# Patent

## Title
Isoprene synthase variants with improved solubility for production of isoprene

## Abstract

The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a divisional of U.S. patent application Ser. No. 13/662,328, now U.S. Pat. No. 8,735,134, filed Oct. 26, 2012, which claims priority to U.S. Provisional Patent Application No. 61/552,453, filed Oct. 27, 2011, the disclosures of which are herein incorporated by reference in their entirety.


INCORPORATION BY REFERENCE
The content of the following submission on ASCII text file is incorporated herein by reference in its entirety: a computer readable form (CRF) of the Sequence Listing (filename: “643842004110_Sequence_Listing.txt”, date recorded: Jul. 8, 2014, size: 143,146 bytes.)
FIELD OF THE INVENTION
The present invention provides methods and compositions comprising isoprene synthase variants. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.
BACKGROUND OF THE INVENTION
Isoprene (2-methyl-1,3-butadiene) is a volatile hydrocarbon that is insoluble in water and soluble in alcohol. Commercially viable quantities of isoprene can be obtained by direct isolation from petroleum C5 cracking fractions or by dehydration of C5 isoalkanes or isoalkenes (Weissermel and Arpe, Industrial Organic Chemistry, 4th ed., Wiley-VCH, pp. 117-122, 2003). The C5 skeleton can also be synthesized from smaller subunits. It would be desirable, however, to have a commercially viable method of producing isoprene that was independent of nonrenewable resources.
Isoprene monomer is employed in the manufacture of polyisoprene and various copolymers (with isobutylene, butadiene, styrene, or other monomers). Building a strain (prokaryotic or eukaryotic) capable of producing commercially viable levels of isoprene requires optimization of part of or the entire DXP or MVA pathway or both MVA and DXP pathways. A key enzyme in the pathway is isoprene synthase (IspS), which converts the precursor DMAPP to isoprene. Isoprene synthases (IspS) that have been identified include those from plants such as poplar, English oak and kudzu vine. Some of the plant IspS enzymes identified have been partially characterized in part by expression in E. coli and some of the kinetic parameters of these enzymes have been determined in vitro with purified protein. However, the solubility of the native IspS enzymes and even some of the recombinant enzymes are insufficient for commercial production of isoprene in a biological host. Thus, one problem to be solved is the provision of isoprene synthase variants (e.g. with substitutions at specific residues) which have improved solubility such that a greater amount of isoprene can be biologically produced. To solve this problem as described herein, an isoprene synthase with improved solubility may be expressed in a host (e.g. a bacterial host).
All patents, patent applications, articles and publications mentioned herein are hereby expressly incorporated herein by reference.
SUMMARY OF THE INVENTION
The invention provides compositions of variant polypeptides having isoprene activity with improved solubility and methods for making and using such variants for the production of isoprene.
Accordingly, in one aspect, the invention provides for isolated polypeptides having isoprene synthase activity, wherein the polypeptide variant comprises a substitution at residue X288 corresponding to SEQ ID NO:1, and wherein the polypeptide has increased protein solubility compared a parent polypeptide which does not comprise the substitution at residue X288. In one embodiment, the substitution is at S288. In another embodiment, the substitution is S288C. In another embodiment, the polypeptide has at least about 5% to at least about 75% increased protein solubility compared to the parent polypeptide. In another embodiment, the polypeptide is derived from a parent polypeptide isolated from a plant. In another embodiment, the parent polypeptide is isolated from a plant species selected from poplar (Populus sp.), kudzu (Pueraria sp.), English oak (Quercus sp.) or willow (Salix sp.). In another embodiment, the parent species is Populus sp. In another embodiment, in the parent is P. alba, P. tremuloides, P. trichocharpa, P. nigra. In another embodiment, the parent species is Pueraria sp. In another embodiment, in the parent species is Pueraria montana. In another embodiment, the parent species is Quercus sp. In another embodiment, the parent species is Quercus rubur. In another embodiment, the parent species is Salix sp. In another embodiment, the parent species is S. alba or S. baylonica. 
In another aspect, the invention provides for recombinant host cells comprising any of the polypeptides described herein. In one embodiment, the host cell is selected from the group consisting of a bacterial, algal, fungal, yeast, cyanobacterial, or Clostridial cell. In another embodiment, the host cell is a bacterial cell. In another embodiment, the bacterial cell is a gram-positive bacterial cell or gram-negative bacterial cell. In another embodiment, the bacterial cell is selected from the group consisting of E. coli, L. acidophilus, P. citrea, B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans, B. lautus, B. thuringiensis, S. albus, S. lividans, S. coelicolor, S. griseus, Pseudomonas sp., P. alcaligenes, Clostridium sp., Corynebacterium sp., and C. glutamicum cells. In another embodiment, the host cell is an algal cell. In another embodiment, the algal cell is selected from the group consisting of green algae, red algae, glaucophytes, chlorarachniophytes, euglenids, chromista, or dinoflagellates. In another embodiment, the host cell is a fungal cell. In another embodiment, the fungal cell is a filamentous fungi. In another embodiment, the host cell is a yeast cell. In another embodiment, the yeast cell is selected from the group consisting of Saccharomyces sp., Schizosaccharomyces sp., Pichia sp., or Candida sp. In another embodiment, the yeast cell is a Saccharomyces cerevisiae cell.
In another aspect, the invention provides for a crystalline form of a polypeptide comprising the amino acid residues of SEQ ID NO:2 (DW614). In another aspect, the invention provides for a polynucleotide that encodes for isolated polypeptides having isoprene synthase activity, wherein the polypeptide variant comprises a substitution at residue X288 corresponding to SEQ ID NO:1, and wherein the polypeptide has increased protein solubility compared a parent polypeptide which does not comprise the substitution at residue X288. In another aspect, the invention provides for a vector comprising a polynucleotide that encodes for isolated polypeptides having isoprene synthase activity, wherein the polypeptide variant comprises a substitution at residue X288 corresponding to SEQ ID NO:1, and wherein the polypeptide has increased protein solubility compared a parent polypeptide which does not comprise the substitution at residue X288. In another aspect, the invention provides for a recombinant host cell comprising the vector described above. In another aspect, the invention provides for methods of producing isoprene comprising: (a) culturing the recombinant cell of above under conditions suitable for the production of isoprene and (b) producing isoprene.
In another aspect, the invention provides for methods of producing isoprene comprising: (a) culturing the recombinant cell comprising polypeptides having isoprene synthase activity, wherein the polypeptide variant comprises a substitution at residue X288 corresponding to SEQ ID NO:1, and wherein the polypeptide has increased protein solubility compared a parent polypeptide which does not comprise the substitution at residue X288 under conditions suitable for the production of isoprene and (b) producing isoprene.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows a monomer view of wild type IspS showing the location of sites located approximately 8 Å from the active site (A). Dimethylallyl s-thiolodiphosphate and Mg2+ (spheres) are placed based on a structural alignment with PDB 3N0G. B) Close-up view of sites in A.
FIG. 2 shows a plasmid maps for pDW218—MEA P. alba IspS S288C.
FIG. 3 shows a map of pCHL243, containing P. alba Isoprene Synthase with the G491S mutation.
FIG. 4 shows a plasmid map for B. pDW161 pTrc MEA P. alba IspS (S288C G491S)-mMVK.
FIG. 5 shows MEA P. alba IspS containing the S288C mutation is soluble.
FIG. 6 shows MEA P. alba IspS S288C G491S is significantly more soluble than the G491S parent enzyme.
FIG. 7 shows Clustal W alignment between P. alba IspS and S. alba IspS.
FIG. 8 shows a plasmid map of pEWL792.
FIG. 9 shows a plasmid map of pEWL795.
FIG. 10 shows Clustal W alignment between P. alba IspS and S. babylonica IspS.
FIG. 11 shows a plasmid map of pEWL834.
FIG. 12 shows a plasmid map of pEWL851.
FIG. 13 shows western blot analysis of IspS in the supernatant and pellet fraction.
FIG. 14 shows protein solubility analysis of IspS by comparison of IspS in the supernatant and pellet fraction.
FIG. 15 shows a plasmid map of pEWL906.
FIG. 16 shows a plasmid map of pEWL907.
FIG. 17 is a coomassie stained NuPage gel that shows improved Salix IspS solubility due to the S288C mutation.
FIG. 18 is a western blot that shows improved Salix IspS solubility due to the S288C mutation.
FIG. 19 shows wild type IspS with anomalous difference map contoured at 5σ showing location of sulfur atoms.
FIG. 20 shows dimer view of IspS variant A3T/S288C. Chain A is light gray, chain B is dark gray.
FIG. 21 shows a structural alignment of wild type IspS (dark gray) and variant A3T/S288C (light gray).
FIG. 22 shows a structural alignment of wild type IspS (light gray) and variant A3T/S288C (dark gray) showing environment around position 288.
FIG. 23 shows plasmid map pDW196.
FIG. 24 shows plasmid maps pDW204.
FIG. 25 shows positions where the sequence of S. alba differs from P. alba are highlighted. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 26 shows positions where the sequence of S. babylonica differs from P. alba are highlighted. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.



DETAILED DESCRIPTION OF THE INVENTION
The invention provides compositions of variant polypeptides having isoprene activity with improved solubility and methods for making and using such variants for the production of isoprene. In one embodiment, the variant polypeptide has a substitution at X288 residue corresponding to reference sequence SEQ ID NO:1. The sequence of MEA P. alba isoprene synthase is as follows:







(SEQ ID NO: 1)


MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNE


 


KAEFLTLLELIDNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLH


 


GTALSFRLLRQHGFEVSQEAFSGFKDQNGNFLENLKEDIKAILSLYEAS


 


FLALEGENILDEAKVFAISHLKELSEEKIGKELAEQVNHALELPLHRRT


 


QRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRETSRWWR


 


RVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFSFVTIID


 


DIYDVYGTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIA


 


YDNLKDKGENILPYLTKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAW


 


KSSSGPLQLVFAYFAVVQNIKKEEIENLQKYHDTISRPSHIFRLCNDLA


 


SASAEIARGETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKEKL


 


GGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPI


 


LPFER.






The practice of the present invention will employ, unless otherwise indicated, conventional techniques of protein chemistry, molecular biology (including recombinant techniques), microbiology, cell biology, biochemistry, nucleic acid chemistry, and enzymology, which are within the skill of the art. Such techniques are explained fully in the literature, such as, Molecular Cloning: A Laboratory Manual, second edition (Sambrook et al., 1989) and Molecular Cloning: A Laboratory Manual, third edition (Sambrook and Russel, 2001), (jointly and individually referred to herein as “Sambrook”). Oligonucleotide Synthesis (M. J. Gait, ed., 1984); Animal Cell Culture (R. I. Freshney, ed., 1987); Handbook of Experimental Immunology (D. M. Weir & C. C. Blackwell, eds.); Gene Transfer Vectors for Mammalian Cells (J. M. Miller & M. P. Calos, eds., 1987); Current Protocols in Molecular Biology (F. M. Ausubel et al., eds., 1987, including supplements through 2001); PCR: The Polymerase Chain Reaction, (Mullis et al., eds., 1994); Current Protocols in Nucleic Acid Chemistry John Wiley & Sons, Inc., New York, 2000); and Agrawal, ed., Protocols for Oligonucleotides and Analogs, Synthesis and Properties Humana Press Inc., New Jersey, 1993). Accordingly, the terms defined immediately below are more fully described by reference to the Specification as a whole.
Furthermore, the headings provided herein are not limitations of the various aspects or embodiments of the invention, which can be had by reference to the specification as a whole. Accordingly, the terms defined immediately below are more fully defined by reference to the specification as a whole. Nonetheless, in order to facilitate understanding of the invention, a number of terms are defined below.
DEFINITIONS
Unless defined otherwise herein, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although any methods and materials similar or equivalent to those described herein find use in the practice of the present invention, the preferred methods and materials are described herein. Accordingly, the terms defined immediately below are more fully described by reference to the Specification as a whole.
“X” refers to any amino acid residue. However, when in the context of an amino acid substitution (e.g. “X003C”), it is to be understood that “X” refers to an amino acid residue other than the amino acid residue resulting from the substitution (e.g., X is an amino acid residue other than C). In some embodiments, the additional zeros in front of the residue position are not included, thus for example “X003” can also be referred to as “X3” to refer to residue position 3.
“Isoprene” refers to 2-methyl-1,3-butadiene (CAS#78-79-5). It can refer to the direct and final volatile C5 hydrocarbon product from the elimination of pyrophosphate from 3,3-dimethylallyl pyrophosphate (DMAPP). It may not involve the linking or polymerization of one or more isopentenyl diphosphate (IPP) molecules to one or more DMAPP molecules. Isoprene is not limited by the method of its manufacture.
As used herein, the terms “isoprene synthase,” “isoprene synthase variant”, and “IspS,” refer to enzymes that catalyze the elimination of pyrophosphate from dimethylallyl diphosphate (DMAPP) to form isoprene. An “isoprene synthase” may be a wild type sequence or an isoprene synthase variant.
An “isoprene synthase variant” indicates a non-wild type polypeptide having isoprene synthase activity. One skilled in the art can measure isoprene synthase activity using known methods. See, for example, by GC-MS (see, e.g., WO 2009/132220, Example 3) or Silver et al., J. Biol. Chem. 270:13010-13016, 1995. Variants may have substitutions, additions, deletions, and/or truncations from a wild type isoprene synthase sequence. Variants may have substitutions, additions, deletions, and/or truncations from a non-wild type isoprene synthase sequence. The variants described herein contain at least one amino acid residue substitution from a parent isoprene synthase polypeptide. In some embodiments, the parent isoprene synthase polypeptide is a wild type sequence. In some embodiments, the parent isoprene synthase polypeptide is a non-wild type sequence. In various embodiments, the variant will have at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 100%, at least about 110%, at least about 120%, at least about 130%, at least about 140%, at least about 150%, at least about 160%, at least about 170%, at least about 180%, at least about 190%, at least about 200% of the activity of a wild type isoprene synthase. In various embodiments, the variant will have at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% sequence identity to a wild type isoprene synthase. In various embodiments, the number of differing amino acid residues between the variant and the wild type may be one or more, e.g. 1, 2, 3, 4, 5, 10, 15, 20, 30, 40, 50, or more amino acid residues. Wild type isoprene synthases can include any isoprene synthases from plants, for example, kudzu isoprene synthases, poplar isoprene synthases, English oak isoprene synthases, and willow isoprene synthases.
As used herein, an amino acid residue of an amino acid sequence of interest that “corresponds to” or is “corresponding to” or in “correspondence with” an amino acid residue of a reference amino acid sequence indicates that the amino acid residue of the sequence of interest is at a location homologous or equivalent to an enumerated residue in the reference amino acid sequence. One skilled in the art can determine whether a particular amino acid residue position in a polypeptide corresponds to that of a homologous reference sequence. For example, the sequence of an isoprene synthase polypeptide may be aligned with that of a reference sequence (e.g. SEQ ID NO: 1 using known techniques (e.g., basic local alignment search tool (BLAST), ClustalW2, Structure based sequences alignment program (STRAP), or the like). In addition, crystal structure coordinates of a reference sequence may be used as an aid in determining a homologous polypeptide residue's three dimensional structure (see, for example, PCT/US2010/032134 (WO 2010/124146)). In another aspect, equivalent residues may be identified by determining homology at the level of tertiary structure. Using such methods, the amino acid residues of an isoprene synthase polypeptide or isoprene synthase variant may be numbered according to the corresponding amino acid residue position numbering of the reference sequence. For example, the amino acid sequence of SEQ ID NO: 1 may be used for determining amino acid residue position numbering of each amino acid residue of an isoprene synthase variant of interest.
The term “identical” in the context of two nucleic acids or polypeptide sequences refers to the residues in the two sequences that are the same when aligned for maximum correspondence, as measured using one of the following sequence comparison or analysis algorithms.
As used herein, “homology” refers to sequence similarity or identity, with identity being preferred. Homology may be determined using standard techniques known in the art (see, e.g., Smith and Waterman, Adv. Appl. Math. 2:482 [1981]; Needleman and Wunsch, J. Mol. Biol. 48:443 [1970\; Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]; software programs such as GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package (Genetics Computer Group, Madison, Wis.); and Devereux et al., Nucl. Acid Res. 12:387-395 [1984]). One example of a useful algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pair-wise alignments. It can also plot a tree showing the clustering relationships used to create the alignment. PILEUP uses a simplification of the progressive alignment method of Feng and Doolittle (see Feng and Doolittle, J. Mol. Evol. 35:351-360 [1987]). The method is similar to that described by Higgins and Sharp (see Higgins and Sharp, CABIOS 5:151-153 [1989]). Useful PILEUP parameters including a default gap weight of 3.00, a default gap length weight of 0.10, and weighted end gaps. Another example of a useful algorithm is the BLAST algorithm, described by Altschul et al., (see Altschul et al., J. Mol. Biol. 215:403-410 [1990]; and Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787 [1993]). A particularly useful BLAST program is the WU-BLAST-2 program (see Altschul et al., Meth. Enzymol. 266:460-480 [1996]). WU-BLAST-2 uses several search parameters, most of which are set to the default values. The adjustable parameters are set with the following values: overlap span=1, overlap fraction=0.125, word threshold (T)=11. The HSP S and HSP S2 parameters are dynamic values and are established by the program itself depending upon the composition of the particular sequence and composition of the particular database against which the sequence of interest is being searched. However, the values may be adjusted to increase sensitivity.
The percent sequence identity between a reference sequence and a test sequence of interest may be readily determined by one skilled in the art. The percent identity shared by polynucleotide or polypeptide sequences is determined by direct comparison of the sequence information between the molecules by aligning the sequences and determining the identity by methods known in the art. An example of an algorithm that is suitable for determining sequence similarity is the BLAST algorithm, (see Altschul, et al., J. Mol. Biol., 215:403-410 [1990]). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence that either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. These initial neighborhood word hits act as starting points to find longer HSPs containing them. The word hits are expanded in both directions along each of the two sequences being compared for as far as the cumulative alignment score can be increased. Extension of the word hits is stopped when: the cumulative alignment score falls off by the quantity X from a maximum achieved value; the cumulative score goes to zero or below; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLAST program uses as defaults a wordlength (W) of 11, the BLOSUM62 scoring matrix (see Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1992]) alignments (B) of 50, expectation (E) of 10, M′5, N′-4, and a comparison of both strands.
The BLAST algorithm then performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul, supra). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a isoprene synthase nucleic acid of this invention if the smallest sum probability in a comparison of the test nucleic acid to a isoprene synthase nucleic acid is less than about 0.1, more preferably less than about 0.01, and most preferably less than about 0.001. Where the test nucleic acid encodes an isoprene synthase polypeptide, it is considered similar to a specified isoprene synthase nucleic acid if the comparison results in a smallest sum probability of less than about 0.5, and more preferably less than about 0.2.
Percent “identical” or “identity” in the context of two or more nucleic acid or polypeptide sequences refers to two or more sequences that are the same or have a specified percentage of nucleic acid residues or amino acid residues, respectively, that are the same, when compared and aligned for maximum similarity, as determined using a sequence comparison algorithm or by visual inspection. “Percent sequence identity” or “% identity” or “% sequence identity or “% amino acid sequence identity” of a subject amino acid sequence to a reference amino acid sequence means that the subject amino acid sequence is identical (i.e., on an amino acid-by-amino acid basis) by a specified percentage to the reference amino acid sequence over a comparison length when the sequences are optimally aligned. Thus, 80% amino acid sequence identity or 80% identity with respect to two amino acid sequences means that 80% of the amino acid residues in two optimally aligned amino acid sequences are identical.
“Percent sequence identity” or “% identity” or “% sequence identity” of a subject nucleic acid sequence to a reference nucleic acid sequence means that the subject nucleic acid sequence is identical (i.e., on a nucleotide-by-nucleotide basis for a polynucleotide sequence) by a specified percentage to the reference sequence over a comparison length when the sequences are optimally aligned. Thus, 80% nucleotide sequence identity or 80% identity with respect to two nucleic acid sequences means that 80% of the nucleotide residues in two optimally aligned nucleic acid sequences are identical.
The “percent sequence identity” or “% sequence identity” or “% identity” of a subject sequence to a reference sequence can be calculated by optimally aligning the two sequences and comparing the two optimally aligned sequences over the comparison length. The number of positions in the optimal alignment at which identical residues occur in both sequences is determined, thereby providing the number of matched positions, and the number of matched positions is then divided by the total number of positions of the comparison length (which, unless otherwise specified, is the length of the reference sequence). The resulting number is multiplied by 100 to yield the percent sequence identity of the subject sequence to the reference sequence.
“Optimal alignment” or “optimally aligned” refers to the alignment of two (or more) sequences giving the highest percent identity score. For example, optimal alignment of two polypeptide sequences can be achieved by manually aligning the sequences such that the maximum number of identical amino acid residues in each sequence are aligned together or by using software programs or procedures described herein or known in the art. Optimal alignment of two nucleic acid sequences can be achieved by manually aligning the sequences such that the maximum number of identical nucleotide residues in each sequence are aligned together or by using software programs or procedures described herein or known in the art.
Two sequences (e.g., polypeptide sequences) may be deemed “optimally aligned” when they are aligned using defined parameters, such as a defined amino acid substitution matrix, gap existence penalty (also termed gap open penalty), and gap extension penalty, so as to achieve the highest similarity score possible for that pair of sequences. The BLOSUM62 scoring matrix (see Henikoff and Henikoff, supra) is often used as a default scoring substitution matrix in polypeptide sequence alignment algorithms (e.g., BLASTP). The gap existence penalty is imposed for the introduction of a single amino acid gap in one of the aligned sequences, and the gap extension penalty is imposed for each residue position in the gap. Exemplary alignment parameters employed are: BLOSUM62 scoring matrix, gap existence penalty=11, and gap extension penalty=1. The alignment score is defined by the amino acid positions of each sequence at which the alignment begins and ends (e.g., the alignment window), and optionally by the insertion of a gap or multiple gaps into one or both sequences, so as to achieve the highest possible similarity score.
Optimal alignment between two or more sequences can be determined manually by visual inspection or by using a computer, such as, but not limited to e.g., the BLASTP program for amino acid sequences and the BLASTN program for nucleic acid sequences (see, e.g., Altschul et al., Nucleic Acids Res. 25(17):3389-3402 (1997); see also the National Center for Biotechnology Information (NCBI) website) or CLUSTALW program.
A polypeptide of interest may be said to be “substantially identical” to a reference polypeptide if the polypeptide of interest comprises an amino acid sequence having at least about 60%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the amino acid sequence of the reference polypeptide. The percent identity between two such polypeptides can be determined manually by inspection of the two optimally aligned polypeptide sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two polypeptides are substantially identical is that the first polypeptide is immunologically cross-reactive with the second polypeptide. Typically, polypeptides that differ by conservative amino acid substitutions are immunologically cross-reactive. Thus, a polypeptide is substantially identical to a second polypeptide, e.g., where the two peptides differ only by a conservative amino acid substitution or one or more conservative amino acid substitutions.
A nucleic acid of interest may be said to be “substantially identical” to a reference nucleic acid if the nucleic acid of interest comprises a nucleotide sequence having at least about 60%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the nucleotide sequence of the reference nucleic acid. The percent identity between two such nucleic acids can be determined manually by inspection of the two optimally aligned nucleic acid sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two nucleic acid sequences are substantially identical is that the two nucleic acid molecules hybridize to each other under stringent conditions (e.g., within a range of medium to high stringency).
A “nucleic acid” refers to two or more deoxyribonucleotides and/or ribonucleotides in either single or double-stranded form. It is to be understood that mutations, including single nucleotide mutations, can occur within a nucleic acid as defined herein.
A “recombinant nucleic acid” refers to a nucleic acid of interest that is free of one or more nucleic acids (e.g., genes) which, in the genome occurring in nature of the organism from which the nucleic acid of interest is derived, flank the nucleic acid of interest. The term therefore includes, for example, a recombinant DNA which is incorporated into a vector, into an autonomously replicating plasmid or virus, or into the genomic DNA of an anaerobic microorganism, or which exists as a separate molecule (e.g., a cDNA, a genomic DNA fragment, or a cDNA fragment produced by PCR or restriction endonuclease digestion) independent of other sequences. A recombinant nucleic acid may be obtained using molecular biology techniques that are known in the art, or part or all of a recombinant nucleic acid may be chemically synthesized.
A “heterologous nucleic acid” can be a nucleic acid whose nucleic acid sequence is from another species than the host cell or another strain of the same species of the host cell. In some embodiments, the sequence is not identical to that of another nucleic acid naturally found in the same host cell. In some embodiments, a heterologous nucleic acid is not identical to a wild-type nucleic acid that is found in the same host cell in nature.
An “endogenous nucleic acid” is a nucleic acid whose nucleic acid sequence is naturally found in the host cell. In some embodiments, an endogenous nucleic acid is identical to a wild-type nucleic acid that is found in the host cell in nature. In some embodiments, one or more copies of endogenous nucleic acids are introduced into a host cell.
A nucleic acid or protein of the invention may be in isolated or purified form. As used herein, “isolated,” with respect to nucleic acid or protein, means separated from other components, such as, but not limited to a cell or cell culture. It is preferably in a homogeneous state although it can be in either a dry or aqueous solution. Purity and homogeneity are typically determined using analytical chemistry techniques, such as polyacrylamide gel electrophoresis or high performance liquid chromatography. A protein or nucleic acid that is the predominant species present in a preparation is substantially purified. The term “purified” denotes that a nucleic acid or protein gives rise to essentially one band in an electrophoretic gel. Particularly, “purified” means that when isolated, the isolate contains at least 90%, at least 95%, at least 98%, or more preferably at least 99% of nucleic acid or protein by weight of the isolate.
Purified polypeptides may be obtained by a number of methods including, for example, laboratory synthesis, chromatography, preparative electrophoresis, gel electrophoresis, centrifugation, precipitation, affinity purification, etc. (see, generally, R Scopes, Protein Purification, Springer-Verlag, N.Y. (1982), Deutscher, Methods in Enzymology Vol. 182: Guide to Protein Purification, Academic Press, Inc. N.Y. (1990)).
“Polypeptides” includes polypeptides, proteins, peptides, fragments of polypeptides, and fusion polypeptides. It is also understood that a polypeptide may be coded for by more than one nucleotide sequence due to the degeneracy of the genetic code.
A “heterologous polypeptide” is a polypeptide encoded by a heterologous nucleic acid. In some embodiments, the sequence is not identical to that of another polypeptide encoded by a nucleic acid naturally found in the same host cell. Examples of heterologous proteins include enzymes such as isoprene synthases. In some embodiments, the genes encoding the proteins are naturally occurring genes, while in other embodiments mutated and/or synthetic genes are used.
An “endogenous polypeptide” is a polypeptide whose amino acid sequence is naturally found in the host cell. In some embodiments, an endogenous polypeptide is identical to a wild-type polypeptide that is found in the host cell in nature.
As used herein, the singular terms “a,” “an,” and “the” include the plural reference unless the context clearly indicates otherwise.
Unless otherwise indicated, nucleic acids are written left to right in 5′ to 3′ orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively.
It is intended that every maximum numerical limitation given throughout this specification includes every lower numerical limitation, as if such lower numerical limitations were expressly written herein. Every minimum numerical limitation given throughout this specification will include every higher numerical limitation, as if such higher numerical limitations were expressly written herein. Every numerical range given throughout this specification will include every narrower numerical range that falls within such broader numerical range, as if such narrower numerical ranges were all expressly written herein.
Reference to “about” a value or parameter herein also includes (and describes) embodiments that are directed to that value or parameter per se.
It is understood that all aspects and embodiments of the invention described herein include “comprising,” “consisting,” and “consisting essentially of” aspects and embodiments. It is to be understood that methods or compositions “consisting essentially of” the recited elements include only the specified steps or materials and those that do not materially affect the basic and novel characteristics of those methods and compositions. It is to be understood that this invention is not limited to the particular methodology, protocols, and reagents described, as these may vary, depending upon the context they are used by those of skill in the art.
Isoprene Synthase Variants with Improved Solubility
The invention features compositions of variant polypeptides having isoprene synthase activity and having improved solubility as well as methods for making such polypeptides and methods for producing increased amounts of isoprene. In some embodiments, the polypeptide has at least about 5% to at least about 75% increased protein solubility compared to a parent polypeptide. In some embodiments, the polypeptide has at least about 5%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, or at least about 75% increased protein solubility compared to a parent polypeptide. In particular, these compositions and methods may increase the rate of isoprene production and the total amount of isoprene that is produced. The biosynthetic processes for isoprene production described herein are a desirable alternative to using natural rubber. As discussed further below, the amount of isoprene produced by cells can be greatly increased by introducing a heterologous nucleic acid encoding an isoprene synthase (IspS) variant into the cells.
Additionally, isoprene production by cells containing a heterologous isoprene synthase nucleic acid can be enhanced by increasing the amount of one or more DXP pathway polypeptides (e.g., a 1-deoxy-D-xylulose-5-phosphate synthase (DXS) polypeptide) and/or an isopentenyl diphosphate isomerase (IDI) polypeptide, expressed by the cells. For example, a DXS nucleic acid and/or an IDI nucleic acid can be introduced into the cells. The DXS nucleic acid may be a heterologous nucleic acid or a duplicate copy of an endogenous nucleic acid. Similarly, the IDI nucleic acid may be a heterologous nucleic acid or a duplicate copy of an endogenous nucleic acid. In some embodiments, the amount of DXS and/or IDI polypeptide is increased by replacing the endogenous DXS and/or IDI promoters or regulatory regions with other promoters and/or regulatory regions that result in greater transcription of the DXS and/or IDI nucleic acids. In some embodiments, the cells contain both a heterologous nucleic acid encoding an isoprene synthase polypeptide (e.g., a plant isoprene synthase nucleic acid) and a duplicate copy of an endogenous nucleic acid encoding an isoprene synthase polypeptide.
The encoded DXS and IDI polypeptides are part of the DXP pathway for the biosynthesis of isoprene. DXS polypeptides convert pyruvate and D-glyceraldehyde-3-phosphate into 1-deoxy-D-xylulose-5-phosphate. While not intending to be bound by any particular theory, it is believed that increasing the amount of DXS polypeptide increases the flow of carbon through the DXP pathway, leading to greater isoprene production. IDI polypeptides catalyze the interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). While not intending to be bound by any particular theory, it is believed that increasing the amount of IDI polypeptide in cells increases the amount of IPP that is converted into DMAPP, which in turn is converted into isoprene.
As is further detailed below, in some embodiments, the production of isoprene by cells containing a heterologous isoprene synthase nucleic acid can be augmented by increasing expression of one or more MVA polypeptide(s) in the cells. Exemplary MVA pathways polypeptides include any of the following polypeptides: acetyl-CoA acetyltransferase (AA-CoA thiolase) polypeptides, 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) polypeptides, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) polypeptides, mevalonate kinase (MVK) polypeptides, phosphomevalonate kinase (PMK) polypeptides, diphosphomevalonte decarboxylase (MVD) polypeptides, IDI polypeptides, and polypeptides (e.g., fusion polypeptides) having an activity of two or more MVA pathway polypeptides. For example, one or more MVA pathway nucleic acids can be introduced into the cells. In some embodiments, the cells contain the upper MVA pathway, which includes AA-CoA thiolase, HMG-CoA synthase, and HMG-CoA reductase nucleic acids. In some embodiments, the cells contain the lower MVA pathway, which includes MVK, PMK, MVD, and IDI nucleic acids. In some embodiments, the cells contain the entire MVA pathway, which includes AA-CoA thiolase, HMG-CoA synthase, HMG-CoA reductase, MVK, PMK, MVD, and IDI nucleic acids. The MVA pathway nucleic acids may be heterologous nucleic acids or duplicate copies of endogenous nucleic acids. In some embodiments, the amount of one or more MVA pathway polypeptides is increased by replacing the endogenous promoters or regulatory regions for the MVA pathway nucleic acids with other promoters and/or regulatory regions that result in greater transcription of the MVA pathway nucleic acids. In some embodiments, the cells contain both a heterologous nucleic acid encoding an isoprene synthase polypeptide (e.g., a plant isoprene synthase nucleic acid) and a duplicate copy of an endogenous nucleic acid encoding an isoprene synthase polypeptide.
In some embodiments, at least a portion of the cells maintain the heterologous isoprene synthase, DXS, IDI, other DXP pathway and/or MVA pathway nucleic acids for at least about 5, 10, 20, 50, 75, 100, 200, 300, or more cell divisions in a continuous culture (such as a continuous culture without dilution). In some embodiments of any of the aspects of the invention, the nucleic acid comprising the heterologous or duplicate copy of an endogenous isoprene synthase, DXS, IDI, other DXP pathway and/or MVA pathway nucleic acids also comprises a selective marker, such as a kanamycin, ampicillin, carbenicillin, gentamicin, hygromycin, phleomycin, bleomycin, neomycin, or chloramphenicol antibiotic resistance nucleic acid
Isoprene synthase polypeptides convert dimethylallyl diphosphate (DMAPP) into isoprene. Exemplary isoprene synthase polypeptides include polypeptides, fragments of polypeptides, peptides, and fusions polypeptides that have at least one activity of an isoprene synthase polypeptide. Standard methods can be used to determine whether a polypeptide has isoprene synthase polypeptide activity by measuring the ability of the polypeptide to convert DMAPP into isoprene in vitro, in a cell extract, or in vivo. Isoprene synthase polypeptide activity in the cell extract can be measured, for example, as described in Silver et al., J. Biol. Chem. 270:13010-13016, 1995 and references therein.
In one embodiment, DMAPP (Sigma) is evaporated to dryness under a stream of nitrogen and rehydrated to a concentration of 100 mM in 100 mM potassium phosphate buffer pH 8.2 and stored at −20° C. To perform the assay, a solution of 5 μl of 1M MgCl2, 1 mM (250 μg/ml) DMAPP, 65 μl of Plant Extract Buffer (PEB) (50 mM Tris-HCl, pH 8.0, 20 mM MgCl2, 5% glycerol, and 2 mM DTT) is added to 25 μl of cell extract in a 20 ml Headspace vial with a metal screw cap and teflon coated silicon septum (Agilent Technologies) and cultured at 37° C. for 15 minutes with shaking. The reaction is quenched by adding 200 μl of 250 mM EDTA or by heat inactivation, and isoprene is quantified by GC/MS.
Isoprene Synthase Parent Sequences
Isoprene synthase variants may be generated from a parent isoprene synthase, wherein the parent isoprene synthase may be an isoprene synthase as described herein, including wild type and non-wild type isoprene synthases. Exemplary parent isoprene synthase nucleic acids include nucleic acids that encode a polypeptide, fragment of a polypeptide, peptide, or fusion polypeptide that has at least one activity of an isoprene synthase polypeptide. Exemplary parent isoprene synthase polypeptides and nucleic acids include naturally-occurring polypeptides and nucleic acids from any of the source organisms described herein as well as variant polypeptides and nucleic acids derived from any of the source organisms described herein.
In some embodiments, the parent isoprene synthase is from the family Fabaceae, the family Salicaceae, or the family Fagaceae. In some embodiments, the parent isoprene synthase polypeptide or nucleic acid is a naturally-occurring polypeptide or nucleic acid from Pueraria montana (kudzu) (Sharkey et al., Plant Physiology 137: 700-712, 2005), poplar (such as Populus alba×tremula CAC35696, Miller et al., Planta 213: 483-487, 2001) or Populus alba, aspen (such as Populus tremuloides) Silver et al., JBC 270(22): 13010-1316, 1995), or English Oak (Quercus robur) (Zimmer et al., WO 98/02550). Suitable parent isoprene synthases include, but are not limited to, those identified by GenBank Accession Nos. AY341431, AY316691, AB198180, AJ294819.1, EU693027.1, EF638224.1, AM410988.1, EF147555.1, AY279379, AJ457070, and AY182241. Additional parent sequences are described in PCT/US2009/041581 (WO 2009/132220) and PCT/US2010/032134 (WO 2010/124146).
In various embodiments, the parent isoprene synthase has at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 92%, at least about 94%, at least about 96%, at least about 98%, at least about 99% sequence identity with MEA P. alba. In other embodiments, the parent isoprene synthase has at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 92%, at least about 94%, at least about 96%, at least about 98%, at least about 99% sequence identity with full-length P. alba or complete P. alba. 
Several methods are known in the art that are suitable for generating variants of the enzymes of the present invention, including but not limited to site-saturation mutagenesis, scanning mutagenesis, insertional mutagenesis, random mutagenesis, site-directed mutagenesis, and directed-evolution, as well as various other recombinatorial approaches.
Variants with improved solubility can be made by including a mutation at X288 corresponding to SEQ ID NO:1. In one aspect, X288 is S288. In another aspect, the mutation is S288C. Exemplary methods of measuring solubility is described below in the Examples.
Exemplary Nucleic Acids
Nucleic acids encoding the isoprene synthase variants of the invention are provided and contemplated within the scope of the invention. In various embodiments, the nucleic acid is a recombinant nucleic acid. For instance, in some embodiments, an isoprene synthase variant nucleic acid is operably linked to another nucleic acid encoding all or a portion of another polypeptide such that the recombinant nucleic acid encodes a fusion polypeptide that includes an isoprene synthase variant and all or part of another polypeptide (e.g., a peptide that facilitates purification or detection of the fusion polypeptide, such as a His-tag). In some embodiments, part or all of a recombinant nucleic acid is chemically synthesized. In some aspects, the nucleic acid is a heterologous nucleic acid. By “heterologous nucleic acid” is meant a nucleic acid whose nucleic acid sequence is not identical to that of another nucleic acid naturally found in the same host cell.
In some embodiments, the nucleic acid includes at least or about 50, 100, 150, 200, 300, 400, 500, 600, 700, 800, or more contiguous nucleotides from a naturally-occurring isoprene synthase nucleic acid. In some aspects, the nucleic acid has one or more mutations compared to the sequence of a wild-type (i.e., a sequence occurring in nature) isoprene synthase nucleic acid. In some embodiments, the nucleic acid has one or more mutations (e.g., a silent mutation) that increase the transcription or translation of isoprene synthase nucleic acid. In some embodiments, the nucleic acid is a degenerate variant of any nucleic acid encoding an isoprene synthase polypeptide.
An isoprene synthase nucleic acid can be incorporated into a vector, such as an expression vector, using standard techniques known to one of skill in the art. Methods used to ligate the DNA construct comprising a nucleic acid of interest such as isoprene synthase, a promoter, a terminator, and other sequences and to insert them into a suitable vector are well known in the art. Additionally, vectors can be constructed using known recombination techniques (e.g., Invitrogen Life Technologies, Gateway Technology).
In some embodiments, it may be desirable to over-express isoprene synthase nucleic acids at levels far higher than currently found in naturally-occurring cells. This result may be accomplished by the selective cloning of the nucleic acids encoding those polypeptides into multicopy plasmids or placing those nucleic acids under a strong inducible or constitutive promoter. Methods for over-expressing desired polypeptides are common and well known in the art of molecular biology and examples may be found in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor, 2001.
Exemplary Pathway Polypeptides
As noted above, one or more polypeptides from the DXP pathway and/or MVA pathway can be used to increase the production of isoprene in conjunction with using the isoprene synthase variants described herein. Accordingly, in certain aspects, the one or more nucleic acids encoding one or more MVA pathway polypeptides is a heterologous nucleic acid. In other aspects, the one or more nucleic acids encoding one or more MVA pathway polypeptides is a copy of an endogenous nucleic acid. In any of the aspects herein, one or more MVA pathway polypeptides can be selected from (a) an enzyme that condenses two molecules of acetyl-CoA to form acetoacetyl-CoA; (b) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA (e.g., HMG synthase); (c) an enzyme that converts HMG-CoA to mevalonate; (d) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate; (e) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; (f) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate; and (g) an enzyme that converts isopentenyl pyrophosphate to dimethylallyl diphosphate. In any of the aspects herein, one or more MVA pathway polypeptides is selected from (a) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA (e.g., HMG synthase); (b) an enzyme that converts HMG-CoA to mevalonate; (c) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate; (d) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; and (e) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate.
In any of the aspects herein, the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate can be selected from the group consisting of M. mazei mevalonate kinase, Lactobacillus mevalonate kinase polypeptide, Lactobacillus sakei mevalonate kinase polypeptide, yeast mevalonate kinase polypeptide, Saccharomyces cerevisiae mevalonate kinase polypeptide, Streptococcus mevalonate kinase polypeptide, Streptococcus pneumoniae mevalonate kinase polypeptide, and Streptomyces mevalonate kinase polypeptide, M. Burtonii mevalonate kinase, or Streptomyces CL190 mevalonate kinase polypeptide. In any of the aspects herein, the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate is M. mazei mevalonate kinase.
Upper MVA Pathway Polypeptides
The upper portion of the MVA pathway uses acetyl Co-A produced during cellular metabolism as the initial substrate for conversion to mevalonate via the actions of polypeptides having either: (a) (i) thiolase activity or (ii) acetoacetyl-CoA synthase activity, (b) HMG-CoA reductase, and (c) HMG-CoA synthase enzymatic activity. First, acetyl Co-A is converted to acetoacetyl CoA via the action of a thiolase or an acetoacetyl-CoA synthase (which utilizes acetyl-CoA and malonyl-CoA). Next, acetoacetyl-CoA is converted to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) by the enzymatic action of HMG-CoA synthase. This Co-A derivative is reduced to mevalonate by HMG-CoA reductase, which is the rate-limiting step of the mevalonate pathway of isoprenoid production.
Non-limiting examples of upper MVA pathway polypeptides include acetyl-CoA acetyltransferase (AA-CoA thiolase) polypeptides, acetoacetyl-CoA synthase polypeptides, 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) polypeptides, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) polypeptides. Upper MVA pathway polypeptides can include polypeptides, fragments of polypeptides, peptides, and fusions polypeptides that have at least one activity of an upper MVA pathway polypeptide. Exemplary upper MVA pathway nucleic acids include nucleic acids that encode a polypeptide, fragment of a polypeptide, peptide, or fusion polypeptide that has at least one activity of an upper MVA pathway polypeptide. Exemplary MVA pathway polypeptides and nucleic acids include naturally-occurring polypeptides and nucleic acids from any of the source organisms described herein. Thus, it is contemplated herein that any gene encoding an upper MVA pathway polypeptide can be used in the present invention.
In certain embodiments, various options of mvaE and mvaS genes from L. grayi, E. faecium, E. gallinarum, E. casseliflavus and/or E. faecalis alone or in combination with one or more other mvaE and mvaS genes encoding proteins from the upper MVA pathway are contemplated within the scope of the invention. In other embodiments, an acetoacetyl-CoA synthase gene is contemplated within the scope of the present invention in combination with one or more other genes encoding: (i) 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) polypeptides and 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) polypeptides. Thus, in certain aspects, any of the combinations of genes contemplated in can be expressed in recombinant cells in any of the ways described herein.
Additional non-limiting examples of upper MVA pathway polypeptides which can be used herein are described in International Patent Application Publication No. WO2009/076676; WO2010/003007 and WO2010/148150.
Genes Encoding mvaE and mvaS Polypeptides
In certain embodiments, various options of mvaE and mvaS genes from L. grayi, E. faecium, E. gallinarum, E. casseliflavus and/or E. faecalis alone or in combination with one or more other mvaE and mvaS genes encoding proteins from the upper MVA pathway are contemplated within the scope of the invention. In L. grayi, E. faecium, E. gallinarum, E. casseliflavus, and E. faecalis, the mvaE gene encodes a polypeptide that possesses both thiolase and HMG-CoA reductase activities. In fact, the mvaE gene product represented the first bifunctional enzyme of IPP biosynthesis found in eubacteria and the first example of HMG-CoA reductase fused to another protein in nature (Hedl, et al., J Bacteriol. 2002 April; 184(8): 2116-2122). The mvaS gene, on the other hand, encodes a polypeptide having an HMG-CoA synthase activity.
Accordingly, recombinant cells (e.g., E. coli) can be engineered to express one or more mvaE and mvaS genes from L. grayi, E. faecium, E. gallinarum, E. casseliflavus and/or E. faecalis, to produce mevalonate. The one or more mvaE and mvaS genes can be expressed on a multicopy plasmid. The plasmid can be a high copy plasmid, a low copy plasmid, or a medium copy plasmid. Alternatively, the one or more mvaE and mvaS genes can be integrated into the host cell's chromosome. For both heterologous expression of the one or more mvaE and mvaS genes on a plasmid or as an integrated part of the host cell's chromosome, expression of the genes can be driven by either an inducible promoter or a constitutively expressing promoter. The promoter can be a strong driver of expression, it can be a weak driver of expression, or it can be a medium driver of expression of the one or more mvaE and mvaS genes.
In any of the aspects herein, the recombinant host cells can further comprise one or more nucleic acids encoding one or more 1-deoxy-D-xylulose 5-phosphate (DXP) pathway polypeptides. In one aspect, one or more nucleic acids that encode for one or more DXP pathway polypeptides is a heterologous nucleic acid. In another aspect, the one or more nucleic acids encoding one or more DXP pathway polypeptides is a copy of an endogenous nucleic acid. In another aspect, the one or more DXP pathway polypeptides is selected from (a) 1-deoxy-D-xylulose-5-phosphate synthase (DXS), (b) 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), (c) 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (MCT), (d) 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK), (e) 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS), (f) 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (HDS), and (g) 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (HDR). In another aspect, the DXP pathway polypeptide is DXS.
In another aspect, one of skill in the art can use an alternate metabolic process which can potentially produce three molecules of acetyl-CoA from one molecule of glucose using a pathway which does not rely on the Wood-Ljungdahl pathway enzymes. Instead, it makes use of a phosphoketolase enzyme found in certain organisms, particularly among Bifidobacteria [see, for example, Biology of the Prokaryotes (ed. Lengeler, Drews and Schlegel); Blackwell Science, New York, 1999, p. 299-301; Meile et al., J. of Bacteriology, 2001, 183:9, 2929-36; Jeong et al., J. Microbiol. Biotechnol., 2007, 17:5, 822-829]. Phosphoketolase enzymes allow for formation of acetyl-CoA (via acetyl-phosphate) from xylulose 5-phosphate or fructose 6-phosphate rather than through oxidation of pyruvate as in typical metabolism. Increased biosynthesis of acetyl CoA by the use of a phosphoketolase polypeptide can result in increased productivity of the upper mevalonate-dependent biosynthetic pathway which can substantially increase biosynthesis of mevalonate and, consequently, of downstream isoprenoid precursor molecules such as DMAPP and IPP. Standard methods can be used to determine whether a polypeptide has phosphoketolase peptide activity by measuring the ability of the peptide to convert D-fructose 6-phosphate or D-xylulose 5-phosphate into acetyl-P. Acetyl-P can then be converted into ferryl acetyl hydroxamate, which can be detected spectrophotometrically (Meile et al., J. Bact. 183:2929-2936, 2001). Any polypeptide identified as having phosphoketolase peptide activity is suitable for use in the present invention. Exemplary phosphoketolase nucleic acids include, but are not limited to, a phosphoketolase isolated from Lactobacillus reuteri, Bifidobacterium longum, Ferrimonas balearica, Pedobactor saltans, Streptomyces griseus, and/or Nocardiopsis dassonvillei. 
Lower MVA Pathway Polypeptides
In some aspects of the invention, the cells described in any of the compositions or methods described herein further comprise one or more nucleic acids encoding a lower mevalonate (MVA) pathway polypeptide(s). In some aspects, the lower MVA pathway polypeptide is an endogenous polypeptide. In some aspects, the endogenous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to a constitutive promoter. In some aspects, the endogenous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to an inducible promoter. In some aspects, the endogenous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to a strong promoter. In a particular aspect, the cells are engineered to over-express the endogenous lower MVA pathway polypeptide relative to wild-type cells. In some aspects, the endogenous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to a weak promoter.
The lower mevalonate biosynthetic pathway comprises mevalonate kinase (MVK), phosphomevalonate kinase (PMK), and diphosphomevalonte decarboxylase (MVD). In some aspects, the lower MVA pathway can further comprise isopentenyl diphosphate isomerase (IDI). Cells provided herein can comprise at least one nucleic acid encoding isoprene synthase, one or more upper MVA pathway polypeptides, and/or one or more lower MVA pathway polypeptides. Polypeptides of the lower MVA pathway can be any enzyme (a) that phosphorylates mevalonate to mevalonate 5-phosphate; (b) that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; and (c) that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. More particularly, the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate can be from the group consisting of M. mazei mevalonate kinase, Lactobacillus mevalonate kinase polypeptide, Lactobacillus sakei mevalonate kinase polypeptide, yeast mevalonate kinase polypeptide, Saccharomyces cerevisiae mevalonate kinase polypeptide, Streptococcus mevalonate kinase polypeptide, Streptococcus pneumoniae mevalonate kinase polypeptide, Streptomyces mevalonate kinase polypeptide, Streptomyces CL190 mevalonate kinase polypeptide, and M. Burtonii mevalonate kinase polypeptide. In another aspect, the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate is M. mazei mevalonate kinase.
In some aspects, the lower MVA pathway polypeptide is a heterologous polypeptide. In some aspects, the cells comprise more than one copy of a heterologous nucleic acid encoding a lower MVA pathway polypeptide. In some aspects, the heterologous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to a constitutive promoter. In some aspects, the heterologous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to an inducible promoter. In some aspects, the heterologous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to a strong promoter. In some aspects, the heterologous nucleic acid encoding a lower MVA pathway polypeptide is operably linked to a weak promoter. In some aspects, the heterologous lower MVA pathway polypeptide is a polypeptide from Saccharomyces cerevisiae, Enterococcus faecalis, or Methanosarcina mazei. 
The nucleic acids encoding a lower MVA pathway polypeptide(s) can be integrated into a genome of the cells or can be stably expressed in the cells. The nucleic acids encoding a lower MVA pathway polypeptide(s) can additionally be on a vector.
Exemplary lower MVA pathway polypeptides are also provided below: (i) mevalonate kinase (MVK); (ii) phosphomevalonate kinase (PMK); (iii) diphosphomevalonate decarboxylase (MVD); and (iv) isopentenyl diphosphate isomerase (IDI). In particular, the lower MVK polypeptide can be from the genus Methanosarcina and, more specifically, the lower MVK polypeptide can be from Methanosarcina mazei. In some embodiments, the lower MVK polypeptide can be from M. burtonii. Additional examples of lower MVA pathway polypeptides can be found in U.S. Patent Application Publication 2010/0086978 the contents of which are expressly incorporated herein by reference in their entirety with respect to lower MVK pathway polypeptides and lower MVK pathway polypeptide variant.
Lower MVA pathway polypeptides include polypeptides, fragments of polypeptides, peptides, and fusions polypeptides that have at least one activity of a lower MVA pathway polypeptide. Exemplary lower MVA pathway nucleic acids include nucleic acids that encode a polypeptide, fragment of a polypeptide, peptide, or fusion polypeptide that has at least one activity of a lower MVA pathway polypeptide. Exemplary lower MVA pathway polypeptides and nucleic acids include naturally-occurring polypeptides and nucleic acids from any of the source organisms described herein. In addition, variants of lower MVA pathway polypeptides that confer the result of better isoprene production can also be used as well.
In some aspects, the lower MVA pathway polypeptide is a polypeptide from Saccharomyces cerevisiae, Enterococcus faecalis, or Methanosarcina mazei. In some aspects, the MVK polypeptide is selected from the group consisting of Lactobacillus mevalonate kinase polypeptide, Lactobacillus sakei mevalonate kinase polypeptide, yeast mevalonate kinase polypeptide, Saccharomyces cerevisiae mevalonate kinase polypeptide, Streptococcus mevalonate kinase polypeptide, Streptococcus pneumoniae mevalonate kinase polypeptide, Streptomyces mevalonate kinase polypeptide, Streptomyces CL190 mevalonate kinase polypeptide, Methanosarcina mazei mevalonate kinase polypeptide, and M. Burtonii mevalonate kinase polypeptide. Any one of the promoters described herein (e.g., promoters described herein and identified in the Examples of the present disclosure including inducible promoters and constitutive promoters) can be used to drive expression of any of the MVA polypeptides described herein.
Any one of the cells described herein can comprise IDI nucleic acid(s) (e.g., endogenous or heterologous nucleic acid(s) encoding IDI). Isopentenyl diphosphate isomerase polypeptides (isopentenyl-diphosphate delta-isomerase or IDI) catalyzes the interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (e.g., converting IPP into DMAPP and/or converting DMAPP into IPP). Exemplary IDI polypeptides include polypeptides, fragments of polypeptides, peptides, and fusions polypeptides that have at least one activity of an IDI polypeptide. Standard methods (such as those described herein) can be used to determine whether a polypeptide has IDI polypeptide activity by measuring the ability of the polypeptide to interconvert IPP and DMAPP in vitro, in a cell extract, or in vivo. Exemplary IDI nucleic acids include nucleic acids that encode a polypeptide, fragment of a polypeptide, peptide, or fusion polypeptide that has at least one activity of an IDI polypeptide. Exemplary IDI polypeptides and nucleic acids include naturally-occurring polypeptides and nucleic acids from any of the source organisms described herein as well as mutant polypeptides and nucleic acids derived from any of the source organisms described herein.
Acetoacetyl-CoA Synthase Gene
In another aspect, acetoacetyl-CoA synthase gene (aka nphT7) can be used. The acetoacetyl-CoA synthase gene is a gene encoding an enzyme having the activity of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and having minimal activity (e.g., no activity) of synthesizing acetoacetyl-CoA from two acetyl-CoA molecules. See, e.g., Okamura et al., PNAS Vol 107, No. 25, pp. 11265-11270 (2010), the contents of which are expressly incorporated herein for teaching about nphT7. An acetoacetyl-CoA synthase gene from an actinomycete of the genus Streptomyces CL190 strain was described in JP Patent Publication (Kokai) No. 2008-61506 A and US2010/0285549. Acetoacetyl-CoA synthase can also be referred to as acetyl CoA:malonyl CoA acyltransferase. A representative acetoacetyl-CoA synthase (or acetyl CoA:malonyl CoA acyltransferase) that can be used is Genbank AB540131.1.
In any of the aspects or embodiments described herein, an enzyme that has the ability to synthesize acetoacetyl-CoA from malonyl-CoA and acetyl-CoA can be used. In certain embodiments described herein, an acetoacetyl-CoA synthase gene derived from an actinomycete of the genus Streptomyces having the activity of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA can be used.
Exemplary Host Cells
A variety of host cells can be used to make a recombinant host cell that can express isoprene synthase variants and to produce isoprene in the methods of the claimed invention. The host cell may be a cell that naturally produces isoprene or a cell that does not naturally produce isoprene. In some embodiments, the host cell naturally produces isoprene using the DXP pathway, and an isoprene synthase variant, DXP pathway polypeptide (e.g., DXS), and/or IDI nucleic acid is added to enhance production of isoprene using this pathway. In some embodiments, the host cell naturally produces isoprene using the MVA pathway, and an isoprene synthase variant and/or one or more MVA pathway nucleic acids are added to enhance production of isoprene using this pathway. In some embodiments, the host cell naturally produces isoprene using the DXP pathway and one or more MVA pathway nucleic acids are added to produce isoprene using part or all of the MVA pathway as well as the DXP pathway. In some embodiments, the host cell naturally produces isoprene using both the DXP and MVA pathways and one or more isoprene synthase variants, DXS, IDI, or MVA pathway nucleic acids are added to enhance production of isoprene by one or both of these pathways.
In some embodiments, the host cell is a yeast, such as Saccharomyces sp., Schizosaccharomyces sp., Pichia sp., Candida sp. or Y. lipolytica. 
In some embodiments, the host cell is a bacterium, such as strains of Bacillus such as B. lichenformis or B. subtilis, strains of Pantoea such as P. citrea, strains of Pseudomonas such as P. alcaligenes, strains of Streptomyces such as S. lividans or S. rubiginosus, strains of Escherichia such as E. coli, strains of Enterobacter, strains of Streptococcus, strains of Archaea such as Methanosarcina mazei or strains of Corynebacterium such as C. glutamicum. 
As used herein, “the genus Bacillus” includes all species within the genus “Bacillus,” as known to those of skill in the art, including but not limited to B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans, B. lautus, and B. thuringiensis. It is recognized that the genus Bacillus continues to undergo taxonomical reorganization. Thus, it is intended that the genus include species that have been reclassified, including but not limited to such organisms as B. stearothermophilus, which is now named “Geobacillus stearothermophilus.” The production of resistant endospores in the presence of oxygen is considered the defining feature of the genus Bacillus, although this characteristic also applies to the recently named Alicyclobacillus, Amphibacillus, Aneurinibacillus, Anoxybacillus, Brevibacillus, Filobacillus, Gracilibacillus, Halobacillus, Paenibacillus, Salibacillus, Thermobacillus, Ureibacillus, and Virgibacillus. 
In some embodiments, the host cell is a gram-positive bacterium. Non-limiting examples include strains of Streptomyces (e.g., S. lividans, S. coelicolor, or S. griseus) and Bacillus. In some embodiments, the source organism is a gram-negative bacterium, such as E. coli or Pseudomonas sp.
In some embodiments, the host cell is a plant, such as a plant from the family Fabaceae, such as the Faboideae subfamily. In some embodiments, the source organism is kudzu, poplar (such as Populus alba×tremula CAC35696), aspen (such as Populus tremuloides), or Quercus robur. 
In some embodiments, the host cell is an algae, such as a green algae, red algae, glaucophytes, chlorarachniophytes, euglenids, chromista, or dinoflagellates.
In some embodiments, the host cell is a cyanobacteria, such as cyanobacteria classified into any of the following groups based on morphology: Chroococcales, Pleurocapsales, Oscillatoriales, Nostocales, or Stigonematales. 
In some embodiments, the host cell is an anaerobic organisms. An “anaerobe” is an organism that does not require oxygen for growth. An anaerobe can be an obligate anaerobe, a facultative anaerobe, or an aerotolerant organism. Such organisms can be any of the organisms listed above, bacteria, yeast, etc. An “obligate anaerobe” is an anaerobe for which atmospheric levels of oxygen can be lethal. Examples of obligate anaerobes include, but are not limited to, Clostridium, Eurobacterium, Bacteroides, Peptostreptococcus, Butyribacterium, Veillonella, and Actinomyces. In one embodiment, the obligate anaerobes can be any one or combination selected from the group consisting of Clostridium ljungdahlii, Clostridium autoethanogenum, Eurobacterium limosum, Clostridium carboxydivorans, Peptostreptococcus productus, and Butyribacterium methylotrophicum. A “facultative anaerobe” is an anaerobe that is capable of performing aerobic respiration in the presence of oxygen and is capable of performing anaerobic fermentation under oxygen-limited or oxygen-free conditions. Examples of facultative anaerobes include, but are not limited to, Escherichia, Pantoea, yeast, and Yarrowia. 
In some embodiments, the host cell is a photosynthetic cell. In other embodiments, the host cell is a non-photosynthetic cell.
Other exemplary host cells that can be used are described in US Pub. 2009/0203102, WO 2009/076676, WO 2010/003007, WO 2009/132220, WO 2010/031062, WO 2010/031068, WO 2010/031076, WO 2010/031077, and WO 2010/031079.
Exemplary Transformation Methods
Isoprene synthase, DXS, IDI, and/or MVA pathway nucleic acids or vectors containing them can be inserted into a host cell (e.g., a bacterial cell) using standard techniques for expression of the encoded isoprene synthase, DXS, IDI, and/or MVA pathway polypeptide. Introduction of a DNA construct or vector into a host cell can be performed using techniques such as transformation, electroporation, nuclear microinjection, transduction, transfection (e.g., lipofection mediated or DEAE-Dextrin mediated transfection or transfection using a recombinant phage virus), incubation with calcium phosphate DNA precipitate, high velocity bombardment with DNA-coated microprojectiles, and protoplast fusion. General transformation techniques are known in the art (see, e.g., Current Protocols in Molecular Biology (F. M. Ausubel et al. (eds) Chapter 9, 1987; Sambrook et al., Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor, 2001; and Campbell et al., Curr Genet, 16:53-56, 1989, which are each hereby incorporated by reference in their entireties, particularly with respect to transformation methods). The introduced nucleic acids may be integrated into chromosomal DNA or maintained as extrachromosomal replicating sequences.
Other exemplary transformation methods that can be used are described in US Pub. 2009/0203102, WO 2009/076676, WO 2010/003007, WO 2009/132220, WO 2010/031062, WO 2010/031068, WO 2010/031076, WO 2010/031077, and WO 2010/031079.
Exemplary Cell Culture Media
Any carbon source can be used to cultivate the host cells. The term “carbon source” refers to one or more carbon-containing compounds capable of being metabolized by a host cell or organism. For example, the cell medium used to cultivate the host cells may include any carbon source suitable for maintaining the viability or growing the host cells.
In some embodiments, the carbon source is a carbohydrate (such as monosaccharide, disaccharide, oligosaccharide, or polysaccharides), invert sugar (e.g., enzymatically treated sucrose syrup), glycerol, glycerine (e.g., a glycerine byproduct of a biodiesel or soap-making process), dihydroxyacetone, one-carbon source, fatty acid (e.g., a saturated fatty acid, unsaturated fatty acid, or polyunsaturated fatty acid), lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, polypeptide (e.g., a microbial or plant protein or peptide), renewable carbon source (e.g., a biomass carbon source such as a hydrolyzed biomass carbon source; beet sugar or cane sugar molasses), yeast extract, component from a yeast extract, polymer, acid, alcohol, aldehyde, ketone, amino acid, succinate, lactate, acetate, ethanol, or any combination of two or more of the foregoing. In some embodiments, the carbon source is a product of photosynthesis, including, but not limited to, glucose.
Exemplary monosaccharides include glucose and fructose; exemplary oligosaccharides include lactose and sucrose, and exemplary polysaccharides include starch and cellulose. Exemplary carbohydrates include C6 sugars (e.g., fructose, mannose, galactose, or glucose) and C5 sugars (e.g., xylose or arabinose). In some embodiments, the cell medium includes a carbohydrate as well as a carbon source other than a carbohydrate (e.g., glycerol, glycerine, dihydroxyacetone, one-carbon source, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, or a component from a yeast extract). In some embodiments, the cell medium includes a carbohydrate as well as a polypeptide (e.g., a microbial or plant protein or peptide). In some embodiments, the microbial polypeptide is a polypeptide from yeast or bacteria. In some embodiments, the plant polypeptide is a polypeptide from soy, corn, canola, jatropha, palm, peanut, sunflower, coconut, mustard, rapeseed, cottonseed, palm kernel, olive, safflower, sesame, or linseed.
In some embodiments, the concentration of the carbohydrate is at least or about 5 grams per liter of broth (g/L, wherein the volume of broth includes both the volume of the cell medium and the volume of the cells), such as at least or about 10, 15, 20, 30, 40, 50, 60, 80, 100, 150, 200, 300, 400, or more g/L. In some embodiments, the concentration of the carbohydrate is between about 50 and about 400 g/L, such as between about 100 and about 360 g/L, between about 120 and about 360 g/L, or between about 200 and about 300 g/L. In some embodiments, this concentration of carbohydrate includes the total amount of carbohydrate that is added before and/or during the culturing of the host cells.
Exemplary lipids are any substance containing one or more fatty acids that are C4 and above fatty acids that are saturated, unsaturated, or branched.
Exemplary fatty acids include compounds of the formula R—COOH, where “R” is a hydrocarbon. Exemplary unsaturated fatty acids include compounds where “R” includes at least one carbon-carbon double bond. Exemplary unsaturated fatty acids include, but are not limited to, oleic acid, vaccenic acid, linoleic acid, palmitelaidic acid, and arachidonic acid. Exemplary polyunsaturated fatty acids include compounds where “R” includes a plurality of carbon-carbon double bonds. Exemplary saturated fatty acids include compounds where “R” is a saturated aliphatic group. In some embodiments, the carbon source includes one or more C12-C22 fatty acids, such as a C12 saturated fatty acid, a C14 saturated fatty acid, a C16 saturated fatty acid, a C18 saturated fatty acid, a C20 saturated fatty acid, or a C22 saturated fatty acid. In an exemplary embodiment, the fatty acid is palmitic acid. In some embodiments, the carbon source is a salt of a fatty acid (e.g., an unsaturated fatty acid), a derivative of a fatty acid (e.g., an unsaturated fatty acid), or a salt of a derivative of fatty acid (e.g., an unsaturated fatty acid). Suitable salts include, but are not limited to, lithium salts, potassium salts, sodium salts, and the like. Di- and triglycerols are fatty acid esters of glycerol.
In some embodiments, the concentration of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride is at least or about 1 gram per liter of broth (g/L, wherein the volume of broth includes both the volume of the cell medium and the volume of the cells), such as at least or about 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, 150, 200, 300, 400, or more g/L. In some embodiments, the concentration of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride is between about 10 and about 400 g/L, such as between about 25 and about 300 g/L, between about 60 and about 180 g/L, or between about 75 and about 150 g/L. In some embodiments, the concentration includes the total amount of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride that is added before and/or during the culturing of the host cells. In some embodiments, the carbon source includes both (i) a lipid, fatty acid, monoglyceride, diglyceride, or triglyceride and (ii) a carbohydrate, such as glucose. In some embodiments, the ratio of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride to the carbohydrate is about 1:1 on a carbon basis (i.e., one carbon in the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride per carbohydrate carbon). In particular embodiments, the amount of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride is between about 60 and 180 g/L, and the amount of the carbohydrate is between about 120 and 360 g/L.
Exemplary microbial polypeptide carbon sources include one or more polypeptides from yeast or bacteria. Exemplary plant polypeptide carbon sources include one or more polypeptides from soy, corn, canola, jatropha, palm, peanut, sunflower, coconut, mustard, rapeseed, cottonseed, palm kernel, olive, safflower, sesame, or linseed.
Exemplary renewable carbon sources include cheese whey permeate, cornsteep liquor, sugar beet molasses, barley malt, and components from any of the foregoing. Exemplary renewable carbon sources also include glucose, hexose, pentose and xylose present in biomass, such as corn, switchgrass, sugar cane, cell waste of fermentation processes, and protein by-product from the milling of soy, corn, or wheat. In some embodiments, the biomass carbon source is a lignocellulosic, hemicellulosic, or cellulosic material such as, but are not limited to, a grass, wheat, wheat straw, bagasse, sugar cane bagasse, soft wood pulp, corn, corn cob or husk, corn kernel, fiber from corn kernels, corn stover, switch grass, rice hull product, or a by-product from wet or dry milling of grains (e.g., corn, sorghum, rye, triticate, barley, wheat, and/or distillers grains). Exemplary cellulosic materials include wood, paper and pulp waste, herbaceous plants, and fruit pulp. In some embodiments, the carbon source includes any plant part, such as stems, grains, roots, or tubers. In some embodiments, all or part of any of the following plants are used as a carbon source: corn, wheat, rye, sorghum, triticate, rice, millet, barley, cassava, legumes, such as beans and peas, potatoes, sweet potatoes, bananas, sugarcane, and/or tapioca. In some embodiments, the carbon source is a biomass hydrolysate, such as a biomass hydrolysate that includes both xylose and glucose or that includes both sucrose and glucose.
In some embodiments, the renewable carbon source (such as biomass) is pretreated before it is added to the cell culture medium. In some embodiments, the pretreatment includes enzymatic pretreatment, chemical pretreatment, or a combination of both enzymatic and chemical pretreatment (see, for example, Farzaneh et al., Bioresource Technology 96 (18): 2014-2018, 2005; U.S. Pat. No. 6,176,176; U.S. Pat. No. 6,106,888). In some embodiments, the renewable carbon source is partially or completely hydrolyzed before it is added to the cell culture medium.
In some embodiments, the renewable carbon source (such as corn stover) undergoes ammonia fiber expansion (AFEX) pretreatment before it is added to the cell culture medium (see, for example, Farzaneh et al., Bioresource Technology 96 (18): 2014-2018, 2005). During AFEX pretreatment, a renewable carbon source is treated with liquid anhydrous ammonia at moderate temperatures (such as about 60 to about 100° C.) and high pressure (such as about 250 to about 300 psi) for about 5 minutes. Then, the pressure is rapidly released. In this process, the combined chemical and physical effects of lignin solubilization, hemicellulose hydrolysis, cellulose decrystallization, and increased surface area enables near complete enzymatic conversion of cellulose and hemicellulose to fermentable sugars. AFEX pretreatment has the advantage that nearly all of the ammonia can be recovered and reused, while the remaining serves as nitrogen source for microbes in downstream processes. Also, a wash stream is not required for AFEX pretreatment. Thus, dry matter recovery following the AFEX treatment is essentially 100%. AFEX is basically a dry-to-dry process. The treated renewable carbon source is stable for long periods and can be fed at very high solid loadings in enzymatic hydrolysis or fermentation processes. Cellulose and hemicellulose are well preserved in the AFEX process, with little or no degradation. There is no need for neutralization prior to the enzymatic hydrolysis of a renewable carbon source that has undergone AFEX pretreatment. Enzymatic hydrolysis of AFEX-treated carbon sources produces clean sugar streams for subsequent fermentation use.
In some embodiments, the concentration of the carbon source (e.g., a renewable carbon source) is equivalent to at least or about 0.1, 0.5, 1, 1.5 2, 3, 4, 5, 10, 15, 20, 30, 40, or 50% glucose (w/v). The equivalent amount of glucose can be determined by using standard HPLC methods with glucose as a reference to measure the amount of glucose generated from the carbon source. In some embodiments, the concentration of the carbon source (e.g., a renewable carbon source) is equivalent to between about 0.1 and about 20% glucose, such as between about 0.1 and about 10% glucose, between about 0.5 and about 10% glucose, between about 1 and about 10% glucose, between about 1 and about 5% glucose, or between about 1 and about 2% glucose.
In some embodiments, the carbon source includes yeast extract or one or more components of yeast extract. In some embodiments, the concentration of yeast extract is at least 1 gram of yeast extract per liter of broth (g/L, wherein the volume of broth includes both the volume of the cell medium and the volume of the cells), such at least or about 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, 150, 200, 300, or more g/L. In some embodiments, the concentration of yeast extract is between about 1 and about 300 g/L, such as between about 1 and about 200 g/L, between about 5 and about 200 g/L, between about 5 and about 100 g/L, or between about 5 and about 60 g/L. In some embodiments, the concentration includes the total amount of yeast extract that is added before and/or during the culturing of the host cells. In some embodiments, the carbon source includes both yeast extract (or one or more components thereof) and another carbon source, such as glucose. In some embodiments, the ratio of yeast extract to the other carbon source is about 1:5, about 1:10, or about 1:20 (w/w).
Additionally the carbon source may also be one-carbon substrates such as carbon dioxide, or methanol. Glycerol production from single carbon sources (e.g., methanol, formaldehyde, or formate) has been reported in methylotrophic yeasts (Yamada et al., Agric. Biol. Chem., 53(2) 541-543, 1989) and in bacteria (Hunter et. al., Biochemistry, 24, 4148-4155, 1985). These organisms can assimilate single carbon compounds, ranging in oxidation state from methane to formate, and produce glycerol. The pathway of carbon assimilation can be through ribulose monophosphate, through serine, or through xylulose-momophosphate (Gottschalk, Bacterial Metabolism, Second Edition, Springer-Verlag: New York, 1986, which is hereby incorporated by reference in its entirety, particularly with respect to carbon sources). The ribulose monophosphate pathway involves the condensation of formate with ribulose-5-phosphate to form a six carbon sugar that becomes fructose and eventually the three carbon product glyceraldehyde-3-phosphate. Likewise, the serine pathway assimilates the one-carbon compound into the glycolytic pathway via methylenetetrahydrofolate.
In addition to one and two carbon substrates, methylotrophic organisms are also known to utilize a number of other carbon containing compounds such as methylamine, glucosamine and a variety of amino acids for metabolic activity. For example, methylotrophic yeast are known to utilize the carbon from methylamine to form trehalose or glycerol (Bellion et al., Microb. Growth Cl Compd., Int. Symp., 7th ed., 415-32. Editors: Murrell et al., Publisher: Intercept, Andover, UK, 1993). Similarly, various species of Candida metabolize alanine or oleic acid (Sulter et al., Arch. Microbiol. 153(5), 485-9, 1990).
In some embodiments, cells are cultured in a standard medium containing physiological salts and nutrients (see, e.g., Pourquie, J. et al., Biochemistry and Genetics of Cellulose Degradation, eds. Aubert et al., Academic Press, pp. 71-86, 1988; and Ilmen et al., Appl. Environ. Microbiol. 63:1298-1306, 1997). Exemplary growth media are common commercially prepared media such as Luria Bertani (LB) broth, Sabouraud Dextrose (SD) broth, or Yeast medium (YM) broth. One skilled in the art of microbiology or fermentation science would know other defined or synthetic growth media that may also be used, and the appropriate medium for growth of particular host cells.
In addition to an appropriate carbon source, the cell medium desirably contains suitable minerals, salts, cofactors, buffers, and other components known to those skilled in the art suitable for the growth of the cultures or the enhancement of isoprene production (see, for example, WO 2004/033646 and references cited therein and WO 96/35796 and references cited therein). In some embodiments where an isoprene synthase, DXS, IDI, and/or MVA pathway nucleic acid is under the control of an inducible promoter, the inducing agent (e.g., a sugar, metal salt or antimicrobial), is desirably added to the medium at a concentration effective to induce expression of an isoprene synthase, DXS, IDI, and/or MVA pathway polypeptide. In some embodiments, cell medium has an antibiotic (such as kanamycin) that corresponds to the antibiotic resistance nucleic acid (such as a kanamycin resistance nucleic acid) on a vector that has one or more DXS, IDI, or MVA pathway nucleic acids.
Other exemplary cell culture media that can be used are described in US Pub. 2009/0203102, WO 2009/076676, WO 2010/003007, WO 2009/132220, WO 2010/031062, WO 2010/031068, WO 2010/031076, WO 2010/031077, and WO 2010/031079.
Exemplary Production of Isoprene
In some embodiments, the cells are cultured in a culture medium under conditions permitting the production of isoprene by the cells. In some embodiments, the cells in culture produce isoprene at greater than or about 1, 10, 25, 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,250, 1,500, 1,750, 2,000, 2,500, 3,000, 4,000, 5,000, or more nmole of isoprene/gram of cells for the wet weight of the cells/hour (nmole/gwcm/hr). In some embodiments, the amount of isoprene is between about 2 to about 5,000 nmole/gwcm/hr, such as between about 2 to about 100 nmole/gwcm/hr, about 100 to about 500 nmole/gwcm/hr, about 150 to about 500 nmole/gwcm/hr, about 500 to about 1,000 nmole/gwcm/hr, about 1,000 to about 2,000 nmole/gwcm/hr, or about 2,000 to about 5,000 nmole/gwcm/hr. The amount of isoprene in units of nmole/gwcm/hr can be measured as disclosed in U.S. Pat. No. 5,849,970. For example, two mL of headspace (e.g., headspace from a culture such as 2 mL of culture cultured in sealed vials at 32° C. with shaking at 200 rpm for approximately 3 hours) are analyzed for isoprene using a standard gas chromatography system, such as a system operated isothermally (85° C.) with an n-octane/porasil C column (Alltech Associates, Inc., Deerfield, Ill.) and coupled to a RGD2 mercuric oxide reduction gas detector (Trace Analytical, Menlo Park, Calif.) (see, e.g., Greenberg et al, Atmos. Environ. 27A: 2689-2692, 1993; Silver et al., Plant Physiol. 97:1588-1591, 1991). The gas chromatography area units are converted to nmol isoprene via a standard isoprene concentration calibration curve. In some embodiments, the value for the grams of cells for the wet weight of the cells is calculated by obtaining the A600 value for a sample of the cell culture, and then converting the A600 value to grams of cells based on a calibration curve of wet weights for cell cultures with a known A600 value. In some embodiments, the grams of the cells is estimated by assuming that one liter of broth (including cell medium and cells) with an A600 value of 1 has a wet cell weight of 1 gram. The value is also divided by the number of hours the culture has been incubating for, such as three hours.
In some embodiments, the cells in culture produce isoprene at greater than or about 1, 10, 25, 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,250, 1,500, 1,750, 2,000, 2,500, 3,000, 4,000, 5,000, 10,000, 100,000, or more ng of isoprene/gram of cells for the wet weight of the cells/hr (ng/gwcm/h). In some embodiments, the amount of isoprene is between about 2 to about 5,000 ng/gwcm/h, such as between about 2 to about 100 ng/gwcm/h, about 100 to about 500 ng/gwcm/h, about 500 to about 1,000 ng/gwcm/h, about 1,000 to about 2,000 ng/gwcm/h, or about 2,000 to about 5,000 ng/gwcm/h. The amount of isoprene in ng/gwcm/h can be calculated by multiplying the value for isoprene production in the units of nmole/gwcm/hr discussed above by 68.1 (as described in Equation 5 below).
In some embodiments, the cells in culture produce a cumulative titer (total amount) of isoprene at greater than or about 1, 10, 25, 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,250, 1,500, 1,750, 2,000, 2,500, 3,000, 4,000, 5,000, 10,000, 50,000, 100,000, or more mg of isoprene/L of broth (mg/L broth, wherein the volume of broth includes the volume of the cells and the cell medium). In some embodiments, the amount of isoprene is between about 2 to about 5,000 mg/L broth, such as between about 2 to about 100 mg/L broth, about 100 to about 500 mg/L broth, about 500 to about 1,000 mg/L broth, about 1,000 to about 2,000 mg/L broth, or about 2,000 to about 5,000 mg/L broth. The specific productivity of isoprene in mg of isoprene/L of headspace from shake flask or similar cultures can be measured by taking a 1 ml sample from the cell culture at an OD600 value of approximately 1.0, putting it in a 20 mL vial, incubating for 30 minutes, and then measuring the amount of isoprene in the headspace. If the OD600 value is not 1.0, then the measurement can be normalized to an OD600 value of 1.0 by dividing by the OD600 value. The value of mg isoprene/L headspace can be converted to mg/Lbroth/hr/OD600 of culture broth by multiplying by a factor of 38. The value in units of mg/Lbroth/hr/OD600 can be multiplied by the number of hours and the OD600 value to obtain the cumulative titer in units of mg of isoprene/L of broth.
The instantaneous isoprene production rate in mg/L broth/hr in a fermentor can be measured by taking a sample of the fermentor off-gas, analyzing it for the amount of isoprene (in units such as mg of isoprene per L of gas), and multiplying this value by the rate at which off-gas is passed though each liter of broth (e.g., at 1 vvm (volume of air/volume of broth/minute) this is 60 Lgas per hour). Thus, an off-gas level of 1 mg/Lgas corresponds to an instantaneous production rate of 60 mg/Lbroth/hr at air flow of 1 vvm. If desired, the value in the units mg/Lbroth/hr can be divided by the OD600 value to obtain the specific rate in units of mg/Lbroth/hr/OD. The average value of mg isoprene/Lgas can be converted to the total product productivity (grams of isoprene per liter of fermentation broth, mg/Lbroth) by multiplying this average off-gas isoprene concentration by the total amount of off-gas sparged per liter of fermentation broth during the fermentation. Thus, an average off-gas isoprene concentration of 0.5 mg/Lbroth/hr over 10 hours at 1 vvm corresponds to a total product concentration of 300 mg isoprene/Lbroth.
In some embodiments, the cells in culture convert greater than or about 0.0015, 0.002, 0.005, 0.01, 0.02, 0.05, 0.1, 0.12, 0.14, 0.16, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.2, 1.4, or 1.6% of the carbon in the cell culture medium into isoprene. In some embodiments, the percent conversion of carbon into isoprene is between about 0.002 to about 1.6%, such as about 0.002 to about 0.005%, about 0.005 to about 0.01%, about 0.01 to about 0.05%, about 0.05 to about 0.15%, 0.15 to about 0.2%, about 0.2 to about 0.3%, about 0.3 to about 0.5%, about 0.5 to about 0.8%, about 0.8 to about 1.0%, or about 1.0 to about 1.6%. The percent conversion of carbon into isoprene (also referred to as “% carbon yield”) can be measured by dividing the moles carbon in the isoprene produced by the moles carbon in the carbon source (such as the moles of carbon in batched and fed glucose and yeast extract). This number is multiplied by 100% to give a percentage value (as indicated in Equation 1).

% Carbon Yield=(moles carbon in isoprene produced)/(moles carbon in carbon source)*100  Equation 1

For this calculation, yeast extract can be assumed to contain 50% w/w carbon.

% Carbon Yield=(39.1 g isoprene*1/68.1 mol/g*5 C/mol)/[(181221 g glucose*1/180 mol/g*6 C/mol)+(17780 g yeast extract*0.5*1/12 mol/g)]*100=0.042%  Equation 2

One skilled in the art can readily convert the rates of isoprene production or amount of isoprene produced into any other units. Exemplary equations are listed below for interconverting between units.
Units for Rate of Isoprene Production (Total and Specific)

1 g isoprene/Lbroth/hr=14.7 mmol isoprene/Lbroth/hr(total volumetric rate)  Equation 3

1 nmol isoprene/gwcm/hr=1 nmol isoprene/Lbroth/hr/OD600(This conversion assumes that one liter of broth with an OD600 value of 1 has a wet cell weight of 1 gram.)  Equation 4

1 nmol isoprene/gwcm/hr=68.1 ng isoprene/gwcm/hr(given the molecular weight of isoprene)  Equation 5

1 nmol isoprene/LgasO2/hr=90 nmol isoprene/Lbroth/hr(at an O2 flow rate of 90 L/hr per L of culture broth)  Equation 6

1 μg isoprene/Lgas isoprene in off-gas=60 μg isoprene/Lbroth/hr at a flow rate of 60 Lgas per Lbroth(1 vvm)  Equation 7

Units for Titer (Total and Specific)

1 nmol isoprene/mg cell protein=150 nmol isoprene/Lbroth/OD600(This conversion assumes that one liter of broth with an OD600 value of 1 has a total cell protein of approximately 150 mg)(specific productivity)  Equation 8

1 g isoprene/Lbroth=14.7 mmol isoprene/Lbroth(total titer)  Equation 9

If desired, Equation 10 can be used to convert any of the units that include the wet weight of the cells into the corresponding units that include the dry weight of the cells.

Dry weight of cells=(wet weight of cells)/3.3  Equation 10

In some embodiments encompassed by the invention, a cell comprising a heterologous nucleic acid encoding an isoprene synthase variant polypeptide produces an amount of isoprene that is at least or about 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold, 100-fold, 150-fold, 200-fold, 400-fold, or greater than the amount of isoprene produced from a corresponding cell grown under essentially the same conditions without the heterologous nucleic acid encoding the isoprene synthase variant polypeptide.
In some embodiments encompassed by the invention, a cell comprising a heterologous nucleic acid encoding an isoprene synthase variant polypeptide and one or more heterologous nucleic acids encoding a DXS, IDI, and/or MVA pathway polypeptide produces an amount of isoprene that is at least or about 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold, 100-fold, 150-fold, 200-fold, 400-fold, or greater than the amount of isoprene produced from a corresponding cell grown under essentially the same conditions without the heterologous nucleic acids.
Exemplary Isoprene Purification Methods
In some embodiments, any of the methods described herein further include recovering the isoprene. For example, the isoprene produced using the compositions and methods of the invention can be recovered using standard techniques, such as gas stripping, fractionation, adsorption/desorption, pervaporation, thermal or vacuum desorption of isoprene from a solid phase, or extraction of isoprene immobilized or absorbed to a solid phase with a solvent (see, for example, U.S. Pat. Nos. 4,703,007 and 4,570,029). In some embodiments, the recovery of isoprene involves the isolation of isoprene in a liquid form (such as a neat solution of isoprene or a solution of isoprene in a solvent). Gas stripping involves the removal of isoprene vapor from the fermentation off-gas stream in a continuous manner. Such removal can be achieved in several different ways including, but not limited to, adsorption to a solid phase, partition into a liquid phase, or direct condensation. In some embodiments, membrane enrichment of a dilute isoprene vapor stream above the dew point of the vapor resulting in the condensation of liquid isoprene. In some embodiments, the recovery is performed as described in U.S. Provisional Patent Appl. No. 61/288,142, filed on Dec. 18, 2009.
The recovery of isoprene may involve one step or multiple steps. In some embodiments, the removal of isoprene vapor from the fermentation off-gas and the conversion of isoprene to a liquid phase are performed simultaneously. For example, isoprene can be directly condensed from the off-gas stream to form a liquid. In some embodiments, the removal of isoprene vapor from the fermentation off-gas and the conversion of isoprene to a liquid phase are performed sequentially. For example, isoprene may be adsorbed to a solid phase and then extracted from the solid phase with a solvent.
In some embodiments, any of the methods described herein further include purifying the isoprene. For example, the isoprene produced using the compositions and methods of the invention can be purified using standard techniques. Purification refers to a process through which isoprene is separated from one or more components that are present when the isoprene is produced. In some embodiments, the isoprene is obtained as a substantially pure liquid. Examples of purification methods include (i) distillation from a solution in a liquid extractant and (ii) chromatography. As used herein, “purified isoprene” means isoprene that has been separated from one or more components that are present when the isoprene is produced. See, e.g. U.S. Patent Application Publication No. 2009/0203102, PCT publication WO 2009/076676 and U.S. patent application Ser. No. 12/496,573. In some embodiments, the isoprene is at least about 20%, by weight, free from other components that are present when the isoprene is produced. In various embodiments, the isoprene is at least or about 25%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 90%, 95%, or 99%, by weight, pure. Purity can be assayed by any appropriate method, e.g., by column chromatography, HPLC analysis, or GC-MS analysis.
All publications and patents mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. It is also to be understood that one, some, or all of the properties of the various embodiments described herein may be combined to form other embodiments of the present invention. These and other aspects of the invention will become apparent to one of skill in the art.
EXAMPLES
A pool of mutants located near the active site of MEA P. alba was screened for the ability to convert DMAPP to isoprene and confer improved growth to the host expression strain. After several rounds of enrichment, the S288C mutation was the predominant variant in the mutant pool. S288C conferred significantly increased solubility to MEA P. alba when expressed in host cells. S288C conferred increased solubility to IspS during large scale fermentation, and also significantly increased the solubility of IspS enzymes derived from Salix alba and Salix babylonica willow species.
Example 1
Enrichment of a Pool of MEA P. alba Mutants Near the Enzyme Active Site Identified the S288C Mutation
A pool of mutants located near the active site of MEA P. alba was screened for the ability to convert DMAPP to isoprene and confer improved growth to the host expression strain.
Methods
The entire MEA P. alba IspS enzyme (544 positions) was mutagenized by QuikChange (Stratagene) PCR using NNK/MNN oligonucleotide primer pairs at every position. Substitution of NNK (32 possible codons) at each position in the coding sequence of MEA P. alba allowed for all 20 possible amino acids to be represented in mutant pools. NNK primers were systematically generated to have 10 bases upstream and 20 bases downstream of the codon to be mutagenized. The complementary MNN primers were systematically generated in the exact same way with a 23 base overlap between the primer pair. Quikchange PCR reactions were carried out according to the manufacturer's recommended protocol. Recombinant mutants were generated after transformation of the PCR products.
To identify individual variants of MEA P. alba IspS that allow for increased activity within the host, 51 positions within an approximate radius of 8 angstroms from the active site (PDB 3N0G) were selected for pooling (see Table 1 and FIG. 1). NNK PCR products from each of these positions were pooled and transformed into electrocompetent MD09-170 cells. Approximately 7,500 independent transformants were isolated and then pooled prior to the enrichment procedure. The 7,500 transformants represented an approximate 5-fold oversampling of the possible mutants at each position (32 codons for NNK at each position, 1,632 possible codons in a pool of 51 positions). This pool was then subjected to several rounds of enrichment by growth under MVA supplement and induction of IspS by IPTG as previously described in US 2010/0003716. After round three of enrichment, the IspS ORF in either 8 or 16 individual isolates was fully sequenced prior to the next round of enrichment. The enrichment procedure was stopped after round six.
Results
Enrichment of the pool of individual mutants in the 51 positions near the active site of MEA P. alba IspS (see Table 1) identified the S288C variant as the predominant mutation after 5 rounds of selection. Table 2 shows that 13 out of 16 sequenced variants randomly isolated after round 5 harbored the S288C mutation. S288C was not present in any of 8 isolated variants post earlier rounds 3 or 4, and was only present in 2 isolated variants (out of 16) after round 6. Table 2 also shows that after round 6, the predominant mutation was a silent R435R (CGT to CGG), which likely caused a simple IspS expression increase in the host strain. This indicates that in the enrichment procedure there is a narrow window for identification of variants that convey beneficial properties to IspS, prior to the proliferation of variants that simply improve expression.







TABLE 1







Positions near the enzyme active site located approximately 8 Å from


the active site as defined by the substrate analog and coordinated metals


in the PDB 3N0G.










Residue
Position














ARG
259



SER
263



TRP
266



MET
286



PHE
287



SER
288



PHE
289



VAL
290



THR
291



ILE
292



ILE
293



ASP
294



ASP
295



ILE
296



TYR
297



ASP
298



VAL
299



LEU
365



CYS
366



ALA
368



PHE
369



LEU
370



GLN
371



GLU
372



ALA
373



ASN
390



ALA
391



TRP
392



LYS
393



SER
394



SER
395



SER
396



GLY
397



GLN
400



PHE
434



ARG
435



LEU
436



CYS
437



ASN
438



ASP
439



LEU
440



ALA
441



SER
442



ALA
443



GLU
446



GLU
451



THR
452



ALA
453



ASN
454



SER
455



SER
510

















TABLE 2







Variants isolated in each round of enrichment of active site pool. Isolates


that did not produce clear sequencing results are labeled “ND.”












Round 3
Round 4
Round 5
Round 6















Isolate 1
A368T
T452H
S288C
R435R (cgt-cgg)


Isolate 2
T452Y
N390W
S288C
R435R (cgt-cgg)


Isolate 3
A368T
A391A (gca-gct)
S288C
V299L


Isolate 4
C437Q
T452E
ND
R435R (cgt-cgg)


Isolate 5
V299V
N390W
S288C
R435R (cgt-cgg)



(gta-gtt)


Isolate 6
A368N
R435R (cgt-cgg)
S288C
R435R (cgt-cgg)


Isolate 7
T452S
N390W
S288C
R435R (cgt-cgg)


Isolate 8
ND
ND
S288C
R435R (cgt-cgg)


Isolate 9


S288C
V299L


Isolate 10


S288C
S288C


Isolate 11


S288C
R435R (cgt-cgg)


Isolate 12


S288C
R435R (cgt-cgg)


Isolate 13


S288C
S288C


Isolate 14


A443G
R435R (cgt-cgg)


Isolate 15


V299L
I292L


Isolate 16


S288C
V299L









Example 2
The S288C Variant Confers Solubility to MEA P. alba Isoprene Synthase
A pool of mutants located near the active site of MEA P. alba was screened for the ability to convert DMAPP to isoprene and confer improved growth to the host expression strain. Since S288C was the predominant mutation after several rounds of enrichment (described in Example 1), and conveyed a clear benefit to intracellular activity of IspS, various strains harboring the mutation were isolated for further analysis.
Methods
To examine solubility of the MEA P. alba IspS S288C variant in comparison to wild type, strain DW425 containing the pCL201 plasmid encoding wild type IspS and strain DW526 containing the pDW218 plasmid encoding the S288C variant (FIG. 2) were inoculated into LB medium, grown, and induced with IPTG according to standard molecular biology practices (See Table 5 and Table 6). The S288C mutation was introduced into pCHL243 (FIG. 3), which contains the G491S variant of isoprene synthase, by the QuikChange procedure described above using primers shown in Table 4. The pCHL243 was generated by introduction of the G491S mutation into pDW34 (see below for pDW34 generation), which encodes the wild-type isoprene synthase, by QuikChange (Stratagene) mutagenesis (see below for PCR cycling parameters).
PCR Mixture for QuikChange Reaction:














Template DNA (pTrc-MEA-Alba-
~100
ng


mMVK)


G507S QC 2 For
50
uM


G507S QC 2 Rev
50
uM


dNTPs (Roche)
1
ul


10X pfu II fusion buffer
5
ul


Pfu Ultra II
1
ul


Water
add enough to reach 50
ul total









PCR Cycling Parameters for QuikChange Reaction:

95° C.—1 minute

(95° C. 50 seconds, 60° C. 50 seconds, 68° C. 3 minutes) 18 rounds

68° C.—10 minutes








TABLE 3





Primers
















G507S QC 2 For
GAAAAACTGAGTGGTAGCCTGTTCGCGAAAC


 


G507S QC 2 Rev
AGGCTACCACTCAGTTTTTCCTTGTTCATCT









The resultant plasmid from introduction of S288C into pCHL243 was termed pDW161 and was confirmed by sequencing to harbor the MEA P. alba S288C G491S variant of IspS (See FIG. 4 and Table 4). Strain DW532 was generated by co-transformation of plasmid pMCM82 and pDW161 into strain CMP451 using standard molecular biology practices (Table 6). Positive strains resistant to both carbenicillin and spectinomycin were selected for fermentation at the 14 L scale. Samples from either shake flask experiments or fermentation were subjected to centrifugation and frozen at −80° C. prior to analysis.
Frozen samples were thawed and resuspended in lysis buffer (100 mM Tris, 100 mM NaCl pH 7.6 buffer, 0.1 mg/ml DNase, 0.5 mM PMSF/AEBSF, 5 mM MgCl2) and passed through a French pressure cell as previously described. Lysed cultures were then spun at full speed in a table-top microcentrifuge for 10 minutes at 4° C. The supernatants were removed, and the pellets were washed 1× in lysis buffer before a second centrifugation step in the microcentrifuge. Samples were removed from the centrifuge, and pellets were resuspended in lysis buffer to exactly the same volume as the supernatant from the first centrifugation step. Equivalent volumes were then treated with loading buffer (Invitrogen) and run on Coomassie gels according to the manufacturer's recommended protocol (Invitrogen, NuPage). Proteins that were soluble were present in the lane carrying samples from supernatants, and insoluble proteins were present in the lanes carrying samples from resuspended pellets. Samples from fermentation strains CMP563 (see Table 6) and DW532 were also fractionated following the procedure described above, in order to observe the effect of the S288C variant at large scale. Soluble and insoluble IspS fractions from fermentation samples were detected by Western blot analysis (Invitrogen, WesternBreeze) and quantification using a Storm 860 Molecular Imager (GMI, Inc.), as previously described and according to the manufacturer's recommended protocol. Generation of plasmids pDW34 and pMCM82, and strains CMP563, DW532, and CMP451 was previously described in WO2009/076676, WO2010/003007, WO2009132220, WO2010/031062, WO2010/031068, WO2010/031076, WOWO2010/031077, WO2010/031079, WO2010/148150, WO2010/005525, WO2010/078457, WO2010/124146, WO2011/075534, WO2010/148144, WO2011/079314, WO2011/075748, and WO2002/010398 all incorporated herein for reference.







