### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 1 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2172 Alexander Springs Creek 2917 Boyd Lake Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH threshold (> 0.3 mg/kg) 3a 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Bluegill. 12 Bluegill samples; Hg mean concentration was 0.60 mg/Kg in 2002. *

09-2173 Deep Creek 2893F St Johns River Above Lake Jesup Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 3c 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 34 Largemouth Bass samples; Hg mean concentration was 0.43 mg/Kg in 2004. *

09-2174 Deep Creek 2893H Mullet Lake Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 34 Largemouth Bass samples; Hg mean concentration was 0.43 mg/Kg in 2004. *

09-2175 Deep Creek 2893J Mud Lake Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2176 Deep Creek 2925 Deep Creek / Lake Ashby Canal Stream 3F Dissolved Oxygen BOD median TN = 1.109 mg/L

(n=141), median TP =

0.12 mg/L (n=145), median BOD=2.4 mg/L (n=21) ≥ 5.0 mg/L 4c 5 5 Impaired Medium 45/134 DO met the verification threshold and BOD was identified as the causative pollutant. This waterbody is likely a blackwater stream.

09-2177 Deep Creek 2925 Deep Creek / Lake Ashby Canal Stream 3F Nutrients (Historic Chlorophyll-a) median TN = 1.109 mg/L

(n=141), median TP =

0.12 mg/L (n=145), median BOD=2.4 mg/L (n=21) Historical Threshold + 50%:

4.9005 μg/L N/A 5 5 Impaired Medium Chl-a annual means 18.72, 1.75, 1.47, 1.63, 1.2, 10.22, 14.42 μg/L (2001-2007) For the historical listing (1995-1999), annual mean Chl-a values exceeded the minimum historical annual average value of 3.267 μg/L by more than 50% in 2007, and the exceedance value was 14.422 μg/L. TN is the limiting nutrient based on

a median TN/TP ratio of 8.03 (n=141). This waterbody is likely a blackwater stream.

09-2178 Deep Creek 2925A Lake Ashby Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 20 Largemouth Bass samples; Hg mean concentration was 0.62 mg/Kg in 2002. *

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 2 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2179 Deep Creek 2925A Lake Ashby Lake 3F Nutrients (TSI Trend) slope≤ 5 TSI units N/A 5 5 Impaired Medium Annual mean TSI: 38.1, 38.6, 39.7, 42.3, 44.9, 43.1, and 44.5 (2001-2007). Color values are 167, 218, 231, 236, 279, 172, 80.2 (2001- 2007). vp = TSI annual means increased by more than 5 units over the verified period and increased as indicated by a positive slope in the means plotted versus timewhen using a Mann's one-sided uppertail test for trend.

09-2180 Deep Creek 2931 Lake Winnemisset t Lake 3F Nutrients (Historic TSI) median TN = 0.3685 mg/L

(n=48), median TP =

0.01 mg/L

(n=49), median BOD=No

data. Historical Threshold + 50%: TSI 28.56 N/A 5 5 Impaired Medium Annual mean TSI: 23.0, 24.5, 23.3, 28.9, 34.6, and 30.6 (2001-

2006). Color values are 10, 10, 11.3, 12.5,

15, and 10 (2001-2006). For the historical listing (1990-1994), annual mean TSI exceeded the minimum historical annual average value of 19.04

by more than 50% in 2005 and 2006, and the exceedance values were 34.56

and 30.59, respectively. TP is the limiting nutrient based on a median TN/TP ratio of 37.8 (n=48).

09-2181 Deep Creek 2952 Cow Creek Stream 3F Dissolved Oxygen Total Nitrogen, BOD median TN = 1.614 mg/L

(n=76), median TP = 0.064 mg/L

(n=78), median BOD=2.1 mg/L (n=9) ≥ 5.0 mg/L N/A 5 5 Impaired Medium 59/73 DO met the verification threshold and total nitrogen and BOD were identified as the causative pollutants. This waterbody is likely a blackwater stream.

09-2182 Deep Creek 2964 St Johns River Above Lake Harney

(Underhill Slough) Stream 3F Dissolved Oxygen Total Nitrogen median TN = 1.789 mg/L

(n=59), median TP = 0.088 mg/L

(n=62), median BOD=1.9 mg/L (n=16) ≥ 5.0 mg/L 4c 5 5 Impaired Medium 15/52 DO met the verification threshold and total nitrogen was identified as the causative pollutant.

09-2183 Deep Creek 2964 St Johns River Above Lake Harney

(Underhill Slough) Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 34 Largemouth Bass samples; Hg mean concentration was 0.43 mg/Kg in 2004. *

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 3 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2184 Deep Creek 2964A1 Lake Harney Outlet Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 34 Largemouth Bass samples; Hg mean concentration was 0.43 mg/Kg in 2004. *

09-2185 Econlockhatc hee River 2991 Econlockhatc hee River Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 3c 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.72 mg/Kg in 2007. *

09-2186 Econlockhatc hee River 2991A Econlockhatc hee River Stream 3F Mercury (In fish tissue) Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 3c 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.72 mg/Kg in 2007. *

09-2187 Econlockhatc hee River 2991B Buck Lake Lake 3F Nutrients (TSI) median TN = 0.905 mg/L

(n=51), median TP = 0.0225 mg/L

(n=56), median BOD=2 mg/L (n=56) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual mean TSIs were 46.4

(2001), 43.6 (2003), 40.6 (2004), 53.0 (2006), and 47.1 (2007). Color value is 38.6 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 47.1. TP is the limiting nutrient based on a median TN/TP ratio of 35.48 (n=51).

