## FROM: U. S. ENVIRONMENTAL PROTECTIONAGENCY Washington, D.C. 20460

OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION


## Date: February 22, 2011 

## Chemical: Pyraclostrobin 

## PC Code: 099100 

## DP Barcodes: 380640,380650,384006,384010,384012, and 385332 

### Dec.t"!1I07\ :**4~:2~"1) 4~41**  '05) 

### 4311.CJO 

## MEMORANDUM: SUBJECT: Ecological Risk Assessmentto Support the ProposedNew Use of Pyraclostrobin on Rapeseed (Cultivars, Varieties, and/or Hybrids, Including Canolaand Crambe), Sweet Com and Sugar Beet and as an Encapsulated Product on Field Com, Pop Com and Seed Production Com Lewis Ross Brown, Environmental Biologist

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# ,. , , RobertMiller, Environmental Protection Specia.list

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## THROUGH: Nancy Andrews, Branch Chief 

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### Sujatha Sankula, Lead Biologist

# '-'Y'-\~**,\_o-~** Environmental Risk Branch I**~;::::::::::::-='-** 

### Environmental Fate and Effects Division (7507P) 

## TO: Tony Kish, Risk Manager ShauntaHill, Risk ManagerReviewer Fungicide Branch Registration Division (7505P)


### 1. 

## Executive Summary
This ecological risk assessment evaluates the proposednew uses ofthe following pyraclostrobinproducts, Coronet seed treatment on rapeseed (cultivars, varieties, and/or hybrids, including canola and crambe), BAS 500 20 F on field com, pop com and seed productioncomand Stamina seed treatment on sweet com and sugar beet. This risk assessment focuses on the environmental fate and effects ofpyraclostrobin and not it's majordegradates due to the lack environmental fate and toxicity data for the major degradates.
The proposed maximum applicationrates for seed treatments are calculated to be 0.0096 lbs. a,ijA forrapeseed 0.003 lbs. a,ijA for sweet com and 0.11 lbs. a.i.zA for sugar beet.

1 11111111111111111Wlllllilim1111111111 

### 2086342

### The maximum ground/aerial applicationrate for BAS 500 20 F on corn is one application ofO.llbs. a.i./A per year.
The proposed seed treatment application rates are significantly lower than the maximum registered applicationrate of0.5 lbs. a.i./A (DP barcode: D269625). Consequently, in contrastto the currentlyregistereduses, the proposed seed treatments result in a significantlyreduced exposure ofnon-targetwildlife to pyraclostrobin environmental concentrations that are predictedto be several orders ofmagnitude belowthe Agency LOC. Also, the proposed encapsulated foliar applicationrate to corn is significantly less thanthe registered foliar applicationrate of0.5 lbs. a.i./A for corn on the Headline SC label (EPA Reg. No. 7969-289). Therefore, EFED has concludedthat a full screening risk assessment is not warranted. 

### Tables 1-4 lists the LOC exceedences for the proposed new uses ofpyraclostrobin.



### Table 1. 

## Summary ofLOC Exceedencesforthe Proposed New Use of PyraclostrobinWhen Used AsanEncapsulated Formulationin 

## Corn(Field, Pop, and Seed-production)

Taxonomic Group Effect Mamma Terres Terrest. Aquatic Aquatic Birds 

### Is t. Inverts Fish Inverts Plants Plants· DirectEffect LOC YesExceedences No Freshwater Yes-Salt- No No No fishes water Inverts No Indirect Effect Exceedances Yes Yes Yes Yes Yes Yes No 

### Table 2. Summary ofLOC Exceedences for the ProposedNew Use of Pyraclostrobin When Used As a Seed-treatmentoBSuear

## BeetSeeds Taxonomic Group Effect Mamma Terres Terrest. Aquatic Aquatic Birds 

### t. Fish Is Plants Inverts Inverts Plants DirectEffectLOC YesExceedences Yes Freshwater Yes No Yes No fishes No Indirect Effect Exceedances Yes Yes Yes Yes Yes Yes No 

### Table 3. Summary of LOC Exceedences for the Proposed New Use of Pyraclostrobin When Used As a Seed-treatmenton Sweet Corn Seeds Taxonomic Group Effect Mamma Terres Terrest. Aquatic Aquatic Birds t. Fish Is Plants Inverts Inverts Plants Direct EffectLOC Exceedences No No No No No No



### 2No Indirect Effect Exceedances Yes Yes Yes Yes Yes Yes No



### Table 4. Summary ofLOC Exceedences for the Proposed New Use ofPyraclostrobin When Used as a Seed-treatment on Canola and Carambe Seeds Taxonomic Group Effect 

### Mamma Terres Terrest. Aquatic Aquatic Birds 

### t. Fish 

### Is Plants Inverts Inverts Plants Direct Effect LOC Exceedences Yes Yes No Yes Yes No No Indirect Effect Exceedances Yes Yes Yes No Yes Yes No



### Risk Summary for This Assessment:
A screening-level (Level I) risk assessment suggests that pyraclostrobin as an encapsulated foliar treatmentuse on comand as a seed-treatmentuse on sweet com, sugar beets, and rapeseed providedthe following:

• Potential Acute risks to 20-grambirds consuming pyraclostrobin-treated canola seeds when applied as a seed treatmentto rapeseed as acute risk quotients for 20- gram avian species were exceeded at the Acute RestrictedUse and Acute Listed Species Use risk presumption categories (RQ=0.24)

• Potential Acute risks to 20-grambirds consuming pyraclostrobin-treated sugar beet seeds as acute risk quotients for 20-gram avian species were exceeded at the Acute Risk Use, Acute Restricted Use, and Acute Listed Species Use risk presumption categories (RQ=3.24)

• Potential Chronic risks to 20-grambirds consuming pyraclostrobin-treated sugarbeet seeds as chronic risk quotients for 20-gram avian species were exceeded at the chronic Level ofConcern (RQ=I2.54)

• Potential Acute risks to I5-gram mammals consuming pyraclostrobin-treated sugar beets seeds as acute risk quotients for 15-gram mammals were exceeded at the Acute Restricted Use and Acute Listed Species Use risk presumption categories (RQ=0.26)

• Potential chronic risks to I5-grammammals ingesting pyraclostrobin-treated canola seeds when applied as a seed-treatmentto rapeseed as chronic risk quotients were exceeded (RQ=1.72)

3

### • Potential chronic risks to I5-gram mammals ingesting pyraclostrobin-treated sugar beet seeds as chronic risk quotients for I5-grammammals were exceeded at the chronic levels ofconcern (RQ=22.93)

• Potential acute risk to freshwater(cold-water) fishes from use ofpyraclostrobin as a seed treatmentto canolaas acute risk quotients are exceeded at the Acute Listed Species risk presumption category (RQ=O.061)

• Potential acute risks to salt-waterinvertebrates (mysid shrimps) from use of pyraclostrobin as a seed-treatmentto canolaas acute risk quotients are exceeded at the Acute Listed Species risk presumption category (RQ=O.09)

• Potential acute risk to freshwater fishes (Warm-waterBluegill sunfish and the cold-waterRainbow trout fishes) from the use ofpyraclostrobin as an encapsulated formulation on com (field, pop, and seed production) as acute risk quotients were exceeded at the Acute Restricted Use and Acute Listed Species Use (rainbowtrout) and at the Acute Listed Species Use (bluegill sunfishes) risk presumption categories (Rainbow trout Acute RQ=O.II and Bluegill sunfish Acute RQ=O.06)

• Potential acute risk to salt-watermollusks(eastern oysters) from the use of pyraclostrobin as an encapsulated formulation on com (field, pop, and seed production) as levels ofconcern were exceeded at the Acute Listed Species risk presumption category (Eastern OysterAcute RQ=O.054)

• Potential acute risks to salt-waterinvertebrates (mysid shrimp) from the use of pyraclostrobin as an encapsulated formulation on com (field, pop, and seed production) as levels ofconcern were exceeded at the Acute Restricted Use and Acute Listed Species use risk presumption categories (Mysid shrimp Acute RQ=O.16)

• Potential acute risks to freshwater fishes (rainbow trout) from the use of pyraclostrobin as a seed-treatmentto sugar beet seeds as acute levels ofconcern were exceeded at the Acute Listed Species Use risk presumption. Also, acute levels ofconcern were triggered at the Acute Listed Species Use risk presumption category for warm-water fishes (bluegill sunfishes) and at the Acute Restricted Use risk presumption category for cold-waterfishes (rainbowtrout) (Rainbow trout acute RQ=O.IO and Bluegull sunfish Acute RQ=O.05)

• Potential acute risks to salt-water invertebrates (mysid shrimp) from the use of pyraclostrobin as a seed-treatmentto sugar beets as acute levels ofconcern were exceeded at the Acute RestrictedUse and Acute Listed Species Use risk presumption categories (Acute RQ=O.13)

4

### • No potential acute and/or chronic risks to avian and mammalian organisms ingesting pyraclostrobin-treated sweet com seeds as acute and chronic risk quotients were all less than 0.01 in value.
• No potential risks to terrestrial and/or aquatic vascularand non-vascularplants from the use ofpyraclostrobin as a seed-treatmentto the seeds ofsweet com, sugar-beets, and rapeseeds as risk quotients were belowthe levels ofconcern as risk quotients ranged from <0.01-0.13 for aquatic plants and risk quotients for terrestrial plants were all less than 0.1.
• No potential acute and/or chronic risk to aquatic organisms and amphibians from the use ofpyraclostrobin as a seed-treatmentto the seeds ofsweet com, sugarbeets, and rapeseeds as risk quotients were <0.01.
• No potential risk to non-targetterrestrial insects from the use ofpyraclostrobin as a seed-treatmentto the seeds ofsweet com, sugar-beets, and rapeseeds and as an encapsulationproductto com (field, pop, and seed-production).


### Data Gaps and Uncertainties:
1. Guideline aquatic metabolism studies in soil have notbeen submitted. Since the registrant did not providethe Agency with the appropriate guideline studies for anaerobic aquatic metabolism (835.4400) and aerobic aquatic metabolism (835.4300) anaerobic soil metabolism and aerobic soil metabolism data was extrapolated for these studies following the "Guidance for Selecting Input Parameters in Modeling the Environmental Fate and TransportofPesticides; Version 2.1, October22,2009. Aerobic aquatic metabolism and anaerobic aquatic metabolism data will provide a basis for a more refined aquatic exposure assessment for future new uses.


### 2. Guideline estuarine/marine invertebrate lifecycle/chronic reproductive study exposing pyraclostrobin to estuarine/marine invertebrates on a chronic reproductive basis (850.1350) have notbeen submitted. There is insufficient data to definitively characterize chronic risks to estuarine/marine invertebrates. However, acute toxicity data suggestthat estuarine/marine invertebrates (mysids) are more sensitive to pyraclostrobintoxicitythan freshwater invertebrates. For example, the acute LC

### sovalues for the mysid shrimp 

### (Americamysis bahia) and daphnids 

### (Daphnia magna) are 4 ppb (MRID 45118412) and 16 ppb (MRID 45118407), respectively. Furthermore, the relationship between acute and chronic endpoints for freshwater invertebrates, when appliedto estuarine/marine acute data, suggests apossible chronic concern for estuarine/marine invertebrates. Because ofthis relationship and the fact that several crop uses of pyraclostrobin are associated with estuarine/marine habitat, a life-cycle test for mysids (850.1350) must be submitted.

5

### 3. 

### Whole Sediment acute toxicity invertebrates (freshwater)**test~850.1735** 

### have not been submitted. In the aquatic ecosystems systems, pyraclostrobin is likely partitionedto sediments. Thus, sedimenttoxicity data for aquatic invertebrates are needed in orderto assess the risks to sediment dwelling organisms. Since pyraclostrobin may potentially bind to sediment and it is also found to be characterized on an acute basis as "very highly toxic" to freshwater invertebrates inhabiting the water column, this study mustbe submitted. Moreover, the lack ofthe study will be considered as a "Data Gap" until the Agency receives this study.
4. 

### Whole sediment acute toxicity invertebrates (marine) test-850.1740 have not been submitted. As stated earlier in this assessment, pyraclostrobin may potentially bind to sediment in aquatic environments. Since pyraclostrobin may potentially bind to sediment and it is also found to be characterized on an acute basis as "very highly toxic" to salt-water invertebrates inhabiting the water column, this study mustbe submitted. Moreover, the lack ofthe study will be considered as a "DataGap" until the Agency receives this study 5. Ecotoxicological, toxicological and environmental fate data for the three major degradates formed from the breakdown ofthe parentpyraclostrobin, BF 500-11, BF 500-13, and BF 500-14, were only submitted for freshwater fishes and invertebrates. No other info on the environmental fate, ecotoxicity, and toxicity effects these degradates may exert on other aquatic and terrestrial organisms was submitted for use in this assessment. Therefore, the environmental fate ofthese degradates in the environment as well as overall the ecotoxicity and toxicity effects these degradates may pose terrestrial organisms (either directly or indirectly) are unknown at this time.
6. The use ofthe acute to chronic ratio to estimate a No Observable Adverse Effect Concentration (NOAEC) value for estuarine/marine invertebrates. This method usually introduces a high level ofuncertainty into the overall risk estimation and risk conclusions as the risks may have been over or under-estimated.
7. This assessment does not analyses otherpossible routes ofexposure such as inhalation and accidental ingestion ofsoil sediments to which pyraclostrobin couldbind in terms ofterrestrial and aquatic organisms being exposed. 

### 2. Scope of This Assessment
An abbreviatedreview was conductedby EFED for the following reasons.
• No new datahave been submittedthat affectthe environmental fate or effects conclusions or modeling inputs;

• Proposed application rates, intervals, and seasonal loads are less than previously approved application rates;

6

### • No new exposure methods have been approved for standardrisk assessments since the last chemical assessmentwas performed in 2010. 

### 3. Findings of Prior Risk Assessments
Previous pyraclostrobinrisk assessments by EFED indicate the following direct and indirect effects in terrestrial and aquatic organisms:

• Mammals and aquatic organisms 

### are subjectto adverse direct effects such as reduced survival, growth, and reproduction when exposed to pyraclostrobinresidues as a result oflabeled use ofthe pesticide. Birds 

### are not subjectto adverse direct effects such as reduced survival, growth, and reproduction when exposedto pyraclostrobin residues as a result oflabeled use ofthe pesticide.

• Non-targetterrestrial, semi-aquatic, and aquatic plants 

### are not subjectto adverse effects such as reductions in vegetative vigor and seedling emergence (terrestrial) or biomass and growthrate (aquatic) when exposedto pyraclostrobinresidues as a result oflabeled use ofthe pesticide.

• Indirect effects, such as food web dynamics, perturbing forage or prey availability, and altering the extent and nature ofnesting, 

### will potentially occur as residue concentrations exceeded levels ofconcern for acute or chronic exposure forterrestrial and/or aquatic species. • Listed species 

### are subjectto adverse effects as the calculatedrisk quotients exceeded Acute Listed or Chronic Levels ofConcern. 

### 4. Environmental Fate Summary Pyraclostrobin (cabamic acid, [2-[[[1-(4-chlorophenyl)-1H-pyrazol-3- yl[oxy]methyljphenyl]methoxy-,methyl ester) belongs to the strobilurin family of fungicides. The mode ofaction ofpyraclostrobin is believedto be the inhibition of mitochondrial respiration in fungal cells. Pyraclostrobin, like other strobilurin fungicides, also inhibits spore germination and is reported as apowerful antisporulant. Although strobilurins do not have systemic activity, they exhibitunique translaminar activity, i.e., the fungicide could move from the sprayed side to the unsprayed side ofa given leaf. In this context, they are classified as 'surface systemic' or 'mesosystemic' fungicides. 

