Acequinocyl Summary of Analytical Chemistry and Residue Data **UNITED STA**

**TES ENVIRONMENTA L PROTECTION A** **GENCY**

**MEMORANDUM Date:**  30 - NOV - 201 1 **SUBJECT: Acequinocyl**  **.** P etition for

Shelled Beans; Cowpea Forage; Caneberry Subgroup 13 9A; Cucumber, Cherry ; Low Vine Climbing, Except Fuzzy Kiwifruit, Subgroup 13 **Analytical Chemistry and Residue Data PC Code:**  0063 2 9 **Decision No.:** 4 48205 **Petition No.:** 1E7864 **Risk Assessment Type:** NA **T XR No.:**  NA **MRID No s**  **.:** 4 84629 - 01 thru - 0 5 **FROM:** Sarah J. L ev y, Chemist

Risk Assessment Branch 1 (RAB1) Health Effects Division (HED **THROUGH:** George F. Kramer, Ph.D.,

RAB1 /HED ( 7509P **TO:** Barbara Madden, RM

Registration Division (RD; 7505P) **Executive Summary** Acequinocyl is a quinoline - ty pe miticide registered for use agricultural commodities (RACs) Acequinocyl is currentl y registered to Ary sta Lifescience North America Corporation as a 15% suspension - concentrate (SC) formulation. were a joint - review with Canada. Under PP # 1E7864 , the Interregional Research Project No.4 (IR Experiment Station of TX, CA, OR, PA, GA, NM, OK, TN, UT, MI, FL, and AZ for the establishment of tolerances for combined residues of the insecticide acequinocy l, 2 (acet yl oxy) - 3 - dodecyl - 1,4 - naphthalenedione, and its metabolite, 2 naphthoquinone (acequinocyl - OH) Summary of Analytical Chemistry and Residue Data

Page 1 of 30 **UNITED STA**

**TES ENVIRONMENTA L PROTECTION A** **GENCY**

**WA SHINGTON, D.C. 20460**  **OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION** 

etition for U se on Succulent So yb ean Vegetable; Succulent Shelled Beans; Cowpea Forage; Caneberry Subgroup 13 - 07A; Melon Subgroup 9A; Cucumber, Cherry ; Low - Growing Berry Subgroup 13 - 07G; and Small Fruit Vine Climbing, Except Fuzzy Kiwifruit, Subgroup 13 - 07F . **Summary of Analytical Chemistry and Residue Data**  **. DP Barcode:**  D 3 8 9 989 **Registration No. :**  66330 - 38 **Regulatory Action:** Section 3 **Case No.:** 7621 **CAS No.:** 57960 - 19 - 7 5 **40 CFR:**  § 180 .599 Chemist Risk Assessment Branch 1 (RAB1) Health Effects Division (HED ; 7509P ) George F. Kramer, Ph.D., Senior Chemist

7509P ) Barbara Madden, RM Team 05 Registration Division (RD; 7505P)

ty pe miticide registered for use in the U.S. on a number of raw agricultural commodities (RACs) for the control of various phyt ophagous mite species Acequinocyl is currentl y registered to Ary sta Lifescience North America Corporation as a 15%

concentrate (SC) formulation. Note that the cucumb er and caneberry field trial data review with Canada.

Interregional Research Project No.4 (IR - 4) , on behalf of the Agricultural TX, CA, OR, PA, GA, NM, OK, TN, UT, MI, FL, and AZ he establishment of tolerances for combined residues of the insecticide acequinocy l, 2 naphthalenedione, and its metabolite, 2 - dodecyl - 3 - hydrox y OH) , expressed as acequinocy l equivalents in/on the f DP#: 389989 **UNITED STA**

**TES ENVIRONMENTA L PROTECTION A** **GENCY OFFICE OF CHEMICAL SAFETY AND**  **POLLUTION PREVENTION**

Succulent So yb ean Vegetable; Succulent

07A; Melon Subgroup 07G; and Small Fruit **Summary of**

number of raw for the control of various phyt ophagous mite species . Acequinocyl is currentl y registered to Ary sta Lifescience North America Corporation as a 15% er and caneberry

field trial data , on behalf of the Agricultural TX, CA, OR, PA, GA, NM, OK, TN, UT, MI, FL, and AZ , is requesting he establishment of tolerances for combined residues of the insecticide acequinocy l, 2 - hydrox y - 1,4 - in/on the f

ollowingAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 2 of 30 commodities : Soy bean , vegetable , succulent ................................ ................................ .. 0. 25 ppm Lo w growing berry

subgroup 13 - 07G ................................ ...................... 0. 4 ppm Small fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit ........ 1.6 ppm Bean, succulent shelled ................................ ................................ ............. 0.15 ppm Cowpea, forage ................................ ................................ ......................... 9.0 ppm Caneberry

subgroup 13 - 07A ................................ ................................ ..... 4.5 ppm Melon subgroup 9A ................................ ................................ .................. 0.06 ppm Cucumber ................................ ................................ ................................ .. 0.15 ppm Cherry ................................ ................................ ................................ ....... 0.8 ppm No new data were submitted to support tolerances on succulent so ybean vegetables, low - growing berry 13 - 07G crop subgroup, or small fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit . The petitioner has requested that existing tolerances be expanded to their respective crops or crop groups. The end - use product proposed for use on the aforementioned proposed crops is KanemiteTM 15 SC Mit icide, a 1.25 lb/gal soluble concentrate ( SC ) formulation (alternate brand names: ShuttleTM 15 SC Miticide , and ShuttleTM O Miticide) . Up to two foliar , postemergence, ground applications are proposed for a maximum seasonal rate of 0.6 lb ai/A, with a 1 - da y preharvest interval (PHI) , except for succulent shelled beans and cherries for which a 7 - day PHI is proposed . T olerances are currentl y established under 40 CFR §180.599(a) for the combined residues of acequinocyl and its metabolite, 2 - dodecyl - 3 - hy droxy- 1,4 - naphthoquinone, expressed as acequinocyl equivalents , in/on a number of plant and livestock commodities. Tolerances for plant commodities range from 0.02 ppm (tree nuts and pistachios ) to 30 ppm (citrus oil), and tolerances for livestock commodities are established at 0.02 ppm in the fat and liver of cattle, goat, horse, and sheep . The nature of acequinocyl residues in apples, oranges, and eggplants is understood based on the previously

reviewed metabolism stud ies. In these crops , the metabolism of acequinocyl involves the loss of the acet yl oxy

moiety to form acequinocyl - OH, the opening of the quinone ring to form AKM - 18, and the subsequent degradation of the quinone ring to yield polar metabolites that degrade to phthalic acid. For purposes of risk assessment and the tolerance expression, HED has determined that the residues of concern in these commodities include parent and acequinocyl - OH (Memo, S. L ev y, 07 - JAN - 2004; DP# 297872). These data are considered su fficient for the currently requested crops. There are adequate residue anal yt ical methods for enforcing tolerances for acequinocyl residues of concern in/on the proposed/registered commodities. These methods include two high performance liquid chromatograph y methods with tandem mass - spectroscopy detection ( HP LC /MS/MS ) for determining residues in/on plant commodities (Morse Methods Meth - 135 and #Meth - 133, revision #3 ). For each of these methods, residues are extracted with hexane or acetonitrile (ACN)/water and cleaned up b y solvent partitioning, gel - permeation chromatograph y (GPC) and/or silica - gel solid - phase extraction (SPE). In the current field trials, residues of acequinocyl and acequinocyl - OH were determined using a modified version of Morse Method #Meth - 133, revision #3. The method limit of quantitation ( LOQ ; based on the lowest level ofAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 3 of 30 method validation ( LLM V ) ) for succulent shelled beans was 0.02 ppm for each analy te. The LOQ (based on the LLM V) for cantaloupe was 0.015 ppm for acequinocyl and 0.01 ppm for acequinocyl - OH. For cherries, cucumbers, and caneberries, the LOQ (based on the LLMV) was 0.01 for each analy

te. The method was adequately validated in conjunction with the anal ys is of fiel d trial samples. B ased on the potential dietary exposure of livestock to acequinocyl residues from the current and proposed uses, the dietary burden was re - evaluated. The re - evaluated dietary burden indicate s that the currently

established tolerance level of 0.02 ppm for residues in cattle, goat, horse, and sheep fat are appropriate. The established 0.02 - ppm residue level in cattle, goat, horse, and sheep liver is appropriate; however, the commodity definition should be changed to “ meat by products ” rather than “liver . ” With the establishment of the cattle, goat, horse, and sheep tolerance in meat by

products , RD should remove the tolerance (40 CFR §180.599) for liver. Furthermore, the meat byproducts tolerance of 0.02 ppm is adequate to co ver residues in the kidney of cattle, goats, horses, and sheep. **A revised Section F should be submitted.** T he data requirements for multiresidue (MRM) methods are fulfilled. Certain MRM protocols resulted in acceptable recoveries. The MRM testing data h ave been forwarded to the U.S. Food and Drug Administration ( FDA ) for further evaluation ( Memo, S. L ev y, 18 - FEB - 2004; DP # 298917 ). The submitted concurrent storage stability data for the subject crops are adequate to support the storage intervals and cond itions incurred by the field trial studies. IR - 4 submitted adequate field trial data in support of the proposed uses on succulent shelled beans, caneberry , cantaloupe, cherry , and cucumber. The field trials r eflect the proposed use patterns and an adequ ate number of field trials were conducted in major crop growing regions . The submitted field trial data reflect applications made without the use of a spray adjuvant. **A revised Section B should be submitted**  **prohibiting the addition of adjuvants to the spray solution**  **.** The Organization for Economic Co - operation and Development (OECD) maximum residue limit (MRL ) Calculator was used to determine the appropriate tolerance levels. The field trial data will suppo rt tolerances of: 0.30 ppm for residues in/on bean, succulent shelled; 6.0 ppm for residues in/on cowpea, forage; 18 ppm for residues in/on cowpea, hay ; 4.0 ppm for residues in/on caneberry

