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                            Funding & Awards
 Plant DNA Typing by AFLP
Award Information
Awardee
              University of New Haven - School of Public Safety
Award #
              2001-IJ-CX-K011
Funding Category
              Continuation
Location
                    West Haven, CT
Awardee County
              New Haven
Congressional District
              3
Status
              Closed
Funding First Awarded
              2001
Total funding (to date)
              $259,868
Original Solicitation
              National Institute of Justice Continuation Awards
Description of original award (Fiscal Year 2001, $259,868)
PROJECT SUMMARY FOR 2001-IJ-CX-K011

This project will be performed by the University of New Haven's Institute of Forensic Science in collaboration with the Connecticut State Forensic Science Laboratory.  The research will focus on a single plant species - Cannabis sativa - for the development of a molecular strategy for plant DNA typing.  Cannabis (marijuana) was selected because it is an illegal substance grown across the United States and in other countries.  The Amplified Fragment Length Polymorphism (AFLP) technology will initially be validated for forensic applications.  Preliminary studies performed by the grantee have already shown that marijuana plants known to have originated from the same mother plant have identical and reproductible AFLP DNA profiles.  Completion of the validation studies will include interlaboratory reproductibility studies, DNA degradation studies, and a survey of marijuana samples from Connecticut and other geographical regions.  This database will be used to determine the extent of genetic diversity in marijuana populations and will consist of a minimum of 500 individual samples.  This database will additionally allow the estimation of the probability of a random match between two marijuana samples.  It is possible that AFLP patterns will group marijuana samples that are genetically related to a similar geographic location.  This in turn would aid in tracking distribution networks of this illegal substance.  The final phase of this project includes sponsoring plant typing workshops to train other members of the forensic community in the AFLP technique.


ca/ncf



  
Grant-Funded Publications
        
                      Evaluation of plant seed DNA and botanical evidence for potential forensic applications
                  Taking the Initiative: Practitioners Who Perform Frontline Research
                                                                      NIJ Journal,
                  Validation of a DNA Method for the Individualization of Plant Evidence
                  Overview of DNA Methods for the Identification and Individualization of Marijuana

  
Date Created: September 29, 2001
    
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