### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 1 of 13 Data Requirement: EPA DP Barcode D 412314

EPA MRID 491213

- 02

EPA Guideline 850.2100 (OCSPP)

Test material: Tribufos Technical Purity: 98.2 %

Common name: Tribufos

Chemical name: IUPAC: S,S,S

- tributyl phosphorotrithioate

CAS: S,S ,S

- tributyl phosphorotrithioate

CAS No.: 78

- 48

- 8

Primary Reviewer: Rebecca Bryan Signature:

Staff Scientist, CSS

- Dynamac Corporation Date: 10/10 /13

Secondary Reviewer: John Marton, Ph.D. Signature:

Environmental Scientist, CDM Smith Date: 11/14/13

Secondary Reviewer(s): Mary Clock

- Rust Signature:

Biologist, ERB4, EFED, OPP, OCSPP, EPA Date: 1/14 /15

EPA PC Code 074801

Date Evaluation Completed: 01

- 1 4

- 2015

CITATION : Dias, N.A. 20 13

. Tribufos : Acute Oral Toxicity ( LD 50 ) to the Zebra Finch

. Unpublished study

performed by Huntingdon Life Sciences, Ltd., Huntingdon, Cambridgeshire, England . Laboratory Study No.

BDG 0143

. Study sponsored by AMVAC Chemical Corporation, Newport B each , CA

. Study initiated June 18

, 2012 and completed April 15, 2013

.

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 2 of 13 EXECUTIVE SUMMARY :

The acute oral toxicity of t ribufos to 8

- to 10

- month old z ebra finch es ( Taeniopygia guttata ) was assessed over 1 4 days.

Tribufos was adm inistered to the birds by oral gavage at nominal levels of 0 ( vehicle control , corn oil )

, 125

, 250

, 500

, 1000 , and 2000 mg /kg bw

. Treatment

- related mortalit y of 10, 10, 10, 10, and 80% w as observed at 125, 250,

500, 1000, and 2000 mg /kg bw groups, respecti vely

. Signs of toxicity were observed in all treatment groups and

included ruffled feathers, underactivity, incoordination, recumb e ncy, body tremors, panting, salivation and/or emesi s

(regurgitation)

. Regurgitation was observed within one hour of dosing fo r 1, 1, 7, 7, and 6 birds in the 125 , 250, 500,

1000, and 2000 mg/

kg groups, respectively. There were no apparent treatment

- related effects upon body weight or

body weight change at any interval for males or females at any treatment group compared to the c ontrol over the 14

- day study period

.

Because the birds regurgitated after dosing, actual exposure is likely lower than the nominal dose. Therefore, the

LD 50 may be lower than th at ( LD 50 = 1560 mg/ kg bw) calculated by the study authors . Due to the result ing

uncertainty in exposure as well as the study endpoint, th e s e study deficienc ies affect the scientific soundness and

acceptability of this study

. Assuming that each of the birds that regurgitated represents a mortality in addition to

those that actually died, the 14

- day LD 50 is estimated at 39 7 mg/kg bw. Based on this value, tribufos would be

classified as slightly toxic to birds on an acute oral exposure basis. Since regurgitation was observed in each of the

treatment groups a definitive NOAEL was not d etermined, i.e., NOAEL<125 mg/ kg bw. This study is classified as

supplemental due to observed regurgitation.

Results Synopsis

Test Organism Size/Age (Weight range ): Adult, 22 weeks old; 14.3 to 24.0 g

LD 50 : 397 mg/

kg bw 95% C.I.: 268

- 588 mg/ kg bw

Endpoint(s) affected : Mortality and signs of toxicity (including regurgitation)

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 3 of 13 I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The study protocol was based upon procedures outlined in U.S. EPA

Ecological Effects Test Guidelines, OCSPP No. 850.2100

, Avian Acute

Oral Toxicity Test ( 2012)

.

Deviations from O CS PP 850.2100 guidance included:

 Concentrations of test substance in the d osing solutions were not analytically

- confirm ed

.

 O ne or more birds at all treated levels regurgitated test material after o ne hour of dosing

.

These deviations affect the scientific soundness of this study and the study classifica t i on

.

COMPLIANCE: Signed and dated GLP

, Quality Assurance, and Data Confidentiality

statements were provided.

