Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 1 of 13

Data Requirement: EPA DP Barcode D412314

EPA MRID 491213-02

EPA Guideline 850.2100 (OCSPP)

Test material: Tribufos Technical Purity: 98.2%

Common name: Tribufos

Chemical name: IUPAC: S,S,S-tributyl phosphorotrithioate

CAS: S,S,S-tributyl phosphorotrithioate

CAS No.: 78-48-8

Primary Reviewer: Rebecca Bryan Signature: 

Staff Scientist, CSS-Dynamac Corporation Date: 10/10/13

Secondary Reviewer: John Marton, Ph.D. Signature: 

Environmental Scientist, CDM Smith Date: 11/14/13 

Secondary Reviewer(s): Mary Clock-Rust Signature: 

Biologist, ERB4, EFED, OPP, OCSPP, EPA Date: 1/14/15

EPA PC Code 074801

Date Evaluation Completed: 01-14-2015

CITATION: Dias, N.A. 2013. Tribufos: Acute Oral Toxicity (LD50) to the Zebra Finch. Unpublished study 

performed by Huntingdon Life Sciences, Ltd., Huntingdon, Cambridgeshire, England. Laboratory Study No. 

BDG0143. Study sponsored by AMVAC Chemical Corporation, Newport Beach, CA. Study initiated June 18, 

2012 and completed April 15, 2013. 
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 2 of 13

EXECUTIVE SUMMARY:

The acute oral toxicity of tribufos to 8- to 10-month old zebra finches (Taeniopygia guttata) was assessed over 14 days. 

Tribufos was administered to the birds by oral gavage at nominal levels of 0 (vehicle control, corn oil), 125, 250, 

500, 1000, and 2000 mg/kg bw. Treatment-related mortality of 10, 10, 10, 10, and 80% was observed at 125, 250, 

500, 1000, and 2000 mg/kg bw groups, respectively. Signs of toxicity were observed in all treatment groups and 

included ruffled feathers, underactivity, incoordination, recumbency, body tremors, panting, salivation and/or emesis

(regurgitation). Regurgitation was observed within one hour of dosing for 1, 1, 7, 7, and 6 birds in the 125, 250, 500, 

1000, and 2000 mg/kg groups, respectively. There were no apparent treatment-related effects upon body weight or 

body weight change at any interval for males or females at any treatment group compared to the control over the 14-

day study period. 

Because the birds regurgitated after dosing, actual exposure is likely lower than the nominal dose. Therefore, the 

LD50 may be lower than that (LD50 = 1560 mg/kg bw) calculated by the study authors. Due to the resulting 

uncertainty in exposure as well as the study endpoint, these study deficiencies affect the scientific soundness and 

acceptability of this study. Assuming that each of the birds that regurgitated represents a mortality in addition to 

those that actually died, the 14-day LD50 is estimated at 397 mg/kg bw. Based on this value, tribufos would be 

classified as slightly toxic to birds on an acute oral exposure basis. Since regurgitation was observed in each of the 

treatment groups a definitive NOAEL was not determined, i.e., NOAEL<125 mg/kg bw. This study is classified as 

supplemental due to observed regurgitation.

Results Synopsis

Test Organism Size/Age (Weight range): Adult, 22 weeks old; 14.3 to 24.0 g

LD50: 397 mg/kg bw 95% C.I.: 268-588 mg/kg bw

Endpoint(s) affected: Mortality and signs of toxicity (including regurgitation)

 
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 3 of 13

I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The study protocol was based upon procedures outlined in U.S. EPA 

Ecological Effects Test Guidelines, OCSPP No. 850.2100, Avian Acute 

Oral Toxicity Test (2012).

Deviations from OCSPP 850.2100 guidance included:

 Concentrations of test substance in the dosing solutions were not analytically-confirmed.

 One or more birds at all treated levels regurgitated test material after one hour of dosing. 

These deviations affect the scientific soundness of this study and the study classification.

COMPLIANCE: Signed and dated GLP, Quality Assurance, and Data Confidentiality 

statements were provided. 

A. MATERIALS:

1. Test material: Tribufos technical

Description: Liquid

Lot No./Batch No. : NX81AX0015

Purity: 98.2% 

Stability of compound 

under test conditions: Not reported.

