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                            Funding & Awards
 Simple, Rapid, and Accurate Quantitation of Human DNA
Note:
              This awardee has received supplemental funding. This award detail page includes information about both the original award and supplemental awards.
Award Information
Awardee
              Vermont Department of Public Safety
Award #
              2000-IJ-CX-K012
Funding Category
              Continuation
Location
                    Waterbury, VT
Status
              Closed
Funding First Awarded
              2000
Total funding (to date)
              $384,132
Original Solicitation
              Enhancing DNA ID Capabilities for Reduction of Violent Crime
Description of original award (Fiscal Year 2000, $67,129)
Project summary for 2000-IJ-CX-K012 (S-2)

This project is a supplement to award 2000-IJ-CX-K012, and focuses on the continued development and optimization of methods for dtermining the amount of human DNA in a biological evidentiary sample. The forensic community has embraced DNA profiling for the analysis of biological evidence. Although a number of DNA profiling methods exist, that analysis of short tandem repeats (STRs) has become the standard within the community. There are a number of steps required to obtain an STR profile from a crime scene sample. One critical step is the determination of the amount of human DNA present in the sample. The forensic community relies almost entirely upon a technique that is inherently inaccurate, time-consuming and labor-intensive. The goal of this project is to evaluate and optimize alternative approaches to human DNA quantitation in order to increase the accuracy, expand the analytical range, and make the procedure less labor-intensive.

This phase of the project will explore various DNA quantitation strategies, all focusing on the detection of the high-copy number, primate-specific Alu sequences in human DNA. From among the methods, one or two will be chosen for optimization in terms of cost, reliability, and analyst time. The method(s) will be validated by comparison with the slot blot method on mock and real forensic samples. The information obtained from this study will then be disseminated to the forensic DNA community.


nca/ncf
Supplemental Award 1 (Fiscal Year 2000, $124,970)
Project summary for 2000-IJ-CX-K012 (S-2)
This project is a supplement to award 2000-IJ-CX-K012, and focuses on the continued development and optimization of methods for dtermining the amount of human DNA in a biological evidentiary sample. The forensic community has embraced DNA profiling for the analysis of biological evidence. Although a number of DNA profiling methods exist, that analysis of short tandem repeats (STRs) has become the standard within the community. There are a number of steps required to obtain an STR profile from a crime scene sample. One critical step is the determination of the amount of human DNA present in the sample. The forensic community relies almost entirely upon a technique that is inherently inaccurate, time-consuming and labor-intensive. The goal of this project is to evaluate and optimize alternative approaches to human DNA quantitation in order to increase the accuracy, expand the analytical range, and make the procedure less labor-intensive.
This phase of the project will explore various DNA quantitation strategies, all focusing on the detection of the high-copy number, primate-specific Alu sequences in human DNA. From among the methods, one or two will be chosen for optimization in terms of cost, reliability, and analyst time. The method(s) will be validated by comparison with the slot blot method on mock and real forensic samples. The information obtained from this study will then be disseminated to the forensic DNA community.
nca/ncf
    Supplemental Award 2 (Fiscal Year 2000, $192,033)
Project summary for 2000-IJ-CX-K012 (S-2)
This project is a supplement to award 2000-IJ-CX-K012, and focuses on the continued development and optimization of methods for dtermining the amount of human DNA in a biological evidentiary sample. The forensic community has embraced DNA profiling for the analysis of biological evidence. Although a number of DNA profiling methods exist, that analysis of short tandem repeats (STRs) has become the standard within the community. There are a number of steps required to obtain an STR profile from a crime scene sample. One critical step is the determination of the amount of human DNA present in the sample. The forensic community relies almost entirely upon a technique that is inherently inaccurate, time-consuming and labor-intensive. The goal of this project is to evaluate and optimize alternative approaches to human DNA quantitation in order to increase the accuracy, expand the analytical range, and make the procedure less labor-intensive.
This phase of the project will explore various DNA quantitation strategies, all focusing on the detection of the high-copy number, primate-specific Alu sequences in human DNA. From among the methods, one or two will be chosen for optimization in terms of cost, reliability, and analyst time. The method(s) will be validated by comparison with the slot blot method on mock and real forensic samples. The information obtained from this study will then be disseminated to the forensic DNA community.
nca/ncf


  
Grant-Funded Publications
        
                      Simple, Rapid, and Accurate Quantitation of Human DNA
                  Alu-based, MGB Eclipse(TM) Real-Time PCR Method for Quantitation of Human DNA in Forensic Samples

  
Date Created: September 29, 2000
    
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