### Figure 1 displays the molecular structure ofpyraclostrobin. 

# (')**Cr-Q-N(YO~**
- - /0I(N CH3 

### H C ' "

3 

## 0 

# °

7

### Figure 1: Molecular Structure of Pyraclostrobin
A majorroute ofdissipation for pyraclostrobin is runoff. Two main factors contribute towards pyraclostrobin ability to contaminate surface watervia runoff. First, pyraclostrobin is practically immobile with Kocs between 6,000 mg/L and 16,000 mg/L in all soil studies with an arithmetic mean of9,304 mg/L and median of7,889 mg/L, Second, laboratory studies suggestthat pyraclostrobin is moderately persistentin aerobic soil environments (t1/2

## =81.5 days). Adsorption/desorption studies suggestthat pyraclostrobinhas a tendency to adsorb to the soil particles. Laboratory aerobic soil metabolism studies are supportedby field dissipation studies findings suggesting that pyraclostrobin is moderately persistentin soil. Aqueous photolysis (T1/2 = 

### 0.08 days) and anaerobic soil metabolism (t1/2 = 

### 3.0 days) appearto be the mainroutes ofdegradation. Pyraclostrobin is consideredto be stable to hydrolysis and soil photolysis. Fish bioaccumulation data suggest, Log Kow 4.18 at pH 6.5 thatpyraclostrobinhas atendency to bioaccumulate in fish; however, depuration is rapid. 

### 5. Summary of Proposed Uses The proposed new use for Coronet (9.0% a.i.) seed treatmenton rapeseed allows for a maximum application of0.12 lbs a.i. per 100 lbs. ofseed. The maximumrapeseed (cultivars, varieties, and/orhybrids, including canola and crambe) seeding rate is 8 lbs of seed per acre (http://www.ipmcenters.org/cropprofiles/CP form.cfm); therefore, the maximum application rate for rapeseed is 0.0096 lbs. a.i.lA. 

### The new uses and proposed application rates and methods are listed in 

### Table 5.
The proposed increased use rate for Stamina (18.4% ai) seed treatment on sweet com permits a maximum application of0.02 lbs. a.i. per 100 lbs ofseed. The maximum seeding rate for sweet com is 15 lbs a.i.lA (http://nwrec.hort.oregonstate.edulcornfr.html); therefore, the maximum applicationrate for Staminaon sweetcom is 0.003 lbs. a.i.lacre.
The proposed decreased use rate for Staminaon sugarbeetpermits a maximum of0.033 lbs. a.i. per 100,000 seeds (40,000 seeds per pound, http://sugarbeet.ucdaviseduinoteslJune98.html) at a maximum seeding rate of8 lbs. (http://www.ageconucdavis.eduioutreachicrop/Archives/SugarBeets.pdf) peracre, therefore, the maximum applicationrate for Staminaon sugarbeet is 0.1056 lbs. a.i.lacre.
The maximum applicationrate for BAS 50020 F (23.06 %) is one application ofO.llbs. a.i.lA per year. BAS 500 20 F is a new encapsulatedproduct comparable in its proposed use pattern and percentage active ingredientto Headline® Fungicide (23.6 a.i.lA)(EPA Reg. No. 7969- 186). BASF submitted "The Wash-offofEncapsulated Pyraclostrobin (BAS 500 F) from Com Plants" non-guideline study (MRID # 48037313 ) for the registration ofBAS 500 20 F Fungicide to address the dissipation/release rate ofthe active ingredient from the wash-offofencapsulated material that will be deposited onto soil. This study was reviewed and classified ancillary information.



### 8

### BASF also submitted "Comparison ofPyraclostrobin (BAS 500 F) Field Soil Dissipation Endpoints from North Americawith EC, WG, and CS Formulations" paper (MRID # 48037314) to demonstrate that formulation type is not an important factor influencing the field dissipation ofpyraclostrobin. This study was reviewed and classified ancillary information. 

## Table 5. Pyraclostrobin Application Rates for BAS 500 20 F, Coronetand Stamina Formulations Formulation Crops Max. Max. #of Type of Application Rate Applications Application

lbs. a.i./Acre BAS50020 F Field com, pop com and seed 0.10 I Foliar [production com (Aerial/Ground) 

### Coronet Rapeseed (cultivars, varieties, and/orhybrids, including 0.0096 1 Seed treatment canola and crambe) 

### Stamina Sweet com 0.0034 1 Seed treatment Sugarbeet 0.1056 1 Seedtreatment 

## TableAt in 

## AppendixA shows a listing ofcrops already registered for pyraclostrobin use. 

## 6. Exposure Summary 

## 6.1 TerrestrialExposures Consumption ofpyraclostrobin on contaminated foliage, soil or seeds are the most likely exposure route for terrestrial animals. The Terrestrial Exposure (TREX) Model (Version 1.4.1) was used to estimate the dietary exposure to terrestrial birds and mammals. Typical input values (for foliar treatments) for TREX include applicationrate, time intervals between applications, total number ofapplications, foliarhalf-life periods, and toxicity data. TREX modeled inputparameters and outputs are summarized in 

## Appendix E. Bird and Mammal Exposure Assessment Estimated exposure concentrations for terrestrial receptors were determined using the standard screening-level exposure model, TREX (v. 1.4.1.) (EPA, December7,2008). Essentially, for a single application, there is a linearrelationship betweenthe amount of pesticide applied and the amount ofpesticide residue present on a given food item. These relationships for the various food items are determined from the Kenaganomogram as modifiedby Fletcher (Hoerger and Kenaga, 1972; Fletcheret aI., 1994). TREX (v.1.4.1) is a terrestrial exposure model that, in addition to incorporating the nomogram relationship, also includes pesticide degradation in the estimate ofEECs.
Foliardissipation half-lives are incorporated into the TREX model. The foliar dissipation half-life default of35 days was used to provide an upper-boundpyraclostrobin residue concentration on foliage due to no foliar residue dissipation data being available from the



### 9

### Agency's HumanEffect Division (HED). Maximum Kenaga dose-based and dietary EECs for a single encapsulated spray application (aerial and/or ground) ofpyraclostrobin (BAS 500 20F) to comusing the recommended label rate for birds are shown in 6 and 7 while the maximum Kenaga dose-based EECs for mammals is presented in 

## Tables 8 

## and 9. EECs for seed treatmentuses are presented in 

## Table 10.



## Table 6. Estimates of Avian Dietary Based-maximum KenagaEECs for FoliarResidues I 

## ofPyraclostrobin on Corn (Field, Pop, and Seed-production) with a FoliarDissipation tl/2= 

## 35 day Half-life Value and a SingleApplication UsefApp. Application Rate Ibs. a.i.fA Food Items MaximumEEC Method (# app finterval, days) (mglkg) Com (field, pop, Short grass 24,00


### I and seedproduction) 0,10 Ibs ai/A with only I Tall grass 11.00 (Encapsulated application per growing season Spray Aerial per year Broadleafplants/small insects 13,5

- and/or ground

I Fruits, pods, seeds, and large insects 1.50 applications) I ....-J 

## Table 7. Estimates ofAvian Dose-Based Maximum KenagaEECs for use ofDAS 500 20F on Com (Field, Pop, and Seed-production) with a FoliarDissipationtl/2= 

## 35 Day Half-life Value and a SingleApplication UsefApp. Application Food Items 20 grams 10o-grams lOOO-grams Method Rate Ibs.a.iJA (# app finterval, davsf Com (field, pop, 0,10 Ibs aifA with Short Grass 27.33 15,59 6,98 and seed- only I production) application per (Encapsulated growing season Spray Aerial per year and/or ground applications) Tall grass 12,53 7,14 320 Broadleafplants/small insects 15,38 8,77 3,93 Fruits/pods/seeds/large insects 1.71 0,97 0.44
Granivores 0.38 0.22 0,10


## Table8. 

## Es"'tes of MI

...... 

## 588.F

# o. €onl(l'iet8,

. 

## 1-l

.....iIId 

## HaIf.. Iife"VaIue**aada,Single~'** Usd4pp. Method
Com (field, pop,and seedproduction) (Encapsulated Spray Aerial and/or ground a lications)

## ,'~" .Rate**ibi~a.iJA** (# app IiatervaI, 

### dlts 0, I0 Ibs ailA with Short Grass only I application per growing season per year



### 10 15gi

__

22,88 15,81 367Tall grass 10.49 7.25 1.68 Broadleafplants/small insects 12.87 8.90 2.06 Fruits/pods/seeds/large insects 1.43 0.99 0.23 Granivores 0.32 0.22 0.05


## Table 9. Estimates of Mammalian Dietary Based-maximum KenagaEECs for Foliar Residues ofPyraclostrobin on Corn (field, pop, and seed-production) with a Foliar Dissipation till

# =35 Days Half-life Value and a Single Application UseiApp. Application Rate Ibs. a.iJA Food Items MaximumEEC Method (# app/ interval, days) (mg/kg) Corn (field, pop, Short grass 24.00 and seed- 0.10 Ibs ai/A with only I production) Tall grass 11.00 application per growing season (Encapsulated per year Broadleafplants/small insects 13.5 Spray Aerial and/or ground Fruits, pods, seeds, and large insects 1.50 applications) 

### For seed-treatment, T-REX seed-treatmentmodel (v.1.4.1) was used to estimate avian and mammalianEECs for terrestrial organisms exposedto pyraclostrobin-treated sweetcorn seeds. These EECs are presented in 

## Table 10 ofthis document. The EECs are expressed as Exposed avianlNAGY Dose (mg ai/kg-bw/day) and marnmalianlNAGYDose (mg ai/kg-bw/day) for the soil incorporation ofsweet corn, , canola seeds, and sugarbeet seeds 

## Table 10. Seed TreatmentEECs for Pyraclostrobin Applied As a Seed Treatment for Sweet Corn, Canola and SU2arBeet Seeds Maximum Maximum Seed Avian Nagy Dose Mammalian Nagy Available ai Applicationrate Application Rate (mg ai/kg-bw/day) (mg ai/kg-bw/day) (mg ai ft -2) (lbs ai/a) (mg ai/kg seed)

Sweet com 0.003 200.00 50.62 42.37 0.03

Sugarbeet 0.11 13,300.00 3366.08 2817.90 1.11 Rapeseed 0.01 1000.00 253.09 211.87 0.08 

## 5.2 AquaticExposures 5.2.1 Input Parameters
The maximum applicationrates for Coronet, BAS 50020 F and Staminainput into PRZMlEXAMS are listed in 

## Table 5 on page 9. 

### PRZM/EXAMS input parameters for pyraclostrobin are shown in 

## AppendixB and example PRZMlEXAM outputfiles are displayed in 

## Appendix C.
5.2.2 AquaticExposure Results The MS corn scenario generatedthe highestpeak. (0.68 ug/L) and 60 day EEC 

### (G.Ilug/L) and the NC corn ground scenario produced the highest 21-day (0.19 ug/L) averages. Results ofthe 

### PRZM/EXAMS modeling are displayed in 

## Table 11.

11

### Table 11. Estimated Environmental Concentrations ofPyraclostrobin Crop Scenarios Peak 21day Cone. 60 day Cone. Cone.(uz/L) (uz/L) (ul!!L) 

### Coronet (seed treatment) (0.012 1bs. a.i./acre) ND Canola 0.38 0.11 0.06 BAS 500 20 F (l 

### aerial application at O.llbs ai/acre) MSCorn 0.68 0.18 0.11 NCCorn 0.60 0.19 0.09 ILCorn 0.39 0.17 0.10 Stamina (seed treatment) Corn (0.003 lbs. a.i./acre) MS Sweet Com 0.02 0.007 0.003 NC Sweet Com 0.005 0.001 0.0008 IL Sweet Com 0.01 0.004 0.002 SU2ar Beet (0.104Ibs. a.i./acre) MN Sugar Beet 0.56 0.18 0.06 

### 5. Ecological Effects Summary
Based on the aquatic toxicity studies, the toxicity endpoints used for the aquatic risk assessment ofpyraclostrobin are characterized as follows.



## •

•

•

•

•

•
•



## •

•

•

• Fish (freshwater) acute: Very highly toxic (LCso = 6.2 ppb) Fish (freshwater) chronic: (NOAEC = 2.35 ppb) Fish (estuarine/marine) acute: Very highly toxic (LCso= 

### 76.9 ppb) Fish (estuarine/marine) chronic: (NOAEC = 

### 10.8 ppb) Invertebrate (freshwater) acute: Very highly toxic (ECso = 15.7 ppb) Invertebrate (freshwater) chronic: (NOAEC = 4.0 ppb) Invertebrate (estuarine/marine) acute: Very highly toxic (LCso= 

### 4.16 ppb) Invertebrate (estuarine/marine) chronic: No dataACRused to estimate NOAEC Vascular plants (freshwater, Non-endangered) acute: (ECso= 1720 ppb) Vascularplants (freshwater, Endangered) acute: (NOAEC 

## = 896 ppb) Non Vascular Plants (freshwater) acute: (ECso= 1.5 ppb) Non VascularPlants (estuarine/marine) acute: (ECso = 

### 64.7 ppb) Based on the terrestrial toxicity studies, the toxicity endpoints used for the terrestrial risk assessment ofpyraclostrobin are characterized as follows.



