11II1111111111111111111111111111 11111111 SANITIZED SUBMISSION 

PMN2010P1 	 PMN Page 1 

,.---------_. Form Approved. O.M.B. Nos. 2070-0012 and 2070·0038 

! u.s. ENVIRONMENTAL PROTECTION AGENCY -/0 AGENCY USE ONLY 

P~r::MAt.JIII:A("TllgC Date of receipt: 

Ep, 111111111111111111111111111 111111111111111 111111111111111111111111111111111 51110000128 P·11·01

51110000 1 281$ 

Submission ....onl"\'" Number 

K944HX 

must I 	 this form to the extent that it IS known i by you. Make reasonable estimates If you 00 not haye actual data. 

• 	 Before you complete this form. you should read the "Instructions Manual for Premanufacture Notification" (the Instructions Manual is available from the Toxic Substances Control Act 

(,:,SCA) Information Sef'llce by calling 202-554-1404. or faxing 202-554-5603) 

If a user fee has been remitted for this nollce (40 CFR 700.45). indicate in Ihe boxes aboye Ihe TS-user fee ident~lca!ion number you have generated Remember. your user fee ID number 

must also appear on your corresponding fee remittance For mailing address information see the Help instructions In the e-PMN tool. 

Part 1- GENERAL INFORMATION 

You must provide the currently correct Chemical Abstracts (CA) 

Name of the new chemical substance, even if you claim the 

identity as confidentiaL You may authorize another person to 

submit chemical identity information for you, but your submission 

will not be complete and the review will not begin until EPA 

receives this information. A letter in support of your submission 

should reference your TS user fee identification number. For all 

Section 5 Notice submissions (paper or electronic) you must 

submit an original notice including all test data; if you claimed any 

information as confidential. an original sanitized copy must also be 

submitted. 

Part 11- HUMAN EXPOSURE AND ENVIRONMENTAL 

RELEASE 

If there are several manufacture, processing, or use operations to 

be d"scribed in Part II, sections A and B of this notice, reproduce 

the sections as needed. 

Part 111- LIST OF ATTACHMENTS 

For paper submissions. attach additional sheets if there is not 

enough space to answer a question fully. Label each continuation 

sheet with tlhe corrt!sponding section heading. In Part III, list these 

attachments, any test data or other data and any optional 

information included in the notice. 

OPTIONAL INFORMATION 

You may include any information that you want EPA to conSider in 

evaluating the new substance. On page 11 of this form, space has 

been provided for you to describe pollution prevention and 

recycling information you may have reg:;!rd ing the new substance. 

"Binding" boxes are included throughout this form for you to 

indicate your willingness to be bound to cenain statements you 

make in this section, such as use. production volume, protective 

equipment. . The intention is to reduce delays tlhat routinely 

accompany the development of consent orders or :>ignificant New 

Use Rules. Checking a "binding" box in a PMN does not by itself 

prohibit the submitter from later deviatIng from the infor,nation 

(except chemical identity) reported in the form; however, In tlhe 

case of exemption applications (such as TMEA, LVE, LOREX) 

certain information provided in such notifications IS binding on :~e 

submitter when the Agency approves the exemption application, 

especially if the production volume "binding" box is chosen in a 

LVE. 

CONFIDENTIALITY CLAIMS 

You may claim any information In this notice as confidential. To 

assert a claim on the form, mark (X) the confidential box next to 

the Information that you claim as confidentiaL To assert a claim in 

an attachment circle or bracket the information you claim as 

confidential. If you claim information in the notices as confidential 

you must also provide a sanitized version of the notice (including 

attachments) For additional instructions on claiming information 

as confidential. read the Instructions Manual. 

TEST DATA AND OTHER DATA 

You are required to submit all test data in your possession or control and to provide a 

description of all other data known to or reasonably ascertainable by you. if these data are 

related to the health and environmental effects on the manufacture, processing. distribution in 

commerce, use, or disposal of the new chemical SUbstance. Standard literature citations may 

be submitted for data in the open scientific literature. Complete test data (wrillen in English) 

not summaries of data, must be submitted if they do not appear in the open literature. You 

should clearly identify whether test data is on the substance or on an analog. Also. tlhe 

chemical composition of the tested material shOuld be characterized. Following are examples 

of test data and other data. Data should be submitted according to the requirements of 

§720.50 of the Premanufacture Notnication Rule (40 CFR Part 720). 

Test Data (Check Below any included in this notice) 

Environmental fate data 	 W Other Data 

Health effects data 	 D Risk Assessments 

o 	EnVIronmental effects data 0 Structure/activity relationships 

Physical/Chemical Properties (A physical and chemical properties worksheet is o located on the last page of this form.) 

D Test data not in the possession or control mthe submitter 

TYPE OF NOTICE (Check Only One) 

PMN (Premanufacture Notice) 

D SNUN (Significant New Use Notice) 

TMEA (Test Marketing Exemption Application) D 	 '.,~

. D LVE (Low Volume Exemption)@40CFR 723.50(c)(1) 

D LOREX (Low Release/Low Exposure Exemption)@ 40 CFR 723.50(c)(2) 

D L VE Modification 

D LOREX Modification 

Mock Submission 

Mark (X) if pending Letter of Support 

:S THIS A CONSOLIDATED PMN (Y/N)? 

/I of chemicals or polymers (Prenotice Communication # required. enter # on 

p. J) 

information in this notice is claimed as confidential. 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 1 P·II-OU8 
SANITIZED SUBMISSION 1111111111111111111111111111111111111111111111111111111 

PMN2010P2 PMN Page 2 

Include the OMB 

The public reporting and record keeping burden for this collection of information is estimated to average 93 hours pe

comments on the Agency's need for this information, the accuracy of the provided burden estimates, and any sugg

minimizing respondent burden, including through the use of automated collection techniques to the Director, Collect

Division, U.S. Environmental Protection Agency (2822T), 1200 Pennsylvania Ave., NW, Washington, D.C. 20460. 

control number in any correspondence. Do not send the completed EPA Form 7710-25 to this address. 

CERTIFICATION -- A printed copy of this signature page, with original signature, must be sub

with CD or paper submission. 

ested methods for 

r response. Send 

ion Strategies 

mitted 

I certify that to the best of my knowledge and belief: 

1. The company named in Part I, section A, subsection 1a of this notice form intends to 

manufacture, import or process for a commercial purpose, other than in small quantities sole

research and development, the substance identified in Part I, Section B. 

2. All information provided in this notice is complete and truthful as of the date of submission. 

3. 1am submitting with this notice all test data in my possession or control and a description of 

other data known to or reasonably ascertainable by me as required by §720.50 of the 

Premanufacture Notification Rule. 

Additional Certification Statements: 

If you are submitting a PMN, Intermediate PMN, Consolidated PMN, or SNUN, check the following user fee 

ly for 

all 

• certification statement that applies: 

f--------------------------------------..--..--..-----j 

[Xl The Company named in Part I, Section A has remitted the fee of $2500 specified in 40 CFR 700.45(b), or 

The Company named in Part I, Section A has remitted the fee of $1000 for an Intermediate PMN (defined @ 40 CFR 700.43) in 

accordance with 40 CFR 700.45(b), or 

The Company named in Part I Section A is a small business concem under 40 CFR 700.43 and has remitted a fee of $100 in o accordance with 40 CFR 700.45(b). 

If you are submitting a Low Volume Exemption (LVE) application in accordance with 40 CFR 723.50(c)(1) or a 

Low Release and Low Exposure Exemption (LoRex) application in accordance with 40 CFR 723.50(c)(2), check 

the following certification statements: 

The manufacturer submitting this notice intends to manufacture or import the new chemical substance for commercial purposes, o other than in small quantities solely for research and development, under the terms of 40 CFR 723.50. 

The manufacturer is familiar with the terms of this section and will comply with those terms: and 

o The new chemical substance for which the notice is submitted meets all applicable exemption conditions. 

If this application is for an LVE in accordance with 40 CFR 723.50(c)(1), the manufacturer intends to commence manufacture of o the exempted substance for commercial purposes within 1 year of the date of the expiration of the 30 day review period. 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 2 

SANITIZED SUBMISSION 111111111 11111 11111 III 111111111111111 1111111 III I11I 1111 

PMN2010P3 

D 

D 

Mailing Address (number & street) 


City Postal Code 


(last) Van Norstrand 

D 

Peveler 

Position 

Company Eastman Kodak 

Mailing Address (number & street) 

City Rochester 

e-mail judith.vannorstrand@kodak.com 

3. 

If you have had a prenotice communication (PC

this notice and EPA assigned a PC Number to 

4. 

enter the number. 

the notice, 

) concerning 

the xxx 

If you have submitted a notice of Bona fide intent to 

5. 	 manufacture or import for the chemical substance covered 

by this notice, enter the notice number assigned by EPA. D 

Manufacture Only D 	 1. i 3. Both

Binding Option D 

EPA FORM 7710-25 (Rev. 6-09) 	 Replaces previous editions of EPA Form 7710-25 

Page 3 
SANITIZED SUBMISSION 1111111111111111111111111111111111111111111111111111111 

You must provide a currently correct Chemical MlJ:>"<''''l> 

based on current CA index nomenclature ,..n,w"nli/,n<:. 

Mark (X) the "Confidential" box next to any item you claim as confidential 

r-··· 

. Complete either item 1 (Class 1 or 2 substances) or 2 (Polymers) as appropriate. Complete all other items. 

If another person will submit chemical identity information for you (for either Item 1 or 2), mark (X) the box at the right. Identify D I 


the name, com an ,and address of that erson in a continuation sheet. 


1. 	 Class 1 or 2 chemical substances (for definitions of class 1 and class Class 1 Class 2 


2 substances, see the Instructions Manual) 


a. 	 Class of substance - Mark (X) D D 

b. Chemical name (Currently correct Chemical Abstracts (CA) Name that is consistent with TSCA Inventory listings for similar 

substances. For Class 1 substances a CA Index Name must be provided. For Class 2 substances either a CA Index Name or CA 

Preferred Name must bEl providEld, which ever is appropriate based on current CA index nomenclature rules and conventions). 

3H-lndolium, 2-[2-r3-[2-(1 ,3-dihydro-1 ,3,3-trimethyl-2H-indol-2-ylidene)ethylidene]-2-[1-(phenyl-1 Htetrazol-5-yl)thio]-f -cyclohexen-1-yl]ethenyl]-1 ,3,3-trimethyl-. cflloride 

D 

I 

CAS Registry Number (if a number already exists for the substance) 440102-72-7 I 

c. Please identiJ}' which method you used to devE!lop or obtain the specified chemical identity information reported in this notice: (check one). 

Method 1 (CAS Inventory Expert Service - a copy of the [X] IES Order I Method 2 D I 

Identification report obtained from the CAS Inventory Expert Number ' (Other 

~rvices must be submitted as an attachment to this notice) 102995-2 , Source) I

Enter Attachment filename for Part I, Section B, 1. c. D 

d. Molecular formula IC39 H41 N6 S . CI D e. For a class 1 substance. provide a complete and correct chemical structure diagram. For a class 2 substance, provide a correct 

representa!ive or partial chemical structure diagram, as complete as canbe known, if one can be reasonably ascertained. D

I 

i 

i 

EPA FORM 7710-25 (Rev. 6-09) 	 Replaces previOUS editions of EPA Form 7710-25 

Page 4 
SANITIZED SUBMISSION 1111111111111111111111111111111111111111111111111111111II111 

PMN2010P4A _________~P_M_N_Page 4a i 

For a class 2 substance (1) list the immediate precursor substances with their respective CAS Registry Numbers. (2) Describe 

. the nature of the reaction or process. (3) Indicate the range of composition and the typical composition (where appropriate). Confidential I __.____'---___-1 

e. (1) list the immediate precursor substance names with their respective CAS Registry Numbers. 

Enter Attachment filename for Part I. Section B. 1. e. (1) I [ ] e. (2) Describe the nature of the reaction or process. D 

Enter Attachment filename for Part I. Section B. 1. e. (2) I D e. (3) Indicate the range of composition and the typical composition (where appropriate). D 

Attachment filename for Part I. Section B. 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 5 
SANITIZED SUBMISSION 1111111111111111111111111111111111111111111111111111111 

PMN2010P5 	 PMN Pag;;;t.!l-=-::5___ ____ _____~_---, 

·---P-a-rt-I----G~E=N~E=RA=--:c-L INFORMATION - Continu=-=e-=d_______~ 

Section B -- CHEMICAL IDENTITY INFORMATION -- Continued 

2. Eolymerl) (For a definition of polymer, see the Instructions Manual.) . 	 ..___•.__... I Confidential 

a. 	

Indicate 

Indicate 

maximum 

the number-average 

weight percent 

weight 

of low 

of the 

mOlec.ular 

lowest molecular 

weight species 

weight 

(not 

composition 

including 

of 

residua. 

the polymer 

I monomers, 

you intend 

reactants, 

to manufacture. 

or solvents) 

~. 

I 0 i below 500 and below 1,000 absolute molecular weig ht of that composition. . ..._______ . 

Describe the methods of measurement or the basis for your estimates: 

t-G-PC------=O==---rl-O-t-he-r-(-SP-e- cify Below) 0 I ---------..

Specify Other: 

(i) lowest number average molecular 

I 

(ii) maximum weight % below 500 molecular (iii) maximum weight % below 1000 molecular I 

weight: weight: weight: i 

I 

! 

Enter Attachment filename for Part I, Section B, 2. a. I [ I 

b. You must make separate confidentiality claims for monomer or other reactant identity, composition information, and residual information. Mark 

(X) the "Confidential" box next to any item you claim as confidential 

(1) Provide the specific chemical name and CAS Registry Number (if a number exists) of each monomer or other reactant used in the 

manufacture of the polymer. 

(2) - Mark (X) this column if entry in column (1) is confidential. 

(3) - Indicate the typical weight percent of each monomer or other reactant in the polymer. 

(4) - Choose "yes" from drop down menu if you want a monomer or other reactant used at two weight percent or less to be listed as part of 

the polymer description on the TSCA Chemical Substance Inventory. 

(5) - Mark (X) this column if entries in columns (3) and (4) are confidential. 

I 

(6) Indicate the maximum weight percent of each monomer or other reactant that may be present as a residual in the polymer as 

manufactured for commercial purposes. 

(7) - Mark (X) this column if entry in column(6} is confidential. 

Monomer or other reactant specific chemical name I Typical Include in Max 

CBI composition identity CBI residual CBI 

(2) '(3) (4) (5) (6) _(7) 

I 

CAS Registry Number (1) I I I 

I I I 

CAS Registry Number (1) I i I 

1 1 

CAS Registry Number (1) I 

CAS Registry Number (1) 

the next page. 

EPA FORM 7710-25 (Rev, 6-09) Replaces previous editions of EPA Form 7710-25 

Page 6 
SANITIZED SUBMISSION 

PMN2010P5A 

111111111111111111111111111111111111I111111111I11~1I11111 II PMNPag~e~5_a~~~~~.__~~~~______--, i c. Please identify which method you used to develop or obtain the specified chemical identity information reported in this notice I· CBI (check one). 


Method 1 (CAS Inventory Expert Service I I I .. I 
 • a copy of the identification report obtained 0 IES Order I Method 2 D· 


from CAS Inventory Expert Service must be . Number (other source) 
 I ~ubmitted as an attachment to this notice) L-__________L._-;===;---', 

. Enter Attachment filename for Part I, Section B, 2. c. I 0 

I d. The currently correct Chemical Abstracts (CAl name for the polymer that is consistent with TSCA Inventory listings for similar 

",VIY" ''''',. o 

I CAS Registry Number (if a number already exists for the substance) I I ! e. Provide a correct representative or partial chemical structure diagram, as complete as can be known, if one can be reasonably 

ascertained. o 

EPA FORM 7710·25 (Rev. 6·09) Replaces previous editions of EPA Form 7710·25 

Page 7 
-------------------------------

1111111111111111111111111111111111111111111111111111111 SANITIZED SUBMISSION

PMN2010P6 	 PMN Page 6 -~-P-a-rt-'I-----=:G-=E-:-:N=E~=RA-::-::-L~IN~FO=-=R~M~A--:::T;:-:IO::-:N:-;-----::C=-o-n~ti-;-n-u-ed-;;- r--------------------~~--~=

Section B -- CHEMICAL IDENTITY INFORMATION -- Continued 

3. Impurities 

(a) 

(b) 

- Identify each impurity that may be reasonably anticipated to be present in the chemical substance as manufactured for commercial 

purpose. Provide the CAS Registry Number if available. If there are unidentified impurities, enter "unidentified." 

Estimate the maximum weight % of each impurity. If there are unidentified impurities, estimate their total weight %. ...-;---,-------1 

. 

Impurity (a) 

i CAS Registry I Maximum 

. Number Percent % 

b 

xxx 

xxx xxx 

I 

Mark (X) this box if the data continues on the next page. 0 '---. .----_. 

Enter Attachment filename for Part I, Section B, 3. I D

4. Synonyms - Enter any chemical synonyms for the new chemical identified in subsection 1 or 2. 

None D 

Enter Attachment filename for Part I, Section B, 4. I 	 D 

5. Trade identification - List trade names for the new chemical substance identified in subsection 1 or 2. 


S 0507 I 


Cyanine 

ORM1835 	

dye 


i D

I 

Enter Attachment filename for Part I, Section B, 5. I D 

6. 	 Generic chemical name - If you claim chemical identify as confidential, you must provide a generic name for your substance that reveals the 

specific chemical identity of the new chemical substance to the maximum extent possible. Refer to the TSCA Chemical 

Substance Inventory, 1985 Edition, Appendix B for guidance on developing generic names. 

Substituted aromatic tetrazol halide 

Enter Attachment filename for Part I, Section B, 6. 	 , I 

7. 	 Byproducts - Describe any byproducts resulting from the manufacture, processing, use, or disposal of the new chemical substance. Provide the 

CAS Registry Number if available. 

CAS Registry Number Confi­

_(2) dential 

103-84-4 

64-19-7 

'"'''''''''' ""C. fI.-flh,,, ,yl· 

I 

i 

(X) data continues on the next page. I ] 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 8 
SANITIZED SUBMISSION I1I1I1111111111111111111111111111111111111111111111111I 

PMN2010P7 	 PMN Page 7 

Part 1-- GENERAL INFORMATION -- Continued 

Section C -- PRODUCTION, IMPORT, AND USE INFORMATION: 

The information o~ this page refers to consolidated chemical number(s): 0 1 m 0 2 0 30.~~~~~~--~~----- 4 0 5 0 6 

Mark (Xl the "Confidential" box next to any item you claim as confidential. 

1. 	 Produc-t-io-n-v-o-'-Iu-m·-e---~E-s-tim-at-e:c.;the maximum production volume during the first 12 months of production. Also estimate the maximum production 

volume for any consecutive 12-month period during the first three years of production. Estimates should be on 100% new chemical substance basis. 

For a Low Volume Exemption application, if you choose to have your notice reviewed at a lower production VOlume than 10,000 kg/yr, specify the 

volume and mark (x) in the binding box. If granted, you are bound to this volume. 

Maximum first 12-month production (kg/yr) I Maximum 12-month production (kg/yr) Confidential Binding Option I· 

(100% new chemical substance basis) • (100% new chemical substance basis) 	 Mark (X) 

--~--~-~--~--O--~I 

ixxx 	 xxx ~.------------------------------------~------~------------------------ ~--------~----- ~==,---~ Enter Attachment filename for Part I, Section C, 1. 	 CBI o 

2. 	 Use Information -- You must make separate confidentiality claims for the description of the category of use, the percent of production volume devoted 

to each category, the formulation of the new substance, and other use information. Mark (X) the "Confidential" Box next to any item you claim as 

confidential. 

a. 	 (1) --Describe each intended category of use of the new chemical substance by function and application. 

(2) --Mark (X) this column if entry column (1) is confidential business information (CBI). 

(3) --Indicate your willingness to have the information provided in column (1) binding. 

(4) --Estimate the percent of total production for the first three years devoted to each category of use. 

(5) --Mark (X) this column if entry in column (4) is confidential business information (CBI). 

(6) --Estimate the percent of the new substance as formulated in mixtures, suspensions, emulsions, solutions, or gels as manufactured for 

commercial purposes at sites under your control associated with each category of use. 

(7) --Mark (X) this column if entry in column (6) is confidential business information (CBI). 

(8) --Indicate % of product volume expected for the listed "use" sectors. Mark more than one box if appropriate. Mark (X) to indicate your 

willingness to have the use type provided in (8) binding. 

(9) --MarklX) this column if entrv(ies) in column (8) is (are) confidential business information (CB!). 

I 

Category of use (1) Binding Prod ' % in 

I

• (by function and application Le. a dispersive dye for I i· CBI Option uction CBI Form- I finishing polyester fibers) 	 ! Mark (X) • % ulation 

(2) (3) (4) (5) (6) 

XXX 

XXX x XXX 

CBI 

(7) 

x 

x 

i 	 I 

l 	 I

1 T 

% of substance expected per use Ii I, 

(8) CBI 

Si~e. Com­ Bioding ! I Con-	 ! Industrial 1imited i $Umer~ rner<:ial Option (9) 

XXX IXXX TXXX XXX I x 

i 

XXX XXX XXX IXXX X i 

i 

I 	 i 

i 

I i I I t-----.---..------·----+-----r----+--.J--,----,----I--+---+I---+---...L-+---I 

I 	 I !

