FROM:

U. S. ENVIRONMENTAL PROTECTION AGENCY

Washington, D.C. 20460

OFFICE OF CHEMICAL

SAFETY AND

POLLUTION PREVENTION

Date: February 22, 2011

Chemical: Pyraclostrobin

PC Code: 099100

DP Barcodes: 380640,380650,384006,384010,384012, and 385332

Dec.t"!1I07\ : 4~:2~"1) 4~41 '05) 4311.CJO

MEMORANDUM:

SUBJECT: Ecological Risk Assessment to Support the Proposed New Use of

Pyraclostrobin on Rapeseed (Cultivars, Varieties, and/or Hybrids,

Including Canola and Crambe), Sweet Com and Sugar Beet and as an

Encapsulated Product on Field Com, Pop Com and Seed Production Com

Lewis Ross Brown, Environmental Biologist ;;i~ n r~- ,. , ,

Robert Miller, Environmental Protection Specia.list'1l:.l-l (Jr, C/h .J~. It;.,) 1 .j~60l ... l<i ~·t': s r« . '/ll"II,;' \( - oM )

THROUGH: Nancy Andrews, Branch Chief f .,:r(l-vzf 16- ~1 d:lr1 f (

Sujatha Sankula, Lead Biologist '-'Y'-\~ ,\_o-~ Environmental Risk Branch I ~;::::::::::::-='-

Environmental Fate and Effects Division (7507P)

TO: Tony Kish, Risk Manager

Shaunta Hill, Risk Manager Reviewer

Fungicide Branch

Registration Division (7505P)

1. Executive Summary

This ecological risk assessment evaluates the proposed new uses ofthe following

pyraclostrobin products, Coronet seed treatment on rapeseed (cultivars, varieties, and/or

hybrids, including canola and crambe), BAS 500 20 F on field com, pop com and seed

production com and Stamina seed treatment on sweet com and sugar beet. This risk

assessment focuses on the environmental fate and effects ofpyraclostrobin and not it's

major degradates due to the lack environmental fate and toxicity data for the major

degradates.

The proposed maximum application rates for seed treatments are calculated to be 0.0096

lbs. a,ijA for rapeseed 0.003 lbs. a,ijA for sweet com and 0.11 lbs. a.i.zA for sugar beet.

1

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2086342

TEXT SEARCHABLE DOCUMENT 2011
The maximum ground/aerial application rate for BAS 500 20 F on corn is one application

ofO.llbs. a.i./A per year.

The proposed seed treatment application rates are significantly lower than the maximum

registered application rate of 0.5 lbs. a.i./A (DP barcode: D269625). Consequently, in

contrast to the currently registered uses, the proposed seed treatments result in a

significantly reduced exposure of non-target wildlife to pyraclostrobin environmental

concentrations that are predicted to be several orders of magnitude below the Agency

LOC. Also, the proposed encapsulated foliar application rate to corn is significantly less

than the registered foliar application rate of 0.5 lbs. a.i./A for corn on the Headline SC

label (EPA Reg. No. 7969-289). Therefore, EFED has concluded that a full screening

risk assessment is not warranted. Tables 1-4 lists the LOC exceedences for the proposed

new uses of pyraclostrobin.

Table 1. Summary ofLOC Exceedencesforthe Proposed New Use of

Pyraclostrobin When Used Asan Encapsulated Formulation in Corn(Field, Pop,

and Seed-production)

Taxonomic Group

Effect Mamma Terres Terrest. Aquatic Aquatic Birds Is t. Inverts Fish Inverts Plants Plants·

Direct Effect LOC YesExceedences No Freshwater Yes-Salt- No No No fishes water Inverts

No

Indirect Effect

Exceedances

Yes Yes Yes

Yes Yes Yes No

Table 2. Summary ofLOC Exceedences for the Proposed New Use of

Pyraclostrobin When Used As a Seed-treatment oBSuear BeetSeeds

Taxonomic Group

Effect Mamma Terres Terrest. Aquatic Aquatic Birds t. Fish Is Plants Inverts Inverts Plants

Direct Effect LOC YesExceedences Yes Freshwater Yes No Yes No

No fishes

Indirect Effect

Exceedances Yes Yes Yes Yes

Yes Yes No

Table 3. Summary of LOC Exceedences for the Proposed New Use of

Pyraclostrobin When Used As a Seed-treatment on Sweet Corn Seeds

Taxonomic Group

Effect Mamma Terres Terrest. Aquatic Aquatic Birds t. Fish Is Plants Inverts Inverts Plants

Direct Effect LOC

Exceedences No No No No No No

2
No

Indirect Effect

Exceedances Yes Yes Yes Yes Yes

Yes No

Table 4. Summary ofLOC Exceedences for the Proposed New Use ofPyraclostrobin When Used as

a Seed-treatment on Canola and Carambe Seeds

Taxonomic Group

Effect Mamma Terres Terrest. Aquatic Aquatic Birds t. Fish Is Plants Inverts Inverts Plants

Direct Effect LOC

Exceedences Yes Yes No Yes Yes

No No

Indirect Effect

Exceedances

Yes Yes

Yes No Yes Yes No

Risk Summary for This Assessment:

A screening-level (Level I) risk assessment suggests that pyraclostrobin as an

encapsulated foliar treatment use on com and as a seed-treatment use on sweet com,

sugar beets, and rapeseed provided the following:

• Potential Acute risks to 20-gram birds consuming pyraclostrobin-treated canola

seeds when applied as a seed treatment to rapeseed as acute risk quotients for 20-

gram avian species were exceeded at the Acute Restricted Use and Acute Listed

Species Use risk presumption categories (RQ=0.24)

• Potential Acute risks to 20-gram birds consuming pyraclostrobin-treated sugar

beet seeds as acute risk quotients for 20-gram avian species were exceeded at the

Acute Risk Use, Acute Restricted Use, and Acute Listed Species Use risk

presumption categories (RQ=3.24)

• Potential Chronic risks to 20-gram birds consuming pyraclostrobin-treated sugarbeet seeds as chronic risk quotients for 20-gram avian species were exceeded at

the chronic Level ofConcern (RQ=I2.54)

• Potential Acute risks to I5-gram mammals consuming pyraclostrobin-treated

sugar beets seeds as acute risk quotients for 15-gram mammals were exceeded at

the Acute Restricted Use and Acute Listed Species Use risk presumption

categories (RQ=0.26)

• Potential chronic risks to I5-gram mammals ingesting pyraclostrobin-treated

canola seeds when applied as a seed-treatment to rapeseed as chronic risk

quotients were exceeded (RQ=1.72)

3
• Potential chronic risks to I5-gram mammals ingesting pyraclostrobin-treated

sugar beet seeds as chronic risk quotients for I5-gram mammals were exceeded at

the chronic levels of concern (RQ=22.93)

• Potential acute risk to freshwater(cold-water) fishes from use of pyraclostrobin as

a seed treatment to canola as acute risk quotients are exceeded at the Acute Listed

Species risk presumption category (RQ=O.061)

• Potential acute risks to salt-water invertebrates (mysid shrimps) from use of

pyraclostrobin as a seed-treatment to canola as acute risk quotients are exceeded

at the Acute Listed Species risk presumption category (RQ=O.09)

• Potential acute risk to freshwater fishes (Warm-water Bluegill sunfish and the

cold-water Rainbow trout fishes) from the use of pyraclostrobin as an

encapsulated formulation on com (field, pop, and seed production) as acute risk

quotients were exceeded at the Acute Restricted Use and Acute Listed Species

Use (rainbow trout) and at the Acute Listed Species Use (bluegill sunfishes) risk

presumption categories (Rainbow trout Acute RQ=O.II and Bluegill sunfish

Acute RQ=O.06)

• Potential acute risk to salt-water mollusks(eastern oysters) from the use of

pyraclostrobin as an encapsulated formulation on com (field, pop, and seed

production) as levels of concern were exceeded at the Acute Listed Species risk

presumption category (Eastern Oyster Acute RQ=O.054)

• Potential acute risks to salt-water invertebrates (mysid shrimp) from the use of

pyraclostrobin as an encapsulated formulation on com (field, pop, and seed

production) as levels of concern were exceeded at the Acute Restricted Use and

Acute Listed Species use risk presumption categories (Mysid shrimp Acute

RQ=O.16)

• Potential acute risks to freshwater fishes (rainbow trout) from the use of

pyraclostrobin as a seed-treatment to sugar beet seeds as acute levels of concern

were exceeded at the Acute Listed Species Use risk presumption. Also, acute

levels of concern were triggered at the Acute Listed Species Use risk presumption

category for warm-water fishes (bluegill sunfishes) and at the Acute Restricted

Use risk presumption category for cold-water fishes (rainbow trout) (Rainbow

trout acute RQ=O.IO and Bluegull sunfish Acute RQ=O.05)

• Potential acute risks to salt-water invertebrates (mysid shrimp) from the use of

pyraclostrobin as a seed-treatment to sugar beets as acute levels of concern were

exceeded at the Acute Restricted Use and Acute Listed Species Use risk

presumption categories (Acute RQ=O.13)

4
• No potential acute and/or chronic risks to avian and mammalian organisms

ingesting pyraclostrobin-treated sweet com seeds as acute and chronic risk

quotients were all less than 0.01 in value.

• No potential risks to terrestrial and/or aquatic vascular and non-vascular plants

from the use of pyraclostrobin as a seed-treatment to the seeds ofsweet com,

sugar-beets, and rapeseeds as risk quotients were below the levels of concern as

risk quotients ranged from <0.01-0.13 for aquatic plants and risk quotients for

terrestrial plants were all less than 0.1.

• No potential acute and/or chronic risk to aquatic organisms and amphibians from

the use of pyraclostrobin as a seed-treatment to the seeds ofsweet com, sugarbeets, and rapeseeds as risk quotients were <0.01.

• No potential risk to non-target terrestrial insects from the use ofpyraclostrobin as

a seed-treatment to the seeds ofsweet com, sugar-beets, and rapeseeds and as an

encapsulation product to com (field, pop, and seed-production).

Data Gaps and Uncertainties:

1. Guideline aquatic metabolism studies in soil have not been submitted.

Since the registrant did not provide the Agency with the appropriate

guideline studies for anaerobic aquatic metabolism (835.4400) and aerobic

aquatic metabolism (835.4300) anaerobic soil metabolism and aerobic soil

metabolism data was extrapolated for these studies following the "Guidance

for Selecting Input Parameters in Modeling the Environmental Fate and

Transport of Pesticides; Version 2.1, October 22,2009. Aerobic aquatic

metabolism and anaerobic aquatic metabolism data will provide a basis for a

more refined aquatic exposure assessment for future new uses.

2. Guideline estuarine/marine invertebrate lifecycle/chronic reproductive

study exposing pyraclostrobin to estuarine/marine invertebrates on a

chronic reproductive basis (850.1350) have not been submitted. There is

insufficient data to definitively characterize chronic risks to estuarine/marine

invertebrates. However, acute toxicity data suggest that estuarine/marine

invertebrates (mysids) are more sensitive to pyraclostrobin toxicity than

freshwater invertebrates. For example, the acute LCsovalues for the mysid

shrimp (Americamysis bahia) and daphnids (Daphnia magna) are 4 ppb

(MRID 45118412) and 16 ppb (MRID 45118407), respectively.

Furthermore, the relationship between acute and chronic endpoints for

freshwater invertebrates, when applied to estuarine/marine acute data,

suggests a possible chronic concern for estuarine/marine invertebrates.

Because ofthis relationship and the fact that several crop uses of

pyraclostrobin are associated with estuarine/marine habitat, a life-cycle test

for mysids (850.1350) must be submitted.

5
3. Whole Sediment acute toxicity invertebrates (freshwater) test~850.1735

have not been submitted. In the aquatic ecosystems systems, pyraclostrobin

is likely partitioned to sediments. Thus, sediment toxicity data for aquatic

invertebrates are needed in order to assess the risks to sediment dwelling

organisms. Since pyraclostrobin may potentially bind to sediment and it is

also found to be characterized on an acute basis as "very highly toxic" to

freshwater invertebrates inhabiting the water column, this study must be

submitted. Moreover, the lack ofthe study will be considered as a "Data Gap"

until the Agency receives this study.

4. Whole sediment acute toxicity invertebrates (marine) test-850.1740 have

not been submitted. As stated earlier in this assessment, pyraclostrobin may

potentially bind to sediment in aquatic environments. Since pyraclostrobin

may potentially bind to sediment and it is also found to be characterized on an

acute basis as "very highly toxic" to salt-water invertebrates inhabiting the

water column, this study must be submitted. Moreover, the lack ofthe study

will be considered as a "Data Gap" until the Agency receives this study

5. Ecotoxicological, toxicological and environmental fate data for the three

major degradates formed from the breakdown ofthe parent pyraclostrobin,

BF 500-11, BF 500-13, and BF 500-14, were only submitted for freshwater

fishes and invertebrates. No other info on the environmental fate,

ecotoxicity, and toxicity effects these degradates may exert on other aquatic

and terrestrial organisms was submitted for use in this assessment. Therefore,

the environmental fate ofthese degradates in the environment as well as

overall the ecotoxicity and toxicity effects these degradates may pose

terrestrial organisms (either directly or indirectly) are unknown at this time.

6. The use ofthe acute to chronic ratio to estimate a No Observable Adverse

Effect Concentration (NOAEC) value for estuarine/marine invertebrates.

This method usually introduces a high level of uncertainty into the overall

risk estimation and risk conclusions as the risks may have been over or

under-estimated.

7. This assessment does not analyses other possible routes of exposure such as

inhalation and accidental ingestion of soil sediments to which pyraclostrobin

could bind in terms ofterrestrial and aquatic organisms being exposed.

2. Scope of This Assessment

An abbreviated review was conducted by EFED for the following reasons.

• No new data have been submitted that affect the environmental fate or effects

conclusions or modeling inputs;

• Proposed application rates, intervals, and seasonal loads are less than previously

approved application rates;

6
• No new exposure methods have been approved for standard risk assessments

since the last chemical assessment was performed in 2010.

3. Findings of Prior Risk Assessments

Previous pyraclostrobin risk assessments by EFED indicate the following direct and indirect effects in terrestrial and aquatic organisms:

• Mammals and aquatic organisms are subject to adverse direct effects such as reduced

survival, growth, and reproduction when exposed to pyraclostrobin residues as a

result oflabeled use ofthe pesticide. Birds are not subject to adverse direct effects

such as reduced survival, growth, and reproduction when exposed to pyraclostrobin

residues as a result oflabeled use ofthe pesticide.

• Non-target terrestrial, semi-aquatic, and aquatic plants are not subject to adverse

effects such as reductions in vegetative vigor and seedling emergence (terrestrial) or

biomass and growth rate (aquatic) when exposed to pyraclostrobin residues as a result

oflabeled use ofthe pesticide.