 

Storage conditions of 

test chemicals: Room temperature desiccated.

Physicochemical properties of Tribufos.

Parameter Values Comments

Water solubility at 20°C 2.3 mg/L MRID 41618803

Vapor pressure at 20°C 1.7 x 10-6

torr MRID 41618803

Structure

Kow at 20°C 3.31 x 105 MRID 41618803

(OECD recommends water solubility, stability in water and light, pKa, Pow, and vapor pressure of test compound)

2. Test Organism:
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302 

Page 4 of 13

Species (common and scientific names): Zebra finch (Taeniopygia guttata) 

Age at study initiation: Adult, 8 to 10 months old 

Weight at study initiation (range): 12.7 to 25.2 g 

Source: Huntingdon Life Sciences stock 

B. STUDY DESIGN:

1. Experimental Conditions 

a. Range-finding study: The definitive test concentrations were based on a range-finding study using 10 

birds dosed at 400, 650, 900, and 1500 mg/kg. Signs of toxicity were observed in all treatment groups and 

included minor clinical signs (not described), transiently wiping its beak, underactive, shaking its head, 

ruffled feathers, body tremors, panting, and/or recumbency. 

b. Definitive study: 

Table 1: Experimental Parameters

Parameter Details Remarks

Criteria

Acclimation 

Period:

Conditions: (same as test or not)

Feeding:

Health: (any mortality observed)

14 days

Same as test

A proprietary seed mixture

and water were 

available ad libitum. 

Birds in apparent good health.

The recommended acclimation period is 

a minimum of 15 days. OECD 

recommends a minimum of 7 days.

Pen size and construction materials: 75 x 45.5 x 46 cm

Constructed of powder-coated wire 

mesh. Pen size and construction should 

conform to good husbandry practices 

and should not create crowding stress.

OECD recommends that pens be 

suitable for the captive rearing of that 

species.

Test duration: 14 days

Recommended test duration is one day 

for dosing and at least 14 days 

observation.
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 5 of 13

Parameter Details Remarks

Criteria

Dose preparation [Indicate method 

of confirmation of dose] The appropriate amounts of test 

substance were dissolved/dispersed

in corn oil. 

Confirmation of dosing was not 

assessed.

Mode of dose administration: Gavage at 5 mL/kg dose volume. 

Gavage or gelatin capsule is 

recommended

Dose levels

Nominal:

Measured:

0 (vehicle control), 125, 250, 500, 

1000, and 2000 mg/kg bw

Not determined

Dose levels should be a minimum of 5 

treatment levels unless LD50 is 

demonstrated to be greater than 2000 

mg/kg

Solvent/vehicle, if used

Type:

Amount/bw:

Corn oil

Not reported The test material should be administered 

without a vehicle if possible. Maximum 

vehicle should not exceed 0.1 to 1.0% of 

body weight.

Number of birds per group/treatment

Negative control:

Solvent/vehicle control:

Treated:

N/A

10 (5 per sex)

10 (5 per sex) Recommended number of birds in a 

treatment group is 10 and 10 birds for 

each control and vehicle group.

No. of feed withholding days before 

dosing: At least 3 to 4 hours

The feed was withheld for 3-4 hours 

instead of the recommended 15 hours.

Food should be withheld for at least 15 

hours prior to dosing.

Test conditions

Temperature:

Relative humidity:

Photoperiod:

16-22°C

38-58%

10 hours light/14 hours dark

The recommended photoperiod is 10 

hours of light and 14 hours of dark.

Reference chemical, if used

Name:

Concentrations tested:

None tested
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 6 of 13

2. Observations: 

Table 2: Observations

Criteria Details Remarks

Criteria

Parameters measured

(mortality/individual body weight at 

test initiation and termination/ mean 

feed consumption/ others) 

- Mortality

- Clinical signs of toxicity

- Body weight

- Food consumption

Body weight should be measured at test 

initiation, on day 14 and at the end of 

the test if the test is extended beyond 14 

days. Mortality should not be more than 

10% in controls.

Feed consumption should be measured 

as average daily food consumption.