## •
•
• 

### Avian acute oral: Practically non-toxic (LDso : >2000 mg a.i.lKg BW/day) Avian acute dietary: Practically non-toxic (LCso : >5000 mg a.i.lKg diet or PPM) Avian reproduction: (None ofthe endpoints affectedup to the highest dose tested; NOAEC: 1062 mg a.i.lKg diet or PPM)

12

### • Mammalian acute oral:(LDso: >5000 mg a.i.lKg BW for males and females)

• Mammalian chronic reproduction: (Prenatal developmental toxicity study in rabbits; fetal resorptions and post implantation loss, NOAEL: 5 mg a.i.lKg BW/day)

• Mammalian chronic reproduction(NOAEL= up to 300 mg/kg-diet in mammals)

• Honey bee acute: Practically nontoxic (LDso:>100 ug a.i./bee)

• Seedling Emergence, Tier I (Monocots and Dicots) (EC2s 21 days (Monocots and Dicots): > 0.118 Ib a.i. /acre (percentemergence, percentsurvival, phytotoxicity rating, plant heightand dry weight)NOAEC : 0.118 Ib a.i. /acre

• Vegetative Vigor Tier I (Monocots and Dicots) EC2s 21 days (Monocots and Dicots): > 0.1181b a.i. /acre (Same parameters as in Emergence) NOAEC : 0.118 Ib a.i. /acre • (Same parameters as in Emergence) Full listing ofthe toxicity and ecotoxicity data for pyraclostrobin exposure to terrestrial and aquatic organisms and plants is available in

## Appendix G ofthis assessment. 

## 6. Ecological Risks Summary
A full summary ofthe potential risks identified in this assessment is presented in the subsequent sections. A complete list of aquatic organismrisk quotients (acute and chronic) are presented in 

## Appendix D. 

## Tables 11-14 show the acute and chronic risk quotients ofthe most sensitive aquatic organisms for exceedence ofthe LOC did occur: 

## Table 11. Aquatic OrganismRiskQuotients ofSTAMINA to SugarBeets Seeds (O.104Ibs. a.iJacre) Aquatic Acute Chronic Peak 21- 60- Acut 

### Chroni LOC Organisms Toxicit Toxicity EEC day Day eRQ cRQ Exceedenc yValue Value (flg/L 

### EEC EEC e (flg/L) (flg/L) ) 

### {flfL (flg/L ) 

### Rainbow Trout LCso

### 96 N/A 0.56 0.18 0.06 0.10 Acute (Oncorhynchu hr: 6.2 Restricted s mykiss- Use LOC is Warm-water triggered fish) and Acute Listed Species Use is exceeded Fathead N/A NOAEC=4.14 0.56 0.18 0.06 0.01 None Minnow (Pimephales promelasy 

### Rainbow Trout N/A NOAEC=2.35 0.56 0.18 0.06 0.03 None (Oncorhynchu s mykiss) Sheepshead LCso

### 96 NOAEC=10.8 0.56 0.18 0.06 <0.01 <0.01 None Minnow hr: 76.9 (Cyprinodonvariegates

### 13Estuanne/manne rnysid Chronic NOAEC Value was estimated usmg the ACR method and the value may be over or underestimated. 

### Salt-Water Fish) Daphnia ECso

### 48 0.56 0.18 0.06 0.04 None magna hr: 15.7 a.i. (14.2- 17.4) ug/L 

### Daphnia NOAEC: 41lg 0.56 0.18 0.06 0.05 None magna a.i. /L
Eastern Oyster ECso

### 96 0.56 0.18 0.06 0.04 None hr: 12.5 (Crassostrea a.i.(9.2- virginica) 17.1) Mollusk Shell Ilg/ L Deposition Mysid Shrimp 

## r.c., 96 0.56 0.18 0.06 

### 0.13 Acute hr:4.16 Restricted (Americamysis Use and bahia) Acute Listed Species LOCs are exceeded Mysid Shrimp NOAEC=I.06 0.56 0.18 0.06 0.17 None (Americamysis I 

### bahia)

1.



### Table 12. Aquatic Organism Risk Quotients ofBAS SOO 20 F (1 Application at 0.1 Ibs. a.i.lA Applied to Com (Field, Pop, and Seed Production) Aquatic Acute ChronicToxicity Peak 21-day 6O-Day Acute Chronic LOC Organisms Toxicity

. Value EEC EEC EEC RQ RQ Exeeedenee Value (11g!L) (11g!L) (11g!L) (11g!L) (11g!L) 

### Rainbow Trout LCso

### 96 N/A 0.68 0.18 0.11 0.11 

### Yes (Oncorhynchus hr: 6.2 Acute mykiss-Warm- Restricted water fish) Use and Acute Listed Species OC exceeded Fathead N/A NOAEC=4.14 0.68 0.18 0.11 0.03 None Minnow (Pimephales promelas) Rainbow Trout N/A NOAEC=2.35 0.68 0.18 0.11 0.05 None (Oncorhynchus mykiss)



### 14Estuarme/marme mysid Chrome NOAEC Value was estimated usmg the ACR method and the value may be over or underestimated. Sheepshead LCso96 NOAEC=10.8 0.68 0.18 0.11 <0.01 0.01 None Minnow hr: 76.9 

### (CyprinodonvariegatesSalt-Water Fish) 

### Daphnia ECs o48 0.68 0.18 0.11 0.043 None 

### magna hr: 15.7 a.i. (14.2- 17.4) flg/L 

### Daphnia NOAEC: 4 ug 0.68 0.18 0.11 0.05 None 

### magna a.i. /L

Eastern Oyster ECso96 0.68 0.18 0.11 0.054 Acute hr: 12.5 Listed 

### (Crassostrea a.i.(9.2- Species 

### virginica) Mollusk Shell 17.1) LOC Deposition flg/L exceeded Mysid Shrimp LCso96 0.68 0.18 0.11 0.20 Acute hr:4.16 Restricted 

### (Americamysis Use and 

### bahia) Acute Listed Species LOCs are exceeded Mysid Shrimp NOAEC=I.06 0.68 0.18 0.11 0.20 None 

### (Americamysis bahia)

I.



## Table 13-Coronet(seed treatment to Canolaand CrambeO.0121bs ai/acre) and Aquatic RiskQuotients Aquatic Acute Chronic Peak 21- 

### 60- 

### Acut Chroni LOC Organisms Toxicity Toxicity EEC day Day eRQ cRQ Exceedenc Value Value (flg/L 

### EEC EEC e (flg/L) (flg/L) ) (ug/L (ug/l, ) ) Bluegill LCso96 N/A 0.38 0.11 0.06 0.03 N/A None Sunfish hr: 11.4 

### (LepomismacrochirusColdwater fish) Rainbow Trout 

## r.c., 96 N/A 0.38 0.11 0.06 

### 0.061 Yes Listed 

### (Oncorhynchu hr: 6.2 Species OC 

### s mykiss- exceeded Warm-water



### 15fish) Fathead N/A NOAEC=4.14 0.38 0.11 0.06 0.01 None Minnow 

### (Pimephales promelas) Rainbow Trout N/A NOAEC=2.35 0.38 0.11 0.06 0.03 None 

### (Oncorhynchu s 

### mykiss) Sheepshead LCso96 NOAEC=10.8 0.38 0.11 0.06 <0.01 <0.01 None Minnow hr: 76.9 

### (CyprinodonvariegatesSalt-Water Fish) 

### Daphnia ECso48 0.38 0.11 0.06 0.024 NO 

### magna hr : 15.7 a.i. (14.2- 17.4) llg/L 

### Daphnia NOAEC: 411g 0.38 0.11 0.06 0.028 None 

### magna a.i. IL

Eastern Oyster ECso96 0.38 0.11 0.06 0.034 None hr: 12.5 

### (Crassostrea a.i.(9.2- 

### virginica) 17.1) Mollusk Shell Deposition llg/L Mysid Shrimp 

### t.c., 96 0.38 0.11 0.06 

### 0.09 Yes Acute hr: 4.16 Listed 

### (Americamysis Species Use 

### bahia) LOCs are exceeded Mysid Shrimp NOAEC=1.06 0.38 0.11 0.06 0.10 None 

### (Americamys is 1 

### bahia)



### 16

## Tablel4- Shows the Acute and Chronic RiskQuotients forTerrestrialOrganisms Consuming Seed-erops Treatedwith Pyraclostrobin. LOCs Appearin Bold:

, Crop Maximum Maximum Avian Mammalian Available Avian Avian Mammalian Mammalian Application Seed Nagy Nagy(mg 

### ai 20 Chronic 15 gram 15 gram rate Application Dose ai/kg- (mg ai 

### ft gram Acute I chronic (lbs ai/a) Rate (mg bw/day) -2) Acute (mgailkg ai/kgseed) bw/dav) Sweet 0.003 200.00 50.62 42.37 0.03 0.05 0.19 <0.01 0.34 com Sugarbeet 0.11 13,300.00 3366.08 2817.90 1.11 3.241 12.523 0.262 22.93 4 Rapeseed 0.01 1000.00 253.09 211.87 0.08 0.245 0.94 0.02 1.726

Avian acute levels ofconcern are exceeded for 20-grambirds consuming sugarbeet seeds treated WIth pyraclostrobin at the Acute Risk, Acute restricted Use, and Acute Listed Species Risk presumption categories 2. Mammalian acute levels ofconcern are exceeded for IS-gram mammals consuming sugarbeet seeds treated with pyraclostrobin at the Acute Restricted Use and Acute Listed Species Risk presumption categories
3. Avian chronic levels ofconcern are exceeded for 20-gram birds consuming sugarbeet seeds treated with pyraclostrobin**(RQ~I** for exceedence to occur)
4. Mammalian chronic levels ofconcern are exceeded for IS-gram mammals consuming sugarbeet seeds treated with pyraclostrobin**(RQ~I** for exceedence to occur) 5. Avian acute levels ofconcern exceeded for 20-grambirds consuming rapeseed seeds treated with pyraclostrobin at the Acute Restricted Use and Acute Listed Species Use risk presumption categories 6. Mammalian chronic levels ofconcern are exceeded for IS-gram mammals consuming rapeseed seeds treated with pyraclostrobin**(RQ~1** for exceedence to occur) 

### Terrestrial plant risk quotients were for all crops modeled in this assessment did not triggeror exceedence any levels ofconcern. Terrestrial plant risk quotients ranged from <0.1-0.13 for all crops modeled. For more details please see Appendix F 

## 7. Endangered and Threatened Species Tables 15-18 summarizes the conclusions ofpotential concerns for direct and indirect effects to federally-listed threatened and endangered species (listed species). 

## Table 15. Listed Species Risks AssociatedWith DirectorIndirectEffects Due to Pyraclostrobin being used as an encapsulated formulation on corn (seeds, field, and pop). Listed Taxonomy Direct Effects Indirect Effects Terrestrial and semi-aquatic No Yesa plants - monocots Terrestrial and semi-aquatic No Yesa plants - dicots Terrestrial invertebrates No No Birds No Yesc Terrestrial-phase amphibians Yesb Yes c Reptiles Yesb Yesc Mammals Yes Yes" Aquatic vascularplants No No



### 17

### Table 15. Listed Species Risks Associated With Direct or IndirectEffects Due to Pyraclostrobin being used as an encapsulated formulation on corn (seeds, field, and pop). Listed Taxonomy Direct Effects Indirect Effects 

### Aquatic non-vascularplants a 

### No No Freshwaterfish Yes Yes Aquatic-phase amphibians YesD Yesc 

### Freshwater Invertebrates Yes Yesc 

### Mollusks Yes Yesc 

### Estuarine/marine Inverts Yes Yesc 

### Marine/estuarine fish Yes Yesc

a

### At the date ofthis publication, there are no listed aquatic non-vascularplant species. The taxonomic group is included here for the purposes ofevaluating potential contributions to indirect effects to other taxonomy and as a record ofexceedances should future listings ofnon-vascular aquatic plants warrant additional evaluation ofFederal actions. b 

### Terrestrial-phase amphibians and reptiles estimatedusing birds as surrogates. Aquatic-phase amphibians estimatedusing freshwater fish as surrogates.

C

### Animals that feed on aquatic organisms may be indirectly affected. d 

### Indirect effects may be caused by plants that rely on fungus for growth or reproduction or that rely on mammals as pollinators or seed dispersers.


### Table 16. 

### Listed Species Risks Associated With Direct or Indirect Effects Due to Pyraclostrobin 

### Being Used as a Seed-treatment to SU2arBeet Seeds Listed Taxonomy DirectEffects Indirect Effects 

### Terrestrial and semi-aquatic No Yesd 

### plants - monocots Terrestrial and semi-aquatic No Yeso 

### plants - dicots Terrestrial invertebrates No Yes Birds Yes Yesc 

### Terrestrial-phase amphibians YesD Yes c 

### Reptiles NoD Yesc 

### Mammals Yes Yesc 

### Aquatic vascularplants No No Aquatic non-vascularplants a 

### No No Freshwaterfish Yes Yes Aquatic-phase amphibians Yes Yesc 

### Freshwater Invertebrates No Yesc 

### Mollusks No Yesc 

### Estuarine/marine inverts Yes Yes Marine/estuarine fish No Yesc

a 

### At the date ofthis publication, there are no listed aquatic non-vascularplant species. The taxonomic group is includedhere for the purposes ofevaluating potential contributions to indirect effects to other taxonomy and as a record ofexceedances should future listings ofnon-vascular aquatic plants warrant additional evaluation of Federal actions. b 

### Terrestrial-phase amphibians and reptiles estimated using birds as surrogates. Aquatic-phase amphibians estimatedusing freshwater fish as surrogates.

C 

### Animals that feed on aquatic organisms may be indirectly affected. d 

### Indirect effects may be causedby plants that rely on fungus for growth or reproduction or that rely on mammals as pollinators or seed dispersers.



### 18

### Table 17. Listed Species Risks Associated With Direct or IndirectEffects Due to Pyraclostrobin Beine:Used As a Seed-treatmentto Sweet Corn Seeds Listed Taxonomy Direct Effects Indirect Effects Terrestrial and semi-aquatic No YesG plants - monocots Terrestrial and semi-aquatic No YesG plants - dicots Terrestrial invertebrates No No Birds No Yesc Terrestrial-phase amphibians Nob Yes c Reptiles Nob Yesc Mammals No Yesc Aquatic vascularplants No No Aquatic non-vascularplants a No No Freshwater fish No Yes Aquatic-phase amphibians No Yesc Freshwater Invertebrates No Yesc Mollusks No Yesc Estuarine/marine Inverts No Yesc Marine/estuarine fish No Yesc

aAt the date ofthis publication, there are no listed aquatic non-vascularplant species. The taxonomic group is includedhere for the purposes ofevaluating potential contributions to indirecteffects to other taxonomy and as arecord ofexceedances should future listings ofnon-vascular aquatic plants warrant additional evaluation ofFederal actions. b Terrestrial-phase amphibians and reptiles estimatedusing birds as surrogates. Aquatic-phase amphibians estimated using freshwater fish as surrogates.

CAnimals that feed on aquatic organisms may be indirectly affected. d Indirect effects may be caused by plants that rely on fungus for growth orreproduction or that rely on mammals as pollinators or seed dispersers. 

### Table 18. Listed Species Risks Associated With Direct or IndirectEffects Due to Pyraclostrobin BeingUsed as a Seed-treatmentto Canola seeds Listed Taxonomy Direct Effects Indirect Effects Terrestrial and semi-aquatic No YesG plants - monocots Terrestrial and semi-aquatic No YesG plants - dicots Terrestrial invertebrates No No Birds Yes Yesc Terrestrial-phase amphibians Yes Yes c Reptiles No Yesc Mammals No Yesc Aquatic vascularplants No Yesc Aquatic non-vascularplants a No Yesc Freshwater fish Yes Yes Aquatic-phase amphibians No Yesc Freshwater Invertebrates No Yesc Mollusks No Yesc Estuarine/marine inverts Yes Yesc Marine/estuarine fish No Yesc



### 19

### Table 18. Listed Species Risks Associated With Direct or IndirectEffects Due to Pyraclostrobin 

### BeingUsed as a Seed-treatmentto Canola seeds Listed Taxonomy 

## I Direct Effects 

## I Indirect Effects

a

### At the date ofthis publication, there are no listed aquatic non-vascularplant species. The taxonomic group is included here for the purposes ofevaluating potential contributions to indirect effects to other taxonomy and as a record ofexceedances should future listings ofnon-vascular aquatic plants warrant additional evaluation of Federal actions. b 

### Terrestrial-phase amphibians and reptiles estimated using birds as surrogates. Aquatic-phase amphibians estimatedusing freshwater fish as surrogates.