• Indirect effects, such as food web dynamics, perturbing forage or prey availability,

and altering the extent and nature of nesting, will potentially occur as residue

concentrations exceeded levels of concern for acute or chronic exposure for terrestrial

and/or aquatic species.

• Listed species are subject to adverse effects as the calculated risk quotients exceeded

Acute Listed or Chronic Levels ofConcern.

4. Environmental Fate Summary

Pyraclostrobin (cabamic acid, [2-[[[1-(4-chlorophenyl)-1H-pyrazol-3-

yl[oxy]methyljphenyl]methoxy-,methyl ester) belongs to the strobilurin family of

fungicides. The mode of action of pyraclostrobin is believed to be the inhibition of

mitochondrial respiration in fungal cells. Pyraclostrobin, like other strobilurin

fungicides, also inhibits spore germination and is reported as a powerful antisporulant.

Although strobilurins do not have systemic activity, they exhibit unique translaminar

activity, i.e., the fungicide could move from the sprayed side to the unsprayed side of a

given leaf. In this context, they are classified as 'surface systemic' or 'mesosystemic'

fungicides. Figure 1 displays the molecular structure of pyraclostrobin.

(') Cr-Q-N(YO~ - - /0I(N CH3 H C ' " 3 0

°

7
Figure 1: Molecular Structure of Pyraclostrobin

A major route of dissipation for pyraclostrobin is runoff. Two main factors contribute

towards pyraclostrobin ability to contaminate surface water via runoff. First,

pyraclostrobin is practically immobile with Kocs between 6,000 mg/L and 16,000 mg/L in

all soil studies with an arithmetic mean of9,304 mg/L and median of7,889 mg/L,

Second, laboratory studies suggest that pyraclostrobin is moderately persistent in aerobic

soil environments (t1/2 = 81.5 days). Adsorption/desorption studies suggest that

pyraclostrobin has a tendency to adsorb to the soil particles. Laboratory aerobic soil

metabolism studies are supported by field dissipation studies findings suggesting that

pyraclostrobin is moderately persistent in soil.

Aqueous photolysis (T1/2 = 0.08 days) and anaerobic soil metabolism (t1/2 = 3.0 days)

appear to be the main routes of degradation. Pyraclostrobin is considered to be stable to

hydrolysis and soil photolysis. Fish bioaccumulation data suggest, Log Kow 4.18 at pH

6.5 that pyraclostrobin has a tendency to bioaccumulate in fish; however, depuration is

rapid.

5. Summary of Proposed Uses

The proposed new use for Coronet (9.0% a.i.) seed treatment on rapeseed allows for a

maximum application of 0.12 lbs a.i. per 100 lbs. ofseed. The maximum rapeseed

(cultivars, varieties, and/or hybrids, including canola and crambe) seeding rate is 8 lbs of

seed per acre (http://www.ipmcenters.org/cropprofiles/CP form.cfm); therefore, the

maximum application rate for rapeseed is 0.0096 lbs. a.i.lA. The new uses and proposed

application rates and methods are listed in Table 5.

The proposed increased use rate for Stamina (18.4% ai) seed treatment on sweet com

permits a maximum application of 0.02 lbs. a.i. per 100 lbs ofseed. The maximum

seeding rate for sweet com is 15 lbs a.i.lA (http://nwrec.hort.oregonstate.edulcornfr.html); therefore, the maximum application rate for Stamina on sweet com is 0.003 lbs.

a.i.lacre.

The proposed decreased use rate for Stamina on sugar beet permits a maximum of 0.033

lbs. a.i. per 100,000 seeds (40,000 seeds per pound,

http://sugarbeet.ucdaviseduinoteslJune98.html) at a maximum seeding rate of 8 lbs.

(http://www.agecon ucdavis.eduioutreachicrop/Archives/SugarBeets.pdf) per acre,

therefore, the maximum application rate for Stamina on sugar beet is 0.1056 lbs. a.i.lacre.

The maximum application rate for BAS 50020 F (23.06 %) is one application ofO.llbs.

a.i.lA per year. BAS 500 20 F is a new encapsulated product comparable in its proposed

use pattern and percentage active ingredient to Headline® Fungicide (23.6 a.i.lA)(EPA

Reg. No. 7969- 186). BASF submitted "The Wash-off ofEncapsulated Pyraclostrobin

(BAS 500 F) from Com Plants" non-guideline study (MRID # 48037313 ) for the

registration ofBAS 500 20 F Fungicide to address the dissipation/release rate ofthe

active ingredient from the wash-off of encapsulated material that will be deposited onto

soil. This study was reviewed and classified ancillary information.

8
BASF also submitted "Comparison of Pyraclostrobin (BAS 500 F) Field Soil Dissipation

Endpoints from North America with EC, WG, and CS Formulations" paper (MRID #

48037314) to demonstrate that formulation type is not an important factor influencing the

field dissipation of pyraclostrobin. This study was reviewed and classified ancillary

information.

Table 5. Pyraclostrobin Application Rates for BAS 500 20 F, Coronet and Stamina

Formulations

Formulation Crops Max. Max. #of Type of

Application Rate Applications Application

lbs. a.i./Acre

BAS 50020 F Field com, pop com and seed 0.10 I Foliar

[production com (Aerial/Ground)

Coronet Rapeseed (cultivars, varieties,

and/or hybrids, including 0.0096 1 Seed treatment

canola and crambe)

Stamina Sweet com 0.0034 1 Seed treatment

Sugar beet 0.1056 1 Seed treatment

Table At in Appendix A shows a listing of crops already registered for pyraclostrobin

use.

6. Exposure Summary

6.1 TerrestrialExposures

Consumption of pyraclostrobin on contaminated foliage, soil or seeds are the most likely

exposure route for terrestrial animals. The Terrestrial Exposure (TREX) Model (Version

1.4.1) was used to estimate the dietary exposure to terrestrial birds and mammals. Typical

input values (for foliar treatments) for TREX include application rate, time intervals

between applications, total number of applications, foliar half-life periods, and toxicity

data. TREX modeled input parameters and outputs are summarized in Appendix E.

Bird and Mammal Exposure Assessment

Estimated exposure concentrations for terrestrial receptors were determined using the

standard screening-level exposure model, TREX (v. 1.4.1.) (EPA, December 7,2008).

Essentially, for a single application, there is a linear relationship between the amount of

pesticide applied and the amount of pesticide residue present on a given food item. These

relationships for the various food items are determined from the Kenaga nomogram as

modified by Fletcher (Hoerger and Kenaga, 1972; Fletcher et aI., 1994). TREX (v.1.4.1)

is a terrestrial exposure model that, in addition to incorporating the nomogram

relationship, also includes pesticide degradation in the estimate ofEECs.

Foliar dissipation half-lives are incorporated into the TREX model. The foliar dissipation

half-life default of 35 days was used to provide an upper-bound pyraclostrobin residue

concentration on foliage due to no foliar residue dissipation data being available from the

9
Agency's Human Effect Division (HED). Maximum Kenaga dose-based and dietary

EECs for a single encapsulated spray application (aerial and/or ground) ofpyraclostrobin

(BAS 500 20F) to com using the recommended label rate for birds are shown in 6 and 7

while the maximum Kenaga dose-based EECs for mammals is presented in Tables 8 and

9. EECs for seed treatment uses are presented in Table 10.

Table 6. Estimates of Avian Dietary Based-maximum Kenaga EECs for Foliar Residues I of Pyraclostrobin on Corn (Field, Pop, and Seed-production) with a Foliar Dissipation

tl/2 = 35 day Half-life Value and a Single Application

UsefApp. Application Rate Ibs. a.i.fA Food Items MaximumEEC

Method (# app f interval, days) (mglkg)

Com (field, pop, Short grass 24,00

I

and seedproduction) 0,10 Ibs ai/A with only I Tall grass 11.00

(Encapsulated application per growing season

Spray Aerial per year Broadleaf plants/small insects 13,5 -

and/or ground

I

Fruits, pods, seeds, and large insects 1.50

applications) I ....-J

Table 7. Estimates ofAvian Dose-Based Maximum Kenaga EECs for use ofDAS 500 20F

on Com (Field, Pop, and Seed-production) with a Foliar Dissipation tl/2 = 35 Day Half-life

Value and a Single Application

UsefApp. Application Food Items 20 grams 10o-grams lOOO-grams

Method Rate Ibs.a.iJA

(# app f interval,

davsf

Com (field, pop, 0,10 Ibs aifA with Short Grass 27.33 15,59 6,98

and seed- only I

production) application per

(Encapsulated growing season

Spray Aerial per year

and/or ground

applications)

Tall grass 12,53 7,14 320

Broadleaf plants/small

insects 15,38 8,77 3,93

Fruits/pods/seeds/large

insects 1.71 0,97 0.44

Granivores 0.38 0.22 0,10

Table 8. Es"'tes of MI......

588.Fo. €onl(l'iet8,. 1-l.....iIId

HaIf..Iife"VaIue aada,Single~'

Usd4pp.

Method

Com (field, pop,

and seedproduction)

(Encapsulated

Spray Aerial

and/or ground

a lications)

.Rate ibi~a.iJA ,'~"

(# app I iatervaI,

dlts

0, I0 Ibs ailA with Short Grass

only I

application per

growing season

per year

10

15gi__

22,88 15,81 367
Tall grass 10.49 7.25 1.68

Broadleaf plants/small

insects 12.87 8.90 2.06

Fruits/pods/seeds/large

insects 1.43 0.99 0.23

Granivores 0.32 0.22 0.05

Table 9. Estimates of Mammalian Dietary Based-maximum Kenaga EECs for Foliar

Residues of Pyraclostrobin on Corn (field, pop, and seed-production) with a Foliar

Dissipation till =35 Days Half-life Value and a Single Application

UseiApp. Application Rate Ibs. a.iJA Food Items MaximumEEC

Method (# app / interval, days) (mg/kg)

Corn (field, pop, Short grass 24.00

and seed- 0.10 Ibs ai/A with only I production) Tall grass 11.00

application per growing season (Encapsulated per year Broadleaf plants/small insects 13.5 Spray Aerial

and/or ground Fruits, pods, seeds, and large insects 1.50

applications)

For seed-treatment, T-REX seed-treatment model (v.1.4.1) was used to estimate avian

and mammalian EECs for terrestrial organisms exposed to pyraclostrobin-treated sweetcorn seeds. These EECs are presented in Table 10 ofthis document. The EECs are

expressed as Exposed avianlNAGY Dose (mg ai/kg-bw/day) and

marnmalianlNAGYDose (mg ai/kg-bw/day) for the soil incorporation ofsweet corn, ,

canola seeds, and sugar beet seeds

Table 10. Seed Treatment EECs for Pyraclostrobin Applied As a Seed Treatment

for Sweet Corn, Canola and SU2ar Beet Seeds

Maximum Maximum Seed Avian Nagy Dose Mammalian Nagy Available ai

Application rate Application Rate (mg ai/kg-bw/day) (mg ai/kg-bw/day) (mg ai ft -2)

(lbs ai/a) (mg ai/kg seed)

Sweet com 0.003 200.00 50.62 42.37 0.03

Sugarbeet 0.11 13,300.00 3366.08 2817.90 1.11

Rapeseed 0.01 1000.00 253.09 211.87 0.08

5.2 Aquatic Exposures

5.2.1 Input Parameters

The maximum application rates for Coronet, BAS 50020 F and Stamina input into

PRZMlEXAMS are listed in Table 5 on page 9. PRZM/EXAMS input parameters for

pyraclostrobin are shown in Appendix B and example PRZMlEXAM output files are

displayed in Appendix C.

5.2.2 Aquatic Exposure Results

The MS corn scenario generated the highest peak. (0.68 ug/L) and 60 day EEC

(G.Ilug/L) and the NC corn ground scenario produced the highest 21-day (0.19 ug/L)

averages. Results ofthe PRZM/EXAMS modeling are displayed in Table 11.

11
Table 11. Estimated Environmental Concentrations of Pyraclostrobin

Crop Scenarios Peak 21day Cone. 60 day Cone.

Cone.(uz/L) (uz/L) (ul!!L)

Coronet (seed treatment) (0.012 1bs. a.i./acre)

ND Canola 0.38 0.11 0.06

BAS 500 20 F (l aerial application at O.llbs ai/acre)

MSCorn 0.68 0.18 0.11

NCCorn 0.60 0.19 0.09

ILCorn 0.39 0.17 0.10

Stamina (seed treatment)

Corn (0.003 lbs. a.i./acre)

MS Sweet Com 0.02 0.007 0.003

NC Sweet Com 0.005 0.001 0.0008

IL Sweet Com 0.01 0.004 0.002

SU2ar Beet (0.104Ibs. a.i./acre)

MN Sugar Beet 0.56 0.18 0.06

5. Ecological Effects Summary

Based on the aquatic toxicity studies, the toxicity endpoints used for the aquatic risk

assessment of pyraclostrobin are characterized as follows.

•

•

•

•

•

•

•

•

•

•

•

Fish (freshwater) acute: Very highly toxic (LCso = 6.2 ppb)

Fish (freshwater) chronic: (NOAEC = 2.35 ppb)

Fish (estuarine/marine) acute: Very highly toxic (LCso = 76.9 ppb)

Fish (estuarine/marine) chronic: (NOAEC = 10.8 ppb)

Invertebrate (freshwater) acute: Very highly toxic (ECso = 15.7 ppb)

Invertebrate (freshwater) chronic: (NOAEC = 4.0 ppb)

Invertebrate (estuarine/marine) acute: Very highly toxic (LCso= 4.16 ppb)

Invertebrate (estuarine/marine) chronic: No data ACR used to estimate NOAEC

Vascular plants (freshwater, Non-endangered) acute: (ECso= 1720 ppb)

Vascular plants (freshwater, Endangered) acute: (NOAEC = 896 ppb)

Non Vascular Plants (freshwater) acute: (ECso= 1.5 ppb)

Non Vascular Plants (estuarine/marine) acute: (ECso = 64.7 ppb)

Based on the terrestrial toxicity studies, the toxicity endpoints used for the terrestrial risk

assessment of pyraclostrobin are characterized as follows.

