,8November2005,TNOQualityofLife,Zeist,TheNetherlands-conductedforHoneywellInternational,ref.MA-RR-05-2810.(14)HF0-1234ze,cis(512-96A):MousePeripheralBloodMicronucleusTest,Huntingdon,reportdateJuly3,1997,conductedforHoneywellInternational,ref.MA-RR-97-2380.(15)P.Schuster,R.Betermann,G.M.RuschandW.Dekant.Biotransformationoftrans-1,3,3,3-tetrafluyoropropene(HF0-1234ze).ToxicologyandAppliedPharmacology,inpress(2009).(16)ToxicologicalAssessmentoftheCarcinogenicPotentialofHF0-1234ze.CIITReport06014,HamnerInstitutesforHealthSciences,ResearchTrianglePark,NC,conductedforHoneywellInternational,ref.MA-RR-07-3453(January5,2007).(17)1,3,3,3-tetrafluoropropene.WorkplaceEnvironmentalExposureLevel.OccupationalAllianceforRiskScience(OARS),WorkplaceEnvironmentalExposureLevels(WEEL),(2013).(18)Honeywell(August10,2012).MaterialSafetyDataSheetSolsticeTMZERefrigerant,HoneywellInternational,Inc.,101ColumbiaRoad,Morristown,NJ07960.-27-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013ToxicityInformationBlendR-134af/R1234ze(E)(41.0/59.0%bymass)AcuteToxicitya.ACGIHTLV-c:Notestablishedb.ACGIHTLV-STEL:Notestablishedc.NIOSHIDLH:Notestablishedd.4-hrLC50rat=Nodataavailablee.LDso:Notapplicabletogasf.Cardiacsensitization:NodataavailableChronicToxicitya.ACGIHTLV-TWAorTVL-C:Noneb.AIHAWEEL:Nonec.OSHAPELorRecommendedExposureLevel880ppmv/v(BlendTLV-TWAisbasedontheAIHAWEELvaluesforHFC-134aandHF0-1234ze(E).ToxicitywasconsideredadditiveandthecalculationmethodisthatdescribedforThresholdLimitValuesforMixtures(ACGIH,2008).RecommendedBlendATELforASHRAEStandard34:72,000ppmv/v(Seetablebelowforinputvalues.)CardiacLethalitySensitizationAnestheticEffectsComponent4-hrLCsoNOELLOELECsoNOELLOELppmv/vppmv/vppmv/vppmv/vppmv/vppmv/vHFC-134a>359,00050,000----------270,000---------------------HF0-1234ze(E)>207,000>120,000------------------------------207,000-28-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Section4:FlammabilityInformation-29-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)FlammabilityInformation(ASHRAEStd34-2010Section9.7)FlamePropagationFlammabilityTesting-Appendix81ConclusionDecember2013TheWCFandWCFFweretestedforflammabilityaccordingtoASTME681-2009testprocedureasrequiredinASHRAEStandard34-2010anddescribedinAppendix81toASHRAEStandard34-2010.TestconditionswereRTand60°CfortheWCFandWCFF.Airusedhadahumidityof0.0088gmwatervapor/gmdryairwhichequatesto50%relativehumidityat23.0°Cand1atm.R-134a/1234ze(E)wasfoundtobenon-flammableattheWCFandWCFFwhentestedatambientand60°Cinair,1atmpressure,50%RH(at23°C).Aheatofcombustionof8.69MJ/kgwascalculatedusingthecalculationmethodofASHRAE34-2010.Thisdataanticipatesaflammabilityclassificationof1.MethodThetestingwasperformedperASHRAE'scurrentStandard34-2010testprotocol(conditionandapparatus)usingthecurrentmethodASTME681-04annexA1.Thetestingwasperformedwiththe0.4seconddurationsparkfromthesecondaryofa15kV30rnAilluminationtransformer.TheelectrodeswereL-shaped1mmtungstenwirewithagapof%inch.Theigniterwasplacedabovethebottomoftheflaskataheightequivalentto1/3diameteroftheflask.Thevesselwasa12-literflask.Thetestconditionswereambientandaooctemperaturesusing0.0088gwater/gdryair(50%RHairat23°C,dewpointof10.9-12.6°C}.Flamepropagationisdefinedasflamesthathavingspreadupwardandoutwardtothewallsoftheflask,arecontinuousalonganarcthatisgreaterthanthatsubtendedbyanangleequalto90°,asmeasuredfromthepointofignitiontothewallsoftheflask.Theflameshallbecontinuousalongtheflaskwall.SeeFigure4.1.Mixturesweremadebyevacuatingtheflaskandusingpartialpressuresinfillingtothedesiredconcentration.Theairwasintroducedrapidlytoassistinmixingandallowedtocometotemperatureequilibrium.Pressurewasmeasuredusingapressuretransducerreadingtoanaccurracyof+/-0.3mmHg.Atwominutepausewasallowedaftermixingtoallowthemixturetostagnatebeforeignitionwasattempted.Figure4.1E681flammabilityflaskandburn/noburncriteria-30-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013Theexpectedmostlikelytobeflammablecompositionisaround9.3vol%inair.Thisisnearthestoichiometricconcentrationin50%RHair.MaterialThematerialusedwas99.8%orbetterpurityandwasdegassedbyrepeatedfreeze-pumpedthawcyclesuntilnoevidenceofairwasseenonavacuumgage.Therangeofcompositionsinairtestedwassuchthatifitwerepossible,flamepropagationwouldhaveoccurred.Table4.1CompositionofWCFandWCFFtestedforflammabilityGoal,weight%Asdeterminedbymass,weight%NorminalR-134a42R-1234ze(E)59WCFR-134a4039.9R-1234ze(E)6060.1WCFFR-134a17.316.6R-1234ze(E)82.783.4TheWCFF,bythelargestamountofflammable,R-1234ze(E),isthefinalliquidatboilingpoint+10ocwhichis-13.2°C.ExperimentalResultsOurinternaltestingonR-134a/1234ze(E)flammabilityhasshown,bystrictadherencetoASHRAE34,tobeflammabilityclass1.R-134a/1234ze(E)Flammabilitytestingat(50%RH),12literflask,sparkignition,ambientpressureof740-750mmHgWCFFatsoocusing50%RHat23°CairR-134a/1234ze(E)(17.3/82.7mass%)Table4.2WCFFflammabilitytesting-31-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013Table4-2R134a/1234ze(E)WCFF60°CflammabilitytestingRefrigerantAirWCFFBarometerResultMol%MOLE%T"CAngleommHg964600748Nonflammable955600748Nonflammable946600746Nonflammable937600746Nonflammable928600746Nonflammable919600746Nonflammable9010600746Nonflammable8911600746Nonflammable8812600746Nonflammable8713600746Nonflammable8614600746Nonflammable8515600743NonflammableR-134a/1234ze(E)Flammabilitytestingat(50%RH)12literflask,sparkignition,ambientpressureof756mmHgWCFat60°Cusing50%RHat23°CairR-134a/1234ze(E)(39.9/60.1mass%)Table4.3WCFflammabilitytestingTable4-3R134a/1234ze(E)WCF60°CflammabilitytestingRefrigerantAirWCFFBarometerResultMol%MOLE%T"CAngleommHg964600756Nonflammable955600756Nonflammable946600756Nonflammable937600756Nonflammable928600756Nonflammable919600756Nonflammable9010600756Nonflammable8911600756Nonflammable8812600746Nonflammable8713600746Nonflammable8614600756Nonflammable8515600756NonflammableValidationofTestAccuracyConfirmationofmethodandtestresultsemployedinthisApplication:In1996-1997,anindustryroundrobintestingprogramwasinitiatedtoassesstheinter-labvariabilityofflammabilitymeasurementsusingtheE681methodreferencedinthisStandard.ThisE681methodandapparatusweretestedusinga40%/60%R32/R134ablendflammabilitycharacterizationto-32-