A. MATERIALS:

1. Test mater ial : Tribufos technical

Description: Liquid

Lot No./Batch No. : NX81AX0015

Purity: 98.2 %

Stability of compound

under test conditions: Not reported

.

Storage conditions of

test chemicals: Room temperature desiccated.

Physicochemical properties of Tribufos

.

Parameter Values Comments

Water solubility at 20°C 2.3 mg/L MRID 41618803

Vapor pressure at 20°C 1.7 x 10

- 6 torr MRID 41618803

Structure

K

ow at 20°C 3.31 x 10 5 MRID 41618803

(O ECD recommends water solubility, stability in water and light, pKa, Pow, and vapor pressure of test compound)

2. Test Organism :

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Number 49121302

Page 4 of 13 Species (common and scientific names): Zebra finch ( Taeniopygia guttata )

Age at study initiation: Adult, 8 to 10 months old

Weight at study initiation (range): 12.7 to 25.2 g

Source: Huntingdon Life Sciences stock

B. STUDY DESIGN:

1. Experimental Conditions

a. Range-finding study: The definitive test concentrations were based on a range-finding study using 10

birds dosed at 400, 650, 900, and 1500 mg/ kg . Signs of toxicity were observed in all treatment groups and

included minor clinical signs (not described), transiently wiping its beak, underactive, shaking its head,

ruffled feathers, body tremors, panting, and/or recumbency

.

b. Definitive study:

Table 1: Experimental Parameters

Parameter Details Remarks

Criteria

Acclimation

Period:

Conditions: (same as test or not)

Feeding:

Health: (any mortality observed) 14 days

Same as test

A proprietary seed mixture

and water were

available ad libitum

.

Birds in apparent good he alth. The recommended acclimation period is

a minimum of 15 days. OECD

recommends a minimum of 7 days.

Pen size and construction materials : 75 x 45.5 x 46 cm

Constructed of powder

- coated wire

mesh

. Pen size and construction should

conform to goo d husbandry practices

and should not create crowding stress.

OECD recommends that pens be

suitable for the captive rearing of that

species.

Test duration : 1 4 days

Recommended test duration is one day

for dosing and at least 14 days

observation.

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 5 of 13 Parameter Details Remarks

Criteria

Dos e preparation [Indicate method

of confirmation of dose] The appropriate amounts of test

substance were dissolved/dispersed

in corn oil

. Confirmation of dosing was not

assessed.

Mode of dose administration : Gavage at 5 mL/kg dose volume

.

Gavage or gelatin capsule is

recommended

Dose levels

N ominal:

M easured: 0 ( vehicle control), 125

, 250

, 500

, 1000 , and 2000 mg/

kg bw

Not determined Dose levels should be a minimum of 5

treatment levels unless LD 50 is

demonstrated to be greater than 2000

mg/ kg

Solvent/vehicle, if used

Type :

Amount /bw: C orn oil

Not reported The test material should be administered

without a vehicle if possible. Maximum

vehicle should not exceed 0.1 to 1.0% of

body weight.

Number of birds per group/treatment

N egative control:

S olvent/vehicle control:

T reated: N/A

10 (5 per sex)

10 (5 per sex) Recommended number of birds in a

treatment group is 10 and 10 birds for

each control and vehicle group.

No. of feed withholding days before

dosing : At least 3 to 4 hours The feed was withheld for 3

- 4 hours

instead of the recommended 15 hours.

Food should be withheld for at least 15

hours prior to dosing.

Test conditions

Temperature:

Relative humidity:

Photoperiod: 16

- 22 °C

38

- 58 %

10 hours light/ 14 hours dark The recommended photoperiod is 10

hours of light and 14 hours of dark.

Reference chemical, if used

Name :

C oncentrations tested: None tested

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 6 of 13 2. Observations:

Table 2: Observations

Criteria Details Remarks

Criteria

Parameters measured

(mortali ty/individual body weight at

test initiation and termination/ mean

feed consumption/ others) - Mortality

- Clinical signs of toxicity

- Body weight

- Food consumption Body weight should be measured at test

initiation, on day 14 and at the end of

the test if the test is extended beyond 14

days. Mortality should not be more than

10% in controls.

Feed consumption should be measured

as average daily food consumption.

