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                            Funding & Awards
 Mitochondrial DNA Analysis by Denaturing Liquid Chromatography for the Separation of Mixtures in Forensic Samples
Note:
              This awardee has received supplemental funding. This award detail page includes information about both the original award and supplemental awards.
Award Information
Awardee
              University of Denver
Award #
              2003-IJ-CX-K104
Funding Category
              Competitive
Location
                    Denver, CO
Awardee County
              Denver
Congressional District
              1
Status
              Closed
Funding First Awarded
              2003
Total funding (to date)
              $457,062
Original Solicitation
              National Forensic Sciences Improvement Act
Description of original award (Fiscal Year 2003, $202,513)
Project Summary  2003-ij-cx-k104

This award aims to demonstrate the reliability of DHPLC to detect and clearly resolve mixtures of mitochondrial DNA or all classes of mtDNA polymorphisms including c-stretch variants, insertion/deletion variants and sequence polymorphisms in both   GC -rich and AT- rich areas in the HVI and HVII region of the human mtDNA 2) to efficiently seperate and recover individual components of DNA mixture for subsequent analyisis by direct DNA sequencing and then to confirm the accuracy of the resulting sequence data and 3) with regards to the absence of cross-contamination between individual samples in the sample tray and between individual assays.
Supplemental Award 1 (Fiscal Year 2005, $254,549)
The first phase of this project examined the feasibility of DHPLC to successfully separate mtDNA mixtures that differed at one or more positions in the mtDNA hypervariable regions (HV1 and HV2). The grantee has demonstrated that DHPLC can successfully be used for this purpose. This supplemental project will develop Standard Operating Procedures and interpretation guidelines for mtDNA mixture analysis using DHPLC-separated fractions, and perform developmental validation studies in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM).
nca/ncf


  
Grant-Funded Publications
        
                      Comparative Analysis of the HV1 and HV2 Regions of Human Mitochondrial DNA by Denaturing High-Performance Liquid Chromatography
                  Validation of Reduced-Scale Reactions for the Quantifiler Human DNA Kit
                  Mitochondrial DNA Analysis by Denaturing Liquid Chromatography for the Separation of Mixtures in Forensic Samples

  
Date Created: September 11, 2003
    
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