•

•

•

Avian acute oral: Practically non-toxic (LDso : >2000 mg a.i.lKg BW/day)

Avian acute dietary: Practically non-toxic (LCso : >5000 mg a.i.lKg diet or PPM)

Avian reproduction: (None ofthe endpoints affected up to the highest dose tested;

NOAEC: 1062 mg a.i.lKg diet or PPM)

12
• Mammalian acute oral:(LDso : >5000 mg a.i.lKg BW for males and females)

• Mammalian chronic reproduction: (Prenatal developmental toxicity study in

rabbits; fetal resorptions and post implantation loss, NOAEL: 5 mg a.i.lKg

BW/day)

• Mammalian chronic reproduction(NOAEL= up to 300 mg/kg-diet in mammals)

• Honey bee acute: Practically nontoxic (LDso :>100 ug a.i./bee)

• Seedling Emergence, Tier I (Monocots and Dicots) (EC2s 21 days (Monocots and

Dicots): > 0.118 Ib a.i. /acre (percentemergence, percentsurvival, phytotoxicity

rating, plant heightand dry weight)NOAEC : 0.118 Ib a.i. /acre

• Vegetative Vigor Tier I (Monocots and Dicots) EC2s 21 days (Monocots and

Dicots): > 0.1181b a.i. /acre (Same parameters as in Emergence) NOAEC :

0.118 Ib a.i. /acre

• (Same parameters as in Emergence)

Full listing ofthe toxicity and ecotoxicity data for pyraclostrobin exposure to terrestrial

and aquatic organisms and plants is available in Appendix G ofthis assessment.

6. Ecological Risks Summary

A full summary ofthe potential risks identified in this assessment is presented in the

subsequent sections. A complete list of aquatic organism risk quotients (acute and

chronic) are presented in Appendix D. Tables 11-14 show the acute and chronic risk

quotients ofthe most sensitive aquatic organisms for exceedence ofthe LOC did occur:

Table 11. Aquatic Organism Risk Quotients of STAMINA to Sugar Beets Seeds

(O.104Ibs. a.iJacre)

Aquatic Acute Chronic Peak 21- 60- Acut Chroni LOC

Organisms Toxicit Toxicity EEC day Day eRQ cRQ Exceedenc

yValue Value (flg/L EEC EEC e

(flg/L) (flg/L) ) {flfL (flg/L

)

Rainbow Trout LCso 96 N/A 0.56 0.18 0.06 0.10 Acute

(Oncorhynchu hr: 6.2 Restricted

s mykiss- Use LOC is

Warm-water triggered

fish) and Acute

Listed

Species Use

is exceeded

Fathead N/A NOAEC=4.14 0.56 0.18 0.06 0.01 None

Minnow

(Pimephales

promelasy

Rainbow Trout N/A NOAEC=2.35 0.56 0.18 0.06 0.03 None

(Oncorhynchu

s mykiss)

Sheepshead LCso 96 NOAEC=10.8 0.56 0.18 0.06 <0.01 <0.01 None

Minnow hr: 76.9

(Cyprinodon

variegates13
Estuanne/manne rnysid Chronic NOAEC Value was estimated usmg the ACR method and the value may be over or

underestimated.

Salt-Water

Fish)

Daphnia ECso 48 0.56 0.18 0.06 0.04 None

magna hr: 15.7

a.i.

(14.2-

17.4)

ug/L

Daphnia NOAEC: 41lg 0.56 0.18 0.06 0.05 None

magna a.i. /L

Eastern Oyster ECso 96 0.56 0.18 0.06 0.04 None

hr: 12.5 (Crassostrea a.i.(9.2- virginica) 17.1) Mollusk Shell Ilg/L Deposition

Mysid Shrimp r.c., 96 0.56 0.18 0.06 0.13 Acute

hr:4.16 Restricted (Americamysis Use and bahia) Acute

Listed

Species

LOCs are

exceeded

Mysid Shrimp NOAEC=I.06 0.56 0.18 0.06 0.17 None

(Americamysis I

bahia)

1.

Table 12. Aquatic Organism Risk Quotients ofBAS SOO 20 F (1 Application at 0.1

Ibs. a.i.lA Applied to Com (Field, Pop, and Seed Production)

Aquatic Acute ChronicToxicity Peak 21-day 6O-Day Acute Chronic LOC

Organisms Toxicity . Value EEC EEC EEC RQ RQ Exeeedenee

Value (11g!L) (11g!L) (11g!L) (11g!L)

(11g!L)

Rainbow Trout LCso 96 N/A 0.68 0.18 0.11 0.11 Yes

(Oncorhynchus hr: 6.2 Acute

mykiss-Warm- Restricted

water fish) Use and

Acute

Listed

Species

OC

exceeded

Fathead N/A NOAEC=4.14 0.68 0.18 0.11 0.03 None

Minnow

(Pimephales

promelas)

Rainbow Trout N/A NOAEC=2.35 0.68 0.18 0.11 0.05 None

(Oncorhynchus

mykiss)

14
Estuarme/marme mysid Chrome NOAEC Value was estimated usmg the ACR method and the value

may be over or underestimated.

Sheepshead LCso 96 NOAEC=10.8 0.68 0.18 0.11 <0.01 0.01 None

Minnow hr: 76.9

(Cyprinodon

variegatesSalt-Water

Fish)

Daphnia ECso48 0.68 0.18 0.11 0.043 None

magna hr: 15.7

a.i.

(14.2-

17.4)

flg/L

Daphnia NOAEC: 4 ug 0.68 0.18 0.11 0.05 None

magna a.i. /L

Eastern Oyster ECso 96 0.68 0.18 0.11 0.054 Acute

hr: 12.5 Listed (Crassostrea a.i.(9.2- Species virginica)

Mollusk Shell 17.1) LOC

Deposition flg/L exceeded

Mysid Shrimp LCso 96 0.68 0.18 0.11 0.20 Acute

hr:4.16 Restricted (Americamysis Use and bahia) Acute

Listed

Species

LOCs are

exceeded

Mysid Shrimp NOAEC=I.06 0.68 0.18 0.11 0.20 None

(Americamysis

bahia)

I.

Table 13-Coronet (seed treatment to Canola and Crambe O.0121bs ai/acre) and

Aquatic Risk Quotients

Aquatic Acute Chronic Peak 21- 60- Acut Chroni LOC

Organisms Toxicity Toxicity EEC day Day eRQ cRQ Exceedenc

Value Value (flg/L EEC EEC e

(flg/L) (flg/L) ) (ug/L (ug/l,

) )

Bluegill LCso 96 N/A 0.38 0.11 0.06 0.03 N/A None

Sunfish hr: 11.4

(Lepomis

macrochirusColdwater

fish)

Rainbow Trout r.c., 96 N/A 0.38 0.11 0.06 0.061 Yes Listed

(Oncorhynchu hr: 6.2 Species OC

s mykiss- exceeded

Warm-water

15
fish)

Fathead N/A NOAEC=4.14 0.38 0.11 0.06 0.01 None

Minnow

(Pimephales

promelas)

Rainbow Trout N/A NOAEC=2.35 0.38 0.11 0.06 0.03 None

(Oncorhynchu

s mykiss)

Sheepshead LCso 96 NOAEC=10.8 0.38 0.11 0.06 <0.01 <0.01 None

Minnow hr: 76.9

(Cyprinodon

variegatesSalt-Water

Fish)

Daphnia ECso 48 0.38 0.11 0.06 0.024 NO

magna hr : 15.7

a.i.

(14.2-

17.4)

llg/L

Daphnia NOAEC: 411g 0.38 0.11 0.06 0.028 None

magna a.i. IL

Eastern Oyster ECso 96 0.38 0.11 0.06 0.034 None

hr: 12.5 (Crassostrea a.i.(9.2- virginica) 17.1) Mollusk Shell

Deposition llg/L

Mysid Shrimp t.c., 96 0.38 0.11 0.06 0.09 Yes Acute

hr: 4.16 Listed (Americamysis Species Use bahia) LOCs are

exceeded

Mysid Shrimp NOAEC=1.06 0.38 0.11 0.06 0.10 None

(Americamys is 1

bahia)

16
Tablel4- Shows the Acute and Chronic Risk Quotients for Terrestrial Organisms Consuming

Seed-erops Treated with Pyraclostrobin. LOCs Appear in Bold: ,

Crop Maximum Maximum Avian Mammalian Available Avian Avian Mammalian Mammalian

Application Seed Nagy Nagy(mg ai 20 Chronic 15 gram 15 gram

rate Application Dose ai/kg- (mg ai ft gram Acute I chronic

(lbs ai/a) Rate (mg bw/day) -2) Acute

(mgailkg ai/kgseed) bw/dav)

Sweet 0.003 200.00 50.62 42.37 0.03 0.05 0.19 <0.01 0.34

com

Sugarbeet 0.11 13,300.00 3366.08 2817.90 1.11 3.241 12.523 0.262 22.93 4

Rapeseed 0.01 1000.00 253.09 211.87 0.08 0.245 0.94 0.02 1.726

Avian acute levels of concern are exceeded for 20-gram birds consuming sugar beet seeds treated WIth

pyraclostrobin at the Acute Risk, Acute restricted Use, and Acute Listed Species Risk presumption

categories

2. Mammalian acute levels of concern are exceeded for IS-gram mammals consuming sugar beet seeds

treated with pyraclostrobin at the Acute Restricted Use and Acute Listed Species Risk presumption

categories

3. Avian chronic levels of concern are exceeded for 20-gram birds consuming sugar beet seeds treated with

pyraclostrobin (RQ~I for exceedence to occur)

4. Mammalian chronic levels of concern are exceeded for IS-gram mammals consuming sugar beet seeds

treated with pyraclostrobin (RQ~I for exceedence to occur)

5. Avian acute levels of concern exceeded for 20-gram birds consuming rapeseed seeds treated with

pyraclostrobin at the Acute Restricted Use and Acute Listed Species Use risk presumption categories

6. Mammalian chronic levels of concern are exceeded for IS-gram mammals consuming rapeseed seeds

treated with pyraclostrobin (RQ~1 for exceedence to occur)

Terrestrial plant risk quotients were for all crops modeled in this assessment did not

trigger or exceedence any levels of concern. Terrestrial plant risk quotients ranged from

<0.1-0.13 for all crops modeled. For more details please see Appendix F

7. Endangered and Threatened Species

Tables 15-18 summarizes the conclusions of potential concerns for direct and indirect

effects to federally-listed threatened and endangered species (listed species).

Table 15. Listed Species Risks Associated With Direct or Indirect Effects Due to

Pyraclostrobin being used as an encapsulated formulation on corn (seeds, field, and

pop).

Listed Taxonomy Direct Effects Indirect Effects

Terrestrial and semi-aquatic No Yesa

plants - monocots

Terrestrial and semi-aquatic No Yesa

plants - dicots

Terrestrial invertebrates No No

Birds No Yes c

Terrestrial-phase amphibians Yes b Yes c

Reptiles Yesb Yesc

Mammals Yes Yes"

Aquatic vascular plants No No

17
Table 15. Listed Species Risks Associated With Direct or Indirect Effects Due to

Pyraclostrobin being used as an encapsulated formulation on corn (seeds, field, and

pop).

Listed Taxonomy Direct Effects Indirect Effects

Aquatic non-vascular plants a No No

Freshwater fish Yes Yes

Aquatic-phase amphibians YesD Yesc

Freshwater Invertebrates Yes Yesc

Mollusks Yes Yesc

Estuarine/marine Inverts Yes Yesc

Marine/estuarine fish Yes Yesc

a At the date ofthis publication, there are no listed aquatic non-vascular plant species. The taxonomic

group is included here for the purposes of evaluating potential contributions to indirect effects to other

taxonomy and as a record of exceedances should future listings of non-vascular aquatic plants warrant

additional evaluation of Federal actions.

b Terrestrial-phase amphibians and reptiles estimated using birds as surrogates. Aquatic-phase amphibians

estimated using freshwater fish as surrogates.

C Animals that feed on aquatic organisms may be indirectly affected.

d Indirect effects may be caused by plants that rely on fungus for growth or reproduction or that rely on

mammals as pollinators or seed dispersers.

Table 16. Listed Species Risks Associated With Direct or Indirect Effects Due to

Pyraclostrobin Being Used as a Seed-treatment to SU2ar Beet Seeds

Listed Taxonomy Direct Effects Indirect Effects

Terrestrial and semi-aquatic No Yesd

plants - monocots

Terrestrial and semi-aquatic No Yeso

plants - dicots

Terrestrial invertebrates No Yes

Birds Yes Yesc

Terrestrial-phase amphibians YesD Yes c

Reptiles NoD Yesc

Mammals Yes Yesc

Aquatic vascular plants No No

Aquatic non-vascular plants a No No

Freshwater fish Yes Yes

Aquatic-phase amphibians Yes Yesc

Freshwater Invertebrates No Yesc

Mollusks No Yesc

Estuarine/marine inverts Yes Yes

Marine/estuarine fish No Yesc

a At the date ofthis publication, there are no listed aquatic non-vascular plant species. The taxonomic

group is included here for the purposes of evaluating potential contributions to indirect effects to other

taxonomy and as a record of exceedances should future listings of non-vascular aquatic plants warrant

additional evaluation of Federal actions.

b Terrestrial-phase amphibians and reptiles estimated using birds as surrogates. Aquatic-phase amphibians

estimated using freshwater fish as surrogates.

C Animals that feed on aquatic organisms may be indirectly affected.

d Indirect effects may be caused by plants that rely on fungus for growth or reproduction or that rely on

mammals as pollinators or seed dispersers.

18
Table 17. Listed Species Risks Associated With Direct or Indirect Effects Due to

Pyraclostrobin Beine:Used As a Seed-treatment to Sweet Corn Seeds

Listed Taxonomy Direct Effects Indirect Effects

Terrestrial and semi-aquatic No YesG

plants - monocots

Terrestrial and semi-aquatic No YesG

plants - dicots

Terrestrial invertebrates No No

Birds No Yesc

Terrestrial-phase amphibians Nob Yes c

Reptiles Nob Yesc

Mammals No Yesc

Aquatic vascular plants No No

Aquatic non-vascular plants a No No

Freshwater fish No Yes

Aquatic-phase amphibians No Yesc

Freshwater Invertebrates No Yesc

Mollusks No Yesc

Estuarine/marine Inverts No Yesc

Marine/estuarine fish No Yesc

a At the date ofthis publication, there are no listed aquatic non-vascular plant species. The taxonomic

group is included here for the purposes of evaluating potential contributions to indirect effects to other

taxonomy and as a record of exceedances should future listings of non-vascular aquatic plants warrant

additional evaluation of Federal actions.

b Terrestrial-phase amphibians and reptiles estimated using birds as surrogates. Aquatic-phase amphibians

estimated using freshwater fish as surrogates.

C Animals that feed on aquatic organisms may be indirectly affected.

d Indirect effects may be caused by plants that rely on fungus for growth or reproduction or that rely on

mammals as pollinators or seed dispersers.