~___:__:_:_~_o",,--:-----":-:-i--l...-....I . : 	 i ... I I I . I 

* If you have identified a "consumer" use, please provide on a continuation sheet a detailed description of the use(s) of this chemical substance in 

consumer products. In addition include estimates of the concentration of the new chemical substance as expected in consumer products and describe I

• the chemical reactions by which this substance loses its identity in the consumer product. 

i Mark (X) this box if the data continues on the next page. o 

b. Generic use If you claim any category of use description in subsection 2a as confidential, enter a generic description of that category. I description Read the Instruction Manual for examples of generic use descriptions. 

i Contained use in an article 

I Export. 

Enter Attachment filename for Part I, Section C, 2. b. I 	 I CBI D 

3. 	Hazard Information -- Include in the notice a copy of reasonable facsimile of any hazard warning statement, label, material safety 


data sheet, or other information which will be provided to any person who is reasonably likely to be exposed to this substance 
 Binding Option 

regarding protective eqUipment or practices for the safe handing, transport, use, or disposal of the new substance. List in part III Mark (X) 

hazard information you include. i 

Mark (X) this box if you attach hazard information. 1X1 D 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 9 

i 

i 
SANITIZED SUBMISSION 11111111111111111111111111111111 11111111111 

PMN2010P8 	 PMN Page 8 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

. Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER 

The information on pages 8 and 8a refer to consolidated chemical number(s): 1 D 2 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites 

• you control Importers do not have to complete this section for operations outside the US' ., however , you may still have reporting

i 

requirements if there are further industrial processing or use operations after import. You must describe these operations. See 

instructions manual 

1. Operation description Confia.ldentity -­ Enter the identity of the site at which the operation will occur. dential 

Name IEastman Business Park 

Site address (number and 

street) 11669 Lake Avenue D 

City IRochester County I Monroe 

State INY ZIP code 114652 

If the same operation will occur at more than one site, enter the number of sites. Identify the additional 

sites on a continuation sheet, and if any of the sites have significantly different production rates or 

I 

1 

-pperations, include all the information reguested in this section for those sites as attachments. ~ 

Mark (X) this box if the data continues on the next page. D 

b. Type-­ Manufacturing D Processing D Use D [K] Mark (X) 

c. Amount and Duration Complete 1 or 2 as appropriate Confidential 

Maximum kg/batch 

(100% new chemical Hours/batch Batches/year 

1. Batch substance) [K]

XXX XXX XXX 

Maximum kg/day Hours/day Days/year (100% new chemical substance) D 

Mark (X) to indicate your willingness to 

d. Process description have 

~ 

your process description binding. D

I 

I 

(1 ) Diagram the major unit operation steps and chemical conversions. Include interim storage ancitransport containers (specify- e.g. 5 gallon i palls, 55 gallon drum, rail car, tank truck, etc.). 

(2) 	 Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting 

materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning 

chemicals (note frequency if not used daily or per batch.). 

(3) 	 Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If 

releasin to tW0rTledia at the same step, assign a second release number for the second medium ... -----------;==.---I 

[Xl 

XXX 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 10 
IIIIIIIIIIIIIIIIIIIIIIIIIIIII~IIIIIIIIIIIIIIIIIIIIIIIIII III SANITIZED SUBMISSION 

PMN2010P8A PMN Page 8a 

Diagram of the major unit operation steps. 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 11 

SANITIZED SUBMISSION 1111111111111111111111111111111111111111111111111111111 

PMN2010P9 	 PMN Pa e 9 

k=.Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE - Continued 

• Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER -- Continued :==----­

The information on pages 9 and 9a refer to consolidated chemical number(s): 0 1 0 2 0 3 0 4 ......0=,-5__=0=--_6__ 

2. Occupational Exposure -- You must make separate confidentiality claims for the description of worker activity. physical form of the new chemical 

substance, number of workers exposed, and duration of activity. Mark (X) the ·Confidential" box next to any item you claim as confidential. 

(1) 	-- Describe the activities (Le. bag dumping, tote filling. unloading drums. sampling, cleaning, etc.) in which workers may be exposed to the 

substance. 

(2) 	 Mark (X) this column if entry in column (1) is confidential business information (CSI). 

(3) --	 Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) -- Indicate your willingness to have the information provided in column (3) or (5) binding. 

(5) 	-- Indicate the physical form(s) of the new chemical substance (e.g., solid: crystal, granule. powder, or dust) and % new chemical substance (if I 

part of a mixture) at the time of exposure. 

(7) -- Mark (X) this column if entries in columns (3) and (5) are confidential business information (CSI). 	 • 

(8) --	 Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) 	 Mark (X) this column if entry in column (8) is confidential business information (CBI). 

(10) and (11 ) Estimate the maximum duration 0 f the activity for any worker in hours per day and days per year. 

(12) -­ Mark (Xl this column if entries in columns (10) and (11) are confidential business information (CBI). 

Worker activity Protective Equipment! Binding Physical Binding #of Maximum Duration (i.e., bag dumping, filling CBI Option form(s) Option CBI Workers CBI CBI 

drums) Engineering Controls Mark (X) &%new Mark (X) Exposed 

(i) (2) (3) (4) substance (6) (7) (8) (9) Hrs/Day DaysNr (12) i 

(6) (10) (11) 

Addition of seed crystal to see MSDS · Solid, 100% 1 0.04 13 

reaclor ! i 

IHandling and cleaning of 

I 

Isee MSDS • Paste, -50% 

I 

1 I 0.5 I 13 

• filter 

i i 

i i i 

Treatment of waterllPA see MSDS Liquid 1 I 0.5 i 13 

I liquors from reaction with i sodium hypochlorite i 

I I i I I 

I 

! Collection of crude see MSDS • Paste. -50% 1 i 

2 13 

, product 

i 

i 

i ---­ i Addition of damp crude i see MSDS Paste, -50% i 1 1 0.5 13 i product to reactor 

I i I I i i i I I I i , 'see I 

I I ! i 

; to trays i 

Transfer to dried product see MSDS Solid,100% i 13 

from trays to grinder 

Grinding of final product see MSDS Solid,100% 13 

I Packaging of ground see MSDS Solid, 100% 0.5 13 

product 

Attachment filename for Part II, Section A on the bottom of page 9a. 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 12 
SANITIZED SUBMISSION 111111111111111111111111111111111111111111111111111111111111 

PMN2010P9A PMN Paae Sa 

3. Environmental Release and Disposal You must make separate confidentiality claims for the release number and the amount of the new chemical 

substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(1) -­ Enter the number of each release point identified in the process description. part II. section A, subsection ld(3). 

(2) Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) -­ Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). 

(4) -­ Identify the media (stack air. fugitive air (optional-see Instruction Manual). surface water. on-site or off-site land or incineration. POTW. or 

other (specify» to which the new substance will be released from that release pOint. 

(5) -­ a. Describe control technology. if any. and control efficiency that will be used to limit the release of the new substance to the environment. 

For releases disposed of on land. characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. 

On a continuation sheet. for each site describe any additional disposal methods that will be used and whether the waste is subject to 

secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). 

(6) Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct 

discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES # is confidential 

business information (CBI). 

Release Amount of New Medium of release Control technology and efficiency (you may wish to 

Number Substance Released CBI e.g. Stack air optionally attach efficiency data) CBI 

Binding 

(1 ) (2a) (2b) (3) (4) (Sa) Mark (5b) (6) I (Xl 

! 

I 

i 

1 0.05 : Other: Landfill 

I 

I 0.1 Other: Landfill i 

i I 

I < 1 kg Other: Wastewater treatment See continuation page. id: 

I 

<0.1 kg i 

I I 

plant : <P9ASA3( 5a)C 1 R3> 

I i 

4 < 1 kg Other: Wastewater treatment See continuation page. id: 

I 

< 0.1 kg 

plant <P9ASA3(5a)C1 R4> 

i 

5 < 1 kg 

I 

IOther: Landfill I ! 

6 Iincineratio~

I I 

< 10 kg . Off-site Incineration 0 

7 

I 

< 1 kg Other: Landfill 

I I 

8 i < 2 kg Other: Wastewater treatment See continuation page. id: < 2 kg 

I 

plant <P9ASA3(5a)Cl R8> 

I 

9 IOff-,'to I"'c."boo Incineration 0 i 

I I I I ! Mark (X) this box if the data continues on the next page. [R] 

i (7) Mark (X) the destination(s of releases to water. I NPDES# CBI 

! i

0 POTW·-provide 

name(s) i

I i I 

I Genesee River after wastewater treatment plant : 

[R] I Navigable waterway- NY -000-1643 

- provide name(s) 0

I 

I 0 

Enter Attachment filename for Part II, Section A. I 0 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 13 
SANITIZED SUBMISSION 11111111111111111111111111111111 11111111111111111111111111 

PMN2010P9A-1 

3. Environmental Release and Disposal - You must make separate confidentiality claims for the release number and the amount of the new chemical 

substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(1) Enter the number of each release point identified in the process description, part II, section A, subsection 1d(3). 

(2) Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) -­ Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). 

(4) Identify the media (stack air fugitive air (optional-see Instruction Manual) surface water on-site or off-site land or incineration POTW or 

other (specify)} to which the new substance will be released from that release point. 

(5) -­ a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. 

For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. 

On a continuation sheet. for each site describe any additional disposal methods that will be used and whether the waste is subject to 

secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). 

(6) Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct 

discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES # is confidential 

business information (CB!). 

Release Amount of New Medium of release Control technology and efficiency (you may wish to 

Number Substance Released CBI e.g, Stack air optionally attach efficiency data) CBI 

Binding 

(1) (2a) (2b) (3) (4) (Sa) Mark (5b) (6) 

(Xl 

1a 0.05 kg Off-site Incineration Incineration 0 

2a 0.1 kg Off-site Incineration Incineration 0 

Sa < 1 kg Off-site Incineration Incineration 0 

7a < 1 kg Off-site Incineration Incineration 0 

Mark (X) this box if the data continues on the next page. D 

(7) Mark (X) the destination(s of releases to water. NPDES# eBI 

D POTW--provide 

name(s) D

D Navigable waterwayprovide name(s) 

• 

D 

D Other--Specify D 

I 

Enter Attachment •filename for Part II. Section A. 1 D 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 14 
1111111111111111111111111111111111111111111111111111111111111111111111 SANITIZED SUBMISSION 

PMN2010P9A-2 

Continuation Sheet 

ID IP9ASA3(5a)C1R3 I Field I Part II. Section A. B.(5a) Control Technology & Efficiency. Row 3 

Reaction with sodium hypochlorite to decompose PMN substance. The treated solution is sent to an on-site wastewater treatment plant and then 

to an industrial sewer. 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 15 

11111111111111111111111111111111 11111111111111111111111111 SANITIZED SUBMISSION 

PMN2010P9A-3 

Continuation Sheet 

In IP9ASA3(5a)C1 R4 I Field I Part II, Section A, B.(5a) Control Technology & Efficiency, Row 4 

Reaction with sodium hypochlorite to decompose PMN substance. The treated solution is sent to an on-site wastewater treatment plant and then 

to an industrial sewer. 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 16 

11111111111111111111111111111111111111111111111111111111I1111111111111 SANITIZED SUBMISSION 

PMN2010P9A-4 

Continuation Sheet 

ID IP9ASA3(5a)C1R8 I Field I Part II, Section A, B.(5a) Control Technology & Efficiency, Row 8 

Aqueous streams generated from manufacturing operations or final equipment rinsing operations that may contain small amounts of the PMN 

substance will undergo on-site biological wastewater treatment, and sludge will be disposed of in compliance with applicable regulations; Treated 

process streams will be released via plant sewer lines 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 17 

SANITIZED SUBMISSION 11111111111111111111111111111111 111111111111111111111 


PMN2010P8X1 PMN P age 8 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUBMITTER I Mark (X) the "Confidential" box nex1 to 

any item you claim as confidential 

The information on pages 8 and 8a refer to consolidated chemical number(s): 01 02 03 04 Os 06 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites 

you control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting 

requirements if there are further industrial processing or use operations after import. You must describe these operations. See 

instructions manual 

1. Operation description Confia. Identity -­ Enter the identity of the site at which the operation will occur. dential 

Name Graphics Communications Group of Eastman Kodak Company 

Site address (number and 

street) One Polychrome Park, Corporate Ridge Industrial Park 0 

City Columbus County Muscogee 

State GA ZIP code 31907 

If the same operation will occur at more than one site, enter the number of sites. Identify the additional 

sites on a continuation sheet, and if any of the sites have significantly different production rates or 1 0

operations, include all the information requested in this section for those sites as attachments. -7 

Mark (X) this box if the data continues on the next page. 0 

b. Type-­ Manufacturing 0 Mark (X) Processing [XJ Use 0 0 c. Amount and Duration -­ Complete 1 or 2 as appropriate Confidential 

Maximum kg/batch 

(100% new chemical Hours/batch Batches/year 

1. Batch substance) 0

Maximum kg/day Hours/day Days/year 

2. Continuous (100% new chemical substance) [XJ XXX XXX XXX 

Mark (X) to indicate your willingness to 

d. Process description have your process description binding. 0 -7 

(1) Diagram the major unit operation steps and chemical conversions. Include interim storage and transport containers (specify- e.g. 5 gallon 

pails, 55 gallon drum, rail car, tank truck, etc.). 

(2) Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting 

materials and feedstocks (including reactants, solvents, catalysts, etc.), and of all products, recycle streams, and wastes. Include cleaning 

chemicals (note frequency if not used daily or per batch.). 

(3) Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If 

releasing to two media at the same step, assign a second release number for the second medium. 

0 

See Processing Diagram Attachment 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 18 

SANITIZED SUBMISSION 1111111111111111111111111111111111111111111111111111111111111111111111 

PMN2010P8AX1 PMN 


of the major unit operation steps. 


Attachment filename for Part II, 


EPA FORM 7710-25 (Rev 6-09) Replaces previous editions of EPA Form 7710-25 


Page 19 

SANITIZED SUBMISSION II1II111I1111II11111111111111111I11111111111 

PMN2010P9X1 .. PMN Page 9 ... ...~--~---~----c 

6 

,------ Part II·· HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE •• Continued 


Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUBMIITER _. Con~t==-in=u=ed=-===--__
 .­

The information on pages 9 and 9a refer to consolidated chemical number(s): 01 02 03

2. Occupational Exposure -- You must make separate confidentiality claims for the description of worker activity. physical form of the new chemical 

substance. number of workers exposed, and duration of activity, Mark (X) the "Confidential" box next to any item you claim as confidential. 

(1) 	 Describe the activities (I.e, bag dumping, tote filling. unloading drums, sampling, cleaning, etc,) in which workers may be exposed to the 

substance, 

(2) 	 Mark (X) this column if entry in column (1) is confidential business information (CBI), 

(3) --	 Describe any protective equipment and engineering controls used to protect workers, 

(4) and (6) -- Indicate your willingness to have the information provided in column (3) or (5) binding, 

(5) 	 -- Indicate the physical formes) of the new chemical substance (e,g" solid: crystal, granule, powder, or dust) and % new chemical substance (if 

part of a mixture) at the time of exposure, 

(7) -- Mark (X) this column if entries in columns (3) and (5) are confidential business information (CBI), 

(8) 	 Estimate the maximum number of workers involved in each activity for all sites combined, 

(9) 	 Mark (X) this column if entry in column (8) is confidential business information (CBI), 

(10) and (11) -- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

(12) -- Mark (X) this column if entries in columns (10) and (11) are confidential business information (CBI), I 

! 

Worker activity Protective Equipment/ Binding Physical Binding # of Maximum Duration 

(i.e" bag dumping, filling CBI Option form(s) Option CBI Workers CBI eBI 

drums) Engineering Controls Mark (X) &%new Mark (X) Exposed 

(1) (2) (3) (4) sUb~ltance (6) (7) (8) (9) Hrs/Day DayslYr (12) 

5) (10) (11) iW.;gh;09 PMNoh.m;,,' See MSDS Dry solid 99­ 6 0.7 60 

and discarding waste i 100% 

from empty container 

I 

, Loading the mix vessel See MSDS Dry solid 99­ 6 0,4 60 

100% 

Overseeing solution See MSDS Solution 6 0,7 I 60 

preparation and sampling -0,12% 

I I 

Discarding waste from See MSDS Solution 9 I 2 

I 

60 

i filtering equipment 

I 

-0.12% 

I I 

I 

I I 

I 

I 

I I 

I I 

I 

: I 

I 

I 

I 

I 

I 

I 

I 

I 

I 

I 

Mark (X) this box if the data continues on the next page I I 

AnCl(;runem , Section I the bottom of page 9a [ ] 

! 

I 

EPA FORM 7710-25 (Rev, 6-09) Replaces previous editions of EPA Form 7710-25 

Page 20 
SANITIZED SUBMISSION 11111111111111111111111111111111 11111111111111111111111111 

PMN2010P9AX1 PMN P 9a 

Navigable waterway- I 

age 

3. Environmental Release and Disposal You must make separate confidentiality claims for the release number and the amount of the new chemical

substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(1) -- Enter the number of each release point identified in the process description. part II, section A, subsection 1d(3). 

(2) -- Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) -- Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). 

(4) Identify the media (stack air, fugitive air (optional-see Instruction Manual), surface water. on-site or off-site land or incineration, POTW, or 

other (specify)) to which the new substance will be released from that release point 

(5) a. Describe control technology, if any, and control efficiency that will be used to limit the release of the new substance to the environment. 

For releases disposed of on land. characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste, 

On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to 

secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day), 

(6) -- Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) -- Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct 

discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES # is confidential 

business information (CBI), 

Release Amount of New Medium of release Control technology and efficiency (you may wish to 

Number Substance Released CBI e.g. Stack air optionally attach efficiency data) CBI 

(1 ) (2a) (2b) (3) (4) 

Binding 

(Sa) Mark (5b) (6) 

{X} 

I 

i 

1 <0.1 kg i Other: Landfill 

i 2 < 0.1 kg i Recovery with disposal of non- < 0.1 kg 

I 

recoverables in compliance with 

i regulations 

3 < 0.1 kg 

I I 

i Incineration 0 

I 

i ,..--... 

I 

i 

I 

I ! 

I I ! 

I 

I 

! 

i i 

Mark (X) this box if the data continues on the next page. ! D 

(7) Mark (X) the destination(s of releases to water. NPDES# CBI 

POTW--provide 

name(s) 

I D . . 

D Other--Specify D 

Enter Attachment filename for Part II, Section A. D 

EPA FORM 7710-25 (Rev. 6-09) Replaces previOUS editions of EPA Form 7710-25 

Page 21 
i 

I Section A -- INDUSTRIAL SITES CONTROLLED BY THE SUBMITT=E=;R_.........__ --.!~z....:=~~~~F-"-'-'~~---i 

11111111111111111111111111111111111111111111111111111111111111111 SAN ITIZE D SUB MISS ION 

PMN2010P8X2 PMN pa~-,,--==-c=-:c-::--=-::::-:-:=----=--::-=::--- ____---, 

1--- Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE 

I The information on pages 8 and 8a refer to consolidated chemica':;;.I.::.:n.=u:;;.m:;:b..:;.9;:.,!r(.:,;S)C'-:-::--=0=:"'-..:;1--:-:--"0""""'_2.----"=:='.....::..,,.--:1==;'-_:----'=="'-:--:----:'7=:'--::----\ 

Complete section A for each type of manufacture, processing, or use operation involving the new chemical substance at industrial sites 

, you control. Importers do not have to complete this section for operations outside the U.S.; however, you may still have reporting 

I requirements if there are further industrial processing or use operations after import, You must describe these operations. See 

lJDstructions manual--:-_____________________________________--,_,.---::-----i 

, 1, 	 Operation description Confia. Identlt Enter the identity of the site at which the operation will occur, .___ .____________+ __d..:..en_t_ia_1-1 

Name Graphics Communications Group of Eastman Kodak Company :-------------+-~---

Site address (number a 


~------------~ 

street) 
 One Polychrome Park, Corporate -~--~--------------------i 

Ridge Industrial Park o

C;ly 	 Em,", C,"oly IM,,,ogoo

State ... GA.. ZIP code l31907 


If the same operation will occur at more than one site, enter the number of sites, Identify the additional 


sites on a continuation sheet, and if any of the sites have significantly different production rates or 1 o erations, include all the information re uested in this section for those sites as attachments, -7 

Mark (Xl this box if the data continues on the next page. 

b. Type-­

Mark (X) Manufacturing 0 Processing Use 

o 

o 

o 

o 

c, Amount and Duration -- Complete 1 or 2 as appropriate 

Maximum kg/batch 

(100% new chemical Hours/batch Batches/year 

1, Batch substanc~) 0 

Maximum kg/day Hours/day Days/year (100% new chemical substance) 2. Continuous [R] XXX XXX XXX I 

Mark (X) to indicate your willingness to 


d, Process description 
 have your process description binding, 0 -7 	 I i 

,.---' (1 ) Diagram the major unit operation steps and chemical conversions, Include interim storage and transport containers (specify- e,g. 5 gallon 

pails, 55 gallon drum, rail car, tank truck, etc.), 

(2) 	 Provide the identity, the approximate weight (by kg/day or kg/batch on a 100% new chemical substance basis), and entry point of all starting 

materials and feedstocks (including reactants, solvents, catalysts, etc,), and of all products, recycle streams, and wastes, Include cleaning 

chemicals (note frequency if not used daily or per batCh,), 

(3) 	 Identify by number the points of release, including small or intermittent releases, to the environment of the new chemical substance. If 

m releasing to two media at the same stEl~s:sign a second release number for the second medium, 

0 

See Coating Operation Diagram Attachment 

EPA FORM 7710-25 (Rev, 6-09) Replaces previous editions of EPA Form 7710-25 

Page 22 
I 

~ 11111111111111111111111111111111 11111111111111111111111 SANITIZED SUBMISSION 

PMN2010PBAX2 PMN Page 8a 

Confidential 

~. I Diagram of the major unit operation steps. D 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 23 

11111111111111111111111111111111111111111111 SANITIZED SUBMISSION 

PMN2010P9X2 PMN Page 9 

I Part 11·- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE - Continued I 

Section A _. INDUSTRIAL SITES CONTROLLED BY THE SUBMITIER _. Continued 

The infonnation on pages 9 and 9a refer to consolidated chemical number(s): 01 [ 2 03 04 05 06 

2. Occupational Exposure -- You must make separate confidentiality claims for the description of worker activity, physical form of the new chemical 

substance, number of workers exposed, and duration of activity. Mark (X) the "Confidential" box next to any item you claim as confidential. 