09-2188 Econlockhatc hee River 3021 Unnamed Branch Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL 2 5 5 Impaired Low 7/24 Fecal coliforms met verification thresholds.

09-2189 Econlockhatc hee River 3023C Lake Susannah Lake 3F Nutrients (TSI Trend) slope≤ 5 TSI units N/A 5 5 Impaired Medium Annual mean TSI: 30.8 (2001), 31.8 (2005), 35.6 (2006), and 40.5 (2007). There is no color data available. vp = TSI annual means increased by more than 5 units over the verified period and increased as indicated by a positive slope in the means plotted versus timewhen using a Mann's one-sided uppertail test for trend.

09-2190 Econlockhatc hee River 3023D Lake Gear Lake 3F Nutrients (TSI) median TN = 0.67 mg/L

(n=30), median TP = 0.027 mg/L

(n=31), median BOD=2.3 mg/L (n=38) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 39.5 (2006) and 44.5 (2007). Color value is 31.3 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 44.5. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 26.2 (n=27).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 4 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2191 Econlockhatc hee River 3023E Lake Barton Lake 3F Nutrients (TSI) median TN = 0.594 mg/L

(n=33), median TP = 0.019 mg/L

(n=32), median BOD=2.4 mg/L (n=15) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual means were 37.3 (2005), 35.2 (2006), and 49.8 (2007). Color values are 20.9 (2005) and 13.9 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 49.8. TP is the limiting nutrient based on a median TN/TP ratio of 41.6 (n=32).

09-2192 Econlockhatc hee River 3036 Lake Frederica Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 20 Largemouth Bass samples; Hg mean concentration was 0.31 mg/Kg in 2002. *

09-2193 Econlockhatc hee River 3037 Unnamed Drain Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL N/A 5 5 Impaired Low 15/30 Fecal coliforms met verification thresholds.

09-2194 Lake George 2213O St Johns River Above Ocklawaha River Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 2 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 312 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2196 Lake George 2213O St Johns River Above Ocklawaha River Stream 3F Un-ionized Ammonia ≤ 0.02 mg/L as NH3 N/A 5 5 Impaired Medium 11/58 Un-ionized ammonia met verification thresholds. Although pH exceeded Department's thresholds, the pH was determined to fall within the natural range for this waterbody.

09-2197 Lake George 2893A Lake George Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 2 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2198 Lake George 2893A1 St Johns River Below Lake George Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2199 Lake George 2893A2 St Johns River Above Lake George Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 5 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2200 Lake George 2893A3 Lake George Leftover Stream 3F Dissolved Oxygen Total Nitrogen, BOD median TN = 1.695 mg/L

(n=32), median TP = 0.073 mg/L

(n=35), median BOD=2.25 mg/L (n=34) ≥ 5.0 mg/L 3b 5 5 Impaired Medium 14/36 DO met the verification threshold and total nitrogen and BOD were identified as the causative pollutants.

09-2201 Lake George 2893A3 Lake George Leftover Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2202 Lake Jesup 2982 Phelps Creek Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL N/A 5 5 Impaired Low 14/35 Fecal coliforms met verification thresholds.

09-2203 Lake Jesup 2984 Six Mile Creek (Lake Nan) Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL 2 5 5 Impaired Low 9/46 Fecal coliforms met verification thresholds.

09-2204 Lake Jesup 2985 Chub Creek Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL N/A 5 5 Impaired Low 10/13 Fecal coliforms met verification thresholds.

09-2205 Lake Jesup 2986 Soldier Creek Reach Stream 3F Coliforms Fecal Coliform ≤ 400 Counts / 100 mL 3c 5 5 Impaired Low 12/37 Fecal coliforms met verification thresholds.

09-2206 Lake Jesup 2986B Lake Myrtle Lake 3F Dissolved Oxygen BOD median TN = 0.988 (n=40), median TP = 0.024 mg/L

(n=47), median BOD=2.9 mg/L (n=45) ≥ 5.0 mg/L 2 5 5 Impaired Medium 11/40 DO met the verification threshold and BOD was identified as the causative pollutant.

09-2207 Lake Jesup 2990 Salt Creek Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL N/A 5 5 Impaired Low 7/30 Fecal coliforms met verification thresholds.

09-2208 Lake Jesup 2994A Gee Creek Stream 3F Coliforms Fecal Coliform ≤ 400 Counts / 100 mL 3c 5 5 Impaired Low 15/33 Fecal coliforms met verification thresholds.