Table 18. Listed Species Risks Associated With Direct or Indirect Effects Due to

Pyraclostrobin Being Used as a Seed-treatment to Canola seeds

Listed Taxonomy Direct Effects Indirect Effects

Terrestrial and semi-aquatic No YesG

plants - monocots

Terrestrial and semi-aquatic No YesG

plants - dicots

Terrestrial invertebrates No No

Birds Yes Yesc

Terrestrial-phase amphibians Yes Yes c

Reptiles No Yesc

Mammals No Yesc

Aquatic vascular plants No Yesc

Aquatic non-vascular plants a No Yesc

Freshwater fish Yes Yes

Aquatic-phase amphibians No Yesc

Freshwater Invertebrates No Yesc

Mollusks No Yesc

Estuarine/marine inverts Yes Yesc

Marine/estuarine fish No Yesc

19
Table 18. Listed Species Risks Associated With Direct or Indirect Effects Due to

Pyraclostrobin Being Used as a Seed-treatment to Canola seeds

Listed Taxonomy I Direct Effects I Indirect Effects

a At the date ofthis publication, there are no listed aquatic non-vascular plant species. The taxonomic

group is included here for the purposes of evaluating potential contributions to indirect effects to other

taxonomy and as a record of exceedances should future listings of non-vascular aquatic plants warrant

additional evaluation of Federal actions.

b Terrestrial-phase amphibians and reptiles estimated using birds as surrogates. Aquatic-phase amphibians

estimated using freshwater fish as surrogates.

C Animals that feed on aquatic organisms may be indirectly affected.

d Indirect effects may be caused by plants that rely on fungus for growth or reproduction or that rely on

mammals as pollinators or seed dispersers.

LOCATES is used to identify listed species that may be located within the same county

as the proposed new uses. The LOCATES runs are listed in Appendix H.

20

--,....-'----------------
Appendix A: Registered Uses of Pyraclostrobin

21
Pyraclostrobin is presently registered for the following uses:

• BAS 500ST ® Seed Treatment Fungicide (Registration No. 7969-240 issued

May 25,2007)

-Barley (ground and aerial applications)

-Brassica vegetables (ground and aerial applications)

-Bulb vegetables (ground and aerial applications)

-Com (field, pop, sweet, and seed) (ground and aerial applications)

-Curcurbits (ground and aerial applications)

-Legume veges (ground and aerial applications)

-Peanuts (ground and aerial applications)

-Rye (ground and aerial applications)

-Soybean (ground and aerial applications)

-Sugar-beets (ground and aerial applications)

-Sunflower (ground and aerial applications)

-Wheat (ground and aerial applications)

• BAS 516ST ® Seed Treatment Fungicide (Registration No. 7969-248 issued

May 30, 2007)

-Brassica vegetables (ground and aerial applications)

-Bulb vegetables (ground and aerial applications)

-Curcurbit vegetables (ground and aerial applications)

-Legume vegetables (ground and aerial applications)

-Soybean (ground and aerial applications)

-Sunflower (ground and aerial applications)

• Cabrio EG ® Fungicide (Registration No. 7969-187 issued June 5, 2004)

-Berries (ground and aerial applications)

-Brassica head and stem crops (ground and aerial applications)

-Brassica crops (ground and aerial applications)

-Bulb vegestables (ground and aerial applications)

-Curcurbit vegetables (ground and aerial applications)

-Fruiting vegetables (ground and aerial applications)

-Grapes (ground and aerial applications)

-Hops (ground and aerial applications)

-Leafy vegetables (ground and aerial applications)

-Leaves ofroots and tubors (ground and aerial applications)

22
Cabrio EG ® Fungicide (continued) (Registration No. 7969-187 issued June 5,

2004)

-Pistaccio (ground and aerial applications)

-Pomefruit (ground and aerial applications)

-Root vegetables (ground and aerial applications)

-Stone fruits (ground and aerial applications)

-Strawberries (ground and aerial applications)

-Tree nuts (ground and aerial applications)

• Cabrio Team ® Fungicide (Registration No. 7969-229 issued July 5, 2005)

-Bulb vegetables (ground and aerial applications)

-Curcurbit vegetables (ground and aerial applications)

-Fruiting vegetables (ground and aerial applications)

• Headline ® Fungicide (Registration No. 7969-186 issued April 4, 2007)

-Barley (ground and aerial applications)

-Citrus crops (ground and aerial applications)

-Corn (ground and aerial applications)

-Dried-shelled peas and beans (ground and aerial applications)

-Edible podded legume vegetables (ground and aerial applications)

-Seed grass (ground and aerial applications)

-Mint (ground and aerial applications)

-Peanut (ground and aerial applications)

-Pecan (ground and aerial applications)

-Rye (ground and aerial applications)

-Soybean (ground and aerial applications)

• Headline ® Fungicide (Registration No. 7969-186 issued November 24,2009)

-Alfalfa (ground and aerial applications)

• Headline ® Fungicide (Registration No. 7969-186 issued March 6, 2009)

-Sorghum (ground and aerial applications)

• Headline ® Fungicide (Registration No. 7969-186 issued April 17, 2009)

-dry beans, peanuts, and sunflower crops (in-furrow)

• Honor ® Fungicide (Registration No. 7969-248 issued May 30, 2007)

-Golf course turfgrass (ground applications only)

• Insignia ® Fungicide (Registration No. 7969-184 issued May 15,2007)

-GolfCourse Turfgrass (ground applications only)

-Ornamentals (ground applications only)

• Pristine ® Fungicide (Registration No. 7969-199 issued April 26, 2006)

-Berries (ground and aerial applications)

-Bulb vegetables (ground and aerial applications)

23
-Curcurbit vegetables (ground and aerial applications)

-Soybeans (ground and aerial applications)

-Spinach (ground and aerial applications)

-Stone fruits (ground and aerial applications)

-Strawberries (ground and aerial applications)

-Tree nuts (ground and aerial applications)

-Almonds (ground and aerial applications)

• Stamina ® Seed Treatment Fungicide (Registration No. 7969-266 issued

January 23, 2008)

-Barley

-Brassica vegetables

-Bulb vegetables

-Com (field, pop, sweet, com for seed, production

-Cucurbits

- Legume vegetables (dried shelled peas and beans)

-Peanut

-Rye

-Soybean

- Sugar beet

-Sunflower

-Wheat

• Stamina ® Seed Treatment Fungicide (Registration No. 7969-266 issued March

6,2009)

-Sorghum

• Stamina ® Seed Treatment Fungicide (Registration No. 7969-266 issued

November, 24, 2009)

-Alfalfa

24
Appendix B: Input Parameters Used in PRZMIEXAMS Modeling

25
PRZMlEXAMS Input Parameters for Proposed Pyraclostrobin Uses I

Parameter Input Value and Unit Comment Source

Chemical Application Method 2 Foliar Application !GuidanCe

- (CAM) 4 Seed treatment

oem. canola' I Planting Depth 3.81 ern. sweet com ,

0.635 em, sugar beet

IMRID 45118627 Hydrolysis I (t1/2) odays stable

Spray drift and application Drfit- Efficiency -

Aerial: 0.05 Aerial: 0.95 iGuidance efficiency Seed Treatment: 0.0 Seed Treatment: 1.00

1248.14 days Upper 90th percentile of MRlD 45118631

Aerobic soil metabolism (tI/2) I

the mean of half-lives in MRlD 45118632

I six studies MRlD 45367504

496.28 days No acceptable study No Acceptable ,

Isubmitted and the studies. EFED

Aerobic aquatic metabolism (tI/2) pesticide shows IGuidance,

.insignificant hydrolysis; ,

2 times the aerobic soil

half-life input i

[Anaerobic aquatic metabolism 6.6 days 2 times the half-life (3.3 No Studies

days) ofthe anaerobic submitted ' (t1/2) [soil metabolism.

0.08 days IMaximum dark IEFED Guidan,,; I

Aquatic photolysis (tI/2) [controlled aqueous MRlD 45118629 ,

photolysis half-life of

I two studies

Vapor pressure 1.95E-8 Product Chemistry

Henry's Law constant (20°C) 4.13 x lO-Yatm'm'/mol Product Chemistry

Solubility in water (pH 7, 20°C) 2.41 Product Chemistry

Molecular weight 388 glmole IProduct Chemistry

7,889 (Germany sand) I

.160,000 (Germany loamy sand) i

7,889 (Germany sandy loam)

19,304

6,000 (USA loamy sand) 45160502,45160503,

]Koc 9,000 (USA sandy loam) 45118703,45118701,

9,436 (Canada sandy loam) 45118702

I 9,304 (mean Koc of six soil

I I Istudies) I ,

1 Typical agricultural practice for rapeseed is to apply the seeds to the surface ofthe soil, followed by a

process of "chaining in" the seeds (e.g., pulling chains behind a vehicle to incorporate the seeds)

(OMAFRA staff, 2009). Due to the inconsistency ofthis method, a conservative incorporation depth of 0

(zero) inches is used.

26
Appendix C: Example PRZMlEXAMS Output Files

27
MS Com - Aerial Application

stored as MSCRnA.out

Chemical: Pyraclostrobin

PRZM environment: MScomSTD.txt modified Tueday, 29 May 2007 at 12:57:40

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:08

Metfile: w03940.dvf modified Tueday, 26 August 2008 at 05: 14:14

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.461 0.3241 0.1295 0.094090.06759 0.02221

1962 0.275 0.1878 0.06892 0.02616 0.0232 0.01223

1963 0.5203 0.3618 0.1371 0.090890.06191 0.02173

1964 0.3804 0.2577 0.09207 0.05016 0.0468 0.02967

1965 0.5296 0.3708 0.1499 0.0635 0.044160.02088

1966 0.3456 0.2472 0.1096 0.05048 0.0465 0.02075

1967 0.6776 0.4654 0.1667 0.0856 0.0621 0.02262

1968 0.2787 0.1892 0.06563 0.028 0.0244 0.01377

1969 0.2741 0.2022 0.07112 0.03667 0.03606 0.01572

1970 0.3547 0.2458 0.08675 0.05765 0.04882 0.02336

1971 0.2758 0.1876 0.09351 0.05431 0.04567 0.0247

1972 0.2742 0.1867 0.072740.029120.023140.01543

1973 0.2753 0.1882 0.065860.03045 0.02427 0.01693

1974 0.5928 0.4104 0.1808 0.06842 0.05207 0.02722

1975 0.3598 0.2482 0.1504 0.09606 0.08287 0.03063

1976 0.4185 0.2864 0.1017 0.06588 0.04905 0.02125

1977 0.3003 0.2058 0.106 0.0608 0.046190.02318

1978 0.278 0.1879 0.06465 0.03577 0.0295 0.01792

1979 0.841 0.5793 0.2386 0.1529 0.1126 0.04939

1980 0.2758 0.1863 0.0639 0.0351 0.03287 0.01788

1981 0.6535 0.4504 0.1618 0.1082 0.07552 0.02489

1982 0.6518 0.4532 0.1782 0.1154 0.09221 0.03805

1983 0.2799 0.1927 0.071760.0374 0.03255 0.02444

1984 0.275 0.1856 0.064970.05151 0.047640.02241

1985 0.4033 0.2779 0.1023 0.05168 0.05134 0.02052

1986 0.3486 0.2543 0.1219 0.0682 0.05001 0.02127

1987 0.4838 0.3866 0.1573 0.08013 0.05876 0.02114

1988 0.274 0.1853 0.06377 0.03936 0.03375 0.01901

1989 0.7166 0.5061 0.2748 0.119 0.08328 0.02874

1990 0.2795 0.1888 0.06469 0.03362 0.02455 0.01787

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.841 0.5793 0.2748 0.1529 0.1126 0.04939

0.0645161290322581 0.7166 0.5061 0.2386 0.119 0.09221 0.03805

0.0967741935483871 0.6776 0.4654 0.1808 0.1154 0.08328 0.03063

0.129032258064516 0.6535 0.4532 0.1782 0.1082 0.08287 0.02967

0.161290322580645 0.6518 0.4504 0.1667 0.09606 0.07552 0.02874

0.193548387096774 0.5928 0.4104 0.1618 0.09409 0.06759 0.02722

0.225806451612903 0.5296 0.3866 0.1573 0.090890.0621 0.02489

0.258064516129032 0.5203 0.3708 0.1504 0.0856 0.06191 0.0247

0.290322580645161 0.4838 0.3618 0.1499 0.08013 0.05876 0.02444

0.32258064516129 0.461 0.3241 0.1371 0.06842 0.05207 0.02336

0.354838709677419 0.4185 0.2864 0.1295 0.0682 0.051340.02318

28
0.387096774193548 0.4033 0.2779 0.1219 0.06588 0.05001 0.02262

0.419354838709677 0.3804 0.2577 0.1096 0.0635 0.04905 0.02241

0.451612903225806 0.3598 0.2543 0.106 0.0608 0.04882 0.02221

0.483870967741936 0.3547 0.2482 0.1023 0.05765 0.04764 0.02173

0.516129032258065 0.3486 0.2472 0.1017 0.05431 0.0468 0.02127

0.548387096774194 0.3456 0.2458 0.09351 0.05168 0.0465 0.02125

0.580645161290323 0.3003 0.2058 0.092070.05151 0.046190.02114

0.612903225806452 0.2799 0.2022 0.08675 0.05048 0.04567 0.02088

0.645161290322581 0.2795 0.1927 0.072740.050160.044160.02075

0.67741935483871 0.2787 0.1892 0.07176 0.03936 0.03606 0.02052

0.709677419354839 0.278 0.1888 0.07112 0.0374 0.03375 0.01901

0.741935483870968 0.2758 0.1882 0.06892 0.03667 0.03287 0.01792

0.774193548387097 0.2758 0.1879 0.06586 0.03577 0.03255 0.01788

0.806451612903226 ·0.2753 0.1878 0.06563 0.0351 0.0295 0.01787

0.838709677419355 0.275 0.1876 0.064970.033620.02455 0.01693

0.870967741935484 0.275 0.1867 0.06469 0.03045 0.0244 0.01572

0.903225806451613 0.2742 0.1863 0.06465 0.02912 0.02427 0.01543

0.935483870967742 0.2741 0.1856 0.0639 0.028 0.0232 0.01377

0.967741935483871 0.274 0.1853 0.06377 0.02616 0.02314 0.01223

0.1 0.67519 0.46418 0.18054 0.11468 0.083239 0.030534

Average of yearly averages: 0.0228603333333333

Inputs generated by pe5.pl- Novemeber 2006

Data used for this run:

Output File: MSCRnA

Metfile: w03940.dvf

PRZM scenario: MScomSTD.txt

EXAMS environment file: pond298.exv

Chemical Name: Pyrac1ostrobin

Description Variable Name Value Units Comments

Molecular weightmwt 388 glmol

Henry's Law Const. henry 4.13E-9 atm-m-S/mol

Vapor Pressure vapr 1.95E-8 torr

Solubility sol 2.41 mglL

Kd Kd mglL

Koc Koc 9304 mglL

Photolysis half-life kdp .08 days Half-life

Aerobic Aquatic Metabolism kbacw 496.28 days Halfife

Anaerobic Aquatic Metabolism kbacs 6.6 days Halfife

Aerobic Soil Metabolism asm 248.14 days Halfife

Hydrolysis: pH 7 0 days Half-life

Method: CAM 2 integer See PRZM manual

Incorporation Depth: DEPI 0 em

Application Rate: TAPP 0.112 kg/ha

Application Efficiency: APPEFFO.95 fraction

Spray Drift DRFT 0.05 fraction of application rate applied to pond

Application Date Date 01-06 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17: FILTRA

IPSCNDI

UPTKF

Record 18: PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

29
Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

MN Sugar Beet - Seed Treatment

stored as MNSugST.out

Chemical: Pyraclostrobin

PRZM environment: MNsugarbeetSTD.txt modified Tueday, 29 May 2007 at 12:57:22

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05: 14:08

Metfile: w14914.dvf modified Tueday, 26 August 2008 at 05:15:16

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.3301 0.2355 0.1059 0.044690.031640.009657

1962 0.5678 0.414 0.189 0.099540.070950.01872

1963 0.1647 0.1176 0.053590.0209 0.022560.006412

1964 0.4593 0.3258 0.1265 0.047940.03871 0.01076

1965 0.078590.05604 0.02454 0.01571 0.0127 0.005361

1966 0.08883 0.06301 0.03404 0.0179 0.01245 0.004593

1967 0.02581 0.018590.009525 0.007001 0.005946

1968 0.023040.016790.009207 0.007606 0.006769

1969 0.2358 0.166 0.091130.040830.03241 0.01008

1970 0.1799 0.1266 0.04907 0.02098 0.01781 0.008465

1971 0.2464 0.1749 0.073170.0313 0.021240.01137

1972 0.1781 0.1263 0.0506 0.020390.016170.005642

1973 0.1113 0.07925 0.03403 0.025040.018370.005924

1974 0.2671 0.1893 0.081930.053550.037160.01428

1975 0.3913 0.3172 0.1856 0.077440.053680.01461

1976 0.007729 0.005828 0.002817 0.002079

0.0004062

1977 0.635 0.4451 0.1706 0.0862 0.076 0.02276

1978 0.3642 0.2575 0.1002 0.043960.03803 0.01081

1979 0.1034 0.0731 0.029370.020970.014660.007322

1980 0.1589 0.1126 0.050670.031760.022570.00664

1981 0.4889 0.3506 0.1666 0.072 0.049840.01278

1982 0.2595 0.1847 0.078580.03677 0.02686 0.008263

1983 0.1102 0.077190.041630.02 0.015560.005328

1984 0.7212 0.5146 0.2206 0.08655 0.057990.01942

1985 0.2583 0.1863 0.1031 0.058420.04577 0.01233

1986 0.2267 0.16 0.06396 0.0322 0.02568 0.008676

1987 0.1574 0.112 0.061390.024420.021750.005959

1988 0.0733 0.055290.021780.0135 0.009827 0.003167

1989 0.1784 0.1361 0.061110.030170.021160.008086

1990 0.2641 0.1882 0.093680.047790.032760.009079

0.001623

0.00271

0.001489

Sorted results

Prob. Peak 96 hr

0.032258064516129

0.0645161290322581

0.0967741935483871

0.129032258064516

0.161290322580645

0.193548387096774

0.225806451612903

0.258064516129032

0.290322580645161

0.32258064516129

21 Day

0.7212

0.635

0.5678

0.4889

0.4593

0.3913

0.3642

0.3301

0.2671

0.2641

60 Day

0.5146

0.4451

0.414

0.3506

0.3258

0.3172

0.2575

0.2355

0.1893

0.1882

90 Day Yearly

0.2206 0.09954 0.076 0.02276

0.189 0.08655 0.07095 0.01942

0.1856 0.0862 0.057990.01872

0.1706 0.077440.05368 0.01461

0.1666 0.072 0.049840.01428

0.1265 0.05842 0.04577 0.01278

0.1059 0.053550.03871 0.01233

0.1031 0.047940.03803 O.oI137

0.1002 0.047790.037160.01081

0.09368 0.04469 0.03276 0.0 1076

30
0.354838709677419 0.2595 0.1863 0.09113 0.043960.03241 0.01008

0.387096774193548 0.2583 0.1847 0.08193 0.04083 0.031640.009657

0.419354838709677 0.2464 0.1749 0.07858 0.03677 0.02686 0.009079

0.451612903225806 0.2358 0.166 0.07317 0.0322 0.02568 0.008676

0.483870967741936 0.2267 0.16 0.06396 0.03176 0.02257 0.008465

0.516129032258065 0.1799 0.1361 0.06139 0.0313 0.02256 0.008263

0.548387096774194 0.1784 0.1266 0.06111 0.030170.021750.008086

0.580645161290323 0.1781 0.1263 0.053590.02504 0.02124 0.007322

0.612903225806452 0.1647 0.1176 0.050670.024420.02116 0,00664

0.645161290322581 0.1589 0.1126 0.0506 0.020980.018370.006412

0.67741935483871 0.1574 0.112 0.049070.020970.01781 0.005959

0.709677419354839 0.1113 0.07925 0.04163 0.0209 0.016170.005924

0.741935483870968 0.1102 0.077190.034040.020390.015560.005642

0.774193548387097 0.1034 0.0731 0.03403 0.02 0.01466 0.005361

0.806451612903226 0.088830.063010.029370.0179 0.0127 0.005328

0.838709677419355 0.078590.056040.024540.01571 0.01245 0.004593

0.870967741935484 0.0733 0.055290.021780.0135 0.009827 0.003167

0.903225806451613 0.02581 0.018590.009525 0.007606 0.006769 0.00271

0.935483870967742 0.023040.016790.009207 0.007001 0.005946

0.001623

0.967741935483871 0.007729 0.005828 0.002817 0.002079

0.001489 0.0004062

0.1 0.55991 0.407660.1841 0.085324 0.057559 0.018309

Average of yearly averages: 0.00904110666666667

Inputs generated by pe5.pl- Novemeber 2006

Data used for this run:

Output File: MNSugST

Metfile: w14914.dvf

PRZM scenario: MNsugarbeetSTD.txt

EXAMS environment file: pond298.exv

Chemical Name: Pyraclostrobin

Description Variable Name Value Units Comments

Molecular weightmwt 388 g/mol

Henry's Law Const. henry 4.13E-9 atm-m/S/rnol

Vapor Pressure vapr 1.95E-8 torr

Solubility sol 2.41 mg/L

Kd Kd mg/L

Koc Koc 9304 mg/L

Photolysis half-life kdp .08 days Half-life

Aerobic Aquatic Metabolism kbacw 496.28 days Halfife

Anaerobic Aquatic Metabolism kbacs 6.6 days Halfife

Aerobic Soil Metabolism asm 248.14 days Halfife

Hydrolysis: pH 7 0 days Half-life

Method: CAM 4 integer See PRZM manual

Incorporation Depth: DEPI 0.635 em

Application Rate: TAPP 0.118 kg/ha

Application Efficiency: APPEFF 1.0 fraction

Spray Drift DRFT 0.0 fraction of application rate applied to pond

Application Date Date 01-05 dd/mm or dd/mmm or dd-rnm or dd-mmm

Record 17: FILTRA

IPSCND1

UPTKF

Record 18: PLVKRT

31
PLDKRT

FEXTRC

Flag for Index Res. Run

Flag for runoff calc.

o

IR EPA Pond

RUNOFF none none, monthly or total(average of entire run)

32
Appendix D: Risk Quotient Calculations

33
The Risk Quotient Method

The Risk Quotient method is the approach used by EFED in estimating the risk to aquatic and terrestrial

species. This method involves integrating the results of exposure and ecotoxicity data. For this method,

risk quotients (RQs) are calculated by dividing the exposure estimates by the acute or chronic ecotoxicity

values.

RQ = EXPOSURE/TOXICITY

RQs are then compared to OPP's levels ofconcem (LOCs). These LOCs are criteria used by OPP to

indicate potential risk to non-target organisms and the need to consider regulatory action. EFED has

defined LOCs for acute risk, potential restricted use classification and for endangered species. A

description ofthe method and LOCs for non-target organisms is presented in the following Table.

34
Table D-I-Risk Quotient Values and Meanings

RiskPresumptioos aDdLOCs

Risk Presumption RQ LOC

Birds'

Acute Risk EECILC,o or LO,oIsqft or LO,oIday 0.5

Acute Restricted Use EECILC,o or LO,oIsqft or LO'o/day (or LO,o < 50 rng/kg) 0.2

Acute Endangered Species EECILC,o or LO,oIsClft or LO'o/day 0.1

Chronic Risk EEC/NOAEC 1

Wild Mammals'

Acute Risk EECILC,o or LO,oIsqft or LO,oIday 0.5

Acute Restricted Use EECILC,o or LO,oIsClft or LO,oIday (or LO,o < 50 rug/kg) 0.2

Acute Endangered Species EECILC,o or LO,oIsQftor LO,oIday 0.1

Chronic Risk EEC/NOAEC I

Aquatic Animals'

Acute Risk EECILC,o or EC,o 0.5

Acute Restricted Use EECILC,o or EC,o 0.1

Acute Endangered Species EECILC,o or EC,o 0.05

Chronic Risk EEC/NOAEC I

Terrestrial and Semi-Aquatic Plants

Acute Risk EEC/EC2, I

Acute Endangered Species EEC/ECo, or NOAEC I

Aquatic Plants'

Acute Risk EEC/EC,o 1

Acute Endangered Species EEC/ECo, or NOAEC I

1 LO,oIsqft = (mg/sqft) I (LO,o * wt. of animal)

LO,oIday = (mg oftoxicant consumed/day) I (LO,o * wt. of animal)

2 EEC = (ppm or ppb) in wa

35
Table D2- Coronet (seed treatment to Canola and Crambe 0.012 Ibs ai/acre) and aquatic risk quotients

Aquatic Acute Chronic Peak 21-day 60-Day Acute Chronic LOC

Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence

Value (ug/L) (ug/L) (ug/l.) (ug/L)

(1lg!L)

Bluegill LC,o 96 N/A 0.38 0.11 0.06 0.03 N/A None

Sunfish hr: I\.4

(Lepomis

macrochirusColdwater

fish)

Rainbow Trout LC,o 96 N/A 0.38 0.11 0.06 0.061 Yes Listed

(Oncorhynchus hr: 6.2 Species OC

mykiss-Warm- exceeded

water fish)

Fathead N/A NOAEC=4.14 0.38 0.11 0.06 0.01 None

Minnow

(Pimephales

promelasi

Rainbow Trout N/A NOAEC-2.35 0.38 0.11 0.06 0.03 None

(Oncorhynchus

mykiss)

Sheepshead LC,o 96 hr NOAEC=10.8 0.38 0.11 0.06 <0.01 <0.01 None

Minnow : 76.9

(Cyprinodon

variegatesSalt-Water

Fish)

Daphnia EC,o 48 hr 0.38 0.11 0.06 0.024 NO

magna : 15.7 a.i.

(14.2-17.4)

~g/L

Daphnia NOAEC : 4 ug 0.38 0.11 0.06 0028 None

magna a.i. /L

EC,o 96 hr 0.38 0.11 0.06 0.034 None

Eastern Oyster : 12.5

(Crassostrea a.i.(9.2- virginica) 17.1) Mollusk Shell ~g/L Deposition

LC,o 96 hr 0.38 0.11 0.06 0.09 Yes Acute

Mysid Shrimp : 4.16 Listed (Americamysis Species Use bahia) LOCs are

exceeded

Mysid Shrimp NOAEC=\.06! 0.38 0.11 0.06 0.06 None

(Americamysis

bahia)

36
Table D 3- Coronet (seed treatment to Canola and Crambe O.0121bs at/acre) and aquatic plant risk quotients

Aquatic Plants Toxicity Value (p.g/L) Peak EEC Value Risk Quotient Value Aquatic Plant LOC

(J12!L) Exceedence?

0.38 <0.01(when using the None Freshwater Green Algae ECso 96 hr: 152 (143- ECso Value) and 0.03 (Pseudokirchneriella 162) ug a.i. /L (when using the subcapitata) (biomass) NOAEC value) NOAEC: 14.0 ug

a.i./L

(Biomass and Number

of cells/ml)

0.38 <0.01 None

Freshwater Blue-Green ECso 120 hr : > 1780

Algae ug a.i./L

(Anabaena fios-aquae) (No. of cells/ml and

growth rate)

NOAEC : 1780 ug

a.i./L

(Growth rate and

Number of cells/ml)

0.38 0.32 None

Freshwater diatoms ECso 120 hr : 1.50

(Navicula pelliculosa) «1.18 - 3.83) ug a.i./L

(No. of cells/ml)

NOAEC: 1.18 ug

a.i./L

(Number of cells/ml

and Growth rate)

0.38 <0.01 (when using the None Marine diatoms ECso 120 hr: 64.7 (50 ECso value) and 0.038 (Skeletonema costatum) - 84) ug a.i./L (No. of (when using the cells/ml) NOAEC value)

NOAEC: 1O.01lg

a.i./L

(Number of cells/ml)

Duckweed sc., 14 days : 1720 0.38 <0.01 when using either None

(Lemna gibba) ug a.i./L the ECso or NOAEC

(Plant biomass) Values

NOAEC : 896 ug a.i./L

(Number of normal

fronds and plant

biomass)

37
Table D4-Aquatic Organism Risk Quotients of BAS 500 20 F (I application at O.llbs ai/acre applied to corn (field, pop, and

seed production

Aquatic Acute Chronic Peak 21-day 60-Day Acute Chronic LOC

Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence

Value (Ilg/L) (ug/L) (ug/L) (ug/L)

(ug/L)

Bluegill LC5096 N/A 0.68 0.18 0.11 0.06 N/A Acute Listed

Sunfish hr: 11.4 Species

(Lepomis LOC

macrochirus- Exceeded

Coldwater

fish)

Rainbow Trout LC,o 96 N/A 0.68 018 0.11 0.11 Yes Acute

(Oncorhynchus hr: 6.2 Restricted

mykiss-Warm- Use and

water fish) Acute

Listed

Species OC

exceeded

Fathead N/A NOAEC-4.14 0.68 0.18 0.11 0.03 None

Minnow

(Pimepha/es

promelasy

Rainbow Trout N/A NOAEC-2.35 0.68 018 0.11 0.05 None

(Oncorhynchus

mykiss)

Sheepshead LC,o 96 hr NOAEC=10.8 0.68 0.18 0.11 <0.01 0.01 None

Minnow : 76.9

(Cyprinodon

variegatesSalt-Water

Fish)

Daphnia EC,o 48 hr 0.68 0.18 0.11 0.043 None

magna : 15.7 a.i.

