# Patent

## Title
Anhydrous polymorphs of [(2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)} methyl nitrate and processes of preparation thereof

## Abstract

The present invention provides novel anhydrous polymorph forms of 2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl) methyl nitrate (Compound A), a selective adenosine A1 receptor agonist with a number of therapeutic uses including the treatment of elevated intra-ocular pressure. Also provided are methods for the preparation of the anhydrous polymorphic forms of compound A, pharmaceutical compositions and methods of treatment.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims priority to U.S. Provisional Application No. 61/591,037, filed Jan. 26, 2012. The entire contents of the aforementioned application and any patents, patent applications, and references cited throughout this specification are herein incorporated by reference in their entireties.


FIELD OF THE INVENTION
The present invention provides novel anhydrous polymorph forms of [2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)] methyl nitrate (Compound A) and to processes of preparation thereof.
BACKGROUND OF THE INVENTION
Compound A is represented by the following structure




    [2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)] methyl nitrate,


Compound A is a selective adenosine A1 receptor agonist and is of particular use in the treatment of elevated intra-ocular pressure as described in PCT/US2010/033112 (published as WO2010/127210).
Compound A can be prepared using the procedures described in U.S. Pat. No. 7,423,144, US 20090062314, and WO2010/127210 all of which are herein incorporated by reference in their entirety.
Many pharmaceutical solids can exist in different physical forms. Polymorphism can be characterized as the ability of a drug substance to exist in two or more crystalline phases that have different arrangements and/or conformations of the molecules in the crystal lattice.
Polymorphs of a pharmaceutical solid can have different physical and solid state chemical properties. The most stable polymorphic form of a drug substance is often used because it has the lowest potential for conversion from one polymorphic form to another.
A particular crystalline form of a compound can have physical properties that differ from those of other polymorphic forms and such properties can influence the physico-chemical and pharmaceutical processing of the compound, particularly when the compound is prepared or used on a commercial scale. Such differences may alter the mechanical handling properties of the compound, such as dispersion in a blend of solid formulation excipients or within a suspension formulation. Polymorphs are also known in some cases to have different chemical stability profiles and different solubility of the solid material. As a result of these potential polymorph-specific physiochemical differences, the discovery of new polymorphic forms provides a new opportunity to improve the manufacturing or characteristics of a pharmaceutical end product.
Further, new polymorphic forms of a drug substance can display different melting point, hygroscopicity, stability, solubility and/or dissolution rate, crystallinity, crystal properties, and formulation handling characteristics, which are among the numerous properties that need to be considered in preparing medicament that can be effectively administered, they can materially impact the quality of a pharmaceutical product. Furthermore, regulatory agencies require a definitive knowledge, characterization and control of the polymorphic form of the active component in pharmaceutical dosage forms if it is in the solid state.
Compound A is under development by the Applicants for reducing intraocular pressure. The Applicants have found a number of polymorphs of Compound A that are useful for controlling certain desirable formulation properties. In particular two anhydrous forms have been identified, isolated and characterized.
SUMMARY OF INVENTION
Provided herein are anhydrous polymorphs of Compound A, and methods of preparation thereof.
Thus, in a first aspect there is provided an isolated anhydrous polymorph A1 of Compound A having the following crystal data,
C15H20N6O6;
Mr=380.37;
Monoclinic crystal system;
P21 space group;
a=5.546(2) Å;
b=7.107(2) Å;
c=21.929(9) Å;
V=858.8(5) Å3, and
Z=2.
In another aspect there is provided an isolated polymorph A1 of Compound A having substantially equivalent peaks at a reflection angle 2-theta as shown in Table 3.
In one embodiment there is provided an isolated polymorph of form A1 as defined above that is at least about 75% free of other polymorphic forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 80% free of other polymorphic forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 90% free of other polymorphic forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 95% free of other polymorphic forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 99% free of other polymorphic forms.
In one embodiment there is provided an isolated polymorph as defined above that is 100% free of other forms.
In another aspect there is provided a method of obtaining the polymorph A1, the method comprising the steps of taking Compound A and recrystallizing from ethanol.
In another aspect there is provided a method of obtaining the polymorph A1, the method comprising the steps of taking Compound A and recrystallizing from isopropanol, ethyl acetate, or isopropyl acetate.
In another aspect there is provided a method of obtaining the polymorph A1, the method comprising the steps of taking Compound A and recrystallizing from 1,4 dioxane, 2-methoxy ethanol, 3-methyl-2-butanone, methylethyl ketone, or 1,2-dimethoxyethane.
In another aspect there is provided a pharmaceutical composition comprising polymorph A1 as defined above and further comprising one or more pharmaceutically acceptable ingredients selected from the group consisting of carriers, excipients, diluents, additives, fillers, surfactants, binders, antimicrobial preservatives, viscosity enhancing agents, and buffers.
In one embodiment the pharmaceutical composition comprising polymorph A1 defined above is formulated for ophthalmic administration.
In a further aspect, there is also provided a method of treating a subject in need of a selective adenosine A1 agonist, the method comprising administering to a subject in need thereof a therapeutically effective amount of the polymorph A1 defined above.
In a further aspect, there is also provided a method of reducing intraocular pressure in a subject, the method comprising topically administering to an eye of a subject in need thereof a therapeutically effective amount of the polymorph A1 defined above.
Thus, in another aspect there is provided a polymorph A2 of Compound A having the following crystal data,
C15H20N606;
Mr=380.37;
Orthorhombic crystal system;
P212121 space group;
a=5.51796(17) Å;
b=7.14615(29) Å;
c=42.9738(29) Å and
V=1694.55(14) Å3.
Thus, in another aspect there is provided a polymorph A2 of Compound A having substantially equivalent peaks at a reflection angle 2-theta as shown in Table 5.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 75% free of other forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 80% free of other forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 90% free of other forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 95% free of other forms.
In one embodiment there is provided an isolated polymorph as defined above that is at least about 99% free of other forms.
In one embodiment there is provided an isolated polymorph as defined above that is 100% free of other forms.
In another aspect there is provided a method of obtaining the polymorph A2, the method comprising the steps of taking Compound A in a liquid vehicle and heating up to about 40 degrees for at least 9 hours.
In one embodiment the Compound A is micronized and then added to an aqueous liquid vehicle. In one embodiment Compound A is micronized into particles with sizes less than 50 microns.
In one embodiment the method includes the step of heating to about 40 degrees C. for 15 hours.
In one embodiment the liquid vehicle is adapted to provide an aqueous suspension of Compound A. In another embodiment the liquid vehicle includes a surfactant and a preservative. In one embodiment the surfactant is selected from polysorbate 80, polysorbate 60, polysorbate 40, polysorbate 20, polyoxyl 40 stearate, poloxamers, tyloxapol, POE 35 and castor oil. In one embodiment the preservative in selected from a quaternary ammonium salt, benzalkonium chloride, cetrimide, chlorobutanol, sorbic acid and boric acid.
In another aspect there is provided a pharmaceutical composition comprising polymorph A2 as defined above and further comprising one or more pharmaceutically acceptable ingredients selected from the group consisting of carriers, excipients, diluents, additives, fillers, surfactants, binders, antimicrobial preservatives, viscosity enhancing agents, and buffers.
In one embodiment the pharmaceutical composition comprising polymorph A2 defined above is formulated for ophthalmic administration.
In a further aspect, there is also provided a method of treating a subject in need of a selective adenosine A1 agonist, the method comprising administering to a subject in need thereof a therapeutically effective amount of the polymorph A2 defined above.
In a further aspect, there is also provided a method of reducing intraocular pressure in a subject, the method comprising topically administering to an eye of a subject in need thereof a therapeutically effective amount of the polymorph A2 defined above.
The foregoing brief summary broadly describes the features and technical advantages of certain embodiments of the present invention. Further technical advantages will be described in the detailed description of the invention that follows. Novel features which are believed to be characteristic of the invention will be better understood from the detailed description of the invention when considered in connection with any accompanying figures and examples. However, the figures and examples provided herein are intended to help illustrate the invention or assist with developing an understanding of the invention, and are not intended to be definitions of the invention's scope.



