# Patent

## Title
Rabies virus-based recombinant immunocontraceptive compositions and methods of use

## Abstract

Described herein are recombinant rabies viruses comprising a heterologous nucleic acid sequence encoding an immunocontraceptive protein, such as gonadotropin-releasing hormone (GnRH) or zona pellucida 3 (ZP3). The recombinant rabies viruses disclosed herein are recovered by reverse genetics, replicate efficiently, elicit rabies virus neutralizing antibodies and immunocontraceptive peptide-specific antibodies in vaccinated animals, and protect vaccinated animals against wild-type rabies virus challenge. Further provided is a method of immunizing a non-human animal against rabies virus infection and simultaneously inhibiting fertility of the animal, comprising administering an immunogenic composition comprising one or more of the recombinant rabies viruses described herein.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This is the U.S. National Stage of International Application No. PCT/US2009/054502, filed Aug. 20, 2009, which was published in English under PCT Article 21(2), which in turn claims the benefit of U.S. Provisional Application No. 61/097,748, filed Sep. 17, 2008, which is herein incorporated by reference in its entirety.


FIELD
This disclosure concerns recombinant rabies viruses as immunocontraceptive compositions for control of wild and domestic animal population growth, as well as protection of animals against rabies virus infection.
BACKGROUND
Rabies is a major threat to public health, causing between 50,000 and 60,000 human deaths each year (World Health Organization, April 2003). Humans get infected with the rabies virus mostly through bites from rabid domestic and wildlife animals. In developing countries, dogs are responsible for about 94% of human rabies deaths. Dog rabies is still epizootic in most countries of Africa, Asia and South America, and in these countries dogs are responsible for most human deaths from the disease. Controlling rabies virus infection in domestic and wildlife animals, therefore, not only reduces the mortality in these animals but also reduces the risks of human exposure.
The rabies virus is transmitted through broken skin by the bite or scratch of an infected animal. Exposure to rabies virus results in its penetration of peripheral, unmyelinated nerve endings, followed by spreading through retrograde axonal transport, replication occurring exclusively in the neurons, and finally arrival in the central nervous system (CNS). Infection of the CNS causes cellular dysfunction and death (Rupprecht and Dietzschold, Lab Invest. 57:603, 1987). Since rabies virus spreads directly from cell to cell, it largely evades immune recognition (Clark and Prabhakar, Rabies, In: Olson et al., eds., “Comparative Pathology of Viral Disease,” 2:165, Boca Raton, Fla., CRC Press, 1985).
Population control of dogs with outdated methods of capture, restraint and euthanasia are inhumane and not acceptable to the public. Canine rabies prevention and control, and appropriate population management of free-ranging dogs are paramount for eventual disease elimination. Various approaches have been proposed to interrupt canine reproductive cycles, including surgical spay/neuter of animals, chemical sterilization, and immunocontraception. For example, gonadotropin releasing hormone (GnRH) has been considered as one approach as an immunocontraceptive peptide for dogs. However, studies to date have shown that GnRH needs to be synthesized and conjugated with a carrier protein (or adjuvant) to be immunogenic. Necessary scale-up of production may become problematic to meet the regulatory and economic demands for modern vaccine supply. Thus, it is desirable to construct a vaccine that can induce appropriate dual immunological responses against both rabies virus and immunocontraceptive targets, after a single administration in animals.
Moreover, over the past 30 years, immunocontraceptive studies have not generated a single commercial product. Technical limitations are one of the main factors. Therefore, there is a long unfelt need for a novel rabies virus vaccine, engineered with the ability to express a suitable immunocontraceptive gene. This type of vaccine would be an ideal candidate for both rabies prevention and population control of wild and domestic animals, including dogs.
SUMMARY OF THE DISCLOSURE
Recombinant rabies viruses comprising heterologous nucleic acid sequences encoding immunocontraceptive proteins are disclosed herein. The recombinant rabies viruses are recovered using reverse genetics, replicate efficiently in culture, and elicit high titers of rabies virus neutralizing antibodies, elicit immunocontraceptive protein-specific antibodies and confer protection against rabies virus challenge in vaccinated animals.
Provided herein is a recombinant rabies virus in which the genome of the recombinant rabies virus includes a heterologous nucleic acid sequence encoding an immunocontraceptive protein. In some embodiments, the immunocontraceptive protein is gonadotropin-releasing hormone (GnRH) or zona pellucida 3 (ZP3), such as dog ZP3. In some embodiments, the genome of the recombinant rabies virus comprises a nucleic acid sequence encoding ZP3 and a nucleic acid sequence encoding GnRH.
Also provided are immunogenic compositions comprising one or more of the recombinant rabies viruses described herein. Further provided is an immunogenic composition comprising a first recombinant rabies virus and a second recombinant rabies virus, wherein the genome of the first recombinant rabies virus comprises a GnRH nucleic acid sequence and the genome of the second recombinant rabies virus comprises a ZP3 nucleic acid sequence.
Further provided are methods of immunizing a non-human animal against rabies virus infection and inhibiting fertility of the animal, by administering to the animal a therapeutically effective amount of an immunogenic composition comprising one or more of the recombinant rabies viruses disclosed herein.
The foregoing and other features and advantages will become more apparent from the following detailed description of several embodiments, which proceeds with reference to the accompanying figures.



BRIEF DESCRIPTION OF THE FIGURES
FIG. 1 is a schematic depiction of four recombinant ERAZP3 viruses. G* denotes the mutation at amino acid 333 of glycoprotein (G). ZP—indicates a dog zona pellucida gene.
FIG. 2A is a schematic depiction of the rabies virus glycoprotein. Arrows indicate locations where either one or two copies of GnRH were inserted. Recombinant viruses with GnRH inserted at each of these locations were successfully recovered by reverse genetics (Ecto=ectodomain; SP=signal peptide; TM=transmembrane; IIb, II, IIa, WB+ and III refer to antigenic sites). FIG. 2B is a schematic depiction of recombinant rabies virus ERA-3-GnRH.
FIG. 3A is a table listing exemplary recombinant rabies viruses comprising dog ZP3 (DZP3), GnRH or both. The virus descriptions indicate the location of insertion of ZP3 and/or GnRH in the virus genome (G3=glycoprotein with the G333 mutation). FIG. 3B is a graph showing survival of unvaccinated mice (control) or mice vaccinated with either ERA-N-GnRH (virus #5), ERA-3-GnRH (virus #7) or ERA-G3-2GnRH (virus #8). Each group of mice was subsequently challenged with a lethal dose of rabies virus.
FIG. 4 is an image of a protein gel showing GnRH or 2GnRH peptide conjugated to keyhole limpet hemocyanin (KLH). The proteins were separated on 4-12% SDS-PAGE gels. GnRH-KLH and 2GnRH-KLH are shown in lanes 2 and 4, respectively. Lanes 1 and 6 contain molecular weight markers. Lanes 3 and 5 show KLH standard.
FIG. 5A is a schematic of the parental ERA and rearranged ERAg3p genomes. To generate ERAg3p, the G gene in the ERA genome was relocated ahead of the P gene, and was mutated at amino acid residue 333 from AGA (denoted as G) to GAG (denoted as G*). FIG. 5B is a one-step growth curve showing growth characteristics of the rearranged ERAg3p virus. The recovered virus ERAg3p grew as well as the parental ERA virus. FIG. 5C is a line graph comparing virulence of ERA and ERAg3p. ERAg3p did not cause death in any 3-week old mice after intracerebral injection.
FIG. 6 is a schematic showing insertion sites of GnRH or 2GnRH coding sequence into the G gene in ERAg3p rabies virus. SP=signal peptide; TM=transmembrane; CT=cytoplasmic tail; N=amino terminus of glycoprotein; and C=carboxyl-terminus of glycoprotein.
FIG. 7A is a schematic showing insertion sites of GnRH into the ERAg3p genome to generate ERA-N-GnRH, ERA-N-2GnRH, ERA-IIa-GnRH and ERA-C-GnRH. FIG. 7B is a line graph showing recovery and growth characteristics of the GnRH-carrying ERAg3p viruses. Recombinant virus was successfully recovered from 4 out of the 12 constructs. Recovered viruses contained GnRH inserted at the amino terminus immediate after the signal sequence, the IIa antigenic site, or the junction between the ectodomain and transmembrane domain of glycoprotein.
FIG. 8A is an image of an electrophoretic gel showing purified ERA-N-2GnRH (lane 1), ERA-N-GnRH (lane 2) and ERA-IIa-GnRH (lane 3). Purified virus was separated on 4-12% SDS-PAGE gels. Lanes 4 and 5 contain purified glycoprotein and purified nucleoprotein from rabies virus ERA as controls. FIG. 8B is an image of a Northern blot of purified ERA-N-2GnRH (lane 2) and ERA-N-GnRH (lane 3). Lanes 1 and 4 contain RNA molecular weight marker.
FIG. 9 is a line graph showing safety and potency of the GnRH-carrying ERAg3p viruses in a mouse model. No obvious side-effects were observed after intramuscular injection of ERA-N-2GnRH, ERA-N-GnRH or ERA-IIa-GnRH in mice. Three weeks post-inoculation, all mice survived challenge with a lethal dose of approximately 2.5-10.0 MICLD50 dog/coyote street rabies virus. The control mice (placebo injected) died between 8 and 10 days after challenge. The surviving mice remained healthy before termination of the experiment at 2 months.
FIGS. 10A-10D are Western blots showing reaction of GnRH-KLH and 2GnRH-KLH conjugates against mouse serum immunized with GnRH-carrying ERA viruses and GonaConTM serum. For each blot, Lanes 1 and 2 contain GnRH-KLH and 2GnRH-KLH, respectively. Shown are mouse serum from rabies virus ERA-IIa-GnRH immunization (A); mouse serum from RV ERA-N-GnRH immunization (B); mouse serum from ERA-N-2GnRH immunization (C); and rabbit serum against GonaConTM (D). No differences were detected between mouse and rabbit serum against the GnRH conjugates.



SEQUENCE LISTING
The nucleic and amino acid sequences listed in the accompanying sequence listing are shown using standard letter abbreviations for nucleotide bases, and three letter code for amino acids, as defined in 37 C.F.R. 1.822. Only one strand of each nucleic acid sequence is shown, but the complementary strand is understood as included by any reference to the displayed strand. The Sequence Listing is submitted as an ASCII text file, created on Feb. 28, 2011, 158 KB, which is incorporated by reference herein. In the accompanying sequence listing:
SEQ ID NO: 1 is the nucleotide sequence of recombinant rabies virus ERA recovered by reverse genetics. Mutation of nucleotides 4370-4372 from aga to gag introduces an Arg to Glu amino acid change in the G protein.
SEQ ID NO: 2 is the amino acid sequence of the rabies virus ERA N protein.
SEQ ID NO: 3 is the amino acid sequence of the rabies virus ERA P protein.
SEQ ID NO: 4 is the amino acid sequence of the rabies virus ERA M protein.
SEQ ID NO: 5 is the amino acid sequence of the rabies virus ERA G protein. An Arg to Glu change at amino acid residue 352 is an attenuating mutation.
SEQ ID NO: 6 is the amino acid sequence of the rabies virus ERA L protein.
SEQ ID NOs: 7 and 8 are the nucleotide and amino acid sequences, respectively, of dog zona pellucida 3 (ZP3).
SEQ ID NOs: 9-26 are the nucleotide sequences of the oligonucleotides use to generate fragment A of dog ZP3.
SEQ ID NOs: 27-46 are nucleotide sequences of the oligonucleotides used to generate fragment B of dog ZP3.
SEQ ID NOs: 47 and 48 are the nucleotide and amino acid sequences, respectively, of GnRH.
SEQ ID NOs: 49 and 50 are the nucleotide and amino acid sequences, respectively, of rabies virus ERA G protein with a single copy of GnRH inserted immediately following the 19 amino acid G protein signal sequence. This construct is referred to as G-N-GnRH.
SEQ ID NOs: 51 and 52 are the nucleotide and amino acid sequences, respectively, of rabies virus ERA G protein with two copies of GnRH inserted immediately following the 19 amino acid G protein signal sequence. This construct is referred to as G-N-2GnRH.
SEQ ID NOs: 53 and 54 are the nucleotide and amino acid sequences, respectively, of rabies virus ERA G protein with a single copy of GnRH inserted immediately following amino acid 221 of the G protein (IIa site). This construct is referred to as GnRH-p3 or G-IIa-GnRH.
SEQ ID NO: 55 is the amino acid sequence of GnRH peptide 1780.
SEQ ID NO: 56 is the amino acid sequence of GnRH peptide 1781.
SEQ ID NO: 57 is the nucleotide sequence of 2GnRH (two tandem copies of the GnRH coding sequence).
SEQ ID NOs: 58 and 59 are the nucleotide sequences of primers used for insertion of the GnRH coding sequence into the rabies virus G gene.
SEQ ID NOs: 60 and 61 are the nucleotide sequences of primers used for insertion of the tandem GnRH (2GnRH) coding sequence into the rabies virus G gene.
SEQ ID NO: 62 is the nucleotide sequence of dog ZP3, deposited under GenBank Accession No. NM—001003224 on Aug. 5, 2004.
SEQ ID NOs: 63 and 64 are the nucleotide and amino acid sequences, respectively, of rabies virus ERA G protein with one copy of GnRH inserted at the junction of the ectodomain and the transmembrane domain (following nucleotide 1374, amino acid 458) of glycoprotein. This construct is referred to as G-C-GnRH.
DETAILED DESCRIPTION
I. Introduction
Rabies is a major public health concern globally. In most instances, humans are infected with rabies virus through the bite of a rabid domestic or wild animal. In developing countries, dogs are responsible for approximately 94% of human deaths due to rabies. Stray or unvaccinated dogs are the primary reservoir for rabies in Latin American, Asian and African countries. Furthermore, in the United States, there are currently millions of stray or feral cats. Thus, there is a global need to both prevent rabies and control the population of rabies susceptible animals, particularly dogs.
Previous methods of animal population control have included the use of immunocontraceptive vaccines. Immunocontraception involves stimulating immune responses against gametes or reproductive hormones to prevent conception. Immunocontraception is a humane method for population control of pest and overabundant populations of mammalian wildlife (such as raccoons or deer). A number of studies have focused on the use of zona pellucida glycoprotein 3 (ZP3), which is the main receptor used by sperm for fertilization of an egg. However, administration of ZP3, or other immunocontraceptive protein, has previously required co-administration of an adjuvant and/or booster doses to elicit a sufficient immune response against the protein such that fertilization is inhibited. Thus, current methods of immunocontraception have significant limitations, particularly for wild animal populations.
The immunogenic compositions and methods disclosed herein provide a means of simultaneously protecting vaccinated animals against rabies and controlling animal populations by inhibiting fertility. Recombinant rabies viruses comprising at least one heterologous nucleic acid sequence encoding an immunocontraceptive protein are described herein. In particular examples, the immunocontraceptive protein is GnRH or ZP3. In some cases, the recombinant rabies viruses comprise both GnRH and ZP3. Alternatively, animals can be immunized with two different recombinant rabies viruses, one comprising GnRH, and a second comprising ZP3. Because the immunocontraceptive protein is encoded in the genome of the rabies virus, when recombinant rabies virus particles are produced, the immunocontraceptive peptides are incorporated into the virion (structural protein) or are contained within the virion (non-structural protein). By incorporating the immunocontraceptive protein into the rabies virus particle, an adjuvant is not required to elicit a sufficient immune response against both rabies virus and the immunocontraceptive protein.
II. Abbreviations
















