15-Jun -12 Tes t T y p e l(cport # I T es t Syste m I Co mm e nt s

nc s ult s <:o mpl c t c cl S tut lics GLP: T h e
r a t oral LDSO was gre at er t han 5000 **mg/k~** ( l i m i t dose)
.There.: were no ucuths Acu t e Om l (LD50)

R ut Guidelinedu r ing t he exposu r e r ecovery pe r iod . O LP : The male and fema l e ra t ,whok

· body inha l u t i on 4 - hr LCSO wus g rcmcr t h an 1 5,/J()( Acute Inha l ation (4-hr LC50)
Rat ,whole· Not prm ( t he **i~hest**achievable vapor concen t ration)
.There were.: no dc:llhs durin g t he exposure body G uidclm c or recovery period. Anes t he t ic eiTects were observed at concentrations greater than or Ctlu:ll to 9700 ppm Acute Dcm1ul (L.D50)

RatOLI'. The rat uennal L D50 was greater than 5000 m glkg ( I unit dosel
.There were no Guide lin e **de<~ths** during the exposure recovery period
. Derma l Irritation RabbitGLP : was not a dermal irritant when test ed in rabbits
.No cllmcal s o gns of tox o cny was Guideline observed 10 the study
. Dermal Sensotmuion Mouse GLP, was not a dermal sensitizer when tested in mice .No ell meal Signs of tox1c11 y W(IS Gutdcllne observed 10 the study
. Eye Irritation RabbitGU' ,was not an eye irritant when tested in rabb1ts
.No cllnocal s1gns of t oxicny was Gu 1d clinc observed in the study
. 01.1' ; was not mtllagenic in the Bacteria
l Reverse Mutation Assay (Amcs) IC SI usmg **13ac~enal**Reverse Mutm ion A=y (Ames) l3actcria Salmonella strains TA98, TA I 00, TA 1 535, and TA 1537 and E .coli Slrain WP2 11vr A en her G uidelin e i n the presence o r absence of an S9 - activated test system. C hine se G l.l ':did not prod u ce st r uc t u r al o r numerica
l chromosome ull erra t ions in an in vttro I n Vnro C homosom e Aberration hamster ovury G uidclmcmammalian chromosome aberra t ion test us i ng Chinese h :unstcr ovnry (Cl i O) ce ll s either i n !Cl i O) ce ll s

t he presence or ahse n ce of an S9 - a c t r vated t est sys t em . Ma l e and female rats were expose d 1 0 a s i ngle ora l dose ofO ,500 ,1000 ,or 2000 m g/ kg I n Vivo Micro nu cle u s

RHIGLP ;

.N o c l inical sig n s o f tox i c it y or morta l ity was observed

i n th e st u dy .IIFX

· I IO did no t Guidel in e in duce b iologi c ally re l evant i nc r eases m micronuckalcd rctic u i OC)
' Ie s in pcr i p h cml blood ce ll s. M ale and lcmale rats we r e exposed

1 0 0, 250, 500 or I 000 m glkg
v i a oral gavngc 28-Day Oral

Rm GLP, for 28 days .No test substance-related adverse effects were observed in c l imca l or anato r mcu l Gmde lin c pathology assessmen
t s. The n o · observed
- adver se-eflcc tlcvcl (NOAEL) was 1000 m gl k g/ day .

i Male and female rats were exposed to 0 ,I 00, 250, or 1 000 mg/kg vta ora l g:rvagc

I 28 - Day O ral w ith FOB

' ' '
R:uGLP. for 28 days .No test substance-related adverse effects were observed in c l imca l .