Indicate if the test material was

regurgitated Birds were observed regurgitating

within one hour of dosing in all

treatment groups. Regurgitat ion

(emesis) was observed for 1, 1, 7, 7,

and 6 birds in the 125, 250, 500,

1000, and 2000 mg ai/kg groups,

respectively (12 males and 10

female). Regurgitation is an indication that the

dose was rejected. If this problem

persists, the test should be repeated.

Groups on which necropsies were

performed Necropsies were performed on all

birds that died or were sacrificed

during test. In addition, all control

birds and ten surviving treated birds

fr om the highest dose groups with

survivors were necropsied. Gross necropsies should be performed

with inspections of the GI tract, liver,

kidneys, heart, and spleen.

Observation intervals : Birds were observed for mortality

and clinical signs of toxicit y

continuously for one hour

immediately after dosing and twice

daily thereafter

.

Individual b ody weights were

measured individually on D ays 0 , 7,

and 14.

Food consumption was estimated

for Day

- 1, Days 1

- 7

, and Days 8

-

14.

Were raw data included? Ye s

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 7 of 13 II. REPORTED RESULTS AND DISCUSSION :

A. MORTALITY:

No mortalities were observed in the control. According to the study authors, t reatment

- related mortalities of 10,

10, 10, 10, and 80 % were observed at 125, 250, 500, 1000, and 2000 mg/ kg bw groups , re spectively

. According to the study author, the acute oral LD 50 was calculated as 1560 mg/ kg bw with 95% confidence

interval of 929 to 5660 mg/ kg bw ( Table 3 )

. Assuming that each of the birds that regurgitated represents a

mortality in addition to those t hat actually died, the 14

- day LD 50 is estimated at 397 mg/ kg bw. The NOAEL

was not determined based on mortalities in all treatment groups

.

Table 3: Reported Effect of Tribufos on Mortality in the Zebra F inch

. a

Treatment

( mg/ kg bw ) No. of

Bird s Cumulative Mortality ( birds that regurgitated)

D ay 1 b D ay 2 D ay 7 D ays 8

- 14

Vehicle Control 10 0 0 0 0

125 10 0 (1) 0 1 1

250 10 0 (1) 0 1 1

500 10 0 (7) 0 1 1

1000 10 0 (7) 1 1 1

2000 10 5 (6) 8 8 8

NOAEL <125 mg/ kg bw

LD 50 (with 95% C.I.) Based on

Mortalities 1560 ( 929

- 5660 ) mg/ kg bw

LD 50 (with 95% C.I.) Based on

Regurgitation 39 7 (268

– 588 ) mg/ kg bw

a Data obtained from Table 1 on page 2 6 of study report.

b Mortalities occurred within 22 hours after dosing on Day 1.

B. SUBLETHAL T OXICITY ENDPOINTS:

According to the study authors, n o clinical signs of toxicity were observed in the control

. Signs of toxicity were

observed in all treatment group s and included ruffled feathers, underactivity, incoordination, recumbency, body

tremors, panting, salivation and/or r egurgitation

. Regurgitation was observed within one hour of dosing for 1, 1,

7, 7, and 6 birds in the 125 , 250, 500, 1000, and 2000 mg/ kg groups, respectively ( Table 4 )

. Across all dose

levels, 12 male and 10 female birds regu rgitated.

The study authors re ported that there were no apparent treatment

- related effects upon body weight or body

weight change at any interval for males or females at any treatment group compared to the control over the 14

- day study period

. No signifi cant treatment

- related macroscopic abnormalities were observed at necropsy.

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 8 of 13

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 9 of 13 Table 4: Reported Sublethal Effects of Tribufos in the Zebra F inch

. a

Mean Body Weight (and Change) , g

Treatment,

( mg/ kg bw) Males Females

Day 0 Day 7 Day 14 Day 0 Day 7 Day 14

Vehicle Control 15.6 16.2

(0.6) 16.3

(0.1) 14. 4 15.3

(0.9) 15.7

(0.4)

125 16.1

(16.9) 17.7 c(4)

(0.8 ) 18.1 c(4)

( 0.4 ) 14.3 14.9

(0.6 ) 15.3

( 0.4 )

250 14.2 14.9

(0.7 ) 15.1

(0.2 ) 15.6

(15.3) 15.8 c(4)

(0.5 ) 16.5 c(4)

(0.7 )

500 20.0

(20.1) 19.5 c(4 )

(

- 0.6 ) 19.8 c(4)