TABLE 4







Primers used for QuikChange mutagenesis








Primer
Sequence





HgS QC S288C
AAAAATGTTTTGTTTCGTAACCATTATCGACGA


Forward



 


HgS QC S288C
TGGTTACGAAACAAAACATTTTTGCGACGGAGT


Reverse
















TABLE 5







Plasmids used in this study











Plasmid
Resistance
Description







pDW218
Kan
pET MEA P. alba IspS S288C



pDW161
Carb
pTrc MEA P. alba IspS G491S S288C

















TABLE 6







Strains used in this study









Strain
Plasmid(s)
Description





DW425
pCL201
BL21 (DE3) PL.2-mKKDyI, pET MEA




P. alba IspS (control)


DW526
pDW218
BL21 (DE3) PL.2-mKKDyI, pET MEA P. alba




IspS S288C


CMP563
pMCM82,
BL21 pgl+ PL.2 mKKDyI GI1.2 gltA,



pCHL243
pTrc-MEA P. alba (G491S)-mMVK,




MCM82 (pCL-Upper MVA pathway)


DW532
pMCM82,
BL21 pgl+ PL.2 mKKDyI GI1.2 gltA,



pDW161
pTrc-MEA P. alba (S288C G491S)-mMVK,




MCM82 (pCL-Upper MVA pathway)









Results

Strain DW526 was isolated directly from the enrichment pool, induced and fractionated to determine the effect of S288C on solubility in comparison to wild type. FIG. 5 shows that the MEA P. alba IspS S288C variant is absent from the insoluble fraction compared to the control molecule (lane 2 compared to lane 4 of the Coomassie gel). FIG. 5 also shows that the control IspS enzyme was present in the soluble and insoluble fractions at an approximate ratio of 1:1, while the S288C was found exclusively in the soluble fraction. FIG. 6 shows the effect of the S288C mutation on the solubility of IspS in a larger scale fermentation experiment. In this study, the parental IspS enzyme harbored the G491S mutation, which previously was shown to have a positive effect on cell viability, yet no effect on solubility. In comparison to the parent enzyme, the IspS G491S S288C enzyme displayed significantly increased solubility in a fermentation strain. FIG. 6 shows that solubility of the S288C G491S molecule was increased to 90% of the total amount of IspS, in comparison to the G491S molecule alone, which was only 78% soluble.







Amino acid sequence of MEA P. alba IspS S288C


MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNE


 


KAEFLTLLELIDNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLH


 


GTALSFRLLRQHGFEVSQEAFSGFKDQNGNFLENLKEDIKAILSLYEAS


 


FLALEGENILDEAKVFAISHLKELSEEKIGKELAEQVNHALELPLHRRT


 


QRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRETSRWWR


 


RVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFCFVTIID


 


DIYDVYGTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIA


 


YDNLKDKGENILPYLTKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAW


 


KSSSGPLQLVFAYFAVVQNIKKEEIENLQKYHDTISRPSHIFRLCNDLA


 


SASAEIARGETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKEKL


 


GGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPI


 


LPFER


 


DNA sequence of pDW218 harboring MEA P. alba


IspS S288C


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtg


 


gtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccg


 


ctcctttcgctttcttcccttcctttctcgccacgttcgccggctttcc


 


ccgtcaagctctaaatcgggggctccctttagggttccgatttagtgct


 


ttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgta


 


gtgggccatcgccctgatagacggtttttcgccctttgacgttggagtc


 


cacgttctttaatagtggactcttgttccaaactggaacaacactcaac


 


cctatctcggtctattcttttgatttataagggattttgccgatttcgg


 


cctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattt


 


taacaaaatattaacgtttacaatttcaggtggcacttttcggggaaat


 


gtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgt


 


atccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaa


 


actgcaatttattcatatcaggattatcaataccatatttttgaaaaag


 


ccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatg


 


gcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatac


 


aacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatc


 


accatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatt


 


tctttccagacttgttcaacaggccagccattacgctcgtcatcaaaat


 


cactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgag


 


acgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaa


 


tgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctg


 


aatcaggatattcttctaatacctggaatgctgttttcccggggatcgc


 


agtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatg


 


gtcggaagaggcataaattccgtcagccagtttagtctgaccatctcat


 


ctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactc


 


tggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgc


 


ccgacattatcgcgagcccatttatacccatataaatcagcatccatgt


 


tggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggct


 


cataacaccccttgtattactgtttatgtaagcagacagttttattgtt


 


catgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagac


 


cccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcg


 


taatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttg


 


tttgccggatcaagagctaccaactctttttccgaaggtaactggcttc


 


agcagagcgcagataccaaatactgtccttctagtgtagccgtagttag


 


gccaccacttcaagaactctgtagcaccgcctacatacctcgctctgct


 


aatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttacc


 


gggttggactcaagacgatagttaccggataaggcgcagcggtcgggct


 


gaacggggggttcgtgcacacagcccagcttggagcgaacgacctacac


 


cgaactgagatacctacagcgtgagctatgagaaagcgccacgcttccc


 


gaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacag


 


gagagcgcacgagggagcttccagggggaaacgcctggtatctttatag


 


tcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgc


 


tcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt


 


tacggttcctggccttttgctggccttttgctcacatgttctttcctgc


 


gttatcccctgattctgtggataaccgtattaccgcctttgagtgagct


 


gataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcg


 


aggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtg


 


cggtatttcacaccgcatatatggtgcactctcagtacaatctgctctg


 


atgccgcatagttaagccagtatacactccgctatcgctacgtgactgg


 


gtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgac


 


gggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctc


 


cgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcg


 


aggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacaga


 


tgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgt


 


taatgtctggcttctgataaagcgggccatgttaagggcggttttttcc


 


tgtttggtcactgatgcctccgtgtaagggggatttctgttcatggggg


 


taatgataccgatgaaacgagagaggatgctcacgatacgggttactga


 


tgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcg


 


gtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagc


 


gcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcc


 


tgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtt


 


tccagactttacgaaacacggaaaccgaagaccattcatgttgttgctc


 


aggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtat


 


cggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgg


 


gtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgc


 


cggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagt


 


gacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgac


 


aggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatga


 


cccagagcgctgccggcacctgtcctacgagttgcatgataaagaagac


 


agtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggag


 


ctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcct


 


aatgagtgagctaacttacattaattgcgttgcgctcactgcccgcttt


 


ccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgc


 


gcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttc


 


accagtgagacgggcaacagctgattgcccttcaccgcctggccctgag


 


agagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatc


 


ctgtttgatggtggttaacggcgggatataacatgagctgtcttcggta


 


tcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggact


 


cggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccag


 


catcgcagtgggaacgatgccctcattcagcatttgcatggtttgttga


 


aaaccggacatggcactccagtcgccttcccgttccgctatcggctgaa


 


tttgattgcgagtgagatatttatgccagccagccagacgcagacgcgc


 


cgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc


 


aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggaga


 


aaataatactgttgatgggtgtctggtcagagacatcaagaaataacgc


 


cggaacattagtgcaggcagcttccacagcaatggcatcctggtcatcc


 


agcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgt


 


gcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacac


 


caccacgctggcacccagttgatcggcgcgagatttaatcgccgcgaca


 


atttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatca


 


gcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgta


 


attcagctccgccatcgccgcttccactttttcccgcgttttcgcagaa


 


acgtggctggcctggttcaccacgcgggaaacggtctgataagagacac


 


cggcatactctgcgacatcgtataacgttactggtttcacattcaccac


 


cctgaattgactctcttccgggcgctatcatgccataccgcgaaaggtt


 


ttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgac


 


tcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccg


 


ccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtccccc


 


ggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagc


 


ccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatatagg


 


cgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtcc


 


ggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcacta


 


taggggaattgtgagcggataacaattcccctctagaaataattttgtt


 


taactttaagaaggagatatacatatggaagcacgtcgctctgcgaact


 


acgaacctaacagctgggactatgattacctgctgtcctccgacacgga


 


cgagtccatcgaagtatacaaagacaaagcgaaaaagctggaagccgaa


 


gttcgtcgcgagattaataacgaaaaagcagaatttctgaccctgctgg


 


aactgattgacaacgtccagcgcctgggcctgggttaccgtttcgagtc


 


tgatatccgtggtgcgctggatcgcttcgtttcctccggcggcttcgat


 


gcggtaaccaagacttccctgcacggtacggcactgtctttccgtctgc


 


tgcgtcaacacggttttgaggtttctcaggaagcgttcagcggcttcaa


 


agaccaaaacggcaacttcctggagaacctgaaggaagatatcaaagct


 


atcctgagcctgtacgaggccagcttcctggctctggaaggcgaaaaca


 


tcctggacgaggcgaaggttttcgcaatctctcatctgaaagaactgtc


 


tgaagaaaagatcggtaaagagctggcagaacaggtgaaccatgcactg


 


gaactgccactgcatcgccgtactcagcgtctggaagcagtatggtcta


 


tcgaggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagct


 


ggcaattctggattacaacatgatccagtctgtataccagcgtgatctg


 


cgtgaaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaactgc


 


actttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgt


 


agcattcgaaccgcaatactccgactgccgtaactccgtcgcaaaaatg


 


ttttgtttcgtaaccattatcgacgatatctacgatgtatacggcaccc


 


tggacgaactggagctgtttactgatgcagttgagcgttgggacgtaaa


 


cgccatcaacgacctgccggattacatgaaactgtgctttctggctctg


 


tataacactattaacgaaatcgcctacgacaacctgaaagataaaggtg


 


agaacatcctgccgtatctgaccaaagcctgggctgacctgtgcaacgc


 


tttcctgcaagaagccaagtggctgtacaacaaatctactccgaccttt


 


gacgactacttcggcaacgcatggaaatcctcttctggcccgctgcaac


 


tggtgttcgcttacttcgctgtcgtgcagaacattaaaaaggaagagat


 


cgaaaacctgcaaaaataccatgacaccatctctcgtccttcccatatc


 


ttccgtctgtgcaatgacctggctagcgcgtctgcggaaattgcgcgtg


 


gtgaaaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctc


 


cgaagaactggctaccgaaagcgtgatgaatctgatcgatgaaacctgg


 


aaaaagatgaacaaggaaaaactgggtggtagcctgttcgcgaaaccgt


 


tcgtggaaaccgcgatcaacctggcacgtcaatctcactgcacttatca


 


taacggcgacgcgcatacctctccggatgagctgacccgcaaacgcgtt


 


ctgtctgtaatcactgaaccgattctgccgtttgaacgctaaggatccg


 


aattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccacc


 


accaccactgagatccggctgctaacaaagcccgaaaggaagctgagtt


 


ggctgctgccaccgctgagcaataactagcataaccccttggggcctct


 


aaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat


 


DNA sequence of pCHL243 harboring MEA P. alba


IspS G491S


gtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtc


 


aggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactg


 


cataattcgtgtcgctcaaggcgcactcccgttctggataatgtttttt


 


gcgccgacatcataacggttctggcaaatattctgaaatgagctgttga


 


caattaatcatccggctcgtataatgtgtggaattgtgagcggataaca


 


atttcacacaggaaacagcgccgctgagaaaaagcgaagcggcactgct


 


ctttaacaatttatcagacaatctgtgtgggcactcgaccggaattatc


 


gattaactttattattaaaaattaaagaggtatatattaatgtatcgat


 


taaataaggaggaataaaccatggaagctcgtcgttctgcgaactacga


 


acctaacagctgggactatgattacctgctgtcctccgacacggacgag


 


tccatcgaagtatacaaagacaaagcgaaaaagctggaagccgaagttc


 


gtcgcgagattaataacgaaaaagcagaatttctgaccctgctggaact


 


gattgacaacgtccagcgcctgggcctgggttaccgtttcgagtctgat


 


atccgtggtgcgctggatcgcttcgtttcctccggcggcttcgatgcgg


 


taaccaagacttccctgcacggtacggcactgtctttccgtctgctgcg


 


tcaacacggttttgaggtttctcaggaagcgttcagcggcttcaaagac


 


caaaacggcaacttcctggagaacctgaaggaagatatcaaagctatcc


 


tgagcctgtacgaggccagcttcctggctctggaaggcgaaaacatcct


 


ggacgaggcgaaggttttcgcaatctctcatctgaaagaactgtctgaa


 


gaaaagatcggtaaagagctggcagaacaggtgaaccatgcactggaac


 


tgccactgcatcgccgtactcagcgtctggaagcagtatggtctatcga


 


ggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagctggca


 


attctggattacaacatgatccagtctgtataccagcgtgatctgcgtg


 


aaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaactgcactt


 


tgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagca


 


ttcgaaccgcaatactccgactgccgtaactccgtcgcaaaaatgtttt


 


ctttcgtaaccattatcgacgatatctacgatgtatacggcaccctgga


 


cgaactggagctgtttactgatgcagttgagcgttgggacgtaaacgcc


 


atcaacgacctgccggattacatgaaactgtgctttctggctctgtata


 


acactattaacgaaatcgcctacgacaacctgaaagataaaggtgagaa


 


catcctgccgtatctgaccaaagcctgggctgacctgtgcaacgctttc


 


ctgcaagaagccaagtggctgtacaacaaatctactccgacctttgacg


 


actacttcggcaacgcatggaaatcctcttctggcccgctgcaactggt


 


gttcgcttacttcgctgtcgtgcagaacattaaaaaggaagagatcgaa


 


aacctgcaaaaataccatgacaccatctctcgtccttcccatatcttcc


 


gtctgtgcaatgacctggctagcgcgtctgcggaaattgcgcgtggtga


 


aaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaa


 


gaactggctaccgaaagcgtgatgaatctgatcgatgaaacctggaaaa


 


agatgaacaaggaaaaactgagtggtagcctgttcgcgaaaccgttcgt


 


ggaaaccgcgatcaacctggcacgtcaatctcactgcacttatcataac


 


ggcgacgcgcatacctctccggatgagctgacccgcaaacgcgttctgt


 


ctgtaatcactgaaccgattctgccgtttgaacgctaactgcataaagg


 


aggtaaaaaaacatggtatcctgttctgcgccgggtaagatttacctgt


 


tcggtgaacacgccgtagtttatggcgaaactgcaattgcgtgtgcggt


 


ggaactgcgtacccgtgttcgcgcggaactcaatgactctatcactatt


 


cagagccagatcggccgcaccggtctggatttcgaaaagcacccttatg


 


tgtctgcggtaattgagaaaatgcgcaaatctattcctattaacggtgt


 


tttcttgaccgtcgattccgacatcccggtgggctccggtctgggtagc


 


agcgcagccgttactatcgcgtctattggtgcgctgaacgagctgttcg


 


gctttggcctcagcctgcaagaaatcgctaaactgggccacgaaatcga


 


aattaaagtacagggtgccgcgtccccaaccgatacgtatgtttctacc


 


ttcggcggcgtggttaccatcccggaacgtcgcaaactgaaaactccgg


 


actgcggcattgtgattggcgataccggcgttttctcctccaccaaaga


 


gttagtagctaacgtacgtcagctgcgcgaaagctacccggatttgatc


 


gaaccgctgatgacctctattggcaaaatctctcgtatcggcgaacaac


 


tggttctgtctggcgactacgcatccatcggccgcctgatgaacgtcaa


 


ccagggtctcctggacgccctgggcgttaacatcttagaactgagccag


 


ctgatctattccgctcgtgcggcaggtgcgtttggcgctaaaatcacgg


 


gcgctggcggcggtggctgtatggttgcgctgaccgctccggaaaaatg


 


caaccaagtggcagaagcggtagcaggcgctggcggtaaagtgactatc


 


actaaaccgaccgagcaaggtctgaaagtagattaaagtctagttaaag


 


tttaaacggtctccagcttggctgttttggcggatgagagaagattttc


 


agcctgatacagattaaatcagaacgcagaagcggtctgataaaacaga


 


atttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaa


 


ctcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccat


 


gcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcg


 


aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcc


 


tgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacg


 


gcccggagggtggcgggcaggacgcccgccataaactgccaggcatcaa


 


attaagcagaaggccatcctgacggatggcctttttgcgtttctacaaa


 


ctctttttgtttatttttctaaatacattcaaatatgtatccgctcatg


 


agacaataaccctgataaatgcttcaataatattgaaaaaggaagagta


 


tgagtattcaacatttccgtgtcgcccttattcccttttttgcggcatt


 


ttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagat


 


gctgaagatcagttgggtgcacgagtgggttacatcgaactggatctca


 


acagcggtaagatccttgagagttttcgccccgaagaacgttttccaat


 


gatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgtt


 


gacgccgggcaagagcaactcggtcgccgcatacactattctcagaatg


 


acttggttgagtactcaccagtcacagaaaagcatcttacggatggcat


 


gacagtaagagaattatgcagtgctgccataaccatgagtgataacact


 


gcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccg


 


cttttttgcacaacatgggggatcatgtaactcgccttgatcgttggga


 


accggagctgaatgaagccataccaaacgacgagcgtgacaccacgatg


 


cctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactac


 


ttactctagcttcccggcaacaattaatagactggatggaggcggataa


 


agttgcaggaccacttctgcgctcggcccttccggctggctggtttatt


 


gctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcag


 


cactggggccagatggtaagccctcccgtatcgtagttatctacacgac


 


ggggagtcaggcaactatggatgaacgaaatagacagatcgctgagata


 


ggtgcctcactgattaagcattggtaactgtcagaccaagtttactcat


 


atatactttagattgatttaaaacttcatttttaatttaaaaggatcta


 


ggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgag


 


ttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatctt


 


cttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaa


 


accaccgctaccagcggtggtttgtttgccggatcaagagctaccaact


 


ctttttccgaaggtaactggcttcagcagagcgcagataccaaatactg


 


tccttctagtgtagccgtagttaggccaccacttcaagaactctgtagc


 


accgcctacatacctcgctctgctaatcctgttaccagtggctgctgcc


 


agtggcgataagtcgtgtcttaccgggttggactcaagacgatagttac


 


cggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcc


 


cagcttggagcgaacgacctacaccgaactgagatacctacagcgtgag


 


ctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatc


 


cggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagg


 


gggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctga


 


cttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatgga


 


aaaacgccagcaacgcggcctttttacggttcctggccttttgctggcc


 


ttttgctcacatgttctttcctgcgttatcccctgattctgtggataac


 


cgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacga


 


ccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcg


 


gtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgc


 


actctcagtacaatctgctctgatgccgcatagttaagccagtatacac


 


tccgctatcgctacgtgactgggtcatggctgcgccccgacacccgcca


 


acacccgctgacgcgccctgacgggcttgtctgctcccggcatccgctt


 


acagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttc


 


accgtcatcaccgaaacgcgcgaggcagcagatcaattcgcgcgcgaag


 


gcgaagcggcatgcatttacgttgacaccatcgaatggtgcaaaacctt


 


tcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtg


 


aatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtct


 


cttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgc


 


gaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacatt


 


cccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattg


 


gcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgc


 


ggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcg


 


atggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatc


 


ttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatga


 


ccaggatgccattgctgtggaagctgcctgcactaatgttccggcgtta


 


tttcttgatgtctctgaccagacacccatcaacagtattattttctccc


 


atgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtca


 


ccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgt


 


ctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagc


 


cgatagcggaacgggaaggcgactggagtgccatgtccggttttcaaca


 


aaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggtt


 


gccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccg


 


ggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccga


 


agacagctcatgttatatcccgccgtcaaccaccatcaaacaggatttt


 


cgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg


 


gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaag


 


aaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttg


 


gccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg


 


ggcagtgagcgcaacgcaattaatgtgagttagcgcgaattgatctg


 


Amino acid sequence of MEA P. alba IspS


S288C G491S


MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNE


 


KAEFLTLLELIDNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLH


 


GTALSFRLLRQHGFEVSQEAFSGFKDQNGNFLENLKEDIKAILSLYEAS


 


FLALEGENILDEAKVFAISHLKELSEEKIGKELAEQVNHALELPLHRRT


 


QRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRETSRWWR


 


RVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFCFVTIID


 


DIYDVYGTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIA


 


YDNLKDKGENILPYLTKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAW


 


KSSSGPLQLVFAYFAVVQNIKKEEIENLQKYHDTISRPSHIFRLCNDLA


 


SASAEIARGETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKEKL


 


SGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPI


 


LPFER


 


DNA sequence of pDW161 harboring MEA P. alba


IspS S288C G491S


gtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtc


 


aggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactg


 


cataattcgtgtcgctcaaggcgcactcccgttctggataatgtttttt


 


gcgccgacatcataacggttctggcaaatattctgaaatgagctgttga


 


caattaatcatccggctcgtataatgtgtggaattgtgagcggataaca


 


atttcacacaggaaacagcgccgctgagaaaaagcgaagcggcactgct


 


ctttaacaatttatcagacaatctgtgtgggcactcgaccggaattatc


 


gattaactttattattaaaaattaaagaggtatatattaatgtatcgat


 


taaataaggaggaataaaccatggaagctcgtcgttctgcgaactacga


 


acctaacagctgggactatgattacctgctgtcctccgacacggacgag


 


tccatcgaagtatacaaagacaaagcgaaaaagctggaagccgaagttc


 


gtcgcgagattaataacgaaaaagcagaatttctgaccctgctggaact


 


gattgacaacgtccagcgcctgggcctgggttaccgtttcgagtctgat


 


atccgtggtgcgctggatcgcttcgtttcctccggcggcttcgatgcgg


 


taaccaagacttccctgcacggtacggcactgtctttccgtctgctgcg


 


tcaacacggttttgaggtttctcaggaagcgttcagcggcttcaaagac


 


caaaacggcaacttcctggagaacctgaaggaagatatcaaagctatcc


 


tgagcctgtacgaggccagcttcctggctctggaaggcgaaaacatcct


 


ggacgaggcgaaggttttcgcaatctctcatctgaaagaactgtctgaa


 


gaaaagatcggtaaagagctggcagaacaggtgaaccatgcactggaac


 


tgccactgcatcgccgtactcagcgtctggaagcagtatggtctatcga


 


ggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagctggca


 


attctggattacaacatgatccagtctgtataccagcgtgatctgcgtg


 


aaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaactgcactt


 


tgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagca


 


ttcgaaccgcaatactccgactgccgtaactccgtcgcaaaaatgtttt


 


gtttcgtaaccattatcgacgatatctacgatgtatacggcaccctgga


 


cgaactggagctgtttactgatgcagttgagcgttgggacgtaaacgcc


 


atcaacgacctgccggattacatgaaactgtgctttctggctctgtata


 


acactattaacgaaatcgcctacgacaacctgaaagataaaggtgagaa


 


catcctgccgtatctgaccaaagcctgggctgacctgtgcaacgctttc


 


ctgcaagaagccaagtggctgtacaacaaatctactccgacctttgacg


 


actacttcggcaacgcatggaaatcctcttctggcccgctgcaactggt


 


gttcgcttacttcgctgtcgtgcagaacattaaaaaggaagagatcgaa


 


aacctgcaaaaataccatgacaccatctctcgtccttcccatatcttcc


 


gtctgtgcaatgacctggctagcgcgtctgcggaaattgcgcgtggtga


 


aaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaa


 


gaactggctaccgaaagcgtgatgaatctgatcgatgaaacctggaaaa


 


agatgaacaaggaaaaactgagtggtagcctgttcgcgaaaccgttcgt


 


ggaaaccgcgatcaacctggcacgtcaatctcactgcacttatcataac


 


ggcgacgcgcatacctctccggatgagctgacccgcaaacgcgttctgt


 


ctgtaatcactgaaccgattctgccgtttgaacgctaactgcataaagg


 


aggtaaaaaaacatggtatcctgttctgcgccgggtaagatttacctgt


 


tcggtgaacacgccgtagtttatggcgaaactgcaattgcgtgtgcggt


 


ggaactgcgtacccgtgttcgcgcggaactcaatgactctatcactatt


 


cagagccagatcggccgcaccggtctggatttcgaaaagcacccttatg


 


tgtctgcggtaattgagaaaatgcgcaaatctattcctattaacggtgt


 


tttcttgaccgtcgattccgacatcccggtgggctccggtctgggtagc


 


agcgcagccgttactatcgcgtctattggtgcgctgaacgagctgttcg


 


gctttggcctcagcctgcaagaaatcgctaaactgggccacgaaatcga


 


aattaaagtacagggtgccgcgtccccaaccgatacgtatgtttctacc


 


ttcggcggcgtggttaccatcccggaacgtcgcaaactgaaaactccgg


 


actgcggcattgtgattggcgataccggcgttttctcctccaccaaaga


 


gttagtagctaacgtacgtcagctgcgcgaaagctacccggatttgatc


 


gaaccgctgatgacctctattggcaaaatctctcgtatcggcgaacaac


 


tggttctgtctggcgactacgcatccatcggccgcctgatgaacgtcaa


 


ccagggtctcctggacgccctgggcgttaacatcttagaactgagccag


 


ctgatctattccgctcgtgcggcaggtgcgtttggcgctaaaatcacgg


 


gcgctggcggcggtggctgtatggttgcgctgaccgctccggaaaaatg


 


caaccaagtggcagaagcggtagcaggcgctggcggtaaagtgactatc


 


actaaaccgaccgagcaaggtctgaaagtagattaaagtctagttaaag


 


tttaaacggtctccagcttggctgttttggcggatgagagaagattttc


 


agcctgatacagattaaatcagaacgcagaagcggtctgataaaacaga


 


atttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaa


 


ctcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccat


 


gcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcg


 


aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcc


 


tgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacg


 


gcccggagggtggcgggcaggacgcccgccataaactgccaggcatcaa


 


attaagcagaaggccatcctgacggatggcctttttgcgtttctacaaa


 


ctctttttgtttatttttctaaatacattcaaatatgtatccgctcatg


 


agacaataaccctgataaatgcttcaataatattgaaaaaggaagagta


 


tgagtattcaacatttccgtgtcgcccttattcccttttttgcggcatt


 


ttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagat


 


gctgaagatcagttgggtgcacgagtgggttacatcgaactggatctca


 


acagcggtaagatccttgagagttttcgccccgaagaacgttttccaat


 


gatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgtt


 


gacgccgggcaagagcaactcggtcgccgcatacactattctcagaatg


 


acttggttgagtactcaccagtcacagaaaagcatcttacggatggcat


 


gacagtaagagaattatgcagtgctgccataaccatgagtgataacact


 


gcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccg


 


cttttttgcacaacatgggggatcatgtaactcgccttgatcgttggga


 


accggagctgaatgaagccataccaaacgacgagcgtgacaccacgatg


 


cctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactac


 


ttactctagcttcccggcaacaattaatagactggatggaggcggataa


 


agttgcaggaccacttctgcgctcggcccttccggctggctggtttatt


 


gctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcag


 


cactggggccagatggtaagccctcccgtatcgtagttatctacacgac


 


ggggagtcaggcaactatggatgaacgaaatagacagatcgctgagata


 


ggtgcctcactgattaagcattggtaactgtcagaccaagtttactcat


 


atatactttagattgatttaaaacttcatttttaatttaaaaggatcta


 


ggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgag


 


ttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatctt


 


cttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaa


 


accaccgctaccagcggtggtttgtttgccggatcaagagctaccaact


 


ctttttccgaaggtaactggcttcagcagagcgcagataccaaatactg


 


tccttctagtgtagccgtagttaggccaccacttcaagaactctgtagc


 


accgcctacatacctcgctctgctaatcctgttaccagtggctgctgcc


 


agtggcgataagtcgtgtcttaccgggttggactcaagacgatagttac


 


cggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcc


 


cagcttggagcgaacgacctacaccgaactgagatacctacagcgtgag


 


ctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatc


 


cggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagg


 


gggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctga


 


cttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatgga


 


aaaacgccagcaacgcggcctttttacggttcctggccttttgctggcc


 


ttttgctcacatgttctttcctgcgttatcccctgattctgtggataac


 


cgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacga


 


ccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcg


 


gtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgc


 


actctcagtacaatctgctctgatgccgcatagttaagccagtatacac


 


tccgctatcgctacgtgactgggtcatggctgcgccccgacacccgcca


 


acacccgctgacgcgccctgacgggcttgtctgctcccggcatccgctt


 


acagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttc


 


accgtcatcaccgaaacgcgcgaggcagcagatcaattcgcgcgcgaag


 


gcgaagcggcatgcatttacgttgacaccatcgaatggtgcaaaacctt


 


tcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtg


 


aatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtct


 


cttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgc


 


gaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacatt


 


cccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattg


 


gcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgc


 


ggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcg


 


atggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatc


 


ttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatga


 


ccaggatgccattgctgtggaagctgcctgcactaatgttccggcgtta


 


tttcttgatgtctctgaccagacacccatcaacagtattattttctccc


 


atgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtca


 


ccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgt


 


ctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagc


 


cgatagcggaacgggaaggcgactggagtgccatgtccggttttcaaca


 


aaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggtt


 


gccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccg


 


ggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccga


 


agacagctcatgttatatcccgccgtcaaccaccatcaaacaggatttt


 


cgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg


 


gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaag


 


aaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttg


 


gccgattcattaatgcagctggcacgacaggtttcccgactggaaagcg


 


ggcagtgagcgcaacgcaattaatgtgagttagcgcgaattgatctg






Example 3
The S288C Mutation Increases the Solubility of IspS Enzymes from Salix alba and Salix babylonica 
The effect of S288C mutation on solubility of IspS from Salix alba and Salix babylonica was investigated.
Methods and Results
Construction of Plasmid pEWL792 (pTrc S. alba IspS)
Generation of a synthetic gene encoding Salix alba isoprene synthase (S. alba IspS) was performed by Gene Oracle Inc. (Mountain View, Calif.) utilizing a codon optimization method specific for E. coli expression. The synthetic gene was cloned into plasmid pGOv4 with an engineered NcoI restriction site on the 5′-end and a PstI restriction site on the 3′-end. Clustal W sequence alignment between P. alba IspS and S. alba IspS shows 91.73% identity (FIG. 7).
1 μg of the pGOv4 S. alba IspS plasmid was digested with restriction endonucleases NcoI and PstI (Roche) based on the manufacturer's protocol at 37° C. The digested S. alba IspS fragment was then purified using a 1% EX gel (Invitrogen) and extracted using the QIAquick Gel Extraction Kit (Qiagen). 0.5 μg of pTrcHis2B plasmid (Invitrogen) was digested with restriction endonucleases NcoI and PstI based on the manufacturer's protocol at 37° C. The pTrcHis2B plasmid was then purified using a 1% EX gel and extracted using the QIAquick Gel Extraction Kit. The S. alba IspS gene was ligated into the pTrcHis2B plasmid using T4 DNA ligase (New England Biolabs) based on the manufacturer's protocol at room temperature. The ligation mixture was desalted by floating a 0.025 μm nitrocellulose membrane filter (Millipore) in a petri dish of ddH2O and applying the ligation mixture gently on top of the nitrocellulose membrane filter for 30 minutes at room temperature. Strain MCM446 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 5 μl of desalted pTrc S. alba IspS ligation mix. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator (BioRad). Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+10 mM mevalonic acid plates and incubated overnight at 37° C. The next day, several transformants were picked and grown in 5 ml LB+50 μg/μl carbenicillin tubes overnight at 30° C. Plasmid preps were performed on the overnight cultures using QIAquick Spin Miniprep Kit (Qiagen). Plasmids were digested with restriction endonucleases NcoI and PstI at 37° C. and analyzed on a 1.2% E-gel (Invitrogen) to ensure correct sized fragments were obtained. Plasmids from several transformants were shipped to Sequetech (Mountain View, Calif.) for sequencing with primers EL1005, EL1006, EL1270, and EL1271 (Table 7). DNA sequencing results showed that plasmids were correct. One plasmid was selected and designated as pEWL792 (FIG. 8, Table 8).
Construction of Plasmid pEWL795 (pTrc S. alba IspS-mMVK)
A PCR reaction was performed to amplify the Methanosarcina mazei MVK gene using plasmid MCM376 as the template, primers MCM165 and MCM177, and Pfu Ultra II Fusion DNA polymerase (Agilent) according to manufacturer's protocol. PCR conditions were as follows: 95° C. for 2 minutes (first cycle only), 95° C. for 25 seconds, 55° C. for 25 seconds, 72° C. for 18 seconds, repeat for 29 cycles, with final extension at 72° C. for 2 minutes. The M. mazei MVK PCR product was purified using QIAquick PCR Purification Kit (Qiagen).
The M. mazei MVK PCR product was digested with restriction endonuclease PmeI (New England Biolabs) based on manufacturer's protocol at 37° C. The digested PCR fragment was then purified using the QIAquick PCR Purification Kit. A sequential restriction digest was performed with restriction endonuclease NsiI (Roche) based on manufacturer's protocol at 37° C. The digested M. mazei MVK fragment was then purified using the QIAquick PCR Purification Kit. Plasmid pEWL792 was digested with restriction endonuclease PmeI based on manufacturer's protocol at 37° C. The digested pEWL792 fragment was then purified using the QIAquick PCR Purification Kit. A sequential restriction digest was performed with restriction endonuclease NsiI based on manufacturer's protocol at 37° C. The digested pEWL792 fragment was then gel purified using a 1% EX gel and extracted using the QIAquick Gel Extraction Kit. The M. mazei MVK gene was ligated into the pEWL792 plasmid using T4 DNA ligase based on the manufacturer's protocol overnight at 16° C. The next day, the ligation mixture was desalted by floating a 0.025 μm nitrocellulose membrane filter in a petri dish of ddH2O and applying the ligation mixture gently on top of the nitrocellulose membrane filter for 30 minutes at room temperature. MCM446 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50111 of cell suspension with 5 μl of desalted pTrc S. alba IspS-mMVK ligation mix. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+10 mM mevalonic acid plates and incubated overnight at 37° C. The next day, several transformants were picked and grown in 5 ml LB+50 μg/μl carbenicillin tubes overnight at 30° C. Plasmid preps were performed on the overnight cultures using QIAquick Spin Miniprep Kit (Qiagen). Plasmids were digested with restriction endonucleases NcoI (Roche) and PmeI (New England Biolabs) using Buffer 4 (New England Biolabs) at 37° C. and analyzed on a 1.2% E-gel to ensure correct sized fragments were obtained. Plasmids from several transformants were shipped to Sequetech for sequencing with primers EL1003, EL1005, EL1006, EL1270, EL1271, and EL1272 (Table 7). DNA sequencing results showed that plasmids were correct. One plasmid was selected and designated as pEWL795 (FIG. 9, Table 8).
Construction of Strain EWL804 (BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA, pTrc S. alba IspS-mMVK)
Strain CMP451 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 11 μl of plasmid pEWL795. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+5 mM mevalonic acid plates and incubated overnight at 37° C. One colony was selected and designated as strain EWL804 (Table 9).
Construction of Strain EWL810 (BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA, pTrc S. alba IspS-mMVK, pCL Ptrc-Upper MVA)
Strain EWL804 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 11 μl of plasmid MCM82 (pCL Ptrc Upper MVA). The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+50 μg/μl spectinomycin plates and incubated overnight at 37° C. One colony was selected and designated as strain EWL810 (Table 9)
Construction of Plasmid pEWL834 (pTrc S. babylonica IspS)
Generation of a synthetic gene encoding Salix babylonica isoprene synthase (S. babylonica IspS) was performed by Gene Oracle Inc. (Mountain View, Calif.) utilizing a codon optimization method specific for E. coli expression. The synthetic gene was cloned into plasmid pGOv4 with an engineered NcoI restriction site on the 5′-end and a PstI restriction site on the 3′-end. Clustal W sequence alignment between P. alba IspS and S. babylonica IspS shows 92.28% identity (FIG. 10).
1 μg of the pGOv4 S. babylonica IspS plasmid was digested with restriction endonucleases NcoI and PstI based on the manufacturer's protocol at 37° C. The digested S. babylonica IspS fragment was then purified using a 1% EX gel and extracted using the QIAquick Gel Extraction Kit. Digested 0.5 μg of pTrcHis2B plasmid with restriction endonucleases NcoI and PstI based on the manufacturer's protocol at 37° C. The pTrcHis2B plasmid was then purified using a 1% EX gel and extracted using the QIAquick Gel Extraction Kit. The S. babylonica IspS gene was ligated into the pTrcHis2B plasmid using T4 DNA ligase based on the manufacturer's protocol at room temperature. The ligation mixture was desalted by floating a 0.025 μm nitrocellulose membrane filter (Millipore) in a petri dish of ddH2O and applying the ligation mixture gently on top of the nitrocellulose membrane filter for 30 minutes at room temperature. Strain MCM446 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 5 μl of desalted pTrc S. baylonica IspS ligation mix. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+10 mM mevalonic acid plates and incubated overnight at 37° C. The next day, several transformants were picked and grown in 5 ml LB+50 μg/μl carbenicillin tubes overnight at 30° C. Plasmid preps were performed on the overnight cultures using QIAquick Spin Miniprep Kit. Plasmids were digested with restriction endonucleases NcoI and PstI at 37° C. and analyzed on a 1.2% E-gel to ensure correct sized fragments were obtained. Plasmids from several transformants were shipped to Sequetech for sequencing with primers EL1004, EL1006, EL1285, EL1286, EL1287 (Table 7). DNA sequencing results showed that plasmids were correct. One plasmid was selected and designated as pEWL834 (FIG. 11, Table 8).
Construction of Plasmid pEWL851 (pTrc S. babylonica IspS-mMVK)
A PCR reaction was performed to amplify the Methanosarcina mazei MVK gene using plasmid MCM376 as the template, primers MCM165 and MCM177, and Pfu Ultra II Fusion DNA polymerase (Agilent) according to manufacturer's protocol. PCR conditions were as follows: 95° C. for 2 minutes (first cycle only), 95° C. for 25 seconds, 55° C. for 25 seconds, 72° C. for 18 seconds, repeat for 29 cycles, with final extension at 72° C. for 2 minutes. The M. mazei MVK PCR product was purified using QIAquick PCR Purification Kit.
The M. mazei MVK PCR product was digested with restriction endonuclease PmeI (New England Biolabs) based on manufacturer's protocol at 37° C. The digested PCR fragment was then purified using the QIAquick PCR Purification Kit. A sequential restriction digest was performed with restriction endonuclease NsiI based on manufacturer's protocol at 37° C. The digested M. mazei MVK fragment was then purified using the QIAquick PCR Purification Kit. Plasmid pEWL834 was digested with restriction endonuclease PmeI based on manufacturer's protocol at 37° C. The digested pEWL834 fragment was then purified using the QIAquick PCR Purification Kit. A sequential restriction digest was performed with restriction endonuclease NsiI based on manufacturer's protocol at 37° C. The digested pEWL834 fragment was then gel purified using a 1% EX gel and extracted using the QIAquick Gel Extraction Kit. The M. mazei MVK gene was ligated into the pEWL834 plasmid using T4 DNA ligase based on the manufacturer's protocol overnight at 16° C. The next day, the ligation mixture was desalted by floating a 0.025 μm nitrocellulose membrane filter in a petri dish of ddH2O and applying the ligation mixture gently on top of the nitrocellulose membrane filter for 30 minutes at room temperature. MCM446 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 5 μl of desalted pTrc S. babylonica IspS-mMVK ligation mix. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+10 mM mevalonic acid plates and incubated overnight at 37° C. The next day, several transformants were picked and grown in 5 ml LB+50 μg/μl carbenicillin tubes overnight at 30° C. Plasmid preps were performed on the overnight cultures using QIAquick Spin Miniprep Kit. Plasmids were digested with restriction endonucleases NcoI and PmeI using Buffer 4 (New England Biolabs) at 37° C. and analyzed on a 1.2% E-gel to ensure correct sized fragments were obtained. Plasmids from several transformants were shipped to Sequetech for sequencing with primers EL1004, EL1005, EL1006, EL1285, EL1286, and EL1287 (Table 6). DNA sequencing confirmed the plasmid sequences. One plasmid was selected and designated as pEWL851 (FIG. 12, Table 8).
Construction of Strain EWL887 (BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA, pTrc S. babylonica IspS-mMVK)
Strain CMP451 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 11 μl of plasmid pEWL851. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+5 mM mevalonic acid plates and incubated overnight at 37° C. One colony was selected and designated as strain EWL887 (Table 9).
Construction of Strain EWL893 (BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA, pTrc S. babylonica IspS-mMVK, MCM82)
Strain EWL887 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 11 μl of plasmid MCM82. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+50 μg/μl spectinomycin plates and incubated overnight at 37° C. One colony was selected and designated as strain EWL893 (Table 9).
Construction of Strain EWL900 (BL21, PL.2-mKKDyI, pTrc S. alba IspS) and EWL903 (BL21, PL.2-mKKDyI, pTrc S. babylonica IspS)
Strain MCM531 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 11 μl of plasmid pEWL792 (pTrc S. alba IspS) or pEWL834 (pTrc S. babylonica IspS). The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+5 mM mevalonic acid plates and incubated overnight at 37° C. For transformants harboring the plasmid pEWL792, one colony was selected and designated as strain EWL900 (Table 9). For transformants harboring the plasmid pEWL834, one colony was selected and designated as strain EWL903 (Table 9).
Comparing Solubility of P. alba IspS, S. alba IspS, and S. babylonica IspS
Strains expressing pTrc P. alba IspS (DW194), pTrc S. alba IspS (EWL900) and pTrc S. babylonica IspS (EWL903) were grown in LB media, induced at OD600 ̃0.5 with 200 μM IPTG, and induced for 4 hours. Cell pellets were collected by centrifugation and stored at −80° C. The cells were lysed by French Press and analyzed for protein solubility by Western Blot. Results showed that S. alba IspS and S. babylonica IspS are more insoluble than P. alba IspS (FIG. 13 and FIG. 14).
Construction of pEWL906 (pTrc S. alba IspS S288C) and pEWL907 (pTrc S. babylonica IspS S288C)
Primers were designed to introduce the S288C mutation into S. alba IspS (primers EL1288 and EL1289, see Table 7) and S. babylonica IspS (primers EL1290 and EL1291, see Table 7). Plasmid pEWL792 (pTrc S. alba IspS) and pEWL834 (pTrc S. babylonica IspS) were used as the starting templates for PCR reactions with the Pfu Ultra II Fusion DNA Polymerase. PCR conditions were as follows: 95° C. for 2 minutes (first cycle only), 95° C. for 30 seconds, 55° C. for 1 minute, 68° C. for 6 minute, repeat for 16 cycles. The parental template for each PCR reaction were digested by addition of 21 μl DpnI restriction endonuclease (Roche) and incubated at 37° C. for 2 hours. Strain MCM531 cells were grown in LB to midlog phase and then washed three times in ice-cold, sterile water. Mixed 50 μl of cell suspension with 21 μl of each PCR reaction. The cell suspension mixture was electroporated in a 2 mm cuvette at 2.5 Volts and 25 μFd using a Gene Pulser Xcell Electroporator. Cells were recovered in 1 ml of LB and incubated for 2 hours at 30° C. with shaking. Transformants were selected on LA+50 μg/μl carbenicillin+5 mM mevalonic acid plates and incubated overnight at 37° C. The next day, several transformants were picked from each and grown in 5 ml LB+50 μg/μl carbenicillin tubes overnight at 30° C. Plasmid preps were performed on the overnight cultures using QIAquick Spin Miniprep Kit. Plasmids from several transformants were shipped to Sequetech for sequencing. Primers EL1005, EL1006, EL1270, EL1271, and EL1272 were used for sequencing pTrc S. alba IspS S288C (see Table 7). Primers EL1005, EL1006, EL1285, EL1286, and EL1287 were used for sequencing pTrc S. babylonica IspS S288C (see Table 7).
DNA sequencing results showed that plasmids were correct and contained the S288C mutation. Plasmid pTrc S. alba IspS S288C was designated pEWL906 (FIG. 15, Table 8). Plasmid pTrc S. babylonica IspS S288C was designated pEWL907 (FIG. 16, Table 8). Plasmid pEWL906 was transformed into MCM531 cells to generate the expression strain EWL913 (Table 9). Plasmid pEWL907 was transformed into MCM531 cells to generate expression strain EWL916 (Table 9).
Comparing Solubility of P. alba IspS, S. alba IspS, S. alba IspS S288C, S. babylonica IspS, and S. babylonica IspS S288C
Strains expressing P. alba IspS (DW194), S. alba IspS (EWL900), S. alba IspS S288C (EWL913), S. babylonica IspS (EWL903), or S. babylonica IspS S288C (EWL916) were grown in LB+50 μg/μl carbenicillin. Cells were induced with 200 μM IPTG when OD600 ̃0.5 and grown for 4 hours at 34° C. Cell pellets were collected by centrifugation and stored at −80° C. The cells were lysed by French Press and analyzed for protein solubility on 10% NuPage gel (Invitrogen) by Coomassie staining (FIG. 17) and Western blot (FIG. 18). Results showed that the S288C mutation improved solubility of both S. alba IspS and S. babylonica IspS.







TABLE 7 







Primer sequences








Primer name
Primer sequence





EL1003
GATAGTAACGGCTGCGCTGCTACC


 


EL1004
ACAATTTCACACAGGAAACAGC


 


EL1005
CCAGGCAAATTCTGTTTTATCAG


 


EL1006
GACAGCTTATCATCGACTGCACG


 


EL1270
CCTCCTTTCACTTCACGAAGC


 


EL1271
GCTTTTCACTGACGCAGTTGAG


 


EL1272
GATAAACAATGAGACCGCAG


 


EL1285
GGAGAACTTGAAAGAGGAC


 


EL1286
GTACAACACAATCAATGAGATAG


 


EL1287
GATGCACATACATCACCTGATG


 


EL1288
GCAGAATTTCTGTCGCTAAGATGTTCTGTTTCGT



AACCATTATCGACGAC


 


EL1289
GTCGTCGATAATGGTTACGAAACAGAACATCTTA



GCGACAGAAATTCTGC


 


EL1290
CGCAAATCTGTGGCTAAAATGTTTTGTTTCGTGA



CCATAATTGATGAC


 


EL1291
GTCATCAATTATGGTCACGAAACAAAACATTTTA



GCCACAGATTTGCG


 


MCM165
GCGAACGATGCATAAAGGAGGTAAAAAAACATGG



TATCCTGTTCTGCGCCGGGTAAGATTTACCTG


 


MCM177
GGGCCCGTTTAAACTTTAACTAGACTTTAATCTA



CTTTCAGACCTTGC
















TABLE 8







Plasmids used in this study









Plasmid name
Antibiotic resistance
Description





pEWL792
Carbenicillin
pTrc S. alba IspS


pEWL795
Carbenicillin
pTrc S. alba IspS-mMVK


pEWL834
Carbenicillin
pTrc S. babylonic IspS


pEWL851
Carbenicillin
pTrc S. babylonic IspS-mMVK


pEWL906
Carbenicillin
pTrc S. alba IspS S288C


pEWL907
Carbenicillin
pTrc S. babylonica IspS S288C
















TABLE 9







Strains used in this study










Antibiotic



Strain name
resistance
Description





EWL804
Carbenicillin
BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA,




pTrc S. alba IspS-mMVK


EWL810
Carbenicillin,
BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA,



Spectinomycin
pTrc S. alba IspS-mMVK, pCL Ptrc-




Upper MVA


EWL887
Carbenicillin
BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA,




pTrc S. babylonica IspS-mMVK


EWL893
Carbenicillin,
BL21, pgl+ PL.2-mKKDyI, GI1.2-gltA,



Spectinomycin
pTrc S. babylonica IspS-mMVK, pCL




Ptrc-Upper MVA


EWL900
Carbenicillin
BL21, PL.2-mKKDyI, pTrc S. alba IspS


EWL903
Carbenicillin
BL21, PL.2-mKKDyI, pTrc S. babylonica




IspS


EWL913
Carbenicillin
BL21, PL.2-mKKDyI, pTrc S. alba IspS




S288C


EWL916
Carbenicillin
BL21, PL.2-mKKDyI, pTrc S. babylonica




IspS S288C

















DNA Sequence of pEWL792



GTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCAT


 


CGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAG


 


GCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATA


 


TTCTGAAATGAGCTGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAG


 


CGGATAACAATTTCACACAGGAAACAGCGCCGCTGAGAAAAAGCGAAGCGGCACTGC


 


TCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGACCGGAATTATCGATTAACTT


 


TATTATTAAAAATTAAAGAGGTATATATTAATGTATCGATTAAATAAGGAGGAATAAA


 


CCATGGAAACCAGACGGTCCGCTAATTATGAACCGAACAGTTGGGATTACGATTACCT


 


GCTTTCTTCGGATACGGAAGATGCCATAGAGGTATACAAAGACAAAGCGAAGAAACTG


 


GATGCTGAAGTACGCTCGAAGATAAACAATGAGACCGCAGAATTTTTAACGCAATTAG


 


AACTGATTGACACTATCCAGCGCCTGGGTTTGGGTTATCGTTTCGAAAGCGACATTAGA


 


CGCGCCTTGGATCGTTTCGTGAGTAGTGGTGGGTTCGAGGCTGTCGCGAAAACTTCACT


 


GCAAGCTACAGCACTGTCTTTTCGGCTGCTGCGTCAACATGGTTTCGAGGTTAGTCAAG


 


AAGTGTTTAATGGTTTCAAAGACCAGAACGGTAACTTTATGGAGGATTTAAAAGAAGA


 


TATTAAGGCCCTCCTTTCACTTCACGAAGCATCTTTTTTAGCTCTGGAAGGCGAGAATA


 


TTCTGGACGAGGCAAAGGTATTCACAATTAGCCACCTCAAAGAACTGAACGAGGAGAA


 


AATTGGCAAAGACATGGTGGAACAAGTGAACCATGCTCTGGAATTACCCCTTCATCGT


 


CGTACGCAGCGTTTGGAGGCTGTGTGGTCTATTGAAGCGTATCGGAAGAAGGAAGATG


 


CAAACCGCGTCCTTCTGGAACTTGCAATCCTCGATTATAATATGGTCCAATCTGTGTAT


 


CAGCGCGATTTGCGCGAAACTTCGAGATGGTGGCGCCGCGTGGGATTAGCCACTAAGT


 


TGCATTTCGCCAGAGACAGACTTATCGAATCCTTCTATTGGGCGGTTGGCGTCGCCTTT


 


GAACCGCAATACTCAGATTGCAGAATTTCTGTCGCTAAGATGTTCTCTTTCGTAACCAT


 


TATCGACGACATTTATGATGTGTATGGGACGTTGGAAGAGCTGGAGCTTTTCACTGACG


 


CAGTTGAGAGATGGGATGTGAGCGCCATTGACGATTTACCCGATTATATGAAACTGTG


 


CTTCTTGGCGTTGTACAATACTATAAACGAAATCGCTTATGACAATCTTAAAGAAAAAG


 


GCGAAAATATTCTGCCGTATCTGACTAAAGCCTGGGCGGATCTTTGCAATGCTTTCCTG


 


CAAGAGGCACGTTTTTTATATAACAAGTCGACACCTACCTTCTCAGATTATTTTGGCAA


 


TGCGTGGAAATCTAGTTCTGGGCCACTTCAACTGGTTTTTGCTTACTTTGCCGTAGTGCA


 


AAACATCAAAAAGGAAGAAACCGAAAATCTGCTGAAATATCATGATATAATCTCTTGG


 


CCATCGTACATATTTCGGCTGTGTAATGATCTGGCTTCCGCATCGGCAGAAATTGCGCG


 


GGGTGAGACTGCAAACAGCGTTTCTTGTTACATGCGGACGAAGGGAATTTCCGAAGAA


 


CTGGCAACCGAATCAGTGATGAATCTTATTGATGAAACGTGGAAAAAGATGAACAAGG


 


AAAAATTGGGGGACTCACTCTTTGCAAAACACTTTGTGGAGACCGCGATAAACCTTGCT


 


CGCCAATCGCATTGTACGTATCATAATGGAGATGCGCATACTTCGCCTGACGAATTAAC


 


CCGCAAACGCGTACTGTCAGTGATCACTGAACCGATACTGCCATTGGAACGGTGACTG


 


CAGCTGGTACCATATGGGAATTCGAAGCTTTCTAGAACAAAAACTCATCTCAGAAGAG


 


GATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAG


 


CTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACG


 


CAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCT


 


GACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTC


 


CCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAA


 


GACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT


 


CCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGA


 


CGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCC


 


TTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGC


 


TCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAG


 


TATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT


 


GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAG


 


TGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA


 


GAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG


 


TGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG


 


GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT


 


TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACG


 


ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTC


 


GCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACAC


 


CACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT


 


ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGAC


 


CACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT


 


GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTA


 


TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAT


 


CGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCAT


 


ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATC


 


CTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCA


 


GACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTG


 


CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAG


 


CTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGT


 


CCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAT


 


ACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTT


 


ACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGG


 


GGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCT


 


ACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTA


 


TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAA


 


CGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT


 


GTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTA


 


CGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATT


 


CTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACG


 


ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTC


 


TCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCT


 


CTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGG


 


CTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCG


 


GCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTC


 


ACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCG


 


GCATGCATTTACGTTGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATA


 


GCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGAT


 


GTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAG


 


CCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTAC


 


ATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTG


 


CCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGC


 


GCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAG


 


CCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAAC


 


TATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGC


 


GTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGA


 


CGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTG


 


TTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATA


 


TCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATG


 


TCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCT


 


GGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTG


 


CGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTT


 


ATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGT


 


GGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCC


 


GTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCC


 


GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGG


 


GCAGTGAGCGCAACGCAATTAATGTGAGTTAGCGCGAATTGATCTG


 


DNA sequence of pEWL795


GTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCAT


 


CGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAG


 


GCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATA


 


TTCTGAAATGAGCTGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAG


 


CGGATAACAATTTCACACAGGAAACAGCGCCGCTGAGAAAAAGCGAAGCGGCACTGC


 


TCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGACCGGAATTATCGATTAACTT


 


TATTATTAAAAATTAAAGAGGTATATATTAATGTATCGATTAAATAAGGAGGAATAAA


 


CCATGGAAACCAGACGGTCCGCTAATTATGAACCGAACAGTTGGGATTACGATTACCT


 


GCTTTCTTCGGATACGGAAGATGCCATAGAGGTATACAAAGACAAAGCGAAGAAACTG


 


GATGCTGAAGTACGCTCGAAGATAAACAATGAGACCGCAGAATTTTTAACGCAATTAG


 


AACTGATTGACACTATCCAGCGCCTGGGTTTGGGTTATCGTTTCGAAAGCGACATTAGA


 


CGCGCCTTGGATCGTTTCGTGAGTAGTGGTGGGTTCGAGGCTGTCGCGAAAACTTCACT


 


GCAAGCTACAGCACTGTCTTTTCGGCTGCTGCGTCAACATGGTTTCGAGGTTAGTCAAG


 


AAGTGTTTAATGGTTTCAAAGACCAGAACGGTAACTTTATGGAGGATTTAAAAGAAGA


 


TATTAAGGCCCTCCTTTCACTTCACGAAGCATCTTTTTTAGCTCTGGAAGGCGAGAATA


 


TTCTGGACGAGGCAAAGGTATTCACAATTAGCCACCTCAAAGAACTGAACGAGGAGAA


 


AATTGGCAAAGACATGGTGGAACAAGTGAACCATGCTCTGGAATTACCCCTTCATCGT


 


CGTACGCAGCGTTTGGAGGCTGTGTGGTCTATTGAAGCGTATCGGAAGAAGGAAGATG


 


CAAACCGCGTCCTTCTGGAACTTGCAATCCTCGATTATAATATGGTCCAATCTGTGTAT


 


CAGCGCGATTTGCGCGAAACTTCGAGATGGTGGCGCCGCGTGGGATTAGCCACTAAGT


 


TGCATTTCGCCAGAGACAGACTTATCGAATCCTTCTATTGGGCGGTTGGCGTCGCCTTT


 


GAACCGCAATACTCAGATTGCAGAATTTCTGTCGCTAAGATGTTCTCTTTCGTAACCAT


 


TATCGACGACATTTATGATGTGTATGGGACGTTGGAAGAGCTGGAGCTTTTCACTGACG


 


CAGTTGAGAGATGGGATGTGAGCGCCATTGACGATTTACCCGATTATATGAAACTGTG


 


CTTCTTGGCGTTGTACAATACTATAAACGAAATCGCTTATGACAATCTTAAAGAAAAAG


 


GCGAAAATATTCTGCCGTATCTGACTAAAGCCTGGGCGGATCTTTGCAATGCTTTCCTG


 


CAAGAGGCACGTTTTTTATATAACAAGTCGACACCTACCTTCTCAGATTATTTTGGCAA


 


TGCGTGGAAATCTAGTTCTGGGCCACTTCAACTGGTTTTTGCTTACTTTGCCGTAGTGCA


 


AAACATCAAAAAGGAAGAAACCGAAAATCTGCTGAAATATCATGATATAATCTCTTGG


 


CCATCGTACATATTTCGGCTGTGTAATGATCTGGCTTCCGCATCGGCAGAAATTGCGCG


 


GGGTGAGACTGCAAACAGCGTTTCTTGTTACATGCGGACGAAGGGAATTTCCGAAGAA


 


CTGGCAACCGAATCAGTGATGAATCTTATTGATGAAACGTGGAAAAAGATGAACAAGG


 


AAAAATTGGGGGACTCACTCTTTGCAAAACACTTTGTGGAGACCGCGATAAACCTTGCT


 


CGCCAATCGCATTGTACGTATCATAATGGAGATGCGCATACTTCGCCTGACGAATTAAC


 


CCGCAAACGCGTACTGTCAGTGATCACTGAACCGATACTGCCATTGGAACGGTGACTG


 


CATAAAGGAGGTAAAAAAACATGGTATCCTGTTCTGCGCCGGGTAAGATTTACCTGTTC


 


GGTGAACACGCCGTAGTTTATGGCGAAACTGCAATTGCGTGTGCGGTGGAACTGCGTA


 


CCCGTGTTCGCGCGGAACTCAATGACTCTATCACTATTCAGAGCCAGATCGGCCGCACC


 


GGTCTGGATTTCGAAAAGCACCCTTATGTGTCTGCGGTAATTGAGAAAATGCGCAAATC


 


TATTCCTATTAACGGTGTTTTCTTGACCGTCGATTCCGACATCCCGGTGGGCTCCGGTCT


 


GGGTAGCAGCGCAGCCGTTACTATCGCGTCTATTGGTGCGCTGAACGAGCTGTTCGGCT


 


TTGGCCTCAGCCTGCAAGAAATCGCTAAACTGGGCCACGAAATCGAAATTAAAGTACA


 


GGGTGCCGCGTCCCCAACCGATACGTATGTTTCTACCTTCGGCGGCGTGGTTACCATCC


 


CGGAACGTCGCAAACTGAAAACTCCGGACTGCGGCATTGTGATTGGCGATACCGGCGT


 


TTTCTCCTCCACCAAAGAGTTAGTAGCTAACGTACGTCAGCTGCGCGAAAGCTACCCGG


 


ATTTGATCGAACCGCTGATGACCTCTATTGGCAAAATCTCTCGTATCGGCGAACAACTG


 


GTTCTGTCTGGCGACTACGCATCCATCGGCCGCCTGATGAACGTCAACCAGGGTCTCCT


 


GGACGCCCTGGGCGTTAACATCTTAGAACTGAGCCAGCTGATCTATTCCGCTCGTGCGG


 


CAGGTGCGTTTGGCGCTAAAATCACGGGCGCTGGCGGCGGTGGCTGTATGGTTGCGCT


 


GACCGCTCCGGAAAAATGCAACCAAGTGGCAGAAGCGGTAGCAGGCGCTGGCGGTAA


 


AGTGACTATCACTAAACCGACCGAGCAAGGTCTGAAAGTAGATTAAAGTCTAGTTAAA


 


GTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATA


 


CAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTA


 


GCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGA


 


TGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACG


 


AAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTC


 


TCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGG


 


AGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGC


 


CATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATAC


 


ATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA


 


AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGC


 


ATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAG


 


ATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCT


 


TGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTAT


 


GTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA


 


CTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATG


 


GCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC


 


CAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC


 


ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC


 


CAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACT


 


ATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGG


 


CGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT


 


GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAG


 


ATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGA


 


TGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTG


 


TCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAA


 


AGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT


 


TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTT


 


TTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTT


 


TGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGC


 


GCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACT


 


CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGT


 


GGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGC


 


AGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTA


 


CACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGG


 


AGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGG


 


GAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTG


 


ACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCC


 


AGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTT


 


CCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATAC


 


CGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA


 


GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTG


 


CACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATC


 


GCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCC


 


TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAG


 


CTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAAT


 


TCGCGCGCGAAGGCGAAGCGGCATGCATTTACGTTGACACCATCGAATGGTGCAAAAC


 


CTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTG


 


AAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTC


 


CCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGC


 


GGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAA


 


CAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAAT


 


TGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATG


 


GTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAAC


 


GCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAA


 


GCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAA


 


CAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCAT


 


TGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTG


 


CGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAAC


 


GGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGA


 


GGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATG


 


CGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACG


 


ACGATACCGAAGACAGCTCATGTTATATCCCGCCGTCAACCACCATCAAACAGGATTTT


 


CGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGG


 


TGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCC


 


CAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGAC


 


AGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCGCG


 


AATTGATCTG


 


Sequence of codon optimized S. babylonica IspS with engineered


NcoI restriction site on the 5′-end and an and PstI sites


CCATGGAAACCCGCAGAACCGCCAATTATGAACCCAATTCCTGGGATTACGATTACCTT


 


TTGAGTAGCGATAACGATGACGCCATAGAACTGTACAAAGATAAAGCCAAGAAACTTG


 


ATGCAGAAGTGCGTAGTAAAATTAATAACGAAAAAGCAGAATTTCTTACACAACTTGA


 


ACTCATTGATACTATCCAACGTCTCGGCCTCGGATACCGCTTCGAATCAGATATACGGC


 


GCGCTATCGATAGATATGTTTCATCGGGCGGATTCGAGGCAGTGGCTAAAACGTCGTTA


 


CACGCAACAGCACTCAGTTTTCGTTTGTTACGCCAACATGGATTCGAAGTGAGCCAAGA


 


GGTATTTTCGGGTTTCAAGGACCAAAATGGAAACTTTATGGAGAACTTGAAAGAGGAC


 


ATAAAGGCAATTCTTAGTCTCTATGAAGCGAGTTTCCTTGCTTTAGAAGGCGAGAATAT


 


ATTGGACGAAGCCAAGGTCTTTACAATCTCCCACCTGAAAGAGCTGAATGAGGAAAAA


 


ATTGGAAAAGACCTGGCCGACCAAGTGAATCATGCGCTGGAACTTCCTCTGCATCGGC


 


GTACCCAGCGTCTTGAAGCGGTATGGTCGATAGAAGCCTATCGCAAAAAGGAAGGAGC


 


GAATCGCGCATTACTGGAACTGGCGATCTTGGACTACAATATGGTCCAATCAGTCTACC


 


AACGCGATTTGCGCGAAACCTCCCGCTGGTGGCGCCGTGTAGGCTTAGCAACTAAATT


 


GCATTTTGCACGTGACCGGCTGATTGAATCTTTCTATTGGGCGGTCGGCGTTGCCTTTG


 


AGCCGCAATATTCTGACTGTCGCAAATCTGTGGCTAAAATGTTTTCATTCGTGACCATA


 


ATTGATGACATATATGATGTTTATGGGACGTTAGATGAGTTAGAGTTGTTTACCGACGC


 


AGTTGAACGCTGGGACGTTTCTGCCGTGGATGACCTCCCGGATTATATGAAATTATGCT


 


TTCTGGCCCTGTACAACACAATCAATGAGATAGCATACGACAATCTGAAAGAAAAAGG


 


TGAAAACATCCTGCCTTACCTGACTAAAGCATGGGCTGACTTATGCAACGCATTTCTGC


 


AAGAGGCGAAATTTCTGTACAACAAATCCACTCCAACCTTCGATGACTACTTTGGCAAT


 


GCCTGGAAAAGTAGCAGTGGCCCGTTGCAGTTAGTGTTCGCTTATTTTGCGGTCGTTCA


 


AAACATTAAGAAGGAGGAGACGGAGAACCTTCAGAAGTACCACGACATTATTTCGTGG


 


CCAAGTTATATTTTTCGCTTATGTAATGATCTGGCCTCTGCGTCAGCCGAAATCGCTCG


 


GGGAGAGACCGCAAATTCTGTTTCTTGCTACATTAGAACTAAGGGCATCTCTGAGGAGT


 


TAGCAACGGAATCTGTTATGAATCTGATTGATGAGACTTGGAAAAAGATGAATAAAGA


 


GAAGGTGGGCGATTCACTGTTCGCTAAACAATTTGTAGAAACAGCTATTAATCTGGCTC


 


GTCAGTCACATTGCACGTATCATAATGGTGATGCACATACATCACCTGATGAGCTGACT


 


CGTAAACGTGTCTTATCTGTGATCACCGAACCGATCCTTCCGTTTGAAAGATGACTGCAG


 


DNA sequence of pEWL834


GTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCAT


 


CGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAG


 


GCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATA


 


TTCTGAAATGAGCTGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAG


 


CGGATAACAATTTCACACAGGAAACAGCGCCGCTGAGAAAAAGCGAAGCGGCACTGC


 


TCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGACCGGAATTATCGATTAACTT


 


TATTATTAAAAATTAAAGAGGTATATATTAATGTATCGATTAAATAAGGAGGAATAAA


 


CCATGGAAACCCGCAGAACCGCCAATTATGAACCCAATTCCTGGGATTACGATTACCTT


 


TTGAGTAGCGATAACGATGACGCCATAGAACTGTACAAAGATAAAGCCAAGAAACTTG


 


ATGCAGAAGTGCGTAGTAAAATTAATAACGAAAAAGCAGAATTTCTTACACAACTTGA


 


ACTCATTGATACTATCCAACGTCTCGGCCTCGGATACCGCTTCGAATCAGATATACGGC


 


GCGCTATCGATAGATATGTTTCATCGGGCGGATTCGAGGCAGTGGCTAAAACGTCGTTA


 


CACGCAACAGCACTCAGTTTTCGTTTGTTACGCCAACATGGATTCGAAGTGAGCCAAGA


 


GGTATTTTCGGGTTTCAAGGACCAAAATGGAAACTTTATGGAGAACTTGAAAGAGGAC


 


ATAAAGGCAATTCTTAGTCTCTATGAAGCGAGTTTCCTTGCTTTAGAAGGCGAGAATAT


 


ATTGGACGAAGCCAAGGTCTTTACAATCTCCCACCTGAAAGAGCTGAATGAGGAAAAA


 


ATTGGAAAAGACCTGGCCGACCAAGTGAATCATGCGCTGGAACTTCCTCTGCATCGGC


 


GTACCCAGCGTCTTGAAGCGGTATGGTCGATAGAAGCCTATCGCAAAAAGGAAGGAGC


 


GAATCGCGCATTACTGGAACTGGCGATCTTGGACTACAATATGGTCCAATCAGTCTACC


 


AACGCGATTTGCGCGAAACCTCCCGCTGGTGGCGCCGTGTAGGCTTAGCAACTAAATT


 


GCATTTTGCACGTGACCGGCTGATTGAATCTTTCTATTGGGCGGTCGGCGTTGCCTTTG


 


AGCCGCAATATTCTGACTGTCGCAAATCTGTGGCTAAAATGTTTTCATTCGTGACCATA


 


ATTGATGACATATATGATGTTTATGGGACGTTAGATGAGTTAGAGTTGTTTACCGACGC


 


AGTTGAACGCTGGGACGTTTCTGCCGTGGATGACCTCCCGGATTATATGAAATTATGCT


 


TTCTGGCCCTGTACAACACAATCAATGAGATAGCATACGACAATCTGAAAGAAAAAGG


 


TGAAAACATCCTGCCTTACCTGACTAAAGCATGGGCTGACTTATGCAACGCATTTCTGC


 


AAGAGGCGAAATTTCTGTACAACAAATCCACTCCAACCTTCGATGACTACTTTGGCAAT


 


GCCTGGAAAAGTAGCAGTGGCCCGTTGCAGTTAGTGTTCGCTTATTTTGCGGTCGTTCA


 


AAACATTAAGAAGGAGGAGACGGAGAACCTTCAGAAGTACCACGACATTATTTCGTGG


 


CCAAGTTATATTTTTCGCTTATGTAATGATCTGGCCTCTGCGTCAGCCGAAATCGCTCG


 


GGGAGAGACCGCAAATTCTGTTTCTTGCTACATTAGAACTAAGGGCATCTCTGAGGAGT


 


TAGCAACGGAATCTGTTATGAATCTGATTGATGAGACTTGGAAAAAGATGAATAAAGA


 


GAAGGTGGGCGATTCACTGTTCGCTAAACAATTTGTAGAAACAGCTATTAATCTGGCTC


 


GTCAGTCACATTGCACGTATCATAATGGTGATGCACATACATCACCTGATGAGCTGACT


 


CGTAAACGTGTCTTATCTGTGATCACCGAACCGATCCTTCCGTTTGAAAGATGACTGCA


 


GCTGGTACCATATGGGAATTCGAAGCTTTCTAGAACAAAAACTCATCTCAGAAGAGGA


 


TCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCT


 


TGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGC


 


AGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTG


 


ACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCC


 


CCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAG


 


ACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATC


 


CGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAC


 


GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCT


 


TTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCT


 


CATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT


 


ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTG


 


CTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGT


 


GGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA


 


GAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG


 


TGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG


 


GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT


 


TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACG


 


ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTC


 


GCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACAC


 


CACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT


 


ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGAC


 


CACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT


 


GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTA


 


TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAT


 


CGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCAT


 


ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATC


 


CTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCA


 


GACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTG


 


CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAG


 


CTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGT


 


CCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAT


 


ACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTT


 


ACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGG


 


GGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCT


 


ACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTA


 


TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAA


 


CGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT


 


GTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTA


 


CGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATT


 


CTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACG


 


ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTC


 


TCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCT


 


CTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGG


 


CTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCG


 


GCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTC


 


ACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCG


 


GCATGCATTTACGTTGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATA


 


GCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGAT


 


GTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAG


 


CCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTAC


 


ATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTG


 


CCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGC


 


GCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAG


 


CCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAAC


 


TATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGC


 


GTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGA


 


CGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTG


 


TTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATA


 


TCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATG


 


TCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCT


 


GGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTG


 


CGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTT


 


ATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGT


 


GGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCC


 


GTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCC


 


GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGG


 


GCAGTGAGCGCAACGCAATTAATGTGAGTTAGCGCGAATTGATCTG


 


DNA sequence of pEWL851


GTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCAT


 


CGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAG


 


GCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATA


 


TTCTGAAATGAGCTGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAG


 


CGGATAACAATTTCACACAGGAAACAGCGCCGCTGAGAAAAAGCGAAGCGGCACTGC


 


TCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGACCGGAATTATCGATTAACTT


 


TATTATTAAAAATTAAAGAGGTATATATTAATGTATCGATTAAATAAGGAGGAATAAA


 


CCATGGAAACCCGCAGAACCGCCAATTATGAACCCAATTCCTGGGATTACGATTACCTT


 


TTGAGTAGCGATAACGATGACGCCATAGAACTGTACAAAGATAAAGCCAAGAAACTTG


 


ATGCAGAAGTGCGTAGTAAAATTAATAACGAAAAAGCAGAATTTCTTACACAACTTGA


 


ACTCATTGATACTATCCAACGTCTCGGCCTCGGATACCGCTTCGAATCAGATATACGGC


 


GCGCTATCGATAGATATGTTTCATCGGGCGGATTCGAGGCAGTGGCTAAAACGTCGTTA


 


CACGCAACAGCACTCAGTTTTCGTTTGTTACGCCAACATGGATTCGAAGTGAGCCAAGA


 


GGTATTTTCGGGTTTCAAGGACCAAAATGGAAACTTTATGGAGAACTTGAAAGAGGAC


 


ATAAAGGCAATTCTTAGTCTCTATGAAGCGAGTTTCCTTGCTTTAGAAGGCGAGAATAT


 


ATTGGACGAAGCCAAGGTCTTTACAATCTCCCACCTGAAAGAGCTGAATGAGGAAAAA


 


ATTGGAAAAGACCTGGCCGACCAAGTGAATCATGCGCTGGAACTTCCTCTGCATCGGC


 


GTACCCAGCGTCTTGAAGCGGTATGGTCGATAGAAGCCTATCGCAAAAAGGAAGGAGC


 


GAATCGCGCATTACTGGAACTGGCGATCTTGGACTACAATATGGTCCAATCAGTCTACC


 


AACGCGATTTGCGCGAAACCTCCCGCTGGTGGCGCCGTGTAGGCTTAGCAACTAAATT


 


GCATTTTGCACGTGACCGGCTGATTGAATCTTTCTATTGGGCGGTCGGCGTTGCCTTTG


 


AGCCGCAATATTCTGACTGTCGCAAATCTGTGGCTAAAATGTTTTCATTCGTGACCATA


 


ATTGATGACATATATGATGTTTATGGGACGTTAGATGAGTTAGAGTTGTTTACCGACGC


 


AGTTGAACGCTGGGACGTTTCTGCCGTGGATGACCTCCCGGATTATATGAAATTATGCT


 


TTCTGGCCCTGTACAACACAATCAATGAGATAGCATACGACAATCTGAAAGAAAAAGG


 


TGAAAACATCCTGCCTTACCTGACTAAAGCATGGGCTGACTTATGCAACGCATTTCTGC


 


AAGAGGCGAAATTTCTGTACAACAAATCCACTCCAACCTTCGATGACTACTTTGGCAAT


 


GCCTGGAAAAGTAGCAGTGGCCCGTTGCAGTTAGTGTTCGCTTATTTTGCGGTCGTTCA


 


AAACATTAAGAAGGAGGAGACGGAGAACCTTCAGAAGTACCACGACATTATTTCGTGG


 


CCAAGTTATATTTTTCGCTTATGTAATGATCTGGCCTCTGCGTCAGCCGAAATCGCTCG


 


GGGAGAGACCGCAAATTCTGTTTCTTGCTACATTAGAACTAAGGGCATCTCTGAGGAGT


 


TAGCAACGGAATCTGTTATGAATCTGATTGATGAGACTTGGAAAAAGATGAATAAAGA


 


GAAGGTGGGCGATTCACTGTTCGCTAAACAATTTGTAGAAACAGCTATTAATCTGGCTC


 


GTCAGTCACATTGCACGTATCATAATGGTGATGCACATACATCACCTGATGAGCTGACT


 


CGTAAACGTGTCTTATCTGTGATCACCGAACCGATCCTTCCGTTTGAAAGATGACTGCA


 


TAAAGGAGGTAAAAAAACATGGTATCCTGTTCTGCGCCGGGTAAGATTTACCTGTTCG


 


GTGAACACGCCGTAGTTTATGGCGAAACTGCAATTGCGTGTGCGGTGGAACTGCGTAC


 


CCGTGTTCGCGCGGAACTCAATGACTCTATCACTATTCAGAGCCAGATCGGCCGCACCG


 


GTCTGGATTTCGAAAAGCACCCTTATGTGTCTGCGGTAATTGAGAAAATGCGCAAATCT


 


ATTCCTATTAACGGTGTTTTCTTGACCGTCGATTCCGACATCCCGGTGGGCTCCGGTCTG


 


GGTAGCAGCGCAGCCGTTACTATCGCGTCTATTGGTGCGCTGAACGAGCTGTTCGGCTT


 


TGGCCTCAGCCTGCAAGAAATCGCTAAACTGGGCCACGAAATCGAAATTAAAGTACAG


 


GGTGCCGCGTCCCCAACCGATACGTATGTTTCTACCTTCGGCGGCGTGGTTACCATCCC


 


GGAACGTCGCAAACTGAAAACTCCGGACTGCGGCATTGTGATTGGCGATACCGGCGTT


 


TTCTCCTCCACCAAAGAGTTAGTAGCTAACGTACGTCAGCTGCGCGAAAGCTACCCGG


 


ATTTGATCGAACCGCTGATGACCTCTATTGGCAAAATCTCTCGTATCGGCGAACAACTG


 


GTTCTGTCTGGCGACTACGCATCCATCGGCCGCCTGATGAACGTCAACCAGGGTCTCCT


 


GGACGCCCTGGGCGTTAACATCTTAGAACTGAGCCAGCTGATCTATTCCGCTCGTGCGG


 


CAGGTGCGTTTGGCGCTAAAATCACGGGCGCTGGCGGCGGTGGCTGTATGGTTGCGCT


 


GACCGCTCCGGAAAAATGCAACCAAGTGGCAGAAGCGGTAGCAGGCGCTGGCGGTAA


 


AGTGACTATCACTAAACCGACCGAGCAAGGTCTGAAAGTAGATTAAAGTCTAGTTAAA


 


GTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATA


 


CAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTA


 


GCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGA


 


TGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACG


 


AAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTC


 


TCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGG


 


AGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGC


 


CATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATAC


 


ATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA


 


AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGC


 


ATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAG


 


ATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCT


 


TGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTAT


 


GTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA


 


CTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATG


 


GCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGC


 


CAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC


 


ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC


 


CAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACT


 


ATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGG


 


CGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT


 


GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAG


 


ATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGA


 


TGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTG


 


TCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAA


 


AGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT


 


TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTT


 


TTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTT


 


TGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGC


 


GCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACT


 


CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGT


 


GGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGC


 


AGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTA


 


CACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGG


 


AGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGG


 


GAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTG


 


ACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCC


 


AGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTT


 


CCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATAC


 


CGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA


 


GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTG


 


CACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATC


 


GCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCC


 


TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAG


 


CTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAAT


 


TCGCGCGCGAAGGCGAAGCGGCATGCATTTACGTTGACACCATCGAATGGTGCAAAAC


 


CTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTG


 


AAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTC


 


CCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGC


 


GGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAA


 


CAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAAT


 


TGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATG


 


GTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAAC


 


GCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAA


 


GCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAA


 


CAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCAT


 


TGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTG


 


CGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAAC


 


GGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGA


 


GGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATG


 


CGCCAATCGCATTGTACGTATCATAATGGAGATGCGCATACTTCGCCTGACGAATTAAC


 


CCGCAAACGCGTACTGTCAGTGATCACTGAACCGATACTGCCATTGGAACGGTGACTG


 


CAGCTGGTACCATATGGGAATTCGAAGCTTTCTAGAACAAAAACTCATCTCAGAAGAG


 


GATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAG


 


CTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACG


 


CAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCT


 


GACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTC


 


CCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAA


 


GACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT


 


CCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGA


 


CGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCC


 


TTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGC


 


TCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAG


 


TATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT


 


GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAG


 


TGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA


 


GAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG


 


TGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG


 


GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT


 


TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACG


 


ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTC


 


GCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACAC


 


CACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT


 


ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGAC


 


CACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT


 


GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTA


 


TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAT


 


CGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCAT


 


ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATC


 


CTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCA


 


GACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTG


 


CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAG


 


CTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGT


 


CCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAT


 


ACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTT


 


ACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGG


 


GGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCT


 


ACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTA


 


TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAA


 


CGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT


 


GTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTA


 


CGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATT


 


CTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACG


 


ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTC


 


TCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCT


 


CTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGG


 


CTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCG


 


GCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTC


 


ACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCG


 


GCATGCATTTACGTTGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATA


 


GCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGAT


 


GTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAG


 


CCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTAC


 


ATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTG


 


CCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGC


 


GCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAG


 


CCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAAC


 


TATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGC


 


GTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGA


 


CGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTG


 


TTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATA


 


TCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATG


 


TCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCT


 


GGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTG


 


CGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTT


 


ATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGT


 


GGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCC


 


GTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCC


 


GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGG


 


GCAGTGAGCGCAACGCAATTAATGTGAGTTAGCGCGAATTGATCTG


 


DNA sequence of pEWL907


GTTTGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCAT


 


CGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAG


 


GCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATA


 


TTCTGAAATGAGCTGTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAG


 


CGGATAACAATTTCACACAGGAAACAGCGCCGCTGAGAAAAAGCGAAGCGGCACTGC


 


TCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGACCGGAATTATCGATTAACTT


 


TATTATTAAAAATTAAAGAGGTATATATTAATGTATCGATTAAATAAGGAGGAATAAA


 


CCATGGAAACCCGCAGAACCGCCAATTATGAACCCAATTCCTGGGATTACGATTACCTT


 


TTGAGTAGCGATAACGATGACGCCATAGAACTGTACAAAGATAAAGCCAAGAAACTTG


 


ATGCAGAAGTGCGTAGTAAAATTAATAACGAAAAAGCAGAATTTCTTACACAACTTGA


 


ACTCATTGATACTATCCAACGTCTCGGCCTCGGATACCGCTTCGAATCAGATATACGGC


 


GCGCTATCGATAGATATGTTTCATCGGGCGGATTCGAGGCAGTGGCTAAAACGTCGTTA


 


CACGCAACAGCACTCAGTTTTCGTTTGTTACGCCAACATGGATTCGAAGTGAGCCAAGA


 


GGTATTTTCGGGTTTCAAGGACCAAAATGGAAACTTTATGGAGAACTTGAAAGAGGAC


 


ATAAAGGCAATTCTTAGTCTCTATGAAGCGAGTTTCCTTGCTTTAGAAGGCGAGAATAT


 


ATTGGACGAAGCCAAGGTCTTTACAATCTCCCACCTGAAAGAGCTGAATGAGGAAAAA


 


ATTGGAAAAGACCTGGCCGACCAAGTGAATCATGCGCTGGAACTTCCTCTGCATCGGC


 


GTACCCAGCGTCTTGAAGCGGTATGGTCGATAGAAGCCTATCGCAAAAAGGAAGGAGC


 


GAATCGCGCATTACTGGAACTGGCGATCTTGGACTACAATATGGTCCAATCAGTCTACC


 


AACGCGATTTGCGCGAAACCTCCCGCTGGTGGCGCCGTGTAGGCTTAGCAACTAAATT


 


GCATTTTGCACGTGACCGGCTGATTGAATCTTTCTATTGGGCGGTCGGCGTTGCCTTTG


 


AGCCGCAATATTCTGACTGTCGCAAATCTGTGGCTAAAATGTTTTGTTTCGTGACCATA


 


ATTGATGACATATATGATGTTTATGGGACGTTAGATGAGTTAGAGTTGTTTACCGACGC


 


AGTTGAACGCTGGGACGTTTCTGCCGTGGATGACCTCCCGGATTATATGAAATTATGCT


 


TTCTGGCCCTGTACAACACAATCAATGAGATAGCATACGACAATCTGAAAGAAAAAGG


 


TGAAAACATCCTGCCTTACCTGACTAAAGCATGGGCTGACTTATGCAACGCATTTCTGC


 


AAGAGGCGAAATTTCTGTACAACAAATCCACTCCAACCTTCGATGACTACTTTGGCAAT


 


GCCTGGAAAAGTAGCAGTGGCCCGTTGCAGTTAGTGTTCGCTTATTTTGCGGTCGTTCA


 


AAACATTAAGAAGGAGGAGACGGAGAACCTTCAGAAGTACCACGACATTATTTCGTGG


 


CCAAGTTATATTTTTCGCTTATGTAATGATCTGGCCTCTGCGTCAGCCGAAATCGCTCG


 


GGGAGAGACCGCAAATTCTGTTTCTTGCTACATTAGAACTAAGGGCATCTCTGAGGAGT


 


TAGCAACGGAATCTGTTATGAATCTGATTGATGAGACTTGGAAAAAGATGAATAAAGA


 


GAAGGTGGGCGATTCACTGTTCGCTAAACAATTTGTAGAAACAGCTATTAATCTGGCTC


 


GTCAGTCACATTGCACGTATCATAATGGTGATGCACATACATCACCTGATGAGCTGACT


 


CGTAAACGTGTCTTATCTGTGATCACCGAACCGATCCTTCCGTTTGAAAGATGACTGCA


 


GCTGGTACCATATGGGAATTCGAAGCTTTCTAGAACAAAAACTCATCTCAGAAGAGGA


 


TCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCT


 


TGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGC


 


AGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTG


 


ACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCC


 


CCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAG


 


ACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATC


 


CGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAC


 


GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCT


 


TTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCT


 


CATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT


 


ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTG


 


CTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGT


 


GGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA


 


GAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG


 


TGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG


 


GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT


 


TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACG


 


ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTC


 


GCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACAC


 


CACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT


 


ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGAC


 


CACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT


 


GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTA


 


TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAT


 


CGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCAT


 


ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATC


 


CTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCA


 


GACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTG


 


CTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAG


 


CTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGT


 


CCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAT


 


ACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTT


 


ACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGG


 


GGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCT


 


ACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTA


 


TCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAA


 


CGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTT


 


GTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTA


 


CGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATT


 


CTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACG


 


ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTC


 


TCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCT


 


CTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGG


 


CTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCG


 


GCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTC


 


ACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCG


 


GCATGCATTTACGTTGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATA


 


GCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGAT


 


GTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAG


 


CCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTAC


 


ATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTG


 


CCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGC


 


GCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAG


 


CCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAAC


 


TATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGC


 


GTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGA


 


CGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTG


 


TTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATA


 


TCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATG


 


TCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCT


 


GGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTG


 


CGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTT


 


ATATCCCGCCGTCAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGT


 


GGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCC


 


GTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCC


 


GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGG


 


GCAGTGAGCGCAACGCAATTAATGTGAGTTAGCGCGAATTGATCTG






Example 4
Crystal Structure of MEA P. alba A3T S288C
The S288C mutation was introduced into the MD09-163 vector (previously described) by QuikChange (Stratagene) mutagenesis using the primers listed in Table 10. Mutagenesis was carried out according to the manufacturer's recommended protocol. PCR products were transformed into chemically competent Top10 E. coli (Invitrogen) cells, and positive transformants were selected for plasmid isolation and complete sequencing. A single plasmid, pDW 196 (Table 11, FIG. 23), was verified and used for additional mutagenesis. The A3T mutation was introduced in the pDW 196 vector by the same procedure as described above. The fully verified plasmid, pDW204 (Table 11, FIG. 24), was transformed into chemically competent BL21 DE3 pLysS cells (Invitrogen) according to the manufacturer's recommended protocol. This strain, DW614 was used for purification and subsequent crystallization for structural analysis of the MEA P. alba IspS A3T S288C variant.







TABLE 10 







Primers used in this study








Primer
Sequence





HgS pET QC
ACATATGGAAACGCGTCGTTCTGCGAACTACGA


A3T Forward



 


HgS pET QC
CAGAACGACGCGTTTCCATATGTATATCTCCTT


A3T Reverse



 


HgS QC S288C
AAAAATGTTTTGTTTCGTAACCATTATCGACGA


Forward



 


HgS QC S288C
TGGTTACGAAACAAAACATTTTTGCGACGGAGT


Reverse
















TABLE 11







Plasmids/Strains used in this study









Plasmid/




Strain
Resistance
Description





pDW196
Kan
pET MEA P. alba IspS S288C TEV 6X His


pDW204
Kan
pET MEA P. alba IspS A3T S288C TEV 6X His


DW614
Chlor,
BL21 (DE3), pLysS, pDW204 = pET MEA



Kan
P. alba IspS A3T S288C TEV 6X His

















Amino acid sequence of MEA P. alba IspS S288C TEV 6XHis



MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFLTLLELID


 


NVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQHGFEVSQEAFSGF


 


KDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKIGKELAEQVN


 


HALELPLHRRTQRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRETSRWWR


 


RVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFCFVTIIDDIYDVYGTLDEL


 


ELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDKGENILPYLTKAWADLC


 


NAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIKKEEIENLQKYHDTI


 


SRPSHIFRLCNDLASASAEIARGETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKE


 


KLGGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPILPFERENLYFQG


 


LEHHHHHH


 


DNA sequence of pDW196 harboring MEA P. alba IspS S288C TEV 6XHis


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctag


 


cgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttc


 


cgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcg


 


ccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatt


 


tataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgttt


 


acaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaa


 


ttaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttc


 


gatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaa


 


tgtaatgaaggagaaaactcaccgaggcagttccataggatggcaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttat


 


gcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgc


 


gcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatc


 


aacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcag


 


gagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacg


 


ctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcg


 


agcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacac


 


cccttgtattactgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccc


 


cgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtgg


 


tttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtag


 


ccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcga


 


taagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccca


 


gcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacagg


 


tatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcg


 


ccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcc


 


tggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgat


 


accgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatct


 


gtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgcta


 


cgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagaca


 


agctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgt


 


ggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaag


 


cgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaa


 


acgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgc


 


ggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcg


 


atgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgc


 


tcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagc


 


ctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttg


 


gtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaa


 


gcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatag


 


tcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaactt


 


acattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagagg


 


cggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagt


 


tgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggt


 


atcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttgg


 


caaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttcc


 


gctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacag


 


cgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtct


 


ggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatc


 


agcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggc


 


acccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacg


 


actgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaa


 


acgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacatt


 


caccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctct


 


cccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagat


 


ggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccc


 


catcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatc


 


tcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaag


 


gagatatacatatggaagctcgtcgttctgcgaactacgaacctaacagctgggactatgattacctgctgtcctccgacacggacgagtcc


 


atcgaagtatacaaagacaaagcgaaaaagctggaagccgaagttcgtcgcgagattaataacgaaaaagcagaatttctgaccctgctgga


 


actgattgacaacgtccagcgcctgggcctgggttaccgtttcgagtctgatatccgtggtgcgctggatcgcttcgtttcctccggcggct


 


tcgatgcggtaaccaagacttccctgcacggtacggcactgtctttccgtctgctgcgtcaacacggttttgaggtttctcaggaagcgttc


 


agcggcttcaaagaccaaaacggcaacttcctggagaacctgaaggaagatatcaaagctatcctgagcctgtacgaggccagcttcctggc


 


tctggaaggcgaaaacatcctggacgaggcgaaggttttcgcaatctctcatctgaaagaactgtctgaagaaaagatcggtaaagagctgg


 


cagaacaggtgaaccatgcactggaactgccactgcatcgccgtactcagcgtctggaagcagtatggtctatcgaggcctaccgtaaaaag


 


gaggacgcgaatcaggttctgctggagctggcaattctggattacaacatgatccagtctgtataccagcgtgatctgcgtgaaacgtcccg


 


ttggtggcgtcgtgtgggtctggcgaccaaactgcactttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagcattcg


 


aaccgcaatactccgactgccgtaactccgtcgcaaaaatgttttgtttcgtaaccattatcgacgatatctacgatgtatacggcaccctg


 


gacgaactggagctgtttactgatgcagttgagcgttgggacgtaaacgccatcaacgacctgccggattacatgaaactgtgctttctggc


 


tctgtataacactattaacgaaatcgcctacgacaacctgaaagataaaggtgagaacatcctgccgtatctgaccaaagcctgggctgacc


 


tgtgcaacgctttcctgcaagaagccaagtggctgtacaacaaatctactccgacctttgacgactacttcggcaacgcatggaaatcctct


 


tctggcccgctgcaactggtgttcgcttacttcgctgtcgtgcagaacattaaaaaggaagagatcgaaaacctgcaaaaataccatgacac


 


catctctcgtccttcccatatcttccgtctgtgcaatgacctggctagcgcgtctgcggaaattgcgcgtggtgaaaccgcaaatagcgttt


 


cttgttacatgcgcactaaaggtatctccgaagaactggctaccgaaagcgtgatgaatctgatcgatgaaacctggaaaaagatgaacaag


 


gaaaaactgggtggtagcctgttcgcgaaaccgttcgtggaaaccgcgatcaacctggcacgtcaatctcactgcacttatcataacggcga


 


cgcgcatacctctccggatgagctgacccgcaaacgcgttctgtctgtaatcactgaaccgattctgccgtttgaacgcgaaaacctgtatt


 


ttcagggcctcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgag


 


caataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat


 


Amino acid sequence of MEA P. alba IspS A3T S288C TEV 6XHis


METRRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFLTLLELID


 


NVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQHGFEVSQEAFSGF


 


KDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKIGKELAEQVN


 


HALELPLHRRTQRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRETSRWWR


 


RVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFCFVTIIDDIYDVYGTLDEL


 


ELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDKGENILPYLTKAWADLC


 


NAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIKKEEIENLQKYHDTI


 


SRPSHIFRLCNDLASASAEIARGETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKE


 


KLGGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPILPFERENLYFQG


 


LEHHHHHH


 


The sequence of SEQ ID NO: 2 is


METRRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFLTLLELID


 


NVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQHGFEVSQEAFSGF


 


KDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKIGKELAEQVN


 


HALELPLHRRTQRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRETSRWWR


 


RVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFCFVTIIDDIYDVYGTLDEL


 


ELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDKGENILPYLTKAWADLC


 


NAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIKKEEIENLQKYHDTI


 


SRPSHIFRLCNDLASASAEIARGETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKE


 


KLGGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPILPFERENLYFQGLE


 


DNA sequence of pDW204 harboring MEA P. alba IspS A3T S288C TEV 6XHis


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctag


 


cgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttc


 


cgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcg


 


ccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatt


 


tataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgttt


 


acaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaa


 


ttaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttc


 


tgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaataca


 


acctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttat


 


gcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgc


 


gcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatc


 


aacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcag


 


gagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacg


 


ctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcg


 


agcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacac


 


cccttgtattactgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccc


 


cgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtgg


 


tttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtag


 


ccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcga


 


taagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccca


 


gcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacagg


 


tatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcg


 


ccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcc


 


tggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgat


 


accgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatct


 


gtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgcta


 


cgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagaca


 


agctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgt


 


ggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaag


 


cgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaa


 


acgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgc


 


ggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcg


 


atgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgc


 


tcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagc


 


ctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttg


 


gtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaa


 


gcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatag


 


tcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaactt


 


acattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagagg


 


cggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagt


 


tgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggt


 


atcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttgg


 


caaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttcc


 


gctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacag


 


cgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtct


 


ggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatc


 


agcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggc


 


acccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacg


 


actgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaa


 


acgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacatt


 


caccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctct


 


cccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagat


 


ggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccc


 


catcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatc


 


tcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaag


 


gagatatacatatggaaacgcgtcgttctgcgaactacgaacctaacagctgggactatgattacctgctgtcctccgacacggacgagtcc


 


atcgaagtatacaaagacaaagcgaaaaagctggaagccgaagttcgtcgcgagattaataacgaaaaagcagaatttctgaccctgctgga


 


actgattgacaacgtccagcgcctgggcctgggttaccgtttcgagtctgatatccgtggtgcgctggatcgcttcgtttcctccggcggct


 


tcgatgcggtaaccaagacttccctgcacggtacggcactgtctttccgtctgctgcgtcaacacggttttgaggtttctcaggaagcgttc


 


agcggcttcaaagaccaaaacggcaacttcctggagaacctgaaggaagatatcaaagctatcctgagcctgtacgaggccagcttcctggc


 


tctggaaggcgaaaacatcctggacgaggcgaaggttttcgcaatctctcatctgaaagaactgtctgaagaaaagatcggtaaagagctgg


 


cagaacaggtgaaccatgcactggaactgccactgcatcgccgtactcagcgtctggaagcagtatggtctatcgaggcctaccgtaaaaag


 


gaggacgcgaatcaggttctgctggagctggcaattctggattacaacatgatccagtctgtataccagcgtgatctgcgtgaaacgtcccg


 


ttggtggcgtcgtgtgggtctggcgaccaaactgcactttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagcattcg


 


aaccgcaatactccgactgccgtaactccgtcgcaaaaatgttttgtttcgtaaccattatcgacgatatctacgatgtatacggcaccctg


 


gacgaactggagctgtttactgatgcagttgagcgttgggacgtaaacgccatcaacgacctgccggattacatgaaactgtgctttctggc


 


tctgtataacactattaacgaaatcgcctacgacaacctgaaagataaaggtgagaacatcctgccgtatctgaccaaagcctgggctgacc


 


tgtgcaacgctttcctgcaagaagccaagtggctgtacaacaaatctactccgacctttgacgactacttcggcaacgcatggaaatcctct


 


tctggcccgctgcaactggtgttcgcttacttcgctgtcgtgcagaacattaaaaaggaagagatcgaaaacctgcaaaaataccatgacac


 


catctctcgtecttcccatatcttccgtctgtgcaatgacctggctagcgcgtctgeggaaattgcgcgtggtgaaaccgcaaatagcgttt


 


cttgttacatgcgcactaaaggtatctccgaagaactggctaccgaaagcgtgatgaatctgatcgatgaaacctggaaaaagatgaacaag


 


gaaaaactgggtggtagcctgttcgcgaaaccgttcgtggaaaccgcgatcaacctggcacgtcaatctcactgcacttatcataacggcga


 


cgcgcatacctctccggatgagctgacccgcaaacgcgttctgtctgtaatcactgaaccgattctgccgtttgaacgcgaaaacctgtatt


 


ttcagggcctcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgag


 


caataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat






Expression and Purification of MEA P. Alba A3T S288C

Expression of 6×His-tagged MEA P. Alba A3T S288C

N-terminally 6×His-tagged MEA P. Alba A3T S288C was expressed and purified from strain DW614. The growth procedure is suitable for histidine tagged enzymes expressed in BL21(λDE3)pLysS cells. A 10 ml of overnight culture was prepared for each 1 L of planned growth. The appropriate antibiotics (50 mg/ml kanamycin, 50 mg/ml chloramphenicol) was added to 10 ml of LB medium in a 25 ml flask and was inoculated with 1 colony from a fresh plate of cells or directly from glycerol frozen cell stock. Cultures were grown at 30° C. overnight with shaking at  ̃220 rpm. Day cultures were prepared in 1 liter of LB medium with appropriate antibiotics for each culture. Each 1 L day culture was inoculated with 10 ml of overnight culture and grown at 30-37° C. with shaking at  ̃220 rpm until the OD600 reached  ̃0.4-0.6. Day cultures were then induced with 400 μM IPTG and allowed to continue growing at 30° C. with shaking at 220 rpm for  ̃5-6 hours. Cells were then harvested by centrifugation at 10,000×g for 10 min, 4° C. Following Harvest, cells were used directly or stored at −80° C. until ready to process.
Purification of 6×his-Tagged MEA P. Alba A3T S288C
For purification of histidine tagged enzymes from BL21(λDE3)pLysS cells, cells were gently resuspended in fresh Lysis buffer (Lysis buffer: Ni wash buffer+0.5 mM PMST, 0.01% Tween-20, 1 mg/ml lysozyme, 0.2 mg/ml DNaseI; Ni wash buffer: 50 mM NaH2PO4, 300 mM NaCl, 20 mM Imidazole, pH 8.0). Approximately 40-50 ml of lysis buffer was used per 1 L of cell pellet. Cells were then incubated on ice for approximately 30 min. The cell suspension was then lysed fully by passing 2-3 times through a french pressure cell (large french press cell at 1200 psi/High setting) until lysate started to look clear. A sample of the lysate was saved for activity assay and gel analysis ( ̃100 μl). The lysate was then clarified by centrifuging the lysate at 30,000×g for 30 min, 4° C. in a Sorvall Discovery 90SE ultracentrifuge. The supernatant was removed and retained. A sample of the “clarified lysate” was saved for activity assay and gel analysis ( ̃100 μl).
The clarified lysate was run over HisTrap HP columns (GE healthcare) using a gradient from 0-100% Ni buffer B. Following loading of the lysate on the column, the column was washed with Ni wash buffer (50 mM NaH2PO4, 300 mm NaCl, 20 mM imidazole, ph 8.0). The his-tagged IspS was then eluted from the column using a gradient from 0-100% Ni elution buffer (50 mM NaH2PO4, 300 mM NaCl, 500 mM imidazole, ph 8.0) and fractions containing the his-tagged IspS were collected. The column was then washed with Ni stripping buffer (20 mM NaH2PO4, 0.5 m NaCl, 50 mM EDTA, ph 7.4). Samples were then analyzed by SDS-PAGE gel (4-12% gel NUPAGE, Invitrogen) according to manufacturer's directions. Desired fractions were concentrated on spin filters (Vivaspin-20, Sartoris,) and then desalted over a Hi Prep 26/10 Desalting column (GE heathcare) packed with Sephadex G25 resin. The G-25 buffer consisted of 50 mM HEPES, 50 mM NaCl, and 1 mM DTT, pH 7.4. Fractions were then analyzed and concentrated. The samples were then stored at −80° C.
TEV Cleavage of MEA P. Alba A3T S288C from Strain DW614
Strain DW614 is described above. Digestion was performed with TurboTEV Protease from Eton Bioscience Inc. One unit of TurboTEV per 10 μg of purified protein was used. The digest was performed at 4° C. overnight. Samples were passed through another Ni column equilibrated in the Ni buffer to remove uncleaved enzyme, tag, TurboTEV protease (which is also tagged), and impurities. The Ni column pass though and washes were analyzed using SDS-PAGE gel (NUPAGE, Invitrogen) and DMAPP activity assays. Samples containing pure enzyme were pooled and desalted into 50 mM NaCl pH 7.4 buffer containing 1 mM DTT and stored at −80° C.
Crystal Structure Determination
Construct DW614 was purified as described and a concentrated protein solution was then prepared for surveying possible crystallization conditions. The construct was purified independently and surveyed as described below. At a minimum, the construct was surveyed using the following commercial screens: the Crystal Screen from Hampton Research (Aliso Viejo, Calif.) and the JCSG+ Suite from Qiagen (Valencia, Calif.).
Initial crystallization screens were setup using the Crystal Screen from Hampton Research and the JCSG+ Suite from Qiagen. Crystals from this construct were observed in numerous conditions; optimization included 50 variations of pH, precipitating agents, and concentrations. From the optimization experiments, five different DW614 crystals were screened for diffraction. A crystal composed of MEA P. Alba A3T S288C was obtained that diffracted to 2.5 Å. The large, rod-shaped crystals belong to the tetragonal space group P43212, and have unit cell dimensions a=b=156.84, c=143.41. The crystals were grown by mixing 2 μL of protein (9 mg/ml protein) with 2 μL of precipitant solution [0.2 M Malic Acid, pH 7.0, 8% (wt/vol) Polyethylene glycol 8000] and equilibrated against 500 μL of precipitant. Prior to flash-freezing the crystal in liquid nitrogen, the crystals were cryoprotected by swishing through 0.2 M Malic Acid, pH 7.0, 8% (wt/vol) Polyethylene glycol 8000, and 25% (wt/vol) ethylene glycol.
Single-wavelength anomalous dispersion data (SAD) were collected to verify the presence of the serine to cysteine substitution. Data were integrated using Mosflm (Leslie, A. (1998) J. of Appl. Crystallography 30, 1036-1040) and scaled using SCALA (Collaborative Computational Project, N. (1994) Acta Crystallographica Section D 50, 760-763). The data were phased with MOLREP (Vagin, A., and Teplyakov, A. (1997) J. of Appl. Crystallography 30, 1022-1025), using the previously determined structure of Isoprene synthase from P. alba as the starting model (US2009/0076743). The crystal contains one dimer in the asymmetric unit with a solvent content of 63%. The SAD data were phased using Phaser EP (Read, R. J., and McCoy, A. J. (2011) Acta Crystallographica Section D 67, 338-344). The resulting anomalous difference maps clearly indicate the presence of cysteine at position 288 (FIG. 19).
The same crystal was sent to Stanford Synchrotron Radiation Laboratory, and data were collected at Beamline 11-1 to 1.98 Å resolution. Data were processed as described above and were used for model refinement. Refinement with Refmac5 (Collaborative Computational Project, N. (1994) Acta Crystallographica Section D 50, 760-763) was used with iterative manual rebuilding steps using the visualization program Coot (Emsley, P., et al. (2010) Acta Crystallographica Section D 66, 486-501). During refinement, the geometry of the protein was checked using Molprobity (Davis, I. W., et al. (2007) Nucl. Acids Res., 35:W375-W383). The current model has an RWork value of 17.8% and an RFree value of 20.9%.
The structure consists of a homo dimer in the asymmetric unit (FIG. 20). Each monomer is comprised of two helical domains, a C-terminal domain containing the active site and an N-terminal domain with unknown function. Structural alignment of the wild type IspS and MEA P. Alba A3T S288C shows that the overall fold is unchanged, though the variant structure contains a few residues that were disordered in the previous structure (FIG. 21). The replacement of serine with cysteine does not result in any local structural perturbations (FIG. 22). Coordinates are provided in Appendix A.
Example 5
Differences Between P. alba and Salix Spp. IspS Enzymes
Amino acid differences between MEA P. alba and Salix spp. IspS enzymes are listed in Table 12. Charge changes for individual amino acid substitutions are shown in Table 12, as well as % surface accessibility of each position in the crystal structure of MEA P. alba. Surface accessibility was calculated using the program MOE, which is written and supported by the Chemical Computing Group, Inc. An estimate for the water-accessible surface area of each residue was determined using a probe having a specified radius. The estimate was then compared against a library of peptides and the ratio between them was reported as the percent surface accessibility. Positions where the sequence of S. alba differs from P. alba are shown in FIG. 25. Positions where the sequence of S. babylonica differs from P. alba are shown in FIG. 26.







