OFFICE OF 

PREVENTION, PESTICIDES AND 

TOXIC SUBSTANCES

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

WASHINGTON D.C., 20460 

PC Code: 129008 

DP Barcodes: 418780, 401791, and 416755 

MEMORANDUM Date: August 20, 2015 

SUBJECT: Nicosulfuron Environmental Fate Study Data Reviews: 

Aerobic soil metabolism (OCSPP Guideline No. 835.4100) 

Batch Equilibrium/Adsorption, Desorption, Leaching (OCSPP Guideline No. 

835.1230) 

Soil ECM & ILV (OCSPP Guideline No. 850.6100) 

Water ECM & ILV (OCSPP Guideline No. 850.6100) 

TO: Miguel Zavala, Chemical Review Manager 

Kelly Sherman, Branch Chief 

Risk Management and Implementation Branch III 

Pesticide Re-evaluation Division (7508P) 

Kable Davis, Risk Manager (RM-25) 

Daniel Kenny, Risk Manager Reviewer 

Herbicide Branch

Registration Division (7505P) 

This memorandum transmits the Environmental Fate and Effects Division’s (EFED’s) data 

reviews for several environmental fate studies requested to support the Nicosulfuron Registration 

Review risk assessment. The study classifications are shown in Table 1 below. Data evaluation 

records (DERs) documenting EFED’s study review have been prepared for each study shown in 

Table 1 and accompany this memo. 

Please note that while the Environmental Chemistry Methods and Independent Laboratory 

Validations (ECMs and ILVs) in both soil and water for nicosulfuron were found acceptable 

(MRID Nos. 48790911, 487090913, 48790912, and 48790914) , there will still be a data gap for 

this study in the future considering that the level of detection (LOD) and level of quantitation 

(LOQ) established in the methods were not sufficient enough to encompass the low 
2

concentrations with toxicological endpoints determined for terrestrial plants and expected for 

aquatic plants with nicosulfuron. Further information related to LODs and LOQs established for 

other existing methods can be found within the DERs. 

Please contact Gabe Rothman at (703) 347-8011 with any questions. 
3

Table 1. Summary of data classifications of recently submitted studies for nicosulfuron. 

Guideline Study Citation MRID Study 

Classification 

Test 

Substance 

835.4100 Aerobic Soil Metabolism: 

Sarff, P. 2004. Rate of 14C-Nicosulfuron Degradation 

in Three Soils. Unpublished study performed by ABC 

Laboratories, Inc., Columbia, Missouri and DuPont 

Crop Protection, Newark, Delaware; sponsored and 

submitted by E.I. du Pont de Nemours and Company, 

Wilmington, Delaware. DuPont Study No.: 10929; 

ABC Study No.: 47547. Experimental work 

conducted between August 13, 2002 and November 

19, 2003 (p. 6). Final report issued April 1, 2004. 

49169703 Supplemental Nicosulfuron 

TGAI 

835.1230 Batch Equilibrium (Adsorption, Desorption, and 

Leaching) 

Aikens, P.J. 2001. Adsorption/Desorption of [14C]INJ0290 in Five Soils. Unpublished study performed by 

Huntingdon Life Sciences Ltd., Cambridgeshire, 

England; sponsored and submitted by E.I. du Pont de 

Nemours and Company, Wilmington, Delaware. 

DuPont Project ID DuPont-5264. Huntingdon Project 

ID DPT 564. Experimental initiation February 23, 

2001 and completion May 25, 2001 (p. 11). Study 

completion date November 2, 2001. 

49169701 Acceptable IN-J0290 

Mebabolite 

850.6100 ECM/ILV in Soil 

Cabusas, M.E.Y. 2012. Analytical method for the 

determination of nicosulfuron (DPX-V9360), INV9367 and IN-J0290 in soil using HPLC/ESIMS/MS. Laboratory Project ID: DuPont-33143. 

Report prepared by E.I. du Pont de Nemours and 

Company, Newark, Delaware, sponsored and 

submitted by E.I. du Pont de Nemours and Company, 

Wilmington, Delaware; 50 pages. Final report issued 

March 16, 2012. 

Rogers, P. 2012. Independent laboratory validation of 

DuPont-33143, “Analytical method for the 

determination of nicosulfuron (DPX-V9360), INV9367 and IN-J0290 in soil using HPLC/ESIMS/MS”. DuPont Project ID: DuPont-33031. Alliance 

Pharma Project No.: 120103. Report prepared by 

Alliance Pharma, Inc., Malvern, Pennsylvania, 

sponsored and submitted by E.I. du Pont de Nemours 

and Company, Wilmington, Delaware; 52 pages. Final 

report issued March 22, 2012.

48790912 

& 

48790914 

Acceptable* Nicosufuron,

IN-V9367 

Metabolite, 

and 

IN-J0290 

Metabolite 

850.6100 ECM/ILV in Water 

Morgan, E.A. and Cabusas, M.E.Y. 2012. Analytical 

method for the determination of nicosulfuron (DPXV9360), IN-V9367 and IN-J0290 in water using 

HPLC/ESI-MS/MS. Laboratory Project ID: DuPont32132. Report prepared by E.I. du Pont de Nemours 

and Company, Newark, Delaware; sponsored and 

submitted by E.I. du Pont de Nemours and Company, 

Wilmington, Delaware; 54 pages. Final report issued 

March 16, 2012.

48790911 

& 

48790913 

Acceptable* Nicosufuron,

IN-V9367 

Metabolite, 

and 

IN-J0290 

Metabolite 
4

Guideline Study Citation MRID Study 

Classification 

Test 

Substance 

Robaugh, D.A. 2012. Independent laboratory 

validation of DuPont-32132, “Analytical method for 

the determination of nicosulfuron (DPX-V9360), INV9367 and IN-J0290 in water by HPLC/ESIMS/MS”. DuPont Study No: DuPont-32578. Pyxant 

Labs Project ID: 2388. Report prepared by Pyxant 

Labs Inc., Colorado Springs, Colorado; sponsored and 

submitted by E.I. du Pont de Nemours and Company, 

Newark, Delaware; 92 pages. Final report issued 

March 30, 2012.

* While the Environmental Chemistry Methods and Independent Laboratory Validations (ECMs and ILVs) in both

soil and water for nicosulfuron were found acceptable, there will still be a data gap for this study in the future 

considering that the level of detection (LOD) and the level of quantitation (LOQ) established in the methods were 

not sufficient enough to encompass the low concentrations for toxicological endpoints determined for terrestrial 

plants and expected for aquatic plants with nicosulfuron. Further information related to LODs and LOQs 

established for other existing methods can be found within the DERs.