subgroup 13 - 07A; 0.15 ppm for residues in/on melon subgroup 9A; 1 .0 ppm for residues in/on cherry , tart; 0.50 ppm for residues in/on cherry , sweet ; and 0.15 ppm for residues in/on cucumber . **A revised Section F should be submitted.** No new data were submitted to support tolerances on succulent so ybean vegetables, low - growing berry 13 - 07G crop subgroup, or small fruit climbing vine subgroup 13 - 07F, except fuzzy kiwifruit. However, existing established tolerances in 40 CFR §180.599 should be extended to support these crop group tolerances. HED recommends that that the t olerance for residues in/on edible - podded bean s should be extended to soy bean, vegetable, succulent; the tolerance for residues in/on strawberries should be extended to the low - growing berry 13 - 07G subgroup ; and the tolerance for residues in/on grapes should be extended to small fruit vine climbing subgroup 13 - 07F, except fuzzy

kiwifruit (See Table 10 for details) . T here are no processed food/feed items associated with the proposed uses. Therefore, data requirements for this guideline are not relevant to this tolerance petition.Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 4 of 30 The proposed uses under this petition include the following ty picall y rotated crops: succulent soy beans, berries (subgroup 13 - 07G), succulent shelled beans, cowpea forage, cucumbers, and melons (subgr oup 9A) . An adequate c onfined rotational crop stud y was submitted and reviewed with a previous petition . The available data support a 30 - day plantbac k interval ( PBI ) for all non - labeled crops, which is reflected on the proposed label. **Regulatory Recommendations and Residue Chemistry Deficiencies** Pending submission of revised Sections B (see requirements under Directions for Use) and F ( see requirements under Proposed Tolerances ) there are no residue chemistry issues that would preclude granting a permanent registration for the requested uses of acequinocyl or establishment of tolerances for residues of acequinocyl and acequinocyl - OH as follows: “tolerances are established for residues of acequinocyl, including its metabolites and degradates, in or on the commodities in the table below. Compliance with the tolerance levels specified below is to be determined b y measuring only the sum of acequinocyl [ 2 - (acet yl oxy) - 3 - dodecyl - 1,4 - naphthalenedione] and its metabolite, 2 - dodecyl - 3 - hy droxy - 1,4 - naphthoquinone , calculated as the stoichiometric equivalent of acequinocyl, in or on the commodity .” Soy bean, vegetable, succulent

...... .................................... ............ . 0.25 ppm Low growing berry

subgroup 13 - 07G 1

............................................. . 0. 5 0 ppm Small fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit 1

... 1.6 ppm Bean, succulent shelled

................................................................................ . 0.30 ppm Cowpea, forage

........................................................................... 6.0 ppm Cowpea, hay

................................................................................. 18 ppm Caneberry

subgroup 13 - 07A ........................................................... . 4.0 ppm Melon subgroup 9 A ........................................................................ 0.15 ppm Cucumber

...................................................................................... 0 .15 ppm Cherry , tart ................................................................................. 1.0 ppm Cherry , sweet .............................................................................. 0.50 ppm Meat by products of cattle, goat, horse and sheep 1

........................... 0.02 ppm 1 Note to RD: With the establishment of these tolerances, the following tol erances should be removed: strawberry; grape; and liver of cattle, goat, horse, and sheep. A human - health risk assessment is forthcoming (D P# 389519) . 860.1200 Directions for Use  The submitted field trial data reflect applications made without the use of a spray adjuvant. A revised Section B should be submitted incorporating an adjuvant

– use restriction. 860.1550 Proposed Tolerances  A revised Section F is required to amend the tolerances as specified in Table 10 , as well as the tolerance expression (se e Conclusion s section under Table 10 for details ) .Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 5 of 30 **Background** The chemical structure and nomenclature of acequinocyl and its regulated metabolite are presented in Table 1, and the phy sicochemical properties of acequinocyl are presented in Table 2. **T able**  **1. Nom**  **enclature of Test Compound and Metabolites .** 

Com pound O

O C H 2 ( C H 2 ) 1 0 C H 3 O C O C H 3 Common name Acequinocyl Com pany experimental names TM - 41301 or AKD - 2023 IUPAC name 3 - dodecyl - 1,4 - dihydro - 1,4 - dioxo - 2 - naphthyl acetate CAS name 2 - (acetyloxy) - 3 - dodecyl - 1,4 - naphthalenedione CAS # 57960 - 19 - 7 End - use products/EP KanemiteTM 15 SC (1.25 lb ai/gal; EPA Reg. No. 66330 - 38) Com pound O

O C H 2 ( C H 2 ) 1 0 C H 3 O H Common name Acequinocyl - OH Com pany experimental names R1 IUPAC name 2 - dodecyl - 3 - hydroxy - 1,4 - napthoquinone CAS name 2 - dodecyl - 3 - hydroxy - 1,4 - naphthalenedione CAS # 57960 - 31 - 3 **T able**  **2. Physicochemical Properties**  **.** Parameter Value Reference (MRID) Melting point 59.6 C 45434906 pH 6.94 45434904 Density 1.13 g/cm 3 45434904 Water solubility (20°C) 6.69 μg/L 45434906 Solvent solubility (mg/L at 20°C) 1,2 - dichloroethane: >250 acetone: >250 ethyl acetate: >250 xylene: >250 methanol: 6.1 n - heptane: 36 n - octanol: 29.2 45434904

Vapor pressure at 25°C 1.69 x 10 - 9 pascals 45434905 Dissociation constant (pK a ) no measurable pK a 45434905 Octanol/water partition coefficient Log(K OW ) > 6.2 45434906 UV/visible absorption spectrum (  max, nm) neutral pH: 242, 248, 262, 270, 335 acid pH: 242, 248, 265, 270, 330 basic pH: 232, 245, 255, 275, 362 45434905Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 6 of 30 **860.1200 Directions for Use** The petitioner provided a draft label for Kanemite TM 15 SC Miticide (EPA Reg. No. 66330 - 38), a 1.25 lb/gal SC formulation of acequinocyl. This acequinocyl formulation is currentl y registered for use on several fruit and nut crops, fruiting vegetables, okra, edible - podded beans , and hops. The proposed uses are summarized below in Table 3. **Table 3**  **. Summary of**  **Proposed Directions for Use of**  **Acequinocyl .**  Applic. Timing, Ty pe, and Equip. Form

ulation [EPA Reg. No.] Applic.

Rate (lb ai/A) Max. No.

Applic. per Season Max. Seasonal

Applic. Rate (lb ai/A) PHI

(days) Use Directions and

Limitations 1 Bean, s ucculent shelled Postemergence, foliar ; G round only 1.25 lb/gal

SC [66330 - 38] 0.3 2 0.6 7 Minimum spray volume of 30 gal/A ; minimum RTI of 14 day s. Do not allow grazing on cow

pea forage within 7 days of last treatment. Caneberry subgroup 13 - 07A Postemergence, foliar ; Ground only 1.25 lb/gal

SC [66330 - 38] 0.3 2 0.6 1 Minimum spray volume of 5 0 gal/A; minimum RTI of 21 days. Melon subgroup 9A Postemergence, foliar ; Ground only 1.25 lb/gal

SC [66330 - 38] 0.3 2 0.6 1 Minimum spray volume of 30 gal/A; minimum RTI of 21 days . Cucumber Postemergence, foliar ; Ground only 1.25 lb/gal

SC [66330 - 38] 0.3 2 0.6 1 Minimum spray volume of 100 gal/A; minimum RTI of 21 days. The use of an adjuvant or surfactant is prohibited. Cherry Postemergence, foliar; Ground only 1.25 lb/gal

SC [66330 - 38] 0.3 2 0.6 7 Minimum spray volume of 4 0 gal/A; for optimal control, use a minimum of 150 gal/A ; minimum RTI of 14 days. Do not use in alternate row middle applications. 1 RTI = retreatment interval. General label restrictions include: (i) do not appl y through an y typ e of irrigation sy stem; (ii) do not apply by air; (iii) do not apply

within 75 feet of aquatic areas; and (iv) not for indoor residential use. The product may be combined with most commo nly used pesticides with the exception of strongl y alkaline material, which can reduce activity; combination with Phos - ethyl (foset yl ) is prohibited. Broad - spectrum insect control may be obtained by

tank - mixing the SC acequinocyl product with other mitici de/insecticide products; a spray mix compatibility and phy totoxicity trial under local conditions should first be conducted.Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 7 of 30 R otational crop restrictions are as follows: f ood and feed crops not listed on the proposed label must not be planted within 30 day s of the last Kanemite TM 15 SC application. Conclusions : The proposed use directions are adequate to allow for evaluation of the submitted residue data relative to the proposed uses. The proposed uses are supported by the submitted field trial data . Furthermore, the submitted field trial data reflect applications made without the use of a spray adjuvant. **A revised Section B should be submitted incorporating an adjuvant –**  **use restriction.**  An adequate confined rotational crop study was previously

submitted , reflecting the maximum proposed use pattern for rotated crops . The available data support a 30 - day PBI for all non labeled crops, which is reflected on the proposed label. **860.1300 Nature of the Residue –**  **Plants /Livestock**  The qualitative nature of acequinocyl residues in fruit and nut crops is understood based on the available apple, orange, and eggplant metabolism studies ( Memo, S. L ev y, 10 - MAR - 2004; DP# 284757). Residues in/on both the fruit and foliage were characteriz ed in these metabolism studies . The data indicate that the metabolism of acequinocyl in these crops involves the loss of the acet yl oxy

moiet y to form acequinocyl - OH, opening of the quinone ring to form AKM - 18, and subsequent degradation of the quinone rin g to yield polar metabolites that degrade to phthalic acid. Based on the structural similarity of acequinocyl - OH to parent and the presence of acequinocyl OH at quantifiable levels in crops, the HED Metabolism Assessment Review Committee (MARC) concluded that parent and acequinocyl - OH are the residues of concern for risk assessment and tolerance e xpression in apples, oranges, and eggplant commodities (Memo, S. Lev y, 07 - JAN - 2004; DP# 297872) . For purposes of this petition, HED is wi lling to translate these results to caneberries, cantaloupe, cherry , and cucumber as these are fruit crops and succulent shelled beans is a low - consumption food item. However, if in the future , the petitioner requests new uses where apple, orange, and eggplant metabolism data cannot be translated, then HED may

ask for additional plant metabolism data. The qualitative nature of acequinocyl residues in ruminants is understood based on an adequate goat metabolism study