CMV
Cytomegalovirus



CTVT
Canine transmissible venereal tumor



DFA
Direct fluorescent assay



DNA
Deoxyribonucleic acid



ERA
Evelyn-Rokitnicki-Abelseth



FFU
Focus-forming units



FITC
Fluorescein isothiocyanate



FSH
Follicle stimulating hormone



G
Rabies virus glycoprotein



G*
Glycoprotein with an Arg to Glu change at residue 333



GnRH
Gonadotropin-releasing hormone



HPLC
High performance liquid chromatography



i.c.
Intracerebral



i.m.
Intramuscular



IRES
Internal ribosome entry site



KLH
Keyhole limpet hemocyanin



L
Rabies virus RNA-dependent RNA polymerase



LH
Luteinizing hormone



M
Rabies virus matrix protein



MALDI
Matrix-assisted laser desorption/ionization



MICLD50
Mouse intracerebral lethal dose 50



N
Rabies virus nucleoprotein



NA
Neutralizing antibody



NLS
Nuclear localization signal



P
Rabies virus phosphoprotein



PAGE
Polyacrylamide gel electrophoresis



PVDF
Polyvinylidene diflouride



pZP
Porcine zona pellucida



RNA
Ribonucleic acid



RNP
Ribonucleoprotein



RV
Rabies virus



SDS
Sodium dodecyl sulfate



UV
Ultraviolet



VNA
Virus neutralizing antibody



ZP
Zona pellucida










III. Terms
Unless otherwise noted, technical terms are used according to conventional usage. Definitions of common terms in molecular biology may be found in Benjamin Lewin, Genes V, published by Oxford University Press, 1994 (ISBN 0-19-854287-9); Kendrew et al. (eds.), The Encyclopedia of Molecular Biology, published by Blackwell Science Ltd., 1994 (ISBN 0-632-02182-9); and Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, published by VCH Publishers, Inc., 1995 (ISBN 1-56081-569-8).
In order to facilitate review of the various embodiments of the disclosure, the following explanations of specific terms are provided:
Adjuvant: A substance or vehicle that non-specifically enhances the immune response to an antigen. Adjuvants can include a suspension of minerals (alum, aluminum hydroxide, or phosphate) on which antigen is adsorbed; or water-in-oil emulsion in which antigen solution is emulsified in mineral oil (for example, Freund's incomplete adjuvant), sometimes with the inclusion of killed mycobacteria (Freund's complete adjuvant) to further enhance antigenicity. Immunostimulatory oligonucleotides (such as those including a CpG motif) can also be used as adjuvants (for example, see U.S. Pat. Nos. 6,194,388; 6,207,646; 6,214,806; 6,218,371; 6,239,116; 6,339,068; 6,406,705; and 6,429,199). Adjuvants also include biological molecules, such as costimulatory molecules. Exemplary biological adjuvants include IL-2, RANTES, GM-CSF, TNF-α, IFN-γ, G-CSF, LFA-3, CD72, B7-1, B7-2, OX-40L and 41 BBL.
Administer: As used herein, administering a composition to a subject means to give, apply or bring the composition into contact with the subject. Administration can be accomplished by any of a number of routes, such as, for example, topical, oral, subcutaneous, intramuscular, intraperitoneal, intravenous, intrathecal and intramuscular. In some embodiments described herein, an immunogenic composition is administered to an animal by an oral route.
Animal: Living multi-cellular vertebrate organisms, a category that includes, for example, mammals and birds. The term mammal includes both human and non-human mammals. The term “animal” includes both human and veterinary subjects, for example, humans, non-human primates, dogs, cats, horses, raccoons, bats, rats, mice, foxes, squirrels, opossum, coyotes, wolves and cows. As used herein, “subject” is interchangeable with “animal.” As used herein a “domestic animal” refers to any animal that has been tamed by humans, often for use as work animals, a food source or as pets. Many domestic animals are selectively bred such that they differ from animals in the wild. As used herein, “wild animal” refers any animal living in a natural, undomesticated state.
Antibody: A protein (or protein complex) that includes one or more polypeptides substantially encoded by immunoglobulin genes or fragments of immunoglobulin genes. The recognized immunoglobulin genes include the kappa, lambda, alpha, gamma, delta, epsilon, and mu constant region genes, as well as the myriad immunoglobulin variable region genes. Light chains are classified as either kappa or lambda. Heavy chains are classified as gamma, mu, alpha, delta, or epsilon, which in turn define the immunoglobulin classes, IgG, IgM, IgA, IgD and IgE, respectively.
The basic immunoglobulin (antibody) structural unit is generally a tetramer. Each tetramer is composed of two identical pairs of polypeptide chains, each pair having one “light” (about 25 kDa) and one “heavy” (about 50-70 kDa) chain. The N-terminus of each chain defines a variable region of about 100 to 110 or more amino acids primarily responsible for antigen recognition. The terms “variable light chain” (VL) and “variable heavy chain” (VH) refer, respectively, to these light and heavy chains.
As used herein, the term “antibody” includes intact immunoglobulins as well as a number of well-characterized fragments. For instance, Fabs, Fvs, and single-chain Fvs (SCFvs) that bind to target protein (or epitope within a protein or fusion protein) would also be specific binding agents for that protein (or epitope). These antibody fragments are as follows: (1) Fab, the fragment which contains a monovalent antigen-binding fragment of an antibody molecule produced by digestion of whole antibody with the enzyme papain to yield an intact light chain and a portion of one heavy chain; (2) Fab′, the fragment of an antibody molecule obtained by treating whole antibody with pepsin, followed by reduction, to yield an intact light chain and a portion of the heavy chain; two Fab′ fragments are obtained per antibody molecule; (3) (Fab′)2, the fragment of the antibody obtained by treating whole antibody with the enzyme pepsin without subsequent reduction; (4) F(ab′)2, a dimer of two Fab′ fragments held together by two disulfide bonds; (5) Fv, a genetically engineered fragment containing the variable region of the light chain and the variable region of the heavy chain expressed as two chains; and (6) single chain antibody, a genetically engineered molecule containing the variable region of the light chain, the variable region of the heavy chain, linked by a suitable polypeptide linker as a genetically fused single chain molecule. Methods of making these fragments are routine (see, for example, Harlow and Lane, Using Antibodies: A Laboratory Manual, CSHL, New York, 1999).
Antibodies for use in the methods and compositions of this disclosure can be monoclonal or polyclonal. Merely by way of example, monoclonal antibodies can be prepared from murine hybridomas according to the classical method of Kohler and Milstein (Nature 256:495-97, 1975) or derivative methods thereof. Detailed procedures for monoclonal antibody production are described in Harlow and Lane, Using Antibodies: A Laboratory Manual, CSHL, New York, 1999.
Antibody binding affinity: The strength of binding between a single antibody binding site and a ligand (e.g., an antigen or epitope). The affinity of an antibody binding site X for a ligand Y is represented by the dissociation constant (Kd), which is the concentration of Y that is required to occupy half of the binding sites of X present in a solution. A smaller (Kd) indicates a stronger or higher-affinity interaction between X and Y and a lower concentration of ligand is needed to occupy the sites. In general, antibody binding affinity can be affected by the alteration, modification and/or substitution of one or more amino acids in the epitope recognized by the antibody paratope.
In one example, antibody binding affinity is measured by end-point titration in an Ag-ELISA assay. Antibody binding affinity is substantially lowered (or measurably reduced) by the modification and/or substitution of one or more amino acids in the epitope recognized by the antibody paratope if the end-point titer of a specific antibody for the modified/substituted epitope differs by at least 4-fold, such as at least 10-fold, at least 100-fold or greater, as compared to the unaltered epitope.
Antigen: A compound, composition, or substance that can stimulate the production of antibodies or a T-cell response in an animal, including compositions that are injected or absorbed into an animal. An antigen reacts with the products of specific humoral or cellular immunity, including those induced by heterologous immunogens.
Attenuated: In the context of a live virus, such as a rabies virus, the virus is attenuated if its ability to infect a cell or subject and/or its ability to produce disease is reduced (for example, eliminated). Typically, an attenuated virus retains at least some capacity to elicit an immune response following administration to an immunocompetent subject. In some cases, an attenuated virus is capable of eliciting a protective immune response without causing any signs or symptoms of infection.
cDNA (complementary DNA): A piece of DNA lacking internal, non-coding segments (introns) and regulatory sequences that determine transcription. cDNA is synthesized in the laboratory by reverse transcription from messenger RNA extracted from cells.
Epitope: An antigenic determinant. These are particular chemical groups, such as contiguous or non-contiguous peptide sequences, on a molecule that are antigenic, that is, that elicit a specific immune response. An antibody binds a particular antigenic epitope based on the three dimensional structure of the antibody and the matching (or cognate) three dimensional structure of the epitope.
Fertility: Refers to the ability of an animal to produce offspring. As used herein “inhibiting fertility” refers to reducing the rate of, or preventing, reproduction.
Fixed: A fixed rabies virus is a strain of rabies virus that has undergone serial passage in a host to stabilize virulence of the virus. Fixed rabies viruses include, but are not limited to CVS, ERA, PV, SAD-B19 and HEP-Flury strains (Anilionis et al., Nature 294:275-278, 1981; Morimoto et al., Viral. 173:465-477, 1989).
Fusion protein: A protein generated by expression of a nucleic acid sequence engineered from nucleic acid sequences encoding at least a portion of two different (heterologous) proteins. To create a fusion protein, the nucleic acid sequences must be in the same reading frame and contain to internal stop codons.
Gonadotropin-releasing hormone (GnRH): A peptide hormone responsible for the release of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the anterior pituitary. GnRH is synthesized and released by the hypothalamus and travels to the pituitary to mediate release of FSH and LH. The GnRH precursor protein is 92 amino acids and is processed to a decapeptide in mammals. GnRH is also known as GNRH1, luteinizing hormone releasing hormone (LHRH), progonadoliberin-1 and progonadoliberin-1 precursor. The term “GnRH” includes GnRH analogs and variants, including GnRH molecules containing substitutions, deletions, or insertions. The nucleotide and amino acid sequences of mammalian GnRH are set forth herein as SEQ ID NOs: 47 and 48, respectively.
Heterologous: As used herein, a “heterologous nucleic acid sequence” is a nucleic acid sequence that is derived from a different source or species. In some embodiments described herein, the heterologous nucleic acid sequence is a nucleic acid sequence encoding ZP3. In other embodiments, the heterologous nucleic acid sequence is a nucleic acid sequence encoding GnRH. In the context of a recombinant rabies virus, a heterologous nucleic acid sequence is any nucleic acid sequence that is not derived from the rabies virus.
Hybridization: Oligonucleotides and their analogs hybridize by hydrogen bonding, which includes Watson-Crick, Hoogsteen or reversed Hoogsteen hydrogen bonding, between complementary bases. Generally, nucleic acid consists of nitrogenous bases that are either pyrimidines (cytosine (C), uracil (U), and thymine (T)) or purines (adenine (A) and guanine (G)). These nitrogenous bases form hydrogen bonds between a pyrimidine and a purine, and the bonding of the pyrimidine to the purine is referred to as “base pairing.” More specifically, A will hydrogen bond to T or U, and G will bond to C. “Complementary” refers to the base pairing that occurs between to distinct nucleic acid sequences or two distinct regions of the same nucleic acid sequence.
“Specifically hybridizable” and “specifically complementary” are terms that indicate a sufficient degree of complementarity such that stable and specific binding occurs between the oligonucleotide (or its analog) and the DNA or RNA target. The oligonucleotide or oligonucleotide analog need not be 100% complementary to its target sequence to be specifically hybridizable. An oligonucleotide or analog is specifically hybridizable when binding of the oligonucleotide or analog to the target DNA or RNA molecule interferes with the normal function of the target DNA or RNA, and there is a sufficient degree of complementarity to avoid non-specific binding of the oligonucleotide or analog to non-target sequences under conditions where specific binding is desired, for example under physiological conditions in the case of in vivo assays or systems. Such binding is referred to as specific hybridization.
Hybridization conditions resulting in particular degrees of stringency will vary depending upon the nature of the hybridization method of choice and the composition and length of the hybridizing nucleic acid sequences. Generally, the temperature of hybridization and the ionic strength (especially the Na+ and/or Mg++ concentration) of the hybridization buffer will determine the stringency of hybridization, though wash times also influence stringency. Calculations regarding hybridization conditions required for attaining particular degrees of stringency are discussed by Sambrook et al. (ed.), Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989, chapters 9 and 11; and Ausubel et al. Short Protocols in Molecular Biology, 4th ed., John Wiley & Sons, Inc., 1999.
For purposes of the present disclosure, “stringent conditions” encompass conditions under which hybridization will only occur if there is less than 25% mismatch between the hybridization molecule and the target sequence. “Stringent conditions” may be broken down into particular levels of stringency for more precise definition. Thus, as used herein, “moderate stringency” conditions are those under which molecules with more than 25% sequence mismatch will not hybridize; conditions of “medium stringency” are those under which molecules with more than 15% mismatch will not hybridize, and conditions of “high stringency” are those under which sequences with more than 10% mismatch will not hybridize. Conditions of “very high stringency” are those under which sequences with more than 6% mismatch will not hybridize.
“Specific hybridization” refers to the binding, duplexing, or hybridizing of a molecule only or substantially only to a particular nucleotide sequence when that sequence is present in a complex mixture (for example, total cellular DNA or RNA). Specific hybridization may also occur under conditions of varying stringency.
Immune response: A response of a cell of the immune system, such as a B-cell, T-cell, macrophage or polymorphonucleocyte, to a stimulus such as an antigen. An immune response can include any cell of the body involved in a host defense response, including for example, an epithelial cell that secretes an interferon or a cytokine. An immune response includes, but is not limited to, an innate immune response or inflammation. As used herein, a protective immune response refers to an immune response that protects a subject from infection (prevents infection or prevents the development of disease associated with infection).
Immunize: To render a subject protected from a disease (for example, an infectious disease), such as by vaccination.
Immunocontraceptive protein: Refers to a protein or protein fragment (also referred to as an “antigen”) capable of eliciting an immune response in a subject that results in inhibition or loss of fertility in the subject.
Immunogen: A compound, composition, or substance which is capable, under appropriate conditions, of stimulating an immune response, such as the production of antibodies or a T-cell response in an animal, including compositions that are injected or absorbed into an animal.
Immunogenic composition: A term used herein to mean a composition useful for stimulating or eliciting a specific immune response (or immunogenic response) in a vertebrate. The immunogenic composition includes a recombinant rabies virus, such as a recombinant rabies virus expressing a heterologous protein (such as ZP3 and/or GnRH). In some embodiments, the immunogenic response is protective or provides protective immunity, in that it enables the vertebrate animal to better resist infection with or disease progression from the organism against which the immunogenic composition is directed (e.g., rabies virus). When the immunogenic compositions comprise an immunocontraceptive peptide, the immunogenic response elicited prevents or decreases the risk of pregnancy in female animals.
Without wishing to be bound by a specific theory, it is believed that an immunogenic response induced by an immunogenic composition may arise from the generation of an antibody specific to one or more of the epitopes provided in the immunogenic composition. Alternatively, the response may comprise a T-helper or cytotoxic cell-based response to one or more of the epitopes provided in the immunogenic composition. All three of these responses may originate from naïve or memory cells. One specific example of a type of immunogenic composition is a vaccine.
In some embodiments, an “effective amount” or “immune-stimulatory amount” of an immunogenic composition is an amount which, when administered to a subject, is sufficient to engender a detectable immune response. Such a response may comprise, for instance, generation of an antibody specific to one or more of the epitopes provided in the immunogenic composition. Alternatively, the response may comprise a T-helper or CTL-based response to one or more of the epitopes provided in the immunogenic composition. All three of these responses may originate from naïve or memory cells. In other embodiments, a “protective effective amount” of an immunogenic composition is an amount which, when administered to an animal, is sufficient to confer protective immunity upon the animal.
Inhibiting or treating a disease: Inhibiting the full development of a disease or condition, for example, in a subject who is at risk for a disease. A specific example of diseases is rabies. “Treatment” refers to a therapeutic intervention that ameliorates a sign or symptom of a disease or pathological condition after it has begun to develop. As used herein, the term “ameliorating,” with reference to a disease, pathological condition or symptom, refers to any observable beneficial effect of the treatment. The beneficial effect can be evidenced, for example, by a delayed onset of clinical symptoms of the disease in a susceptible subject, a reduction in severity of some or all clinical symptoms of the disease, a slower progression of the disease, a reduction in the number of relapses of the disease, an improvement in the overall health or well-being of the subject, or by other parameters well known in the art that are specific to the particular disease.
Isolated: An “isolated” or “purified” biological component (such as a nucleic acid, peptide, protein, protein complex, or particle) has been substantially separated, produced apart from, or purified away from other biological components in the cell of the organism in which the component naturally occurs, that is, other chromosomal and extra-chromosomal DNA and RNA, and proteins. Nucleic acids, peptides and proteins that have been “isolated” or “purified” thus include nucleic acids and proteins purified by standard purification methods. The term also embraces nucleic acids, peptides and proteins prepared by recombinant expression in a host cell, as well as chemically synthesized nucleic acids or proteins. The term “isolated” or “purified” does not require absolute purity; rather, it is intended as a relative term. Thus, for example, an isolated biological component is one in which the biological component is more enriched than the biological component is in its natural environment within a cell, or other production vessel. Preferably, a preparation is purified such that the biological component represents at least 50%, such as at least 70%, at least 90%, at least 95%, or greater, of the total biological component content of the preparation.
Label: A detectable compound or composition that is conjugated directly or indirectly to another molecule to facilitate detection of that molecule. Specific, non-limiting examples of labels include fluorescent tags, enzymatic linkages, and radioactive isotopes.
Nucleic acid molecule: A polymeric form of nucleotides, which may include both sense and anti-sense strands of RNA, cDNA, genomic DNA, and synthetic forms and mixed polymers of the above. A nucleotide refers to a ribonucleotide, deoxynucleotide or a modified form of either type of nucleotide. The term “nucleic acid molecule” as used herein is synonymous with “nucleic acid” and “polynucleotide.” A nucleic acid molecule is usually at least 10 bases in length, unless otherwise specified. The term includes single- and double-stranded forms of DNA. A polynucleotide may include either or both naturally occurring and modified nucleotides linked together by naturally occurring and/or non-naturally occurring nucleotide linkages.
Open reading frame (ORF): A series of nucleotide triplets (codons) coding for amino acids without any internal termination codons. These sequences are usually translatable into a peptide/polypeptide/protein/polyprotein.
Operably linked: A first nucleic acid sequence is operably linked with a second nucleic acid sequence when the first nucleic acid sequence is placed in a functional relationship with the second nucleic acid sequence. For instance, a promoter is operably linked to a coding sequence is the promoter affects the transcription or expression of the coding sequence. Generally, operably linked DNA sequences are contiguous and, where necessary to join two protein coding regions, in the same reading frame. If introns are present, the operably linked DNA sequences may not be contiguous.
Pharmaceutically acceptable carriers: The pharmaceutically acceptable carriers useful in this disclosure are conventional. Remington's Pharmaceutical Sciences, by E. W. Martin, Mack Publishing Co., Easton, Pa., 15th Edition (1975), describes compositions and formulations suitable for pharmaceutical delivery of one or more therapeutic compounds or molecules, proteins or antibodies that bind these proteins, and additional pharmaceutical agents.
In general, the nature of the carrier will depend on the particular mode of administration being employed. For instance, parenteral formulations usually comprise injectable fluids that include pharmaceutically and physiologically acceptable fluids such as water, physiological saline, balanced salt solutions, aqueous dextrose, glycerol or the like as a vehicle. For solid compositions (for example, powder, pill, tablet, or capsule forms), conventional non-toxic solid carriers can include, for example, pharmaceutical grades of mannitol, lactose, starch, or magnesium stearate. In addition to biologically-neutral carriers, pharmaceutical compositions to be administered can contain minor amounts of non-toxic auxiliary substances, such as wetting or emulsifying agents, preservatives, and pH buffering agents and the like, for example sodium acetate or sorbitan monolaurate.
Plasmid: A circular nucleic acid molecule capable of autonomous replication in a host cell.
Polypeptide: A polymer in which the monomers are amino acid residues joined together through amide bonds. When the amino acids are alpha-amino acids, either the L-optical isomer or the D-optical isomer can be used, the L-isomers being preferred for many biological uses. The terms “polypeptide” or “protein” as used herein are intended to encompass any amino acid molecule and include modified amino acid molecules. The term “polypeptide” is specifically intended to cover naturally occurring proteins, as well as those which are recombinantly or synthetically produced.
Conservative amino acid substitutions are those substitutions that, when made, least interfere with the properties of the original protein, that is, the structure and especially the function of the protein is conserved and not significantly changed by such substitutions. Examples of conservative substitutions are shown below.