Indicate if the test material was 

regurgitated

Birds were observed regurgitating 

within one hour of dosing in all 

treatment groups. Regurgitation 

(emesis) was observed for 1, 1, 7, 7, 

and 6 birds in the 125, 250, 500, 

1000, and 2000 mg ai/kg groups, 

respectively (12 males and 10 

female).

Regurgitation is an indication that the 

dose was rejected. If this problem 

persists, the test should be repeated. 

Groups on which necropsies were 

performed

Necropsies were performed on all 

birds that died or were sacrificed

during test. In addition, all control 

birds and ten surviving treated birds 

from the highest dose groups with 

survivors were necropsied.

Gross necropsies should be performed 

with inspections of the GI tract, liver, 

kidneys, heart, and spleen.

Observation intervals: Birds were observed for mortality 

and clinical signs of toxicity 

continuously for one hour 

immediately after dosing and twice 

daily thereafter. 

Individual body weights were 

measured individually on Days 0, 7, 

and 14. 

Food consumption was estimated 

for Day -1, Days 1-7, and Days 8-

14.

Were raw data included? Yes
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 7 of 13

II. REPORTED RESULTS AND DISCUSSION:

A. MORTALITY:

No mortalities were observed in the control. According to the study authors, treatment-related mortalities of 10, 

10, 10, 10, and 80% were observed at 125, 250, 500, 1000, and 2000 mg/kg bw groups, respectively. 

According to the study author, the acute oral LD50 was calculated as 1560 mg/kg bw with 95% confidence 

interval of 929 to 5660 mg/kg bw (Table 3). Assuming that each of the birds that regurgitated represents a 

mortality in addition to those that actually died, the 14-day LD50 is estimated at 397 mg/kg bw. The NOAEL 

was not determined based on mortalities in all treatment groups. 

Table 3: Reported Effect of Tribufos on Mortality in the Zebra Finch.

a

 

Treatment

(mg/kg bw)

No. of 

Birds

Cumulative Mortality (birds that regurgitated)

Day 1 b Day 2 Day 7 Days 8-14

Vehicle Control 10 0 0 0 0

125 10 0(1) 0 1 1

250 10 0 (1) 0 1 1

500 10 0 (7) 0 1 1

1000 10 0 (7) 1 1 1

2000 10 5 (6) 8 8 8

NOAEL <125 mg/kg bw

LD50 (with 95% C.I.) Based on 

Mortalities

1560 (929-5660) mg/kg bw

LD50 (with 95% C.I.) Based on 

Regurgitation

397 (268 – 588) mg/kg bw

a Data obtained from Table 1 on page 26 of study report. 

b Mortalities occurred within 22 hours after dosing on Day 1.

B. SUBLETHAL TOXICITY ENDPOINTS:

According to the study authors, no clinical signs of toxicity were observed in the control. Signs of toxicity were 

observed in all treatment groups and included ruffled feathers, underactivity, incoordination, recumbency, body 

tremors, panting, salivation and/or regurgitation. Regurgitation was observed within one hour of dosing for 1, 1, 

7, 7, and 6 birds in the 125, 250, 500, 1000, and 2000 mg/kg groups, respectively (Table 4). Across all dose 

levels, 12 male and 10 female birds regurgitated.

The study authors reported that there were no apparent treatment-related effects upon body weight or body 

weight change at any interval for males or females at any treatment group compared to the control over the 14-

day study period. No significant treatment-related macroscopic abnormalities were observed at necropsy.
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 8 of 13
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 9 of 13

Table 4: Reported Sublethal Effects of Tribufos in the Zebra Finch.

a

Mean Body Weight (and Change), g 

Treatment,

(mg/kg bw)

Males Females

Day 0 Day 7 Day 14 Day 0 Day 7 Day 14

Vehicle Control 15.6 16.2

(0.6)

16.3

(0.1)

14.4 15.3

(0.9)

15.7

(0.4)

125 16.1

(16.9)

17.7 c(4)

(0.8)

18.1 c(4)

(0.4)

14.3 14.9

(0.6)

15.3

(0.4)

250 14.2 14.9

(0.7)

15.1

(0.2)

15.6

(15.3)

15.8 c(4)

(0.5)

16.5 c(4)

(0.7)

500 20.0

(20.1)

19.5 c(4)

(-0.6)

19.8 c(4)

(0.3)

16.0 16.2

(0.2)