(Memo, S. L ev y, 10 - MAR - 2004; DP# 284757 ). The metabolism of acequinocyl in goats appears to involve the loss of the acetyloxyl moiet y t o form acequinocyl OH and partial cleavage of the dodecyl side chain to form AKM - 15. Opening and degradation of the quinone ring was also evidenced b y the pre sence of AKM - 18 and phthalic acid. The MARC concluded that for tolerance expression, the residues of concern in livestock commodities include parent and acequinocyl - OH. For risk assessment purposes, the residues of concern in livestock commodities are parent and acequinocyl - OH (for liver and kidney , metabolite AKM - 15 is to be included as well (Memo, S. L ev y, et al ., 07 - JAN - 2004; DP # 297872 ). Metabolism data on poultry are not required at the present time as none of the proposed or existing uses of acequinocyl are on crops having regulated poultry feedstuffs.Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 8 of 30 **860.1340 Residue Analytical Methods** Enforcement methods: Adequate anal yt ical methods are available for enforcing acequinocyl tolerances in/on the proposed/registered plant commodities ( Memo , D. Soderberg 23 - MAY 2006; DP# 321753 ; Memo, S. L ev y, 25 - JUN - 2008; DP#s 351336 & 353313) . These methods include two HP LC /MS/MS methods for determining acequinocyl and acequinocyl - OH residues in fruit crops (Morse Method #Meth - 133, revision #3) and nut cr ops (Morse Method #Meth 135). The three methods were forwarded b y HED to Biological and Economical Anal ys is Division ’s ( BEAD ’s ) Analy tical Chemistry

Branch ( ACB ) for a petition method validation ( PMV ) . In lieu of a PMV, ACB completed a desk audit (Memo, P. Golden, 04 - APR - 2006; ACB# B04 - 13) in which ACB recommended that the petitioner revise methods # Meth - 133, Revision #3 (including the modification for orange oil), # Meth - 135, and # Meth - 139 Revision #2 to address deficiencies. The petitioner adequately addressed the modifications requested b y ACB ( Memo, P. Golden, 04 - APR - 2006; ACB# B04 - 13 ). Methods #Meth - 135, Revision #3, #Meth - 133, Revision #4, and #Meth - 139, Revision #3 are suitable enforcement m ethods for the subject crop and livestock matrices and no additional data concerning these methods are necessary (Memo, S. Levy , 25 - AUG - 2010; DP# 380302). Data -gathering methods: Samples of crop commodities that were collected from the magnitude of the r esidue studies were anal yz ed for residues of acequinocyl and acequinocyl - OH using modifications of the enforcement methods. S amples from all submitted field trials were anal yz ed using a modified version of Method Meth - 133 . A summary is given below. Brie fl y, 10 - g samples were extracted with ACN. Extra water was added to insure that a minimum amount of water was available for adequate anal yt e extraction. Following extraction, the water was salted out, and an aliquot of the resulting ACN phase was extract ed with hexane to separate the analy

tes from the undesirable ACN - soluble co - extracted materials. A final purification, incorporating si lica SPE cartridge cleanup, was conducted. The purified extract was concentrated and then submitted to HP LC /MS/MS analy sis. The LLMV for succulent shelled beans was 0.02 ppm for each anal yt e. The LLMV for cantaloupe was 0.015 ppm for acequinocyl and 0.01 ppm for acequinocyl - OH. For cherries , cucumbers, and caneberries , the LLMV was 0.01 for each analy te. Conclusions : An a dequate HP LC /MS/MS method (Morse Method #Meth - 133, revision #3) is available for enforcing the proposed tolerance s , and the residue data from the field trials were determined using an adequate modification of this . The method LOQ (based on the LLMV ) for succulent shelled beans was 0.02 ppm for each analy te. The LOQ (based on the LLMV) for cantaloupe was 0.015 ppm for acequinocyl and 0.01 ppm for acequinocyl - OH. For cherries, cucumbers, and caneberries, the LOQ (based on the LL MV) was 0.01 for each anal yt e. **860.1360 Multiresidue Methods** Acceptable MRM method testing data were submitted for acequinocyl and acequinocyl - OH, and these data were forwarded to the U.S. FDA for further evaluation ( Memo, S. L ev y, 18 - FEB 2004; DP # 298917). Acequinocyl was adequately recovered from fortified apples using Method 302, E1 + C1 with GC/ electron - capture detector ( ECD ) , but was not recovered using Method 303 or 304 due to poor recoveries from the Florisil cleanup. Acequinocyl - OH was completel y recovered from fortified apples using Method 402, E1 , or E2 + C1 with GC/ECD, but was notAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 9 of 30 recovered using Methods 302, 303, or 304. **860.1380 Storage Stability** A summary

of storage stability data are given below for each newly proposed crop. S ucculent beans Samples of succulent bean seeds without pods were stored frozen for up to 824 day s prior to anal ys is and 802 day s for bean plants with pods. The freezer storage stability of acequinocyl and acequinocyl - OH was assessed concurrentl y in this s tudy , with samples spiked at 0.1 ppm. Results from the concurrent storage stability study indicate that residues of acequinocyl and acequinocyl - OH are stable for up to 781 day s in/on bean plants with pods (seed removed) and that residues of acequinocyl - OH are stable for up to 757 day s in/on succulent bean seeds without pods and that acequinocyl residues decline ~50% during this interval in/on succulent bean seeds . Residues were adjusted to account for the decline during storage. Caneberry Treated canebe rry

samples were stored frozen for up to 719 day s prior to extraction. All samples were anal yz ed within 1 day of extraction. The freezer storage stabilit y of acequinocyl and acequinocyl - OH residues in/on caneberries was assessed concurrentl y in this study, with samples spiked at 0.1 ppm. The mean corrected residue recoveries (corrected for concurrent recoveries from freshl y spiked samples) of acequinocyl and acequinocyl in samples stored frozen for 685 day s were 84% (n=3, SD=5 %) and 80% (n=3, SD=3%), re spectivel y. As such, there are no concerns with the stability of acequinocyl and acequinocyl - OH over time in/on caneberry samples from this study . Cantaloupe Treated cantaloupe samples were stored frozen for up to 751 day s prior to extraction. Cantaloup e fruit storage stability samples were fortified with acequinocyl and acequinocyl - OH, each at 0.1 ppm. Storage stability samples were analyzed 712 day s after fortification. Storage stability cantaloupe fruit samples averaged 66 ± 2% (n=3) for acequinocyl and 77 ± 3% (n=3) for acequinocyl - OH. Concurrent storage stability recovery spikes averaged 98 ± 3% for acequinocyl and 84 ± 4% for acequinocyl - OH. Results from the concurrent storage stability study indicate that residues of acequinocyl and acequinocyl - OH are stable for up to 712 day s in/on cantaloupe and that acequinocyl residues decline to ~67% during this interval. Residue s were adjusted to account for the decline during storage. Cherry Treated cherry

samples were stored frozen for up to 

### 874 days (~29 months). Results from the concurrent storage stability study indicate that residues of acequinocyl and acequinocyl - OH are stable for up to 874 day s in/on cherries. The mean corrected residue recoveries (corrected for concurrent recoveries from freshly spiked samples) of acequinocyl - OH in samples stored frozen for 874 day s were 80% (n=3, SD=7). There are no concerns with the stability of acequinocyl over time in/on cucumber samples from this study. Cucumbers Treated cucumber samples were stored frozen for up to 799 day s prior to extraction . The freezer storage stability

of acequinocyl and acequinocyl - OH residues in/on cuc umbers was assessed concurrently

in this study, with samples spiked at 0.1 ppm. The mean corrected residueAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 10 of 30 recoveries (corrected for concurrent recoveries from freshly spiked samples) of acequinocyl and acequinocyl - OH in samples stored frozen for 741 day s were 59% (n=3, SD=6 %) and 116% (n=3, SD=5%), respectively . There are no concerns with the stability of acequinocyl - OH over time in/on cucumber samples from this study . However, residues of acequinocyl in/on cucumbers declined significantly over a perio d of 741 day s. As such, residues of acequinocyl in/on treated cucumber samples were corrected for storage dissipation. The storage duration s and conditions of samples from the crop field trial studie s submitted to support this petition are presented in T able 4 . **Table 4**  **. Summary of Storage Conditions and**  **Duration s of Sam**  **ples from C**  **rop F**  **ield T**  **rial Studies.**  Matrix Storage

Tem perature (°C) Actual Storage

Duration Interval of Demonstrated Storage Stability Succulent bean seeds without pods < - 20 8 02 days ( 2 6.4 months) Results from the concurrent storage stability study indicate that

residues of acequinocyl - OH are stable for up to 757 days and that acequinocyl residues decline ~50% during this interval. Succulent bean plants with pods 82 4 days (26.4 months) Results from the concurrent storage stability study indicate that

residues of acequinocyl and acequinocyl - OH are stable for up to 781 days. Caneberries - 20 719 days ( 23.6 months) Results from the concurrent storage stability study indicate that

residues of acequinocyl and acequinocyl - OH were stable for up to 685 days. Cantaloupe < - 20 751days ( ≤26.4 months) Results from the concurrent storage stability study indicate that

residues of acequinocyl - OH are stable for up to 712 days and that acequinocyl residues decline to ~67% during this interval. Cherry < - 20 874 days ( ≤28.7 months) Results from the concurrent storage stability study conducted on

cherries indicate that residues are stable for up to 874 days. Cucumbers - 20 799 Corrected recoveries of acequinocyl residues from cucumbers stored frozen for up to 741 days ranged from 53