(1 ) Describe the activities (Le. bag dumping, tote filling, unloading drums, sampling, cleaning, etc.) in which workers may be exposed to the 

substance. 

(2) -­ Mark (X) this column if entry in column (1) is confidential business information (CBI). 

(3) -­ Describe any protective equipment and engineering controls used to protect workers. 

(4) and (6) -­ Indicate your willingness 10 have the information provided in column (3) or (5) binding. 

(5) Indicate Ihe physical formes) of the new chemical substance (e.g., solid: crystal, granule, powder, or dust) and % new chemical substance (if 

part of a mixture) at the time of exposure. 

(7) -­ Mark (X) this column if entries in columns (3) and (5) are confidential business information (CBI). 

I (8) Estimate the maximum number of workers involved in each activity for all sites combined. 

(9) Mark (X) this column if entry in column (8) is confidential business information (CBI). 

(10) and (11) -- Estimate the maximum duration of the activity for any worker in hours per day and days per year. 

(12) -­ Mark (X) this column if entries in columns (10) and (11} are confidential business information (CBI). 

Worker activity Protective Equipment! Binding Physical Binding #of Maximum Duration (i.e., bag dumping, filling CBI Option form(s) Option CBI Workers CBI CBI 

I 

drums) Engineering Controls Mark (X) &%new Mark (X) Exposed 

(1) (2) (3) (4) substance (6) (7) (8) (9) Hrs/Day DaysNr (12) . 

(5) (10) (11) 

i Discarding waste from . See MSDS Solution i 10 2 35 ! 

filtering equipment ! : -0.12% 

I 

i 

! 

I 

I I 

I I 

I 

i 

i Overseeing coating ! See MSDS Solution - ! 10 4 35 

• operation and i 0.12% i 

I maintenance of the 

equipment 

I 

I ! 

: 1

i 

I I J i J i i I 

I 

i 

I I I 

I 

I i I I 

I 

I I i I 

I i 

I 

I 

I 

I 

I 

i I i 

I i I 

I i 

I i

I i I i 

i 

I i i I i 

I 

I I 

: 

I i j i 

! 

I 

. 

I I i

i 

i I i I 

Mark (X) this box if the data continues on the next page. I I 

Enter Attachment filename for Part II. Section A on the bottom of page 9a. [ ] 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 24 
11111111111111111111111111111111111/11111111/11111111111111111111/1111 SANITIZED SUBMISSION 

PMN2010P9AX2 PMN Page 9a 

I 

I 

I 

3. Environmental Release and Disposal -­ You must make separate confidentiality claims for the release number and the amount of the new chemical 

substance released and other release and disposal information. Mark (X) the "Confidential" box next to each item you claim as confidential. 

(1) -­ Enter the number of each release point identified in the process description, part II. section A. subsection 1d(3). 

(2) -­ Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology (in kg/day or kg/batch). 

(3) -­ Mark (X) this column if entries in columns (1) and (2) are confidential business information (CBI). 

(4) Identify the media (stack air, fugitive air (optional-see Instruction Manual). surface water, on-site or off-site land or incineration, POTW, or 

other (specify)) to which the new substance will be released from that release point. 

(5) -­ a. Describe control technology, if any. and control efficiency that will be used to limit the release of the new substance to the environment. 

For releases disposed of on land, characterize the disposal method and state whether it is approved for disposal of RCRA hazardous waste. 

On a continuation sheet, for each site describe any additional disposal methods that will be used and whether the waste is subject to 

secondary or tertiary on-site treatment. b. Estimate the amount released to the environment after control technology (in kg/day). 

(6) -­ Mark (X) this column if entries in columns (4) and (5) are confidential business information (CBI). 

(7) Identify the destination(s) of releases to water. Please supply NPDES (National Pollutant Discharge Elimination System) numbers for direct 

discharges or NPDES numbers of the POTW (Publicly Owned Treatment Works). Mark (X) if the POTW name or NPDES # is confidential 

business information (CBI). 

Release Amount of New 

Substance Released Medium of release Control technology and efficiency (you may wish to 

optionally attach efficiency data) Number CBI e.g. Stack air 

(1 ) (2a) (2b) (3) (4) 

1 < 0.01 kg Other: Landfill 

2 < 0.01 kg 

I 

3 

(Sa) 

I 

Recovery with disposal of non­ , 

recoverables in compliance with I' 

: regulations 

Incineration 

Binding 

Mark 

(X) 

CBI 

(5b) (6) 

I 

" 

I < 0.01 kg I 

I <001 kg o

I i 

I I 

I 

! 

I I 

i I 

I I 

i 

I 

I 

I 

Mark (Xl this box if the data continues on the next page. 

I (7) Mark (X) the destination(s) of releases to water. 

POTW--provide 

name(s) 

Navigable waterway­

- provide name(s) 

C Other--Specify 

I 

i 

I ! 

I I I 

I 

I , 

I 

I 

o 

NPDES# I eBI 

!o 

0 1 

Enter Attachment filename for Part II, Section A, 0' 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 25 

1111111111111111111111111111111111111111111111111111111111II 	 SANITIZED SUBMISSION 

PMN2010P10 	 PMN Pa e 10 

Part 11-- HUMAN EXPOSURE AND ENVIRONMENTAL RELEASE - Continued 

-" Section B -- INDUSTRIAL SITES CONTROLLED BY OTHERS

The information on pages 10 and 10a refer to consolidated chemical number(s): D 1 [12 D 3 D 4 D 5 D 6 I Complete section B for typical processing or use operations involving the new chemical substance at sites you do not control. Importers do not have to 

i complete this section for operations outside the U.S; however, you must report any processing or use activities after import. See the Instructions Manual. 

Complete a separate section B for each type of processing, or use operation involving the new chemical substance. If the same operation is performed at I more than one site describe the typical operation common to these sites. Identify additional sites on a continuation sheet. 


1(a). Operation Description -- To claim information in this section as confidential, bracket (e.g. mthe specific information that you claim as 


confidential. 


(1) 	-- Diagram the major unit operation steps and chemical conversions, including interim storage and transport containers (specify - e.g. 5 gallon 

pails, 55 gallon drums, rail cars, tank trucks, etc). On the diagram, identify by leiter and briefly describe each worker activity. 

(2) 	-- Either in the diagram or in the text field 1(b) below, provide the identity, the approximate weight (by kg/day or kg/batch. on an 100% new 

chemical substance basis), and entry point of all feedstocks (including reactants, solvents and catalysts, etc) and all products, recycle 

streams, and wastes. Include cleaning chemicals (note frequency if not used daily or per batch). 

(3) --	 Either in the diagram or in the text field 1 (b) below, identify by number the points of release, including small or intermittent releases, to the I environment of the new chemical substance. 

i (4) -- Please enter the # of sites (remember to identify the locations of these sites on a continuation sheet): 

I 	 I Number of Sites I 0 I Confidential D 

1(b). (Optional) This space is for a text description to clarify the diagram above. 	 Confidential D 

Enter Attachment filename for Part II, Section B on the bottom of page 10a. I D 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 26 

I 
11111111111111111111111111111111 11111111111111111111111 SANITIZED SUBMISSION 

PMN2010P10·1 

Continuation Sheet 

.' ID IP10SB1(a)(4)1 I Field I Part II, Section B, 1(a)(4). Operation Site Locations 

Not applicable 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 


Page 27 

1111111111111111111111111111111111111111111111111111 1111111111111 	 SANITIZED SUBMISSION 

PMN2010P10A 	 PMN Pa e 10a 

2. Worker Exposure/Environmental Release 

(1) 	 From the diagram above, provide the lelter for each worker activity. Complete 2-8 for each worker activity described. 

(2) 	 Estimate the number of workers exposed for all sites combined. 

(4) --	 Estimate the typical duration of exposure per worker in (a) hours per day and (b) days per year. 

(6) 	-- Describe physical form of exposure and % new chemical substance (if in mixture), and any protective equipment and engineering controls, if 

any, used to protect workers. 

(7) 	 Estimate the percent of the new substance as formulated when packaged or used as a final product. 

(9) -­	 From the process diagram above, enter the number of each release pOint. Complete 9-13 for each release point identified. 

(10) -- Estimate the amount of the new substance released (a) directly to the environment or (b) into control technology to the environment (in 

kg/day or kg/batch). 

(12) -- Describe media of release Le. stack air, fugitive air (optional-see Instructions Manual). surface water, on-site or off-site land or incineration, 

POTW, or other (specify) and control technology, if any, that will be used to limit the release of the new substance to the environment. 

I 


(14) -- Identify byproducts which may result from the operation. 


(3), (5), (8), (11), (13) and (15) -- Mark (X) this column if any of the proceeding entries are confidential business information (CBI). 


(14) Byproducts: o 

! 

I 

I 

I 

I 

I 

: 

I 

I 

Letter # of 

of Workers CBI Duration of CBI Protective Equlp.fEngineering Controls/Physical % new %in CBI 

I ! 

Activity Exposed Exposure Form substance Formulation 

(1) (2) (3) (4a) (4b) (5) (6) (6) (7) (8) 

I I ! 

i i I 

I 

I 

J i 

! 

I ! I 

I i 

I I I i 

I 

I ! 

I i I I 

! ! 

I 

I i 

! I 

I I I i I 

Release Amount of New Substance Released CBI Media of Release & Control Technology CBI Number 

(9) (10a) I (10b) (11) (12) (13) 

i i 

Mark (X) this box if the data continues on the next page. 

I 

I 

Enter Attachment filename for Part II, Section B. o 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 28 
111111111111111111111111111111111111111111111111111111111111 SANITIZED SUBMISSION 

PMN2010P11 PMN Pa e 11 

OPTIONAL POLLUTION PREVENTION INFORMATION 


To claim information in the follOwing section as confidential, bracket (e,g, {}) the specific information that you claim as confidential, 

In this section you may provide information not reported elsewhere in this form regarding your efforts to reduce or minimize potential risks 

associated with activities surrounding manufacturing, processing, use and disposal of the PMN substance, Please include new information 

pertinent to pollution prevention, including source reduction, recycling activities and safer processes or products available due to the new chemical 

substance, Source reduction includes the reduction in the amount or toxicity of chemical wastes by technological modification, process and 

procedure modification, product reformulation, and/or raw materials substitution, Recycling refers to the reclamation of useful chemical components 

from wastes that would otherwise be treated or released as air emissions or water discharges, or land disposal. Quantitative or qualitative 

descriptions of pollution prevention, source reduction and recycling should emphasize potential risk reduction in addition to compliance with existing 

regulatory requirements, The EPA is interested in the information to assess overall net reductions in toxiCity or environmental releases and 

exposures, not the shifting of risks to other media (e,g" air to water) or nonenvironmental areas (e,g" occupational or consumer exposure), To the 

extent known, information about the technology being replaced will assist EPA in its relative risk determination, In addition, information on the 

relative cost or performance characteristics of the PMN substance to potential alternatives may be provided, 

Describe the expected net benefits, such as 

(1) an overall reduction in risk to human health or the environment; 

(2) a reduction in the generation of waste materials through recycling, source reduction or other means; 

(3) a reduction in the use of hazardous starting materials, reagents, or feedstocks; 

(4) a reduction in potential toxicity, human exposure and/or environmental release; or 

(5) the extent to which the new chemical substance may be a substitute for an existing substance that poses a greater overall risk to human 

health or the environment. 

Information provided in this section will be taken into consideration during the review of this substance. See PMN Instructions Manual 

and Pollution Prevention Guidance manual for uidance and exam les. 

EPA FORM 7710-25 (Rev, 6-09) Replaces previous editions of EPA Form 7710-25 

Page 29 
1111111111111111111111111111111111111111111111 SANITIZED SUBMISSION 

PMN2010P12 PMN Page 12 

Part III -- LIST OF ATTACHMENTS 

Attach continuation sheets for sections of the form, test data and other data (including physical/chemical properties and 

structure/activity information), and optional information after this page. Clearly identify the attachment and the section of the form 

to which it relates, if appropriate. Number consecutively the pages of any paper attachments. In the Number of Pages column 

below, enter the inclusive page numbers of each attachment for paper submissions or enter the total number of pages for each 

attachment for electronic submissions. Electronic attachments can be identified by filename. 

Mark (X) the "Confidential" box next to any attachment name or filename you claim as confidential. Read the Instructions Manual 

for guidance on how to claim any information in an attachment as confidential. You must include with the sanitized copy of the 

notice form a sanitized version of any attachment in which you claim in f ormation as con fi' Identla I. 1-1.. INumber 

Associated 

Attachment Name Attachment Filename ICBI i of Pages PMN Section 

Number 

001 IES Report i 1 Pt.!. Sec.B, k I 

i 

1

002 IKodak PMN Substance Structure 11 PLI, Sec.B, 1e. 1 

1003 Process Diagram 3 Pt.2, Sec.A. 1d. 

1 I i 

I i Processing Diagram I 

1 Pt.2, SecA 1d. (#2) I 

I 

1 

005 ICoating Operation Diagram 1 Pt.2, Sec.A, 1d. (#3) I i 

006 1Material Safety Data Sheet 7 

I I 

007 IStudy 16 

I i I 

008 Analysis 3 

1 

I 

009 FT-IR Analysis 1 I 

I 

1 

010 Proton NMR Analysis 2 i 

1011 IParticle Size Analysis 6 

I I 

1012 IDSC Analysis 11 i 

,013 1Chemical Safety Data Sheet 12 I 

1014 Acute Toxicity to Zebra Fish S0507 ! 28 

I I 

1015 ,Ready Biodegradation Test S0507 i 116 i 

I ! 

016 IPMN Fee Payment Acknowledgement i 1 i

I • 1 

1 i 1 

I i 1 

I 

1 

I I 

Mark (X) this box if the data continues on the next page. l J 

EPA FORM 7710-25 (Rev. 6-09) Replaces previous editions of EPA Form 7710-25 

Page 30 
111111111111111111111111111111111111111111111111111111111111 SANITIZED SUBMISSION 

PMN2010P13 

PMN P age 13 


PHYSICAL AND CHEMICAL PROPERTIES WORKSHEET 

The information on this page refers to chern I Is r 16 

To assist EPA's review of physical and chemical properties data, please complete the following worksheet for data you provide and include it in the 

notice. Identify the property measured. the value of the property, the units in which the property is measured (as necessary). and whether or not the 

property is claimed as confidential. Give the attachment number (found on page 12) in column (b). The physical state of the neat substance should be 

provided. These measured properties should be for the neat (100% pure) chemical substance. Properties that are measured for mixtures or 

formulations should be so noted (% PMN substance In _I. You are not required to submit this worksheet; however, EPA strongly recommends that 

you do so. as it will simplify the review and ensure that confidential information is properly protected. You should submit this worksheet as a 

supplement to your submission of test data. This worksheet is not a substitute for submission of test data. 

Property Unit I Mark X if Altac Value Measured CBI 

Nu or Estimate Mark (X) (a) Provided (e) (M or E) (d) 

D (solid) I (liquid) (gas) 

Physical state of neat substance [X] D D Measured 

Vapor Pressure @ 25 D 5,01 E-021 Torr Estimate 

Temperature 

·c 

Density/relative density D g1em3 

Solubility 

@ Temperature ·c D gIL 

Solvent 

Solubility 0.00009 in Water @ D gIL Temperature 

·c 

Melting Temperature D j4l:l 

·c 

I 

Boiling 1Sublimation 898 : Estimate D I 

temperature @ Torr ! ·C 

D H1 NMR 

Spectra 

Dissociation constant D 

Octanoll water partition coefficient D I 6,02 

Estimate 

Henry's Law constant D 

Volatilization from water D 

Volatilization from soil D 

pH@ concentration D 

Flammability D 

Explodability D 

Adsorption I Coefficient D 

Particle Size Distribution D Measured 

I Other Specify HPLC purity D 99 area % 

I Measured 

Page 31 

ATTACHMENT HEADER SHEET 


Attachment Number 001 

Attachment Name 


IES Report 


Associated PMN Section Number 


Pt.l, Sec.B, 1c. 


Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 32 
Sanitized Version 

Inventory Expert Service 


A division of the American Chemical Society Phone:~631-1884,61~7-3870 

Fax: 614-447-3747 

E-mail: answers@cas.org 

Web: www.cas.orglproducts/dientl 

INVENTORY EXPERT SERVICE REPORT 


:ES-ORDER NUMBER: 

REGISTRY NUMBER: 401 0 2=-7T'::~f-l 

""""".. -"'~,~'''~ 

CA INDEX NAME 

2-[2-[3-[2-(1,3-dihydro-l,3,3-trimethyl-2H-indol-2-ylidene)ethylidene]­

2-[ (1-phenyl-1H-tetrazol-5-yl)thio]-1-cyclohexen-1-yl]ethenyl]-1,3,3­

trimethyl-,.. chloride 1:1 -- ,.,.-,.....,."""""'''~ 

Please print the above CA Index Name on the appropriate page of your PMN. 

o If this box is checked, CAS has made correction(s) marked in red to 

your IES order. Please make the same correction(s) to your PMN before 

submitting it to the EPA. 

CAS 'lS~ O\entlJngy I\i'ver I\oad P.O. Sox :n":l Columbus. Ohio '\:l'l11)..l):l:l4 

Attachment 1, Page 33 
ATTACHMENT HEADER SHEET 


Attachment Number 002 

Attachment Name 


Kodak PMN Substance Structure 


Associated PMN Section Number 


Pt.!, Sec.B, 1e. 


Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 34 
Sanitized Version 

PMN Substance Structure 


CASRN 440102-72-7 

3H-Indolium, 2-[2-[3-[2-( I ,3-dihydro-1 ,3,3-trimethyl-2H-indol-2-ylidene)ethylidene ]-2­

[I-(phenyl-I H-tetrazol-5-yl)thio ]-l-cyclohexen-l-yl]ethenyl]-l ,3,3-trimethyl-. chloride 

Attachment 2, Page 35 
ATTACHMENT HEADER SHEET 


Attachment Number 003 

Attachment Name 


Process Diagram 


Associated PMN Section Number 


Pt.2, Sec.A, 1d. 


Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 36 
Manufacturing Process Diagram Sanitized Version 

Attachment 3, Page 37 

Sanitized Version 

REACTION CHEMICALS AMOUNT MOLES 


Attachment 3, Page 38 

Chemistry Flow Diagram Sanitized Version 

CASRN 440102-72-7 

3H-1 ndolium, 2-[2-[3-[2-( 1.3-dihydro-1 ,3, 3-trimethyl-2H-indol-2-ylidene )ethylidene ]-2-[(phenyl-1 Htetrazol-5-yl)thio]-1-cyclohexen-1-yl]-1.3,3-trimethyl-. chloride 

Attachment 3, Page 39 
ATTACHMENT HEADER SHEET 


Attachment Number 004 

Attachment Name 


Processing Diagram 


Associated PMN Section Number 


Pt.2, Sec.A, 1 d. (#2) 


Does not contain CBI 

Report Number 


KODA101220491061974 


Page 40 
Sanitized Version 

Processing Diagram 

PMN chemical 440102-72-7 Weighing

0.8 kg/batch 1 to 2 batches per day equipment 

Resid al PMN chemical from container 

liner 

Water [7732-18-5J 42.~,-,k=-Q_----;1101 

Other addenda 

Release 

Mixed solvent cleaninO"'--___.....-...I Mix vessel I Mixed solvent cleaning liquors 2 

or 

3 

Release 3 

! I Release Cleaning (Le. residue) Filtration 1 

equipment or 

3 

Coating operation 

0.12% Solution 

Key 

Release 1 = Landfill 

Release 2 = Recovery 

Release 3 = Incineration 

Mixed solvent: 


2-Butanone (78-93-3) 45% 


Water (7732-18-5) 10% 


1-Methoxy-2-Propanol (CAS 107-98-2) 35% 


4-Butrolactone (CAS 96-48-0) 10% 


Attachment 4, Page 41 
ATTACHMENT HEADER SHEET 


Attachment Number 005 

Attachment Name 


Coating Operation Diagram 


Associated PMN Section Number 


Pt.2. Sec.A. 1d. (#3) 


Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 42 
coating 

ontinous 24 hour operation. There 

re 

rovide 

. oating machine. It takes 12 hours 

. 0 

ollowing 

aterial 

ill be captured 

xhaust and incinerated or will be 

we t u 

Sanitized Version 

Coating Operation 

PMN chemical [440102-72-7] 

0.8 k /batch as a 0.12% solution of 

PMN chemical 

1 to 2 batches per day Holding 

vessel 

Mixed solvent clean",in~__~.., r-M=ix=e:..:::dc...:s::.;:o:.:.;lv:..,:e:..:.,n:.:..t-=.c,-",Ie=a"-,n~in""'g--'-l=i9c=u-"-o'-=rs'--_.... Release 3 

Filtration 

equipment 

Release 

Cleaning (i.e. residue) 1 

or 

3 

Solution from coatin 0 eration Release 3 Coating 

Mixed solvent cleaning equipment Release 1_--,-,-M=ix=e""d:...;s::.:o:..:.lv.:...:e:..:.n:.::.t-=c:.:.=le:.:a:.:..;n""in"",gL.;l=iqL::u:.=o.:..:rs"----)100-" 2 

or 3 

Article 

operation is a 

multiple holding tanks used to 

Key coating solution to one 

Release 1 = Landfill 

Release 2 =Recovery coat one batch. Dust emissions 

Release 3 = Incineration unloading of solid 

from transport container 

in filters of local 

Mixed solvent: 

2-Butanone (78-93-3) 45% and incinerated. 

Water (7732-18-5) 10% 

1-Methoxy-2-Propanol (CAS 107-98-2) 35%, 

4-Butrolactone (CAS 96-48-0) 10% 

Attachment 5, Page 43 
ATTACHMENT HEADER SHEET 


Attachment Number 006 

Attachment Name 


Material Safety Data Sheet 


Associated PMN Section Number 

N/A 

Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 44 
Sanitized Version 

Material Safety Data Sheet 

Revision Date: 11/15/2010 

000000033729Nersion: 1.1 Kodak 

Print Date: 12/01/2010 

• 1. Identification of the substance/mixture and of the company/undertaking 

Product name: ORM1835 


Product code: 10091357 


Supplier: EASTMAN KODAK COMPANY, 343 State Street, Rochester, New York 14650 


For Emergency Health, Safety & Environmental Information, call (585) 722-5151 (USA) 


For further information about this product, call (800) 242-2424. 


Synonyms: None. 


Product Use: Plate manufacturing chemical, For industrial use only. 


CONTAINS: S 0507 IR Dye (proprietary) 

WARNING! 

POWDERED MATERIAL MAY FORM EXPLOSIVE DUST-AIR MIXTURES 

HEAT SENSITIVE - CAN DECOMPOSE IF HEATED 

MAY BE HARMFUL IF SWALLOWED 

MAY CAUSE SKIN AND EYE IRRITATION 

DUST MAY BE IRRITATING TO THE EYES AND RESPIRATORY TRACT 

THE PHYSICAL-CHEMICAL AND TOXICOLOGICAL PROPERTIES OF THIS MATERIAL HAVE NOT 

BEEN FULLY INVESTIGATED 

HMIS III Hazard Ratings: Health - 1, Flammability - 1, Reactivity (Stability) - 2 

NFPA Hazard Ratings: Health - 3, Flammability - 1, Instability - 2 

NOTE: HMIS III and NFPA 704 (2007) hazard indexes involve data review and interpretation that may 

vary among companies. They are intended only for rapid, general identification of the magnitude of the 

potential hazards. To adequately address safe handling, ALL information in this MSDS must be 

considered. 

I 3. Composition/information on ingredients 

Weight Components - (CAS-No.) 

percent 

100 S 0507 IR Dye (proprietary) 

Inhalation: If inhaled, remove to fresh air. Get medical attention if symptoms occur. 

Attachment 6, Page 45 
Sanitized Version 

Material Safety Data Sheet 

Revision Date: 11/15/2010 

000000033729Nersion: 1.1 Kodak 

Print Date: 12/01/2010 

Eyes: Any material that contacts the eye should be washed out immediately with water. Get medical 

attention if symptoms occur. 

Skin: Wash off with soap and water. Get medical attention if symptoms occur. 

Ingestion: If swallowed, only induce vomiting as directed by medical personnel. Never give anything by 

mouth to an unconscious person. Never give anything by mouth to an unconscious person. Call a 

physician or poison control centre immediately. 

Extinguishing Media: Water spray, Carbon dioxide (C02), Dry chemical. Use water spray to cool 

unopened containers. 

Special Fire-Fighting Procedures: Wear self-contained breathing apparatus and protective clothing. 

Fire or excessive heat may produce hazardous decomposition products. 

Hazardous Combustion Products: Carbon oxides, Sulphur oxides, nitrogen oxides (NOx), (see also 

Hazardous Decomposition Products sections.) 

Unusual Fire and Explosion Hazards: Elevated temperature can cause decomposition. Dust may form 

explosive mixture in air. 

Shovel into suitable container for disposal. Avoid dust formation. Clean surface thoroughly to remove 

residual contamination. 

7. 

Personal precautions: Avoid breathing dust. Avoid contact with eyes, skin, and clothing. Use only with 

adequate ventilation. Wash thoroughly after handling. 

Prevention of Fire and Explosion: Keep away from heat and sources of ignition. Exercise caution if 

heating, especially in a closed container. Dust may form explosive mixture in air. Minimize dust 

generation and accumulation. Use only with adequate ventilation. Refer to NFPA 654, "Standard for the 

Prevention of Fire and Dust Explosions from the Manufacturing, Processing, and Handling of Combustible 

Particulate Solids." Keep from contact with oxidizing materials. 

Storage: Keep container tightly closed. Keep away from incompatible substances (see Incompatibility 

section.) 

• 8. ExpC?sure controls/personal protection 

Occupational exposure controls: Not established 

Ventilation: Good general ventilation should be used. Ventilation should be sufficient so that applicable 

occupational exposure limits are not exceeded. Ventilation rates should be matched to conditions. 

Supplementary local exhaust ventilation, closed systems, or respiratory protection may be needed in 

special circumstances. 

Attachment 6, Page 46 
Sanitized Version 

Material Safety Data Sheet 

Revision Date: 11/15/2010 

000000033729Nersion: 1.1 Kodak 

Print Date: 12/01/2010 

Respiratory protection: None should be needed. 


Eye protection: Wear safety glasses with side shields (or goggles). 


Hand protection: For operations where prolonged or repeated skin contact may occur, impervious 


gloves should be worn. 

I 9. Physical and chemical properties 

Physical form: solid (crystalline) 

Colour: green 

Odou r: odourless 

Specific gravity: 0.15 - 0.27 

Vapour pressure: no data available 

Vapour density: no data available 

Volatile fraction by weight: not applicable 

Melting point/range: no data available 

Water solubility: insoluble 

pH: not applicable 

Flash point: not applicable 

Stability: Not fully evaluated. Materials containing similar structural groups can decompose if heated. 


Incompatibility: Strong acids, Bases, light. 


Hazardous decomposition products: nitrogen oxides (NOx), Sulphur oxides, hydrogen chloride 


Hazardous Polymerization: Hazardous polymerisation does not occur. 


information 

Effects of Exposure 


General advice: The toxicological properties of this chemical have not been fully investigated. 


Inhalation: Airborne dust/mist/vapor may be irritating. 


Eyes: May cause eye irritation. Airborne dust/mist/vapor may be irritating. 


Attachment 6, Page 47 
Sanitized Version 

Material Safety Data Sheet 

Revision Date: 11/15/2010 

000000033729Nersion: 1.1 Kodak 

Print Date: 12/01/2010 

Skin: May cause skin irritation. 

Ingestion: May be harmful if swallowed. 

The following properties are ESTIMATED from the components of the preparations. 

Potential Toxicity: 

Toxicity to fish (LC50): > 100 mgtl 

Toxicity to daphnia (EC50): > 100 mgtl 

Persistence and degradability: Not readily biodegradable. 

13. considerations 

Discharge, treatment, or disposal may be subject to federal, state, commonwealth, provincial, or local 

laws. Since emptied containers retain product residue, follow label warnings even after container is 

emptied. 

Not regulated for all modes of transportation. 


For more trans ortation information, go to: www.kodak.com{9.___
 ots--.h--.-ip_.____________--. 

15. Regulatory information ------------------------------------~ 

Notification status 

Regulatory List Notification status 

TSCA Not all listed 

DSL Not all listed 

NDSL None listed 

EINECS Not all listed 

ELiNCS None listed 

NLP None listed 

AICS Not all listed 

IECS Not all listed 

ENCS Not all listed 

ECI Not all listed 

NZloC Not all listed 

Attachment 6, Page 48 
Sanitized Version 

Material Safety Data Sheet 

Revision Date: 11/15/2010 

000000033729Nersion: 1.1 Koda 

Print Date: 12/01/2010 

PICCS Not all listed 

"Not all listed" indicates one or more component is either not on the public Inventory or is subject to 

exemption requirements. If additional information is needed contact Kodak. 

Other regulations 

American Conference of Governmental Industrial Hygienists 

(ACGIH): 

International Agency for Research on Cancer (IARC): 

U.S. National Toxicology Program (NTP): 

U.S. Occupational Safety and Health Administration 

(OSHA): 

California Prop. 65 

U.S. - CERCLAISARA (40 CFR § 302.4 Designation of 

hazardous substances): 

U.S. - CERCLA/SARA - Section 302 (40 CFR § 355 

Appendices A and B - The List of Extremely Hazardous 

Substances and Their Threshold Planning Quantities): 

U.S. - CERCLAISARA - Section 313 (40 CFR § 372.65 

Toxic Chemical Release Reporting): 

U.S. - California - 8 CCR Section 339 - Director's List of 

Hazardous Substances: 

U.S. - California - 8 CCR Section 5200-5220 - Specifically 

Regulated Carcinogens: 

No component of this product present 

at levels greater than or equal to 

0.1 % is identified as a carcinogen 

or potential carcinogen by ACGIH. 

No component of this product present 

at levels greater than or equal to 

0.1% is identified as probable, 

possible or confirmed human 

carcinogen by IARC. 

No component of this product present 

at levels greater than or equal to 

0.1% is identified as a known or 

anticipated carcinogen by NTP. 

No component of this product present 

at levels greater than or equal to 

0.1 % is identified as a carcinogen 

or potential carcinogen by OSHA. 

This product does not contain any 

chemicals known to State of 

California to cause cancer, birth 

defects, or any other reproductive 

harm. 

No components of this product are 

subject to the SARA Section 302 

(40 CFR 302.4) reporting 

requirements. 

No components of this product are 

subject to the SARA Section 302 

(40 CFR 355) reporting 

requirements. 

No components of this product are 

subject to the SARA Section 313 

(40 CFR 372.65) reporting 

requirements. 

No components found on the California 

Director's List of Hazardous 

Substances. 

No components found on the California 

Specifically Regulated 

Attachment 6, Page 49 
Sanitized Version 

Material Safety Data Sheet 

Revision Date: 11/15/2010 

000000033729Nersion: 1.1 Kodak 

Print Date: 12/01/2010 

u.s. - California - 8 CCR Section 5203 Carcinogens: 

U.S. - California - 8 CCR Section 5209 Carcinogens: 

U.S. - Massachusetts - General Law Chapter 111 F (MGL c 

111 F) - Hazardous Substances Disclosure by 

Employers (a.k.a. Right to Know Law): 

U.S. - Minnesota Employee Right-to-Know (5206.0400, 

Subpart 5. List of Hazardous Substances): 

U.S. - New Jersey - Worker and Community Right to Know 

Act (N.J.S.A. 34:5A-1): 

U.S. - Pennsylvania - Part XIII. Worker and Community 

Right-to-Know Act (Chapters 301-323): 

U.S. - Rhode Island - Title 28 Labor and Labor Relations 

(Chapters 28-21 Hazardous Substance Right-to-Know 

Act): 

Carcinogens List. 

No components found on the California 

Section 5203 Carcinogens List. 

No components found on the California 

Section 5209 Carcinogens List. 

No components regulated under the 

Massachusetts Hazardous 

Substances Disclosure by 

Employers Law. 

No components found on the 

Minnesota Employee Right-toKnow List of Hazardous 

Substances. 

No components regulated under the 

New Jersey Worker and 

Community Right-to-Know Act. 

S 05071R Dye 

No components regulated under the 

Rhode Island Hazardous 

Substance Right-to-Know Act. 

The data below reflects current legislative requirements whereas the product in your possession may 

carry a different version of the label depending on the date of manufacture. 

US/Canadian Label Statements: 

ORM1835 

CONTAINS: S 05071R Dye (not applicable). 

WARNING! POWDERED MATERIAL MAY FORM EXPLOSIVE OUST-AIR MIXTURES. HEAT 

SENSITIVE - CAN DECOMPOSE IF HEATED. MAY BE HARMFUL IF SWALLOWED. MAY CAUSE 

SKIN AND EYE IRRITATION. OUST MAY BE IRRITATING TO THE EYES AND RESPIRATORY 

TRACT. THE PHYSICAL-CHEMICAL AND TOXICOLOGICAL PROPERTIES OF THIS MATERIAL 

HAVE NOT BEEN FULLY INVESTIGATED. 

Store in cool place. Keep away from heat and sources of ignition. Avoid breathing dust. Avoid contact 

with eyes, skin, and clothing. Use only with adequate ventilation. Wash thoroughly after handling. FIRST 

AID: If inhaled, remove to fresh air. Any material that contacts the eye should be washed out immediately 

with water. Wash off with soap and water. Get medical attention if symptoms occur. Keep out of reach of 

children. Do not handle or use until safety precautions in Material Safety Data Sheet (MSDS) have been 

read and understood. Since emptied containers retain product residue, follow label warnings even after 

container is emptied. IN CASE OF FIRE: Water spray, Carbon dioxide (C02), Dry chemical. Use water 

spray to cool unopened containers. IN CASE OF SPILL: Shovel into suitable container for disposal. 

Avoid dust formation. Clean surface thoroughly to remove residual contamination. 

Attachment 6, Page 50 
Sanitized Version 

Material Safety Data Sheet 

Revision Date: 11/15/2010 

000000033729Nersion: 1.1 Koda 

Print Date: 12/01/2010 

The information contained herein is furnished without warranty of any kind. Users should consider these 

data only as a supplement to other information gathered by them and must make independent 

determinations of suitability and completeness of information from all sources to assure proper use and 

disposal of these materials and the safety and health of employees and customers and the protection of 

the environment. The information relating to the working solution is for guidance purposes only, and is 

based on correct mixing and use of the product according to instructions. 

Attachment 6, Page 51 
ATTACHMENT HEADER SHEET 


Attachment Number 007 

Attachment Name 


Study 


Associated PMN Section Number 

N/A 

Does not contain CBI 

Report Number 


KODA101220491061974 


Page 52 
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Attachment 7, Page 68 

ATTACHMENT HEADER SHEET 


Attachment Number 008 

Attachment Name 


Analysis 


Associated PMN Section Number 

N/A 

Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 69 
Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

LC/MS ANALYSIS REPORT 

MATERIALS SEPARATION AND STRUCTURE CHEMICAL CHARACTERIZA nON LABORATORY 

LC/MS Analyst: Robert Saccente 

TECHNICAL SOLUTIONS DIVISION/ANALYTICAL SCIENCE DIVISION 

8-82 ROOM 232C 

Sample Description OneLims # MS-12B11. 

CJNI0091357WK (mw = 625 Oa) 

One sample labeled CIN10091357WK DEV91357 R3. 

Information Desired: Characterize sample by mass spectrometry. 

Experimental 

The sample was dissolved in 1:1 v:v acetonitrile: HPLC-grade water and analyzed on a Sciex AP300 triple 

quadrupole mass spectrometer using electrospray (Turbolon Spray) LC/MS in positive ionization mode. The 

primary "N solvent was 0.1 M ammonium acetate + 0.1 M acetic acid buffer, pH 4.7 in HPLC-grade water. The 

secondary "S" solvent was a 1:1 v:v mixture of acetonitrile:2-propanol. The analyses were generated using 

gradient conditions (75/25-0/100 in 20 minutes) at a flow rate of 0.25 mUmin. The reversed-phase HPLC 

column used was a Zorbax Eclipse XDS C-18 [2.1 mm X 150 mm]; 5um particle size. UV detection was 

performed using a diode array detector set at 254 nm. 

Results 

The sample responded favorably in positive ion electrospray. The LC/MS chromatogram of the sample is 

shown in Figure 1. The molecular weights of the components that responded to the mass spectrometer are 

listed in the attached sample peak table. The MS/MS spectrum of the major component is shown in Figure 2. 

HSE-Chemical & EquIpment Regulatory Intonnation 

v 4.0 

Attachment 8, Page 70 

10115101 
Sanitized Version 

EASTMAN KODAK COMPANY 


RESTRICTED INFORMATION 


Figure 1, LC/MS Chromatogram of Sample Labeled CIN1 0091357WK DEV91357 R3 at 254 nm. 

LC/MS Chromatogram of CIN10091357WK DEV91357 R3 at 254 nm. 

a Nov 2010 

!o~rectoT C~~m.nt· UV signal] 

10091357w1< dev91357·r3.esp 

[ J 
 / 

1 


3596 6601 


W W 4830.89 1327 57.21 I......J U '--..J j " iii " , 'I ' " , I I " j I • , , i I •• Ii I • I , i i ••• 'I • Ii iii " I Iii i ) I I • Ii I • , i , I " " I " i , I " i , I " " I • , " I " , • i •• Ii 


o 2 4 6 8 10 12 14 16 18 


Retention Time (min) 

HSE·Chemical & EquIpment Regulatory Information 


1011 5iO I 


v 4.0 


Attachment 8, Page 71 

Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMA TION 

Figure 2. Positive Ion Electrospray MS/MS Spectrum of Major Component (mw 625 Da) 

Positive Ion Electrospray MS/MS Spectrum of the Major Component (mw =625 Da). 8NO'Y:i:J1Q 

P , " , ., i ! , ! 

321" 

I 

, 1 I 1 , r • ,I Ii, I , I ,. j , ! ii, I j" i I ,ii ii, j I I" iiiIi' ij'

160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 62C 

rM. 

Analyst: Robert Saccente x-79577 Name: F. Debellis 

Bldg: 337/2 KP 

Phone: x-83450 

Reported________ Date: 11-08-10 Charge No. 

This report is intended for internal Kodak unrestricted use. This report may not be duplicated except in 

its entirety to preserve data integrity. The analysis is for the sample received and may not represent 

population from which the sample was taken. 

lISE-Chemical & Equipment Regulatory Information 

IOil5/01 

v 4.0 

Attachment 8, Page 72 
ATTACHMENT HEADER SHEET 


Attachment Number 009 

Attachment Name 


FT-IR Analysis 


Associated PMN Section Number 

N/A 

Does not contain CBI 

Report Number 


KODA101220491061974 


Page 73 
Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

FT -IR Spectra 

2 mg of test substance added to KBr 

Spectra acquired using a Nicolet Nexus 470 spectrometer (EPP: ICAL-SOP-0190 and SOP: lCALSOP-O 130) 

FT-IR Spectrum - Full Scale 

44~ 

42~ 

40~ II 38~ 

36~ 

34~ 

32: 

30~ 

28~ 

.... 

;Ii 26~ 

24: 

22: 

20~ 

18~ 

16: 

14~ 

12~ 

3000 2000 1&10 1000 800 600

w.venlJTtJ9($ (cm-1) 

HSE-Chemical & Equipment Regulatory Information 

10/15/0 I 

v 4.0 

Attachment 9, Page 74 
ATTACHMENT HEADER SHEET 


Attachment Number 010 

Attachment Name 


Proton NMR Analysis 


Associated PMN Section Number 


N/A 


Does not contain CSI 

Report Number 


KODA 101220491061974 


Page 75 
Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

HI NMR Spectra 

30 mg of test substance added to ImL ofOMSO-d6 in a 5mm 1.0. tube. 


Spectra acquired using a Varian Mercury-Vx 300 MHz spectrometer (QOO# A TO/EP-OP-OO 1) 


Spectra: 


I Number Type Full Expanded I Temperature Chemical 

eH, IH/IH, 13C, DzO exchange) ! COC) Shifts 

I 1 HI X I RJT 

i 2 HI X I RJT j 

FT-IR 

2.1. Results 

The spectra are consistent with structure for eIN 10091357. 