16.5

(0.3)

1000 15.1 15.0

(-0.1)

15.2

(0.2)

16.1

(16.6)

17.1 c(4)

(0.5)

18.2 c(4)

(1.1)

2000 17.3

(16.7)

15.6 c(1)

(-1.1)

17.2 c(1)

(1.6)

18.0

(22.9)

22.1 c(1)

(-0.8)

21.9 c(1)

(-0.2)

NOAEL 2000 mg/kg 2000 mg/kg

EC50 Not reported Not reported

Mean Feed Consumption, g/bird/day

Treatment,

(mg/kg bw)

Males Females

Days 1-7 Days 8-14 Days 1-7 Days 8-14

Vehicle Control 8.8 8.5 10.5 9.1

125 6.6 7.7 6.1 6.6

250 7.1 8.0 7.0 7.5

500 8.9 8.4 6.3 5.5

1000 7.3 6.9 6.7 7.7

2000 6.7 7.1 7.0 7.4

NOAEL 2000 mg/kg 2000 mg/kg

EC50 Not reported Not reported

a Data obtained from Tables 3 and 4 on pages 28-29 of study report. 

b The Day 0 mean body weights (in parentheses) used for calculating bodyweight changes are adjusted based on surviving birds.

c Based on fewer than five birds (number surviving birds).

NA=Not applicable, due to 100% mortality.

C. REPORTED STATISTICS:
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 10 of 13

The LD50, slope, and 95% confidence interval values were calculated by the study authors using logistic regression 

in the SAFEStat computer program (version 1.5). No statistical analyses were applied to separate mean responses 

among treatment groups for body weight or food consumption data. 

The study author calculated a second LD50 value based on “theoretical maximum mortality,” counting all birds that 

regurgitated as mortalities. This calculation, using logistic regression, results in an LD50 of 393 mg/kg bw (95% CI: 

229 to 630) with a slope of 1.5

D. VERIFICATION OF STATISTICAL RESULTS:

Statistical Method: The LD50 value was calculated by the reviewer using the Trimmed Spearman-Kärber (TSK) 

method via CETIS version 1.8.7.12 statistical software with backend database settings implemented by EFED on 29 

May 2013. TSK was used because the data did not meet assumptions of normality required for probit regression 

(Shapiro-Wilk’s test, p=0.01). The body weight change and standard error data were entered into CETIS but not 

analyzed. NOAEC values were not calculated. The results are reported in terms of nominal concentrations, as 

measured concentrations were not provided.

Slope: Not Applicable 95% C.I.: Not Applicable

Endpoint(s) affected: Mortality and signs of toxicity (including regurgitation)

E. STUDY DEFICIENCIES:

The test material was regurgitated within one hour of dosing by at least one bird from every treated level, with 

increasing incidents of regurgitation at higher doses. There were no other deficiencies from OCSPP 850.2100 

(2012) guidance were noted. 

F. REVIEWER’S COMMENTS: 

The reviewer’s LD50 estimate was lower with a narrower 95% confidence interval than that reported by the study 

author. The reviewer’s results are reported in the Executive Summary and Conclusions sections of this DER. 

However, the actual dose levels were not measured. 

Regurgitation was observed within one hour of dosing in all treatment groups. Because the birds regurgitated 

following dosing, actual exposure to the test compound is uncertain. Therefore, the true LD50 is likely lower than 

the value - initially calculated by the study authors. Due to the resulting uncertainty regarding actual exposure 

levels the study endpoint, this study deficiency affects the scientific soundness and acceptability of this study. 

In-life study dates were December 5-19, 2012.

G. CONCLUSIONS:

This study is classified as supplemental due to observed regurgitation, which increases uncertainty in actual 

exposure levels and the resulting LD50 estimate. Treatment-related mortality of 10, 10, 10, 10, and 80% was

observed at 125, 250, 500, 1000, and 2000 mg/kg bw groups, respectively. Signs of toxicity were observed in 

all treatment groups and included ruffled feathers, underactivity, incoordination, recumbency, body tremors, 

panting, salivation and/or regurgitation. Regurgitation was observed within one hour of dosing for 1, 1, 7, 7, 
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 11 of 13

and 6 birds in the 125, 250, 500, 1000, and 2000 mg/kg groups, respectively. There were no apparent 

treatment-related effects upon body weight or body weight change at any interval for males or females at any 

treatment group compared to the control; however, based on regurgitation observed in each of the treatment 

groups, the NOAEL is less than the lowest treatment level (< 125 mg/kg bw). The reviewer calculated the acute 

oral LD50 as 397 mg/kg with 95% confidence interval of 268 to 588 mg/kg.