09-2209 Lake Jesup 2994C Fairy Lake Lake 3F Nutrients (TSI) median TN = 0.655 mg/L

(n=39), median TP =

0.62 mg/L

(n=48), median BOD=2.25 mg/L (n=48) Annual average TSI >40 N/A 5 5 Impaired Medium Annual mean TSIs were 50.1 (2002) and 43.5

(2004), and 48.2 (2007). Color value is 16.3 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 48.2. TP is the limiting nutrient based on a median TN/TP ratio of 30.12 (n=39).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 6 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2210 Lake Jesup 2994E Red Bug Lake Lake 3F Nutrients (TSI) median TN = 0.765 mg/L

(n=51), median TP = 0.002 mg/L

(n=59), median BOD=1.9 mg/L (n=62) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 39.3 (2001), 36.7 (2002), 30.9 (2003), and 30.2 (2004),

and 40.2 (2007). Color value is 36.7 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 40.2. TP is the limiting nutrient based on a median TN/TP ratio of 40.7 (n=51).

09-2211 Lake Jesup 2994X Little Lake Howell Lake 3F Nutrients (TSI) median TN = 0.796 mg/L

(n=45), median TP =

0.02 mg/L

(n=55), median BOD=2 mg/L (n=51) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 46.1 (2001), 39.0 (2002), 51.1 (2004), 38.4 (2005), and 43.6 (2007). Color values are 61.7 (2005) and 27.5 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 43.6. TP is the limiting nutrient based on a median TN/TP ratio of 38.2 (n=44).

09-2212 Lake Jesup 2994Y1 Lake Tony Lake 3F Nutrients (TSI) median TN = 0.9915 mg/L

(n=34), median TP =

0.02 mg/L

(n=47), median BOD=2 mg/L (n=49) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 55.1 (2001), 49.4 (2003), 50.7 (2004), and 49.1 (2007). Color value is

25 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 49.1. TP is the limiting nutrient based on a median TN/TP ratio of 49.6 (n=34).

09-2213 Lake Jesup 2997 Howell Creek Below Lake

Howell Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL N/A 5 5 Impaired Low 18/60 Fecal coliforms met verification thresholds.

09-2214 Lake Jesup 29971 Leftover Lake Ivanhoe Lake 3F Nutrients (TSI) median TN = 0.64 mg/L

(n=39), median TP = 0.036 mg/L

(n=37), median BOD=2.6 mg/L (n=42) Annual average TSI >40 N/A 5 5 Impaired Medium Annual means were 52.4 (2001), 50.0 (2002), 48.7 (2006), and 52.4 (2007). Color values are 15 (2002)

and 17.9 (2007). Annual mean TSIs exceeded 40 in 2002 and 2007, and the values were 50 and42.4, respectively. TN and TP are colimiting nutrients based on a median TN/TP ratio of 19.5 (n=37).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 7 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2215 Lake Jesup 29975 Lake Sybella Lake 3F Nutrients (TSI) median TN = 0.59 mg/L

(n=30), median TP =

0.02 mg/L

(n=29), median BOD=2.05 mg/L (n=20) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual mean TSI was 47.0 (2007). Color value is 10.3 (2007). Annual mean TSI exceeded 40 in 2007,and the value was 47. TN and TP are colimiting nutrients based on a median TN/TP ratio of 28.8 (n=29).

09-2216 Lake Jesup 29977 Lake in the Woods Lake 3F Nutrients (TSI) median TN = 0.79 mg/L

(n=36), median TP = 0.033 mg/L

(n=56), median BOD=3 mg/L (n=56) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 54.3 (2001), 55.9 (2003), 59.2 (2004), and 51.3 (2007). Color value is 23.8 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 51.3. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 26.6 (n=37).

09-2217 Lake Jesup 2997B1 Lake Ann Lake 3F Nutrients (TSI) median TN = 0.84 mg/L

(n=46), median TP =

0.02 mg/L

(n=53), median BOD=3.3 mg/L (n=50) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 32.0 (2001), 48.6 (2003), 48.6 (2004), 48.7 (2005), and 54.8 (2007). Color values are 30.3 (2005) and 21.3 (2007). Annual mean TSIs exceeded 40 in 2005 and 2007, and the values were 48.7 and

54.8, respectively. TP is the limiting nutrient based on a median TN/TP ratio of 42 (n=44).

09-2218 Lake Jesup 2997I Lake Sue Lake 3F Nutrients (TSI) median TN = 0.55 mg/L

(n=21), median TP =

0.02 mg/L

(n=21), median BOD=2 mg/L (n=14) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual means were 51.8 (2002), 52.6 (2003), 46.9 (2005), 47.9 (2006), and 45.4 (2007). Color values are 15 (2002)

and 16.3 (2007). Annual mean TSIs exceeded 40 in 2002 and 2007, and the values were 51.8 and

45.7, respectively. TP is the limiting nutrient based on a median TN/TP ratio of 31.4 (n=21).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 8 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2219 Lake Jesup 2997J Lake Rowena Lake 3F Nutrients (TSI) median TN = 0.75 mg/L

(n=46), median TP = 0.026 mg/L

(n=43), median BOD=2.6 mg/L (n=41) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual mean TSIs were 52.0

(2001), 51.6 (2002), 45.7 (2005), 53.9 (2006), and 50.0 (2007). Color values are 10 (2001), 15 (2002), 20

(2005), 6.8

(2007). Annual mean TSIs exceeded 40 in 2001, 2002, 2005 and 2007, and the values were 52, 51.6, 45.7, and 50, respectively . TP is the limiting nutrient based on a median TN/TP ratio of 30.4 (n=43).