Original 
Conservative 



Residue
Substitutions









Ala
Ser



Arg
Lys



Asn
Gln, His



Asp
Glu



Cys
Ser



Gln
Asn



Glu
Asp



His
Asn; Gln



Ile
Leu, Val



Leu
Ile; Val



Lys
Arg; Gln; Glu



Met
Leu; Ile



Phe
Met; Leu; Tyr



Ser
Thr



Thr
Ser



Trp
Tyr



Tyr
Trp; Phe



Val
Ile; Leu










Conservative substitutions generally maintain (a) the structure of the polypeptide backbone in the area of the substitution, for example, as a sheet or helical conformation, (b) the charge or hydrophobicity of the molecule at the target site, or (c) the bulk of the side chain.
Amino acids are typically classified in one or more categories, including polar, hydrophobic, acidic, basic and aromatic, according to their side chains. Examples of polar amino acids include those having side chain functional groups such as hydroxyl, sulfhydryl, and amide, as well as the acidic and basic amino acids. Polar amino acids include, without limitation, asparagine, cysteine, glutamine, histidine, selenocysteine, serine, threonine, tryptophan and tyrosine. Examples of hydrophobic or non-polar amino acids include those residues having nonpolar aliphatic side chains, such as, without limitation, leucine, isoleucine, valine, glycine, alanine, proline, methionine and phenylalanine Examples of basic amino acid residues include those having a basic side chain, such as an amino or guanidino group. Basic amino acid residues include, without limitation, arginine, homolysine and lysine. Examples of acidic amino acid residues include those having an acidic side chain functional group, such as a carboxy group. Acidic amino acid residues include, without limitation aspartic acid and glutamic acid. Aromatic amino acids include those having an aromatic side chain group. Examples of aromatic amino acids include, without limitation, biphenylalanine, histidine, 2-napthylalananine, pentafluorophenylalanine, phenylalanine, tryptophan and tyrosine. It is noted that some amino acids are classified in more than one group, for example, histidine, tryptophan, and tyrosine are classified as both polar and aromatic amino acids. Additional amino acids that are classified in each of the above groups are known to those of ordinary skill in the art.
Substitutions which in general are expected to produce the greatest changes in protein properties will be non-conservative, for instance changes in which (a) a hydrophilic residue, for example, seryl or threonyl, is substituted for (or by) a hydrophobic residue, for example, leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain, for example, lysyl, arginyl, or histadyl, is substituted for (or by) an electronegative residue, for example, glutamyl or aspartyl; or (d) a residue having a bulky side chain, for example, phenylalanine, is substituted for (or by) one not having a side chain, for example, glycine.
Probes and primers: A probe comprises an isolated nucleic acid molecule attached to a detectable label or other reporter molecule. Typical labels include radioactive isotopes, enzyme substrates, co-factors, ligands, chemiluminescent or fluorescent agents, haptens, and enzymes. Methods for labeling and guidance in the choice of labels appropriate for various purposes are discussed, for example, in Sambrook et al. (ed.), Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989 and Ausubel et al. Short Protocols in Molecular Biology, 4th ed., John Wiley & Sons, Inc., 1999.
Primers are short nucleic acid molecules, for instance DNA oligonucleotides 6 nucleotides or more in length, for example that hybridize to contiguous complementary nucleotides or a sequence to be amplified. Longer DNA oligonucleotides may be about 10, 12, 15, 20, 25, 30, or 50 nucleotides or more in length. Primers can be annealed to a complementary target DNA strand by nucleic acid hybridization to form a hybrid between the primer and the target DNA strand, and then the primer extended along the target DNA strand by a DNA polymerase enzyme. Primer pairs can be used for amplification of a nucleic acid sequence, for example, by the polymerase chain reaction (PCR) or other nucleic-acid amplification methods known in the art. Other examples of amplification include strand displacement amplification, as disclosed in U.S. Pat. No. 5,744,311; transcription-free isothermal amplification, as disclosed in U.S. Pat. No. 6,033,881; repair chain reaction amplification, as disclosed in WO 90/01069; ligase chain reaction amplification, as disclosed in EP-A-320 308; gap filling ligase chain reaction amplification, as disclosed in U.S. Pat. No. 5,427,930; and NASBATM RNA transcription-free amplification, as disclosed in U.S. Pat. No. 6,025,134.
Methods for preparing and using nucleic acid probes and primers are described, for example, in Sambrook et al. (ed.), Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989; Ausubel et al. Short Protocols in Molecular Biology, 4th ed., John Wiley & Sons, Inc., 1999; and Innis et al. PCR Protocols, A Guide to Methods and Applications, Academic Press, Inc., San Diego, Calif., 1990. Amplification primer pairs can be derived from a known sequence, for example, by using computer programs intended for that purpose such as Primer (Version 0.5, © 1991, Whitehead Institute for Biomedical Research, Cambridge, Mass.). One of ordinary skill in the art will appreciate that the specificity of a particular probe or primer increases with its length. Thus, in order to obtain greater specificity, probes and primers can be selected that comprise at least 20, 25, 30, 35, 40, 45, 50 or more consecutive nucleotides of a target nucleotide sequences.
Protein: A biological molecule, particularly a polypeptide, expressed by a gene and comprised of amino acids.
Purified: The term “purified” does not require absolute purity; rather, it is intended as a relative term. Thus, for example, a purified protein preparation is one in which the subject protein is more pure than in its natural environment within a cell. Generally, a protein preparation is purified such that the protein represents at least 50% of the total protein content of the preparation.
Rabies virus (RV): A member of the Rhabdoviridae family having a non-segmented RNA genome with negative sense polarity. Rabies virus is the prototype of the Lyssavirus genus. The rabies virus Evelyn-Rokitnicki-Abelseth (ERA) strain is a strain derived from the Street-Alabama-Dufferin (SAD) strain, first isolated from a rabid dog in Alabama (USA) in 1935. The ERA strain was derived after multiple passages of SAD RV in mouse brains, baby hamster kidney (BHK) cells, and chicken embryos. The complete genomic sequence of the ERA strain is disclosed in PCT Publication No. WO 2007/047459, and the sequence of the ERA strain recovered by reverse genetics is set forth herein as SEQ ID NO: 1.
Recombinant: A recombinant nucleic acid, protein or virus is one that has a sequence that is not naturally occurring or has a sequence that is made by an artificial combination of two otherwise separated segments of sequence. This artificial combination is often accomplished by chemical synthesis or, more commonly, by the artificial manipulation of isolated segments of nucleic acids, for example, by genetic engineering techniques. In some embodiments, recombinant rabies virus is generated using reverse genetics, such as the reverse genetics system described in PCT Publication No. WO 2007/047459. In some examples, the recombinant rabies viruses comprise one or more mutations in a viral virulence factors, such as glycoprotein. In other examples, the recombinant rabies viruses comprise a heterologous gene, such as a sequence encoding an immunocontraceptive peptide (for example, ZP3 or GnRH).
Reverse genetics: Refers to the process of introducing mutations (such as deletions, insertions or point mutations) into the genome of an organism or virus in order to determine the phenotypic effect of the mutation. For example, introduction of a mutation in a specific viral gene enables one to determine the function of the gene.
Sequence identity: The similarity between two nucleic acid sequences, or two amino acid sequences, is expressed in terms of the similarity between the sequences, otherwise referred to as sequence identity. Sequence identity is frequently measured in terms of percentage identity (or similarity or homology); the higher the percentage, the more similar the two sequences are.
Methods of alignment of sequences for comparison are well known in the art. Various programs and alignment algorithms are described in: Smith and Waterman (Adv. Appl. Math., 2:482, 1981); Needleman and Wunsch (J. Mol. Biol., 48:443, 1970); Pearson and Lipman (Proc. Natl. Acad. Sci., 85:2444, 1988); Higgins and Sharp (Gene, 73:237-44, 1988); Higgins and Sharp (CABIOS, 5:151-53, 1989); Corpet et al. (Nuc. Acids Res., 16:10881-90, 1988); Huang et al. (Comp. Appls. Biosci., 8:155-65, 1992); and Pearson et al. (Meth. Mol. Biol., 24:307-31, 1994). Altschul et al. (Nature Genet., 6:119-29, 1994) presents a detailed consideration of sequence alignment methods and homology calculations.
The alignment tools ALIGN (Myers and Miller, CABIOS 4:11-17, 1989) or LFASTA (Pearson and Lipman, 1988) may be used to perform sequence comparisons (Internet Program© 1996, W. R. Pearson and the University of Virginia, “fasta20u63” version 2.0u63, release date December 1996). ALIGN compares entire sequences against one another, while LFASTA compares regions of local similarity. These alignment tools and their respective tutorials are available on the Internet at the NCSA website. Alternatively, for comparisons of amino acid sequences of greater than about 30 amino acids, the “Blast 2 sequences” function can be employed using the default BLOSUM62 matrix set to default parameters, (gap existence cost of 11, and a per residue gap cost of 1). When aligning short peptides (fewer than around 30 amino acids), the alignment should be performed using the “Blast 2 sequences” function, employing the PAM30 matrix set to default parameters (open gap 9, extension gap 1 penalties). The BLAST sequence comparison system is available, for instance, from the NCBI web site; see also Altschul et al., J. Mol. Biol., 215:403-10, 1990; Gish and States, Nature Genet., 3:266-72, 1993; Madden et al., Meth. Enzymol., 266:131-41, 1996; Altschul et al., Nucleic Acids Res., 25:3389-402, 1997; and Zhang and Madden, Genome Res., 7:649-56, 1997.
Orthologs (equivalent to proteins of other species) of proteins are in some instances characterized by possession of greater than 75% sequence identity counted over the full-length alignment with the amino acid sequence of specific protein using ALIGN set to default parameters. Proteins with even greater similarity to a reference sequence will show increasing percentage identities when assessed by this method, such as at least 80%, at least 85%, at least 90%, at least 92%, at least 95%, or at least 98% sequence identity. In addition, sequence identity can be compared over the full length of one or both binding domains of the disclosed fusion proteins.
When significantly less than the entire sequence is being compared for sequence identity, homologous sequences will typically possess at least 80% sequence identity over short windows of 10-20, and may possess sequence identities of at least 85%, at least 90%, at least 95%, or at least 99% depending on their similarity to the reference sequence. Sequence identity over such short windows can be determined using LFASTA; methods are described at the NCSA website. One of skill in the art will appreciate that these sequence identity ranges are provided for guidance only; it is entirely possible that strongly significant homologs could be obtained that fall outside of the ranges provided. Similar homology concepts apply for nucleic acids as are described for protein. An alternative indication that two nucleic acid molecules are closely related is that the two molecules hybridize to each other under stringent conditions.
Nucleic acid sequences that do not show a high degree of identity may nevertheless encode similar amino acid sequences, due to the degeneracy of the genetic code. It is understood that changes in nucleic acid sequence can be made using this degeneracy to produce multiple nucleic acid sequences that each encode substantially the same protein.
Therapeutically effective amount: A quantity of a specified agent sufficient to achieve a desired effect in a subject being treated with that agent. For example, this may be the amount of a recombinant rabies virus useful for eliciting an immune response in a subject and/or for preventing infection by rabies virus. Ideally, in the context of the present disclosure, a therapeutically effective amount of a recombinant rabies virus is an amount sufficient to increase resistance to, prevent, ameliorate, and/or treat infection caused by rabies virus in a subject without causing a substantial cytotoxic effect in the subject. The effective amount of a recombinant rabies virus useful for increasing resistance to, preventing, ameliorating, and/or treating infection in a subject will be dependent on, for example, the subject being treated, the manner of administration of the therapeutic composition and other factors. In some embodiments, the recombinant rabies viruses described herein comprise a nucleic acid sequence encoding an immunocontraceptive protein. For these compositions, a therapeutically effective amount may also refer to the amount of the recombinant rabies virus needed to inhibit fertility, such as preventing or reducing the rate of pregnancy in female animals.
Vector: A nucleic acid molecule as introduced into a host cell, thereby producing a transformed host cell. A vector may include nucleic acid sequences that permit it to replicate in a host cell, such as an origin of replication (DNA sequences that participate in initiating DNA synthesis). A vector may also include one or more selectable marker genes and other genetic elements known in the art.
Virus: Microscopic infectious organism that reproduces inside living cells. A virus typically consists essentially of a core of a single nucleic acid surrounded by a protein coat, and has the ability to replicate only inside a living cell. “Viral replication” is the production of additional virus by the occurrence of at least one viral life cycle. A virus may subvert the host cells' normal functions, causing the cell to behave in a manner determined by the virus. For example, a viral infection may result in a cell producing a cytokine, or responding to a cytokine, when the uninfected cell does not normally do so.
Zona pellucida 3 (ZP3): A glycoprotein expressed on the surface of an egg that serves as the primary receptor for sperm fertilization. ZP3 is also known as zona pellucida glycoprotein 3, zona pellucida protein C (ZPC), sperm receptor and zona pellucida sperm-binding protein 3. As used herein, ZP3 refers to a ZP3 from any animal species, including, but not limited to human, dog, pig, mouse or rat. Exemplary sequences of ZP3 are provided herein, including dog ZP3 (SEQ ID NO: 7 and SEQ ID NO: 62). The term “ZP3” includes ZP3 analogs and variants, including mutated or truncated ZP3.
Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. The singular terms “a,” “an,” and “the” include plural referents unless context clearly indicates otherwise. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. Hence “comprising A or B” means including A, or B, or A and B. It is further to be understood that all base sizes or amino acid sizes, and all molecular weight or molecular mass values, given for nucleic acids or polypeptides are approximate, and are provided for description. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including explanations of terms, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.
IV. Overview of Several Embodiments
It is disclosed herein that recombinant rabies viruses comprising a heterologous sequence encoding an immunocontraceptive peptide can be successfully recovered using a previously described reverse genetics system. In some examples, the immunocontraceptive peptide is GnRH or ZP3. Studies in non-human animals demonstrate that the recombinant rabies viruses described herein elicit high titers of neutralizing antibody specific for rabies virus, induce immunocontraceptive peptide-specific antibodies, protect animals against rabies virus challenge and produce no adverse side effects. It is believed they will provide contraceptive effects in animals to which they are administered.
Provided herein is a recombinant rabies virus, wherein the genome of the recombinant rabies virus comprises a heterologous nucleic acid sequence encoding an immunocontraceptive protein. In some embodiments, the immunocontraceptive protein is gonadotropin-releasing hormone (GnRH) or zona pellucida 3 (ZP3). In some embodiments, the genome of the recombinant rabies virus comprises a nucleic acid sequence encoding GnRH and a nucleic acid sequence encoding ZP3. Generally, the recombinant rabies viruses are generated using a reverse genetics system, such as the system disclosed in PCT Publication No. WO 2007/047459. However, any recombinant rabies viruses comprising a heterologous nucleic acid sequence encoding an immunocontraceptive peptide is contemplated.
In some embodiments, the genome of the recombinant rabies virus is derived from the rabies virus ERA strain. In particular examples, the ERA strain comprises the nucleotide sequence set forth as SEQ ID NO: 1. Although the ERA strain is exemplified herein, any suitable strain of rabies virus can be used. An appropriate rabies virus strain can be selected by one of skill in the art. Examples of rabies virus strains include, but are not limited to CVS, ERA, PV, SAD-B19 and HEP-Flury, SAG1, SAG2 and RC-HL.