Analyst Signature: Bill Lewis Oate: November 9, 2010 

FPEG WW IC ROCH QUALITY/ANALYTICAL 

HSE-Chemical & Equipment Regulatory Information 

v 4.0 

Attachment 10, Page 76 

10/15/01 
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EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

HI NMR - Full Scale 

:1PHASEFIL.1R 

0.1 

0.09 

1 

0.08 

0.07 

0.04 

-::

:: 

·DMSO-d6 ..... 

0.03 

0.02 

0.01 


0 


6.03 3.69 14.20 

L.........J W L...---I 

10 9 8 7 654 

Olerricai Shift (ppm) 

3 2 o 

HI NMR Expanded Scale 

0050 lPHASEFlU R 

0.045 

0.040 

0.005 

o 

2.02 1.99 

9.0 8.5 8.0 7.0 6.5 6.0 

HSE-Chemical & Equipment Regulatory Information 

10115101 

v4.0 

13.37 

7.5 

Chemical Shift (ppm) 

Attachment 10, Page 77 
ATTACHMENT HEADER SHEET 


Attachment Number 011 

Attachment Name 


Particle Size Analysis 


Associated PMN Section Number 


N/A 


Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 78 
Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

Particle Size Analysis 

Particle Size (Horiba) Analyst: Gary Slater/Valerie Turner 

--+-tJ09t357R3_ns 11/09/2010 

-+-1J09t357R3_nsr 

1lO9t357R3_s 

6 

5 

4 

0.1 10 100 1000 

um 

ns=no sonicl s=sonicl r=replicate 

Filename Median 


10091357R3_ns 59.259 


10091357R3_nsr 57.677 


10091357R3_s 44.980 


10091357R3_sr 45.2054 


Filename 10091357R3_ns 10091357R3_nsr 10091357R3_s 10091357R3_sr 

10# 2.01E+14 2.01E+14 2.01E+14 2.01E+14 

Circulation 

Speed 3 3 3 3 

Ultra sonic OFF OFF 01 :00 (7) 01:00 (7) 

LaserT% 76.8(%) 76.9(%) 73.0(%) 73.5(%) 

Lamp T% 83.0(%) 82.6(%) 79.7(%) 80.0(%) 

Form of 

Distribution Standard Standard Standard Standard 

Distribution 

Base Volume Volume Volume Volume 

R.R.lndex 120a0101 120a0101 120a0101 120a0101 

HSE-Chemical & Equipment Regulatory Information 

v4.0 

Attachment 11, Page 79 

10115101 
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EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

S.P. Area 1317.6(cm2/cm3) 1359.8(cm2/cm3) 1800.5(cm2/cm3) 1775.0(cm2/cm3) 

Median 59.2589(\-Im) 57.6774(\-Im) 44.9804(\-Im) 45.2054(\-Im) 

Mean 74.0556(\-Im) 72.2779(\-Im) 58.6210(\-Im) 58.5128(l..Im) 

Variance 3229.5(l..Im2) 3160.5(l..Im2) 2609.7(l..Im2) 2530.1 (I..Im2) 

S.D. 56.8287(l..Im) 56.2182(l..Im) 51.0849(l..Im) 50.3003(l..Im) 

CV 76.7378 77.7807 87.1442 85.9646 

Mode 62.9943(\-Im) 55.2492(l..Im) 48.0797(l..Im) 48.0906(l..Im) 

Diameter Frequency (%) Frequency (%) Frequency (%) Frequency (%) 

0.022 0 0 0 0 

0.026 0 0 0 0 

0.029 0 0 a 0 

0.034 a a a a 

0.039 0 0 a a 

0.044 0 a 0 a 

0.051 a a a a 

0.058 a a 0 a 

0.067 0 a a a 

0.076 a a a a 

0.087 a a a a 

0.100 a 0 a a 

0.115 a a a a 

0.131 a 0 0 0 

0.150 0 0 0 0 

0.172 0 0 0 a 

0.197 0 0 a 0 

0.226 0 a 0 0 

0.259 0 0 0 0 

0.296 0 0 0 0 

0.339 0 0 0 a 

0.389 a a 0 0 

0.445 a a 0 0 

0.510 a 0 a 0 

0.584 0 0 a 0 

0.669 a 0 0 0 

0.766 a 0 a 0 

0.877 a 0 0 a 

1.005 0 0 0 a 

1.151 0 0 0 a 

1.318 0 0 0 0 

1.51 0 0 0 0 

1.729 0 0 0 a 

1.981 0 a 0 0 

2.269 a 0 0 0 

2.599 0 a 0 0 

2.976 0 0 a 0 

3.409 0 0 a 0 

3.905 0 a 0 0 

4.472 0 0 0 0 

5.122 0 0 0.102 a 

5.867 0 0 0.156 0.141 

HSE-Chemical & Equipment Regulatory Information 

v4.0 

Attachment 11, Page 80 

10115101 
6.720 0 

7.697 0 

8.816 0.188 

10.097 0.364 

11.565 0.617 

13.246 0.932 

15.172 1.282 

17.377 1.653 

19.904 2.064 

22.797 2.556 

26.111 3.175 

29.907 3.949 

34.255 4.864 

39.234 5.844 

44.938 6.769 

51.471 7.502 

58.953 7.935 

67.523 8.016 

77.339 7.75 

88.583 7.171 

101.460 6.342 

116.210 5.356 

133.103 4.352 

152.453 3.352 

174.616 2.503 

200.000 1.822 

229.075 1.286 

262.376 0.867 

300.518 0.563 

344.206 0.364 

394.244 0.245 

451.556 0.177 

517.200 0.138 

592.387 0 

678.504 0 

777.141 0 

890.116 0 

1019.515 0 

1167.725 0 

1337.481 0 

1531.914 0 

1754.613 0 

2000.000 0 

0 

0.156 

0.273 

0.456 

0.7 

0.99 

1.308 

1.653 

2.049 

2.543 

3.185 

4.007 

4.992 

6.05 

7.031 

7.771 

8.149 

8.128 

7.737 

7.039 

6.119 

5.083 

4.063 

3.088 

2.291 

1.669 

1.188 

0.814 

0.541 

0.359 

0.246 

0.18 

0.141 

0 

0 

0 

0 

0 

0 

0 

0 

0 

0 

0.246 

0.399 

0.636 

0.981 

1.437 

1.97 

2.525 

3.069 

3.608 

4.177 

4.811 

5.51 

6.219 

6.84 

7.261 

7.4 

7.23 

6.781 

6.114 

5.294 

4.393 

3.494 

2.673 

1.954 

1.412 

1.017 

0.73 

0.515 

0.358 

0.25 

0.182 

0.141 

0.115 

0 

0 

0 

0 

0 

0 

0 

0 

0 

0 

Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

0.226 

0.371 

0.6 

0.941 

1.395 

1.932 

2.494 

3.045 

3.588 

4.162 

4.803 

5.514 

6.242 

6.887 

7.332 

7.49 

7.326 

6.869 

6.184 

5.343 

4.422 

3.506 

2.675 

1.948 

1.397 

0.994 

0.703 

0.488 

0.335 

0.234 

0.171 

0.134 

0.11 

0 

0 

0 

0 

0 

0 

0 

0 

0 

0 

HSE-Chemical & Equipment Regulatory Infonnation 

v 4.0 

Attachment 11, Page 81 

10115101 
Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

165634 

::!010-1070 

Sample: 2010-1070 155634 F .... : 20101070.001 

Size: 1.0000 mg DSC Operator: M. Spacher 

Memo<!: 25-420C GI 1 DC/mIn N2 Run Dale: 28-Sep-2010 08:24 

7~------------------------------------------------------------------------~ 

4 

2 

328. t'e 

Temperature ('C) 

Total En~l"IY (J/I;) 545 


Rate (WtV J.lili 


dT!dt (Clmio.) 261 

R*E IH!: 

Slope ofMaximmn 

Peak (\\iIg/C) 

Wulih al Hilf 

He1ghl(C) 

DSCCel1 850 

Endo ExtrapolatM 

Onset Temp (C) 

Em Onset Temp (C) 

Em EitrapolatM 

Onset Temp (C) 

THDp at Maximu. 

P~ak(Tmhr. C) 

Residue DeSaiptiOll 

94 

132 

185 

198 

black 

Cell Sase 2920 

10119:2010 

HSE-Chemical & Equipment Regulatory Information 

1011510 I 

v4.0 

Attachment 11, Page 82 
i 

Sanitized Version 

· PART IV -- Certification Summa~ryL...-__ -----~------~--~----___1 

The iriformation provided is accurate to the best ofour abilities and knowledge. The sample 

analyzed and discussed is representative ofwhat will be manufactured/importedfor commercial 

purposes. The analytical methods discussed are appropriate for the type andform ofthe chemical 

substance and the analytical data has been interpreted and confirms the composition. The 

components that function as the intended use have all been identified 

Date completed: 11-09-201 0 

: Commercialization team members involved with completing the Certificate ofComposition 

rJiSErepresentative:Judy VanNorstrand m •• ..--------4

i Commercialization Team Leader:James Matz 

Process chemist:Stephen Wark 

: Development chemist:John Harder/Fran Debellis 

I Polymer chemist: ------------­

i-­ chemist: W. . G. Sl-a-te-r-IV.-.-r,-u-r-n-er-------------------j 

HSE-Chemical & Equipment Regulatory Information 

v 4.0 

Attachment ii, Page 83 

10/15101 
Sanitized Version 

Instructions for Certificate of Composition 

For the purpose of documenting the correct identity of a chemical when HSE testing and/or a new chemical 

notification is needed. This form is to be completed during RTDP/KMCP by appropriate commercialization team 

members. 

It is intended that members of a commercialization team complete this form after, 

);­ a representative sample of the chemical is selected 

);­ the representative sample is analyzed with the appropriate methods to determine the composition 

);­ the analytical data are interpreted 

);­ the components that function as the intended use have been identified 

The completed form is returned to the HSE representative on the commercialization team. 

Instructions: 

1) Chemical Substance: IdentifY the chemical using the internal name and/or the CIN if a mixture. For an intermediate identifY 

the final (i.e. intermediate for the dye ...). 

2) Part I: For a non-polymer, 

a. 	 List the components that are present in a representative sample ofthe chemical. Include isomers, tautomers, 

stereo isomers, analogs, impurities, and solvents. For each componcnt provide, 

i. KAN and/or ClN 

ii. 	 Name (Attach Structure) 

iii. 	 Range of percent in representative sample 

iv. 	 A chemical is notified with a commercial purpose. (i.e. dye, sensitizer, antifoggant, intermediate ...) All 

components in the sample that functions as the intended purpose must be identified. Provide yes or no 

under the column, Functions as Intended Use? 

b. 	 For imported chemicals, use the Certificate of Analysis, MSDS, and/or any other data provided by the vendor to list 

the components in Part I. If Kodak analyzed the imported chemical, confirm Kodak's analytical data is consistent 

with the information provided by the vendor. 

3) 	 Part II: For a polymer, 

a. 	 List the monomers and reactants that make up the polymer. For each monomer and reactant provide: 

i. KAN/CIN 

ii. 	 Name (Attach Structure) 

iii. 	 Weight percent in the polymer 

iv. 	 Maximum weight percent as residual 

b. 	 Give the lowest number average molecular weight 

c. 	 Maximum weight percent below 500 molecular weight 

d. 	 Maximum weight percent below 1000 molecular weight 

e. 	 Attach structure of polymer and synthetic pathway (include initiators and non-isolated intermediates). 

f. 	 For imported chemicals use the Certificate of Analysis, MSDS, and/or any other data provided by the vendor to 

complete Part II. If Kodak analyzed the imported polymer, confirm Kodak's analytical data is consistent with the 

information provided by the vendor. 

4) 	 Part III: Analytical Methods 

a. 	 Briefly summarize the analytical methods and results used to confirm the composition ofthe representative sample. 

(Analytical methods may include, literature examples, vendor information, professional interpretation when necessary) 

b. 	 Provide the name and organization of the person who provided the analytical summary. 

c. 	 IfKodak analyzed the imported chemical, confirm Kodak's analytical data is consistent with the information 

provided by the vendor. 

d. 	 When a chemical is notified all physico-chemical data in our possession must be submitted. Please attach one 

example for each type of analytical method obtained. (i.e. If4 NMR's were done on the representative sample just 

provide one of the four) Please include all Vendor information pertaining to an imported chemical. 

5) 	 Part IV: Certification Summary 

a. 	 Provide the date the team members agree that the Certificate of Composition is complete and "The information 

provided is accurate to the best of our abilities and knowledge. The sample analyzed and discussed is representative 

of what will be manufactured/imported for commercial purposes. The analytical methods discussed are appropriate 

for the type and form of the chemical substance and the analytical data has been interpreted and confirms the 

composition. The components that function as the intended use have all been identified." 

b. 	 The commercialization team members involved with the completion of the Certificate of Composition sign part IV. 

HSE-Chemical & EqUipment Regulatory Information 

v 4.0 

Attachment 11, Page 84 

10/15/01 
ATTACHMENT HEADER SHEET 


Attachment Number 012 

Attachment Name 


DSC Analysis 


Associated PMN Section Number 


N/A 


Does not contain CBI 

Report Number 


KODA101220491061974 


Page 85 
Sanitized Version 

EASTMAN KODAK COMPANY 

RESTRICTED INFORMATION 

DSC Analysis 

165634 

1010-1070 

Sample: 2010-11)70 165634 File: 20101070.001 

Size: 1.0000 II'Ii:I DSC Operator; M. Spacher 

Method: 25-420C 01 1 OClmln N2 Run OablO 28-Sep-20 1 0 06:24 

7~---------------------------------------------------------------------, 

6 


5 


4 


o 

-1 

-2 

Temperatu.... ("C) 

Total Energy (JIll 545 


RatelWig5 Hili 


dT/dt (Clmin) 261 

Ri;E I!JJ!: 

Slope ofMaximum 

Pe"u (WigIC) 

Wulih at BBlf 

Height(C) 

DSCCell 850 

Endo Extrapolated 

Onset Temp (C) 

Exo Onset Temp (C) 

Exo Extrapolated 

Onset Temp (C) 

Tt'1Dp atMasiJmuJl 

Peak (TDibr. C) 

94 

132 

185 

198 

Residue DeScripriOll black 


CeJIBase 2920 


10/19/2010 

HSE-Chemical & Equipment Regulatory Information 

10/15/0] 

v4.0 

Attachment 12, Page 86 
ATTACHMENT HEADER SHEET 


Attachment Number 013 

Attachment Name 


Chemical Safety Data Sheet 


Associated PMN Section Number 


N/A 


Does not contain CSt 

Report Number 


KODA101220491061974 


Page 87 
TSL }'ILE ID 165634 Chemical Safety Data Sheet (CSDS) 

Empty Data Fields Indicate That NO Data Were Available 

Date Printed: ] ]/]2/2010 Technical Safety Laboratory, B-320 Room 2103 Phone Ext. 26604 for Inquiries PAGE 1 OF 2 Release Versioll9 OIS\\1NT12032004 

. TESTING IN PROGRESS 

Molecular Weight: 

Mol. Weight: 661 Approval Date 06/1512010 

Solubility 

FLAMMABILITY 

i 

o 

=r 

3 

CD 

::I

.... 

...10 

~ FLAMMABLE LIMITS (EXPLOSIVE LIMITS) 

"'0 

I» 

CO 

CD 

C» 

C» 

THERMAL STABILITY 

Test Atmosphere Pan Type Tcst Pressure (psia) Exo To (C) Tmpr dP/dt Total Energy (JIg) Heating Rate Sample Weight Tmhr Rate dTfdt ROE Max Scan Temp (C) 

IARITsoage llItrogen Hastelloy-C 3.00 91.00 

STABLE AT 90(' FOR 72 fl( (IRS. 

IDse SCTeen* llItTogen glass capillary I)u j4'J 10 C/min 100 200 3.28 234 1145 

IIJSC Screen mtmgen gtass capillary U2 )4~ 10 e/min 1.00 198 3.66 261 1995 

IResidue DSC 

!ARC ISOAG 

mtTogen 

AT90C FOR 7 

glass capillary 

HOURS 

159 498 10 C/min LaO 197 2.99 214 1489 

IVSP llItrogcn Upen vent, 114, s 170 196 27 40.00 225 1388 

en 

I» 

::I 

;::;: 

N' 

CD 

Q. 

~ 

U1O· 

HAZARD RATINGS 

Heat Sensitivity Code (H) Y Impact Sensitivity Code (I) 0 'lnermal Energy Code (T) 2 Peroxide Fonner Code (El o Other Reactivity Code (0) 0 

Water Reactivity Code (W) 0 Polymerizability Code (Z) 0 Oxidi7.er Code (X) o Pyrophoric Code (Al N 

::I 
TSL FILE ID 165634 Chemical Safety Data Sheet (CSDS) 

Rmpty Data Fields Indicate That NO Data Were Available 

Date Printed: 11112/2010 Technical Safety Laboratory, B-320 Room 2103 Phone Ext. 26604 for Inquiries 

TESTING IN PROGRESS 

REACTIVITY 

Incompatibility Strong oxidiz€'r 

Incompatibility Other 


Polymerization' 


Process Review Comment" 


Heat of Poly (JIg): 

Peroxide #: 

Peroxide Statement: 

» Miscellaneous Infol]!!1!!;<:lIl:

6f 

(") Incident: 
 :T 


3 

~ Incident Comment: :::I 

..... 

~ References: ~ 

~ CO Summary: ~ 

CO 

<0 

Other Comments: 

Peroxide Hrs: Peroxide Temp: 

Deflagration and Detonation Potential: en 

Dl 

:::I 

;::;: 

ii:j' 

& 

~ iil O· 

:::I 
ATTACHMENT HEADER SHEET 


Attachment Number 014 

Attachment Name 


Acute Toxicity to Zebra Fish S0507 


Associated PMN Section Number 


N/A 


Does not contain CBI 

Report Number 


KODA101220491061974 


Page 90 
Sanitized Version 

Environmental Assessment and Pollution No.: R2010NC037·01 

SPONSOR 


Intertek(Tianjin) Testing Service Ltd Shanghai Branch 


REPORT FOR 


REPORT OF ACUTE TOXICITY TO ZEBRA FISH (BRACHYDANIO RERIO) 


TEST SUBSTANCE: S0507 


Study No.: S2010NC037-01 


Report No.: R2010NC037-01 


Study Director: Liu Jining 


Date of Report Completion 


Dec. 7, 2010 


{~\ Key Lab of Pesticide Environmental Assessment and Pollution Control, MEP 

Address: 8 Jiang-wang-miao Street, Nan/jing 210042, China 

Tel: (+86)2585287074 Fax: (+86) 25 85474630 

Page I of28 

Attachment 14, Page 91 
Sanitized Version 

Lab of Pesticide Environmental and Pollution No, R2010NC037-01

CONTENTS 


SPONSOR AND TEST FACILITY ......................................................................................................... 4 


STATEMENT OF GLP COMPLIANCE ................................................................................................. 5 


QUALITY ASSURANCE STATEMENT............................................................................................... 6 


STUDY DETAILS PAGE ......................................................................................................................... 7 


I SUMMARy............................................................................................................................................ 8 


2 INTRODUCTION ................................................................................................................................ 10 


3 EQUIPMENT & MATERIALS........................................................................................................... 11 


3.1 Date of Test ......................................................................................................................... II 


3.2 Test Organism ..................................................................................................................... 11 


3.3 Dilution Medium ................................................................................................................. 11 


3.4 Apparatus............................................................................................................................. 12 


3.5 Reference Substance ............................................................................................................ 12 


4 TEST DESIGN ..................................................................................................................................... 12 


4.1 Preparation of the Test Solutions ......................................................................................... 12 


4.2 Observations and Evaluations ............................................................................................. 13 


4.3 Range-finding Test .............................................................................................................. 13 


4.4 Definitive Test ..................................................................................................................... 14 


4.5 Validity of Test.................................................................................................................... 15 


4.6 Stability of Test Solution and Chemical Analysis ............................................................... 15 


5 DATA PROCESSING ......................................................................................................................... 17 


6 RESULTS ............................................................................................................................................. 18 


6.1 Analytical Method for Determination of S0507 in Water ................................................... 18 


6.2 Analysis of S0507 in Test Solutions .................................................................................... 18 


6.3 Test Condition ..................................................................................................................... 18 


6.4 Mortality and Effects ........................................................................................................... 19 


6.5 Conclusions ......................................................................................................................... 19 


7 HEALTH & SAFETY .......................................................................................................................... 19 


8 MAINTENANCE OF RECORDS & DOCUMENTATION ............................................................. 20 


Page 2 of28 

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9 REFERENCE ................................ _...................................................................................................... 20 


TABLES ................................................................................................................................................... 21 


Table I Recovery ofS0507 in Test Medium ............................................................................. 21 


Table 2 Stability Test Results of S0507 in Test Medium (Definitive Test) .............................. 21 


Table 3 Water Quality Parameters of Test Solutions during the Definitive Test.. .................... 22 


Table 4 Toxicity of Potassium Dichromate to Brachydanio rerio ............................................ 23 


Table 5 Mortality during the Range-finding Test.. .................................................................... 23 


Table 6 Mortality during the Definitive Test.. ........................................................................... 24 


Table 7 Visual Observations during Definitive Test.. ............................................................... 25 


Table 8 Characteristics of the Dilution Water ........................................................................... 26 


FIGURE ................................................................................................................................................... 27 


Figure I Calibration Curve for the Test Item ............................................................................ 27 


Figure 2 Standard UPLC-MS/MS Chromatogram ofthe Test Item (lmg/L)............................ 27 


Figure 3 UPLC-MS/MS Chromatogram of the Recovery Test Sample (0.1 mg/L) .................. 27 


Figure 4 UPLC-MS/MS Chromatogram of the Control Sample (Definitive Test) ................... 27 


Figure 5 UPLC-MS/MS Chromatograms ofthe Test Item in Definitive Test (1.6 mg/L) ........ 28 


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Lab of Pesticide Environmental No.: R201ONC037·01 

SPONSOR AND TEST FACILITY 

Sponsor: Intertek(Tianjin) Testing Service Ltd Shanghai Branch 

Address: 2F,Building No.4 Shanghai Comalong Industrial Park, 889 Yi Shan Road, 

Shanghai, China, 200233 


Contact person: Li Xangjun 


Tel: 02160915013 


Test Substance: S0507 

Test Facility: Key Laboratory of Pesticide Environmental Assessment 

and Pollution Control, MEP (PEAPC) 

Address: 8 liang-wang-miao Street, Nanjing 210042, China 

Tel.: (+86) 25 85287074 

Fax: (+86) 2585474630 

Legal Representative Person: Gao Zhenning, Director-general, professor 

Laboratory Management: Shan Zhengjun, professor 

Head of Department: Shi Lili, professor 

Study Director: Liu Jining 

Study Personnel: Wang Lei, Tian Jing, Wu Guanqun 

Quality Assurance: Ge Feng 

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Lab of Pesticide Environmental Assessmenl and Pollution No.: R2010NC037-0J 

STATEMENT OF GLP COMPLIANCE 

Study No.: S2010NC037-01 

Report No.: R20 1 ONC037 -01 

According to "OECD Guidelines for testing of chemicals", "The guidelines for the 

testing of chemical (HJ/T 153-2004)"and "The guidelines of chemical testing good 

laboratory practices (HJ/T 155-2004)" issued by State Environmental Protection 

Administration (SEPA) of the People's Republic of China, this experiment was 

conducted under CMA (China Metrology Accreditation) and CNAS (China National 

Accreditation Service for Conformity Assessment) experimental conditions at our 

laboratory. The experimental protocol was strictly carried out in the process of the 

experiment, and the present report has reflected the experimental results truly and 

correctly. 