BRIEF DESCRIPTION OF THE DRAWINGS 
FIG. 1: shows the molecular structure and atom numbering scheme for Compound A—Form A1.
FIG. 2: shows the packing arrangement and H-bonds for Compound A—Form A1 crystals.
FIG. 3: shows the molecular structure and atom numbering scheme for Compound A—Form A2
FIG. 4: shows the packing arrangement and H-bonds for Compound A—Form A2 crystals.
FIG. 5: shows an overlay in the x-ray powder spectra observed for the forms of Compound A described herein. The lower gray line represents the A1 form and the upper black line represents the form A2.
FIG. 6: shows the superposition of molecules of Form A1 (black) and Form A2 (grey)
FIG. 7: shows the XRPD data plot of conversion of polymorph form A1 to polymorph form A2 over time at 40 degrees C.



DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention provide anhydrous polymorphs of Compound A.
Definitions
Some chemical structures herein are depicted using bold and dashed lines to represent chemical bonds. These bold and dashed lines depict absolute stereochemistry. A bold line indicates that a substituent is above the plane of the carbon atom to which it is attached and a dashed line indicates that a substituent is below the plane of the carbon atom to which it is attached.
The term “effective amount” as used herein refers to an amount of a selective adenosine A1 agonist that is effective for: (i) treating or preventing elevated IOP; or (ii) reducing IOP in a human.
The term “subject” is intended to include organisms, e.g., prokaryotes and eukaryotes, which are capable of suffering from or afflicted with a disease, disorder or condition associated with elevated IOP. Examples of subjects include mammals, e.g., humans, dogs, cows, horses, pigs, sheep, goats, cats, mice, rabbits, rats, and transgenic non-human animals. In certain embodiments, the subject is a human, e.g., a human suffering from, at risk of suffering from, or potentially capable of suffering from an increase in TOP. In another embodiment, the subject is a cell.
The term “treat,” “treated,” “treating” or “treatment” includes the diminishment or alleviation of at least one symptom associated or caused by the state, disorder or disease being treated. In certain embodiments, the treatment comprises the induction of elevated TOP, followed by the activation of the compound of the invention, which would in turn diminish or alleviate at least one symptom associated or caused by the elevated TOP. For example, treatment can be diminishment of one or several symptoms of a disorder or complete eradication of a disorder.
The term “about” or “substantially” usually means within 20%, more preferably within 10%, and most preferably still within 5% of a given value or range.
Methods of Preparation and Studies
Synthesis of Compound A
The following Scheme 1 shows the reaction scheme in the preparation of Compound A. The preparation of Compound A is described in detail.
The quantities detailed are calculated for a production batch of approximately 40 gms of Compound A. The production described can be scaled up.
Step 1: 1 Liter of ethanol was charged into a reactor and stirred rapidly. 0.3 kg of 6-chloroadenosine and 0.267 kg of cyclopentylamine were added to the ethanol in the reactor. The reactor was heated to reflux for 2 hr, then cooled to 8 degrees C. and kept under these conditions for 12 hours. The crystallized material was filtered from the mother liquid and the solid cake was washed with 0.33 L of ethanol to produced a wet cake. The wet cake was dried to obtain N6-cyclopentyladenosine (0.249 kg).
Step 2: Dimethoxypropane was used to protect the 2′ and 3′ hydroxyls on the sugar unit. 3.7 liters of acetone was charged into the reactor and was stirred rapidly. 0.249 kg of N6-cyclopentyladenosine; 0.386 kg of dimethoxypropane and 0.148 kg of p-toluenesulfonic acid were added to the acetone (3.7 L) in the reactor. The reactor was heated to 40 degrees C. for 1.5 hours. The solvents were then removed by distillation under vacuum at 40 degrees C. to prepare a dry crude material. 3.1 L of ethyl acetate were then added to the dry crude material obtained. The solution was then cooled to 6 degrees C. and 0.5N NaOH solution was added by dripping until a pH of 8 was reached. This equated to approximately 1.55 L of NaOH solution. After the phase separation was complete, 0.78 L of saturated sodium chloride 20% solution was added to the organic phase. 0.78 L of saturation sodium chloride 20% solution was added again. The two phases were stirred for 30 minutes. The organic phase that was ethyl acetate based was separated and dried with 0.157 kg of sodium sulfate and washed with 1 L of ethyl acetate. The solution was filtered and evaporated to an oil under vacuum at 55 degrees C. To the remaining oil 1.2 L of hexane and 0.3 L of ethyl acetate were added. The reaction mixture was heated to 55 degrees C. for 3 hours and then the solution was cooled to 5 degrees C. and maintained at this temperature for 12 hours. The solids were filtered and the resulting cake was washed with a 0.625 L of ethyl acetate:hexane (1:4) solution. After drying the solid 140 g of 2′,3′-isopropylidene-N6-cyclopentyl adenosine was obtained.
Step 3: Nitration of the 5′ position of 2′,3′-isopropylidene-N6-cyclopentyl adenosine obtained in Step 2 was carried out with a nitric acid acetic anhydride mixture. 0.127 L of dichloromethane was charged into the reactor and stirred rapidly. 140 g of 2′,3′-isopropylidene-N6-cyclopentyl adenosine was added and the reaction solution was cooled to −20 degrees C. 0.547 L of a solution composed of 0.127 L nitric acid 65% in 0.420 L of acetic anhydride was added at a rate that kept the reaction mixture below −15 degrees C.—the temperature range of between −23 to −18 degrees C. has been found to be the preferred target range. If the temperature increases, then impurities were found to be generated. The addition of the acid mixture took about 0.5 hr. The mixture was stirred for 20 minutes and then quenched into 0.35 L of cold saturated sodium bicarbonate solution. The pH was corrected to 7 by the addition of solid sodium bicarbonate to the aqueous later. The organic phase was separated and the aqueous layer extracted with 0.4 L of dichloromethane. The organic phases were combined and washed with 0.6 L of saturated sodium chloride solution. The organic phase containing 2′,3′-isopropylidene-N6-cyclopentyladenosine-5′-nitrate was then separated for use in Step 4 below.
Step 4: Because of its lability the protected 2′,3′-isopropylidene-N6-cyclopentyladenosine-5′-nitrate was hydrolyzed directly without purification. The solution from Step 3 was evaporated at 20 degrees C. under vacuum to an oil. The oil was cooled to less than 2 degrees C. 1.95 L of trifluoroacetic acid:water (3:1) solution was added. The reaction mixture was stirred for 0.5 hours and allowed to warm to room temperature while being stirred. After that, the sodium bicarbonate solution was prepared and cooled to less than 10 degrees C. The sodium bicarbonate solution was added to the reaction mixture to quench the reaction. The ethyl acetate was added to the reaction vessel and the pH was adjusted and the organic layer was worked up and dried with sodium sulfate. The resulting product solution was then dried several times with magnesium sulfate and the material stripper to form crude Compound A.
The crude compound A was then recrystallized from ethanol. The crude compound A material was dissolved in ethanol then concentrated to half volume to crystallize for 36 hours. After that the resulting product was isolated by filtration to provide Compound A. 1H-NMR (DMSO-d6): δ 1.49-1.58 (m, 4H), 1.66-1.72 (m, 2H), 1.89-1.94 (m, 2H), 4.12-4.17 (m, 1H), 4.28-4.33 (m, 1H), 4.48 (bs, 1H), 4.65-4.87 (m, 3H), 5.5 (d, J=5.1 Hz, 1H), 5.63 (d, J=5.7 Hz, 1H), 5.91 (d, J=5.1 Hz, 1H), 7.75 (d, J=7.5 Hz, 1H), 8.17 (bs, 1H), 8.30 (s, 1H); MS (ES+): m/z 381.35 (M+1); Anal. Calculated for C15H20N6O6: C, 47.37; H, 5.30; N, 22.10. Found: C, 47.49; H, 5.12; N, 21.96.