– 64% (mean ± SD = 59 ± 6%) indicating dissipation of residues. Results from the concurrent storage stability study indicate that residues of acequinocyl - OH we re stable for up to 741 days. Conclusions : The submitted concurrent storage stability data for the above - listed crops is adequate to support the storage intervals and conditions incurred b y the field trial studies. **860.1480 Meat, Milk, Poultry, and Eggs**  Based on the available goat and poultry metabolism data and the previously calculated dietary exposures of livestock to acequinocyl residues, HED previously set livestock commodities on cattle, goat, horse, and sheep fat and liver at 0.02 ppm ( for mo re details, see: S . L ev y, 10 - MAR 2004; D284757; and 05 - SEP - 2007; D330439 ) . Exposure of poultry and hog to acequinocyl is not expected, as there are no established or proposed tolerances on poultry or hog feedstuffs. The maximum reasonable dietary burden s (M RDBs) of livestock to acequinocyl residues were previously

calculated to be 0.72 ppm for beef cattle and 0.47 ppm for dairy cattle . As cowpea forage and hay are significant feedstuff for livestock ( dairy cattle only) , the MRDB of acequinocyl was reca lculated for this petition using the Agency ’s most recent guidance on constructing reasonabl y balanced livestock diets ( Science Advisory Council for Chemistry (ChemSAC) memo, 30 - JUN - 20 08) . The beef cattle MRDB s did not need to be recalculated asAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 11 of 30 cowpea fo rage and hay

are not beef feed items . The recalculated MRDB for dairy cattle is 1. 73 ppm (Table 5). **Table 5. Calculation of Dietary Burdens of Acequinocyl**  **Residues for Livestock.** Feedstuff Dietary Com ponent % Dry

Matter 1 % Diet 1 Residues (ppm) 2 Dietary Contribution

(ppm) 3 **Dairy Cattle** Cow pea , forage R 3 0 20 2.59 1.7 3 Apple, wet pomace CC 40 10 0.01 0. 00 25 TO TA L BURDEN **100 1.**  **73** 1 Potential dietary burdens for livestock were determined using recent Agency guidance on calculating reasonably balanced diets for livestock (memo, 30 - JUN - 2008). 2 The tolerance level for wet apple pomace w as used and the HAFT w as used for cow pea forage . 3 Contribution = ([tolerance (or HAFT) /% DM] X % diet) for dairy cattle. Dairy cattle feeding study

(previousl y reviewed: 45651610.de2) In a ruminant feeding study , three groups of lactating dairy cows (4/group) were dosed orall y twice a day

for 28 consecutive day s with gelatin capsules containing acequinocyl at target doses equivalent to 5, 15, and 50 ppm (5 DG, 1 5 DG, and 50 DG) in the feed on a dry weight basis. Based on the average daily dietary intake, the actual dose levels were equivalent to 4.9, 14.9, and 48.6 ppm of acequinocyl in the diet. The LOQ for combined acequinocyl residues is 0.02 ppm in milk and all tissues. The limits of detection ( LODs ) for both anal yt es were not reported. However, the LODs for each analy te have been estimated to be 0.001 ppm in milk, 0.002 ppm in muscle and fat , and 0.005 ppm in liver and kidney s. With the exception of two fat samples, residues of acequinocyl were <LOD in all samples of milk and tissues from all three dose groups. Acequinocyl residues above the LOD were found in samples of milk and tissues fro m each dose group, with the exceptions of kidney and muscle samples from the low dose group, which had residues <LOD. In milk, the combined residues of acequinocyl and acequinocyl - OH (expressed in parent equivalents) were <0.002 - 0.003 ppm for the 5 ppm DG , <0.001 - <0.005 ppm for the 15 ppm DG, and <0.003 - 0.013 ppm for the 50 ppm DG. Residues appeared to plateau by Study Day 4. Combined residues were <LOQ in all milk samples. Inliver, combined residues were <0.014 - <0.032 ppm for the 5 ppm DG, <0.022 - <0.0 37 ppm for the 15 ppm DG, and <0.059 - <0.089 ppm for the 50 ppm DG . In kidney s, combined residues were <0.01 ppm for the 5 ppm DG, <0.010 - <0.014 ppm for the 15 ppm DG, and <0.019 - <0.040 ppm for the 50 ppm DG. In muscle, combined residues were <0.004 ppm for the 5 ppm DG, <0.004 - <0.006 ppm for the 15 ppm DG, and <0.007 - <0.008 ppm for the 50 ppm DG. In fat, combined residues were <0.006 - <0.013 ppm for the 5 ppm DG, <0.018 - <0.030 ppm for the 15 ppm DG, and <0.044 - <0.113 ppm for the 50 ppm DG. For the 5, 15 and 50 DGs, the average combined residues were respectivel y <0.002, <0.003, and <0.006 ppm in milk; <0.023, <0.030, and <0.070 ppm in liver; <0.010, <0.012, and <0.034 ppm in kidney s; <0.004, <0.005, and <0.007 ppm in muscle; and <0.009, <0.022, and <0.07 5 ppm fat. The combined acequinocyl residues increased linearly with increased feeding level in liver and fat, but there was no linear relationship between feeding level and residues in the other tissues and milk. Considering onl y the LOQ (0.01 ppm) for the two anal yt es, the combined residues were <LOQAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 12 of 30 (<0.02 ppm) in milk and muscle samples from all three dose groups, the kidney samples from the 5 and 15 ppm DG and fat samples from the 5 DG . Residues above the combined LO Q were found in liver at all thre e dose groups , fat samples from the 15 and 50 DG, and in kidney from the 50 ppm DG. **T able**  **6 .**  **Summary of Milk and Tissue Residue Data from Ruminant Feeding Study with Acequinocyl.**  Matrix Feeding

Level (ppm) Study Days 1 Combined Acequinocyl Residue Levels (ppm) 2 n Min. Max. Mean Std. Dev. Milk 5 4, 8, 12, 16, 20, 24, 28 28 <0.002 <0.003 <0.002 0.0003

15 4, 8, 12, 16, 20, 24, 28 28 <0.002 <0.005 <0.003 0.0009 50 4, 8, 12, 16, 20, 24, 28 28 <0.002 0.013 <0.006 0.0021 Liver 5 28 4 <0.014 <0.032 < 0.023 0.007

15 28 4 <0.022 <0.037 <0.030 0.006 50 28 4 <0.059 <0.089 <0.070 0.014 Kidney 5 28 4 <0.010 <0.010 <0.010 0

15 28 4 <0.010 <0.014 <0.012 0.002 50 28 4 <0.019 <0.040 <0.034 0.009 Muscle 5 28 4 <0.004 <0.004 <0.004 0

15 28 4 <0.004 < 0.006 <0.005 0.001 50 28 4 <0.007 <0.008 <0.007 0.0005 Fat 5 28 3 <0.006 <0.013 <0.009 0.004

15 28 4 <0.018 0.030 <0.022 0.005 50 28 4 <0.044 <0.113 <0.075 0.033 1 As residues in milk appeared to plateau by Study Day 4, collected samples from Days 4 - 28 were used to calculate average residues. Tissue samples were collected from animal sacrificed within 24 hours of receiving the final dose. 2 The LOQ for combined acequinocyl residues (expressed in parent equivalents) is 0.02 ppm in all matrices; the

combined LOD was estimated by the reviewer to be 0.004 ppm in fat and muscle, and 0.010 ppm in liver and ki dney. Values proceeded by a “<” indicate that residues of one or more of the analytes were below the LOD. Expected secondary

residues in meat and m ilk To determine the need for tolerances for acequinocyl residues in tissues, the anticipated secondary residues in cattle matrices were estimated using transfer coefficient factors calculated from the maximum residues of acequinocyl observed in the rumin ant feeding stud y . Onl y the tissue matrice s with detectable residues (>0.02 ppm) are shown below in Table 7 . The transfer coefficients (calculated as residue - level - to - feed ratios) are also show in Table 7 . The transfer coefficient for each matrix was th en used to calculate the expected secondary residues by multiplying the transfer coefficient by the calculated dietary

burden (Table 8 ). **Table 7**  **. Residue -**  **Level -**  **to -**  **Feed Ratios (Transfer Coefficients) in Dairy Tissues.** Matrix Maximum Residue

(ppm) Feeding Level, ppm Transfer Coefficient **1** Liver <0.032 5 <0.0064 Ave. = < 0.00 3

<0.037 15 <0.002 <0.089 50 <0.0018 Kidney <0.040 50 <0.0008 Fat 0.0 30 15 0.002 Ave. = < 0. 002

< 0. 113 50 <0.0023 1 Calculated from the maximum residues divided by the dose level.Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 13 of 30 **Table 8.**  **Expected Secondary Residues in Meat.** Matrix Dietary

burden (ppm) Secondary

Residues (ppm) 1 Established

Tolerance (ppm) HED Recommended Tolerance (ppm) Notes to RD Liver 1 .73 0.005 2 0.02 2