TABLE 12







Differences between P. alba and Salix spp. isoprene synthase enzymes. Charge changes


and surface accessibilities of positions in the P. alba MEA crystal structure are indicated.

















S. alba Δ
S. babylonica
% Surface


Position
MEA P. alba
S. alba
S. babylonica
Charge
Δ Charge
Accessibility
















3
A
T
T
0
0
Not calc.


6
S

T

0
Not calc.


24
T

N

0
84


25
D
E

0

55


26
E
D
D
0
0
86


27
S
A
A
0
0
65


30
V

L

0
60


39
E
D
D
0
0
13


44
R
S
S
−1
−1
55


45
E
K
K
2
2
31


50
K
T

−1

89


56
L
Q
Q
0
0
5


62
N
T
T
0
0
13


63
V
I
I
0
0
0


78
G
R
R
1
1
29


80
L

I

0
0


83
F

Y

0
1


90
D
E
E
0
0
54


93
T
A
A
0
0
8


98
H
Q

0

10


99
G
A
A
0
0
0


118
A
V
V
0
0
10


120
S
N

0

52


130
L
M
M
0
0
33


132
N
D

−1

63


140
I
L

0

1


144
Y
H

0

2


164
A
T
T
0
0
0


172
S
N
N
0
0
47


179
E
D
D
0
0
46


180
L
M

0

28


181
A
V

0

8


182
E

D

0
40


213
D

G

1
83


216
Q
R
R
1
1
45


217
V

A

0
20


229
I
V
V
0
0
0


281
N
I
K
0
1
10


304
D
E

0

66


319
N
S
S
0
0
37


321
I

V

0
3


322
N
D
D
−1
−1
54


349
D
E
E
0
0
64


374
K
R

0

23


375
W
F
F
0
0
14


385
D
S

1

47


417
I
T
T
0
0
6


421
Q
L

0

23


426
T
I
I
0
0
0


429
R
W
W
−1
−1
5


432
H
Y
Y
0
0
6


460
M

I

0
21


490
L

V

0
19


492
G
D
D
−1
−1
89


498
P
H
Q
0
0
57


542
F
L

0

17









Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention, which are obvious to those skilled in the relevant fields, are intended to be within the scope of the following claims.