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013compareresultswithotherASHRAEaffiliatedlaboratories.AlliedSignal(Honeywell)foundaCFRof32.5wt%R-32.Theroundrobinresultwas33.4±1.2wt%.TheflamelimitsofR-32/R-134a40/60wt%werere-measuredbyHoneywellinNovember,2007andfoundtobe13.0-18.0val%in50%RHair,whilethepreviousroundrobinfound13.9±0.9%-17.9±0.5%in50%RHair.UnderwritersLaboratoriesandDuPontFluoroproductsalsoparticipatedintheroundrobinstudyandtheirE681resultsfortheCFRandflamelimitsoftheblendwerealsowithintheinter-labstandarddeviations.Therefore,thereissupportingevidencethattheresultsandmethodusedinthisApplicationwillmeetthequalityrequirementsofStandard34.FractionationFlammabilityInformation(ASHRAEStd34-2010Section9.7)FractionationAnalysis(ASHRAEStandard34-2010Appendix82)FractionationanalysiswascarriedoutaccordingtoinstructionsinAppendix82toASHRAEStandard34-2010.Theinitialanalysisincludedstorageleaksat90%fill(ofmaximumDOTfillvolume)at-40°Corthenormalboilingpointplus10°C,23°Cand54.4°Ctemperatures,equipmentleaksat15%fill(ofmaximumDOTfillvolume)at-40°Corthenormalboilingpointplus10°C,23°Candsooctemperatures,andcharge/rechargethrough5leakageeventsof20%ofeachrefrigerantcharge.Themodelcalculateddataandexperimentalverificationdataarepresentedinthefollowingtablesandgraphs.FractionationoftheR-134a/1234ze(E)(40/60wt.%)WCFtogivetheWCFFR-134a/1234ze(E)(82.7/17.3wt.%),finalliquidatthelowesttemperature:boilingpointplus10°C(-13.2°C)seeFigure4.2.Figure4.2TemperatureforWCFFbymaximumflammable.84.0%83.0%82.0%81.0%80.0%79.0%78.0%77.0%76.0%75.0%74.0%73.0%-20............-10WCFFR1234ze(E)liq..............................010...............""20t("C)""30"".405060RefProp9.1InteractionparameterforR-134a/1234ze(E)VaporLiquidData(VLE)wasobtainedfrompressuretemperaturebulkcomposition(PTz)measurementsfrom0to50"Cand50to100%bywtR-1234ze(E)withR-134a.Fromthisdatatheinteractionparametersweredetermined.Azetaof-6.2782wasdeterminedandusedforthesimulations.-33-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013Table4-4R-134a/1234ze(E)PTzdatafordetermininRefProp9.1interactionparameterBulkBulkMeasured MeasuredBulkBulkMeasured MeasuredR·R·134aTempPressureR·R·134aTempPressure1234ze(E)1234ze(E)(g)(g)•cpsi(g)(g)·cpsi39.40-0.24579731.23252639.49.20-0.22158434.54423539.409.596944.32706839.49.209.58908448.67341839.4019.6316161.47191839.49.2019.62688467.26183239.4029.78356583.68434139.49.2029.79632691.08951539.4039.779942110.5362339.49.2039.912822120.4138739.40.3-0.18337131.30417939.412.00-0.05800635.26753339.40.39.67326744.50926939.412.009.68117749.60224739.40.319.66657961.8451639.412.0019.72049168.44184839.40.329.85589183.97702139.412.0029.88403592.62652239.40.339.900707110.9541839.412.0039.953619122.2424139.40.8-0.05522831.5779839.413.00-0.00059435.33787739.40.89.80720444.82660539.413.009.78167749.8975739.40.819.81356662.11196839.413.0019.79361769.05077739.40.829.95358184.33138239.413.0029.93108993.43688139.40.839.975435111.0449439.413.0040.020288123.3240639.41.50-0.14708631.85633639.413.50-0.13713235.46953839.41.509.71553445.17782439.413.509.74452150.21168239.41.5019.72675462.54999