C

### Animals that feed on aquatic organisms may be indirectly affected. d 

### Indirect effects may be caused by plants that rely on fungus for growth orreproduction or that rely on mammals as pollinators or seed dispersers.


### LOCATES is used to identify listed species that may be located within the same county as the proposed new uses. The LOCATES runs are listed in 

### Appendix H.

20

--, .... _-'----------------

### Appendix A: Registered Uses 

### ofPyraclostrobin

21

### Pyraclostrobinis presently registered for the following uses: • BAS 500ST ® 

### Seed Treatment Fungicide (RegistrationNo. 7969-240 issued May 25,2007) -Barley (ground and aerial applications) -Brassicavegetables (ground and aerial applications) -Bulb vegetables (ground and aerial applications) -Com (field, pop, sweet, and seed) (ground and aerial applications) -Curcurbits (ground and aerial applications) -Legume veges (ground and aerial applications) -Peanuts (ground and aerial applications) -Rye (ground and aerial applications) -Soybean (ground and aerial applications) -Sugar-beets (ground and aerial applications) -Sunflower (ground and aerial applications)

-Wheat (ground and aerial applications)
• BAS 516ST ® 

### Seed TreatmentFungicide (RegistrationNo. 7969-248 issued May 30, 2007) -Brassicavegetables (ground and aerial applications) -Bulb vegetables (ground and aerial applications) -Curcurbitvegetables (ground and aerial applications) -Legume vegetables (ground and aerial applications) -Soybean (ground and aerial applications) -Sunflower (ground and aerial applications)
• Cabrio EG ® 

### Fungicide (RegistrationNo. 7969-187 issued June 5, 2004) -Berries (ground and aerial applications) -Brassicahead and stem crops (ground and aerial applications) -Brassica crops (ground and aerial applications) -Bulb vegestables (ground and aerial applications) -Curcurbit vegetables (ground and aerial applications) -Fruiting vegetables (ground and aerial applications) -Grapes (ground and aerial applications) -Hops (ground and aerial applications) -Leafy vegetables (ground and aerial applications) -Leaves ofroots and tubors (ground and aerial applications)

22

### Cabrio EG ® 

### Fungicide (continued) (RegistrationNo. 7969-187 issued June 5, 2004)

-Pistaccio (ground and aerial applications) -Pomefruit (ground and aerial applications) -Root vegetables (ground and aerial applications) -Stone fruits (ground and aerial applications) -Strawberries (ground and aerial applications) -Tree nuts (ground and aerial applications)
• Cabrio Team ® 

### Fungicide (RegistrationNo. 7969-229 issued July 5, 2005) -Bulb vegetables (ground and aerial applications) -Curcurbitvegetables (ground and aerial applications) -Fruiting vegetables (ground and aerial applications)

• Headline ® 

### Fungicide (RegistrationNo. 7969-186 issued April 4, 2007)

-Barley (ground and aerial applications) -Citrus crops (ground and aerial applications) -Corn (ground and aerial applications) -Dried-shelledpeas and beans (ground and aerial applications) -Edible podded legume vegetables (ground and aerial applications) -Seed grass (ground and aerial applications) -Mint (ground and aerial applications) -Peanut (ground and aerial applications) -Pecan (ground and aerial applications) -Rye (ground and aerial applications) -Soybean (ground and aerial applications)

• Headline ® 

### Fungicide (RegistrationNo. 7969-186 issued November24,2009) -Alfalfa (ground and aerial applications)

• Headline ® 

### Fungicide (RegistrationNo. 7969-186 issued March 6, 2009) -Sorghum (ground and aerial applications)

• Headline ® 

### Fungicide (RegistrationNo. 7969-186 issued April 17, 2009) -dry beans, peanuts, and sunflower crops (in-furrow)

• Honor ® 

### Fungicide (RegistrationNo. 7969-248 issued May 30, 2007) -Golfcourse turf grass (ground applications only)

• Insignia ® 

### Fungicide (RegistrationNo. 7969-184 issued May 15,2007) -GolfCourse Turfgrass (ground applications only) -Ornamentals (ground applications only)

• Pristine ® 

### Fungicide (RegistrationNo. 7969-199 issued April 26, 2006) -Berries (ground and aerial applications) -Bulb vegetables (ground and aerial applications)

23

### -Curcurbitvegetables (ground and aerial applications) -Soybeans (ground and aerial applications) -Spinach (ground and aerial applications) -Stone fruits (ground and aerial applications) -Strawberries (ground and aerial applications) -Tree nuts (ground and aerial applications) -Almonds (ground and aerial applications)

• Stamina ® 

### Seed Treatment Fungicide (RegistrationNo. 7969-266 issued January 23, 2008) -Barley -Brassicavegetables -Bulb vegetables -Com (field, pop, sweet, com for seed, production -Cucurbits - Legume vegetables (dried shelledpeas and beans) -Peanut -Rye -Soybean - Sugar beet -Sunflower -Wheat

• Stamina ® 

### Seed Treatment Fungicide (RegistrationNo. 7969-266 issued March 6,2009) -Sorghum

• Stamina ® 

### Seed Treatment Fungicide (RegistrationNo. 7969-266 issued November, 24, 2009) -Alfalfa

24

### Appendix B: InputParameters Used in PRZMIEXAMS Modeling

25

### PRZMlEXAMS Input Parameters for Proposed Pyraclostrobin Uses I Parameter Input Value and Unit Comment Source Chemical Application Method 2 Foliar Application !GuidanCe

- (CAM) 4 Seed treatment 

## oem. canola' 

### I Planting Depth 3.81 ern. sweetcom , 0.635 em, sugarbeet 

# IMRID 45118627 I Hydrolysis (t1/2) 

## odays stable Spray drift and application Drfit- Efficiency

- Aerial: 0.05 Aerial: 0.95 iGuidance efficiency Seed Treatment: 0.0 Seed Treatment: 1.00 1248.14 days Upper 90th percentile of MRlD 45118631 Aerobic soil metabolism (tI/2)
I the mean ofhalf-lives in MRlD 45118632 I six studies MRlD 45367504 496.28 days No acceptable study No Acceptable , Isubmitted and the studies. EFED Aerobic aquatic metabolism (tI/ 2) pesticide shows IGuidance,

.insignificant hydrolysis;

, 2 times the aerobic soil half-life input 

### i [Anaerobic aquatic metabolism 6.6 days 2 times the half-life (3.3 No Studies days) ofthe anaerobic submitted ' (t1/2) [soil metabolism. 0.08 days IMaximum dark 

# IEFED Guidan,,; I Aquatic photolysis (tI/2) [controlled aqueous MRlD 45118629 , photolysis half-life of

I two studies Vaporpressure 1.95E-8 Product Chemistry Henry's Law constant (20°C) 4.13 x lO-Yatm'm'/mol Product Chemistry Solubility in water (pH 7, 20°C) 2.41 Product Chemistry Molecularweight 388 glmole IProduct Chemistry 7,889 (Germany sand) 

# I .160,000 (Germany loamy sand) i 7,889 (Germany sandy loam)

19,304 6,000 (USA loamy sand) 45160502,45160503, 

# ]Koc 9,000 (USA sandy loam) 45118703,45118701, 9,436 (Canada sandy loam) 45118702

I 9,304 (mean Koc of six soil I I Istudies) I , 1Typical agricultural practice for rapeseed is to apply the seeds to the surface ofthe soil, followed by a

process of"chaining in" the seeds 

### (e.g., pulling chains behind a vehicle to incorporate the seeds) (OMAFRA staff, 2009). Due to the inconsistency ofthis method, a conservative incorporation depth of0 (zero) inches is used.



## 26

### Appendix C: Example PRZMlEXAMS Output Files

27

### MS Com- Aerial Application
stored as MSCRnA.out Chemical: Pyraclostrobin PRZM environment: MScomSTD.txt modified Tueday, 29 May 2007 at 12:57:40 EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:08 Metfile: w03940.dvf modified Tueday, 26 August 2008 at 05: 14:14 Water segment concentrations (ppb)
Year Peak 96 hr 

### 21 Day 60 Day 90 Day Yearly 1961 0.461 0.3241 0.1295 0.094090.06759 0.02221 1962 0.275 0.1878 0.06892 0.02616 0.0232 0.01223 1963 0.5203 0.3618 0.1371 0.090890.06191 0.02173 1964 0.3804 0.2577 0.09207 0.05016 0.0468 0.02967 1965 0.5296 0.3708 0.1499 0.0635 0.044160.02088 1966 0.3456 0.2472 0.1096 0.05048 0.0465 0.02075 1967 0.6776 0.4654 0.1667 0.0856 0.0621 0.02262 1968 0.2787 0.1892 0.06563 0.028 0.0244 0.01377 1969 0.2741 0.2022 0.07112 0.03667 0.03606 0.01572 1970 0.3547 0.2458 0.08675 0.05765 0.04882 0.02336 1971 0.2758 0.1876 0.09351 0.05431 0.04567 0.0247 1972 0.2742 0.1867 0.072740.029120.023140.01543 1973 0.2753 0.1882 0.065860.03045 0.02427 0.01693 1974 0.5928 0.4104 0.1808 0.06842 0.05207 0.02722 1975 0.3598 0.2482 0.1504 0.09606 0.08287 0.03063 1976 0.4185 0.2864 0.1017 0.06588 0.04905 0.02125 1977 0.3003 0.2058 0.106 0.0608 0.046190.02318 1978 0.278 0.1879 0.06465 0.03577 0.0295 0.01792 1979 0.841 0.5793 0.2386 0.1529 0.1126 0.04939 1980 0.2758 0.1863 0.0639 0.0351 0.03287 0.01788 1981 0.6535 0.4504 0.1618 0.1082 0.07552 0.02489 1982 0.6518 0.4532 0.1782 0.1154 0.09221 0.03805 1983 0.2799 0.1927 0.071760.0374 0.03255 0.02444 1984 0.275 0.1856 0.064970.05151 0.047640.02241 1985 0.4033 0.2779 0.1023 0.05168 0.05134 0.02052 1986 0.3486 0.2543 0.1219 0.0682 0.05001 0.02127 1987 0.4838 0.3866 0.1573 0.08013 0.05876 0.02114 1988 0.274 0.1853 0.06377 0.03936 0.03375 0.01901 1989 0.7166 0.5061 0.2748 0.119 0.08328 0.02874 1990 0.2795 0.1888 0.06469 0.03362 0.02455 0.01787
Sorted results Prob. Peak 96 hr 

### 21 Day 60 Day 90 Day Yearly 0.032258064516129 0.841 0.5793 0.2748 0.1529 0.1126 0.04939 0.0645161290322581 0.7166 0.5061 0.2386 0.119 0.09221 0.03805 0.0967741935483871 0.6776 0.4654 0.1808 0.1154 0.08328 0.03063 0.129032258064516 0.6535 0.4532 0.1782 0.1082 0.08287 0.02967 0.161290322580645 0.6518 0.4504 0.1667 0.09606 0.07552 0.02874 0.193548387096774 0.5928 0.4104 0.1618 0.09409 0.06759 0.02722 0.225806451612903 0.5296 0.3866 0.1573 0.090890.0621 0.02489 0.258064516129032 0.5203 0.3708 0.1504 0.0856 0.06191 0.0247 0.290322580645161 0.4838 0.3618 0.1499 0.08013 0.05876 0.02444 0.32258064516129 0.461 0.3241 0.1371 0.06842 0.05207 0.02336 0.354838709677419 0.4185 0.2864 0.1295 0.0682 0.051340.02318



## 28

### 0.387096774193548 0.4033 0.2779 0.1219 0.06588 0.05001 0.02262 0.419354838709677 0.3804 0.2577 0.1096 0.0635 0.04905 0.02241 0.451612903225806 0.3598 0.2543 0.106 0.0608 0.04882 0.02221 0.483870967741936 0.3547 0.2482 0.1023 0.05765 0.04764 0.02173 0.516129032258065 0.3486 0.2472 0.1017 0.05431 0.0468 0.02127 0.548387096774194 0.3456 0.2458 0.09351 0.05168 0.0465 0.02125 0.580645161290323 0.3003 0.2058 0.092070.05151 0.046190.02114 0.612903225806452 0.2799 0.2022 0.08675 0.05048 0.04567 0.02088 0.645161290322581 0.2795 0.1927 0.072740.050160.044160.02075 0.67741935483871 0.2787 0.1892 0.07176 0.03936 0.03606 0.02052 0.709677419354839 0.278 0.1888 0.07112 0.0374 0.03375 0.01901 0.741935483870968 0.2758 0.1882 0.06892 0.03667 0.03287 0.01792 0.774193548387097 0.2758 0.1879 0.06586 0.03577 0.03255 0.01788 0.806451612903226 ·0.2753 0.1878 0.06563 0.0351 0.0295 0.01787 0.838709677419355 0.275 0.1876 0.064970.033620.02455 0.01693 0.870967741935484 0.275 0.1867 0.06469 0.03045 0.0244 0.01572 0.903225806451613 0.2742 0.1863 0.06465 0.02912 0.02427 0.01543 0.935483870967742 0.2741 0.1856 0.0639 0.028 0.0232 0.01377 0.967741935483871 0.274 0.1853 0.06377 0.02616 0.02314 0.01223
0.1 0.67519 0.46418 0.18054 0.11468 0.083239 0.030534 Average ofyearly averages: 0.0228603333333333 Inputs generatedby pe5.pl- Novemeber2006
Data used for this run: Output File: MSCRnA Metfile: w03940.dvf PRZM scenario: MScomSTD.txt EXAMS environment file:pond298.exv Chemical Name: Pyrac1ostrobin Description Variable Name Value Units Comments Molecular weightmwt 388 glmol Henry's Law Const. henry 4.13E-9 atm-m-S/mol Vapor Pressure vapr 1.95E-8 torr Solubility sol 2.41 mglL Kd Kd mglL Koc Koc 9304 mglL Photolysis half-life kdp .08 days Half-life Aerobic Aquatic Metabolism kbacw 496.28 days Halfife Anaerobic Aquatic Metabolism kbacs 6.6 days Halfife Aerobic Soil Metabolism asm 248.14 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 em Application Rate: TAPP 0.112 kg/ha Application Efficiency: APPEFFO.95 fraction Spray Drift DRFT 0.05 fraction ofapplication rate applied to pond Application Date Date 01-06 dd/mm or dd/mmm or dd-mm or dd-mmm Record 17: FILTRA IPSCNDI UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run 