(Study Director) Date: 

(Laboratory Management) Date: 

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QUALITY ASSURANCE STATEMENT 


Study No.: S2010NC037-01 

Report No.: R20IONC037-01 

This experiment was carried out in accordance with the experimental protocol. It is 

hereby certified that what the present report describes has accurately reflected the raw 

data of the experiment. 

During the on-site process, QAU Inspections were perfonned for this study. The dates of 

Quality Assurance inspection are given below. 

inspections 

Phase/Process 

Inspection Reporting 

Protocol Sept 10, 2010 Sept 10, 2010 

Study Draft Report Dec. 2, 2010 Dec. 2, 2010 

Test solutions preparation Oct. 4, 2010 Oct. 4, 2010 

Process Observation/Determination Oct. 8,20 

(Person responsible for QAU) Date: 

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Lab of Pesticide Assessment and Pollution Control. MEP No. R2010NC037-0 I 

STUDY DETAILS PAGE 

Study number: 


Report number: 


Study title: 


Test substance: 


Identity: 


Chemical Name: 


Molecular formula: 


Molecular weight: 


Appearance: 


Storage conditions: 


Management: 


Head of Department: 


Study Director: 


Person attending to routine 

duties and technical queries in 

the temporary absence ofthe 

Study Director: 

Study Director contact details: 

Location of study: 

Study dates 


Start: 


Completion: 


Draft report: 


------..--.. 

S20lONC037-01 

R201ONC037-01 

Acute Toxicity to Zebra fish (Brachydanio rerio) 

S0507 

S0507 

3H-lndolium, 2-[2-[3-[( I ,3-dihydro-I ,3,3-trimethyl-2H-indol-2­

y lidene )ethy Iidene ]-2-[( 1 -phenyl-I H-tetrazol-5-yl)thio ]-1 

cyclohexen-I-yl]ethenyl]-l ,3,3-trimethyl-, chloride 

C39H4IN6S'CI 

661.31 

Solid 

Room temperature 

Shan Zhengjun 

Shi Lili 

Liu lining 

Wang Lei 

Liu lining 

Telephone: +862585287091 

Facsimile: +8625 85474630 

Email:lin@nies.org 

Key Lab of Pesticide Environmental Assessment and Pollution 

Control, MEP 

8 liang-wang-miao Street 

Nanjing 210042, liangsu, China 

Oct. 4, 2010 

Nov. 19,2010 

Dec. 2, 2010 

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1 SUMMARY 

Under Semi-static conditions (24h-renewal), the acute toxicity of test item (S0507) to 

zebra fish (Brachydanio rerio) was conducted according to the following guidelines: 

"The guidelines for the testing of chemicals", SEPA (HJ/T 153-2004); Procedure 203 of 

the "Guidelines for Testing of Chemicals" of the OECO: "Fish, Acute Toxicity Test" 

(1992) etc. 

During the whole test period, the pH values of the control mediums and test mediums 

were between 7_36 and 756, and the Dissolved Oxygen (~O) values varied from 101% 

to 108% of the air saturation at the test temperature, and the total hardness was in the 

range of 119 mg (CaCO])/L to 125 mg (CaC03)/L. During the test, the temperature of 

the test mediums were maintained in the range of23.2°C to 23SC, and all fishes in the 

control group were normal. With the same conditions, K2Cr207 was used as the negative 

control substance, and the resulting 24 h-LCso was 282 mglL. So the study met the 

acceptability criteria prescribed by the protocol (pH: 6.0~8.5; dissolved oxygen 

concentration: > 60% of the air saturation value; total hardness: 1O~250 mg (CaCO])/L; 

temperature: (23 :::2) DC; 24 h-LCso of K2CrZ07 in the range of 200 to 400 mg/L). 

Therefore the test was considered valid. 

In order to confirm the stability of the test item in the test medium, concentrations of the 

test samples during the Definitive Test were analyzed by UPLC-MS/MS. A linear 

regression equation was obtained with the peak area response (A) vs. the concentration 

of the test item (c, mg/L): A =30811 c -344.13, with good linearity of rJ=0.9992. The 

results show that linearity for the concentration range of 0.01 mg/L to 2 mglL is good. 

Besides, the minimum detection amount ofUPLC-MS/MS for S0507 is 5x IO-"g, and the 

minimum detection concentration for water sample is 0.01 mglL. The analytical results 

showed that the concentration of the test item is consistent in the test medium throughout 

the 96-hour test period (deviation within 20%). Thus a Semi-static procedure 

(24h-renewal) was reasonable. 

The results showed that under valid Semi-static test conditions (24 h-renewal), the 96 

h-LCso of the test item to zebra fish is 0.78 mglL (analysed concentration 0.71 mglL), 

with 95% confidence limit of 0.71 mg/L to 0.85 mg/L. The maximum tested 

concentration causing no mortality (NOEC) is 0.2 mglL (analysed concentration O. 191 

mglL). The lowest tested concentration at which the substance is observed to have a 

lethal effect (LOEC) is 0.4 mglL (analysed concentration 0.389 mg/L). The minimum 

concentration causing 100% mortality was 1.6 mglL (analysed concentration 1.37 mglL). 

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According to "The guidelines for the hazard evaluation of new chemical substances 

(HJ/T 154-2004)", S0507 belongs to the category of very toxic chemical for zebra fish 

(Brachydanio redo). 

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2 INTRODUCTION 

This test was designed to determine the acute toxicity of test item (S0507) to zebra fish 

(Brachydanio rerio). The test fishes were exposed for 96 hours to the test solution. The 

study comprised at least one range-finding test followed by a definitive test to determine, 

where possible, the 96-hour median lethal concentration (LCso) of the test item, with 

95% confidence limits. Where no toxicity was seen in the range-finding test, it would be 

followed by a Limit Test at a single concentration. 

The study met the requirements of: 

[1] 	 "The guidelines for the testing of chemicals", SEPA (HJ/T 153-2004). 

[2] 	 HJ/T 154-2004.The guidelines for the hazard evaluation of new chemical substances. 

SEPA. 

[3] 	 "The guidelines for the testing of chemicals", Beijing: China Environmental 

Sciences Publishing House. 2004. SEPA. 

[4] 	 Part C.l (Acute Toxicity for Fish) of EC Methods for the Determination of 

Ecotoxicity; - Annex to Council Regulation (EC), L 142 (2008). 

[5] 	 Procedure 203 of the 'Guidelines for Testing of Chemicals' of the OECD: "Fish, 

Acute Toxicity Test" (1992). 

[6] 	 Ecological Effects Test Guidelines, OPPTS 850.1075, Fish Acute Toxicity Test, 

Freshwater and Marine. EPA 712-C-96-118, April 1996. 

The Guide for Care and lise of Laboratory Animals (1988) 

The in-life experimental procedures undertaken during the course of this study were 

subject to the provisions of the Guide for Care and Use of Laboratory Animals (1988) in 

China. The Guide, administered by the Ministry of Science and Technology of the 

People's Republic of China, regulates all scientific procedures in living animals which 

may cause pain, suffering, distress or lasting harm and provides for the designation of 

establishments where procedures may be undertaken, the licensing of trained individuals 

who perform the practical techniques and the issue of project licences for specified 

programmes of work. 

This study complied with all applicable sections of the Guide and the associated Codes 

of Practice for the Housing and Care of Animals used in Scientific Procedures. 

The number of animals used was the minimum that was consistent with scientific 

integrity and regulatory acceptability, consideration having been given to the welfare of 

individual animals in terms of the number and extent of procedures to be carried out on 

each animal. 

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3 EQUIPMENT & MATERIALS 

3.1 Date of Test 

Oct. 4, 2010 ~ Nov. 19,2010 

3.2 Test Organism 

The test species of Zebra fish, Brachydanio rerio, were obtained as fry from a 

commercial fish supplier of Guangzhou Hongfa Aquatic Animals Culture Company in 

China. Details concerning the source ofthe fish were recorded in the raw data. 

Feeding fry were held for 14 days in holding tanks supplied with a continuous flow of 

aerated water before being used for testing. Fish to be used in the test was held for 7 days 

in water of the quality and temperature to be used in the test. 

A photoperiod of 16 hours light, provided by overhead fluorescent tubes, and 8 hours 

dark was maintained. The oxygen concentration was about 98% of the air saturation 

value. 

The fish was fed daily during the holding period on proprietary fish food. They were 

held without food for approximately 24 hours before being placed into the test vessels. 

The ingredients of the fish food are given as follows: 

Crude Protein >49.0% 


Crude Fat> 5.0% 


Crude Fibre < 3.0% 


Crude Ash < 12.0% 


Moisture < 10.0% 


During the holding period the tanks was inspected daily and any debris or unhealthy or 


dead fish removed. 


After the 48 hour settling-in period, no mortality was observed in the following 7 days. 


So this batch of fish was accepted (Batch No. F20 10091 I). 


The wet-weight of the fish used to the test was 0.19 g in average, with an average length 


of2.3 cm. 


3.3 Dilution Medium 

Good quality tap water which had been dechlorinated for at least 24 hours was used. The 

total hardness of the test water was 122.2 mg (CaC03)/L, oxygen concentration was 

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about 98% of the air saturation value and pH was 7.55 at room temperature. 

Characteristics of the dilution water are measured at least twice a year by Jiangsu 

Provincial Center for Disease Prevention and Control. The latest measuring result is 

showed in Table 8. 

3.4 Apparatus 

Normal laboratory apparatus and: 

(a) Oxygen 	meter (HACH HQ30d. The Dissolved Oxygen can be directly read by 

immersing the probe in the water sample when the reading is stable.); 

(b) Thermometer; (HACH HQ30d. The Temperature can be directly read by immersing 

the probe in the water sample when the reading is stable.); 

(c) Equipment for determination 	of hardness of water (Model: 16900, made by HACH 

company, the hardness can be directly read by immersing the probe in the water 

sample when the reading is stable.); 

(d) Tanks made 	of chemically inert material, with a sealable inert lid, and with a 

capacity of approximately 8 L (Sanhe Zuping Glass Instrument Factory, Haimen); 

(e) Thermostatic water bath (Chang Yuan Medical Instrument Factory, Jiangsu); 

3.5 Reference Substance 

Reference substance: Potassium dichromate, K2Cr207, CAS: 7778-50-9; purity: 2':99.8%; 


Lot number: 10011460037; Nanjing Chemical Reagent Co. Ltd. 


The test with the reference substance is performed at least once a year as a means of 


assuring that the laboratory test conditions are adequate and have not changed 


significantly. The most recent test was performed in July 2010. The results of this study 


(archived under Project number S20IONCOI2) are shown in Table 4. 


4 TEST DESIGN 

4.] Preparation of the Test Solutions 

In the range-finding test, the test solutions of S0507 were prepared by directly dissolving 

appropriate amount of S0507 in dilution medium and then facilitating its dispersion by 

ultrasonication for 30 min. 

In the definitive test, stock solution of the test substance (1000 mg/L) was prepared by 

dissolving 0.1006 g S0507 into 100 mL test water. The test solutions were prepared by 

diluting the stock solution. 

Details about the Preparations are showed as follows: 

Nominal The Amount of Dilution Medium Test Type Concentration The Test Volume (L) 

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. R20 I ONC037·0 I 

(mglL) Substance Added 

(g) 


Range-finding Test 0 0 5 


0.005 5 

10 0.051 5 

100 0.502 5 

Nominal The volume of 

Dilution Medium 

Test Type Concentration The stock Solution 

Volume (L) (mglL) Added (mL) 

Definitive Test 0 0 5 

0 0 5 

0 0 5 

0.1 0.5 5 

0.1 0.5 5 

0.1 0.5 5 

0.2 5 

0.2 5 

0.2 I 5 

0.4 2 5 

0.4 2 5 

0.4 2 5 

0.8 4 5 

0.8 4 5 

0.8 4 5 

1.6 8 5 

1.6 8 5 

1.6 8 5 

4.2 Observations and Evaluations 

During the test, all kinds of abnormal responses of the fish observed would have been 

recorded, such as mortality, inactivity, strange swimming pattern, other abnormal 

behaviour, etc .. Fish would have been considered dead if there is no visible movement 

(e.g. gill movements) and if touching of the caudal peduncle produces no reaction. 

4.3 Range-finding Test 

The range-finding test, carried out under static conditions, was regularly conducted to 

determine the range of concentrations for the subsequent test. 

In the range-finding test, groups offish (5 per group) were exposed to the test solutions 

with nominal concentration of 1 mglL, 10 mglL and 100 mglL. Synchronously one 

control group was conducted in test water without the test item. For each test tank (Total 

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volume: 8 L) 5 L test solution was filled in. No replicates were used. 


The test fish were be randomly chosen and put in different test solutions after the 


temperature has been adjusted to the required value. This was done in 30 min. 


During the test, the following conditions were maintained: 


-Light: 16 hours photoperiod daily (light intensity: 1000 to 1500 lux); 


-Temperature: 23.2 °C to 23.5 DC; 


-Oxygen concentration: 90% to 102% of the air saturation; 


-Feeding: none. 

Test duration was 96 hours. Dead fish was removed at least once daily and discarded. 

The mortalities of the fish were recorded at 24-h, 48-h, 72-h and 96-h, and then the 

maximum concentration causing no mortality (NOEC) and the minimum concentration 

causing 100% mortality were determined. 

4.4 Definitive Test 

Semi-static method (24h-renewal) was adopted in Definitive Test according to the 

stability of the test solution indicated by Table 2. Based on the results of the 

range-finding test, test concentrations of 0.1 mglL, 0.2 mglL, 0.4 mg/L, 0.8 mg/L and 1.6 

mg/L were assigned in Definitive Test. Synchronously one control group was used in the 

test. Three replicates were assigned for each treatment group and control group, while 

the initial number of testing fish was 10 for each replicate. 

The test fish were be randomly chosen and put in different test solutions after the 

temperature has been adjusted to the required value. This was done in 30 min. 

During the test, the following conditions were maintained: 

-Light: 16 hours photoperiod daily (light intensity: 1000 to 1500 lux); 

-Temperature: 23.2 °C to 23.5 DC; 

-Oxygen concentration: 101 % to 108% of the air saturation; 

-Feeding: none. 

At 24-h, 48-h, 72-h and 96-h, the mortalities of the fish were recorded, and observations 

on individual behaviour were performed. Meanwhile, measurements of pH, dissolved 

oxygen and temperature were carried out and recorded daily. 

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R201OJ-:C037-0I 

4.5 Validity of Test 

4.5.1 Control group 

A control group, comprising the same number of fish as that exposed at each test 

concentration, was placed into test water alone. 

4.5.2 Reference substance test 

With the conditions maintained as before, K2Cr207 was be used as the test substance and 

the resulting 24 h-LCso was 282 mgiL The results of this study are shown in Table 4. 

4.5.3 Fish loading 

0.2 g to 0.6 g fish (wet weight) per litre of test medium. 

4.5.4 Validity of test result 

During the whole test period, the pH values of the control mediums and test mediums 

were between 7.36 and 7.56, and the Dissolved Oxygen (DO) values varied from 101% 

to 108% of the air saturation at the test temperature, and the total hardness was in the 

range of 119 mg (CaCO})/L to 125 mg (CaCO})/L During the test, the temperature of 

the test mediums were maintained in the range of 23.2°C to 23.5°C, and all fishes in the 

control group were normal. With the same conditions, K2Cr207 was used as the negative 

control substance, and the resulting 24 h-LCso was 282 mgiL So the study met the 

acceptability criteria prescribed by the protocol (pH: 6.0-8.5; dissolved oxygen 

concentration: > 60% of the air saturation value; total hardness: 10-250 mg (CaCOJ)/L; 

temperature: (23 ±2) 24 h-LCso of K2Cr207 in the range of 200 to 400 mg/L). 

Therefore the test was considered valid. 

4.6 Stability of Test Solution and Chemical Analysis 

4.6.1 Preparation of standard stock solution 

Storage solution I of the test substance (1000 mgiL) was prepared by dissolving 0.05 g 

S0507 into 50 mL methanol. 

The standard stock solution II oftest substance (10 mgiL) for the calibration curve was 

prepared by diluting 1 mL of solution I with methanol to a total volume of 100 mL. 

The standard stock solution III of test substance (0.1 mgiL) for the calibration curve was 

prepared by diluting 1 mL of solution II with methanol to a total volume of 100 mL. 

4.6.2 Working solution 

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Prepare the standard working solution of 0.0 I to 2 mg/L by drawing and diluting the 

above storage solutions with Mobile phase (Methanol: water 80:20 (v:v)). Details of 

the working solutions are showed as follows: 

The Concentration The Volume of the 

Final Volume after 

Concentration ofthe Storage Storage Solution 

Dilution 

(mglL) Solution Added Added 

(mL) (mglL) (mL) 

0.01 0.1 I ]0 

0.02 0.1 2 10 

0.05 0.1 5 10 

0.1 10 0.1 10 

0.2 10 0.2 JO 

0.5 JO 0.5 10 

10 I 10 

2 JO 2 10 

4.6.3 UPLC-MS/MS conditions 

Apparatus: ACQUITY RTM ultra Perfonnance LC (Waters, USA) 

Column: ACQUITY UPLC® BEH CI8 i.7!lm, 2.1 mmx50 mm (Waters, USA) 

Detector: Quattro Premier XE MS-MS (Waters, USA) 

Mobile phase: Methanol: water = 80:20 (v:v) (containing 0.1 % Fonnie acid) 

Column Temperature: 30°C 

Flow rate: 0.3 mLimin 

Injection volume: 5 ilL 

MS/MS detection parameters are as follows: 

Data type: MRM data 

Ionisation mode: Electrospray 

Polarity: Positive 

Capillary: 3.0 kV, Extractor: 2.0 V, RF Lens: 0.3 V 

Source Temperature: I 10°C, Desolvation Temperature: 380°C 

Cone Gas Flow: 50 Llh, Desolvation Gas Flow: 500 Lih 

LM Resolution I: 13.0, LM Resolution 2: 13.0 

HM Resolution I: 13.0, HM Resolution 2: 13.0 

Ion Energy 1: 0.4, Ion Energy 2: 2.0 

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Collision Gas (Pressure): 3.58e·3 mbar 


Parent (m/z) Daughter (m/z) Dwell (s) Cone Volt. (V) Col. Energy (eV) 


625.9 479.44 0.100 35 20 


Under the above conditions, the retention time ofS0507 was about 0.64 min (see Fig. 2). 