139.413.5019.69194269.135m39.41.5029.80407685.01462339.413.5029.88600693.48485539.41.5039.873999112.2103839.413.5039.902178123.3889839.42.90-0.13952732.610339.418.60-0.18610136.30554939.42.909.m64246.14658139.418.609.62284651.21688739.42.9019.717563.73106839.418.6019.67898870.69250239.42.9029.86729486.40246439.418.6029.82301495.71599939.42.9039.899089113.4891539.418.6039.941814126.4546639.43.50-0.16897432.78240939.424.00-0.14292637.16973339.43.509.59809346.32046839.424.009.59992752.169439.43.5019.63797264.12574539.424.0019.66153872.06708239.43.5029.78673487.01856739.424.0029.84010897.50243439.43.5039.89027115.0221639.424.0039.871917128.7656639.44.70-0.23192333.13656539.429.00-0.30423537.26510139.44.709.71637546.95898139.429.009.77006652.99732939.44.7019.72581664.90754339.429.0019.81362473.1983639.44.7029.89502387.83103639.429.0029.88906298.74734239.44.7039.885432115.694539.429.0039.902654130.2032939.45.60-0.11074433.35709639.432.100.07828537.87158239.45.609.67634347.16859439.432.109.72632953.16544339.45.6019.70256665.27368539.432.1019.64159673.55310939.45.6029.93294488.41579339.432.1029.82921999.47041639.45.6039.96353116.7479939.432.1039.91474131.2665239.45.90-0.1328233.52177439.435.60-0.27837837.90619239.45.909.71980847.31193139.435.609.64478553.53702239.45.9019.73940565.40899639.435.6019.69128674.10369939.45.9029.90573888.69937139.435.6029.856418100.1835439.45.9039.9917117.2333839.435.6039.850385131.9677439.46.40-0.1757533.64366239.440.50-0.2372538.22512639.46.409.67489547.57536439.440.509.73233454.28606539.46.4019.69225365.77969339.440.5019.68922874.86183539.46.4029.83167889.08943339.440.5029.858518101.1657939.46.4039.836632117.6051539.440.5039.861647133.5981639.47.40-0.10601933.93489139.47.409.68054747.82270539.47.4019.71159966.15647739.47.4029.79906189.66189839.47.4039.7876117.99261-34-HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Table4.5Simulatedcylindervaporleak@--13.2°C(Bpt+10°C)(initial90%fill)-13.21oCLiquidphaseVaporphaseLeak%PressureMass%Mass%MPaR-1234ze(E)R-134aR-1234ze(E)R134a00.15375760.02%39.98%52.35%47.65%20.1536860.17%39.83%52.50%47.50%100.15335260.84%39.16%53.14%46.86%200.15289361.75%38.25%54.04%45.96%300.15236462.80%37.20%55.07%44.93%400.15174364.01%35.99%56.26%43.74%500.15099565.44%34.56%57.70%42.30%600.15006167.20%32.80%59.48%40.52%700.14883169.46%30.54%61.81%38.19%800.14705772.60%27.40%65.13%34.87%900.14396177.81%22.19%70.87%29.13%950.14087382.69%17.31%76.54%23.46%-35-December2013HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Figures4.3and4.4Simulatedcylindervaporleak@-13.2"C(Bpt+10"C)(initial90%fill)90.0%-----------------------------------------------------------------70.0%----------------60.0%:;::8500%E.,,g0.v20.0%10.0%0.0%'0.0%10.0%20.0%30.0%40.0%50.0%60.0%70.0"/030.0%90.0%100.:J%Leak'1Vt0o90.0%80.0%70.0%60.0%.o::50.0%8-E,,40.0%.._,_____0e.§u30.0%20.0%10.0%U.U%.J----,-----,---------,-----,.