### IR EPA Pond



## 29

### Flag forrunoffcalc. RUNOFF none none, monthly ortotal(average ofentire run) 

### MN Sugar Beet

- Seed Treatment
stored as MNSugST.out Chemical: Pyraclostrobin PRZM environment: MNsugarbeetSTD.txt modified Tueday, 29 May 2007 at 12:57:22 EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05: 14:08 Metfile: w14914.dvf modified Tueday, 26 August 2008 at 05:15:16 Water segment concentrations (ppb) Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.3301 0.2355 0.1059 0.044690.031640.009657 1962 0.5678 0.414 0.189 0.099540.070950.01872 1963 0.1647 0.1176 0.053590.0209 0.022560.006412 1964 0.4593 0.3258 0.1265 0.047940.03871 0.01076 1965 0.078590.05604 0.02454 0.01571 0.0127 0.005361 1966 0.08883 0.06301 0.03404 0.0179 0.01245 0.004593 1967 0.02581 0.018590.009525 0.007001 0.005946 1968 0.023040.016790.009207 0.007606 0.006769 1969 0.2358 0.166 0.091130.040830.03241 0.01008 1970 0.1799 0.1266 0.04907 0.02098 0.01781 0.008465 1971 0.2464 0.1749 0.073170.0313 0.021240.01137 1972 0.1781 0.1263 0.0506 0.020390.016170.005642 1973 0.1113 0.07925 0.03403 0.025040.018370.005924 1974 0.2671 0.1893 0.081930.053550.037160.01428 1975 0.3913 0.3172 0.1856 0.077440.053680.01461 1976 0.007729 0.005828 0.002817 0.002079 0.0004062 1977 0.635 0.4451 0.1706 0.0862 0.076 0.02276 1978 0.3642 0.2575 0.1002 0.043960.03803 0.01081 1979 0.1034 0.0731 0.029370.020970.014660.007322 1980 0.1589 0.1126 0.050670.031760.022570.00664 1981 0.4889 0.3506 0.1666 0.072 0.049840.01278 1982 0.2595 0.1847 0.078580.03677 0.02686 0.008263 1983 0.1102 0.077190.041630.02 0.015560.005328 1984 0.7212 0.5146 0.2206 0.08655 0.057990.01942 1985 0.2583 0.1863 0.1031 0.058420.04577 0.01233 1986 0.2267 0.16 0.06396 0.0322 0.02568 0.008676 1987 0.1574 0.112 0.061390.024420.021750.005959 1988 0.0733 0.055290.021780.0135 0.009827 0.003167 1989 0.1784 0.1361 0.061110.030170.021160.008086 1990 0.2641 0.1882 0.093680.047790.032760.009079 0.001623 0.00271

0.001489

Sorted results Prob. Peak 96 hr 

### 0.032258064516129 0.0645161290322581 0.0967741935483871 0.129032258064516 0.161290322580645 0.193548387096774 0.225806451612903 0.258064516129032 0.290322580645161 0.32258064516129 21 Day 0.7212 0.635 0.5678 0.4889 0.4593 0.3913 0.3642 0.3301 0.2671 0.2641 60 Day 0.5146 0.4451 0.414 0.3506 0.3258 0.3172 0.2575 0.2355 0.1893 0.1882 90 Day Yearly 0.2206 0.09954 0.076 0.02276 0.189 0.08655 0.07095 0.01942 0.1856 0.0862 0.057990.01872 0.1706 0.077440.05368 0.01461 0.1666 0.072 0.049840.01428 0.1265 0.05842 0.04577 0.01278 0.1059 0.053550.03871 0.01233 0.1031 0.047940.03803 O.oI137 0.1002 0.047790.037160.01081 0.09368 0.04469 0.03276 0.0 1076



### 30

### 0.354838709677419 0.2595 0.1863 0.09113 0.043960.03241 0.01008 0.387096774193548 0.2583 0.1847 0.08193 0.04083 0.031640.009657 0.419354838709677 0.2464 0.1749 0.07858 0.03677 0.02686 0.009079 0.451612903225806 0.2358 0.166 0.07317 0.0322 0.02568 0.008676 0.483870967741936 0.2267 0.16 0.06396 0.03176 0.02257 0.008465 0.516129032258065 0.1799 0.1361 0.06139 0.0313 0.02256 0.008263 0.548387096774194 0.1784 0.1266 0.06111 0.030170.021750.008086 0.580645161290323 0.1781 0.1263 0.053590.02504 0.02124 0.007322 0.612903225806452 0.1647 0.1176 0.050670.024420.02116 0,00664 0.645161290322581 0.1589 0.1126 0.0506 0.020980.018370.006412 0.67741935483871 0.1574 0.112 0.049070.020970.01781 0.005959 0.709677419354839 0.1113 0.07925 0.04163 0.0209 0.016170.005924 0.741935483870968 0.1102 0.077190.034040.020390.015560.005642 0.774193548387097 0.1034 0.0731 0.03403 0.02 0.01466 0.005361 0.806451612903226 0.088830.063010.029370.0179 0.0127 0.005328 0.838709677419355 0.078590.056040.024540.01571 0.01245 0.004593 0.870967741935484 0.0733 0.055290.021780.0135 0.009827 0.003167 0.903225806451613 0.02581 0.018590.009525 0.007606 0.006769 0.00271 0.935483870967742 0.023040.016790.009207 0.007001 0.005946 0.001623 0.967741935483871 0.007729 0.005828 0.002817 0.002079 0.001489 0.0004062 0.1 0.55991 0.407660.1841 0.085324 0.057559 0.018309 Average ofyearly averages: 0.00904110666666667 Inputs generated bype5.pl- Novemeber 2006 Data used for this run: Output File: MNSugST Metfile: w14914.dvf PRZM scenario: MNsugarbeetSTD.txt EXAMS environment file: pond298.exv Chemical Name: Pyraclostrobin Description Variable Name Value Units Comments Molecularweightmwt 388 g/mol Henry's Law Const. henry 4.13E-9 atm-m/S/rnol Vapor Pressure vapr 1.95E-8 torr Solubility sol 2.41 mg/L Kd Kd mg/L Koc Koc 9304 mg/L Photolysis half-life kdp .08 days Half-life Aerobic Aquatic Metabolism kbacw 496.28 days Halfife Anaerobic Aquatic Metabolism kbacs 6.6 days Halfife Aerobic Soil Metabolism asm 248.14 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 4 integer See PRZM manual Incorporation Depth: DEPI 0.635 em Application Rate: TAPP 0.118 kg/ha Application Efficiency: APPEFF 1.0 fraction Spray Drift DRFT 0.0 fraction of application rate applied to pond Application Date Date 01-05 dd/mm or dd/mmm or dd-rnm or dd-mmm Record 17: FILTRA IPSCND1 UPTKF Record 18: PLVKRT



### 31

### PLDKRT FEXTRC Flag for Index Res. Run Flag forrunoffcalc. 

### o IR EPA Pond RUNOFF none none, monthly or total(average ofentire run)



### 32

### Appendix D: Risk Quotient Calculations

33The Risk Quotient Method 

### The Risk Quotient method is the approach used by EFED in estimating the risk to aquatic and terrestrial species. This method involves integrating the results ofexposure and ecotoxicity data. For this method, risk quotients (RQs) are calculated by dividing the exposure estimates by the acute or chronic ecotoxicity values. RQ 

### = EXPOSURE/TOXICITY 

### RQs are then compared to OPP's levels ofconcem (LOCs). These LOCs are criteria used by OPP to indicate potential risk to non-target organisms and the need to consider regulatory action. EFED has defined LOCs for acute risk, potential restricted use classification and for endangered species. A description ofthe method and LOCs fornon-target organisms is presented in the following Table.



### 34Table D-I-Risk QuotientValues and Meanings RiskPresumptioos aDdLOCs RiskPresumption RQ 

### LOC
Birds' Acute Risk EECILC,o or LO,oIsqft or LO,oIday 0.5 Acute Restricted Use EECILC,o or LO,oIsqft or LO'o/day (or LO,o< 50 rng/kg) 0.2 Acute Endangered Species EECILC,o or LO,oIsClft or LO'o/day 0.1 Chronic Risk EEC/NOAEC 1 Wild Mammals' Acute Risk EECILC,o or LO,oIsqft or LO,oIday 0.5 Acute Restricted Use EECILC,o or LO,oIsClft or LO,oIday (or LO,o< 50 rug/kg) 0.2 Acute Endangered Species EECILC,o or LO,oIsQftor LO,oIday 0.1 Chronic Risk EEC/NOAEC I Aquatic Animals'
Acute Risk EECILC,o or EC,o 0.5
Acute Restricted Use EECILC,o or EC,o 0.1
Acute Endangered Species EECILC,o or EC,o 0.05 Chronic Risk EEC/NOAEC I
Terrestrial and Semi-Aquatic Plants
Acute Risk EEC/EC2, I
Acute Endangered Species EEC/ECo,or NOAEC I Aquatic Plants'
Acute Risk EEC/EC,o 1
Acute Endangered Species EEC/ECo, or NOAEC I

1 LO,oIsqft = (mg/sqft) I (LO,o

### *wt. ofanimal)

LO,oIday = (mg oftoxicant consumed/day) I (LO,o

### * wt. ofanimal) 2 EEC = (ppm or ppb) in wa



### 35Table D2- Coronet (seed treatment to Canola and Crambe 0.012 Ibs ai/acre) and aquatic risk quotients Aquatic Acute Chronic Peak 21-day 60-Day Acute Chronic LOC Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence Value (ug/L) (ug/L) (ug/l.) (ug/L) (1lg!L) Bluegill LC,o 96 N/A 0.38 0.11 0.06 0.03 N/A None Sunfish hr: I\.4 (LepomismacrochirusColdwater fish) Rainbow Trout LC,o 96 N/A 0.38 0.11 0.06 0.061 Yes Listed (Oncorhynchus hr: 6.2 Species OC mykiss-Warm- exceeded water fish) Fathead N/A NOAEC=4.14 0.38 0.11 0.06 0.01 None Minnow (Pimephales promelasi Rainbow Trout N/A NOAEC-2.35 0.38 0.11 0.06 0.03 None (Oncorhynchus mykiss) Sheepshead LC,o 96 hr NOAEC=10.8 0.38 0.11 0.06 <0.01 <0.01 None Minnow : 76.9 (CyprinodonvariegatesSalt-Water Fish) Daphnia EC,o 48 hr 0.38 0.11 0.06 0.024 NO magna : 15.7 a.i. (14.2-17.4)**~g/L** Daphnia NOAEC : 4 ug 0.38 0.11 0.06 0028 None magna a.i. /L
EC,o 96 hr 0.38 0.11 0.06 0.034 None Eastern Oyster : 12.5 (Crassostrea a.i.(9.2- virginica) 17.1) Mollusk Shell**~g/L** Deposition LC,o 96 hr 0.38 0.11 0.06 0.09 Yes Acute Mysid Shrimp : 4.16 Listed (Americamysis Species Use bahia) LOCs are exceeded Mysid Shrimp NOAEC=\.06! 0.38 0.11 0.06 0.06 None (Americamysis bahia)



### 36Table D 3- Coronet (seed treatment to Canola and Crambe O.0121bs at/acre) and aquatic plantriskquotients Aquatic Plants Toxicity Value (p.g/L) PeakEEC Value Risk QuotientValue Aquatic PlantLOC (J12!L) Exceedence? 0.38 <0.01(when using the None Freshwater Green Algae ECso96 hr: 152 (143- ECsoValue) and 0.03 (Pseudokirchneriella 162) ug a.i. /L (when using the subcapitata) (biomass) NOAEC value) NOAEC: 14.0 ug a.i./L (Biomass and Number of cells/ml) 0.38 <0.01 None Freshwater Blue-Green ECso 120 hr : > 1780 Algae ug a.i./L (Anabaenafios-aquae) (No. of cells/ml and growth rate) NOAEC : 1780 ug a.i./L (Growth rate and Number of cells/ml) 0.38 0.32 None Freshwater diatoms ECso 120 hr : 1.50 (Naviculapelliculosa) «1.18

- 3.83) ug a.i./L (No. ofcells/ml) NOAEC: 1.18 ug a.i./L (Number of cells/ml and Growth rate) 0.38 <0.01 (when using the None Marine diatoms ECso 120 hr: 64.7 (50 ECso value) and 0.038 (Skeletonema costatum)

- 84) ug a.i./L (No. of (when using the cells/ml) NOAEC value) NOAEC: 1O.01lg a.i./L (Number of cells/ml) Duckweed 

### sc., 14 days : 1720 0.38 <0.01 when using either None (Lemna gibba) ug a.i./L the ECsoor NOAEC (Plant biomass) Values NOAEC : 896 ug a.i./L (Number of normal fronds and plant biomass)



### 37Table D4-Aquatic Organism Risk Quotients of BAS 500 20 F (I application at O.llbs ai/acre applied to corn (field, pop, and seed production Aquatic Acute Chronic Peak 21-day 60-Day Acute Chronic LOC Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence Value (Ilg/L) (ug/L) (ug/L) (ug/L) (ug/L) Bluegill LC5096 N/A 0.68 0.18 0.11 0.06 N/A Acute Listed Sunfish hr: 11.4 Species (Lepomis LOC macrochirus- Exceeded Coldwater fish) Rainbow Trout LC,o 96 N/A 0.68 018 0.11 0.11 Yes Acute (Oncorhynchus hr: 6.2 Restricted mykiss-Warm- Use and water fish) Acute Listed Species OC exceeded Fathead N/A NOAEC-4.14 0.68 0.18 0.11 0.03 None Minnow (Pimepha/es promelasy Rainbow Trout N/A NOAEC-2.35 0.68 018 0.11 0.05 None (Oncorhynchus mykiss) Sheepshead LC,o 96 hr NOAEC=10.8 0.68 0.18 0.11 <0.01 0.01 None Minnow : 76.9 (CyprinodonvariegatesSalt-Water Fish) Daphnia EC,o 48 hr 0.68 0.18 0.11 0.043 None magna : 15.7 a.i. (14.2-17.4) Ilg/L Daphnia NOAEC: 4 ug 0.68 0.18 0.11 0.05 None magna a.i. /L
EC,o 96 hr 0.68 0.18 0.11 0.054 Acute Listed Eastern Oyster : 12.5 Species (Crassostrea a.i.(9.2- LOC virginica) 17.1) exceeded Mollusk Shell J.lg/L Deposition LC,o 96 hr 0.68 018 0.11 0.20 Acute Mysid Shrimp : 4.16 Restricted (Americamysis Use and bahia) Acute Listed Species LOCs are exceeded Mysid Shrimp NOAEC=1.06 1 0.68 0.18 0.11 0.20 None (Americamysis bahia)



### 38Table D5-Aquatic Plants Risk Quotients for BAS 500 20 F (I application at O.llbs ai/acre applied to corn (field, pop, and seed production) Aquatic Plants Toxicity Value (J1gIL) PeakEEC Value Risk QuotientValue Aquatic Plant LOC (112!L) Exceedence? 0.68 <0.01(when using the None Freshwater Green Algae EC,o 96 hr : 152 (143- EC,o Value) and 0.05 (Pseudokirchneriella 162) ug a.i. /L (when using the subcapitata) (biomass) NOAEC value) NOAEC: 14.0 ug a.i.lL (Biomass and Number ofcells/ml) 0.68 <0.01 None Freshwater Blue-Green EC,o 120 hr: > 1780 Algae ug a.i./L (Anabaenajlos-aquae) (No. of cells/ml and growth rate) NOAEC : 1780 ug a.i./L (Growth rate and Numberof cells/ml) 0.68 0.5 (when using the None Freshwater diatoms EC,o 120 hr : 1.50 EC,o Value) and 0.60 (Naviculapelliculosa) «1.18 - 3.83) ug a.i./L (when using the (No. of cells/ml) NOAEC value) NOAEC: 1.18 ug a.i.lL (Number ofcells/ml and Growth rate) 0.68 0.01 (when using the None Marine diatoms EC,o 120hr: 64.7 (50 EC,o value) and 0.068 (Skeletonema costatum) - 84) ug a.i./L (No. of (when using the cells/ml) NOAEC value) NOAEC: 10.01lg a.i.lL (Number of cells/ml) Duckweed EC,o 14 days : 1720 0.68 <0.01 when using either None (Lemna gibba) ug a.i./L the EC,o or NOAEC (Plant biomass) Values NOAEC : 896 ug a.i./L (Number of normal fronds and plant biomass)



