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                            Funding & Awards
 Separation of Sperm and Epithelial Cells in a Microfluidic Device: An Automated Method for High Efficiency, High Purity Separations.
Award Information
Awardee
              Rector and Visitors of the University of Virginia
Award #
              2006-DN-BX-K021
Funding Category
              Competitive
Location
                    Charlottesville, VA
Congressional District
              5
Status
              Closed
Funding First Awarded
              2006
Total funding (to date)
              $324,104
Original Solicitation
              NIJ FY06 Forensic DNA Research and Development Solicitation: Invited Full Applications
Description of original award (Fiscal Year 2006, $324,104)
The aim of this proposal is to produce a microfabricated device method for obtaining male and female fractions of DNA in a fast, automated, low-cost manner that will provide improved efficiency and purity in comparison with conventional differential extraction. The researcher will use acoustic differential extraction (ADE), which has been demonstrated as a method for trapping of sperm cells in a microchannel. This process will then be integrated, on a single device, with a solid phase extraction process to obtain purified DNA from the male and female fractions. The integrated device will accept sperm cell/epithelial cell lysate mixtures produced with the first step (mild lysis) of the current extraction protocol and trap the sperm cells, while collecting the epithelial cell DNA. The sperm cells will then be lysed and the DNA from both fractions separately purified on-chip.
ca/ncf



  
Grant-Funded Publications
        
                      Acoustic Differential Extraction for Forensic Analysis of Sexual Assault Evidence
                  Separation of Sperm and Epithelial Cells in a Microfluidic Device: An Automated Method for High Efficiency, High Purity Separations

  
Date Created: September 12, 2006
    
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