(14.2-17.4)

Ilg/L

Daphnia NOAEC: 4 ug 0.68 0.18 0.11 0.05 None

magna a.i. /L

EC,o 96 hr 0.68 0.18 0.11 0.054 Acute Listed

Eastern Oyster : 12.5 Species (Crassostrea a.i.(9.2- LOC

virginica) 17.1) exceeded

Mollusk Shell J.lg/L Deposition

LC,o 96 hr 0.68 018 0.11 0.20 Acute

Mysid Shrimp : 4.16 Restricted

(Americamysis Use and

bahia) Acute Listed

Species

LOCs are

exceeded

Mysid Shrimp NOAEC=1.06

1 0.68 0.18 0.11 0.20 None

(Americamysis

bahia)

38
Table D5-Aquatic Plants Risk Quotients for BAS 500 20 F (I application at O.llbs ai/acre applied to corn (field, pop, and seed

production)

Aquatic Plants Toxicity Value (J1gIL) Peak EEC Value Risk Quotient Value Aquatic Plant LOC

(112!L) Exceedence?

0.68 <0.01(when using the None Freshwater Green Algae EC,o 96 hr : 152 (143- EC,o Value) and 0.05 (Pseudokirchneriella 162) ug a.i. /L (when using the subcapitata) (biomass) NOAEC value) NOAEC: 14.0 ug

a.i.lL

(Biomass and Number

of cells/ml)

0.68 <0.01 None

Freshwater Blue-Green EC,o 120 hr: > 1780

Algae ug a.i./L

(Anabaenajlos-aquae) (No. of cells/ml and

growth rate)

NOAEC : 1780 ug

a.i./L

(Growth rate and

Number of cells/ml)

0.68 0.5 (when using the None Freshwater diatoms EC,o 120 hr : 1.50 EC,o Value) and 0.60 (Navicula pelliculosa) «1.18 - 3.83) ug a.i./L (when using the (No. of cells/ml) NOAEC value) NOAEC: 1.18 ug

a.i.lL

(Number of cells/ml

and Growth rate)

0.68 0.01 (when using the None Marine diatoms EC,o 120 hr: 64.7 (50 EC,o value) and 0.068 (Skeletonema costatum) - 84) ug a.i./L (No. of (when using the cells/ml) NOAEC value)

NOAEC: 10.01lg

a.i.lL

(Number of cells/ml)

Duckweed EC,o 14 days : 1720 0.68 <0.01 when using either None

(Lemna gibba) ug a.i./L the EC,o or NOAEC

(Plant biomass) Values

NOAEC : 896 ug a.i./L

(Number of normal

fronds and plant

biomass)

39
Table D6-Aquatic Orzamsm Risk Quotients of STAMINA to sweet corn seeds (O.003Ibs. a.i.lacre)

Aquatic Acute Chronic Peak 2I-day 60-Day Acute Chronic LOC

Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence

Value (~g/L) (ug/l.) (ug/L) (ug/L)

[ug/L)

Bluegill LC,o 96 N/A 0.02 0.007 0.003 <0.01 N/A None

Sunfish hr: 11.4

(Lepomis

macrochirusColdwater

fish)

Rainbow Trout LCso96 N/A 0.02 0.007 0.003 <0.01 None

(Oncorhynchus hr: 6.2

mykiss-Warrnwater fish)

Fathead N/A NOAEC=4.14 0.02 0.007 0.003 <0.01 None

Minnow

(Pimephales

promelasi

Rainbow Trout N/A NOAEC-2.35 0.02 0.007 0.003 <0.01 None

(Oncorhynchus

mykiss)

Sheepshead LC,o 96 hr NOAEC=10.8 0.02 0.007 0.003 <0.01 <0.01 None

Minnow : 76.9

(Cyprinodon

variegatesSalt-Water

Fish)

Daphnia ECso 48 hr 0.02 0.007 0.003 <0.01 None

magna : 15.7 a.i.

(14.2-17.4)

~g/L

Daphnia NOAEC : 4 ug 0.02 0.007 0.003 <0.01 None

magna a.i. /L

ECso96 hr 0.02 0.007 0.003 <0.01

Eastern Oyster : 12.5

(Crassostrea a.i.(9.2- virginica) 17.1) Mollusk Shell ~g/L Deposition

LC,o 96 hr 0.02 0.007 0.003 <0.01

Mysid Shrimp : 4.16

(Americamysis

bahia)

Mysid Shrimp NOAEC=106

1 0.02 0.007 0.003 <0.01 None

(Americamysis

bahia)

40
Table D7-Aquatic Plants Risk Quotients of STAMINA to sweet corn seeds (O.003Ibs. a.iJacre) using Peak EEC values

Aquatic Plants Toxicity Value (1tWL) Peak EEC Value Risk Quotient Value Aquatic Plant LOC

(I1lUL) Exceedence?

0.02 <O.OI(when using the None Freshwater Green Algae EC,o 96 hr: 152 (143- ECsoor NOAEC

(Pseudokirchneriella 162) ug a.i. /L values) subcapitatai (biomass)

NOAEC: 14.0 ug

a.i./L

(Biomass and Number

of cells/ml)

0.02 <0.01 None

Freshwater Blue-Green ECso 120 hr: > 1780

Algae ug a.i./L

(Anabaena flos-aquaei (No. of cells/ml and

growth rate)

NOAEC : 1780 ug

a.i./L

(Growth rate and

Number of cells/ml)

0.02 0.013 (when using the None Freshwater diatoms ECso 120 hr : 1.50 ECsoValue) and 0.017 (Navicula pelliculosa) «1.18 - 3.83) ug a.i./L (when using the (No. of cells/ml) NOAEC value) NOAEC: 1.18 ug

a.i./L

(Number of cells/ml

and Growth rate)

0.02 <0.01 (when using the None Marine diatoms ECso 120 hr: 64.7 (50 ECso value) and 0.002 (Skeletonema costatumi - 84) ug a.i./L (No. of (when using the cells/ml) NOAEC value)

NOAEC : 10.0 ug

a.i./L

(Number of cells/ml)

Duckweed ECso 14 days: 1720 0.02 <0.01 when using either None

(Lemna gibba) ug a.i./L the ECsoor NOAEC

(Plant biomass) Values

NOAEC : 896 ug a.i.lL

(Number of normal

fronds and plant

biomass)

41
Table D8-AQuatic Organism Risk Quotients of STAMINA to sugar beets seeds (0.104Ibs. a.i.zacre)

Aquatic Acute Chronic Peak 21-day 60-Day Acute Chronic LOC

Organisms Toxicity Toxicity Value EEC EEC EEC RQ RQ Exceedence

Value (flglL) (ug/L) (ug/L) (flg/L)

(ug/l.)

Bluegill LCso 96 N/A 0.56 018 0.06 0.05 N/A Acute Listed

Sunfish hr: 11.4 Species

(Lepomis LOC is

macrochirus- triggered

Coldwater

fish)

Rainbow Trout LCso 96 N/A 0.56 0.18 0.06 0.10 Acute

(Oncorhynchus hr: 6.2 Restricted

mykiss-Warm- Use LOC is

water fish) triggered

and Acute

Listed

Species Use

is exceeded

Fathead N/A NOAEC-4.14 0.56 0.18 0.06 0.01 None

Minnow

(Pimephales

promelasi

Rainbow Trout N/A NOAEC=2.35 0.56 0.18 0.06 0.03 None

(Oncorhynchus

mykiss)

Sheep shead LCso 96 hr NOAEC-IO.8 0.56 0.18 0.06 <0.01 <0.01 None

Minnow : 76.9

(Cyprinodon

variegatesSalt-Water

Fish)

Daphnia ECso 48 hr 0.56 018 0.06 0.04 None

magna : 15.7 a.i.

(14.2-174)

J.lg/L

Daphnia NOAEC : 4 ug 0.56 0.18 0.06 0.05 None

magna a.i. /L

ECso 96 hr 0.56 0.18 0.06 0.04 None

Eastern Oyster : 12.5

(Crassostrea a.i.(9.2- virginicai 17.1) Mollusk Shell flglL Deposition

LCso 96 hr 0.56 0.18 0.06 0.13 Acute

Mysid Shrimp : 4.16 Restricted

iAmericamysis Use and

bahia) Acute Listed

Species

LOCs are

exceeded

Mysid Shrimp NOAEC=1.06

1 0.56 0.18 0.06 017 None

(Americamysis

bahia)

42
Table D9-AQuatic Plants Risk Quotients of STAMINA to suaar beet seeds 0.104 lbs, a.i.lacre) uslna Peak EEC values

Aquatic Plants Toxicity Value (!Jg/L) Peak EEC Value Risk Quotient Value Aquatic Plant LOC

(JlI!:/L) Exceedence?

0.56 <0.01(when using the None Freshwater Green Algae ECso 96 hr: 152 (143- ECso) and 0.04 (whe (Pseudokirchneriella 162) ug a.i. /L using the NOAEC subcapitata) (biomass) values) NOAEC: 14.0 ug

a.i./L

(Biomass andNumber

ofcells/ml)

0.56 <0.01 None

Freshwater Blue-Green ECso 120 hr : > 1780

Algae ug a.i./L

(Anabaena flos-aquaej (No. of cells/ml and

growth rate)

NOAEC : 1780 ug

a.i./L

(Growth rate and

Number of cells/ml)

0.56 0.40 (when using the None Freshwater diatoms ECso 120 hr : 1.50 ECsoValue) and 0.50 (Navicula pelliculosa) «1.18 - 3.83) ug a.i./L (when using the (No. of cells/ml) NOAEC value) NOAEC: 1.18 ug

a.i./L

(Number of cells/ml

and Growth rate)

0.56 <0.01 (when using the None

Marine diatoms ECso 120 hr : 64.7 (50 ECso value) and 0.056 (Skeletonema costatum) - 84) Ilg a.i./L (No. of (when using the cells/ml) NOAEC value)

NOAEC: 10.0 ug

a.i./L

(Number of cells/ml)

Duckweed ECso 14 days: 1720 0.56 <0.01 when using either None

(Lemna gibba) ug a.i.zl, the ECso or NOAEC

(Plant biomass) Values

NOAEC : 896 ug a.i./L

(Number of normal

fronds and plant

biomass)

43
Appendix E: T-REX Modeling

44
Acute

0.00

0.00

0.00

0.00

Note: To provide risk management with the maximum possible information,

it is recommended that both the dose-based and concentration-based

RQs be calculated when data are available

45
Pyraclostrobin

Mammalian Results

Corn

Acute

#DIVIOI

#DIVIOI

#DIV/OI

#DIV/OI

Chronic

0.04

0.02

0.02

0.00

46

Upper bound Kenasa Residues
47

DoItIIlnputs .... in blua

.0200

OOסס0.

OOסס0.

OOסס0.

OOסס0.

0.1000

0.0000

0.0100

OOסס0.

0.0000

0.0000

OOסס0.

OOסס0.

0.0000

0.0000

0.0000

0.0000

0.0000

0.0000

0.0000

0.0000

0.0000

1.3300

0.0000

0.0000
0.00 200.00 50.82 42.37 0.03

-- 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.01 1000.00 253.09 211.87 0.08

0.00 0.00 0.00 0.00 0.00

0.00 100.00 25.31 21.19 0.02

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

0.11 13300.00 3366.08 2817.90 1.11

0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00

Acutll .1 Acutll • 2 Chronic Acutll,l Acutll Chronic

0.05 0.00 0.19 0.00 0.00 0.34

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.24 0.00 0.94 0.02 0.00 1.72

0.00 0.00 0.00 0.00 0.00 0.00

0.02 0.00 0.09 0.00 0.00 0.17

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

3.24 0.05 12.52 0.26 0.01 22.93

0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00

Acuttl RQ'" '" (mg allkg.bw/day) , LD50

Acute RQ • mg ai n·2 I(LD50*bw)

Chronic RQ '" mg kg·1 saed I NOEC

48
Appendix F: Terr-Plant Modeling for Pyraclostrobin used as an encapsulation and as a

seed treatment:

49
Table 1. ChemiealldentitY.

Chemical Name IPyraclostrobin

PC code 99100

Use Corn

Application Method Encapsulation

Application Form Spray

:SOlUbility In water (ppm) 2.41

ITable 2. Input parameters used to derive EECs. I

Input Parameter Symbol Value Units

Application Rate A 0.1 Ibs ailA

Incorporation I 1 none

Runoff Fraction R 0.02 I none

Drift Fraction D 0.05 none

Table 3. EECs for Pvraclostrobin. Units in Ibs ailA.

Description Equation EEC

Runoff to dry areas (A1I)*R 0.002

Runoff to semi-aquatic areas (A1I)*R*10 0.02

Spray drift A*D 0.005

Total for dry areas «A1I)*R)+(A*D) 0.007

Total for semi-aquatic areas ((A1I)*R*101+(A*DI 0.025

Table 4. Plant .urvival and growth data used for RQ derivation. Units are in Ibs ailA.

Seedling Emergence Vegetative Vigor

Plant type EC25 NOAEC EC25 NOAEC

Monocot 0.78 0.3 0.6 0.6

Dicot 0.15 0.075 0.44 0.038

Table 5. RQ values for plants in dry and semi-aquatic areas exposed to Pyraelostrobin through runoff

andlor spray drift.*

Plant Type Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1

Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 0.17 <0.1

Dicot listed <0.1 0.33 <0.1

1*lfRQ > 1.0, the LOC IS exceeded, resulting In potential for risk to that plant group.

50
T.ble 1. ChemIcaIlcIentitY.

Chemical Name pyraclostrobin

PC code 99100

Use Sweet Corn

Application Method ground incorporation

Application Form liquid

SOlubility In Water (ppm) 2.41

T.ble2.ln '..... !:_.

Input Parameter Symbol Value Units

Application Rate A 0.0034 Ibs ai/A

Incorporation I 3 none

Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none

nble 3. ESC_ fer lWfIotoetro...... Unitt In .... • UA.

Description Eauation EEC

Runoff to dry areas (AlI)*R 1.13333E-05

Runoff to semi-aquatic areas (AlI)*R*10 0.000113333

Spray drift A*D 0.000034

Total for dry areas «AlI)*R)+{A*D) 4.53333E-05

Total for semi-aauatic areas ((AlI)*R*10)+(A*D) 0.000147333

rr..... 4. Plant"'l'YiWll ana ","for ,VatII 'N'".-..IIA

Seedling Emergence Vegetative Vigor

Plant type EC25 NOAEC EC25 NOAEC

Monocot 0.118 0.118 0.118 0.118

Dicot 0.118 0.118 0.118 0.118

T.b....RaV......,.,••in dry and semi-aqu.tIc .......Qosed to pyrac!oetrobln through "lnoff

land/or..naY ddft.·

Plant Type Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1

Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1

Dicot listed <0.1 <0.1 <0.1

I*If RQ > 1.0, the LOC IS exceeded, resulting In potential for risk to that plant group.