09-2220 Lake Jesup 2997K Lake Estelle Lake 3F Nutrients (TSI) median TN = 0.87 mg/L

(n=61), median TP = 0.032 mg/L

(n=52), median BOD=2.9 mg/L (n=69) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual means were 55.6 (2001), 56.1 (2002), 53.1 (2003), 47.1 (2005), 54.0 (2006), and 52.6 (2007). Color values are 17.5 (2002) and 20 (2007). Annual mean TSIs exceeded 40 in 2002 and 2007, and the values were 56.1 and

52.6, respectively. TP is the limiting nutrient based on a median TN/TP ratio of 30.4 (n=52).

09-2221 Lake Jesup 2997M Lake Formosa Lake 3F Nutrients (TSI) median TN = 0.78 mg/L

(n=38), median TP = 0.024 mg/L

(n=32), median BOD=2.7 mg/L (n=38) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual means were 46.6 (2001), 47.3 (2002), 40.5 (2005), 48.3 (2006), and 48.3 (2007). Color values are 10 (2001), 15 (2002), and

17.5 (2005). Annual mean TSIs exceeded 40 in 2001, 2002 and 2005, and the values were 46.6, 47.3, and 40.5, respectively. TP is

the limiting nutrient based on a median TN/TP ratio of 31.2 (n=32).

09-2222 Lake Jesup 2997O Park Lake Lake 3F Nutrients (TSI) median TN = 0.72 mg/L

(n=26), median TP = 0.058 mg/L

(n=25), median BOD=2.6 mg/L (n=29) TSI < 60; Color > 40 2 5 5 Impaired Medium Annual means were 52.0 (2001), 60.8 (2003), 53.0 (2006), and 54.3 (2007). Available color

data is 20

(2002). Annual mean TSI exceeded 60 in 2003, and the value was 60.8. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 12.9 (n=25).

09-2223 Lake Jesup 2997Q Lake Dot Lake 3F Fecal Coliform ≤ 400 Counts / 100 mL 3c 5 5 Impaired Low 9/23 Fecal coliforms met verification thresholds.

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 9 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2224 Lake Jesup 2997Q Lake Dot Lake 3F Nutrients (TSI) median TN = 0.73 mg/L

(n=59), median TP = 0.058 mg/L

(n=41), median BOD=2.8 mg/L (n=56) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 50.7 (2001), 45.5 (2003), 57.4 (2005), 55.5 (2006), and 51.9 (2007). Color value is 21.2 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 51.9. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 13.5 (n=49).

09-2225 Lake Jesup 2997U Lake Park Lake 3F Nutrients (TSI) median TN = 0.819 mg/L

(n=25), median TP =

0.02 mg/L

(n=25), median BOD=2.9 mg/L (n=25) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual means were 44.7 (2001) and 52.4 (2007). Color values are 12.5 (2001) and 16.6 (2007). Annual mean TSIs exceeded 40 in 2001 and 2007, and the values were 44.7 and

52.4, respectively. TP is the limiting nutrient based on a median TN/TP ratio of 39.8 (n=25).

09-2226 Lake Jesup 2997X Lake Killarney Lake 3F Nutrients (TSI) median TN = 0.6915 mg/L

(n=46), median TP = 0.022 mg/L

(n=45), median BOD=3.4 mg/L (n=31) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual means were 49.6 (2006) and 56.7 (2007). Color value is 13.1 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 56.7. TP is the limiting nutrient based on a median TN/TP ratio of 34 (n=45).

09-2227 Lake Jesup 2999A Lake Hayes Lake 3F Nutrients (TSI) median TN = 0.6657 mg/L

(n=40), median TP =

0.02 mg/L

(n=51), median BOD=2 mg/L (n=49) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 40.7 (2001), 48.7 (2003), 52.0 (2004), and 46.4 (2007). Color value is 22.3 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 46.4. TP is the limiting nutrient based on a median TN/TP ratio of 32.3 (n=40).

09-2228 Lake Jesup 3009C Lake Burkett Lake 3F Nutrients (TSI) median TN = 0.94 mg/L

(n=23), median TP = 0.0215 mg/L

(n=20), median BOD=2.3 mg/L (n=13) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual means were 45.4 (2001) and 50.9 (2007). Color

value is 30

(2001). Annual mean TSI exceeded 40 in 2001, and the value was 45.4 TP is the limiting nutrient based on a median TN/TP ratio of 44.3 (n=19).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 10 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2229 Lake Jesup 3009E Lake Georgia Lake 3F Nutrients (TSI) median TN = 0.775 mg/L

(n=34), median TP = 0.018 mg/L

(n=32), median BOD=0.85 mg/L (n=22) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 32.0 (2006) and 41.0 (2007). Color value is 16.9 (2007) Annual mean TSI exceeded 40 in 2007, and the value was 41. TP is the limiting nutrient based on a median TN/TP ratio of 48.5 (n=32).