APPENDIX A





P. alba 3T288C coordinates



























ATOM
1
N
SER
A
6
57.986
−35.273
−30.156
1.00
72.58
A
N


ATOM
2
CA
SER
A
6
58.480
−33.880
−30.355
1.00
68.61
A
C


ATOM
3
CB
SER
A
6
57.316
−32.894
−30.340
1.00
61.40
A
C


ATOM
4
OG
SER
A
6
57.743
−31.589
−30.698
1.00
58.19
A
O


ATOM
5
C
SER
A
6
59.509
−33.500
−29.283
1.00
75.11
A
C


ATOM
6
O
SER
A
6
59.271
−33.694
−28.080
1.00
66.06
A
O


ATOM
7
N
ALA
A
7
60.650
−32.967
−29.734
1.00
55.83
A
N


ATOM
8
CA
ALA
A
7
61.745
−32.563
−28.846
1.00
37.95
A
C


ATOM
9
CB
ALA
A
7
63.101
−32.683
−29.571
1.00
35.03
A
C


ATOM
10
C
ALA
A
7
61.520
−31.137
−28.346
1.00
34.22
A
C


ATOM
11
O
ALA
A
7
60.884
−30.305
−29.015
1.00
33.25
A
O


ATOM
12
N
ASN
A
8
62.042
−30.855
−27.159
1.00
31.53
A
N


ATOM
13
CA
ASN
A
8
62.004
−29.521
−26.602
1.00
41.19
A
C


ATOM
14
CB
ASN
A
8
61.256
−29.534
−25.259
1.00
43.35
A
C


ATOM
15
CG
ASN
A
8
61.257
−28.175
−24.574
1.00
38.99
A
C


ATOM
16
OD1
ASN
A
8
61.277
−27.124
−25.221
1.00
47.85
A
O


ATOM
17
ND2
ASN
A
8
61.227
−28.195
−23.246
1.00
65.44
A
N


ATOM
18
C
ASN
A
8
63.431
−29.020
−26.417
1.00
39.96
A
C


ATOM
19
O
ASN
A
8
64.147
−29.495
−25.529
1.00
46.00
A
O


ATOM
20
N
TYR
A
9
63.845
−28.090
−27.272
1.00
34.27
A
N


ATOM
21
CA
TYR
A
9
65.157
−27.460
−27.165
1.00
35.68
A
C


ATOM
22
CB
TYR
A
9
65.835
−27.435
−28.549
1.00
30.73
A
C


ATOM
23
CG
TYR
A
9
66.034
−28.818
−29.146
1.00
27.98
A
C


ATOM
24
CD1
TYR
A
9
66.744
−29.815
−28.449
1.00
22.86
A
C


ATOM
25
CE1
TYR
A
9
66.919
−31.070
−28.996
1.00
23.00
A
C


ATOM
26
CZ
TYR
A
9
66.392
−31.361
−30.263
1.00
19.46
A
C


ATOM
27
OH
TYR
A
9
66.559
−32.617
−30.842
1.00
19.25
A
O


ATOM
28
CE2
TYR
A
9
65.680
−30.412
−30.944
1.00
20.38
A
C


ATOM
29
CD2
TYR
A
9
65.515
−29.138
−30.403
1.00
20.74
A
C


ATOM
30
C
TYR
A
9
65.053
−26.029
−26.583
1.00
39.07
A
C


ATOM
31
O
TYR
A
9
66.045
−25.297
−26.512
1.00
42.20
A
O


ATOM
32
N
GLU
A
10
63.862
−25.632
−26.144
1.00
43.68
A
N


ATOM
33
CA
GLU
A
10
63.653
−24.239
−25.734
1.00
51.14
A
C


ATOM
34
CB
GLU
A
10
62.175
−23.842
−25.891
1.00
63.92
A
C


ATOM
35
CG
GLU
A
10
61.624
−24.061
−27.347
1.00
73.92
A
C


ATOM
36
CD
GLU
A
10
62.612
−23.648
−28.477
1.00
85.85
A
C


ATOM
37
OE1
GLU
A
10
63.165
−22.528
−28.397
1.00
73.86
A
O


ATOM
38
OE2
GLU
A
10
62.831
−24.435
−29.443
1.00
75.20
A
O


ATOM
39
C
GLU
A
10
64.198
−24.048
−24.323
1.00
37.85
A
C


ATOM
40
O
GLU
A
10
64.234
−24.992
−23.557
1.00
32.89
A
O


ATOM
41
N
PRO
A
11
64.689
−22.845
−24.000
1.00
44.76
A
N


ATOM
42
CA
PRO
A
11
65.387
−22.665
−22.723
1.00
46.27
A
C


ATOM
43
CB
PRO
A
11
66.119
−21.335
−22.922
1.00
54.27
A
C


ATOM
44
CG
PRO
A
11
65.182
−20.535
−23.821
1.00
55.02
A
C


ATOM
45
CD
PRO
A
11
64.500
−21.564
−24.721
1.00
51.34
A
C


ATOM
46
C
PRO
A
11
64.411
−22.569
−21.533
1.00
42.77
A
C


ATOM
47
O
PRO
A
11
63.275
−22.169
−21.729
1.00
45.64
A
O


ATOM
48
N
ASN
A
12
64.852
−22.959
−20.334
1.00
36.56
A
N


ATOM
49
CA
ASN
A
12
64.098
−22.713
−19.087
1.00
37.47
A
C


ATOM
50
CB
ASN
A
12
64.272
−23.862
−18.096
1.00
38.59
A
C


ATOM
51
CG
ASN
A
12
64.111
−25.195
−18.745
1.00
49.28
A
C


ATOM
52
OD1
ASN
A
12
62.997
−25.608
−19.041
1.00
50.88
A
O


ATOM
53
ND2
ASN
A
12
65.227
−25.877
−19.000
1.00
52.57
A
N


ATOM
54
C
ASN
A
12
64.496
−21.418
−18.389
1.00
38.56
A
C


ATOM
55
O
ASN
A
12
65.646
−20.955
−18.485
1.00
31.10
A
O


ATOM
56
N
SER
A
13
63.541
−20.847
−17.650
1.00
38.39
A
N


ATOM
57
CA
SER
A
13
63.768
−19.565
−16.967
1.00
47.84
A
C


ATOM
58
CB
SER
A
13
62.462
−19.064
−16.345
1.00
42.98
A
C


ATOM
59
OG
SER
A
13
61.847
−20.123
−15.628
1.00
43.24
A
O


ATOM
60
C
SER
A
13
64.844
−19.690
−15.885
1.00
39.53
A
C


ATOM
61
O
SER
A
13
65.430
−18.690
−15.484
1.00
39.12
A
O


ATOM
62
N
TRP
A
14
65.106
−20.923
−15.437
1.00
31.26
A
N


ATOM
63
CA
TRP
A
14
66.150
−21.196
−14.425
1.00
34.62
A
C


ATOM
64
CB
TRP
A
14
65.588
−22.154
−13.360
1.00
31.14
A
C


ATOM
65
CG
TRP
A
14
64.834
−23.336
−13.913
1.00
37.51
A
C


ATOM
66
CD1
TRP
A
14
63.461
−23.457
−14.081
1.00
46.88
A
C


ATOM
67
NE1
TRP
A
14
63.143
−24.667
−14.618
1.00
48.46
A
N


ATOM
68
CE2
TRP
A
14
64.251
−25.407
−14.820
1.00
39.74
A
C


ATOM
69
CD2
TRP
A
14
65.385
−24.609
−14.375
1.00
30.24
A
C


ATOM
70
CE3
TRP
A
14
66.651
−25.128
−14.491
1.00
47.63
A
C


ATOM
71
CZ3
TRP
A
14
66.803
−26.425
−15.013
1.00
54.86
A
C


ATOM
72
CH2
TRP
A
14
65.705
−27.181
−15.419
1.00
55.53
A
C


ATOM
73
CZ2
TRP
A
14
64.403
−26.682
−15.326
1.00
52.45
A
C


ATOM
74
C
TRP
A
14
67.500
−21.691
−14.975
1.00
35.77
A
C


ATOM
75
O
TRP
A
14
68.442
−21.945
−14.213
1.00
29.88
A
O


ATOM
76
N
ASP
A
15
67.634
−21.833
−16.294
1.00
31.54
A
N


ATOM
77
CA
ASP
A
15
68.926
−22.238
−16.858
1.00
30.12
A
C


ATOM
78
CB
ASP
A
15
68.886
−22.314
−18.387
1.00
35.22
A
C


ATOM
79
CG
ASP
A
15
68.006
−23.457
−18.916
1.00
35.51
A
C


ATOM
80
OD1
ASP
A
15
67.780
−24.458
−18.212
1.00
39.20
A
O


ATOM
81
OD2
ASP
A
15
67.562
−23.353
−20.077
1.00
46.90
A
O


ATOM
82
C
ASP
A
15
70.003
−21.240
−16.427
1.00
29.97
A
C


ATOM
83
O
ASP
A
15
69.726
−20.035
−16.282
1.00
27.48
A
O


ATOM
84
N
TYR
A
16
71.222
−21.742
−16.220
1.00
24.88
A
N


ATOM
85
CA
TYR
A
16
72.355
−20.905
−15.817
1.00
27.41
A
C


ATOM
86
CB
TYR
A
16
73.587
−21.740
−15.393
1.00
24.08
A
C


ATOM
87
CG
TYR
A
16
73.367
−22.522
−14.085
1.00
21.97
A
C


ATOM
88
CD1
TYR
A
16
73.002
−21.865
−12.899
1.00
20.51
A
C


ATOM
89
CE1
TYR
A
16
72.795
−22.573
−11.715
1.00
17.26
A
C


ATOM
90
CZ
TYR
A
16
72.923
−23.922
−11.715
1.00
18.76
A
C


ATOM
91
OH
TYR
A
16
72.722
−24.624
−10.573
1.00
23.38
A
O


ATOM
92
CE2
TYR
A
16
73.269
−24.611
−12.869
1.00
20.65
A
C


ATOM
93
CD2
TYR
A
16
73.511
−23.906
−14.045
1.00
21.95
A
C


ATOM
94
C
TYR
A
16
72.731
−19.918
−16.906
1.00
29.38
A
C


ATOM
95
O
TYR
A
16
73.185
−18.812
−16.600
1.00
32.24
A
O


ATOM
96
N
ASP
A
17
72.561
−20.310
−18.168
1.00
28.07
A
N


ATOM
97
CA
ASP
A
17
72.813
−19.388
−19.284
1.00
37.53
A
C


ATOM
98
CB
ASP
A
17
72.589
−20.099
−20.623
1.00
33.68
A
C


ATOM
99
CG
ASP
A
17
73.757
−21.003
−21.016
1.00
33.51
A
C


ATOM
100
OD1
ASP
A
17
74.920
−20.713
−20.659
1.00
39.78
A
O


ATOM
101
OD2
ASP
A
17
73.506
−22.002
−21.718
1.00
33.90
A
O


ATOM
102
C
ASP
A
17
71.927
−18.126
−19.150
1.00
38.47
A
C


ATOM
103
O
ASP
A
17
72.400
−17.000
−19.350
1.00
39.61
A
O


ATOM
104
N
TYR
A
18
70.667
−18.356
−18.773
1.00
36.67
A
N


ATOM
105
CA
TYR
A
18
69.679
−17.312
−18.474
1.00
38.99
A
C


ATOM
106
CB
TYR
A
18
68.322
−18.001
−18.304
1.00
57.51
A
C


ATOM
107
CG
TYR
A
18
67.110
−17.380
−18.946
1.00
76.08
A
C


ATOM
108
CD1
TYR
A
18
66.449
−16.309
−18.344
1.00
93.77
A
C


ATOM
109
CE1
TYR
A
18
65.305
−15.764
−18.914
1.00
99.10
A
C


ATOM
110
CZ
TYR
A
18
64.795
−16.315
−20.081
1.00
101.95
A
C


ATOM
111
OH
TYR
A
18
63.667
−15.777
−20.644
1.00
100.31
A
O


ATOM
112
CE2
TYR
A
18
65.417
−17.397
−20.684
1.00
93.17
A
C


ATOM
113
CD2
TYR
A
18
66.560
−17.931
−20.108
1.00
85.18
A
C


ATOM
114
C
TYR
A
18
70.049
−16.539
−17.174
1.00
42.81
A
C


ATOM
115
O
TYR
A
18
70.299
−15.343
−17.213
1.00
34.84
A
O


ATOM
116
N
LEU
A
19
70.122
−17.235
−16.032
1.00
39.87
A
N


ATOM
117
CA
LEU
A
19
70.283
−16.598
−14.704
1.00
38.49
A
C


ATOM
118
CB
LEU
A
19
69.875
−17.580
−13.586
1.00
34.82
A
C


ATOM
119
CG
LEU
A
19
68.416
−18.018
−13.434
1.00
37.78
A
C


ATOM
120
CD1
LEU
A
19
68.275
−19.058
−12.276
1.00
29.08
A
C


ATOM
121
CD2
LEU
A
19
67.489
−16.786
−13.219
1.00
30.96
A
C


ATOM
122
C
LEU
A
19
71.695
−16.041
−14.373
1.00
40.09
A
C


ATOM
123
O
LEU
A
19
71.822
−15.105
−13.588
1.00
36.68
A
O


ATOM
124
N
LEU
A
20
72.748
−16.645
−14.916
1.00
38.96
A
N


ATOM
125
CA
LEU
A
20
74.113
−16.187
−14.645
1.00
43.59
A
C


ATOM
126
CB
LEU
A
20
75.023
−17.350
−14.186
1.00
42.50
A
C


ATOM
127
CG
LEU
A
20
74.766
−18.088
−12.860
1.00
42.59
A
C


ATOM
128
CD1
LEU
A
20
75.842
−19.151
−12.616
1.00
34.09
A
C


ATOM
129
CD2
LEU
A
20
74.697
−17.094
−11.706
1.00
45.62
A
C


ATOM
130
C
LEU
A
20
74.706
−15.498
−15.885
1.00
50.43
A
C


ATOM
131
O
LEU
A
20
75.718
−15.945
−16.436
1.00
43.53
A
O


ATOM
132
N
SER
A
21
74.056
−14.433
−16.339
1.00
66.73
A
N


ATOM
133
CA
SER
A
21
74.699
−13.479
−17.256
1.00
77.64
A
C


ATOM
134
CB
SER
A
21
74.005
−13.478
−18.617
1.00
70.83
A
C


ATOM
135
OG
SER
A
21
74.122
−14.749
−19.235
1.00
68.52
A
O


ATOM
136
C
SER
A
21
74.719
−12.077
−16.645
1.00
88.35
A
C


ATOM
137
O
SER
A
21
75.120
−11.118
−17.305
1.00
98.79
A
O


ATOM
138
N
SER
A
22
74.305
−11.976
−15.379
1.00
97.10
A
N


ATOM
139
CA
SER
A
22
74.314
−10.721
−14.634
1.00
99.45
A
C


ATOM
140
CB
SER
A
22
73.092
−9.875
−14.992
1.00
94.35
A
C


ATOM
141
OG
SER
A
22
72.999
−8.745
−14.147
1.00
89.75
A
O


ATOM
142
C
SER
A
22
74.340
−11.011
−13.134
1.00
93.31
A
C


ATOM
143
O
SER
A
22
75.174
−11.787
−12.661
1.00
86.27
A
O


ATOM
144
N
GLU
A
26
81.547
−9.426
−13.066
1.00
77.98
A
N


ATOM
145
CA
GLU
A
26
81.323
−9.634
−14.494
1.00
81.96
A
C


ATOM
146
CB
GLU
A
26
81.137
−8.290
−15.197
1.00
81.82
A
C


ATOM
147
CG
GLU
A
26
79.899
−7.547
−14.719
1.00
83.63
A
C


ATOM
148
CD
GLU
A
26
79.387
−6.539
−15.730
1.00
86.04
A
C


ATOM
149
OE1
GLU
A
26
80.202
−5.766
−16.271
1.00
81.48
A
O


ATOM
150
OE2
GLU
A
26
78.164
−6.518
−15.982
1.00
99.87
A
O


ATOM
151
C
GLU
A
26
82.403
−10.494
−15.184
1.00
82.65
A
C


ATOM
152
O
GLU
A
26
82.764
−10.263
−16.341
1.00
72.41
A
O


ATOM
153
N
SER
A
27
82.936
−11.463
−14.447
1.00
83.75
A
N


ATOM
154
CA
SER
A
27
83.361
−12.726
−15.039
1.00
81.10
A
C


ATOM
155
CB
SER
A
27
84.878
−12.932
−14.991
1.00
78.85
A
C


ATOM
156
OG
SER
A
27
85.289
−13.594
−13.814
1.00
78.66
A
O


ATOM
157
C
SER
A
27
82.577
−13.797
−14.276
1.00
94.31
A
C


ATOM
158
O
SER
A
27
83.095
−14.861
−13.927
1.00
100.03
A
O


ATOM
159
N
ILE
A
28
81.306
−13.470
−14.017
1.00
92.06
A
N


ATOM
160
CA
ILE
A
28
80.292
−14.426
−13.590
1.00
83.46
A
C


ATOM
161
CB
ILE
A
28
78.923
−13.698
−13.339
1.00
79.85
A
C


ATOM
162
CG1
ILE
A
28
78.999
−12.785
−12.096
1.00
75.89
A
C


ATOM
163
CD1
ILE
A
28
79.113
−13.507
−10.743
1.00
64.21
A
C


ATOM
164
CG2
ILE
A
28
77.773
−14.681
−13.196
1.00
77.42
A
C


ATOM
165
C
ILE
A
28
80.183
−15.522
−14.671
1.00
84.44
A
C


ATOM
166
O
ILE
A
28
79.486
−16.521
−14.489
1.00
75.29
A
O


ATOM
167
N
GLU
A
29
80.872
−15.294
−15.796
1.00
79.42
A
N


ATOM
168
CA
GLU
A
29
81.163
−16.298
−16.833
1.00
69.34
A
C


ATOM
169
CB
GLU
A
29
82.075
−15.677
−17.898
1.00
70.95
A
C


ATOM
170
CG
GLU
A
29
81.339
−14.975
−19.010
1.00
80.65
A
C


ATOM
171
CD
GLU
A
29
80.700
−15.953
−19.977
1.00
78.28
A
C


ATOM
172
OE1
GLU
A
29
81.451
−16.734
−20.604
1.00
63.52
A
O


ATOM
173
OE2
GLU
A
29
79.454
−15.938
−20.109
1.00
67.73
A
O


ATOM
174
C
GLU
A
29
81.848
−17.570
−16.343
1.00
67.94
A
C


ATOM
175
O
GLU
A
29
81.490
−18.677
−16.759
1.00
54.47
A
O


ATOM
176
N
VAL
A
30
82.867
−17.408
−15.503
1.00
66.57
A
N


ATOM
177
CA
VAL
A
30
83.629
−18.554
−15.006
1.00
55.26
A
C


ATOM
178
CB
VAL
A
30
85.025
−18.151
−14.446
1.00
50.13
A
C


ATOM
179
CG1
VAL
A
30
85.940
−19.367
−14.387
1.00
43.43
A
C


ATOM
180
CG2
VAL
A
30
85.669
−17.054
−15.305
1.00
49.92
A
C


ATOM
181
C
VAL
A
30
82.793
−19.297
−13.954
1.00
45.40
A
C


ATOM
182
O
VAL
A
30
82.865
−20.523
−13.870
1.00
43.51
A
O


ATOM
183
N
TYR
A
31
81.998
−18.548
−13.177
1.00
41.39
A
N


ATOM
184
CA
TYR
A
31
80.996
−19.102
−12.240
1.00
43.11
A
C


ATOM
185
CB
TYR
A
31
80.376
−17.939
−11.430
1.00
47.10
A
C


ATOM
186
CG
TYR
A
31
79.407
−18.221
−10.261
1.00
54.66
A
C


ATOM
187
CD1
TYR
A
31
79.865
−18.624
−8.995
1.00
55.24
A
C


ATOM
188
CE1
TYR
A
31
78.948
−18.818
−7.896
1.00
58.05
A
C


ATOM
189
CZ
TYR
A
31
77.565
−18.576
−8.070
1.00
70.02
A
C


ATOM
190
OH
TYR
A
31
76.618
−18.756
−7.021
1.00
31.58
A
O


ATOM
191
CE2
TYR
A
31
77.116
−18.143
−9.324
1.00
70.34
A
C


ATOM
192
CD2
TYR
A
31
78.033
−17.951
−10.394
1.00
69.01
A
C


ATOM
193
C
TYR
A
31
79.915
−19.902
−13.011
1.00
38.65
A
C


ATOM
194
O
TYR
A
31
79.559
−21.013
−12.620
1.00
30.30
A
O


ATOM
195
N
LYS
A
32
79.419
−19.335
−14.115
1.00
37.41
A
N


ATOM
196
CA
LYS
A
32
78.408
−19.989
−14.969
1.00
32.39
A
C


ATOM
197
CB
LYS
A
32
77.920
−19.032
−16.068
1.00
34.41
A
C


ATOM
198
CG
LYS
A
32
77.173
−19.718
−17.212
1.00
40.68
A
C


ATOM
199
CD
LYS
A
32
76.327
−18.748
−18.034
1.00
49.83
A
C


ATOM
200
CE
LYS
A
32
77.138
−17.767
−18.852
1.00
49.40
A
C


ATOM
201
NZ
LYS
A
32
77.502
−18.323
−20.169
1.00
53.46
A
N


ATOM
202
C
LYS
A
32
78.932
−21.292
−15.586
1.00
24.83
A
C


ATOM
203
O
LYS
A
32
78.275
−22.315
−15.500
1.00
25.50
A
O


ATOM
204
N
ASP
A
33
80.109
−21.262
−16.194
1.00
24.37
A
N


ATOM
205
CA
ASP
A
33
80.701
−22.478
−16.752
1.00
25.75
A
C


ATOM
206
CB
ASP
A
33
82.001
−22.192
−17.499
1.00
33.04
A
C


ATOM
207
CG
ASP
A
33
81.767
−21.560
−18.869
1.00
40.42
A
C


ATOM
208
OD1
ASP
A
33
80.634
−21.654
−19.398
1.00
45.69
A
O


ATOM
209
OD2
ASP
A
33
82.729
−20.981
−19.421
1.00
41.92
A
O


ATOM
210
C
ASP
A
33
80.941
−23.532
−15.677
1.00
29.91
A
C


ATOM
211
O
ASP
A
33
80.847
−24.721
−15.956
1.00
22.59
A
O


ATOM
212
N
LYS
A
34
81.229
−23.088
−14.454
1.00
28.83
A
N


ATOM
213
CA
LYS
A
34
81.440
−23.997
−13.335
1.00
27.61
A
C


ATOM
214
CB
LYS
A
34
82.071
−23.229
−12.149
1.00
33.30
A
C


ATOM
215
CG
LYS
A
34
82.285
−23.993
−10.832
1.00
32.01
A
C


ATOM
216
CD
LYS
A
34
83.474
−24.963
−10.867
1.00
49.59
A
C


ATOM
217
CE
LYS
A
34
83.183
−26.189
−11.757
1.00
50.50
A
C


ATOM
218
NZ
LYS
A
34
83.640
−27.463
−11.181
1.00
41.90
A
N


ATOM
219
C
LYS
A
34
80.103
−24.658
−12.968
1.00
22.42
A
C


ATOM
220
O
LYS
A
34
80.017
−25.884
−12.834
1.00
20.81
A
O


ATOM
221
N
ALA
A
35
79.053
−23.862
−12.821
1.00
18.29
A
N


ATOM
222
CA
ALA
A
35
77.732
−24.397
−12.506
1.00
18.56
A
C


ATOM
223
CB
ALA
A
35
76.730
−23.280
−12.355
1.00
23.87
A
C


ATOM
224
C
ALA
A
35
77.252
−25.393
−13.555
1.00
17.36
A
C


ATOM
225
O
ALA
A
35
76.787
−26.498
−13.228
1.00
16.12
A
O


ATOM
226
N
LYS
A
36
77.418
−25.038
−14.821
1.00
15.09
A
N


ATOM
227
CA
LYS
A
36
76.989
−25.916
−15.911
1.00
16.19
A
C


ATOM
228
CB
LYS
A
36
77.195
−25.206
−17.251
1.00
20.04
A
C


ATOM
229
CG
LYS
A
36
76.070
−24.130
−17.446
1.00
20.11
A
C


ATOM
230
CD
LYS
A
36
76.355
−23.138
−18.530
1.00
19.35
A
C


ATOM
231
CE
LYS
A
36
76.571
−23.797
−19.854
1.00
21.87
A
C


ATOM
232
NZ
LYS
A
36
76.850
−22.796
−20.953
1.00
24.73
A
N


ATOM
233
C
LYS
A
36
77.657
−27.290
−15.904
1.00
16.32
A
C


ATOM
234
O
LYS
A
36
77.001
−28.299
−16.145
1.00
17.53
A
O


ATOM
235
N
LYS
A
37
78.959
−27.325
−15.631
1.00
15.35
A
N


ATOM
236
CA
LYS
A
37
79.733
−28.589
−15.646
1.00
15.48
A
C


ATOM
237
CB
LYS
A
37
81.247
−28.252
−15.606
1.00
18.37
A
C


ATOM
238
CG
LYS
A
37
82.147
−29.422
−15.470
1.00
22.75
A
C


ATOM
239
CD
LYS
A
37
83.628
−29.045
−15.576
1.00
26.96
A
C


ATOM
240
CE
LYS
A
37
84.463
−30.314
−15.779
1.00
30.90
A
C


ATOM
241
NZ
LYS
A
37
85.904
−30.113
−15.499
1.00
41.14
A
N


ATOM
242
C
LYS
A
37
79.291
−29.500
−14.476
1.00
14.16
A
C


ATOM
243
O
LYS
A
37
79.087
−30.727
−14.627
1.00
12.16
A
O


ATOM
244
N
LEU
A
38
79.095
−28.888
−13.319
1.00
12.16
A
N


ATOM
245
CA
LEU
A
38
78.557
−29.611
−12.169
1.00
12.46
A
C


ATOM
246
CB
LEU
A
38
78.569
−28.705
−10.943
1.00
11.62
A
C


ATOM
247
CG
LEU
A
38
79.948
−28.214
−10.491
1.00
13.13
A
C


ATOM
248
CD1
LEU
A
38
79.786
−27.177
−9.428
1.00
13.41
A
C


ATOM
249
CD2
LEU
A
38
80.845
−29.365
−10.003
1.00
15.16
A
C


ATOM
250
C
LEU
A
38
77.131
−30.088
−12.431
1.00
12.95
A
C


ATOM
251
O
LEU
A
38
76.788
−31.249
−12.177
1.00
11.60
A
O


ATOM
252
N
GLU
A
39
76.275
−29.193
−12.919
1.00
11.82
A
N


ATOM
253
CA
GLU
A
39
74.891
−29.586
−13.221
1.00
12.20
A
C


ATOM
254
CB
GLU
A
39
74.127
−28.385
−13.767
1.00
16.05
A
C


ATOM
255
CG
GLU
A
39
72.731
−28.656
−14.294
1.00
21.03
A
C


ATOM
256
CD
GLU
A
39
72.130
−27.381
−14.877
1.00
23.31
A
C


ATOM
257
OE1
GLU
A
39
72.820
−26.681
−15.648
1.00
23.15
A
O


ATOM
258
OE2
GLU
A
39
70.986
−27.071
−14.533
1.00
22.30
A
O


ATOM
259
C
GLU
A
39
74.816
−30.756
−14.184
1.00
10.69
A
C


ATOM
260
O
GLU
A
39
74.012
−31.673
−14.001
1.00
11.30
A
O


ATOM
261
N
ALA
A
40
75.685
−30.741
−15.197
1.00
11.54
A
N


ATOM
262
CA
ALA
A
40
75.767
−31.785
−16.201
1.00
10.76
A
C


ATOM
263
CB
ALA
A
40
76.918
−31.447
−17.199
1.00
12.41
A
C


ATOM
264
C
ALA
A
40
76.041
−33.164
−15.575
1.00
14.03
A
C


ATOM
265
O
ALA
A
40
75.409
−34.180
−15.911
1.00
10.38
A
O


ATOM
266
N
GLU
A
41
77.024
−33.200
−14.687
1.00
16.09
A
N


ATOM
267
CA
GLU
A
41
77.390
−34.441
−14.017
1.00
13.91
A
C


ATOM
268
CB
GLU
A
41
78.665
−34.237
−13.212
1.00
19.27
A
C


ATOM
269
CG
GLU
A
41
79.138
−35.483
−12.508
1.00
25.02
A
C


ATOM
270
CD
GLU
A
41
80.607
−35.425
−12.091
1.00
28.02
A
C


ATOM
271
OE1
GLU
A
41
81.375
−34.548
−12.576
1.00
24.47
A
O


ATOM
272
OE2
GLU
A
41
80.970
−36.273
−11.257
1.00
29.52
A
O


ATOM
273
C
GLU
A
41
76.272
−34.965
−13.108
1.00
12.87
A
C


ATOM
274
O
GLU
A
41
76.090
−36.165
−13.024
1.00
13.64
A
O


ATOM
275
N
VAL
A
42
75.532
−34.091
−12.434
1.00
12.85
A
N


ATOM
276
CA
VAL
A
42
74.421
−34.563
−11.586
1.00
14.03
A
C


ATOM
277
CB
VAL
A
42
73.924
−33.454
−10.695
1.00
14.90
A
C


ATOM
278
CG1
VAL
A
42
72.584
−33.857
−10.061
1.00
13.47
A
C


ATOM
279
CG2
VAL
A
42
74.995
−33.110
−9.638
1.00
12.19
A
C


ATOM
280
C
VAL
A
42
73.276
−35.141
−12.475
1.00
15.79
A
C


ATOM
281
O
VAL
A
42
72.718
−36.203
−12.205
1.00
14.89
A
O


ATOM
282
N
ARG
A
43
73.021
−34.478
−13.604
1.00
16.80
A
N


ATOM
283
CA
ARG
A
43
72.115
−35.001
−14.629
1.00
15.60
A
C


ATOM
284
CB
ARG
A
43
72.092
−34.051
−15.853
1.00
15.25
A
C


ATOM
285
CG
ARG
A
43
71.211
−34.540
−17.003
1.00
16.80
A
C


ATOM
286
CD
ARG
A
43
71.932
−35.504
−17.942
1.00
16.08
A
C


ATOM
287
NE
ARG
A
43
71.013
−35.995
−18.967
1.00
19.00
A
N


ATOM
288
CZ
ARG
A
43
71.278
−36.953
−19.843
1.00
21.10
A
C


ATOM
289
NH1
ARG
A
43
72.472
−37.567
−19.874
1.00
16.27
A
N


ATOM
290
NH2
ARG
A
43
70.323
−37.315
−20.694
1.00
19.98
A
N


ATOM
291
C
ARG
A
43
72.536
−36.396
−15.060
1.00
13.27
A
C


ATOM
292
O
ARG
A
43
71.722
−37.295
−15.093
1.00
12.89
A
O


ATOM
293
N
ARG
A
44
73.819
−36.583
−15.386
1.00
12.23
A
N


ATOM
294
CA
ARG
A
44
74.287
−37.877
−15.802
1.00
13.34
A
C


ATOM
295
CB
ARG
A
44
75.778
−37.854
−16.129
1.00
13.24
A
C


ATOM
296
CG
ARG
A
44
76.300
−39.154
−16.657
1.00
14.52
A
C


ATOM
297
CD
ARG
A
44
77.795
−39.105
−16.917
1.00
16.25
A
C


ATOM
298
NE
ARG
A
44
78.513
−38.905
−15.676
1.00
15.34
A
N


ATOM
299
CZ
ARG
A
44
79.791
−38.554
−15.561
1.00
22.40
A
C


ATOM
300
NH1
ARG
A
44
80.581
−38.337
−16.624
1.00
18.24
A
N


ATOM
301
NH2
ARG
A
44
80.285
−38.413
−14.338
1.00
26.00
A
N


ATOM
302
C
ARG
A
44
74.029
−38.934
−14.726
1.00
15.66
A
C


ATOM
303
O
ARG
A
44
73.596
−40.025
−15.046
1.00
14.32
A
O


ATOM
304
N
GLU
A
45
74.309
−38.612
−13.464
1.00
16.48
A
N


ATOM
305
CA
GLU
A
45
74.179
−39.621
−12.394
1.00
15.18
A
C


ATOM
306
CB
GLU
A
45
74.884
−39.173
−11.117
1.00
17.44
A
C


ATOM
307
CG
GLU
A
45
76.398
−39.065
−11.261
1.00
23.45
A
C


ATOM
308
CD
GLU
A
45
77.009
−40.405
−11.686
1.00
30.59
A
C


ATOM
309
OE1
GLU
A
45
76.904
−41.378
−10.908
1.00
41.70
A
O


ATOM
310
OE2
GLU
A
45
77.554
−40.495
−12.820
1.00
34.07
A
O


ATOM
311
C
GLU
A
45
72.706
−39.958
−12.106
1.00
14.08
A
C


ATOM
312
O
GLU
A
45
72.401
−41.076
−11.798
1.00
12.78
A
O


ATOM
313
N
ILE
A
46
71.799
−39.015
−12.285
1.00
15.21
A
N


ATOM
314
CA
ILE
A
46
70.360
−39.321
−12.129
1.00
14.92
A
C


ATOM
315
CB
ILE
A
46
69.546
−38.073
−11.941
1.00
13.75
A
C


ATOM
316
CG1
ILE
A
46
69.968
−37.340
−10.668
1.00
13.13
A
C


ATOM
317
CD1
ILE
A
46
69.335
−35.917
−10.521
1.00
13.11
A
C


ATOM
318
CG2
ILE
A
46
68.010
−38.395
−11.900
1.00
15.50
A
C


ATOM
319
C
ILE
A
46
69.857
−40.187
−13.291
1.00
18.05
A
C


ATOM
320
O
ILE
A
46
69.013
−41.083
−13.103
1.00
18.68
A
O


ATOM
321
N
ASN
A
47
70.398
−39.976
−14.487
1.00
18.26
A
N


ATOM
322
CA
ASN
A
47
69.952
−40.743
−15.668
1.00
16.25
A
C


ATOM
323
CB
ASN
A
47
70.181
−39.936
−16.926
1.00
16.08
A
C


ATOM
324
CG
ASN
A
47
69.170
−38.835
−17.085
1.00
22.48
A
C


ATOM
325
OD1
ASN
A
47
68.166
−39.047
−17.712
1.00
21.93
A
O


ATOM
326
ND2
ASN
A
47
69.411
−37.668
−16.490
1.00
18.56
A
N


ATOM
327
C
ASN
A
47
70.585
−42.115
−15.828
1.00
18.89
A
C


ATOM
328
O
ASN
A
47
70.184
−42.892
−16.689
1.00
21.16
A
O


ATOM
329
N
ASN
A
48
71.615
−42.392
−15.040
1.00
20.07
A
N


ATOM
330
CA
ASN
A
48
72.411
−43.614
−15.177
1.00
21.07
A
C


ATOM
331
CB
ASN
A
48
73.524
−43.561
−14.128
1.00
22.12
A
C


ATOM
332
CG
ASN
A
48
74.333
−44.833
−14.027
1.00
24.42
A
C


ATOM
333
OD1
ASN
A
48
74.362
−45.670
−14.931
1.00
22.04
A
O


ATOM
334
ND2
ASN
A
48
75.046
−44.955
−12.920
1.00
22.68
A
N


ATOM
335
C
ASN
A
48
71.551
−44.840
−14.953
1.00
22.60
A
C


ATOM
336
O
ASN
A
48
71.037
−45.017
−13.878
1.00
24.75
A
O


ATOM
337
N
GLU
A
49
71.404
−45.677
−15.965
1.00
25.27
A
N


ATOM
338
CA
GLU
A
49
70.534
−46.838
−15.873
1.00
31.45
A
C


ATOM
339
CB
GLU
A
49
69.971
−47.183
−17.248
1.00
33.69
A
C


ATOM
340
CG
GLU
A
49
69.010
−46.083
−17.720
1.00
43.39
A
C


ATOM
341
CD
GLU
A
49
68.043
−46.506
−18.832
1.00
49.09
A
C


ATOM
342
OE1
GLU
A
49
68.038
−47.692
−19.225
1.00
45.42
A
O


ATOM
343
OE2
GLU
A
49
67.282
−45.626
−19.308
1.00
40.84
A
O


ATOM
344
C
GLU
A
49
71.177
−48.050
−15.241
1.00
34.38
A
C


ATOM
345
O
GLU
A
49
70.520
−49.055
−15.058
1.00
37.08
A
O


ATOM
346
N
LYS
A
50
72.455
−47.958
−14.906
1.00
31.70
A
N


ATOM
347
CA
LYS
A
50
73.159
−49.048
−14.255
1.00
36.67
A
C


ATOM
348
CB
LYS
A
50
74.573
−49.191
−14.827
1.00
40.64
A
C


ATOM
349
CG
LYS
A
50
74.689
−49.329
−16.371
1.00
51.97
A
C


ATOM
350
CD
LYS
A
50
75.923
−48.546
−16.896
1.00
56.91
A
C


ATOM
351
CE
LYS
A
50
76.546
−49.104
−18.184
1.00
54.15
A
C


ATOM
352
NZ
LYS
A
50
75.666
−48.933
−19.373
1.00
52.48
A
N


ATOM
353
C
LYS
A
50
73.275
−48.787
−12.758
1.00
33.26
A
C


ATOM
354
O
LYS
A
50
73.835
−49.595
−12.057
1.00
33.42
A
O


ATOM
355
N
ALA
A
51
72.780
−47.654
−12.270
1.00
30.39
A
N


ATOM
356
CA
ALA
A
51
72.905
−47.331
−10.843
1.00
30.68
A
C


ATOM
357
CB
ALA
A
51
72.301
−45.928
−10.554
1.00
29.95
A
C


ATOM
358
C
ALA
A
51
72.173
−48.388
−10.015
1.00
26.77
A
C


ATOM
359
O
ALA
A
51
71.125
−48.866
−10.439
1.00
23.61
A
O


ATOM
360
N
GLU
A
52
72.720
−48.742
−8.851
1.00
25.28
A
N


ATOM
361
CA
GLU
A
52
71.998
−49.553
−7.854
1.00
27.96
A
C


ATOM
362
CB
GLU
A
52
72.881
−49.902
−6.643
1.00
31.71
A
C


ATOM
363
CG
GLU
A
52
74.162
−50.669
−6.959
1.00
49.71
A
C


ATOM
364
CD
GLU
A
52
73.925
−52.024
−7.623
1.00
61.36
A
C


ATOM
365
OE1
GLU
A
52
72.763
−52.463
−7.743
1.00
70.03
A
O


ATOM
366
OE2
GLU
A
52
74.920
−52.659
−8.033
1.00
80.38
A
O


ATOM
367
C
GLU
A
52
70.793
−48.786
−7.336
1.00
26.09
A
C


ATOM
368
O
GLU
A
52
70.879
−47.583
−7.079
1.00
24.96
A
O


ATOM
369
N
PHE
A
53
69.682
−49.490
−7.160
1.00
26.53
A
N


ATOM
370
CA
PHE
A
53
68.388
−48.872
−6.808
1.00
35.85
A
C


ATOM
371
CB
PHE
A
53
67.340
−49.942
−6.445
1.00
49.50
A
C


ATOM
372
CG
PHE
A
53
66.862
−50.770
−7.617
1.00
61.54
A
C


ATOM
373
CD1
PHE
A
53
66.522
−50.159
−8.837
1.00
61.58
A
C


ATOM
374
CE1
PHE
A
53
66.072
−50.919
−9.922
1.00
53.87
A
C


ATOM
375
CZ
PHE
A
53
65.942
−52.308
−9.794
1.00
69.22
A
C


ATOM
376
CE2
PHE
A
53
66.265
−52.937
−8.574
1.00
66.79
A
C


ATOM
377
CD2
PHE
A
53
66.718
−52.163
−7.494
1.00
69.27
A
C


ATOM
378
C
PHE
A
53
68.495
−47.922
−5.634
1.00
27.28
A
C


ATOM
379
O
PHE
A
53
68.084
−46.754
−5.726
1.00
22.90
A
O


ATOM
380
N
LEU
A
54
69.065
−48.423
−4.544
1.00
24.77
A
N


ATOM
381
CA
LEU
A
54
69.123
−47.682
−3.302
1.00
28.10
A
C


ATOM
382
CB
LEU
A
54
69.596
−48.572
−2.154
1.00
34.39
A
C


ATOM
383
CG
LEU
A
54
68.736
−48.556
−0.887
1.00
49.21
A
C


ATOM
384
CD1
LEU
A
54
68.322
−47.145
−0.451
1.00
51.57
A
C


ATOM
385
CD2
LEU
A
54
67.515
−49.439
−1.132
1.00
50.67
A
C


ATOM
386
C
LEU
A
54
70.013
−46.443
−3.396
1.00
27.68
A
C


ATOM
387
O
LEU
A
54
69.681
−45.416
−2.834
1.00
23.39
A
O


ATOM
388
N
THR
A
55
71.142
−46.549
−4.101
1.00
23.55
A
N


ATOM
389
CA
THR
A
55
72.040
−45.423
−4.318
1.00
21.92
A
C


ATOM
390
CB
THR
A
55
73.281
−45.871
−5.101
1.00
23.88
A
C


ATOM
391
OG1
THR
A
55
73.801
−47.016
−4.452
1.00
25.50
A
O


ATOM
392
CG2
THR
A
55
74.332
−44.782
−5.130
1.00
23.93
A
C


ATOM
393
C
THR
A
55
71.343
−44.343
−5.108
1.00
17.55
A
C


ATOM
394
O
THR
A
55
71.434
−43.173
−4.792
1.00
18.91
A
O


ATOM
395
N
LEU
A
56
70.607
−44.748
−6.113
1.00
18.24
A
N


ATOM
396
CA
LEU
A
56
69.862
−43.800
−6.928
1.00
20.04
A
C


ATOM
397
CB
LEU
A
56
69.227
−44.539
−8.105
1.00
22.20
A
C


ATOM
398
CG
LEU
A
56
68.464
−43.635
−9.061
1.00
26.34
A
C


ATOM
399
CD1
LEU
A
56
69.434
−42.666
−9.723
1.00
31.96
A
C


ATOM
400
CD2
LEU
A
56
67.690
−44.445
−10.097
1.00
33.31
A
C


ATOM
401
C
LEU
A
56
68.777
−43.049
−6.149
1.00
19.87
A
C


ATOM
402
O
LEU
A
56
68.634
−41.841
−6.283
1.00
17.72
A
O


ATOM
403
N
LEU
A
57
68.017
−43.771
−5.330
1.00
18.78
A
N


ATOM
404
CA
LEU
A
57
66.988
−43.158
−4.532
1.00
17.43
A
C


ATOM
405
CB
LEU
A
57
66.169
−44.211
−3.795
1.00
17.26
A
C


ATOM
406
CG
LEU
A
57
65.336
−45.160
−4.639
1.00
18.53
A
C


ATOM
407
CD1
LEU
A
57
64.868
−46.280
−3.736
1.00
17.73
A
C


ATOM
408
CD2
LEU
A
57
64.168
−44.475
−5.338
1.00
17.09
A
C


ATOM
409
C
LEU
A
57
67.585
−42.177
−3.549
1.00
16.16
A
C


ATOM
410
O
LEU
A
57
67.023
−41.110
−3.319
1.00
14.89
A
O


ATOM
411
N
GLU
A
58
68.693
−42.559
−2.927
1.00
16.26
A
N


ATOM
412
CA
GLU
A
58
69.381
−41.681
−2.008
1.00
17.82
A
C


ATOM
413
CB
GLU
A
58
70.460
−42.434
−1.225
1.00
25.14
A
C


ATOM
414
CG
GLU
A
58
69.819
−43.406
−0.193
1.00
35.32
A
C


ATOM
415
CD
GLU
A
58
70.827
−44.166
0.686
1.00
48.33
A
C


ATOM
416
OE1
GLU
A
58
72.054
−44.054
0.456
1.00
40.98
A
O


ATOM
417
OE2
GLU
A
58
70.376
−44.885
1.608
1.00
38.51
A
O


ATOM
418
C
GLU
A
58
69.973
−40.453
−2.712
1.00
17.55
A
C


ATOM
419
O
GLU
A
58
69.926
−39.353
−2.157
1.00
14.34
A
O


ATOM
420
N
LEU
A
59
70.524
−40.624
−3.921
1.00
14.91
A
N


ATOM
421
CA
LEU
A
59
70.938
−39.466
−4.720
1.00
14.26
A
C


ATOM
422
CB
LEU
A
59
71.528
−39.906
−6.055
1.00
16.83
A
C


ATOM
423
CG
LEU
A
59
71.832
−38.771
−7.070
1.00
17.41
A
C


ATOM
424
CD1
LEU
A
59
72.898
−37.834
−6.529
1.00
14.87
A
C


ATOM
425
CD2
LEU
A
59
72.258
−39.366
−8.400
1.00
17.18
A
C


ATOM
426
C
LEU
A
59
69.780
−38.486
−4.976
1.00
13.47
A
C


ATOM
427
O
LEU
A
59
69.901
−37.259
−4.762
1.00
18.07
A
O


ATOM
428
N
ILE
A
60
68.654
−39.009
−5.456
1.00
14.22
A
N


ATOM
429
CA
ILE
A
60
67.510
−38.174
−5.735
1.00
15.30
A
C


ATOM
430
CB
ILE
A
60
66.311
−39.013
−6.215
1.00
15.27
A
C


ATOM
431
CG1
ILE
A
60
66.569
−39.514
−7.634
1.00
18.44
A
C


ATOM
432
CD1
ILE
A
60
65.650
−40.585
−8.070
1.00
18.66
A
C


ATOM
433
CG2
ILE
A
60
65.001
−38.220
−6.118
1.00
13.95
A
C


ATOM
434
C
ILE
A
60
67.108
−37.415
−4.450
1.00
16.70
A
C


ATOM
435
O
ILE
A
60
66.807
−36.196
−4.457
1.00
16.26
A
O


ATOM
436
N
ASP
A
61
67.064
−38.145
−3.358
1.00
15.80
A
N


ATOM
437
CA
ASP
A
61
66.694
−37.530
−2.066
1.00
18.86
A
C


ATOM
438
CB
ASP
A
61
66.698
−38.587
−0.971
1.00
19.67
A
C


ATOM
439
CG
ASP
A
61
65.989
−38.130
0.318
1.00
26.40
A
C


ATOM
440
OD1
ASP
A
61
65.096
−37.229
0.280
1.00
21.55
A
O


ATOM
441
OD2
ASP
A
61
66.330
−38.722
1.376
1.00
23.18
A
O


ATOM
442
C
ASP
A
61
67.630
−36.378
−1.688
1.00
16.98
A
C


ATOM
443
O
ASP
A
61
67.185
−35.298
−1.260
1.00
18.46
A
O


ATOM
444
N
ASN
A
62
68.939
−36.607
−1.826
1.00
19.40
A
N


ATOM
445
CA
ASN
A
62
69.916
−35.574
−1.529
1.00
15.80
A
C


ATOM
446
CB
ASN
A
62
71.364
−36.120
−1.610
1.00
17.49
A
C


ATOM
447
CG
ASN
A
62
71.717
−37.018
−0.442
1.00
20.29
A
C


ATOM
448
OD1
ASN
A
62
71.275
−36.780
0.657
1.00
23.89
A
O


ATOM
449
ND2
ASN
A
62
72.522
−38.057
−0.689
1.00
18.30
A
N


ATOM
450
C
ASN
A
62
69.761
−34.380
−2.472
1.00
16.16
A
C


ATOM
451
O
ASN
A
62
69.742
−33.232
−2.018
1.00
15.22
A
O


ATOM
452
N
VAL
A
63
69.630
−34.648
−3.772
1.00
15.09
A
N


ATOM
453
CA
VAL
A
63
69.406
−33.553
−4.758
1.00
14.55
A
C


ATOM
454
CB
VAL
A
63
69.167
−34.127
−6.161
1.00
15.48
A
C


ATOM
455
CG1
VAL
A
63
68.662
−33.057
−7.117
1.00
15.19
A
C


ATOM
456
CG2
VAL
A
63
70.487
−34.805
−6.685
1.00
13.61
A
C


ATOM
457
C
VAL
A
63
68.235
−32.660
−4.332
1.00
15.51
A
C


ATOM
458
O
VAL
A
63
68.340
−31.431
−4.333
1.00
18.72
A
O


ATOM
459
N
GLN
A
64
67.133
−33.285
−3.920
1.00
15.10
A
N


ATOM
460
CA
GLN
A
64
65.957
−32.562
−3.455
1.00
15.57
A
C


ATOM
461
CB
GLN
A
64
64.747
−33.526
−3.344
1.00
17.68
A
C


ATOM
462
CG
GLN
A
64
64.318
−34.015
−4.711
1.00
17.50
A
C


ATOM
463
CD
GLN
A
64
62.971
−34.726
−4.748
1.00
20.03
A
C


ATOM
464
OE1
GLN
A
64
62.382
−34.802
−5.810
1.00
19.14
A
O


ATOM
465
NE2
GLN
A
64
62.489
−35.252
−3.609
1.00
14.20
A
N


ATOM
466
C
GLN
A
64
66.175
−31.788
−2.143
1.00
14.86
A
C


ATOM
467
O
GLN
A
64
65.873
−30.605
−2.042
1.00
15.46
A
O


ATOM
468
N
ARG
A
65
66.705
−32.453
−1.137
1.00
14.05
A
N


ATOM
469
CA
ARG
A
65
66.904
−31.822
0.161
1.00
15.82
A
C


ATOM
470
CB
ARG
A
65
67.355
−32.847
1.196
1.00
16.25
A
C


ATOM
471
CG
ARG
A
65
66.247
−33.875
1.445
1.00
19.90
A
C


ATOM
472
CD
ARG
A
65
66.533
−34.782
2.609
1.00
20.04
A
C


ATOM
473
NE
ARG
A
65
66.639
−33.958
3.802
1.00
27.75
A
N


ATOM
474
CZ
ARG
A
65
66.875
−34.416
5.027
1.00
47.19
A
C


ATOM
475
NH1
ARG
A
65
67.009
−35.722
5.231
1.00
44.64
A
N


ATOM
476
NH2
ARG
A
65
66.958
−33.559
6.054
1.00
35.23
A
N


ATOM
477
C
ARG
A
65
67.873
−30.645
0.090
1.00
15.18
A
C


ATOM
478
O
ARG
A
65
67.618
−29.607
0.698
1.00
18.75
A
O


ATOM
479
N
LEU
A
66
68.909
−30.769
−0.734
1.00
14.43
A
N


ATOM
480
CA
LEU
A
66
69.898
−29.699
−0.937
1.00
15.85
A
C


ATOM
481
CB
LEU
A
66
71.120
−30.253
−1.690
1.00
14.34
A
C


ATOM
482
CG
LEU
A
66
71.936
−31.227
−0.849
1.00
16.97
A
C


ATOM
483
CD1
LEU
A
66
72.981
−31.901
−1.711
1.00
21.09
A
C


ATOM
484
CD2
LEU
A
66
72.608
−30.479
0.299
1.00
18.12
A
C


ATOM
485
C
LEU
A
66
69.351
−28.524
−1.693
1.00
18.23
A
C


ATOM
486
O
LEU
A
66
70.037
−27.523
−1.853
1.00
18.00
A
O


ATOM
487
N
GLY
A
67
68.139
−28.661
−2.215
1.00
17.75
A
N


ATOM
488
CA
GLY
A
67
67.432
−27.546
−2.817
1.00
15.94
A
C


ATOM
489
C
GLY
A
67
67.409
−27.543
−4.328
1.00
17.22
A
C


ATOM
490
O
GLY
A
67
66.988
−26.556
−4.918
1.00
19.11
A
O


ATOM
491
N
LEU
A
68
67.851
−28.634
−4.963
1.00
17.17
A
N


ATOM
492
CA
LEU
A
68
68.050
−28.660
−6.409
1.00
15.10
A
C


ATOM
493
CB
LEU
A
68
69.433
−29.273
−6.746
1.00
14.65
A
C


ATOM
494
CG
LEU
A
68
70.654
−28.481
−6.265
1.00
17.60
A
C


ATOM
495
CD1
LEU
A
68
71.899
−29.411
−6.237
1.00
14.01
A
C


ATOM
496
CD2
LEU
A
68
70.888
−27.248
−7.175
1.00
14.30
A
C


ATOM
497
C
LEU
A
68
66.955
−29.409
−7.148
1.00
17.27
A
C


ATOM
498
O
LEU
A
68
67.045
−29.590
−8.371
1.00
16.93
A
O


ATOM
499
N
GLY
A
69
65.902
−29.851
−6.447
1.00
19.21
A
N


ATOM
500
CA
GLY
A
69
64.864
−30.621
−7.100
1.00
21.34
A
C


ATOM
501
C
GLY
A
69
64.274
−29.941
−8.319
1.00
22.17
A
C


ATOM
502
O
GLY
A
69
64.052
−30.579
−9.363
1.00
22.04
A
O


ATOM
503
N
TYR
A
70
64.083
−28.631
−8.205
1.00
20.57
A
N


ATOM
504
CA
TYR
A
70
63.435
−27.864
−9.258
1.00
24.61
A
C


ATOM
505
CB
TYR
A
70
63.178
−26.404
−8.827
1.00
22.29
A
C


ATOM
506
CG
TYR
A
70
64.422
−25.554
−8.871
1.00
19.75
A
C


ATOM
507
CD1
TYR
A
70
65.402
−25.637
−7.864
1.00
18.08
A
C


ATOM
508
CE1
TYR
A
70
66.566
−24.891
−7.942
1.00
18.63
A
C


ATOM
509
CZ
TYR
A
70
66.746
−24.025
−9.014
1.00
19.34
A
C


ATOM
510
OH
TYR
A
70
67.887
−23.263
−9.175
1.00
16.26
A
O


ATOM
511
CE2
TYR
A
70
65.789
−23.954
−10.015
1.00
20.55
A
C


ATOM
512
CD2
TYR
A
70
64.650
−24.705
−9.938
1.00
19.66
A
C


ATOM
513
C
TYR
A
70
64.228
−27.898
−10.550
1.00
25.17
A
C


ATOM
514
O
TYR
A
70
63.661
−27.687
−11.608
1.00
22.72
A
O


ATOM
515
N
ARG
A
71
65.539
−28.130
−10.477
1.00
18.92
A
N


ATOM
516
CA
ARG
A
71
66.392
−28.102
−11.666
1.00
18.69
A
C


ATOM
517
CB
ARG
A
71
67.809
−27.634
−11.190
1.00
22.29
A
C


ATOM
518
CG
ARG
A
71
68.902
−27.719
−12.174
1.00
33.98
A
C


ATOM
519
CD
ARG
A
71
70.148
−26.850
−11.726
1.00
34.42
A
C


ATOM
520
NE
ARG
A
71
69.737
−25.505
−11.451
1.00
22.66
A
N


ATOM
521
CZ
ARG
A
71
69.603
−24.529
−12.349
1.00
20.28
A
C


ATOM
522
NH1
ARG
A
71
69.910
−24.686
−13.614
1.00
25.84
A
N


ATOM
523
NH2
ARG
A
71
69.164
−23.355
−11.943
1.00
18.66
A
N


ATOM
524
C
ARG
A
71
66.450
−29.454
−12.393
1.00
19.16
A
C


ATOM
525
O
ARG
A
71
66.865
−29.549
−13.549
1.00
16.93
A
O


ATOM
526
N
PHE
A
72
66.040
−30.510
−11.710
1.00
19.98
A
N


ATOM
527
CA
PHE
A
72
66.189
−31.877
−12.212
1.00
18.22
A
C


ATOM
528
CB
PHE
A
72
67.167
−32.615
−11.297
1.00
18.72
A
C


ATOM
529
CG
PHE
A
72
68.556
−32.062
−11.352
1.00
15.33
A
C


ATOM
530
CD1
PHE
A
72
69.334
−32.258
−12.476
1.00
17.79
A
C


ATOM
531
CE1
PHE
A
72
70.602
−31.747
−12.543
1.00
16.36
A
C


ATOM
532
CZ
PHE
A
72
71.113
−30.998
−11.499
1.00
16.15
A
C


ATOM
533
CE2
PHE
A
72
70.350
−30.793
−10.385
1.00
14.98
A
C


ATOM
534
CD2
PHE
A
72
69.068
−31.326
−10.320
1.00
14.87
A
C


ATOM
535
C
PHE
A
72
64.925
−32.662
−12.295
1.00
22.11
A
C


ATOM
536
O
PHE
A
72
64.968
−33.900
−12.347
1.00
20.25
A
O


ATOM
537
N
GLU
A
73
63.787
−31.965
−12.329
1.00
25.45
A
N


ATOM
538
CA
GLU
A
73
62.514
−32.638
−12.154
1.00
27.06
A
C


ATOM
539
CB
GLU
A
73
61.344
−31.649
−12.123
1.00
29.03
A
C


ATOM
540
CG
GLU
A
73
60.061
−32.289
−11.662
1.00
36.46
A
C


ATOM
541
CD
GLU
A
73
58.884
−31.321
−11.634
1.00
54.40
A
C


ATOM
542
OE1
GLU
A
73
59.116
−30.091
−11.532
1.00
48.00
A
O


ATOM
543
OE2
GLU
A
73
57.728
−31.805
−11.711
1.00
42.07
A
O


ATOM
544
C
GLU
A
73
62.282
−33.701
−13.208
1.00
23.81
A
C


ATOM
545
O
GLU
A
73
61.900
−34.844
−12.873
1.00
22.20
A
O


ATOM
546
N
SER
A
74
62.519
−33.358
−14.467
1.00
22.35
A
N


ATOM
547
CA
ASER
A
74
62.290
−34.318
−15.548
0.50
24.87
A
C


ATOM
548
CA
BSER
A
74
62.341
−34.306
−15.575
0.50
24.62
A
C


ATOM
549
CB
ASER
A
74
62.379
−33.663
−16.937
0.50
27.13
A
C


ATOM
550
CB
BSER
A
74
62.668
−33.651
−16.909
0.50
27.23
A
C


ATOM
551
OG
ASER
A
74
63.690
−33.692
−17.476
0.50
32.13
A
O


ATOM
552
OG
BSER
A
74
61.765
−32.616
−17.190
0.50
30.66
A
O


ATOM
553
C
SER
A
74
63.227
−35.526
−15.419
1.00
23.45
A
C


ATOM
554
O
SER
A
74
62.809
−36.654
−15.663
1.00
23.95
A
O


ATOM
555
N
ASP
A
75
64.477
−35.303
−15.017
1.00
19.65
A
N


ATOM
556
CA
ASP
A
75
65.427
−36.412
−14.826
1.00
20.84
A
C


ATOM
557
CB
ASP
A
75
66.835
−35.869
−14.535
1.00
22.51
A
C


ATOM
558
CG
ASP
A
75
67.319
−34.911
−15.609
1.00
29.28
A
C


ATOM
559
OD1
ASP
A
75
67.561
−35.386
−16.734
1.00
26.21
A
O


ATOM
560
OD2
ASP
A
75
67.438
−33.696
−15.342
1.00
35.89
A
O


ATOM
561
C
ASP
A
75
64.972
−37.340
−13.661
1.00
16.01
A
C


ATOM
562
O
ASP
A
75
65.069
−38.559
−13.741
1.00
14.80
A
O


ATOM
563
N
ILE
A
76
64.549
−36.729
−12.569
1.00
13.98
A
N


ATOM
564
CA
ILE
A
76
64.037
−37.461
−11.393
1.00
14.87
A
C


ATOM
565
CB
ILE
A
76
63.704
−36.489
−10.249
1.00
14.39
A
C


ATOM
566
CG1
ILE
A
76
65.026
−35.919
−9.708
1.00
15.22
A
C


ATOM
567
CD1
ILE
A
76
64.910
−34.789
−8.807
1.00
15.21
A
C


ATOM
568
CG2
ILE
A
76
62.950
−37.214
−9.100
1.00
12.91
A
C


ATOM
569
C
ILE
A
76
62.811
−38.292
−11.791
1.00
15.71
A
C


ATOM
570
O
ILE
A
76
62.735
−39.470
−11.500
1.00
17.94
A
O


ATOM
571
N
ARG
A
77
61.870
−37.684
−12.470
1.00
18.65
A
N


ATOM
572
CA
AARG
A
77
60.670
−38.385
−12.938
0.50
19.96
A
C


ATOM
573
CA
BARG
A
77
60.672
−38.409
−12.891
0.50
21.56
A
C


ATOM
574
CB
AARG
A
77
59.818
−37.453
−13.790
0.50
22.64
A
C


ATOM
575
CB
BARG
A
77
59.669
−37.479
−13.580
0.50
26.61
A
C


ATOM
576
CG
AARG
A
77
59.104
−36.376
−13.062
0.50
22.43
A
C


ATOM
577
CG
BARG
A
77
58.541
−37.048
−12.690
0.50
33.00
A
C


ATOM
578
CD
AARG
A
77
58.085
−35.708
−14.032
0.50
24.82
A
C


ATOM
579
CD
BARG
A
77
57.233
−36.819
−13.483
0.50
41.27
A
C


ATOM
580
NE
AARG
A
77
57.084
−36.686
−14.465
0.50
27.82
A
N


ATOM
581
NE
BARG
A
77
57.430
−36.132
−14.766
0.50
38.02
A
N


ATOM
582
CZ
AARG
A
77
56.062
−37.076
−13.710
0.50
32.47
A
C


ATOM
583
CZ
BARG
A
77
57.743
−34.845
−14.906
0.50
38.80
A
C


ATOM
584
NH1
AARG
A
77
55.895
−36.544
−12.499
0.50
28.27
A
N


ATOM
585
NH1
BARG
A
77
57.920
−34.070
−13.842
0.50
36.68
A
N


ATOM
586
NH2
AARG
A
77
55.203
−37.976
−14.169
0.50
30.50
A
N


ATOM
587
NH2
BARG
A
77
57.892
−34.330
−16.121
0.50
39.56
A
N


ATOM
588
C
ARG
A
77
61.001
−39.588
−13.783
1.00
21.28
A
C


ATOM
589
O
ARG
A
77
60.401
−40.664
−13.637
1.00
23.22
A
O


ATOM
590
N
GLY
A
78
61.944
−39.398
−14.706
1.00
22.93
A
N


ATOM
591
CA
GLY
A
78
62.399
−40.504
−15.558
1.00
21.83
A
C


ATOM
592
C
GLY
A
78
63.046
−41.619
−14.781
1.00
20.05
A
C


ATOM
593
O
GLY
A
78
62.836
−42.793
−15.072
1.00
19.81
A
O


ATOM
594
N
ALA
A
79
63.850
−41.272
−13.778
1.00
19.76
A
N


ATOM
595
CA
ALA
A
79
64.509
−42.292
−12.967
1.00
19.48
A
C


ATOM
596
CB
ALA
A
79
65.542
−41.631
−12.006
1.00
16.46
A
C


ATOM
597
C
ALA
A
79
63.466
−43.102
−12.180
1.00
17.42
A
C


ATOM
598
O
ALA
A
79
63.564
−44.316
−12.041
1.00
18.48
A
O


ATOM
599
N
LEU
A
80
62.469
−42.415
−11.652
1.00
17.25
A
N


ATOM
600
CA
LEU
A
80
61.433
−43.067
−10.833
1.00
17.10
A
C


ATOM
601
CB
LEU
A
80
60.588
−42.013
−10.113
1.00
18.65
A
C


ATOM
602
CG
LEU
A
80
61.318
−41.248
−9.018
1.00
18.83
A
C


ATOM
603
CD1
LEU
A
80
60.504
−40.080
−8.548
1.00
18.36
A
C


ATOM
604
CD2
LEU
A
80
61.697
−42.202
−7.848
1.00
16.67
A
C


ATOM
605
C
LEU
A
80
60.546
−43.948
−11.703
1.00
20.72
A
C


ATOM
606
O
LEU
A
80
60.159
−45.046
−11.299
1.00
22.37
A
O


ATOM
607
N
ASP
A
81
60.232
−43.466
−12.899
1.00
21.72
A
N


ATOM
608
CA
ASP
A
81
59.467
−44.254
−13.840
1.00
22.96
A
C


ATOM
609
CB
ASP
A
81
59.214
−43.462
−15.118
1.00
34.55
A
C


ATOM
610
CG
ASP
A
81
58.294
−44.203
−16.076
1.00
48.66
A
C


ATOM
611
OD1
ASP
A
81
57.059
−44.159
−15.876
1.00
47.13
A
O


ATOM
612
OD2
ASP
A
81
58.813
−44.858
−17.001
1.00
53.22
A
O


ATOM
613
C
ASP
A
81
60.161
−45.583
−14.159
1.00
26.16
A
C


ATOM
614
O
ASP
A
81
59.536
−46.638
−14.175
1.00
29.06
A
O


ATOM
615
N
ARG
A
82
61.465
−45.549
−14.398
1.00
25.91
A
N


ATOM
616
CA
ARG
A
82
62.185
−46.777
−14.703
1.00
26.78
A
C


ATOM
617
CB
ARG
A
82
63.562
−46.481
−15.281
1.00
34.17
A
C


ATOM
618
CG
ARG
A
82
63.492
−45.835
−16.668
1.00
42.87
A
C


ATOM
619
CD
ARG
A
82
64.892
−45.671
−17.266
1.00
49.93
A
C


ATOM
620
NE
ARG
A
82
65.763
−44.879
−16.390
1.00
44.21
A
N


ATOM
621
CZ
ARG
A
82
65.865
−43.549
−16.409
1.00
37.72
A
C


ATOM
622
NH1
ARG
A
82
65.166
−42.830
−17.278
1.00
35.82
A
N


ATOM
623
NH2
ARG
A
82
66.688
−42.932
−15.552
1.00
29.76
A
N


ATOM
624
C
ARG
A
82
62.309
−47.652
−13.485
1.00
29.77
A
C


ATOM
625
O
ARG
A
82
62.282
−48.887
−13.589
1.00
31.34
A
O


ATOM
626
N
PHE
A
83
62.440
−47.028
−12.321
1.00
24.68
A
N


ATOM
627
CA
PHE
A
83
62.444
−47.775
−11.076
1.00
25.58
A
C


ATOM
628
CB
PHE
A
83
62.638
−46.834
−9.889
1.00
25.29
A
C


ATOM
629
CG
PHE
A
83
62.662
−47.524
−8.546
1.00
25.27
A
C


ATOM
630
CD1
PHE
A
83
63.674
−48.403
−8.223
1.00
31.55
A
C


ATOM
631
CE1
PHE
A
83
63.705
−49.036
−6.959
1.00
33.76
A
C


ATOM
632
CZ
PHE
A
83
62.723
−48.761
−6.027
1.00
25.63
A
C


ATOM
633
CE2
PHE
A
83
61.688
−47.908
−6.363
1.00
23.21
A
C


ATOM
634
CD2
PHE
A
83
61.671
−47.275
−7.603
1.00
24.70
A
C


ATOM
635
C
PHE
A
83
61.153
−48.571
−10.920
1.00
23.30
A
C


ATOM
636
O
PHE
A
83
61.192
−49.699
−10.442
1.00
28.14
A
O


ATOM
637
N
VAL
A
84
60.008
−48.011
−11.304
1.00
22.28
A
N


ATOM
638
CA
VAL
A
84
58.766
−48.779
−11.191
1.00
24.82
A
C


ATOM
639
CB
VAL
A
84
57.544
−47.879
−11.140
1.00
34.14
A
C


ATOM
640
CG1
VAL
A
84
56.265
−48.737
−11.170
1.00
34.45
A
C


ATOM
641
CG2
VAL
A
84
57.610
−47.028
−9.895
1.00
24.63
A
C


ATOM
642
C
VAL
A
84
58.648
−49.778
−12.355
1.00
36.68
A
C


ATOM
643
O
VAL
A
84
58.466
−50.966
−12.117
1.00
28.56
A
O


ATOM
644
N
SER
A
85
58.821
−49.303
−13.592
1.00
37.14
A
N


ATOM
645
CA
ASER
A
85
58.620
−50.142
−14.794
0.50
37.10
A
C


ATOM
646
CA
BSER
A
85
58.622
−50.140
−14.791
0.50
39.01
A
C


ATOM
647
CB
ASER
A
85
58.845
−49.327
−16.072
0.50
32.00
A
C


ATOM
648
CB
BSER
A
85
58.830
−49.307
−16.061
0.50
36.25
A
C


ATOM
649
OG
ASER
A
85
60.229
−49.189
−16.324
0.50
28.69
A
O


ATOM
650
OG
BSER
A
85
58.016
−48.150
−16.027
0.50
36.93
A
O


ATOM
651
C
SER
A
85
59.536
−51.355
−14.824
1.00
37.47
A
C


ATOM
652
O
SER
A
85
59.177
−52.400
−15.354
1.00
41.76
A
O


ATOM
653
N
SER
A
86
60.725
−51.225
−14.260
1.00
36.59
A
N


ATOM
654
CA
SER
A
86
61.617
−52.354
−14.202
1.00
33.40
A
C


ATOM
655
CB
SER
A
86
63.043
−51.872
−13.933
1.00
32.66
A
C


ATOM
656
OG
SER
A
86
63.174
−51.413
−12.608
1.00
38.52
A
O


ATOM
657
C
SER
A
86
61.213
−53.407
−13.129
1.00
42.41
A
C


ATOM
658
O
SER
A
86
61.752
−54.504
−13.116
1.00
37.89
A
O


ATOM
659
N
GLY
A
87
60.286
−53.085
−12.229
1.00
46.93
A
N


ATOM
660
CA
GLY
A
87
60.046
−53.937
−11.038
1.00
46.12
A
C


ATOM
661
C
GLY
A
87
60.922
−53.651
−9.810
1.00
46.43
A
C


ATOM
662
O
GLY
A
87
60.874
−54.385
−8.823
1.00
42.81
A
O


ATOM
663
N
GLY
A
88
61.727
−52.591
−9.843
1.00
37.85
A
N


ATOM
664
CA
GLY
A
88
62.551
−52.234
−8.678
1.00
30.02
A
C


ATOM
665
C
GLY
A
88
61.734
−51.937
−7.421
1.00
27.92
A
C


ATOM
666
O
GLY
A
88
62.124
−52.306
−6.306
1.00
27.72
A
O


ATOM
667
N
PHE
A
89
60.592
−51.268
−7.592
1.00
28.80
A
N


ATOM
668
CA
PHE
A
89
59.756
−50.909
−6.437
1.00
28.02
A
C


ATOM
669
CB
PHE
A
89
58.610
−49.969
−6.826
1.00
26.31
A
C


ATOM
670
CG
PHE
A
89
57.765
−49.546
−5.651
1.00
25.79
A
C


ATOM
671
CD1
PHE
A
89
58.349
−48.920
−4.562
1.00
22.35
A
C


ATOM
672
CE1
PHE
A
89
57.592
−48.532
−3.464
1.00
24.18
A
C


ATOM
673
CZ
PHE
A
89
56.227
−48.769
−3.456
1.00
24.74
A
C


ATOM
674
CE2
PHE
A
89
55.637
−49.412
−4.520
1.00
29.64
A
C


ATOM
675
CD2
PHE
A
89
56.405
−49.802
−5.618
1.00
29.08
A
C


ATOM
676
C
PHE
A
89
59.204
−52.188
−5.830
1.00
30.78
A
C


ATOM
677
O
PHE
A
89
59.256
−52.396
−4.631
1.00
24.38
A
O


ATOM
678
N
ASP
A
90
58.703
−53.071
−6.677
1.00
34.48
A
N


ATOM
679
CA
ASP
A
90
58.272
−54.369
−6.178
1.00
39.02
A
C


ATOM
680
CB
ASP
A
90
57.660
−55.229
−7.270
1.00
44.69
A
C


ATOM
681
CG
ASP
A
90
56.172
−55.280
−7.138
1.00
62.48
A
C


ATOM
682
OD1
ASP
A
90
55.516
−54.248
−7.424
1.00
68.76
A
O


ATOM
683
OD2
ASP
A
90
55.680
−56.325
−6.662
1.00
53.84
A
O


ATOM
684
C
ASP
A
90
59.346
−55.114
−5.438
1.00
33.99
A
C


ATOM
685
O
ASP
A
90
59.083
−55.632
−4.369
1.00
40.51
A
O


ATOM
686
N
ALA
A
91
60.549
−55.144
−5.996
1.00
35.72
A
N


ATOM
687
CA
ALA
A
91
61.684
−55.738
−5.321
1.00
37.79
A
C


ATOM
688
CB
ALA
A
91
62.949
−55.644
−6.189
1.00
37.01
A
C


ATOM
689
C
ALA
A
91
61.926
−55.119
−3.934
1.00
39.48
A
C


ATOM
690
O
ALA
A
91
62.084
−55.874
−2.967
1.00
42.14
A
O


ATOM
691
N
VAL
A
92
61.958
−53.779
−3.795
1.00
30.00
A
N


ATOM
692
CA
VAL
A
92
62.289
−53.209
−2.463
1.00
21.89
A
C


ATOM
693
CB
VAL
A
92
62.599
−51.672
−2.377
1.00
31.87
A
C


ATOM
694
CG1
VAL
A
92
63.882
−51.298
−3.087
1.00
39.58
A
C


ATOM
695
CG2
VAL
A
92
61.418
−50.850
−2.847
1.00
18.63
A
C


ATOM
696
C
VAL
A
92
61.206
−53.499
−1.442
1.00
19.55
A
C


ATOM
697
O
VAL
A
92
61.519
−53.618
−0.261
1.00
21.56
A
O


ATOM
698
N
THR
A
93
59.947
−53.614
−1.854
1.00
21.70
A
N


ATOM
699
CA
THR
A
93
58.895
−53.860
−0.866
1.00
22.09
A
C


ATOM
700
CB
THR
A
93
57.459
−53.705
−1.425
1.00
25.99
A
C


ATOM
701
OG1
THR
A
93
57.160
−54.784
−2.301
1.00
26.79
A
O


ATOM
702
CG2
THR
A
93
57.287
−52.384
−2.193
1.00
25.32
A
C


ATOM
703
C
THR
A
93
59.092
−55.251
−0.213
1.00
29.92
A
C


ATOM
704
O
THR
A
93
58.533
−55.516
0.855
1.00
31.66
A
O


ATOM
705
N
LYS
A
94
59.903
−56.112
−0.832
1.00
33.47
A
N


ATOM
706
CA
LYS
A
94
60.181
−57.449
−0.275
1.00
35.78
A
C


ATOM
707
CB
LYS
A
94
60.334
−58.483
−1.392
1.00
37.52
A
C


ATOM
708
CG
LYS
A
94
59.155
−58.590
−2.351
1.00
44.02
A
C


ATOM
709
CD
LYS
A
94
57.883
−59.051
−1.649
1.00
53.84
A
C


ATOM
710
CE
LYS
A
94
57.079
−59.996
−2.537
1.00
64.77
A
C


ATOM
711
NZ
LYS
A
94
56.701
−59.367
−3.837
1.00
57.51
A
N


ATOM
712
C
LYS
A
94
61.444
−57.517
0.549
1.00
39.98
A
C


ATOM
713
O
LYS
A
94
61.650
−58.511
1.215
1.00
42.42
A
O


ATOM
714
N
THR
A
95
62.295
−56.488
0.507
1.00
33.33
A
N


ATOM
715
CA
ATHR
A
95
63.656
−56.589
1.055
0.50
35.43
A
C


ATOM
716
CA
BTHR
A
95
63.650
−56.590
1.067
0.50
33.00
A
C


ATOM
717
CB
ATHR
A
95
64.721
−56.623
−0.080
0.50
39.50
A
C


ATOM
718
CB
BTHR
A
95
64.721
−56.620
−0.047
0.50
33.60
A
C


ATOM
719
OG1
ATHR
A
95
64.258
−57.437
−1.168
0.50
40.50
A
O


ATOM
720
OG1
BTHR
A
95
64.663
−55.399
−0.793
0.50
26.73
A
O


ATOM
721
CG2
ATHR
A
95
66.052
−57.167
0.437
0.50
39.23
A
C


ATOM
722
CG2
BTHR
A
95
64.502
−57.798
−0.988
0.50
35.05
A
C


ATOM
723
C
THR
A
95
64.039
−55.461
2.013
1.00
39.07
A
C


ATOM
724
O
THR
A
95
64.768
−55.703
2.951
1.00
35.25
A
O


ATOM
725
N
SER
A
96
63.570
−54.219
1.763
1.00
29.44
A
N


ATOM
726
CA
SER
A
96
64.102
−53.048
2.502
1.00
27.71
A
C


ATOM
727
CB
SER
A
96
65.123
−52.359
1.584
1.00
30.94
A
C


ATOM
728
OG
SER
A
96
65.567
−51.129
2.099
1.00
26.14
A
O


ATOM
729
C
SER
A
96
63.040
−52.033
2.950
1.00
24.39
A
C


ATOM
730
O
SER
A
96
62.370
−51.436
2.111
1.00
22.56
A
O


ATOM
731
N
LEU
A
97
62.890
−51.820
4.254
1.00
19.43
A
N


ATOM
732
CA
LEU
A
97
61.966
−50.803
4.758
1.00
20.25
A
C


ATOM
733
CB
LEU
A
97
61.838
−50.828
6.286
1.00
21.08
A
C


ATOM
734
CG
LEU
A
97
60.990
−49.694
6.890
1.00
23.72
A
C


ATOM
735
CD1
LEU
A
97
59.524
−49.800
6.439
1.00
23.79
A
C


ATOM
736
CD2
LEU
A
97
61.130
−49.747
8.412
1.00
21.02
A
C


ATOM
737
C
LEU
A
97
62.449
−49.431
4.319
1.00
21.53
A
C


ATOM
738
O
LEU
A
97
61.667
−48.597
3.914
1.00
19.58
A
O


ATOM
739
N
HIS
A
98
63.749
−49.183
4.443
1.00
20.39
A
N


ATOM
740
CA
HIS
A
98
64.302
−47.908
4.040
1.00
23.40
A
C


ATOM
741
CB
HIS
A
98
65.811
−47.859
4.296
1.00
22.80
A
C


ATOM
742
CG
HIS
A
98
66.449
−46.591
3.812
1.00
26.94
A
C


ATOM
743
ND1
HIS
A
98
65.853
−45.383
3.956
1.00
31.63
A
N


ATOM
744
CE1
HIS
A
98
66.628
−44.426
3.408
1.00
26.07
A
C


ATOM
745
NE2
HIS
A
98
67.721
−45.016
2.904
1.00
39.12
A
N


ATOM
746
CD2
HIS
A
98
67.642
−46.358
3.136
1.00
41.20
A
C


ATOM
747
C
HIS
A
98
63.994
−47.632
2.578
1.00
18.12
A
C


ATOM
748
O
HIS
A
98
63.453
−46.575
2.221
1.00
18.83
A
O


ATOM
749
N
GLY
A
99
64.274
−48.605
1.728
1.00
19.83
A
N


ATOM
750
CA
GLY
A
99
64.028
−48.451
0.296
1.00
19.95
A
C


ATOM
751
C
GLY
A
99
62.559
−48.225
−0.023
1.00
19.49
A
C


ATOM
752
O
GLY
A
99
62.215
−47.410
−0.890
1.00
20.61
A
O


ATOM
753
N
THR
A
100
61.680
−48.937
0.682
1.00
18.03
A
N


ATOM
754
CA
THR
A
100
60.225
−48.836
0.422
1.00
16.81
A
C


ATOM
755
CB
THR
A
100
59.460
−49.948
1.171
1.00
19.24
A
C


ATOM
756
OG1
THR
A
100
59.892
−51.230
0.697
1.00
20.71
A
O


ATOM
757
CG2
THR
A
100
57.968
−49.818
0.950
1.00
15.65
A
C


ATOM
758
C
THR
A
100
59.681
−47.471
0.842
1.00
16.08
A
C


ATOM
759
O
THR
A
100
58.909
−46.822
0.109
1.00
15.43
A
O


ATOM
760
N
ALA
A
101
60.054
−47.040
2.038
1.00
16.07
A
N


ATOM
761
CA
ALA
A
101
59.614
−45.746
2.551
1.00
18.24
A
C


ATOM
762
CB
ALA
A
101
59.971
−45.571
4.067
1.00
15.42
A
C


ATOM
763
C
ALA
A
101
60.133
−44.569
1.731
1.00
16.77
A
C


ATOM
764
O
ALA
A
101
59.395
−43.610
1.464
1.00
17.48
A
O


ATOM
765
N
LEU
A
102
61.397
−44.630
1.354
1.00
19.20
A
N


ATOM
766
CA
LEU
A
102
61.989
−43.597
0.558
1.00
17.07
A
C


ATOM
767
CB
LEU
A
102
63.514
−43.759
0.447
1.00
19.30
A
C


ATOM
768
CG
LEU
A
102
64.275
−42.701
−0.339
1.00
20.35
A
C


ATOM
769
CD1
LEU
A
102
63.946
−41.212
0.095
1.00
18.24
A
C


ATOM
770
CD2
LEU
A
102
65.767
−42.977
−0.157
1.00
21.12
A
C


ATOM
771
C
LEU
A
102
61.350
−43.544
−0.813
1.00
19.36
A
C


ATOM
772
O
LEU
A
102
60.910
−42.479
−1.258
1.00
18.06
A
O


ATOM
773
N
SER
A
103
61.256
−44.686
−1.470
1.00
15.50
A
N


ATOM
774
CA
SER
A
103
60.689
−44.690
−2.798
1.00
17.71
A
C


ATOM
775
CB
SER
A
103
61.019
−46.015
−3.528
1.00
18.18
A
C


ATOM
776
OG
SER
A
103
60.455
−47.104
−2.871
1.00
17.47
A
O


ATOM
777
C
SER
A
103
59.188
−44.382
−2.765
1.00
15.07
A
C


ATOM
778
O
SER
A
103
58.667
−43.712
−3.651
1.00
16.88
A
O


ATOM
779
N
PHE
A
104
58.496
−44.820
−1.719
1.00
17.99
A
N


ATOM
780
CA
PHE
A
104
57.087
−44.477
−1.573
1.00
15.40
A
C


ATOM
781
CB
PHE
A
104
56.492
−45.119
−0.305
1.00
17.80
A
C


ATOM
782
CG
PHE
A
104
55.047
−44.773
−0.062
1.00
17.24
A
C


ATOM
783
CD1
PHE
A
104
54.693
−43.584
0.512
1.00
14.51
A
C


ATOM
784
CE1
PHE
A
104
53.343
−43.270
0.748
1.00
18.89
A
C


ATOM
785
CZ
PHE
A
104
52.349
−44.154
0.380
1.00
14.12
A
C


ATOM
786
CE2
PHE
A
104
52.699
−45.359
−0.165
1.00
18.32
A
C


ATOM
787
CD2
PHE
A
104
54.028
−45.674
−0.395
1.00
19.18
A
C


ATOM
788
C
PHE
A
104
56.889
−42.949
−1.573
1.00
14.21
A
C


ATOM
789
O
PHE
A
104
56.015
−42.433
−2.269
1.00
13.20
A
O


ATOM
790
N
ARG
A
105
57.667
−42.247
−0.765
1.00
15.27
A
N


ATOM
791
CA
ARG
A
105
57.594
−40.801
−0.677
1.00
14.68
A
C


ATOM
792
CB
ARG
A
105
58.499
−40.268
0.421
1.00
17.00
A
C


ATOM
793
CG
ARG
A
105
58.556
−38.738
0.484
1.00
19.27
A
C


ATOM
794
CD
ARG
A
105
59.130
−38.281
1.770
1.00
17.80
A
C


ATOM
795
NE
ARG
A
105
60.520
−38.658
1.963
1.00
17.60
A
N


ATOM
796
CZ
ARG
A
105
61.557
−38.046
1.397
1.00
22.34
A
C


ATOM
797
NH1
ARG
A
105
61.364
−37.013
0.572
1.00
19.72
A
N


ATOM
798
NH2
ARG
A
105
62.788
−38.451
1.669
1.00
21.62
A
N


ATOM
799
C
ARG
A
105
57.937
−40.152
−2.012
1.00
13.92
A
C


ATOM
800
O
ARG
A
105
57.207
−39.275
−2.486
1.00
14.65
A
O


ATOM
801
N
LEU
A
106
59.043
−40.581
−2.615
1.00
13.96
A
N


ATOM
802
CA
LEU
A
106
59.471
−39.954
−3.871
1.00
16.38
A
C


ATOM
803
CB
LEU
A
106
60.839
−40.462
−4.268
1.00
16.84
A
C


ATOM
804
CG
LEU
A
106
61.981
−40.096
−3.297
1.00
16.96
A
C


ATOM
805
CD1
LEU
A
106
63.275
−40.704
−3.883
1.00
19.16
A
C


ATOM
806
CD2
LEU
A
106
62.114
−38.588
−3.196
1.00
14.34
A
C


ATOM
807
C
LEU
A
106
58.466
−40.183
−4.994
1.00
14.71
A
C


ATOM
808
O
LEU
A
106
58.182
−39.290
−5.790
1.00
15.17
A
O


ATOM
809
N
LEU
A
107
57.956
−41.391
−5.073
1.00
15.41
A
N


ATOM
810
CA
LEU
A
107
56.965
−41.740
−6.101
1.00
16.47
A
C


ATOM
811
CB
LEU
A
107
56.653
−43.241
−6.076
1.00
14.69
A
C


ATOM
812
CG
LEU
A
107
57.725
−44.138
−6.672
1.00
17.19
A
C


ATOM
813
CD1
LEU
A
107
57.512
−45.649
−6.276
1.00
18.03
A
C


ATOM
814
CD2
LEU
A
107
57.844
−43.928
−8.194
1.00
16.63
A
C


ATOM
815
C
LEU
A
107
55.711
−40.922
−5.932
1.00
15.60
A
C


ATOM
816
O
LEU
A
107
55.210
−40.309
−6.903
1.00
16.24
A
O


ATOM
817
N
ARG
A
108
55.180
−40.886
−4.714
1.00
15.68
A
N


ATOM
818
CA
ARG
A
108
53.935
−40.108
−4.511
1.00
17.82
A
C


ATOM
819
CB
ARG
A
108
53.336
−40.378
−3.139
1.00
16.80
A
C


ATOM
820
CG
ARG
A
108
52.034
−39.599
−2.956
1.00
20.62
A
C


ATOM
821
CD
ARG
A
108
51.224
−40.093
−1.772
1.00
21.54
A
C


ATOM
822
NE
ARG
A
108
50.578
−41.371
−2.062
1.00
15.38
A
N


ATOM
823
CZ
ARG
A
108
49.816
−42.031
−1.185
1.00
17.50
A
C


ATOM
824
NH1
ARG
A
108
49.653
−41.589
0.055
1.00
17.78
A
N


ATOM
825
NH2
ARG
A
108
49.260
−43.161
−1.528
1.00
16.16
A
N


ATOM
826
C
ARG
A
108
54.151
−38.613
−4.684
1.00
17.00
A
C


ATOM
827
O
ARG
A
108
53.341
−37.933
−5.282
1.00
15.18
A
O


ATOM
828
N
GLN
A
109
55.282
−38.102
−4.198
1.00
15.43
A
N


ATOM
829
CA
GLN
A
109
55.646
−36.687
−4.480
1.00
15.25
A
C


ATOM
830
CB
GLN
A
109
57.057
−36.399
−3.970
1.00
18.89
A
C


ATOM
831
CG
GLN
A
109
57.597
−35.012
−4.344
1.00
21.63
A
C


ATOM
832
CD
GLN
A
109
59.039
−34.827
−3.929
1.00
26.03
A
C


ATOM
833
OE1
GLN
A
109
59.661
−35.719
−3.314
1.00
20.78
A
O


ATOM
834
NE2
GLN
A
109
59.581
−33.631
−4.227
1.00
23.99
A
N


ATOM
835
C
GLN
A
109
55.597
−36.324
−5.991
1.00
18.63
A
C


ATOM
836
O
GLN
A
109
55.168
−35.217
−6.367
1.00
16.12
A
O


ATOM
837
N
HIS
A
110
56.041
−37.249
−6.829
1.00
14.77
A
N


ATOM
838
CA
HIS
A
110
56.118
−37.033
−8.254
1.00
18.76
A
C


ATOM
839
CB
HIS
A
110
57.440
−37.571
−8.778
1.00
18.80
A
C


ATOM
840
CG
HIS
A
110
58.607
−36.694
−8.382
1.00
19.46
A
C


ATOM
841
ND1
HIS
A
110
58.915
−35.561
−9.062
1.00
16.75
A
N


ATOM
842
CE1
HIS
A
110
59.958
−34.950
−8.464
1.00
22.03
A
C


ATOM
843
NE2
HIS
A
110
60.273
−35.659
−7.354
1.00
18.94
A
N


ATOM
844
CD2
HIS
A
110
59.454
−36.747
−7.293
1.00
14.42
A
C


ATOM
845
C
HIS
A
110
54.906
−37.567
−8.983
1.00
21.66
A
C


ATOM
846
O
HIS
A
110
54.937
−37.766
−10.194
1.00
22.45
A
O


ATOM
847
N
GLY
A
111
53.809
−37.778
−8.244
1.00
19.21
A
N


ATOM
848
CA
GLY
A
111
52.491
−38.034
−8.880
1.00
18.67
A
C


ATOM
849
C
GLY
A
111
52.232
−39.478
−9.221
1.00
20.43
A
C


ATOM
850
O
GLY
A
111
51.223
−39.775
−9.806
1.00
18.90
A
O


ATOM
851
N
PHE
A
112
53.133
−40.398
−8.881
1.00
16.53
A
N


ATOM
852
CA
PHE
A
112
52.923
−41.809
−9.224
1.00
18.88
A
C


ATOM
853
CB
PHE
A
112
54.206
−42.618
−9.179
1.00
19.65
A
C


ATOM
854
CG
PHE
A
112
55.188
−42.250
−10.259
1.00
23.54
A
C


ATOM
855
CD1
PHE
A
112
56.062
−41.200
−10.077
1.00
20.65
A
C


ATOM
856
CE1
PHE
A
112
56.994
−40.852
−11.057
1.00
25.46
A
C


ATOM
857
CZ
PHE
A
112
57.021
−41.536
−12.241
1.00
28.69
A
C


ATOM
858
CE2
PHE
A
112
56.124
−42.597
−12.452
1.00
31.25
A
C


ATOM
859
CD2
PHE
A
112
55.224
−42.953
−11.457
1.00
29.23
A
C


ATOM
860
C
PHE
A
112
51.987
−42.405
−8.200
1.00
23.31
A
C


ATOM
861
O
PHE
A
112
51.922
−41.949
−7.065
1.00
20.49
A
O


ATOM
862
N
GLU
A
113
51.300
−43.461
−8.614
1.00
24.49
A
N


ATOM
863
CA
GLU
A
113
50.309
−44.144
−7.796
1.00
25.45
A
C


ATOM
864
CB
GLU
A
113
49.280
−44.839
−8.720
1.00
37.67
A
C


ATOM
865
CG
GLU
A
113
47.845
−44.840
−8.204
1.00
58.64
A
C


ATOM
866
CD
GLU
A
113
46.794
−45.024
−9.330
1.00
81.44
A
C


ATOM
867
OE1
GLU
A
113
46.982
−45.896
−10.209
1.00
68.75
A
O


ATOM
868
OE2
GLU
A
113
45.775
−44.293
−9.335
1.00
86.73
A
O


ATOM
869
C
GLU
A
113
51.001
−45.194
−6.966
1.00
23.08
A
C


ATOM
870
O
GLU
A
113
51.580
−46.140
−7.493
1.00
20.88
A
O


ATOM
871
N
VAL
A
114
50.965
−45.033
−5.651
1.00
19.99
A
N


ATOM
872
CA
VAL
A
114
51.518
−46.025
−4.740
1.00
16.88
A
C


ATOM
873
CB
VAL
A
114
52.871
−45.623
−4.092
1.00
21.01
A
C


ATOM
874
CG1
VAL
A
114
54.053
−45.879
−5.042
1.00
25.38
A
C


ATOM
875
CG2
VAL
A
114
52.874
−44.171
−3.610
1.00
15.30
A
C


ATOM
876
C
VAL
A
114
50.478
−46.235
−3.655
1.00
19.88
A
C


ATOM
877
O
VAL
A
114
49.692
−45.345
−3.373
1.00
17.02
A
O


ATOM
878
N
SER
A
115
50.457
−47.431
−3.086
1.00
18.74
A
N


ATOM
879
CA
SER
A
115
49.473
−47.822
−2.108
1.00
20.78
A
C


ATOM
880
CB
SER
A
115
48.783
−49.095
−2.633
1.00
28.67
A
C


ATOM
881
OG
SER
A
115
48.317
−49.928
−1.594
1.00
27.83
A
O


ATOM
882
C
SER
A
115
50.108
−48.124
−0.765
1.00
15.47
A
C


ATOM
883
O
SER
A
115
51.221
−48.652
−0.693
1.00
18.09
A
O


ATOM
884
N
GLN
A
116
49.376
−47.894
0.318
1.00
17.33
A
N


ATOM
885
CA
GLN
A
116
49.869
−48.283
1.669
1.00
18.18
A
C


ATOM
886
CB
GLN
A
116
48.875
−47.859
2.754
1.00
19.83
A
C


ATOM
887
CG
GLN
A
116
47.500
−48.590
2.766
1.00
19.69
A
C


ATOM
888
CD
GLN
A
116
46.636
−48.180
3.948
1.00
19.13
A
C


ATOM
889
OE1
GLN
A
116
47.048
−47.385
4.805
1.00
17.20
A
O


ATOM
890
NE2
GLN
A
116
45.449
−48.739
4.019
1.00
23.50
A
N


ATOM
891
C
GLN
A
116
50.179
−49.768
1.813
1.00
21.60
A
C


ATOM
892
O
GLN
A
116
50.854
−50.171
2.766
1.00
18.86
A
O


ATOM
893
N
GLU
A
117
49.703
−50.605
0.882
1.00
16.60
A
N


ATOM
894
CA
GLU
A
117
50.018
−52.032
0.917
1.00
18.43
A
C


ATOM
895
CB
GLU
A
117
49.217
−52.835
−0.141
1.00
23.92
A
C


ATOM
896
CG
GLU
A
117
47.699
−52.752
0.019
1.00
28.14
A
C


ATOM
897
CD
GLU
A
117
46.922
−53.442
−1.115
1.00
42.18
A
C


ATOM
898
OE1
GLU
A
117
47.415
−54.462
−1.646
1.00
41.50
A
O


ATOM
899
OE2
GLU
A
117
45.818
−52.963
−1.482
1.00
41.18
A
O


ATOM
900
C
GLU
A
117
51.502
−52.288
0.664
1.00
21.27
A
C


ATOM
901
O
GLU
A
117
51.981
−53.374
0.962
1.00
19.92
A
O


ATOM
902
N
ALA
A
118
52.227
−51.315
0.111
1.00
19.55
A
N


ATOM
903
CA
ALA
A
118
53.695
−51.444
−0.034
1.00
22.80
A
C


ATOM
904
CB
ALA
A
118
54.294
−50.191
−0.610
1.00
18.03
A
C


ATOM
905
C
ALA
A
118
54.403
−51.768
1.300
1.00
20.01
A
C


ATOM
906
O
ALA
A
118
55.498
−52.326
1.310
1.00
20.69
A
O


ATOM
907
N
PHE
A
119
53.786
−51.388
2.412
1.00
19.91
A
N


ATOM
908
CA
PHE
A
119
54.346
−51.646
3.740
1.00
18.27
A
C


ATOM
909
CB
PHE
A
119
53.972
−50.475
4.640
1.00
19.18
A
C


ATOM
910
CG
PHE
A
119
54.607
−49.177
4.191
1.00
22.08
A
C


ATOM
911
CD1
PHE
A
119
55.972
−48.965
4.382
1.00
17.84
A
C


ATOM
912
CE1
PHE
A
119
56.570
−47.809
3.937
1.00
20.07
A
C


ATOM
913
CZ
PHE
A
119
55.815
−46.844
3.280
1.00
19.50
A
C


ATOM
914
CE2
PHE
A
119
54.461
−47.059
3.050
1.00
22.32
A
C


ATOM
915
CD2
PHE
A
119
53.860
−48.217
3.523
1.00
20.27
A
C


ATOM
916
C
PHE
A
119
53.925
−52.953
4.410
1.00
19.87
A
C


ATOM
917
O
PHE
A
119
54.400
−53.271
5.514
1.00
22.50
A
O


ATOM
918
N
SER
A
120
53.061
−53.726
3.766
1.00
24.30
A
N


ATOM
919
CA
SER
A
120
52.478
−54.936
4.418
1.00
25.36
A
C


ATOM
920
CB
SER
A
120
51.376
−55.544
3.542
1.00
29.84
A
C


ATOM
921
OG
SER
A
120
51.975
−56.159
2.408
1.00
29.25
A
O


ATOM
922
C
SER
A
120
53.508
−56.013
4.769
1.00
27.38
A
C


ATOM
923
O
SER
A
120
53.388
−56.673
5.790
1.00
37.36
A
O


ATOM
924
N
GLY
A
121
54.529
−56.169
3.940
1.00
28.63
A
N


ATOM
925
CA
GLY
A
121
55.647
−57.065
4.236
1.00
29.21
A
C


ATOM
926
C
GLY
A
121
56.476
−56.757
5.498
1.00
34.22
A
C


ATOM
927
O
GLY
A
121
57.318
−57.556
5.871
1.00
30.84
A
O


ATOM
928
N
PHE
A
122
56.255
−55.625
6.167
1.00
29.27
A
N


ATOM
929
CA
PHE
A
122
57.066
−55.273
7.353
1.00
33.84
A
C


ATOM
930
CB
PHE
A
122
57.660
−53.861
7.177
1.00
32.20
A
C


ATOM
931
CG
PHE
A
122
58.428
−53.715
5.909
1.00
29.16
A
C


ATOM
932
CD1
PHE
A
122
59.689
−54.290
5.789
1.00
25.93
A
C


ATOM
933
CE1
PHE
A
122
60.388
−54.214
4.619
1.00
25.17
A
C


ATOM
934
CZ
PHE
A
122
59.840
−53.563
3.519
1.00
26.81
A
C


ATOM
935
CE2
PHE
A
122
58.576
−53.009
3.606
1.00
22.72
A
C


ATOM
936
CD2
PHE
A
122
57.865
−53.090
4.803
1.00
23.54
A
C


ATOM
937
C
PHE
A
122
56.295
−55.360
8.661
1.00
31.14
A
C


ATOM
938
O
PHE
A
122
56.692
−54.770
9.656
1.00
26.44
A
O


ATOM
939
N
LYS
A
123
55.192
−56.098
8.667
1.00
39.19
A
N


ATOM
940
CA
LYS
A
123
54.360
−56.188
9.851
1.00
38.73
A
C


ATOM
941
CB
LYS
A
123
52.932
−55.746
9.513
1.00
47.70
A
C


ATOM
942
CG
LYS
A
123
52.836
−54.210
9.399
1.00
45.24
A
C


ATOM
943
CD
LYS
A
123
51.787
−53.696
8.414
1.00
64.96
A
C


ATOM
944
CE
LYS
A
123
50.467
−53.329
9.085
1.00
67.08
A
C


ATOM
945
NZ
LYS
A
123
50.584
−52.199
10.038
1.00
69.25
A
N


ATOM
946
C
LYS
A
123
54.477
−57.590
10.466
1.00
43.53
A
C


ATOM
947
O
LYS
A
123
54.903
−58.527
9.796
1.00
37.81
A
O


ATOM
948
N
ASP
A
124
54.210
−57.698
11.766
1.00
38.56
A
N


ATOM
949
CA
ASP
A
124
54.230
−58.999
12.466
1.00
39.95
A
C


ATOM
950
CB
ASP
A
124
54.587
−58.824
13.956
1.00
33.62
A
C


ATOM
951
CG
ASP
A
124
53.546
−58.010
14.740
1.00
39.27
A
C


ATOM
952
OD1
ASP
A
124
52.345
−58.051
14.417
1.00
40.78
A
O


ATOM
953
OD2
ASP
A
124
53.934
−57.317
15.698
1.00
44.37
A
O


ATOM
954
C
ASP
A
124
52.888
−59.715
12.305
1.00
42.17
A
C


ATOM
955
O
ASP
A
124
51.982
−59.208
11.641
1.00
30.57
A
O


ATOM
956
N
GLN
A
125
52.767
−60.897
12.910
1.00
50.65
A
N


ATOM
957
CA
GLN
A
125
51.522
−61.676
12.898
1.00
53.07
A
C


ATOM
958
CB
GLN
A
125
51.649
−62.870
13.837
1.00
54.97
A
C


ATOM
959
CG
GLN
A
125
52.666
−63.899
13.383
1.00
73.54
A
C


ATOM
960
CD
GLN
A
125
52.683
−65.136
14.270
1.00
83.04
A
C


ATOM
961
OE1
GLN
A
125
51.999
−65.200
15.297
1.00
76.17
A
O


ATOM
962
NE2
GLN
A
125
53.467
−66.130
13.871
1.00
79.73
A
N


ATOM
963
C
GLN
A
125
50.297
−60.856
13.305
1.00
49.63
A
C


ATOM
964
O
GLN
A
125
49.229
−60.995
12.715
1.00
52.74
A
O


ATOM
965
N
ASN
A
126
50.461
−59.997
14.309
1.00
45.11
A
N


ATOM
966
CA
ASN
A
126
49.374
−59.122
14.775
1.00
43.91
A
C


ATOM
967
CB
ASN
A
126
49.619
−58.769
16.235
1.00
42.50
A
C


ATOM
968
CG
ASN
A
126
49.596
−59.982
17.103
1.00
45.99
A
C


ATOM
969
OD1
ASN
A
126
48.573
−60.658
17.196
1.00
47.17
A
O


ATOM
970
ND2
ASN
A
126
50.732
−60.305
17.709
1.00
43.81
A
N


ATOM
971
C
ASN
A
126
49.128
−57.835
13.976
1.00
44.06
A
C


ATOM
972
O
ASN
A
126
48.339
−56.983
14.403
1.00
47.73
A
O


ATOM
973
N
GLY
A
127
49.792
−57.684
12.831
1.00
43.62
A
N


ATOM
974
CA
GLY
A
127
49.616
−56.490
11.994
1.00
44.43
A
C


ATOM
975
C
GLY
A
127
50.232
−55.197
12.530
1.00
47.04
A
C


ATOM
976
O
GLY
A
127
49.812
−54.100
12.156
1.00
47.00
A
O


ATOM
977
N
ASN
A
128
51.230
−55.319
13.399
1.00
37.90
A
N


ATOM
978
CA
ASN
A
128
51.984
−54.167
13.866
1.00
36.92
A
C


ATOM
979
CB
ASN
A
128
52.093
−54.194
15.390
1.00
39.55
A
C


ATOM
980
CG
ASN
A
128
50.737
−54.083
16.064
1.00
42.96
A
C


ATOM
981
OD1
ASN
A
128
49.899
−53.270
15.669
1.00
46.29
A
O


ATOM
982
ND2
ASN
A
128
50.505
−54.914
17.065
1.00
42.44
A
N


ATOM
983
C
ASN
A
128
53.354
−54.202
13.209
1.00
35.45
A
C


ATOM
984
O
ASN
A
128
53.845
−55.273
12.874
1.00
34.29
A
O


ATOM
985
N
PHE
A
129
53.963
−53.038
12.987
1.00
32.75
A
N


ATOM
986
CA
PHE
A
129
55.312
−53.016
12.427
1.00
28.49
A
C


ATOM
987
CB
PHE
A
129
55.810
−51.580
12.253
1.00
29.22
A
C


ATOM
988
CG
PHE
A
129
55.204
−50.885
11.092
1.00
29.65
A
C


ATOM
989
CD1
PHE
A
129
55.786
−50.992
9.836
1.00
27.09
A
C


ATOM
990
CE1
PHE
A
129
55.219
−50.342
8.749
1.00
31.01
A
C


ATOM
991
CZ
PHE
A
129
54.041
−49.610
8.912
1.00
28.34
A
C


ATOM
992
CE2
PHE
A
129
53.452
−49.507
10.162
1.00
30.20
A
C


ATOM
993
CD2
PHE
A
129
54.031
−50.146
11.245
1.00
28.89
A
C


ATOM
994
C
PHE
A
129
56.253
−53.813
13.341
1.00
28.78
A
C


ATOM
995
O
PHE
A
129
56.181
−53.707
14.560
1.00
29.83
A
O


ATOM
996
N
LEU
A
130
57.101
−54.628
12.731
1.00
32.19
A
N


ATOM
997
CA
LEU
A
130
58.044
−55.487
13.451
1.00
35.11
A
C


ATOM
998
CB
LEU
A
130
58.918
−56.250
12.457
1.00
35.91
A
C


ATOM
999
CG
LEU
A
130
58.165
−57.323
11.675
1.00
38.40
A
C


ATOM
1000
CD1
LEU
A
130
58.839
−57.597
10.323
1.00
34.13
A
C


ATOM
1001
CD2
LEU
A
130
58.069
−58.567
12.544
1.00
38.09
A
C


ATOM
1002
C
LEU
A
130
58.934
−54.660
14.360
1.00
37.04
A
C


ATOM
1003
O
LEU
A
130
59.544
−53.674
13.929
1.00
31.94
A
O


ATOM
1004
N
GLU
A
131
59.007
−55.079
15.615
1.00
34.55
A
N


ATOM
1005
CA
GLU
A
131
59.765
−54.383
16.651
1.00
39.68
A
C


ATOM
1006
CB
GLU
A
131
59.755
−55.210
17.955
1.00
38.76
A
C


ATOM
1007
CG
GLU
A
131
60.141
−54.423
19.214
1.00
49.60
A
C


ATOM
1008
CD
GLU
A
131
59.132
−53.343
19.593
1.00
56.31
A
C


ATOM
1009
OE1
GLU
A
131
58.053
−53.260
18.966
1.00
67.91
A
O


ATOM
1010
OE2
GLU
A
131
59.417
−52.571
20.528
1.00
59.92
A
O


ATOM
1011
C
GLU
A
131
61.206
−54.119
16.260
1.00
30.94
A
C


ATOM
1012
O
GLU
A
131
61.700
−53.017
16.467
1.00
34.37
A
O


ATOM
1013
N
ASN
A
132
61.858
−55.131
15.686
1.00
31.21
A
N


ATOM
1014
CA
AASN
A
132
63.297
−55.058
15.372
0.50
30.95
A
C


ATOM
1015
CA
BASN
A
132
63.289
−55.084
15.356
0.50
34.00
A
C


ATOM
1016
CB
AASN
A
132
63.837
−56.433
15.001
0.50
31.32
A
C


ATOM
1017
CB
BASN
A
132
63.799
−56.491
14.994
0.50
39.09
A
C


ATOM
1018
CG
AASN
A
132
63.341
−56.903
13.653
0.50
33.96
A
C


ATOM
1019
CG
BASN
A
132
62.692
−57.417
14.481
0.50
47.90
A
C


ATOM
1020
OD1
AASN
A
132
62.496
−56.254
13.027
0.50
27.33
A
O


ATOM
1021
OD1
BASN
A
132
61.662
−57.614
15.141
0.50
44.58
A
O


ATOM
1022
ND2
AASN
A
132
63.853
−58.047
13.199
0.50
32.74
A
N


ATOM
1023
ND2
BASN
A
132
62.911
−57.999
13.307
0.50
52.36
A
N


ATOM
1024
C
ASN
A
132
63.664
−54.083
14.250
1.00
40.19
A
C


ATOM
1025
O
ASN
A
132
64.858
−53.821
14.013
1.00
32.34
A
O


ATOM
1026
N
LEU
A
133
62.659
−53.549
13.546
1.00
31.58
A
N


ATOM
1027
CA
LEU
A
133
62.917
−52.495
12.573
1.00
31.84
A
C


ATOM
1028
CB
LEU
A
133
61.659
−52.164
11.744
1.00
29.65
A
C


ATOM
1029
CG
LEU
A
133
61.133
−53.293
10.858
1.00
32.70
A
C


ATOM
1030
CD1
LEU
A
133
59.694
−52.974
10.379
1.00
32.29
A
C


ATOM
1031
CD2
LEU
A
133
62.069
−53.575
9.682
1.00
33.92
A
C


ATOM
1032
C
LEU
A
133
63.403
−51.237
13.314
1.00
31.94
A
C


ATOM
1033
O
LEU
A
133
64.048
−50.367
12.708
1.00
31.35
A
O


ATOM
1034
N
LYS
A
134
63.109
−51.143
14.617
1.00
30.73
A
N


ATOM
1035
CA
LYS
A
134
63.553
−49.996
15.427
1.00
32.93
A
C


ATOM
1036
CB
LYS
A
134
63.018
−50.085
16.850
1.00
38.53
A
C


ATOM
1037
CG
LYS
A
134
63.773
−51.080
17.716
1.00
42.00
A
C


ATOM
1038
CD
LYS
A
134
63.062
−51.357
19.013
1.00
41.05
A
C


ATOM
1039
CE
LYS
A
134
63.823
−52.399
19.828
1.00
41.23
A
C


ATOM
1040
NZ
LYS
A
134
63.541
−52.187
21.252
1.00
33.39
A
N


ATOM
1041
C
LYS
A
134
65.084
−49.841
15.462
1.00
31.46
A
C


ATOM
1042
O
LYS
A
134
65.575
−48.762
15.719
1.00
33.74
A
O


ATOM
1043
N
GLU
A
135
65.819
−50.909
15.171
1.00
31.04
A
N


ATOM
1044
CA
GLU
A
135
67.285
−50.863
15.147
1.00
40.52
A
C


ATOM
1045
CB
GLU
A
135
67.896
−52.285
15.148
1.00
46.31
A
C


ATOM
1046
CG
GLU
A
135
67.394
−53.259
16.234
1.00
52.23
A
C


ATOM
1047
CD
GLU
A
135
67.544
−52.748
17.666
1.00
65.02
A
C


ATOM
1048
OE1
GLU
A
135
68.300
−51.773
17.907
1.00
55.43
A
O


ATOM
1049
OE2
GLU
A
135
66.894
−53.344
18.559
1.00
73.29
A
O


ATOM
1050
C
GLU
A
135
67.836
−50.121
13.928
1.00
40.04
A
C


ATOM
1051
O
GLU
A
135
68.993
−49.709
13.920
1.00
37.69
A
O


ATOM
1052
N
ASP
A
136
67.023
−49.974
12.890
1.00
37.22
A
N


ATOM
1053
CA
ASP
A
136
67.463
−49.352
11.647
1.00
30.48
A
C


ATOM
1054
CB
ASP
A
136
66.916
−50.172
10.481
1.00
32.94
A
C


ATOM
1055
CG
ASP
A
136
67.367
−49.665
9.131
1.00
36.65
A
C


ATOM
1056
OD1
ASP
A
136
67.881
−48.517
9.013
1.00
35.76
A
O


ATOM
1057
OD2
ASP
A
136
67.167
−50.431
8.174
1.00
34.74
A
O


ATOM
1058
C
ASP
A
136
66.961
−47.907
11.616
1.00
33.39
A
C


ATOM
1059
O
ASP
A
136
65.886
−47.611
11.068
1.00
24.86
A
O


ATOM
1060
N
ILE
A
137
67.749
−47.001
12.191
1.00
24.88
A
N


ATOM
1061
CA
ILE
A
137
67.290
−45.633
12.403
1.00
24.01
A
C


ATOM
1062
CB
ILE
A
137
68.288
−44.799
13.186
1.00
26.01
A
C


ATOM
1063
CG1
ILE
A
137
68.536
−45.436
14.570
1.00
36.15
A
C


ATOM
1064
CD1
ILE
A
137
67.278
−45.976
15.270
1.00
28.86
A
C


ATOM
1065
CG2
ILE
A
137
67.802
−43.339
13.300
1.00
23.28
A
C


ATOM
1066
C
ILE
A
137
66.954
−44.931
11.089
1.00
27.36
A
C


ATOM
1067
O
ILE
A
137
65.990
−44.175
11.016
1.00
22.50
A
O


ATOM
1068
N
LYS
A
138
67.742
−45.203
10.068
1.00
25.84
A
N


ATOM
1069
CA
LYS
A
138
67.586
−44.599
8.758
1.00
30.98
A
C


ATOM
1070
CB
LYS
A
138
68.696
−45.145
7.867
1.00
35.77
A
C


ATOM
1071
CG
LYS
A
138
69.312
−44.193
6.894
1.00
51.75
A
C


ATOM
1072
CD
LYS
A
138
70.032
−44.990
5.790
1.00
68.67
A
C


ATOM
1073
CE
LYS
A
138
71.089
−44.167
5.070
1.00
82.46
A
C


ATOM
1074
NZ
LYS
A
138
72.392