Preparation of Polymorphs A1 and A2





During the preparation of ophthalmic solutions of Compound A, variability was seen in particle growth size and stability. Because of the variability, efforts have been made to establish if one or more polymorphs could be isolated and purified in order to overcome the variability in particle size growth and stability.
Crystallization Study:
The Compound A material used for crystallization experiments was taken from a CMC batch prepared substantially as described in steps 1 to 4 above, which was subsequently found to comprise a mixture of approximately 67 percent of form A1 and approximately 33 percent of Form A2.
Form A1
Several slow evaporations crystallisations as detailed in Table 1 below gave crystals using solvents ethyl acetate, isopropyl, acetate, MEK and 2-methoxyethanol that were used for establishing the crystal and molecular structure of Form A1 as shown in FIGS. 1 and 2. It has also been found that a second recrystallization from ethanol of Compound A obtained in step 4 above also yields a substantially pure form of polymorph A1. It is critical in the further recrystallization from ethanol that no moisture from the atmosphere be allowed to condense on the wet cake of compound A. This is because impurities have the potential to form in the presence of water. The preferred recrystallisation process from ethanol then dries the recrystallized compound in a freeze dryer at room temperature.







TABLE 1







Results of the slow evaporation crystallization experiments.










Solvents
μl of solvents
Temperature
Crystals





1,4-Dioxane
400
RT
Form A1


MEK*
400
RT
Too small


Trifluoroethanol
400
RT
Too small


Ethyl Acetate*
400
RT
Form A1


Isopropyl acetate*
400
RT
Form A1


1,2-Dimethoxyethane
400
RT
Glass


2-Methoxyethanol
400
RT
Form A1


3-Methyl-2-butanone*
400
RT
Form A1


DMF
400
RT
Glass


Iso-propanol*
400
RT
Too small


Ethanol/Water (80:20)*
400
RT
Too small


Ethanol/Water (90:10)*
400
RT
Too small





*To dissolve the material, the mixture was warmed up to 60° C. and kept at this temperature for approximately 30 min. Following, it was left for crystallization at room temperature (RT).


MEK: Methylethyl ketone.


DMF: Dimethylformamide.






Approximately 3-8 mg of Compound A was placed into 8 ml vials to which 400 μL of solvent as detailed in Table 1 was added. The experiments were carried out at room temperature. Each 8 ml vial was placed in a 20 ml vial that was then closed and a small hole was pierced in the cap of the 20 ml vials. The vials were left at room temperature. A single colorless crystal (plate shaped) of approximate size 0.35×0.25×0.05 mm was directly collected from the ethyl acetate solution and mounted on a goniometer. The measurements were performed at room temperature (296K). The final crystallographic data are as shown in Table 2 below:







TABLE 2





Crystal data and Structure refinement for Compound A - Form A1


















Identification
Form A1



Empirical Formula
C15H20N606;



Formula Weight
Mr = 380.37;



Crystal System
Monoclinic crystal system;



Space Group
P21 space group;



Unit Cell Dimensions
a = 5.546(2) Å;




b = 7.107(2) Å;




c = 21.929(9) Å;




V = 858.8(5) Å3



[degrees]
96.501(8)



Z
2.



T[K]
296(2)



Å
0.71073



Dc [g/cm3]
1.471



[mm−1]
0.115



F(000)
400



Crystal size [mm3]
0.35 × 0.25 × 0.05



Range of data collection [degrees]
3-27.4



Reflections collected
5868



Independent reflections
3315 [Rint = 0.0268]



Completeness to = 27.4 [%]
97.8



Max. and min. transmission
0.9942 and 0.9606



Data/restraints/parameters
3315/1/289



Goodness-of-fit on F2
1.063



Final R indices[l > 2(l)]
R1 = 0.0418, wR2 = 0.0970



R indices (all data)
R1 = 0.0556, wR2 = 0.1050



Absolute structure parameter
−0.1(12)



Extinction coefficient
0.081(8)