09-2230 Lake Kerr 2894 Lake Delancey Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.90 mg/Kg in 2003. *

09-2231 Lake Kerr 2899B Lake Kerr Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 3c 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 14 Largemouth Bass samples; Hg mean concentration was 0.52 mg/Kg in 2002 and 2005. *

09-2232 Lake Kerr 2899B Lake Kerr Lake 3F Nutrients (TSI Trend) slope≤ 5 TSI units N/A 5 5 Impaired Medium Annual mean TSI: 21.7, 23.1, 25.4, 25.4, 28.0, and 28.1 (2001-

2006). Color values are 10, 11.3, 12.5, 15, 19.6, and 13.8 (2001-2006). vp = TSI annual means increased by more than 5 units over the verified period and increased as indicated by a positive slope in the means plotted versus timewhen using a Mann's one-sided uppertail test for trend.

09-2233 Lake Monroe 2893C St Johns River Above

Wekiva

River Stream 3F Biochemical Oxygen Demand Dissolved Oxygen Total Nitrogen, BOD median TN = 1.602 mg/L

(n=101), median TP = 0.094 mg/L

(n=119), median BOD=2.8 mg/L (n=12) ≥ 5.0 mg/L 2 5 5 Impaired Medium 26/119 DO met the verification threshold and total nitrogen and BOD were identified as

the causative pollutants. Delisted from the 1998 303(d) List in Cycle 1. Assessed as verified impaired in Cycle 2.

09-2234 Lake Monroe 2893C St Johns River Above

Wekiva

River Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 2 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 11 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2235 Lake Monroe 2893D Lake Monroe Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 2 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2236 Lake Monroe 2893E St Johns River Above Lake Monroe Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 3c 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 34 Largemouth Bass samples; Hg mean concentration was 0.43 mg/Kg in 2004. *

09-2237 Lake Monroe 2953A Broken Arrow Lake Lake 3F Nutrients (Historic TSI) median TN = 0.404 mg/L

(n=17), median TP =

0.02 mg/L

(n=20), median BOD=0.7 mg/L (n=23) Historical Threshold + 50%: TSI 25.88 N/A 5 5 Impaired Medium Annual mean TSI: 32.3 (2007). Color value is 12.1 (2007). For the historical listing (1990-1994), annual mean TSI exceeded the minimum historical annual average value of 17.25

by more than 50% in 2007, and the exceedance value was 32.27. TN and TP are co-limiting nutrients based on a

median TN/TP ratio of 19.2 (n=17).

09-2238 Lake Monroe 2954 Konomac Lake Reservoir Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2007. *

09-2239 Lake Monroe 2973 Deforest Lake Outlet Stream 3F Fecal Coliform ≤ 400 Counts / 100 mL 2 5 5 Impaired Low 6/20 Fecal coliforms met verification thresholds.

09-2240 Lake Monroe 2973 Deforest Lake Outlet Stream 3F Nutrients (Chlorophyll-a) median TN = 1.2732 mg/L

(n=20), median TP = 0.115 mg/L

(n=22), median BOD=2 mg/L (n=20) ≤ 20 μg/L N/A 5 5 Impaired Medium Chl-a annual

means were

33.5 μg/L (2003), and 5.375 μg/L

(2005). Annual mean exceeded 20 μg/L in 2003, and the value was 33.5 μg/L. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 13.12 (n=20). Additionally, nutrients were identified as

the causative pollutant for dissolved oxygen impairment in Cycles 1 and 2.

09-2241 Lake Monroe 2973 Deforest Lake Outlet Stream 3F Turbidity 35.8 NTU (20th Percentile > 29

NTU Above Background) 2 5 5 Impaired Medium 6/21 Turbidity met verification threshold for exceedances of 35.8 NTU; 29 NTU +

background (20th percentile, or 6.8 NTU).

09-2242 Lake Woodruff 28933 Blue Spring (Volusia County) Spring 3F Nutrients Nutrients (Algal mats) Balanced natural population of flora. N/A 5 5 Impaired Low 45/113 Nutrient impairment based on evidence of excessive algal smothering of the habitat. 45 samples > 0.6 mg/L for NO2 and NO3. A reference springs report is available.

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 12 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2243 Lake Woodruff 28933A Blue Spring Run (Volusia

County) Stream 3F Nutrients (Algal mats) Balanced natural population of flora. N/A 5 5 Impaired Low 45/113 Nutrient impairment based on evidence of excessive algal smothering of the habitat. 45 samples > 0.6 mg/L for NO2 and NO3. A reference springs report is available.

09-2244 Lake Woodruff 2893B St Johns River Above Lake Woodruff Stream 3F Dissolved Oxygen BOD median TN = 1.284 mg/L

(n=271), median TP =

0.08 mg/L (n=295), median BOD=2.6 mg/L (n=23) ≥ 5.0 mg/L 4c 5 5 Impaired Medium 141/304 DO met the verification threshold and BOD was identified as the causative pollutant.