-- A new tolerance of 0.02 ppm in/on cattle, goat, horse, and sheep meat byproducts should be established to cover residues in liver and kidney. Kidney 0.0014 - 0.02 1 Fat 0.00 35 0.02 0.02 The currently - established tolerance is adequate. 1 Calculated from dietary burden x average transfer coefficient from Table 7 . 2 The established liver tolerance should be removed in 40 CFR §180.599 wi th the establishment of a new meat byproducts tolerance. Conclusions : The feeding stud y data indicate that the currently established tolerance level at 0.02 ppm for residues in cattle, goat, horse, and sheep fat are appropriate . The established 0.02 - ppm residue level in cattle, goat, horse, and sheep liver are appropriat e; however, the commodity definition should be changed to “meat by products” rather than “liver . ” With the establishment of the cattle, goat, horse, and sheep tolerance in meat by products , RD should remove the tolerance for liver. Furthermore, the meat by products tolerance of 0 .0 2 ppm is adequate to cover residues in the kidney

of cattle, goats, horses, and sheep. **A revised Section F should be submitted. 860.1500 Crop Field Trials**  DER References: 48462901.der (bean, succulent shelled)

48462902.der (caneberry) 48462903.der (cantaloupe) 48462904 .de r ( cherry) 48462905 .der ( cucumber ) No new data were submitted to support tolerances on succulent so ybean vegetables, low - growing berry 13 - 07G crop subgroup, or small fruit vine climbing subgroup 13 - 0 7F, except fuzzy kiwifruit. A summary

of the data submitted for the proposed new crops can be found below. Succulent soy

bean vegetable No new data were submitted for this crop ; h owever, an edible - podded bean tolerance is established in 40 CFR §180.599 at 0.25 ppm. IR - 4 has requested that this tolerance be extended to support a succulent soy bean vegetable tolerance. Conclusion: HED recommends that a tolerance at 0.25 ppm in/on edible - podded beans should be extended to soy

bean, vegetable, succulent . Low - growing berry

subgroup 13 - 07G No new data were submitted for this crop subgroup ; h owever, a strawberry tolerance is established in 40 CFR §180.599 at 0.40 ppm. I R - 4 has requested that this tolerance be extended to support a low - growing berry 13 - 07G subgroup crop tolerance . Conclusions: HED recommends that the established strawberry tolerance be extended to the low - growing berry

13 - 07G crop subgroup . The OECD Calculator shows that the data support a tolerance of 0.40 ppm for the low - gr owing berry sub group 13 - 07G (which includes strawberries) . Canada has a MRLof 0.5 ppm in/on strawberries. In order to harmonize withAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 14 of 30 Canada, HED recommends establishing a tolerance of 0.50 ppm for this subgroup. **A revised Section F should be submitted.**  With establis hment of this crop subgroup, the strawberry tolerance in 40 CFR §180.599 should be removed. Small fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit No new data were submitted for this crop subgroup ; h owever, a grape tolerance is established in 40 CFR §180.599 at 1.6 ppm. IR - 4 has requested that this tolerance be extended to support small fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit tolerance. Conclusions: HED recommends that the established grape tolerance be extended to small fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit at 1.6 ppm. With establishment of this crop subgroup, the grape tolerance in 40 CFR §180.599 should be removed. IR - 4 submitted field trial data in support of the proposed uses on succulent shell ed beans , caneberry , cantaloupe, cherry , and cucumber . The results of the field trials are summarized in Table 9 . **Table 9**  **. Summary of Residue Data from** **Crop Field Trials with Acequinocyl**  **.** Commodity Total Applic. Rate (lb ai/A) PHI

(days) Total Acequinocyl and Acequinocyl - OH Residues (ppm)

n Min. Max. LAFT 1 HAFT 1 Median Mean Std. Dev. **BEAN, SUCCULENT SHELLED (proposed use =**  **0.6 lb ai/A**  **total application rate ,**  **7 -**  **day PHI)** 2 Bean seeds without pods 0.610 - 0.911 6 - 8 14 <0.0 6 <0.1 25 <0.06 < 0.173 0.0 6 0.08 1 0.0 4 Bean plants with pods 0.610 - 0.911 6 - 9 14 <0. 34 3.03 <0.345 2.59 1.69 1.58 0. 96 **CANEBERRY , SUBGROUP 13**  **- 07A**  **(proposed use = 0.6 lb ai/A total application rate, 1**  **- day PHI)**  3 Raspberry 0.584 - 0.606 1 5 <0.13 2.28 <0.28 1.91 0.801 0.958 0.711 Blackberry 0.607 - 0.612 1 2 0.564 0.785 0.606 0.675 0.606 0.640 --

**ME LON SUBGROUP 9A**  **(proposed use = 0.6 lb ai/A total application rate, 1**  **- day PHI)**  4 Cantaloupe 0.602 - 0.833 1 16 <0.025 <0.122 <0.027 <0.089 <0.039 0.040 0.01

**CHERRY (proposed use = 0.6 lb ai/A total application rate, 7**  **- day PHI)**  5 Cherry, tart 0.6042 - 0.6211 6 - 7 12 <0.160 <0.481 <0.170 <0.461 0.290 0.319 0.115 Cherry, sweet 0.5836 - 0.6204 6 - 7 12 <0.060 <0.216 <0.062 <0.216 0.135 0.140 0.054

**CUCUMBER (proposed use = 0.6 lb ai/A total application rate, 1**  **- day PHI)**  6 Cucumber, field 0.567 - 0.625 1 8 <0.02 <0.069 <0.02 <0.061 0.044 0.042 0.018 Cucumber, greenhouse 0.597 - 0.604 1 2 <0.029 <0.093 <0.030 <0.069 0.037 0.049 - 1 LAF T = lowest - average - field - trial; HAFT = highest - average - field - trial. 2 Total residues = Acequinocyl (corrected for storage decline where app licable) + Acequinocyl - OH. LOQ = 0.02 ppm for each analyte . 3 Total residues = Acequinocyl + Acequinocyl - OH (corrected for concurrent recovery where applicable). LOQ = 0.01 ppm for each analyte. 4 Total residues = Acequinocyl ( corrected for storage decline where applicable ) + Acequinocyl - OH. LOQ = 0.015 ppm for acequinocyl; 0.01 for acequinocyl - OH. 5 Total residues = Acequinocyl + Acequinocyl - OH . LOQ = 0.01 ppm for each analyte. 6 Total residues = Acequinocyl ( corrected for storage dissipation ) + Acequinocyl - OH. LOQ = 0.01 ppm for each analyte.Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 15 of 30 Bean, Succulent Shelled IR - 4 has submitted field trial data for acequinocyl on succulent shelled beans and bean plant with the pod (seed removed). Seven bean field trials were conducted in North American Free Trade Agreement (NAFTA) Growing Zone 10 (CA; 2 tria ls), Zone 2 (GA, 2 trials), Zone 5 (OH; 1 trial), Zone 2 (SC; 1 trial), and Zone 11 (WA; 1 trial) during the 2006 growing season. At each site, a 1.25 lb/gal SC formulation of acequinocyl was applied to beans as two foliar directed applications at rates o f 0.302 - 0.378 lb ai/A/application, with a 14 - to 15 - day RTI, for a total rate of 0.610 - 0.680 lb ai/A (except in the case of one CA trial where a third application was made for a total rate of 0.911 lb ai/A). Applications were made using ground equipment in spray volumes of 36 - 72 gal/A, without a spray adjuvant. Applications were timed so that commerciall y mature succulent bean seed without pods could be collected at a 6 - to 8 - day PHI and commercially

mature bean plants with pods (seed removed) could be c ollected at a 6 - to 9 - day PHI . A residue - decline study was not conducted. Total combined residues of acequinocyl (corrected for decline during storage) and acequinocyl OH in/on bean seeds without pods ranged from <0.06 - <0.125 ppm, harvested 6 - 8 day s foll owing foliar applications of the SC formulation for a total rate of 0.610 - 0.911 lb ai/A. Total combined residues of acequinocyl and acequinocyl - OH in/on bean plants with pods ranged from <0.34 - 3.03 ppm, harvested 6 - 9 day s following foliar applications of the SC formulation for a total rate of 0.610 - 0.911 lb ai/A (see Table 9 ) . Conclusions : The submitted succulent bean field trial data are acceptable and reflect the use of the acequinocyl 1.25 lb/gal SC formulation as two foliar applications for a total rate of 0.610 - 0.911 lb ai/A (trial 06 - CA*28 reflected three applications of approximately 0.303 lb ai/A, for a total of 0.909 lb ai/A), with a 6 - to 8 - day PHI. Total combined residues of acequinocyl (corrected for decline during storage) and acequinocyl OH in/on bean seeds without pods ranged from <0.06 - <0.125 ppm, harvested 6 - 8 day s following foliar applications of the SC formulation for a total rate of 0.610 - 0.911 lb ai/A. Total combined residues of acequinocyl and acequinocyl - OH in/on bean plants wi th pods ranged from <0.34 - 3.03 ppm, harvested 6 - 9 day s following foliar applications of the SC formulation for a total rate of 0.610 - 0.911 lb ai/A. The residue data for bean seeds without pods and bean plants with pods were entered separately into the O ECD MRLCalculator. Based on the results of these trials, the appropriate tolerance for residues of acequinocyl and acequinocyl - OH in/on bean, succulent shelled is 0. 30 ppm and cowpea, forage is 6.0 ppm . Ty picall y, forage is harvested before the plant ha s bloomed. However, the label does not specify at what plant stage the product can be applied. The plants were harvested and analyzed after the plants were blooming/fruiting. Therefore, HED deems it necessary to establish a tolerance on cowpea, hay as w ell. There is ty

pically a 3 - fold drying factor between forage and hay . As HED is recommending for a tolerance for residues in/ on cowpea, forage at 6.0 ppm, a tolerance for residues of acequinocyl and acequinocyl - OH in/on cowpea, hay

at 18 ppm should be e stablished. **A revised Section F should be**  **submitted .**  Caneberries The IR - 4 has submitted field trial data for acequinocyl in/on caneberries. Five supervised cropAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 16 of 30 field trials for acequinocyl in/on raspberries and two supervised crop field trials for ace quinocyl in/on blackberries were conducted in the U.S. and Canada during the 2007 growing season encompassing NAFTA Growing Zones 2 (1 raspberry trial in NJ), 5A (1 raspberry trial in MI), 10 (1 blackberry