--0.0')(10.0%20.0%30.0%40.0%50.0%60.0'!f70.0%80.0%90.0%100.0%Leakwt"'o-36-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Table4.6Simulatedcylindervaporleak@23°C(initial90%fill)23.00°CLiquidphaseVaporphaseLeak%PressureMass%Mass%MPaR-1234ze(E)R-134aR-1234ze(E)00.55071261.00%39.00%54.63%20.55048961.13%38.87%54.76%100.54954761.69%38.31%55.32%200.54823562.46%37.54%56.08%300.54673363.34%36.66%56.96%400.54498664.35%35.65%57.97%500.54290265.54%34.46%59.18%600.54033266.98%33.02%60.65%700.53700868.82%31.18%62.55%800.53235271.34%28.66%65.19%-37-December2013R-134a45.37%45.24%44.68%43.92%43.04%42.03%40.82%39.35%37.45%34.81%HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Figures4.5and4.6Simulatedcylindervaporleak@23°C(initial90%fill)90.0%--LiquidphaseMassJ=racR1234ze80.0%·------------------------70.0%060.0%.-c50.0%-s'"0&40.0%.l0u30.0%I20.0%10.0%0.0%0.0%20.0%40.0%60.0%80.0%100.0%Leakwt%80.0%70.0%+-60.0%0-vaj)OirphaseMassFtac.R134.a50.0%;;......-0'_z:j40.0%-'"0::::..>::030.0%u20.0%10.0%0.0%0.0%20.0%40.0%60.0%80.0%100.0%Leakwt%-38-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Table4.7Simulatedcylindervaporleak@54.4°C(initial90%fill)Vapor54.40°CLiquidphasephaseLeak%PressureMass%Mass%MPaR-1234ze(E)R-134aR-1234ze(E)R-134a01.29625760.04%39.96%55.00%45.00%21.29584560.15%39.85%55.11%44.89%101.29410360.61%39.39%55.56%44.44%201.29167861.25%38.75%56.20%43.80%301.28890961.97%38.03%56.92%43.08%401.28568862.81%37.19%57.76%42.24%501.28184663.80%36.20%58.76%41.24%601.27710265.02%34.98%59.99%40.01%701.27092966.57%33.43%61.57%38.43%801.26215368.75%31.25%63.81%36.19%901.24712972.37%27.63%67.60%32.40%-39-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Figures4.7and4.8Simulatedcylindervaporleak@54.4°C(initial90%fill)80.0%70.0%-Licpi:lplwe60.0%Li.qui:!Fmc:i:3-111,c50.•}%2::."'.§40.0%:;a.§u30.0%"""lO.O%-------lO.O%I0.0%-,--------,--,-'---,-O.J%l:J.O'K20.0%30.0%40.0%50.0%60.0':1,70.0%80.0".490.0%Leak\\1%8[).0Jb70.0li.----60.0%*-Ic::I-----0e.40.0Yo5u30.0%-Il:l.U%I'O.O'Xr----.--lv1G'e_0,16O.O'l'<10.0%20.0%3D.O'Yt40.0%70.C%80.0%90.0'!>:oo.o?>-40-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)ITable4.8Simulatedequipmentvaporleak@-13.2°C(Bpt+10°C)(initial15%fill)Vapor-13.21oCLiquidphasephaseLeak%PressureMass%Mass%MPaR-1234ze(E)R-134aR-1234ze(E)R-134a00.15359460.35%39.65%52.67%47.33%20.15351660.51%39.49%52.82%47.18%100.15318761.17%38.83%53.47%46.53%200.15272562.09%37.91%54.37%45.63%300.15219363.13%36.87%55.40%44.60%400.1515764.34%35.66%56.60%43.40%500.15082165.77%34.23%58.03%41.97%600.14988767.52%32.48%59.81%40.19%700.14866269.76%30.24%62.12%37.88%800.14691172.86%27.14%65.41%34.59%900.14393377.86%22.14%70.92%29.08%950.14115982.25%17.75%76.02%23.98%-41-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013Figures4.9and4.10Simulatedequipmentvaporleak@-13.2°C(Bpt+10°C)(initial15%fill)....§·-=•r.0=-:::;8.o0"§'"0§<0u90.0%80.0%70.0%Ir60.0%SO.O'!o40.0%30.0%20.0%10.0%0.0%·1-----r---..----r------,.