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                            Funding & Awards
 An Investigation of the Effect of DNA Degradation and Inhibition on PCR Amplification of Single Source and Mixed Forensic Samples
Award Information
Awardee
              Florida International University
Award #
              2006-DN-BX-K006
Funding Category
              Competitive
Location
                    Miami, FL
Congressional District
              24
Status
              Closed
Funding First Awarded
              2006
Total funding (to date)
              $347,399
Original Solicitation
              NIJ FY06 Forensic DNA Research and Development Solicitation: Invited Full Applications
Description of original award (Fiscal Year 2006, $347,399)
The goal of this proposal is to develop methods to better understand the effect of inhibition and degradation in the recovery of DNA information from casework samples. A better understanding of inhibition mechanisms could lead to the development of more sensitive and robust crime lab protocols. Various analytical techniques (e.g., capillary electrophoresis, mass spectrometry) will be used to detect specific inhibitors in skeletal samples that have been exposed to various environmental conditions. Controlled laboratory experiments studying the effects and mechanisms of specific inhibitors (e.g., humic acid, indigo, and collagen) on DNA templates will also be performed, followed by studies using samples from adjudicated casework. The principal investigator will collaborate with scientists from the University of Tennessee Anthropology Department (for inhibition studies involving skeletal remains), the Vermont Department of Public Safety Crime Lab (for real-time PCR assays examining degradation), the Broward County Crime Lab (for examining adjudicated casework samples), and NIST (for assistance with primer design).
ca/ncf



  
Grant-Funded Publications
        
                      An Analysis of Single and Multi-Copy Methods for DNA Analysis by Real-Time Polymerase Chain Reaction
                  The Application of Amplicon Length Heterogeneity PCR (LH-PCR) for Monitoring the Dynamics of Soil Microbial Communities Associated With cadaver decomposition
                  A Comparison of the Effects of PCR Inhibition in Quantitative PCR and Forensic STR Analysis
                  An Investigation of the Effect of DNA Degradation and Inhibition on PCR Amplification of Single Source and Mixed Forensic Samples
                  The Application of Magnetic Bead Hybridization for the Recovery and STR Amplification of Degraded and Inhibited Forensic DNA
                  An analysis of single and multi-copy methods for DNA quantitation by real-time polymerase chain reaction

  
Date Created: September 10, 2006
    
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