−44.194
5.792
1.00
86.22
A
N


ATOM
1075
C
LYS
A
138
66.216
−44.970
8.165
1.00
24.80
A
C


ATOM
1076
O
LYS
A
138
65.544
−44.145
7.542
1.00
23.16
A
O


ATOM
1077
N
ALA
A
139
65.859
−46.241
8.317
1.00
22.69
A
N


ATOM
1078
CA
ALA
A
139
64.604
−46.794
7.816
1.00
19.20
A
C


ATOM
1079
CB
ALA
A
139
64.639
−48.355
7.913
1.00
20.06
A
C


ATOM
1080
C
ALA
A
139
63.437
−46.208
8.611
1.00
22.32
A
C


ATOM
1081
O
ALA
A
139
62.407
−45.830
8.055
1.00
22.17
A
O


ATOM
1082
N
ILE
A
140
63.600
−46.083
9.917
1.00
19.58
A
N


ATOM
1083
CA
ILE
A
140
62.562
−45.451
10.730
1.00
23.10
A
C


ATOM
1084
CB
ILE
A
140
62.813
−45.616
12.252
1.00
25.60
A
C


ATOM
1085
CG1
ILE
A
140
62.907
−47.096
12.627
1.00
31.81
A
C


ATOM
1086
CD1
ILE
A
140
61.752
−47.904
12.154
1.00
32.36
A
C


ATOM
1087
CG2
ILE
A
140
61.679
−44.993
13.027
1.00
29.25
A
C


ATOM
1088
C
ILE
A
140
62.357
−43.966
10.391
1.00
19.14
A
C


ATOM
1089
O
ILE
A
140
61.212
−43.517
10.314
1.00
19.83
A
O


ATOM
1090
N
LEU
A
141
63.438
−43.206
10.198
1.00
16.95
A
N


ATOM
1091
CA
LEU
A
141
63.317
−41.813
9.748
1.00
21.76
A
C


ATOM
1092
CB
LEU
A
141
64.682
−41.174
9.505
1.00
23.37
A
C


ATOM
1093
CG
LEU
A
141
65.294
−40.491
10.709
1.00
29.38
A
C


ATOM
1094
CD1
LEU
A
141
66.716
−40.101
10.366
1.00
26.91
A
C


ATOM
1095
CD2
LEU
A
141
64.434
−39.292
11.112
1.00
25.25
A
C


ATOM
1096
C
LEU
A
141
62.534
−41.718
8.444
1.00
19.26
A
C


ATOM
1097
O
LEU
A
141
61.665
−40.862
8.277
1.00
17.44
A
O


ATOM
1098
N
SER
A
142
62.909
−42.587
7.511
1.00
18.27
A
N


ATOM
1099
CA
SER
A
142
62.341
−42.594
6.206
1.00
20.46
A
C


ATOM
1100
CB
SER
A
142
63.124
−43.548
5.321
1.00
26.53
A
C


ATOM
1101
OG
SER
A
142
62.814
−43.271
3.988
1.00
46.16
A
O


ATOM
1102
C
SER
A
142
60.847
−42.966
6.271
1.00
18.21
A
C


ATOM
1103
O
SER
A
142
60.037
−42.395
5.534
1.00
14.69
A
O


ATOM
1104
N
LEU
A
143
60.488
−43.888
7.172
1.00
16.13
A
N


ATOM
1105
CA
LEU
A
143
59.100
−44.268
7.381
1.00
17.04
A
C


ATOM
1106
CB
LEU
A
143
59.006
−45.525
8.279
1.00
15.69
A
C


ATOM
1107
CG
LEU
A
143
57.615
−46.033
8.546
1.00
14.71
A
C


ATOM
1108
CD1
LEU
A
143
57.003
−46.535
7.244
1.00
16.08
A
C


ATOM
1109
CD2
LEU
A
143
57.657
−47.149
9.631
1.00
15.44
A
C


ATOM
1110
C
LEU
A
143
58.311
−43.097
7.984
1.00
17.25
A
C


ATOM
1111
O
LEU
A
143
57.207
−42.802
7.561
1.00
15.03
A
O


ATOM
1112
N
TYR
A
144
58.912
−42.395
8.934
1.00
15.40
A
N


ATOM
1113
CA
TYR
A
144
58.325
−41.187
9.491
1.00
16.10
A
C


ATOM
1114
CB
TYR
A
144
59.225
−40.646
10.613
1.00
15.60
A
C


ATOM
1115
CG
TYR
A
144
58.902
−39.282
11.170
1.00
16.31
A
C


ATOM
1116
CD1
TYR
A
144
57.977
−39.090
12.193
1.00
16.83
A
C


ATOM
1117
CE1
TYR
A
144
57.700
−37.787
12.674
1.00
19.48
A
C


ATOM
1118
CZ
TYR
A
144
58.351
−36.728
12.126
1.00
19.49
A
C


ATOM
1119
OH
TYR
A
144
58.165
−35.414
12.516
1.00
33.50
A
O


ATOM
1120
CE2
TYR
A
144
59.229
−36.910
11.105
1.00
23.79
A
C


ATOM
1121
CD2
TYR
A
144
59.496
−38.164
10.630
1.00
22.71
A
C


ATOM
1122
C
TYR
A
144
58.059
−40.146
8.400
1.00
16.74
A
C


ATOM
1123
O
TYR
A
144
56.955
−39.586
8.314
1.00
14.47
A
O


ATOM
1124
N
GLU
A
145
59.041
−39.909
7.530
1.00
15.21
A
N


ATOM
1125
CA
GLU
A
145
58.873
−38.862
6.509
1.00
15.72
A
C


ATOM
1126
CB
GLU
A
145
60.154
−38.637
5.722
1.00
18.15
A
C


ATOM
1127
CG
GLU
A
145
61.344
−38.139
6.595
1.00
19.87
A
C


ATOM
1128
CD
GLU
A
145
61.188
−36.679
7.108
1.00
24.51
A
C


ATOM
1129
OE1
GLU
A
145
60.171
−36.021
6.801
1.00
29.03
A
O


ATOM
1130
OE2
GLU
A
145
62.097
−36.173
7.820
1.00
28.27
A
O


ATOM
1131
C
GLU
A
145
57.738
−39.213
5.541
1.00
16.62
A
C


ATOM
1132
O
GLU
A
145
56.952
−38.359
5.182
1.00
15.05
A
O


ATOM
1133
N
ALA
A
146
57.690
−40.475
5.134
1.00
13.68
A
N


ATOM
1134
CA
ALA
A
146
56.692
−40.980
4.200
1.00
15.91
A
C


ATOM
1135
CB
ALA
A
146
56.968
−42.430
3.894
1.00
12.43
A
C


ATOM
1136
C
ALA
A
146
55.265
−40.849
4.755
1.00
15.88
A
C


ATOM
1137
O
ALA
A
146
54.344
−40.597
4.002
1.00
16.60
A
O


ATOM
1138
N
SER
A
147
55.099
−41.014
6.068
1.00
14.09
A
N


ATOM
1139
CA
SER
A
147
53.764
−41.019
6.688
1.00
14.89
A
C


ATOM
1140
CB
SER
A
147
53.879
−41.395
8.172
1.00
15.06
A
C


ATOM
1141
OG
SER
A
147
54.428
−40.319
8.928
1.00
18.70
A
O


ATOM
1142
C
SER
A
147
53.002
−39.717
6.510
1.00
17.14
A
C


ATOM
1143
O
SER
A
147
51.777
−39.709
6.490
1.00
17.46
A
O


ATOM
1144
N
PHE
A
148
53.726
−38.612
6.309
1.00
15.93
A
N


ATOM
1145
CA
PHE
A
148
53.088
−37.334
6.131
1.00
15.22
A
C


ATOM
1146
CB
PHE
A
148
54.047
−36.189
6.491
1.00
16.39
A
C


ATOM
1147
CG
PHE
A
148
54.337
−36.123
7.963
1.00
15.45
A
C


ATOM
1148
CD1
PHE
A
148
53.478
−35.439
8.819
1.00
17.42
A
C


ATOM
1149
CE1
PHE
A
148
53.725
−35.385
10.197
1.00
19.59
A
C


ATOM
1150
CZ
PHE
A
148
54.841
−36.077
10.727
1.00
19.38
A
C


ATOM
1151
CE2
PHE
A
148
55.653
−36.791
9.881
1.00
18.32
A
C


ATOM
1152
CD2
PHE
A
148
55.405
−36.793
8.492
1.00
17.53
A
C


ATOM
1153
C
PHE
A
148
52.471
−37.155
4.762
1.00
15.54
A
C


ATOM
1154
O
PHE
A
148
51.778
−36.169
4.563
1.00
15.38
A
O


ATOM
1155
N
LEU
A
149
52.730
−38.080
3.830
1.00
15.31
A
N


ATOM
1156
CA
LEU
A
149
52.150
−37.987
2.482
1.00
18.47
A
C


ATOM
1157
CB
LEU
A
149
53.157
−38.474
1.415
1.00
18.22
A
C


ATOM
1158
CG
LEU
A
149
54.234
−37.445
1.047
1.00
21.82
A
C


ATOM
1159
CD1
LEU
A
149
55.387
−37.390
2.130
1.00
19.29
A
C


ATOM
1160
CD2
LEU
A
149
54.819
−37.691
−0.339
1.00
22.36
A
C


ATOM
1161
C
LEU
A
149
50.836
−38.752
2.370
1.00
16.60
A
C


ATOM
1162
O
LEU
A
149
50.307
−38.944
1.252
1.00
15.30
A
O


ATOM
1163
N
ALA
A
150
50.317
−39.173
3.517
1.00
15.81
A
N


ATOM
1164
CA
ALA
A
150
49.107
−39.941
3.618
1.00
17.34
A
C


ATOM
1165
CB
ALA
A
150
48.823
−40.301
5.087
1.00
15.45
A
C


ATOM
1166
C
ALA
A
150
47.881
−39.234
3.050
1.00
18.40
A
C


ATOM
1167
O
ALA
A
150
47.712
−38.025
3.186
1.00
16.98
A
O


ATOM
1168
N
LEU
A
151
46.981
−40.043
2.535
1.00
14.75
A
N


ATOM
1169
CA
LEU
A
151
45.632
−39.599
2.206
1.00
16.19
A
C


ATOM
1170
CB
LEU
A
151
45.243
−40.145
0.854
1.00
16.19
A
C


ATOM
1171
CG
LEU
A
151
46.247
−40.004
−0.282
1.00
19.06
A
C


ATOM
1172
CD1
LEU
A
151
45.756
−40.681
−1.566
1.00
22.57
A
C


ATOM
1173
CD2
LEU
A
151
46.541
−38.559
−0.537
1.00
16.77
A
C


ATOM
1174
C
LEU
A
151
44.652
−40.073
3.254
1.00
13.22
A
C


ATOM
1175
O
LEU
A
151
44.974
−40.943
4.082
1.00
14.48
A
O


ATOM
1176
N
GLU
A
152
43.417
−39.593
3.181
1.00
14.18
A
N


ATOM
1177
CA
GLU
A
152
42.394
−40.062
4.089
1.00
14.14
A
C


ATOM
1178
CB
GLU
A
152
41.085
−39.287
3.961
1.00
18.51
A
C


ATOM
1179
CG
GLU
A
152
41.129
−37.846
4.564
1.00
23.96
A
C


ATOM
1180
CD
GLU
A
152
39.734
−37.206
4.652
1.00
32.90
A
C


ATOM
1181
OE1
GLU
A
152
39.168
−36.845
3.585
1.00
28.65
A
O


ATOM
1182
OE2
GLU
A
152
39.196
−37.096
5.785
1.00
31.52
A
O


ATOM
1183
C
GLU
A
152
42.172
−41.546
3.898
1.00
17.44
A
C


ATOM
1184
O
GLU
A
152
42.205
−42.036
2.782
1.00
14.59
A
O


ATOM
1185
N
GLY
A
153
41.970
−42.249
5.014
1.00
15.78
A
N


ATOM
1186
CA
GLY
A
153
41.818
−43.699
5.015
1.00
16.99
A
C


ATOM
1187
C
GLY
A
153
43.081
−44.544
4.948
1.00
14.91
A
C


ATOM
1188
O
GLY
A
153
42.992
−45.752
5.026
1.00
13.14
A
O


ATOM
1189
N
GLU
A
154
44.258
−43.937
4.852
1.00
13.94
A
N


ATOM
1190
CA
GLU
A
154
45.497
−44.703
4.823
1.00
16.14
A
C


ATOM
1191
CB
GLU
A
154
46.542
−44.018
3.943
1.00
16.91
A
C


ATOM
1192
CG
GLU
A
154
46.197
−44.125
2.469
1.00
18.41
A
C


ATOM
1193
CD
GLU
A
154
47.257
−43.609
1.503
1.00
23.40
A
C


ATOM
1194
OE1
GLU
A
154
47.955
−42.590
1.798
1.00
17.72
A
O


ATOM
1195
OE2
GLU
A
154
47.356
−44.194
0.395
1.00
19.66
A
O


ATOM
1196
C
GLU
A
154
45.975
−44.935
6.269
1.00
17.71
A
C


ATOM
1197
O
GLU
A
154
46.989
−44.389
6.726
1.00
14.88
A
O


ATOM
1198
N
ASN
A
155
45.247
−45.807
6.950
1.00
16.60
A
N


ATOM
1199
CA
ASN
A
155
45.489
−46.075
8.350
1.00
18.90
A
C


ATOM
1200
CB
ASN
A
155
44.434
−47.017
8.918
1.00
20.93
A
C


ATOM
1201
CG
ASN
A
155
44.443
−48.381
8.234
1.00
18.14
A
C


ATOM
1202
OD1
ASN
A
155
44.241
−48.483
7.024
1.00
16.09
A
O


ATOM
1203
ND2
ASN
A
155
44.679
−49.435
9.014
1.00
18.65
A
N


ATOM
1204
C
ASN
A
155
46.871
−46.650
8.630
1.00
20.12
A
C


ATOM
1205
O
ASN
A
155
47.401
−46.426
9.701
1.00
18.62
A
O


ATOM
1206
N
ILE
A
156
47.436
−47.399
7.682
1.00
16.20
A
N


ATOM
1207
CA
ILE
A
156
48.771
−47.949
7.861
1.00
16.77
A
C


ATOM
1208
CB
ILE
A
156
49.116
−48.931
6.754
1.00
18.33
A
C


ATOM
1209
CG1
ILE
A
156
48.132
−50.099
6.778
1.00
24.37
A
C


ATOM
1210
CD1
ILE
A
156
48.422
−51.119
5.712
1.00
29.91
A
C


ATOM
1211
CG2
ILE
A
156
50.550
−49.458
6.900
1.00
21.14
A
C


ATOM
1212
C
ILE
A
156
49.817
−46.837
7.948
1.00
19.06
A
C


ATOM
1213
O
ILE
A
156
50.766
−46.951
8.708
1.00
16.97
A
O


ATOM
1214
N
LEU
A
157
49.662
−45.765
7.172
1.00
17.32
A
N


ATOM
1215
CA
ALEU
A
157
50.602
−44.648
7.251
0.50
17.81
A
C


ATOM
1216
CA
BLEU
A
157
50.604
−44.657
7.252
0.50
18.60
A
C


ATOM
1217
CB
ALEU
A
157
50.464
−43.694
6.062
0.50
16.56
A
C


ATOM
1218
CB
BLEU
A
157
50.473
−43.735
6.042
0.50
18.39
A
C


ATOM
1219
CG
ALEU
A
157
51.257
−44.012
4.793
0.50
16.00
A
C


ATOM
1220
CG
BLEU
A
157
50.771
−44.416
4.705
0.50
18.36
A
C


ATOM
1221
CD1
ALEU
A
157
50.915
−45.378
4.215
0.50
14.54
A
C


ATOM
1222
CD1
BLEU
A
157
50.536
−43.465
3.509
0.50
19.37
A
C


ATOM
1223
CD2
ALEU
A
157
51.039
−42.915
3.747
0.50
14.62
A
C


ATOM
1224
CD2
BLEU
A
157
52.212
−44.943
4.712
0.50
22.52
A
C


ATOM
1225
C
LEU
A
157
50.439
−43.913
8.583
1.00
20.05
A
C


ATOM
1226
O
LEU
A
157
51.428
−43.499
9.195
1.00
17.15
A
O


ATOM
1227
N
ASP
A
158
49.207
−43.763
9.055
1.00
16.45
A
N


ATOM
1228
CA
ASP
A
158
48.995
−43.157
10.381
1.00
18.02
A
C


ATOM
1229
CB
ASP
A
158
47.495
−42.962
10.675
1.00
23.70
A
C


ATOM
1230
CG
ASP
A
158
46.899
−41.736
9.945
1.00
36.75
A
C


ATOM
1231
OD1
ASP
A
158
47.640
−41.003
9.246
1.00
46.65
A
O


ATOM
1232
OD2
ASP
A
158
45.680
−41.496
10.074
1.00
30.74
A
O


ATOM
1233
C
ASP
A
158
49.647
−43.996
11.493
1.00
21.85
A
C


ATOM
1234
O
ASP
A
158
50.281
−43.456
12.407
1.00
17.84
A
O


ATOM
1235
N
GLU
A
159
49.481
−45.306
11.415
1.00
17.82
A
N


ATOM
1236
CA
GLU
A
159
50.149
−46.251
12.356
1.00
22.88
A
C


ATOM
1237
CB
GLU
A
159
49.644
−47.683
12.101
1.00
21.78
A
C


ATOM
1238
CG
GLU
A
159
48.159
−47.853
12.486
1.00
27.20
A
C


ATOM
1239
CD
GLU
A
159
47.455
−49.026
11.768
1.00
33.26
A
C


ATOM
1240
OE1
GLU
A
159
48.161
−49.941
11.281
1.00
33.28
A
O


ATOM
1241
OE2
GLU
A
159
46.196
−49.014
11.697
1.00
41.36
A
O


ATOM
1242
C
GLU
A
159
51.682
−46.221
12.217
1.00
21.29
A
C


ATOM
1243
O
GLU
A
159
52.395
−46.328
13.204
1.00
18.19
A
O


ATOM
1244
N
ALA
A
160
52.170
−46.067
10.982
1.00
18.94
A
N


ATOM
1245
CA
ALA
A
160
53.604
−45.903
10.733
1.00
20.13
A
C


ATOM
1246
CB
ALA
A
160
53.902
−45.809
9.257
1.00
18.72
A
C


ATOM
1247
C
ALA
A
160
54.142
−44.682
11.465
1.00
18.25
A
C


ATOM
1248
O
ALA
A
160
55.204
−44.741
12.055
1.00
20.17
A
O


ATOM
1249
N
LYS
A
161
53.422
−43.569
11.413
1.00
19.03
A
N


ATOM
1250
CA
LYS
A
161
53.870
−42.372
12.079
1.00
16.85
A
C


ATOM
1251
CB
LYS
A
161
52.987
−41.176
11.756
1.00
17.64
A
C


ATOM
1252
CG
LYS
A
161
53.497
−39.838
12.266
1.00
19.23
A
C


ATOM
1253
CD
LYS
A
161
52.393
−38.752
12.235
1.00
21.55
A
C


ATOM
1254
CE
LYS
A
161
51.991
−38.399
10.792
1.00
22.08
A
C


ATOM
1255
NZ
LYS
A
161
50.873
−37.403
10.754
1.00
28.22
A
N


ATOM
1256
C
LYS
A
161
53.971
−42.615
13.600
1.00
19.00
A
C


ATOM
1257
O
LYS
A
161
54.974
−42.238
14.208
1.00
21.46
A
O


ATOM
1258
N
VAL
A
162
52.955
−43.233
14.196
1.00
21.78
A
N


ATOM
1259
CA
VAL
A
162
52.939
−43.495
15.637
1.00
21.10
A
C


ATOM
1260
CB
VAL
A
162
51.618
−44.152
16.075
1.00
25.19
A
C


ATOM
1261
CG1
VAL
A
162
51.737
−44.735
17.525
1.00
21.82
A
C


ATOM
1262
CG2
VAL
A
162
50.480
−43.145
15.953
1.00
20.91
A
C


ATOM
1263
C
VAL
A
162
54.128
−44.369
16.042
1.00
22.12
A
C


ATOM
1264
O
VAL
A
162
54.817
−44.096
17.018
1.00
20.45
A
O


ATOM
1265
N
PHE
A
163
54.366
−45.415
15.278
1.00
21.27
A
N


ATOM
1266
CA
PHE
A
163
55.483
−46.320
15.521
1.00
23.48
A
C


ATOM
1267
CB
PHE
A
163
55.327
−47.472
14.531
1.00
20.49
A
C


ATOM
1268
CG
PHE
A
163
56.455
−48.422
14.504
1.00
21.07
A
C


ATOM
1269
CD1
PHE
A
163
56.538
−49.457
15.445
1.00
23.36
A
C


ATOM
1270
CE1
PHE
A
163
57.573
−50.363
15.398
1.00
22.92
A
C


ATOM
1271
CZ
PHE
A
163
58.538
−50.251
14.390
1.00
23.85
A
C


ATOM
1272
CE2
PHE
A
163
58.440
−49.248
13.430
1.00
24.53
A
C


ATOM
1273
CD2
PHE
A
163
57.401
−48.344
13.492
1.00
21.66
A
C


ATOM
1274
C
PHE
A
163
56.857
−45.607
15.401
1.00
23.17
A
C


ATOM
1275
O
PHE
A
163
57.719
−45.684
16.281
1.00
20.56
A
O


ATOM
1276
N
ALA
A
164
57.037
−44.867
14.322
1.00
19.68
A
N


ATOM
1277
CA
ALA
A
164
58.311
−44.248
14.037
1.00
18.42
A
C


ATOM
1278
CB
ALA
A
164
58.285
−43.579
12.677
1.00
13.78
A
C


ATOM
1279
C
ALA
A
164
58.636
−43.240
15.139
1.00
21.21
A
C


ATOM
1280
O
ALA
A
164
59.723
−43.286
15.723
1.00
22.03
A
O


ATOM
1281
N
ILE
A
165
57.682
−42.371
15.446
1.00
21.64
A
N


ATOM
1282
CA
AILE
A
165
57.833
−41.339
16.473
0.50
20.95
A
C


ATOM
1283
CA
BILE
A
165
57.909
−41.334
16.442
0.50
23.71
A
C


ATOM
1284
CB
AILE
A
165
56.517
−40.537
16.650
0.50
20.64
A
C


ATOM
1285
CB
BILE
A
165
56.732
−40.324
16.516
0.50
27.11
A
C


ATOM
1286
CG1
AILE
A
165
56.443
−39.411
15.618
0.50
21.15
A
C


ATOM
1287
CG1
BILE
A
165
57.136
−39.071
17.301
0.50
31.60
A
C


ATOM
1288
CD1
AILE
A
165
55.073
−38.748
15.482
0.50
18.04
A
C


ATOM
1289
CD1
BILE
A
165
55.959
−38.281
17.848
0.50
32.38
A
C


ATOM
1290
CG2
AILE
A
165
56.392
−40.001
18.089
0.50
21.87
A
C


ATOM
1291
CG2
BILE
A
165
55.525
−40.956
17.150
0.50
36.27
A
C


ATOM
1292
C
ILE
A
165
58.224
−41.952
17.815
1.00
24.18
A
C


ATOM
1293
O
ILE
A
165
59.101
−41.458
18.516
1.00
23.80
A
O


ATOM
1294
N
SER
A
166
57.560
−43.046
18.165
1.00
24.66
A
N


ATOM
1295
CA
SER
A
166
57.784
−43.709
19.445
1.00
25.26
A
C


ATOM
1296
CB
SER
A
166
56.838
−44.917
19.587
1.00
25.78
A
C


ATOM
1297
OG
SER
A
166
57.370
−46.095
18.941
1.00
29.45
A
O


ATOM
1298
C
SER
A
166
59.237
−44.173
19.578
1.00
26.55
A
C


ATOM
1299
O
SER
A
166
59.821
−44.112
20.648
1.00
30.51
A
O


ATOM
1300
N
HIS
A
167
59.807
−44.638
18.477
1.00
25.51
A
N


ATOM
1301
CA
HIS
A
167
61.160
−45.136
18.451
1.00
23.28
A
C


ATOM
1302
CB
HIS
A
167
61.278
−46.258
17.447
1.00
23.58
A
C


ATOM
1303
CG
HIS
A
167
60.569
−47.536
17.869
1.00
34.21
A
C


ATOM
1304
ND1
HIS
A
167
60.813
−48.150
19.048
1.00
31.37
A
N


ATOM
1305
CE1
HIS
A
167
60.052
−49.257
19.151
1.00
36.74
A
C


ATOM
1306
NE2
HIS
A
167
59.321
−49.364
18.032
1.00
40.73
A
N


ATOM
1307
CD2
HIS
A
167
59.604
−48.309
17.222
1.00
34.62
A
C


ATOM
1308
C
HIS
A
167
62.171
−44.054
18.194
1.00
29.12
A
C


ATOM
1309
O
HIS
A
167
63.315
−44.185
18.609
1.00
36.79
A
O


ATOM
1310
N
LEU
A
168
61.772
−42.951
17.561
1.00
22.31
A
N


ATOM
1311
CA
LEU
A
168
62.700
−41.854
17.326
1.00
21.97
A
C


ATOM
1312
CB
LEU
A
168
62.289
−41.008
16.130
1.00
24.55
A
C


ATOM
1313
CG
LEU
A
168
62.324
−41.692
14.762
1.00
23.28
A
C


ATOM
1314
CD1
LEU
A
168
61.510
−40.848
13.755
1.00
22.93
A
C


ATOM
1315
CD2
LEU
A
168
63.755
−41.916
14.281
1.00
20.77
A
C


ATOM
1316
C
LEU
A
168
62.839
−40.917
18.528
1.00
25.89
A
C


ATOM
1317
O
LEU
A
168
63.890
−40.328
18.689
1.00
23.33
A
O


ATOM
1318
N
LYS
A
169
61.770
−40.764
19.323
1.00
22.50
A
N


ATOM
1319
CA
LYS
A
169
61.679
−39.744
20.370
1.00
27.82
A
C


ATOM
1320
CB
LYS
A
169
60.250
−39.667
20.937
1.00
34.22
A
C


ATOM
1321
CG
LYS
A
169
59.734
−38.249
21.087
1.00
50.55
A
C


ATOM
1322
CD
LYS
A
169
58.226
−38.198
21.357
1.00
58.76
A
C


ATOM
1323
CE
LYS
A
169
57.572
−37.025
20.621
1.00
62.12
A
C


ATOM
1324
NZ
LYS
A
169
56.090
−37.061
20.706
1.00
59.64
A
N


ATOM
1325
C
LYS
A
169
62.623
−40.088
21.520
1.00
37.79
A
C


ATOM
1326
O
LYS
A
169
63.068
−39.208
22.255
1.00
38.87
A
O


ATOM
1327
N
GLU
A
170
62.915
−41.374
21.668
1.00
33.46
A
N


ATOM
1328
CA
GLU
A
170
63.722
−41.860
22.772
1.00
50.73
A
C


ATOM
1329
CB
GLU
A
170
63.038
−43.071
23.433
1.00
58.58
A
C


ATOM
1330
CG
GLU
A
170
62.410
−42.749
24.796
1.00
74.37
A
C


ATOM
1331
CD
GLU
A
170
60.954
−42.354
24.711
1.00
82.28
A
C


ATOM
1332
OE1
GLU
A
170
60.228
−42.912
23.857
1.00
101.69
A
O


ATOM
1333
OE2
GLU
A
170
60.532
−41.496
25.516
1.00
83.80
A
O


ATOM
1334
C
GLU
A
170
65.145
−42.221
22.362
1.00
53.62
A
C


ATOM
1335
O
GLU
A
170
65.836
−42.921
23.099
1.00
49.28
A
O


ATOM
1336
N
LEU
A
171
65.596
−41.737
21.208
1.00
46.07
A
N


ATOM
1337
CA
LEU
A
171
66.975
−41.966
20.799
1.00
47.42
A
C


ATOM
1338
CB
LEU
A
171
67.136
−41.777
19.294
1.00
39.33
A
C


ATOM
1339
CG
LEU
A
171
66.477
−42.875
18.462
1.00
38.86
A
C


ATOM
1340
CD1
LEU
A
171
66.646
−42.622
16.958
1.00
29.01
A
C


ATOM
1341
CD2
LEU
A
171
67.026
−44.265
18.848
1.00
41.31
A
C


ATOM
1342
C
LEU
A
171
67.929
−41.047
21.581
1.00
49.73
A
C


ATOM
1343
O
LEU
A
171
67.566
−39.919
21.935
1.00
42.22
A
O


ATOM
1344
N
SER
A
172
69.132
−41.559
21.852
1.00
55.46
A
N


ATOM
1345
CA
SER
A
172
70.192
−40.818
22.558
1.00
59.52
A
C


ATOM
1346
CB
SER
A
172
70.665
−41.614
23.788
1.00
67.88
A
C


ATOM
1347
OG
SER
A
172
71.656
−40.922
24.534
1.00
70.11
A
O


ATOM
1348
C
SER
A
172
71.370
−40.566
21.607
1.00
47.16
A
C


ATOM
1349
O
SER
A
172
71.831
−41.484
20.918
1.00
38.06
A
O


ATOM
1350
N
GLU
A
173
71.846
−39.324
21.572
1.00
44.33
A
N


ATOM
1351
CA
GLU
A
173
72.989
−38.930
20.733
1.00
48.18
A
C


ATOM
1352
CB
GLU
A
173
73.371
−37.480
21.048
1.00
51.55
A
C


ATOM
1353
CG
GLU
A
173
74.686
−37.024
20.417
1.00
54.88
A
C


ATOM
1354
CD
GLU
A
173
74.730
−35.532
20.179
1.00
65.93
A
C


ATOM
1355
OE1
GLU
A
173
73.946
−34.790
20.812
1.00
65.86
A
O


ATOM
1356
OE2
GLU
A
173
75.552
−35.103
19.344
1.00
83.90
A
O


ATOM
1357
C
GLU
A
173
74.236
−39.827
20.868
1.00
49.00
A
C


ATOM
1358
O
GLU
A
173
74.931
−40.082
19.870
1.00
37.80
A
O


ATOM
1359
N
GLU
A
174
74.515
−40.280
22.096
1.00
48.12
A
N


ATOM
1360
CA
GLU
A
174
75.612
−41.220
22.363
1.00
55.68
A
C


ATOM
1361
CB
GLU
A
174
75.771
−41.484
23.865
1.00
58.22
A
C


ATOM
1362
CG
GLU
A
174
76.324
−40.317
24.665
1.00
69.42
A
C


ATOM
1363
CD
GLU
A
174
75.241
−39.438
25.253
1.00
76.47
A
C


ATOM
1364
OE1
GLU
A
174
74.227
−39.181
24.565
1.00
73.99
A
O


ATOM
1365
OE2
GLU
A
174
75.408
−39.001
26.410
1.00
85.80
A
O


ATOM
1366
C
GLU
A
174
75.392
−42.552
21.660
1.00
57.03
A
C


ATOM
1367
O
GLU
A
174
76.314
−43.095
21.047
1.00
61.24
A
O


ATOM
1368
N
LYS
A
175
74.175
−43.079
21.763
1.00
57.03
A
N


ATOM
1369
CA
LYS
A
175
73.825
−44.340
21.102
1.00
64.40
A
C


ATOM
1370
CB
LYS
A
175
72.351
−44.714
21.353
1.00
84.22
A
C


ATOM
1371
CG
LYS
A
175
71.929
−44.855
22.825
1.00
94.98
A
C


ATOM
1372
CD
LYS
A
175
71.932
−46.299
23.316
1.00
97.31
A
C


ATOM
1373
CE
LYS
A
175
71.585
−46.374
24.798
1.00
88.82
A
C


ATOM
1374
NZ
LYS
A
175
71.600
−47.764
25.317
1.00
78.69
A
N


ATOM
1375
C
LYS
A
175
74.066
−44.248
19.591
1.00
61.10
A
C


ATOM
1376
O
LYS
A
175
74.760
−45.094
19.018
1.00
53.35
A
O


ATOM
1377
N
ILE
A
176
73.517
−43.208
18.954
1.00
42.18
A
N


ATOM
1378
CA
ILE
A
176
73.412
−43.188
17.487
1.00
41.76
A
C


ATOM
1379
CB
ILE
A
176
71.963
−42.835
17.019
1.00
39.25
A
C


ATOM
1380
CG1
ILE
A
176
71.533
−41.438
17.477
1.00
35.80
A
C


ATOM
1381
CD1
ILE
A
176
70.242
−40.971
16.809
1.00
37.64
A
C


ATOM
1382
CG2
ILE
A
176
70.983
−43.882
17.535
1.00
45.13
A
C


ATOM
1383
C
ILE
A
176
74.423
−42.324
16.734
1.00
37.07
A
C


ATOM
1384
O
ILE
A
176
74.591
−42.498
15.520
1.00
35.40
A
O


ATOM
1385
N
GLY
A
177
75.067
−41.391
17.429
1.00
35.06
A
N


ATOM
1386
CA
GLY
A
177
76.065
−40.512
16.812
1.00
33.17
A
C


ATOM
1387
C
GLY
A
177
75.500
−39.146
16.501
1.00
38.96
A
C


ATOM
1388
O
GLY
A
177
74.287
−38.986
16.355
1.00
31.32
A
O


ATOM
1389
N
LYS
A
178
76.389
−38.163
16.383
1.00
35.12
A
N


ATOM
1390
CA
LYS
A
178
75.995
−36.761
16.293
1.00
35.31
A
C


ATOM
1391
CB
LYS
A
178
77.215
−35.842
16.372
1.00
39.53
A
C


ATOM
1392
CG
LYS
A
178
76.882
−34.354
16.392
1.00
46.00
A
C


ATOM
1393
CD
LYS
A
178
78.060
−33.521
16.888
1.00
58.26
A
C


ATOM
1394
CE
LYS
A
178
77.714
−32.034
16.993
1.00
68.89
A
C


ATOM
1395
NZ
LYS
A
178
78.519
−31.320
18.047
1.00
71.94
A
N


ATOM
1396
C
LYS
A
178
75.207
−36.428
15.030
1.00
36.95
A
C


ATOM
1397
O
LYS
A
178
74.277
−35.624
15.086
1.00
31.16
A
O


ATOM
1398
N
GLU
A
179
75.578
−37.024
13.901
1.00
31.99
A
N


ATOM
1399
CA
GLU
A
179
74.988
−36.622
12.636
1.00
42.03
A
C


ATOM
1400
CB
GLU
A
179
75.912
−36.968
11.452
1.00
43.93
A
C


ATOM
1401
CG
GLU
A
179
75.578
−38.196
10.631
1.00
49.30
A
C


ATOM
1402
CD
GLU
A
179
76.495
−38.340
9.410
1.00
62.45
A
C


ATOM
1403
OE1
GLU
A
179
77.641
−37.830
9.436
1.00
60.91
A
O


ATOM
1404
OE2
GLU
A
179
76.069
−38.961
8.416
1.00
60.25
A
O


ATOM
1405
C
GLU
A
179
73.573
−37.211
12.520
1.00
44.22
A
C


ATOM
1406
O
GLU
A
179
72.636
−36.510
12.145
1.00
38.74
A
O


ATOM
1407
N
LEU
A
180
73.427
−38.485
12.883
1.00
32.17
A
N


ATOM
1408
CA
LEU
A
180
72.130
−39.120
12.907
1.00
37.01
A
C


ATOM
1409
CB
LEU
A
180
72.291
−40.623
13.123
1.00
36.21
A
C


ATOM
1410
CG
LEU
A
180
71.215
−41.550
12.596
1.00
39.58
A
C


ATOM
1411
CD1
LEU
A
180
70.761
−41.185
11.164
1.00
39.66
A
C


ATOM
1412
CD2
LEU
A
180
71.758
−42.977
12.663
1.00
43.88
A
C


ATOM
1413
C
LEU
A
180
71.218
−38.457
13.958
1.00
33.76
A
C


ATOM
1414
O
LEU
A
180
70.047
−38.205
13.694
1.00
27.31
A
O


ATOM
1415
N
ALA
A
181
71.749
−38.114
15.121
1.00
29.65
A
N


ATOM
1416
CA
ALA
A
181
70.945
−37.388
16.116
1.00
28.86
A
C


ATOM
1417
CB
ALA
A
181
71.716
−37.212
17.394
1.00
25.84
A
C


ATOM
1418
C
ALA
A
181
70.455
−36.028
15.597
1.00
31.37
A
C


ATOM
1419
O
ALA
A
181
69.320
−35.584
15.893
1.00
25.03
A
O


ATOM
1420
N
GLU
A
182
71.304
−35.359
14.825
1.00
25.42
A
N


ATOM
1421
CA
GLU
A
182
70.911
−34.077
14.240
1.00
27.62
A
C


ATOM
1422
CB
GLU
A
182
72.106
−33.365
13.594
1.00
33.59
A
C


ATOM
1423
CG
GLU
A
182
72.999
−32.617
14.607
1.00
53.21
A
C


ATOM
1424
CD
GLU
A
182
74.282
−32.037
13.996
1.00
74.57
A
C


ATOM
1425
OE1
GLU
A
182
74.532
−32.231
12.783
1.00
106.13
A
O


ATOM
1426
OE2
GLU
A
182
75.042
−31.380
14.739
1.00
82.10
A
O


ATOM
1427
C
GLU
A
182
69.785
−34.274
13.209
1.00
19.86
A
C


ATOM
1428
O
GLU
A
182
68.862
−33.494
13.151
1.00
18.27
A
O


ATOM
1429
N
GLN
A
183
69.925
−35.293
12.377
1.00
20.24
A
N


ATOM
1430
CA
AGLN
A
183
68.906
−35.650
11.404
0.50
24.85
A
C


ATOM
1431
CA
BGLN
A
183
68.898
−35.606
11.398
0.50
21.35
A
C


ATOM
1432
CB
AGLN
A
183
69.366
−36.880
10.614
0.50
29.98
A
C


ATOM
1433
CB
BGLN
A
183
69.355
−36.749
10.483
0.50
20.81
A
C


ATOM
1434
CG
AGLN
A
183
68.795
−36.970
9.221
0.50
37.32
A
C


ATOM
1435
CG
BGLN
A
183
70.472
−36.372
9.497
0.50
19.34
A
C


ATOM
1436
CD
AGLN
A
183
69.349
−35.886
8.313
0.50
46.36
A
C


ATOM
1437
CD
BGLN
A
183
69.946
−35.727
8.205
0.50
24.20
A
C


ATOM
1438
OE1
AGLN
A
183
68.603
−35.049
7.809
0.50
48.56
A
O


ATOM
1439
OE1
BGLN
A
183
68.756
−35.827
7.885
0.50
23.40
A
O


ATOM
1440
NE2
AGLN
A
183
70.666
−35.889
8.115
0.50
45.01
A
N


ATOM
1441
NE2
BGLN
A
183
70.836
−35.076
7.456
0.50
23.18
A
N


ATOM
1442
C
GLN
A
183
67.589
−35.942
12.138
1.00
22.31
A
C


ATOM
1443
O
GLN
A
183
66.529
−35.497
11.740
1.00
20.24
A
O


ATOM
1444
N
VAL
A
184
67.668
−36.693
13.242
1.00
18.60
A
N


ATOM
1445
CA
VAL
A
184
66.447
−37.108
13.963
1.00
21.87
A
C


ATOM
1446
CB
VAL
A
184
66.738
−38.177
15.046
1.00
21.28
A
C


ATOM
1447
CG1
VAL
A
184
65.593
−38.311
16.034
1.00
22.68
A
C


ATOM
1448
CG2
VAL
A
184
67.040
−39.504
14.410
1.00
25.19
A
C


ATOM
1449
C
VAL
A
184
65.769
−35.888
14.567
1.00
20.78
A
C


ATOM
1450
O
VAL
A
184
64.541
−35.696
14.458
1.00
16.58
A
O


ATOM
1451
N
ASN
A
185
66.563
−35.018
15.159
1.00
20.29
A
N


ATOM
1452
CA
ASN
A
185
65.995
−33.828
15.770
1.00
22.74
A
C


ATOM
1453
CB
ASN
A
185
67.032
−33.166
16.676
1.00
25.83
A
C


ATOM
1454
CG
ASN
A
185
67.369
−34.038
17.885
1.00
32.89
A
C


ATOM
1455
OD1
ASN
A
185
66.486
−34.683
18.462
1.00
35.58
A
O


ATOM
1456
ND2
ASN
A
185
68.638
−34.061
18.273
1.00
30.79
A
N


ATOM
1457
C
ASN
A
185
65.385
−32.857
14.764
1.00
19.89
A
C


ATOM
1458
O
ASN
A
185
64.363
−32.215
15.022
1.00
18.89
A
O


ATOM
1459
N
HIS
A
186
65.986
−32.748
13.604
1.00
16.79
A
N


ATOM
1460
CA
HIS
A
186
65.440
−31.895
12.565
1.00
18.43
A
C


ATOM
1461
CB
HIS
A
186
66.481
−31.869
11.432
1.00
19.47
A
C


ATOM
1462
CG
HIS
A
186
66.096
−31.083
10.223
1.00
17.10
A
C


ATOM
1463
ND1
HIS
A
186
66.082
−29.746
10.200
1.00
18.23
A
N


ATOM
1464
CE1
HIS
A
186
65.754
−29.318
8.970
1.00
19.21
A
C


ATOM
1465
NE2
HIS
A
186
65.519
−30.394
8.197
1.00
21.37
A
N


ATOM
1466
CD2
HIS
A
186
65.735
−31.506
8.930
1.00
18.75
A
C


ATOM
1467
C
HIS
A
186
64.074
−32.454
12.118
1.00
17.24
A
C


ATOM
1468
O
HIS
A
186
63.100
−31.701
11.979
1.00
18.68
A
O


ATOM
1469
N
ALA
A
187
64.009
−33.752
11.860
1.00
22.68
A
N


ATOM
1470
CA
ALA
A
187
62.779
−34.390
11.372
1.00
24.59
A
C


ATOM
1471
CB
ALA
A
187
63.058
−35.842
11.031
1.00
21.99
A
C


ATOM
1472
C
ALA
A
187
61.667
−34.277
12.434
1.00
22.96
A
C


ATOM
1473
O
ALA
A
187
60.504
−34.046
12.109
1.00
18.69
A
O


ATOM
1474
N
LEU
A
188
62.012
−34.390
13.709
1.00
21.42
A
N


ATOM
1475
CA
LEU
A
188
60.982
−34.344
14.756
1.00
22.89
A
C


ATOM
1476
CB
LEU
A
188
61.480
−34.957
16.080
1.00
23.79
A
C


ATOM
1477
CG
LEU
A
188
61.646
−36.475
16.124
1.00
22.25
A
C


ATOM
1478
CD1
LEU
A
188
62.268
−36.936
17.462
1.00
22.43
A
C


ATOM
1479
CD2
LEU
A
188
60.344
−37.182
15.918
1.00
20.82
A
C


ATOM
1480
C
LEU
A
188
60.512
−32.920
14.962
1.00
24.71
A
C


ATOM
1481
O
LEU
A
188
59.382
−32.685
15.358
1.00
24.58
A
O


ATOM
1482
N
GLU
A
189
61.360
−31.948
14.648
1.00
23.21
A
N


ATOM
1483
CA
GLU
A
189
60.919
−30.562
14.682
1.00
21.78
A
C


ATOM
1484
CB
GLU
A
189
62.129
−29.639
14.410
1.00
22.25
A
C


ATOM
1485
CG
GLU
A
189
61.841
−28.180
14.309
1.00
27.16
A
C


ATOM
1486
CD
GLU
A
189
63.068
−27.386
13.845
1.00
38.35
A
C


ATOM
1487
OE1
GLU
A
189
63.522
−27.587
12.707
1.00
28.82
A
O


ATOM
1488
OE2
GLU
A
189
63.586
−26.565
14.625
1.00
35.95
A
O


ATOM
1489
C
GLU
A
189
59.823
−30.339
13.653
1.00
22.39
A
C


ATOM
1490
O
GLU
A
189
58.838
−29.674
13.905
1.00
22.62
A
O


ATOM
1491
N
LEU
A
190
60.042
−30.830
12.448
1.00
20.61
A
N


ATOM
1492
CA
LEU
A
190
59.068
−30.652
11.378
1.00
21.06
A
C


ATOM
1493
CB
LEU
A
190
59.029
−29.194
10.897
1.00
22.31
A
C


ATOM
1494
CG
LEU
A
190
57.874
−28.842
9.980
1.00
24.65
A
C


ATOM
1495
CD1
LEU
A
190
56.530
−28.855
10.791
1.00
21.42
A
C


ATOM
1496
CD2
LEU
A
190
58.138
−27.521
9.235
1.00
22.03
A
C


ATOM
1497
C
LEU
A
190
59.506
−31.576
10.246
1.00
22.03
A
C


ATOM
1498
O
LEU
A
190
60.673
−31.522
9.828
1.00
18.81
A
O


ATOM
1499
N
PRO
A
191
58.591
−32.426
9.756
1.00
18.19
A
N


ATOM
1500
CA
PRO
A
191
58.901
−33.331
8.653
1.00
19.53
A
C


ATOM
1501
CB
PRO
A
191
57.674
−34.218
8.597
1.00
17.31
A
C


ATOM
1502
CG
PRO
A
191
56.584
−33.313
8.960
1.00
19.81
A
C


ATOM
1503
CD
PRO
A
191
57.155
−32.489
10.095
1.00
18.14
A
C


ATOM
1504
C
PRO
A
191
59.152
−32.627
7.314
1.00
20.66
A
C


ATOM
1505
O
PRO
A
191
58.700
−31.510
7.102
1.00
18.92
A
O


ATOM
1506
N
LEU
A
192
59.912
−33.291
6.446
1.00
17.57
A
N


ATOM
1507
CA
LEU
A
192
60.286
−32.789
5.145
1.00
20.14
A
C


ATOM
1508
CB
LEU
A
192
60.978
−33.891
4.319
1.00
22.36
A
C


ATOM
1509
CG
LEU
A
192
62.413
−34.264
4.632
1.00
31.34
A
C


ATOM
1510
CD1
LEU
A
192
62.772
−35.494
3.770
1.00
26.77
A
C


ATOM
1511
CD2
LEU
A
192
63.359
−33.091
4.320
1.00
29.88
A
C


ATOM
1512
C
LEU
A
192
59.094
−32.340
4.341
1.00
20.79
A
C


ATOM
1513
O
LEU
A
192
59.148
−31.339
3.689
1.00
19.46
A
O


ATOM
1514
N
HIS
A
193
57.999
−33.086
4.401
1.00
17.50
A
N


ATOM
1515
CA
HIS
A
193
56.821
−32.732
3.624
1.00
17.24
A
C


ATOM
1516
CB
HIS
A
193
55.788
−33.837
3.780
1.00
14.61
A
C


ATOM
1517
CG
HIS
A
193
54.676
−33.751
2.802
1.00
18.22
A
C


ATOM
1518
ND1
HIS
A
193
54.894
−33.665
1.458
1.00
18.97
A
N


ATOM
1519
CE1
HIS
A
193
53.700
−33.623
0.829
1.00
18.36
A
C


ATOM
1520
NE2
HIS
A
193
52.728
−33.669
1.771
1.00
19.62
A
N


ATOM
1521
CD2
HIS
A
193
53.299
−33.743
2.987
1.00
18.95
A
C


ATOM
1522
C
HIS
A
193
56.185
−31.400
3.964
1.00
17.41
A
C


ATOM
1523
O
HIS
A
193
55.452
−30.829
3.174
1.00
19.14
A
O


ATOM
1524
N
ARG
A
194
56.414
−30.930
5.178
1.00
16.56
A
N


ATOM
1525
CA
ARG
A
194
55.803
−29.723
5.675
1.00
17.28
A
C


ATOM
1526
CB
ARG
A
194
55.233
−29.988
7.064
1.00
16.73
A
C


ATOM
1527
CG
ARG
A
194
54.028
−30.930
7.015
1.00
19.35
A
C


ATOM
1528
CD
ARG
A
194
53.331
−31.021
8.393
1.00
18.87
A
C


ATOM
1529
NE
ARG
A
194
52.218
−31.951
8.267
1.00
20.89
A
N


ATOM
1530
CZ
ARG
A
194
51.433
−32.324
9.259
1.00
21.04
A
C


ATOM
1531
NH1
ARG
A
194
51.656
−31.884
10.459
1.00
20.69
A
N


ATOM
1532
NH2
ARG
A
194
50.448
−33.169
9.039
1.00
20.03
A
N


ATOM
1533
C
ARG
A
194
56.764
−28.511
5.759
1.00
19.33
A
C


ATOM
1534
O
ARG
A
194
56.309
−27.377
5.976
1.00
20.35
A
O


ATOM
1535
N
ARG
A
195
58.055
−28.752
5.574
1.00
16.40
A
N


ATOM
1536
CA
ARG
A
195
59.078
−27.720
5.665
1.00
18.30
A
C


ATOM
1537
CB
ARG
A
195
60.421
−28.339
6.048
1.00
19.38
A
C


ATOM
1538
CG
ARG
A
195
61.441
−27.274
6.475
1.00
23.23
A
C


ATOM
1539
CD
ARG
A
195
62.767
−27.849
7.050
1.00
24.25
A
C


ATOM
1540
NE
ARG
A
195
62.606
−28.724
8.219
1.00
20.99
A
N


ATOM
1541
CZ
ARG
A
195
62.751
−28.349
9.495
1.00
26.86
A
C


ATOM
1542
NH1
ARG
A
195
63.093
−27.113
9.823
1.00
26.57
A
N


ATOM
1543
NH2
ARG
A
195
62.590
−29.220
10.467
1.00
27.07
A
N


ATOM
1544
C
ARG
A
195
59.217
−26.991
4.328
1.00
19.93
A
C


ATOM
1545
O
ARG
A
195
59.170
−27.617
3.293
1.00
19.84
A
O


ATOM
1546
N
THR
A
196
59.429
−25.672
4.355
1.00
16.30
A
N


ATOM
1547
CA
THR
A
196
59.627
−24.921
3.106
1.00
19.56
A
C


ATOM
1548
CB
THR
A
196
59.574
−23.413
3.350
1.00
19.32
A
C


ATOM
1549
OG1
THR
A
196
60.572
−23.099
4.291
1.00
20.58
A
O


ATOM
1550
CG2
THR
A
196
58.206
−23.002
3.903
1.00
18.83
A
C


ATOM
1551
C
THR
A
196
60.978
−25.304
2.491
1.00
16.84
A
C


ATOM
1552
O
THR
A
196
61.868
−25.814
3.159
1.00
19.53
A
O


ATOM
1553
N
GLN
A
197
61.086
−25.091
1.198
1.00
18.95
A
N


ATOM
1554
CA
GLN
A
197
62.152
−25.667
0.394
1.00
17.43
A
C


ATOM
1555
CB
GLN
A
197
61.792
−25.510
−1.087
1.00
19.72
A
C


ATOM
1556
CG
GLN
A
197
62.470
−26.467
−2.029
1.00
21.47
A
C


ATOM
1557
CD
GLN
A
197
63.765
−25.922
−2.604
1.00
24.85
A
C


ATOM
1558
OE1
GLN
A
197
64.341
−24.948
−2.093
1.00
22.95
A
O


ATOM
1559
NE2
GLN
A
197
64.246
−26.559
−3.654
1.00
24.44
A
N


ATOM
1560
C
GLN
A
197
63.482
−24.977
0.751
1.00
18.31
A
C


ATOM
1561
O
GLN
A
197
64.481
−25.656
0.934
1.00
16.91
A
O


ATOM
1562
N
ARG
A
198
63.510
−23.641
0.845
1.00
17.97
A
N


ATOM
1563
CA
ARG
A
198
64.771
−22.925
1.163
1.00
17.51
A
C


ATOM
1564
CB
ARG
A
198
64.627
−21.404
1.010
1.00
18.00
A
C


ATOM
1565
CG
ARG
A
198
64.331
−20.959
−0.395
1.00
19.52
A
C


ATOM
1566
CD
ARG
A
198
65.652
−21.070
−1.293
1.00
19.02
A
C


ATOM
1567
NE
ARG
A
198
65.713
−22.398
−1.940
1.00
17.34
A
N


ATOM
1568
CZ
ARG
A
198
66.673
−22.781
−2.784
1.00
20.87
A
C


ATOM
1569
NH1
ARG
A
198
67.691
−21.975
−3.039
1.00
19.28
A
N


ATOM
1570
NH2
ARG
A
198
66.655
−23.989
−3.324
1.00
18.08
A
N


ATOM
1571
C
ARG
A
198
65.277
−23.251
2.552
1.00
18.33
A
C


ATOM
1572
O
ARG
A
198
66.478
−23.429
2.741
1.00
18.01
A
O


ATOM
1573
N
LEU
A
199
64.374
−23.387
3.531
1.00
18.80
A
N


ATOM
1574
CA
LEU
A
199
64.797
−23.716
4.898
1.00
19.16
A
C


ATOM
1575
CB
LEU
A
199
63.654
−23.615
5.918
1.00
26.01
A
C


ATOM
1576
CG
LEU
A
199
63.350
−22.263
6.543
1.00
25.56
A
C


ATOM
1577
CD1
LEU
A
199
62.169
−22.345
7.534
1.00
29.19
A
C


ATOM
1578
CD2
LEU
A
199
64.632
−21.665
7.204
1.00
23.04
A
C


ATOM
1579
C
LEU
A
199
65.357
−25.113
4.947
1.00
18.37
A
C


ATOM
1580
O
LEU
A
199
66.313
−25.361
5.650
1.00
18.68
A
O


ATOM
1581
N
GLU
A
200
64.751
−26.050
4.218
1.00
17.37
A
N


ATOM
1582
CA
GLU
A
200
65.332
−27.377
4.183
1.00
16.57
A
C


ATOM
1583
CB
GLU
A
200
64.423
−28.365
3.480
1.00
17.92
A
C


ATOM
1584
CG
GLU
A
200
64.969
−29.777
3.325
1.00
21.36
A
C


ATOM
1585
CD
GLU
A
200
65.393
−30.426
4.662
1.00
29.23
A
C


ATOM
1586
OE1
GLU
A
200
64.777
−30.107
5.682
1.00
29.79
A
O


ATOM
1587
OE2
GLU
A
200
66.339
−31.263
4.698
1.00
29.20
A
O


ATOM
1588
C
GLU
A
200
66.712
−27.328
3.492
1.00
16.68
A
C


ATOM
1589
O
GLU
A
200
67.635
−28.029
3.914
1.00
19.27
A
O


ATOM
1590
N
ALA
A
201
66.835
−26.553
2.419
1.00
16.26
A
N


ATOM
1591
CA
ALA
A
201
68.099
−26.503
1.657
1.00
18.75
A
C


ATOM
1592
CB
ALA
A
201
67.938
−25.651
0.362
1.00
16.55
A
C


ATOM
1593
C
ALA
A
201
69.238
−25.975
2.506
1.00
20.06
A
C


ATOM
1594
O
ALA
A
201
70.328
−26.580
2.562
1.00
20.06
A
O


ATOM
1595
N
VAL
A
202
68.997
−24.855
3.183
1.00
20.04
A
N


ATOM
1596
CA
VAL
A
202
70.048
−24.237
3.979
1.00
22.09
A
C


ATOM
1597
CB
VAL
A
202
69.667
−22.787
4.489
1.00
18.59
A
C


ATOM
1598
CG1
VAL
A
202
68.692
−22.850
5.643
1.00
20.29
A
C


ATOM
1599
CG2
VAL
A
202
70.962
−21.960
4.888
1.00
24.36
A
C


ATOM
1600
C
VAL
A
202
70.486
−25.181
5.089
1.00
20.38
A
C


ATOM
1601
O
VAL
A
202
71.669
−25.348
5.318
1.00
17.33
A
O


ATOM
1602
N
TRP
A
203
69.556
−25.864
5.729
1.00
20.48
A
N


ATOM
1603
CA
TRP
A
203
69.916
−26.851
6.740
1.00
19.93
A
C


ATOM
1604
CB
TRP
A
203
68.652
−27.360
7.456
1.00
21.30
A
C


ATOM
1605
CG
TRP
A
203
68.975
−28.249
8.620
1.00
19.15
A
C


ATOM
1606
CD1
TRP
A
203
69.089
−27.877
9.965
1.00
23.17
A
C


ATOM
1607
NE1
TRP
A
203
69.420
−28.947
10.731
1.00
26.66
A
N


ATOM
1608
CE2
TRP
A
203
69.529
−30.047
9.981
1.00
23.99
A
C


ATOM
1609
CD2
TRP
A
203
69.253
−29.660
8.596
1.00
19.28
A
C


ATOM
1610
CE3
TRP
A
203
69.265
−30.637
7.605
1.00
20.86
A
C


ATOM
1611
CZ3
TRP
A
203
69.597
−31.925
7.967
1.00
23.16
A
C


ATOM
1612
CH2
TRP
A
203
69.885
−32.271
9.304
1.00
23.58
A
C


ATOM
1613
CZ2
TRP
A
203
69.845
−31.341
10.331
1.00
26.26
A
C


ATOM
1614
C
TRP
A
203
70.677
−28.026
6.162
1.00
24.21
A
C


ATOM
1615
O
TRP
A
203
71.669
−28.502
6.737
1.00
19.37
A
O


ATOM
1616
N
SER
A
204
70.205
−28.545
5.034
1.00
19.86
A
N


ATOM
1617
CA
SER
A
204
70.781
−29.787
4.465
1.00
15.86
A
C


ATOM
1618
CB
SER
A
204
69.853
−30.348
3.371
1.00
17.81
A
C


ATOM
1619
OG
SER
A
204
68.780
−31.055
3.959
1.00
24.34
A
O


ATOM
1620
C
SER
A
204
72.162
−29.573
3.882
1.00
15.58
A
C


ATOM
1621
O
SER
A
204
72.984
−30.467
3.932
1.00
15.50
A
O


ATOM
1622
N
ILE
A
205
72.424
−28.384
3.360
1.00
15.58
A
N


ATOM
1623
CA
ILE
A
205
73.743
−28.077
2.769
1.00
15.73
A
C


ATOM
1624
CB
ILE
A
205
73.725
−26.745
2.001
1.00
14.57
A
C


ATOM
1625
CG1
ILE
A
205
72.923
−26.884
0.700
1.00
14.65
A
C


ATOM
1626
CD1
ILE
A
205
72.469
−25.560
0.102
1.00
16.39
A
C


ATOM
1627
CG2
ILE
A
205
75.213
−26.207
1.740
1.00
16.26
A
C


ATOM
1628
C
ILE
A
205
74.790
−28.051
3.900
1.00
21.28
A
C


ATOM
1629
O
ILE
A
205
75.853
−28.632
3.780
1.00
21.22
A
O


ATOM
1630
N
GLU
A
206
74.450
−27.413
5.011
1.00
23.84
A
N


ATOM
1631
CA
GLU
A
206
75.317
−27.397
6.189
1.00
24.36
A
C


ATOM
1632
CB
GLU
A
206
74.727
−26.476
7.265
1.00
27.57
A
C


ATOM
1633
CG
GLU
A
206
75.503
−26.404
8.572
1.00
34.12
A
C


ATOM
1634
CD
GLU
A
206
76.948
−25.902
8.395
1.00
40.13
A
C


ATOM
1635
OE1
GLU
A
206
77.201
−25.053
7.510
1.00
41.90
A
O


ATOM
1636
OE2
GLU
A
206
77.825
−26.372
9.146
1.00
36.22
A
O


ATOM
1637
C
GLU
A
206
75.548
−28.811
6.739
1.00
26.16
A
C


ATOM
1638
O
GLU
A
206
76.663
−29.138
7.087
1.00
25.64
A
O


ATOM
1639
N
ALA
A
207
74.525
−29.660
6.800
1.00
23.96
A
N


ATOM
1640
CA
ALA
A
207
74.720
−31.038
7.278
1.00
25.61
A
C


ATOM
1641
CB
ALA
A
207
73.383
−31.727
7.520
1.00
25.10
A
C


ATOM
1642
C
ALA
A
207
75.526
−31.852
6.277
1.00
27.54
A
C


ATOM
1643
O
ALA
A
207
76.380
−32.651
6.650
1.00
22.60
A
O


ATOM
1644
N
TYR
A
208
75.245
−31.649
4.992
1.00
21.54
A
N


ATOM
1645
CA
TYR
A
208
75.901
−32.439
3.977
1.00
22.48
A
C


ATOM
1646
CB
TYR
A
208
75.252
−32.220
2.611
1.00
21.04
A
C


ATOM
1647
CG
TYR
A
208
75.584
−33.267
1.584
1.00
20.80
A
C


ATOM
1648
CD1
TYR
A
208
75.197
−34.578
1.753
1.00
23.40
A
C


ATOM
1649
CE1
TYR
A
208
75.505
−35.553
0.790
1.00
24.71
A
C


ATOM
1650
CZ
TYR
A
208
76.171
−35.188
−0.356
1.00
21.51
A
C


ATOM
1651
OH
TYR
A
208
76.466
−36.123
−1.337
1.00
23.71
A
O


ATOM
1652
CE2
TYR
A
208
76.540
−33.885
−0.544
1.00
19.95
A
C


ATOM
1653
CD2
TYR
A
208
76.241
−32.933
0.415
1.00
23.60
A
C


ATOM
1654
C
TYR
A
208
77.399
−32.120
3.924
1.00
18.26
A
C


ATOM
1655
O
TYR
A
208
78.193
−33.017
3.780
1.00
20.93
A
O


ATOM
1656
N
ARG
A
209
77.765
−30.847
4.048
1.00
18.60
A
N


ATOM
1657
CA
ARG
A
209
79.175
−30.438
3.930
1.00
22.43
A
C


ATOM
1658
CB
ARG
A
209
79.303
−28.908
3.801
1.00
25.30
A
C


ATOM
1659
CG
ARG
A
209
79.204
−28.103
5.093
1.00
25.28
A
C


ATOM
1660
CD
ARG
A
209
79.052
−26.620
4.805
1.00
25.54
A
C


ATOM
1661
NE
ARG
A
209
80.121
−26.093
3.948
1.00
20.42
A
N


ATOM
1662
CZ
ARG
A
209
80.063
−24.937
3.292
1.00
19.21
A
C


ATOM
1663
NH1
ARG
A
209
79.010
−24.153
3.373
1.00
17.64
A
N


ATOM
1664
NH2
ARG
A
209
81.080
−24.549
2.541
1.00
22.99
A
N


ATOM
1665
C
ARG
A
209
80.054
−30.985
5.062
1.00
21.25
A
C


ATOM
1666
O
ARG
A
209
81.276
−31.024
4.918
1.00
24.92
A
O


ATOM
1667
N
LYS
A
210
79.429
−31.434
6.153
1.00
25.30
A
N


ATOM
1668
CA
LYS
A
210
80.128
−32.031
7.309
1.00
31.88
A
C


ATOM
1669
CB
LYS
A
210
79.386
−31.701
8.603
1.00
30.97
A
C


ATOM
1670
CG
LYS
A
210
79.381
−30.211
8.900
1.00
34.55
A
C


ATOM
1671
CD
LYS
A
210
78.957
−29.897
10.320
1.00
39.50
A
C


ATOM
1672
CE
LYS
A
210
77.473
−29.640
10.455
1.00
40.90
A
C


ATOM
1673
NZ
LYS
A
210
77.293
−28.453
11.347
1.00
46.57
A
N


ATOM
1674
C
LYS
A
210
80.314
−33.540
7.196
1.00
36.30
A
C


ATOM
1675
O
LYS
A
210
81.035
−34.138
7.998
1.00
33.45
A
O


ATOM
1676
N
LYS
A
211
79.695
−34.155
6.199
1.00
29.38
A
N


ATOM
1677
CA
LYS
A
211
79.869
−35.591
5.976
1.00
34.50
A
C


ATOM
1678
CB
LYS
A
211
78.766
−36.148
5.098
1.00
38.26
A
C


ATOM
1679
CG
LYS
A
211
77.422
−36.240
5.729
1.00
42.79
A
C


ATOM
1680
CD
LYS
A
211
76.481
−36.952
4.765
1.00
46.28
A
C


ATOM
1681
CE
LYS
A
211
75.039
−36.901
5.231
1.00
63.15
A
C


ATOM
1682
NZ
LYS
A
211
74.856
−37.288
6.655
1.00
77.84
A
N


ATOM
1683
C
LYS
A
211
81.171
−35.854
5.257
1.00
43.74
A
C


ATOM
1684
O
LYS
A
211
81.468
−35.222
4.241
1.00
40.90
A
O


ATOM
1685
N
GLU
A
212
81.930
−36.824
5.749
1.00
48.80
A
N


ATOM
1686
CA
GLU
A
212
83.211
−37.148
5.155
1.00
47.55
A
C


ATOM
1687
CB
GLU
A
212
83.947
−38.165
6.026
1.00
57.33
A
C


ATOM
1688
CG
GLU
A
212
85.324
−38.528
5.502
1.00
64.50
A
C


ATOM
1689
CD
GLU
A
212
86.295
−38.925
6.598
1.00
68.42
A
C


ATOM
1690
OE1
GLU
A
212
86.427
−38.173
7.585
1.00
64.80
A
O


ATOM
1691
OE2
GLU
A
212
86.943
−39.983
6.461
1.00
73.66
A
O


ATOM
1692
C
GLU
A
212
83.052
−37.682
3.734
1.00
38.45
A
C


ATOM
1693
O
GLU
A
212
83.865
−37.394
2.868
1.00
40.13
A
O


ATOM
1694
N
ASP
A
213
82.002
−38.447
3.493
1.00
30.53
A
N


ATOM
1695
CA
ASP
A
213
81.793
−39.045
2.173
1.00
34.13
A
C


ATOM
1696
CB
ASP
A
213
81.274
−40.477
2.347
1.00
37.83
A
C


ATOM
1697
CG
ASP
A
213
79.945
−40.536
3.105
1.00
46.70
A
C


ATOM
1698
OD1
ASP
A
213
79.565
−39.534
3.762
1.00
46.50
A
O


ATOM
1699
OD2
ASP
A
213
79.282
−41.591
3.047
1.00
59.21
A
O


ATOM
1700
C
ASP
A
213
80.808
−38.241
1.282
1.00
27.38
A
C


ATOM
1701
O
ASP
A
213
80.218
−38.789
0.379
1.00
27.59
A
O


ATOM
1702
N
ALA
A
214
80.633
−36.954
1.542
1.00
24.53
A
N


ATOM
1703
CA
ALA
A
214
79.766
−36.093
0.706
1.00
26.48
A
C


ATOM
1704
CB
ALA
A
214
79.820
−34.662
1.216
1.00
23.28
A
C


ATOM
1705
C
ALA
A
214
80.212
−36.107
−0.745
1.00
23.11
A
C


ATOM
1706
O
ALA
A
214
81.385
−35.966
−1.014
1.00
17.41
A
O


ATOM
1707
N
ASN
A
215
79.290
−36.226
−1.689
1.00
20.40
A
N


ATOM
1708
CA
ASN
A
215
79.662
−36.045
−3.091
1.00
19.94
A
C


ATOM
1709
CB
ASN
A
215
78.481
−36.438
−3.979
1.00
25.05
A
C


ATOM
1710
CG
ASN
A
215
78.811
−36.376
−5.455
1.00
29.07
A
C


ATOM
1711
OD1
ASN
A
215
79.116
−35.319
−5.995
1.00
23.21
A
O


ATOM
1712
ND2
ASN
A
215
78.759
−37.526
−6.116
1.00
39.39
A
N


ATOM
1713
C
ASN
A
215
80.049
−34.567
−3.313
1.00
16.76
A
C


ATOM
1714
O
ASN
A
215
79.233
−33.686
−3.091
1.00
19.63
A
O


ATOM
1715
N
GLN
A
216
81.279
−34.296
−3.726
1.00
18.59
A
N


ATOM
1716
CA
GLN
A
216
81.769
−32.918
−3.808
1.00
19.77
A
C


ATOM
1717
CB
GLN
A
216
83.296
−32.876
−3.881
1.00
21.22
A
C


ATOM
1718
CG
GLN
A
216
84.004
−33.430
−2.618
1.00
25.97
A
C


ATOM
1719
CD
GLN
A
216
83.625
−32.675
−1.350
1.00
27.20
A
C


ATOM
1720
OE1
GLN
A
216
84.041
−31.531
−1.137
1.00
34.44
A
O


ATOM
1721
NE2
GLN
A
216
82.828
−33.313
−0.506
1.00
31.17
A
N


ATOM
1722
C
GLN
A
216
81.171
−32.104
−4.970
1.00
16.13
A
C


ATOM
1723
O
GLN
A
216
81.006
−30.891
−4.863
1.00
15.04
A
O


ATOM
1724
N
VAL
A
217
80.888
−32.768
−6.076
1.00
16.85
A
N


ATOM
1725
CA
VAL
A
217
80.253
−32.131
−7.235
1.00
16.12
A
C


ATOM
1726
CB
VAL
A
217
80.180
−33.125
−8.471
1.00
16.39
A
C


ATOM
1727
CG1
VAL
A
217
79.233
−32.609
−9.539
1.00
14.97
A
C


ATOM
1728
CG2
VAL
A
217
81.618
−33.384
−9.101
1.00
16.56
A
C


ATOM
1729
C
VAL
A
217
78.852
−31.674
−6.795
1.00
14.34
A
C


ATOM
1730
O
VAL
A
217
78.471
−30.506
−6.964
1.00
15.07
A
O


ATOM
1731
N
LEU
A
218
78.118
−32.552
−6.121
1.00
16.11
A
N


ATOM
1732
CA
LEU
A
218
76.761
−32.202
−5.681
1.00
15.34
A
C


ATOM
1733
CB
LEU
A
218
76.050
−33.463
−5.170
1.00
17.72
A
C


ATOM
1734
CG
LEU
A
218
74.639
−33.315
−4.635
1.00
19.99
A
C


ATOM
1735
CD1
LEU
A
218
73.748
−32.679
−5.719
1.00
17.34
A
C


ATOM
1736
CD2
LEU
A
218
74.146
−34.715
−4.192
1.00
20.61
A
C


ATOM
1737
C
LEU
A
218
76.761
−31.050
−4.633
1.00
16.80
A
C


ATOM
1738
O
LEU
A
218
75.990
−30.073
−4.754
1.00
16.08
A
O


ATOM
1739
N
LEU
A
219
77.667
−31.137
−3.659
1.00
16.22
A
N


ATOM
1740
CA
LEU
A
219
77.808
−30.114
−2.624
1.00
15.98
A
C


ATOM
1741
CB
LEU
A
219
78.878
−30.531
−1.585
1.00
19.49
A
C


ATOM
1742
CG
LEU
A
219
79.274
−29.480
−0.524
1.00
20.60
A
C


ATOM
1743
CD1
LEU
A
219
78.007
−28.966
0.234
1.00
17.64
A
C


ATOM
1744
CD2
LEU
A
219
80.343
−30.018
0.454
1.00
22.47
A
C


ATOM
1745
C
LEU
A
219
78.154
−28.773
−3.243
1.00
14.57
A
C


ATOM
1746
O
LEU
A
219
77.528
−27.773
−2.958
1.00
15.80
A
O


ATOM
1747
N
GLU
A
220
79.152
−28.745
−4.119
1.00
16.34
A
N


ATOM
1748
CA
GLU
A
220
79.521
−27.477
−4.740
1.00
17.54
A
C


ATOM
1749
CB
GLU
A
220
80.762
−27.610
−5.633
1.00
21.32
A
C


ATOM
1750
CG
GLU
A
220
81.228
−26.244
−6.097
1.00
18.06
A
C


ATOM
1751
CD
GLU
A
220
82.556
−26.245
−6.841
1.00
19.47
A
C


ATOM
1752
OE1
GLU
A
220
83.087
−27.308
−7.177
1.00
22.10
A
O


ATOM
1753
OE2
GLU
A
220
83.063
−25.137
−7.085
1.00
22.89
A
O


ATOM
1754
C
GLU
A
220
78.361
−26.863
−5.517
1.00
17.83
A
C


ATOM
1755
O
GLU
A
220
78.059
−25.668
−5.385
1.00
14.53
A
O


ATOM
1756
N
LEU
A
221
77.673
−27.671
−6.298
1.00
17.91
A
N


ATOM
1757
CA
LEU
A
221
76.504
−27.169
−7.040
1.00
14.02
A
C


ATOM
1758
CB
LEU
A
221
75.938
−28.265
−7.935
1.00
14.70
A
C


ATOM
1759
CG
LEU
A
221
74.714
−27.934
−8.808
1.00
13.25
A
C


ATOM
1760
CD1
LEU
A
221
75.094
−26.855
−9.814
1.00
12.26
A
C


ATOM
1761
CD2
LEU
A
221
74.222
−29.193
−9.524
1.00
13.16
A
C


ATOM
1762
C
LEU
A
221
75.421
−26.646
−6.110
1.00
15.19
A
C


ATOM
1763
O
LEU
A
221
74.817
−25.598
−6.358
1.00
13.89
A
O


ATOM
1764
N
ALA
A
222
75.169
−27.359
−5.026
1.00
15.41
A
N


ATOM
1765
CA
ALA
A
222
74.139
−26.940
−4.103
1.00
14.27
A
C


ATOM
1766
CB
ALA
A
222
73.951
−28.006
−2.985
1.00
14.45
A
C


ATOM
1767
C
ALA
A
222
74.440
−25.560
−3.522
1.00
14.90
A
C


ATOM
1768
O
ALA
A
222
73.558
−24.691
−3.439
1.00
16.28
A
O


ATOM
1769
N
ILE
A
223
75.697
−25.329
−3.142
1.00
15.61
A
N


ATOM
1770
CA
ILE
A
223
76.075
−24.058
−2.519
1.00
14.09
A
C


ATOM
1771
CB
ILE
A
223
77.530
−24.124
−2.011
1.00
14.49
A
C


ATOM
1772
CG1
ILE
A
223
77.614
−25.106
−0.823
1.00
13.12
A
C


ATOM
1773
CD1
ILE
A
223
79.093
−25.623
−0.535
1.00
14.91
A
C


ATOM
1774
CG2
ILE
A
223
78.062
−22.702
−1.641
1.00
13.93
A
C


ATOM
1775
C
ILE
A
223
75.926
−22.963
−3.559
1.00
14.88
A
C


ATOM
1776
O
ILE
A
223
75.318
−21.929
−3.333
1.00
14.14
A
O


ATOM
1777
N
LEU
A
224
76.490
−23.216
−4.733
1.00
16.07
A
N


ATOM
1778
CA
LEU
A
224
76.463
−22.240
−5.833
1.00
16.94
A
C


ATOM
1779
CB
LEU
A
224
77.185
−22.875
−6.990
1.00
21.43
A
C


ATOM
1780
CG
LEU
A
224
77.473
−22.190
−8.287
1.00
33.26
A
C


ATOM
1781
CD1
LEU
A
224
78.682
−22.921
−8.853
1.00
29.28
A
C


ATOM
1782
CD2
LEU
A
224
76.226
−22.298
−9.181
1.00
43.08
A
C


ATOM
1783
C
LEU
A
224
75.017
−21.863
−6.210
1.00
14.97
A
C


ATOM
1784
O
LEU
A
224
74.681
−20.676
−6.349
1.00
15.99
A
O


ATOM
1785
N
ASP
A
225
74.167
−22.872
−6.389
1.00
13.69
A
N


ATOM
1786
CA
ASP
A
225
72.779
−22.639
−6.794
1.00
14.56
A
C


ATOM
1787
CB
ASP
A
225
72.044
−23.971
−7.095
1.00
18.03
A
C


ATOM
1788
CG
ASP
A
225
70.792
−23.759
−7.974
1.00
18.76
A
C


ATOM
1789
OD1
ASP
A
225
70.935
−23.627
−9.221
1.00
22.17
A
O


ATOM
1790
OD2
ASP
A
225
69.674
−23.721
−7.410
1.00
18.19
A
O


ATOM
1791
C
ASP
A
225
72.010
−21.858
−5.717
1.00
15.07
A
C


ATOM
1792
O
ASP
A
225
71.264
−20.926
−6.025
1.00
13.40
A
O


ATOM
1793
N
TYR
A
226
72.198
−22.244
−4.462
1.00
14.97
A
N


ATOM
1794
CA
TYR
A
226
71.539
−21.565
−3.347
1.00
15.99
A
C


ATOM
1795
CB
TYR
A
226
71.895
−22.238
−2.014
1.00
18.96
A
C


ATOM
1796
CG
TYR
A
226
71.124
−21.654
−0.849
1.00
20.01
A
C


ATOM
1797
CD1
TYR
A
226
71.531
−20.468
−0.232
1.00
22.17
A
C


ATOM
1798
CE1
TYR
A
226
70.811
−19.919
0.830
1.00
19.62
A
C


ATOM
1799
CZ
TYR
A
226
69.685
−20.564
1.285
1.00
21.40
A
C


ATOM
1800
OH
TYR
A
226
68.962
−20.057
2.317
1.00
22.99
A
O


ATOM
1801
CE2
TYR
A
226
69.274
−21.758
0.711
1.00
17.96
A
C


ATOM
1802
CD2
TYR
A
226
69.990
−22.288
−0.364
1.00
17.80
A
C


ATOM
1803
C
TYR
A
226
71.934
−20.074
−3.287
1.00
16.06
A
C


ATOM
1804
O
TYR
A
226
71.069
−19.202
−3.090
1.00
16.88
A
O


ATOM
1805
N
ASN
A
227
73.218
−19.782
−3.461
1.00
14.97
A
N


ATOM
1806
CA
ASN
A
227
73.689
−18.390
−3.418
1.00
18.29
A
C


ATOM
1807
CB
ASN
A
227
75.208
−18.332
−3.217
1.00
17.52
A
C


ATOM
1808
CG
ASN
A
227
75.601
−18.718
−1.803
1.00
19.83
A
C


ATOM
1809
OD1
ASN
A
227
74.751
−18.764
−0.907
1.00
19.76
A
O


ATOM
1810
ND2
ASN
A
227
76.878
−18.990
−1.584
1.00
17.61
A
N


ATOM
1811
C
ASN
A
227
73.218
−17.584
−4.611
1.00
19.68
A
C


ATOM
1812
O
ASN
A
227
72.905
−16.409
−4.480
1.00
20.30
A
O


ATOM
1813
N
MET
A
228
73.103
−18.230
−5.763
1.00
17.59
A
N


ATOM
1814
CA
MET
A
228
72.499
−17.585
−6.919
1.00
19.81
A
C


ATOM
1815
CB
MET
A
228
72.586
−18.489
−8.115
1.00
21.31
A
C


ATOM
1816
CG
MET
A
228
71.922
−17.937
−9.338
1.00
24.26
A
C


ATOM
1817
SD
MET
A
228
71.839
−19.226
−10.569
1.00
31.75
A
S


ATOM
1818
CE
MET
A
228
70.334
−20.104
−10.032
1.00
32.05
A
C


ATOM
1819
C
MET
A
228
71.029
−17.237
−6.679
1.00
21.09
A
C


ATOM
1820
O
MET
A
228
70.575
−16.114
−6.923
1.00
22.56
A
O


ATOM
1821
N
ILE
A
229
70.248
−18.207
−6.248
1.00
20.81
A
N


ATOM
1822
CA
ILE
A
229
68.850
−17.912
−5.976
1.00
18.17
A
C


ATOM
1823
CB
ILE
A
229
68.039
−19.161
−5.543
1.00
19.71
A
C


ATOM
1824
CG1
ILE
A
229
67.902
−20.131
−6.713
1.00
17.07
A
C


ATOM
1825
CD1
ILE
A
229
67.115
−21.438
−6.387
1.00
18.64
A
C


ATOM
1826
CG2
ILE
A
229
66.597
−18.738
−5.059
1.00
16.81
A
C


ATOM
1827
C
ILE
A
229
68.766
−16.773
−4.941
1.00
18.94
A
C


ATOM
1828
O
ILE
A
229
67.982
−15.852
−5.126
1.00
20.45
A
O


ATOM
1829
N
GLN
A
230
69.565
−16.828
−3.874
1.00
19.83
A
N


ATOM
1830
CA
GLN
A
230
69.529
−15.780
−2.851
1.00
26.24
A
C


ATOM
1831
CB
GLN
A
230
70.548
−16.041
−1.742
1.00
23.79
A
C


ATOM
1832
CG
GLN
A
230
70.402
−15.118
−0.572
1.00
24.95
A
C


ATOM
1833
CD
GLN
A
230
71.409
−15.419
0.507
1.00
25.13
A
C


ATOM
1834
OE1
GLN
A
230
72.601
−15.204
0.324
1.00
27.12
A
O


ATOM
1835
NE2
GLN
A
230
70.944
−15.958
1.626
1.00
21.51
A
N


ATOM
1836
C
GLN
A
230
69.785
−14.383
−3.452
1.00
25.05
A
C


ATOM
1837
O
GLN
A
230
69.148
−13.425
−3.038
1.00
24.32
A
O


ATOM
1838
N
SER
A
231
70.692
−14.271
−4.425
1.00
20.12
A
N


ATOM
1839
CA
SER
A
231
70.944
−12.970
−5.061
1.00
23.39
A
C


ATOM
1840
CB
SER
A
231
72.235
−12.977
−5.917
1.00
24.86
A
C


ATOM
1841
OG
SER
A
231
72.010
−13.749
−7.098
1.00
38.15
A
O


ATOM
1842
C
SER
A
231
69.741
−12.524
−5.896
1.00
22.11
A
C


ATOM
1843
O
SER
A
231
69.523
−11.336
−6.058
1.00
19.49
A
O


ATOM
1844
N
VAL
A
232
68.953
−13.453
−6.425
1.00
20.92
A
N


ATOM
1845
CA
VAL
A
232
67.641
−13.085
−7.019
1.00
22.15
A
C


ATOM
1846
CB
VAL
A
232
67.005
−14.238
−7.790
1.00
21.81
A
C


ATOM
1847
CG1
VAL
A
232
65.573
−13.889
−8.300
1.00
21.93
A
C


ATOM
1848
CG2
VAL
A
232
67.928
−14.622
−8.989
1.00
20.61
A
C


ATOM
1849
C
VAL
A
232
66.712
−12.494
−5.946
1.00
24.91
A
C


ATOM
1850
O
VAL
A
232
66.149
−11.407
−6.113
1.00
23.40
A
O


ATOM
1851
N
TYR
A
233
66.617
−13.149
−4.808
1.00
24.97
A
N


ATOM
1852
CA
TYR
A
233
65.789
−12.616
−3.724
1.00
23.17
A
C


ATOM
1853
CB
TYR
A
233
65.824
−13.529
−2.505
1.00
20.84
A
C


ATOM
1854
CG
TYR
A
233
65.292
−14.943
−2.719
1.00
17.60
A
C


ATOM
1855
CD1
TYR
A
233
64.476
−15.277
−3.807
1.00
16.71
A
C


ATOM
1856
CE1
TYR
A
233
64.019
−16.589
−3.967
1.00
18.85
A
C


ATOM
1857
CZ
TYR
A
233
64.370
−17.529
−3.020
1.00
16.53
A
C


ATOM
1858
OH
TYR
A
233
63.953
−18.838
−3.124
1.00
19.31
A
O


ATOM
1859
CE2
TYR
A
233
65.151
−17.207
−1.969
1.00
17.10
A
C


ATOM
1860
CD2
TYR
A
233
65.582
−15.937
−1.792
1.00
17.40
A
C


ATOM
1861
C
TYR
A
233
66.262
−11.215
−3.280
1.00
26.79
A
C


ATOM
1862
O
TYR
A
233
65.442
−10.334
−3.009
1.00
25.17
A
O


ATOM
1863
N
GLN
A
234
67.575
−11.021
−3.153
1.00
26.50
A
N


ATOM
1864
CA
GLN
A
234
68.093
−9.703
−2.738
1.00
26.52
A
C


ATOM
1865
CB
GLN
A
234
69.603
−9.768
−2.474
1.00
25.75
A
C


ATOM
1866
CG
GLN
A
234
69.927
−10.604
−1.214
1.00
27.64
A
C


ATOM
1867
CD
GLN
A
234
71.356
−11.093
−1.178
1.00
30.47
A
C


ATOM
1868
OE1
GLN
A
234
72.087
−10.905
−2.136
1.00
34.88
A
O


ATOM
1869
NE2
GLN
A
234
71.761
−11.729
−0.077
1.00
30.17
A
N


ATOM
1870
C
GLN
A
234
67.689
−8.590
−3.739
1.00
29.34
A
C


ATOM
1871
O
GLN
A
234
67.342
−7.459
−3.326
1.00
30.77
A
O


ATOM
1872
N
ARG
A
235
67.721
−8.905
−5.031
1.00
24.11
A
N


ATOM
1873
CA
ARG
A
235
67.249
−7.974
−6.056
1.00
29.32
A
C


ATOM
1874
CB
ARG
A
235
67.585
−8.460
−7.467
1.00
30.12
A
C


ATOM
1875
CG
ARG
A
235
67.211
−7.417
−8.543
1.00
42.25
A
C


ATOM
1876
CD
ARG
A
235
67.848
−7.655
−9.904
1.00
50.89
A
C


ATOM
1877
NE
ARG
A
235
67.654
−9.034
−10.339
1.00
80.51
A
N


ATOM
1878
CZ
ARG
A
235
68.596
−9.984
−10.361
1.00
95.81
A
C


ATOM
1879
NH1
ARG
A
235
69.853
−9.730
−9.995
1.00
85.77
A
N


ATOM
1880
NH2
ARG
A
235
68.275
−11.211
−10.768
1.00
87.26
A
N


ATOM
1881
C
ARG
A
235
65.740
−7.693
−5.936
1.00
31.87
A
C


ATOM
1882
O
ARG
A
235
65.299
−6.546
−5.996
1.00
26.48
A
O


ATOM
1883
N
ASP
A
236
64.950
−8.739
−5.774
1.00
26.15
A
N


ATOM
1884
CA
ASP
A
236
63.512
−8.589
−5.551
1.00
25.59
A
C


ATOM
1885
CB
ASP
A
236
62.856
−9.930
−5.212
1.00
27.19
A
C


ATOM
1886
CG
ASP
A
236
62.892
−10.974
−6.355
1.00
28.21
A
C


ATOM
1887
OD1
ASP
A
236
63.016
−10.622
−7.529
1.00
32.32
A
O


ATOM
1888
OD2
ASP
A
236
62.734
−12.184
−6.030
1.00
30.52
A
O


ATOM
1889
C
ASP
A
236
63.217
−7.666
−4.368
1.00
22.39
A
C


ATOM
1890
O
ASP
A
236
62.342
−6.826
−4.438
1.00
25.74
A
O


ATOM
1891
N
LEU
A
237
63.910
−7.896
−3.263
1.00
18.81
A
N


ATOM
1892
CA
LEU
A
237
63.675
−7.218
−2.040
1.00
21.93
A
C


ATOM
1893
CB
LEU
A
237
64.401
−7.923
−0.907
1.00
25.78
A
C


ATOM
1894
CG
LEU
A
237
64.373
−7.206
0.428
1.00
32.12
A
C


ATOM
1895
CD1
LEU
A
237
62.938
−7.158
0.902
1.00
33.09
A
C


ATOM
1896
CD2
LEU
A
237
65.279
−7.873
1.452
1.00
33.88
A
C


ATOM
1897
C
LEU
A
237
64.130
−5.761
−2.147
1.00
29.65
A
C


ATOM
1898
O
LEU
A
237
63.507
−4.869
−1.577
1.00
30.17
A
O


ATOM
1899
N
ARG
A
238
65.196
−5.506
−2.898
1.00
25.49
A
N


ATOM
1900
CA
ARG
A
238
65.656
−4.127
−3.044
1.00
34.24
A
C


ATOM
1901
CB
ARG
A
238
67.022
−4.032
−3.752
1.00
31.76
A
C


ATOM
1902
CG
ARG
A
238
68.209
−3.896
−2.789
1.00
37.91
A
C


ATOM
1903
CD
ARG
A
238
69.513
−3.536
−3.537
1.00
38.29
A
C


ATOM
1904
NE
ARG
A
238
69.709
−4.359
−4.738
1.00
35.82
A
N


ATOM
1905
CZ
ARG
A
238
70.261
−5.583
−4.763
1.00
41.70
A
C


ATOM
1906
NH1
ARG
A
238
70.718
−6.162
−3.649
1.00
35.80
A
N


ATOM
1907
NH2
ARG
A
238
70.363
−6.243
−5.923
1.00
32.91
A
N


ATOM
1908
C
ARG
A
238
64.583
−3.336
−3.786
1.00
34.60
A
C


ATOM
1909
O
ARG
A
238
64.244
−2.223
−3.375
1.00
28.36
A
O


ATOM
1910
N
GLU
A
239
64.026
−3.931
−4.840
1.00
30.53
A
N


ATOM
1911
CA
GLU
A
239
62.927
−3.310
−5.587
1.00
38.02
A
C


ATOM
1912
CB
GLU
A
239
62.596
−4.159
−6.798
1.00
45.08
A
C


ATOM
1913
CG
GLU
A
239
61.458
−3.618
−7.638
1.00
66.28
A
C


ATOM
1914
CD
GLU
A
239
61.478
−4.172
−9.051
1.00
88.80
A
C


ATOM
1915
OE1
GLU
A
239
61.348
−5.403
−9.209
1.00
95.01
A
O


ATOM
1916
OE2
GLU
A
239
61.631
−3.376
−10.002
1.00
102.21
A
O


ATOM
1917
C
GLU
A
239
61.651
−3.123
−4.740
1.00
37.69
A
C


ATOM
1918
O
GLU
A
239
60.985
−2.089
−4.779
1.00
41.09
A
O


ATOM
1919
N
THR
A
240
61.308
−4.132
−3.973
1.00
29.30
A
N


ATOM
1920
CA
THR
A
240
60.119
−4.032
−3.133
1.00
30.80
A
C


ATOM
1921
CB
THR
A
240
59.735
−5.409
−2.610
1.00
35.45
A
C


ATOM
1922
OG1
THR
A
240
59.434
−6.224
−3.753
1.00
34.87
A
O


ATOM
1923
CG2
THR
A
240
58.528
−5.342
−1.677
1.00
31.60
A
C


ATOM
1924
C
THR
A
240
60.340
−3.000
−2.021
1.00
29.08
A
C


ATOM
1925
O
THR
A
240
59.404
−2.285
−1.637
1.00
25.40
A
O


ATOM
1926
N
SER
A
241
61.574
−2.927
−1.523
1.00
31.82
A
N


ATOM
1927
CA
SER
A
241
61.967
−1.904
−0.546
1.00
35.69
A
C


ATOM
1928
CB
SER
A
241
63.390
−2.135
−0.049
1.00
38.03
A
C


ATOM
1929
OG
SER
A
241
63.376
−3.275
0.796
1.00
38.86
A
O


ATOM
1930
C
SER
A
241
61.811
−0.492
−1.092
1.00
32.52
A
C


ATOM
1931
O
SER
A
241
61.262
0.355
−0.407
1.00
32.27
A
O


ATOM
1932
N
ARG
A
242
62.267
−0.251
−2.316
1.00
38.08
A
N


ATOM
1933
CA
ARG
A
242
62.095
1.061
−2.939
1.00
45.47
A
C


ATOM
1934
CB
ARG
A
242
62.647
1.107
−4.362
1.00
55.62
A
C


ATOM
1935
CG
ARG
A
242
64.105
1.575
−4.465
1.00
60.65
A
C


ATOM
1936
CD
ARG
A
242
64.540
1.737
−5.922
1.00
58.46
A
C


ATOM
1937
NE
ARG
A
242
64.176
0.562
−6.715
1.00
63.57
A
N


ATOM
1938
CZ
ARG
A
242
64.562
0.327
−7.965
1.00
71.15
A
C


ATOM
1939
NH1
ARG
A
242
65.362
1.172
−8.605
1.00
77.17
A
N


ATOM
1940
NH2
ARG
A
242
64.157
−0.786
−8.573
1.00
69.07
A
N


ATOM
1941
C
ARG
A
242
60.633
1.407
−2.984
1.00
36.39
A
C


ATOM
1942
O
ARG
A
242
60.243
2.473
−2.544
1.00
28.12
A
O


ATOM
1943
N
TRP
A
243
59.829
0.492
−3.503
1.00
28.35
A
N


ATOM
1944
CA
TRP
A
243
58.361
0.657
−3.551
1.00
26.03
A
C


ATOM
1945
CB
TRP
A
243
57.695
−0.617
−4.077
1.00
29.56
A
C


ATOM
1946
CG
TRP
A
243
56.200
−0.649
−3.818
1.00
23.64
A
C


ATOM
1947
CD1
TRP
A
243
55.221
0.012
−4.539
1.00
22.96
A
C


ATOM
1948
NE1
TRP
A
243
53.992
−0.215
−4.013
1.00
23.68
A
N


ATOM
1949
CE2
TRP
A
243
54.067
−1.021
−2.931
1.00
19.35
A
C


ATOM
1950
CD2
TRP
A
243
55.475
−1.341
−2.736
1.00
21.40
A
C


ATOM
1951
CE3
TRP
A
243
55.839
−2.117
−1.658
1.00
21.11
A
C


ATOM
1952
CZ3
TRP
A
243
54.839
−2.611
−0.816
1.00
21.65
A
C


ATOM
1953
CH2
TRP
A
243
53.499
−2.292
−1.025
1.00
24.12
A
C


ATOM
1954
CZ2
TRP
A
243
53.098
−1.470
−2.085
1.00
20.86
A
C


ATOM
1955
C
TRP
A
243
57.748
1.020
−2.212
1.00
28.47
A
C


ATOM
1956
O
TRP
A
243
56.898
1.931
−2.117
1.00
26.65
A
O


ATOM
1957
N
TRP
A
244
58.131
0.285
−1.170
1.00
21.36
A
N


ATOM
1958
CA
TRP
A
244
57.522
0.468
0.135
1.00
22.92
A
C


ATOM
1959
CB
TRP
A
244
57.903
−0.689
1.044
1.00
19.70
A
C


ATOM
1960
CG
TRP
A
244
57.271
−0.719
2.405
1.00
23.19
A
C


ATOM
1961
CD1
TRP
A
244
57.923
−0.793
3.634
1.00
32.70
A
C


ATOM
1962
NE1
TRP
A
244
57.011
−0.851
4.665
1.00
32.87
A
N


ATOM
1963
CE2
TRP
A
244
55.764
−0.834
4.204
1.00
24.73
A
C


ATOM
1964
CD2
TRP
A
244
55.837
−0.768
2.746
1.00
23.81
A
C


ATOM
1965
CE3
TRP
A
244
54.669
−0.697
2.027
1.00
25.86
A
C


ATOM
1966
CZ3
TRP
A
244
53.466
−0.723
2.708
1.00
30.63
A
C


ATOM
1967
CH2
TRP
A
244
53.418
−0.815
4.093
1.00
29.59
A
C


ATOM
1968
CZ2
TRP
A
244
54.568
−0.864
4.867
1.00
30.60
A
C


ATOM
1969
C
TRP
A
244
57.894
1.796
0.747
1.00
21.08
A
C


ATOM
1970
O
TRP
A
244
57.066
2.428
1.393
1.00
20.60
A
O


ATOM
1971
N
ARG
A
245
59.131
2.228
0.536
1.00
27.20
A
N


ATOM
1972
CA
ARG
A
245
59.600
3.537
1.011
1.00
31.73
A
C


ATOM
1973
CB
ARG
A
245
61.114
3.701
0.828
1.00
32.00
A
C


ATOM
1974
CG
ARG
A
245
61.957
3.111
1.957
1.00
47.72
A
C


ATOM
1975
CD
ARG
A
245
63.414
3.658
1.909
1.00
65.57
A
C


ATOM
1976
NE
ARG
A
245
63.978
3.622
0.550
1.00
70.19
A
N


ATOM
1977
CZ
ARG
A
245
64.550
2.557
−0.028
1.00
68.85
A
C


ATOM
1978
NH1
ARG
A
245
64.675
1.394
0.614
1.00
60.07
A
N


ATOM
1979
NH2
ARG
A
245
65.007
2.655
−1.273
1.00
76.75
A
N


ATOM
1980
C
ARG
A
245
58.884
4.688
0.316
1.00
32.25
A
C


ATOM
1981
O
ARG
A
245
58.541
5.682
0.955
1.00
25.27
A
O


ATOM
1982
N
ARG
A
246
58.657
4.557
−0.981
1.00
30.10
A
N


ATOM
1983
CA
ARG
A
246
57.876
5.545
−1.716
1.00
35.79
A
C


ATOM
1984
CB
ARG
A
246
57.869
5.189
−3.197
1.00
46.51
A
C


ATOM
1985
CG
ARG
A
246
56.789
5.862
−4.038
1.00
57.62
A
C


ATOM
1986
CD
ARG
A
246
57.095
5.711
−5.519
1.00
77.36
A
C


ATOM
1987
NE
ARG
A
246
58.470
6.129
−5.827
1.00
99.02
A
N


ATOM
1988
CZ
ARG
A
246
59.493
5.319
−6.132
1.00
100.61
A
C


ATOM
1989
NH1
ARG
A
246
59.344
3.996
−6.197
1.00
99.90
A
N


ATOM
1990
NH2
ARG
A
246
60.690
5.849
−6.382
1.00
97.80
A
N


ATOM
1991
C
ARG
A
246
56.451
5.614
−1.172
1.00
28.96
A
C


ATOM
1992
O
ARG
A
246
55.917
6.676
−0.969
1.00
27.23
A
O


ATOM
1993
N
VAL
A
247
55.842
4.472
−0.908
1.00
21.35
A
N


ATOM
1994
CA
VAL
A
247
54.492
4.449
−0.332
1.00
21.70
A
C


ATOM
1995
CB
VAL
A
247
53.980
3.019
−0.174
1.00
21.06
A
C


ATOM
1996
CG1
VAL
A
247
52.639
2.991
0.545
1.00
24.68
A
C


ATOM
1997
CG2
VAL
A
247
53.840
2.363
−1.563
1.00
29.20
A
C


ATOM
1998
C
VAL
A
247
54.523
5.162
1.024
1.00
21.79
A
C


ATOM
1999
O
VAL
A
247
53.668
6.032
1.306
1.00
17.47
A
O


ATOM
2000
N
GLY
A
248
55.514
4.807
1.853
1.00
21.84
A
N


ATOM
2001
CA
GLY
A
248
55.852
5.583
3.069
1.00
24.01
A
C


ATOM
2002
C
GLY
A
248
54.844
5.545
4.220
1.00
25.34
A
C


ATOM
2003
O
GLY
A
248
54.778
6.451
5.052
1.00
31.72
A
O


ATOM
2004
N
LEU
A
249
54.056
4.504
4.269
1.00
26.67
A
N


ATOM
2005
CA
LEU
A
249
52.901
4.445
5.142
1.00
31.07
A
C


ATOM
2006
CB
LEU
A
249
51.968
3.379
4.590
1.00
32.92
A
C


ATOM
2007
CG
LEU
A
249
50.445
3.425
4.652
1.00
34.04
A
C


ATOM
2008
CD1
LEU
A
249
49.811
4.784
4.331
1.00
27.52
A
C


ATOM
2009
CD2
LEU
A
249
49.936
2.336
3.719
1.00
33.60
A
C


ATOM
2010
C
LEU
A
249
53.327
4.192
6.615
1.00
46.12
A
C


ATOM
2011
O
LEU
A
249
52.834
4.864
7.544
1.00
39.23
A
O


ATOM
2012
N
ALA
A
250
54.280
3.276
6.815
1.00
51.58
A
N


ATOM
2013
CA
ALA
A
250
54.779
2.935
8.157
1.00
62.76
A
C


ATOM
2014
CB
ALA
A
250
55.754
1.749
8.093
1.00
57.13
A
C


ATOM
2015
C
ALA
A
250
55.430
4.152
8.828
1.00
61.60
A
C


ATOM
2016
O
ALA
A
250
55.355
4.301
10.041
1.00
69.56
A
O


ATOM
2017
N
THR
A
251
56.045
5.017
8.021
1.00
47.72
A
N


ATOM
2018
CA
THR
A
251
56.545
6.327
8.461
1.00
43.94
A
C


ATOM
2019
CB
THR
A
251
57.291
7.050
7.298
1.00
38.98
A
C


ATOM
2020
OG1
THR
A
251
58.145
6.124
6.625
1.00
60.41
A
O


ATOM
2021
CG2
THR
A
251
58.107
8.197
7.788
1.00
38.02
A
C


ATOM
2022
C
THR
A
251
55.432
7.280
8.926
1.00
44.65
A
C


ATOM
2023
O
THR
A
251
55.573
7.945
9.942
1.00
49.78
A
O


ATOM
2024
N
LYS
A
252
54.342
7.358
8.159
1.00
33.75
A
N


ATOM
2025
CA
LYS
A
252
53.312
8.411
8.329
1.00
29.58
A
C


ATOM
2026
CB
LYS
A
252
52.721
8.780
6.938
1.00
38.55
A
C


ATOM
2027
CG
LYS
A
252
53.751
9.375
5.910
1.00
44.61
A
C


ATOM
2028
CD
LYS
A
252
54.042
10.870
6.119
1.00
38.46
A
C


ATOM
2029
CE
LYS
A
252
55.237
11.331
5.273
1.00
43.27
A
C


ATOM
2030
NZ
LYS
A
252
55.325
12.808
5.140
1.00
40.15
A
N


ATOM
2031
C
LYS
A
252
52.174
8.036
9.302
1.00
25.43
A
C


ATOM
2032
O
LYS
A
252
51.554
8.892
9.885
1.00
36.35
A
O


ATOM
2033
N
LEU
A
253
51.894
6.743
9.435
1.00
28.29
A
N


ATOM
2034
CA
LEU
A
253
50.964
6.233
10.425
1.00
28.58
A
C


ATOM
2035
CB
LEU
A
253
50.221
5.011
9.864
1.00
31.96
A
C


ATOM
2036
CG
LEU
A
253
48.977
5.172
8.971
1.00
37.47
A
C


ATOM
2037
CD1
LEU
A
253
49.006
6.440
8.122
1.00
31.09
A
C


ATOM
2038
CD2
LEU
A
253
48.798
3.903
8.130
1.00
35.88
A
C


ATOM
2039
C
LEU
A
253
51.809
5.811
11.646
1.00
31.04
A
C


ATOM
2040
O
LEU
A
253
52.552
4.821
11.581
1.00
33.71
A
O


ATOM
2041
N
HIS
A
254
51.636
6.515
12.758
1.00
27.06
A
N


ATOM
2042
CA
AHIS
A
254
52.536
6.332
13.904
0.50
29.33
A
C


ATOM
2043
CA
BHIS
A
254
52.460
6.372
13.983
0.50
31.17
A
C


ATOM
2044
CB
AHIS
A
254
52.490
7.564
14.820
0.50
28.81
A
C


ATOM
2045
CB
BHIS
A
254
52.159
7.517
14.992
0.50
32.28
A
C


ATOM
2046
CG
AHIS
A
254
52.561
8.884
14.067
0.50
32.89
A
C


ATOM
2047
CG
BHIS
A
254
50.831
7.378
15.741
0.50
39.44
A
C


ATOM
2048
ND1
AHIS
A
254
53.193
9.013
12.872
0.50
30.44
A
N


ATOM
2049
ND1
BHIS
A
254
49.750
8.139
15.460
0.50
44.49
A
N


ATOM
2050
CE1
AHIS
A
254
53.081
10.287
12.450
0.50
30.02
A
C


ATOM
2051
CE1
BHIS
A
254
48.736
7.798
16.285
0.50
39.97
A
C


ATOM
2052
NE2
AHIS
A
254
52.387
10.977
13.377
0.50
30.22
A
N


ATOM
2053
NE2
BHIS
A
254
49.170
6.817
17.100
0.50
33.73
A
N


ATOM
2054
CD2
AHIS
A
254
52.052
10.145
14.382
0.50
29.37
A
C


ATOM
2055
CD2
BHIS
A
254
50.455
6.542
16.802
0.50
37.01
A
C


ATOM
2056
C
HIS
A
254
52.296
5.040
14.669
1.00
29.00
A
C


ATOM
2057
O
HIS
A
254
53.161
4.596
15.424
1.00
26.96
A
O


ATOM
2058
N
PHE
A
255
51.156
4.403
14.447
1.00
23.47
A
N


ATOM
2059
CA
PHE
A
255
50.822
3.146
15.121
1.00
27.33
A
C


ATOM
2060
CB
PHE
A
255
49.305
3.062
15.358
1.00
25.32
A
C


ATOM
2061
CG
PHE
A
255
48.517
2.984
14.077
1.00
24.28
A
C


ATOM
2062
CD1
PHE
A
255
48.347
1.777
13.432
1.00
26.59
A
C


ATOM
2063
CE1
PHE
A
255
47.663
1.707
12.211
1.00
29.93
A
C


ATOM
2064
CZ
PHE
A
255
47.171
2.861
11.637
1.00
26.82
A
C


ATOM
2065
CE2
PHE
A
255
47.332
4.065
12.270
1.00
34.44
A
C


ATOM
2066
CD2
PHE
A
255
48.027
4.133
13.480
1.00
30.12
A
C


ATOM
2067
C
PHE
A
255
51.275
1.915
14.318
1.00
30.30
A
C


ATOM
2068
O
PHE
A
255
51.227
0.809
14.832
1.00
29.18
A
O


ATOM
2069
N
ALA
A
256
51.680
2.091
13.063
1.00
32.80
A
N


ATOM
2070
CA
ALA
A
256
51.890
0.933
12.166
1.00
39.26
A
C


ATOM
2071
CB
ALA
A
256
51.835
1.360
10.708
1.00
38.98
A
C


ATOM
2072
C
ALA
A
256
53.201
0.205
12.438
1.00
32.90
A
C


ATOM
2073
O
ALA
A
256
54.219
0.833
12.705
1.00
35.46
A
O


ATOM
2074
N
ARG
A
257
53.128
−1.125
12.418
1.00
39.28
A
N


ATOM
2075
CA
ARG
A
257
54.285
−2.021
12.366
1.00
43.61
A
C


ATOM
2076
CB
ARG
A
257
53.852
−3.479
12.677
1.00
45.99
A
C


ATOM
2077
CG
ARG
A
257
53.326
−3.819
14.113
1.00
50.74
A
C


ATOM
2078
CD
ARG
A
257
52.807
−5.303
14.183
1.00
48.09
A
C


ATOM
2079
NE
ARG
A
257
51.532
−5.483
13.477
1.00
37.96
A
N


ATOM
2080
CZ
ARG
A
257
51.080
−6.614
12.921
1.00
48.88
A
C


ATOM
2081
NH1
ARG
A
257
51.799
−7.737
12.951
1.00
53.81
A
N


ATOM
2082
NH2
ARG
A
257
49.889
−6.623
12.284
1.00
39.93
A
N


ATOM
2083
C
ARG
A
257
54.875
−2.001
10.931
1.00
46.39
A
C


ATOM
2084
O
ARG
A
257
54.136
−2.233
9.960
1.00
44.76
A
O


ATOM
2085
N
ASP
A
258
56.182
−1.751
10.804
1.00
44.98
A
N


ATOM
2086
CA
ASP
A
258
56.916
−2.010
9.540
1.00
51.71
A
C


ATOM
2087
CB
ASP
A
258
58.123
−1.065
9.388
1.00
55.16
A
C


ATOM
2088
CG
ASP
A
258
58.906
−1.298
8.082
1.00
63.44
A
C


ATOM
2089
OD1
ASP
A
258
58.551
−2.204
7.297
1.00
55.55
A
O


ATOM
2090
OD2
ASP
A
258
59.895
−0.576
7.838
1.00
65.16
A
O


ATOM
2091
C
ASP
A
258
57.383
−3.491
9.481
1.00
46.48
A
C


ATOM
2092
O
ASP
A
258
58.236
−3.923
10.268
1.00
47.25
A
O


ATOM
2093
N
ARG
A
259
56.824
−4.265
8.553
1.00
39.72
A
N


ATOM
2094
CA
ARG
A
259
57.104
−5.695
8.508
1.00
36.16
A
C


ATOM
2095
CB
ARG
A
259
55.842
−6.489
8.901
1.00
45.84
A
C


ATOM
2096
CG
ARG
A
259
55.467
−6.478
10.387
1.00
48.37
A
C


ATOM
2097
CD
ARG
A
259
56.185
−7.566
11.182
1.00
58.13
A
C


ATOM
2098
NE
ARG
A
259
55.862
−7.511
12.615
1.00
64.71
A
N


ATOM
2099
CZ
ARG
A
259
56.333
−6.609
13.492
1.00
62.43
A
C


ATOM
2100
NH1
ARG
A
259
57.172
−5.644
13.119
1.00
60.33
A
N


ATOM
2101
NH2
ARG
A
259
55.952
−6.663
14.765
1.00
51.48
A
N


ATOM
2102
C
ARG
A
259
57.591
−6.115
7.122
1.00
36.67
A
C


ATOM
2103
O
ARG
A
259
57.303
−7.244
6.673
1.00
27.57
A
O


ATOM
2104
N
LEU
A
260
58.341
−5.256
6.436
1.00
31.46
A
N


ATOM
2105
CA
LEU
A
260
58.687
−5.577
5.030
1.00
31.03
A
C


ATOM
2106
CB
LEU
A
260
59.352
−4.410
4.273
1.00
37.61
A
C


ATOM
2107
CG
LEU
A
260
59.622
−4.758
2.789
1.00
35.78
A
C


ATOM
2108
CD1
LEU
A
260
58.346
−4.883
2.016
1.00
43.44
A
C


ATOM
2109
CD2
LEU
A
260
60.514
−3.785
2.123
1.00
49.39
A
C


ATOM
2110
C
LEU
A
260
59.554
−6.825
4.887
1.00
26.61
A
C


ATOM
2111
O
LEU
A
260
59.309
−7.654
3.992
1.00
25.45
A
O


ATOM
2112
N
ILE
A
261
60.579
−6.957
5.728
1.00
25.93
A
N


ATOM
2113
CA
ILE
A
261
61.526
−8.051
5.533
1.00
28.16
A
C


ATOM
2114
CB
ILE
A
261
62.790
−7.905
6.400
1.00
33.56
A
C


ATOM
2115
CG1
ILE
A
261
63.502
−6.572
6.083
1.00
40.92
A
C


ATOM
2116
CD1
ILE
A
261
64.784
−6.261
6.922
1.00
42.44
A
C


ATOM
2117
CG2
ILE
A
261
63.742
−9.071
6.120
1.00
29.08
A
C


ATOM
2118
C
ILE
A
261
60.823
−9.381
5.821
1.00
23.39
A
C


ATOM
2119
O
ILE
A
261
60.986
−10.336
5.094
1.00
21.56
A
O


ATOM
2120
N
GLU
A
262
60.037
−9.405
6.891
1.00
24.38
A
N


ATOM
2121
CA
GLU
A
262
59.268
−10.553
7.244
1.00
26.84
A
C


ATOM
2122
CB
GLU
A
262
58.520
−10.319
8.555
1.00
31.21
A
C


ATOM
2123
CG
GLU
A
262
59.428
−10.144
9.758
1.00
43.65
A
C


ATOM
2124
CD
GLU
A
262
59.708
−8.674
10.172
1.00
56.28
A
C


ATOM
2125
OE1
GLU
A
262
59.880
−7.742
9.317
1.00
45.34
A
O


ATOM
2126
OE2
GLU
A
262
59.791
−8.473
11.403
1.00
60.11
A
O


ATOM
2127
C
GLU
A
262
58.294
−10.922
6.133
1.00
21.33
A
C


ATOM
2128
O
GLU
A
262
58.098
−12.110
5.869
1.00
21.38
A
O


ATOM
2129
N
SER
A
263
57.663
−9.929
5.486
1.00
24.13
A
N


ATOM
2130
CA
ASER
A
263
56.702
−10.147
4.379
0.50
21.74
A
C


ATOM
2131
CA
BSER
A
263
56.676
−10.253
4.462
0.50
22.97
A
C


ATOM
2132
CB
ASER
A
263
56.159
−8.799
3.850
0.50
19.44
A
C


ATOM
2133
CB
BSER
A
263
55.781
−9.035
4.163
0.50
23.11
A
C


ATOM
2134
OG
ASER
A
263
57.138
−8.090
3.067
0.50
18.88
A
O


ATOM
2135
OG
BSER
A
263
54.803
−8.878
5.208
0.50
26.26
A
O


ATOM
2136
C
SER
A
263
57.380
−10.850