-----G.Oo/..10.0",{20.0'¥.30.0\i4J.Ol050.0%60.0%70.J%80.0"/o90.0%103.0%Leak80.0%70.0%60.0%50.0%40.0%1--------30.0%20.0%10.0%·1---------------------------0.0%0.0%10.0%20.0%30.0%40.0%50.0%60.0%70.0%80.0%90.0%100.0%Leakwt%-42-HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Table4.9Simulatedequipmentvaporleak@23°C(initial15%fill)23.ooocLiquidphaseVaporphaseLeak%PressureMass%Mass%MPaR-1234ze(E)R-134aR-1234ze(E)00.55071261.00%39.00%54.63%20.55048961.13%38.87%54.76%100.54954761.69%38.31%55.32%200.54823562.46%37.54%56.08%300.54673363.34%36.66%56.96%400.54498664.35%35.65%57.97%500.54290265.54%34.46%59.18%600.54033266.98%33.02%60.65%700.53700868.82%31.18%62.55%800.53235271.34%28.66%65.19%-43-December2013R-134a45.37%45.24%44.68%43.92%43.04%42.03%40.82%39.35%37.45%34.81%HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Figures4.11and4.12Simulatedequipmentvaporleak@23°C(initial15%fill)R!diYn-------------------------·------L.q.ai:lpbsue).{au:Frac.R1234::e70.0\<.-----L:qJi:iphaseMauFratR13La""[j:::+1----------·----------m0§30.0<;(;ulJQ.(}%--(•.0%L---,0.:>%10.0%20.0%30.0%40.0%50.0%60.0%80.0YoLeakwt•.•,...[60.0%-50.0%--VaporphaseMassFrac.R134a040.0%....0a.::::30.0%-----0u20.0%10.0%0.0%IT0.0%10.0%20.0%30.0%40.0%50.0%60.0%70.0%80.0%Leakwt'lo-44-90.0%90.0%December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0o/obymass)Table4.10Simulatedequipmentvaporleak@60°C(initial15%fill)Vapor60.0°CLiquidphasephaseLeak%pMass%Mass%MPaR-1234ze(E)R-134aR-1234ze(E)R-134a01.47378862.19%37.81%57.41%42.59%21.47333162.30%37.70%57.51%42.49%101.47140562.74%37.26%57.96%42.04%201.46873463.34%36.66%58.57%41.43%301.465764.03%35.97%59.26%40.74%401.46219464.82%35.18%60.05%39.95%501.45805265.74%34.26%60.99%39.01%-45-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Figures4.13and4.14Simulatedequipmentvaporleak@60°C(initial15%fill)1.;SCIliSl!lfmcR1234:e60.1)%'SO.O%1.40.0%f0.0:§'30.0'JGsu20.()1010.0%IO.()l(,0.0%IOOl>10.0'1440.0%SO.O"-'GO.Ole,70.00.660.0%t50.0%I,oO'§40.0%I.::llil030.0%+--u20.0%I10.0%l0.0%0.0%10.0%20.0%30.0%40.0%50.0%60.0%-46-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0o/obymass)December2013Table4.11SimulatedequipmentCharge/Rechargevaporleak@23°C(initial15%fill)Vapor23°CLiquidPhasePhaseLeakpMassFrac.MassFrac.MPaR-1234ze(E)R-134aR-1234ze(E)R-134a0%0.55071261.00%39.00%54.63%45.37%10%0.54954761.69%38.31%55.32%44.68%20%0.54823562.46%37.54%56.08%43.92%20%0.54912361.94%38.06%55.56%44.44%30%0.54794762.63%37.37%56.25%43.75%40%0.54662263.40%36.60%57.02%42.98%40%0.5478462.69%37.31%56.31%43.69%50%0.54665563.38%36.62%57.00%43.00%60%0.54532164.15%35.85%57.78%42.22%60%0.54680663.29%36.71%56.91%43.09%70%0.54561463.98%36.02%57.61%42.39%80%0.54427464.75%35.25%58.38%41.62%80%0.54597563.78%36.22%57.40%42.60%90%0.54477864.46%35.54%58.09%41.91%100%0.54343265.23%34.77%58.87%41.13%-47-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013Figures4.15and4.16Simulatedequipmentcharge/rechargevaporleak@23°C(initial15%fill)70.0%60.0%-LiquidPhaseMassFrac.R1234:ze......50.0%::::-LiquidPhaseMassFrac.R134a.....040.0%.....--0.-4rn0-30.0%...........0C)20.0%10.0%0.0%0.00.20.40.60.81.0Leakwt%70.0%60.0%-1-VaporPhaseMassFrac.R1234ze--50.0%-1'.....I.gW.O%-!