09-2245 Lake Woodruff 2893B St Johns River Above Lake Woodruff Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 2 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2246 Lake Woodruff 2893Z St Johns River Below Lake Dexter

(St Johns River Above Lake George) Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 2 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.47 mg/Kg in 2005. *

09-2247 Lake Woodruff 2912A Lake Emporia Lake 3F Nutrients (Historic TSI) median TN = 0.844 mg/L

(n=27), median TP =

0.02 mg/L

(n=27), median BOD=2 mg/L (n=27) Historical Threshold + 50%: TSI 46.17 N/A 5 5 Impaired Medium Annual mean TSI was 47.97 (2007). Color value is 19.4 (2007). For the historical listing (1989-1993), annual mean TSI exceeded the minimum historical annual average value of 30.78

by more than 50% in 2007, and the exceedance value was 47.97. TP is the

limiting nutrient based on a median TN/TP ratio of 39.8 (n=27).

09-2248 Lake Woodruff 2921 Lake Woodruff Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.40 mg/Kg in 2004. *

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 13 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2249 Lake Woodruff 2921C Lake Dexter Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.40 mg/Kg in 2004. *

09-2250 Lake Woodruff 2921D Lake Woodruff

Outlet Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.430mg/Kg in 2004. *

09-2251 Wekiva River 2929B Lake Norris Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 16 Largemouth Bass samples; Hg mean concentration was 0.95 mg/Kg in 2000. *

09-2252 Wekiva River 2929C Lake Dorr Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) 3c 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 12 Largemouth Bass samples; Hg mean concentration was 0.36 mg/Kg in 2003. *

09-2253 Wekiva River 2934 Tracy Canal Stream 3F Dissolved Oxygen Total Nitrogen median TN = 2.845 mg/L

(n=74), median TP = 0.063 mg/L

(n=74), median BOD=0.7 mg/L (n=3) ≥ 5.0 mg/L N/A 5 5 Impaired Medium 70/71 DO met the verification threshold and total nitrogen was identified as the causative pollutant.

09-2254 Wekiva River 2934 Tracy Canal Stream 3F Turbidity 39.86 NTU (20th Percentile > 29

NTU Above Background) N/A 5 5 Impaired Medium 30/72 Turbidity met verification threshold for exceedances of 39.86 NTU; 29 NTU + background (20th percentile, or 10.86 NTU).

09-2255 Wekiva River 2956 Wekiva River Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 18 Largemouth Bass samples; Hg mean concentration was 0.59 mg/Kg in 2003. *

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 14 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2256 Wekiva River 2956A Wekiva River Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 18 Largemouth Bass samples; Hg mean concentration was 0.59 mg/Kg in 2003. *

09-2257 Wekiva River 2956A1 Linden Lake Lake 3F Dissolved Oxygen Total Nitrogen median TN = 1.861 mg/L

(n=19), median TP = 0.018 mg/L

(n=22), median BOD=2.45 mg/L (n=22) ≥ 5.0 mg/L N/A 5 5 Impaired Medium 8/21 DO met the verification threshold and total nitrogen was identified as the causative pollutant.

09-2258 Wekiva River 2956B Lower Wekiva

River Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 18 Largemouth Bass samples; Hg mean concentration was 0.59 mg/Kg in 2003. *

09-2259 Wekiva River 2956C Wekiwa Spring Stream 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 18 Largemouth Bass samples; Hg mean concentration was 0.59 mg/Kg in 2003. *

09-2260 Wekiva River 2956E Sand Lake Lake 3F Nutrients (TSI) median TN = 0.986 mg/L

(n=43), median TP =

0.02 mg/L

(n=49), median BOD=2 mg/L (n=48) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 44.4 (2001), 48.4 (2002), 53.0 (2003), and 45.5 (2007). Color value is

30 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 45.5. TP is the limiting nutrient based on a median TN/TP ratio of 50.8 (n=43).

09-2261 Wekiva River 2961 Lake Sylvan Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 20 Largemouth Bass samples; Hg mean concentration was 0.79 mg/Kg in 2001. *

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 15 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2262 Wekiva River 2998 Cranes Roost Outlet Stream 3F Nutrients (Chlorophyll-a) median TN = 1.2155 mg/L

(n=18), median TP = 0.355 mg/L

(n=18), median BOD=2.85 mg/L (n=20) ≤ 20 μg/L N/A 5 5 Impaired Medium Chl-a annual

means were 16.96 μg/L (2001), and 26.76 μg/L (2002), and 23.06 μg/L

(2007). Annual mean exceeded 20 μg/L in 2007, and the value was 23.06 μg/L. TN is the limiting nutrient based on a median TN/TP ratio of 3.21 (n=18).

09-2263 Wekiva River 3000 Lake Pearl Lake 3F Nutrients (TSI) median TN = 1.06 mg/L

(n=39), median TP = 0.033 mg/L

(n=37), median BOD=3.05 mg/L (n=24) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 48.9 (2006) and 56.9 (2007). Color value is 16.5 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 56.9. TP is the limiting nutrient based on a median TN/TP ratio of 31.3 (n=37).

09-2264 Wekiva River 3000A Lake Harriet Lake 3F Fecal Coliform ≤ 400 Counts / 100 mL N/A 5 5 Impaired Low 9/32 Fecal coliforms met verification thresholds.