3.218
1.00
20.26
A
C


ATOM
2137
O
SER
A
263
56.815
−11.700
2.517
1.00
20.01
A
O


ATOM
2138
N
PHE
A
264
58.615
−10.469
2.992
1.00
21.57
A
N


ATOM
2139
CA
PHE
A
264
59.363
−11.043
1.893
1.00
20.69
A
C


ATOM
2140
CB
PHE
A
264
60.509
−10.144
1.460
1.00
27.57
A
C


ATOM
2141
CG
PHE
A
264
61.002
−10.505
0.131
1.00
25.73
A
C


ATOM
2142
CD1
PHE
A
264
60.345
−10.045
−0.985
1.00
30.05
A
C


ATOM
2143
CE1
PHE
A
264
60.746
−10.412
−2.222
1.00
33.90
A
C


ATOM
2144
CZ
PHE
A
264
61.807
−11.301
−2.357
1.00
30.18
A
C


ATOM
2145
CE2
PHE
A
264
62.447
−11.790
−1.254
1.00
31.62
A
C


ATOM
2146
CD2
PHE
A
264
62.033
−11.396
−0.004
1.00
33.82
A
C


ATOM
2147
C
PHE
A
264
59.893
−12.452
2.214
1.00
19.81
A
C


ATOM
2148
O
PHE
A
264
59.892
−13.336
1.362
1.00
23.69
A
O


ATOM
2149
N
TYR
A
265
60.344
−12.657
3.440
1.00
23.30
A
N


ATOM
2150
CA
TYR
A
265
60.692
−13.991
3.917
1.00
21.15
A
C


ATOM
2151
CB
TYR
A
265
61.113
−13.875
5.379
1.00
24.47
A
C


ATOM
2152
CG
TYR
A
265
61.316
−15.199
6.068
1.00
26.97
A
C


ATOM
2153
CD1
TYR
A
265
62.466
−15.948
5.852
1.00
38.07
A
C


ATOM
2154
CE1
TYR
A
265
62.644
−17.174
6.483
1.00
40.87
A
C


ATOM
2155
CZ
TYR
A
265
61.671
−17.636
7.336
1.00
35.57
A
C


ATOM
2156
OH
TYR
A
265
61.837
−18.829
7.985
1.00
38.87
A
O


ATOM
2157
CE2
TYR
A
265
60.544
−16.894
7.575
1.00
29.52
A
C


ATOM
2158
CD2
TYR
A
265
60.371
−15.691
6.953
1.00
27.61
A
C


ATOM
2159
C
TYR
A
265
59.506
−14.945
3.768
1.00
22.73
A
C


ATOM
2160
O
TYR
A
265
59.646
−16.089
3.312
1.00
19.72
A
O


ATOM
2161
N
TRP
A
266
58.326
−14.464
4.132
1.00
19.94
A
N


ATOM
2162
CA
TRP
A
266
57.101
−15.244
3.958
1.00
18.79
A
C


ATOM
2163
CB
TRP
A
266
55.954
−14.403
4.500
1.00
17.85
A
C


ATOM
2164
CG
TRP
A
266
54.610
−15.023
4.318
1.00
17.80
A
C


ATOM
2165
CD1
TRP
A
266
53.758
−14.885
3.230
1.00
22.77
A
C


ATOM
2166
NE1
TRP
A
266
52.624
−15.582
3.427
1.00
22.25
A
N


ATOM
2167
CE2
TRP
A
266
52.650
−16.228
4.610
1.00
20.79
A
C


ATOM
2168
CD2
TRP
A
266
53.906
−15.892
5.247
1.00
19.11
A
C


ATOM
2169
CE3
TRP
A
266
54.170
−16.384
6.507
1.00
22.12
A
C


ATOM
2170
CZ3
TRP
A
266
53.229
−17.194
7.111
1.00
22.16
A
C


ATOM
2171
CH2
TRP
A
266
52.026
−17.501
6.489
1.00
20.81
A
C


ATOM
2172
CZ2
TRP
A
266
51.703
−17.005
5.233
1.00
22.45
A
C


ATOM
2173
C
TRP
A
266
56.886
−15.615
2.506
1.00
20.46
A
C


ATOM
2174
O
TRP
A
266
56.628
−16.777
2.161
1.00
19.38
A
O


ATOM
2175
N
ALA
A
267
56.986
−14.624
1.619
1.00
18.01
A
N


ATOM
2176
CA
ALA
A
267
56.777
−14.838
0.180
1.00
19.40
A
C


ATOM
2177
CB
ALA
A
267
56.944
−13.538
−0.599
1.00
18.97
A
C


ATOM
2178
C
ALA
A
267
57.724
−15.896
−0.418
1.00
18.10
A
C


ATOM
2179
O
ALA
A
267
57.323
−16.629
−1.322
1.00
17.66
A
O


ATOM
2180
N
VAL
A
268
58.968
−15.919
0.052
1.00
20.08
A
N


ATOM
2181
CA
VAL
A
268
59.952
−16.916
−0.395
1.00
20.64
A
C


ATOM
2182
CB
VAL
A
268
61.320
−16.651
0.233
1.00
19.24
A
C


ATOM
2183
CG1
VAL
A
268
62.249
−17.869
0.121
1.00
18.95
A
C


ATOM
2184
CG2
VAL
A
268
61.966
−15.357
−0.408
1.00
18.33
A
C


ATOM
2185
C
VAL
A
268
59.485
−18.357
−0.107
1.00
22.76
A
C


ATOM
2186
O
VAL
A
268
59.676
−19.255
−0.930
1.00
19.44
A
O


ATOM
2187
N
GLY
A
269
58.846
−18.559
1.040
1.00
18.03
A
N


ATOM
2188
CA
GLY
A
269
58.269
−19.859
1.372
1.00
18.75
A
C


ATOM
2189
C
GLY
A
269
57.169
−20.244
0.425
1.00
21.80
A
C


ATOM
2190
O
GLY
A
269
57.007
−21.425
0.116
1.00
19.54
A
O


ATOM
2191
N
VAL
A
270
56.394
−19.253
−0.028
1.00
18.94
A
N


ATOM
2192
CA
VAL
A
270
55.251
−19.515
−0.892
1.00
18.32
A
C


ATOM
2193
CB
VAL
A
270
54.263
−18.320
−0.863
1.00
18.18
A
C


ATOM
2194
CG1
VAL
A
270
53.186
−18.527
−1.904
1.00
21.04
A
C


ATOM
2195
CG2
VAL
A
270
53.681
−18.132
0.574
1.00
17.70
A
C


ATOM
2196
C
VAL
A
270
55.721
−19.838
−2.329
1.00
21.10
A
C


ATOM
2197
O
VAL
A
270
55.186
−20.733
−2.986
1.00
21.08
A
O


ATOM
2198
N
ALA
A
271
56.735
−19.122
−2.813
1.00
20.64
A
N


ATOM
2199
CA
ALA
A
271
57.201
−19.324
−4.187
1.00
21.72
A
C


ATOM
2200
CB
ALA
A
271
56.412
−18.390
−5.159
1.00
26.52
A
C


ATOM
2201
C
ALA
A
271
58.681
−19.052
−4.273
1.00
21.93
A
C


ATOM
2202
O
ALA
A
271
59.111
−17.880
−4.411
1.00
18.81
A
O


ATOM
2203
N
PHE
A
272
59.494
−20.110
−4.217
1.00
16.10
A
N


ATOM
2204
CA
PHE
A
272
60.938
−19.943
−4.004
1.00
19.48
A
C


ATOM
2205
CB
PHE
A
272
61.488
−21.031
−3.094
1.00
22.36
A
C


ATOM
2206
CG
PHE
A
272
61.585
−22.349
−3.777
1.00
25.44
A
C


ATOM
2207
CD1
PHE
A
272
60.505
−23.180
−3.805
1.00
27.84
A
C


ATOM
2208
CE1
PHE
A
272
60.566
−24.387
−4.467
1.00
35.48
A
C


ATOM
2209
CZ
PHE
A
272
61.720
−24.743
−5.130
1.00
27.50
A
C


ATOM
2210
CE2
PHE
A
272
62.786
−23.919
−5.148
1.00
36.02
A
C


ATOM
2211
CD2
PHE
A
272
62.725
−22.699
−4.473
1.00
37.34
A
C


ATOM
2212
C
PHE
A
272
61.736
−19.935
−5.293
1.00
19.74
A
C


ATOM
2213
O
PHE
A
272
62.899
−19.444
−5.304
1.00
21.41
A
O


ATOM
2214
N
GLU
A
273
61.160
−20.529
−6.354
1.00
22.36
A
N


ATOM
2215
CA
GLU
A
273
61.903
−20.746
−7.597
1.00
23.02
A
C


ATOM
2216
CB
GLU
A
273
61.141
−21.571
−8.632
1.00
24.76
A
C


ATOM
2217
CG
GLU
A
273
60.815
−23.024
−8.243
1.00
29.20
A
C


ATOM
2218
CD
GLU
A
273
59.438
−23.174
−7.538
1.00
37.88
A
C


ATOM
2219
OE1
GLU
A
273
58.927
−22.222
−6.837
1.00
30.65
A
O


ATOM
2220
OE2
GLU
A
273
58.863
−24.270
−7.715
1.00
35.51
A
O


ATOM
2221
C
GLU
A
273
62.257
−19.387
−8.215
1.00
27.39
A
C


ATOM
2222
O
GLU
A
273
61.493
−18.426
−8.098
1.00
21.35
A
O


ATOM
2223
N
PRO
A
274
63.424
−19.301
−8.882
1.00
25.26
A
N


ATOM
2224
CA
PRO
A
274
63.913
−17.956
−9.207
1.00
20.90
A
C


ATOM
2225
CB
PRO
A
274
65.328
−18.201
−9.821
1.00
29.66
A
C


ATOM
2226
CG
PRO
A
274
65.441
−19.705
−10.051
1.00
24.11
A
C


ATOM
2227
CD
PRO
A
274
64.454
−20.343
−9.068
1.00
23.74
A
C


ATOM
2228
C
PRO
A
274
63.011
−17.206
−10.167
1.00
23.99
A
C


ATOM
2229
O
PRO
A
274
62.883
−15.991
−10.057
1.00
29.15
A
O


ATOM
2230
N
GLN
A
275
62.330
−17.922
−11.044
1.00
20.49
A
N


ATOM
2231
CA
GLN
A
275
61.447
−17.297
−12.028
1.00
23.59
A
C


ATOM
2232
CB
GLN
A
275
61.043
−18.339
−13.086
1.00
24.38
A
C


ATOM
2233
CG
GLN
A
275
60.258
−19.538
−12.540
1.00
28.37
A
C


ATOM
2234
CD
GLN
A
275
61.124
−20.740
−12.111
1.00
30.42
A
C


ATOM
2235
OE1
GLN
A
275
62.284
−20.608
−11.701
1.00
30.37
A
O


ATOM
2236
NE2
GLN
A
275
60.550
−21.918
−12.210
1.00
32.67
A
N


ATOM
2237
C
GLN
A
275
60.154
−16.683
−11.472
1.00
27.96
A
C


ATOM
2238
O
GLN
A
275
59.421
−16.047
−12.220
1.00
29.78
A
O


ATOM
2239
N
TYR
A
276
59.843
−16.922
−10.196
1.00
25.94
A
N


ATOM
2240
CA
TYR
A
276
58.638
−16.385
−9.573
1.00
23.53
A
C


ATOM
2241
CB
TYR
A
276
58.012
−17.459
−8.649
1.00
25.20
A
C


ATOM
2242
CG
TYR
A
276
57.475
−18.666
−9.375
1.00
29.20
A
C


ATOM
2243
CD1
TYR
A
276
56.704
−18.530
−10.528
1.00
33.14
A
C


ATOM
2244
CE1
TYR
A
276
56.204
−19.650
−11.186
1.00
38.24
A
C


ATOM
2245
CZ
TYR
A
276
56.448
−20.919
−10.668
1.00
36.54
A
C


ATOM
2246
OH
TYR
A
276
55.940
−21.987
−11.312
1.00
42.13
A
O


ATOM
2247
CE2
TYR
A
276
57.195
−21.088
−9.526
1.00
34.95
A
C


ATOM
2248
CD2
TYR
A
276
57.708
−19.957
−8.882
1.00
31.58
A
C


ATOM
2249
C
TYR
A
276
58.834
−15.077
−8.782
1.00
23.42
A
C


ATOM
2250
O
TYR
A
276
58.213
−14.873
−7.730
1.00
21.75
A
O


ATOM
2251
N
SER
A
277
59.631
−14.170
−9.318
1.00
23.32
A
N


ATOM
2252
CA
SER
A
277
59.805
−12.845
−8.747
1.00
23.74
A
C


ATOM
2253
CB
SER
A
277
60.778
−11.992
−9.604
1.00
27.94
A
C


ATOM
2254
OG
SER
A
277
62.123
−12.238
−9.199
1.00
32.61
A
O


ATOM
2255
C
SER
A
277
58.475
−12.122
−8.580
1.00
22.51
A
C


ATOM
2256
O
SER
A
277
58.199
−11.517
−7.528
1.00
19.90
A
O


ATOM
2257
N
ASP
A
278
57.643
−12.180
−9.608
1.00
20.00
A
N


ATOM
2258
CA
ASP
A
278
56.383
−11.459
−9.599
1.00
24.90
A
C


ATOM
2259
CB
ASP
A
278
55.651
−11.604
−10.926
1.00
27.70
A
C


ATOM
2260
CG
ASP
A
278
56.296
−10.798
−12.041
1.00
31.73
A
C


ATOM
2261
OD1
ASP
A
278
57.159
−9.936
−11.765
1.00
42.97
A
O


ATOM
2262
OD2
ASP
A
278
55.939
−11.050
−13.205
1.00
36.86
A
O


ATOM
2263
C
ASP
A
278
55.471
−11.953
−8.504
1.00
25.59
A
C


ATOM
2264
O
ASP
A
278
54.816
−11.148
−7.825
1.00
22.68
A
O


ATOM
2265
N
CYS
A
279
55.405
−13.272
−8.344
1.00
22.18
A
N


ATOM
2266
CA
CYS
A
279
54.622
−13.844
−7.273
1.00
21.60
A
C


ATOM
2267
CB
CYS
A
279
54.597
−15.388
−7.344
1.00
19.92
A
C


ATOM
2268
SG
CYS
A
279
53.431
−16.131
−6.153
1.00
23.77
A
S


ATOM
2269
C
CYS
A
279
55.135
−13.390
−5.924
1.00
20.82
A
C


ATOM
2270
O
CYS
A
279
54.342
−12.991
−5.083
1.00
21.23
A
O


ATOM
2271
N
ARG
A
280
56.447
−13.452
−5.698
1.00
18.39
A
N


ATOM
2272
CA
ARG
A
280
57.012
−13.025
−4.415
1.00
20.79
A
C


ATOM
2273
CB
ARG
A
280
58.523
−13.245
−4.374
1.00
20.57
A
C


ATOM
2274
CG
ARG
A
280
58.951
−14.720
−4.243
1.00
19.51
A
C


ATOM
2275
CD
ARG
A
280
60.433
−14.790
−4.036
1.00
18.20
A
C


ATOM
2276
NE
ARG
A
280
61.198
−14.498
−5.229
1.00
18.16
A
N


ATOM
2277
CZ
ARG
A
280
61.384
−15.345
−6.235
1.00
19.97
A
C


ATOM
2278
NH1
ARG
A
280
60.834
−16.552
−6.227
1.00
22.51
A
N


ATOM
2279
NH2
ARG
A
280
62.147
−14.994
−7.256
1.00
24.20
A
N


ATOM
2280
C
ARG
A
280
56.745
−11.537
−4.141
1.00
21.71
A
C


ATOM
2281
O
ARG
A
280
56.348
−11.178
−3.055
1.00
19.91
A
O


ATOM
2282
N
ASN
A
281
56.950
−10.700
−5.144
1.00
21.08
A
N


ATOM
2283
CA
ASN
A
281
56.628
−9.282
−5.027
1.00
21.89
A
C


ATOM
2284
CB
ASN
A
281
57.088
−8.530
−6.269
1.00
26.68
A
C


ATOM
2285
CG
ASN
A
281
58.615
−8.479
−6.380
1.00
36.64
A
C


ATOM
2286
OD1
ASN
A
281
59.339
−8.753
−5.410
1.00
35.43
A
O


ATOM
2287
ND2
ASN
A
281
59.107
−8.145
−7.567
1.00
33.86
A
N


ATOM
2288
C
ASN
A
281
55.156
−9.001
−4.704
1.00
21.55
A
C


ATOM
2289
O
ASN
A
281
54.874
−8.236
−3.782
1.00
21.28
A
O


ATOM
2290
N
SER
A
282
54.220
−9.669
−5.387
1.00
20.26
A
N


ATOM
2291
CA
SER
A
282
52.802
−9.522
−5.085
1.00
23.60
A
C


ATOM
2292
CB
SER
A
282
51.946
−10.354
−6.043
1.00
26.14
A
C


ATOM
2293
OG
SER
A
282
51.551
−9.582
−7.129
1.00
31.01
A
O


ATOM
2294
C
SER
A
282
52.448
−9.961
−3.678
1.00
21.74
A
C


ATOM
2295
O
SER
A
282
51.746
−9.270
−2.964
1.00
20.53
A
O


ATOM
2296
N
VAL
A
283
52.895
−11.146
−3.302
1.00
20.71
A
N


ATOM
2297
CA
VAL
A
283
52.621
−11.663
−1.974
1.00
17.85
A
C


ATOM
2298
CB
VAL
A
283
53.055
−13.132
−1.855
1.00
20.08
A
C


ATOM
2299
CG1
VAL
A
283
52.977
−13.611
−0.395
1.00
19.46
A
C


ATOM
2300
CG2
VAL
A
283
52.150
−13.967
−2.709
1.00
19.85
A
C


ATOM
2301
C
VAL
A
283
53.198
−10.775
−0.891
1.00
17.07
A
C


ATOM
2302
O
VAL
A
283
52.506
−10.479
0.094
1.00
16.85
A
O


ATOM
2303
N
ALA
A
284
54.434
−10.306
−1.059
1.00
15.81
A
N


ATOM
2304
CA
ALA
A
284
55.050
−9.476
−0.028
1.00
17.39
A
C


ATOM
2305
CB
ALA
A
284
56.522
−9.255
−0.317
1.00
17.17
A
C


ATOM
2306
C
ALA
A
284
54.301
−8.119
0.088
1.00
17.57
A
C


ATOM
2307
O
ALA
A
284
54.009
−7.625
1.216
1.00
17.20
A
O


ATOM
2308
N
LYS
A
285
53.977
−7.511
−1.058
1.00
17.42
A
N


ATOM
2309
CA
LYS
A
285
53.155
−6.272
−1.062
1.00
18.85
A
C


ATOM
2310
CB
LYS
A
285
52.884
−5.789
−2.487
1.00
18.52
A
C


ATOM
2311
CG
LYS
A
285
54.103
−5.210
−3.181
1.00
19.50
A
C


ATOM
2312
CD
LYS
A
285
53.771
−4.777
−4.622
1.00
24.80
A
C


ATOM
2313
CE
LYS
A
285
54.911
−3.939
−5.170
1.00
29.57
A
C


ATOM
2314
NZ
LYS
A
285
54.911
−3.908
−6.624
1.00
45.89
A
N


ATOM
2315
C
LYS
A
285
51.805
−6.474
−0.329
1.00
19.27
A
C


ATOM
2316
O
LYS
A
285
51.427
−5.669
0.529
1.00
19.71
A
O


ATOM
2317
N
MET
A
286
51.080
−7.538
−0.673
1.00
17.23
A
N


ATOM
2318
CA
MET
A
286
49.811
−7.824
−0.014
1.00
18.12
A
C


ATOM
2319
CB
MET
A
286
49.107
−9.038
−0.597
1.00
21.82
A
C


ATOM
2320
CG
MET
A
286
48.651
−8.866
−2.034
1.00
22.74
A
C


ATOM
2321
SD
MET
A
286
47.766
−7.321
−2.298
1.00
26.21
A
S


ATOM
2322
CE
MET
A
286
48.995
−6.119
−2.880
1.00
18.48
A
C


ATOM
2323
C
MET
A
286
50.008
−8.024
1.486
1.00
18.96
A
C


ATOM
2324
O
MET
A
286
49.244
−7.494
2.316
1.00
22.71
A
O


ATOM
2325
N
PHE
A
287
51.039
−8.764
1.849
1.00
19.84
A
N


ATOM
2326
CA
PHE
A
287
51.292
−8.955
3.279
1.00
23.52
A
C


ATOM
2327
CB
PHE
A
287
52.377
−9.994
3.603
1.00
29.12
A
C


ATOM
2328
CG
PHE
A
287
51.983
−10.939
4.745
1.00
42.44
A
C


ATOM
2329
CD1
PHE
A
287
50.761
−10.768
5.464
1.00
62.09
A
C


ATOM
2330
CE1
PHE
A
287
50.368
−11.651
6.509
1.00
52.92
A
C


ATOM
2331
CZ
PHE
A
287
51.186
−12.705
6.838
1.00
49.40
A
C


ATOM
2332
CE2
PHE
A
287
52.409
−12.884
6.145
1.00
59.69
A
C


ATOM
2333
CD2
PHE
A
287
52.804
−11.993
5.110
1.00
42.43
A
C


ATOM
2334
C
PHE
A
287
51.590
−7.700
4.029
1.00
21.35
A
C


ATOM
2335
O
PHE
A
287
51.140
−7.560
5.167
1.00
20.18
A
O


ATOM
2336
N
CYS
A
288
52.300
−6.760
3.395
1.00
19.81
A
N


ATOM
2337
CA
CYS
A
288
52.546
−5.464
4.016
1.00
20.01
A
C


ATOM
2338
CB
CYS
A
288
53.430
−4.568
3.152
1.00
24.96
A
C


ATOM
2339
SG
CYS
A
288
55.186
−5.053
3.151
1.00
27.36
A
S


ATOM
2340
C
CYS
A
288
51.265
−4.720
4.304
1.00
21.13
A
C


ATOM
2341
O
CYS
A
288
51.118
−4.110
5.367
1.00
19.04
A
O


ATOM
2342
N
PHE
A
289
50.353
−4.719
3.340
1.00
16.53
A
N


ATOM
2343
CA
PHE
A
289
49.049
−4.074
3.552
1.00
16.27
A
C


ATOM
2344
CB
PHE
A
289
48.312
−3.898
2.239
1.00
15.49
A
C


ATOM
2345
CG
PHE
A
289
48.736
−2.675
1.491
1.00
16.61
A
C


ATOM
2346
CD1
PHE
A
289
48.413
−1.421
1.957
1.00
18.72
A
C


ATOM
2347
CE1
PHE
A
289
48.818
−0.296
1.269
1.00
18.70
A
C


ATOM
2348
CZ
PHE
A
289
49.543
−0.424
0.109
1.00
18.93
A
C


ATOM
2473
CG
ASP
A
304
30.689
4.873
14.493
1.00
35.20
A
C


ATOM
2474
OD1
ASP
A
304
29.830
3.981
14.300
1.00
31.47
A
O


ATOM
2475
OD2
ASP
A
304
30.547
5.751
15.375
1.00
38.27
A
O


ATOM
2476
C
ASP
A
304
33.353
3.814
11.863
1.00
20.30
A
C


ATOM
2477
O
ASP
A
304
32.998
3.846
10.683
1.00
17.82
A
O


ATOM
2478
N
GLU
A
305
34.626
3.993
12.212
1.00
15.41
A
N


ATOM
2479
CA
GLU
A
305
35.650
4.171
11.182
1.00
14.29
A
C


ATOM
2480
CB
GLU
A
305
37.008
4.559
11.816
1.00
15.22
A
C


ATOM
2481
CG
GLU
A
305
37.032
5.928
12.462
1.00
17.43
A
C


ATOM
2482
CD
GLU
A
305
38.312
6.178
13.263
1.00
19.71
A
C


ATOM
2483
OE1
GLU
A
305
38.970
5.222
13.736
1.00
17.09
A
O


ATOM
2484
OE2
GLU
A
305
38.691
7.339
13.399
1.00
20.00
A
O


ATOM
2485
C
GLU
A
305
35.819
2.918
10.345
1.00
14.09
A
C


ATOM
2486
O
GLU
A
305
35.994
2.971
9.109
1.00
14.06
A
O


ATOM
2487
N
LEU
A
306
35.788
1.764
11.006
1.00
13.12
A
N


ATOM
2488
CA
LEU
A
306
36.012
0.521
10.306
1.00
12.82
A
C


ATOM
2489
CB
LEU
A
306
36.129
−0.638
11.315
1.00
12.26
A
C


ATOM
2490
CG
LEU
A
306
37.275
−0.474
12.341
1.00
13.73
A
C


ATOM
2491
CD1
LEU
A
306
37.292
−1.686
13.329
1.00
14.11
A
C


ATOM
2492
CD2
LEU
A
306
38.622
−0.339
11.638
1.00
11.07
A
C


ATOM
2493
C
LEU
A
306
34.864
0.281
9.314
1.00
15.39
A
C


ATOM
2494
O
LEU
A
306
35.069
−0.300
8.262
1.00
16.92
A
O


ATOM
2495
N
GLU
A
307
33.649
0.700
9.678
1.00
16.87
A
N


ATOM
2496
CA
GLU
A
307
32.506
0.557
8.770
1.00
17.68
A
C


ATOM
2497
CB
GLU
A
307
31.207
0.986
9.444
1.00
20.36
A
C


ATOM
2498
CG
GLU
A
307
30.719
0.021
10.484
1.00
28.93
A
C


ATOM
2499
CD
GLU
A
307
29.932
−1.139
9.887
1.00
37.37
A
C


ATOM
2500
OE1
GLU
A
307
29.757
−1.178
8.640
1.00
38.07
A
O


ATOM
2501
OE2
GLU
A
307
29.481
−2.002
10.674
1.00
38.86
A
O


ATOM
2502
C
GLU
A
307
32.759
1.393
7.529
1.00
17.79
A
C


ATOM
2503
O
GLU
A
307
32.521
0.925
6.416
1.00
18.41
A
O


ATOM
2504
N
LEU
A
308
33.305
2.599
7.709
1.00
16.45
A
N


ATOM
2505
CA
LEU
A
308
33.621
3.481
6.560
1.00
18.98
A
C


ATOM
2506
CB
LEU
A
308
34.068
4.869
7.014
1.00
18.90
A
C


ATOM
2507
CG
LEU
A
308
33.028
5.811
7.618
1.00
28.08
A
C


ATOM
2508
CD1
LEU
A
308
33.737
7.021
8.304
1.00
26.55
A
C


ATOM
2509
CD2
LEU
A
308
32.061
6.267
6.534
1.00
26.63
A
C


ATOM
2510
C
LEU
A
308
34.702
2.892
5.668
1.00
18.24
A
C


ATOM
2511
O
LEU
A
308
34.626
2.940
4.436
1.00
17.72
A
O


ATOM
2512
N
PHE
A
309
35.730
2.329
6.290
1.00
18.59
A
N


ATOM
2513
CA
PHE
A
309
36.816
1.768
5.506
1.00
16.74
A
C


ATOM
2514
CB
PHE
A
309
37.991
1.339
6.397
1.00
14.25
A
C


ATOM
2515
CG
PHE
A
309
39.246
1.079
5.632
1.00
13.41
A
C


ATOM
2516
CD1
PHE
A
309
40.125
2.098
5.370
1.00
15.08
A
C


ATOM
2517
CE1
PHE
A
309
41.275
1.879
4.720
1.00
15.28
A
C


ATOM
2518
CZ
PHE
A
309
41.573
0.638
4.260
1.00
16.06
A
C


ATOM
2519
CE2
PHE
A
309
40.706
−0.399
4.491
1.00
16.55
A
C


ATOM
2520
CD2
PHE
A
309
39.564
−0.185
5.206
1.00
14.73
A
C


ATOM
2521
C
PHE
A
309
36.339
0.612
4.679
1.00
17.02
A
C


ATOM
2522
O
PHE
A
309
36.698
0.499
3.508
1.00
16.41
A
O


ATOM
2523
N
THR
A
310
35.567
−0.273
5.312
1.00
17.03
A
N


ATOM
2524
CA
THR
A
310
35.042
−1.481
4.644
1.00
15.57
A
C


ATOM
2525
CB
THR
A
310
34.254
−2.379
5.611
1.00
16.94
A
C


ATOM
2526
OG1
THR
A
310
35.064
−2.617
6.764
1.00
17.27
A
O


ATOM
2527
CG2
THR
A
310
33.868
−3.727
4.976
1.00
14.06
A
C


ATOM
2528
C
THR
A
310
34.165
−1.121
3.464
1.00
14.32
A
C


ATOM
2529
O
THR
A
310
34.320
−1.695
2.395
1.00
14.04
A
O


ATOM
2530
N
ASP
A
311
33.312
−0.119
3.647
1.00
16.34
A
N


ATOM
2531
CA
ASP
A
311
32.457
0.399
2.571
1.00
19.21
A
C


ATOM
2532
CB
ASP
A
311
31.456
1.401
3.158
1.00
21.06
A
C


ATOM
2533
CG
ASP
A
311
30.602
2.056
2.106
1.00
25.80
A
C


ATOM
2534
OD1
ASP
A
311
29.749
1.365
1.516
1.00
27.76
A
O


ATOM
2535
OD2
ASP
A
311
30.805
3.261
1.851
1.00
29.96
A
O


ATOM
2536
C
ASP
A
311
33.277
1.007
1.420
1.00
19.30
A
C


ATOM
2537
O
ASP
A
311
32.964
0.797
0.251
1.00
17.59
A
O


ATOM
2538
N
ALA
A
312
34.345
1.724
1.755
1.00
16.06
A
N


ATOM
2539
CA
ALA
A
312
35.227
2.322
0.751
1.00
16.73
A
C


ATOM
2540
CB
ALA
A
312
36.273
3.210
1.412
1.00
14.46
A
C


ATOM
2541
C
ALA
A
312
35.897
1.270
−0.109
1.00
16.20
A
C


ATOM
2542
O
ALA
A
312
35.982
1.436
−1.330
1.00
16.25
A
O


ATOM
2543
N
VAL
A
313
36.369
0.187
0.518
1.00
15.49
A
N


ATOM
2544
CA
VAL
A
313
36.957
−0.925
−0.200
1.00
15.70
A
C


ATOM
2545
CB
VAL
A
313
37.658
−1.919
0.749
1.00
15.23
A
C


ATOM
2546
CG1
VAL
A
313
38.040
−3.196
0.026
1.00
13.55
A
C


ATOM
2547
CG2
VAL
A
313
38.910
−1.242
1.389
1.00
15.29
A
C


ATOM
2548
C
VAL
A
313
35.928
−1.637
−1.069
1.00
16.66
A
C


ATOM
2549
O
VAL
A
313
36.216
−1.976
−2.236
1.00
14.48
A
O


ATOM
2550
N
GLU
A
314
34.744
−1.861
−0.528
1.00
17.83
A
N


ATOM
2551
CA
GLU
A
314
33.635
−2.427
−1.327
1.00
18.81
A
C


ATOM
2552
CB
GLU
A
314
32.376
−2.598
−0.478
1.00
21.15
A
C


ATOM
2553
CG
GLU
A
314
32.502
−3.730
0.572
1.00
26.43
A
C


ATOM
2554
CD
GLU
A
314
31.275
−3.846
1.486
1.00
29.94
A
C


ATOM
2555
OE1
GLU
A
314
30.460
−2.909
1.524
1.00
31.98
A
O


ATOM
2556
OE2
GLU
A
314
31.135
−4.858
2.187
1.00
28.42
A
O


ATOM
2557
C
GLU
A
314
33.297
−1.593
−2.539
1.00
18.03
A
C


ATOM
2558
O
GLU
A
314
33.116
−2.129
−3.610
1.00
20.81
A
O


ATOM
2559
N
ARG
A
315
33.226
−0.289
−2.389
1.00
15.47
A
N


ATOM
2560
CA
AARG
A
315
32.841
0.611
−3.487
0.50
18.54
A
C


ATOM
2561
CA
BARG
A
315
32.839
0.563
−3.515
0.50
19.14
A
C


ATOM
2562
CB
AARG
A
315
32.383
1.976
−2.940
0.50
18.77
A
C


ATOM
2563
CB
BARG
A
315
32.243
1.871
−3.013
0.50
20.09
A
C


ATOM
2564
CG
AARG
A
315
31.037
1.960
−2.161
0.50
21.10
A
C


ATOM
2565
CG
BARG
A
315
30.830
1.674
−2.471
0.50
23.70
A
C


ATOM
2566
CD
AARG
A
315
30.504
3.375
−1.817
0.50
21.65
A
C


ATOM
2567
CD
BARG
A
315
30.324
2.865
−1.681
0.50
24.91
A
C


ATOM
2568
NE
AARG
A
315
31.132
3.980
−0.634
0.50
23.68
A
N


ATOM
2569
NE
BARG
A
315
29.008
2.591
−1.126
0.50
29.79
A
N


ATOM
2570
CZ
AARG
A
315
32.199
4.775
−0.664
0.50
20.63
A
C


ATOM
2571
CZ
BARG
A
315
27.887
2.612
−1.837
0.50
36.61
A
C


ATOM
2572
NH1
AARG
A
315
32.691
5.268
0.460
0.50
23.51
A
N


ATOM
2573
NH1
BARG
A
315
27.927
2.897
−3.129
0.50
38.35
A
N


ATOM
2574
NH2
BARG
A
315
26.727
2.345
−1.259
0.50
38.64
A
N


ATOM
2575
NH2
AARG
A
315
32.780
5.075
−1.811
0.50
19.97
A
N


ATOM
2576
C
ARG
A
315
33.991
0.809
−4.497
1.00
21.60
A
C


ATOM
2577
O
ARG
A
315
33.767
1.030
−5.674
1.00
16.65
A
O


ATOM
2578
N
TRP
A
316
35.229
0.771
−4.018
1.00
17.56
A
N


ATOM
2579
CA
TRP
A
316
36.390
0.927
−4.889
1.00
14.98
A
C


ATOM
2580
CB
TRP
A
316
36.592
−0.288
−5.758
1.00
16.79
A
C


ATOM
2581
CG
TRP
A
316
38.049
−0.433
−6.158
1.00
17.07
A
C


ATOM
2582
CD1
TRP
A
316
38.638
−0.174
−7.390
1.00
14.99
A
C


ATOM
2583
NE1
TRP
A
316
39.991
−0.401
−7.336
1.00
14.25
A
N


ATOM
2584
CE2
TRP
A
316
40.345
−0.805
−6.118
1.00
12.86
A
C


ATOM
2585
CD2
TRP
A
316
39.135
−0.831
−5.299
1.00
13.85
A
C


ATOM
2586
CE3
TRP
A
316
39.226
−1.236
−3.987
1.00
15.44
A
C


ATOM
2587
CZ3
TRP
A
316
40.479
−1.594
−3.486
1.00
15.61
A
C


ATOM
2588
CH2
TRP
A
316
41.628
−1.540
−4.285
1.00
15.25
A
C


ATOM
2589
CZ2
TRP
A
316
41.575
−1.143
−5.609
1.00
14.44
A
C


ATOM
2590
C
TRP
A
316
36.276
2.163
−5.712
1.00
14.89
A
C


ATOM
2591
O
TRP
A
316
36.419
2.143
−6.917
1.00
15.76
A
O


ATOM
2592
N
ASP
A
317
36.032
3.262
−5.035
1.00
16.96
A
N


ATOM
2593
CA
ASP
A
317
35.800
4.537
−5.687
1.00
18.34
A
C


ATOM
2594
CB
ASP
A
317
34.358
4.911
−5.441
1.00
18.08
A
C


ATOM
2595
CG
ASP
A
317
33.984
6.287
−6.000
1.00
24.63
A
C


ATOM
2596
OD1
ASP
A
317
34.793
6.935
−6.711
1.00
27.57
A
O


ATOM
2597
OD2
ASP
A
317
32.833
6.690
−5.740
1.00
28.48
A
O


ATOM
2598
C
ASP
A
317
36.731
5.605
−5.133
1.00
20.19
A
C


ATOM
2599
O
ASP
A
317
36.512
6.114
−4.018
1.00
20.93
A
O


ATOM
2600
N
VAL
A
318
37.711
6.006
−5.939
1.00
18.34
A
N


ATOM
2601
CA
VAL
A
318
38.710
6.991
−5.503
1.00
19.63
A
C


ATOM
2602
CB
VAL
A
318
39.861
7.145
−6.538
1.00
19.36
A
C


ATOM
2603
CG1
VAL
A
318
39.498
8.102
−7.663
1.00
21.41
A
C


ATOM
2604
CG2
VAL
A
318
41.139
7.632
−5.874
1.00
19.12
A
C


ATOM
2605
C
VAL
A
318
38.088
8.369
−5.277
1.00
29.46
A
C


ATOM
2606
O
VAL
A
318
38.605
9.168
−4.488
1.00
29.09
A
O


ATOM
2607
N
ASN
A
319
37.018
8.664
−6.018
1.00
26.29
A
N


ATOM
2608
CA
ASN
A
319
36.374
9.983
−5.980
1.00
31.77
A
C


ATOM
2609
CB
ASN
A
319
35.483
10.228
−7.232
1.00
28.73
A
C


ATOM
2610
CG
ASN
A
319
36.280
10.184
−8.574
1.00
31.03
A
C


ATOM
2611
OD1
ASN
A
319
37.240
10.959
−8.786
1.00
25.79
A
O


ATOM
2612
ND2
ASN
A
319
35.836
9.314
−9.500
1.00
16.89
A
N


ATOM
2613
C
ASN
A
319
35.540
10.153
−4.724
1.00
27.47
A
C


ATOM
2614
O
ASN
A
319
34.901
11.182
−4.589
1.00
31.81
A
O


ATOM
2615
N
ALA
A
320
35.537
9.156
−3.832
1.00
25.56
A
N


ATOM
2616
CA
ALA
A
320
34.920
9.253
−2.474
1.00
35.70
A
C


ATOM
2617
CB
ALA
A
320
33.740
8.259
−2.370
1.00
30.73
A
C


ATOM
2618
C
ALA
A
320
35.890
9.032
−1.269
1.00
35.09
A
C


ATOM
2619
O
ALA
A
320
35.446
8.708
−0.177
1.00
42.99
A
O


ATOM
2620
N
ILE
A
321
37.204
9.178
−1.449
1.00
35.73
A
N


ATOM
2621
CA
ILE
A
321
38.153
9.042
−0.313
1.00
25.46
A
C


ATOM
2622
CB
ILE
A
321
39.658
8.885
−0.768
1.00
31.72
A
C


ATOM
2623
CG1
ILE
A
321
40.230
10.186
−1.351
1.00
35.99
A
C


ATOM
2624
CD1
ILE
A
321
41.644
10.023
−1.980
1.00
41.27
A
C


ATOM
2625
CG2
ILE
A
321
39.813
7.709
−1.779
1.00
34.61
A
C


ATOM
2626
C
ILE
A
321
38.039
10.199
0.704
1.00
39.12
A
C


ATOM
2627
O
ILE
A
321
38.426
10.049
1.867
1.00
36.11
A
O


ATOM
2628
N
ASN
A
322
37.509
11.351
0.273
1.00
32.92
A
N


ATOM
2629
CA
ASN
A
322
37.210
12.447
1.197
1.00
31.27
A
C


ATOM
2630
CB
ASN
A
322
36.903
13.743
0.404
1.00
30.22
A
C


ATOM
2631
CG
ASN
A
322
38.191
14.408
−0.185
1.00
33.53
A
C


ATOM
2632
OD1
ASN
A
322
39.337
14.000
0.108
1.00
29.13
A
O


ATOM
2633
ND2
ASN
A
322
38.000
15.458
−0.974
1.00
32.82
A
N


ATOM
2634
C
ASN
A
322
36.143
12.121
2.303
1.00
29.49
A
C


ATOM
2635
O
ASN
A
322
36.017
12.864
3.259
1.00
28.36
A
O


ATOM
2636
N
ASP
A
323
35.446
10.986
2.228
1.00
31.55
A
N


ATOM
2637
CA
ASP
A
323
34.609
10.518
3.378
1.00
36.24
A
C


ATOM
2638
CB
ASP
A
323
33.601
9.414
2.946
1.00
51.90
A
C


ATOM
2639
CG
ASP
A
323
32.960
9.644
1.559
1.00
53.60
A
C


ATOM
2640
OD1
ASP
A
323
32.937
10.788
1.060
1.00
51.28
A
O


ATOM
2641
OD2
ASP
A
323
32.468
8.645
0.970
1.00
51.14
A
O


ATOM
2642
C
ASP
A
323
35.422
9.913
4.589
1.00
30.18
A
C


ATOM
2643
O
ASP
A
323
34.897
9.759
5.680
1.00
31.04
A
O


ATOM
2644
N
LEU
A
324
36.674
9.520
4.377
1.00
24.50
A
N


ATOM
2645
CA
LEU
A
324
37.419
8.730
5.377
1.00
19.57
A
C


ATOM
2646
CB
LEU
A
324
38.435
7.858
4.653
1.00
18.49
A
C


ATOM
2647
CG
LEU
A
324
37.837
6.719
3.832
1.00
21.24
A
C


ATOM
2648
CD1
LEU
A
324
38.864
6.103
2.908
1.00
16.54
A
C


ATOM
2649
CD2
LEU
A
324
37.287
5.677
4.774
1.00
22.23
A
C


ATOM
2650
C
LEU
A
324
38.162
9.642
6.333
1.00
17.96
A
C


ATOM
2651
O
LEU
A
324
38.552
10.704
5.926
1.00
16.89
A
O


ATOM
2652
N
PRO
A
325
38.414
9.216
7.588
1.00
17.81
A
N


ATOM
2653
CA
PRO
A
325
39.338
10.029
8.402
1.00
20.53
A
C


ATOM
2654
CB
PRO
A
325
39.333
9.323
9.772
1.00
22.81
A
C


ATOM
2655
CG
PRO
A
325
38.873
7.979
9.501
1.00
21.72
A
C


ATOM
2656
CD
PRO
A
325
37.934
8.046
8.322
1.00
20.29
A
C


ATOM
2657
C
PRO
A
325
40.740
10.065
7.808
1.00
15.43
A
C


ATOM
2658
O
PRO
A
325
41.092
9.176
7.053
1.00
14.99
A
O


ATOM
2659
N
ASP
A
326
41.538
11.069
8.166
1.00
13.83
A
N


ATOM
2660
CA
ASP
A
326
42.831
11.314
7.484
1.00
16.63
A
C


ATOM
2661
CB
ASP
A
326
43.571
12.457
8.150
1.00
20.34
A
C


ATOM
2662
CG
ASP
A
326
42.919
13.819
7.867
1.00
22.99
A
C


ATOM
2663
OD1
ASP
A
326
42.066
13.906
6.956
1.00
27.29
A
O


ATOM
2664
OD2
ASP
A
326
43.277
14.778
8.567
1.00
25.92
A
O


ATOM
2665
C
ASP
A
326
43.750
10.090
7.405
1.00
18.36
A
C


ATOM
2666
O
ASP
A
326
44.271
9.757
6.326
1.00
14.64
A
O


ATOM
2667
N
TYR
A
327
43.925
9.374
8.516
1.00
16.45
A
N


ATOM
2668
CA
TYR
A
327
44.789
8.191
8.458
1.00
17.44
A
C


ATOM
2669
CB
TYR
A
327
45.028
7.627
9.845
1.00
18.71
A
C


ATOM
2670
CG
TYR
A
327
43.903
6.798
10.411
1.00
19.90
A
C


ATOM
2671
CD1
TYR
A
327
42.801
7.382
11.069
1.00
17.57
A
C


ATOM
2672
CE1
TYR
A
327
41.781
6.566
11.617
1.00
18.13
A
C


ATOM
2673
CZ
TYR
A
327
41.902
5.186
11.552
1.00
21.33
A
C


ATOM
2674
OH
TYR
A
327
40.931
4.323
12.067
1.00
20.25
A
O


ATOM
2675
CE2
TYR
A
327
42.985
4.623
10.923
1.00
20.79
A
C


ATOM
2676
CD2
TYR
A
327
43.977
5.418
10.362
1.00
20.19
A
C


ATOM
2677
C
TYR
A
327
44.273
7.116
7.496
1.00
16.83
A
C


ATOM
2678
O
TYR
A
327
45.056
6.419
6.831
1.00
18.49
A
O


ATOM
2679
N
MET
A
328
42.957
6.943
7.414
1.00
13.77
A
N


ATOM
2680
CA
MET
A
328
42.407
5.982
6.460
1.00
13.61
A
C


ATOM
2681
CB
MET
A
328
40.978
5.630
6.807
1.00
14.88
A
C


ATOM
2682
CG
MET
A
328
40.877
4.751
8.063
1.00
14.77
A
C


ATOM
2683
SD
MET
A
328
39.182
4.362
8.436
1.00
16.72
A
S


ATOM
2684
CE
MET
A
328
39.456
2.802
9.383
1.00
11.78
A
C


ATOM
2685
C
MET
A
328
42.437
6.430
4.985
1.00
16.66
A
C


ATOM
2686
O
MET
A
328
42.492
5.587
4.112
1.00
17.33
A
O


ATOM
2687
N
LYS
A
329
42.369
7.737
4.712
1.00
18.49
A
N


ATOM
2688
CA
LYS
A
329
42.474
8.244
3.329
1.00
19.66
A
C


ATOM
2689
CB
LYS
A
329
42.521
9.780
3.268
1.00
25.10
A
C


ATOM
2690
CG
LYS
A
329
41.299
10.557
3.662
1.00
39.00
A
C


ATOM
2691
CD
LYS
A
329
41.464
12.012
3.167
1.00
38.07
A
C


ATOM
2692
CE
LYS
A
329
40.322
12.928
3.565
1.00
44.10
A
C


ATOM
2693
NZ
LYS
A
329
40.381
14.163
2.715
1.00
35.97
A
N


ATOM
2694
C
LYS
A
329
43.777
7.807
2.743
1.00
15.39
A
C


ATOM
2695
O
LYS
A
329
43.840
7.348
1.612
1.00
15.48
A
O


ATOM
2696
N
LEU
A
330
44.844
7.976
3.521
1.00
13.32
A
N


ATOM
2697
CA
LEU
A
330
46.174
7.646
3.063
1.00
15.58
A
C


ATOM
2698
CB
LEU
A
330
47.214
8.193
4.060
1.00
19.08
A
C


ATOM
2699
CG
LEU
A
330
48.663
8.198
3.585
1.00
20.74
A
C


ATOM
2700
CD1
LEU
A
330
48.827
9.359
2.531
1.00
16.60
A
C


ATOM
2701
CD2
LEU
A
330
49.680
8.377
4.787
1.00
19.71
A
C


ATOM
2702
C
LEU
A
330
46.332
6.172
2.871
1.00
16.37
A
C


ATOM
2703
O
LEU
A
330
46.825
5.723
1.825
1.00
15.79
A
O


ATOM
2704
N
CYS
A
331
45.901
5.387
3.870
1.00
15.05
A
N


ATOM
2705
CA
ACYS
A
331
45.980
3.935
3.762
0.70
14.81
A
C


ATOM
2706
CA
BCYS
A
331
45.934
3.917
3.778
0.30
14.73
A
C


ATOM
2707
CB
ACYS
A
331
45.506
3.277
5.069
0.70
17.32
A
C


ATOM
2708
CB
BCYS
A
331
45.314
3.292
5.035
0.30
15.28
A
C


ATOM
2709
SG
ACYS
A
331
45.829
1.513
5.086
0.70
25.66
A
S


ATOM
2710
SG
BCYS
A
331
46.397
3.282
6.443
0.30
17.26
A
S


ATOM
2711
C
CYS
A
331
45.169
3.397
2.564
1.00
14.98
A
C


ATOM
2712
O
CYS
A
331
45.667
2.584
1.765
1.00
14.34
A
O


ATOM
2713
N
PHE
A
332
43.934
3.835
2.448
1.00
15.08
A
N


ATOM
2714
CA
PHE
A
332
43.086
3.462
1.324
1.00
15.82
A
C


ATOM
2715
CB
PHE
A
332
41.716
4.117
1.443
1.00
19.85
A
C


ATOM
2716
CG
PHE
A
332
40.816
3.771
0.300
1.00
22.23
A
C


ATOM
2717
CD1
PHE
A
332
40.144
2.592
0.302
1.00
19.24
A
C


ATOM
2718
CE1
PHE
A
332
39.323
2.244
−0.761
1.00
23.63
A
C


ATOM
2719
CZ
PHE
A
332
39.188
3.073
−1.830
1.00
17.47
A
C


ATOM
2720
CE2
PHE
A
332
39.879
4.249
−1.880
1.00
18.18
A
C


ATOM
2721
CD2
PHE
A
332
40.706
4.605
−0.811
1.00
19.50
A
C


ATOM
2722
C
PHE
A
332
43.678
3.823
−0.051
1.00
13.90
A
C


ATOM
2723
O
PHE
A
332
43.704
2.981
−0.938
1.00
13.13
A
O


ATOM
2724
N
LEU
A
333
44.182
5.038
−0.223
1.00
13.85
A
N


ATOM
2725
CA
LEU
A
333
44.677
5.452
−1.561
1.00
14.29
A
C


ATOM
2726
CB
LEU
A
333
44.865
6.948
−1.655
1.00
12.13
A
C


ATOM
2727
CG
LEU
A
333
45.367
7.557
−2.976
1.00
13.38
A
C


ATOM
2728
CD1
LEU
A
333
44.438
7.120
−4.114
1.00
12.36
A
C


ATOM
2729
CD2
LEU
A
333
45.483
9.094
−2.883
1.00
10.36
A
C


ATOM
2730
C
LEU
A
333
45.959
4.665
−1.899
1.00
14.16
A
C


ATOM
2731
O
LEU
A
333
46.157
4.224
−3.055
1.00
11.14
A
O


ATOM
2732
N
ALA
A
334
46.789
4.409
−0.883
1.00
12.87
A
N


ATOM
2733
CA
ALA
A
334
47.977
3.554
−1.090
1.00
16.15
A
C


ATOM
2734
CB
ALA
A
334
48.840
3.454
0.173
1.00
15.82
A
C


ATOM
2735
C
ALA
A
334
47.576
2.165
−1.562
1.00
13.62
A
C


ATOM
2736
O
ALA
A
334
48.135
1.663
−2.498
1.00
14.28
A
O


ATOM
2737
N
LEU
A
335
46.593
1.564
−0.924
1.00
14.24
A
N


ATOM
2738
CA
LEU
A
335
46.123
0.216
−1.302
1.00
16.91
A
C


ATOM
2739
CB
LEU
A
335
45.090
−0.277
−0.299
1.00
14.05
A
C


ATOM
2740
CG
LEU
A
335
44.334
−1.570
−0.507
1.00
19.27
A
C


ATOM
2741
CD1
LEU
A
335
45.331
−2.720
−0.481
1.00
18.50
A
C


ATOM
2742
CD2
LEU
A
335
43.181
−1.768
0.501
1.00
19.37
A
C


ATOM
2743
C
LEU
A
335
45.486
0.251
−2.686
1.00
14.42
A
C


ATOM
2744
O
LEU
A
335
45.750
−0.604
−3.523
1.00
16.54
A
O


ATOM
2745
N
TYR
A
336
44.624
1.238
−2.889
1.00
14.24
A
N


ATOM
2746
CA
TYR
A
336
43.909
1.458
−4.153
1.00
13.22
A
C


ATOM
2747
CB
TYR
A
336
43.129
2.755
−4.043
1.00
12.76
A
C


ATOM
2748
CG
TYR
A
336
42.168
3.066
−5.171
1.00
14.14
A
C


ATOM
2749
CD1
TYR
A
336
40.915
2.497
−5.220
1.00
15.26
A
C


ATOM
2750
CE1
TYR
A
336
40.032
2.790
−6.264
1.00
16.12
A
C


ATOM
2751
CZ
TYR
A
336
40.389
3.682
−7.208
1.00
17.61
A
C


ATOM
2752
OH
TYR
A
336
39.521
4.011
−8.230
1.00
19.27
A
O


ATOM
2753
CE2
TYR
A
336
41.620
4.279
−7.162
1.00
15.70
A
C


ATOM
2754
CD2
TYR
A
336
42.497
3.974
−6.154
1.00
14.82
A
C


ATOM
2755
C
TYR
A
336
44.860
1.497
−5.345
1.00
12.46
A
C


ATOM
2756
O
TYR
A
336
44.689
0.722
−6.318
1.00
11.23
A
O


ATOM
2757
N
ASN
A
337
45.867
2.376
−5.267
1.00
11.05
A
N


ATOM
2758
CA
ASN
A
337
46.913
2.477
−6.325
1.00
10.92
A
C


ATOM
2759
CB
ASN
A
337
47.864
3.662
−6.045
1.00
13.08
A
C


ATOM
2760
CG
ASN
A
337
47.178
5.011
−6.226
1.00
16.67
A
C


ATOM
2761
OD1
ASN
A
337
46.117
5.089
−6.859
1.00
14.21
A
O


ATOM
2762
ND2
ASN
A
337
47.749
6.078
−5.629
1.00
12.56
A
N


ATOM
2763
C
ASN
A
337
47.718
1.200
−6.538
1.00
13.12
A
C


ATOM
2764
O
ASN
A
337
47.994
0.825
−7.671
1.00
11.66
A
O


ATOM
2765
N
THR
A
338
48.097
0.524
−5.446
1.00
10.90
A
N


ATOM
2766
CA
THR
A
338
48.859
−0.714
−5.539
1.00
11.86
A
C


ATOM
2767
CB
THR
A
338
49.291
−1.196
−4.096
1.00
13.78
A
C


ATOM
2768
OG1
THR
A
338
50.031
−0.117
−3.493
1.00
13.95
A
O


ATOM
2769
CG2
THR
A
338
50.138
−2.535
−4.140
1.00
16.03
A
C


ATOM
2770
C
THR
A
338
48.053
−1.802
−6.268
1.00
12.45
A
C


ATOM
2771
O
THR
A
338
48.577
−2.461
−7.149
1.00
13.56
A
O


ATOM
2772
N
ILE
A
339
46.785
−1.961
−5.929
1.00
13.24
A
N


ATOM
2773
CA
ILE
A
339
45.953
−2.999
−6.549
1.00
14.77
A
C


ATOM
2774
CB
ILE
A
339
44.613
−3.228
−5.817
1.00
15.84
A
C


ATOM
2775
CG1
ILE
A
339
44.805
−3.733
−4.366
1.00
22.56
A
C


ATOM
2776
CD1
ILE
A
339
46.091
−4.409
−4.060
1.00
25.24
A
C


ATOM
2777
CG2
ILE
A
339
43.713
−4.206
−6.587
1.00
16.00
A
C


ATOM
2778
C
ILE
A
339
45.683
−2.616
−8.021
1.00
16.22
A
C


ATOM
2779
O
ILE
A
339
45.803
−3.467
−8.907
1.00
15.73
A
O


ATOM
2780
N
ASN
A
340
45.338
−1.354
−8.272
1.00
13.61
A
N


ATOM
2781
CA
ASN
A
340
45.132
−0.858
−9.633
1.00
13.64
A
C


ATOM
2782
CB
ASN
A
340
44.643
0.610
−9.648
1.00
14.73
A
C


ATOM
2783
CG
ASN
A
340
43.212
0.767
−9.124
1.00
14.26
A
C


ATOM
2784
OD1
ASN
A
340
42.491
−0.210
−8.962
1.00
14.11
A
O


ATOM
2785
ND2
ASN
A
340
42.814
1.994
−8.837
1.00
12.66
A
N


ATOM
2786
C
ASN
A
340
46.366
−1.081
−10.536
1.00
15.34
A
C


ATOM
2787
O
ASN
A
340
46.246
−1.359
−11.737
1.00
15.44
A
O


ATOM
2788
N
GLU
A
341
47.554
−0.973
−9.961
1.00
14.07
A
N


ATOM
2789
CA
AGLU
A
341
48.806
−1.157
−10.703
0.50
13.47
A
C


ATOM
2790
CA
BGLU
A
341
48.772
−1.131
−10.741
0.50
13.96
A
C


ATOM
2791
CB
AGLU
A
341
49.993
−0.618
−9.887
0.50
16.02
A
C


ATOM
2792
CB
BGLU
A
341
49.949
−0.382
−10.060
0.50
17.37
A
C


ATOM
2793
CG
AGLU
A
341
51.368
−0.951
−10.447
0.50
17.69
A
C


ATOM
2794
CG
BGLU
A
341
49.804
1.161
−10.206
0.50
22.08
A
C


ATOM
2795
CD
AGLU
A
341
52.459
−0.118
−9.803
0.50
20.91
A
C


ATOM
2796
CD
BGLU
A
341
50.629
2.019
−9.226
0.50
27.60
A
C


ATOM
2797
OE1
BGLU
A
341
51.572
1.483
−8.603
0.50
23.51
A
O


ATOM
2798
OE1
AGLU
A
341
53.226
0.497
−10.561
0.50
25.96
A
O


ATOM
2799
OE2
AGLU
A
341
52.521
−0.056
−8.544
0.50
24.48
A
O


ATOM
2800
OE2
BGLU
A
341
50.328
3.245
−9.094
0.50
24.19
A
O


ATOM
2801
C
GLU
A
341
49.026
−2.631
−11.054
1.00
12.91
A
C


ATOM
2802
O
GLU
A
341
49.462
−2.961
−12.159
1.00
12.81
A
O


ATOM
2803
N
ILE
A
342
48.697
−3.516
−10.127
1.00
13.30
A
N


ATOM
2804
CA
ILE
A
342
48.748
−4.965
−10.378
1.00
15.54
A
C


ATOM
2805
CB
ILE
A
342
48.523
−5.766
−9.104
1.00
13.99
A
C


ATOM
2806
CG1
ILE
A
342
49.746
−5.616
−8.187
1.00
14.53
A
C


ATOM
2807
CD1
ILE
A
342
49.481
−6.036
−6.734
1.00
14.11
A
C


ATOM
2808
CG2
ILE
A
342
48.296
−7.246
−9.408
1.00
16.66
A
C


ATOM
2809
C
ILE
A
342
47.733
−5.337
−11.469
1.00
18.15
A
C


ATOM
2810
O
ILE
A
342
48.076
−6.076
−12.401
1.00
17.80
A
O


ATOM
2811
N
ALA
A
343
46.533
−4.762
−11.400
1.00
16.13
A
N


ATOM
2812
CA
ALA
A
343
45.528
−4.937
−12.481
1.00
14.03
A
C


ATOM
2813
CB
ALA
A
343
44.234
−4.211
−12.169
1.00
11.91
A
C


ATOM
2814
C
ALA
A
343
46.092
−4.480
−13.786
1.00
14.80
A
C


ATOM
2815
O
ALA
A
343
45.925
−5.172
−14.784
1.00
14.81
A
O


ATOM
2816
N
TYR
A
344
46.755
−3.312
−13.810
1.00
12.84
A
N


ATOM
2817
CA
TYR
A
344
47.363
−2.802
−15.039
1.00
13.86
A
C


ATOM
2818
CB
TYR
A
344
48.045
−1.423
−14.850
1.00
11.49
A
C


ATOM
2819
CG
TYR
A
344
48.692
−0.929
−16.100
1.00
9.85
A
C


ATOM
2820
CD1
TYR
A
344
47.939
−0.286
−17.074
1.00
9.93
A
C


ATOM
2821
CE1
TYR
A
344
48.493
0.161
−18.240
1.00
8.79
A
C


ATOM
2822
CZ
TYR
A
344
49.824
−0.027
−18.469
1.00
9.72
A
C


ATOM
2823
OH
TYR
A
344
50.296
0.416
−19.655
1.00
11.12
A
O


ATOM
2824
CE2
TYR
A
344
50.618
−0.658
−17.532
1.00
10.17
A
C


ATOM
2825
CD2
TYR
A
344
50.042
−1.071
−16.324
1.00
9.90
A
C


ATOM
2826
C
TYR
A
344
48.377
−3.790
−15.620
1.00
14.30
A
C


ATOM
2827
O
TYR
A
344
48.350
−4.081
−16.799
1.00
11.84
A
O


ATOM
2828
N
ASP
A
345
49.286
−4.277
−14.791
1.00
15.91
A
N


ATOM
2829
CA
ASP
A
345
50.307
−5.205
−15.280
1.00
17.61
A
C


ATOM
2830
CB
ASP
A
345
51.222
−5.703
−14.157
1.00
22.91
A
C


ATOM
2831
CG
ASP
A
345
52.054
−4.585
−13.478
1.00
24.91
A
C


ATOM
2832
OD1
ASP
A
345
52.328
−3.540
−14.082
1.00
29.76
A
O


ATOM
2833
OD2
ASP
A
345
52.410
−4.789
−12.296
1.00
40.11
A
O


ATOM
2834
C
ASP
A
345
49.638
−6.444
−15.929
1.00
17.32
A
C


ATOM
2835
O
ASP
A
345
50.124
−6.957
−16.929
1.00
16.55
A
O


ATOM
2836
N
ASN
A
346
48.552
−6.925
−15.331
1.00
16.63
A
N


ATOM
2837
CA
ASN
A
346
47.836
−8.075
−15.869
1.00
15.25
A
C


ATOM
2838
CB
ASN
A
346
46.897
−8.661
−14.839
1.00
18.38
A
C


ATOM
2839
CG
ASN
A
346
47.595
−9.570
−13.917
1.00
25.49
A
C


ATOM
2840
OD1
ASN
A
346
47.711
−10.781
−14.186
1.00
19.59
A
O


ATOM
2841
ND2
ASN
A
346
48.093
−9.015
−12.810
1.00
23.37
A
N


ATOM
2842
C
ASN
A
346
47.078
−7.787
−17.158
1.00
16.76
A
C


ATOM
2843
O
ASN
A
346
47.093
−8.610
−18.062
1.00
19.88
A
O


ATOM
2844
N
LEU
A
347
46.490
−6.597
−17.266
1.00
15.52
A
N


ATOM
2845
CA
LEU
A
347
45.902
−6.120
−18.514
1.00
13.62
A
C


ATOM
2846
CB
LEU
A
347
45.198
−4.779
−18.323
1.00
11.95
A
C


ATOM
2847
CG
LEU
A
347
44.492
−4.169
−19.533
1.00
11.99
A
C


ATOM
2848
CD1
LEU
A
347
43.413
−5.129
−20.038
1.00
13.90
A
C


ATOM
2849
CD2
LEU
A
347
43.932
−2.771
−19.199
1.00
13.13
A
C


ATOM
2850
C
LEU
A
347
46.940
−6.078
−19.629
1.00
17.97
A
C


ATOM
2851
O
LEU
A
347
46.736
−6.649
−20.730
1.00
16.03
A
O


ATOM
2852
N
LYS
A
348
48.081
−5.472
−19.332
1.00
13.81
A
N


ATOM
2853
CA
LYS
A
348
49.117
−5.318
−20.306
1.00
14.92
A
C


ATOM
2854
CB
LYS
A
348
50.236
−4.463
−19.738
1.00
18.92
A
C


ATOM
2855
CG
LYS
A
348
51.303
−4.159
−20.734
1.00
22.39
A
C


ATOM
2856
CD
LYS
A
348
52.244
−3.064
−20.262
1.00
25.50
A
C


ATOM
2857
CE
LYS
A
348
53.608
−3.563
−19.902
1.00
30.70
A
C


ATOM
2858
NZ
LYS
A
348
53.587
−4.143
−18.593
1.00
33.98
A
N


ATOM
2859
C
LYS
A
348
49.713
−6.659
−20.779
1.00
15.93
A
C


ATOM
2860
O
LYS
A
348
49.978
−6.835
−21.970
1.00
15.69
A
O


ATOM
2861
N
ASP
A
349
49.985
−7.533
−19.830
1.00
15.10
A
N


ATOM
2862
CA
ASP
A
349
50.727
−8.774
−20.103
1.00
22.90
A
C


ATOM
2863
CB
ASP
A
349
51.600
−9.157
−18.888
1.00
18.72
A
C


ATOM
2864
CG
ASP
A
349
52.706
−8.090
−18.606
1.00
28.43
A
C


ATOM
2865
OD1
ASP
A
349
53.075
−7.326
−19.532
1.00
31.35
A
O


ATOM
2866
OD2
ASP
A
349
53.179
−7.993
−17.459
1.00
49.50
A
O


ATOM
2867
C
ASP
A
349
49.826
−9.928
−20.532
1.00
22.68
A
C


ATOM
2868
O
ASP
A
349
50.226
−10.709
−21.348
1.00
22.80
A
O


ATOM
2869
N
LYS
A
350
48.616
−10.020
−19.982
1.00
21.26
A
N


ATOM
2870
CA
LYS
A
350
47.716
−11.160
−20.247
1.00
21.09
A
C


ATOM
2871
CB
LYS
A
350
47.238
−11.768
−18.935
1.00
25.36
A
C


ATOM
2872
CG
LYS
A
350
48.324
−12.371
−18.044
1.00
25.43
A
C


ATOM
2873
CD
LYS
A
350
47.703
−12.850
−16.709
1.00
44.76
A
C


ATOM
2874
CE
LYS
A
350
48.773
−13.100
−15.605
1.00
41.38
A
C


ATOM
2875
NZ
LYS
A
350
49.841
−13.998
−16.126
1.00
67.10
A
N


ATOM
2876
C
LYS
A
350
46.481
−10.754
−21.045
1.00
25.46
A
C


ATOM
2877
O
LYS
A
350
45.727
−11.597
−21.473
1.00
22.82
A
O


ATOM
2878
N
GLY
A
351
46.217
−9.458
−21.184
1.00
17.25
A
N


ATOM
2879
CA
GLY
A
351
45.118
−9.004
−22.002
1.00
19.61
A
C


ATOM
2880
C
GLY
A
351
43.774
−9.129
−21.322
1.00
19.39
A
C


ATOM
2881
O
GLY
A
351
42.753
−8.986
−21.972
1.00
20.33
A
O


ATOM
2882
N
GLU
A
352
43.764
−9.287
−20.006
1.00
17.50
A
N


ATOM
2883
CA
GLU
A
352
42.529
−9.442
−19.253
1.00
20.07
A
C


ATOM
2884
CB
GLU
A
352
42.542
−10.806
−18.546
1.00
23.74
A
C


ATOM
2885
CG
GLU
A
352
42.695
−12.044
−19.444
1.00
36.13
A
C


ATOM
2886
CD
GLU
A
352
41.454
−12.383
−20.264
1.00
42.77
A
C


ATOM
2887
OE1
GLU
A
352
40.402
−11.710
−20.115
1.00
44.84
A
O


ATOM
2888
OE2
GLU
A
352
41.551
−13.335
−21.074
1.00
62.66
A
O


ATOM
2889
C
GLU
A
352
42.423
−8.383
−18.166
1.00
19.66
A
C


ATOM
2890
O
GLU
A
352
43.444
−8.030
−17.542
1.00
15.51
A
O


ATOM
2891
N
ASN
A
353
41.203
−7.934
−17.898
1.00
20.74
A
N


ATOM
2892
CA
ASN
A
353
40.918
−7.090
−16.739
1.00
22.43
A
C


ATOM
2893
CB
ASN
A
353
39.822
−6.099
−17.083
1.00
23.62
A
C


ATOM
2894
CG
ASN
A
353
39.486
−5.180
−15.917
1.00
19.76
A
C


ATOM
2895
OD1
ASN
A
353
40.047
−5.295
−14.821
1.00
20.21
A
O


ATOM
2896
ND2
ASN
A
353
38.565
−4.287
−16.141
1.00
23.44
A
N


ATOM
2897
C
ASN
A
353
40.535
−7.929
−15.510
1.00
23.59
A
C


ATOM
2898
O
ASN
A
353
39.426
−8.430
−15.432
1.00
23.24
A
O


ATOM
2899
N
ILE
A
354
41.448
−8.068
−14.551
1.00
16.39
A
N


ATOM
2900
CA
ILE
A
354
41.213
−8.909
−13.377
1.00
18.18
A
C


ATOM
2901
CB
ILE
A
354
42.471
−9.826
−13.071
1.00
20.87
A
C


ATOM
2902
CG1
ILE
A
354
43.685
−8.961
−12.679
1.00
23.86
A
C


ATOM
2903
CD1
ILE
A
354
44.869
−9.681
−12.114
1.00
22.04
A
C


ATOM
2904
CG2
ILE
A
354
42.818
−10.649
−14.246
1.00
26.49
A
C


ATOM
2905
C
ILE
A
354
40.863
−8.076
−12.134
1.00
17.05
A
C


ATOM
2906
O
ILE
A
354
40.740
−8.614
−11.023
1.00
16.63
A
O


ATOM
2907
N
LEU
A
355
40.698
−6.776
−12.313
1.00
15.40
A
N


ATOM
2908
CA
LEU
A
355
40.503
−5.870
−11.179
1.00
16.59
A
C


ATOM
2909
CB
LEU
A
355
40.312
−4.422
−11.658
1.00
15.17
A
C


ATOM
2910
CG
LEU
A
355
40.244
−3.371
−10.554
1.00
16.92
A
C


ATOM
2911
CD1
LEU
A
355
41.444
−3.440
−9.684
1.00
12.65
A
C


ATOM
2912
CD2
LEU
A
355
40.076
−1.969
−11.169
1.00
15.94
A
C


ATOM
2913
C
LEU
A
355
39.307
−6.277
−10.343
1.00
19.71
A
C


ATOM
2914
O
LEU
A
355
39.411
−6.270
−9.125
1.00
20.01
A
O


ATOM
2915
N
PRO
A
356
38.197
−6.733
−10.994
1.00
19.04
A
N


ATOM
2916
CA
PRO
A
356
37.082
−7.168
−10.157
1.00
22.46
A
C


ATOM
2917
CB
PRO
A
356
36.016
−7.617
−11.181
1.00
27.85
A
C


ATOM
2918
CG
PRO
A
356
36.381
−6.909
−12.466
1.00
26.81
A
C


ATOM
2919
CD
PRO
A
356
37.871
−6.785
−12.440
1.00
18.70
A
C


ATOM
2920
C
PRO
A
356
37.425
−8.284
−9.165
1.00
17.63
A
C


ATOM
2921
O
PRO
A
356
36.912
−8.276
−8.056
1.00
19.64
A
O


ATOM
2922
N
TYR
A
357
38.292
−9.222
−9.535
1.00
17.75
A
N


ATOM
2923
CA
TYR
A
357
38.639
−10.313
−8.666
1.00
18.75
A
C


ATOM
2924
CB
TYR
A
357
39.296
−11.463
−9.439
1.00
23.81
A
C


ATOM
2925
CG
TYR
A
357
38.510
−11.780
−10.695
1.00
27.29
A
C


ATOM
2926
CD1
TYR
A
357
37.253
−12.358
−10.623
1.00
43.84
A
C


ATOM
2927
CE1
TYR
A
357
36.495
−12.599
−11.777
1.00
48.29
A
C


ATOM
2928
CZ
TYR
A
357
36.998
−12.243
−13.007
1.00
43.38
A
C


ATOM
2929
OH
TYR
A
357
36.278
−12.488
−14.147
1.00
57.79
A
O


ATOM
2930
CE2
TYR
A
357
38.240
−11.670
−13.109
1.00
35.02
A
C


ATOM
2931
CD2
TYR
A
357
38.992
−11.438
−11.941
1.00
30.70
A
C


ATOM
2932
C
TYR
A
357
39.569
−9.861
−7.539
1.00
20.46
A
C


ATOM
2933
O
TYR
A
357
39.448
−10.336
−6.424
1.00
17.34
A
O


ATOM
2934
N
LEU
A
358
40.505
−8.963
−7.837
1.00
19.62
A
N


ATOM
2935
CA
LEU
A
358
41.416
−8.435
−6.816
1.00
18.46
A
C


ATOM
2936
CB
LEU
A
358
42.504
−7.567
−7.462
1.00
17.42
A
C


ATOM
2937
CG
LEU
A
358
43.342
−8.234
−8.557
1.00
18.52
A
C


ATOM
2938
CD1
LEU
A
358
44.447
−7.314
−9.129
1.00
13.44
A
C


ATOM
2939
CD2
LEU
A
358
43.965
−9.585
−8.082
1.00
15.36
A
C


ATOM
2940
C
LEU
A
358
40.643
−7.607
−5.802
1.00
18.16
A
C


ATOM
2941
O
LEU
A
358
40.897
−7.703
−4.621
1.00
18.07
A
O


ATOM
2942
N
THR
A
359
39.706
−6.769
−6.258
1.00
17.16
A
N


ATOM
2943
CA
THR
A
359
38.970
−5.910
−5.340
1.00
19.21
A
C


ATOM
2944
CB
THR
A
359
38.265
−4.733
−6.053
1.00
19.64
A
C


ATOM
2945
OG1
THR
A
359
37.306
−5.211
−7.012
1.00
17.02
A
O


ATOM
2946
CG2
THR
A
359
39.292
−3.852
−6.759
1.00
17.09
A
C


ATOM
2947
C
THR
A
359
37.986
−6.703
−4.461
1.00
20.32
A
C


ATOM
2948
O
THR
A
359
37.809
−6.396
−3.291
1.00
17.74
A
O


ATOM
2949
N
LYS
A
360
37.426
−7.774
−5.000
1.00
21.30
A
N


ATOM
2950
CA
LYS
A
360
36.511
−8.616
−4.227
1.00
20.03
A
C


ATOM
2951
CB
LYS
A
360
35.804
−9.623
−5.139
1.00
21.21
A
C


ATOM
2952
CG
LYS
A
360
34.793
−10.541
−4.420
1.00
24.65
A
C


ATOM
2953
CD
LYS
A
360
33.613
−9.722
−3.911
1.00
35.48
A
C


ATOM
2954
CE
LYS
A
360
32.377
−10.588
−3.593
1.00
39.73
A
C


ATOM
2955
NZ
LYS
A
360
32.596
−11.358
−2.350
1.00
34.91
A
N


ATOM
2956
C
LYS
A
360
37.269
−9.304
−3.108
1.00
18.87
A
C


ATOM
2957
O
LYS
A
360
36.755
−9.380
−2.000
1.00
16.32
A
O


ATOM
2958
N
ALA
A
361
38.484
−9.787
−3.390
1.00
17.75
A
N


ATOM
2959
CA
ALA
A
361
39.342
−10.403
−2.377
1.00
17.01
A
C


ATOM
2960
CB
ALA
A
361
40.691
−10.895
−2.975
1.00
16.89
A
C


ATOM
2961
C
ALA
A
361
39.581
−9.431
−1.254
1.00
21.37
A
C


ATOM
2962
O
ALA
A
361
39.459
−9.793
−0.086
1.00
18.78
A
O


ATOM
2963
N
TRP
A
362
39.869
−8.168
−1.589
1.00
16.02
A
N


ATOM
2964
CA
TRP
A
362
40.057
−7.156
−0.530
1.00
16.57
A
C


ATOM
2965
CB
TRP
A
362
40.683
−5.842
−1.117
1.00
15.44
A
C


ATOM
2966
CG
TRP
A
362
42.175
−6.013
−1.080
1.00
15.77
A
C


ATOM
2967
CD1
TRP
A
362
43.022
−6.410
−2.106
1.00
15.92
A
C


ATOM
2968
NE1
TRP
A
362
44.291
−6.530
−1.649
1.00
16.94
A
N


ATOM
2969
CE2
TRP
A
362
44.346
−6.288
−0.320
1.00
17.44
A
C


ATOM
2970
CD2
TRP
A
362
43.006
−5.980
0.113
1.00
14.82
A
C


ATOM
2971
CE3
TRP
A
362
42.779
−5.658
1.442
1.00
14.35
A
C


ATOM
2972
CZ3
TRP
A
362
43.853
−5.674
2.323
1.00
16.82
A
C


ATOM
2973
CH2
TRP
A
362
45.132
−5.996
1.892
1.00
18.89
A
C


ATOM
2974
CZ2
TRP
A
362
45.406
−6.316
0.563
1.00
17.80
A
C


ATOM
2975
C
TRP
A
362
38.778
−6.870
0.258
1.00
16.02
A
C


ATOM
2976
O
TRP
A
362
38.825
−6.702
1.479
1.00
17.20
A
O


ATOM
2977
N
ALA
A
363
37.635
−6.790
−0.425
1.00
15.47
A
N


ATOM
2978
CA
ALA
A
363
36.345
−6.545
0.264
1.00
15.85
A
C


ATOM
2979
CB
ALA
A
363
35.192
−6.378
−0.737
1.00
13.63
A
C


ATOM
2980
C
ALA
A
363
36.043
−7.692
1.209
1.00
17.54
A
C


ATOM
2981
O
ALA
A
363
35.605
−7.482
2.357
1.00
15.67
A
O


ATOM
2982
N
ASP
A
364
36.266
−8.912
0.730
1.00
17.17
A
N


ATOM
2983
CA
ASP
A
364
36.021
−10.081
1.558
1.00
19.58
A
C


ATOM
2984
CB
ASP
A
364
36.261
−11.379
0.777
1.00
18.80
A
C


ATOM
2985
CG
ASP
A
364
35.186
−11.654
−0.277
1.00
25.03
A
C


ATOM
2986
OD1
ASP
A
364
34.163
−10.933
−0.313
1.00
24.08
A
O


ATOM
2987
OD2
ASP
A
364
35.411
−12.586
−1.089
1.00
30.13
A
O


ATOM
2988
C
ASP
A
364
36.923
−10.077
2.775
1.00
21.21
A
C


ATOM
2989
O
ASP
A
364
36.488
−10.396
3.879
1.00
22.54
A
O


ATOM
2990
N
LEU
A
365
38.180
−9.700
2.602
1.00
17.93
A
N


ATOM
2991
CA
LEU
A
365
39.091
−9.657
3.759
1.00
15.56
A
C


ATOM
2992
CB
LEU
A
365
40.539
−9.491
3.316
1.00
18.51
A
C


ATOM
2993
CG
LEU
A
365
41.573
−9.334
4.445
1.00
19.97
A
C


ATOM
2994
CD1
LEU
A
365
41.577
−10.605
5.310
1.00
18.02
A
C


ATOM
2995
CD2
LEU
A
365
42.952
−9.086
3.812
1.00
18.89
A
C


ATOM
2996
C
LEU
A
365
38.705
−8.584
4.751
1.00
15.45
A
C


ATOM
2997
O
LEU
A
365
38.648
−8.831
5.949
1.00
16.28
A
O


ATOM
2998
N
CYS
A
366
38.450
−7.376
4.253
1.00
16.26
A
N


ATOM
2999
CA
CYS
A
366
37.990
−6.297
5.112
1.00
17.29
A
C


ATOM
3000
CB
CYS
A
366
37.850
−4.994
4.322
1.00
17.89
A
C


ATOM
3001
SG
CYS
A
366
39.503
−4.353
3.920
1.00
21.78
A
S


ATOM
3002
C
CYS
A
366
36.698
−6.658
5.838
1.00
17.80
A
C


ATOM
3003
O
CYS
A
366
36.572
−6.362
7.025
1.00
16.43
A
O


ATOM
3004
N
ASN
A
367
35.776
−7.340
5.157
1.00
18.27
A
N


ATOM
3005
CA
ASN
A
367
34.563
−7.799
5.849
1.00
19.89
A
C


ATOM
3006
CB
ASN
A
367
33.480
−8.314
4.879
1.00
19.83
A
C


ATOM
3007
CG
ASN
A
367
32.559
−7.191
4.413
1.00
22.39
A
C


ATOM
3008
OD1
ASN
A
367
31.795
−6.645
5.211
1.00
25.95
A
O


ATOM
3009
ND2
ASN
A
367
32.654
−6.820
3.141
1.00
21.60
A
N


ATOM
3010
C
ASN
A
367
34.838
−8.831
6.930
1.00
17.90
A
C


ATOM
3011
O
ASN
A
367
34.139
−8.845
7.947
1.00
19.27
A
O


ATOM
3012
N
ALA
A
368
35.835
−9.690
6.724
1.00
17.50
A
N


ATOM
3013
CA
ALA
A
368
36.199
−10.694
7.735
1.00
16.98
A
C


ATOM
3014
CB
ALA
A
368
37.171
−11.765
7.159
1.00
15.68
A
C


ATOM
3015
C
ALA
A
368
36.829
−9.969
8.953
1.00
17.79
A
C


ATOM
3016
O
ALA
A
368
36.525
−10.318
10.104
1.00
15.50
A
O


ATOM
3017
N
PHE
A
369
37.692
−8.966
8.714
1.00
17.82
A
N


ATOM
3018
CA
PHE
A
369
38.229
−8.147
9.824
1.00
16.77
A
C


ATOM
3019
CB
PHE
A
369
39.221
−7.071
9.339
1.00
18.30
A
C


ATOM
3020
CG
PHE
A
369
40.568
−7.590
8.837
1.00
19.24
A
C


ATOM
3021
CD1
PHE
A
369
41.131
−8.763
9.306
1.00
19.40
A
C


ATOM
3022
CE1
PHE
A
369
42.396
−9.193
8.869
1.00
26.18
A
C


ATOM
3023
CZ
PHE
A
369
43.111
−8.439
7.950
1.00
23.12
A
C


ATOM
3024
CE2
PHE
A
369
42.564
−7.263
7.477
1.00
22.91
A
C


ATOM
3025
CD2
PHE
A
369
41.304
−6.828
7.940
1.00
22.68
A
C


ATOM
3026
C
PHE
A
369
37.092
−7.420
10.562
1.00
16.24
A
C


ATOM
3027
O
PHE
A
369
37.101
−7.327
11.798
1.00
16.27
A
O


ATOM
3028
N
LEU
A
370
36.156
−6.842
9.815
1.00
16.48
A
N


ATOM
3029
CA
LEU
A
370
35.027
−6.121
10.447
1.00
16.80
A
C


ATOM
3030
CB
LEU
A
370
34.116
−5.493
9.387
1.00
17.12
A
C


ATOM
3031
CG
LEU
A
370
32.976
−4.592
9.855
1.00
18.29
A
C


ATOM
3032
CD1
LEU
A
370
33.490
−3.370
10.629
1.00
15.76
A
C


ATOM
3033
CD2
LEU
A
370
32.150
−4.145
8.629
1.00
18.79
A
C


ATOM
3034
C
LEU
A
370
34.220
−7.045
11.361
1.00
15.54
A
C


ATOM
3035
O
LEU
A
370
33.890
−6.677
12.490
1.00
14.64
A
O


ATOM
3036
N
GLN
A
371
33.947
−8.263
10.896
1.00
16.12
A
N


ATOM
3037
CA
GLN
A
371
33.151
−9.219
11.699
1.00
14.85
A
C


ATOM
3038
CB
GLN
A
371
32.855
−10.487
10.893
1.00
17.23
A
C


ATOM
3039
CG
GLN
A
371
32.175
−11.586
11.707
1.00
20.63
A
C


ATOM
3040
CD
GLN
A
371
30.784
−11.161
12.089
1.00
22.06
A
C


ATOM
3041
OE1
GLN
A
371
30.550
−10.601
13.173
1.00
21.46
A
O


ATOM
3042
NE2
GLN
A
371
29.874
−11.320
11.162
1.00
21.27
A
N


ATOM
3043
C
GLN
A
371
33.886
−9.539
12.994
1.00
14.40
A
C


ATOM
3044
O
GLN
A
371
33.276
−9.607
14.067
1.00
14.54
A
O


ATOM
3045
N
GLU
A
372
35.212
−9.653
12.932
1.00
16.62
A
N


ATOM
3046
CA
GLU
A
372
36.005
−9.834
14.162
1.00
19.01
A
C


ATOM
3047
CB
GLU
A
372
37.491
−10.119
13.857
1.00
20.98
A
C


ATOM
3048
CG
GLU
A
372
37.711
−11.554
13.396
1.00
29.08
A
C


ATOM
3049
CD
GLU
A
372
39.158
−11.908
12.942
1.00
33.39
A
C


ATOM
3050
OE1
GLU
A
372
40.011
−10.990
12.711
1.00
34.79
A
O


ATOM
3051
OE2
GLU
A
372
39.407
−13.143
12.811
1.00
29.74
A
O


ATOM
3052
C
GLU
A
372
35.918
−8.648
15.108
1.00
17.90
A
C


ATOM
3053
O
GLU
A
372
35.813
−8.810
16.310
1.00
15.75
A
O


ATOM
3054
N
ALA
A
373
36.024
−7.455
14.552
1.00
17.76
A
N


ATOM
3055
CA
ALA
A
373
35.941
−6.235
15.352
1.00
19.33
A
C


ATOM
3056
CB
ALA
A
373
36.161
−5.006
14.449
1.00
17.45
A
C


ATOM
3057
C
ALA
A
373
34.559
−6.177
16.042
1.00
18.40
A
C


ATOM
3058
O
ALA
A
373
34.439
−5.813
17.217
1.00
18.25
A
O


ATOM
3059
N
LYS
A
374
33.523
−6.533
15.304
1.00
18.27
A
N


ATOM
3060
CA
LYS
A
374
32.148
−6.485
15.839
1.00
23.07
A
C


ATOM
3061
CB
LYS
A
374
31.127
−6.708
14.722
1.00
25.62
A
C


ATOM
3062
CG
LYS
A
374
30.946
−5.491
13.852
1.00
30.72
A
C


ATOM
3063
CD
LYS
A
374
29.908
−5.715
12.751
1.00
33.77
A
C


ATOM
3064
CE
LYS
A
374
29.633
−4.423
11.995
1.00
32.01
A
C


ATOM
3065
NZ
LYS
A
374
28.908
−4.688
10.703
1.00
28.44
A
N


ATOM
3066
C
LYS
A
374
31.922
−7.489
16.970
1.00
21.53
A
C


ATOM
3067
O
LYS
A
374
31.345
−7.148
17.994
1.00
22.96
A
O


ATOM
3068
N
TRP
A
375
32.343
−8.731
16.773
1.00
21.14
A
N


ATOM
3069
CA
TRP
A
375
32.234
−9.734
17.824
1.00
20.44
A
C


ATOM
3070
CB
TRP
A
375
32.831
−11.056
17.375
1.00
17.79
A
C


ATOM
3071
CG
TRP
A
375
31.969
−11.869
16.458
1.00
17.05
A
C


ATOM
3072
CD1
TRP
A
375
30.592
−11.922
16.408
1.00
19.16
A
C


ATOM
3073
NE1
TRP
A
375
30.175
−12.815
15.454
1.00
20.51
A
N


ATOM
3074
CE2
TRP
A
375
31.207
−13.387
14.862
1.00
19.06
A
C


ATOM
3075
CD2
TRP
A
375
32.413
−12.826
15.465
1.00
16.94
A
C


ATOM
3076
CE3
TRP
A
375
33.651
−13.251
15.017
1.00
18.68
A
C


ATOM
3077
CZ3
TRP
A
375
33.692
−14.221
14.022
1.00
18.14
A
C


ATOM
3078
CH2
TRP
A
375
32.516
−14.753
13.457
1.00
20.50
A
C


ATOM
3079
CZ2
TRP
A
375
31.256
−14.357
13.863
1.00
18.39
A
C


ATOM
3080
C
TRP
A
375
32.934
−9.300
19.096
1.00
24.17
A
C


ATOM
3081
O
TRP
A
375
32.446
−9.538
20.197
1.00
23.79
A
O


ATOM
3082
N
LEU
A
376
34.094
−8.684
18.954
1.00
21.61
A
N


ATOM
3083
CA
LEU
A
376
34.879
−8.243
20.111
1.00
25.50
A
C


ATOM
3084
CB
LEU
A
376
36.252
−7.756
19.649
1.00
29.13
A
C


ATOM
3085
CG
LEU
A
376
37.352
−7.786
20.704
1.00
40.86
A
C


ATOM
3086
CD1
LEU
A
376
37.818
−9.236
20.886
1.00
47.30
A
C


ATOM
3087
CD2
LEU
A
376
38.521
−6.872
20.313
1.00
40.17
A
C


ATOM
3088
C
LEU
A
376
34.173
−7.108
20.853
1.00
24.00
A
C


ATOM
3089
O
LEU
A
376
34.076
−7.113
22.072
1.00
24.75
A
O


ATOM
3090
N
TYR
A
377
33.682
−6.135
20.094
1.00
21.41
A
N


ATOM
3091
CA
TYR
A
377
33.004
−4.999
20.663
1.00
22.87
A
C


ATOM
3092
CB
TYR
A
377
32.630
−4.002
19.556
1.00
25.18
A
C


ATOM
3093
CG
TYR
A
377
31.858
−2.792
20.054
1.00
27.72
A
C


ATOM
3094
CD1
TYR
A
377
32.491
−1.794
20.806
1.00
34.13
A
C


ATOM
3095
CE1
TYR
A
377
31.777
−0.673
21.288
1.00
36.47
A
C


ATOM
3096
CZ
TYR
A
377
30.425
−0.549
21.001
1.00
38.76
A
C


ATOM
3097
OH
TYR
A
377
29.719
0.539
21.465
1.00
46.84
A
O


ATOM
3098
CE2
TYR
A
377
29.773
−1.526
20.248
1.00
38.56
A
C


ATOM
3099
CD2
TYR
A
377
30.499
−2.641
19.776
1.00
33.27
A
C


ATOM
3100
C
TYR
A
377
31.742
−5.446
21.425
1.00
26.24
A
C


ATOM
3101
O
TYR
A
377
31.482
−4.971
22.514
1.00
26.71
A
O


ATOM
3102
N
ASN
A
378
30.958
−6.344
20.829
1.00
24.22
A
N


ATOM
3103
CA
ASN
A
378
29.704
−6.794
21.429
1.00
27.32
A
C


ATOM
3104
CB
ASN
A
378
28.724
−7.198
20.341
1.00
28.78
A
C


ATOM
3105
CG
ASN
A
378
28.337
−6.036
19.460
1.00
28.02
A
C


ATOM
3106
OD1
ASN
A
378
28.096
−4.938
19.937
1.00
36.83
A
O


ATOM
3107
ND2
ASN
A
378
28.263
−6.275
18.176
1.00
34.11
A
N


ATOM
3108
C
ASN
A
378
29.896
−7.947
22.393
1.00
26.44
A
C


ATOM
3109
O
ASN
A
378
28.933
−8.441
22.949
1.00
22.29
A
O


ATOM
3110
N
LYS
A
379
31.144
−8.371
22.584
1.00
27.62
A
N


ATOM
3111
CA
LYS
A
379
31.454
−9.531
23.411
1.00
28.83
A
C


ATOM
3112
CB
LYS
A
379
31.256
−9.169
24.895
1.00
31.31
A
C


ATOM
3113
CG
LYS
A
379
32.247
−8.093
25.353
1.00
38.56
A
C


ATOM
3114
CD
LYS
A
379
31.787
−7.303
26.564
1.00
45.70
A
C


ATOM
3115
CE
LYS
A
379
32.906
−6.401
27.055
1.00
47.62
A
C


ATOM
3116
NZ
LYS
A
379
32.469
−5.534
28.178
1.00
57.66
A
N


ATOM
3117
C
LYS
A
379
30.638
−10.765
22.980
1.00
25.15
A
C


ATOM
3118
O
LYS
A
379
30.102
−11.492
23.807
1.00
28.12
A
O


ATOM
3119
N
SER
A
380
30.545
−10.992
21.675
1.00
24.80
A
N


ATOM
3120
CA
SER
A
380
29.787
−12.121
21.144
1.00
22.46
A
C


ATOM
3121
CB
SER
A
380
29.565
−11.957
19.645
1.00
24.96
A
C


ATOM
3122
OG
SER
A
380
29.059
−10.652
19.336
1.00
20.52
A
O


ATOM
3123
C
SER
A
380
30.510
−13.425
21.458
1.00
22.51
A
C


ATOM
3124
O
SER
A
380
31.691
−13.437
21.764
1.00
27.18
A
O


ATOM
3125
N
THR
A
381
29.773
−14.520
21.429
1.00
22.40
A
N


ATOM
3126
CA
THR
A
381
30.302
−15.827
21.715
1.00
22.08
A
C


ATOM
3127
CB
THR
A
381
29.715
−16.422
23.058
1.00
24.95
A
C


ATOM
3128
OG1
THR
A
381
28.288
−16.450
22.963
1.00
26.79
A
O


ATOM
3129
CG2
THR
A
381
30.081
−15.588
24.317
1.00
21.92
A
C


ATOM
3130
C
THR
A
381
29.868
−16.700
20.537
1.00
23.52
A
C


ATOM
3131
O
THR
A
381
29.037
−17.575
20.699
1.00
23.64
A
O


ATOM
3132
N
PRO
A
382
30.413
−16.462
19.333
1.00
22.31
A
N


ATOM
3133
CA
PRO
A
382
30.015
−17.325
18.219
1.00
18.54
A
C


ATOM
3134
CB
PRO
A
382
30.707
−16.680
17.017
1.00
22.53
A
C


ATOM
3135
CG
PRO
A
382
31.991
−16.111
17.629
1.00
22.28
A
C


ATOM
3136
CD
PRO
A
382
31.444
−15.491
18.934
1.00
22.64
A
C


ATOM
3137
C
PRO
A
382
30.536
−18.749
18.416
1.00
21.29
A
C


ATOM
3138
O
PRO
A
382
31.526
−18.964
19.124
1.00
24.96
A
O


ATOM
3139
N
THR
A
383
29.893
−19.707
17.763
1.00
20.15
A
N


ATOM
3140
CA
THR
A
383
30.333
−21.085
17.802
1.00
23.53
A
C


ATOM
3141
CB
THR
A
383
29.357
−21.983
17.034
1.00
28.25
A
C


ATOM
3142
OG1
THR
A
383
29.399
−21.622
15.645
1.00
23.26
A
O


ATOM
3143
CG2
THR
A
383
27.905
−21.865
17.595
1.00
24.27
A
C


ATOM
3144
C
THR
A
383
31.706
−21.213
17.128
1.00
25.20
A
C


ATOM
3145
O
THR
A
383
32.118
−20.315
16.401
1.00
22.28
A
O


ATOM
3146
N
PHE
A
384
32.397
−22.329
17.372
1.00
24.54
A
N


ATOM
3147
CA
PHE
A
384
33.639
−22.623
16.685
1.00
23.44
A
C


ATOM
3148
CB
PHE
A
384
34.224
−23.977
17.066
1.00
24.59
A
C


ATOM
3149
CG
PHE
A
384
35.441
−24.332
16.261
1.00
22.84
A
C


ATOM
3150
CD1
PHE
A
384
36.661
−23.788
16.571
1.00
22.31
A
C


ATOM
3151
CE1
PHE
A
384
37.787
−24.069
15.826
1.00
22.72
A
C


ATOM
3152
CZ
PHE
A
384
37.705
−24.897
14.743
1.00
23.44
A
C


ATOM
3153
CE2
PHE
A
384
36.490
−25.439
14.390
1.00
23.71
A
C


ATOM
3154
CD2
PHE
A
384
35.350
−25.152
15.155
1.00
21.29
A
C


ATOM
3155
C
PHE
A
384
33.479
−22.568
15.188
1.00
21.88
A
C


ATOM
3156
O
PHE
A
384
34.320
−21.991
14.505
1.00
20.61
A
O


ATOM
3157
N
ASP
A
385
32.456
−23.210
14.652
1.00
20.33
A
N


ATOM
3158
CA
ASP
A
385
32.313
−23.230
13.193
1.00
23.64
A
C


ATOM
3159
CB
ASP
A
385
31.123
−24.069
12.735
1.00
28.59
A
C


ATOM
3160
CG
ASP
A
385
31.343
−25.578
12.918
1.00
34.42
A
C


ATOM
3161
OD1
ASP
A
385
32.495
−26.056
12.965
1.00
30.99
A
O


ATOM
3162
OD2
ASP
A
385
30.332
−26.289
13.005
1.00
39.78
A
O


ATOM
3163
C
ASP
A
385
32.186
−21.821
12.612
1.00
24.06
A
C


ATOM
3164
O
ASP
A
385
32.748
−21.550
11.562
1.00
21.14
A
O


ATOM
3165
N
ASP
A
386
31.429
−20.951
13.269
1.00
20.63
A
N


ATOM
3166
CA
ASP
A
386
31.252
−19.587
12.770
1.00
22.72
A
C


ATOM
3167
CB
ASP
A
386
30.090
−18.890
13.457
1.00
23.71
A
C


ATOM
3168
CG
ASP
A
386
28.732
−19.369
12.936
1.00
32.71
A
C


ATOM
3169
OD1
ASP
A
386
28.706
−20.087
11.917
1.00
33.39
A
O


ATOM
3170
OD2
ASP
A
386
27.696
−19.008
13.532
1.00
37.76
A
O


ATOM
3171
C
ASP
A
386
32.525
−18.757
12.913
1.00
22.35
A
C


ATOM
3172
O
ASP
A
386
32.924
−18.085
11.951
1.00
21.45
A
O


ATOM
3173
N
TYR
A
387
33.150
−18.819
14.095
1.00
19.24
A
N


ATOM
3174
CA
TYR
A
387
34.385
−18.120
14.344
1.00
18.73
A
C


ATOM
3175
CB
TYR
A
387
34.912
−18.343
15.766
1.00
21.24
A
C


ATOM
3176
CG
TYR
A
387
36.143
−17.508
16.016
1.00
20.82
A
C


ATOM
3177
CD1
TYR
A
387
36.025
−16.151
16.308
1.00
24.25
A
C


ATOM
3178
CE1
TYR
A
387
37.148
−15.359
16.491
1.00
27.11
A
C


ATOM
3179
CZ
TYR
A
387
38.411
−15.922
16.349
1.00
24.94
A
C


ATOM
3180
OH
TYR
A
387
39.505
−15.131
16.526
1.00
25.59
A
O


ATOM
3181
CE2
TYR
A
387
38.555
−17.257
16.033
1.00
24.64
A
C


ATOM
3182
CD2
TYR
A
387
37.423
−18.044
15.861
1.00
24.24
A
C


ATOM
3183
C
TYR
A
387
35.455
−18.539
13.355
1.00
20.98
A
C


ATOM
3184
O
TYR
A
387
36.068
−17.699
12.693
1.00
15.42
A
O


ATOM
3185
N
PHE
A
388
35.679
−19.843
13.247
1.00
19.21
A
N


ATOM
3186
CA
PHE
A
388
36.772
−20.357
12.411
1.00
19.17
A
C


ATOM
3187
CB
PHE
A
388
36.991
−21.843
12.681
1.00
20.60
A
C


ATOM
3188
CG
PHE
A
388
38.101
−22.427
11.904
1.00
19.86
A
C


ATOM
3189
CD1
PHE
A
388
39.403
−22.056
12.155
1.00
21.76
A
C


ATOM
3190
CE1
PHE
A
388
40.433
−22.577
11.410
1.00
22.56
A
C


ATOM
3191
CZ
PHE
A
388
40.172
−23.493
10.430
1.00
19.58
A
C


ATOM
3192
CE2
PHE
A
388
38.899
−23.862
10.156
1.00
20.94
A
C


ATOM
3193
CD2
PHE
A
388
37.849
−23.317
10.890
1.00
20.92
A
C


ATOM
3194
C
PHE
A
388
36.539
−20.100
10.935
1.00
19.06
A
C


ATOM
3195
O
PHE
A
388
37.497
−19.788
10.188
1.00
17.17
A
O


ATOM
3196
N
GLY
A
389
35.272
−20.191
10.528
1.00
19.37
A
N


ATOM
3197
CA
GLY
A
389
34.882
−19.911
9.159
1.00
22.43
A
C


ATOM
3198
C
GLY
A
389
35.304
−18.514
8.761
1.00
19.92
A
C


ATOM
3199
O
GLY
A
389
35.929
−18.307
7.713
1.00
20.11
A
O


ATOM
3200
N
ASN
A
390
35.047
−17.560
9.644
1.00
19.07
A
N


ATOM
3201
CA
ASN
A
390
35.560
−16.207
9.435
1.00
18.25
A
C


ATOM
3202
CB
ASN
A
390
34.861
−15.268
10.395
1.00
17.19
A
C


ATOM
3203
CG
ASN
A
390
35.081
−13.821
10.048
1.00
16.60
A
C


ATOM
3204
OD1
ASN
A
390
34.530
−13.343
9.095
1.00
16.51
A
O


ATOM
3205
ND2
ASN
A
390
35.892
−13.121
10.836
1.00
13.78
A
N


ATOM
3206
C
ASN
A
390
37.098
−16.077
9.543
1.00
17.48
A
C


ATOM
3207
O
ASN
A
390
37.734
−15.345
8.758
1.00
18.21
A
O


ATOM
3208
N
ALA
A
391
37.684
−16.754
10.531
1.00
18.86
A
N


ATOM
3209
CA
ALA
A
391
39.105
−16.616
10.877
1.00
17.38
A
C


ATOM
3210
CB
ALA
A
391
39.410
−17.321
12.235
1.00
17.83
A
C


ATOM
3211
C
ALA
A
391
40.057
−17.117
9.793
1.00
16.51
A
C


ATOM
3212
O
ALA
A
391
41.133
−16.558
9.609
1.00
16.69
A
O


ATOM
3213
N
TRP
A
392
39.708
−18.166
9.070
1.00
17.06
A
N


ATOM
3214
CA
TRP
A
392
40.571
−18.582
7.987
1.00
17.60
A
C


ATOM
3215
CB
TRP
A
392
40.433
−20.048
7.559
1.00
16.27
A
C


ATOM
3216
CG
TRP
A
392
39.154
−20.481
6.953
1.00
16.13
A
C


ATOM
3217
CD1
TRP
A
392
38.125
−21.201
7.565
1.00
21.78
A
C


ATOM
3218
NE1
TRP
A
392
37.101
−21.404
6.705
1.00
19.34
A
N


ATOM
3219
CE2
TRP
A
392
37.397
−20.927
5.496
1.00
20.92
A
C


ATOM
3220
CD2
TRP
A
392
38.704
−20.278
5.591
1.00
18.15
A
C


ATOM
3221
CE3
TRP
A
392
39.256
−19.693
4.463
1.00
16.56
A
C


ATOM
3222
CZ3
TRP
A
392
38.521
−19.736
3.287
1.00
19.13
A
C


ATOM
3223
CH2
TRP
A
392
37.256
−20.360
3.222
1.00
21.25
A
C


ATOM
3224
CZ2
TRP
A
392
36.681
−20.974
4.320
1.00
21.97
A
C


ATOM
3225
C
TRP
A
392
40.507
−17.626
6.846
1.00
18.28
A
C


ATOM
3226
O
TRP
A
392
41.498
−17.497
6.152
1.00
18.17
A
O


ATOM
3227
N
LYS
A
393
39.399
−16.918
6.649
1.00
18.78
A
N


ATOM
3228
CA
ALYS
A
393
39.388
−15.804
5.681
0.50
20.43
A
C


ATOM
3229
CA
BLYS
A
393
39.371
−15.803
5.682
0.50
20.39
A
C


ATOM
3230
CB
ALYS
A
393
37.957
−15.372
5.372
0.50
20.93
A
C


ATOM
3231
CB
BLYS
A
393
37.926
−15.367
5.411
0.50
20.85
A
C


ATOM
3232
CG
ALYS
A
393
37.194
−16.439
4.616
0.50
25.28
A
C


ATOM
3233
CG
BLYS
A
393
37.100
−16.414
4.649
0.50
25.10
A
C


ATOM
3234
CD
ALYS
A
393
35.912
−15.946
3.994
0.50
25.25
A
C


ATOM
3235
CD
BLYS
A
393
35.588
−16.182
4.735
0.50
25.71
A
C


ATOM
3236
CE
ALYS
A
393
35.147
−17.080
3.303
0.50
28.64
A
C


ATOM
3237
CE
BLYS
A
393
34.747
−17.391
4.218
0.50
29.85
A
C


ATOM
3238
NZ
ALYS
A
393
33.835
−16.643
2.731
0.50
30.37
A
N


ATOM
3239
NZ
BLYS
A
393
34.371
−18.403
5.275
0.50
26.32
A
N


ATOM
3240
C
LYS
A
393
40.215
−14.594
6.157
1.00
21.19
A
C


ATOM
3241
O
LYS
A
393
40.978
−13.980
5.370
1.00
19.62
A
O


ATOM
3242
N
SER
A
394
40.081
−14.258
7.435
1.00
18.93
A
N


ATOM
3243
CA
SER
A
394
40.788
−13.129
8.020
1.00
18.32
A
C


ATOM
3244
CB
SER
A
394
40.208
−12.768
9.390
1.00
19.03
A
C


ATOM
3245
OG
SER
A
394
40.696
−13.614
10.427
1.00
21.24
A
O


ATOM
3246
C
SER
A
394
42.274
−13.343
8.152
1.00
20.59
A
C


ATOM
3247
O
SER
A
394
43.010
−12.383
8.365
1.00
18.07
A
O


ATOM
3248
N
SER
A
395
42.742
−14.588
8.032
1.00
17.06
A
N


ATOM
3249
CA
SER
A
395
44.179
−14.855
7.974
1.00
17.63
A
C


ATOM
3250
CB
SER
A
395
44.442
−16.380
7.806
1.00
18.39
A
C


ATOM
3251
OG
SER
A
395
44.064
−16.824
6.517
1.00
17.46
A
O


ATOM
3252
C
SER
A
395
44.844
−14.108
6.831
1.00
15.64
A
C


ATOM
3253
O
SER
A
395
46.028
−13.801
6.885
1.00
16.53
A
O


ATOM
3254
N
SER
A
396
44.084
−13.900
5.774
1.00
13.99
A
N


ATOM
3255
CA
SER
A
396
44.517
−13.353
4.485
1.00
16.21
A
C


ATOM
3256
CB
SER
A
396
45.480
−12.161
4.627
1.00
19.71
A
C


ATOM
3257
OG
SER
A
396
46.832
−12.545
4.880
1.00
18.63
A
O


ATOM
3258
C
SER
A
396
45.086
−14.424
3.541
1.00
18.46
A
C


ATOM
3259
O
SER
A
396
45.476
−14.115
2.407
1.00
18.03
A
O


ATOM
3260
N
GLY
A
397
45.096
−15.681
3.985
1.00
20.43
A
N


ATOM
3261
CA
GLY
A
397
45.534
−16.792
3.112
1.00
17.12
A
C


ATOM
3262
C
GLY
A
397
44.851
−16.817
1.771
1.00
18.07
A
C


ATOM
3263
O
GLY
A
397
45.497
−16.922
0.700
1.00
20.20
A
O


ATOM
3264
N
PRO
A
398
43.530
−16.729
1.775
1.00
14.60
A
N


ATOM
3265
CA
PRO
A
398
42.879
−16.744
0.495
1.00
15.16
A
C


ATOM
3266
CB
PRO
A
398
41.396
−16.743
0.852
1.00
15.38
A
C


ATOM
3267
CG
PRO
A
398
41.355
−17.315
2.211
1.00
17.75
A
C


ATOM
3268
CD
PRO
A
398
42.564
−16.797
2.877
1.00
14.99
A
C


ATOM
3269
C
PRO
A
398
43.195
−15.562
−0.417
1.00
15.54
A
C


ATOM
3270
O
PRO
A
398
43.388
−15.756
−1.632
1.00
19.95
A
O


ATOM
3271
N
LEU
A
399
43.227
−14.368
0.139
1.00
16.00
A
N


ATOM
3272
CA
LEU
A
399
43.647
−13.182
−0.626
1.00
15.47
A
C


ATOM
3273
CB
LEU
A
399
43.569
−11.934
0.257
1.00
16.63
A
C


ATOM
3274
CG
LEU
A
399
43.910
−10.633
−0.493
1.00
16.79
A
C


ATOM
3275
CD1
LEU
A
399
42.989
−9.511
−0.045
1.00
18.57
A
C


ATOM
3276
CD2
LEU
A
399
45.414
−10.269
−0.216
1.00
15.49
A
C


ATOM
3277
C
LEU
A
399
45.051
−13.336
−1.210
1.00
14.23
A
C


ATOM
3278
O
LEU
A
399
45.267
−13.094
−2.399
1.00
15.49
A
O


ATOM
3279
N
GLN
A
400
45.999
−13.803
−0.397
1.00
15.09
A
N


ATOM
3280
CA
GLN
A
400
47.363
−13.983
−0.888
1.00
17.64
A
C


ATOM
3281
CB
GLN
A
400
48.268
−14.492
0.234
1.00
20.37
A
C


ATOM
3282
CG
GLN
A
400
48.579
−13.424
1.234
1.00
23.95
A
C


ATOM
3283
CD
GLN
A
400
49.511
−13.940
2.292
1.00
27.25
A
C


ATOM
3284
OE1
GLN
A
400
50.419
−14.720
1.985
1.00
26.60
A
O


ATOM
3285
NE2
GLN
A
400
49.238
−13.594
3.557
1.00
26.70
A
N


ATOM
3286
C
GLN
A
400
47.416
−14.931
−2.047
1.00
18.18
A
C


ATOM
3287
O
GLN
A
400
48.123
−14.697
−3.043
1.00
16.77
A
O


ATOM
3288
N
LEU
A
401
46.636
−16.000
−1.944
1.00
18.75
A
N


ATOM
3289
CA
LEU
A
401
46.602
−17.008
−3.001
1.00
18.96
A
C


ATOM
3290
CB
LEU
A
401
46.059
−18.360
−2.475
1.00
16.12
A
C


ATOM
3291
CG
LEU
A
401
47.039
−18.982
−1.457
1.00
18.92
A
C


ATOM
3292
CD1
LEU
A
401
46.386
−20.203
−0.807
1.00
19.18
A
C


ATOM
3293
CD2
LEU
A
401
48.402
−19.322
−2.151
1.00
20.53
A
C


ATOM
3294
C
LEU
A
401
45.870
−16.554
−4.264
1.00
19.96
A
C


ATOM
3295
O
LEU
A
401
46.218
−17.023
−5.345
1.00
21.04
A
O


ATOM
3296
N
VAL
A
402
44.849
−15.683
−4.153
1.00
20.26
A
N


ATOM
3297
CA
VAL
A
402
44.254
−15.057
−5.356
1.00
19.68
A
C


ATOM
3298
CB
VAL
A
402
43.032
−14.169
−5.008
1.00
24.51
A
C


ATOM
3299
CG1
VAL
A
402
42.644
−13.289
−6.187
1.00
24.17
A
C


ATOM
3300
CG2
VAL
A
402
41.867
−15.025
−4.516
1.00
21.62
A
C


ATOM
3301
C
VAL
A
402
45.314
−14.226
−6.095
1.00
18.36
A
C


ATOM
3302
O
VAL
A
402
45.444
−14.323
−7.336
1.00
21.99
A
O


ATOM
3303
N
PHE
A
403
46.073
−13.399
−5.351
1.00
19.82
A
N


ATOM
3304
CA
PHE
A
403
47.158
−12.610
−5.987
1.00
18.45
A
C


ATOM
3305
CB
PHE
A
403
47.726
−11.592
−5.012
1.00
19.39
A
C


ATOM
3306
CG
PHE
A
403
46.873
−10.346
−4.913
1.00
16.10
A
C


ATOM
3307
CD1
PHE
A
403
45.717
−10.339
−4.154
1.00
14.60
A
C


ATOM
3308
CE1
PHE
A
403
44.902
−9.224
−4.075
1.00
17.25
A
C


ATOM
3309
CZ
PHE
A
403
45.257
−8.087
−4.779
1.00
17.90
A
C


ATOM
3310
CE2
PHE
A
403
46.410
−8.092
−5.581
1.00
16.80
A
C


ATOM
3311
CD2
PHE
A
403
47.197
−9.219
−5.662
1.00
17.29
A
C


ATOM
3312
C
PHE
A
403
48.246
−13.532
−6.558
1.00
23.74
A
C


ATOM
3313
O
PHE
A
403
48.750
−13.312
−7.675
1.00
19.63
A
O


ATOM
3314
N
ALA
A
404
48.593
−14.580
−5.817
1.00
20.65
A
N


ATOM
3315
CA
ALA
A
404
49.583
−15.566
−6.308
1.00
21.07
A
C


ATOM
3316
CB
ALA
A
404
49.949
−16.642
−5.195
1.00
19.70
A
C


ATOM
3317
C
ALA
A
404
49.143
−16.225
−7.610
1.00
19.16
A
C


ATOM
3318
O
ALA
A
404
49.966
−16.411
−8.526
1.00
20.37
A
O


ATOM
3319
N
TYR
A
405
47.860
−16.564
−7.714
1.00
17.02
A
N


ATOM
3320
CA
TYR
A
405
47.319
−17.163
−8.894
1.00
18.48
A
C


ATOM
3321
CB
TYR
A
405
45.804
−17.389
−8.788
1.00
18.61
A
C


ATOM
3322
CG
TYR
A
405
45.193
−17.855
−10.085
1.00
22.58
A
C


ATOM
3323
CD1
TYR
A
405
45.158
−19.214
−10.433
1.00
25.30
A
C


ATOM
3324
CE1
TYR
A
405
44.627
−19.625
−11.636
1.00
27.03
A
C


ATOM
3325
CZ
TYR
A
405
44.104
−18.691
−12.493
1.00
25.58
A
C


ATOM
3326
OH
TYR
A
405
43.547
−19.037
−13.713
1.00
26.03
