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                            Funding & Awards
 SPERM CELL SELECTION SYSTEM FOR FORENSIC DNA OF THE MALE COMPONENT
Note:
              This awardee has received supplemental funding. This award detail page includes information about both the original award and supplemental awards.
Award Information
Awardee
              University of Virginia
Award #
              2000-IJ-CX-K013
Funding Category
              Continuation
Location
                    Charlottesville, VA
Awardee County
              Albemarle
Congressional District
              5
Status
              Closed
Funding First Awarded
              2004
Total funding (to date)
              $644,591
Original Solicitation
              National Institute of Justice Continuation Awards - Office of Science and Technology
Description of original award (Fiscal Year 2004, $305,532)
Project summary for 2000-IJ-CX-K013
THe objective of this research project is to develop a sperm cell selection device suitable for forensic DNA analysis of the "male component" in sexual assault evidence. The device and its companion mehthodologies will be compatible with high throughput automation employing PCR of short tandem repeat loci. The device will provide an enabling technology to assist the development of the Combined DNA Index System (CODIS).

Monoclonal antibodies and recombinant mini-antibodies will produced against a unique human sperm surface carbohydrates epitope,sperm agglutination antigen-1(SAGA-1).This antigen is synthesized in the principal cells of the epididymis, is specific to the male reproductive trac of humans and higher primates and is inserted by way of a GPI anchor into all domains of the human sperm surface: Head,midpiece and tail. This antigen thus offers an excellent target for developing methods for separating sperm
cells from other biological materials.
Immuno-reagents to SAGA-1 will be coupled to magnetic beads and magnetite particles.Methodologies employing these prototype devices, including single sperm PCR, will be developed and standardized to yield differential separation of male germ cell DNA from DNA of other cell types.
Supplemental Award 1 (Fiscal Year 2004, $339,059)
The microscopic identification of sperm in sexual assault evidence can be labor intensive and time consuming.  During the initial phase of this project, fluorescently labeled anti-sperm antibodies were developed.  These antibodies (called "Sperm Paints") have the capability to bind to sperm and make them easily visible using a fluorescent microscope. The ability of the Sperm Paints to bind to and illuminate sperm heads and tails in simulated sexual assault evidence that has been stored for prolonged periods has also been demonstrated.  The goal of this supplemental funding is to complete the development and optimization of a protocol for using the Sperm Paints for microscopic sperm searches, and to perform testing and validation of the Sperm Paints through collaborative studies with crime labs.
Objectives:
1. Optimize sperm binding by a cocktail of fluorescent monoclonal anti-sperm antibodies (Sperm Paint) which bind to sperm specific proteins located in different domains of the sperm head and tail.
2.  Determine the binding characteristics of Sperm Paint to simulated evidence obtained 24, 48, 72 and 96 hours post-coitus.
3. Collaborate with crime labs to evaluate and validate the Sperm Paints.
4.  Develop an optimized protocol for Sperm Paint use.
ca/ncf


  
Grant-Funded Publications
        
                      SpermPaint Optimization and Validation
                  Radial Spoke Protein 44 (Human Meichroacidin) is an Axonemal Alloantigen of Sperm and Cilia
                  SAMP14, a Novel, Acrosomal Membrane-associated,Glycosylphosphatidylinositol-Anchored Member of the Ly-6/Urokinase-Type Plasminogen Activator Receptor Superfamily With a Role in Sperm-Egg Interaction
                  SLLP1, A Unique, Intra-Acrosomal, Non-Bacteriolytic, C Lysozyme-like Protein of Human Spermatozoa
                  Male Genital Tract-Specific Carbohydrate Epitope on Human CD52: Implications for Immunocontraception
                  RASA, A Recombinant Single-Chain Variable Fragment (scFv) Antibody Directed Against the Human Sperm Surface: Implications for Noval Contraceptives

  
Date Created: September 29, 2000
    
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