4.6.4 Sampling and analysis of the test solution 

Samples were taken (at least in duplicate) from each concentration at the beginning and 

for each renewal during the Definitive Test (at 0 h, 24 h(before/after renewal), 48 

h(before/after renewal), 72 h(before/after renewal) and 96 h). On each occasion, one 

sample was analysed after certain pre-treatments; the remaining samples were retained in 

case further analysis would be required. 

4.6.5 Pre-treatment method ofthe sample 

Water samples collected were filtrated by 0.22 j..lm millipore membrane and then the 

filtrates were analysed by UPLC-MS/MS. 

5 DATA PROCESSING 

Korbor processing method should be used to calculate the LCso and LC50 confidence 

limits. 

(1) LCso calculate formula 

Formula (I) 

Where, LCso-the concentration leading to 50% mortality ofthe fish (mg/L); 

Xm--Iogarithm of highest concentration; 

i-logarithm of two concentration that is next; 

I;P-sum of all treatment mortality (expressed as decimal). 

(2) LCso Calculate confidence limits (P=0.95) formula 

Formula (2) 

Where, p--mortality in one treatment; 

q-l-p; 

i-logarithm of two concentration that is next; 

n-number of fishes in every treatment. 

Confidence limits (P =0.95) of log(LCso )= log(LCso )± 1.96S log(LCso ) 

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6 RESULTS 

6.1 Analytical Method for Determination of S0507 in Water 

(I) Calibration curve 

A series of standard solutions with concentration at 0.01 mglL, 0.02 mglL, O.OS mg/L, 

0.1 mglL, 0.2 mglL, O.S mglL, I mg/L and 2 mglL were measured under the 

UPLC-MS/MS conditions mentioned above, Based on the test result, a linear regression 

equation was obtained between the concentration and the area of the peak emerged at 

0.64 min: A =30811 c -344,13, with good linearity of r

2

=0.9992, where A represents 

peak area; and c is the concentration of the test item (mglL) (See Figure 1). The results 

show that I inearity for concentration range of 0.0 I mg/L to 2 mg/L is good. 

(2) Recovery & Precision 

The recovery samples with concentrations at 0.1 mglL and 2 mglL were prepared by 

adding 0.1 mL and 2 mL of standard stock solution (10 mglL in methanol) to test water 

(IOmL) and then the solutions obtained were filtrated by 0.22 f.lm millipore membrane 

and then the filtrates were analysed by UPLC-MS/MS. 

Measurements obtained from the recovery test were shown in Table I. Figure 3 showed 

the chromatograph of the recovery sample from the recovery test. The recovery rate was 

102~ I 04% among the concentration range from 0.1 to 2 mgiL. The relative standard 

deviation was between 4.81~S.S2%. 

(3) Detection limit 

The minimum detection amount of UPLC-MS/MS for SOS07 is SxIO· llg, and the 

minimum detection concentration for water sample is 0.01 mgiL. 

6.2 Analysis of S0507 in Test Solutions 

The analyzed results for the test samples from the Definitive Test are given in Table 2. 

Figure 4 and Figure S showed the chromatographs of the control sample and the treated 

sample from the Definitive Test. The results indicated that concentration of SOS07 was 

stable (within 80% of the initial concentration) in the water during the test period. Thus 

Semi-static method (24h-renewal) used in the Definitive Test was reasonable. 

6.3 Test Condition 

During the Definitive Test, the pH, dissolved oxygen concentration, total hardness and 

temperature of the control and treatment groups were showed in Table 3. 

During the whole test period, the pH values of the control mediums and test mediums 

were between 7.36 and 7.S6, and the Dissolved Oxygen (DO) values varied from 101% 

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to 108% of the air saturation at the test temperature, and the total hardness was in the 

range of 119 mg (CaCO])/L to 125 mg (CaCO])/L During the test, the temperature of 

the test mediums was maintained in the range of23.2°C to 23.5°C. 

6.4 Mortality and Effects 

Table 5 and Table 6 show the mortality data during the range-finding test and Definitive 

Test respectively. Table 7 showed the observations during the Definitive Test. All fish in 

the control were alive and appeared normal. The treated fishes were observed to be lying 

on side or back, or abnormally swift swimming and Tumbling with the test concentration 

no less than 0.4 mglL No fish died in treatments of 0.1 mglL and 0.2 mglL All Fishes 

were dead after 96h-exposure in treatments of 1.6 mglL. 

6.5 Conclusions 

The results showed that under valid Semi-static test conditions (24 h-renewal), the 96 

of the test item to zebra fish is 0.78 mglL (analysed concentration 0.71 mglL), 

with 95% confidence limit of 0.71 mglL to 0.85 mglL The maximum tested 

concentration causing no mortality (NOEC) is 0.2 mglL (analysed concentration 0.191 

mg/L). The lowest tested concentration at which the substance is observed to have a 

lethal effect (LOEC) is 0.4 mglL (analysed concentration 0.389 mglL). The minimum 

concentration causing 100% mortality was 1.6 mglL (analysed concentration 1.37 mglL). 

LC50 0.78 mg/L (analysed concentration 0.71 mglL); 

NOEC 0.2 mglL (analysed concentration 0.191 mglL); 

LOEC 0.4 mg/L (analysed concentration 0.389 mg/L); 

The minimum concentration causing 100% mortality = 1.6 mg/L (analysed concentration 

1.37 mg/L). 

According to "The guidelines for the hazard evaluation of new chemical substances 

(HJ/T 154-2004)", S0507 belongs to the category of very toxic chemical for zebra fish 

(Brachydanio rerio). 

7 HEALTH & SAFETY 

(n order for PEAPC to comply with Law of the People's Republic of China on the 

Prevention and Treatment of Occupational Diseases 200 I, and the current Control of 

Substances Hazardous to Health Regulations, it is a condition of undertaking the study 

that the Sponsor provide PEAPC with all information available to it regarding known or 

potential hazards associated with the handling and use of any substance supplied by the 

Sponsor to PEAPC. The Sponsor also complied with all current legislation and 

Page 190f28 

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Sanitized Version 

Lab of Pesticide Assessment and Pollution No.. R201ONC037·01

regulations concerning shipment of substances by road, rail, sea or air. 

Such information in the form of a completed PEAPC test item data sheet must be 

received at PEAPC before the test item can be handled in the laboratory. 

8 MAINTENANCE OF RECORDS & DOCUMENTATION 

All raw data arising from the performance of this study will remain the property of the 

Sponsor. 

Records and documentation relating to this study (including electronic records) will be 

maintained in the archives of PEAPC for a period of five year from the date on which the 

Study Director signs the final report. This includes the Study Protocol, raw data, a copy 

ofthe final report. 

Types of sample which are unsuitable, by reason of instability, for long term retention 

and archiving will be disposed of after the periods stated in PEAPC Standard Operating 

Procedures. Test item remaining will be retained by PEAPC in its archive for a period of 

one year from the date on which the Study Director signs the final report. After such 

time, the Sponsor will be contacted and his advice sought on the return, disposal or 

further retention of the materials. 

If requested, PEAPC will continue to retain the materials subject to a reasonable fee as 

agreeable to the Sponsor. 

PEAPC will retain the Quality Assurance records relevant to this study and a copy ofthe 

final report in its archive indefinitely. 

9 REFERENCE 

[I] OECD. 203 Fish, Acute Toxicity Test. Paris: 17 July 1992. 

[2] SEPA. The guidelines for the testing of chemicals. HJ/T 153-2004.2004. 

[3] SEP A. The guidelines for the hazard evaluation 	of new chemical substances. HJ/T 

154-2004. 2004. 

[4] SEPA. The guidelines for the testing 	of chemicals. Beijing: China Environmental 

Sciences Publishing House. 2004. 

[5] Part 	 C.I (Acute Toxicity for Fish) of EC Methods for the Determination of 

Ecotoxicity; - Annex to Council Regulation (EC), L 142 (2008). 

[6] Ecological 	Effects Test Guidelines, OPPTS 850.1075, Fish Acute Toxicity Test, 

Freshwater and Marine. EPA 712-C-96-118, April 1996. 

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Lab of Pesticide and Pollution No.: R201ONC037·01 

TABLES 

Table 1 Recovery of S0507 in Test Medium 

Nominal Measured Recovery Mean Recovery RSO

Concentration (mg/L) Concentration (mg/L) Rate (%) rate (%) (%)

102 

Table 2 Stability Test Results ofS0507 in Test Medium (Definitive Test) -------, 

Analysed Concentration (mg/L) Nominal 

24 h 48 h 72 h 

Concentration 

(mglL) Oh (beforel after 

renewal) 

(before/after 

renewal) 

(before/after 

renewal) 

96 h 

0 NO.G.l NO. ND. NO. NO. ! 

0.1 0.103 0.094/0.096 0.089/0.103 0.0910.100 0.089 

I 

0.2 

0.4 

i 0.185 

0.410 

0.186/0.184 

0.380/0.379 

0.184/0.208 

0.378/0.440 

0.185/0.215 

0.378/0.368 

0.184 

0.376 

0.8 ! 0.790 0.753/0.685 0.736/0.859 0.739/0.664 0.730 

1.6 I I 1.61 1.40/1.31 1.384/1.29 1.34311.33 i 1.31 

G.lNO: not detected, since below the sensitivity of the analytical method. 

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Lab of Pesticide Environmental Assessment and Pollution No. R2010NC037-01

Table 3 Water Quality Parameters of Test Solutions during the Definitive Test 

Nominal I I , Dissolved I ...----, , 

Concentration I : Temperature i Hardness 

Duration(h) pH CC) oxygen i (mg/LCaC03)

(mglL) (%) 

0 I 7.46 23.5 102 125 

24( expired/fresh) 7.4417.45 23.5/23.4 1011105 120/121 

I 

0 i 48(eXPired/fre~ 7.5017.48 23.2123.3 1041102 120/120 

I 72( expired/fres 7.5117.46 23.4123.3 101/103 1211122 

i 96 7.48 23.5 101 119 

I 0 7.51 t: 23.5 1031104 120 

24( expired/fresh) 7.4817.50 23.5/23.4 1061103 i 120/121 

0.1 48( expi red/ fresh) 7.5217.52 23.2/23.3 1041102 121/120 

I 

~?2(expired/fresh) 7.5517.52 23.4123.3 101/103 1221121 I 

96 7.53 23.5 01 121 

0 7.53 23.5 1021104 120 ,-­

24( expired/fresh) 7.5617.50 23.5/23.4 1051103 121/120 

0.2 48( expired/fresh) 7.5317.52 23.2/23.3 1041105 1191120 

72( expired/fresh) 7.5517.55 23.4123.3 1011104 1201121 

-" 

96 7.36 23.5 102 121 

0 7.52 23.5 104 120 

24( expired/fresh) 7.5517.50 23.5123.4 10 11102 1211120 

0.4 I 48( expired/fresh) 7.4817.52 23.2/23.3 101/103 1191121 

• 72( expired/fresh) 7.5217.54 23.4/23.3 1021105 120/122

r 96 7.48 23.5 102 121 

0 7.55 23.5 106 119 

24( expired/fresh) , 7.5317.52 23.5/23.4 104/103 1201121 

0.8 48( expired/fresh) 7.46/7.50 23.2/23.3 1011103 121/120 

_ 72( expired/fresh) 7.5017.52 23.4/23.3 101/102 122/120 .... 

96 7.49 23.5 108 121 

0 7.56 23.5 104 119 

24( expired/fresh) 7.5517.54 23.5/23.4 101/102 120/121 

1.6 48( expired/fresh) 7.4517.53 23.2/23.3 1011103 1211122 

I 

72( expired/fresh) 7.5217.55 23.4/23.3 1001102 122/121 

96 7.50 23.5 101 121 

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Lab of Pesticide Environmental and 

Table 4 Toxicity of Potassium Dichromate to Brachydanio rerio 

! ! ! The Number of the Dead 

Concentration Number 

I (mg/L) ofFish I 6h 12 h 18 h 24 h i 

I 

10 0 0 0 0 

0 I 10 0 0 0 0 l 

, 10 0 0 0 0 

I 

10 0 0 0 0 =J 100 10 0 0 0 0 

10 0 0 0 0 

I 

10 0 1 2 2 

200 10 0 2 3 3 

i 10 0 0 1 2 i 

I 

10 0 2 3 4 

300 10 1 3 3 4 

10 I 3 4 5 

I 

10 1 3 6 I 7 

400 10 2 4 6 7 

i 10 2 4 7 8 i 

\0 3 6 9 10 

500 10 3 6 10 10 

\0 2 4 \0 10 

LCso (mglL) >500 480 311 282 

95% confidence limit (mgiL) - 427 ~ 538 292 - 331 265 - 300 i 

Table 5 Mortality during the Range-finding Test 

Nominal Concentration The Number of the Dead Fish Initial Number 

(mg/L) ofFish 24 h 48 h 72h 96 h 

0 5 0 0 0 0 

I 5 0 4 5 5 

10 5 5 5 5 5 

100 5 5 i 5 5 5 

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Lab of Pesticide Environmental No. R2010NC037·01

Table 6 Mortality during the Definitive Test 

Nominal Analysed Initial The Number of the Dead Fish 

Concentration Concentration • Number 

(mglL) (mglL) ofFish 24 h 48 h 72h 96 h 

10 0 0 0 0 

0 ND i 10 0 0 0 0 

10 0 0 0 0 

10 0 0 0 0 

0.1 0.0955 10 0 0 0 0 

10 0 0 0 0 

10 0 I 0 D 0 

0.2 0.191 10 0 A 0 0 

I 10 0 0 0 0 

10 0 0 ) 2 

0.4 0.389 10 0 0 1 2 ! 

10 0 0 0 I 

IO 0 1 2 4 

0.8 

i 

0.745 10 0 0 I 3 I 

10 0 0 I 2 4 

I 10 0 9 IO 10 

1.6 1.37 10 0 9 10 10 

10 0 10 10 10 

LCso (mg/L)(nominal) > 1.6 1.15 0.96 0.78 

95% confidence limit (mglL) (nominal) - 1.12-1.18 0.90-1.02 0.71-0.85 I 

LCso (mglL)(analysed) >1.37 1.02 0.87 0.71 

I 95% confidence limit (mglL) (analysed) - 1.00-1.05 0.82-0.92 0.66-0.771 

The average of the analysed concentrations at O-h, 24-h, 48-h, 72-h, 96-h. 

ND: not detected, since below the sensitivity of the analytical method. 

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Lab "fP"o,i,·iAe Envlfomnental Assessment and Pollution No.: R2010NC037·01 

Table 7 Visual Observations during Definitive Test 

! Nominal Visual Observations I

I Conceot'"tjon 

I 

No. 

i i 

(mg/L) 24 h 48 h 72h 96 h 

I #1 NB NB NB NB 

° #2 NB NB NB NB 

• 

#3 NB NB NB NB 

#1 NB NB NB NB 

I 0,1 ! #2 NB NB NB NB 

#3 NB NB NB NB 

#1 NB NB NB NB 

0.2 I #2 NB NB NB NB 

#3 NB NB NB NB 

#1 NB AS SR&AS SR&AS 

0.4 #2 NB AS SR&AS SR&AS 

#3 NB AS SR&AS SR&AS 

i I #1 TS TS&AS SR&AS i SR&AS 

0.8 #2 TS TS&AS SR&AS SR&AS 

I #3 TS TS&AS SR&AS SR&AS 

#1 TS TS&AS Dead Dead 

I 

1.6 #2 TS TS&AS Dead Dead i 

#3 TS Dead Dead Dead 

Note: Abbreviations of symptoms of intoxication described in the table: 

AK-Strong extended gills OB-Fish mainly at the water surface 

AP-Apathy SA-Mucous secretion 

BA-Distended abdomen SR-Fish lying on side or back 

FV-Fins frayed out at the border SV-Strong ventilation 

GA-Exophthalmus TS-Tumbling during swimming 

KR-Convulsions CC-Color changing 

NB-Normal behaviours AS-Abnormally swift swimming 

Page 25 of28 

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Lab of Pesticide Environmental and Pollution No.. R2010NC037-01 

Table 8 Characteristics of the Dilution Water 

Items Analyzed value Acceptable value 

As 

Cd 

< 0.001 mg IL :::;0.01 mg IL 

:::;0.005 mg IL 

Cr6+ 

Pb 

< 0.000 I mg IL :::;0.05 mg IL 

:::;0.01 mg IL 

Hg 

AI <0.004 mg IL :::;0.001 mg IL 

:::;0.2 mg IL 

Fe 

Mn <0.001 mg IL :::;0.3 mg IL 

:::;0.1 mg IL 

Cu 

Zn 

< 0.0002 mg IL :::;1.0 mg IL 

:::;1.0 mg IL 

Ni 0.01 mg IL :::;0.02 mg IL 

Ag 

pH 

<0.03 mg IL :::;0.1 mg IL 

6.5-8.5 mg IL 

The total hardness 

Visible substance 

< 0.001 mg IL :::;450 mg (CaC03)/L 

None 

methyl parathion 0.002 mg IL :::;0.02 mg IL 

malathion 0.25 mg IL :::;0.25 mg IL 

Parathion 0.001 mg IL :::;0.003 mg IL 

Co 

Total organic carbon (TOC) < 0.001 mg IL 

7.55 mg IL 

122.2 mg IL 

None 

< 0.001 mg IL 

< 0.0005 mg IL 

< 0.001 mg IL 

< 0.001 mg IL 

1.91 mg IL 

-

:::;5 mg IL 

Page 26 of28 

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Lab of Pesticide Environmental Assessment and Pollution No., R20IONC037·0I 

FIGURE 


u.s 	 15 2.5 

Coocentrauon (fIlY1.) 

Figure 1 Calibration Curve for the Test Item 

""'=~-~~---.......,----m(r. 1 .50 

Figure 2 Standard UPLC-MS/MS Chromatogram of the Test Item (1 mglL) 

Figure 3 UPLC-MS/MS Chromatogram of the Recovery Test Sample (0.1 mg/L) 

'0-5~O~~-l,O""'O~~~1.5riO-~-20~O~""-~2.""50-'-'-- mir 

Figure 4 UPLC-MS/MS Chromatogram of the Control Sample (Definitive Test) 

Page 27 of28 

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Lab of Pes lie ide Environmental Assessment and Pollution No.: R2010NC'037-01

1(J[J i 

oh(fresh) 24 h( expired) 

100 1.50 2.00 0.50 

24 h(fresh) 96 h(expired) 

Figure 5 UPLC-MS/MS Chromatograms of the Test Item in Definitive Test (1.6 mglL) 

Page 28 of28 

Attachment 14, Page 118 
ATTACHMENT HEADER SHEET 


Attachment Number 015 

Attachment Name 


Ready Biodegradation Test S0507 


Associated PMN Section Number 


N/A 


Does not contain CBI 

Report Number 


KODA 101220491061974 


Page 119 
Sanitized Version 

Lab of Pesticide Environmental and 

SPONSOR 


Intertek(Tianjin) Testing Service Ltd Shanghai Branch 


Report for Ready Biodegradation Test 

( Closed Bottle Method) 

Test Substance: S0507 


Study No.: S2010NC037-02 


Report No.: R2010NC037-02 


Study Director: Liu Jining 

Date of Report Completion 

Dec. 7,2010 

,:;0 " 

g Key Lab of Pesticide Environmental Assessment and Pollution Control, MEP 

Address: 8 Jiang-wang-miao Street, Nanjing 210042, China 

Tel: (+86)2585287074 Fax: (+86) 25 85474630 

Page 1 of 16 

Attachment 15, Page 120 
Sanitized Version 

Assessment No.: R201ONC037·02 

CONTENTS 

SPONSOR AND TEST FACILITy ............................................................................................................. 3 


STATEMENT OF GLP COMPLIANCE .................................................................................................... 4 


QUALITY ASSURANCE STATEMENT .................................................................................................. 5 


STUDY DETAILS PAGE ............................................................................................................................ 6 


I SUMMARY ............................................................................................................................................... 7 


2 INTRODUCTION ..................................................................................................................................... 8 


3 SAFETY PRECAUTIONS ........................................................................................................................ 9 


4 EQUIPMENT & MATERIALS ................................................................................................................ 9 


4.1 Date of Test .............................................................................................................................. 9 


4.2 Inoculum................................................................................................................................... 9 


4.3 Apparatus ................................................................................................................................. 9 


4.4 Preparation of the Test Solutions ............................................................................................. 9 


4.5 Preparation ofthe Test Medium ............................................................................................. 10 


5 TEST DESIGN ......................................................................................................................................... 10 


5.1 Test Methods .......................................................................................................................... 10 


5.2 Validity of Test....................................................................................................................... 11 


6 DATA PROCESSING ............................................................................................................................. I I 


6.1 Calcu lation of Theoretical Oxygen Demand .......................................................................... 11 


6.2 Calculation of Oxygen Consumption ..................................................................................... 12 


6.3 Calculation of Percentage Biodegradability ........................................................................... 12 


7 RESUL TS ................................................................................................................................................. ] 2 


7.1 Test Conditions ....................................................................................................................... 12 


7.2 Conclusions ............................................................................................................................ 12 


8 HEALTH & SAFETY ............................................................................................................................. 13 


9 MAINTENANCE OF RECORDS & DOCUMENTATION ................................................................. 13 


10 REFERENCES....................................................................................................................................... 13 


Table 1 Concentrations of Dissolved Oxygen (DO) .................................................................... 15 


Table 2 Biodegradation ................................................................................................................ 15 


Figure 1 Biodegradation curve ..................................................................................................... 16 


Page 2 of 16 

Attachment 15, Page 121 

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Lab. ofPesticide Environmental Assessment and Pollution No: R2010NC037-02

SPONSOR AND TEST FACILITY 

Sponsor: Intertek (Tianjin) Testing Service Ltd Shanghai Branch 

Address: 2F,Building No.4 Shanghai Comalong Industrial Park, 889 Yi Shan Road, 

Shanghai, China, 200233 


Contact person: Li Xangjun 


Tel: 02160915013 


Test Substance: S0507 

Test Facility: Key Laboratory of Pesticide Environmental Assessment 

and Pollution Control, MEP (PEAPC) 

Address: 8 Jiangwangmiao Street, Nanjing 210042, China 

Tel.: (+86) 2585287074 

Fax: (+86) 25 85474630 

Legal Representative Person: Gao Zhenning, Director-general, professor 

Laboratory Management: Shan Zhengjun, professor 

Head of Department: Shi LiIi, professor 

Study Director: Liu Jining 

Study Personnel: Wu Guanqun, Tian Jing 

Quality Assurance: Ge Feng, assistant professor 

Page J of 16 

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Lab. of Pesticide Environmental Assessment and Pollution No.: R201ONC037-02 

STA TEMENT OF GLP COMPLIANCE 

Study No.: S2010NC037-02 

Report No.: R2010NC037-02 

According to "OECD Guidelines for testing of chemicals", "The guidelines for the testing of 

chemical (HJ/T 153-2004)"and "The guidelines of chemical testing good laboratory practices 

( (HJ/T 155-2004) " issued by State Environmental Protection Administration (SEPA) of the 

People's Republic of China, this experiment was conducted under CMA (China Metrology 

Accreditation) and CNAS (China National Accreditation Service for Conformity Assessment, 

CNAS) experimental conditions at our laboratory. The experimental protocol was strictly carried 

out in the process of the experiment, and the present report has reflected the experimental results 

truly and correctly. 

