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                            Funding & Awards
 Laser Microdissection Separation of Pure Spermatozoa Populations from Mixed Cell Samples for Forensic DNA Analysis
Award Information
Awardee
              Rosalind Franklin University of Medicine And Science
Award #
              2004-DN-BX-K215
Funding Category
              Competitive
Location
                    North Chicago, IL
Congressional District
              10
Status
              Closed
Funding First Awarded
              2004
Total funding (to date)
              $449,829
Original Solicitation
              Forensic DNA Research & Development
Description of original award (Fiscal Year 2004, $449,829)
This DNA R&D project focuses on development of a method for simultaneous identification and recovery of sperm in sexual assault evidence mixtures using Laser Microdissection (LMD). LMD can directly dissect and collect pure cell populations for immediate DNA isolation without contamination from intermixed foreign cells. The cells of interest are circumscribed through the computer software image and cut by laser ablation, after which they are collected by gravity into a microcentrifuge tube below the stage for further investigation. LMD can provide a solution to cases where mixed genotypes are unresolved or sample analysis is not pursued due to an insufficient number of target cells.  The goal of this project is to demonstrate the effectiveness of LMD and develop protocols for simultaneous identification and recovery of sperm in cellular mixtures that permits better separation of minute traces of male sexual assailant DNA from victims DNA.

ca/ncf



  
Grant-Funded Publications
        
                      Laser Microdissection Separation of Pure Spermatozoa Populations From Mixed Cell Samples for Forensic DNA Analysis
                  Laser Microdissection Separation of Pure Spermatozoa From Epithelial Cells for Short Tandem Repeat Analysis

  
Date Created: September 20, 2004
    
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