A
O


ATOM
3327
CE2
TYR
A
405
44.114
−17.358
−12.143
1.00
25.76
A
C


ATOM
3328
CD2
TYR
A
405
44.640
−16.961
−10.959
1.00
23.26
A
C


ATOM
3329
C
TYR
A
405
47.652
−16.319
−10.106
1.00
24.78
A
C


ATOM
3330
O
TYR
A
405
48.141
−16.840
−11.120
1.00
21.81
A
O


ATOM
3331
N
PHE
A
406
47.401
−15.009
−10.030
1.00
19.07
A
N


ATOM
3332
CA
PHE
A
406
47.605
−14.172
−11.232
1.00
21.54
A
C


ATOM
3333
CB
PHE
A
406
46.856
−12.857
−11.077
1.00
20.15
A
C


ATOM
3334
CG
PHE
A
406
45.371
−13.037
−11.081
1.00
18.61
A
C


ATOM
3335
CD1
PHE
A
406
44.724
−13.352
−12.235
1.00
19.65
A
C


ATOM
3336
CE1
PHE
A
406
43.366
−13.523
−12.253
1.00
18.37
A
C


ATOM
3337
CZ
PHE
A
406
42.654
−13.379
−11.125
1.00
20.24
A
C


ATOM
3338
CE2
PHE
A
406
43.274
−13.065
−9.969
1.00
17.42
A
C


ATOM
3339
CD2
PHE
A
406
44.624
−12.876
−9.937
1.00
18.30
A
C


ATOM
3340
C
PHE
A
406
49.074
−13.976
−11.547
1.00
22.70
A
C


ATOM
3341
O
PHE
A
406
49.442
−13.709
−12.676
1.00
23.27
A
O


ATOM
3342
N
ALA
A
407
49.937
−14.144
−10.550
1.00
22.55
A
N


ATOM
3343
CA
ALA
A
407
51.363
−14.019
−10.772
1.00
21.36
A
C


ATOM
3344
CB
ALA
A
407
52.017
−13.537
−9.485
1.00
19.87
A
C


ATOM
3345
C
ALA
A
407
52.040
−15.321
−11.244
1.00
23.19
A
C


ATOM
3346
O
ALA
A
407
53.142
−15.272
−11.727
1.00
30.24
A
O


ATOM
3347
N
VAL
A
408
51.413
−16.481
−11.056
1.00
27.45
A
N


ATOM
3348
CA
AVAL
A
408
52.071
−17.741
−11.425
0.50
28.57
A
C


ATOM
3349
CA
BVAL
A
408
52.017
−17.787
−11.354
0.50
29.31
A
C


ATOM
3350
CB
AVAL
A
408
52.198
−18.715
−10.251
0.50
28.56
A
C


ATOM
3351
CB
BVAL
A
408
51.854
−18.730
−10.138
0.50
30.65
A
C


ATOM
3352
CG1
AVAL
A
408
52.982
−18.047
−9.141
0.50
27.11
A
C


ATOM
3353
CG1
BVAL
A
408
51.792
−20.194
−10.544
0.50
29.44
A
C


ATOM
3354
CG2
AVAL
A
408
50.843
−19.186
−9.768
0.50
24.84
A
C


ATOM
3355
CG2
BVAL
A
408
52.990
−18.493
−9.170
0.50
33.29
A
C


ATOM
3356
C
VAL
A
408
51.427
−18.421
−12.618
1.00
30.97
A
C


ATOM
3357
O
VAL
A
408
52.107
−19.095
−13.352
1.00
34.90
A
O


ATOM
3358
N
VAL
A
409
50.141
−18.201
−12.855
1.00
29.54
A
N


ATOM
3359
CA
VAL
A
409
49.475
−18.838
−13.987
1.00
38.14
A
C


ATOM
3360
CB
VAL
A
409
48.014
−19.112
−13.672
1.00
35.38
A
C


ATOM
3361
CG1
VAL
A
409
47.287
−19.625
−14.897
1.00
40.56
A
C


ATOM
3362
CG2
VAL
A
409
47.918
−20.114
−12.513
1.00
35.95
A
C


ATOM
3363
C
VAL
A
409
49.578
−17.952
−15.212
1.00
49.01
A
C


ATOM
3364
O
VAL
A
409
49.168
−16.800
−15.169
1.00
55.44
A
O


ATOM
3365
N
GLN
A
410
50.094
−18.517
−16.303
1.00
54.11
A
N


ATOM
3366
CA
GLN
A
410
50.314
−17.775
−17.555
1.00
58.29
A
C


ATOM
3367
CB
GLN
A
410
51.272
−18.540
−18.455
1.00
74.97
A
C


ATOM
3368
CG
GLN
A
410
52.676
−18.656
−17.877
1.00
104.40
A
C


ATOM
3369
CD
GLN
A
410
53.553
−19.633
−18.641
1.00
121.42
A
C


ATOM
3370
OE1
GLN
A
410
53.131
−20.746
−18.967
1.00
129.81
A
O


ATOM
3371
NE2
GLN
A
410
54.788
−19.224
−18.920
1.00
113.30
A
N


ATOM
3372
C
GLN
A
410
49.033
−17.489
−18.324
1.00
40.60
A
C


ATOM
3373
O
GLN
A
410
48.835
−16.385
−18.826
1.00
45.84
A
O


ATOM
3374
N
ASN
A
411
48.171
−18.486
−18.422
1.00
39.87
A
N


ATOM
3375
CA
ASN
A
411
46.881
−18.321
−19.069
1.00
39.51
A
C


ATOM
3376
CB
ASN
A
411
46.755
−19.283
−20.246
1.00
43.94
A
C


ATOM
3377
CG
ASN
A
411
47.619
−18.860
−21.408
1.00
45.54
A
C


ATOM
3378
OD1
ASN
A
411
47.330
−17.879
−22.071
1.00
43.48
A
O


ATOM
3379
ND2
ASN
A
411
48.706
−19.566
−21.623
1.00
47.40
A
N


ATOM
3380
C
ASN
A
411
45.777
−18.566
−18.074
1.00
41.61
A
C


ATOM
3381
O
ASN
A
411
45.513
−19.704
−17.671
1.00
36.11
A
O


ATOM
3382
N
ILE
A
412
45.089
−17.495
−17.720
1.00
37.06
A
N


ATOM
3383
CA
ILE
A
412
44.059
−17.591
−16.717
1.00
40.38
A
C


ATOM
3384
CB
ILE
A
412
43.803
−16.228
−16.041
1.00
39.90
A
C


ATOM
3385
CG1
ILE
A
412
42.967
−15.322
−16.913
1.00
41.14
A
C


ATOM
3386
CD1
ILE
A
412
42.480
−14.114
−16.169
1.00
49.82
A
C


ATOM
3387
CG2
ILE
A
412
45.146
−15.584
−15.717
1.00
37.62
A
C


ATOM
3388
C
ILE
A
412
42.832
−18.175
−17.355
1.00
38.84
A
C


ATOM
3389
O
ILE
A
412
42.647
−18.038
−18.562
1.00
37.29
A
O


ATOM
3390
N
LYS
A
413
42.037
−18.877
−16.551
1.00
35.54
A
N


ATOM
3391
CA
ALYS
A
413
40.821
−19.542
−17.017
0.50
37.84
A
C


ATOM
3392
CA
BLYS
A
413
40.815
−19.515
−17.033
0.50
37.03
A
C


ATOM
3393
CB
ALYS
A
413
40.980
−21.064
−16.928
0.50
39.81
A
C


ATOM
3394
CB
BLYS
A
413
40.974
−21.039
−17.055
0.50
37.45
A
C


ATOM
3395
CG
ALYS
A
413
42.102
−21.647
−17.800
0.50
45.41
A
C


ATOM
3396
CG
BLYS
A
413
42.303
−21.485
−17.667
0.50
41.11
A
C


ATOM
3397
CD
ALYS
A
413
42.385
−23.114
−17.437
0.50
54.08
A
C


ATOM
3398
CD
BLYS
A
413
42.279
−22.919
−18.203
0.50
47.86
A
C


ATOM
3399
CE
ALYS
A
413
43.184
−23.853
−18.515
0.50
54.67
A
C


ATOM
3400
CE
BLYS
A
413
41.782
−23.933
−17.173
0.50
48.03
A
C


ATOM
3401
NZ
ALYS
A
413
44.525
−23.255
−18.745
0.50
52.77
A
N


ATOM
3402
NZ
BLYS
A
413
40.299
−24.089
−17.187
0.50
44.70
A
N


ATOM
3403
C
LYS
A
413
39.644
−19.071
−16.167
1.00
37.05
A
C


ATOM
3404
O
LYS
A
413
39.757
−18.944
−14.934
1.00
28.26
A
O


ATOM
3405
N
LYS
A
414
38.522
−18.795
−16.818
1.00
32.05
A
N


ATOM
3406
CA
LYS
A
414
37.363
−18.243
−16.148
1.00
35.17
A
C


ATOM
3407
CB
LYS
A
414
36.244
−17.952
−17.162
1.00
42.50
A
C


ATOM
3408
CG
LYS
A
414
34.967
−17.374
−16.532
1.00
58.00
A
C


ATOM
3409
CD
LYS
A
414
33.942
−16.848
−17.562
1.00
70.13
A
C


ATOM
3410
CE
LYS
A
414
33.549
−17.894
−18.610
1.00
76.49
A
C


ATOM
3411
NZ
LYS
A
414
33.122
−19.206
−18.034
1.00
79.30
A
N


ATOM
3412
C
LYS
A
414
36.869
−19.184
−15.049
1.00
30.94
A
C


ATOM
3413
O
LYS
A
414
36.458
−18.749
−13.978
1.00
33.29
A
O


ATOM
3414
N
GLU
A
415
36.936
−20.475
−15.309
1.00
29.17
A
N


ATOM
3415
CA
GLU
A
415
36.503
−21.464
−14.340
1.00
29.56
A
C


ATOM
3416
CB
GLU
A
415
36.455
−22.821
−15.005
1.00
36.10
A
C


ATOM
3417
CG
GLU
A
415
35.755
−23.839
−14.185
1.00
47.28
A
C


ATOM
3418
CD
GLU
A
415
35.920
−25.229
−14.755
1.00
65.80
A
C


ATOM
3419
OE1
GLU
A
415
36.443
−25.354
−15.885
1.00
99.10
A
O


ATOM
3420
OE2
GLU
A
415
35.539
−26.194
−14.066
1.00
53.16
A
O


ATOM
3421
C
GLU
A
415
37.409
−21.520
−13.102
1.00
26.70
A
C


ATOM
3422
O
GLU
A
415
36.934
−21.688
−11.975
1.00
27.05
A
O


ATOM
3423
N
GLU
A
416
38.704
−21.379
−13.315
1.00
22.01
A
N


ATOM
3424
CA
GLU
A
416
39.636
−21.231
−12.204
1.00
28.96
A
C


ATOM
3425
CB
GLU
A
416
41.059
−21.196
−12.729
1.00
29.30
A
C


ATOM
3426
CG
GLU
A
416
41.445
−22.546
−13.360
1.00
35.82
A
C


ATOM
3427
CD
GLU
A
416
42.902
−22.633
−13.766
1.00
38.67
A
C


ATOM
3428
OE1
GLU
A
416
43.543
−21.587
−14.001
1.00
34.53
A
O


ATOM
3429
OE2
GLU
A
416
43.410
−23.762
−13.857
1.00
49.04
A
O


ATOM
3430
C
GLU
A
416
39.323
−20.006
−11.368
1.00
24.91
A
C


ATOM
3431
O
GLU
A
416
39.190
−20.087
−10.150
1.00
25.36
A
O


ATOM
3432
N
ILE
A
417
39.162
−18.864
−12.013
1.00
25.14
A
N


ATOM
3433
CA
AILE
A
417
38.954
−17.658
−11.222
0.50
25.62
A
C


ATOM
3434
CA
BILE
A
417
38.864
−17.587
−11.339
0.50
27.40
A
C


ATOM
3435
CB
AILE
A
417
39.296
−16.353
−11.998
0.50
25.97
A
C


ATOM
3436
CB
BILE
A
417
38.703
−16.454
−12.392
0.50
28.46
A
C


ATOM
3437
CG1
AILE
A
417
38.397
−16.153
−13.210
0.50
24.13
A
C


ATOM
3438
CG1
BILE
A
417
40.059
−16.125
−13.015
0.50
28.41
A
C


ATOM
3439
CD1
AILE
A
417
38.647
−14.818
−13.903
0.50
31.10
A
C


ATOM
3440
CD1
BILE
A
417
39.965
−15.233
−14.243
0.50
32.20
A
C


ATOM
3441
CG2
AILE
A
417
40.755
−16.378
−12.431
0.50
21.28
A
C


ATOM
3442
CG2
BILE
A
417
38.081
−15.207
−11.771
0.50
26.97
A
C


ATOM
3443
C
ILE
A
417
37.573
−17.670
−10.532
1.00
26.52
A
C


ATOM
3444
O
ILE
A
417
37.487
−17.251
−9.379
1.00
29.89
A
O


ATOM
3445
N
GLU
A
418
36.544
−18.217
−11.169
1.00
26.91
A
N


ATOM
3446
CA
GLU
A
418
35.234
−18.397
−10.519
1.00
28.93
A
C


ATOM
3447
CB
GLU
A
418
34.231
−19.058
−11.472
1.00
34.19
A
C


ATOM
3448
CG
GLU
A
418
33.560
−18.105
−12.449
1.00
53.05
A
C


ATOM
3449
CD
GLU
A
418
32.603
−18.811
−13.425
1.00
66.68
A
C


ATOM
3450
OE1
GLU
A
418
32.687
−20.057
−13.603
1.00
57.67
A
O


ATOM
3451
OE2
GLU
A
418
31.765
−18.102
−14.025
1.00
78.77
A
O


ATOM
3452
C
GLU
A
418
35.342
−19.259
−9.247
1.00
30.46
A
C


ATOM
3453
O
GLU
A
418
34.675
−18.996
−8.248
1.00
32.98
A
O


ATOM
3454
N
ASN
A
419
36.185
−20.282
−9.295
1.00
25.76
A
N


ATOM
3455
CA
ASN
A
419
36.413
−21.134
−8.131
1.00
27.38
A
C


ATOM
3456
CB
ASN
A
419
37.004
−22.473
−8.579
1.00
28.62
A
C


ATOM
3457
CG
ASN
A
419
35.920
−23.449
−8.982
1.00
33.85
A
C


ATOM
3458
OD1
ASN
A
419
35.304
−24.059
−8.127
1.00
32.06
A
O


ATOM
3459
ND2
ASN
A
419
35.621
−23.527
−10.272
1.00
29.04
A
N


ATOM
3460
C
ASN
A
419
37.241
−20.483
−7.021
1.00
21.01
A
C


ATOM
3461
O
ASN
A
419
37.001
−20.725
−5.855
1.00
24.32
A
O


ATOM
3462
N
LEU
A
420
38.171
−19.621
−7.392
1.00
23.30
A
N


ATOM
3463
CA
LEU
A
420
38.880
−18.803
−6.417
1.00
26.86
A
C


ATOM
3464
CB
LEU
A
420
39.939
−17.948
−7.094
1.00
30.50
A
C


ATOM
3465
CG
LEU
A
420
41.192
−18.585
−7.691
1.00
29.17
A
C


ATOM
3466
CD1
LEU
A
420
41.932
−17.455
−8.424
1.00
29.98
A
C


ATOM
3467
CD2
LEU
A
420
42.052
−19.214
−6.627
1.00
27.85
A
C


ATOM
3468
C
LEU
A
420
37.894
−17.914
−5.655
1.00
27.14
A
C


ATOM
3469
O
LEU
A
420
37.968
−17.811
−4.438
1.00
27.87
A
O


ATOM
3470
N
GLN
A
421
36.925
−17.344
−6.357
1.00
28.53
A
N


ATOM
3471
CA
GLN
A
421
35.952
−16.477
−5.710
1.00
36.09
A
C


ATOM
3472
CB
GLN
A
421
35.090
−15.747
−6.726
1.00
38.74
A
C


ATOM
3473
CG
GLN
A
421
35.881
−14.837
−7.596
1.00
41.97
A
C


ATOM
3474
CD
GLN
A
421
35.071
−13.634
−8.005
1.00
51.05
A
C


ATOM
3475
OE1
GLN
A
421
34.064
−13.769
−8.686
1.00
42.51
A
O


ATOM
3476
NE2
GLN
A
421
35.500
−12.455
−7.582
1.00
34.40
A
N


ATOM
3477
C
GLN
A
421
35.034
−17.200
−4.765
1.00
32.47
A
C


ATOM
3478
O
GLN
A
421
34.573
−16.616
−3.795
1.00
27.79
A
O


ATOM
3479
N
LYS
A
422
34.782
−18.476
−5.025
1.00
25.83
A
N


ATOM
3480
CA
LYS
A
422
34.003
−19.278
−4.115
1.00
22.56
A
C


ATOM
3481
CB
LYS
A
422
33.154
−20.284
−4.923
1.00
33.44
A
C


ATOM
3482
CG
LYS
A
422
32.134
−19.596
−5.816
1.00
45.98
A
C


ATOM
3483
CD
LYS
A
422
31.728
−20.416
−7.049
1.00
63.36
A
C


ATOM
3484
CE
LYS
A
422
30.833
−19.557
−7.974
1.00
73.27
A
C


ATOM
3485
NZ
LYS
A
422
30.447
−20.198
−9.275
1.00
66.66
A
N


ATOM
3486
C
LYS
A
422
34.880
−20.014
−3.107
1.00
23.96
A
C


ATOM
3487
O
LYS
A
422
34.412
−20.944
−2.457
1.00
25.32
A
O


ATOM
3488
N
TYR
A
423
36.148
−19.633
−2.965
1.00
24.30
A
N


ATOM
3489
CA
TYR
A
423
37.034
−20.295
−1.983
1.00
24.51
A
C


ATOM
3490
CB
TYR
A
423
36.551
−20.040
−0.567
1.00
27.30
A
C


ATOM
3491
CG
TYR
A
423
36.497
−18.583
−0.259
1.00
30.64
A
C


ATOM
3492
CD1
TYR
A
423
37.628
−17.915
0.119
1.00
32.19
A
C


ATOM
3493
CE1
TYR
A
423
37.596
−16.571
0.425
1.00
40.57
A
C


ATOM
3494
CZ
TYR
A
423
36.408
−15.873
0.331
1.00
45.26
A
C


ATOM
3495
OH
TYR
A
423
36.397
−14.533
0.619
1.00
45.20
A
O


ATOM
3496
CE2
TYR
A
423
35.254
−16.513
−0.057
1.00
38.81
A
C


ATOM
3497
CD2
TYR
A
423
35.301
−17.874
−0.349
1.00
43.23
A
C


ATOM
3498
C
TYR
A
423
37.169
−21.796
−2.162
1.00
23.71
A
C


ATOM
3499
O
TYR
A
423
36.979
−22.565
−1.223
1.00
23.89
A
O


ATOM
3500
N
HIS
A
424
37.537
−22.204
−3.365
1.00
22.42
A
N


ATOM
3501
CA
HIS
A
424
37.810
−23.617
−3.654
1.00
25.69
A
C


ATOM
3502
CB
HIS
A
424
38.434
−23.688
−5.041
1.00
28.03
A
C


ATOM
3503
CG
HIS
A
424
38.579
−25.068
−5.589
1.00
26.61
A
C


ATOM
3504
ND1
HIS
A
424
39.719
−25.801
−5.448
1.00
26.17
A
N


ATOM
3505
CE1
HIS
A
424
39.554
−27.000
−6.057
1.00
33.18
A
C


ATOM
3506
NE2
HIS
A
424
38.346
−27.003
−6.639
1.00
30.75
A
N


ATOM
3507
CD2
HIS
A
424
37.721
−25.819
−6.366
1.00
27.10
A
C


ATOM
3508
C
HIS
A
424
38.776
−24.183
−2.649
1.00
24.11
A
C


ATOM
3509
O
HIS
A
424
39.680
−23.484
−2.130
1.00
19.88
A
O


ATOM
3510
N
ASP
A
425
38.640
−25.478
−2.388
1.00
23.19
A
N


ATOM
3511
CA
ASP
A
425
39.546
−26.229
−1.525
1.00
24.75
A
C


ATOM
3512
CB
ASP
A
425
39.349
−27.730
−1.751
1.00
28.91
A
C


ATOM
3513
CG
ASP
A
425
37.961
−28.223
−1.351
1.00
34.79
A
C


ATOM
3514
OD1
ASP
A
425
37.338
−27.632
−0.461
1.00
38.40
A
O


ATOM
3515
OD2
ASP
A
425
37.510
−29.218
−1.954
1.00
42.28
A
O


ATOM
3516
C
ASP
A
425
41.041
−25.973
−1.709
1.00
21.70
A
C


ATOM
3517
O
ASP
A
425
41.813
−26.049
−0.766
1.00
20.92
A
O


ATOM
3518
N
THR
A
426
41.462
−25.772
−2.943
1.00
19.82
A
N


ATOM
3519
CA
THR
A
426
42.836
−25.508
−3.232
1.00
19.64
A
C


ATOM
3520
CB
THR
A
426
42.974
−25.165
−4.734
1.00
21.99
A
C


ATOM
3521
OG1
THR
A
426
42.497
−26.284
−5.475
1.00
31.41
A
O


ATOM
3522
CG2
THR
A
426
44.411
−24.887
−5.087
1.00
21.42
A
C


ATOM
3523
C
THR
A
426
43.366
−24.323
−2.407
1.00
20.34
A
C


ATOM
3524
O
THR
A
426
44.529
−24.327
−2.031
1.00
20.11
A
O


ATOM
3525
N
ILE
A
427
42.538
−23.284
−2.203
1.00
18.12
A
N


ATOM
3526
CA
ILE
A
427
42.955
−22.142
−1.396
1.00
17.33
A
C


ATOM
3527
CB
ILE
A
427
42.765
−20.794
−2.111
1.00
21.38
A
C


ATOM
3528
CG1
ILE
A
427
41.327
−20.360
−2.144
1.00
18.39
A
C


ATOM
3529
CD1
ILE
A
427
41.189
−18.919
−2.801
1.00
20.52
A
C


ATOM
3530
CG2
ILE
A
427
43.361
−20.870
−3.526
1.00
19.29
A
C


ATOM
3531
C
ILE
A
427
42.413
−22.102
0.010
1.00
16.88
A
C


ATOM
3532
O
ILE
A
427
43.095
−21.588
0.917
1.00
20.78
A
O


ATOM
3533
N
SER
A
428
41.287
−22.761
0.268
1.00
16.24
A
N


ATOM
3534
CA
SER
A
428
40.728
−22.677
1.587
1.00
18.26
A
C


ATOM
3535
CB
SER
A
428
39.260
−23.061
1.561
1.00
22.20
A
C


ATOM
3536
OG
SER
A
428
39.129
−24.406
1.151
1.00
23.46
A
O


ATOM
3537
C
SER
A
428
41.525
−23.567
2.547
1.00
19.47
A
C


ATOM
3538
O
SER
A
428
41.822
−23.181
3.671
1.00
18.98
A
O


ATOM
3539
N
ARG
A
429
41.929
−24.754
2.119
1.00
19.92
A
N


ATOM
3540
CA
AARG
A
429
42.582
−25.692
3.049
0.50
17.38
A
C


ATOM
3541
CA
BARG
A
429
42.548
−25.696
3.052
0.50
18.39
A
C


ATOM
3542
CB
AARG
A
429
42.756
−27.069
2.409
0.50
19.41
A
C


ATOM
3543
CB
BARG
A
429
42.596
−27.113
2.430
0.50
22.82
A
C


ATOM
3544
CG
AARG
A
429
41.488
−27.872
2.518
0.50
19.06
A
C


ATOM
3545
CG
BARG
A
429
41.176
−27.620
1.991
0.50
22.48
A
C


ATOM
3546
CD
AARG
A
429
41.548
−28.993
1.550
0.50
22.31
A
C


ATOM
3547
CD
BARG
A
429
40.778
−28.905
2.690
0.50
31.48
A
C


ATOM
3548
NE
AARG
A
429
40.271
−29.672
1.469
0.50
23.52
A
N


ATOM
3549
NE
BARG
A
429
39.575
−29.554
2.140
0.50
30.27
A
N


ATOM
3550
CZ
AARG
A
429
39.930
−30.402
0.439
0.50
15.63
A
C


ATOM
3551
CZ
BARG
A
429
38.399
−29.587
2.763
0.50
34.86
A
C


ATOM
3552
NH1
AARG
A
429
40.782
−30.524
−0.568
0.50
19.41
A
N


ATOM
3553
NH1
BARG
A
429
38.279
−29.014
3.956
0.50
26.08
A
N


ATOM
3554
NH2
AARG
A
429
38.776
−30.963
0.435
0.50
17.28
A
N


ATOM
3555
NH2
BARG
A
429
37.361
−30.237
2.218
0.50
27.64
A
N


ATOM
3556
C
ARG
A
429
43.918
−25.198
3.588
1.00
16.22
A
C


ATOM
3557
O
ARG
A
429
44.183
−25.282
4.780
1.00
16.19
A
O


ATOM
3558
N
PRO
A
430
44.762
−24.642
2.726
1.00
16.98
A
N


ATOM
3559
CA
PRO
A
430
46.018
−24.145
3.283
1.00
19.48
A
C


ATOM
3560
CB
PRO
A
430
46.830
−23.764
2.056
1.00
24.27
A
C


ATOM
3561
CG
PRO
A
430
45.963
−23.936
0.930
1.00
26.12
A
C


ATOM
3562
CD
PRO
A
430
44.812
−24.737
1.263
1.00
23.07
A
C


ATOM
3563
C
PRO
A
430
45.778
−22.938
4.192
1.00
17.44
A
C


ATOM
3564
O
PRO
A
430
46.536
−22.713
5.146
1.00
16.01
A
O


ATOM
3565
N
SER
A
431
44.699
−22.214
3.936
1.00
16.23
A
N


ATOM
3566
CA
SER
A
431
44.291
−21.083
4.806
1.00
16.47
A
C


ATOM
3567
CB
SER
A
431
43.250
−20.232
4.083
1.00
17.77
A
C


ATOM
3568
OG
SER
A
431
43.801
−19.694
2.865
1.00
18.39
A
O


ATOM
3569
C
SER
A
431
43.777
−21.582
6.164
1.00
18.22
A
C


ATOM
3570
O
SER
A
431
43.993
−20.908
7.174
1.00
18.59
A
O


ATOM
3571
N
HIS
A
432
43.156
−22.780
6.204
1.00
15.86
A
N


ATOM
3572
CA
HIS
A
432
42.780
−23.405
7.448
1.00
16.36
A
C


ATOM
3573
CB
HIS
A
432
42.110
−24.770
7.287
1.00
16.77
A
C


ATOM
3574
CG
HIS
A
432
40.776
−24.777
6.567
1.00
20.10
A
C


ATOM
3575
ND1
HIS
A
432
40.132
−23.664
6.137
1.00
25.10
A
N


ATOM
3576
CE1
HIS
A
432
38.961
−24.023
5.537
1.00
20.22
A
C


ATOM
3577
NE2
HIS
A
432
38.867
−25.348
5.579
1.00
27.66
A
N


ATOM
3578
CD2
HIS
A
432
39.974
−25.846
6.202
1.00
23.79
A
C


ATOM
3579
C
HIS
A
432
44.030
−23.620
8.282
1.00
14.57
A
C


ATOM
3580
O
HIS
A
432
44.070
−23.311
9.467
1.00
17.94
A
O


ATOM
3581
N
ILE
A
433
45.067
−24.158
7.661
1.00
15.54
A
N


ATOM
3582
CA
ILE
A
433
46.306
−24.452
8.352
1.00
16.10
A
C


ATOM
3583
CB
ILE
A
433
47.233
−25.278
7.483
1.00
17.95
A
C


ATOM
3584
CG1
ILE
A
433
46.632
−26.708
7.366
1.00
20.19
A
C


ATOM
3585
CD1
ILE
A
433
47.196
−27.406
6.192
1.00
26.65
A
C


ATOM
3586
CG2
ILE
A
433
48.609
−25.425
8.153
1.00
16.48
A
C


ATOM
3587
C
ILE
A
433
47.017
−23.187
8.812
1.00
17.80
A
C


ATOM
3588
O
ILE
A
433
47.544
−23.147
9.920
1.00
19.64
A
O


ATOM
3589
N
PHE
A
434
47.023
−22.176
7.963
1.00
17.18
A
N


ATOM
3590
CA
PHE
A
434
47.604
−20.870
8.304
1.00
17.38
A
C


ATOM
3591
CB
PHE
A
434
47.400
−19.972
7.085
1.00
19.35
A
C


ATOM
3592
CG
PHE
A
434
47.927
−18.537
7.224
1.00
19.98
A
C


ATOM
3593
CD1
PHE
A
434
48.715
−18.114
8.279
1.00
22.63
A
C


ATOM
3594
CE1
PHE
A
434
49.148
−16.776
8.353
1.00
24.62
A
C


ATOM
3595
CZ
PHE
A
434
48.870
−15.921
7.348
1.00
20.63
A
C


ATOM
3596
CE2
PHE
A
434
48.102
−16.345
6.274
1.00
24.11
A
C


ATOM
3597
CD2
PHE
A
434
47.651
−17.633
6.219
1.00
23.72
A
C


ATOM
3598
C
PHE
A
434
46.959
−20.325
9.604
1.00
18.74
A
C


ATOM
3599
O
PHE
A
434
47.659
−19.969
10.581
1.00
18.92
A
O


ATOM
3600
N
ARG
A
435
45.632
−20.304
9.645
1.00
17.74
A
N


ATOM
3601
CA
ARG
A
435
44.922
−19.799
10.824
1.00
16.89
A
C


ATOM
3602
CB
ARG
A
435
43.436
−19.607
10.511
1.00
15.62
A
C


ATOM
3603
CG
ARG
A
435
42.545
−19.325
11.731
1.00
15.59
A
C


ATOM
3604
CD
ARG
A
435
43.046
−18.111
12.556
1.00
17.62
A
C


ATOM
3605
NE
ARG
A
435
42.921
−16.870
11.800
1.00
19.60
A
N


ATOM
3606
CZ
ARG
A
435
43.489
−15.717
12.143
1.00
20.76
A
C


ATOM
3607
NH1
ARG
A
435
44.232
−15.628
13.228
1.00
20.32
A
N


ATOM
3608
NH2
ARG
A
435
43.277
−14.639
11.409
1.00
20.79
A
N


ATOM
3609
C
ARG
A
435
45.162
−20.661
12.053
1.00
18.87
A
C


ATOM
3610
O
ARG
A
435
45.423
−20.145
13.168
1.00
18.81
A
O


ATOM
3611
N
LEU
A
436
45.152
−21.979
11.871
1.00
17.30
A
N


ATOM
3612
CA
LEU
A
436
45.320
−22.879
13.015
1.00
18.57
A
C


ATOM
3613
CB
LEU
A
436
44.898
−24.321
12.637
1.00
18.56
A
C


ATOM
3614
CG
LEU
A
436
43.378
−24.533
12.413
1.00
20.39
A
C


ATOM
3615
CD1
LEU
A
436
43.085
−25.939
11.814
1.00
19.89
A
C


ATOM
3616
CD2
LEU
A
436
42.511
−24.299
13.677
1.00
19.00
A
C


ATOM
3617
C
LEU
A
436
46.734
−22.825
13.616
1.00
18.56
A
C


ATOM
3618
O
LEU
A
436
46.890
−22.862
14.826
1.00
22.85
A
O


ATOM
3619
N
CYS
A
437
47.752
−22.733
12.771
1.00
18.27
A
N


ATOM
3620
CA
CYS
A
437
49.124
−22.609
13.221
1.00
18.06
A
C


ATOM
3621
CB
CYS
A
437
50.051
−22.581
12.031
1.00
20.66
A
C


ATOM
3622
SG
CYS
A
437
50.305
−24.213
11.297
1.00
24.31
A
S


ATOM
3623
C
CYS
A
437
49.300
−21.305
14.012
1.00
20.39
A
C


ATOM
3624
O
CYS
A
437
49.927
−21.286
15.042
1.00
23.46
A
O


ATOM
3625
N
ASN
A
438
48.738
−20.233
13.487
1.00
18.39
A
N


ATOM
3626
CA
ASN
A
438
48.801
−18.903
14.099
1.00
19.69
A
C


ATOM
3627
CB
ASN
A
438
48.116
−17.871
13.172
1.00
19.76
A
C


ATOM
3628
CG
ASN
A
438
48.286
−16.435
13.678
1.00
27.64
A
C


ATOM
3629
OD1
ASN
A
438
48.974
−16.224
14.631
1.00
29.01
A
O


ATOM
3630
ND2
ASN
A
438
47.730
−15.473
12.988
1.00
23.97
A
N


ATOM
3631
C
ASN
A
438
48.120
−18.944
15.451
1.00
21.36
A
C


ATOM
3632
O
ASN
A
438
48.694
−18.536
16.480
1.00
23.50
A
O


ATOM
3633
N
ASP
A
439
46.902
−19.473
15.473
1.00
19.55
A
N


ATOM
3634
CA
ASP
A
439
46.146
−19.487
16.702
1.00
19.28
A
C


ATOM
3635
CB
ASP
A
439
44.661
−19.756
16.448
1.00
19.28
A
C


ATOM
3636
CG
ASP
A
439
43.932
−18.546
15.925
1.00
21.53
A
C


ATOM
3637
OD1
ASP
A
439
44.580
−17.555
15.494
1.00
19.39
A
O


ATOM
3638
OD2
ASP
A
439
42.672
−18.593
15.921
1.00
23.22
A
O


ATOM
3639
C
ASP
A
439
46.732
−20.438
17.731
1.00
22.35
A
C


ATOM
3640
O
ASP
A
439
46.658
−20.158
18.952
1.00
24.01
A
O


ATOM
3641
N
LEU
A
440
47.316
−21.547
17.286
1.00
22.14
A
N


ATOM
3642
CA
LEU
A
440
48.029
−22.441
18.221
1.00
23.93
A
C


ATOM
3643
CB
LEU
A
440
48.523
−23.732
17.555
1.00
23.86
A
C


ATOM
3644
CG
LEU
A
440
47.537
−24.862
17.307
1.00
25.59
A
C


ATOM
3645
CD1
LEU
A
440
48.140
−25.874
16.284
1.00
22.82
A
C


ATOM
3646
CD2
LEU
A
440
47.097
−25.559
18.609
1.00
21.71
A
C


ATOM
3647
C
LEU
A
440
49.218
−21.755
18.890
1.00
27.52
A
C


ATOM
3648
O
LEU
A
440
49.452
−21.962
20.077
1.00
26.63
A
O


ATOM
3649
N
ALA
A
441
49.969
−20.967
18.123
1.00
26.98
A
N


ATOM
3650
CA
ALA
A
441
51.138
−20.229
18.651
1.00
28.39
A
C


ATOM
3651
CB
ALA
A
441
51.774
−19.412
17.571
1.00
25.07
A
C


ATOM
3652
C
ALA
A
441
50.786
−19.306
19.815
1.00
31.85
A
C


ATOM
3653
O
ALA
A
441
51.517
−19.248
20.779
1.00
30.87
A
O


ATOM
3654
N
SER
A
442
49.654
−18.613
19.737
1.00
34.55
A
N


ATOM
3655
CA
SER
A
442
49.272
−17.614
20.753
1.00
31.17
A
C


ATOM
3656
CB
SER
A
442
48.827
−16.328
20.058
1.00
32.51
A
C


ATOM
3657
OG
SER
A
442
47.744
−16.577
19.187
1.00
37.82
A
O


ATOM
3658
C
SER
A
442
48.178
−18.061
21.716
1.00
32.18
A
C


ATOM
3659
O
SER
A
442
47.830
−17.330
22.649
1.00
37.74
A
O


ATOM
3660
N
ALA
A
443
47.658
−19.268
21.535
1.00
26.46
A
N


ATOM
3661
CA
ALA
A
443
46.509
−19.710
22.326
1.00
25.17
A
C


ATOM
3662
CB
ALA
A
443
46.080
−21.107
21.915
1.00
26.66
A
C


ATOM
3663
C
ALA
A
443
46.758
−19.676
23.845
1.00
28.97
A
C


ATOM
3664
O
ALA
A
443
45.877
−19.257
24.585
1.00
27.44
A
O


ATOM
3665
N
SER
A
444
47.924
−20.138
24.301
1.00
29.81
A
N


ATOM
3666
CA
SER
A
444
48.189
−20.243
25.761
1.00
38.24
A
C


ATOM
3667
CB
SER
A
444
49.524
−20.940
26.042
1.00
44.57
A
C


ATOM
3668
OG
SER
A
444
49.417
−22.333
25.821
1.00
50.77
A
O


ATOM
3669
C
SER
A
444
48.176
−18.886
26.459
1.00
31.44
A
C


ATOM
3670
O
SER
A
444
47.522
−18.717
27.486
1.00
42.99
A
O


ATOM
3671
N
ALA
A
445
48.910
−17.940
25.893
1.00
31.00
A
N


ATOM
3672
CA
ALA
A
445
48.970
−16.578
26.389
1.00
37.39
A
C


ATOM
3673
CB
ALA
A
445
49.981
−15.766
25.579
1.00
31.84
A
C


ATOM
3674
C
ALA
A
445
47.595
−15.905
26.358
1.00
45.10
A
C


ATOM
3675
O
ALA
A
445
47.218
−15.249
27.318
1.00
37.87
A
O


ATOM
3676
N
GLU
A
446
46.842
−16.071
25.272
1.00
31.27
A
N


ATOM
3677
CA
GLU
A
446
45.536
−15.411
25.147
1.00
33.60
A
C


ATOM
3678
CB
GLU
A
446
45.028
−15.489
23.704
1.00
38.49
A
C


ATOM
3679
CG
GLU
A
446
45.802
−14.564
22.754
1.00
40.53
A
C


ATOM
3680
CD
GLU
A
446
45.507
−14.810
21.278
1.00
47.65
A
C


ATOM
3681
OE1
GLU
A
446
44.636
−15.647
20.955
1.00
46.48
A
O


ATOM
3682
OE2
GLU
A
446
46.166
−14.164
20.429
1.00
54.74
A
O


ATOM
3683
C
GLU
A
446
44.496
−15.971
26.108
1.00
33.60
A
C


ATOM
3684
O
GLU
A
446
43.726
−15.224
26.716
1.00
35.20
A
O


ATOM
3685
N
ILE
A
447
44.473
−17.290
26.222
1.00
30.36
A
N


ATOM
3686
CA
ILE
A
447
43.622
−17.986
27.168
1.00
33.01
A
C


ATOM
3687
CB
ILE
A
447
43.782
−19.509
27.020
1.00
33.51
A
C


ATOM
3688
CG1
ILE
A
447
43.047
−19.991
25.764
1.00
36.07
A
C


ATOM
3689
CD1
ILE
A
447
43.514
−21.342
25.254
1.00
32.96
A
C


ATOM
3690
CG2
ILE
A
447
43.290
−20.243
28.265
1.00
28.15
A
C


ATOM
3691
C
ILE
A
447
43.957
−17.592
28.618
1.00
41.30
A
C


ATOM
3692
O
ILE
A
447
43.061
−17.514
29.451
1.00
35.18
A
O


ATOM
3693
N
ALA
A
448
45.241
−17.358
28.900
1.00
35.98
A
N


ATOM
3694
CA
ALA
A
448
45.701
−16.935
30.232
1.00
39.88
A
C


ATOM
3695
CB
ALA
A
448
47.227
−17.079
30.326
1.00
38.54
A
C


ATOM
3696
C
ALA
A
448
45.281
−15.498
30.575
1.00
41.45
A
C


ATOM
3697
O
ALA
A
448
45.055
−15.183
31.734
1.00
49.52
A
O


ATOM
3698
N
ARG
A
449
45.180
−14.633
29.568
1.00
43.05
A
N


ATOM
3699
CA
ARG
A
449
44.694
−13.266
29.752
1.00
37.79
A
C


ATOM
3700
CB
ARG
A
449
45.231
−12.346
28.651
1.00
45.66
A
C


ATOM
3701
CG
ARG
A
449
46.748
−12.245
28.560
1.00
48.52
A
C


ATOM
3702
CD
ARG
A
449
47.155
−11.295
27.435
1.00
52.98
A
C


ATOM
3703
NE
ARG
A
449
48.158
−11.903
26.560
1.00
65.22
A
N


ATOM
3704
CZ
ARG
A
449
48.074
−12.008
25.230
1.00
67.12
A
C


ATOM
3705
NH1
ARG
A
449
47.034
−11.523
24.553
1.00
54.25
A
N


ATOM
3706
NH2
ARG
A
449
49.058
−12.601
24.556
1.00
72.81
A
N


ATOM
3707
C
ARG
A
449
43.163
−13.183
29.734
1.00
41.63
A
C


ATOM
3708
O
ARG
A
449
42.606
−12.085
29.794
1.00
38.89
A
O


ATOM
3709
N
GLY
A
450
42.484
−14.325
29.641
1.00
36.28
A
N


ATOM
3710
CA
GLY
A
450
41.026
−14.357
29.604
1.00
36.62
A
C


ATOM
3711
C
GLY
A
450
40.380
−14.056
28.259
1.00
42.78
A
C


ATOM
3712
O
GLY
A
450
39.166
−13.923
28.186
1.00
43.69
A
O


ATOM
3713
N
GLU
A
451
41.182
−13.961
27.198
1.00
47.14
A
N


ATOM
3714
CA
GLU
A
451
40.686
−13.682
25.848
1.00
43.60
A
C


ATOM
3715
CB
GLU
A
451
41.829
−13.194
24.959
1.00
44.90
A
C


ATOM
3716
CG
GLU
A
451
42.482
−11.927
25.491
1.00
54.21
A
C


ATOM
3717
CD
GLU
A
451
43.585
−11.404
24.600
1.00
62.45
A
C


ATOM
3718
OE1
GLU
A
451
43.462
−11.514
23.355
1.00
60.04
A
O


ATOM
3719
OE2
GLU
A
451
44.574
−10.876
25.154
1.00
64.85
A
O


ATOM
3720
C
GLU
A
451
40.031
−14.898
25.198
1.00
41.18
A
C


ATOM
3721
O
GLU
A
451
40.341
−16.034
25.534
1.00
44.70
A
O


ATOM
3722
N
THR
A
452
39.119
−14.637
24.267
1.00
33.47
A
N


ATOM
3723
CA
THR
A
452
38.338
−15.687
23.611
1.00
32.68
A
C


ATOM
3724
CB
THR
A
452
36.834
−15.582
23.983
1.00
33.03
A
C


ATOM
3725
OG1
THR
A
452
36.309
−14.334
23.509
1.00
34.61
A
O


ATOM
3726
CG2
THR
A
452
36.623
−15.701
25.502
1.00
29.34
A
C


ATOM
3727
C
THR
A
452
38.442
−15.658
22.077
1.00
27.61
A
C


ATOM
3728
O
THR
A
452
37.873
−16.518
21.417
1.00
35.74
A
O


ATOM
3729
N
ALA
A
453
39.153
−14.672
21.533
1.00
27.23
A
N


ATOM
3730
CA
ALA
A
453
39.261
−14.441
20.103
1.00
28.56
A
C


ATOM
3731
CB
ALA
A
453
39.508
−12.945
19.807
1.00
24.78
A
C


ATOM
3732
C
ALA
A
453
40.389
−15.278
19.550
1.00
28.41
A
C


ATOM
3733
O
ALA
A
453
41.394
−14.745
19.086
1.00
30.61
A
O


ATOM
3734
N
ASN
A
454
40.210
−16.594
19.599
1.00
26.38
A
N


ATOM
3735
CA
ASN
A
454
41.236
−17.554
19.193
1.00
25.56
A
C


ATOM
3736
CB
ASN
A
454
42.239
−17.757
20.338
1.00
22.98
A
C


ATOM
3737
CG
ASN
A
454
43.376
−18.724
19.989
1.00
24.74
A
C


ATOM
3738
OD1
ASN
A
454
43.207
−19.938
20.050
1.00
30.88
A
O


ATOM
3739
ND2
ASN
A
454
44.553
−18.187
19.677
1.00
22.05
A
N


ATOM
3740
C
ASN
A
454
40.533
−18.863
18.842
1.00
23.41
A
C


ATOM
3741
O
ASN
A
454
39.613
−19.269
19.531
1.00
23.44
A
O


ATOM
3742
N
SER
A
455
40.980
−19.535
17.795
1.00
20.52
A
N


ATOM
3743
CA
SER
A
455
40.342
−20.775
17.361
1.00
19.85
A
C


ATOM
3744
CB
SER
A
455
41.030
−21.305
16.083
1.00
20.87
A
C


ATOM
3745
OG
SER
A
455
40.892
−20.344
15.030
1.00
24.90
A
O


ATOM
3746
C
SER
A
455
40.315
−21.867
18.445
1.00
23.31
A
C


ATOM
3747
O
SER
A
455
39.291
−22.553
18.629
1.00
21.73
A
O


ATOM
3748
N
VAL
A
456
41.436
−22.042
19.143
1.00
19.77
A
N


ATOM
3749
CA
VAL
A
456
41.538
−23.023
20.247
1.00
20.62
A
C


ATOM
3750
CB
VAL
A
456
42.961
−23.046
20.861
1.00
26.15
A
C


ATOM
3751
CG1
VAL
A
456
43.031
−24.075
21.993
1.00
24.79
A
C


ATOM
3752
CG2
VAL
A
456
44.013
−23.297
19.785
1.00
22.76
A
C


ATOM
3753
C
VAL
A
456
40.577
−22.721
21.398
1.00
22.08
A
C


ATOM
3754
O
VAL
A
456
39.960
−23.635
21.969
1.00
22.97
A
O


ATOM
3755
N
SER
A
457
40.440
−21.447
21.756
1.00
21.17
A
N


ATOM
3756
CA
SER
A
457
39.540
−21.119
22.863
1.00
27.43
A
C


ATOM
3757
CB
SER
A
457
39.903
−19.775
23.537
1.00
25.66
A
C


ATOM
3758
OG
SER
A
457
39.705
−18.689
22.677
1.00
36.62
A
O


ATOM
3759
C
SER
A
457
38.079
−21.195
22.410
1.00
25.99
A
C


ATOM
3760
O
SER
A
457
37.211
−21.576
23.198
1.00
26.64
A
O


ATOM
3761
N
CYS
A
458
37.784
−20.907
21.139
1.00
23.94
A
N


ATOM
3762
CA
CYS
A
458
36.407
−21.151
20.649
1.00
21.80
A
C


ATOM
3763
CB
CYS
A
458
36.109
−20.521
19.299
1.00
21.16
A
C


ATOM
3764
SG
CYS
A
458
35.996
−18.776
19.430
1.00
32.42
A
S


ATOM
3765
C
CYS
A
458
36.070
−22.644
20.613
1.00
23.65
A
C


ATOM
3766
O
CYS
A
458
34.923
−23.023
20.854
1.00
24.55
A
O


ATOM
3767
N
TYR
A
459
37.058
−23.483
20.347
1.00
20.39
A
N


ATOM
3768
CA
TYR
A
459
36.814
−24.922
20.314
1.00
24.45
A
C


ATOM
3769
CB
TYR
A
459
37.990
−25.655
19.702
1.00
23.46
A
C


ATOM
3770
CG
TYR
A
459
37.667
−27.042
19.198
1.00
28.55
A
C


ATOM
3771
CD1
TYR
A
459
37.784
−28.153
20.037
1.00
27.38
A
C


ATOM
3772
CE1
TYR
A
459
37.503
−29.441
19.585
1.00
31.49
A
C


ATOM
3773
CZ
TYR
A
459
37.096
−29.635
18.266
1.00
31.60
A
C


ATOM
3774
OH
TYR
A
459
36.813
−30.900
17.824
1.00
37.28
A
O


ATOM
3775
CE2
TYR
A
459
36.969
−28.555
17.407
1.00
26.89
A
C


ATOM
3776
CD2
TYR
A
459
37.255
−27.251
17.882
1.00
32.62
A
C


ATOM
3777
C
TYR
A
459
36.541
−25.427
21.738
1.00
23.84
A
C


ATOM
3778
O
TYR
A
459
35.589
−26.171
21.966
1.00
21.78
A
O


ATOM
3779
N
MET
A
460
37.359
−24.978
22.691
1.00
24.24
A
N


ATOM
3780
CA
MET
A
460
37.134
−25.253
24.120
1.00
23.73
A
C


ATOM
3781
CB
MET
A
460
38.093
−24.432
24.979
1.00
24.01
A
C


ATOM
3782
CG
MET
A
460
39.520
−24.918
24.935
1.00
28.42
A
C


ATOM
3783
SD
MET
A
460
40.629
−23.759
25.730
1.00
34.44
A
S


ATOM
3784
CE
MET
A
460
40.012
−23.901
27.446
1.00
27.52
A
C


ATOM
3785
C
MET
A
460
35.699
−24.952
24.575
1.00
24.85
A
C


ATOM
3786
O
MET
A
460
35.056
−25.765
25.259
1.00
26.64
A
O


ATOM
3787
N
ARG
A
461
35.218
−23.775
24.188
1.00
26.93
A
N


ATOM
3788
CA
ARG
A
461
33.922
−23.276
24.596
1.00
26.56
A
C


ATOM
3789
CB
ARG
A
461
33.789
−21.798
24.236
1.00
25.65
A
C


ATOM
3790
CG
ARG
A
461
32.444
−21.163
24.577
1.00
34.73
A
C


ATOM
3791
CD
ARG
A
461
32.528
−19.649
24.477
1.00
36.40
A
C


ATOM
3792
NE
ARG
A
461
32.808
−19.191
23.111
1.00
34.87
A
N


ATOM
3793
CZ
ARG
A
461
33.253
−17.977
22.788
1.00
36.31
A
C


ATOM
3794
NH1
ARG
A
461
33.494
−17.073
23.740
1.00
37.11
A
N


ATOM
3795
NH2
ARG
A
461
33.461
−17.654
21.504
1.00
35.17
A
N


ATOM
3796
C
ARG
A
461
32.809
−24.077
23.943
1.00
29.33
A
C


ATOM
3797
O
ARG
A
461
31.876
−24.458
24.610
1.00
26.80
A
O


ATOM
3798
N
THR
A
462
32.908
−24.340
22.645
1.00
25.41
A
N


ATOM
3799
CA
THR
A
462
31.825
−25.025
21.931
1.00
25.70
A
C


ATOM
3800
CB
THR
A
462
31.999
−24.938
20.395
1.00
28.39
A
C


ATOM
3801
OG1
THR
A
462
31.983
−23.565
19.979
1.00
19.95
A
O


ATOM
3802
CG2
THR
A
462
30.869
−25.735
19.679
1.00
28.51
A
C


ATOM
3803
C
THR
A
462
31.712
−26.501
22.310
1.00
26.92
A
C


ATOM
3804
O
THR
A
462
30.617
−27.028
22.429
1.00
25.53
A
O


ATOM
3805
N
LYS
A
463
32.847
−27.168
22.474
1.00
31.10
A
N


ATOM
3806
CA
LYS
A
463
32.851
−28.580
22.838
1.00
33.80
A
C


ATOM
3807
CB
LYS
A
463
34.072
−29.287
22.264
1.00
35.18
A
C


ATOM
3808
CG
LYS
A
463
34.167
−29.257
20.764
1.00
41.17
A
C


ATOM
3809
CD
LYS
A
463
32.996
−29.915
20.082
1.00
45.55
A
C


ATOM
3810
CE
LYS
A
463
33.373
−30.271
18.648
1.00
45.12
A
C


ATOM
3811
NZ
LYS
A
463
32.209
−30.829
17.923
1.00
43.29
A
N


ATOM
3812
C
LYS
A
463
32.839
−28.775
24.348
1.00
34.33
A
C


ATOM
3813
O
LYS
A
463
32.583
−29.881
24.811
1.00
34.14
A
O


ATOM
3814
N
GLY
A
464
33.129
−27.716
25.103
1.00
33.67
A
N


ATOM
3815
CA
GLY
A
464
33.148
−27.780
26.570
1.00
37.10
A
C


ATOM
3816
C
GLY
A
464
34.308
−28.622
27.070
1.00
33.81
A
C


ATOM
3817
O
GLY
A
464
34.111
−29.505
27.883
1.00
28.67
A
O


ATOM
3818
N
ILE
A
465
35.519
−28.366
26.579
1.00
26.69
A
N


ATOM
3819
CA
ILE
A
465
36.658
−29.192
26.955
1.00
22.86
A
C


ATOM
3820
CB
ILE
A
465
37.034
−30.183
25.818
1.00
25.64
A
C


ATOM
3821
CG1
ILE
A
465
37.358
−29.446
24.511
1.00
24.83
A
C


ATOM
3822
CD1
ILE
A
465
37.700
−30.355
23.334
1.00
22.74
A
C


ATOM
3823
CG2
ILE
A
465
35.893
−31.178
25.581
1.00
29.07
A
C


ATOM
3824
C
ILE
A
465
37.860
−28.354
27.338
1.00
24.61
A
C


ATOM
3825
O
ILE
A
465
37.915
−27.143
27.073
1.00
25.73
A
O


ATOM
3826
N
SER
A
466
38.845
−29.020
27.923
1.00
24.33
A
N


ATOM
3827
CA
SER
A
466
40.073
−28.378
28.353
1.00
28.81
A
C


ATOM
3828
CB
SER
A
466
40.935
−29.366
29.140
1.00
28.18
A
C


ATOM
3829
OG
SER
A
466
41.359
−30.428
28.295
1.00
25.24
A
O


ATOM
3830
C
SER
A
466
40.891
−27.888
27.177
1.00
28.41
A
C


ATOM
3831
O
SER
A
466
40.745
−28.391
26.052
1.00
30.75
A
O


ATOM
3832
N
GLU
A
467
41.775
−26.933
27.460
1.00
27.64
A
N


ATOM
3833
CA
GLU
A
467
42.778
−26.491
26.518
1.00
33.68
A
C


ATOM
3834
CB
GLU
A
467
43.713
−25.437
27.142
1.00
32.28
A
C


ATOM
3835
CG
GLU
A
467
44.726
−24.910
26.108
1.00
36.53
A
C


ATOM
3836
CD
GLU
A
467
45.627
−23.809
26.617
1.00
45.60
A
C


ATOM
3837
OE1
GLU
A
467
45.418
−23.326
27.751
1.00
44.86
A
O


ATOM
3838
OE2
GLU
A
467
46.555
−23.424
25.865
1.00
43.13
A
O


ATOM
3839
C
GLU
A
467
43.601
−27.655
25.933
1.00
36.62
A
C


ATOM
3840
O
GLU
A
467
43.874
−27.683
24.732
1.00
28.59
A
O


ATOM
3841
N
GLU
A
468
43.979
−28.617
26.766
1.00
28.32
A
N


ATOM
3842
CA
GLU
A
468
44.820
−29.694
26.301
1.00
33.91
A
C


ATOM
3843
CB
GLU
A
468
45.284
−30.563
27.465
1.00
41.97
A
C


ATOM
3844
CG
GLU
A
468
46.394
−31.522
27.070
1.00
58.39
A
C


ATOM
3845
CD
GLU
A
468
47.010
−32.247
28.251
1.00
72.95
A
C


ATOM
3846
OE1
GLU
A
468
46.592
−31.996
29.404
1.00
83.55
A
O


ATOM
3847
OE2
GLU
A
468
47.920
−33.073
28.016
1.00
76.95
A
O


ATOM
3848
C
GLU
A
468
44.106
−30.548
25.237
1.00
31.82
A
C


ATOM
3849
O
GLU
A
468
44.697
−30.877
24.209
1.00
29.90
A
O


ATOM
3850
N
LEU
A
469
42.850
−30.900
25.496
1.00
31.05
A
N


ATOM
3851
CA
LEU
A
469
42.065
−31.697
24.551
1.00
30.37
A
C


ATOM
3852
CB
LEU
A
469
40.771
−32.189
25.190
1.00
34.71
A
C


ATOM
3853
CG
LEU
A
469
40.867
−33.374
26.156
1.00
46.07
A
C


ATOM
3854
CD1
LEU
A
469
39.488
−33.690
26.782
1.00
35.79
A
C


ATOM
3855
CD2
LEU
A
469
41.438
−34.600
25.441
1.00
39.75
A
C


ATOM
3856
C
LEU
A
469
41.722
−30.888
23.289
1.00
29.53
A
C


ATOM
3857
O
LEU
A
469
41.668
−31.440
22.211
1.00
28.42
A
O


ATOM
3858
N
ALA
A
470
41.467
−29.592
23.442
1.00
25.77
A
N


ATOM
3859
CA
ALA
A
470
41.116
−28.735
22.309
1.00
28.38
A
C


ATOM
3860
CB
ALA
A
470
40.642
−27.366
22.767
1.00
21.67
A
C


ATOM
3861
C
ALA
A
470
42.328
−28.626
21.406
1.00
28.76
A
C


ATOM
3862
O
ALA
A
470
42.199
−28.735
20.194
1.00
27.45
A
O


ATOM
3863
N
THR
A
471
43.506
−28.473
22.010
1.00
23.17
A
N


ATOM
3864
CA
THR
A
471
44.751
−28.407
21.284
1.00
24.26
A
C


ATOM
3865
CB
THR
A
471
45.886
−28.155
22.259
1.00
24.13
A
C


ATOM
3866
OG1
THR
A
471
45.651
−26.890
22.876
1.00
25.52
A
O


ATOM
3867
CG2
THR
A
471
47.229
−28.118
21.572
1.00
24.56
A
C


ATOM
3868
C
THR
A
471
45.009
−29.668
20.445
1.00
29.77
A
C


ATOM
3869
O
THR
A
471
45.344
−29.588
19.253
1.00
22.06
A
O


ATOM
3870
N
GLU
A
472
44.855
−30.824
21.064
1.00
22.52
A
N


ATOM
3871
CA
GLU
A
472
44.944
−32.083
20.369
1.00
27.11
A
C


ATOM
3872
CB
GLU
A
472
44.727
−33.204
21.387
1.00
30.22
A
C


ATOM
3873
CG
GLU
A
472
44.802
−34.608
20.851
1.00
38.12
A
C


ATOM
3874
CD
GLU
A
472
44.752
−35.666
21.965
1.00
53.74
A
C


ATOM
3875
OE1
GLU
A
472
44.133
−35.428
23.039
1.00
54.00
A
O


ATOM
3876
OE2
GLU
A
472
45.342
−36.745
21.754
1.00
63.63
A
O


ATOM
3877
C
GLU
A
472
43.938
−32.192
19.198
1.00
25.58
A
C


ATOM
3878
O
GLU
A
472
44.285
−32.658
18.105
1.00
24.92
A
O


ATOM
3879
N
SER
A
473
42.705
−31.767
19.414
1.00
20.39
A
N


ATOM
3880
CA
SER
A
473
41.719
−31.768
18.333
1.00
25.72
A
C


ATOM
3881
CB
SER
A
473
40.355
−31.332
18.814
1.00
26.44
A
C


ATOM
3882
OG
SER
A
473
39.941
−32.144
19.881
1.00
34.79
A
O


ATOM
3883
C
SER
A
473
42.141
−30.835
17.195
1.00
19.41
A
C


ATOM
3884
O
SER
A
473
41.921
−31.148
16.044
1.00
17.90
A
O


ATOM
3885
N
VAL
A
474
42.720
−29.698
17.516
1.00
19.66
A
N


ATOM
3886
CA
VAL
A
474
43.154
−28.780
16.494
1.00
19.80
A
C


ATOM
3887
CB
VAL
A
474
43.533
−27.425
17.074
1.00
21.08
A
C


ATOM
3888
CG1
VAL
A
474
44.344
−26.594
16.023
1.00
19.39
A
C


ATOM
3889
CG2
VAL
A
474
42.271
−26.706
17.477
1.00
22.82
A
C


ATOM
3890
C
VAL
A
474
44.290
−29.380
15.656
1.00
22.88
A
C


ATOM
3891
O
VAL
A
474
44.303
−29.227
14.422
1.00
22.10
A
O


ATOM
3892
N
MET
A
475
45.210
−30.066
16.330
1.00
23.45
A
N


ATOM
3893
CA
MET
A
475
46.330
−30.754
15.683
1.00
26.71
A
C


ATOM
3894
CB
MET
A
475
47.260
−31.391
16.730
1.00
30.53
A
C


ATOM
3895
CG
MET
A
475
47.905
−30.421
17.692
1.00
36.44
A
C


ATOM
3896
SD
MET
A
475
49.127
−29.516
16.796
1.00
41.54
A
S


ATOM
3897
CE
MET
A
475
50.421
−30.764
16.673
1.00
51.84
A
C


ATOM
3898
C
MET
A
475
45.818
−31.839
14.759
1.00
23.38
A
C


ATOM
3899
O
MET
A
475
46.339
−32.029
13.659
1.00
25.85
A
O


ATOM
3900
N
ASN
A
476
44.817
−32.571
15.219
1.00
21.43
A
N


ATOM
3901
CA
ASN
A
476
44.176
−33.555
14.373
1.00
25.46
A
C


ATOM
3902
CB
ASN
A
476
43.201
−34.431
15.160
1.00
28.65
A
C


ATOM
3903
CG
ASN
A
476
43.891
−35.297
16.218
1.00
31.36
A
C


ATOM
3904
OD1
ASN
A
476
45.102
−35.492
16.204
1.00
38.00
A
O


ATOM
3905
ND2
ASN
A
476
43.097
−35.826
17.141
1.00
27.91
A
N


ATOM
3906
C
ASN
A
476
43.475
−32.919
13.176
1.00
21.71
A
C


ATOM
3907
O
ASN
A
476
43.482
−33.507
12.094
1.00
19.35
A
O


ATOM
3908
N
LEU
A
477
42.887
−31.731
13.351
1.00
20.67
A
N


ATOM
3909
CA
ALEU
A
477
42.311
−30.995
12.215
0.50
19.61
A
C


ATOM
3910
CA
BLEU
A
477
42.309
−30.983
12.221
0.50
22.54
A
C


ATOM
3911
CB
ALEU
A
477
41.581
−29.741
12.677
0.50
18.09
A
C


ATOM
3912
CB
BLEU
A
477
41.574
−29.721
12.691
0.50
25.47
A
C


ATOM
3913
CG
ALEU
A
477
40.866
−28.940
11.586
0.50
18.16
A
C


ATOM
3914
CG
BLEU
A
477
40.038
−29.768
12.688
0.50
33.95
A
C


ATOM
3915
CD1
ALEU
A
477
40.121
−29.860
10.614
0.50
21.10
A
C


ATOM
3916
CD1
BLEU
A
477
39.458
−28.519
13.367
0.50
29.79
A
C


ATOM
3917
CD2
ALEU
A
477
39.912
−27.901
12.226
0.50
16.24
A
C


ATOM
3918
CD2
BLEU
A
477
39.506
−29.905
11.257
0.50
35.02
A
C


ATOM
3919
C
LEU
A
477
43.396
−30.616
11.194
1.00
21.48
A
C


ATOM
3920
O
LEU
A
477
43.184
−30.706
9.980
1.00
18.70
A
O


ATOM
3921
N
ILE
A
478
44.553
−30.224
11.684
1.00
17.10
A
N


ATOM
3922
CA
ILE
A
478
45.705
−29.917
10.800
1.00
16.49
A
C


ATOM
3923
CB
ILE
A
478
46.905
−29.359
11.577
1.00
16.79
A
C


ATOM
3924
CG1
ILE
A
478
46.568
−27.911
12.029
1.00
18.33
A
C


ATOM
3925
CD1
ILE
A
478
47.611
−27.299
13.009
1.00
22.73
A
C


ATOM
3926
CG2
ILE
A
478
48.224
−29.287
10.695
1.00
12.76
A
C


ATOM
3927
C
ILE
A
478
46.117
−31.151
10.005
1.00
19.00
A
C


ATOM
3928
O
ILE
A
478
46.283
−31.068
8.781
1.00
16.79
A
O


ATOM
3929
N
ASP
A
479
46.235
−32.294
10.679
1.00
15.78
A
N


ATOM
3930
CA
ASP
A
479
46.574
−33.527
9.978
1.00
17.49
A
C


ATOM
3931
CB
ASP
A
479
46.714
−34.691
10.947
1.00
20.17
A
C


ATOM
3932
CG
ASP
A
479
47.988
−34.598
11.800
1.00
33.33
A
C


ATOM
3933
OD1
ASP
A
479
48.979
−33.880
11.465
1.00
37.08
A
O


ATOM
3934
OD2
ASP
A
479
47.986
−35.279
12.834
1.00
31.99
A
O


ATOM
3935
C
ASP
A
479
45.568
−33.909
8.907
1.00
16.09
A
C


ATOM
3936
O
ASP
A
479
45.956
−34.308
7.800
1.00
16.06
A
O


ATOM
3937
N
GLU
A
480
44.288
−33.873
9.265
1.00
17.62
A
N


ATOM
3938
CA
GLU
A
480
43.212
−34.154
8.308
1.00
19.20
A
C


ATOM
3939
CB
GLU
A
480
41.862
−34.092
9.028
1.00
22.65
A
C


ATOM
3940
CG
GLU
A
480
40.652
−34.191
8.172
1.00
37.11
A
C


ATOM
3941
CD
GLU
A
480
39.410
−33.735
8.938
1.00
52.61
A
C


ATOM
3942
OE1
GLU
A
480
39.183
−34.254
10.046
1.00
53.08
A
O


ATOM
3943
OE2
GLU
A
480
38.689
−32.839
8.451
1.00
51.93
A
O


ATOM
3944
C
GLU
A
480
43.227
−33.238
7.101
1.00
16.53
A
C


ATOM
3945
O
GLU
A
480
43.033
−33.694
5.970
1.00
14.41
A
O


ATOM
3946
N
THR
A
481
43.461
−31.947
7.340
1.00
15.80
A
N


ATOM
3947
CA
THR
A
481
43.569
−30.981
6.290
1.00
13.89
A
C


ATOM
3948
CB
THR
A
481
43.657
−29.560
6.890
1.00
17.77
A
C


ATOM
3949
OG1
THR
A
481
42.509
−29.362
7.753
1.00
19.40
A
O


ATOM
3950
CG2
THR
A
481
43.759
−28.502
5.761
1.00
16.74
A
C


ATOM
3951
C
THR
A
481
44.753
−31.287
5.358
1.00
16.12
A
C


ATOM
3952
O
THR
A
481
44.613
−31.231
4.136
1.00
13.98
A
O


ATOM
3953
N
TRP
A
482
45.912
−31.626
5.919
1.00
15.23
A
N


ATOM
3954
CA
TRP
A
482
47.020
−32.110
5.088
1.00
14.38
A
C


ATOM
3955
CB
TRP
A
482
48.225
−32.441
5.967
1.00
15.52
A
C


ATOM
3956
CG
TRP
A
482
49.119
−31.248
6.112
1.00
16.43
A
C


ATOM
3957
CD1
TRP
A
482
49.158
−30.313
7.151
1.00
17.00
A
C


ATOM
3958
NE1
TRP
A
482
50.107
−29.366
6.895
1.00
18.88
A
N


ATOM
3959
CE2
TRP
A
482
50.679
−29.572
5.689
1.00
16.63
A
C


ATOM
3960
CD2
TRP
A
482
50.076
−30.767
5.126
1.00
13.73
A
C


ATOM
3961
CE3
TRP
A
482
50.490
−31.211
3.881
1.00
18.76
A
C


ATOM
3962
CZ3
TRP
A
482
51.471
−30.491
3.201
1.00
20.35
A
C


ATOM
3963
CH2
TRP
A
482
52.048
−29.341
3.769
1.00
15.16
A
C


ATOM
3964
CZ2
TRP
A
482
51.645
−28.849
5.004
1.00
17.19
A
C


ATOM
3965
C
TRP
A
482
46.663
−33.309
4.206
1.00
15.56
A
C


ATOM
3966
O
TRP
A
482
47.020
−33.322
3.043
1.00
14.64
A
O


ATOM
3967
N
LYS
A
483
45.952
−34.292
4.738
1.00
12.09
A
N


ATOM
3968
CA
LYS
A
483
45.562
−35.442
3.931
1.00
14.07
A
C


ATOM
3969
CB
LYS
A
483
44.834
−36.468
4.751
1.00
12.95
A
C


ATOM
3970
CG
LYS
A
483
45.695
−37.214
5.710
1.00
15.23
A
C


ATOM
3971
CD
LYS
A
483
44.778
−38.101
6.628
1.00
16.87
A
C


ATOM
3972
CE
LYS
A
483
45.546
−38.727
7.744
1.00
20.92
A
C


ATOM
3973
NZ
LYS
A
483
44.621
−39.452
8.640
1.00
19.13
A
N


ATOM
3974
C
LYS
A
483
44.679
−35.024
2.743
1.00
13.93
A
C


ATOM
3975
O
LYS
A
483
44.848
−35.547
1.644
1.00
13.27
A
O


ATOM
3976
N
LYS
A
484
43.810
−34.043
2.959
1.00
14.49
A
N


ATOM
3977
CA
LYS
A
484
42.973
−33.514
1.868
1.00
16.83
A
C


ATOM
3978
CB
LYS
A
484
41.829
−32.688
2.407
1.00
15.87
A
C


ATOM
3979
CG
LYS
A
484
40.884
−33.521
3.224
1.00
19.52
A
C


ATOM
3980
CD
LYS
A
484
39.799
−32.667
3.808
1.00
28.94
A
C


ATOM
3981
CE
LYS
A
484
38.915
−33.469
4.724
1.00
33.21
A
C


ATOM
3982
NZ
LYS
A
484
37.962
−32.556
5.364
1.00
37.29
A
N


ATOM
3983
C
LYS
A
484
43.764
−32.752
0.835
1.00
15.35
A
C


ATOM
3984
O
LYS
A
484
43.563
−32.937
−0.367
1.00
16.38
A
O


ATOM
3985
N
MET
A
485
44.729
−31.963
1.274
1.00
15.56
A
N


ATOM
3986
CA
MET
A
485
45.564
−31.230
0.338
1.00
18.45
A
C


ATOM
3987
CB
MET
A
485
46.475
−30.224
1.047
1.00
17.39
A
C


ATOM
3988
CG
MET
A
485
45.772
−29.041
1.663
1.00
21.79
A
C


ATOM
3989
SD
MET
A
485
46.944
−27.785
2.264
1.00
23.85
A
S


ATOM
3990
CE
MET
A
485
47.995
−28.677
3.419
1.00
21.57
A
C


ATOM
3991
C
MET
A
485
46.434
−32.213
−0.445
1.00
17.61
A
C


ATOM
3992
O
MET
A
485
46.626
−32.015
−1.645
1.00
17.08
A
O


ATOM
3993
N
ASN
A
486
46.885
−33.299
0.199
1.00
18.25
A
N


ATOM
3994
CA
ASN
A
486
47.701
−34.305
−0.469
1.00
15.51
A
C


ATOM
3995
CB
ASN
A
486
48.192
−35.399
0.497
1.00
16.05
A
C


ATOM
3996
CG
ASN
A
486
49.299
−34.941
1.463
1.00
15.97
A
C


ATOM
3997
OD1
ASN
A
486
49.975
−33.950
1.250
1.00
17.54
A
O


ATOM
3998
ND2
ASN
A
486
49.468
−35.706
2.557
1.00
13.12
A
N


ATOM
3999
C
ASN
A
486
46.912
−34.966
−1.621
1.00
17.24
A
C


ATOM
4000
O
ASN
A
486
47.464
−35.234
−2.698
1.00
18.56
A
O


ATOM
4001
N
LYS
A
487
45.635
−35.236
−1.388
1.00
14.64
A
N


ATOM
4002
CA
LYS
A
487
44.782
−35.818
−2.419
1.00
15.66
A
C


ATOM
4003
CB
LYS
A
487
43.422
−36.234
−1.867
1.00
16.91
A
C


ATOM
4004
CG
LYS
A
487
42.572
−36.969
−2.854
1.00
20.96
A
C


ATOM
4005
CD
LYS
A
487
41.260
−37.370
−2.247
1.00
25.53
A
C


ATOM
4006
CE
LYS
A
487
40.344
−37.998
−3.327
1.00
30.21
A
C


ATOM
4007
NZ
LYS
A
487
39.154
−38.633
−2.706
1.00
25.05
A
N


ATOM
4008
C
LYS
A
487
44.591
−34.861
−3.579
1.00
17.27
A
C


ATOM
4009
O
LYS
A
487
44.624
−35.272
−4.753
1.00
17.38
A
O


ATOM
4010
N
GLU
A
488
44.425
−33.593
−3.264
1.00
18.99
A
N


ATOM
4011
CA
GLU
A
488
44.215
−32.556
−4.296
1.00
22.07
A
C


ATOM
4012
CB
GLU
A
488
43.954
−31.191
−3.649
1.00
26.40
A
C


ATOM
4013
CG
GLU
A
488
43.568
−30.048
−4.605
1.00
37.28
A
C


ATOM
4014
CD
GLU
A
488
42.097
−30.103
−5.022
1.00
48.74
A
C


ATOM
4015
OE1
GLU
A
488
41.260
−30.584
−4.221
1.00
54.85
A
O


ATOM
4016
OE2
GLU
A
488
41.783
−29.666
−6.155
1.00
50.63
A
O


ATOM
4017
C
GLU
A
488
45.434
−32.435
−5.209
1.00
19.80
A
C


ATOM
4018
O
GLU
A
488
45.281
−32.265
−6.408
1.00
21.01
A
O


ATOM
4019
N
LYS
A
489
46.630
−32.486
−4.640
1.00
17.29
A
N


ATOM
4020
CA
LYS
A
489
47.851
−32.400
−5.422
1.00
18.72
A
C


ATOM
4021
CB
LYS
A
489
49.063
−32.222
−4.520
1.00
20.27
A
C


ATOM
4022
CG
LYS
A
489
50.438
−32.185
−5.212
1.00
20.51
A
C


ATOM
4023
CD
LYS
A
489
50.631
−30.930
−6.069
1.00
23.34
A
C


ATOM
4024
CE
LYS
A
489
51.862
−31.036
−6.979
1.00
23.63
A
C


ATOM
4025
NZ
LYS
A
489
53.084
−31.210
−6.198
1.00
21.67
A
N


ATOM
4026
C
LYS
A
489
48.039
−33.629
−6.331
1.00
23.93
A
C


ATOM
4027
O
LYS
A
489
48.576
−33.512
−7.443
1.00
21.37
A
O


ATOM
4028
N
LEU
A
490
47.647
−34.796
−5.828
1.00
22.66
A
N


ATOM
4029
CA
ALEU
A
490
47.865
−36.062
−6.550
0.50
27.19
A
C


ATOM
4030
CA
BLEU
A
490
47.823
−36.076
−6.542
0.50
27.96
A
C


ATOM
4031
CB
ALEU
A
490
47.834
−37.243
−5.558
0.50
22.47
A
C


ATOM
4032
CB
BLEU
A
490
47.607
−37.256
−5.594
0.50
24.24
A
C


ATOM
4033
CG
ALEU
A
490
48.080
−38.692
−6.004
0.50
21.71
A
C


ATOM
4034
CG
BLEU
A
490
48.829
−37.831
−4.913
0.50
23.81
A
C


ATOM
4035
CD1
ALEU
A
490
49.436
−38.888
−6.644
0.50
19.67
A
C


ATOM
4036
CD1
BLEU
A
490
48.403
−38.959
−3.950
0.50
21.79
A
C


ATOM
4037
CD2
ALEU
A
490
47.951
−39.598
−4.798
0.50
21.41
A
C


ATOM
4038
CD2
BLEU
A
490
49.805
−38.359
−5.955
0.50
25.26
A
C


ATOM
4039
C
LEU
A
490
46.848
−36.248
−7.682
1.00
30.53
A
C


ATOM
4040
O
LEU
A
490
47.218
−36.645
−8.763
1.00
32.66
A
O


ATOM
4041
N
GLY
A
491
45.590
−35.927
−7.409
1.00
31.34
A
N


ATOM
4042
CA
GLY
A
491
44.478
−36.293
−8.226
1.00
48.80
A
C


ATOM
4043
C
GLY
A
491
44.065
−35.322
−9.283
1.00
60.28
A
C


ATOM
4044
O
GLY
A
491
44.860
−34.939
−10.135
1.00
79.14
A
O


ATOM
4045
N
GLY
A
492
42.796
−34.962
−9.245
1.00
55.84
A
N


ATOM
4046
CA
GLY
A
492
42.162
−34.344
−10.379
1.00
72.32
A
C


ATOM
4047
C
GLY
A
492
41.713
−32.969
−9.983
1.00
72.34
A
C


ATOM
4048
O
GLY
A
492
40.510
−32.700
−9.894
1.00
67.55
A
O


ATOM
4049
N
SER
A
493
42.671
−32.096
−9.721
1.00
53.00
A
N


ATOM
4050
CA
SER
A
493
42.298
−30.727
−9.363
1.00
59.50
A
C


ATOM
4051
CB
SER
A
493
43.453
−29.918
−8.737
1.00
47.61
A
C


ATOM
4052
OG
SER
A
493
42.991
−28.645
−8.187
1.00
43.51
A
O


ATOM
4053
C
SER
A
493
41.800
−30.003
−10.604
1.00
44.76
A
C


ATOM
4054
O
SER
A
493
42.211
−30.287
−11.727
1.00
34.61
A
O


ATOM
4055
N
LEU
A
494
40.917
−29.055
−10.362
1.00
41.66
A
N


ATOM
4056
CA
LEU
A
494
40.589
−28.025
−11.325
1.00
42.41
A
C


ATOM
4057
CB
LEU
A
494
39.490
−27.156
−10.730
1.00
40.97
A
C


ATOM
4058
CG
LEU
A
494
39.214
−25.832
−11.437
1.00
60.70
A
C


ATOM
4059
CD1
LEU
A
494
38.647
−26.045
−12.862
1.00
62.77
A
C


ATOM
4060
CD2
LEU
A
494
38.276
−25.030
−10.568
1.00
49.79
A
C


ATOM
4061
C
LEU
A
494
41.827
−27.172
−11.689
1.00
37.14
A
C


ATOM
4062
O
LEU
A
494
41.975
−26.729
−12.810
1.00
41.80
A
O


ATOM
4063
N
PHE
A
495
42.733
−26.965
−10.743
1.00
26.68
A
N


ATOM
4064
CA
PHE
A
495
43.867
−26.105
−10.990
1.00
26.09
A
C


ATOM
4065
CB
PHE
A
495
44.190
−25.371
−9.706
1.00
23.48
A
C


ATOM
4066
CG
PHE
A
495
43.181
−24.338
−9.344
1.00
25.42
A
C


ATOM
4067
CD1
PHE
A
495
43.301
−23.059
−9.816
1.00
26.17
A
C


ATOM
4068
CE1
PHE
A
495
42.353
−22.089
−9.484
1.00
25.96
A
C


ATOM
4069
CZ
PHE
A
495
41.269
−22.428
−8.689
1.00
27.04
A
C


ATOM
4070
CE2
PHE
A
495
41.139
−23.719
−8.235
1.00
29.85
A
C


ATOM
4071
CD2
PHE
A
495
42.085
−24.664
−8.560
1.00
27.59
A
C


ATOM
4072
C
PHE
A
495
45.085
−26.910
−11.471
1.00
25.94
A
C


ATOM
4073
O
PHE
A
495
45.203
−28.077
−11.170
1.00
28.09
A
O


ATOM
4074
N
ALA
A
496
46.009
−26.247
−12.138
1.00
33.05
A
N


ATOM
4075
CA
ALA
A
496
47.250
−26.868
−12.577
1.00
39.94
A
C


ATOM
4076
CB
ALA
A
496
47.945
−25.966
−13.574
1.00
42.98
A
C


ATOM
4077
C
ALA
A
496
48.150
−27.120
−11.352
1.00
36.62
A
C


ATOM
4078
O
ALA
A
496
48.121
−26.349
−10.389
1.00
31.86
A
O


ATOM
4079
N
LYS
A
497
48.963
−28.167
−11.419
1.00
34.08
A
N


ATOM
4080
CA
LYS
A
497
49.732
−28.637
−10.272
1.00
37.33
A
C


ATOM
4081
CB
LYS
A
497
50.470
−29.961
−10.588
1.00
41.75
A
C


ATOM
4082
CG
LYS
A
497
49.512
−31.161
−10.633
1.00
47.85
A
C


ATOM
4083
CD
LYS
A
497
50.233
−32.512
−10.638
1.00
56.61
A
C


ATOM
4084
CE
LYS
A
497
49.212
−33.653
−10.765
1.00
64.54
A
C


ATOM
4085
NZ
LYS
A
497
49.773
−35.007
−10.458
1.00
65.22
A
N


ATOM
4086
C
LYS
A
497
50.672
−27.606
−9.670
1.00
31.87
A
C


ATOM
4087
O
LYS
A
497
50.822
−27.580
−8.463
1.00
27.56
A
O


ATOM
4088
N
PRO
A
498
51.264
−26.716
−10.492
1.00
33.97
A
N


ATOM
4089
CA
PRO
A
498
52.093
−25.671
−9.909
1.00
32.61
A
C


ATOM
4090
CB
PRO
A
498
52.631
−24.912
−11.132
1.00
34.93
A
C


ATOM
4091
CG
PRO
A
498
52.536
−25.882
−12.254
1.00
42.21
A
C


ATOM
4092
CD
PRO
A
498
51.324
−26.709
−11.966
1.00
41.65
A
C


ATOM
4093
C
PRO
A
498
51.318
−24.707
−9.011
1.00
29.86
A
C


ATOM
4094
O
PRO
A
498
51.840
−24.290
−7.986
1.00
26.30
A
O


ATOM
4095
N
PHE
A
499
50.101
−24.322
−9.389
1.00
26.07
A
N


ATOM
4096
CA
PHE
A
499
49.324
−23.478
−8.486
1.00
25.20
A
C


ATOM
4097
CB
PHE
A
499
48.116
−22.836
−9.142
1.00
21.86
A
C


ATOM
4098
CG
PHE
A
499
47.358
−21.964
−8.214
1.00
20.70
A
C


ATOM
4099
CD1
PHE
A
499
47.912
−20.795
−7.748
1.00
22.74
A
C


ATOM
4100
CE1
PHE
A
499
47.240
−20.000
−6.837
1.00
24.76
A
C


ATOM
4101
CZ
PHE
A
499
45.980
−20.372
−6.418
1.00
22.15
A
C


ATOM
4102
CE2
PHE
A
499
45.434
−21.558
−6.872
1.00
22.89
A
C


ATOM
4103
CD2
PHE
A
499
46.121
−22.328
−7.767
1.00
21.48
A
C


ATOM
4104
C
PHE
A
499
48.901
−24.256
−7.209
1.00
19.68
A
C


ATOM
4105
O
PHE
A
499
48.967
−23.680
−6.103
1.00
20.69
A
O


ATOM
4106
N
VAL
A
500
48.510
−25.536
−7.353
1.00
20.38
A
N


ATOM
4107
CA
VAL
A
500
48.142
−26.363
−6.192
1.00
19.51
A
C


ATOM
4108
CB
VAL
A
500
47.691
−27.750
−6.568
1.00
22.11
A
C


ATOM
4109
CG1
VAL
A
500
47.420
−28.597
−5.314
1.00
20.63
A
C


ATOM
4110
CG2
VAL
A
500
46.418
−27.684
−7.500
1.00
19.82
A
C


ATOM
4111
C
VAL
A
500
49.310
−26.414
−5.237
1.00
17.74
A
C


ATOM
4112
O
VAL
A
500
49.139
−26.210
−4.051
1.00
19.47
A
O


ATOM
4113
N
GLU
A
501
50.518
−26.572
−5.769
1.00
21.36
A
N


ATOM
4114
CA
GLU
A
501
51.722
−26.617
−4.965
1.00
21.86
A
C


ATOM
4115
CB
GLU
A
501
52.903
−27.076
−5.811
1.00
26.57
A
C


ATOM
4116
CG
GLU
A
501
54.218
−27.318
−5.036
1.00
24.39
A
C


ATOM
4117
CD
GLU
A
501
54.178
−28.541
−4.079
1.00
31.48
A
C


ATOM
4118
OE1
GLU
A
501
53.186
−29.319
−4.025
1.00
26.98
A
O


ATOM
4119
OE2
GLU
A
501
55.198
−28.741
−3.395
1.00
27.65
A
O


ATOM
4120
C
GLU
A
501
52.062
−25.283
−4.272
1.00
20.56
A
C


ATOM
4121
O
GLU
A
501
52.447
−25.272
−3.078
1.00
16.73
A
O


ATOM
4122
N
THR
A
502
51.945
−24.178
−4.998
1.00
20.57
A
N


ATOM
4123
CA
THR
A
502
52.097
−22.845
−4.413
1.00
19.79
A
C


ATOM
4124
CB
THR
A
502
51.803
−21.761
−5.462
1.00
24.29
A
C


ATOM
4125
OG1
THR
A
502
52.690
−21.934
−6.551
1.00
25.00
A
O


ATOM
4126
CG2
THR
A
502
51.981
−20.353
−4.890
1.00
22.68
A
C


ATOM
4127
C
THR
A
502
51.146
−22.669
−3.198
1.00
18.49
A
C


ATOM
4128
O
THR
A
502
51.534
−22.190
−2.142
1.00
19.11
A
O


ATOM
4129
N
ALA
A
503
49.914
−23.098
−3.371
1.00
15.97
A
N


ATOM
4130
CA
ALA
A
503
48.885
−23.042
−2.315
1.00
17.68
A
C


ATOM
4131
CB
ALA
A
503
47.546
−23.517
−2.854
1.00
13.94
A
C


ATOM
4132
C
ALA
A
503
49.316
−23.870
−1.123
1.00
18.81
A
C


ATOM
4133
O
ALA
A
503
49.323
−23.384
0.011
1.00
17.14
A
O


ATOM
4134
N
ILE
A
504
49.763
−25.091
−1.373
1.00
20.50
A
N


ATOM
4135
CA
ILE
A
504
50.294
−25.926
−0.282
1.00
18.42
A
C


ATOM
4136
CB
ILE
A
504
50.622
−27.364
−0.791
1.00
19.01
A
C


ATOM
4137
CG1
ILE
A
504
49.347
−28.068
−1.267
1.00
20.65
A
C


ATOM
4138
CD1
ILE
A
504
49.622
−29.508
−1.880
1.00
19.60
A
C


ATOM
4139
CG2
ILE
A
504
51.328
−28.229
0.336
1.00
16.48
A
C


ATOM
4140
C
ILE
A
504
51.523
−25.271
0.360
1.00
20.66
A
C


ATOM
4141
O
ILE
A
504
51.692
−25.327
1.586
1.00
17.24
A
O


ATOM
4142
N
ASN
A
505
52.367
−24.592
−0.423
1.00
18.20
A
N


ATOM
4143
CA
ASN
A
505
53.481
−23.874
0.207
1.00
16.22
A
C


ATOM
4144
CB
ASN
A
505
54.419
−23.250
−0.825
1.00
19.40
A
C


ATOM
4145
CG
ASN
A
505
55.224
−24.278
−1.587
1.00
20.36
A
C


ATOM
4146
OD1
ASN
A
505
55.464
−25.400
−1.111
1.00
19.80
A
O


ATOM
4147
ND2
ASN
A
505
55.697
−23.880
−2.757
1.00
16.17
A
N


ATOM
4148
C
ASN
A
505
53.077
−22.811
1.245
1.00
18.67
A
C


ATOM
4149
O
ASN
A
505
53.839
−22.536
2.172
1.00
19.55
A
O


ATOM
4150
N
LEU
A
506
51.883
−22.250
1.130
1.00
19.40
A
N


ATOM
4151
CA
LEU
A
506
51.415
−21.299
2.133
1.00
18.37
A
C


ATOM
4152
CB
LEU
A
506
50.113
−20.649
1.673
1.00
20.58
A
C


ATOM
4153
CG
LEU
A
506
49.487
−19.672
2.701
1.00
30.53
A
C


ATOM
4154
CD1
LEU
A
506
49.656
−18.279
2.175
1.00
29.62
A
C


ATOM
4155
CD2
LEU
A
506
47.982
−19.947
2.935
1.00
26.29
A
C


ATOM
4156
C
LEU
A
506
51.227
−22.024
3.469
1.00
17.76
A
C


ATOM
4157
O
LEU
A
506
51.540
−21.481
4.551
1.00
16.64
A
O


ATOM
4158
N
ALA
A
507
50.753
−23.271
3.422
1.00
17.78
A
N


ATOM
4159
CA
ALA
A
507
50.632
−24.062
4.636
1.00
18.31
A
C


ATOM
4160
CB
ALA
A
507
49.809
−25.373
4.398
1.00
16.33
A
C


ATOM
4161
C
ALA
A
507
52.016
−24.374
5.220
1.00
17.60
A
C


ATOM
4162
O
ALA
A
507
52.224
−24.289
6.430
1.00
18.28
A
O


ATOM
4163
N
ARG
A
508
52.962
−24.758
4.373
1.00
14.94
A
N


ATOM
4164
CA
ARG
A
508
54.331
−25.035
4.816
1.00
15.68
A
C


ATOM
4165
CB
ARG
A
508
55.193
−25.438
3.624
1.00
15.05
A
C


ATOM
4166
CG
ARG
A
508
54.765
−26.752
2.994
1.00
15.52
A
C


ATOM
4167
CD
ARG
A
508
55.742
−27.210
1.937
1.00
17.52
A
C


ATOM
4168
NE
ARG
A
508
55.436
−28.586
1.540
1.00
18.72
A
N


ATOM
4169
CZ
ARG
A
508
55.023
−28.998
0.347
1.00
20.99
A
C


ATOM
4170
NH1
ARG
A
508
54.826
−28.175
−0.666
1.00
20.60
A
N


ATOM
4171
NH2
ARG
A
508
54.796
−30.287
0.167
1.00
19.00
A
N


ATOM
4172
C
ARG
A
508
54.947
−23.817
5.531
1.00
17.54
A
C


ATOM
4173
O
ARG
A
508
55.547
−23.955
6.610
1.00
19.20
A
O


ATOM
4174
N
GLN
A
509
54.763
−22.640
4.922
1.00
18.14
A
N


ATOM
4175
CA
GLN
A
509
55.322
−21.402
5.475
1.00
18.23
A
C


ATOM
4176
CB
GLN
A
509
55.141
−20.227
4.487
1.00
22.05
A
C


ATOM
4177
CG
GLN
A
509
55.731
−18.902
5.006
1.00
22.47
A
C


ATOM
4178
CD
GLN
A
509
57.226
−18.986
5.232
1.00
27.68
A
C


ATOM
4179
OE1
GLN
A
509
57.930
−19.557
4.446
1.00
26.27
A
O


ATOM
4180
NE2
GLN
A
509
57.699
−18.403
6.302
1.00
34.33
A
N


ATOM
4181
C
GLN
A
509
54.662
−21.115
6.802
1.00
19.66
A
C


ATOM
4182
O
GLN
A
509
55.298
−20.639
7.723
1.00
18.58
A
O


ATOM
4183
N
SER
A
510
53.376
−21.435
6.935
1.00
19.88
A
N


ATOM
4184
CA
SER
A
510
52.698
−21.304
8.224
1.00
16.74
A
C


ATOM
4185
CB
SER
A
510
51.214
−21.683
8.074
1.00
20.01
A
C


ATOM
4186
OG
SER
A
510
50.580
−20.747
7.203
1.00
21.40
A
O


ATOM
4187
C
SER
A
510
53.356
−22.117
9.312
1.00
18.45
A
C


ATOM
4188
O
SER
A
510
53.611
−21.625
10.436
1.00
20.32
A
O


ATOM
4189
N
HIS
A
511
53.617
−23.388
9.021
1.00
16.45
A
N


ATOM
4190
CA
HIS
A
511
54.270
−24.277
9.988
1.00
16.17
A
C


ATOM
4191
CB
HIS
A
511
54.446
−25.675
9.395
1.00
18.95
A
C


ATOM
4192
CG
HIS
A
511
53.170
−26.485
9.362
1.00
16.31
A
C


ATOM
4193
ND1
HIS
A
511
52.624
−27.021
10.495
1.00
20.09
A
N


ATOM
4194
CE1
HIS
A
511
51.483
−27.682
10.169
1.00
22.64
A
C


ATOM
4195
NE2
HIS
A
511
51.314
−27.611
8.847
1.00
18.17
A
N


ATOM
4196
CD2
HIS
A
511
52.341
−26.877
8.305
1.00
20.21
A
C


ATOM
4197
C
HIS
A
511
55.625
−23.734
10.319
1.00
20.90
A
C


ATOM
4198
O
HIS
A
511
56.060
−23.820
11.444
1.00
19.36
A
O


ATOM
4199
N
CYS
A
512
56.303
−23.147
9.340
1.00
19.93
A
N


ATOM
4200
CA
CYS
A
512
57.658
−22.668
9.560
1.00
20.84
A
C


ATOM
4201
CB
CYS
A
512
58.434
−22.617
8.239
1.00
21.43
A
C


ATOM
4202
SG
CYS
A
512
58.833
−24.343
7.612
1.00
26.02
A
S


ATOM
4203
C
CYS
A
512
57.742
−21.291
10.272
1.00
21.58
A
C


ATOM
4204
O
CYS
A
512
58.748
−20.991
10.842
1.00
26.11
A
O


ATOM
4205
N
THR
A
513
56.703
−20.481
10.179
1.00
23.47
A
N


ATOM
4206
CA
THR
A
513
56.676
−19.162
10.788
1.00
27.58
A
C


ATOM
4207
CB
THR
A
513
55.758
−18.254
10.019
1.00
29.85
A
C


ATOM
4208
OG1
THR
A
513
56.247
−18.183
8.682
1.00
27.20
A
O


ATOM
4209
CG2
THR
A
513
55.692
−16.840
10.630
1.00
30.28
A
C


ATOM
4210
C
THR
A
513
56.197
−19.254
12.215
1.00
37.01
A
C


ATOM
4211
O
THR
A
513
56.735
−18.556
13.068
1.00
27.92
A
O


ATOM
4212
N
TYR
A
514
55.216
−20.124
12.484
1.00
27.85
A
N


ATOM
4213
CA
TYR
A
514
54.604
−20.210
13.819
1.00
32.83
A
C


ATOM
4214
CB
TYR
A
514
53.062
−20.286
13.738
1.00
26.86
A
C


ATOM
4215
CG
TYR
A
514
52.532
−19.034
13.105
1.00
22.85
A
C


ATOM
4216
CD1
TYR
A
514
52.511
−17.835
13.795
1.00
31.96
A
C


ATOM
4217
CE1
TYR
A
514
52.084
−16.673
13.177
1.00
30.33
A
C


ATOM
4218
CZ
TYR
A
514
51.698
−16.708
11.868
1.00
31.28
A
C


ATOM
4219
OH
TYR
A
514
51.286
−15.590
11.216
1.00
31.35
A
O


ATOM
4220
CE2
TYR
A
514
51.730
−17.875
11.170
1.00
26.60
A
C


ATOM
4221
CD2
TYR
A
514
52.160
−19.017
11.786
1.00
23.31
A
C


ATOM
4222
C
TYR
A
514
55.242
−21.324
14.605
1.00
37.07
A
C


ATOM
4223
O
TYR
A
514
54.763
−22.428
14.644
1.00
41.12
A
O


ATOM
4224
N
HIS
A
515
56.369
−20.998
15.220
1.00
52.59
A
N


ATOM
4225
CA
HIS
A
515
57.199
−21.961
15.943
1.00
47.40
A
C


ATOM
4226
CB
HIS
A
515
58.533
−22.132
15.211
1.00
47.12
A
C


ATOM
4227
CG
HIS
A
515
59.339
−20.849
15.076
1.00
44.49
A
C


ATOM
4228
ND1
HIS
A
515
59.938
−20.250
16.125
1.00
48.30
A
N


ATOM
4229
CE1
HIS
A
515
60.575
−19.133
15.700
1.00
37.04
A
C


ATOM
4230
NE2
HIS
A
515
60.397
−19.027
14.376
1.00
44.22
A
N


ATOM
4231
CD2
HIS
A
515
59.641
−20.068
13.957
1.00
42.54
A
C


ATOM
4232
C
HIS
A
515
57.428
−21.458
17.335
1.00
60.34
A
C


ATOM
4233
O
HIS
A
515
56.873
−20.424
17.726
1.00
60.05
A
O


ATOM
4234
N
ASN
A
516
58.253
−22.176
18.098
1.00
73.61
A
N


ATOM
4235
CA
ASN
A
516
58.653
−21.727
19.429
1.00
78.32
A
C


ATOM
4236
CB
ASN
A
516
57.754
−22.402
20.469
1.00
76.01
A
C


ATOM
4237
CG
ASN
A
516
57.642
−21.597
21.738
1.00
80.70
A
C


ATOM
4238
OD1
ASN
A
516
57.469
−20.379
21.694
1.00
92.46
A
O


ATOM
4239
ND2
ASN
A
516
57.753
−22.266
22.879
1.00
69.09
A
N


ATOM
4240
C
ASN
A
516
60.146
−21.938
19.790
1.00
71.29
A
C


ATOM
4241
O
ASN
A
516
60.498
−21.929
20.967
1.00
72.15
A
O


ATOM
4242
N
GLY
A
517
61.015
−22.113
18.790
1.00
73.55
A
N


ATOM
4243
CA
GLY
A
517
62.453
−22.324
19.023
1.00
69.30
A
C


ATOM
4244
C
GLY
A
517
62.882
−23.776
18.899
1.00
67.94
A
C


ATOM
4245
O
GLY
A
517
63.971
−24.159
19.346
1.00
52.43
A
O


ATOM
4246
N
THR
A
521
67.697
−22.083
21.593
1.00
48.19
A
N


ATOM
4247
CA
THR
A
521
67.278
−20.802
21.017
1.00
61.81
A
C


ATOM
4248
CB
THR
A
521
67.383
−20.783
19.459
1.00
70.39
A
C


ATOM
4249
OG1
THR
A
521
66.575
−21.828
18.907
1.00
93.82
A
O


ATOM
4250
CG2
THR
A
521
68.823
−20.966
18.990
1.00
66.62
A
C


ATOM
4251
C
THR
A
521
65.840
−20.448
21.409
1.00
61.14
A
C


ATOM
4252
O
THR
A
521
65.037
−21.330
21.732
1.00
64.90
A
O


ATOM
4253
N
SER
A
522
65.535
−19.150
21.372
1.00
54.62
A
N


ATOM
4254
CA
SER
A
522
64.209
−18.614
21.715
1.00
58.24
A
C


ATOM
4255
CB
SER
A
522
64.388
−17.390
22.594
1.00
53.59
A
C


ATOM
4256
OG
SER
A
522
64.988
−16.352
21.835
1.00
46.59
A
O


ATOM
4257
C
SER
A
522
63.452
−18.213
20.435
1.00
54.40
A
C


ATOM
4258
O
SER
A
522
64.060
−18.147
19.365
1.00
52.75
A
O


ATOM
4259
N
PRO
A
523
62.136
−17.931
20.537
1.00
59.75
A
N


ATOM
4260
CA
PRO
A
523
61.363
−17.599
19.329
1.00
67.72
A
C


ATOM
4261
CB
PRO
A
523
60.025
−17.100
19.895
1.00
67.43
A
C


ATOM
4262
CG
PRO
A
523
59.858
−17.915
21.128
1.00
61.02
A
C


ATOM
4263
CD
PRO
A
523
61.251
−18.079
21.710
1.00
60.09
A
C


ATOM
4264
C
PRO
A
523
62.000
−16.576
18.375
1.00
63.23
A
C


ATOM
4265
O
PRO
A
523
62.426
−16.965
17.289
1.00
65.32
A
O


ATOM
4266
N
ASP
A
524
62.098
−15.301
18.751
1.00
53.76
A
N


ATOM
4267
CA
ASP
A
524
62.463
−14.292
17.746
1.00
56.64
A
C


ATOM
4268
CB
ASP
A
524
61.997
−12.888
18.126
1.00
72.74
A
C


ATOM
4269
CG
ASP
A
524
61.249
−12.221
16.980
1.00
90.60
A
C


ATOM
4270
OD1
ASP
A
524
61.879
−11.892
15.952
1.00
92.92
A
O


ATOM
4271
OD2
ASP
A
524
60.017
−12.076
17.088
1.00
99.57
A
O


ATOM
4272
C
ASP
A
524
63.938
−14.318
17.345
1.00
43.11
A
C


ATOM
4273
O
ASP
A
524
64.314
−13.682
16.370
1.00
45.57
A
O


ATOM
4274
N
GLU
A
525
64.758
−15.053
18.093
1.00
38.74
A
N


ATOM
4275
CA
GLU
A
525
66.127
−15.348
17.683
1.00
47.99
A
C


ATOM
4276
CB
GLU
A
525
66.966
−15.818
18.879
1.00
57.18
A
C


ATOM
4277
CG
GLU
A
525
68.471
−15.653
18.703
1.00
76.26
A
C


ATOM
4278
CD
GLU
A
525
68.893
−14.230
18.331
1.00
86.79
A
C


ATOM
4279
OE1
GLU
A
525
68.255
−13.251
18.780
1.00
83.59
A
O


ATOM
4280
OE2
GLU
A
525
69.877
−14.096
17.577
1.00
87.42
A
O


ATOM
4281
C
GLU
A
525
66.144
−16.417
16.569
1.00
38.28
A
C


ATOM
4282
O
GLU
A
525
66.952
−16.332
15.656
1.00
42.11
A
O


ATOM
4283
N
LEU
A
526
65.269
−17.414
16.656
1.00
42.35
A
N


ATOM
4284
CA
LEU
A
526
65.125
−18.397
15.570
1.00
41.74
A
C


ATOM
4285
CB
LEU
A
526
64.186
−19.549
15.974
1.00
41.93
A
C


ATOM
4286
CG
LEU
A
526
64.128
−20.773
15.034
1.00
47.17
A
C


ATOM
4287
CD1
LEU
A
526
65.540
−21.285
14.673
1.00
39.29
A
C


ATOM
4288
CD2
LEU
A
526
63.265
−21.912
15.626
1.00
42.53
A
C


ATOM
4289
C
LEU
A
526
64.658
−17.701
14.282
1.00
37.23
A
C


ATOM
4290
O
LEU
A
526
65.289
−17.850
13.223
1.00
38.03
A
O


ATOM
4291
N
THR
A
527
63.588
−16.915
14.376
1.00
34.27
A
N


ATOM
4292
CA
THR
A
527
63.107
−16.129
13.231
1.00
35.49
A
C


ATOM
4293
CB
THR
A
527
61.935
−15.222
13.639
1.00
37.44
A
C


ATOM
4294
OG1
THR
A
527
60.916
−16.040
14.216
1.00
37.80
A
O


ATOM
4295
CG2
THR
A
527
61.357
−14.444
12.439
1.00
30.44
A
C


ATOM
4296
C
THR
A
527
64.200
−15.275
12.611
1.00
46.48
A
C


ATOM
4297
O
THR
A
527
64.328
−15.218
11.388
1.00
46.01
A
O


ATOM
4298
N
ARG
A
528
64.996
−14.617
13.452
1.00
41.37
A
N


ATOM
4299
CA
ARG
A
528
66.030
−13.727
12.944
1.00
40.07
A
C


ATOM
4300
CB
ARG
A
528
66.596
−12.873
14.074
1.00
49.72
A
C


ATOM
4301
CG
ARG
A
528
67.347
−11.637
13.603
1.00
68.35
A
C


ATOM
4302
CD
ARG
A
528
68.111
−10.980
14.753
1.00
72.05
A
C


ATOM
4303
NE
ARG
A
528
69.010
−11.933
15.411
1.00
84.73
A
N


ATOM
4304
CZ
ARG
A
528
70.157
−12.384
14.896
1.00
84.45
A
C


ATOM
4305
NH1
ARG
A
528
70.581
−11.975
13.702
1.00
92.83
A
N


ATOM
4306
NH2
ARG
A
528
70.890
−13.258
15.579
1.00
68.97
A
N


ATOM
4307
C
ARG
A
528
67.150
−14.510
12.254
1.00
37.77
A
C


ATOM
4308
O
ARG
A
528
67.717
−14.045
11.265
1.00
36.26
A
O


ATOM
4309
N
LYS
A
529
67.502
−15.670
12.800
1.00
32.17
A
N


ATOM
4310
CA
LYS
A
529
68.492
−16.536
12.148
1.00
43.23
A
C


ATOM
4311
CB
LYS
A
529
68.929
−17.676
13.068
1.00
44.04
A
C


ATOM
4312
CG
LYS
A
529
69.925
−17.250
14.143
1.00
61.21
A
C


ATOM
4313
CD
LYS
A
529
70.050
−18.326
15.206
1.00
65.83
A
C


ATOM
4314
CE
LYS
A
529
70.831
−17.849
16.414
1.00
83.82
A
C


ATOM
4315
NZ
LYS
A
529
70.540
−18.727
17.589
1.00
91.28
A
N


ATOM
4316
C
LYS
A
529
67.955
−17.097
10.808
1.00
31.54
A
C


ATOM
4317
O
LYS
A
529
68.703
−17.225
9.845
1.00
42.10
A
O


ATOM
4318
N
ARG
A
530
66.668
−17.399
10.748
1.00
36.46
A
N


ATOM
4319
CA
ARG
A
530
66.092
−17.952
9.515
1.00
37.01
A
C


ATOM
4320
CB
ARG
A
530
64.741
−18.562
9.786
1.00
36.05
A
C


ATOM
4321
CG
ARG
A
530
64.872
−19.834
10.584
1.00
32.99
A
C


ATOM
4322
CD
ARG
A
530
63.544
−20.448
10.839
1.00
35.75
A
C


ATOM
4323
NE
ARG
A
530
63.709
−21.788
11.389
1.00
34.23
A
N


ATOM
4324
CZ
ARG
A
530
62.714
−22.560
11.804
1.00
33.65
A
C


ATOM
4325
NH1
ARG
A
530
61.455
−22.164
11.696
1.00
35.22
A
N


ATOM
4326
NH2
ARG
A
530
62.987
−23.750
12.325
1.00
40.16
A
N


ATOM
4327
C
ARG
A
530
66.048
−16.897
8.425
1.00
40.32
A
C


ATOM
4328
O
ARG
A
530
66.475
−17.148
7.291
1.00
32.17
A
O


ATOM
4329
N
VAL
A
531
65.612
−15.697
8.796
1.00
33.10
A
N


ATOM
4330
CA
VAL
A
531
65.576
−14.584
7.866
1.00
35.68
A
C


ATOM
4331
CB
VAL
A
531
64.874
−13.355
8.497
1.00
42.31
A
C


ATOM
4332
CG1
VAL
A
531
64.901
−12.166
7.547
1.00
41.62
A
C


ATOM
4333
CG2
VAL
A
531
63.425
−13.716
8.878
1.00
37.84
A
C


ATOM
4334
C
VAL
A
531
66.979
−14.256
7.357
1.00
41.35
A
C


ATOM
4335
O
VAL
A
531
67.170
−13.964
6.172
1.00
46.12
A
O


ATOM
4336
N
LEU
A
532
67.970
−14.325
8.234
1.00
40.42
A
N


ATOM
4337
CA
LEU
A
532
69.344
−14.093
7.811
1.00
39.71
A
C


ATOM
4338
CB
LEU
A
532
70.300
−14.000
9.004
1.00
43.42
A
C


ATOM
4339
CG
LEU
A
532
70.692
−12.579
9.478
1.00
53.48
A
C


ATOM
4340
CD1
LEU
A
532
69.496
−11.619
9.718
1.00
47.39
A
C


ATOM
4341
CD2
LEU
A
532
71.538
−12.720
10.726
1.00
45.63
A
C


ATOM
4342
C
LEU
A
532
69.837
−15.180
6.838
1.00
46.33
A
C


ATOM
4343
O
LEU
A
532
70.445
−14.862
5.825
1.00
44.36
A
O


ATOM
4344
N
SER
A
533
69.576
−16.454
7.141
1.00
35.58
A
N


ATOM
4345
CA
SER
A
533
70.105
−17.555
6.316
1.00
34.63
A
C


ATOM
4346
CB
SER
A
533
70.076
−18.850
7.112
1.00
31.52
A
C


ATOM
4347
OG
SER
A
533
68.746
−19.297
7.268
1.00
33.57
A
O


ATOM
4348
C
SER
A
533
69.358
−17.712
4.982
1.00
25.58
A
C


ATOM
4349
O
SER
A
533
69.916
−18.225
4.001
1.00
27.36
A
O


ATOM
4350
N
VAL
A
534
68.113
−17.254
4.925
1.00
26.19
A
N


ATOM
4351
CA
VAL
A
534
67.324
−17.302
3.667
1.00
23.87
A
C


ATOM
4352
CB
VAL
A
534
65.824
−17.504
3.957
1.00
24.85
A
C


ATOM
4353
CG1
VAL
A
534
64.962
−17.401
2.688
1.00
19.21
A
C


ATOM
4354
CG2
VAL
A
534
65.589
−18.861
4.600
1.00
26.32
A
C


ATOM
4355
C
VAL
A
534
67.525
−16.070
2.765
1.00
26.84
A
C


ATOM
4356
O
VAL
A
534
67.650
−16.194
1.550
1.00
28.14
A
O


ATOM
4357
N
ILE
A
535
67.540
−14.874
3.349
1.00
28.94
A
N


ATOM
4358
CA
ILE
A
535
67.516
−13.635
2.563
1.00
28.39
A
C


ATOM
4359
CB
ILE
A
535
66.538
−12.596
3.171
1.00
34.98
A
C


ATOM
4360
CG1
ILE
A
535
65.113
−13.130
3.251
1.00
35.70
A
C


ATOM
4361
CD1
ILE
A
535
64.479
−13.463
1.911
1.00
44.01
A
C


ATOM
4362
CG2
ILE
A
535
66.582
−11.268
2.372
1.00
35.77
A
C


ATOM
4363
C
ILE
A
535
68.882
−12.956
2.465
1.00
32.00
A
C


ATOM
4364
O
ILE
A
535
69.306
−12.535
1.385
1.00
38.68
A
O


ATOM
4365
N
THR
A
536
69.570
−12.825
3.589
1.00
35.57
A
N


ATOM
4366
CA
THR
A
536
70.650
−11.815
3.680
1.00
35.50
A
C


ATOM
4367
CB
THR
A
536
70.440
−10.922
4.927
1.00
44.42
A
C


ATOM
4368
OG1
THR
A
536
70.630
−11.686
6.120
1.00
46.85
A
O


ATOM
4369
CG2
THR
A
536
69.023
−10.376
4.936
1.00
34.58
A
C


ATOM
4370
C
THR
A
536
72.080
−12.379
3.578
1.00
35.15
A
C


ATOM
4371
O
THR
A
536
72.899
−11.856
2.819
1.00
32.71
A
O


ATOM
4372
N
GLU
A
537
72.375
−13.460
4.287
1.00
31.94
A
N


ATOM
4373
CA
GLU
A
537
73.728
−14.005
4.299
1.00
38.13
A
C


ATOM
4374
CB
GLU
A
537
74.089
−14.472
5.704
1.00
44.29
A
C


ATOM
4375
CG
GLU
A
537
74.061
−13.369
6.740
1.00
56.93
A
C


ATOM
4376
CD
GLU
A
537
74.966
−13.668
7.913
1.00
59.02
A
C


ATOM
4377
OE1
GLU
A
537
75.055
−14.854
8.296
1.00