The single crystal measurements were performed on Nonius Kappa-CCD diffractometer equipped with Oxford Cryostream Liquid Nitrogen Cooler using MO K radiation. The data for form A1 was collected up to theta=27.5° at 296K yielding 5868 reflections. Data reduction was performed using HKL Scalepack (Otwinowski & Minor 1997) and cell parameters were obtained using Denzo and Scalepak (Otwinowski & Minor 1997) from 2569 within theta range 1 to 27.5°. The structure was solved using direct methods by SHELXZ-97 (Sheldrick, G. M. 1997a).
In addition to the single x-ray crystallography data, powder diffraction data was also collected on a D8 Advance diffractometer using CuKα1 radiation (1.54016 Å) with germanium monochromator at Room Temperature. The data were collected from 2.5 to 32.5° theta with 0.016° theta steps on solid state LynxEye detector. The sample was measured in an 8 mm long capillary with 0.5 mm diameter.
Crystalline anhydrous polymorph form A1 is preferably characterized by a PXRD spectra having peaks at about 17.5, 20.5, 21.2, 22.7, 24.8, 33.2 and 42.1+0.2 degrees 2 theta.
In Table 3 the intensity, 2 theta and D spacing are listed together with the HKL indices. Because intensity as well as 2 theta values are dependent on the radiation used, therefore the D spacing was implemented. The radiation used was CuKα1.2







TABLE 3







HKL, 2 theta, D spacing and intensity from the powder diffraction of


Form A1 (P21)














h
k
l
D spacing
2θ
Intensity


















0
0
1
21.757
4.058
3.070(29)



0
0
2
10.878
8.121
1.910(36)



0
0
3
7.252
12.194
0.623(59)



0
1
1
6.745
13.115
0.025(65)



0
1
2
5.943
14.895
2.323(93)



−1
0
1
5.498
16.109
 3.19(30)



1
0
0
5.480
16.162
 6.84(33)



0
0
4
5.439
16.283
 0.91(15)



−1
0
2
5.192
17.064
 1.06(15)



1
0
1
5.147
17.214
 4.07(16)



0
1
3
5.072
17.472
11.87(17)



−1
0
3
4.697
18.878
 0.92(18)



1
0
2
4.642
19.104
16.40(23)



0
0
5
4.351
20.393
 0.5(17)



1
−1
−1
4.346
20.420
 20.7(26)



1
1
0
4.337
20.462
 19.5(15)



0
1
4
4.317
20.559
10.14(40)



1
−1
−2
4.190
21.187
42.01(46)



1
1
1
4.166
21.309
 7.14(92)



−1
0
4
4.160
21.342
 1.29(81)



1
0
3
4.106
21.624
 1.29(24)



1
−1
−3
3.916
22.686
77.44(52)



1
1
2
3.884
22.876
12.02(34)



0
1
5
3.709
23.971
 2.41(28)



−1
0
5
3.664
24.270
 0.03(28)



0
0
6
3.626
24.530
 1.18(60)



1
0
4
3.617
24.590
 5.78(63)



1
−1
−4
3.589
24.791
22.15(38)



1
1
3
3.554
25.035
 5.20(97)



0
2
0
3.547
25.082
14.93(93)



0
2
1
3.501
25.419
 9.96(33)



0
2
2
3.373
26.405
 0.01(32)



1
−1
−5
3.256
27.371
 1.19(38)



−1
0
6
3.238
27.525
 0.76(70)



0
1
6
3.229
27.604
 2.8(13)



1
1
4
3.223
27.658
12.60(99)



1
0
5
3.198
27.873
 0.26(46)



0
2
3
3.187
27.977
 0.30(44)



0
0
7
3.108
28.699
 0.65(36)



1
−2
−1
2.981
29.953
 14.3(20)



1
2
0
2.978
29.982
 0.2(25)



0
2
4
2.971
30.050
 5.21(90)



1
−1
−6
2.946
30.318
 7.63(44)



1
−2
−2
2.929
30.494
 1.64(66)



1
2
1
2.921
30.581
 0.0(11)



1
1
5
2.916
30.638
 2.36(86)



−1
0
7
2.881
31.021
 5.24(41)



1
0
6
2.848
31.390
 2.6(62)



0
1
7
2.847
31.397
 0.1(62)



1
−2
−3
2.831
31.580
11.04(53)



1
2
2
2.819
31.720
 3.23(48)



−2
0
1
2.766
32.335
 1.54(44)



0
2
5
2.750
32.539
 4.6(62)



−2
0
2
2.749
32.548
 1.3(64)



2
0
0
2.740
32.657
 1.45(63)



0
0
8
2.720
32.908
 0.37(43)



1
−2
−4
2.699
33.163
18.54(59)



−2
0
3
2.689
33.286
 0.53(97)



1
2
3
2.684
33.350
 0.65(92)



2
0
1
2.673
33.500
 2.6(11)



1
−1
−7
2.669
33.550
 0.12(97)



1
1
6
2.643
33.894
 0.46(44)



−2
0
4
2.596
34.521
 1.31(47)



−1
0
8
2.583
34.701
 0.04(83)



2
−1
−1
2.577
34.778
 1.6(15)



2
0
2
2.574
34.832
 0.3(12)



2
−1
−2
2.563
34.978
 1.06(92)



2
1
0
2.556
35.081
   0(15)



1
0
7
2.556
35.086
   3(15)



1
−2
−5
2.549
35.182
 9.2(11)



0
1
8
2.539
35.316
 2.7(16)



0
2
6
2.536
35.369
 2.3(26)



1
2
4
2.533
35.412
 4.6(16)



2
−1
−3
2.515
35.673
 7.10(49)



2
1
1
2.501
35.874
 0.56(48)



−2
0
5
2.479
36.208
 0.01(47)



2
1
5
2.089
43.279
 5.9(61)



1
−2
−8
2.088
43.295
 0.3(65)



2
2
2
2.083
43.403
 2.0(33)



0
1
10
2.080
43.471
   2(110)



−2
0
8
2.080
43.474
   1(120)



0
3
5
2.078
43.519
 0.7(59)



1
2
7
2.074
43.615
 9.8(13)



1
−3
−4
2.056
44.008
 9.4(15)



2
0
6
2.053
44.070
 0.0(17)



1
3
3
2.049
44.156
 2.0(11)



1
−1
−10
2.036
44.452
 5.63(92)



2
−2
−5
2.032
44.555
 7.3(10)



1
1
9
2.019
44.850
 2.3(18)



2
2
3
2.018
44.889
 0.0(21)



0
2
9
1.998
45.362
   0(670)



2
−1
−8
1.996
45.403
   70(970)