### 39Table D6-Aquatic Orzamsm Risk Quotients of STAMINA to sweet corn seeds (O.003Ibs. a.i.lacre) Aquatic Acute Chronic Peak 2I-day 60-Day Acute Chronic LOC Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence Value**(~g/L)** (ug/l.) (ug/L) (ug/L) [ug/L) Bluegill LC,o 96 N/A 0.02 0.007 0.003 <0.01 N/A None Sunfish hr: 11.4 (LepomismacrochirusColdwater fish) Rainbow Trout LCso96 N/A 0.02 0.007 0.003 <0.01 None (Oncorhynchus hr: 6.2mykiss-Warrnwater fish) Fathead N/A NOAEC=4.14 0.02 0.007 0.003 <0.01 None Minnow (Pimephales promelasi Rainbow Trout N/A NOAEC-2.35 0.02 0.007 0.003 <0.01 None (Oncorhynchus mykiss) Sheepshead LC,o 96 hr NOAEC=10.8 0.02 0.007 0.003 <0.01 <0.01 None Minnow : 76.9 (CyprinodonvariegatesSalt-Water Fish) Daphnia ECso48 hr 0.02 0.007 0.003 <0.01 None magna : 15.7 a.i. (14.2-17.4)**~g/L** Daphnia NOAEC : 4 ug 0.02 0.007 0.003 <0.01 None magna a.i. /L
ECso96 hr 0.02 0.007 0.003 <0.01 Eastern Oyster : 12.5 (Crassostrea a.i.(9.2- virginica) 17.1) Mollusk Shell**~g/L** Deposition LC,o 96 hr 0.02 0.007 0.003 <0.01 Mysid Shrimp : 4.16 (Americamysis bahia) Mysid Shrimp NOAEC=106 1 0.02 0.007 0.003 <0.01 None (Americamysis bahia)



### 40Table D7-Aquatic Plants Risk Quotients ofSTAMINA to sweetcorn seeds (O.003Ibs. a.iJacre) usingPeakEEC values Aquatic Plants Toxicity Value (1tWL) PeakEEC Value Risk QuotientValue Aquatic Plant LOC (I1lUL) Exceedence? 0.02 <O.OI(when using the None Freshwater Green Algae EC,o 96 hr: 152 (143- ECsoor NOAEC (Pseudokirchneriella 162) ug a.i. /L values) subcapitatai (biomass) NOAEC: 14.0 ug a.i./L (Biomass and Number of cells/ml) 0.02 <0.01 None Freshwater Blue-Green ECso 120hr: > 1780 Algae ug a.i./L (Anabaenaflos-aquaei (No. ofcells/ml and growth rate) NOAEC : 1780 ug a.i./L (Growth rate and Number of cells/ml) 0.02 0.013 (when using the None Freshwater diatoms ECso 120 hr : 1.50 ECsoValue) and 0.017 (Naviculapelliculosa) «1.18 - 3.83) ug a.i./L (when using the (No. of cells/ml) NOAEC value) NOAEC: 1.18 ug a.i./L (Number of cells/ml and Growth rate) 0.02 <0.01 (when using the None Marine diatoms ECso 120hr: 64.7 (50 ECso value) and 0.002 (Skeletonema costatumi - 84) ug a.i./L (No. of (when using the cells/ml) NOAEC value) NOAEC : 10.0 ug a.i./L (Number ofcells/ml) Duckweed ECso 14days: 1720 0.02 <0.01 when using either None (Lemna gibba) ug a.i./L the ECsoor NOAEC (Plant biomass) Values NOAEC : 896 ug a.i.lL (Number ofnormal fronds and plant biomass)



### 41Table D8-AQuatic Organism Risk Quotients of STAMINA to sugar beets seeds (0.104Ibs. a.i.zacre) Aquatic Acute Chronic Peak 21-day 60-Day Acute Chronic LOC Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence Value (flglL) (ug/L) (ug/L) (flg/L) (ug/l.) Bluegill LCso96 N/A 0.56 018 0.06 0.05 N/A Acute Listed Sunfish hr: 11.4 Species (Lepomis LOC is macrochirus- triggered Coldwater fish) Rainbow Trout LCso96 N/A 0.56 0.18 0.06 0.10 Acute (Oncorhynchus hr: 6.2 Restricted mykiss-Warm- Use LOC is water fish) triggered and Acute Listed Species Use is exceeded Fathead N/A NOAEC-4.14 0.56 0.18 0.06 0.01 None Minnow (Pimephales promelasi Rainbow Trout N/A NOAEC=2.35 0.56 0.18 0.06 0.03 None (Oncorhynchus mykiss) Sheepshead LCso96 hr NOAEC-IO.8 0.56 0.18 0.06 <0.01 <0.01 None Minnow : 76.9 (CyprinodonvariegatesSalt-Water Fish) Daphnia ECso48 hr 0.56 018 0.06 0.04 None magna : 15.7 a.i. (14.2-174) J.lg/L Daphnia NOAEC : 4 ug 0.56 0.18 0.06 0.05 None magna a.i. /L

ECso96 hr 0.56 0.18 0.06 0.04 None Eastern Oyster : 12.5 (Crassostrea a.i.(9.2- virginicai 17.1) Mollusk Shell flglL Deposition LCso96 hr 0.56 0.18 0.06 0.13 Acute Mysid Shrimp : 4.16 Restricted iAmericamysis Use and bahia) Acute Listed Species LOCs are exceeded Mysid Shrimp NOAEC=1.06 1 0.56 0.18 0.06 017 None (Americamysis bahia)



## 42Table D9-AQuatic Plants Risk Quotients of STAMINA to suaar beet seeds 0.104 lbs, a.i.lacre) uslna Peak EEC values Aquatic Plants Toxicity Value (!Jg/L) Peak EEC Value Risk Quotient Value Aquatic Plant LOC (JlI!:/L) Exceedence? 0.56 <0.01(when using the None FreshwaterGreen Algae ECso96 hr: 152 (143- ECso ) and0.04 (whe (Pseudokirchneriella 162) ug a.i. /L using the NOAEC subcapitata) (biomass) values) NOAEC: 14.0 ug a.i./L (Biomass andNumber ofcells/ml) 0.56 <0.01 None Freshwater Blue-Green ECso 120 hr : > 1780 Algae ug a.i./L (Anabaenaflos-aquaej (No. of cells/ml and growth rate) NOAEC : 1780 ug a.i./L (Growth rate and Number ofcells/ml) 0.56 0.40 (when using the None Freshwaterdiatoms ECso 120 hr : 1.50 ECsoValue) and 0.50 (Naviculapelliculosa) «1.18

- 3.83) ug a.i./L (when using the (No. ofcells/ml) NOAEC value) NOAEC: 1.18 ug a.i./L (Number ofcells/ml and Growth rate) 0.56 <0.01 (when using the None Marine diatoms ECso 120 hr : 64.7 (50 ECso value) and 0.056 (Skeletonema costatum)

- 84) Ilg a.i./L (No. of (when using the cells/ml) NOAEC value) NOAEC: 10.0 ug a.i./L (Number ofcells/ml) Duckweed ECso 14days: 1720 0.56 <0.01 when using either None (Lemna gibba) ug a.i.zl, the ECsoor NOAEC (Plant biomass) Values NOAEC : 896 ug a.i./L (Number of normal fronds and plant biomass)



### 43

### Appendix E: T-REX Modeling



## 44Acute 0.00 0.00 0.00 0.00 Note: To provide risk management with the maximum possible information, it is recommended that both the dose-based and concentration-based RQs be calculated when data are available


### 45Pyraclostrobin Mammalian Results Corn

Acute #DIVIOI #DIVIOI #DIV/OI #DIV/OI Chronic 0.04 0.02 0.02 0.00



### 46 Upper bound Kenasa Residues**~"~-'----'-----**

## 47 DoItIIlnputs
.... in blua

.0200**OOסס0. OOסס0.** **OOסס0. OOסס0.**  0.1000 0.0000 0.0100**OOסס0.** 0.0000 0.0000**OOסס0. OOסס0.**  0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 1.3300 0.0000 0.00000.00 200.00 50.82 42.37 0.03 

# -- 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 1000.00 253.09 211.87 0.08 0.00 0.00 0.00 0.00 0.00 0.00 100.00 25.31 21.19 0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.11 13300.00 3366.08 2817.90 1.11 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

Acutll .1 Acutll • 2 Chronic Acutll,l Acutll Chronic

0.05 0.00 0.19 0.00 0.00 0.34 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.24 0.00 0.94 0.02 0.00 1.72 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.00 0.09 0.00 0.00 0.17 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3.24 0.05 12.52 0.26 0.01 22.93 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Acuttl RQ'" '" (mg allkg.bw/day) , LD50 Acute RQ • mg ai n·2 I(LD50*bw) Chronic RQ '" mg kg·1 saed I NOEC



### 48

### Appendix F: Terr-PlantModeling for Pyraclostrobinused as an encapsulation and as a seed treatment:

49Table 1. ChemiealldentitY. Chemical Name 

### IPyraclostrobin PC code 99100

Use Corn Application Method Encapsulation

Application Form Spray :SOlUbility In water (ppm) 2.41
ITable 2. Input parameters used to derive EECs. 

## I Input Parameter Symbol Value Units Application Rate A 0.1 Ibs ailA

Incorporation I 1 none Runoff Fraction R 0.02 I none

Drift Fraction D 0.05 none
Table 3. EECsfor Pvraclostrobin. Units in Ibs ailA. Description Equation EEC Runoff to dry areas (A1I)*R 0.002 Runoff to semi-aquatic areas (A1I)*R*10 0.02 Spray drift A*D 0.005 Total for dry areas «A1I)*R)+(A*D) 0.007 Total for semi-aquatic areas ((A1I)*R*101+(A*DI 0.025
Table 4. Plant .urvival and growth data used for RQ derivation. Units are in Ibs ailA. Seedling Emergence Vegetative Vigor Plant type EC25 NOAEC EC25 NOAEC Monocot 0.78 0.3 0.6 0.6

Dicot 0.15 0.075 0.44 0.038
Table 5. RQ values for plants in dry and semi-aquatic areas exposed 

### to Pyraelostrobin through runoff andlorspray drift.* Plant Type Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1 Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 0.17 <0.1 Dicot listed <0.1 0.33 <0.1 1*lfRQ > 1.0, the LOC IS exceeded, resulting In potential for risk to that plant group.



### 50T.ble 1. ChemIcaIlcIentitY. Chemical Name pyraclostrobin PC code 99100

Use Sweet Corn Application Method ground incorporation

Application Form liquid SOlubility In Water (ppm) 2.41
T.ble2.ln '

# ..... !:_. Input Parameter Symbol Value Units Application Rate A 0.0034 Ibs ai/A

Incorporation I 3 none Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none
nble 3. ESC_fer

### lWfIotoetro...... Unitt In

.... •UA. Description Eauation EEC Runoff to dry areas (AlI)*R 1.13333E-05 Runoff to semi-aquatic areas (AlI)*R*10 0.000113333 Spray drift A*D 0.000034 Total for dry areas «AlI)*R)+{A*D) 4.53333E-05 Total for semi-aauatic areas ((AlI)*R*10)+(A*D) 0.000147333 

## rr
..... 4. Plant"'l'YiWll 

### ana ","for ,VatII 'N'" .- ..IIA Seedling Emergence Vegetative Vigor Planttype EC25 NOAEC EC25 NOAEC Monocot 0.118 0.118 0.118 0.118

Dicot 0.118 0.118 0.118 0.118
T.b

.... 

### RaV

...... ,.,••

### indry

### and semi-aqu.tIc

.......

### Qosed

### to pyrac!oetrobln through 

### "lnoff land/or

..naYddft.· PlantType Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1 Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1 Dicot listed <0.1 <0.1 <0.1

* 

### I If RQ > 1.0, the LOC IS exceeded, resulting In potentialfor risk to that plant group.



### 51lTable 1. Chemical IdentitY. Chemical Name pyraclostrobin PC code 99100

Use Sugar beet seeds Application Method 

### Iground incorporation

Application Form liquid SOlubility In water (ppm) 2.41
Table 2. InDutDara

..... 

### used todetiveEEGe. Input Parameter Svmbol Value Units Application Rate A 0.1056 Ibs ailA

Incorporation I 1 none Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none Table 3. EECs for IM1lclosU'O,"n. Un" in Ibs aiiA. Description Equation EEC Runoff to dry areas (AlI)*R 0.001056 Runoff to semi-aquatic areas (AlI)*R*10 0.01056 Spray drift A*D 0.001056 Total for dry areas «A1I)*R)+(A*D) 0.002112 Total forsemi-aquatic areas llAlI)*R*10)+(A*D) 0.011616
Table 

### 4. P

..nt

....

### Afiv.land lIJ'OWth

### .ata

..... fOrRG dertvaaon.

# u..i

.... in

....

### li#.A. Seedling Emergence Vegetative Vigor Planttype EC25 NOAEC EC25 NOAEC Monocot 0.118 0.118 0.118 0.118

Dicot 0.118 0.118 0.118 0.118
Table 5. 

### RClVJIUM for

### plan. indryand

..mf-aquatlC

.... 

### exposectto pyta8

...... .............. anctlouprav drift.* PlantType Listed Status Dry Semi·AQuatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1 Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1 Dicot listed <0.1 <0.1 <0.1

* 

### I If RQ > 1.0, the LOC IS exceeded, resulting In potential for riskto that plant group.



### 52Tlble 1. ChemlCllldentltv. Chemical Name pyraclostrobin PC code 99100

Use Rapeseeds Application Method 

### Iground incorporation

Application Form liquid SOlubility In Water (ppm) 2.41
lTabieI. InDut

............ ueecI 

### to eMrlVeEEC•• Input Parameter Svmbol Value Units Application Rate A 0.0096 Ibs ailA

Incorporation I 1 none Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none IT.b

.. 

### 3.

.pyr.cIq$trobln. 