•vaDorPbaseMassFrac.R134a....rn0§30.0%1....0C)20.0%10.0%0.0%0.00.20.40.60.81.0Leakwt%-48-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)FractionationAnalysis-LabTestProcedureTestmixtureTherefrigerantmixturetobeevaluatedwastheWCFofR-134a/1234ze(E).December2013Testapparatusforworstcasefractionatedformulation(WCFFmodelvalidation)ThevesseltoholdtherefrigerantmixturewasaStainlessSteelcellof836cubiccentimeterinternalvolume,fittedwithliquidandvaporsamplingportandvalve.Thecellwasmaintainedatthedesiredtemperaturebyhousinginanenvironmentalchamber.Oncethetestvesselhadbeenchargedwithrefrigerant,itwasbroughttotheappropriatetemperature.Initialliquidandvaporsamplesweretakenforgaschromatographic(GC)analysis.Therefrigerantvesselinsidetheconstanttemperaturebathwasconnectedtoamassflowmeter.Therefrigerantwasleakedatanominalrateof2%ofthetotalmasschargedperhour.Thiscontinueduntiltheinternalpressureofthevesselwasatambientpressureornoliquidphaseremained,or95%ofthemasshadleaked.Liquidandvaporsampleswereanalyzedforcompositionfollowingtheprocedurebelow.ProcedureforFractionationCompositionAnalysisofR-134a/1234ze(E):•Preparetherefrigerantblendsampletotheexactspecificationinastainlesssteelcylindersuchthatthetestvesselwillbeat90%ofDOTfill.•TheleakingvaporwassampledandaGCwasusedtodeterminecomposition..•Theleakwasstoppedandliquidsampledatabout10%weightlossintervals.Analysis:Thesamplewasanalyzedinthevaporphasetodeterminetcompositionmoreaccurately,avoidinganyVLEfromcondensateordew.DeterminationofcompositionbyGasChromatography:GCequippedwithathermalconductivitydetector(TCD)wasused.TheGCcolumnwasapackedcolumn,ChromosorbP80/1001/8"10m,heliumcarriergas.GCMethod:Initialtemp.50.CInitialtime10minRate1 10deg/minFinalTemp.150degHoldtime0minInjectortemp200degDetector300degDetectortypeTCDPressure51.3psiDataAcquisition:PerkinElmerTotalchromCompositionCalculation:Thenormalizedweight%ofeachcomponentintheblendat-13.5°Cwascomputedfromacomparisonoftheirindividualpeakareaswiththoseoftherespectivecalibrationstandards.GCstandardsweremadeforthe-49-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013R134a/1234ze(E)mixture,withaccuraciesofbetterthan0.3%determinedfromthemassesofeachused.CalibrationoftheGCwasaccomplishedusingthreecalibrationstandardspreparedatthreedifferentcompositionsofthemixture.TheexperimentalverificationoftheRefPropmodelstartedwithaninitialR-134a/1234ze(E)compositionof40.5/59.5weight%.ThiscompositionisclosetotheWCFR-134a/1234ze(E)compositionof40/60weight%atatemperatureof-13.5°C,whichisclosetothe-13.2°CtemperatureoftheWCFF.PlottedbelowistheR-134a/1234ze(E)experimentaldataandREFPROP9.1output.ThisdataagreeswellwithREFPROP9.1.Experimentswerecarriedoutat-13.5°CandtheresultsverifyREFPROP9.1withtheexperimentallydeterminedinteractionparametersasasuitablemodelforthissystem.Table4.12ExperimentalfractionationdataexperimentaldataR-134aR-1234ze(E)R-134aR-1234ze(E)wt%lossliqliqvapvap0.00%40.44%59.56%47.33%52.67%1.95%40.43%59.57%47.24%52.76%8.95%39.83%60.17%46.69%53.31%17.43%39.14%60.86%46.14%53.86%26.41%38.32%61.68%45.27%54.73%35.64%37.31%62.69%44.28%55.72%44.94%36.24%63.76%43.14%56.86%54.24%34.90%65.10%41.86%58.14%64.14%32.97%67.03%40.10%59.90%73.32%30.89%69.11%37.77%62.23%Table4.13SimulatedRefProp9.1dataatthesametemperatureastheExperimentalfractionationdata-13.50oCLiquidphaseVaporphaseLeak%PressureMass%Mass%MPaR-1234ze(E