Form A2

None of the crystallization trials or techniques attempted, including (i) slow evaporation of solvent, (ii) vapor diffusion of non polar solvent into liquid solution of Compound A and (iii) polar solvent and temperature controlled crystallization with slow cooling rate; yielded suitable crystals of Form A2 for single crystal analysis. In some experiments, such as, for example, in the temperature controlled crystallizations using various mixtures of ethanol/water, very thin needles were obtained. In most of the cases the crystals seemed to be twinned crystals, however none of these crystals gave enough reflections to obtain proper cell parameters. These crystals were however used to attempt X-ray powder diffraction. Therefore the X-ray Powder Diffraction Pattern (XRPD) was obtained and attempts were then made for solving the structure of the Form A2 from the powder data. The first step was to obtain the proper unit cell. After several trials, two possible cell settings were obtained. Both were orthorhombic although with different Bravais face centering. One of these cells was a face centred cell C, while the other was primitive P. Based on the fact that the cell C could be transformed into a smaller one, namely P, the latter was refined and attempts to solve the structure with this configuration setting were made. Also, with the P cell the asymmetric unit was reduced to 1 molecule with C it concerned 2 symmetry independent molecules. For the cell refinement the Pawley fit was used. A Pawley fit based on the high resolution X-ray diffraction pattern was used to check the purity of the sample. The main purpose of the Pawley fit is to refine cell parameters from the complete pattern. In the Pawley method, profiles are analytical, their width is constrained to follow a Caglioti law with the three refinable parameters U, V, W as defined in most of the Rietveld-derived software. The software used for calculation in this project was Topas with following criteria of fit:
Yo,m and Yc,m are the observed and calculated data, respectively at data point m.
M the number of data points,
P the number of parameters,
Wm the weighting given to data point m which for counting statistics is given by wm=1/σ (Yo,m)2 where σ (Yo,m) is the error in Yo,m



  
    
      
        R
        exp
      
      =
      
        
          
            M
            -
            P
          
          
            ∑
            
              
                w
                m
              
              ⁢
              
                Y
                
                  c
                  ,
                  m
                
                2
              
            
          
        
      
    
    ;
    
      
        R
        wp
      
      =
      
        
          
            ∑
            
              
                
                  w
                  m
                
                ⁡
                
                  (
                  
                    
                      Y
                      
                        o
                        ,
                        m
                      
                    
                    -
                    
                      Y
                      
                        c
                        ,
                        m
                      
                    
                  
                  )
                
              
              2
            
          
          
            ∑
            
              
                w
                m
              
              ⁢
              
                Y
                
                  c
                  ,
                  m
                
                2
              
            
          
        
      
    
    ;
  




  
    
      R
      p
    
    =
    
      
        
          ∑
          
            
            
              
                Y
                
                  o
                  ,
                  m
                
              
              -
              
                Y
                
                  c
                  ,
                  m
                
              
            
            
          
        
        
          ∑
          
            Y
            
              c
              ,
              m
            
          
        
      
    
  




  
    GOF
    =
    
      
        chi
        2
      
      =
      
        
          
            R
            wp
          
          
            R
            exp
          
        
        =
        
          
            
              ∑
              
                
                  
                    w
                    m
                  
                  ⁡
                  
                    (
                    
                      
                        Y
                        
                          o
                          ,
                          m
                        
                      
                      -
                      
                        Y
                        
                          c
                          ,
                          m
                        
                      
                    
                    )
                  
                
                2
              
            
            
              M
              -
              P
            
          
        
      
    
  










TABLE 4





Parameters of the Pawley fit for Compound A - Form A2


















Identification
Form A2



T[K]
293(2)



Å
1.54056



Crystal System
Orthorhombic crystal system;



Space Group
P212121 space group;



Unit Cell Dimensions
a = 5.51796(17) Å;




b = 7.14615(29) Å;




c = 42.9738(29) Å;




V = 1694.55(14) Å3



Capillary size
0.5 × 0.8



Range for data collection
2-22.5



Rexp
1.52



Rwp
2.64



Rp
1.91



RBragg
7.8



GOF
1.74










For the structure solution, the Topas 3.0 software was employed (Bruker-AXS, 2005) using simulated annealing method. The model was built on the Z-matrix and several torsion angles were set as free variables. The obtained model was not refined except for the unit cell. The H-atoms were included based on geometry and H-Bond scheme. FIG. 3 shows the molecular structure of Form 2 of Compound A and FIG. 4 shows the crystal packing and the H-bond scheme.
XRPD patterns were obtained using a high-throughput XRPD set-up. The plates were mounted on a Bruker GADDS diffractometer equipped with a Hi-Star area detector. The XRPD platform was calibrated using Silver Behenate for the long d-spacings and Corundum for the short d-spacings.
Data collection was carried out at room temperature using monochromatic CuKα radiation in the 2-theta region between 1.5 degrees and 41.5 degrees, which is the most distinctive part of the XRPD pattern between the polymorph forms. The diffraction pattern of each well was collected in 2 theta ranges (1.5 degrees≦2 theta≦21.5 degrees for the first frame, and 19.5 degrees≦2 theta≦41.5 degrees for the second) with an exposure time of 30 seconds for each frame. No background subtraction or curve smoothing was applied to the XRPD patterns. The carrier material used during XRPD analysis was transparent to X-rays and contributed only slightly to the background.
Crystalline anhydrous polymorph form A2 is preferably characterized by PXRD spectra having peaks at about 16.9, 18.1, 19.1, 20.8, 21.3, 22.0, 22.8, 23.8, 24.9, 25.0, 29.1, 29.8, 34.2 and 35.8±0.2 degrees 2 theta.
In Table 5 the intensity, 2 theta and D spacing are listed together with the HKL indices. Because intensity as well as 2 theta values are dependent on the radiation used, therefore the D spacing was implemented. The radiation used was CuKα1.2







TABLE 5







HKL, 2 theta, D spacing and intensity from the powder diffraction of


Form A2 (P212121)














h
k
l
D spacing
2θ
Intensity


















0
0
2
21.487
4.109
3.341(28)



0
0
4
10.743
8.223
2.277(38)



0
0
6
7.162
12.348
0.690(57)



0
1
1
7.049
12.547
0.802(58)



0
1
2
6.781
13.045
0.032(57)



0
1
3
6.395
13.837
1.088(68)



0
1
4
5.950
14.877
2.330(82)



0
1
5
5.495
16.117
 2.50(22)



1
0
1
5.473
16.182
16.69(24)



0
0
8
5.372
16.489
 0.31(12)



1
0
2
5.345
16.574
 0.02(12)



1
0
3
5.149
17.207
 0.78(11)



0
1
6
5.059
17.517
 9.55(14)



1
0
4
4.908
18.058
15.49(17)



0
1
7
4.657
19.043
 2.50(33)



1
0
5
4.643
19.098
18.42(35)



1
0
6
4.371
20.300
 0.0(12)



1
1
0
4.367
20.317
 14.0(13)



1
1
1
4.345
20.423
21.46(33)



0
0
10
4.297
20.652
 4.4(20)



0
1
8
4.294
20.669
 14.2(23)



1
1
2
4.280
20.737
34.73(53)



1
1
3
4.178
21.251
62.18(36)



1
0
7
4.104
21.637
 3.26(21)



1
1
4
4.046
21.951
64.81(39)



0
1
9
3.970
22.375
 3.18(21)



1
1
5
3.894
22.821
67.15(41)