In some embodiments, the genome of the recombinant rabies virus is engineered such that the rabies virus gene sequences are rearranged. In some examples, the glycoprotein (G) gene is relocated between the N and P genes, such that the rabies virus genes are in the following order: 3′-N-G-P-M-L-S′ (see FIG. 5A). This type of virus, when derived from the ERA strain, is referred to herein as ERAg3p. Although relocation of the G gene is exemplified herein, any other rearrangements of the rabies virus genes are contemplated, as long as recombinant virus can be recovered using reverse genetics.
In some embodiments, the rabies virus strain is an attenuated strain. In some examples, the glycoprotein of the recombinant rabies virus comprises a Glu at amino acid position 333 (corresponding to residue 352 of SEQ ID NO: 5). Other rabies virus attenuating mutations are known in the art and can be used with the compositions and methods provided herein.
The ZP3 nucleic acid sequence can be a ZP3 sequence from any animal species, such as human, pig, rat, mouse or dog. In some embodiments, the ZP3 nucleic acid sequence is a dog ZP3 nucleic acid sequence. In some examples, the dog ZP3 nucleic acid sequence is SEQ ID NO: 7. In some embodiments, the GnRH nucleic acid sequence is SEQ ID NO: 47. The ZP3 nucleic acid sequence incorporated into the recombinant rabies virus need not be 100% identical to a ZP3 nucleic acid sequence known in the art or disclosed herein. Similarly, the GnRH nucleic acid sequence incorporated into the recombinant rabies virus can be from any animal species, and need not be 100% identical to a GnRH nucleic acid sequence known in the art or disclosed herein. Rather, the ZP3 or GnRH nucleic acid sequence need only be capable of eliciting an immune response in the animal in which the recombinant rabies virus is administered. In some embodiments, the ZP3 nucleic acid sequence is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% or 100% identical to SEQ ID NO: 7. In some embodiments, the GnRH nucleic acid sequence is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% or 100% identical to SEQ ID NO: 47.
In some embodiments, the recombinant rabies viruses comprise a single copy of the ZP3 or GnRH nucleic acid sequence, or a single copy of each sequence. In other embodiments, the recombinant rabies viruses comprise multiple copies of the ZP3 or GnRH nucleic acid sequence (or another immunocontraceptive peptide), such as two, three, four, five, six, seven, eight or nine copies of one or both of the ZP3 and GnRH nucleic acid sequences. When multiple copies of the ZP3 and/or GnRH nucleic acid sequence are used, the copies can be inserted in the genome of the recombinant rabies virus such that the sequences are contiguous. Alternatively, the multiple copies of the ZP3 or GnRH nucleic acid sequences can be inserted at different positions within the rabies virus genome, such as in different genes, or at different sites within the same gene.
In some embodiments, the heterologous sequence encoding the immunocontraceptive peptide is inserted within or adjacent to the rabies virus glycoprotein gene. In particular examples, the heterologous sequence is inserted following the signal sequence of glycoprotein. In other embodiments, the heterologous sequence is inserted at or near (such as immediately following) antigenic site IIa of glycoprotein. In other embodiments, the heterologous sequence is inserted between the ectodomain and transmembrane domain of glycoprotein. In particular examples, the heterologous nucleic acid sequence is inserted following the signal sequence (nucleotides 1-57 of SEQ ID NO: 49) of the glycoprotein gene. In some cases, when the GnRH sequence is inserted at this site, the glycoprotein gene comprises the nucleic acid sequence of SEQ ID NO: 49 (single copy of GnRH) or SEQ ID NO: 51 (two tandem copies of GnRH). In some examples, when the GnRH sequence is inserted at antigenic site IIa (nucleotide 663 of SEQ ID NO: 53) of the glycoprotein gene, the glycoprotein gene comprises the nucleic acid sequence of SEQ ID NO: 53. In some examples, when the GnRH sequence is inserted at the junction of the ectodomain and transmembrane domain of glycoprotein (following nucleotide 1374 of SEQ ID NO: 63), the glycoprotein gene comprises the nucleic acid sequence of SEQ ID NO: 63. In other specific examples, the ZP3 nucleic acid sequence is inserted between the rabies virus P and M genes. In some embodiments, the recombinant rabies virus is a rabies virus listed in FIG. 3A or Table 3.
Also provided herein are immunogenic compositions comprising one or more of the recombinant rabies viruses described herein. Further provided is an immunogenic composition comprising a first recombinant rabies virus and a second recombinant rabies virus, wherein the genome of the first recombinant rabies virus comprises a GnRH nucleic acid sequence and the genome of the second recombinant rabies virus comprises a ZP3 nucleic acid sequence. The first recombinant rabies varies can be any recombinant rabies virus comprising a nucleic acid sequence encoding GnRH, as described herein. The second recombinant rabies virus can be any recombinant rabies virus comprising a nucleic acid sequence encoding ZP3, as described herein. In some embodiments, the immunogenic compositions further comprise a pharmaceutically acceptable carrier. In some embodiments, the immunogenic compositions further comprise an adjuvant.
Also provided is a method of immunizing a non-human animal against rabies virus infection and inhibiting fertility of the animal, comprising administering to the animal a therapeutically effective amount of an immunogenic composition comprising one or more of the recombinant rabies viruses described herein. The composition can be administered using any suitable route. In some embodiments, the immunogenic composition is administered orally, such as through food-baits. The animal can be any animal susceptible to rabies virus infection for which population control is desired. In some embodiments, the animal is a domestic animal. In other embodiments, the animal is a wild animal. In some embodiments, the animal is a dog, cat, rat, mouse, bat, fox, raccoon, squirrel, opossum, coyote or wolf.
Also provided herein is the use of a composition comprising one or more recombinant rabies viruses with a genome encoding one or more immunocontraceptive peptides in the manufacture of a medicament for immunizing a non-human animal against rabies virus infection and inhibiting fertility of the animal. Further provided are compositions comprising one or more recombinant rabies viruses with a genome encoding one or more immunocontraceptive peptides for use in a method of immunizing a non-human animal against rabies virus infection and inhibiting fertility of the animal.
V. Determinants of Rabies Virus Pathogenicity
The rabies virus (RV) is a rhabdovirus—a non-segmented RNA virus with negative sense polarity. Within the Rhabdoviridae family, rabies virus is the prototype of the Lyssavirus genus. RV is composed of two major structural components, a nucleocapsid or ribonucleoprotein (RNP), and an envelope in the form of a bilayer membrane surrounding the RNP core. The infectious component of all rhabdoviruses is the RNP core, which consists of the negative strand RNA genome encapsidated by nucleoprotein (N) in combination with RNA-dependent RNA-polymerase (L) and phosphoprotein (P). The membrane surrounding the RNP contains two proteins, the trans-membrane glycoprotein (G) and the matrix (M) protein, located at the inner site of the membrane. Thus, the viral genome codes for these five proteins: the three proteins in the RNP (N, L and P), the matrix protein (M), and the glycoprotein (G).
The molecular determinants of pathogenicity of various rabies virus strains have not been fully elucidated. RV pathogenicity was attributed to multigenic events (Yamada et al., Microbiol. Immunol. 50:25-32, 2006). For example, some positions in the RV genome if mutated, affect viral transcription or replication, reducing virulence. Mutations at serine residue 389 of the phosphorylation site in the N gene (Wu et al., J. Virol. 76:4153-4161, 2002) or GDN core sequence of the highly conserved C motif in the L gene (Schnell and Conzelmann, Virol. 214:522-530, 1995) dramatically reduced RV transcription and replication.
The G protein, also referred to as spike protein, is involved in cell attachment and membrane fusion of RV. The amino acid region at position 330 to 340 (referred to as antigenic site III) of the G protein has been identified as important for virulence of certain strains of RV. Several studies support the concept that the pathogenicity of fixed RV strains is determined by the presence of arginine or lysine at amino acid residue 333 of the glycoprotein (Dietzschold et al., Proc. Natl. Acad. Sci. USA 80: 70-74, 1983; Tuffereau et al., Virol. 172: 206-212, 1989).
This phenomenon seems to apply at least to fixed rabies viruses such as CVS, ERA, PV, SAD-B19 and HEP-Flury strains (Anilionis et al., Nature 294:275-278, 1981; Morimoto et al., Virol. 173:465-477, 1989). For example, rabies vaccine viruses possessing an amino acid differing from Arg at position 333 of the glycoprotein are described, for instance, in WO 00/32755 (describing RV mutants in which all three nucleotides in the G protein Arg333 codon are altered compared to the parent virus, such that the Arg at position 333 is substituted with another amino acid); European Patent 350398 (describing an avirulent RV mutant SAG1 derived from the Bern SAD strain of RV, in which the Arg at position 333 of the glycoprotein has been substituted to Ser); and European patent application 583998 (describing an attenuated RV mutant, SAG2, in which the Arg at position 333 in the G protein has been substituted by Glu).
Other strains, such as the RC-HL strain, possess an arginine residue at position 333 of the G, but do not cause lethal infection in adult mice (Ito et al., Microl. Immunol. 38:479-482, 1994; Ito et al., J. Virol. 75:9121-9128, 2001). As such, the entire G may contribute to the virulence of RV, although the determinants or regions have not previously been identified.
The G gene encodes the only protein that induces viral neutralizing antibodies. At least three states of RV glycoprotein are known: the native state (N) being responsible for receptor binding; an active hydrophobic state (A) necessary in the initial step in membrane fusion process (Gaudin, J. Cell Biol. 150:601-612, 2000), and a fusion inactive conformation (I). Correct folding and maturation of the G protein play important roles for immune recognition. The three potential glycosylated positions in ERA G extracellular domain occur at Asn37, Asn247 and Asn319 residues (Wojczyk et al., Glycobiology. 8: 121-130, 1998). Nonglycosylation of G not only affects conformation, but also inhibits presentation of the protein at the cell surface.
It has been previously demonstrated (see PCT Publication No. WO 2007/047459) that expression of G enhances the anti-RV immune response. In addition, introduction of an Arg to Glu mutation at amino acid position 333 of RV ERA glycoprotein results in an attenuated virus (referred to as ERAg3). This attenuated virus is capable of eliciting significant titers of neutralizing antibodies in animals and conferring protection against wild-type virus challenge. Furthermore, as described in PCT Publication No. WO 2007/047459, a recombinant RV comprising two copies of glycoprotein with the G333 mutation is particularly useful as a vaccine due to its ability to elicit high titers of neutralizing antibodies without morbidity or mortality. In some examples herein, a recombinant rabies virus comprising the G333 mutation in glycoprotein is used to engineer immunocontraceptive compositions comprising ZP3 and/or GnRH. However, one of ordinary skill in the art will recognize that any one of a number of recombinant rabies viruses can be used to incorporate heterologous sequences using the reverse genetics systems disclosed in PCT Publication No. WO 2007/047459, and as summarized below.
VI. Rabies Virus Reverse Genetics System
RNA cannot readily be manipulated directly by molecular biological methods. Traditional RNA virus vaccines are from naturally attenuated isolates, which are difficult to control and provide unpredictable results. Reverse genetics technology makes it possible to manipulate RNA viruses as DNA, which can be mutated, deleted or reconstructed according to deliberate designs. Every gene function can be studied carefully, independently, and in concert, which benefits vaccine development. Reverse genetics involves reverse transcription of the RNA viral genome into cDNA, and cloning into a vector, such as a plasmid. After transfection of host cells, the vector is transcribed into RNA, to be encapsidated by viral structural proteins, which can also be supplied by plasmids. The encapsidated RNA forms a ribonucleoprotein complex, which results in virions that can be recovered.
An efficient reverse genetics system based on the rabies virus ERA strain is described in PCT Publication No. WO 2007/047459. This rabies reverse genetics system is useful for a variety of purposes, including to attenuate ERA virus in a defined manner for vaccine development and to produce ERA virus vectors for expression of heterologous proteins, such as proteins for immunocontraception, including ZP3 and GnRH.
The reverse genetics system disclosed in PCT Publication No. WO 2007/047459 is based on a full length transcription plasmid plus a plurality of helper plasmids (e.g., five helper plasmids). The helper plasmids encode the N, P, L proteins, and optionally the G protein, as well as the T7 polymerase. Although the G protein is not necessary for virus rescue, it improves virus recovery efficiency or virus budding when included in transfection.
Transcription involves both cellular RNA-dependent RNA polymerase II, which is available in mammalian cells, and T7 RNA polymerase, which is supplied by pNLST7 plasmids. The dual polymerases result in virus recovery efficiency that is both high and stable.
In the transcription plasmid, hammerhead and hepatitis delta virus ribozymes flank a rabies virus (e.g., ERA strain) antigenomic cDNA, enabling the production of authentic 5′ and 3′ ends of antigenomic viral RNA by transcription. The first ten nucleotides of the hammerhead sequence are designed to be complementary to the first ten nucleotides of the antisense genomic sequence.
Two modified T7 RNA polymerase constructs support virus recovery more efficiently than the wild type T7 RNA polymerase applied previously. One T7 RNA polymerase has been mutated from the first ATG to AT. The second T7 RNA polymerase has an eight amino acid nuclear localization signal (NLS) derived from the SV40 virus large T antigen fused after the first ATG from the parental T7. Addition of the NLS results in the T7 RNA polymerase being present predominantly in the nucleus. Following transfection mechanism of the NLS modified plasmid, the DNA/transfection reagent complex binds to the surface of the cell. Through endocytosis, the complex is taken into the endosome/lysosome, and the DNA is released into the cytosol. In the absence of the NLS, the majority of the transfected plasmids are retained in the cytosol and only a small percentage of the released DNA reaches the nucleus, where it is transcribed into RNA. After protein synthesis, the NLST7 RNA polymerase is transported back to the cell nucleus, and the helper plasmids (with T7/CMV promoters) in the nucleus will be transcribed by both NLST7 and cellular polymerase II. Thus, more mRNAs of the helper plasmids and cRNA of the full-length pTMF or its derivatives are synthesized and result in high efficiency of virus recovery.
After the initial expression of NLST7 by CMV promoter, NLST7 polymerase binds to pT7 for transcription of NLST7 gene. Through modification of the transcripts in the nucleus, more NLST7 mRNA is synthesized, resulting in more expression of NLST7 polymerase. The pT7 of the NLST7 polymerase as well as of the full length antigenomic transcription unit is under the control of the NLST7 polymerase, which acts as an “autogene.” After expression of T7 RNA polymerase in the nucleus, the transfected T7 constructs continue to transcribe full length RNA template for N protein encapsidation and/or L protein binding, enhancing virus recovery efficiency.
The T7 polymerase, and all other plasmids, except the N protein encoding plasmid pTN, are placed under control of both CMV and T7 transcriptional regulatory elements. The N protein encoding nucleic acid is under the control of a T7 promoter and is translated in cap-independent manner based on an IRES (internal ribosome entry site). Cellular RNA polymerase II alone can help the recovery of RV if all the plasmids were cloned under the control of the CMV promoter. In the ERA reverse genetics system disclosed in PCT Publication No. WO 2007/047459, only pTN is under the control of the T7 promoter and is translated in a cap-independent manner. All other constructs are under control of both CMV and the T7 transcriptional regulatory elements. Typically, in RV, N synthesis is abundant and the ratio among N, P and L is approximately 50:25:1. To mimic the wild type viral transcription and assembly in RV reverse genetics, N expression should be the highest. With the aid of NLST7 polymerase and IRES translation mode, N protein is expressed efficiently after plasmid transfection. This reduces competition for transcription with house keeping genes in host cells, because the T7 transcription initiation signal does not exist in mammalian cells, and results in increased efficiency of T7 transcription.
In addition, as described in PCT Publication No. WO 2007/047459, to enhance production of viral proteins, the helper plasmids can be constructed to incorporate a Kozak sequence that has been optimized for the translation efficiency for each protein encoding sequence. After five days post-transfection in the ERA reverse genetics system, the rescued viruses reliably and repeatably grew to 107 FFU/ml without further amplification.