09-2265 Wekiva River 3002J Lake Hiawassee Lake 3F Nutrients (TSI) median TN = 0.7 mg/L (n=59), median TP = 0.017 mg/L

(n=54), median BOD=1.6 mg/L (n=56) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual mean TSIs were 45.5

(2001), 50.3 (2002), 42.4 (2005)), 34.5 (2006), and 42.4 (2007). Color values were 20 (2002) and 15 (2007). Annual mean TSI exceeded 40 in 2002 and 2007, and the values were 50.3 and

42.4, respectively. TP is the limiting nutrient based on a median TN/TP ratio of 40.9 (n=53).

09-2266 Wekiva River 3004A Bear Lake Lake 3F Mercury (Based on fish consumption

advisory) Exceeds DoH Threshold (> 0.3 mg/kg) N/A 5 5 Impaired High N/A Confirmed recent data for freshwater fish advisory for Largemouth Bass. 14 Largemouth Bass samples; Hg mean concentration was 0.65 mg/Kg in 2002. *

09-2267 Wekiva River 3004A Bear Lake Lake 3F Nutrients (TSI) median TN = 0.8531 mg/L

(n=42), median TP = 0.019 mg/L

(n=50), median BOD=2 mg/L (n=49) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 41.2 (2001), 38.9 (2002), 33.1 (2003), 37.5 (2004), and 40.1 (2007). Color value is 10.3 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 40.1. TP is the limiting nutrient based on a median TN/TP ratio of 35.7 (n=42).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 16 of18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2268 Wekiva River 3004B Lake Fairview Lake 3F Nutrients (TSI) median TN = 0.635 mg/L

(n=36), median TP = 0.025 mg/L

(n=34), median BOD=2.64 mg/L (n=21) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual mean TSI was 51.7 (2007). Color value is 15.5 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 51.7. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 23.6 (n=33).

09-2269 Wekiva River 3004E Lake Daniel Lake 3F Nutrients (TSI) median TN = 0.745 mg/L

(n=22), median TP = 0.027 mg/L

(n=22), median BOD=2.8 mg/L (n=28) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual mean TSIs were 51.7

(2001), 53.4 (2002), and 43.4 (2006). Color value is

20 (2002). Annual mean TSI exceeded 40 in 2002, and the value was 53.4. TP is the limiting nutrient based on a median TN/TP ratio of 31.8 (n=21).

09-2270 Wekiva River 3004F Lake Sarah Lake 3F Nutrients (TSI) median TN = 0.68 mg/L

(n=37), median TP =

0.02 mg/L

(n=33), median BOD=2.35 mg/L (n=26) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual mean TSIs were 53.9

(2002), 44.2 (2005), 40.2 (2006), and 38.2 (2007). Color values are 12 (2005)

and 14.1 (2007). Annual mean TSI exceeded 40 in 2005, and the value was 44.2. TP is the limiting nutrient based on a median TN/TP ratio of 33 (n=33).

09-2271 Wekiva River 3004J Lake Gandy Lake 3F Nutrients (TSI) median TN = 0.837 mg/L

(n=32), median TP =

0.02 mg/L

(n=32), median BOD=1.8 mg/L (n=20) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 35.7 (2006) and 51.3 (2007). Color value is 29.1 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 51.3. TP is the limiting nutrient based on a median TN/TP ratio of 38.9 (n=30).

09-2272 Wekiva River 3004K Lake Wekiva (Orlando) Lake 3F Nutrients (TSI) median TN = 1.4 mg/L (n=49), median TP = 0.0725 mg/L

(n=50), median BOD=4.6 mg/L (n=60) TSI < 60; Color > 40 3c 5 5 Impaired Medium Annual mean TSIs were 64.3

(2001), 63.9 (2002), 58.1 (2005), 61.9 (2006), and 64.0 (2007). Color value is

40 (2002). Annual mean TSI exceeded 60 in 2001- 2002 and 2006-2007, and the values were 64.3, 63.9, 61.9 and 64, respectively. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 19.9 (n=49).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 17 of 18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

09-2273 Wekiva River 3004N Lake Fairview

Lake Lake 3F Nutrients (TSI) median TN = 0.64 mg/L

(n=77), median TP = 0.015 mg/L

(n=70), median BOD=2 mg/L (n=69) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual mean TSIs were 31.9

(2001), 31.5 (2003), 44.7 (2005), and 42.2 (2007). Color values are 7.5 (2001) and 18 (2005). Annual mean TSI exceeded 40 in 2005, and the value was 44.7. TP is the limiting nutrient based on a median TN/TP ratio of 46.2 (n=68).

09-2274 Wekiva River 3004O Asher Lake Lake 3F Nutrients (TSI) median TN = 0.7335 mg/L

(n=31), median TP = 0.029 mg/L

(n=31), median BOD=2.4 mg/L (n=39) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 36.8 (2001), 53.4 (2002), 51.0 (2003), 56.2 (2004), and 45.3 (2007). Color value is 29.4 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 45.3. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 26.7 (n=31).