1
0
8
3.849
23.089
 0.02(22)



1
1
6
3.729
23.844
23.77(31)



0
1
10
3.683
24.147
 1.11(24)



1
0
9
3.611
24.636
 5.44(27)



0
0
12
3.581
24.843
 1.32(64)



0
2
0
3.573
24.900
 0.0(10)



0
2
1
3.561
24.987
 16.0(37)



1
1
7
3.559
25.001
 57.1(34)



0
2
2
3.525
25.247
 7.78(28)



0
2
3
3.467
25.675
 0.11(25)



0
1
11
3.428
25.972
 0.02(26)



0
2
4
3.390
26.264
    0(1200)



1
0
10
3.390
26.264
    0(1200)



1
1
8
3.389
26.278
  13(11)



0
2
5
3.299
27.003
 0.24(27)



1
1
9
3.223
27.658
 6.71(31)



0
1
12
3.202
27.843
 4.61(77)



0
2
6
3.197
27.882
 0.02(89)



1
0
11
3.188
27.961
 0.02(42)



0
2
7
3.088
28.889
 3.68(33)



0
0
14
3.070
29.067
 0.02(57)



1
1
10
3.063
29.129
13.39(58)



1
0
12
3.004
29.716
 0.3(17)



0
1
13
3.000
29.754
 0.3(90)



1
2
0
2.999
29.765
 4.9(81)



1
2
1
2.992
29.839
23.29(88)



0
2
8
2.975
30.012
 0.81(68)



1
2
2
2.970
30.060
 4.79(66)



1
2
3
2.936
30.426
 0.16(34)



1
1
11
2.912
30.680
 1.09(34)



1
2
4
2.889
30.931
 2.18(35)



0
2
9
2.861
31.241
 3.31(36)



1
0
13
2.836
31.524
 2.60(83)



1
2
5
2.832
31.569
13.87(87)



0
1
14
2.820
31.700
 0.94(41)



1
1
12
2.769
32.301
 1.3(12)



1
2
6
2.766
32.335
 12.9(14)



2
0
0
2.759
32.425
 2.17(88)



2
0
1
2.753
32.493
 3.93(89)



0
2
10
2.747
32.564
 2.65(63)



2
0
2
2.737
32.698
 1.03(41)



2
0
3
2.709
33.037
 0.47(39)



1
2
7
2.695
33.219
13.32(50)



0
0
16
2.686
33.333
 0.44(92)



1
0
14
2.682
33.376
 0.02(88)



2
0
4
2.672
33.507
 1.62(43)



0
1
15
2.659
33.677
 0.31(39)



0
2
11
2.637
33.974
 0.0(33)



1
1
13
2.636
33.985
 1.5(34)



2
0
5
2.627
34.103
 3.97(60)



1
2
8
2.619
34.214
14.87(53)



2
0
6
2.575
34.818
 0.3(46)



2
1
0
2.574
34.829
 2.2(52)



2
1
1
2.569
34.893
 4.43(92)



2
1
2
2.556
35.086
 4.65(43)



1
0
15
2.543
35.270
 0.0(11)



1
2
9
2.540
35.312
 11.6(13)



2
1
3
2.533
35.405
 0.15(98)



0
2
12
2.529
35.461
 5.87(80)



2
0
7
2.517
35.648
 0.0(15)



0
1
16
2.514
35.683
 0.0(25)



1
1
14
2.511
35.724
 2.5(15)



2
1
4
2.503
35.847
15.03(56)



2
1
5
2.466
36.409
 3.57(54)



1
2
10
2.459
36.504
 1.95(65)



2
0
8
2.454
36.585
 0.02(56)



0
2
13
2.427
37.018
 2.26(64)



2
1
6
2.422
37.086
 1.62(68)



1
0
16
2.415
37.201
 1.13(49)



1
1
15
2.396
37.514
 2.28(58)



2
0
9
2.389
37.623
 4.3(25)



0
0
18
2.387
37.646
 0.0(30)



0
1
17
2.383
37.716
 0.6(17)



1
2
11
2.379
37.785
 8.3(82)



0
3
1
2.378
37.795
 2.2(80)



2
1
7
2.374
37.873
 2.95(98)



0
3
2
2.368
37.974
 1.72(55)



0
3
3
2.350
38.273
 0.02(46)



0
2
14
2.328
38.639
 6.6(11)



0
3
4
2.326
38.687
 2.9(18)



2
0
10
2.322
38.754
 0.9(66)



2
1
8
2.321
33.764
 1.8(59)



1
2
12
2.299
39.146
 1.3(32)



1
0
17
2.298
39.167
 0.0(43)



0
3
5
2.296
39.214
 11.8(17)



1
1
16
2.288
39.351
 2.64(54)



2
1
9
2.266
39.753
 5.2(23)



0
1
18
2.264
39.775
 0.0(26)



0
3
6
2.260
39.850
 1.52(90)



2
0
11
2.254
39.973
 4.10(57)



0
2
15
2.235
40.318
 1.37(55)



1
2
13
2.221
40.582
 0.5(43)



0
3
7
2.221
40.591
 4.1(43)



2
1
10
2.208
40.835
 0.02(60)



1
0
18
2.191
41.165
 0.0(15)



1
1
17
2.188
41.230
   3(14)



1
3
0
2.187
41.246
   1(25)



2
0
12
2.186
41.274
   2(24)



1
3
1
2.184
41.302
   6(49)



2
2
0
2.184
41.310
   0(40)



2
2
1
2.181
41.366
 8.8(38)



0
3
8
2.178
41.433
 2.1(33)



1
3
2
2.176
41.469
 11.6(29)



2
2
2
2.173
41.533
 2.8(12)



1
3
3
2.162
41.747
 11.1(12)



2
2
3
2.159
41.810
 7.1(18)



0
1
19
2.156
41.860
 2.7(15)



2
1
11
2.149
42.003
 5.0(99)



0
0
20
2.149
42.016
   0(14)



0
2
16
2.147
42.052
 0.9(81)



1
2
14
2.145
42.087
 2.4(56)



1
3
4
2.143
42.132
 3.6(29)



2
2
4
2.140
42.195
 6.3(12)



0
3
9
2.132
42.370
 5.30(72)



1
3
5
2.119
42.624
 2.3(32)



2
0
13
2.118
42.651
 0.2(49)



2
2
5
2.116
42.686
 6.8(25)



1
1
18
2.095
43.148
 1.4(22)



1
0
19
2.093
43.194
 0.2(81)



1
3
6
2.092
43.219
   3(11)



2
1
12
2.090
43.254
 6.0(89)



2
2
6
2.089
43.280
 2.5(44)



0
3
10
2.083
43.398
 0.10(84)



1
2
15
2.072
43.657
 6.91(88)



0
2
17
2.064
43.835
 0.0(11)



1
3
7
2.060
43.913
 5.9(22)