51
lTable 1. Chemical IdentitY.

Chemical Name pyraclostrobin

PC code 99100

Use Sugar beet seeds

Application Method Iground incorporation

Application Form liquid

SOlubility In water (ppm) 2.41

Table 2. InDut Dara.....used todetiveEEGe.

Input Parameter Svmbol Value Units

Application Rate A 0.1056 Ibs ailA

Incorporation I 1 none

Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none

Table 3. EECs for IM1lclosU'O,"n. Un" in Ibs aiiA.

Description Equation EEC

Runoff to dry areas (AlI)*R 0.001056

Runoff to semi-aquatic areas (AlI)*R*10 0.01056

Spray drift A*D 0.001056

Total for dry areas «A1I)*R)+(A*D) 0.002112

Total for semi-aquatic areas llAlI)*R*10)+(A*D) 0.011616

Table 4. P..nt ....Afiv.land lIJ'OWth .ata ..... fOrRG dertvaaon. u..i....in .... li#.A.

Seedling Emergence Vegetative Vigor

Plant type EC25 NOAEC EC25 NOAEC

Monocot 0.118 0.118 0.118 0.118

Dicot 0.118 0.118 0.118 0.118

Table 5. RClVJIUM for plan. indryand ..mf-aquatlC ....exposectto pyta8....................

anctlouprav drift.*

Plant Type Listed Status Dry Semi·AQuatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1

Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1

Dicot listed <0.1 <0.1 <0.1

I*If RQ > 1.0, the LOC IS exceeded, resulting In potential for risk to that plant group.

52
Tlble 1. ChemlCllldentltv.

Chemical Name pyraclostrobin

PC code 99100

Use Rapeseeds

Application Method Iground incorporation

Application Form liquid

SOlubility In Water (ppm) 2.41

lTabie I. InDut ............ ueecI to eMrlVeEEC••

Input Parameter Svmbol Value Units

Application Rate A 0.0096 Ibs ailA

Incorporation I 1 none

Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none

IT.b.. 3. .pyr.cIq$trobln. un'" In I• •UA.

Description Equation EEC

Runoff to dry areas (AlI)*R 0.000096

Runoff to semi-aquatlc areas (AlI)*R*10 0.00096

Spray drift A*D 0.000096

Total for dry areas «AlI)*R)+(A*D) 0.000192

Total for semi-aQuatic areas ((AlI)*R*10)+(A*D) 0.001056

TID" 4. Pllnt lurvlvalanet llrowth dl. ueecI for RCfderiVation. Un'" are In lbe aUA.

Plant type

Seedling Emergence Vegetative Vigor

EC25 NOAEC EC25 NOAEC

Monocot 0.118 0.118 0.118 0.118

Dicot 0.118 0.118 0.118 0.118

Tlble S.RQ Yalu..for pllnts in dty and aeml-aquatic are.. exposed to pyraciOllrobin through runoff

and/or IDI'8Y drift.*

Plant TVDe Listed Status Drv Semi·Aauatic SDrav Drift

Monocot non-listed <0.1 <0.1 <0.1

Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1

Dicot listed <0.1 <0.1 <0.1

I*If RQ > 1.0, the LOC IS exceeded, resulting In potential for risk to that plant group.

53
Appendix G: Ecotoxicological and Toxicological Summary

54
Table G-l. Acute Toxic Effects of Pyraclostrobin on Freshwater and Estuarine Fish.

Species Study % Toxicity Endpoint Toxicity Study MRID#

Guideline A.I. (Confidence Classification Classificati

Interval) on

Freshwater Fish

Bluegill Sunfish 72-la 97 LC,o 96 hr : 11.4 (9.52- Very highly toxic Supplemental 45118414

(Lepomis 15.9) ug a.i./L

macrochirus) NOAEC : 6.08 ug a.i. /L

(mortality and

symptoms)

Rainbow Trout 72-lc 97 LC,o 96 hr : 6.2 (5.25- Very highly toxic Acceptable 45118413

(Oncorhynchus 8.97) ug a.i. /L

mykiss) NOAEC : 3.60 ug a.i. /L

(mortality and

discoloration)

EstuarinelMarine Fish

Sheepshead Minnow 72-3a 97 LC,o 96 hr : 76.9 (53.5- Very highly toxic Supplemental 45118415

iCyprinodon 87.9) ug a.i. /L

variegatus) NOAEC : 53.5 /lg a.i./L

(survival)

Table G-2 Acute Toxic Effects of Pyraclostrobin degradation products on rainbow trout (Oncorhynchus mykiss).

Nameof Study %A.I. Toxicity Endpoint Toxicity Study MRID#

transformation Product Guideline (Confidence Interval) Classification Classification

BF 500-11 72-lc 99 LC,o 96 hr :>99.18 mg a.i. Practically non- Acceptable 45118416

(Reg. No. 411847) /L toxic

NOAEC : 9.98 mg a.i. /L

(apathy etc.)

BF 500-13 72-lc 100 LC,o 96 hr :75 mg a.i./L Slightly toxic Acceptable 45118417

(Reg. No. 412785) NOAEC : 9.3 mg a.i. /L

(convulsions etc.)

BF 500-14 72-lc 96 LC,o 96 hr :57 mg a.i./L Slightly toxic Acceptable 45118418

(Reg. No. 413038) NOAEC : 39.4 mg a.i. /L

(mortality)

55
Table G-3 Chronic Toxicity Effects ofPyraclostrobin in Fish.

Species Study % A.I. Toxicity Endpoint Study MRID#

Guideline (Confidence Interval) Classification

Freshwater Fish

Fathead Minnow 72-4a 97.1 NOAEC: 4.14 ug a.i. /L Acceptable 45118420

(Pimephales promelas) LOAEC: 8.37 ug a.i. /L

(hatch/post hatch survival,

bodyweight and length and sublethal

symptoms)

Rainbow Trout 72-4a 99 NOAEC: 2.35 ug a.i. /L Supplemental 45118421

(Oncorhynchus mykiss) LOAEC: 6.42 ug a.i. /L

(hatch/post hatch survival)

Estuarine/Marine Fish

Sheepshead Minnow 72-4a 93.5 NOAEC: 10.8 ~g a.i. /L Acceptable 45420101

(Cyprinodon variegatus) LOAEC: 24.0 ug a.i. /L

(hatch/post hatch survival)

Table G-4. Acute and Chronic Toxic Effects of Pyraclostrobin in Aquatic Invertebrates.

Species Study % A.I. Toxicity Endpoint Toxicity Study MRID#

Guideline (Confidence Interval Classification' Classification

Freshwater Invertebrates

Daphnia magna 72-2 97.1 EC50 48 hr: 15.7 a.i. (14.2- Very highly toxic Supplemental 45118407

17.4) ug/L

NOAEC: 11.5 ug a.i. /L

(immobility)

Daphnia magna 72-4b 97.1 NOAEC : 4 ug a.i. /L - Supplemental 45118419

LOAEC: 8 ug a.i. /L

(Number ofoffsprings

produced, length and survival

of daphnids)

EstuarinelMarine Invertebrates

Eastern Oyster 72-3b 97.1 EC50 96 hr : 12.5 a.i.(9.2- Very highly toxic Acceptable 45118411

(Crassostrea 17.1)

virginica) ~g/L

Mollusk Shell NOAEC : 4.06 ug a.i./L

Deposition (shell deposition)

Mysid Shrimp 72-3c 97.1 LC50 96 hr : 4.16 (3.64-5.97) Very highly toxic Acceptable 45118412

(Americamysis ug a.i./L

bahia) NOAEC : 2.12 ug a.i./L

(lethargy and mortality)

Table G-5. Acute Toxicity Studies of the transformation Product of Pyraclostrobin in Daphnia magna.

56
Name of Study %A.I. Toxicity Endpoint Toxicity Study MRID#

transfonnation Guideline (Confidence Interval) Classification Classification

Product

BF 500-11 72-2 98.9 ECso 48 hr : >100 Practically non- Supplemental 45118408

mg/L toxic

NOEC: 100 mg/L

(immobility)

BF 500-13 72-2 99.6 ECso48 hr : >I00 Practically non- Supplemental 45118409

mg/L toxic

NOEC : 50 mg/L

(immobility)

BF 500-14 72-2 96.1 ECso 48 hr: >61.8 Slightly non-toxic Supplemental 45118410

mg/L

NOEC: 2':61.8 mg/L

(immobility)

57
Table G-6. Acute Toxic Effects of Pyraclostrobin on Aquatic Plants.

Species Study 0/0 Toxicity Endpoint Study MRID#

Guideline A.l. (Confidence Interval Classification

Nonvascular Plants

Freshwater Green Algae 123-2 97.1 ECso 96 hr: 152 (143-162) ug a.i.1L Acceptable 45118722

(Pseudokirehnerie//a (biomass)

subeapitata) NOAEC: 14.0 ug a.iJL

(Biomass and Number of cells/ml)

Freshwater Blue-Green 123-2 97.1 ECso 120 hr : > 1780 ug a.i.IL Acceptable 45118716

Algae (No. of celIs/ml and growth rate)

(Anabaena fios-aquae) NOAEC : 1780 ug a.i.1L

(Growth rate and Number of celIs/m1)

Freshwater diatoms 123-2 97.1 ECso 120 hr: 1.50 «118 - 3.83) ug a.i.IL Acceptable 45118721

(Navieu/a pel/ieu/osa) (No. of celIs/ml)

NOAEC: 1.18 J.lg a.i./L

(Number of cells/ml and Growth rate)

Marine diatoms 123-2 97.1 ECso 120 hr : 64.7 (50 - 84) ug a.i.1L (No. Acceptable 45118717

(Ske/etonema eostatum) of cells/ml)

NOAEC: 10.0 ug a.i.1L

(Number of cells/ml)

Vascular Plant

Duckweed 123-2 97.1 ECso 14 days: 1720 ug a.ilL Acceptable 45118715

Lemna gibba (Plant biomass)

NOAEC : 896 J.lg a.i./L

(Number of normal fronds and plant

biomass)

58
Table G-7. The Toxicity of the Transformation Products ofPyraclostrobin in Freshwater Algae (Scenedesmus subspicatus).

Name of Study %A.I. Toxicity Endpoint Study MRID#

transformation Guideline (Confidence Interval) Classification

Product

BF 500-11 123-2 98.9 ECso72 hr: >1000,000 ug.a.i, /L Supplemental 45118718

(biomass; growth rate)

NOAEC : 50,000 ug a.i.lL

(Number of cells/ml)

BF 500-13 123-2 99.6 ECso72 hr : 66,000 ug.a.i, /L Supplemental 45118719

(biomass)

NOAEC : 12,500 ug a.i./L

(Number of cells/ml)

BF 500-14 123-2 96.1 ECso72 hr : 46,600 ug.a.i, /L Supplemental 45118720

(biomass)

NOAEC: 12,500 ug a.i./L

(Number of cells/ml)

Table G-8. Avian Toxicity Studies of Pyrac1ostrobin.

Species Study %A.I. Toxicity Endpoint Toxicity Study MRID#

Guideline (Confidence Interval) Classification Classification

Bobwhite quail 71-la 97.1 LDso 14 days: > 2000 Practically Acceptable 45118423

(Colinus mg/KgBW non-toxic

virginianusy NOAEC: 500 mg/kg BW

(body weight gain)

Bobwhite quail 71-2a 98.7 LCso8 days: > 5000 mg/Kg Practically Acceptable 45118424

(Colinus diet non-toxic

virginianusi NOAEC: 2500 mg/kg diet

(body weight gain)

Mallard duck 71-2b 97.1 LCso8 days : > 5000 mg/Kg Practically Acceptable 45118425

(Anas diet non-toxic

platyrhynchos) NOAEC: 625 mg/kg diet

(body weight gain)

Bobwhite quail 71-4a 98.7 NOAEC: 1061.5 mg/kg diet N/A Acceptable 45118426

(Colinus

virginianus)

Mallard duck 71-4b 98.7 NOAEC: 1062 mg a.i./kg N/A Acceptable 45118427

(Anas diet

platyrhynchos)

59
Table G-9 Mammalian Toxicity Studies of Pyraclostrobin.

Species Study 0/0 Toxicity Endpoint Toxicity Study MRID#

Guidelin A.I. (Confidence Interval) Classificati Classificati

e on on

Rats 81-1 98.5 LDso: 25000 mg/kg-bw (males and Category IV Acceptable 45118302

females) (Low

toxicity)

Rats 81-1 23.4 LDso: 500-2000 mg/kg-bw (males) Category 11 Acceptable 45118303

(Headline LDso: 260 mg/kg-bw (females) (High

EC) LDso: 500 mg/kg-bw (males and toxicity)

females)

Rats 81-1 21 LDso: >2000 mg/kg-bw (males and Category 111 Acceptable 45118304

(Cabrio females) (Low

EG) toxicity)

Table G-IO. Chronic Mammalian Toxicity Studies ofPyraclostrobin.

Species Study 0/0 Toxicity Endpoint Study Classification MRID#

Guideline A.I. (Confidence Interval)

Rats 83-3a 98.9 Maternal: Acceptable-Guideline 45118325

INOAEL: 10 mg/kg/day

lLOAEL 25 mg/kg/day

Developmental

2NOAEL: 25 mg/kg/day

2LOAEL: 50 mglkg/day

Rabbits 83-3b 98.9 Maternal: Acceptable-Guideline 45118326

1 NOAEL:5 mg/kg/day

I LOAEL 10 mglkg/day

Developmental

3 NOAEL: 5 mg/kg/day

3 LOAEL: 10 mglkg/day

Rats 83-4 98.7 NOAEL 2300 ppm Acceptable-Guideline 45118327

Two generation reproduction study

(29-35 mglkgIBW)

decreased body weight gam and reduced food consumption, reduced food efficiency.

2Increase in fetal and litter incidence of dilated renal pelvis; increased incidence of cervical ribs with cartilage not

present.

3 Increased resorptions per litter, increased post implantation loss and dams with total resorptions.

60
Table G-ll. Non-target Insect Toxicity Studies of Pyraclostrobin.

Species Study 0/0 Toxicity Endpoint Toxicity Study MRID#

Guideline A.I. (Confidence Classification Classification

Interval)

Honey 141-1 97.09 LDso 48 hr: > 100 ug Practically Acceptable 45118711

bees a.i./bee nontoxic

(Apis NOEC : :::-: 100 I!g

mellifera) a.i./bee

(survival)

Table G-12. Terrestrial Plant Toxicity Studies of Pyraclostrobin.