## un'" In 

### I• •UA. Description Equation EEC Runoff to dry areas (AlI)*R 0.000096 Runoff to semi-aquatlc areas (AlI)*R*10 0.00096 Spray drift A*D 0.000096 Total for dry areas «AlI)*R)+(A*D) 0.000192 Total for semi-aQuatic areas ((AlI)*R*10)+(A*D) 0.001056
TID"

### 4. Pllnt lurvlvalanet llrowth dl. 

### ueecI for

### RCfderiVation. Un'" are In lbe aUA.
Planttype Seedling Emergence Vegetative Vigor EC25 NOAEC EC25 NOAEC Monocot 0.118 0.118 0.118 0.118

Dicot 0.118 0.118 0.118 0.118
Tlble S.RQ Yalu

.. for pllnts in dtyand aeml-aquatic are
.. exposed to pyraciOllrobin through runoff and/or IDI'8Ydrift.* PlantTVDe Listed Status Drv Semi·Aauatic SDrav Drift

Monocot non-listed <0.1 <0.1 <0.1 Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1 Dicot listed <0.1 <0.1 <0.1

* 

### I If RQ > 1.0, the LOC IS exceeded, resulting In potential for risk to that plant group.



### 53

### Appendix G: Ecotoxicological and Toxicological Summary

54

### Table G-l. Acute Toxic Effects ofPyraclostrobin on Freshwaterand Estuarine Fish. Species Study 

### % ToxicityEndpoint Toxicity Study MRID# Guideline A.I. (Confidence Classification Classificati Interval) on Freshwater Fish Bluegill Sunfish 72-la 97 LC,o 96 hr : 11.4 (9.52- Very highly toxic Supplemental 45118414 (Lepomis 15.9) ug a.i./L macrochirus) NOAEC : 6.08 ug a.i. /L (mortality and symptoms)
Rainbow Trout 72-lc 97 LC,o 96 hr : 6.2 (5.25- Very highly toxic Acceptable 45118413 (Oncorhynchus 8.97) ug a.i. /L mykiss) NOAEC : 3.60 ug a.i. /L (mortality and discoloration)
EstuarinelMarine Fish Sheepshead Minnow 72-3a 97 LC,o 96 hr : 76.9 (53.5- Very highly toxic Supplemental 45118415 iCyprinodon 87.9) ug a.i. /L variegatus) NOAEC : 53.5 /lg a.i./L (survival)

Table G-2 Acute Toxic Effects ofPyraclostrobin degradation products on rainbow trout (Oncorhynchus mykiss). Nameof Study %A.I. Toxicity Endpoint Toxicity Study MRID# transformation Product Guideline (Confidence Interval) Classification Classification
BF 500-11 72-lc 99 LC,o 96 hr :>99.18 mg a.i. Practically non- Acceptable 45118416 (Reg. No. 411847) /L toxic NOAEC : 9.98 mg a.i. /L (apathy etc.)
BF 500-13 72-lc 100 LC,o 96 hr :75 mg a.i./L Slightly toxic Acceptable 45118417 (Reg. No. 412785) NOAEC : 9.3 mg a.i. /L (convulsions etc.)
BF 500-14 72-lc 96 LC,o 96 hr :57 mg a.i./L Slightly toxic Acceptable 45118418 (Reg. No. 413038) NOAEC : 39.4 mg a.i. /L (mortality)



### 55Table G-3 Chronic Toxicity Effects ofPyraclostrobin in Fish. Species Study % A.I. Toxicity Endpoint Study MRID# Guideline (Confidence Interval) Classification
Freshwater Fish
Fathead Minnow 72-4a 97.1 NOAEC: 4.14 ug a.i. /L Acceptable 45118420 (Pimephalespromelas) LOAEC: 8.37 ug a.i. /L (hatch/post hatch survival, bodyweight and length and sublethal symptoms)
Rainbow Trout 72-4a 99 NOAEC: 2.35 ug a.i. /L Supplemental 45118421 (Oncorhynchus mykiss) LOAEC: 6.42 ug a.i. /L (hatch/post hatch survival)

Estuarine/Marine Fish Sheepshead Minnow 72-4a 93.5 NOAEC: 10.8**~g** a.i. /L Acceptable 45420101 (Cyprinodon variegatus) LOAEC: 24.0 ug a.i. /L (hatch/post hatch survival)

Table G-4. Acute and Chronic Toxic Effects ofPyraclostrobin in Aquatic Invertebrates. 

### Species Study % A.I. Toxicity Endpoint Toxicity Study MRID# Guideline (Confidence Interval Classification' Classification
Freshwater Invertebrates Daphnia magna 72-2 97.1 EC50 48 hr: 15.7 a.i. (14.2- Very highly toxic Supplemental 45118407 17.4) ug/L NOAEC: 11.5 ug a.i. /L (immobility) Daphnia magna 72-4b 97.1 NOAEC : 4 ug a.i. /L


## - Supplemental 45118419 LOAEC: 8 ug a.i. /L (Number ofoffsprings produced, length and survival of daphnids)

EstuarinelMarine Invertebrates Eastern Oyster 72-3b 97.1 EC50 96 hr : 12.5 a.i.(9.2- Very highly toxic Acceptable 45118411 (Crassostrea 17.1) virginica)**~g/L** Mollusk Shell NOAEC : 4.06 ug a.i./L Deposition (shell deposition) Mysid Shrimp 72-3c 97.1 LC50 96 hr : 4.16 (3.64-5.97) Very highly toxic Acceptable 45118412 (Americamysis ug a.i./L bahia) NOAEC : 2.12 ug a.i./L (lethargy and mortality)

Table G-5. Acute Toxicity Studies of the transformation ProductofPyraclostrobin in Daphnia magna.



### 56Name of Study %A.I. Toxicity Endpoint Toxicity Study MRID# transfonnation Guideline (Confidence Interval) Classification Classification Product
BF 500-11 72-2 98.9 ECso48 hr : >100 Practically non- Supplemental 45118408 mg/L toxic NOEC: 100 mg/L (immobility)

BF 500-13 72-2 99.6 ECso48 hr : >I00 Practically non- Supplemental 45118409 mg/L toxic NOEC : 50 mg/L (immobility)

BF 500-14 72-2 96.1 ECso48 hr: >61.8 Slightly non-toxic Supplemental 45118410 mg/L NOEC: 2':61.8 mg/L (immobility)



### 57Table G-6. Acute Toxic Effects ofPyraclostrobin on Aquatic Plants. 

### Species Study 0/0 Toxicity Endpoint Study MRID# 

### Guideline 

### A.l. (Confidence Interval Classification

Nonvascular Plants
Freshwater Green Algae 123-2 97.1 ECso96 hr: 152 (143-162) ug a.i.1L Acceptable 45118722 (Pseudokirehnerie//a (biomass) subeapitata) NOAEC: 14.0 ug a.iJL (Biomass and Number of cells/ml)
Freshwater Blue-Green 123-2 97.1 ECso 120 hr : > 1780 ug a.i.IL Acceptable 45118716 Algae (No. of celIs/ml and growth rate) (Anabaenafios-aquae) NOAEC : 1780 ug a.i.1L (Growth rate and Number of celIs/m1)
Freshwater diatoms 123-2 97.1 ECso 120hr: 1.50 «118 - 3.83) ug a.i.IL Acceptable 45118721 (Navieu/apel/ieu/osa) (No. of celIs/ml) NOAEC: 1.18 J.lg a.i./L (Number ofcells/ml and Growth rate)
Marine diatoms 123-2 97.1 ECso 120 hr : 64.7 (50 - 84) ug a.i.1L (No. Acceptable 45118717 (Ske/etonema eostatum) of cells/ml)
NOAEC: 10.0 ug a.i.1L (Number ofcells/ml)
VascularPlant
Duckweed 123-2 97.1 ECso 14days: 1720 ug a.ilL Acceptable 45118715 Lemna gibba (Plant biomass)
NOAEC : 896 J.lg a.i./L (Number of normal fronds and plant biomass)



### 58Table G-7. The Toxicity of the Transformation Products ofPyraclostrobin in FreshwaterAlgae (Scenedesmus subspicatus).
Name of Study %A.I. Toxicity Endpoint Study MRID# transformation Guideline (Confidence Interval) Classification Product
BF 500-11 123-2 98.9 ECso72 hr: >1000,000 ug.a.i, /L Supplemental 45118718 (biomass; growth rate) NOAEC : 50,000 ug a.i.lL (Number of cells/ml)
BF 500-13 123-2 99.6 ECso72 hr : 66,000 ug.a.i, /L Supplemental 45118719 (biomass) NOAEC : 12,500 ug a.i./L (Number ofcells/ml)
BF 500-14 123-2 96.1 ECso72 hr : 46,600 ug.a.i, /L Supplemental 45118720 (biomass) NOAEC: 12,500 ug a.i./L (Number ofcells/ml)

Table G-8. Avian Toxicity Studies ofPyrac1ostrobin. 

### Species Study %A.I. ToxicityEndpoint Toxicity Study MRID# 

### Guideline (Confidence Interval) Classification Classification Bobwhite quail 71-la 97.1 LDso 14days: > 2000 Practically Acceptable 45118423 (Colinus mg/KgBW non-toxic virginianusy NOAEC: 500 mg/kg BW (body weight gain) Bobwhite quail 71-2a 98.7 LCso8 days: > 5000 mg/Kg Practically Acceptable 45118424 (Colinus diet non-toxic virginianusi NOAEC: 2500 mg/kg diet (body weight gain)
Mallard duck 71-2b 97.1 LCso8 days : > 5000 mg/Kg Practically Acceptable 45118425 (Anas diet non-toxic platyrhynchos) NOAEC: 625 mg/kg diet (body weight gain) Bobwhite quail 71-4a 98.7 NOAEC: 1061.5 mg/kg diet N/A Acceptable 45118426 (Colinus virginianus) Mallard duck 71-4b 98.7 NOAEC: 1062 mg a.i./kg N/A Acceptable 45118427 (Anas diet platyrhynchos)



### 59

### Table G-9 Mammalian Toxicity Studies ofPyraclostrobin. Species Study 0/0 

### Toxicity Endpoint Toxicity Study MRID# Guidelin A.I. (Confidence Interval) Classificati Classificati e on on Rats 81-1 98.5 LDso: 25000 mg/kg-bw (males and Category IV Acceptable 45118302 females) (Low toxicity)
Rats 81-1 23.4 LDso: 500-2000 mg/kg-bw (males) Category 11 Acceptable 45118303 (Headline LDso: 260 mg/kg-bw (females) (High EC) LDso: 500 mg/kg-bw (males and toxicity) females)

Rats 81-1 21 LDso: >2000 mg/kg-bw (males and Category 111 Acceptable 45118304 (Cabrio females) (Low EG) toxicity)



### Table G-IO. Chronic Mammalian Toxicity Studies ofPyraclostrobin. Species Study 0/0 

### Toxicity Endpoint Study Classification MRID# Guideline A.I. (Confidence Interval)
Rats 83-3a 98.9 Maternal: Acceptable-Guideline 45118325 INOAEL: 10 mg/kg/day lLOAEL 25 mg/kg/day Developmental 2NOAEL: 25 mg/kg/day 2LOAEL: 50 mglkg/day Rabbits 83-3b 98.9 Maternal: Acceptable-Guideline 45118326
1 NOAEL:5 mg/kg/day I LOAEL 10mglkg/day Developmental 3NOAEL: 5 mg/kg/day
3 LOAEL: 10 mglkg/day
Rats 83-4 98.7 NOAEL 2300 ppm Acceptable-Guideline 45118327 Two generation reproduction study (29-35 mglkgIBW) decreased body weight gam and reduced food consumption, reduced food efficiency. 2Increase in fetal and litter incidence ofdilated renal pelvis; increased incidence of cervical ribs with cartilage not present. 3 Increased resorptions per litter, increased post implantation loss and dams with total resorptions.



### 60

### Table G-ll. Non-target Insect Toxicity Studies ofPyraclostrobin. Species Study 0/0 

### Toxicity Endpoint Toxicity Study MRID# Guideline A.I. (Confidence Classification Classification Interval) Honey 141-1 97.09 LDso

### 48 

### hr: > 

### 100 ug Practically Acceptable 45118711 bees a.i./bee nontoxic (Apis 

### NOEC : :::-: 100 I!g mellifera) 

### a.i./bee (survival)

Table G-12. Terrestrial Plant Toxicity Studies ofPyraclostrobin. Species Study 0/0 

### Toxicity Endpoint Study MRID# Guideline A.I. (Confidence Interval) Classification Seedling Emergence, 122-1a 23.6 EC25

### 21 days Supplemental 45118713 Tier 1 (Monocots and Dicots): (Monocots and Dicots) 

### > 0.118 lb a.i. /acre (percent emergence, percent survival, phytotoxicity rating, plant height and dry weight)
NOEC : :::-: 0.118 lb a.i. /acre (Same parameters as above) Vegetative Vigor 122-1b 23.6 EC2s 

### 21 days Supplemental 45118714 Tier I (Monocots and Dicots): (Monocots and Dicots) 

### > 0.1181b a.i. /acre (Same parameters as in Emergence)
NOEC::::-: 0.1181b a.i. /acre (Same parameters as in Emergence)



### 61

### Appendix H: LOCATES Runs



## 62

# Species Counts by StateforIndicatedCrops

No species were excluded. Minimum of 1 Acre. All Medium Types Reported 

## corn (PR), cornforgrain, cornforgrain (irrigated), cornforsilage or greenchop, cornfor silage orgreenchop (irrigated), Sugar beet seeds, Sweet Corn seeds, andRapeseeds AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY



### Alabama The taxa Amphibian has 2 species co-occurring with indicated crops. The taxa Bird has 3 species co-occurring with indicated crops. The taxa Bivalve has 30 species co-occurring with indicated crops. The taxa Crustacean has 1 species co-occurring with indicated crops. The taxa Dicot has 10 species co-occurring with indicated crops. The taxa Ferns has 3 species co-occurring with indicated crops. The taxa Fish has 15 species co-occurring with indicated crops. The taxa Gastropod has 10 species co-occurring with indicated crops. The taxa Mammal has 4 species co-occurring with indicated crops. The taxa Monocot has 3 species co-occurring with indicated crops. The taxa Reptile has5 species co-occurring with indicated crops. 

### Arizona The taxa Amphibian has 2 species co-occurring with indicated crops. The taxa Bird has 8 species co-occurring with indicated crops. The taxa Dicot has 14 species co-occurring with indicated crops. The taxa Fish has 15 species co-occurring with indicated crops. The taxa Gastropod has 1 species co-occurring with indicated crops. The taxa Mammal has 8 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 2 species co-occurring with indicated crops.



### 63

### Arkansas The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 6 species co-occurring with indicated crops. The taxa Crustacean has 1 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Fish has 3 species co-occurring with indicated crops. The taxa Gastropod has 1 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. 

### California

The taxa Amphibian has 6 species co-occurring with indicated crops. The taxa Bird has 13 species co-occurring with indicated crops. The taxa Conf/cycds has 1 species co-occurring with indicated crops. The taxa Crustacean has 8 species co-occurring with indicated crops. The taxa Dicot has 136 species co-occurring with indicated crops. The taxa Fish has 25 species co-occurring with indicated crops. The taxa Gastropod has 2 species co-occurring with indicated crops. The taxa Insect has 15 species co-occurring with indicated crops. The taxa Mammal has 20 species co-occurring with indicated crops. The taxa Marine mml has 2 species co-occurring with indicated crops. The taxa Monocot has 15 species co-occurring with indicated crops. The taxa Reptile has 7 species co-occurring with indicated crops. 

### Colorado

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Dicot has 8 species co-occurring with indicated crops. The taxa Fish has 5 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. 

### Connecticut

The taxa Bird has 2 species co-occurring with indicated crops.

AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 

### 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,



## 64VA, WA, WV, WI, WY The taxa Bivalve has 1 species co-occurring with indicated crops. The taxa Dicot has 1 species co-occurring with indicated crops. The taxa Fish has 1 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. Delaware

The taxa Bird has 1 species co-occurring with indicated crops. The taxa Fish has 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. Florida

The taxa Amphibian has 2 species co-occurring with indicated crops. The taxa Bird has 8 species co-occurring with indicated crops. The taxa Bivalve has 7 species co-occurring with indicated crops. The taxa Conf/cycds has 1 species co-occurring with indicated crops. The taxa Crustacean has 1 species co-occurring with indicated crops. The taxa Dicot has 43 species co-occurring with indicated crops. The taxa Fish has 4 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Lichen has 1 species co-occurring with indicated crops. The taxa Mammal has 8 species co-occurring with indicated crops. The taxa Marine mml has 1 species co-occurring with indicated crops. The taxa Monocot has 3 species co-occurring with indicated crops. The taxa Reptile has 10 species co-occurring with indicated crops. Georgia

The taxa Amphibian has 2 species co-occurring with indicated crops.

AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 

### 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,



### 65VA, WA, WV, WI, WY The taxa Bird has 4 species co-occurring with indicated crops. The taxa Bivalve has 16 species co-occurring with indicated crops. The taxa Conf/cycds has 1 species co-occurring with indicated crops. The taxa Dicot has 11 species co-occurring with indicated crops. The taxa Ferns has 2 species co-occurring with indicated crops. The taxa Fish has 11 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops. The taxa Marine mml has 1 species co-occurring with indicated crops. The taxa Monocot has 6 species co-occurring with indicated crops. The taxa Reptile has 2 species co-occurring with indicated crops. Hawaii

The taxa Arachnid has 1 species co-occurring with indicated crops. The taxa Bird has 28 species co-occurring with indicated crops. The taxa Crustacean has 1 species co-occurring with indicated crops. The taxa Dicot has 216 species co-occurring with indicated crops. The taxa Ferns has 11 species co-occurring with indicated crops. The taxa Gastropod has 39 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Marine mml has 1 species co-occurring with indicated crops. The taxa Monocot has 21 species co-occurring with indicated crops. The taxa Reptile has 2 species co-occurring with indicated crops. Idaho

The taxa Bird has 1 species co-occurring with indicated crops. The taxa Dicot has 2 species co-occurring with indicated crops. The taxa Fish has 8 species co-occurring with indicated crops. The taxa Gastropod has 4 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops.



## 66AL, AK, 

### AZ.., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN,MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY

Illinois

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 7 species co-occurring with indicated crops. The taxa Crustacean has 

### 1 species co-occurring with indicated crops. The taxa Dicot has 7 species co-occurring with indicated crops. The taxa Fish has 

### 1 species co-occurring with indicated crops. The taxa Gastropod has 

### 1 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. Indiana

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 

### 11 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 

### 1 species co-occurring with indicated crops. The taxa Reptile has 

### 1 species co-occurring with indicated crops. Iowa

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 2 species co-occurring with indicated crops. The taxa Dicot has 3 species co-occurring with indicated crops. The taxa Ferns has 

### 1 species co-occurring with indicated crops. The taxa Fish has 2 species co-occurring with indicated crops. The taxa Gastropod has 

### 1 species co-occurring with indicated crops. The taxa Mammal has 

### 1 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. Kansas

The taxa Bird has 3 species co-occurring with indicated crops.



## 67AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY The taxa Dicot has 

### 1 species co-occurring with indicated crops. The taxa Fish has 4 species co-occurring with indicated crops. The taxa Insect has 

### 1 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. Kentucky

The taxa Bird has 6 species co-occurring with indicated crops. The taxa Bivalve has 22 species co-occurring with indicated crops. The taxa Crustacean has 

### 1 species co-occurring with indicated crops. The taxa Dicot has 

### 10 species co-occurring with indicated crops. The taxa Fish has 5 species co-occurring with indicated crops. The taxa Insect has 

### 1 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops. Louisiana

The taxa Bird has 4 species co-occurring with indicated crops. The taxa Bivalve has 3 species co-occurring with indicated crops. The taxa Dicot has 2 species co-occurring with indicated crops. The taxa Ferns has 1 species co-occurring with indicated crops. The taxa Fish has 2 species co-occurring with indicated crops. The taxa Mammal has 

### 1 species co-occurring with indicated crops. The taxa Marine mml has 1 species co-occurring with indicated crops. The taxa Reptile has 7 species co-occurring with indicated crops. Maine

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Dicot has 

### 1 species co-occurring with indicated crops. The taxa Fish has 2 species co-occurring with indicated crops. The taxa Mammal has 

### 1 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops.



## 68AL, AK, 

### AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, ID, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, ND, OH, OK, OR, PA, PR, RI, SC, SD, TN, TX, UT, VT, VA, WA, WV, WI, WY Maryland The taxa Bird has 1 species co-occurring with indicated crops. The taxa Bivalve has 1 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Fish has 2 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. Massachusetts

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Dicot has 1 species co-occurring with indicated crops. The taxa Fish has 1 species co-occurring with indicated crops. The taxa Insect has 3 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 2 species co-occurring with indicated crops. Michigan

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 2 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Ferns has 1 species co-occurring with indicated crops. The taxa Insect has 4 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 3 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. Minnesota The taxa Bird has 1 species co-occurring with indicated crops. The taxa Bivalve has 2 species co-occurring with indicated crops. The taxa Dicot has 2 species co-occurring with indicated crops.



## 69AL, AK, 

### AZ.., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY The taxa Fish has 

### 1 species co-occurring with indicated crops. The taxa Insect has 

### 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. 

### Mississippi

The taxa Amphibian has 

### 1 species co-occurring with indicated crops. The taxa Bird has 4 species co-occurring with indicated crops. The taxa Bivalve has 9 species co-occurring with indicated crops. The taxa Dicot has 2 species co-occurring with indicated crops. The taxa Ferns has 1 species co-occurring with indicated crops. The taxa Fish has 3 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops. The taxa Reptile has 7 species co-occurring with indicated crops. 

### Missouri

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 6 species co-occurring with indicated crops. The taxa Crustacean has 

### 1 species co-occurring with indicated crops. The taxa Dicot has 7 species co-occurring with indicated crops. The taxa Fish has 7 species co-occurring with indicated crops. The taxa Gastropod has 

### 1 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. 

### Montana

The taxa Bird has 3 species co-occurring with indicated crops. The taxa Dicot has 2 species co-occurring with indicated crops. The taxa Fish has 4 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops.



### 70AL, AK, 

### AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, ID, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, ND, OH, OK, OR, PA, PR, RI, SC, SD, TN, TX, UT, 

### VT, VA, 

### WA,WV, WI, WY

Nebraska

The taxa Bird has 3 species co-occurring with indicated crops. The taxa Dicot has 2 species co-occurring with indicated crops. The taxa Fish has 2 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. Nevada

The taxa Dicot has 8 species co-occurring with indicated crops. The taxa Fish has 16 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. NewHampshire

The taxa Bivalve has 1 species co-occurring with indicated crops. The taxa Dicot has 1 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. 

### NewJersey

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Dicot has 2 species co-occurring with indicated crops. The taxa Fish has 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 3 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. 

### NewMexico

The taxa Amphibian has 1 species co-occurring with indicated crops. The taxa Bird has 6 species co-occurring with indicated crops.



### 71The taxa Crustacean has 2 species co-occurring with indicated crops.

AL, AK, 

### AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY The taxa Dicot has 11 species co-occurring with indicated crops. The taxa Fish has 13 species co-occurring with indicated crops. The taxa Gastropod has 5 species co-occurring with indicated crops. The taxa Mammal has 4 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. 

### New York

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 1 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Ferns has 1 species co-occurring with indicated crops. The taxa Fish has 1 species co-occurring with indicated crops. The taxa Gastropod has 1 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. 

### North Carolina

The taxa Arachnid has 1 species co-occurring with indicated crops. The taxa Bird has 4 species co-occurring with indicated crops. The taxa Bivalve has 8 species co-occurring with indicated crops. The taxa Dicot has 21 species co-occurring with indicated crops. The taxa Fish has 4 species co-occurring with indicated crops. The taxa Gastropod has 1 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Lichen has 1 species co-occurring with indicated crops. The taxa Mammal has 4 species co-occurring with indicated crops. The taxa Marine mml has 1 species co-occurring with indicated crops. The taxa Monocot has 5 species co-occurring with indicated crops.



### 72The taxa Reptile has 5 species co-occurring with indicated crops.

AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY

North Dakota

The taxa Bird has 3 species co-occurring with indicated crops. The taxa Fish has 1 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. Ohio

The taxa Bird has 1 species co-occurring with indicated crops. The taxa Bivalve has 6 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Fish has 1 species co-occurring with indicated crops. The taxa Insect has 4 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 2 species co-occurring with indicated crops. Oklahoma

The taxa Bird has 6 species co-occurring with indicated crops. The taxa Bivalve has 2 species co-occurring with indicated crops. The taxa Fish has 4 species co-occurring with indicated crops. The taxa Insect has 1 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. Oregon

The taxa Bird has 3 species co-occurring with indicated crops. The taxa Crustacean has 1 species co-occurring with indicated crops. The taxa Dicot has 9 species co-occurring with indicated crops. The taxa Fish has 22 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops.



### 73

## 74AL, AK, 

### AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MO, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY
Pennsylvania The taxa Bird has 

### 1 species co-occurring with indicated crops. The taxa Bivalve has 2 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 

### 1 species co-occurring with indicated crops. Puerto Rico

The taxa Amphibian has 3 species co-occurring with indicated crops. The taxa Bird has 6 species co-occurring with indicated crops. The taxa Oicot has 28 species co-occurring with indicated crops. The taxa Ferns has 7 species co-occurring with indicated crops. The taxa Marine mml has 1 species co-occurring with indicated crops. The taxa Monocot has 4 species co-occurring with indicated crops. The taxa Reptile has 7 species co-occurring with indicated crops. Rhode Island

The taxa Bird has 1 species co-occurring with indicated crops. The taxa Oicot has 

### 1 species co-occurring with indicated crops. The taxa Fish has 

### 1 species co-occurring with indicated crops. The taxa Insect has 

### 1 species co-occurring with indicated crops. The taxa Mammal has 

### 1 species co-occurring with indicated crops. The taxa Monocot has 

### 1 species co-occurring with indicated crops. South Carolina

The taxa Amphibian has 1 species co-occurring with indicated crops. The taxa Bird has 4 species co-occurring with indicated crops. The taxa Bivalve has 

### 1 species co-occurring with indicated crops. The taxa Oicot has 

### 12 species co-occurring with indicated crops. The taxa Ferns has 1 species co-occurring with indicated crops. The taxa Fish has 

### 1 species co-occurring with indicated crops. The taxa Lichen has 

### 1 species co-occurring with indicated crops.



### 75The taxa Mammal has 1 species co-occurring with indicated crops.



## 76AL, AK, 

### AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT, VA, WA, WV, WI, WY The taxa Marine mml has 3 species co-occurring with indicated crops. The taxa Monocot has 6 species co-occurring with indicated crops. The taxa Reptile has 5 species co-occurring with indicated crops. South Dakota

The taxa Bird has 3 species co-occurring with indicated crops. The taxa Fish has 2 species co-occurring with indicated crops. The taxa Insect has 

### 1 species co-occurring with indicated crops. The taxa Mammal has 

### 1 species co-occurring with indicated crops. The taxa Monocot has 

### 1 species co-occurring with indicated crops. Tennessee

The taxa Arachnid has 

### 1 species co-occurring with indicated crops. The taxa Bird has 3 species co-occurring with indicated crops. The taxa Bivalve has 38 species co-occurring with indicated crops. The taxa Crustacean has 

### 1 species co-occurring with indicated crops. The taxa Dicot has 

### 16 species co-occurring with indicated crops. The taxa Ferns has 

### 1 species co-occurring with indicated crops. The taxa Fish has 

### 16 species co-occurring with indicated crops. The taxa Gastropod has 3 species co-occurring with indicated crops. The taxa Lichen has 1 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. Texas

The taxa Amphibian has 4 species co-occurring with indicated crops. The taxa Arachnid has 

### 10 species co-occurring with indicated crops. The taxa Bird has 

### 11 species co-occurring with indicated crops. The taxa Crustacean has 

### 1 species co-occurring with indicated crops. The taxa Dicot has 26 species co-occurring with indicated crops. The taxa Fish has 7 species co-occurring with indicated crops. The taxa Gastropod has 1 species co-occurring with indicated crops.



## 77AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, ID, IL, IN, IA, KS, KY, LA, ME, MD, MA, MI, MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, ND, OH, OK, OR, PA, PR, RI, SC, SD, TN, TX, UT, VT, VA, WA, WV, WI, WY The taxa Insect has 9 species co-occurring with indicated crops. The taxa Mammal has 5 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 6 species co-occurring with indicated crops. Utah

The taxa Bird has 2 species co-occurring with indicated crops. The taxa Dicot has 19 species co-occurring with indicated crops. The taxa Fish has 8 species co-occurring with indicated crops. The taxa Mammal has 2 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops. Vermont

The taxa Bivalve has 1 species co-occurring with indicated crops. The taxa Dicot has 1 species co-occurring with indicated crops. The taxa Mammal has 1 species co-occurring with indicated crops. The taxa Monocot has 1 species co-occurring with indicated crops. Virginia

The taxa Amphibian has 1 species co-occurring with indicated crops. The taxa Bird has 2 species co-occurring with indicated crops. The taxa Bivalve has 21 species co-occurring with indicated crops. The taxa Crustacean has 2 species co-occurring with indicated crops. The taxa Dicot has 13 species co-occurring with indicated crops. The taxa Fish has 7 species co-occurring with indicated crops. The taxa Gastropod has 1 species co-occurring with indicated crops. The taxa Insect has 3 species co-occurring with indicated crops. The taxa Mammal has 4 species co-occurring with indicated crops. The taxa Monocot has 4 species co-occurring with indicated crops. The taxa Reptile has 1 species co-occurring with indicated crops.



### 78AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI, MN,MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PRo RI, SC, SO, TN, TX, UT, VT, 

### VA, WA, WV, WI, WY 

### Washington The taxa Bird has 3 species co-occurring with indicated crops. The taxa Dicot has 7 species co-occurring with indicated crops. The taxa Fish has 

### 19 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops. 

### West Virginia

The taxa Amphibian has 

### 1 species co-occurring with indicated crops. The taxa Bivalve has 5 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Gastropod has 

### 1 species co-occurring with indicated crops. The taxa Mammal has 4 species co-occurring with indicated crops. The taxa Monocot has 

### 1 species co-occurring with indicated crops. 

### Wisconsin

The taxa Bird has 3 species co-occurring with indicated crops. The taxa Bivalve has 2 species co-occurring with indicated crops. The taxa Dicot has 4 species co-occurring with indicated crops. The taxa Insect has 2 species co-occurring with indicated crops. The taxa Mammal has 

### 1 species co-occurring with indicated crops. The taxa Monocot has 2 species co-occurring with indicated crops. 

### Wyoming

The taxa Dicot has 2 species co-occurring with indicated crops. The taxa Mammal has 3 species co-occurring with indicated crops. 

### No species were excluded,

Dispersed species included in report.



## 79