09-2275 Wekiva River 3004P Cub Lake Lake 3F Nutrients (TSI) median TN = 0.7066 mg/L

(n=48), median TP =

0.02 mg/L

(n=57), median BOD=2 mg/L (n=55) TSI < 40; Color < 40 2 5 5 Impaired Medium Annual means were 42.2 (1999), 36.2 (2001), 43.2 (2002), 35.8 (2003), and 40.4 (2007). Color value is 16.3 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 40.4. TP is the limiting nutrient based on a median TN/TP ratio of 40 (n=48).

09-2276 Wekiva River 3011A Lake Weston Lake 3F Nutrients (TSI) median TN = 0.804 mg/L

(n=23), median TP = 0.045 mg/L

(n=24), median BOD=3.3 mg/L (n=15) TSI < 40; Color < 40 3c 5 5 Impaired Medium Annual mean TSI was 56.4 (2007). Color value is 22.1 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 56.4. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 17.6 (n=23).

09-2277 Wekiva River 3011B Lake Shadow Lake 3F Nutrients (TSI) median TN = 0.554 mg/L

(n=21), median TP =

0.02 mg/L

(n=21), median BOD=2 mg/L (n=14) Annual average TSI >40 N/A 5 5 Impaired Medium Annual mean TSI was 46.3 (2007). Color value is 15.7 (2007). Annual mean TSI exceeded 40 in 2007, and the value was 46.3. TN and TP are co-limiting nutrients based on a median TN/TP ratio of 31.43 (n=21).

### Middle St Johns Basin Group 2 - Verified List - (Cycle 2 Revised and Readopted May 2009)

May 15, 2009 18 of 18 Florida Department of Environmental Protection

OGC Case Number Planning Unit WBID Water Segment

Name Waterbody Type Waterbody Class1 1998 303(d) Param. Of Concern Parameters Assessed Using the Impaired Surface Waters Rule (IWR) Dissolved Oxygen/Biology

Pollutant of

Concern DO / Nutrient / Biology - TN, TP, BOD Median Values (mg/L)2 Concentration of Criterion or Threshold Not Met EPA Integrated Report Category†-

Cycle 1

Assessment4 EPA Integrated Report Category†-

Cycle 2

Assessment5 Current Integrated Category - Final

Assessment Current Assessment Status Priority for TMDL Development6 Verified Period (# of Exceedances / # of Samples)8 Comments

1Florida's waterbody classifications are defined as: 1 - Potable water supplies 2 - Shellfish propagation or harvesting 3F - Recreation, propagation, and maintenance of a healthy, well-balanced population of fish and wildlife in fresh water 3M - Recreation, propagation, and maintenance of a healthy, well-balanced population of fish and wildlife in marine water 4 - Agricultural water supplies 5 - Navigation, utility, and industrial use

2n is equal to the number of samples. When samples are collected at the same location less than 4 days apart, the median of those results represents a single sample for the purpose of determining n.

3The period of record assessment refers to an evaluation of all data available for a particular waterbody and parameter not just the data within the Verified Period.

4The Cycle 1 assessment was done in 2003 and included data from that Verified Period (January 1, 1996 through June 30, 2003).

5The Cycle 2 assessment is the current assessment and includes data from the Verified Period (January 1, 2001 through June 30, 2008).

†EPA's Integrated Report Category: 1 - Attains all designated uses 2 - Attains some designated uses and insufficient or no information or data are present to determine if remaining uses are attained 3A - No data and information are present to determine if any designated use is attained 3B - Some data and informationare present but not enough to determine if any designated use is attained 3C - Enough data and information are present to determine that one or more designated uses may not be attained according to the Planning List methodology 3D - Enough data and information are present to determine that one or more designated uses are not attained according to the Verified List methodology 4A - Impaired for one or more designated uses but does not require TMDL development because a TMDL has already been completed. 4B - Impaired for one or more designated uses but does not require TMDL development because the water will attain water quality standards due to existing or porposed measures. 4C - Impaired for one or more criteria or designated uses but does not require TMDL development because impairment is not caused by a pollutant. 4D - The waterbody does not meet applicable criteria, but no pollutant can be identified; therefore a TMDL will not be developed at this time. 4E - Impaired, but recently completed or on-going restoration activites are underway to restore the designated uses of the waterbody. 5 - Water quality standards are not attained and a TMDL is required.

6Where a parameter was identified as impaired, a priority of “medium” was assigned. Exceptions are waters where the impairment poses a threat to potable water or human health, which have been assigned a “high” priority, and fecal coliform impairments, which have been assigned a “low” priority. All other listings as of this cycle are prioritized based on the following: it is our intent that listings with a "High" priority be addressed within the next 5 years, listings with a "Medium" priority be addressed within 5-10 years as resources allow, and listings with a "Low" priority be addressed within the next 10 years.

7PP- Planning Period (January 1, 1996 through December 30, 2005);

8VP- Verified Period (January 1, 2001 through June 30, 2008)

*A statewide TMDL for mercury, that will address this waterbody, is scheduled to be completed in 2012. ^ Beach advisories are based on FL Dept of Health Enterococcus (>103 CFU/100mL) or fecal coliform (>399 CFU/100mL) criteria. Fish advisory data is based on "2007 Fish Advisories" file created 07/11/2006 and updated 11/05/2007 by Barbara Donner of FDEP. The Group 2 Middle St Johns Master list is based on IWR Run 35.