Recombinant rabies viruses with favorable properties for vaccination can be designed using, for example, the reverse genetics system disclosed in PCT Publication No. WO 2007/047459. Modified strains having mutated glycoproteins are particularly suited for use as immunogenic compositions. This RV reverse genetics system also enables a rabies virus vector system for foreign (heterologous) gene expression. An extra transcription unit was demonstrated to be functional in two different locations after incorporation into the ERA RV genome. Thus, the RV reverse genetics system provides a means for introducing heterologous proteins that serve as immunocontraceptives. In some examples, the heterologous protein is ZP3, GnRH, or both.
VII. Immunocontraception
Provided herein are recombinant rabies viruses comprising within their genome heterologous nucleic acid sequences encoding one or more immunocontraceptive proteins. An immunocontraceptive protein refers to any protein or protein fragment (also referred to as an “antigen”) capable of eliciting an immune response in a subject that results in inhibition or loss of fertility in the subject to which the antigen is administered. The recombinant rabies viruses described herein are contemplated for vaccination of non-human animals.
Immunocontraception involves vaccination against sperm, eggs or reproductive hormones to prevent fertilization or the production of gametes (Cooper and Larsen, Reproduction 132:821-828, 2006). Immunogens previously tested as immunocontraceptives include sperm antigens, whole sperm, lactate dehydrogenase (LDH-C4; a sperm-specific protein), fertilization antigen-1 (FA-1; a sperm-specific antigen), sperm protein 56 (sp56), eppin (a testis/epididymis protein), oocyte antigens (such as zona pellucida), gonadotropin riboflavin carrier protein, prolactin, proliferin, gonadotropins and gonadotropin releasing hormones (Delves et al., Trends Immunol. 23:213-219, 2002; O'Hern et al., Vaccine 15(16):1761-1766, 1997; Zhu and Naz, Proc. Natl. Acad. Sci. 94(9):4704-4709, 1997; Hardy and Mobbs, Mol. Reprod. Dev. 52(2):216-224, 1999; Hardy et al., Reproduction Supplement 60:19-30, 2002; O'Rand et al., Science 306:1189-1190, 2004; Cooper and Larsen, Reproduction 132:821-828, 2006).
A number of immunocontraceptive studies have focused on the use of either zona pellucida (ZP) or GnRH. However, in every case, it was necessary to administer an adjuvant with the ZP or GnRH proteins in order to elicit a sufficient immune response to inhibit fertility of the treated animals. It is disclosed herein that recombinant rabies viruses comprising ZP and/or GnRH can be used as immunocontraceptive compositions. The super-antigen like features of the rabies virus particle allow for the use of recombinant rabies viruses comprising an immunocontraceptive protein in the absence of an adjuvant.
Gonadotropin-Release Hormone (GnRH)
GnRH (also known as luteinizing hormone releasing hormone, or LHRH) has long been recognized as playing a central role in the regulation of fertility in animals. The fully processed form of GnRH is a decapeptide which has the same amino acid sequence in all mammals (SEQ ID NO: 48). Closely related GnRH compounds have also been identified in other non-mammals, including fowl, and receptors for GnRH have been identified in reptiles and amphibians. In males and females, GnRH is released from the hypothalamus into the bloodstream and travels via the blood to the pituitary, where it induces the release of the gonadotropins, luteinizing hormone (LH) and follicle stimulating hormone (FSH). These two gonadotropins in turn act upon the gonads, inducing steroidogenesis and gametogenesis. In growing male animals, the gonadotropins stimulate the development of the testes and the synthesis of testicular steroids. In the growing female animal, the development of the ovaries is stimulated and therein follicle development, synthesis of ovarian steroids and ovulation. Steroids released from the gonads into the circulation also act upon various other tissues (U.S. Patent Publication No. 2006/0013821).
A variety of GnRH immunogenic analogs have also been described and are suitable for use with the compositions and methods provided herein. Immunogenic analogs of GnRH include compounds containing a substitution, deletion, or insertion of between one and five amino acid residues in the GnRH amino acid sequence, as well as dimers or polymers thereof, which compound retains the ability to induce or stimulate the production in an animal of antibodies specific for GnRH. The substitutions and insertions can be accomplished with natural or non-natural amino acids, and substitutions are preferably conservative substitutions made with amino acids which maintain substantially the same charge and hydrophobicity as the original amino acid. Immunogenic analogs of GnRH include those described in, for example, U.S. Pat. Nos. 5,484,592; 6,284,733; 4,608,251; 5,759,551; and 5,403,586, and PCT Publication No. WO 88/05308.
Zona Pellucida (ZP)
ZP is a non-cellular glycoprotein coat surrounding mammalian eggs which regulates sperm-egg interactions during fertilization. The structure of ZP makes it an ideal candidate for a contraceptive target, since altering its structure can prevent pregnancy (U.S. Patent Publication No. 2004/0202674).
ZP immunization has been effective in lowering fertilization rates of many mammals (Willis et al., J. Equine Vet. Sci. 14:364-370, 1994; Brown et al., J. Reprod. Immunol. 35:43-51, 1997; Brown et al., J. Reprod. Immunol. 35:53-64, 1997; U.S. Pat. No. 6,027,727). Two independent reports indicated that pig zona pellucida (pZP) is an effective immunocontraceptive in domestic cats, however multiple boosters are required (Ivanova et al., Theriogenology 43:969-981, 1995; Bradley et al., J. Biotechnol. 73:91-101, 1999).
Porcine zona pellucida has also been used in liposome-based immunocontraceptive vaccines for reducing fertility of certain mammals by 90-100% with a multi-year efficacy (PCT Publication NO. WO 93/25231). However, use of pZP in such a liposome-based vaccine as a single administration vaccine is ineffective in cats (Gorman et al., Theriogenology 58:135-149, 2002).
ZP3 sequences from a variety of different species are well known in the art, including dog ZP3 (Genbank Accession No. NM—001003224, deposited on Aug. 5, 2004); porcine ZP3 (Genbank Accession No. D45065, deposited on Jan. 24, 1995; Genbank Accession No. NM 213893, deposited on May 20, 2004); mouse ZP3 (Genbank Accession No. BC103585, deposited on Aug. 22, 2005; Genbank Accession No. BC099465, deposited on Jul. 21, 2005; Genbank Accession No. BC103584, deposited on Aug. 22, 2005); rat ZP3 (Genbank Accession No. BC127488, deposited on Dec. 22, 2006); and human ZP3 (Genbank Accession No. BC113949, deposited on Feb. 25, 2006; Genbank Accession No. X56777, deposited on Jun. 16, 1993; Genbank Accession No. M60504, deposited on Aug. 4, 1993; Genbank Accession No. A18567, deposited on Jul. 21, 1994). Each of the above-listed Genbank Accession numbers is herein incorporated by reference. In specific examples herein, the ZP3 sequence is a dog ZP3 sequence (SEQ ID NO: 7). However, any ZP3 sequence capable of eliciting an immune response in the animal to be vaccinated can be used with the compositions and methods provided herein.
VIII. Administration and Use of Rabies Virus Immunocontraceptive Compositions
The recombinant rabies viruses provided herein comprise at least one heterologous nucleic acid sequence encoding an immunocontraceptive protein. Thus, immunocontraceptive compositions comprising such recombinant rabies viruses have a dual function: (i) to protect vaccinated animals against rabies virus infection and (ii) to control animal population growth by inhibiting fertility of the animals. Accordingly, the immunocontraceptive compositions provided herein are contemplated for use with non-human animals. In some cases, the recombinant rabies virus is administered to domestic animals. In other cases, the recombinant rabies virus is administered to wild animals. Non-human animals for which the rabies virus immunocontraceptive compositions will be useful may include, but is not limited to, dogs, cats, rats, mice, bats, foxes, raccoons, squirrels, opossum, coyotes or wolves. Particularly with wild animals, it is preferred to administer the immunogenic composition orally, such as through food-baits.
The immunogenic formulations may be conveniently presented in unit dosage form and prepared using conventional pharmaceutical techniques. Such techniques include the step of bringing into association the active ingredient and the pharmaceutical carrier(s) or excipient(s). In general, the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers. Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example, sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of a sterile liquid carrier, for example, water for injections, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets commonly used by one of ordinary skill in the art.
In certain embodiments, unit dosage formulations are those containing a dose or unit, or an appropriate fraction thereof, of the administered ingredient. It should be understood that in addition to the ingredients particularly mentioned above, formulations encompassed herein may include other agents commonly used by one of ordinary skill in the art.
The compositions provided herein, including those for use as immunogenic compositions, may be administered through different routes, such as oral, including buccal and sublingual, rectal, parenteral, aerosol, nasal, intramuscular, subcutaneous, intradermal, and topical. They may be administered in different forms, including but not limited to solutions, emulsions and suspensions, microspheres, particles, microparticles, nanoparticles, and liposomes. In preferred embodiments, the immunogenic compositions are administered orally. In some examples, oral administration comprises administering the compositions in food-baits.
The volume of administration will vary depending on the route of administration. Those of ordinary skill in the art will know appropriate volumes for different routes of administration.
Administration can be accomplished by single or multiple doses. The dose administered to an animal in the context of the present disclosure should be sufficient to induce a beneficial therapeutic response over time, such as to prevent RV infection and prevent reproduction. The dose required will vary depending on, for example, the species of animal.
The amount of immunogenic composition in each dose is selected as an amount that induces an immunostimulatory or immunoprotective response without significant, adverse side effects. Such amount will vary depending upon which specific composition is employed and how it is administered. Initial doses may range from about 1 μg to about 1 mg, with some embodiments having a range of about 10 μg to about 800 μg, and still other embodiments a range of from about 25 μg to about 500 μg. Following an initial administration of the immunogenic composition, subjects may receive one or several booster administrations, adequately spaced. Booster administrations may range from about 1 μg to about 1 mg, with other embodiments having a range of about 10 μg to about 750 μg, and still others a range of about 50 μg to about 500 μg. Periodic boosters at intervals of 1-5 years, for instance three years, may be desirable to maintain the desired levels of protective immunity. In preferred embodiments, animals receive a single dose of an immunogenic composition.
The preparation of food-baits containing immunogenic compositions is also within the ordinary skill of those in the art. For example, the preparation of food-baits containing live RV vaccines is disclosed in Wandeler et al. (Rev. Infect. Dis. 10 (suppl. 4):649-653, 1988), Aubert et al. (pp. 219-243, in Lyssaviruses (Rupprecht et al., eds.), Springer-Verlag, New York, 1994), and Fu et al. (pp. 607-617, in New Generation Vaccines (2nd Edit.) (Levine et al., eds.), Marcel Dekker, Inc., New York, 1997).
Provided herein are pharmaceutical compositions (also referred to as immunogenic or immunostimulatory compositions) which include a therapeutically effective amount of a recombinant RV alone or in combination with a pharmaceutically acceptable carrier. In some embodiments, the recombinant RV comprises a heterologous protein, such as ZP3 and/or GnRH.
Pharmaceutically acceptable carriers include, but are not limited to, saline, buffered saline, dextrose, water, glycerol, ethanol, and combinations thereof. The carrier and composition can be sterile, and the formulation suits the mode of administration. The composition can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents. The composition can be a liquid solution, suspension, emulsion, tablet, pill, capsule, sustained release formulation, or powder. The composition can be formulated as a suppository, with traditional binders and carriers such as triglycerides. Oral formulations can include standard carriers such as pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharine, cellulose, and magnesium carbonate. Any of the common pharmaceutical carriers, such as sterile saline solution or sesame oil, can be used. The medium can also contain conventional pharmaceutical adjunct materials such as, for example, pharmaceutically acceptable salts to adjust the osmotic pressure, buffers, preservatives and the like. Other media that can be used with the compositions and methods provided herein are normal saline and sesame oil.
The recombinant RVs described herein can be administered alone or in combination with other therapeutic agents to enhance antigenicity. For example, the recombinant viruses can be administered with an adjuvant, such as Freund incomplete adjuvant or Freund's complete adjuvant.
Optionally, one or more cytokines, such as IL-2, IL-6, IL-12, RANTES, GM-CSF, TNF-α, or IFN-γ, one or more growth factors, such as GM-CSF or G-CSF; one or more molecules such as OX-40L or 41 BBL, or combinations of these molecules, can be used as biological adjuvants (see, for example, Salgaller et al., 1998, J. Surg. Oncol. 68(2):122-38; Lotze et al., 2000, Cancer J. Sci. Am. 6(Suppl 1):S61-6; Cao et al., 1998, Stem Cells 16(Suppl 1):251-60; Kuiper et al., 2000, Adv. Exp. Med. Biol. 465:381-90). These molecules can be administered systemically (or locally) to the host.
A number of means for inducing cellular responses, both in vitro and in vivo, are known. Lipids have been identified as agents capable of assisting in priming CTL in vivo against various antigens. For example, as described in U.S. Pat. No. 5,662,907, palmitic acid residues can be attached to the alpha and epsilon amino groups of a lysine residue and then linked (for example, via one or more linking residues, such as glycine, glycine-glycine, serine, serine-serine, or the like) to an immunogenic peptide. The lipidated peptide can then be injected directly in a micellar form, incorporated in a liposome, or emulsified in an adjuvant. As another example, E. coli lipoproteins, such as tripalmitoyl-S-glycerylcysteinlyseryl-serine can be used to prime tumor specific CTL when covalently attached to an appropriate peptide (see, Deres et al., Nature 342:561, 1989). Further, as the induction of neutralizing antibodies can also be primed with the same molecule conjugated to a peptide which displays an appropriate epitope, two compositions can be combined to elicit both humoral and cell-mediated responses where that is deemed desirable.
The following examples are provided to illustrate certain particular features and/or embodiments. These examples should not be construed to limit the disclosure to the particular features or embodiments described.
EXAMPLES
Example 1
Rabies Virus ERA-Based Immunocontraceptive Studies Using Dog ZP3
This example describes the development of an immunocontraceptive composition comprising a recombinant rabies virus ERA strain and dog zona pellucida 3 (ZP3). Immunocontraceptive studies based on porcine zona pellucida (pZP) glycoprotein have been attempted in different animals, including dogs. The pZP complex was reported to be effective in a number of species as an immunocontraceptive. However, because the pZP complex is a mixture of whole porcine ovary, adverse reactions are not uncommon. Therefore, a canine ZP3 glycoprotein was expressed in E. coli and a dog ZP3 gene was cloned as a DNA vaccine candidate. The rationale was to develop a rabies virus ERA-based immunocontraceptive vaccine that can control rabies virus and dog population simultaneously. Rabies virus ERA has proved to be an ideal vector for expression of heterologous genes. Furthermore, it has been demonstrated that modified ERA virus is effective as an oral vaccine candidate in various animal species (see PCT Publication No. WO 2007/047459).
Full length dog ZP3 was synthesized chemically and assembled by polymerase chain reaction (PCR). Dog ZP3 is 1278 base pairs in length and encodes a protein of 426 amino acids. The synthesized gene is set forth herein as SEQ ID NO: 7; the amino acid sequence is set forth as SEQ ID NO: 8. To synthesize the dog ZP3 gene, the full length dog ZP3 gene was divided into two fragments for synthesis, which are referred to as the A and B fragments. Fragment A (619 base pairs), which starts from the ATG start codon and ends with the unique NdeI recognition site, was assembled with 18 oligonucleotides (Table 1). Fragment B (670 base pairs) starts from unique NdeI recognition site and continues to the stop codon (TAA) and was assembled by 20 oligonucleotides (Table 1). The method for designing the oligonucleotides was based on “inside-out gene synthesis” using the DNAWorks program (Hoover and Lubkowski, Nucleic Acids Res. 30(10):e43, 2002).
After the A and B fragments were successfully synthesized, they were sequenced carefully to correct any potential mutations introduced during the PCR reactions. One silent mutation (which does not change the amino acids sequence) from C to T was purposely maintained to distinguish the synthesized gene from the template gene (Genbank Accession Number NM—001003224, deposited on Aug. 5, 2004, SEQ ID NO: 62). The oligonucleotides for synthesis of the A and B fragments are shown in Table 1.