0
1
20
2.058
43.968
   4(18)



2
2
7
2.057
43.974
   0(17)



2
0
14
2.052
44.098
 0.99(86)



0
3
11
2.034
44.512
 1.2(11)



2
1
13
2.031
44.581
 6.0(12)



1
3
8
2.026
44.704
 10.2(16)



2
2
8
2.023
44.764
 8.8(23)



1
1
19
2.008
45.105
   0(38)



1
0
20
2.002
45.252
    0(2700)



1
2
16
2.001
45.286
    0(4300)










Controlling the Formation of the Form of Polymorph

It has been found that the formation of the particular polymorphic form can be controlled. As described above the Form A1 can be obtained predominantly via recrystallization from ethanol or under slow evaporation conditions. It has also been established that ripening or curing of Compound A particles suspended in an aqueous suspension formulated for ocular delivery at 40 degrees C. for a relatively short period of time formed polymorph form A2 from polymorph form A1. The aqueous suspension samples were kept at 40 degrees C. for up to 108 hours and monitored by particle size measurement, XRPD and microphotography. Particle size measurements showed that average sizes increased significantly over 15 hours. Thereafter, sizes remained effectively constant to 108 hours. XRPD analyses indicated a change in polymorph content from about 74% A1 to 26% A2 at time zero to 0% A1 to 100% A2 at 108 hours at 40 degrees C. FIG. 7 shows the conversion of form A1 to A2 over time. Also, habit changes coupled with A2 growth were reflected in the XRPD patterns and could be monitored by a difference in selected peak intensities from planes within the crystal lattice perpendicular to the c axis that change in intensity as the habit of the crystal changes. The intensity differences changed up to 9 hours and remained constant thereafter indicating that the habit changes were completed during this time. Microphotographs showed blade or plate-like crystal habits of particles in suspension.
When the aqueous suspension ocular formulation containing Compound A in the A1 polymorph is stored at 2-8° C., a temperature required to limit decomposition of Compound A over long term storage, the habit of the suspended particles changes slowly over a period of 6 to 12 months. During this time the small irregular particles of suspended drug change to rod-like habits, with many particles having a length along the longest dimension over 100 microns. These changes make it much more difficult to resuspend Compound A particles by sonication and shaking in order to form a homogeneous suspension for dosing.
The conversion of the A1 form to the A2 form has been found to limit any further changes to particle habit, size or polymorph content when the aqueous suspension, which is suitable for ocular delivery of the drug, is stored over a 6 month period at either 5° C. or 25° C. Also, the cured aqueous suspension is more easily resuspended by shaking, a favourable characteristic for suspension formulations for ocular drug delivery.
The particle size analyses were performed on a Cilas 1180 Particle Size Analyzer. The parameters used were liquid mode, sample refractive index=1.62 (determined using Cargille immersion oils), liquid refractive index=1.333 (value for water), 30 second measurement, 180 rpm stifling, 120 rpm pump circulation, no sonication, 5 repeat measurements.
Formulation Example
A batch of sterile material of Compound A was prepared as described above under the “Synthesis of Compound A”. The resulting Compound A material was then sterilized with gamma irradiation at up to 40 kGray and then formulated into the following aqueous formulations:
Aqueous Formulation














Ingredient
%, W/V









Compound A
0.152-0.76



Sodium CMC
0.7



Benzalkonium Chloride
0.01



Polysorbate 80
0.3



Citric Acid Monohydrate
0.152 (7 mM)



NaOH/HCl
pH 5.1 ± 0.1



NaCl
q.s. to 270-330 mOsm



Purified Water
q.s. to 100.00










Various concentrations of Compound A formulation lots were prepared from 0.152, 0.30, 0.61, 0.91, 2.42, 0.46, 0.76%, W/V to provide for the ability to deliver different levels of Compound A per drop of formulation. For example one drop of the 0.152%, W/V of compound A would deliver 50 mcg per drop, 0.30%, W/V would deliver 100 mcg per drop, right through to 0.76% W/V delivering 250 mcg per drop. The formulation lots were then heated to undergo the curing step and convert the A1 polymorph form of Compound A to the A2 polymorph form of Compound A. The curing step was undertaken by placing the formulation lots at 40 degrees C. for 48 hours and then reverting the formulations lots to the desired longer term storage conditions for stability studies.
Two of the formulation lots, namely 0.46% W/V of Compound A and a 0.76% W/V were studied for long term stability and particle size growth at 5 degrees Celsius and 25 degrees Celsius for 6 months. Two of the formulation lots, namely 0.46% W/V of Compound A and a 0.76% W/V were studied for long term stability and particle size growth at 5 degrees Celsius for 18 months. The results are tabulated below in Table 6.











TABLE 6









Particle Size



Time


Distribution


Formulation
(months)
Impurities
pH
(microns)



