(Study Director) Date: 

(Laboratory Management) Date: 

Page 4 of 16 

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Lab. of Pesticide Assessment and Pollution No.: R2010NC037·02 

QUALITY ASSURANCE STATEMENT 


Study No.: S2010NC037-02 


Report No.: R20IONC037-02 


This experiment was carried out strictly in accordance with the experimental protocol. It is 

hereby certified that what the present report describes has accurately reflected the raw data of the 

experiment. 

Type of Phase/Process 

inspections Start inspection date 

Date 

i End inspection date Reporting date 

Protocol 

Study Draft Report i 

Process Measure 

Sept. 6 2010 

Dec. 1,2010 

Oct. 8,2010 

Sept. 62010 Sept. 6 2010 

Dec. 1,2010 Dec. 1,2010 

Oct. 8,2010 I Oct. 8, 2010 i 

(Person responsible for QAU) Date: 

Page 5 ofl6 

Attachment 15, Page 124 
Sanitized Version 

Lab. of Pes No.: R2010NC037-02 tic, de Environmental Assessment and Pollution 

STUDY DETAILS PAGE 

Study number: 

Report number: 

Study title: 

Test substance: 

Identity: 

Substance Name: 

Chemical formula: 


Molecu lar weight: 


Appearance: 


Storage conditions: 


Management: 


Head of Department: 


Study Director: 


Person attending to routine duties 

and technical queries in the 

temporary absence of the Study 

Director: 

Study Director contact details: 

Location of study: 

Study dates: 

Start: 

Completion: 

S201ONC037-02 

R20 I ONC037-02 

Ready biodegradation 

S0507 

S0507 

3H-lndolium, 2-[2-[3-[( I ,3-dihydro-1 ,3,3-trimethyl-2H-indol-2­

ylidene)ethylidene ]-2-[( I-phenyl-I H-tetrazol-5-yl)thio ]-1­

cyclohexen-l-yl]ethenyl]-I ,3 ,3-trimethyl-, chloride 

C39H4IN6S'CI 

661.31 


Solid 


Room temperature 


Shan Zhengjun 

Shi Lili 

Liu Jining 

Shi Lili. Formal GLP responsibilities will be assumed by 

Management. 

Liu Jining 

Telephone: +862585287091 

Facsimile: +862585474630 

Email:lin@nies.org 

Key Lab of Pesticide Environmental Assessment and Pollution 

Control, MEP(PEAPC) 

8 liwang-wang-miao Street, Nanjing 210042 

Jiangsu, China 

Sept. 9, 2010 


Oct. 8, 2010 


Page 6 of 16 

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Lab.ofPesttcide ~o.: R2010NC037-02

1 SUMMARY 

The study was conducted according to the guidelines of SEPA HJ/T 153-2004, "the guidelines 

for the testing of chemicals"; Part CA-E (Closed Bottle Test) of EC Methods for the 

Determination of Ecotoxicity; Annex to Council Regulation (Eq, L 142 (2008); OECD 

Procedure 3010; Closed Bottle test and US Environmental Protection Agency (EPA), Office of 

Prevention, Pesticides and Toxic Substances, (OPPTS) Method 835.3110 (0), adopted January 

1998; GB/T 21831-2008, Chemicals-Ready biodegradability: Closed bottle test. Under valid 

conditions, the ready biodegradability of S0507 was determined in a 28-day dissolve oxygen 

depletion using secondary effluent from a domestic waste water treatment plant. 

During the test, the temperature was kept at 20D C ± 2°e. The test is valid because the level of 

biodegradation of the reference substance reaches 68.1 % within 14 days. Oxygen consumption in 

inoculum blanks is 1.04 mg Oilitre after 28-day test. Thus the study met the acceptability 

criteria prescribed by the protocol and was considered valid. 

Finally the results showed that under valid conditions, 28-day biodegradation of the and S0507 

reach 0%, 14 day of reference substance (sodium benzoate) reach 68.1%, which indicated that 

S0507 has no ready biodegradability. 

Page 7 of 16 

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Lab. of Pesticide Environmental Assessment /',;0 R201ONC037-02

2 INTRODUCTION 

The purpose of this test is to determine the ready biodegradability of water-soluble organic 


chemicals in an aerobic, aqueous medium by monitoring the consumption of oxygen in closed 


bottles, containing the test substance and an inoculum of sewage micro-organisms, over a 28-day 


period. The test may also be used to assess the biodegradability of insoluble and volatile 


substances. It is designed to meet the requirements of SEPA HJ/T 153-2004, "the guidelines for 


the testing of chemicals", Part CA-E (Closed Bottle Test) of EC Methods for the Determination 


of Ecotoxicity; Annex to Council Regulation (EC), L 142 (2008), OECD Procedure 3010, Closed 


Bottle test and US Environmental Protection Agency (EPA), Office of Prevention, Pesticides and 


Toxic Substances, (OPPTS) Method 835.3110 (0), adopted January 1998. 


The method is not applicable to test substances that are inhibitory to aerobic sewage 


micro-organisms at the test concentration. 


Degradation is expressed as the ratio of the biochemical oxygen demand (BOD) and either the 


theoretical oxygen demand (ThaD) or the chemical oxygen demand (COD) of the test substance. 


Information on the relative proportions of the major components of the test substance together 


with their empirical formulae or COOs are therefore necessary prerequisites to this test. 


Substances are considered to be "readily biodegradable" if the BOD is equal to or greater than 


60% of the ThaD (or COD) within ten days of degradation exceeding 10% during the 28-day 


test period. Because of the stringency of this type of test, a BOD equivalent to less than 60% of 


the ThaD or COD within 28-days does not necessarily mean that the substance is not 


biodegradable under environmental conditions. Conditions more favourable to degradation, such 


as a higher concentration of sewage micro-organisms or a longer exposure period, may be 


necessary to establish biodegradation. 


In this test, test solutions will be prepared in an inorganic salts medium, inoculated with a small 


number of micro-organisms from a mixed popUlation, and kept in closed bottles in the dark at a 


temperature of 20 ± 2°C. Controls will comprise vessels containing inoculated mineral salts 


medium together with inoculated mineral salts medium with a measured quantity of a reference 


substance. Further inoculated bottles will contain the reference substance and the test substance 


(inhibition of the degradation of the reference will indicate that the test substance is toxic to the 


bacterial inoculums). The progress of degradation will be followed by the determination of the 


concentration of dissolved oxygen in test and control bottles. Results will be expressed as mg O2 


consumed per mg test substance divided by the ThaD or the COD. 


Where a test substance is known to have a low ThaD or is expected to be poorly degradable, the 


inclusion of at least one higher test concentration is recommended to ensure that measurable 


reductions in oxygen concentration are obtained during the test. 


Page 8ofl6 

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Environmental Assessment and Pollution 

The test is valid if the level of biodegradation of the reference substance achieves 60% or more 

within 14 days after the start of the test and, following biodegradation of the test substance, the 

level of dissolved oxygen does not fall below 0.5 mg 02llitre. Oxygen consumption in inoculated 

blanks must not exceed 1.5 mg 02/L after 28 days of incubation. 

3 SAFETY PRECAUTIONS 

Sewage effluents may contain potentially pathogenic micro-organisms. Appropriate protective 

clothing was therefore worn when collecting and handling sewage. Test substance (S0507) was 

treated as hazardous substances and appropriate precautions were taken to avoid breathing dust 

or vapour, and any contact with skin or clothing. 

4 EQUIPMENT & MATERIALS 

4.1 	Date of Test 

Sept. 9. 20 10 ~ Oct. 8, 2010. 

4.2 Inoculum 

Samples of secondary effluent were obtained as the test inoculum from a sewage plant treating 

predominantly domestic sewage (Nanjing Chengdong Sewage Treatment Plant). Aerobic 

conditions were maintained in the effluent by aeration from a laboratory supply of oil-free 

compressed air. 

On the day of the test, the test inoculum was prepared by filtering the sample of sewage effluent 

through a coarse filter, (discarding the first 200 mL) and collecting the remaining filtrates. This 

inoculum was maintained under aerobic conditions in the test area until use. 

4.3 Apparatus 

Incubator (MIR-253, Sanyo, Japan) 

pH-meter (HQ40d, HACH, American) 

BOD-Bottle (250 mL, blown, Jintan Jingbo Experiment Instrument Factory) 

Dissolved oxygen (HQ40d, HACH, American) 

4.4 Preparation of the Test Solutions 

Stock solution for the reference substance (1000 mglL): Dissolved 0.50 I g of the reference 

substance (sodium benzoate) was prepared in 500 mL ultra-pure water. 

Stock solution for the test substance (1000 mglL): Dissolved 0.50 g of the test substance was 

prepared in 500 mL ultra-pure water. 

Page 9 of 16 

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Lab. of Pesticide Environmental Assessment and Pollution No.: R201ONC037-02 

In line with nonnal practice in this type of study, an assessment of the stability of the test 

substance formulation and achieved concentration were not conducted. 

4.5 Preparation of the Test Medium 

The dilution medium was prepared by adding I mL of each of the following stock solutions, 

prepared in pre-aerated pure water to each I itre of pure water. 

Stock solution A 

KH2P04 (potassium dihydrogen phosphate) 

K2HP04 (dipotassium hydrogen phosphate) 

NazHP04.2H20 (disodium monohydrogen phosphate dihydrate) 

NH4CI (ammonium chloride) 

giL 

8.50 

21.75 

33-40 

0.50 

Dissolved the above reagents in water, and set the volume I liter, the pH of 

this solution should be 7.4 

Stock solution B 

CaCh (calcium chloride) 27.50 

Stock solution C 

MgS04 .7HzO (magnesium sulphate heptahydrate) 22.50 

Stock solution D 

FeCb.6H20 (iron (lll) chloride hexahydrate) 0.25 

5 TEST DESIGN 

5.1 Test Methods 

Four groups of "inoculum control", "test", "reference control" and "toxicity control" were 

assigned in this test, with 2 replicates each. 

Firstly appropriate 0.6 mL stock solution of test substance, then added to "test" and "toxicity 

control" BOD-bottle. Secondly, appropriate 0.6 mL of stock solution of sodium benzoate was 

added to BOD-bottles of "reference control" and "toxicity control"; then 0.3 mL inoculum was 

added to all BOD-bottles. 

a) Inoculum control: containing inoculum only (1 mLlL); 


b) Test: containing test substance (2 mg/L) and inoculum (I mLlL); 


c) Reference control: containing reference substance (2 mg/L) and inoculum (\ mULl; 


d) Toxicity control: containing test substance (2 mg/L), reference substance (2 mg/L) and 


inoculum (\ mULl; 

The BOD bottles would be full filled (Total Volume: 300 mL) with the test medium that had 

been aerated for 20 minutes and allowed to stand at the test temperature for at least 20 h. The 

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concentrations of dissolved oxygen in duplicate vessels of each "test", "inoculum control", 

"reference control" and "toxicity control" group were determined after addition of the prepared 

solutions to the BOD bottles (0 d) and the remaining bottles were incubated at 20°C ± 2°C in 

darkness. Subsequent determinations of the concentration of dissolved oxygen in duplicate 

vessels taken from each group were made on 5 d, 7 d, 11 d, 14 d, 18 d, 21 d, 25 d and 28 d. 

The pH and temperature of the contents of each bottle were measured after the concentration of 

dissolved oxygen had been determined. 

Determinations of the dissolved oxygen (DO) in the "toxicity control" group were made at the 

start and after five days. If the DO in "toxicity control" after 5 days was more than 60% or the 

test substance is toxic to the inoculum, the experiment should be stopped. Otherwise the 

experiment would not be broken off until it ends. 

5.2 Validity of Test 

The test is considered valid if 

a)The level of biodegradation of the reference substance achieves 60% or more within 14 days 

after the test starts, and 

b) Following biodegradation of the test substance, the level of dissolved oxygen does not fall 

below 0.5 mg Oz/L, and 

c)Oxygen consumption in inoculated blanks must not exceed 1.5 mg O/L after 28 days of 

incubation. 

6 DATA PROCESSING 

6.1 Calculation of Theoretical Oxygen Demand 

The theoretical oxygen demand (ThOD) of the test or reference substance with a molecular 

formula of 

is calculated in the following way assuming that carbon is mineralised to COz, hydrogen to H20, 

phosphorus to P205 and sodium to Na20. Halogens are presumed to be eliminated as hydrogen 

halides and nitrogen as ammonia. 

ThODNH3 16 [2c + 1/2 (h-cl-3n) + 3s + 5/2p + lI2na - 01 mg02/mg 

MW 

Nitrogen in a test substance may be eliminated as ammonia, nitrite or nitrate. The higher 

oxidation states give a consequent increase in the ThOD of+ 3/2n (nitrite) and + 5/2n (nitrate). 

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The ThODNH3 of test substance and reference substance are 2.20 mg 02/mg and 1.67 mg 02/mg. 

6.2 Calculation of Oxygen Consumption 

On each measurement occasion the oxygen content (mg02!L) of appropriate control, reference 

and test bottles is determined. The blank-corrected level of oxygen consumption (mg02/L) by 

test or reference mixtures is calculated by subtracting the mean levels of oxygen consumed by 

the inoculum blanks. 

The BOD (mg02/mg) of test and reference substance is calculated by dividing the 

blank-corrected level of oxygen consumption in the test or reference mixtures by the appropriate 

test concentration. 

BOD (mg 02/mg test or reference substance) 

mg (test or reference substance) /L 

Where: 


T(o) mean mg02/L content in the bottles containing test or reference substance on Day 0; 


T(n) = mg02/L content in the test or reference substance bottles on Day n; 


C(o) = mean mg02/L content in the control bottles on Day 0; 


C(n) mean mg02/L content in the control bottles on Day n; 


n = Day number sample measured. 


6.3 Calculation of Percentage Biodegradability 

The percentage degradation after n days of the test were calculated by dividing the amount of 

oxygen consumed per mg oftest substance by the ThOD or COD in the following way: 


mg02 consumed/mg test substance 0
 Degradability (%) ----~--------~----------xl 0 


ThO 0 


7 RESULTS 

7.1 Test Conditions 

During the test, the temperature was kept at 20°C ± 2°C. The test is valid because the level of 

biodegradation of the reference substance achieves 68.1 % within 14 days. Oxygen consumption 

in inoculated blanks must is 1.04 mg 0211itre after 28 days of incubation. Therefore the test 

would be considered valid. 

7.2 Conclusions 

Table 1 and Table 2 showed the biodegradation data during the 28-d test. The result indicated 

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Lab, ofPestie.d. Environmental No,: R20 IONC037·02 

14-day biodegradation of the reference substance (sodium benzoate) reached 68.1%. 28 d 

biodegradation of test substance reached 0%, which indicated that test substance has no ready 

biodegradability. 

8 HEALTH & SAFETY 

In order for PEAPC to comply with Law of the People's Republic of China on the Prevention 

and Treatment of Occupational Diseases 200 I, and the current Control of Substances Hazardous 

to Health Regulations, it is a condition of undertaking the study that the Sponsor provide PEAPC 

with all information available to it regarding known or potential hazards associated with the 

handling and use of any substance supplied by the Sponsor to PEAPC. The Sponsor also 

complied with all current legislation and regulations concerning shipment of substances by road, 

rail, sea or air. 

Such information in the form of a completed PEAPC test substance data sheet must be received 

at PEAPC before the test substance can be handled in the laboratory. 

9 MAINTENANCE OF RECORDS & DOCUMENTATION 

All raw data arising from the performance of this study will remain the property of the Sponsor. 

Records and documentation relating to this study (including electronic records) will be 

maintained in the archives of PEA PC for a period of five year from the date on which the Study 

Director signs the final report. This includes the Study Protocol, raw data, a copy of the final 

report. 

Types of sample which are unsuitable, by reason of instability, for long term retention and 

archiving were disposed of after the periods stated in PEAPC Standard Operating Procedures. 

Test substance remained will be retained by PEA PC in its archive for a period of one year from 

the date on which the Study Director signs the final report. After such time, the Sponsor will be 

contacted and his advice sought on the return, disposal or further retention of the materials. 

If requested, PEAPC will continue to retain the materials subject to a reasonable fee being agreed 

with the Sponsor. 

PEAPC will retain the Quality Assurance records relevant to this study and a copy of the final 

report in its archive indefinitely. 

10 REFERENCES 

[I] 	 SEPA. The guidelines for the testing of chemicals. HJ/T 153-2004. 2004. 

[2] 	 SEPA. The guidelines for the hazard evaluation of new chemical substances. HJ/T 

154-2004.2004. 

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Lab. ofPesticide Environmental Assessment and Pollution No.: R2010NC037-02 

[3] SEPA. The guidelines of chemical testing good laboratory practices. H1IT 155-2004.2004. 

[4] SEPA. The guidelines for the testing of chemicals. Beijing: China Environmental Sciences 

Publishing House. 2004. 

[5] OBIT 21831-2008, Chemicals-Ready biodegradability: Closed bottle test. 

[6] OECD. 3010 Closed Bottle Test. Paris: 1992. 

[7] Part C.4-E (Closed Bottle Test) of EC Methods for the Determination of Ecotoxicity; Annex 

to Council Regulation (EC), Ll42 (2008). 

[8] US Environmental Protection Agency (EPA). Closed Bottle Test. Office of Prevention, 

Pesticides and Toxic Substances, (OPPTS) Method 835.3110 (0), adopted January 1998. 

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Lab of Pesticide Environmental Assessment and Pollution No,: R2010NC037-02 

Table 1 Concentrations of Dissolved Oxygen (DO) 

Samples Od 

Inoculum 8.61 

control 8.58 

8.63 

Test 

8.59 

Reference 8.60 

control 

5d 

8.52 

8.54 

8.59 

8.57 

8.57 

Toxicity 

control 

7d 

8.36 

8.39 

8.47 

8.44 

8.60 

DO after n days (mglL) 

lid 

8.23 

8.24 

8.34 

8.31 

14 d 

8.14 

8.18 

8.22 

8.25 

8.60 

7.69 

18 d 

8.04 

8.05 

8.16 

8.14 

8.57 

7.69 

21d 

7.83 

7.94 7.64 

8.03 7.90 

8.03 7.91 7.86 

8.60 8.57 8.60 

7.69 7.69 7.69 

Table 2 Biodegradation 

Sample Biodegradation after n days (%) 

5d 7d 11 d 14d 18 d 21 d 25 d 28 d 

Test 

0 0 0 0 0 0 0 0 

0 0 0 0 0 0 0 0 

Reference 25.3 51.8 62.0 67.5 71.6 73.4 77.5 79.9 

control 24.4 50.0 62.0 68.7 69.8 74.3 77.5 80.2 

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Lab, of Pesticide Environmental Assessment and Pollution No" R20 IONC037·02 

Figure I Biodegradation curve 

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