TABLE 1







Oligonucleotides for synthesis of dog ZP3









OLIGO
SEQUENCE
SEQ ID NO:












 1A
AAAACTGCAGCCACCATG
9


 


 2A
AACTGCAGCCACCATGGGGCTGAGCTATGGA
10



ATTTTCATCTGTTTTCTGCTCCT



 


 3A
TTTCATCTGTTTTCTGCTCCTGGGAGGCATGG
11



AGCTGTGCTGCCCCCAGACCAT



 


 4A
CTGCCCCCAGACCATCTGGCCAACTGAGACC
12



TACTACCCATTGACATCTAGGCC



 


 5A
CCCATTGACATCTAGGCCCCCAGTAATGGTG
13



GACTGTCTGGAGTCCCAGCTGGT



 


 6A
GGAGTCCCAGCTGGTGGTCACTGTCAGCAAA
14



GACCTTTTTGGTACTGGGAAGCT



 


 7A
CTTTTTGGTTACGGGAAGCTCATCAGGCCAG
15



CAGACCTCACCCTGGGTCCAGAG



 


 8A
CACCCTGGGTCCAGAGAACTGTGAGCCCCTG
16



GTCTCCATGGACACGGATGATGT



 


 9A
CATGGACACGGATGATGTGGTCAGGTTTGAG
17



GTTGGGCTGCACGAGTGTGGCAG



 


10A
GTGCTGTACACCAGAGCATTGTCAGTCACCT
18



GCACCCTGCTGCCACACTCGTGC



 


11A
CAGGTTGCCCGCAGGGCGGGGGCTGTGGATC
19



AGGAAGGTGCTGTACACCAGAGC



 


12A
ACTCGATGGGGACCTCGGCACGATTAGTTCT
20



CAGGATGGACAGGTTGCCCGCAG



 


13A
GGCCTGGCTGCTCACATTGCTGTGCCTGGGG
21



TAGTGGCACTCGATGGGGACCTC



 


14A
AGAGCATTGTGGTCCTGAAGGGCACCCAAGT
22



GGGCAGGATGGCCTGGCTGCTCA



 


15A
CCATTAGGCGGAGAGAGAAAACTAGCTTCTC
23



CTCGAAGAGCATTGTGGTCCTGA



 


16A
ATGTGGGGGATTGCTTCTCGGAGCCCCAGTC
24



CTCCTCCATTAGGCGGAGAGAGA



 


17A
CTTCAGCCTGGAGGTGGGCTATGTCTCCCAG
25



CTGGAATGTGGGGGATTGCTTCT



 


18A
ACAAAAAGTCGCAGTGGCATATGGCTGCCAG
26



TGTGGACTTCAGCCTGGAGGTG



 


 1B
TGGCAGCCATATGCCACTGCGACTTTTTGTG
27



GACCACTGT



 


 2B
GACTTTTTGTGGACCACTGTGTGGCCACGCT
28



GACACCAGATCGGAATGCCTTCC



 


 3B
CAGATCGGAATGCCTTCCCTCATCACAAAAT
29



TGTGGACTTCCATGGCTGTCTTG



 


 4B
GACTTCCATGGCTGTCTTGTGGATGGTCTCTA 
30



CAATTCCTCTTCAGCCTTCAAA



 


 5B
AATTCCTCTTCAGCCTTCAAAGCCCCCAGAC
31



CCAGGCCAGAGACTCTTCAGTTC



 


 6B
GCCAGAGACTCTTCAGTTCACAGTGGATGTT
32



TTCCACTTTGCTAAGGACTCAAG



 


 7B
CCACTTTGCTAAGGACTCAAGAAACACGATC
33



TATATCACCTGCCATCTGAAGGT



 


 8B
ACCTGCCATCTGAAGGTCACTCCGGCTGACC
34



GAGTCCCAGACCAGCTAAACAAA



 


 9B
CCCAGACCAGCTAAACAAAGCTTGTTCCTTC
35



ATCAAGTCTACCAAGAGGTCCTA



 


10B
CAAGTCTACCAAGAGGTCCTACCCTGTAGAA
36



GGCTCGGCTGATATTTGTCGCTG



 


11B
ACCGGCCTGGAAGGCCACAGCTGCCTTTGTT
37



ACAACAGCGACAAATATCAGCCG



 


12B
GACCTGCGCCACCCTCTCTCTAGGTGGGACA
38



GCCTCCTGGACCGGCCTGGAAGG



 


13B
TTCTTCAGTCACGTGCCTGCGATTTCTAGTGT 
39



GGGAAACAGACCTGCGCCACCC



 


14B
TTCCCAGGAAGATCAGAGGCCCCACGGTGAT
40



CTCTGCTTCTTCAGTCACGTGCC



 


15B
AGAGGTTGACCCCTCTATACCATGATCACTA
41



GCCTTTCCCAGGAAGATCAGAGG



 


16B
CCAGGCCTAAGCCCAACATCACAGAGGTGTG
42



AGGAGAGGTTGACCCCTCTATAC



 


17B
CCAGGACAATGGTAGCTAGAGTCAGGGATAC 
43



CACCGTGGCCAGGCCTAAGCCCA



 


18B
GGGTGGGAAGCAGTACGATGCCTCTTGGCAA 
44



GGACCAGGACAATGGTAGCTAGA



 


19B
CGGTACCTTATTGGGAGACAGATGCAGGGCA 
45



TATCACAGGGTGGGAAGCAGTAC



 


20B
GACGGCGGTACCTTATTGGGAGAC
46









After synthesis of the dog ZP3 gene, it was cloned into the pTMF construct (ERA full genomic cDNA construct, see PCT Publication No. WO 2007/047459) at the P-M intergenic region for virus recovery. Four recombinant ERA-dogZP3 viruses (ERAZP3, ERAg3ZP3, ERA2g3ZP3 and ERAZP3T; see FIG. 1) were recovered by an established reverse genetics system for vaccine studies (PCT Publication No. WO 2007/047459). ERAZP3 contains the ZP3 sequence and a wild-type ERA G protein coding sequence. ERAg3ZP3 and ERA2g3 contain the ZP3 sequence and one or two copies (respectively) of the G333 mutant glycoprotein coding sequence. ERAZP3T contains a truncated ZP3 and the wild-type ERA G protein coding sequence. Truncated ZP3 comprises a deletion of nucleotides 79 to 1044 of ZP3 (SEQ ID NO: 7).
The four recombinant ERA-dogZP3 virus strains grew like wild type ERA virus in both baby hamster kidney (BHK) and BSR cells (a clone of BHK-21 cells), except for ERA2g3ZP3, which grew slower in the first three rounds of infection, relative to wild type ERA virus. Primary neutralization test from infected mice showed that ERAZP3T produced neutralizing antibody (NA) titer as high as 714.
In order to express the dog ZP3 gene in both prokaryotic and eukaryotic systems for immunologic studies, dog ZP3 was cloned into the pEF vector (for mammalian cell expression; Invitrogen) and pET28 vector (for prokaryotic expression; Novagen). Primary data by indirect fluorescence assay (IFA) showed that dog ZP3 was expressed well in BSR cells, demonstrated by His-tag monoclonal antibody staining.
The results of in vitro and in vivo studies using rabies virus ERA-based dog ZP3 recombinant virus are summarized as follows. ERAZP3 virus grew to 109 focus forming units (FFU)/ml in bioreactors, and replicated as well as parental ERA. Dog ZP3 was expressed as a non-structural protein in the purified ERAZP3 virion. ERAZP3 rabies virus, grown to 109 FFU/ml in BSR cells, was purified by gradient ultra-centrifugation. The purified recombinant virus was analyzed by SDS-PAGE. Five viral structural protein bands were clearly shown. The ZP3 protein was expressed as a nonstructural protein in recombinant ERAZP3 rabies virus. To detect ZP3 antibodies in ERAZP3 virus-immunized mice, Western blots using pcDNA/ZP3 expression protein were performed. BSR cells were transfected with pcDNA/ZP3 plasmids. After 48 hours, the transfected BSR cells were harvested and lysed. The supernatants were analyzed by SDS-PAGE, followed by protein transfer to nitrocellulose membranes. A standard Western-blot protocol was applied for analysis. The specific protein band with a molecular weight of 50 kD was detected, which corresponds to the size of ZP3.
In a mouse model, ERAZP3 induced a strong immune response against rabies virus when administrated either intramuscularly or orally. The immunized mice were protected against virus challenge, while the controls succumbed. Dog ZP3 antibodies were detected by indirect fluorescent staining Approximately 60 mice were injected intramuscularly with 50 μl of the recombinant virus (5×106 FFU per mouse). The mice were boosted at intervals of 7, 14 and 28 days. Rabies virus antibody response was evaluated. Rabies virus neutralization antibodies were very high, reaching more than 5 IU. The mice were euthanized and sera were collected for IFA and Western blot against ZP3 proteins. Positive results were observed in both tests.
In a hamster model, ERAZP3 administered intramuscularly induced a strong immune response against rabies virus. The immunized hamsters were protected when challenged. Dog ZP3 antibodies were detected by IFA. No adverse effects were observed in either mouse or hamster models.
Example 2
Rabies Virus ERA-Based Immunocontraceptive Pilot Studies Using GnRH
This example describes the development and testing of recombinant rabies viruses containing the gonadotropin-releasing hormone (GnRH) sequence inserted at various positions relative to the rabies virus glycoprotein (G).
GnRH has been proven to be efficient as an immunocontraceptive peptide for dogs. However, previously it has been necessary to link GnRH with a carrier protein (or adjuvant) to be immunogenic. The scale-up of the products to meet massive vaccination and quality control makes the synthetic chemical method unacceptable for commercial applications.
Through peptide analysis in vitro, appropriate positions for incorporation of GnRH into the glycoprotein can be applied for recombinant vaccine studies. There is no need for adjuvant because of the super-antigen-like properties of rabies virus particles. Since rabies virus grows efficiently in cell culture, scale-up of production is not limiting. Therefore, rabies virus engineered to include GnRH is an ideal candidate for simultaneous control of rabies and dog populations.
The GnRH peptide was tested in vitro to be immunogenic against rabbit anti-GnRH serum. Multiple locations in the rabies virus glycoprotein were chosen for insertion of the GnRH sequence (SEQ ID NO: 47) (see FIG. 2). The N terminus, antigenic site IIa, and the junction between the ectodomain and cytoplasmic domains were identified as ideal insertion sites for virus recovery. All recombinant viruses were recovered through an established reverse genetics system (PCT Publication No. WO 2007/047459). Rescued viruses were named ERA-N-GnRH, ERA-IIa-GnRH, and ERA-C-GnRH, according to the GnRH insertion site. These three viruses replicated as well as the parental wild type ERA, reaching titers of 109 FFU/ml in cell culture, with the exception of the ERA-IIa-GnRH virus. The inserted GnRH was stable in the glycoprotein gene after virus passage. Preliminary experiments in dogs using intramuscular administration demonstrated sufficient immune responses against rabies with no detectable adverse effects.
To increase the immunogenicity of the GnRH peptide, two copies of the GnRH gene aligned in tandem were cloned to the N (ERA-N-2GnRH) and IIa (ERA-GnRH-p3) sites. In the ERA-N-GnRH virus, the GnRH sequence (SEQ ID NO: 47) was inserted immediately after the 19 amino acid signal sequence of the rabies virus glycoprotein. The nucleotide and amino acid sequence of ERA-N-GnRH are set forth as SEQ ID NOs: 49 and 50, respectively. To create ERA-N-2GnRH, two copies of the GnRH in tandem were inserted immediately after the 19 amino acid signal sequence of the rabies virus glycoprotein (SEQ ID NOs: 51 and 52). To generate ERA-GnRH-p3, the GnRH sequence was inserted after amino acid residue 221 (IIa antigenic site) in rabies virus glycoprotein (SEQ ID NOs: 53 and 54). All three viruses were successfully recovered by reverse genetics, and the GnRH gene was stably expressed in all the constructs by Northern-blot. In addition, all of the constructs grew as well as parental rabies virus, with the exception of ERA-GnRH-p3, with grew slower. The ERA-N-GnRH virus was tested in dogs after intramuscular injection with no adverse effects. These results demonstrate that the N-terminus, just after the signal sequence in rabies virus glycoprotein, is an ideal location for insertion of GnRH.
To determine whether recombinant rabies viruses comprising GnRH are capable of eliciting protective immunity against rabies virus infection, wild-type rabies virus challenge studies were performed. Mice were injected i.m. with 5×105 FFU of either ERA-N-GnRH, ERA-3-GnRH(N-G3-GnRH-P-M-L) or ERA-G3-2GnRH(N-G3/2GnRH-P-M-L) and subsequently challenged with a lethal dose of rabies virus (FIG. 3). All vaccinated animals survived rabies virus challenge. In contrast, none of the control mice (unvaccinated naïve mice) survived rabies virus challenge. These results demonstrate that recombinant rabies virus-based immunocontraceptive vaccines are effective at eliciting a protective rabies virus immune response in animals.
Example 3
Combined Vaccines for Rabies Virus and Immunocontraception
This example describes the construction and characterization of recombinant ERA rabies viruses encoding GnRH.
Materials and Methods
Synthesis and Conjugation of GnRH Peptide to Keyhole Limpet Hemocyanin (KLH)
The decapeptide of GnRH (peptide 1780, GnRH; SEQ ID NO: 55), and two copies of the GnRH in tandem (peptide 1781, 2GnRH; SEQ ID NO: 56) were synthesized chemically, and purified by high performance liquid chromatography (HPLC). After verification, peptides 1780 and 1781 were conjugated to KLH. KLH was purchased from Sigma-Aldrich (St. Louis, Mo.) and conjugation efficiency was analyzed through SDS-PAGE. Protein Marker SeeBlueTM and Marker 12 were purchased from Invitrogen (Carlsbad, Calif.). The Precision Plus protein ladder was obtained from Bio-Rad (Hercules, Calif.). The proteins were separated on 4-12% SDS-PAGE gels.
Relocation of the G Gene Ahead of the P Gene in the RV ERA Genome and Pathogenicity of the Rearranged Virus
The rearranged RV ERA genome with the G gene relocated ahead of the P gene was constructed similarly to the previously described reverse genetics method (Wu and Rupprecht, Virus Res. 131: 95-99, 2008; Wu et al., Virus Res. 129: 91-103, 2007). The amino acid residue at position 333 (corresponding to residue 352 of SEQ ID NO: 5) of the RV G was changed from arginine (AGA) to glutamic acid (GAG) through mutagenesis (Wu et al., J. Virol. 76: 4153-61, 2002). The engineered virus was named ERAg3p. The growth characteristics of the mutated virus were determined in cell culture. BSR cells (a clone of BHK cell line) were grown in Dulbecco's minimal essential medium supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, Ga.). RV ERAg3p-infected BSR cells were incubated at 34° C., in a 5% CO2 incubator. The CELLine-1000 Bioreactor was from INTEGRA Bioscience AG (Switzerland). The stability of mutation at the defined position and the rearranged RV genome were verified through reverse transcription (RT)-polymerase chain reaction (PCR) by more than 100 continuous passages of infection in BSR cells. RV ERA or ERAg3p was injected intracerebrally (i.c) into ten three-week old ICR female mice (Charles River Laboratory). Ten healthy mice of the same species and age served as uninfected controls with injection of PBS buffer (0.01M, pH 7.4) by the same route. The virulence of RV ERAg3p was compared in parallel with that of parental ERA species. Animals were checked and recorded daily for signs of illness. Sick animals were euthanized by CO2 intoxication, followed by cervical dislocation. The mouse brain was removed for RV diagnosis.
Insertion of the Coding Sequence of GnRH into Various Locations of the G Gene in RV ERAg3p Virus
The coding sequence of GnRH (or 2GnRH) was inserted into 6 different locations of the G gene in RV ERAg3p. The G gene with the defined mutation in RV ERAg3p was denoted as G*. The primer sequences used for insertion of the GnRH or 2GnRH into the G* are shown in Table 2. Mutagenesis was performed as described previously (Wu and Rupprecht, Virus Res. 131: 95-99, 2008). The final 12 G* gene constructs were verified by sequencing using the ABI 3730 DNA Analyzer.