LD50: 397 mg/kg bw 95% C.I.: 268 to 588 mg/kg bw

Endpoint(s) affected: Mortality and signs of toxicity (including regurgitation)

III. REFERENCES:

None; other than standard guidelines or methodologies.
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 12 of 13

APPENDIX I. OUTPUT OF REVIEWER’S STATISTICAL VERIFICATION: Calculation of % weight gain and 

comparison of % weight gain between the control and treated groups using Microsoft Excel

Male Female

Dose Day 0 Day 14 Change Day 0 Day 14 Change

0 16.8 17.9 1.1 13.3 14.1 0.8

13.3 13.9 0.6 15.1 16.1 1

17.1 17.4 0.3 13.3 14.8 1.5

15.4 16.9 1.5 13.8 15.4 1.6

15.2 15.6 0.4 16.6 18.3 1.7

Mean 15.6 16.3 0.78 14.4 15.7 1.32

St. Dev. 1.3544 1.440278 0.453431 1.273421 1.440278 0.354401

St. Error 0.605706 0.644112 0.20 0.569491 0.644112 0.16

125 17.5 18.4 0.9 14.8 17.4 2.6

17 18.1 1.1 14.1 14.9 0.8

15.4 17.1 1.7 15.1 16.2 1.1

13.1 15.5 15.8 0.3

17.5 18.6 1.1 12.2 12.1 -0.1

Mean 16.1 18.1 1.20 14.3 15.3 0.94

St. Dev. 1.686416 0.576628 0.3 1.163787 1.78146 0.926499

St. Error 0.754188 0.288314 0.15 0.520461 0.796693 0.41

250 12.7 12.9 0.2 12.8 13.7 0.9

16.2 17.5 1.3 12.4 13.4 1

14.1 15.2 1.1 13.1 14.1 1

12.8 13.6 0.8 17

15.1 16.2 1.1 22.7 24.7 2

Mean 14.2 15.1 0.90 15.6 16.5 1.23

St. Dev. 1.343726 1.677379 0.384708 3.916631 4.755195 0.449305

St. Error 0.600933 0.750147 0.17 1.751571 2.377597 0.22

500 13.6 14.5 0.9 16.8 17.5 0.7

25.9 24.5 -1.4 13.6 13.7 0.1
Data Evaluation Record on the Acute Oral Toxicity of Tribufos to Zebra Finch 

(Taeniopygia guttata)

 EPA MRID Number 49121302

Page 13 of 13

20.9 20.6 -0.3 14.1 14.3 0.2

19.8 19.6 -0.2 15.9 16.1 0.2

19.7 19.4 20.8 1.4

Mean 20.0 19.8 -0.25 16.0 16.5 0.52

St. Dev. 3.916835 3.56581 0.813941 2.077113 2.542754 0.487442

St. Error 1.751662 1.782905 0.41 0.928913 1.137154 0.22

1000 14.9 15.4 0.5 20.8 21.7 0.9

19.3 16.9 -2.4 14.1

14.5 15.2 0.7 16.4 20.3 3.9

13.1 14.9 1.8 12.9 14.3 1.4

13.7 13.8 0.1 16.1 16.4 0.3

Mean 15.1 15.2 0.14 16.1 18.2 1.63

St. Dev. 2.19089 0.997196 1.389388 3.017118 3.420892 1.581929

St. Error 0.979796 0.44596 0.62 1.349296 1.710446 0.791

2000 16.1 14.9

16.7 22.9 22.1 -0.8

16.7 15.6 -1.1 16

18.3 19.6

18.5 16.5

Mean 17.3 15.6 -1.1 18.0 22.1 -0.80

St. Dev. 0.958332 0 0 2.913005 0 0

St. Error 0.428579 0 0.000 1.302736 0 0.00
CETIS Summary Report Report Date: 14 Nov-13 08:52 (p 1 of 1)