trial in CA), and 12 (1 raspberry trial in BC, 1 r aspberry trial in OR, and 1 blackberry

trial in OR). At each trial site, acequinocyl (formulated as Kanemite TM 15 SC) was applied to caneberries as 2 foliar directed or broadcast applications at rates of 0.289 - 0.313 pounds ai /A, with RTI s of 15 - 23 day s, fo r a total seasonal application rate of 0.584 - 0.612 pounds ai /A. Applications were made in spray volumes ranging from 51 - 111 gallons/A. No adjuvants were used in an y of the spray mixtures. Commercially mature berries were harvested at a PHI of 1 day . At the QC03 trial site, mature berries were harvested at additional PHI s of 0, 4, 6, and 11 day s to assess residue - decline behavior. Total residues of acequinocyl and acequinocyl - OH (corrected for concurrent recoveries) in/on raspberries treated at total rates of 0.584 - 0.606 pounds ai /A/season and harvested at a PHI of 1 day ranged from <0.13 - 2.28 ppm. Total residues of acequinocyl and acequinocyl - OH (corrected for concurrent recoveries) in/on blackberries treated at total rates of 0.607 - 0.612 pounds ai /A/season and harvested at a PHI of 1 day ranged from 0.564 - 0.785 ppm (Table 9) . Residue decline data indicated that total residues of acequinocyl and acequinocyl - OH in/on raspberries declined with increasing PHI s. Conclusions : The supervised crop field trials for acequinocyl in/on caneberries are considered scientifically

valid. Kanemite TM 15 SC was applied to raspberries and blackberries as 2 foliar applications at rates total rates of 0.584 - 0.612 pounds ai /A/season and harvested commercially mature caneberries at a PHI of 1 day . Total residues of acequinocyl and acequinocyl - OH (corrected for concurrent recoveries) in/on raspberries ranged from <0.13 - 2.28 ppm. Total residues of acequinocyl and acequinocyl - OH (corrected for concurrent recoveries) in/on blackberries ranged from 0.564 - 0.785 ppm. Residue - decline data in/on raspberries indicated that total residues of acequinocyl and acequinocyl - OH in/on raspberries declined with increasing PHI s. The residue data for raspberries were entered into the OECD MRLCalculator (although field trial data were submitted for blackberries, there were not enough data to input into the OECD calculator) . Note that for caneberries, crop subgroup 13 - 07A, the representative crop is either raspberry **or** blackberry . A minimum of five trials is required on an y one blackberry or an y one raspberry if a tolerance is sought on “ caneberries. ” Based on the results of these trials, the appropriate tolerance for residues of acequinocyl and acequinocyl - OH in/on “caneberry subgroup 13 - 07A” is 4.0 ppm. **A revised Section F is requested.** Cantaloupe IR - 4 has submitted field trial data for acequinocyl on cantaloupe. Six cantaloupe trials were conducted in NAFTA Growing Zone 2 (SC; 1 trial), Zone 5 (OH, 1 trial), Zone 6 (TX; 1 trial), and Zone 10 (NM, AZ, and CA; 3 trials) during the 2007 growing season. At the trial sites, a 1.25 lb/gal SC formulation of acequinocyl was applied to cantaloupe as two broadcast foliar application at rates of 0.299 - 0.307 lb ai/A/application for a total rate of 0.600 - 0.609 lb ai/A (except in the case of one OH trial where a third application was made for a total rate of 0.833 lb ai/A). Applications were made at 20 - to 21 - day RTIs except for the OH trial, where the second application was made at a 15 - day RT I. Applications were made using groundAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 17 of 30 equipment in spray

volumes of 33 - 49 gal/A, without a spray adjuvant. Applications were timed so that the cantaloupe could be collected at a 1 - day PHI . A residue - decline study was conducted at the NM trial where sampling was conducted at a 0 - , 1 - , 3 - , 6 - , and 9 - day PHI s. Total combined residues of acequinocyl (corrected for decline during storage) and acequinocyl OH (calculated in parent equivalents) in/on cantaloupe ranged from <0.025 - <0.122 ppm, harvested 1 day

f ollowing foliar applications of the SC formulation for a total rate of 0.602 - 0.833 lb ai/A (Table 9) . The residue - decline trial (PHI = 0, 1, 3, 6, and 9 day) showed that residues declined from 0 - 3 day s, then remained <LLMV . Conclusions : The submitted cantaloupe field trial data are acceptable and reflect the use of the acequinocyl 1.25 lb/gal SC formulation as two broadcast foliar applications for a total rate of 0.600 - 0.609 lb ai/A (trial - OH*01 reflected three applications for a total of 0.833 lb ai/A) , with a 1 - day PHI. . Total combined residues of acequinocyl (corrected for decline during storage) and acequinocyl OH in/on cantaloupe ranged from <0.025 - <0.122 ppm, harvested 1 day following foliar applications of the SC formulation for a total rate of 0.602 - 0.833 lb ai/A. The residue - decline trial (PHI = 0, 1, 3, 6, and 9 day) showed that residues declined from 0 - 3 day s, then remained <LLMV. The residue data for cantaloupe were entered into the OECD MRLCalculator. Based on the results of these tria ls, the appropriate tolerance for residues of acequinocyl and acequinocyl - OH in/on cantaloupe is 0. 15 ppm. As cantaloupe is the representative commodity for melon subgroup 9A, the tolerance can be expanded to this subgroup . **A revised Section F is request**  **ed.** Cherry IR - 4 has submitted data from 12 cherry field trials on acequinocyl during the 2006 growing season. Six tart cherry

field trials were conducted in NAFTA Growing Zone 1 (NY; 1 trial), Zone 5 (MI, 4 trials), and Zone 9 (CO; 1 trial). Six sweet cherry field trials were conducted in NAFTA Growing Zone 5 (MI ; 2 trials), Zone 10 (CA, 2 trials), and Zone 11 (WA; 2 trials). At the trial sites, a 1.25 lb ai/gal SC formulation of acequinocyl was applied as 

### two foliar directed applications at a rate of ~0.303 lb ai/A each, for a total of 0.5836 - 0.6211 lb ai/A. The applications were made at 13 - to 15 - day RTIs and timed so that marketable pitted cherries could be collected at an approximately 7 - day PHI after the second application. Additional samples for decline determination were collected from the MI08 (tart) and CA29 (sweet) trials at 2 - or 3 - , 10 - , and 14 - day PHIs. Applications were made using ground equipment in spray

volumes of ~40 - 315 gal/A, without a spray adjuvant. Total combined residues of ac equinocyl and acequinocyl - OH (and per - trial averages) in/on tart cherries ranged from <0.160 - <0.481 ppm (<0.170 - <0.461 ppm), harvested 6 - 7 day s following foliar directed applications of the SC formulation for a total rate of 0.6042 - 0.6211 lb ai/A. Total c ombined residues of acequinocyl and acequinocyl - OH (and per - trial averages) in/on sweet cherries ranged from <0.060 - <0.216 ppm (<0.062 - <0.216 ppm), harvested 6 - 7 day s following foliar applications of the SC formulation for a total rate of 0.5836 - 0.6204 lb ai/A (Table 9) . InAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 18 of 30 general, residues were higher in tart cherries than in sweet cherries. Both the tart and sweet cherry residue - decline trials (PHI = 0, 2 or 3, 6 or 7, 10, and 14 day) showed that residues increased slightl y at the first interval, and then declined. Conclusions: The submitted cherry field trial data (6 trials on tart cherries, 6 trials on sweet cherries) are acceptable and reflect the use of the acequinocyl 1.25 lb ai/gal SC formulation as two foliar directed applications for a total rate of 0.5836 - 0.6211 lb ai/A, with a 6 - 7 - day PHI . Additional samples for decline determination were collected from two trials at 2 or 3, 6 or 7, 10, and 14 day s. Total combined residues of acequinocyl and acequinocyl - OH (and per - trial averages) in/on tart cherries ranged from <0 .160 - <0.481 ppm (<0.170 - <0.461 ppm), harvested 6 - 7 day s following foliar directed applications of the SC formulation for a total rate of 0.6042 - 0.6211 lb ai/A. Total combined residues of acequinocyl and acequinocyl - OH (and per - trial averages) in/on sweet cherries ranged from <0.060 - <0.216 ppm (<0.062 - <0.216 ppm), harvested 6 - 7 day s following foliar applications of the SC formulation for a total rate of 0.5836 - 0.6204 lb ai/A. In general, residues were higher in tart cherries than in sweet cherries. Both the tart and sweet cherry residue - decline trials (PHI = 0, 2 or 3, 6 or 7, 10 , and 14 day) showed that residues increased slightly at the first interval, and then declined. The residue data for tart and sweet cherries were separately entered into the OECD MRL Calculator. Based on the results of these trials, the appropriate tolerance s for residues of acequinocyl and acequinocyl - OH are 1.0 ppm in/on cherry , tart and 0.50 ppm in/on cherry, sweet . **A revised Section F is requested.** Cucumbers IR - 4 has submitted field trial and greenhouse data for acequinocyl in/on cucumbers. Nine supervised crop field trials in/on field cucumbers (pickling and slicing varieties) were conducted in the U.S. during the 2007 growing season encompassing NAFTA Growi ng Zones 2 (2 trials in GA, 1 trial in NC), 5 (2 trials in OH), 6 (1 trial in TX) and 10 (2 trials in CA). At each trial site, acequinocyl [formulated as Kanemite TM 15 SC] was applied to field cucumbers as 2 foliar broadcast or foliar directed applications at rates of 0.263 - 0.325 pounds ai /A, at RTI s of 19 - 23 day s, for a total rate of 0.567 - 0.636 pounds ai /A/season. The applications were made in spray volumes ranging from 32 - 77 gallons/A. No adjuvants were used in any of the spray