, Ciuidc l tnc ncurobehav1oral, or anatomica

l pathology assessments Tho; n o-obscrved-adverse-ellcc

t l evel (NOAEI.) was 1000 mglkglday. Page 1 o f 3Male and female rat s were exposed to 0, 250, 500 or I 000 mglkg v ta oral gavage
for\)()

uay s. N o t es t subs tance - relat ed adverse e fli:cts were obse r ve d in clinica l

,

90-Day Ora l wllh FOO GLP ; ncurob c hn viora l

, or anatom i cal pathology

**sses~ mcnt** **s** tn females

. A slight in c rea se m

Ra

t

Gutde l ine se ver i ty , but nm i n ci dence

, of c hroni c progres s iv e nc l l h mpa t hy (CPN) a n d associa t e d clintcal

patho

l ogy changes was observed in ma l e s at nil do se l eve l s The no - obscrved - adverse -c l'r i.: c t

level

(N OAEL) for fema le rats was 1000 mg / k g f ua y . The NOAJ::L was not determin e d f or

i

m:Jic rats .

Pre -n atal Ora l Developmental I Fema l e rats were exposed t o 0, 250, 500, or I 000 m g( kg per da y via ora l gavage



### Rot



### GI.P ; during ges t a tion da y (G D ) 6·20 No t es t subs tan ce -r e l a t ed ma t ern al or developmentallox ic t tlGuideline was ob served a t any dose level. The no-observed -adve r se - effect level (NOAEL) for maternal

•

and fetal effects was 1000 mglkg.

Intra venous To lernbil it) ' S tu dy I Not GLP ; Male and female rats we re exposed t o to a single tnt ravenous tnjection of 0. I

, or 5 mwl.g

Rat

No t No deaths, clinical signs, or body weight losses occurred man y anima l after -1GUideline

days of observation

.

Ready

Oiodegradahili t y th) NA GLP;

**i~** not r cad t ly biotlcgradable

. No transformation prod u c t was ge neraled

**un d~r**  the

G

Uideli ne co nd i li o n s of th e t es t.

i Medaka were exp osed to 0.005 or 0

. 05 mg/L i n a co nl i nuo u s llow-l h ro u gh dt l u t i on

syste

m for 60 days followed by a 1 4-day dcpurmion period

. The steady-s late OCr va l ue s

Bioconcen t ration S t u d y ' " 1 Fish GLP ; ranged from II 00 10 22l0 forthe 0.05 mg(L l eve l and fr o m 1560 to 2850 fo r the 0.005 rn g(LGuideline

l evel. T h e co nc e ntration of in lh e fi s h d ec r eased with time during t h e depuration
periotl . The biological half - l iv es ranged from 3

. 1 t o 6

. 6 days for the 0.05 mgfL l eve l and fmn

2 . 5 t o 4

. 7 days forthe 0

. 005 m gfL le ve l Average BCF for 12 datapoims = 1990± 46 0

.

The green algae 72-h r EsC50 was greater th an 0.00().1 77 mg!L (the highestconcentrnl!on

l ested)

. The no observed em:ct concentration (NOEC r ) al 72 hours was

Algae Growth l n hthtll on Tcst lhl Gree n algae GLP ; 0

. 000477 mg/L ( th e h ighes t dose les t ed)

. The conce nt ra l ion was the t ime-weigh t ed m ean of

G

uidelin e th e limil o f so l ubi lil y (0

. 16 mg!L) after 72-hour s . During t he course oflhe study

, il wasdclennm

ed !hut the test subs1ance was volutilt zc d fr o m the rest so lu t ions due to a l arge

l

lenry 's law cons tan t of 4. 75E6 n h mol

.

Algae Growt h Inhibit i on Tes t No t GLP ; The gree n algae 72-hr ErC50 wu s greater I han 1 2 0 mg(L (t h e hi ghes t

**conc~ nt** **nltion**

Gree n algae No t Guide

l ine le st cd )

.

fish Acute Toxtclly Tes t 1 " 1 GLP; The Medakn 96-hr LCSO was grea t er !han 0

. 0

. 960 mgfL ( lhe highe s t c o nce111m11 on
Mcdaka Guideline te ste d ) The co n cen tmt io n was the tim e-weig ht ed mean of th e limit of solubi l ity (0

. 1 mg / L)

after

96-hours

. The t es t solution was r enewed every 24 hours

.