Species Study 0/0 Toxicity Endpoint Study MRID#

Guideline A.I. (Confidence Interval) Classification

Seedling Emergence, 122-1a 23.6 EC25 21 days Supplemental 45118713

Tier 1 (Monocots and Dicots):

(Monocots and Dicots) > 0.118 lb a.i. /acre

(percent emergence, percent

survival, phytotoxicity rating,

plant height and dry weight)

NOEC : :::-: 0.118 lb a.i. /acre

(Same parameters as above)

Vegetative Vigor 122-1b 23.6 EC2s 21 days Supplemental 45118714

Tier I (Monocots and Dicots):

(Monocots and Dicots) > 0.1181b a.i. /acre

(Same parameters as in

Emergence)

NOEC::::-: 0.1181b a.i. /acre

(Same parameters as in

Emergence)

61
Appendix H: LOCATES Runs

62
Species Counts by State for Indicated Crops

No species were excluded.

Minimum of 1 Acre.

All Medium Types Reported

corn (PR), cornfor grain, cornfor grain (irrigated), cornfor silage or

greenchop, corn for silage or greenchop (irrigated), Sugar beet seeds, Sweet

Corn seeds, and Rapeseeds

AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

Alabama

The taxa Amphibian has 2 species co-occurring with indicated crops.

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Bivalve has 30 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 10 species co-occurring with indicated crops.

The taxa Ferns has 3 species co-occurring with indicated crops.

The taxa Fish has 15 species co-occurring with indicated crops.

The taxa Gastropod has 10 species co-occurring with indicated crops.

The taxa Mammal has 4 species co-occurring with indicated crops.

The taxa Monocot has 3 species co-occurring with indicated crops.

The taxa Reptile has5 species co-occurring with indicated crops.

Arizona

The taxa Amphibian has 2 species co-occurring with indicated crops.

The taxa Bird has 8 species co-occurring with indicated crops.

The taxa Dicot has 14 species co-occurring with indicated crops.

The taxa Fish has 15 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Mammal has 8 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 2 species co-occurring with indicated crops.

63
Arkansas

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 6 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Fish has 3 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

California

The taxa Amphibian has 6 species co-occurring with indicated crops.

The taxa Bird has 13 species co-occurring with indicated crops.

The taxa Conf/cycds has 1 species co-occurring with indicated crops.

The taxa Crustacean has 8 species co-occurring with indicated crops.

The taxa Dicot has 136 species co-occurring with indicated crops.

The taxa Fish has 25 species co-occurring with indicated crops.

The taxa Gastropod has 2 species co-occurring with indicated crops.

The taxa Insect has 15 species co-occurring with indicated crops.

The taxa Mammal has 20 species co-occurring with indicated crops.

The taxa Marine mml has 2 species co-occurring with indicated crops.

The taxa Monocot has 15 species co-occurring with indicated crops.

The taxa Reptile has 7 species co-occurring with indicated crops.

Colorado

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Dicot has 8 species co-occurring with indicated crops.

The taxa Fish has 5 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Connecticut

The taxa Bird has 2 species co-occurring with indicated crops.

AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

64
VA,

WA, WV, WI, WY

The taxa Bivalve has 1 species co-occurring with indicated crops.

The taxa Dicot has 1 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

Delaware

The taxa Bird has 1 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

Florida

The taxa Amphibian has 2 species co-occurring with indicated crops.

The taxa Bird has 8 species co-occurring with indicated crops.

The taxa Bivalve has 7 species co-occurring with indicated crops.

The taxa Conf/cycds has 1 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 43 species co-occurring with indicated crops.

The taxa Fish has 4 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Lichen has 1 species co-occurring with indicated crops.

The taxa Mammal has 8 species co-occurring with indicated crops.

The taxa Marine mml has 1 species co-occurring with indicated crops.

The taxa Monocot has 3 species co-occurring with indicated crops.

The taxa Reptile has 10 species co-occurring with indicated crops.

Georgia

The taxa Amphibian has 2 species co-occurring with indicated crops.

AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

65
VA,

WA, WV, WI, WY

The taxa Bird has 4 species co-occurring with indicated crops.

The taxa Bivalve has 16 species co-occurring with indicated crops.

The taxa Conf/cycds has 1 species co-occurring with indicated crops.

The taxa Dicot has 11 species co-occurring with indicated crops.

The taxa Ferns has 2 species co-occurring with indicated crops.

The taxa Fish has 11 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

The taxa Marine mml has 1 species co-occurring with indicated crops.

The taxa Monocot has 6 species co-occurring with indicated crops.

The taxa Reptile has 2 species co-occurring with indicated crops.

Hawaii

The taxa Arachnid has 1 species co-occurring with indicated crops.

The taxa Bird has 28 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 216 species co-occurring with indicated crops.

The taxa Ferns has 11 species co-occurring with indicated crops.

The taxa Gastropod has 39 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Marine mml has 1 species co-occurring with indicated crops.

The taxa Monocot has 21 species co-occurring with indicated crops.

The taxa Reptile has 2 species co-occurring with indicated crops.

Idaho

The taxa Bird has 1 species co-occurring with indicated crops.

The taxa Dicot has 2 species co-occurring with indicated crops.

The taxa Fish has 8 species co-occurring with indicated crops.

The taxa Gastropod has 4 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

66
AL, AK, AZ.., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN,MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

Illinois

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 7 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 7 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

Indiana

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 11 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

Iowa

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 2 species co-occurring with indicated crops.

The taxa Dicot has 3 species co-occurring with indicated crops.

The taxa Ferns has 1 species co-occurring with indicated crops.

The taxa Fish has 2 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

Kansas

The taxa Bird has 3 species co-occurring with indicated crops.

67
AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

The taxa Dicot has 1 species co-occurring with indicated crops.

The taxa Fish has 4 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Kentucky

The taxa Bird has 6 species co-occurring with indicated crops.

The taxa Bivalve has 22 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 10 species co-occurring with indicated crops.

The taxa Fish has 5 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

Louisiana

The taxa Bird has 4 species co-occurring with indicated crops.

The taxa Bivalve has 3 species co-occurring with indicated crops.

The taxa Dicot has 2 species co-occurring with indicated crops.

The taxa Ferns has 1 species co-occurring with indicated crops.

The taxa Fish has 2 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Marine mml has 1 species co-occurring with indicated crops.

The taxa Reptile has 7 species co-occurring with indicated crops.

Maine

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Dicot has 1 species co-occurring with indicated crops.

The taxa Fish has 2 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

68
AL, AK, AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, ID, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, ND, OH, OK, OR, PA, PR, RI, SC, SD, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

Maryland

The taxa Bird has 1 species co-occurring with indicated crops.

The taxa Bivalve has 1 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Fish has 2 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

Massachusetts

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Dicot has 1 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Insect has 3 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 2 species co-occurring with indicated crops.

Michigan

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 2 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Ferns has 1 species co-occurring with indicated crops.

The taxa Insect has 4 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 3 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

Minnesota

The taxa Bird has 1 species co-occurring with indicated crops.

The taxa Bivalve has 2 species co-occurring with indicated crops.

The taxa Dicot has 2 species co-occurring with indicated crops.

69
AL, AK, AZ.., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

Mississippi

The taxa Amphibian has 1 species co-occurring with indicated crops.

The taxa Bird has 4 species co-occurring with indicated crops.

The taxa Bivalve has 9 species co-occurring with indicated crops.

The taxa Dicot has 2 species co-occurring with indicated crops.

The taxa Ferns has 1 species co-occurring with indicated crops.

The taxa Fish has 3 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

The taxa Reptile has 7 species co-occurring with indicated crops.

Missouri

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 6 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 7 species co-occurring with indicated crops.

The taxa Fish has 7 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Montana

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Dicot has 2 species co-occurring with indicated crops.

The taxa Fish has 4 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

70
AL, AK, AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, ID, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, ND, OH, OK, OR, PA, PR, RI, SC, SD, TN, TX, UT, VT,

VA,

WA,WV, WI, WY

Nebraska

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Dicot has 2 species co-occurring with indicated crops.

The taxa Fish has 2 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Nevada

The taxa Dicot has 8 species co-occurring with indicated crops.

The taxa Fish has 16 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

New Hampshire

The taxa Bivalve has 1 species co-occurring with indicated crops.

The taxa Dicot has 1 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

New Jersey

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Dicot has 2 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 3 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

New Mexico

The taxa Amphibian has 1 species co-occurring with indicated crops.

The taxa Bird has 6 species co-occurring with indicated crops.

71
The taxa Crustacean has 2 species co-occurring with indicated crops.

AL, AK, AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

The taxa Dicot has 11 species co-occurring with indicated crops.

The taxa Fish has 13 species co-occurring with indicated crops.

The taxa Gastropod has 5 species co-occurring with indicated crops.

The taxa Mammal has 4 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

New York

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 1 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Ferns has 1 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

North Carolina

The taxa Arachnid has 1 species co-occurring with indicated crops.

The taxa Bird has 4 species co-occurring with indicated crops.

The taxa Bivalve has 8 species co-occurring with indicated crops.

The taxa Dicot has 21 species co-occurring with indicated crops.

The taxa Fish has 4 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Lichen has 1 species co-occurring with indicated crops.

The taxa Mammal has 4 species co-occurring with indicated crops.

The taxa Marine mml has 1 species co-occurring with indicated crops.

The taxa Monocot has 5 species co-occurring with indicated crops.

72
The taxa Reptile has 5 species co-occurring with indicated crops.

AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

North Dakota

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Ohio

The taxa Bird has 1 species co-occurring with indicated crops.

The taxa Bivalve has 6 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Insect has 4 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 2 species co-occurring with indicated crops.

Oklahoma

The taxa Bird has 6 species co-occurring with indicated crops.

The taxa Bivalve has 2 species co-occurring with indicated crops.

The taxa Fish has 4 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

Oregon

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 9 species co-occurring with indicated crops.

The taxa Fish has 22 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

73
74
AL, AK, AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MO, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

Pennsylvania

The taxa Bird has 1 species co-occurring with indicated crops.

The taxa Bivalve has 2 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

Puerto Rico

The taxa Amphibian has 3 species co-occurring with indicated crops.

The taxa Bird has 6 species co-occurring with indicated crops.

The taxa Oicot has 28 species co-occurring with indicated crops.

The taxa Ferns has 7 species co-occurring with indicated crops.

The taxa Marine mml has 1 species co-occurring with indicated crops.

The taxa Monocot has 4 species co-occurring with indicated crops.

The taxa Reptile has 7 species co-occurring with indicated crops.

Rhode Island

The taxa Bird has 1 species co-occurring with indicated crops.

The taxa Oicot has 1 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

South Carolina

The taxa Amphibian has 1 species co-occurring with indicated crops.

The taxa Bird has 4 species co-occurring with indicated crops.

The taxa Bivalve has 1 species co-occurring with indicated crops.

The taxa Oicot has 12 species co-occurring with indicated crops.

The taxa Ferns has 1 species co-occurring with indicated crops.

The taxa Fish has 1 species co-occurring with indicated crops.

The taxa Lichen has 1 species co-occurring with indicated crops.

75
The taxa Mammal has 1 species co-occurring with indicated crops.

76
AL, AK, AZ., AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PR, RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

The taxa Marine mml has 3 species co-occurring with indicated crops.

The taxa Monocot has 6 species co-occurring with indicated crops.

The taxa Reptile has 5 species co-occurring with indicated crops.

South Dakota

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Fish has 2 species co-occurring with indicated crops.

The taxa Insect has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Tennessee

The taxa Arachnid has 1 species co-occurring with indicated crops.

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Bivalve has 38 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 16 species co-occurring with indicated crops.

The taxa Ferns has 1 species co-occurring with indicated crops.

The taxa Fish has 16 species co-occurring with indicated crops.

The taxa Gastropod has 3 species co-occurring with indicated crops.

The taxa Lichen has 1 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

Texas

The taxa Amphibian has 4 species co-occurring with indicated crops.

The taxa Arachnid has 10 species co-occurring with indicated crops.

The taxa Bird has 11 species co-occurring with indicated crops.

The taxa Crustacean has 1 species co-occurring with indicated crops.

The taxa Dicot has 26 species co-occurring with indicated crops.

The taxa Fish has 7 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

77
AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, ID, IL, IN, IA, KS, KY, LA, ME, MD, MA, MI,

MN, MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, ND, OH, OK, OR, PA, PR, RI, SC, SD, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

The taxa Insect has 9 species co-occurring with indicated crops.

The taxa Mammal has 5 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 6 species co-occurring with indicated crops.

Utah

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Dicot has 19 species co-occurring with indicated crops.

The taxa Fish has 8 species co-occurring with indicated crops.

The taxa Mammal has 2 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

Vermont

The taxa Bivalve has 1 species co-occurring with indicated crops.

The taxa Dicot has 1 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Virginia

The taxa Amphibian has 1 species co-occurring with indicated crops.

The taxa Bird has 2 species co-occurring with indicated crops.

The taxa Bivalve has 21 species co-occurring with indicated crops.

The taxa Crustacean has 2 species co-occurring with indicated crops.

The taxa Dicot has 13 species co-occurring with indicated crops.

The taxa Fish has 7 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Insect has 3 species co-occurring with indicated crops.

The taxa Mammal has 4 species co-occurring with indicated crops.

The taxa Monocot has 4 species co-occurring with indicated crops.

The taxa Reptile has 1 species co-occurring with indicated crops.

78
AL, AK, AZ, AR, CA, CO, CT, DE, DC, FL, GA, HI, 10, IL, IN, lA, KS, KY, LA, ME, MD, MA, MI,

MN,MS,

MO, MT, NE, NV, NH, NJ, NM, NY, NC, NO, OH, OK, OR, PA, PRo RI, SC, SO, TN, TX, UT, VT,

VA,

WA, WV, WI, WY

Washington

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Dicot has 7 species co-occurring with indicated crops.

The taxa Fish has 19 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

West Virginia

The taxa Amphibian has 1 species co-occurring with indicated crops.

The taxa Bivalve has 5 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Gastropod has 1 species co-occurring with indicated crops.

The taxa Mammal has 4 species co-occurring with indicated crops.

The taxa Monocot has 1 species co-occurring with indicated crops.

Wisconsin

The taxa Bird has 3 species co-occurring with indicated crops.

The taxa Bivalve has 2 species co-occurring with indicated crops.

The taxa Dicot has 4 species co-occurring with indicated crops.

The taxa Insect has 2 species co-occurring with indicated crops.

The taxa Mammal has 1 species co-occurring with indicated crops.

The taxa Monocot has 2 species co-occurring with indicated crops.

Wyoming

The taxa Dicot has 2 species co-occurring with indicated crops.

The taxa Mammal has 3 species co-occurring with indicated crops.

No species were excluded,

Dispersed species included in report.

79