TABLE 2







Primers for insertion of GnRH or 2GnRH into G*













SEQ ID


Insert
Primer
Sequence
NO:





GnRH
GNRH15
CCAACCTGTCAGGGTTCTCCGAACA
58



(Forward)
CTGGAGCTACGGTTTGAGACCCGGG





TACATGGAACTTAAAGTTG



 


GnRH
GNRH13
GGAGAACCCTGACAGGTTGGTGCAT
59



(Reverse)
CCTTCGTCCTCCAC



 


2GnRH
2GNRHN5
GGTTTTTCCATTGTGTTTTGGGGAAC
60



(Forward)
ACTGGAGCTACGGTTTGAGACCCGG





GGAACACTGGAGCTACGGTTTGAGA





CCCGGGAAATTCCCTATTTACACG



 


2GnRH
2GNRHN3
CCCAAAACACAATGGAAAAACCAG
61



(Reverse)
AAGGGGTACAAACAGG









Recovery and Characterization of the GnRH-Carrying ERAg3p Viruses

The 12 constructs with GnRH (or 2GnRH) in-frame fused to the G* gene were applied for virus recovery following a previous reported protocol (Wu and Rupprecht, Virus Res. 131: 95-99, 2008; Wu et al., Virus Res. 129: 91-103, 2007). If virus could not be rescued in the first round transfection, two additional trials were repeated. A negative result by direct fluorescent assay (DFA) was interpreted as an indication of a non-optimal site in the G gene for GnRH insertion. The rescued viruses were further grown in the BSR cells to high titers using bioreactor incubation for characterization.
Expression of GnRH in RV ERAg3p Viruses
Total RNA from the GnRH-carrying ERAg3p virus-infected BSR cells was extracted using TRIZOLTM Reagent (Invitrogen, Carlsbad, Calif.). Digoxigenin (Dig)-labeled antisense oligonucleotide GnRH probe was synthesized according to standard methods. The Dig nucleic acid detection kit was purchased from Roche (Roche Diagnostics GmbH, Roche Applied Science, Penzberg, Germany). The protocol for Northern blotting has been previously described (Wu and Rupprecht, Virus Res. 131: 95-99, 2008; Wu et al., Virus Res. 129: 91-103, 2007; Wu et al., J. Virol. 76: 4153-61, 2002). The RNA molecular weight marker 1 was obtained from Roche (Roche, Indianapolis, Ind.). The procedure for purification of RV from infected cell culture supernatants was modified from previous descriptions (Thomas et al., Virology 25: 271-275, 1965; Sokol et al., J. Virol. 2: 836-849, 1968). Briefly, about 200 ml of virus supernatant from cell culture was filtered (0.22 μm pore diameter) to remove possible cell debris. The virions were pelleted through ultra centrifugation at 22,500×g for 1 hour (Beckman, SW 28). The pellet was resuspended overnight at 4° C. in 2 ml of 0.5 mM Tris buffer (pH 7.2), and was loaded to sucrose gradients for centrifugation at 24,000×g for 1 hour (Beckman, SW 41). The virus band in the gradient was collected for SDS-PAGE analysis. The pre-stained protein molecular weight standard was purchased from GIBCO (Carlsbad, Calif.).
Safety and Potency Against Rabies Using the GnRH-Carrying RV ERAg3p Viruses in a Mouse Model
Three-week old ICR female mice (Charles River Laboratory) were divided into four groups of 10 animals each. Group 1 was inoculated with RV ERA-N-2GnRH, group 2 with ERA-N-GnRH, group 3 with ERA-IIa-GnRH, and group 4 (as control) with PBS buffer (0.01 M, pH 7.4). Per mouse, 50 μl of each virus (6.0×106 FFU) or PBS buffer (0.01 M, pH 7.4, the controls) was injected intramuscularly (i.m) in the gestrocnemius muscle in the left leg. Three weeks after inoculation, surviving animals were challenged i.m by the same route in the right leg with a lethal dose of 50 μl of about 2.5-10.0 MICLD50 dog/coyote street RV (MD5951). The safety and potency of the viruses for the animals was analyzed two months after challenge.
Reaction of Serum from Immunized Mouse Using the GnRH-Carrying RV ERAg3p Viruses Against GnRH-KLH and 2GnRH-KLH Conjugates
Ten 3-week old ICR female mice (Charles River Laboratory) were immunized i.m in the gestrocnemius muscle of the left leg with 50 μl (6.0×106 FFU) of ERA-N-2GnRH, ERA-N-GnRH or ERA-IIa-GnRH. Three weeks post-vaccination, serum was collected by the retro orbital route after sedation of the animals. Serum was maintained at −20° C. for further analysis. The GnRH-KLH and 2GnRH-KLH conjugates were separated on 4-12% SDS-PAGE gels, and were transferred to polyvinylidene diflouride (PVDF) membrane (Sigma-Aldrich, St. Louis, Mo.) for Western blotting against the immunized mouse serum. Briefly, after gel electrophoresis, GnRH-KLH and 2GnRH-KLH were transferred to the PVDF membrane for blocking in 1× casein buffer (Vector Laboratories Inc, Burlingame, Calif.) at room temperature for 30 minutes. The immunized mouse serum (1:200 dilution in 1× casein reagent) was incubated with the membrane at room temperature for 30 minutes. After three washes (3 minutes each) in 1× casein Tris buffer, biotinylated anti-mouse IgG (H+L) (Vector Laboratories Inc, Burlingame, Calif.) at 1:1000 was added for another incubation of 30 minutes at room temperature. The staining kit was the ABC system from Vector Laboratories Inc. (Burlingame, Calif.).
Reaction of GonaConTM Immunized Rabbit Serum Against the GnRH-Carrying RV ERAg3p Viruses
GonaConTM immunized rabbit serum was obtained from the National Wildlife Research Center, USDA. The indirect fluorescent assay (IFA) for detection of GnRH peptide in recombinant RV-ERAg3p viruses was performed as follows. In one six-well-plate (Becton Dickinson Labware, N.J.), the ERA-N-2GnRH, ERA-N-GnRH or ERA-IIa-GnRH virus-infected BSR cells (37° C. for 48 h) were fixed in 4% formalin PBS (Protocol Formalin®, Fisher Scientific Company LLC, Kalamazoo, Inc) at room temperature for 30 minutes. The GonaConTM immunized rabbit serum at a dilution of 1:200 in PBS (0.01 M, pH 7.4) was added to the fixed BSR cells, and incubated at 37° C. for 30 minutes. After three washes in the same PBS (3 minutes each), the FITC-conjugated goat anti-rabbit IgG (H+L) at 1:200 dilution (Vector Laboratories Inc, Burlingame, Calif.) was added, and incubated at 37° C. for 30 minutes. The staining results were recorded under UV microscopy. For Western blot using the GonaConTM immunized rabbit serum against purified GnRH-carrying RV ERA viruses, the same protocol described above was followed.
Results
Synthesis and Conjugation of GnRH Peptide to KLH
The GnRH peptide (in bold): NH2—CEHWSYGLRPG-COOH (SEQ ID NO: 55), and 2GnRH peptide (in bold): NH2-CEHWSYGLRPGEHWSYGLRPG-COOH (SEQ ID NO: 56) were synthesized with an extra cysteine (C, italic in the sequence) at the amino terminus. The purity of peptides 1780 and 1781 were verified using Micro HPLC and MALDI mass spectrometric analyses. KLH was then conjugated through the extra amino terminal C residue to the 1780 and 1781 peptides. The conjugation efficiency was verified through SDS-PAGE (FIG. 4).
Growth Characteristics and Pathogenicity of the Rearranged RV ERAg3p
The rearranged ERA genome with the G gene relocated ahead of the P gene was constructed similarly to the previously described method of Wu et al. (Virus Res. 129: 91-103, 2007). Mutagenesis of the G gene at amino acid residue 333 from AGA to GAG was described elsewhere (Wu and Rupprecht, Virus Res. 131: 95-99, 2008). The recovered ERAg3p grew as well as parental ERA virus, reaching 4.2×109 FFU/ml in infected BSR cells in bioreactor incubation (FIG. 2B). Intracranial inoculation of the ERAg3p into 3-week old mice did not cause any signs of rabies, or other adverse side-effects. However, parental ERA virus killed all the mice inoculated by the same route (FIG. 2C). Therefore, the attenuated ERAg3p virus was used as a backbone for subsequent insertion of the GnRH coding sequence in immunocontraceptive studies.
Insertion of the Coding Sequence of GnRH into Various Locations of the G Gene in RV ERAg3p Virus
Six locations of the G* gene in RV ERAg3p were selected for insertion of GnRH coding sequence based upon previously identified antigenic epitopes: immediately after signal sequence; antigenic site II; antigenic site IIa; antigenic site WB+; antigenic site III; and the junction between the ecto- and transmembrane domains (see FIG. 6) (Coulon et al., J. Gen. Virol. 64: 693-696, 1983; Seif et al., J. Virol. 53: 926-934, 1985; Prehaud et al., J. Virol. 62: 1-7, 1988). The coding sequence for GnRH (GAACACTGGAGCTACGGTTTGAGACCCGGG; SEQ ID NO: 47) was introduced into the above 6 locations through mutagenesis. The 2GnRH coding sequence linked in tandem (GAACACTGGAGCTACGGTTTGAGACCCGGGGAACACTGGAGCTACG GTTTGAGACCCGGG; SEQ ID NO: 57) was also incorporated into the G* gene in a similar way. The final 12 G* gene constructs were verified by DNA sequencing, and were successfully cloned into the RV ERAg3p full length plasmid for virus recovery. The nucleotide and amino acid sequences of the four G* gene constructs that were recovered in recombinant rabies viruses (see Table 3) are set forth as SEQ ID NOs: 49 and 50 (G-N-GnRH); SEQ ID NOs: 51 and 52 (G-N-2GnRH); SEQ ID NOs: 53 and 54 (G-IIa-GnRH); and SEQ ID NOs: 63 and 64 (G-C-GnRH).
Recovery and Characterization of the GnRH-Carrying ERAg3p Viruses
Each of the 12 G* constructs (FIG. 6) with GnRH or (2GnRH) in-frame fused to the G gene was successfully cloned ahead of the P gene in the RV ERAg3p genome. The full-length sequence of each construct was confirmed to be correct before virus recovery. Recombinant virus was successfully recovered from 4 out of the 12 constructs in which the GnRH was inserted at amino terminus immediately after the signal sequence (the recovered virus was named RV ERA-N-GnRH or ERA-N-2GnRH), IIa site (RV ERA-IIa-GnRH), or the junction between the ecto- and transmembrane domains (RV ERA-C-GnRH) of the glycoprotein (see Table 3 below). Plasmid transfection tests for virus rescue were repeated in two separate trials if no virus was detected in the first round of recovery. The recovered RV ERA-N-GnRH, ERA-N-2GnRH and ERA-C-GnRH grew well in cell culture, but the ERA-IIa-GnRH virus did not grow efficiently, and the titer was about 100 times lower than its counterparts (FIG. 7B).