F is h Acute Toxicity Tes t Fathead Not GLP

, The fathead minnow 96 -hr LC50 was greater than 120 mg(L ( th e highe st

Not

Minnow

Gui delin e concentra t ion tested)

.
Daphnia Acute Immobilization Test 

# ' '' l Da phn ia GLP; The Daphnia magna 48 - hr ECSO wa s grea 1 cr th an 0

. 157 m g! L ( the highes t concentral

t O n IC S t e d). The concenlration was th e t u ne- w eigh l e d mea n oflhe l i mit of

ma gna G uid elineso

l ubi l ily (0

. 17 mg / L) after 48 - hour s . The lest so luu o n wa s renewed afler 2 4 hours.

Daphnia Acut e lmmoh t lo za t ion T est Daphnia No1 GLP ; The Daphma magna 48 - hr ECSO was grea1cr than 120 mg!L ( t he highe s t



### N otma

g na G u ide l ine con cc n t ra t ion t csled )

.

Act iva t e d S lud ge Respiration ln hibi 11on Te st Mtcrobtal GLP

, The microbial resp iralion inh ibi tton ECSO wa s grea ter t han 10 00 p pm (highes t doseGuideline

tested)

. No inhibition was noted at a n y co nc ent ra tio n . Page 2 of3

## The s tu dy wa s cond u c t ed u sing t he I IPL C Method fo r de t erm ining a su b s tance's absorptin n

Adsorption

Cocnlcicn t (Log K oc) NA GLP: coefftcicn t (so il organic

**c~rbon wa** **)** 

## on s oil and se wag e s lud g e . The adsorption cocl'licil: n tGuidelin.:

(log Koc) v a lu e of the te s t

**ub ancc**  

## w a s ca l c ul a t ed t o be 4

. 50 (l n so t I and 4 .43 o n so.:\\ age
sludgeKOWW IN vl.68 was use d t o t:Stltnnte LogKow

. components ta iled to sati>Y the

LogKow (mode l led) Not GL P

, training se t ' s s tru ctura l doma in req 111 rcments complete l y, however, othe r evtdc n ce by way of

NA No t r elat ed analog ue s and wa t er so lu bi l ity infon n at i on pr ov id e corroborat ing ev i dence t h at t he

G uid e l ine es ti ma t es made by t he m ode l arc r e a so nab l e . LogKo w predic tiO n rang ed from 4

. 57 t o 5

. 22

for

A we i g h te d avera sc ha s ed on t he t y pt cal co mponcn1 percen t ages and the i r
estima

t e d LogKow values wa s cal c ulat e d t o be 

## approximate l y 4.97

. On-J,!o in g St ut l il 'S ll F X'- 1 1 0-----

------ ---


## Daphnia R e p roductio n Tes t t•l Daphma

Guideline in prog r ess, r e110r l CX I ICCied I S, Junc

mag n a

Ze bra fi s h 1 4 - da y G r o wth S t u d y« > 7.cb rafi s h Guidelme i n r trog r · css, rcp o rl eX I lCC t c d e nd of l\l:1y

E t SCilHl 

## T he E is enoa foc t ida 14-dny L C50 was gr ea t e r t h an 1 00 0 mglkg d ry soi l ( t he l ughc s t

E

anh worm A c ute Toxici t y Tes t <• > f oetidu Guidel i ne a n d on l y conce ntration te ste d )

in

l>rOJ.!r CSS, reuo rt ex peeled e n d or 1\'lny
GL P ; ts inheren tl y biod egmdablc. Biodegmdation was 44 J% 

## afie r 28 days under the

Inherent

B i odcgmdabtli t ) ' Tes t 1 d 1 

## NA

Guideline conditions of the test .in**pr o~:ress,** 



## rcporl ex pee l ed end of 1\l:ty

No t GLP ;

T

ox i c okin e t ics Te s t 1 "' 

## Rut N o t p cnt l l n gGuideline LogKow (measu r e d) < h i NA G Uidel i ne l lCn<ling 

# Page 3 of 3