mixtures. Commercially mature field cucumbers were harvested at a PHI of 1 day . At the GA*15 trial site, field cucumbers were harvested at additional PHIs of 0, 4, 7, and 9 day s to assess residue - decline behavior. In addition, two greenhouse cucumber trials were conducted in the U.S. and Canada during the 2007 growing season encompassing NAFTA Growing Zones 2 (1 trial in NJ) and 5 (1 trial in ON). At each trial site, acequinocyl (formulated as Kanemite TM 15 SC) was applied to greenhouse cucumbers as two foliar - directed applicat ions at rates of 0.296 - 0.303 pounds ai /A, at RTI s of 19 - 20 day s, for a total rate of 0.597 - 0.604 pounds/A/season. The applications were made in spray

volumes r anging from 53 - 188 gallons/A . No adjuvants were used in an y of the spray mixtures. Commerciall y mature greenhouse cucumbers were harvested at a PHI of 1 day. At the ON01 trial site, greenhouse cucumbers were harvested at additional PHI s of 0, 3, 7, and 9 day s to assess residue - decline behavior.Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 19 of 30 Total residues of acequinocyl (corrected for storage dissipation) and acequinocyl - OH in/on field cucumbers treated at total rates of 0.567 - 0.625 pounds ai /A/season (636 - 701 g ai /ha/season) and harvested at a PHI of 1 day ranged from <0.02 - <0.069 ppm (Table 9) . Residue - decline data indicated that total residues of acequinocyl and acequinocyl - OH in/on field cucumbers declined with increasing PHIs. Total residues of acequinocyl (corrected for storage dissipation) and acequinocyl - OH in/on greenhouse cucumbers treated at total rates of 0.597 - 0.604 pounds ai /A/season (669 - 677 g ai /ha/season) and harvested at a PHI of 1 day ranged from <0.029 - <0.093 ppm (Table 9) . Residue - decline data indicated that total residues of acequinocyl and acequinocyl - OH in/on greenhouse cucumbers declined with increasing PHI s. Con clusions: The submitted crop field trials for acequinocyl in/on field cucumbers and greenhouse cucumbers are considered scientifically valid. Kanemite TM 15 SC was applied to field cucumbers as two foliar (broadcast or directed) applications at rates total rates of 0.567 - 0.625 lbs ai /A and mature field cucumbers were harvested at a PHI of 1 day . Total residues of acequinocyl (corrected for storage dissipation) and acequinocyl - OH in/on field cucumbers ranged from <0.02 - <0.069 ppm. Residue decline data indi cated that total residues of acequinocyl and acequinocyl - OH in/on field cucumbers declined with increasing PHIs. Kanemite TM 15 SC was also applied to greenhouse cucumbers as two foliar (broadcast or directed) applications at total rates of 0.597 - 0.604 lbs ai /A and mature greenhouse cucumbers were harvested at a PHI of 1 day . Total residues of acequinocyl (corrected for storage dissipation) and acequinocyl - OH in/on greenhouse cucumbers ranged from <0.029 - <0.093 ppm. Residue decline data indicated that tota l residues of acequinocyl and acequinocyl - OH in/on greenhouse cucumbers declined with increasing PHI s. The residue data for field and greenhouse cucumbers were entered into the OECD MRL Calculator (see ChemSAC minutes, 19 - OCT - 2011). Running the OECD MRL Calculator both with and without the greenhouse cucumber data resulted in the same tolerance level of 0.15 ppm. Based on the results of these trials, the appropriate tolerance for residues of acequinocyl and acequinocyl - OH is 0.15 ppm in/on cucumber . **860.1520 Processed Food and Feed** There are no processed food/feed items associated with the proposed uses. Therefore, data requirements for this guideline are not relevant to this tolerance petition. **860.1650 Submittal of Analytica l Reference Standards**  Analy tical reference standards for acequinocyl and acequinocyl - OH are currentl y available at the EPA National Pesticide Standards Repository ( electronic communication with Theresa Cole, 31 - OCT - 20 11) with expiration dates of June, 201 2. **860.1850/1900 Confined and Field Accumulation in Rotational Crops** The proposed uses under this petition includ e succulent soy beans, berries (subgroup 13 - 07G), succulent shelled beans, cowpea forage, cucumbers, and melons (subgr oup 9A) which are ty picall y rotated crops. An acceptable confined rotational crop study was previously

submitted ( Memo, S. Lev y, 25 - JUN - 2008; DP#s 351336 & 353313) in support of uses on strawberries .Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 20 of 30 The study results (47332501.der) showed the extent and nature of the residue uptake b y crops (lettuce, turnip and wheat) planted 30 - , 120 - and 365 - day s post - application in soil treated with 14 C - acequinocyl at 0.60 lb ai/A (1x maximum registered/proposed seasonal application rate to rotated crops ) . The availa ble data support a 30 - day PBIfor all non - labeled crops , which is reflected on the proposed label. **860.1550 Proposed Tolerances** Tolerances are currentl y established under 40 CFR §180.599(a) for the combined residues of acequinocyl and its metabolite, 2 - dodecyl - 3 - hy droxy- 1,4 - naphthoquinone, expressed as acequinocyl equivalents, in/on a number of plant and livestock commodities. Tolerances for plant commodities range from 0.02 ppm (tree nuts and pistachios) to 30 ppm (citrus oil), and tolerances for liv estock commodities are established at 0.02 ppm in the fat and liver of cattle, goat, horse, and sheep. Compliance with the tolerance levels specified below in Table 10 is to be determined b y measuring acequinocyl and its metabolite, 2 - dodecyl - 3 - hy droxy- 1, 4 - naphthoquinone, expressed as acequinocyl equivalents . There are currently

no established Codex, Canadian, or Mexican MRL s for acequinocyl residues in/ on cucumbers or caneberries. Data for these crops were jointly reviewed by

the U.S. and Canada. The d ata support tolerance levels of 0.15 ppm and 0.40 ppm, respectively . These levels are harmonized between the U.S. and Canada. C anad a has a MRLof 0.5 ppm for residues in/on strawberries. T he OECD Calculator shows that the data support a tolerance of 0 .40 ppm for residues in/on the low - gr owing berry subgroup 13 - 07G (which includes strawberries); however, in order to harmonize with Canada, HED recommends establishing a tolerance of 0.50 ppm for residues in this subgroup. Canada also has a MRL for residu es o f 0.02 ppm in/on meat by products of cattle, goat, horse, and sheep. HED is recommending for the same tolerance level as Canada ; therefore, harmoniz ation will not be an issue . **Table 10**  **. Tolerance Summary for**  **Acequinocyl .**  Commodity Proposed Tolerance (ppm) HED Recommended Tolerance (ppm) Comments ;

C orrect Commodity D efinition Soy bean, vegetable, succulent 0.25 0.25 Established edible - podded bean tolerance extended to soy bean, vegetable, succulent. Lo w - gr ow ing berry subgroup 13 - 07G 0.4 0. 5 0 Established straw berry tolerance extended for a subgroup tolerance. With establishment of this subgroup tolerance, the strawberry tolerance should be removed. Sm all fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit 1.6 1.6 Established grape tolerance extended for a subgroup tolerance . With establishment of this subgroup tolerance, the grape tolerance should be rem oved. Bean, succulent shelled 0.15 0.3 0Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 21 of 30 **Table 10**  **. Tolerance Summary for**  **Acequinocyl .**  Commodity Proposed Tolerance (ppm) HED Recommended Tolerance (ppm) Comments ;

C orrect Commodity D efinition Cow pea, forage 9.0 6.0 Cow pea, hay -- 18 Caneberry subgroup 13 - 07A 4.5 4.0 Melon subgroup 9A 0.06 0.15 Cucumber 0.15 0.15 Cherry 0.8 1.0 Cherry, tart

-- 0.5 0 Cherry, sweet Liver of cattle, goat, horse, and sheep 1

-- 0.02 Meat byproducts of cattle, goat, horse and sheep 1 A tolerance of 0.02 ppm is already established under 40 CFR §180.599 ; however, the commodity definition should be changed to meat byproducts to cover residues in liver and kidney . Conclusions : The petitioner should submit a revised Section F with the appropriate commodity definitions and/or HED - recommended to lerance levels listed in Table 15 . Furthermore, the Section F should state the following (as it appears in 40 CFR §180.599 ): “Tolerances are established for residues of acequinocyl, including its metabolites and degradates, in or on the commodities in th e table below. Compliance with the tolerance levels specified below is to be determined b y measuring only the sum of acequinocyl [ 2 - (acet yl oxy) - 3 - dodecyl - 1,4 - naphthalenedione] and its metabolite, 2 - dodecyl - 3 - hy droxy - 1,4 - naphthoquinone , calculated as the s toichiometric equivalent of acequinocyl, in or on the commodity .” **A revised Section F should be submitted**  **. References**  DP#: 297872 Subject: **Acequinocyl** . Report of the Metabolism Assessment Review Committee

(MARC). Meeting Date: 17 - DEC - 2003. From: S. L ev y/P. Terse Dated: 07 - JAN - 2004 MRID: None DP#: 298917 From: S. L ev y Dated: 18 - FEB - 2004 MRID : 45651603 DP#s: 284757, 290204, 286425 Subject: Acequinocyl. Registration for Use on Pome Fruits, Citrus, Almonds, Pistachios,

and Strawberry . Summ ary of Anal yt ical Chemistry

and Residue Data. From: S. L ev y Dated: 10 - MAR - 20 04 MRID(s): 46602001 - 46602003 DP#: 321753Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 22 of 30 Subject: Acequinocyl. Response to Submission of MRI D#s 46602001, 46602002 and

46602003 by

Morse Laboratories Addressing a Need for Co nfirmation of Acequinocyl in Fruits, Almonds and L ivestock Tissues. From: D. Soderberg and S. Levy Dated: 23 - MAY - 20 06 MRID(s): 46602001 - 46602003 DP# s : 330439, 337218, 337472 Subject: Acequinocyl. Petitions for Uses on Tree Nuts and Grapes. Summary of Analy tical Chemistry

and Residue Data. From: S. L ev y Dated: 05 - SEP - 20 07 MRID(s): 46776602, 47032402, & 46952301 DP#s: 351336 & 353313 Subject: Acequinocyl; Review of Petitioner’s Amendment Dated 28 - JUN - 2008 Submitted

in Response to the Health Effects Division (HED) Memoranda, DP# 284757 (dated 10 - MAR - 2004). From: S. L ev y Dated: 25 - JUN - 20 08 MRID: 47332501 DP#: 380302 Subject: **Acequinocyl** ; Response to the Health Effects Division (HED) Review of 25 - JUN 2008 (M emoranda, DP#s D351336 & D353313 ). From: S. L ev y Dated: 25 - AUG - 2010 MRID(s): 48016603 - 48016605 **Attachments:** International Residue Limit Status (IR LS) S heet . Appendix I - Tolerance Assessment Calculations .