Test Code: 074801 49121302 | 04-3500-2085

OCSPP 850.2100 Acute Avian Oral Toxicity Huntingdon Life Sciences

Batch ID: 07-6221-9965

Start Date: 05 Dec-12

Ending Date:

Test Type: Acute Avian Oral Toxicity

Duration: NA

Protocol: OCSPP 850.2100 Acute Bird Diluent: Corn Oil

Species: Taeniopygia guttata Brine: Not Applicable

Source: Lab In-House Culture

Analyst:

Age: <10m

Sample ID: 01-2835-0091

Sample Date: 05 Dec-12

Receive Date:

Code: 49121302

Sample Age: NA

Source: AMVAC Chemical Corporation

Station:

Client: CDM Smith

Material: Tribuphos Project:

PC Code 074801, MRID 49121302

PC Code 074801 MRID 49121302

Batch Note:

Sample Note:

95% LCL 95% UCL

Point Estimate Summary

Analysis ID Endpoint Level mg ai/kgB TU Method

15-6993-1879 14dMortalityRate LC5 1070 N/A N/A Linear Regression (MLE)

LC10 1180 N/A N/A

LC15 1260 N/A N/A

LC20 1320 N/A N/A

LC25 1380 N/A N/A

LC40 1530 N/A N/A

LC50 1640 N/A N/A

17-1363-5181 14dMortalityRate LC50 1490 1260 1750 Trimmed Spearman-Kärber

C-mg ai/kgB Control Type Count Mean Min Max Std Err Std Dev CV%

14dMortalityRate Summary

95% LCL 95% UCL %Effect

0 Negative Control 1 0 0 0 0 0

125 1 0.1 0.1 0.1 0 0 0.0%

250 1 0.1 0.1 0.1 0 0 0.0%

500 1 0.1 0.1 0.1 0 0 0.0%

1000 1 0.1 0.1 0.1 0 0 0.0%

2000 1 0.8 0.8 0.8 0 0 0.0%

000-516-187-1 CETISTM v1.8.7.12 Analyst:________ QA:________
CETIS Summary Report Report Date: 14 Nov-13 08:52 (p 1 of 1)

Test Code: 074801 49121302 | 04-3500-2085

OCSPP 850.2100 Acute Avian Oral Toxicity Huntingdon Life Sciences

Batch ID: 07-6221-9965

Start Date: 05 Dec-12

Ending Date:

Test Type: Acute Avian Oral Toxicity

Duration: NA

Protocol: OCSPP 850.2100 Acute Bird Diluent: Corn Oil

Species: Taeniopygia guttata Brine: Not Applicable

Source: Lab In-House Culture

Analyst:

Age: <10m

Sample ID: 01-2835-0091

Sample Date: 05 Dec-12

Receive Date:

Code: 49121302

Sample Age: NA

Source: AMVAC Chemical Corporation

Station:

Client: CDM Smith

Material: Tribuphos Project:

PC Code 074801, MRID 49121302

PC Code 074801 MRID 49121302

Batch Note:

Sample Note:

95% LCL 95% UCL

Point Estimate Summary

Analysis ID Endpoint Level mg ai/kgB TU Method

15-6993-1879 14dMortalityRate LC5 1070 N/A N/A Linear Regression (MLE)

LC10 1180 N/A N/A

LC15 1260 N/A N/A

LC20 1320 N/A N/A

LC25 1380 N/A N/A

LC40 1530 N/A N/A

LC50 1640 N/A N/A

17-1363-5181 14dMortalityRate LC50 1490 1260 1750 Trimmed Spearman-Kärber

C-mg ai/kgB Control Type Count Mean Min Max Std Err Std Dev CV%

14dMortalityRate Summary

95% LCL 95% UCL %Effect

0 Negative Control 1 0 0 0 0 0

125 1 0.1 0.1 0.1 0 0 0.0%

250 1 0.1 0.1 0.1 0 0 0.0%

500 1 0.1 0.1 0.1 0 0 0.0%

1000 1 0.1 0.1 0.1 0 0 0.0%

2000 1 0.8 0.8 0.8 0 0 0.0%

000-516-187-1 CETISTM v1.8.7.12 Analyst:________ QA:________