( 0.3 ) 16.0 16.2

(0.2 ) 16.5

(0.3 )

1000 15.1 15.0

(

- 0.1 ) 15.2

( 0.2 ) 16.1

(16.6) 17.1 c (4)

( 0.5 ) 18.2 c (4)

( 1.1 )

2000 17.3

(16.7) 15.6 c(1)

(

- 1.1 ) 17.2 c(1)

( 1.6 ) 18.0

(22.9) 22.1 c(1)

(

- 0.8 ) 21.9 c(1)

(

- 0.2 )

NOAEL 2000 mg/ kg 2000 mg/ kg

EC 50 Not reported Not reported

Mean Feed Consumption, g/bird/day

Treatment,

( mg/ kg bw) Males Females

Day s 1

- 7 Day s 8

- 14 Day s 1

- 7 Day s 8

- 14

Vehicle Control 8.8 8.5 10.5 9.1

125 6.6 7.7 6.1 6.6

250 7.1 8.0 7.0 7.5

500 8.9 8.4 6.3 5.5

1000 7.3 6.9 6.7 7.7

2000 6.7 7.1 7.0 7.4

NOAEL 2000 mg/ kg 2000 mg/ kg

EC 50 Not reported Not reported

a Data obtained from Tables 3 and 4 on pages 28

- 29 of study report.

b The Day 0 mean body weights ( in parentheses ) used for calculating bodyweight changes are adj usted based on surviving birds.

c Based on fewer than five birds (number surviving birds).

NA=Not applicable , due to 100% mortality

.

C. REPORTED STATISTICS:

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 10 of 13 The LD 50 , slope, and 95% confidence interval values were calculated by the study authors usin g logistic regression

in the SAFEStat computer program (version 1.5)

. No statistical analyses were applied to separate mean responses

among treatment groups for body weight or food consumption data.

The study author calculated a second LD 50 value based on “theoretical maximum mortality,” counting all birds that

regurgitated as mortalities. This calculation, using logistic regression, results in an LD 50 of 393 mg/ kg bw (95% CI:

229 to 630) with a slope of 1.5

D. VERIFICATION OF STATISTICAL RESULTS:

St atistical Method : The LD 50 value was calculated by the reviewer using the Trimmed Spearman

- K ä rber (TSK)

method via CETIS version 1.8.7. 12 statistical software with backend database settings implemented by EFED on 29

May 2013. TSK was used because the data did not meet assumptions of normality required for probit regression

(Shapiro

- Wilk’s test, p=0.01). The body weight change and standard error data were entered into CETIS but not

analyzed. NOAEC values were not calculated. The results are reported in te rms of nominal concentrations, as

measured concentrations were not provided.

Slope: Not Applicable 95% C.I.: Not Applicable

Endpoint(s) affected : Mortality and signs of toxicity (including regurgitation)

E. STUDY DEFICIENCIES:

The test material was regurgitated within one hour of dosing by at least one bird from every treated level, with

increasing incidents of regurgitation at higher doses

. There were no other deficiencies from OCSPP 850.2100

(2012) guidance were noted

.

F. REVIEWER ’ S COMMENTS:

The reviewer’s L D 50 estimate was lower with a narrower 95% confidence interval than that reported by the study

author. T he reviewer’s results are reported in the Executive Summary and Conclusions sections of this DER

. However, the actual dose levels we re not measured.

Regurgitation w as observed within one hour of dosing in all treatment groups. Because the birds regurgitated

following dosing, actual exposure to the test compound is uncertain . Therefore, the true LD 50 is likely lower than

the value

- initially calculated by the study authors

. Due to the resulting uncertainty regarding actual exposure

levels the study endpoint, this study deficiency affect s the scientific soundness and acceptability of this study.

In

- life study dates were December 5

- 19 , 2012

.