TABLE 3







Recovery of GnRH-carrying ERAg3p viruses









Virus construct
G gene construct
Virus recovered





ERA-N-GnRH
G-N-GnRH
Yes


ERA-N-2GnRH
G-N-2GnRH
Yes


ERA-II-GnRH
G-II-GnRH
No


ERA-II-2GnRH
G-II-2GnRH
No


ERA-IIa-GnRH
G-IIa-GnRH
Yes


ERA-IIa-2GnRH
G-IIa-2GnRH
No


ERA-WB + GnRH
G-WB + GnRH
No


ERA-WB + 2GnRH
G-WB + 2GnRH
No


ERA-III-GnRH
G-III-GnRH
No


ERA-III-2GnRH
G-III-2GnRH
No


ERA-C-GnRH
G-C-GnRH
Yes


ERA-C-2GnRH
G-C-2GnRH
Not tested









Expression of GnRH in the RV ERAg3p Viruses

The GnRH inserted between the ecto- and transmembrane domains of the G protein may not be in an optimal position for exposure to the virus surface. Thus, the following studies described herein focused on RV ERA-N-2GnRH, ERA-N-GnRH and ERA-IIa-GnRH. Through SDS-PAGE of purified viruses, a typical 5-band pattern was stained by Coomassie blue (FIG. 8A). The G protein bands from RV ERA-N-GnRH and ERA-N-2GnRH were excised from the gel for protein sequence analysis. The amino terminus of the G protein was verified to be blocked after fusion to the GnRH peptide in three independent trials. However, GnRH was detected in the fused G mRNA using Northern-blot in both ERA-N-2GnRH and ERA-N-GnRH viruses (FIG. 8B).
Safety and Potency Against Rabies Using the GnRH-Carrying RV ERAg3p Viruses in a Mouse Model
No obvious side-effects or behavior changes were observed in mice inoculated with RV ERA-N-2GnRH, ERA-N-GnRH or ERA-IIa-GnRH. Surviving animals were challenged 3 weeks post-inoculation with a lethal dose of about 2.5-10.0 MICLD50 dog/coyote street RV. All control mice developed typical rabies signs, and were euthanized between 8 and 10 days. RV antigen was detected in the brain by DFA. The surviving mice in the GnRH-carrying RV ERAg3p groups did not develop any signs of rabies, and remained healthy before termination of the experiment in 2 months (FIG. 9).
Reaction of Immunized Mouse Serum Using the GnRH-Carrying RV ERAg3p Viruses Against GnRH-KLH and 2GnRH-KLH Conjugates
To compare the reactivity of immunized mouse serum using the GnRH-carrying RV ERA viruses with that of GonaConTM immunized rabbit serum (from the USDA) against GnRH-KLH and 2GnRH-KLH, the peptide conjugates were separated on 4-12% SDS-PAGE gels. In Western blotting, both GnRH-carrying RV ERA immunized mouse serum and GonaConTM immunized rabbit serum recognized the GnRH-KLH and 2GnRH-KLH conjugates (FIG. 10). However, each conjugate presented several bands in serology, indicating an un-unified or uncontrollable process in peptide linkage.
Reaction of GonaConTM Immunized Rabbit Serum Against the GnRH-Carrying RV ERAg3p Viruses
In the IFA, typical cell membrane florescence was observed in the ERA-N-2GnRH, ERA-N-GnRH and ERA-IIa-GnRH infected BSR cells. The staining pattern was compatible with that of rabies G protein in RV-infected cells. In the Western blot using purified virus against GonaConTM immunized rabbit serum, the G protein band was stained, which is an indication of fusion of the GnRH peptide with RV glycoprotein.
Example 4
In Vivo Studies of ERA-GnRH in Canines
This example describes the testing of ERA-GnRH vaccine constructs (such as those disclosed herein) in dogs to establish safety and efficacy. Recombinant ERA-GnRH virus will be tested in dogs for dual evaluation of rabies efficacy and immunocontraceptive effects for population control. It is hypothesized that ERA-GnRH will elicit rabies virus neutralizing antibody and stabilize the population of the immunized dogs within 3 years after one dose. ERA-GnRH will be administered to approximately 100 dogs (50 male and 50 female) and another 20 dogs will serve as controls. Recombinant rabies viruses will be administrated intramuscularly at a dose of approximately 107 FFU/ml, or will be administered orally at a dose of approximately 108 FFU/ml. It is believed that around 70% of the immunized animals will remain sterile for a year, and the litter number will drop at least 50%.
Example 5
Vaccination of Dogs with a Rabies Virus-Based Immunocontraceptive
This example describes a rabies virus-based immunocontraceptive vaccination study to be carried out on rabies virus naïve dogs. Seven groups of stray, fully reproductive adult, rabies naïve dogs will be included in this experiment. The absence of rabies virus neutralizing antibodies (VNAs) in serum will be used to corroborate that the animals are rabies naïve. Groups will consist of 20 animals, each with a 1:1 male to female ratio to ensure that statistical significance for males and females within each group is achievable. Pregnancy will be ruled out before the start of the experiment. In addition, canine transmissible venereal tumor must be discarded in both males and females. All animals will be quarantined (at least 40 days) and undergo mandatory full de-worming.
Two groups (20 animals each) will be vaccinated with 1 mL of recombinant rabies virus (as disclosed herein) on day 0, and administered a single booster on day 21. One group will be vaccinated intramuscularly (i.m) and the other group orally. Two other groups (20 animals each) will be vaccinated with a single dose of 1 mL of recombinant rabies virus by i.m or oral administration on day 0. Control groups (20 animals each) will receive placebo (cell culture media, the same that was used in the virus propagation) intramuscularly or orally (by instillation). A third group, the contraception control group, will receive GonaConTM (a GnRH immunocontraceptive vaccine) by i.m. injection. All groups will be labeled accordingly (such as by using different color collars or with a tattoo indicating the group number). The test and control groups are summarized below.

    Group 1: 20 animals (10 males and 10 females) inoculated with 1 mL of construct by i.m. route, at day 0 and 21.
    Group 2: 20 animals (10 males and 10 females) inoculated with 1 mL of construct by oral route, at day 0 and 21.
    Group 3: 20 animals (10 males and 10 females) inoculated with 1 mL of construct once, i.m. route at day 0.
    Group 4: 20 animals (10 males and 10 females) inoculated with 1 mL of construct once, oral route at day 0.
    Group 5: 20 animals (10 males and 10 females) inoculated with 1 mL of cell culture media by i.m. route.
    Group 6: 20 animals (10 males and 10 females) inoculated with 1 mL of cell culture media by oral route.
    Group 7: Contraception control group with 20 animals (10 males and 10 females) inoculated with 1 mL of GonaConTM by i.m. route.

Caging



For confinement purposes, big cages or kennels (e.g., 5 meters×5 meters) will be used to confine up to 10 dogs each. Males and females will be separated at all times to avoid fighting among males when females are in heat. In addition, the kennels or cages will be sufficient to protect all dogs from sun and rain. Fresh water will be available all the times.
Sampling Schedule and Monitoring
Serum samples will be taken from vaccination candidates for screening purposes (up to 200 or more dogs will be tested if necessary) in order to select the 140 appropriate animals (dogs of both genders in reproductive age) with no anti-rabies antibodies (see Table 4).
Serum samples will be taken from all 120 dogs (groups 1 to 6) every week during the entire experiment (days 0, 7, 14, 28, and if possible, 6 months later) to measure the titers of VNA and immunocontraceptive responses.
Contraception Challenge
Animals in all groups will mate with healthy reproductive adults. Ideally, in groups 3 and 4, mating will occur 4 weeks after vaccination (day 28). For animals that receive a booster immunization at day 21, animals should mate between 14 to 21 days after the booster. One healthy stud will be used for every five bitches. Males in placebo control groups can be used as studs for vaccinated dogs and female dogs in these groups will also be mated.







TABLE 4







Schedule Prior to the Study (Weeks 1-8)









Time in Weeks















Activity
1
2
3
4
5
6
7
8





Recruiting process
X
X
X







(gathering dogs, potential


candidates)


Pregnancy and CTVT tests
X
X
X


De-worming
X
X
X

X


Preventive vaccinations
X
X
X


Preventive vaccinations and




X


treatment booster


Bleeding
X
X
X


Shipping sera samples to CDC



X


Quarantine
X
X
X
X
X
X
X


Detection of RVNA at CDC,





X
X


screening process


Selection of 120 animals about







X


50% males and 50% females
















TABLE 5







Schedule for the Study (Weeks 9-24 and up to 6 months)









Time in Weeks





































Up to 6


Activity
9
10
11
12
13
14
17
18
19
20
21
22
23
24
months





Immunization with
X
















RABV1 constructs


group 3 and 4


Inoculation of
X


placebo to groups 5


and 6


Bleeding for all
X
X
X
X
X
X
X
X
X
X


groups, serum


separation and


storage at −20° C.


Booster with



X


RABV constructs


groups 1 and 2


Shipping sera




X



X



X


samples to CDC2


Detection of
X
X
X
X
X
X
X


RVNA3 at CDC


Fertility test for
X
X
X
X
X
X
X
X

X




X


both genders


Mating



X
X

X
X





X
X


Pregnancy tests







X
X

X
X





1Recombinant rabies virus;


2Centers for Disease Control and Prevention;


3Rabies virus neutralizing antibody






It is anticipated that approximately 70% of the immunized animals will remain sterile for a year, and the litter number will drop at least 50%. It is further believed that more than 80% of the animals will survive lethal doses of rabies virus challenge at the end of the study.
Example 6
In Vivo Safety, Immunogenicity and Efficacy Evaluation of Recombinant Rabies Virus Immunocontraceptive Vaccines in a Rodent Model
The first phase of this study will test the efficacy of the rabies virus immunocontraceptive (GnRH) vaccines against rabies virus infections in mice. Twenty 4-week old mice will be divided into groups of males (n=10) and females (n=10) (20 mice for each vaccine, GonaConTM and combination of vaccines and GonaConTM), and receive an experimental biologic on day 0 (50 μl via intramuscular injection into the left gastrocnemius muscle). On days 7, 14 and 28, blood will be collected from all mice by the submandibular collection technique and tested for the presence of rabies virus neutralizing antibodies (VNA), antibodies against GnRH, and testosterone and estrogens. Mice with detectable levels of rabies virus neutralizing antibodies will be challenged with rabies virus in the right gastrocnemius muscle on day 28 after vaccination. Animals will be euthanized at the first clinical signs of rabies. Brain and reproductive organs will be collected for histological examination.
Groups: 1) live recombinant vaccine with 1-8 copies of incorporated GnRH (8×20 mice); 2) inactivated recombinant vaccine with incorporated GnRH (20 mice); 3) commercial vaccine (20 mice); 4) GonaConTM (20 mice); 5) live recombinant vaccine with incorporated GnRH (20 mice)+GonaConTM; 6) commercial vaccine+GonaConTM (20 mice); 7) inactivated recombinant vaccine with incorporated GnRH (20 mice)+GonaConTM; 8) control group administered PBS (10 mice).
Expected Outcome: By the end of a 3-month observation period, at least 80% of immunized animals are expected to survive without sign of rabies.
Example 7
Intramuscular Contraception Trial in Rodents
Vaccination will be conducted as described above. Each group will contain 10 mice of each sex. Animals will be bled on days 7, 14, and 28 after vaccination to measure VNA against rabies virus and GnRH, as well as progesterone in female mice and testosterone in male mice. Each mouse in the recombinant vaccine groups will be matched with a control mouse of the opposite sex (non-vaccinated, fertile) in new housing on day 30 (total 40 mice per group). These 20 pairs will be kept for observation. Females will be checked for pregnancy every 2 days following matching.
To measure longevity of induced immune responses and correlation with infertility, mouse pairs will be kept together for an additional 6 months (or until females are pregnant), if females do not become pregnant within the first 18 days. Mice will be bled via the submandibular route bi-weekly. Female sex organs will be examined for pregnancy after euthanasia.
Expected Outcomes: By the end of 3 months, at least 80% of females are expected not to be pregnant and at least 80% of males are expected not to impregnate non-immunized females. Serological responses will correlate with fertility ratios. Two or more recombinant rabies viruses will be selected for oral contraceptive investigations.
If efficacy (infertility in vaccinated animals of both sexes) is achieved by the intramuscular route, the immunogenicity and efficacy of the vaccine by oral administration will be evaluated. Experimental design will be similar to the i.m. contraception trial.
Example 8
In Vivo Immunogenicity and Safety Study in a Dog Model
Efficacy trial (intramuscular administration): Efficacy of the recombinant immunocontraceptive vaccines against rabies virus infections and their ability to induce immune responses against the GnRH will be tested in male and female dogs. Each group will consist of 8 animals (4 males and 4 females). In the first phase, various selected vaccines, proven to be efficacious and immunogenic in rodent model, will be administered i.m. Blood will be collected on day 0 and subsequently once or twice a week for the first two months and monthly thereafter. Serum will be tested for the presence of rabies virus neutralizing antibodies and antibodies against GnRH. Levels of GnRH, progesterone and testosterone also will be measured. A control group of 4 dogs will receive a placebo injection. Four animals in each group (previously vaccinated with one of the generated rabies vaccine constructs with proven titer of rabies virus neutralizing antibodies) will be inoculated with rabies virus in the right gastrocnemius muscle on day 28 after vaccination. Animals will be observed and euthanized (intravenous injection of a barbituric acid derivative) at the first clinical signs of rabies. Brain and reproductive organs will be collected for histological examinations. Design of experimental groups will depend upon results from trials of these vaccines in rodent models. Given previous vaccination, survival of all experimental animals is expected.
Groups (8 Dogs Each): 1) live recombinant vaccine with incorporated GnRH; 2) inactivated recombinant vaccine with incorporated GnRH; 3) commercial vaccine; 4) GonaConTM; 5) rabies vaccine+GonaConTM; 6) APHIS/NWRC recombinant GnRH-VLP; and 7) control group (4 dogs). Phase 1 of the immunocontraceptive vaccine experiment would require a maximum of 52-60 animals. Depending upon the results of the safety, immunogenicity, and efficacy experiments with the vaccines administered i.m., oral administration of selected live attenuated vaccines with incorporated GnRH will be tested as well.
Expected Outcomes: By the end of a 1 year observation period, at least 80% of immunized animals are expected to survive without any sign of rabies, and at least some experimental groups are expected to have significant titers of anti-GnRH antibodies and significantly decreased levels of progesterone and testosterone.
Contraception Trial in Dogs: Efficacy of the best experimental vaccine with incorporated GnRH, proven immunogenic in efficacy trials above in rodents and dogs, will be tested for its ability to induce infertility in female dogs following intramuscular administration. The treated and control groups will consist of 10 and 5 animals, respectively.
Expected Outcomes: By the end of a 1 year observation period, at least 80% of immunized animals are expected to remain infertile, with significant titers of anti-GnRH antibodies and decreased levels of progesterone and testosterone. At least 50% of control animals are expected to successfully breed.
This disclosure provides recombinant rabies viruses comprising immunocontraceptive proteins. The disclosure further provides methods of simultaneously protecting non-human animals from rabies virus infection and inhibiting fertility of the animal. It will be apparent that the precise details of the methods described may be varied or modified without departing from the spirit of the described disclosure. We claim all such modifications and variations that fall within the scope and spirit of the claims below.

## Claims

1. A recombinant rabies virus, wherein the genome of the recombinant rabies virus comprises rabies virus nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and RNA-dependent RNA polymerase (L) genes and a heterologous nucleic acid sequence encoding a gonadotropin-releasing hormone (GnRH) protein, wherein the heterologous nucleic acid sequence encoding the GnRH protein is at least 95% identical to SEQ ID NO: 47.

2. The recombinant rabies virus of claim 1, wherein the G gene is relocated between the N gene and the P gene in the genome of the recombinant rabies virus.

3. The recombinant rabies virus of claim 1, wherein the rabies virus glycoprotein comprises a Glu at amino acid position 333.

4. The recombinant rabies virus of claim 1, comprising two copies of the heterologous nucleic acid sequence encoding the GnRH protein.

5. The recombinant rabies virus of claim 1, wherein the heterologous nucleic acid molecule encoding the GnRH protein is inserted within the rabies virus glycoprotein gene.

6. The recombinant rabies virus of claim 5, wherein the heterologous nucleic acid sequence encoding the GnRH protein is inserted following the signal sequence (nucleotides 1-57 of SEQ ID NO: 49) of the glycoprotein gene.

7. The recombinant rabies virus of claim 6, wherein the glycoprotein gene comprises the nucleic acid sequence of SEQ ID NO: 49 or SEQ ID NO: 51.

8. The recombinant rabies virus of claim 5, wherein the heterologous nucleic acid sequence encoding the GnRH protein is inserted immediately following antigenic site Ha (nucleotide 663 of SEQ ID NO: 53) of the glycoprotein gene.

9. The recombinant rabies virus of claim 8, wherein the glycoprotein gene comprises the nucleic acid sequence of SEQ ID NO: 53.

10. The recombinant rabies virus of claim 5, wherein the heterologous nucleic acid sequence encoding the GnRH protein is inserted at the junction of the ectodomain and transmembrane domain (following nucleotide 1374 of SEQ ID NO: 63) of the glycoprotein gene.

11. The recombinant rabies virus of claim 10, wherein the glycoprotein gene comprises the nucleic acid sequence of SEQ ID NO: 63.

12. An immunogenic composition comprising the recombinant rabies virus of claim 1 and a pharmaceutically acceptable carrier, an adjuvant, or both.

13. A method of immunizing a non-human animal against rabies virus infection and inhibiting fertility of the animal, comprising administering to the animal a therapeutically effective amount of the immunogenic composition of claim 12.

14. The method of claim 13, wherein the animal is a dog, cat, rat, mouse, bat, fox, raccoon, squirrel, opossum, coyote or wolf.

15. The method of claim 13, wherein the immunogenic composition is administered orally.

16. The method of claim 13, wherein the immunogenic composition is administered through food-baits.

17. The method of claim 13, wherein the animal is a domestic animal.

18. The method of claim 13, wherein the animal is a wild animal.