RDI: G. Kramer ( 02 - NOV - 201 1 ); RAB1 Chemists ( 09 - NOV - 201 1 ) S. Levy:S - 10953:PY1:(703)305 - 0783:7509PAcequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 23 of 30 

### IRLS Sheet. Acequinocyl (PC Code 006329; 10/28/2011) Summary of U.S. and International Tolerances and Maximum Residue Limits Residue Definition : U.S. Canada Mexico 2 Codex 3 40 CFR 180.599 Plants/Livestock: sum of acequinocyl [2 - (acetyloxy) - 3 - dodecyl - 1,4 - naphthalenedione] and its metabolite, 2 - dodecyl - 3 - hydroxy - 1,4 - naphthoquinone, calculated as the stoichiometric equivalent of acequinocyl 2 - (acetyloxy) - 3 - dodecyl 1,4 - naphthalenedione, includi ng the metabolite 2 - dodecyl - 3 - hydroxy - 1,4 - naphthalenedione None

Commodity 1 Tolerance (ppm) /Maximum Residue Limit (mg/kg) U.S. Canada Mexico 2 Codex 3 Soy bean, vegetable, succulent 0.25 Lo w - gr ow ing berry subgroup 13 - 07G 0.40 0.5 straw berries Sm all fruit vine climbing subgroup 13 - 07F, except fuzzy kiwifruit 1.6 Bean, succulent shelled 0.30 Cow pea, forage 6.0 Cow pea, hay 18 Caneberry subgroup 13 - 07A 4.0 Melon subgroup 9A 0.15 Cucumber 0.15 Cherry, sweet 0. 50 Cherry, tart 1.0 Meat byproducts of cattle, goat, horse, and sheep 0.02 0.02 Meat byproducts of cattle, goat, horse, and sheep Completed: M. Negussie; 10/31/2011 1 Includes only commodities of interest for this action. Tolerance values should be the HED recommendations and not those proposed by the applicant. 2 Mexico adopts U.S. tolerances and/or Codex MRLs for its export purposes. 3 * = absent at the limit of qua ntitation; Po = postharvest treatment, such as treatment of stored grains. PoP = processed postharvest treated commodity, such as processing of treated stored wheat. (fat) = to be measured on the fat portion of the sample. MRLs indicated as proposed have not been finalized by the CCPR and the CAC.Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 24 of 30 **Appendix I . Tolerance Assessment Calculations.**  Succulent Shelled Beans The submitted U.S. residue data for succulent shelled beans (seeds without pods) and succulent bean plants with pods (7 data points ea ch ) were entered into the OECD MRL Calculator in order to determine appropriate tolerance levels. **Compound Crop**  **Region / Country GAP**  **Total number of data (n) Percentage of censored data**  **Number of non -**  **censored data Lowest residue**  **Highest residue**  **Median residue Mean**  **Standard deviation (SD) Correction factor for censoring**  **(CF) Proposed MRL estimate** 

**- Highest residue** 

**- Mean + 4 SD** 

**- CF x 3 Mean**  **Unrounded MRL Rounded MRL**  **Acequinocyl Bean, succulent**  **shelled (seeds**  **w/out pods )**

**USA 0.610**  **- 0.911**  7 0% 7 0.060 0.173 0.060 0.081 0.043 1.000

0.173 0.251 0.243 0.251 **0.3 Residues (mg/kg)**  0.060 0.060 0.173 0.095 0.060 0.060 0.060Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 25 of 30 **Compound Crop**  **Region / Country GAP**  **Total number of data (n) Percentage of censored data**  **Number of non -**  **censored data Lowest residue**  **Highest residue Median residue**  **Mean Standard deviation (SD)**  **Correction factor for censoring (CF)**  **Proposed MRL estimate**

**- Highest residue** 

**- Mean + 4 SD** 

**- CF x 3 Mean**  **Unrounded MRL Rounded MRL**  **Residues (mg/kg)**  0.527 1.690 2.590 2.460 0.952 0.345 2.510 **Acequinocyl Cowpea forage**  **(bean plants w/pods)**  **USA 0.610**  **- 0.911**  7 0% 7 0.345 2.590 1.690 1.582 0.975 1.000

2.590 5.481 4.746 5.481 **6**Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 26 of 30 Caneberry

Subgroup 13 - 07A The submitted U.S. residue data for raspberry (5 data points) and blackberry ( 2 data points) were entered into the OECD MRLCalculator in order to determine an appropriate crop group tolerance level. **Compound Crop**  **Region / Country GAP**  **Total number of data (n) Percentage of censored data**  **Number of non -**  **censored data Lowest residue**  **Highest residue Median residue**  **Mean Standard deviation (SD)**  **Correction factor for censoring (CF)**  **Proposed MRL estimate**

**- Highest residue** 

**- Mean + 4 SD** 

**- CF x 3 Mean**  **Unrounded MRL Residues (mg/kg)** 

0.280 1.910 1.474 0.325 0.801 **Acequinocyl Raspberries** 

**USA 0.584**  **- 0.612**  5 0% 5 0.280 1.910 0.801 0.958 0.717 1.000

1.910 3.827 2.874 3.827 **4**Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 27 of 30 Melon Subgroup 9A The submitted U.S. residue data for cantaloupe (8 data points) were entered into the OECD MRL Calculator in order to determine an appropriate crop group tolerance level. **Compound Crop**  **Region / Country GAP**  **Total number of data (n) Percentage of**  **censored data Number of non**  **- censored data**  **Lowest residue Highest residue**  **Median residue Mean**  **Standard deviation (SD) Correction factor for censoring**  **(CF) Proposed MRL estimate** 

**- Highest residue** 

**- Mean + 4 SD** 

**- CF x 3 Mean**  **Unrounded MRL Residues (mg/kg)** 

0.027 0.047 0.039 0.039 0.061 0.027 0.030 0.035 **Acequinocyl Melon subgroup 9A**  **(cantaloupe) USA**  **0.602 -**  **0.833** 8 0% 8 0.027 0.061 0.037 0.038 0.012 1.000

0.061 0.084 0.114 0.114 **0.15**Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 28 of 30 Cucumber The submitted U.S. residue data for field (8 data points) cucumbers were entered into the OECD MRLCalculator in order to determine an appropriate tolerance level. **Compound Crop**  **Region / Country GAP**  **Total number of data (n) Percentage of censored data**  **Number of non -**  **censored data Lowest residue**  **Highest residue Median residue**  **Mean Standard deviation (SD)**  **Correction factor for censoring (CF)**  **Proposed MRL estimate**

**- Highest**  **residue**

**- Mean + 4 SD** 

**- CF x 3 Mean**  **Unrounded MRL Residues (mg/kg)** 

0.020 0.031 0.060 0.058 0.020 0.061 0.056 0.031 0.069 0.030 **Acequinocyl Cucumbers**  **(field and greenhouse**  **)**

**USA 0.567**  **- 0.625** 

10 0% 10 0.020 0.06 9 0.044 0.04 4 0.01 9 1.000

0.06 9 0.11 9 0.1 31 0.1 31 **0.15**Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 29 of 30 Cherry The submitted U.S. residue data for sweet ( 6 data points) and tart ( 6 data points) cherries were entered into the OECD MRLCalculator in order to determine appropriate t olerance level s . **Compound Crop**  **Region / Country GAP**  **Total number of data (n) Percentage of censored data**  **Number of non -**  **censored data Lowest residue**  **Highest residue**  **Median residue Mean**  **Standard deviation (SD) Correction factor for censoring**  **(CF) Proposed MRL estimate** 

**- Highest residue** 

**- Mean + 4 SD** 

**- CF x 3 Mean**  **Unrounded MRL Residues (mg/kg)** 

0.118 0.151 0.192 0.216 0.105 0.062 **Acequinocyl Cherry, sweet** 

**USA 0.5836**  **- 0.6204**  6 0% 6 0.062 0.216 0.135 0.141 0.057 1.000

0.216 0.370 0.422 0.422 **0.5**Acequinocyl Summary of Analytical Chemistry and Residue Data DP#: 389989

Page 30 of 30 **Compound Crop**  **Region / Country GAP**  **Total number of data (n) Percentage of censored data**  **Number of non -**  **censored data Lowest residue**  **Highest residue Median residue**  **Mean Standard deviation (SD)**  **Correction factor for censoring (CF)**  **Proposed MRL estimate**

**- Highest residue** 

**- Mean + 4 SD** 

**- CF**  **x 3 Mean Unrounded MRL**  **Residues (mg/kg)**

0.315 0.460 0.461 0.170 0.270 0.240 **Acequinocyl Cherry, tart** 

**USA 0.6024**  **- 0.6211**  6 0% 6 0.170 0.461 0.293 0.319 0.119 1.000

0.461 0.796 0.958 0.958 **1**