G. CONCLUSIONS:

This study is classified as supplemental due to observed regurgitation, which increases uncertainty i n actual

exposure levels and the resulting LD 50 estimate

. Treatment

- related mortalit y of 10, 10, 10, 10, and 80% w as

observ ed at 125, 250, 500, 1000, and 2000 mg/ kg bw groups, respectively

. Signs of toxicity were observed in

all treatment groups and included ruffled feathers, underactivity, incoordination, recumb e ncy, body tremors,

panting, salivation and/or regurgitation

. R egurgitation was observed within one hour of dosing for 1, 1, 7, 7,

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 11 of 13 and 6 birds in the 125 , 250, 500, 1000, and 2000 mg/ kg groups, respectively. There were no apparent

treatment

- related effects upon body weight or body weight change at any interval for ma les or females at any

treatment group compared to the control ; however, based on regurgitation observed in each of the treatment

groups, the NOAEL is less than the lowest treatment level (< 125 mg/ kg bw)

. The reviewer calculated the acute

oral LD 50 as 39 7 mg/

kg with 95% confidence interval of 2 68 to 588 mg/ kg

.

LD 50 : 397 mg/

kg bw 95% C.I.: 268 to 588 mg/ kg bw

Endpoint(s) a ffected : Mortality and signs of toxicity (including regurgitation)

III. REFERENCES :

None; other than standard guidelines or methodologies.

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 12 of 13 APPENDIX I. OUTPUT OF REVIEWER’S STATISTICAL VERIFICATION: Calculation of % weight gain and

comparison of % weight gain between the control and treated groups using Microsoft Excel



### Male Female

Dose Day 0 Day 14 Chang e Day 0 Day 14 Change

0 16.8 17.9 1.1 13.3 14.1 0.8

13.3 13.9 0.6 15.1 16.1 1

17.1 17.4 0.3 13.3 14.8 1.5

15.4 16.9 1.5 13.8 15.4 1.6

15.2 15.6 0.4 16.6 18.3 1.7

Mean 15.6 16.3 0.78 14.4 15.7 1.32

St. Dev. 1.3544 1.440278 0.453431 1.2734 21 1.440278 0.354401

St. Error 0.605706 0.644112 0.20 0.569491 0.644112 0.16

125 17.5 18.4 0.9 14.8 17.4 2.6

17 18.1 1.1 14.1 14.9 0.8

15.4 17.1 1.7 15.1 16.2 1.1

13.1 15.5 15.8 0.3

17.5 18.6 1.1 12.2 12.1

- 0.1

Mean 16. 1 18.1 1.20 14.3 15.3 0.94

St. Dev. 1.686416 0.576628 0.3 1.163787 1.78146 0.926499

St. Error 0.754188 0.288314 0.15 0.520461 0.796693 0.41

250 12.7 12.9 0.2 12.8 13.7 0.9

16.2 17.5 1.3 12.4 13.4 1

14.1 15.2 1.1 13.1 14.1 1

12.8 13.6 0.8 17

15.1 16.2 1.1 22.7 24.7 2

Mean 14.2 15.1 0.90 15.6 16.5 1.23

St. Dev. 1.343726 1.677379 0.384708 3.916631 4.755195 0.449305

St. Error 0.600933 0.750147 0.17 1.751571 2.377597 0.22

500 13.6 14.5 0.9 16.8 17. 5 0.7

25.9 24.5

- 1.4 13.6 13.7 0.1

### Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch

( Taeniopygia guttata )

EPA MRID Numbe r 49121302

Page 13 of 13 

### 20.9 20.6

- 0.3 14.1 14.3 0.2

19.8 19.6

- 0.2 15.9 16.1 0.2

19.7 19.4 20.8 1.4

Mean 20.0 19.8

- 0.25 16.0 16.5 0.52

St. Dev. 3.916835 3.56581 0.813941 2.077113 2.542754 0.487442

St. Error 1.751662 1.782905 0.41 0.928913 1.137154 0.22

1000 14.9 15.4 0.5 20.8 21.7 0.9

19.3 16.9

- 2.4 14.1

14.5 15.2 0.7 16.4 20.3 3.9

13.1 14.9 1.8 12.9 14.3 1.4

13.7 13.8 0.1 16.1 16.4 0.3

Mean 15.1 15.2 0.14 16.1 18.2 1.63

St. Dev. 2.19089 0.997196 1.389388 3.017118 3.420892 1.581929

St. Error 0.979796 0.44596 0.62 1.349296 1.710446 0.791

2000 16.1 14.9

16.7 22.9 22.1

- 0.8

16.7 15.6

- 1.1 16

18.3 19.6

18.5 16.5

Mean 17.3 15.6

- 1.1 18.0 22.1

- 0.80

St. Dev. 0.958332 0 0 2.913005 0 0

St. Error 0.428579 0 0.000 1.302736 0 0.00

### CETIS Summary Report Report Date: 14 Nov-13 08:52 (p 1 of 1)

Test Code: 074801 49121302 | 04-3500-2085

OCSPP 850.2100 Acute Avian Oral Toxicity Huntingdon Life Sciences

Batch ID: 07-6221-9965

Start Date: 05 Dec-12

Ending Date: Test Type: Acute Avian Oral Toxicity

Duration: NA Protocol: OCSPP 850.2100 Acute Bird Diluent: Corn Oil

Brine: Not Applicable Species: Taeniopygia guttata

Source: Lab In-House Culture Analyst:

Age: <10m

Sample ID: 01-2835-0091

Sample Date: 05 Dec-12

Receive Date: Code: 49121302

Sample Age: NA Source: AMVAC Chemical Corporation

Station: Client: CDM Smith

Project: Material: Tribuphos

PC Code 074801, MRID 49121302

PC Code 074801 MRID 49121302 Batch Note:

Sample Note:

95% LCL 95% UCL Point Estimate Summary

Endpoint Analysis ID Method mg ai/kgB TU Level

LC5 1070 N/A N/A 14dMortalityRate 15-6993-1879 Linear Regression (MLE)

LC10 1180 N/A N/A

LC15 1260 N/A N/A

LC20 1320 N/A N/A

LC25 1380 N/A N/A

LC40 1530 N/A N/A

LC50 1640 N/A N/A

LC50 1490 1260 1750 14dMortalityRate 17-1363-5181 Trimmed Spearman-Kärber

Control Type Mean Min Max Std Dev

Count CV% Std Err C-mg ai/kgB 14dMortalityRate Summary

95% LCL 95% UCL %Effect

0 Negative Control 0 0 0 0 1 0

125 0.1 0.1 0.1 0 1 0.0% 0

250 0.1 0.1 0.1 0 1 0.0% 0

500 0.1 0.1 0.1 0 1 0.0% 0

1000 0.1 0.1 0.1 0 1 0.0% 0

2000 0.8 0.8 0.8 0 1 0.0% 0

CETISTM v1.8.7.12 000-516-187-1 QA:________ Analyst:________

### CETIS Summary Report Report Date: 14 Nov-13 08:52 (p 1 of 1)

Test Code: 074801 49121302 | 04-3500-2085

OCSPP 850.2100 Acute Avian Oral Toxicity Huntingdon Life Sciences

Batch ID: 07-6221-9965

Start Date: 05 Dec-12

Ending Date: Test Type: Acute Avian Oral Toxicity

Duration: NA Protocol: OCSPP 850.2100 Acute Bird Diluent: Corn Oil

Brine: Not Applicable Species: Taeniopygia guttata

Source: Lab In-House Culture Analyst:

Age: <10m

Sample ID: 01-2835-0091

Sample Date: 05 Dec-12

Receive Date: Code: 49121302

Sample Age: NA Source: AMVAC Chemical Corporation

Station: Client: CDM Smith

Project: Material: Tribuphos

PC Code 074801, MRID 49121302

PC Code 074801 MRID 49121302 Batch Note:

Sample Note:

95% LCL 95% UCL Point Estimate Summary

Endpoint Analysis ID Method mg ai/kgB TU Level

LC5 1070 N/A N/A 14dMortalityRate 15-6993-1879 Linear Regression (MLE)

LC10 1180 N/A N/A

LC15 1260 N/A N/A

LC20 1320 N/A N/A

LC25 1380 N/A N/A

LC40 1530 N/A N/A

LC50 1640 N/A N/A

LC50 1490 1260 1750 14dMortalityRate 17-1363-5181 Trimmed Spearman-Kärber

Control Type Mean Min Max Std Dev

Count CV% Std Err C-mg ai/kgB 14dMortalityRate Summary

95% LCL 95% UCL %Effect

0 Negative Control 0 0 0 0 1 0

125 0.1 0.1 0.1 0 1 0.0% 0

250 0.1 0.1 0.1 0 1 0.0% 0

500 0.1 0.1 0.1 0 1 0.0% 0

1000 0.1 0.1 0.1 0 1 0.0% 0

2000 0.8 0.8 0.8 0 1 0.0% 0

CETISTM v1.8.7.12 000-516-187-1 QA:________ Analyst:________