)R-134aR-1234ze(E)R-134a00.14144759.52%40.48%51.80%48.20%20.14137659.67%40.33%51.95%48.05%100.14107360.34%39.66%52.59%47.41%200.14064961.27%38.73%53.49%46.51%300.1401662.32%37.68%54.52%45.48%400.13958663.54%36.46%55.72%44.28%500.13889364.99%35.01%57.16%42.84%600.13802866.76%33.24%58.95%41.05%700.13688569.04%30.96%61.30%38.70%800.13523472.23%27.77%64.66%35.34%900.13234277.52%22.48%70.47%29.53%950.12944482.49%17.51%76.23%23.77%-50-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Figures4.17and4.18Comparisonofexperimentalandsimulationmodeldata90.0%-LttdMassFrac.R1234z:e--LiqutdpbaseMassFracR134a80.0%70.0%i60.0%?:....50.0%I...0.•..•v0C'....._g0;a.§C)--......___.30.0%.,--..----------------------=,......=--------20.0%10.0%0.0%0.0%10.0%20()",(,30.0%40.0%50.0%60.0%700%80.0%90.0%1000%80.0%70.0%60.0%50.0%40.0%30.0%20.0%-!---Leakwt%MassFrac-R1234zeVaporphaseMassFracR134a---------------------------------------------10.0%+----------------------------------------------------0.0%0.0%10.0%20.0%30.0%40.0%50.0%60.0%70.0%80.0%90.0%100.0%Leak'"i'!·o-51-December2013HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)HeatofCombustionDecember2013PerASHRAEStandard34-2010section6.1.3.5,theheatofcombustionshallbecalculatedforconditionsof25°C(7rF)and101.3kPa(14.7psia).InaccordancewiththeaforementionedandASHRAEStandard34-201addemdumad,theheatofcombustioncalculationgivesavalueof8.69MJ/kgforR-134a/1234ze(E).Ifthereisinsufficienthydrogen(H)availablefortheformationofHF(g),HCI(g),andH20(g),thentheformationofHF(g)takespreferenceovertheformationofHCI(g),whichtakespreferenceovertheformationofH20.Ifthereisinsufficienthydrogenavailableforallofthefluorine(F)toformHF(g),thentheremainingfluorineproducesCOF2(g)inpreferenceofcarbon(C)formingC02.Anyremainingchloride(CI)producesCl2(g)(chlorine).0.447325C2H2F4+0.5526745317C3H2F4+2.05267202=>1.552672C02+2HF+1COF2TheheatofreactionforthecombustionofR-134a/1234ze(E)is0.447325*(-895.376)+0.552674*(-816.05)-1.552672*(-393.51)-2*(-273.3)-1*(-638.9)=944.96kJ/molR134a/R1234ze(E)nominalhasamolecularweightof108.67g/molThisgivesaheatofcombustionofkcal/g=8.69MJ/kg.-52-HoneywellApplicationtoANSIIASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)December2013Section5:RCLValueIContaminantsandImpurities-53-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)IRefrigerantData(ASNI/ASHRAEStandard34-2010Sections7.5/9.8December2013RefrigerantConcentrationLimit(ANSI/ASHRAEStandard34-2010Section7.5)ATEL:72,000ppm(v/v)ODL(OxygenDeprivationLimit):140,000ppm,perSection7.1.2ofStandard34-2010.LFL:noneBurningVelocity:notapplicableRCL:72,000ppm(v/v)ContaminantsandImpurities(ANSI/ASHRAEStandard34-2010Section9.8)ToxicityandFlammability:Therearenoknowncontaminantsorimpurities,includingisomericanddecompositionimpuritiesfrommanufacturing,transport,andstorageknowntoincreasetheflammabilityortoxicitywithintheprecisionoftheRCL.-54-HoneywellApplicationtoANSI/ASHRAEStandard34R-134a/R-1234ze(E)(42.0/58.0%bymass)Section6 -AppendicesAppendix1:MSDS,SubjectBlendHoneywellSolsticerMN13(HDR-15)Appendix2:MSDS,R-134aAppendix3:MSDS,R-1234ze(E)•55.December2013