0.46% at 5° C.
0
1%
5.1
X10 = 1.746






X50 = 6.992






X90 = 14.087


0.46% at 5° C.
1
1%
5.0
X10 = 0.907






X50 = 6.285






X90 = 13.485


0.46% at 5° C.
3
1%
5.0
X10 = 1.792






X50 = 7.082






X90 = 14.356


0.46% at 5° C.
6
1%
5.1
X10 = 1.777






X50 = 6.939






X90 = 13.698


0.46% at 5° C.
12
1%
5.1
X10 = 1.398






X50 = 6.679






X90 = 13.396


0.46% at 5° C.
18
1%
5.1
X10 = 1.666






X50 = 6.882






X90 = 13.074


0.46% at 25° C.
0
1%
5.1
X10 = 1.746






X50 = 6.416






X90 = 13.698


0.46% at
1
1%
5.0
X10 = 1.036


25° C./60% RH



X50 = 6.416






X90 = 13.698


0.46% at
3
3%
5.1
X10 = 1.656


25° C./60% RH



X50 = 6.705






X90 = 12.805


0.46% at
6
4%
5.0
X10 = 1.809


25° C./60% RH



X50 = 6.741






X90 = 12.380


0.76% at 5° C.
0
1%
5.1
X10 = 1.524






X50 = 6.773






X90 = 12.778


0.76% at 5° C.
1
1%
5.1
X10 = 1.115






X50 = 6.456






X90 = 12.944


0.76% at 5° C.
3
1%
5.1
X10 = 1.455






X50 = 6.745






X90 = 13.104


0.76% at 5° C.
6
1%
5.1
X10 = 1.541






X50 = 6.638






X90 = 11.833


0.76% at 5° C.
12
1%
5.1
X10 = 1.407






X50 = 6.635






X90 = 12.314


0.76% at 5° C.
18
1%
5.1
X10 = 1.611






X50 = 6.840






X90 = 12.672


0.76% at
0
1%
5.1
X10 = 1.524


25° C./60% RH



X50 = 6.773






X90 = 12.778


0.76% at
1
1%
5.1
X10 = 1.056


25° C./60% RH



X50 = 6.107






X90 = 11.551


0.76% at
3
2%
5.1
X10 = 1.446


25° C./60% RH



X50 = 6.691






X90 = 12.724


0.76% at
6
3%
5.1
X10 = 1.619


25° C./60% RH



X50 = 6.292






X90 = 10.240









It can be seen from the results in Table 6 that the particle size distributions of the two formulation lots are stable over the time under the conditions tested. The results also show that the levels of impurities and pH remain stable for the formulations at 5 degrees Celsius over 18 months, while there is a slow increase in the impurities for the formulations held at 25 degrees Celsius over 6 months.
The present invention and its embodiments have been described in detail. However, the scope of the present invention is not intended to be limited to the particular embodiments of any process, manufacture, composition of matter, compounds, means, methods, and/or steps described in the specification. Various modifications, substitutions, and variations can be made to the disclosed material without departing from the spirit and/or essential characteristics of the present invention. Accordingly, one of ordinary skill in the art will readily appreciate from the disclosure that later modifications, substitutions, and/or variations performing substantially the same function or achieving substantially the same result as embodiments described herein can be utilized according to such related embodiments of the present invention. Thus, the following claims are intended to encompass within their scope modifications, substitutions, and variations to processes, manufactures, compositions of matter, compounds, means, methods, and/or steps disclosed herein.

## Claims

1. A crystalline anhydrous polymorph A1 of Compound A, [2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)] methyl nitrate:



having the following crystal data,
C15H20N6O6;
Mr=380.37;
Monoclinic crystal system;
P21 space group;
a=5.546(2) Å;
b=7.107(2) Å;
c=21.929(9) Å;
V=858.8(5) Å3, and
Z=2;
wherein the crystalline polymorph A1 is at least about 75% free of other solid forms of Compound A.

2. The crystalline anhydrous polymorph A1 of claim 1 having substantially equivalent peaks at a reflection angle 2-theta as shown in Table 3.

3. The crystalline anhydrous polymorph of claim 1 that is at least about 80% free of other solid forms of Compound A.

4. The crystalline anhydrous polymorph of claim 1 that is at least about 90% free of other solid forms of Compound A.

5. The crystalline anhydrous polymorph of claim 1 that is at least about 95% free of other solid forms of Compound A.

6. The crystalline anhydrous polymorph of claim 1 that is at least about 99% free of other solid forms of Compound A.

7. The crystalline anhydrous polymorph of claim 1 that is 100% free of other solid forms of Compound A.

8. A method of obtaining the crystalline anhydrous polymorph of claim 1, the method comprising the steps of preparing compound A as an active pharmaceutical ingredient (API) by recrystallizing at least twice from ethanol.

9. A method of obtaining the crystalline anhydrous polymorph of claim 1, the method comprising the step of recrystallizing Compound A from isopropanol, ethyl acetate, or isopropyl acetate.

10. A method of obtaining the crystalline anhydrous polymorph of claim 1, the method comprising the step of recrystallizing Compound A from 1,4 dioxane, 2-methoxy ethanol, 3-methyl-2-butanone, methylethyl ketone, or 1,2-dimethoxyethane.

11. A pharmaceutical composition comprising the crystalline anhydrous polymorph A1 of claim 1 and one or more pharmaceutically acceptable ingredients selected from the group consisting of carriers, excipients, diluents, additives, fillers, surfactants, binders, antimicrobial preservatives, viscosity enhancing agents, and buffers.

12. The pharmaceutical composition of claim 11 that is formulated for ophthalmic administration.

13. A crystalline anhydrous polymorph A2 of Compound A, [2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)] methyl nitrate:



having the following crystal data,
C15H20N6O6;
Mr=380.37;
Orthorhombic crystal system;
P212121 space group;
a=5.51796(17) Å;
b=7.14615(29) Å;
c=42.9738(29) Å and
V=1694.55(14) Å3;
wherein the crystalline polymorph A2 is at least 75% free of other solid forms of Compound A.

14. The crystalline anhydrous polymorph A2 of claim 13 having substantially equivalent peaks at a reflection angle 2-theta as shown in Table 5; wherein the crystalline polymorph A2 is substantially free of other solid forms of Compound A.

15. The crystalline anhydrous polymorph A2 of claim 13 that is 100% free of other solid forms of compound A.

16. A pharmaceutical composition comprising the crystalline anhydrous polymorph A2 of claim 13 and one or more pharmaceutically acceptable ingredients selected from the group consisting of carriers, excipients, diluents, additives, fillers, surfactants, binders, antimicrobial preservatives, viscosity enhancing agents, and buffers.

17. The pharmaceutical composition of claim 16 that comprises:














Ingredient
%, W/V









Compound A
0.152-0.76



Sodium CMC
0.7



Benzalkonium Chloride
0.01



Polysorbate 80
0.3



Citric Acid Monohydrate
0.152 (7 mM)



NaOH/HCl
pH 5.1 ± 0.1



NaCl
q.s. to 270-330 mOsm; and



Purified Water
q.s. to 100.00.

18. The pharmaceutical composition of claim 16 that is formulated for ophthalmic administration.

19. A method of reducing intraocular pressure in a subject, the method comprising topically administering to an eye of a subject in need thereof a therapeutically effective amount of the pharmaceutical composition of claim 18.

20. A method of obtaining the crystalline anhydrous polymorph A2 of claim 13, the method comprising the steps of suspending Compound A in an aqueous liquid vehicle and heating to about 40 degrees for at least 9 hours.

21. The crystalline anhydrous polymorph of claim 13 that is at least about 80% free of other solid forms of Compound A.

22. The crystalline anhydrous polymorph of claim 13 that is at least about 90% free of other solid forms of Compound A.

23. The crystalline anhydrous polymorph of claim 13 that is at least about 95% free of other solid forms of Compound A.

24. The crystalline anhydrous polymorph of claim 13 that is at least about 99% free of other solid forms of Compound A.

25. A crystalline anhydrous polymorph form A1 of Compound A, [2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)] methyl nitrate:



having PXRD spectra peaks at about 17.5, 20.5, 21.2, 22.7, 24.8, 33.2 and 42.1+0.2 degrees 2 theta, wherein the crystalline polymorph A1 is at least 75% free of other solid forms of Compound A.

26. A crystalline anhydrous polymorph form A2 of Compound A, [2R,3S,4R,5R)-5-(6-(cyclopentylamino)-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)] methyl nitrate,



having PXRD spectra peaks at about 16.9, 18.1, 19.1, 20.8, 21.3, 22.0, 22.8, 23.8, 24.9, 25.0, 29.1, 29.8, 34.2 and 35.8+0.2 degrees 2 theta, wherein the crystalline polymorph A2 is at least 75% free of other solid forms of Compound A.