56.89
A
O


ATOM
4378
OE2
GLU
A
537
75.588
−12.723
8.447
1.00
59.60
A
O


ATOM
4379
C
GLU
A
537
73.884
−15.190
3.346
1.00
32.80
A
C


ATOM
4380
O
GLU
A
537
73.136
−16.127
3.443
1.00
29.57
A
O


ATOM
4381
N
PRO
A
538
74.877
−15.153
2.447
1.00
29.81
A
N


ATOM
4382
CA
PRO
A
538
75.135
−16.339
1.643
1.00
27.02
A
C


ATOM
4383
CB
PRO
A
538
76.189
−15.876
0.625
1.00
28.93
A
C


ATOM
4384
CG
PRO
A
538
76.740
−14.597
1.160
1.00
34.72
A
C


ATOM
4385
CD
PRO
A
538
75.672
−13.990
2.011
1.00
34.41
A
C


ATOM
4386
C
PRO
A
538
75.664
−17.498
2.457
1.00
26.35
A
C


ATOM
4387
O
PRO
A
538
76.199
−17.301
3.526
1.00
20.63
A
O


ATOM
4388
N
ILE
A
539
75.474
−18.709
1.942
1.00
24.14
A
N


ATOM
4389
CA
ILE
A
539
76.119
−19.895
2.469
1.00
22.55
A
C


ATOM
4390
CB
ILE
A
539
75.580
−21.185
1.795
1.00
23.63
A
C


ATOM
4391
CG1
ILE
A
539
74.110
−21.388
2.197
1.00
23.50
A
C


ATOM
4392
CD1
ILE
A
539
73.484
−22.693
1.716
1.00
20.21
A
C


ATOM
4393
CG2
ILE
A
539
76.433
−22.376
2.151
1.00
20.66
A
C


ATOM
4394
C
ILE
A
539
77.603
−19.760
2.176
1.00
24.55
A
C


ATOM
4395
O
ILE
A
539
77.995
−19.240
1.116
1.00
17.55
A
O


ATOM
4396
N
LEU
A
540
78.415
−20.260
3.099
1.00
20.97
A
N


ATOM
4397
CA
LEU
A
540
79.866
−20.080
3.033
1.00
23.53
A
C


ATOM
4398
CB
LEU
A
540
80.554
−20.454
4.361
1.00
25.37
A
C


ATOM
4399
CG
LEU
A
540
80.573
−19.421
5.505
1.00
37.87
A
C


ATOM
4400
CD1
LEU
A
540
81.220
−20.026
6.753
1.00
41.81
A
C


ATOM
4401
CD2
LEU
A
540
81.320
−18.094
5.146
1.00
33.15
A
C


ATOM
4402
C
LEU
A
540
80.381
−20.922
1.888
1.00
24.37
A
C


ATOM
4403
O
LEU
A
540
79.937
−22.063
1.713
1.00
26.94
A
O


ATOM
4404
N
PRO
A
541
81.312
−20.366
1.092
1.00
26.27
A
N


ATOM
4405
CA
PRO
A
541
81.687
−20.986
−0.188
1.00
23.27
A
C


ATOM
4406
CB
PRO
A
541
82.687
−19.997
−0.800
1.00
28.51
A
C


ATOM
4407
CG
PRO
A
541
83.029
−19.016
0.303
1.00
29.70
A
C


ATOM
4408
CD
PRO
A
541
81.901
−19.021
1.266
1.00
29.11
A
C


ATOM
4409
C
PRO
A
541
82.278
−22.374
−0.058
1.00
24.70
A
C


ATOM
4410
O
PRO
A
541
82.858
−22.721
0.971
1.00
25.93
A
O


ATOM
4411
N
PHE
A
542
82.098
−23.185
−1.095
1.00
24.37
A
N


ATOM
4412
CA
PHE
A
542
82.703
−24.513
−1.151
1.00
21.75
A
C


ATOM
4413
CB
PHE
A
542
82.437
−25.081
−2.522
1.00
21.15
A
C


ATOM
4414
CG
PHE
A
542
83.008
−26.416
−2.729
1.00
22.86
A
C


ATOM
4415
CD1
PHE
A
542
82.505
−27.496
−2.037
1.00
23.96
A
C


ATOM
4416
CE1
PHE
A
542
83.011
−28.753
−2.231
1.00
26.40
A
C


ATOM
4417
CZ
PHE
A
542
84.050
−28.953
−3.127
1.00
29.66
A
C


ATOM
4418
CE2
PHE
A
542
84.574
−27.875
−3.826
1.00
28.49
A
C


ATOM
4419
CD2
PHE
A
542
84.048
−26.613
−3.629
1.00
26.57
A
C


ATOM
4420
C
PHE
A
542
84.225
−24.449
−0.980
1.00
25.16
A
C


ATOM
4421
O
PHE
A
542
84.829
−23.566
−1.544
1.00
21.55
A
O


ATOM
4422
N
GLU
A
543
84.827
−25.395
−0.253
1.00
34.39
A
N


ATOM
4423
CA
GLU
A
543
86.302
−25.457
−0.097
1.00
47.54
A
C


ATOM
4424
CB
GLU
A
543
86.720
−25.276
1.373
1.00
57.01
A
C


ATOM
4425
CG
GLU
A
543
86.935
−23.819
1.792
1.00
59.28
A
C


ATOM
4426
CD
GLU
A
543
87.824
−23.691
3.025
1.00
71.22
A
C


ATOM
4427
OE1
GLU
A
543
88.980
−24.175
3.003
1.00
69.03
A
O


ATOM
4428
OE2
GLU
A
543
87.368
−23.092
4.018
1.00
82.75
A
O


ATOM
4429
C
GLU
A
543
86.981
−26.698
−0.714
1.00
50.91
A
C


ATOM
4430
O
GLU
A
543
87.247
−26.713
−1.913
1.00
56.87
A
O


ATOM
4431
N
ARG
A
544
87.287
−27.720
0.085
1.00
67.82
A
N


ATOM
4432
CA
ARG
A
544
88.181
−28.797
−0.371
1.00
70.50
A
C


ATOM
4433
CB
ARG
A
544
89.581
−28.230
−0.696
1.00
76.08
A
C


ATOM
4434
CG
ARG
A
544
90.546
−29.200
−1.391
1.00
90.28
A
C


ATOM
4435
CD
ARG
A
544
89.885
−30.008
−2.515
1.00
101.63
A
C


ATOM
4436
NE
ARG
A
544
89.111
−29.177
−3.442
1.00
99.45
A
N


ATOM
4437
CZ
ARG
A
544
88.374
−29.647
−4.448
1.00
93.65
A
C


ATOM
4438
NH1
ARG
A
544
88.292
−30.950
−4.678
1.00
95.79
A
N


ATOM
4439
NH2
ARG
A
544
87.710
−28.808
−5.235
1.00
91.46
A
N


ATOM
4440
C
ARG
A
544
88.309
−29.910
0.662
1.00
65.35
A
C


ATOM
4441
O
ARG
A
544
89.318
−30.003
1.364
1.00
66.29
A
O


ATOM
4442
N
SER
B
6
66.028
35.448
11.860
1.00
47.07
B
N


ATOM
4443
CA
SER
B
6
66.385
36.705
11.127
1.00
58.24
B
C


ATOM
4444
CB
SER
B
6
66.400
37.899
12.073
1.00
56.45
B
C


ATOM
4445
OG
SER
B
6
66.861
39.069
11.413
1.00
72.79
B
O


ATOM
4446
C
SER
B
6
67.715
36.596
10.348
1.00
60.55
B
C


ATOM
4447
O
SER
B
6
67.735
36.911
9.153
1.00
56.56
B
O


ATOM
4448
N
ALA
B
7
68.816
36.179
11.000
1.00
42.85
B
N


ATOM
4449
CA
ALA
B
7
69.983
35.673
10.240
1.00
35.66
B
C


ATOM
4450
CB
ALA
B
7
71.252
35.554
11.104
1.00
30.55
B
C


ATOM
4451
C
ALA
B
7
69.550
34.315
9.718
1.00
31.48
B
C


ATOM
4452
O
ALA
B
7
68.673
33.684
10.306
1.00
28.34
B
O


ATOM
4453
N
ASN
B
8
70.119
33.860
8.607
1.00
37.62
B
N


ATOM
4454
CA
ASN
B
8
69.707
32.579
8.041
1.00
36.50
B
C


ATOM
4455
CB
ASN
B
8
68.925
32.796
6.739
1.00
40.61
B
C


ATOM
4456
CG
ASN
B
8
68.518
31.498
6.081
1.00
39.92
B
C


ATOM
4457
OD1
ASN
B
8
68.472
30.436
6.712
1.00
47.46
B
O


ATOM
4458
ND2
ASN
B
8
68.215
31.577
4.798
1.00
59.84
B
N


ATOM
4459
C
ASN
B
8
70.866
31.612
7.813
1.00
35.29
B
C


ATOM
4460
O
ASN
B
8
71.528
31.650
6.779
1.00
39.53
B
O


ATOM
4461
N
TYR
B
9
71.066
30.709
8.767
1.00
32.98
B
N


ATOM
4462
CA
TYR
B
9
72.154
29.740
8.692
1.00
31.48
B
C


ATOM
4463
CB
TYR
B
9
72.806
29.570
10.075
1.00
25.69
B
C


ATOM
4464
CG
TYR
B
9
73.331
30.877
10.652
1.00
21.84
B
C


ATOM
4465
CD1
TYR
B
9
74.259
31.663
9.945
1.00
22.23
B
C


ATOM
4466
CE1
TYR
B
9
74.748
32.876
10.495
1.00
18.59
B
C


ATOM
4467
CZ
TYR
B
9
74.268
33.313
11.720
1.00
14.14
B
C


ATOM
4468
OH
TYR
B
9
74.738
34.483
12.270
1.00
15.10
B
O


ATOM
4469
CE2
TYR
B
9
73.328
32.554
12.420
1.00
13.57
B
C


ATOM
4470
CD2
TYR
B
9
72.875
31.350
11.887
1.00
17.61
B
C


ATOM
4471
C
TYR
B
9
71.703
28.390
8.131
1.00
35.04
B
C


ATOM
4472
O
TYR
B
9
72.488
27.448
8.105
1.00
39.68
B
O


ATOM
4473
N
GLU
B
10
70.459
28.296
7.660
1.00
42.28
B
N


ATOM
4474
CA
GLU
B
10
69.933
27.015
7.152
1.00
47.58
B
C


ATOM
4475
CB
GLU
B
10
68.391
26.999
7.200
1.00
51.50
B
C


ATOM
4476
CG
GLU
B
10
67.797
27.312
8.605
1.00
60.77
B
C


ATOM
4477
CD
GLU
B
10
68.474
26.526
9.740
1.00
69.74
B
C


ATOM
4478
OE1
GLU
B
10
68.629
25.294
9.600
1.00
71.24
B
O


ATOM
4479
OE2
GLU
B
10
68.858
27.137
10.766
1.00
81.34
B
O


ATOM
4480
C
GLU
B
10
70.481
26.715
5.741
1.00
38.79
B
C


ATOM
4481
O
GLU
B
10
70.707
27.644
4.952
1.00
35.80
B
O


ATOM
4482
N
PRO
B
11
70.740
25.425
5.435
1.00
48.23
B
N


ATOM
4483
CA
PRO
B
11
71.364
25.091
4.154
1.00
45.24
B
C


ATOM
4484
CB
PRO
B
11
71.760
23.621
4.329
1.00
53.83
B
C


ATOM
4485
CG
PRO
B
11
70.740
23.077
5.276
1.00
58.80
B
C


ATOM
4486
CD
PRO
B
11
70.432
24.214
6.227
1.00
57.54
B
C


ATOM
4487
C
PRO
B
11
70.363
25.244
3.011
1.00
38.09
B
C


ATOM
4488
O
PRO
B
11
69.158
25.173
3.242
1.00
37.80
B
O


ATOM
4489
N
ASN
B
12
70.862
25.500
1.809
1.00
35.42
B
N


ATOM
4490
CA
ASN
B
12
70.033
25.475
0.594
1.00
36.63
B
C


ATOM
4491
CB
ASN
B
12
70.491
26.541
−0.406
1.00
44.07
B
C


ATOM
4492
CG
ASN
B
12
70.595
27.907
0.215
1.00
48.52
B
C


ATOM
4493
OD1
ASN
B
12
69.591
28.482
0.615
1.00
57.64
B
O


ATOM
4494
ND2
ASN
B
12
71.816
28.437
0.311
1.00
44.25
B
N


ATOM
4495
C
ASN
B
12
70.058
24.097
−0.068
1.00
33.77
B
C


ATOM
4496
O
ASN
B
12
71.034
23.324
0.058
1.00
34.32
B
O


ATOM
4497
N
SER
B
13
68.967
23.772
−0.758
1.00
43.14
B
N


ATOM
4498
CA
SER
B
13
68.857
22.493
−1.483
1.00
41.27
B
C


ATOM
4499
CB
SER
B
13
67.454
22.324
−2.091
1.00
35.20
B
C


ATOM
4500
OG
SER
B
13
67.033
23.498
−2.759
1.00
39.06
B
O


ATOM
4501
C
SER
B
13
69.923
22.356
−2.586
1.00
40.57
B
C


ATOM
4502
O
SER
B
13
70.258
21.243
−2.991
1.00
43.94
B
O


ATOM
4503
N
TRP
B
14
70.462
23.489
−3.039
1.00
30.52
B
N


ATOM
4504
CA
TRP
B
14
71.523
23.518
−4.062
1.00
35.59
B
C


ATOM
4505
CB
TRP
B
14
71.226
24.606
−5.107
1.00
32.98
B
C


ATOM
4506
CG
TRP
B
14
70.716
25.912
−4.544
1.00
36.10
B
C


ATOM
4507
CD1
TRP
B
14
69.387
26.315
−4.410
1.00
45.32
B
C


ATOM
4508
NE1
TRP
B
14
69.306
27.561
−3.841
1.00
45.60
B
N


ATOM
4509
CE2
TRP
B
14
70.538
28.040
−3.577
1.00
38.78
B
C


ATOM
4510
CD2
TRP
B
14
71.503
27.017
−4.006
1.00
34.57
B
C


ATOM
4511
CE3
TRP
B
14
72.849
27.256
−3.838
1.00
41.97
B
C


ATOM
4512
CZ3
TRP
B
14
73.250
28.469
−3.262
1.00
55.13
B
C


ATOM
4513
CH2
TRP
B
14
72.317
29.432
−2.863
1.00
53.40
B
C


ATOM
4514
CZ2
TRP
B
14
70.937
29.230
−3.015
1.00
42.74
B
C


ATOM
4515
C
TRP
B
14
72.950
23.665
−3.521
1.00
36.47
B
C


ATOM
4516
O
TRP
B
14
73.909
23.621
−4.304
1.00
28.14
B
O


ATOM
4517
N
ASP
B
15
73.128
23.825
−2.203
1.00
31.81
B
N


ATOM
4518
CA
ASP
B
15
74.491
23.876
−1.611
1.00
28.30
B
C


ATOM
4519
CB
ASP
B
15
74.425
23.877
−0.071
1.00
33.88
B
C


ATOM
4520
CG
ASP
B
15
74.011
25.218
0.518
1.00
40.45
B
C


ATOM
4521
OD1
ASP
B
15
73.764
26.174
−0.234
1.00
42.94
B
O


ATOM
4522
OD2
ASP
B
15
73.930
25.309
1.763
1.00
54.72
B
O


ATOM
4523
C
ASP
B
15
75.319
22.637
−2.029
1.00
33.86
B
C


ATOM
4524
O
ASP
B
15
74.784
21.523
−2.110
1.00
26.74
B
O


ATOM
4525
N
TYR
B
16
76.625
22.819
−2.228
1.00
28.03
B
N


ATOM
4526
CA
TYR
B
16
77.536
21.728
−2.628
1.00
27.83
B
C


ATOM
4527
CB
TYR
B
16
78.917
22.286
−3.056
1.00
25.77
B
C


ATOM
4528
CG
TYR
B
16
78.893
23.078
−4.351
1.00
24.05
B
C


ATOM
4529
CD1
TYR
B
16
78.370
22.517
−5.531
1.00
22.17
B
C


ATOM
4530
CE1
TYR
B
16
78.355
23.234
−6.721
1.00
17.53
B
C


ATOM
4531
CZ
TYR
B
16
78.814
24.517
−6.732
1.00
20.83
B
C


ATOM
4532
OH
TYR
B
16
78.770
25.206
−7.896
1.00
19.17
B
O


ATOM
4533
CE2
TYR
B
16
79.329
25.107
−5.579
1.00
24.55
B
C


ATOM
4534
CD2
TYR
B
16
79.364
24.379
−4.399
1.00
24.85
B
C


ATOM
4535
C
TYR
B
16
77.714
20.649
−1.525
1.00
35.70
B
C


ATOM
4536
O
TYR
B
16
77.945
19.473
−1.835
1.00
32.15
B
O


ATOM
4537
N
ASP
B
17
77.618
21.053
−0.255
1.00
30.24
B
N


ATOM
4538
CA
ASP
B
17
77.597
20.080
0.869
1.00
42.23
B
C


ATOM
4539
CB
ASP
B
17
77.534
20.812
2.219
1.00
38.98
B
C


ATOM
4540
CG
ASP
B
17
78.841
21.499
2.587
1.00
44.68
B
C


ATOM
4541
OD1
ASP
B
17
79.926
20.995
2.204
1.00
44.13
B
O


ATOM
4542
OD2
ASP
B
17
78.770
22.546
3.275
1.00
44.42
B
O


ATOM
4543
C
ASP
B
17
76.400
19.110
0.748
1.00
45.86
B
C


ATOM
4544
O
ASP
B
17
76.523
17.919
1.008
1.00
51.23
B
O


ATOM
4545
N
TYR
B
18
75.259
19.647
0.329
1.00
49.27
B
N


ATOM
4546
CA
TYR
B
18
74.025
18.877
0.133
1.00
50.51
B
C


ATOM
4547
CB
TYR
B
18
72.840
19.861
0.083
1.00
60.94
B
C


ATOM
4548
CG
TYR
B
18
71.471
19.297
0.384
1.00
82.34
B
C


ATOM
4549
CD1
TYR
B
18
70.797
18.508
−0.555
1.00
91.64
B
C


ATOM
4550
CE1
TYR
B
18
69.531
18.007
−0.293
1.00
96.76
B
C


ATOM
4551
CZ
TYR
B
18
68.916
18.307
0.913
1.00
100.88
B
C


ATOM
4552
OH
TYR
B
18
67.662
17.809
1.169
1.00
105.08
B
O


ATOM
4553
CE2
TYR
B
18
69.553
19.103
1.858
1.00
92.37
B
C


ATOM
4554
CD2
TYR
B
18
70.821
19.598
1.586
1.00
87.10
B
C


ATOM
4555
C
TYR
B
18
74.116
18.045
−1.158
1.00
48.99
B
C


ATOM
4556
O
TYR
B
18
73.977
16.828
−1.127
1.00
44.28
B
O


ATOM
4557
N
LEU
B
19
74.383
18.704
−2.287
1.00
43.39
B
N


ATOM
4558
CA
LEU
B
19
74.349
18.061
−3.610
1.00
39.30
B
C


ATOM
4559
CB
LEU
B
19
74.222
19.115
−4.734
1.00
40.10
B
C


ATOM
4560
CG
LEU
B
19
72.909
19.881
−4.924
1.00
36.96
B
C


ATOM
4561
CD1
LEU
B
19
73.106
20.911
−6.037
1.00
33.51
B
C


ATOM
4562
CD2
LEU
B
19
71.702
18.942
−5.242
1.00
35.19
B
C


ATOM
4563
C
LEU
B
19
75.530
17.131
−3.931
1.00
38.74
B
C


ATOM
4564
O
LEU
B
19
75.448
16.358
−4.870
1.00
33.93
B
O


ATOM
4565
N
LEU
B
20
76.626
17.207
−3.184
1.00
43.02
B
N


ATOM
4566
CA
LEU
B
20
77.784
16.337
−3.446
1.00
48.34
B
C


ATOM
4567
CB
LEU
B
20
78.956
17.186
−3.980
1.00
48.91
B
C


ATOM
4568
CG
LEU
B
20
78.737
17.933
−5.311
1.00
42.22
B
C


ATOM
4569
CD1
LEU
B
20
79.941
18.813
−5.681
1.00
32.75
B
C


ATOM
4570
CD2
LEU
B
20
78.427
16.957
−6.428
1.00
41.67
B
C


ATOM
4571
C
LEU
B
20
78.159
15.566
−2.162
1.00
53.90
B
C


ATOM
4572
O
LEU
B
20
78.522
16.183
−1.166
1.00
50.11
B
O


ATOM
4573
N
SER
B
21
78.064
14.229
−2.185
1.00
73.10
B
N


ATOM
4574
CA
SER
B
21
78.141
13.417
−0.947
1.00
87.01
B
C


ATOM
4575
CB
SER
B
21
76.733
13.253
−0.351
1.00
82.80
B
C


ATOM
4576
OG
SER
B
21
76.250
14.465
0.191
1.00
87.39
B
O


ATOM
4577
C
SER
B
21
78.772
12.011
−1.059
1.00
105.74
B
C


ATOM
4578
O
SER
B
21
79.742
11.697
−0.360
1.00
95.58
B
O


ATOM
4579
N
SER
B
22
78.198
11.160
−1.905
1.00
118.22
B
N


ATOM
4580
CA
SER
B
22
78.488
9.719
−1.861
1.00
113.76
B
C


ATOM
4581
CB
SER
B
22
77.376
8.940
−2.574
1.00
109.60
B
C


ATOM
4582
OG
SER
B
22
77.051
9.533
−3.823
1.00
113.06
B
O


ATOM
4583
C
SER
B
22
79.857
9.357
−2.439
1.00
101.67
B
C


ATOM
4584
O
SER
B
22
80.196
9.751
−3.552
1.00
111.02
B
O


ATOM
4585
N
ILE
B
28
83.597
12.639
−5.109
1.00
85.65
B
N


ATOM
4586
CA
ILE
B
28
82.666
13.752
−4.965
1.00
86.60
B
C


ATOM
4587
CB
ILE
B
28
81.164
13.263
−5.008
1.00
93.39
B
C


ATOM
4588
CG1
ILE
B
28
80.923
12.256
−6.149
1.00
83.16
B
C


ATOM
4589
CD1
ILE
B
28
79.442
12.122
−6.580
1.00
61.20
B
C


ATOM
4590
CG2
ILE
B
28
80.211
14.427
−5.205
1.00
98.61
B
C


ATOM
4591
C
ILE
B
28
82.985
14.552
−3.680
1.00
83.50
B
C


ATOM
4592
O
ILE
B
28
82.171
15.365
−3.233
1.00
79.32
B
O


ATOM
4593
N
GLU
B
29
84.180
14.325
−3.117
1.00
76.18
B
N


ATOM
4594
CA
GLU
B
29
84.667
15.025
−1.915
1.00
76.21
B
C


ATOM
4595
CB
GLU
B
29
85.131
14.003
−0.865
1.00
76.32
B
C


ATOM
4596
CG
GLU
B
29
85.858
14.579
0.372
1.00
72.53
B
C


ATOM
4597
CD
GLU
B
29
84.923
15.133
1.438
1.00
70.19
B
C


ATOM
4598
OE1
GLU
B
29
83.782
15.526
1.121
1.00
71.77
B
O


ATOM
4599
OE2
GLU
B
29
85.338
15.175
2.611
1.00
63.15
B
O


ATOM
4600
C
GLU
B
29
85.815
15.997
−2.242
1.00
83.08
B
C


ATOM
4601
O
GLU
B
29
85.858
17.125
−1.734
1.00
68.15
B
O


ATOM
4602
N
VAL
B
30
86.761
15.543
−3.060
1.00
82.68
B
N


ATOM
4603
CA
VAL
B
30
87.825
16.416
−3.557
1.00
72.16
B
C


ATOM
4604
CB
VAL
B
30
88.928
15.620
−4.327
1.00
69.91
B
C


ATOM
4605
CG1
VAL
B
30
88.342
14.879
−5.566
1.00
57.81
B
C


ATOM
4606
CG2
VAL
B
30
90.079
16.544
−4.719
1.00
50.91
B
C


ATOM
4607
C
VAL
B
30
87.217
17.503
−4.454
1.00
59.36
B
C


ATOM
4608
O
VAL
B
30
87.598
18.665
−4.356
1.00
50.25
B
O


ATOM
4609
N
TYR
B
31
86.264
17.096
−5.297
1.00
47.13
B
N


ATOM
4610
CA
TYR
B
31
85.540
17.957
−6.261
1.00
52.78
B
C


ATOM
4611
CB
TYR
B
31
84.753
17.013
−7.199
1.00
53.13
B
C


ATOM
4612
CG
TYR
B
31
83.853
17.527
−8.333
1.00
51.24
B
C


ATOM
4613
CD1
TYR
B
31
84.321
17.610
−9.653
1.00
53.18
B
C


ATOM
4614
CE1
TYR
B
31
83.460
18.012
−10.722
1.00
52.11
B
C


ATOM
4615
CZ
TYR
B
31
82.099
18.293
−10.468
1.00
63.53
B
C


ATOM
4616
OH
TYR
B
31
81.214
18.693
−11.496
1.00
34.19
B
O


ATOM
4617
CE2
TYR
B
31
81.620
18.175
−9.158
1.00
63.84
B
C


ATOM
4618
CD2
TYR
B
31
82.489
17.772
−8.111
1.00
58.51
B
C


ATOM
4619
C
TYR
B
31
84.628
18.966
−5.526
1.00
45.55
B
C


ATOM
4620
O
TYR
B
31
84.466
20.108
−5.978
1.00
32.99
B
O


ATOM
4621
N
LYS
B
32
84.073
18.547
−4.382
1.00
39.91
B
N


ATOM
4622
CA
LYS
B
32
83.222
19.401
−3.535
1.00
37.60
B
C


ATOM
4623
CB
LYS
B
32
82.581
18.578
−2.406
1.00
35.13
B
C


ATOM
4624
CG
LYS
B
32
81.635
19.380
−1.518
1.00
37.69
B
C


ATOM
4625
CD
LYS
B
32
81.148
18.584
−0.328
1.00
46.73
B
C


ATOM
4626
CE
LYS
B
32
82.243
18.417
0.707
1.00
54.45
B
C


ATOM
4627
NZ
LYS
B
32
81.709
18.007
2.029
1.00
58.88
B
N


ATOM
4628
C
LYS
B
32
84.009
20.563
−2.929
1.00
29.15
B
C


ATOM
4629
O
LYS
B
32
83.567
21.704
−2.945
1.00
26.62
B
O


ATOM
4630
N
ASP
B
33
85.171
20.262
−2.380
1.00
26.85
B
N


ATOM
4631
CA
ASP
B
33
86.043
21.304
−1.850
1.00
31.14
B
C


ATOM
4632
CB
ASP
B
33
87.244
20.694
−1.112
1.00
40.70
B
C


ATOM
4633
CG
ASP
B
33
86.860
20.052
0.234
1.00
51.84
B
C


ATOM
4634
OD1
ASP
B
33
85.654
20.001
0.587
1.00
46.52
B
O


ATOM
4635
OD2
ASP
B
33
87.783
19.596
0.943
1.00
66.19
B
O


ATOM
4636
C
ASP
B
33
86.522
22.273
−2.944
1.00
27.80
B
C


ATOM
4637
O
ASP
B
33
86.731
23.460
−2.661
1.00
19.96
B
O


ATOM
4638
N
LYS
B
34
86.701
21.784
−4.179
1.00
28.92
B
N


ATOM
4639
CA
LYS
B
34
87.096
22.666
−5.288
1.00
30.71
B
C


ATOM
4640
CB
LYS
B
34
87.463
21.925
−6.581
1.00
40.79
B
C


ATOM
4641
CG
LYS
B
34
88.951
21.833
−6.793
1.00
62.22
B
C


ATOM
4642
CD
LYS
B
34
89.356
20.754
−7.790
1.00
73.27
B
C


ATOM
4643
CE
LYS
B
34
90.722
20.184
−7.405
1.00
75.22
B
C


ATOM
4644
NZ
LYS
B
34
91.222
19.199
−8.395
1.00
77.49
B
N


ATOM
4645
C
LYS
B
34
85.957
23.595
−5.552
1.00
19.79
B
C


ATOM
4646
O
LYS
B
34
86.165
24.802
−5.613
1.00
21.44
B
O


ATOM
4647
N
ALA
B
35
84.752
23.041
−5.657
1.00
17.70
B
N


ATOM
4648
CA
ALA
B
35
83.578
23.838
−5.959
1.00
17.27
B
C


ATOM
4649
CB
ALA
B
35
82.324
22.968
−6.139
1.00
21.47
B
C


ATOM
4650
C
ALA
B
35
83.346
24.917
−4.902
1.00
14.58
B
C


ATOM
4651
O
ALA
B
35
83.132
26.055
−5.249
1.00
13.40
B
O


ATOM
4652
N
LYS
B
36
83.412
24.566
−3.628
1.00
13.58
B
N


ATOM
4653
CA
LYS
B
36
83.192
25.542
−2.571
1.00
16.14
B
C


ATOM
4654
CB
LYS
B
36
83.192
24.844
−1.209
1.00
22.56
B
C


ATOM
4655
CG
LYS
B
36
81.867
24.120
−0.931
1.00
25.63
B
C


ATOM
4656
CD
LYS
B
36
82.003
22.927
0.008
1.00
27.13
B
C


ATOM
4657
CE
LYS
B
36
82.113
23.335
1.455
1.00
27.92
B
C


ATOM
4658
NZ
LYS
B
36
82.483
22.191
2.365
1.00
34.58
B
N


ATOM
4659
C
LYS
B
36
84.201
26.716
−2.577
1.00
16.08
B
C


ATOM
4660
O
LYS
B
36
83.808
27.852
−2.389
1.00
14.45
B
O


ATOM
4661
N
LYS
B
37
85.490
26.438
−2.789
1.00
16.82
B
N


ATOM
4662
CA
LYS
B
37
86.513
27.496
−2.841
1.00
15.72
B
C


ATOM
4663
CB
LYS
B
37
87.887
26.871
−3.009
1.00
18.50
B
C


ATOM
4664
CG
LYS
B
37
89.029
27.830
−2.842
1.00
24.53
B
C


ATOM
4665
CD
LYS
B
37
90.353
27.059
−2.666
1.00
28.56
B
C


ATOM
4666
CE
LYS
B
37
91.523
27.969
−2.240
1.00
36.91
B
C


ATOM
4667
NZ
LYS
B
37
91.795
29.070
−3.227
1.00
35.36
B
N


ATOM
4668
C
LYS
B
37
86.258
28.476
−4.000
1.00
14.34
B
C


ATOM
4669
O
LYS
B
37
86.366
29.696
−3.831
1.00
14.98
B
O


ATOM
4670
N
LEU
B
38
85.948
27.927
−5.173
1.00
12.95
B
N


ATOM
4671
CA
LEU
B
38
85.584
28.724
−6.356
1.00
13.59
B
C


ATOM
4672
CB
LEU
B
38
85.367
27.815
−7.579
1.00
13.70
B
C


ATOM
4673
CG
LEU
B
38
86.576
26.956
−8.026
1.00
14.80
B
C


ATOM
4674
CD1
LEU
B
38
86.156
25.994
−9.138
1.00
15.35
B
C


ATOM
4675
CD2
LEU
B
38
87.708
27.807
−8.496
1.00
16.16
B
C


ATOM
4676
C
LEU
B
38
84.331
29.580
−6.128
1.00
11.73
B
C


ATOM
4677
O
LEU
B
38
84.312
30.774
−6.434
1.00
10.49
B
O


ATOM
4678
N
GLU
B
39
83.284
28.957
−5.593
1.00
11.44
B
N


ATOM
4679
CA
GLU
B
39
82.039
29.651
−5.285
1.00
10.93
B
C


ATOM
4680
CB
GLU
B
39
81.024
28.641
−4.707
1.00
14.27
B
C


ATOM
4681
CG
GLU
B
39
79.661
29.208
−4.277
1.00
15.52
B
C


ATOM
4682
CD
GLU
B
39
78.760
28.118
−3.648
1.00
21.97
B
C


ATOM
4683
OE1
GLU
B
39
79.308
27.233
−2.932
1.00
20.74
B
O


ATOM
4684
OE2
GLU
B
39
77.519
28.145
−3.871
1.00
19.35
B
O


ATOM
4685
C
GLU
B
39
82.260
30.782
−4.295
1.00
10.22
B
C


ATOM
4686
O
GLU
B
39
81.707
31.874
−4.441
1.00
11.77
B
O


ATOM
4687
N
ALA
B
40
83.049
30.514
−3.263
1.00
11.24
B
N


ATOM
4688
CA
ALA
B
40
83.413
31.549
−2.275
1.00
10.54
B
C


ATOM
4689
CB
ALA
B
40
84.445
30.961
−1.271
1.00
8.92
B
C


ATOM
4690
C
ALA
B
40
83.988
32.820
−2.922
1.00
11.00
B
C


ATOM
4691
O
ALA
B
40
83.643
33.967
−2.573
1.00
10.39
B
O


ATOM
4692
N
GLU
B
41
84.921
32.593
−3.832
1.00
11.84
B
N


ATOM
4693
CA
GLU
B
41
85.642
33.679
−4.482
1.00
13.73
B
C


ATOM
4694
CB
GLU
B
41
86.845
33.130
−5.257
1.00
16.77
B
C


ATOM
4695
CG
GLU
B
41
87.556
34.171
−6.075
1.00
23.70
B
C


ATOM
4696
CD
GLU
B
41
88.983
33.774
−6.425
1.00
26.91
B
C


ATOM
4697
OE1
GLU
B
41
89.536
32.847
−5.786
1.00
28.61
B
O


ATOM
4698
OE2
GLU
B
41
89.524
34.397
−7.361
1.00
28.33
B
O


ATOM
4699
C
GLU
B
41
84.700
34.465
−5.371
1.00
11.19
B
C


ATOM
4700
O
GLU
B
41
84.766
35.677
−5.373
1.00
12.00
B
O


ATOM
4701
N
VAL
B
42
83.789
33.793
−6.078
1.00
11.09
B
N


ATOM
4702
CA
VAL
B
42
82.786
34.501
−6.891
1.00
10.93
B
C


ATOM
4703
CB
VAL
B
42
81.997
33.525
−7.747
1.00
9.55
B
C


ATOM
4704
CG1
VAL
B
42
80.857
34.255
−8.496
1.00
9.17
B
C


ATOM
4705
CG2
VAL
B
42
82.928
32.811
−8.738
1.00
8.08
B
C


ATOM
4706
C
VAL
B
42
81.839
35.323
−5.990
1.00
12.84
B
C


ATOM
4707
O
VAL
B
42
81.520
36.490
−6.260
1.00
8.45
B
O


ATOM
4708
N
ARG
B
43
81.461
34.738
−4.855
1.00
13.35
B
N


ATOM
4709
CA
ARG
B
43
80.627
35.466
−3.894
1.00
12.68
B
C


ATOM
4710
CB
ARG
B
43
80.256
34.564
−2.678
1.00
16.05
B
C


ATOM
4711
CG
ARG
B
43
79.596
35.299
−1.520
1.00
15.98
B
C


ATOM
4712
CD
ARG
B
43
80.551
36.025
−0.555
1.00
14.84
B
C


ATOM
4713
NE
ARG
B
43
79.792
36.738
0.477
1.00
12.83
B
N


ATOM
4714
CZ
ARG
B
43
80.287
37.581
1.367
1.00
13.48
B
C


ATOM
4715
NH1
ARG
B
43
81.604
37.873
1.419
1.00
12.76
B
N


ATOM
4716
NH2
ARG
B
43
79.450
38.143
2.236
1.00
13.52
B
N


ATOM
4717
C
ARG
B
43
81.333
36.719
−3.455
1.00
10.00
B
C


ATOM
4718
O
ARG
B
43
80.724
37.800
−3.453
1.00
9.91
B
O


ATOM
4719
N
ARG
B
44
82.622
36.610
−3.095
1.00
10.02
B
N


ATOM
4720
CA
ARG
B
44
83.403
37.766
−2.649
1.00
11.29
B
C


ATOM
4721
CB
ARG
B
44
84.859
37.387
−2.317
1.00
11.05
B
C


ATOM
4722
CG
ARG
B
44
85.738
38.573
−1.939
1.00
12.30
B
C


ATOM
4723
CD
ARG
B
44
87.177
38.170
−1.662
1.00
13.93
B
C


ATOM
4724
NE
ARG
B
44
87.816
37.720
−2.879
1.00
13.54
B
N


ATOM
4725
CZ
ARG
B
44
88.955
37.037
−2.934
1.00
15.26
B
C


ATOM
4726
NH1
ARG
B
44
89.639
36.695
−1.820
1.00
15.94
B
N


ATOM
4727
NH2
ARG
B
44
89.434
36.723
−4.136
1.00
17.40
B
N


ATOM
4728
C
ARG
B
44
83.405
38.901
−3.711
1.00
13.01
B
C


ATOM
4729
O
ARG
B
44
83.240
40.084
−3.381
1.00
12.57
B
O


ATOM
4730
N
GLU
B
45
83.640
38.530
−4.957
1.00
12.75
B
N


ATOM
4731
CA
GLU
B
45
83.748
39.520
−6.062
1.00
13.49
B
C


ATOM
4732
CB
GLU
B
45
84.261
38.852
−7.335
1.00
14.55
B
C


ATOM
4733
CG
GLU
B
45
85.693
38.391
−7.210
1.00
20.78
B
C


ATOM
4734
CD
GLU
B
45
86.595
39.480
−6.687
1.00
22.05
B
C


ATOM
4735
OE1
GLU
B
45
86.700
40.507
−7.381
1.00
30.70
B
O


ATOM
4736
OE2
GLU
B
45
87.156
39.348
−5.565
1.00
23.89
B
O


ATOM
4737
C
GLU
B
45
82.411
40.195
−6.356
1.00
11.85
B
C


ATOM
4738
O
GLU
B
45
82.383
41.369
−6.652
1.00
13.11
B
O


ATOM
4739
N
ILE
B
46
81.303
39.476
−6.257
1.00
13.19
B
N


ATOM
4740
CA
ILE
B
46
79.982
40.124
−6.405
1.00
13.57
B
C


ATOM
4741
CB
ILE
B
46
78.837
39.124
−6.541
1.00
13.61
B
C


ATOM
4742
CG1
ILE
B
46
78.999
38.312
−7.834
1.00
14.91
B
C


ATOM
4743
CD1
ILE
B
46
78.232
36.993
−7.820
1.00
15.21
B
C


ATOM
4744
CG2
ILE
B
46
77.406
39.838
−6.519
1.00
13.13
B
C


ATOM
4745
C
ILE
B
46
79.704
41.089
−5.248
1.00
15.53
B
C


ATOM
4746
O
ILE
B
46
79.070
42.114
−5.447
1.00
16.29
B
O


ATOM
4747
N
ASN
B
47
80.211
40.779
−4.055
1.00
14.45
B
N


ATOM
4748
CA
ASN
B
47
79.945
41.573
−2.857
1.00
13.64
B
C


ATOM
4749
CB
ASN
B
47
79.982
40.663
−1.616
1.00
13.48
B
C


ATOM
4750
CG
ASN
B
47
78.682
39.912
−1.409
1.00
15.18
B
C


ATOM
4751
OD1
ASN
B
47
77.783
40.444
−0.809
1.00
19.56
B
O


ATOM
4752
ND2
ASN
B
47
78.562
38.700
−1.935
1.00
14.68
B
N


ATOM
4753
C
ASN
B
47
80.920
42.729
−2.689
1.00
14.30
B
C


ATOM
4754
O
ASN
B
47
80.787
43.524
−1.753
1.00
15.59
B
O


ATOM
4755
N
ASN
B
48
81.935
42.782
−3.553
1.00
12.89
B
N


ATOM
4756
CA
ASN
B
48
83.002
43.732
−3.436
1.00
14.23
B
C


ATOM
4757
CB
ASN
B
48
84.104
43.415
−4.452
1.00
15.10
B
C


ATOM
4758
CG
ASN
B
48
85.190
44.481
−4.524
1.00
14.60
B
C


ATOM
4759
OD1
ASN
B
48
85.436
45.247
−3.582
1.00
14.32
B
O


ATOM
4760
ND2
ASN
B
48
85.876
44.499
−5.640
1.00
14.84
B
N


ATOM
4761
C
ASN
B
48
82.461
45.145
−3.657
1.00
18.92
B
C


ATOM
4762
O
ASN
B
48
82.064
45.520
−4.743
1.00
18.59
B
O


ATOM
4763
N
GLU
B
49
82.526
45.894
−2.579
1.00
16.96
B
N


ATOM
4764
CA
AGLU
B
49
81.891
47.222
−2.595
0.50
20.16
B
C


ATOM
4765
CA
BGLU
B
49
82.012
47.211
−2.332
0.50
20.40
B
C


ATOM
4766
CB
AGLU
B
49
81.283
47.614
−1.246
0.50
20.23
B
C


ATOM
4767
CB
BGLU
B
49
82.092
47.349
−0.763
0.50
20.55
B
C


ATOM
4768
CG
AGLU
B
49
79.871
47.057
−1.095
0.50
18.95
B
C


ATOM
4769
CG
BGLU
B
49
82.748
46.010
0.056
0.50
17.18
B
C


ATOM
4770
CD
AGLU
B
49
79.177
47.488
0.179
0.50
20.35
B
C


ATOM
4771
CD
BGLU
B
49
82.014
45.559
1.354
0.50
20.63
B
C


ATOM
4772
OE1
AGLU
B
49
79.264
48.678
0.538
0.50
18.79
B
O


ATOM
4773
OE1
BGLU
B
49
81.815
44.321
1.607
0.50
12.18
B
O


ATOM
4774
OE2
AGLU
B
49
78.516
46.636
0.804
0.50
20.33
B
O


ATOM
4775
OE2
BGLU
B
49
81.659
46.441
2.165
0.50
35.93
B
O


ATOM
4776
C
GLU
B
49
82.806
48.297
−3.137
1.00
23.82
B
C


ATOM
4777
O
GLU
B
49
82.351
49.413
−3.361
1.00
23.93
B
O


ATOM
4778
N
LYS
B
50
84.041
47.946
−3.496
1.00
23.25
B
N


ATOM
4779
CA
LYS
B
50
84.950
48.858
−4.183
1.00
25.39
B
C


ATOM
4780
CB
LYS
B
50
86.360
48.773
−3.582
1.00
26.32
B
C


ATOM
4781
CG
LYS
B
50
86.467
49.109
−2.104
1.00
30.70
B
C


ATOM
4782
CD
LYS
B
50
87.925
49.077
−1.631
1.00
36.98
B
C


ATOM
4783
CE
LYS
B
50
88.324
47.760
−0.997
1.00
36.26
B
C


ATOM
4784
NZ
LYS
B
50
87.920
47.744
0.432
1.00
37.46
B
N


ATOM
4785
C
LYS
B
50
85.056
48.586
−5.683
1.00
21.74
B
C


ATOM
4786
O
LYS
B
50
85.849
49.221
−6.341
1.00
19.87
B
O


ATOM
4787
N
ALA
B
51
84.298
47.641
−6.228
1.00
20.75
B
N


ATOM
4788
CA
ALA
B
51
84.388
47.339
−7.670
1.00
23.95
B
C


ATOM
4789
CB
ALA
B
51
83.564
46.099
−8.031
1.00
24.01
B
C


ATOM
4790
C
ALA
B
51
83.889
48.533
−8.483
1.00
26.40
B
C


ATOM
4791
O
ALA
B
51
83.035
49.262
−8.019
1.00
19.87
B
O


ATOM
4792
N
GLU
B
52
84.429
48.726
−9.685
1.00
30.89
B
N


ATOM
4793
CA
GLU
B
52
83.891
49.712
−10.619
1.00
32.03
B
C


ATOM
4794
CB
GLU
B
52
84.814
49.918
−11.826
1.00
34.13
B
C


ATOM
4795
CG
GLU
B
52
86.179
50.492
−11.479
1.00
48.84
B
C


ATOM
4796
CD
GLU
B
52
86.105
51.808
−10.703
1.00
55.69
B
C


ATOM
4797
OE1
GLU
B
52
85.026
52.439
−10.662
1.00
59.80
B
O


ATOM
4798
OE2
GLU
B
52
87.135
52.216
−10.129
1.00
73.34
B
O


ATOM
4799
C
GLU
B
52
82.539
49.221
−11.096
1.00
30.95
B
C


ATOM
4800
O
GLU
B
52
82.356
48.028
−11.327
1.00
23.44
B
O


ATOM
4801
N
PHE
B
53
81.596
50.141
−11.252
1.00
30.12
B
N


ATOM
4802
CA
PHE
B
53
80.218
49.742
−11.473
1.00
32.98
B
C


ATOM
4803
CB
PHE
B
53
79.251
50.936
−11.338
1.00
43.11
B
C


ATOM
4804
CG
PHE
B
53
78.867
51.269
−9.878
1.00
55.16
B
C


ATOM
4805
CD1
PHE
B
53
78.505
50.251
−8.961
1.00
64.23
B
C


ATOM
4806
CE1
PHE
B
53
78.139
50.555
−7.624
1.00
59.12
B
C


ATOM
4807
CZ
PHE
B
53
78.126
51.883
−7.199
1.00
55.08
B
C


ATOM
4808
CE2
PHE
B
53
78.477
52.909
−8.101
1.00
60.25
B
C


ATOM
4809
CD2
PHE
B
53
78.843
52.598
−9.429
1.00
60.00
B
C


ATOM
4810
C
PHE
B
53
80.066
48.968
−12.786
1.00
28.16
B
C


ATOM
4811
O
PHE
B
53
79.474
47.890
−12.797
1.00
21.25
B
O


ATOM
4812
N
LEU
B
54
80.665
49.435
−13.868
1.00
23.34
B
N


ATOM
4813
CA
LEU
B
54
80.566
48.668
−15.134
1.00
25.10
B
C


ATOM
4814
CB
LEU
B
54
81.148
49.457
−16.309
1.00
29.41
B
C


ATOM
4815
CG
LEU
B
54
80.706
49.050
−17.729
1.00
44.51
B
C


ATOM
4816
CD1
LEU
B
54
80.836
50.213
−18.709
1.00
41.57
B
C


ATOM
4817
CD2
LEU
B
54
81.510
47.879
−18.233
1.00
45.75
B
C


ATOM
4818
C
LEU
B
54
81.165
47.235
−15.058
1.00
23.44
B
C


ATOM
4819
O
LEU
B
54
80.552
46.288
−15.538
1.00
20.26
B
O


ATOM
4820
N
ATHR
B
55
82.338
47.073
−14.451
0.50
23.03
B
N


ATOM
4821
N
BTHR
B
55
82.340
47.092
−14.444
0.50
21.21
B
N


ATOM
4822
CA
ATHR
B
55
82.943
45.737
−14.351
0.50
22.88
B
C


ATOM
4823
CA
BTHR
B
55
82.990
45.778
−14.298
0.50
19.98
B
C


ATOM
4824
CB
ATHR
B
55
84.383
45.784
−13.810
0.50
24.47
B
C


ATOM
4825
CB
BTHR
B
55
84.402
45.909
−13.662
0.50
19.41
B
C


ATOM
4826
OG1
ATHR
B
55
84.365
46.120
−12.417
0.50
26.50
B
O


ATOM
4827
OG1
BTHR
B
55
85.192
46.839
−14.419
0.50
20.82
B
O


ATOM
4828
CG2
ATHR
B
55
85.214
46.808
−14.582
0.50
26.31
B
C


ATOM
4829
CG2
BTHR
B
55
85.124
44.551
−13.597
0.50
16.45
B
C


ATOM
4830
C
ATHR
B
55
82.095
44.802
−13.473
0.50
20.01
B
C


ATOM
4831
C
BTHR
B
55
82.126
44.817
−13.460
0.50
18.73
B
C


ATOM
4832
O
ATHR
B
55
82.031
43.600
−13.727
0.50
18.26
B
O


ATOM
4833
O
BTHR
B
55
82.081
43.618
−13.731
0.50
17.39
B
O


ATOM
4834
N
LEU
B
56
81.436
45.359
−12.458
1.00
17.88
B
N


ATOM
4835
CA
LEU
B
56
80.542
44.585
−11.603
1.00
17.45
B
C


ATOM
4836
CB
LEU
B
56
80.154
45.405
−10.388
1.00
20.45
B
C


ATOM
4837
CG
LEU
B
56
79.231
44.795
−9.353
1.00
23.80
B
C


ATOM
4838
CD1
LEU
B
56
79.791
43.459
−8.827
1.00
29.79
B
C


ATOM
4839
CD2
LEU
B
56
79.051
45.812
−8.210
1.00
26.50
B
C


ATOM
4840
C
LEU
B
56
79.283
44.148
−12.341
1.00
16.79
B
C


ATOM
4841
O
LEU
B
56
78.850
43.012
−12.204
1.00
13.84
B
O


ATOM
4842
N
LEU
B
57
78.704
45.055
−13.124
1.00
14.24
B
N


ATOM
4843
CA
LEU
B
57
77.538
44.719
−13.947
1.00
14.52
B
C


ATOM
4844
CB
LEU
B
57
77.060
45.936
−14.751
1.00
15.36
B
C


ATOM
4845
CG
LEU
B
57
76.387
47.077
−13.990
1.00
16.40
B
C


ATOM
4846
CD1
LEU
B
57
76.057
48.244
−14.958
1.00
17.56
B
C


ATOM
4847
CD2
LEU
B
57
75.162
46.595
−13.228
1.00
17.05
B
C


ATOM
4848
C
LEU
B
57
77.911
43.571
−14.881
1.00
14.74
B
C


ATOM
4849
O
LEU
B
57
77.140
42.624
−15.036
1.00
15.04
B
O


ATOM
4850
N
GLU
B
58
79.102
43.640
−15.476
1.00
13.97
B
N


ATOM
4851
CA
GLU
B
58
79.543
42.614
−16.415
1.00
17.92
B
C


ATOM
4852
CB
GLU
B
58
80.792
43.088
−17.193
1.00
23.28
B
C


ATOM
4853
CG
GLU
B
58
80.402
43.969
−18.383
1.00
35.09
B
C


ATOM
4854
CD
GLU
B
58
81.588
44.709
−19.053
1.00
48.05
B
C


ATOM
4855
OE1
GLU
B
58
82.767
44.585
−18.615
1.00
43.16
B
O


ATOM
4856
OE2
GLU
B
58
81.306
45.439
−20.028
1.00
53.26
B
O


ATOM
4857
C
GLU
B
58
79.810
41.265
−15.733
1.00
15.56
B
C


ATOM
4858
O
GLU
B
58
79.535
40.216
−16.309
1.00
14.28
B
O


ATOM
4859
N
LEU
B
59
80.376
41.302
−14.529
1.00
15.21
B
N


ATOM
4860
CA
LEU
B
59
80.525
40.113
−13.709
1.00
14.80
B
C


ATOM
4861
CB
LEU
B
59
81.201
40.430
−12.359
1.00
14.28
B
C


ATOM
4862
CG
LEU
B
59
81.338
39.248
−11.371
1.00
15.64
B
C


ATOM
4863
CD1
LEU
B
59
82.113
38.051
−11.985
1.00
15.94
B
C


ATOM
4864
CD2
LEU
B
59
82.038
39.695
−10.068
1.00
12.84
B
C


ATOM
4865
C
LEU
B
59
79.169
39.485
−13.477
1.00
12.28
B
C


ATOM
4866
O
LEU
B
59
79.000
38.318
−13.718
1.00
16.70
B
O


ATOM
4867
N
ILE
B
60
78.195
40.264
−13.037
1.00
13.86
B
N


ATOM
4868
CA
ILE
B
60
76.850
39.737
−12.792
1.00
14.00
B
C


ATOM
4869
CB
ILE
B
60
75.887
40.823
−12.271
1.00
14.84
B
C


ATOM
4870
CG1
ILE
B
60
76.307
41.274
−10.857
1.00
18.96
B
C


ATOM
4871
CD1
ILE
B
60
75.489
42.427
−10.344
1.00
17.63
B
C


ATOM
4872
CG2
ILE
B
60
74.454
40.313
−12.265
1.00
14.38
B
C


ATOM
4873
C
ILE
B
60
76.299
39.130
−14.068
1.00
13.84
B
C


ATOM
4874
O
ILE
B
60
75.797
38.018
−14.073
1.00
12.23
B
O


ATOM
4875
N
ASP
B
61
76.416
39.834
−15.167
1.00
13.78
B
N


ATOM
4876
CA
ASP
B
61
75.892
39.295
−16.431
1.00
16.74
B
C


ATOM
4877
CB
ASP
B
61
76.118
40.317
−17.541
1.00
18.93
B
C


ATOM
4878
CG
ASP
B
61
75.411
39.955
−18.827
1.00
25.31
B
C


ATOM
4879
OD1
ASP
B
61
74.304
39.329
−18.796
1.00
22.11
B
O


ATOM
4880
OD2
ASP
B
61
75.967
40.325
−19.890
1.00
28.39
B
O


ATOM
4881
C
ASP
B
61
76.562
37.969
−16.806
1.00
14.33
B
C


ATOM
4882
O
ASP
B
61
75.891
37.038
−17.262
1.00
16.51
B
O


ATOM
4883
N
ASN
B
62
77.876
37.869
−16.620
1.00
13.41
B
N


ATOM
4884
CA
ASN
B
62
78.601
36.620
−16.937
1.00
11.85
B
C


ATOM
4885
CB
ASN
B
62
80.111
36.814
−16.876
1.00
13.90
B
C


ATOM
4886
CG
ASN
B
62
80.664
37.577
−18.075
1.00
18.63
B
C


ATOM
4887
OD1
ASN
B
62
80.209
37.395
−19.169
1.00
19.52
B
O


ATOM
4888
ND2
ASN
B
62
81.674
38.399
−17.852
1.00
15.65
B
N


ATOM
4889
C
ASN
B
62
78.203
35.483
−16.006
1.00
13.00
B
C


ATOM
4890
O
ASN
B
62
77.999
34.347
−16.453
1.00
11.41
B
O


ATOM
4891
N
VAL
B
63
78.095
35.783
−14.711
1.00
11.96
B
N


ATOM
4892
CA
VAL
B
63
77.655
34.790
−13.739
1.00
11.92
B
C


ATOM
4893
CB
VAL
B
63
77.584
35.386
−12.313
1.00
12.40
B
C


ATOM
4894
CG1
VAL
B
63
76.769
34.486
−11.352
1.00
12.19
B
C


ATOM
4895
CG2
VAL
B
63
79.056
35.621
−11.770
1.00
12.49
B
C


ATOM
4896
C
VAL
B
63
76.322
34.191
−14.169
1.00
13.22
B
C


ATOM
4897
O
VAL
B
63
76.172
32.980
−14.167
1.00
16.56
B
O


ATOM
4898
N
GLN
B
64
75.383
35.046
−14.583
1.00
14.63
B
N


ATOM
4899
CA
GLN
B
64
74.054
34.606
−15.033
1.00
14.98
B
C


ATOM
4900
CB
GLN
B
64
73.090
35.802
−15.166
1.00
14.41
B
C


ATOM
4901
CG
GLN
B
64
72.771
36.400
−13.788
1.00
15.48
B
C


ATOM
4902
CD
GLN
B
64
71.677
37.443
−13.761
1.00
19.08
B
C


ATOM
4903
OE1
GLN
B
64
71.079
37.652
−12.704
1.00
21.09
B
O


ATOM
4904
NE2
GLN
B
64
71.393
38.089
−14.900
1.00
14.44
B
N


ATOM
4905
C
GLN
B
64
74.058
33.796
−16.325
1.00
13.55
B
C


ATOM
4906
O
GLN
B
64
73.489
32.713
−16.372
1.00
13.68
B
O


ATOM
4907
N
ARG
B
65
74.739
34.295
−17.346
1.00
14.94
B
N


ATOM
4908
CA
AARG
B
65
74.767
33.639
−18.651
0.50
14.67
B
C


ATOM
4909
CA
BARG
B
65
74.736
33.628
−18.642
0.50
15.55
B
C


ATOM
4910
CB
AARG
B
65
75.499
34.509
−19.676
0.50
14.34
B
C


ATOM
4911
CB
BARG
B
65
75.371
34.512
−19.716
0.50
16.50
B
C


ATOM
4912
CG
AARG
B
65
74.750
35.784
−20.027
0.50
14.56
B
C


ATOM
4913
CG
BARG
B
65
74.581
35.792
−19.964
0.50
18.15
B
C


ATOM
4914
CD
AARG
B
65
75.330
36.436
−21.285
0.50
14.40
B
C


ATOM
4915
CD
BARG
B
65
74.774
36.325
−21.386
0.50
19.88
B
C


ATOM
4916
NE
AARG
B
65
75.153
35.573
−22.455
0.50
13.78
B
N


ATOM
4917
NE
BARG
B
65
75.104
37.740
−21.397
0.50
21.26
B
N


ATOM
4918
CZ
AARG
B
65
75.573
35.871
−23.679
0.50
15.22
B
C


ATOM
4919
CZ
BARG
B
65
75.107
38.496
−22.496
0.50
20.33
B
C


ATOM
4920
NH1
AARG
B
65
76.201
37.015
−23.905
0.50
15.86
B
N


ATOM
4921
NH1
BARG
B
65
74.760
37.965
−23.662
0.50
18.24
B
N


ATOM
4922
NH2
AARG
B
65
75.356
35.027
−24.676
0.50
13.84
B
N


ATOM
4923
NH2
BARG
B
65
75.445
39.776
−22.417
0.50
16.33
B
N


ATOM
4924
C
ARG
B
65
75.410
32.264
−18.579
1.00
14.29
B
C


ATOM
4925
O
ARG
B
65
74.968
31.342
−19.254
1.00
15.76
B
O


ATOM
4926
N
LEU
B
66
76.445
32.139
−17.738
1.00
13.64
B
N


ATOM
4927
CA
LEU
B
66
77.165
30.873
−17.562
1.00
12.80
B
C


ATOM
4928
CB
LEU
B
66
78.506
31.117
−16.836
1.00
13.34
B
C


ATOM
4929
CG
LEU
B
66
79.559
31.861
−17.629
1.00
14.00
B
C


ATOM
4930
CD1
LEU
B
66
80.711
32.272
−16.735
1.00
13.19
B
C


ATOM
4931
CD2
LEU
B
66
80.026
31.017
−18.797
1.00
15.81
B
C


ATOM
4932
C
LEU
B
66
76.348
29.838
−16.785
1.00
12.95
B
C


ATOM
4933
O
LEU
B
66
76.792
28.713
−16.596
1.00
15.25
B
O


ATOM
4934
N
GLY
B
67
75.183
30.236
−16.295
1.00
12.63
B
N


ATOM
4935
CA
GLY
B
67
74.259
29.321
−15.643
1.00
11.80
B
C


ATOM
4936
C
GLY
B
67
74.279
29.330
−14.139
1.00
12.81
B
C


ATOM
4937
O
GLY
B
67
73.648
28.486
−13.528
1.00
16.75
B
O


ATOM
4938
N
LEU
B
68
74.982
30.292
−13.520
1.00
15.63
B
N


ATOM
4939
CA
LEU
B
68
75.209
30.261
−12.081
1.00
14.06
B
C


ATOM
4940
CB
LEU
B
68
76.690
30.497
−11.750
1.00
14.42
B
C


ATOM
4941
CG
LEU
B
68
77.713
29.432
−12.162
1.00
15.73
B
C


ATOM
4942
CD1
LEU
B
68
79.114
30.010
−12.224
1.00
14.70
B
C


ATOM
4943
CD2
LEU
B
68
77.694
28.268
−11.190
1.00
12.66
B
C


ATOM
4944
C
LEU
B
68
74.333
31.259
−11.311
1.00
15.70
B
C


ATOM
4945
O
LEU
B
68
74.470
31.366
−10.087
1.00
13.51
B
O


ATOM
4946
N
GLY
B
69
73.418
31.959
−11.996
1.00
15.68
B
N


ATOM
4947
CA
GLY
B
69
72.570
32.988
−11.342
1.00
17.75
B
C


ATOM
4948
C
GLY
B
69
71.803
32.506
−10.116
1.00
18.82
B
C


ATOM
4949
O
GLY
B
69
71.745
33.208
−9.086
1.00
21.69
B
O


ATOM
4950
N
TYR
B
70
71.282
31.280
−10.210
1.00
15.94
B
N


ATOM
4951
CA
TYR
B
70
70.471
30.667
−9.161
1.00
19.27
B
C


ATOM
4952
CB
TYR
B
70
69.927
29.293
−9.621
1.00
17.09
B
C


ATOM
4953
CG
TYR
B
70
70.942
28.141
−9.574
1.00
15.13
B
C


ATOM
4954
CD1
TYR
B
70
71.898
27.965
−10.586
1.00
14.78
B
C


ATOM
4955
CE1
TYR
B
70
72.831
26.932
−10.541
1.00
14.99
B
C


ATOM
4956
CZ
TYR
B
70
72.823
26.068
−9.445
1.00
19.35
B
C


ATOM
4957
OH
TYR
B
70
73.748
25.067
−9.313
1.00
15.46
B
O


ATOM
4958
CE2
TYR
B
70
71.869
26.238
−8.426
1.00
18.36
B
C


ATOM
4959
CD2
TYR
B
70
70.959
27.259
−8.500
1.00
17.04
B
C


ATOM
4960
C
TYR
B
70
71.268
30.533
−7.851
1.00
21.65
B
C


ATOM
4961
O
TYR
B
70
70.673
30.525
−6.786
1.00
25.79
B
O


ATOM
4962
N
ARG
B
71
72.603
30.401
−7.943
1.00
18.29
B
N


ATOM
4963
CA
AARG
B
71
73.446
30.173
−6.776
0.50
19.57
B
C


ATOM
4964
CA
BARG
B
71
73.457
30.183
−6.759
0.50
18.98
B
C


ATOM
4965
CB
AARG
B
71
74.713
29.448
−7.251
0.50
20.81
B
C


ATOM
4966
CB
BARG
B
71
74.799
29.522
−7.129
0.50
19.07
B
C


ATOM
4967
CG
AARG
B
71
75.669
29.003
−6.186
0.50
19.46
B
C


ATOM
4968
CG
BARG
B
71
74.723
28.171
−7.779
0.50
17.63
B
C


ATOM
4969
CD
AARG
B
71
76.649
27.983
−6.782
0.50
18.97
B
C


ATOM
4970
CD
BARG
B
71
76.055
27.469
−7.708
0.50
15.61
B
C


ATOM
4971
NE
AARG
B
71
75.968
26.746
−7.137
0.50
15.99
B
N


ATOM
4972
NE
BARG
B
71
76.367
27.021
−6.358
0.50
15.15
B
N


ATOM
4973
CZ
AARG
B
71
75.615
25.816
−6.259
0.50
17.57
B
C


ATOM
4974
CZ
BARG
B
71
75.804
25.972
−5.769
0.50
15.78
B
C


ATOM
4975
NH1
AARG
B
71
74.986
24.717
−6.670
0.50
17.39
B
N


ATOM
4976
NH1
BARG
B
71
74.896
25.247
−6.419
0.50
16.27
B
N


ATOM
4977
NH2
AARG
B
71
75.894
25.982
−4.967
0.50
18.25
B
N


ATOM
4978
NH2
BARG
B
71
76.150
25.645
−4.524
0.50
14.04
B
N


ATOM
4979
C
ARG
B
71
73.814
31.488
−6.067
1.00
18.17
B
C


ATOM
4980
O
ARG
B
71
74.263
31.476
−4.940
1.00
17.84
B
O


ATOM
4981
N
PHE
B
72
73.658
32.606
−6.763
1.00
16.59
B
N


ATOM
4982
CA
PHE
B
72
74.128
33.897
−6.263
1.00
17.05
B
C


ATOM
4983
CB
PHE
B
72
75.302
34.386
−7.148
1.00
17.06
B
C


ATOM
4984
CG
PHE
B
72
76.518
33.505
−7.065
1.00
15.19
B
C


ATOM
4985
CD1
PHE
B
72
77.291
33.492
−5.908
1.00
14.85
B
C


ATOM
4986
CE1
PHE
B
72
78.382
32.681
−5.810
1.00
13.25
B
C


ATOM
4987
CZ
PHE
B
72
78.729
31.844
−6.882
1.00
13.18
B
C


ATOM
4988
CE2
PHE
B
72
77.945
31.823
−8.018
1.00
13.45
B
C


ATOM
4989
CD2
PHE
B
72
76.848
32.659
−8.102
1.00
13.58
B
C


ATOM
4990
C
PHE
B
72
73.041
34.961
−6.200
1.00
17.89
B
C


ATOM
4991
O
PHE
B
72
73.344
36.158
−6.201
1.00
15.78
B
O


ATOM
4992
N
GLU
B
73
71.782
34.542
−6.127
1.00
21.33
B
N


ATOM
4993
CA
GLU
B
73
70.708
35.501
−6.306
1.00
24.20
B
C


ATOM
4994
CB
GLU
B
73
69.324
34.862
−6.298
1.00
27.63
B
C


ATOM
4995
CG
GLU
B
73
68.238
35.887
−6.529
1.00
32.36
B
C


ATOM
4996
CD
GLU
B
73
66.884
35.260
−6.841
1.00
44.63
B
C


ATOM
4997
OE1
GLU
B
73
66.833
34.092
−7.280
1.00
43.71
B
O


ATOM
4998
OE2
GLU
B
73
65.872
35.955
−6.653
1.00
43.69
B
O


ATOM
4999
C
GLU
B
73
70.731
36.591
−5.264
1.00
23.08
B
C


ATOM
5000
O
GLU
B
73
70.546
37.755
−5.604
1.00
21.72
B
O


ATOM
5001
N
SER
B
74
70.958
36.262
−4.003
1.00
21.31
B
N


ATOM
5002
CA
SER
B
74
70.868
37.305
−3.025
1.00
25.45
B
C


ATOM
5003
CB
SER
B
74
70.662
36.730
−1.637
1.00
30.25
B
C


ATOM
5004
OG
SER
B
74
71.856
36.148
−1.173
1.00
38.94
B
O


ATOM
5005
C
SER
B
74
72.120
38.207
−3.126
1.00
25.84
B
C


ATOM
5006
O
SER
B
74
72.064
39.392
−2.813
1.00
23.71
B
O


ATOM
5007
N
ASP
B
75
73.255
37.646
−3.554
1.00
20.01
B
N


ATOM
5008
CA
ASP
B
75
74.460
38.467
−3.744
1.00
18.98
B
C


ATOM
5009
CB
ASP
B
75
75.678
37.580
−4.059
1.00
18.31
B
C


ATOM
5010
CG
ASP
B
75
75.953
36.557
−2.962
1.00
24.11
B
C


ATOM
5011
OD1
ASP
B
75
76.184
36.968
−1.810
1.00
21.46
B
O


ATOM
5012
OD2
ASP
B
75
75.916
35.339
−3.246
1.00
31.41
B
O


ATOM
5013
C
ASP
B
75
74.228
39.482
−4.879
1.00
13.74
B
C


ATOM
5014
O
ASP
B
75
74.605
40.657
−4.777
1.00
16.30
B
O


ATOM
5015
N
ILE
B
76
73.635
38.997
−5.969
1.00
15.12
B
N


ATOM
5016
CA
ILE
B
76
73.334
39.841
−7.134
1.00
15.07
B
C


ATOM
5017
CB
ILE
B
76
72.804
39.005
−8.296
1.00
15.73
B
C


ATOM
5018
CG1
ILE
B
76
73.932
38.106
−8.838
1.00
16.42
B
C


ATOM
5019
CD1
ILE
B
76
73.471
36.938
−9.708
1.00
16.94
B
C


ATOM
5020
CG2
ILE
B
76
72.198
39.922
−9.393
1.00
12.45
B
C


ATOM
5021
C
ILE
B
76
72.338
40.932
−6.736
1.00
15.52
B
C


ATOM
5022
O
ILE
B
76
72.543
42.077
−7.029
1.00
13.57
B
O


ATOM
5023
N
ARG
B
77
71.294
40.586
−5.998
1.00
17.87
B
N


ATOM
5024
CA
AARG
B
77
70.303
41.571
−5.548
0.50
19.33
B
C


ATOM
5025
CA
BARG
B
77
70.327
41.607
−5.606
0.50
21.73
B
C


ATOM
5026
CB
AARG
B
77
69.183
40.903
−4.730
0.50
18.50
B
C


ATOM
5027
CB
BARG
B
77
69.065
41.007
−4.978
0.50
24.67
B
C


ATOM
5028
CG
AARG
B
77
68.257
39.962
−5.492
0.50
18.79
B
C


ATOM
5029
CG
BARG
B
77
67.932
40.869
−5.962
0.50
31.93
B
C


ATOM
5030
CD
AARG
B
77
67.061
39.485
−4.594
0.50
18.26
B
C


ATOM
5031
CD
BARG
B
77
66.571
41.085
−5.291
0.50
41.21
B
C


ATOM
5032
NE
AARG
B
77
66.301
40.638
−4.130
0.50
22.01
B
N


ATOM
5033
NE
BARG
B
77
66.552
40.594
−3.914
0.50
43.53
B
N


ATOM
5034
CZ
AARG
B
77
65.456
41.338
−4.891
0.50
23.05
B
C


ATOM
5035
CZ
BARG
B
77
66.554
39.311
−3.580
0.50
41.07
B
C


ATOM
5036
NH1
AARG
B
77
65.227
40.967
−6.143
0.50
18.41
B
N


ATOM
5037
NH1
BARG
B
77
66.580
38.379
−4.520
0.50
43.20
B
N


ATOM
5038
NH2
AARG
B
77
64.833
42.401
−4.392
0.50
23.08
B
N


ATOM
5039
NH2
BARG
B
77
66.534
38.958
−2.304
0.50
51.19
B
N


ATOM
5040
C
ARG
B
77
70.952
42.656
−4.697
1.00
19.64
B
C


ATOM
5041
O
ARG
B
77
70.680
43.840
−4.863
1.00
22.88
B
O


ATOM
5042
N
GLY
B
78
71.819
42.240
−3.775
1.00
20.09
B
N


ATOM
5043
CA
GLY
B
78
72.515
43.204
−2.929
1.00
20.98
B
C


ATOM
5044
C
GLY
B
78
73.364
44.174
−3.731
1.00
20.75
B
C


ATOM
5045
O
GLY
B
78
73.381
45.377
−3.461
1.00
22.15
B
O


ATOM
5046
N
ALA
B
79
74.089
43.655
−4.720
1.00
18.78
B
N


ATOM
5047
CA
ALA
B
79
74.924
44.489
−5.572
1.00
18.42
B
C


ATOM
5048
CB
ALA
B
79
75.778
43.612
−6.495
1.00
17.86
B
C


ATOM
5049
C
ALA
B
79
74.072
45.457
−6.361
1.00
15.85
B
C


ATOM
5050
O
ALA
B
79
74.398
46.630
−6.489
1.00
14.18
B
O


ATOM
5051
N
LEU
B
80
72.953
44.983
−6.896
1.00
17.36
B
N


ATOM
5052
CA
LEU
B
80
72.102
45.859
−7.714
1.00
18.93
B
C


ATOM
5053
CB
LEU
B
80
71.048
45.045
−8.449
1.00
17.55
B
C


ATOM
5054
CG
LEU
B
80
71.587
44.131
−9.546
1.00
17.15
B
C


ATOM
5055
CD1
LEU
B
80
70.465
43.255
−10.060
1.00
16.24
B
C


ATOM
5056
CD2
LEU
B
80
72.209
44.964
−10.668
1.00
15.59
B
C


ATOM
5057
C
LEU
B
80
71.429
46.957
−6.878
1.00
22.83
B
C


ATOM
5058
O
LEU
B
80
71.268
48.090
−7.337
1.00
20.36
B
O


ATOM
5059
N
ASP
B
81
71.031
46.607
−5.660
1.00
26.31
B
N


ATOM
5060
CA
ASP
B
81
70.407
47.565
−4.753
1.00
32.97
B
C


ATOM
5061
CB
ASP
B
81
69.943
46.842
−3.483
1.00
36.50
B
C


ATOM
5062
CG
ASP
B
81
69.066
47.710
−2.608
1.00
44.23
B
C


ATOM
5063
OD1
ASP
B
81
67.937
48.029
−3.026
1.00
48.95
B
O


ATOM
5064
OD2
ASP
B
81
69.504
48.068
−1.496
1.00
48.03
B
O


ATOM
5065
C
ASP
B
81
71.376
48.702
−4.422
1.00
28.91
B
C


ATOM
5066
O
ASP
B
81
71.000
49.874
−4.421
1.00
31.14
B
O


ATOM
5067
N
ARG
B
82
72.624
48.346
−4.126
1.00
27.25
B
N


ATOM
5068
CA
ARG
B
82
73.659
49.337
−3.836
1.00
28.22
B
C


ATOM
5069
CB
ARG
B
82
74.954
48.676
−3.355
1.00
28.94
B
C


ATOM
5070
CG
ARG
B
82
74.907
48.182
−1.923
1.00
36.06
B
C


ATOM
5071
CD
ARG
B
82
76.297
47.764
−1.463
1.00
40.01
B
C


ATOM
5072
NE
ARG
B
82
76.837
46.648
−2.246
1.00
38.93
B
N


ATOM
5073
CZ
ARG
B
82
76.499
45.365
−2.078
1.00
39.77
B
C


ATOM
5074
NH1
ARG
B
82
75.598
45.013
−1.159
1.00
41.64
B
N


ATOM
5075
NH2
ARG
B
82
77.057
44.422
−2.839
1.00
30.99
B
N


ATOM
5076
C
ARG
B
82
73.961
50.193
−5.051
1.00
24.69
B
C


ATOM
5077
O
ARG
B
82
74.162
51.379
−4.920
1.00
24.76
B
O


ATOM
5078
N
PHE
B
83
73.977
49.587
−6.235
1.00
23.39
B
N


ATOM
5079
CA
PHE
B
83
74.153
50.328
−7.480
1.00
22.29
B
C


ATOM
5080
CB
PHE
B
83
74.075
49.369
−8.666
1.00
24.15
B
C


ATOM
5081
CG
PHE
B
83
74.217
50.024
−10.007
1.00
23.55
B
C


ATOM
5082
CD1
PHE
B
83
75.406
50.644
−10.367
1.00
30.81
B
C


ATOM
5083
CE1
PHE
B
83
75.551
51.249
−11.615
1.00
26.93
B
C


ATOM
5084
CZ
PHE
B
83
74.501
51.224
−12.512
1.00
29.70
B
C


ATOM
5085
CE2
PHE
B
83
73.317
50.621
−12.156
1.00
25.36
B
C


ATOM
5086
CD2
PHE
B
83
73.184
50.021
−10.908
1.00
25.59
B
C


ATOM
5087
C
PHE
B
83
73.080
51.402
−7.617
1.00
28.13
B
C


ATOM
5088
O
PHE
B
83
73.362
52.530
−8.036
1.00
32.31
B
O


ATOM
5089
N
VAL
B
84
71.850
51.063
−7.264
1.00
25.80
B
N


ATOM
5090
CA
VAL
B
84
70.783
52.044
−7.360
1.00
32.46
B
C


ATOM
5091
CB
VAL
B
84
69.401
51.419
−7.237
1.00
34.87
B
C


ATOM
5092
CG1
VAL
B
84
68.364
52.505
−6.892
1.00
35.85
B
C


ATOM
5093
CG2
VAL
B
84
69.052
50.687
−8.530
1.00
26.81
B
C


ATOM
5094
C
VAL
B
84
70.960
53.073
−6.266
1.00
36.84
B
C


ATOM
5095
O
VAL
B
84
71.291
54.206
−6.550
1.00
30.70
B
O


ATOM
5096
N
SER
B
85
70.790
52.647
−5.017
1.00
43.24
B
N


ATOM
5097
CA
SER
B
85
70.754
53.564
−3.876
1.00
42.00
B
C


ATOM
5098
CB
SER
B
85
70.548
52.782
−2.575
1.00
41.82
B
C


ATOM
5099
OG
SER
B
85
71.758
52.172
−2.170
1.00
45.68
B
O


ATOM
5100
C
SER
B
85
71.987
54.478
−3.762
1.00
42.81
B
C


ATOM
5101
O
SER
B
85
71.910
55.543
−3.151
1.00
47.04
B
O


ATOM
5102
N
SER
B
86
73.110
54.090
−4.356
1.00
35.93
B
N


ATOM
5103
CA
SER
B
86
74.283
54.951
−4.359
1.00
33.92
B
C


ATOM
5104
CB
SER
B
86
75.548
54.112
−4.499
1.00
32.14
B
C


ATOM
5105
OG
SER
B
86
75.729
53.707
−5.842
1.00
33.64
B
O


ATOM
5106
C
SER
B
86
74.268
56.013
−5.478
1.00
38.88
B
C


ATOM
5107
O
SER
B
86
75.231
56.746
−5.622
1.00
39.84
B
O


ATOM
5108
N
GLY
B
87
73.200
56.086
−6.272
1.00
39.23
B
N


ATOM
5109
CA
GLY
B
87
73.160
56.987
−7.436
1.00
36.38
B
C


ATOM
5110
C
GLY
B
87
73.901
56.455
−8.659
1.00
40.92
B
C


ATOM
5111
O
GLY
B
87
74.032
57.161
−9.663
1.00
31.51
B
O


ATOM
5112
N
GLY
B
88
74.369
55.203
−8.596
1.00
39.83
B
N


ATOM
5113
CA
GLY
B
88
75.122
54.595
−9.692
1.00
31.43
B
C


ATOM
5114
C
GLY
B
88
74.314
54.463
−10.962
1.00
33.31
B
C


ATOM
5115
O
GLY
B
88
74.849
54.605
−12.066
1.00
37.29
B
O


ATOM
5116
N
PHE
B
89
73.019
54.205
−10.824
1.00
35.85
B
N


ATOM
5117
CA
PHE
B
89
72.158
54.073
−12.009
1.00
36.71
B
C


ATOM
5118
CB
PHE
B
89
70.828
53.383
−11.649
1.00
31.01
B
C


ATOM
5119
CG
PHE
B
89
69.943
53.122
−12.832
1.00
32.04
B
C


ATOM
5120
CD1
PHE
B
89
70.408
52.394
−13.927
1.00
30.41
B
C


ATOM
5121
CE1
PHE
B
89
69.592
52.172
−15.029
1.00
29.24
B
C


ATOM
5122
CZ
PHE
B
89
68.316
52.656
−15.040
1.00
30.11
B
C


ATOM
5123
CE2
PHE
B
89
67.844
53.383
−13.961
1.00
33.17
B
C


ATOM
5124
CD2
PHE
B
89
68.655
53.612
−12.866
1.00
31.62
B
C


ATOM
5125
C
PHE
B
89
71.966
55.438
−12.716
1.00
34.35
B
C


ATOM
5126
O
PHE
B
89
71.972
55.512
−13.944
1.00
35.68
B
O


ATOM
5127
N
ASP
B
90
71.831
56.515
−11.951
1.00
47.99
B
N


ATOM
5128
CA
ASP
B
90
71.864
57.873
−12.534
1.00
47.41
B
C


ATOM
5129
CB
ASP
B
90
71.851
58.947
−11.450
1.00
63.15
B
C


ATOM
5130
CG
ASP
B
90
70.460
59.379
−11.085
1.00
75.50
B
C


ATOM
5131
OD1
ASP
B
90
69.558
58.515
−11.089
1.00
55.04
B
O


ATOM
5132
OD2
ASP
B
90
70.273
60.583
−10.798
1.00
83.17
B
O


ATOM
5133
C
ASP
B
90
73.099
58.096
−13.376
1.00
39.65
B
C


ATOM
5134
O
ASP
B
90
72.997
58.504
−14.532
1.00
47.01
B
O


ATOM
5135
N
ALA
B
91
74.256
57.840
−12.770
1.00
38.64
B
N


ATOM
5136
CA
ALA
B
91
75.557
58.067
−13.402
1.00
37.96
B
C


ATOM
5137
CB
ALA
B
91
76.689
57.509
−12.536
1.00
36.86
B
C


ATOM
5138
C
ALA
B
91
75.615
57.459
−14.790
1.00
38.02
B
C


ATOM
5139
O
ALA
B
91
75.941
58.162
−15.753
1.00
26.33
B
O


ATOM
5140
N
VAL
B
92
75.271
56.169
−14.912
1.00
32.42
B
N


ATOM
5141
CA
VAL
B
92
75.437
55.495
−16.197
1.00
28.95
B
C


ATOM
5142
CB
VAL
B
92
75.332
53.942
−16.123
1.00
31.66
B
C


ATOM
5143
CG1
VAL
B
92
76.404
53.387
−15.168
1.00
37.05
B
C


ATOM
5144
CG2
VAL
B
92
73.933
53.505
−15.726
1.00
26.45
B
C


ATOM
5145
C
VAL
B
92
74.499
56.067
−17.256
1.00
24.25
B
C


ATOM
5146
O
VAL
B
92
74.873
56.119
−18.422
1.00
26.79
B
O


ATOM
5147
N
THR
B
93
73.311
56.522
−16.864
1.00
27.09
B
N


ATOM
5148
CA
THR
B
93
72.349
57.050
−17.847
1.00
29.51
B
C


ATOM
5149
CB
THR
B
93
70.917
57.196
−17.276
1.00
31.52
B
C


ATOM
5150
OG1
THR
B
93
70.912
58.154
−16.215
1.00
37.00
B
O


ATOM
5151
CG2
THR
B
93
70.381
55.842
−16.756
1.00
30.63
B
C


ATOM
5152
C
THR
B
93
72.813
58.375
−18.497
1.00
28.69
B
C


ATOM
5153
O
THR
B
93
72.271
58.769
−19.530
1.00
26.16
B
O


ATOM
5154
N
LYS
B
94
73.830
59.021
−17.923
1.00
34.87
B
N


ATOM
5155
CA
LYS
B
94
74.462
60.213
−18.540
1.00
39.05
B
C


ATOM
5156
CB
LYS
B
94
74.944
61.223
−17.488
1.00
40.76
B
C


ATOM
5157
CG
LYS
B
94
74.018
61.479
−16.315
1.00
48.73
B
C


ATOM
5158
CD
LYS
B
94
72.655
61.989
−16.739
1.00
55.16
B
C


ATOM
5159
CE
LYS
B
94
71.900
62.521
−15.529
1.00
59.95
B
C


ATOM
5160
NZ
LYS
B
94
70.454
62.656
−15.807
1.00
68.55
B
N


ATOM
5161
C
LYS
B
94
75.673
59.870
−19.400
1.00
37.62
B
C


ATOM
5162
O
LYS
B
94
76.036
60.639
−20.278
1.00
42.15
B
O


ATOM
5163
N
THR
B
95
76.315
58.739
−19.123
1.00
35.13
B
N


ATOM
5164
CA
THR
B
95
77.645
58.453
−19.667
1.00
31.61
B
C


ATOM
5165
CB
THR
B
95
78.643
58.170
−18.522
1.00
30.78
B
C


ATOM
5166
OG1
THR
B
95
78.293
56.950
−17.848
1.00
30.43
B
O


ATOM
5167
CG2
THR
B
95
78.647
59.332
−17.500
1.00
30.18
B
C


ATOM
5168
C
THR
B
95
77.696
57.305
−20.690
1.00
33.47
B
C


ATOM
5169
O
THR
B
95
78.454
57.390
−21.659
1.00
33.96
B
O


ATOM
5170
N
SER
B
96
76.884
56.252
−20.487
1.00
30.71
B
N


ATOM
5171
CA
SER
B
96
77.113
54.954
−21.139
1.00
26.68
B
C


ATOM
5172
CB
SER
B
96
77.999
54.103
−20.208
1.00
26.33
B
C


ATOM
5173
OG
SER
B
96
78.094
52.750
−20.606
1.00
28.02
B
O


ATOM
5174
C
SER
B
96
75.801
54.201
−21.526
1.00
28.35
B
C


ATOM
5175
O
SER
B
96
74.996
53.790
−20.658
1.00
21.83
B
O


ATOM
5176
N
LEU
B
97
75.608
54.003
−22.832
1.00
24.62
B
N


ATOM
5177
CA
LEU
B
97
74.475
53.217
−23.336
1.00
26.38
B
C


ATOM
5178
CB
LEU
B
97
74.395
53.258
−24.865
1.00
24.22
B
C


ATOM
5179
CG
LEU
B
97
73.301
52.390
−25.504
1.00
23.98
B
C


ATOM
5180
CD1
LEU
B
97
71.912
52.882
−25.098
1.00
23.33
B
C


ATOM
5181
CD2
LEU
B
97
73.456
52.362
−27.022
1.00
24.44
B
C


ATOM
5182
C
LEU
B
97
74.582
51.770
−22.870
1.00
25.26
B
C


ATOM
5183
O
LEU
B
97
73.606
51.211
−22.399
1.00
23.11
B
O


ATOM
5184
N
HIS
B
98
75.762
51.174
−23.029
1.00
23.74
B
N


ATOM
5185
CA
HIS
B
98
76.022
49.808
−22.571
1.00
23.36
B
C


ATOM
5186
CB
HIS
B
98
77.471
49.427
−22.857
1.00
23.90
B
C


ATOM
5187
CG
HIS
B
98
77.854
48.056
−22.369
1.00
28.17
B
C


ATOM
5188
ND1
HIS
B
98
77.018
46.996
−22.448
1.00
32.87
B
N


ATOM
5189
CE1
HIS
B
98
77.624
45.910
−21.935
1.00
31.82
B
C


ATOM
5190
NE2
HIS
B
98
78.855
46.271
−21.537
1.00
38.65
B
N


ATOM
5191
CD2
HIS
B
98
79.029
47.588
−21.795
1.00
30.71
B
C


ATOM
5192
C
HIS
B
98
75.711
49.673
−21.103
1.00
22.84
B
C


ATOM
5193
O
HIS
B
98
74.971
48.773
−20.701
1.00
20.22
B
O


ATOM
5194
N
GLY
B
99
76.250
50.572
−20.283
1.00
19.58
B
N


ATOM
5195
CA
GLY
B
99
75.972
50.557
−18.836
1.00
19.66
B
C


ATOM
5196
C
GLY
B
99
74.482
50.668
−18.520
1.00
20.65
B
C


ATOM
5197
O
GLY
B
99
73.955
49.949
−17.650
1.00
16.40
B
O


ATOM
5198
N
THR
B
100
73.803
51.564
−19.236
1.00
16.08
B
N


ATOM
5199
CA
THR
B
100
72.399
51.804
−19.018
1.00
16.75
B
C


ATOM
5200
CB
THR
B
100
71.936
53.097
−19.719
1.00
18.78
B
C


ATOM
5201
OG1
THR
B
100
72.623
54.221
−19.144
1.00
22.13
B
O


ATOM
5202
CG2
THR
B
100
70.458
53.296
−19.554
1.00
18.90
B
C


ATOM
5203
C
THR
B
100
71.544
50.611
−19.462
1.00
18.44
B
C


ATOM
5204
O
THR
B
100
70.656
50.192
−18.725
1.00
16.59
B
O


ATOM
5205
N
ALA
B
101
71.840
50.058
−20.637
1.00
17.28
B
N


ATOM
5206
CA
ALA
B
101
71.060
48.965
−21.187
1.00
19.07
B
C


ATOM
5207
CB
ALA
B
101
71.445
48.722
−22.648
1.00
18.01
B
C


ATOM
5208
C
ALA
B
101
71.268
47.705
−20.333
1.00
16.43
B
C


ATOM
5209
O
ALA
B
101
70.329
46.972
−20.047
1.00
18.06
B
O


ATOM
5210
N
LEU
B
102
72.500
47.470
−19.915
1.00
15.43
B
N


ATOM
5211
CA
LEU
B
102
72.785
46.284
−19.108
1.00
16.88
B
C


ATOM
5212
CB
LEU
B
102
74.307
46.070
−18.984
1.00
18.21
B
C


ATOM
5213
CG
LEU
B
102
74.770
44.887
−18.124
1.00
16.60
B
C


ATOM
5214
CD1
LEU
B
102
74.090
43.606
−18.521
1.00
17.56
B
C


ATOM
5215
CD2
LEU
B
102
76.308
44.767
−18.207
1.00
20.27
B
C


ATOM
5216
C
LEU
B
102
72.126
46.418
−17.725
1.00
16.97
B
C


ATOM
5217
O
LEU
B
102
71.450
45.499
−17.238
1.00
14.04
B
O


ATOM
5218
N
SER
B
103
72.304
47.574
−17.096
1.00
18.21
B
N


ATOM
5219
CA
SER
B
103
71.771
47.752
−15.763
1.00
17.35
B
C


ATOM
5220
CB
SER
B
103
72.333
49.003
−15.089
1.00
18.75
B
C


ATOM
5221
OG
SER
B
103
72.042
50.151
−15.843
1.00
18.72
B
O


ATOM
5222
C
SER
B
103
70.242
47.749
−15.785
1.00
18.14
B
C


ATOM
5223
O
SER
B
103
69.630
47.214
−14.885
1.00
18.58
B
O


ATOM
5224
N
PHE
B
104
69.641
48.329
−16.818
1.00
18.14
B
N


ATOM
5225
CA
PHE
B
104
68.199
48.322
−16.971
1.00
18.85
B
C


ATOM
5226
CB
PHE
B
104
67.813
49.009
−18.289
1.00
19.22
B
C


ATOM
5227
CG
PHE
B
104
66.323
49.037
−18.548
1.00
19.65
B
C


ATOM
5228
CD1
PHE
B
104
65.693
47.984
−19.200
1.00
20.74
B
C


ATOM
5229
CE1
PHE
B
104
64.314
47.998
−19.439
1.00
19.71
B
C


ATOM
5230
CZ
PHE
B
104
63.567
49.056
−19.033
1.00
18.71
B
C


ATOM
5231
CE2
PHE
B
104
64.183
50.127
−18.407
1.00
24.37
B
C


ATOM
5232
CD2
PHE
B
104
65.563
50.114
−18.167
1.00
22.21
B
C


ATOM
5233
C
PHE
B
104
67.689
46.882
−16.973
1.00
17.17
B
C


ATOM
5234
O
PHE
B
104
66.741
46.526
−16.245
1.00
16.32
B
O


ATOM
5235
N
ARG
B
105
68.336
46.043
−17.778
1.00
13.42
B
N


ATOM
5236
CA
ARG
B
105
67.919
44.645
−17.873
1.00
15.63
B
C


ATOM
5237
CB
ARG
B
105
68.641
43.918
−19.024
1.00
13.02
B
C


ATOM
5238
CG
ARG
B
105
68.333
42.452
−19.081
1.00
14.74
B
C


ATOM
5239
CD
ARG
B
105
68.877
41.808
−20.362
1.00
16.05
B
C


ATOM
5240
NE
ARG
B
105
70.330
41.836
−20.500
1.00
16.37
B
N


ATOM
5241
CZ
ARG
B
105
71.161
40.984
−19.914
1.00
17.27
B
C


ATOM
5242
NH1
ARG
B
105
70.697
40.030
−19.116
1.00
17.40
B
N


ATOM
5243
NH2
ARG
B
105
72.454
41.085
−20.139
1.00
20.81
B
N


ATOM
5244
C
ARG
B
105
68.075
43.891
−16.551
1.00
15.32
B
C


ATOM
5245
O
ARG
B
105
67.152
43.225
−16.110
1.00
14.27
B
O


ATOM
5246
N
LEU
B
106
69.229
44.025
−15.901
1.00
12.51
B
N


ATOM
5247
CA
LEU
B
106
69.477
43.304
−14.683
1.00
13.49
B
C


ATOM
5248
CB
LEU
B
106
70.965
43.433
−14.243
1.00
12.09
B
C


ATOM
5249
CG
LEU
B
106
72.010
42.909
−15.238
1.00
12.53
B
C


ATOM
5250
CD1
LEU
B
106
73.418
43.201
−14.689
1.00
10.73
B
C


ATOM
5251
CD2
LEU
B
106
71.819
41.373
−15.502
1.00
11.52
B
C


ATOM
5252
C
LEU
B
106
68.525
43.797
−13.602
1.00
12.64
B
C


ATOM
5253
O
LEU
B
106
67.965
42.992
−12.851
1.00
13.50
B
O


ATOM
5254
N
LEU
B
107
68.343
45.107
−13.521
1.00
16.05
B
N


ATOM
5255
CA
LEU
B
107
67.468
45.692
−12.507
1.00
16.98
B
C


ATOM
5256
CB
LEU
B
107
67.541
47.222
−12.557
1.00
18.29
B
C


ATOM
5257
CG
LEU
B
107
68.810
47.803
−11.933
1.00
19.58
B
C


ATOM
5258
CD1
LEU
B
107
69.009
49.257
−12.356
1.00
18.58
B
C


ATOM
5259
CD2
LEU
B
107
68.701
47.653
−10.424
1.00
21.10
B
C


ATOM
5260
C
LEU
B
107
66.038
45.203
−12.708
1.00
17.38
B
C


ATOM
5261
O
LEU
B
107
65.402
44.690
−11.777
1.00
16.00
B
O


ATOM
5262
N
ARG
B
108
65.546
45.305
−13.936
1.00
16.53
B
N


ATOM
5263
CA
ARG
B
108
64.191
44.837
−14.199
1.00
16.64
B
C


ATOM
5264
CB
ARG
B
108
63.695
45.245
−15.585
1.00
17.67
B
C


ATOM
5265
CG
ARG
B
108
62.248
44.770
−15.818
1.00
22.71
B
C


ATOM
5266
CD
ARG
B
108
61.545
45.480
−16.945
1.00
22.49
B
C


ATOM
5267
NE
ARG
B
108
61.208
46.877
−16.656
1.00
17.35
B
N


ATOM
5268
CZ
ARG
B
108
60.598
47.687
−17.524
1.00
19.22
B
C


ATOM
5269
NH1
ARG
B
108
60.236
47.262
−18.727
1.00
18.94
B
N


ATOM
5270
NH2
ARG
B
108
60.345
48.935
−17.195
1.00
18.38
B
N


ATOM
5271
C
ARG
B
108
64.068
43.326
−13.992
1.00
15.93
B
C


ATOM
5272
O
ARG
B
108
63.082
42.852
−13.439
1.00
15.19
B
O


ATOM
5273
N
GLN
B
109
65.066
42.552
−14.403
1.00
16.08
B
N


ATOM
5274
CA
GLN
B
109
65.036
41.101
−14.168
1.00
16.94
B
C


ATOM
5275
CB
GLN
B
109
66.363
40.481
−14.657
1.00
18.02
B
C


ATOM
5276
CG
GLN
B
109
66.470
38.977
−14.403
1.00
20.52
B
C


ATOM
5277
CD
GLN
B
109
67.881
38.431
−14.647
1.00
21.88
B
C


ATOM
5278
OE1
GLN
B
109
68.756
39.140
−15.161
1.00
17.04
B
O


ATOM
5279
NE2
GLN
B
109
68.104
37.159
−14.259
1.00
19.73
B
N


ATOM
5280
C
GLN
B
109
64.829
40.744
−12.667
1.00
17.41
B
C


ATOM
5281
O
GLN
B
109
64.139
39.781
−12.307
1.00
16.39
B
O


ATOM
5282
N
HIS
B
110
65.462
41.524
−11.809
1.00
16.32
B
N


ATOM
5283
CA
HIS
B
110
65.458
41.278
−10.395
1.00
19.70
B
C


ATOM
5284
CB
HIS
B
110
66.885
41.520
−9.843
1.00
18.76
B
C


ATOM
5285
CG
HIS
B
110
67.825
40.397
−10.201
1.00
19.50
B
C


ATOM
5286
ND1
HIS
B
110
67.819
39.207
−9.534
1.00
20.72
B
N


ATOM
5287
CE1
HIS
B
110
68.724
38.374
−10.105
1.00
20.86
B
C


ATOM
5288
NE2
HIS
B
110
69.259
39.005
−11.175
1.00
18.25
B
N


ATOM
5289
CD2
HIS
B
110
68.732
40.260
−11.251
1.00
15.44
B
C


ATOM
5290
C
HIS
B
110
64.378
42.100
−9.712
1.00
23.42
B
C


ATOM
5291
O
HIS
B
110
64.443
42.349
−8.519
1.00
23.26
B
O


ATOM
5292
N
GLY
B
111
63.376
42.531
−10.475
1.00
21.81
B
N


ATOM
5293
CA
GLY
B
111
62.171
43.131
−9.890
1.00
20.16
B
C


ATOM
5294
C
GLY
B
111
62.273
44.581
−9.440
1.00
26.24
B
C


ATOM
5295
O
GLY
B
111
61.360
45.060
−8.790
1.00
27.12
B
O


ATOM
5296
N
PHE
B
112
63.376
45.271
−9.753
1.00
18.84
B
N


ATOM
5297
CA
PHE
B
112
63.499
46.674
−9.445
1.00
21.69
B
C


ATOM
5298
CB
PHE
B
112
64.953
47.135
−9.492
1.00
22.76
B
C


ATOM
5299
CG
PHE
B
112
65.794
46.565
−8.408
1.00
24.54
B
C


ATOM
5300
CD1
PHE
B
112
66.375
45.310
−8.545
1.00
23.28
B
C


ATOM
5301
CE1
PHE
B
112
67.165
44.784
−7.549
1.00
25.68
B
C


ATOM
5302
CZ
PHE
B
112
67.357
45.492
−6.378
1.00
30.15
B
C


ATOM
5303
CE2
PHE
B
112
66.771
46.735
−6.222
1.00
30.82
B
C


ATOM
5304
CD2
PHE
B
112
65.999
47.270
−7.233
1.00
29.06
B
C


ATOM
5305
C
PHE
B
112
62.698
47.477
−10.446
1.00
23.68
B
C


ATOM
5306
O
PHE
B
112
62.494
47.050
−11.584
1.00
24.90
B
O


ATOM
5307
N
GLU
B
113
62.249
48.639
−9.991
1.00
28.56
B
N


ATOM
5308
CA
GLU
B
113
61.517
49.574
−10.801
1.00
29.69
B
C


ATOM
5309
CB
GLU
B
113
60.602
50.447
−9.933
1.00
37.49
B
C


ATOM
5310
CG
GLU
B
113
59.510
51.122
−10.751
1.00
47.65
B
C


ATOM
5311
CD
GLU
B
113
58.598
52.026
−9.935
1.00
69.63
B
C


ATOM
5312
OE1
GLU
B
113
59.086
52.679
−8.989
1.00
75.21
B
O


ATOM
5313
OE2
GLU
B
113
57.387
52.090
−10.254
1.00
70.93
B
O


ATOM
5314
C
GLU
B
113
62.480
50.453
−11.600
1.00
23.60
B
C


ATOM
5315
O
GLU
B
113
63.218
51.264
−11.057
1.00
31.81
B
O


ATOM
5316
N
VAL
B
114
62.474
50.274
−12.908
1.00
20.86
B
N


ATOM
5317
CA
VAL
B
114
63.187
51.163
−13.817
1.00
21.32
B
C


ATOM
5318
CB
VAL
B
114
64.453
50.479
−14.383
1.00
23.76
B
C


ATOM
5319
CG1
VAL
B
114
65.526
50.343
−13.282
1.00
20.01
B
C


ATOM
5320
CG2
VAL
B
114
64.110
49.127
−14.983
1.00
21.05
B
C


ATOM
5321
C
VAL
B
114
62.231
51.605
−14.947
1.00
21.51
B
C


ATOM
5322
O
VAL
B
114
61.294
50.891
−15.332
1.00
20.01
B
O


ATOM
5323
N
SER
B
115
62.474
52.789
−15.465
1.00
17.24
B
N


ATOM
5324
CA
SER
B
115
61.633
53.413
−16.476
1.00
21.29
B
C


ATOM
5325
CB
SER
B
115
61.302
54.859
−16.017
1.00
22.76
B
C


ATOM
5326
OG
SER
B
115
60.931
55.680
−17.115
1.00
27.64
B
O


ATOM
5327
C
SER
B
115
62.354
53.459
−17.824
1.00
21.00
B
C


ATOM
5328
O
SER
B
115
63.582
53.591
−17.885
1.00
17.43
B
O


ATOM
5329
N
GLN
B
116
61.586
53.412
−18.906
1.00
20.95
B
N


ATOM
5330
CA
GLN
B
116
62.156
53.577
−20.255
1.00
25.47
B
C


ATOM
5331
CB
GLN
B
116
61.095
53.316
−21.348
1.00
28.24
B
C


ATOM
5332
CG
GLN
B
116
59.871
54.267
−21.348
1.00
28.04
B
C


ATOM
5333
CD
GLN
B
116
59.077
54.234
−22.640
1.00
27.34
B
C


ATOM
5334
OE1
GLN
B
116
59.359
53.446
−23.535
1.00
29.10
B
O


ATOM
5335
NE2
GLN
B
116
58.079
55.102
−22.741
1.00
22.35
B
N


ATOM
5336
C
GLN
B
116
62.812
54.942
−20.479
1.00
28.20
B
C


ATOM
5337
O
GLN
B
116
63.601
55.104
−21.414
1.00
23.76
B
O


ATOM
5338
N
GLU
B
117
62.497
55.917
−19.622
1.00
31.18
B
N


ATOM
5339
CA
GLU
B
117
63.149
57.240
−19.678
1.00
36.90
B
C


ATOM
5340
CB
GLU
B
117
62.524
58.228
−18.676
1.00
41.23
B
C


ATOM
5341
CG
GLU
B
117
61.061
58.577
−18.936
1.00
43.94
B
C


ATOM
5342
CD
GLU
B
117
60.827
59.150
−20.321
1.00
50.10
B
C


ATOM
5343
OE1
GLU
B
117
61.675
59.929
−20.808
1.00
62.87
B
O


ATOM
5344
OE2
GLU
B
117
59.789
58.817
−20.931
1.00
60.89
B
O


ATOM
5345
C
GLU
B
117
64.659
57.150
−19.434
1.00
30.52
B
C


ATOM
5346
O
GLU
B
117
65.385
58.038
−19.801
1.00
21.21
B
O


ATOM
5347
N
ALA
B
118
65.126
56.052
−18.854
1.00
26.64
B
N


ATOM
5348
CA
ALA
B
118
66.555
55.806
−18.676
1.00
24.31
B
C


ATOM
5349
CB
ALA
B
118
66.763
54.394
−18.052
1.00
23.93
B
C


ATOM
5350
C
ALA
B
118
67.339
55.936
−19.987
1.00
21.81
B
C


ATOM
5351
O
ALA
B
118
68.532
56.230
−19.976
1.00
20.44
B
O


ATOM
5352
N
PHE
B
119
66.680
55.712
−21.116
1.00
23.54
B
N


ATOM
5353
CA
PHE
B
119
67.342
55.831
−22.412
1.00
29.59
B
C


ATOM
5354
CB
PHE
B
119
66.847
54.728
−23.335
1.00
29.29
B
C


ATOM
5355
CG
PHE
B
119
67.164
53.348
−22.833
1.00
27.07
B
C


ATOM
5356
CD1
PHE
B
119
68.415
52.781
−23.076
1.00
26.61
B
C


ATOM
5357
CE1
PHE
B
119
68.721
51.515
−22.614
1.00
24.31
B
C


ATOM
5358
CZ
PHE
B
119
67.785
50.801
−21.891
1.00
23.77
B
C


ATOM
5359
CE2
PHE
B
119
66.539
51.350
−21.624
1.00
28.37
B
C


ATOM
5360
CD2
PHE
B
119
66.233
52.634
−22.099
1.00
26.90
B
C


ATOM
5361
C
PHE
B
119
67.144
57.186
−23.112
1.00
34.14
B
C


ATOM
5362
O
PHE
B
119
67.561
57.340
−24.267
1.00
31.83
B
O


ATOM
5363
N
SER
B
120
66.536
58.164
−22.443
1.00
34.61
B
N


ATOM
5364
CA
ASER
B
120
66.218
59.461
−23.082
0.50
37.13
B
C


ATOM
5365
CA
BSER
B
120
66.216
59.426
−23.131
0.50
40.94
B
C


ATOM
5366
CB
ASER
B
120
65.327
60.320
−22.177
0.50
33.88
B
C


ATOM
5367
CB
BSER
B
120
65.169
60.234
−22.357
0.50
42.91
B
C


ATOM
5368
OG
ASER
B
120
66.010
60.728
−21.007
0.50
24.33
B
O


ATOM
5369
OG
BSER
B
120
63.881
59.640
−22.507
0.50
38.32
B
O


ATOM
5370
C
SER
B
120
67.470
60.252
−23.446
1.00
38.63
B
C


ATOM
5371
O
SER
B
120
67.493
60.979
−24.434
1.00
45.53
B
O


ATOM
5372
N
GLY
B
121
68.515
60.106
−22.637
1.00
38.31
B
N


ATOM
5373
CA
GLY
B
121
69.784
60.780
−22.881
1.00
41.06
B
C


ATOM
5374
C
GLY
B
121
70.494
60.384
−24.169
1.00
41.78
B
C


ATOM
5375
O
GLY
B
121
71.424
61.066
−24.585
1.00
43.25
B
O


ATOM
5376
N
PHE
B
122
70.061
59.297
−24.807
1.00
38.87
B
N


ATOM
5377
CA
PHE
B
122
70.718
58.783
−26.021
1.00
39.39
B
C


ATOM
5378
CB
PHE
B
122
71.017
57.285
−25.843
1.00
38.14
B
C


ATOM
5379
CG
PHE
B
122
71.710
56.979
−24.551
1.00
37.60
B
C


ATOM
5380
CD1
PHE
B
122
73.091
57.030
−24.466
1.00
35.88
B
C


ATOM
5381
CE1
PHE
B
122
73.736
56.783
−23.262
1.00
38.04
B
C


ATOM
5382
CZ
PHE
B
122
72.997
56.494
−22.132
1.00
36.17
B
C


ATOM
5383
CE2
PHE
B
122
71.618
56.448
−22.202
1.00
44.59
B
C


ATOM
5384
CD2
PHE
B
122
70.978
56.692
−23.405
1.00
39.21
B
C


ATOM
5385
C
PHE
B
122
69.937
59.036
−27.320
1.00
36.51
B
C


ATOM
5386
O
PHE
B
122
70.289
58.491
−28.367
1.00
37.34
B
O


ATOM
5387
N
LYS
B
123
68.890
59.857
−27.251
1.00
38.74
B
N


ATOM
5388
CA
LYS
B
123
68.112
60.229
−28.438
1.00
46.15
B
C


ATOM
5389
CB
LYS
B
123
66.612
60.183
−28.125
1.00
51.47
B
C


ATOM
5390
CG
LYS
B
123
66.067
58.761
−27.913
1.00
54.05
B
C


ATOM
5391
CD
LYS
B
123
64.842
58.698
−26.965
1.00
59.06
B
C


ATOM
5392
CE
LYS
B
123
63.518
59.033
−27.656
1.00
55.33
B
C


ATOM
5393
NZ
LYS
B
123
62.999
57.931
−28.521
1.00
52.50
B
N


ATOM
5394
C
LYS
B
123
68.523
61.625
−28.928
1.00
53.18
B
C


ATOM
5395
O
LYS
B
123
68.897
62.486
−28.129
1.00
46.65
B
O


ATOM
5396
N
ASP
B
124
68.469
61.837
−30.243
1.00
54.74
B
N


ATOM
5397
CA
ASP
B
124
68.799
63.145
−30.844
1.00
48.78
B
C


ATOM
5398
CB
ASP
B
124
69.229
62.982
−32.323
1.00
41.62
B
C


ATOM
5399
CG
ASP
B
124
68.121
62.412
−33.219
1.00
39.32
B
C


ATOM
5400
OD1
ASP
B
124
66.927
62.460
−32.844
1.00
36.69
B
O


ATOM
5401
OD2
ASP
B
124
68.448
61.901
−34.309
1.00
43.22
B
O


ATOM
5402
C
ASP
B
124
67.624
64.125
−30.707
1.00
52.38
B
C


ATOM
5403
O
ASP
B
124
66.642
63.834
−30.017
1.00
48.79
B
O


ATOM
5404
N
GLN
B
125
67.736
65.281
−31.364
1.00
54.13
B
N


ATOM
5405
CA
GLN
B
125
66.648
66.268
−31.441
1.00
58.11
B
C


ATOM
5406
CB
GLN
B
125
67.048
67.430
−32.342
1.00
57.23
B
C


ATOM
5407
CG
GLN
B
125
68.219
68.223
−31.829
1.00
66.12
B
C


ATOM
5408
CD
GLN
B
125
68.351
69.568
−32.513
1.00
75.22
B
C


ATOM
5409
OE1
GLN
B
125
69.145
70.404
−32.095
1.00
91.78
B
O


ATOM
5410
NE2
GLN
B
125
67.568
69.786
−33.569
1.00
72.21
B
N


ATOM
5411
C
GLN
B
125
65.342
65.694
−31.983
1.00
58.06
B
C


ATOM
5412
O
GLN
B
125
64.274
65.920
−31.412
1.00
50.39
B
O


ATOM
5413
N
ASN
B
126
65.432
64.959
−33.091
1.00
53.50
B
N


ATOM
5414
CA
ASN
B
126
64.255
64.335
−33.701
1.00
54.83
B
C


ATOM
5415
CB
ASN
B
126
64.621
63.686
−35.039
1.00
48.62
B
C


ATOM
5416
CG
ASN
B
126
64.985
64.701
−36.090
1.00
48.04
B
C


ATOM
5417
OD1
ASN
B
126
65.360
65.831
−35.776
1.00
37.87
B
O


ATOM
5418
ND2
ASN
B
126
64.885
64.305
−37.352
1.00
53.77
B
N


ATOM
5419
C
ASN
B
126
63.556
63.302
−32.806
1.00
55.85
B
C


ATOM
5420
O
ASN
B
126
62.418
62.921
−33.082
1.00
51.34
B
O


ATOM
5421
N
GLY
B
127
64.230
62.867
−31.740
1.00
50.88
B
N


ATOM
5422
CA
GLY
B
127
63.692
61.855
−30.828
1.00
53.73
B
C


ATOM
5423
C
GLY
B
127
64.049
60.439
−31.243
1.00
47.40
B
C


ATOM
5424
O
GLY
B
127
63.411
59.484
−30.809
1.00
50.16
B
O


ATOM
5425
N
ASN
B
128
65.065
60.319
−32.093
1.00
40.27
B
N


ATOM
5426
CA
ASN
B
128
65.569
59.042
−32.568
1.00
45.14
B
C


ATOM
5427
CB
ASN
B
128
65.719
59.037
−34.094
1.00
44.88
B
C


ATOM
5428
CG
ASN
B
128
64.480
59.550
−34.806
1.00
48.63
B
C


ATOM
5429
OD1
ASN
B
128
64.569
60.360
−35.733
1.00
49.05
B
O


ATOM
5430
ND2
ASN
B
128
63.317
59.094
−34.366
1.00
49.33
B
N


ATOM
5431
C
ASN
B
128
66.922
58.801
−31.926
1.00
46.12
B
C


ATOM
5432
O
ASN
B
128
67.652
59.744
−31.626
1.00
49.12
B
O


ATOM
5433
N
PHE
B
129
67.250
57.536
−31.707
1.00
42.63
B
N


ATOM
5434
CA
PHE
B
129
68.544
57.172
−31.137
1.00
42.17
B
C


ATOM
5435
CB
PHE
B
129
68.658
55.648
−31.006
1.00
38.64
B
C


ATOM
5436
CG
PHE
B
129
67.878
55.080
−29.837
1.00
36.83
B
C


ATOM
5437
CD1
PHE
B
129
68.415
55.106
−28.557
1.00
33.99
B
C


ATOM
5438
CE1
PHE
B
129
67.709
54.596
−27.477
1.00
36.82
B
C


ATOM
5439
CZ
PHE
B
129
66.453
54.061
−27.670
1.00
33.55
B
C


ATOM
5440
CE2
PHE
B
129
65.895
54.033
−28.942
1.00
34.20
B
C


ATOM
5441
CD2
PHE
B
129
66.608
54.536
−30.018
1.00
33.13
B
C


ATOM
5442
C
PHE
B
129
69.688
57.729
−31.985
1.00
37.32
B
C


ATOM
5443
O
PHE
B
129
69.673
57.598
−33.209
1.00
36.11
B
O


ATOM
5444
N
LEU
B
130
70.662
58.353
−31.329
1.00
35.07
B
N


ATOM
5445
CA
LEU
B
130
71.816
58.939
−32.024
1.00
40.07
B
C


ATOM
5446
CB
LEU
B
130
72.803
59.533
−31.008
1.00
40.58
B
C


ATOM
5447
CG
LEU
B
130
72.306
60.765
−30.227
1.00
39.82
B
C


ATOM
5448
CD1
LEU
B
130
72.966
60.870
−28.861
1.00
36.33
B
C


ATOM
5449
CD2
LEU
B
130
72.531
62.060
−31.028
1.00
35.72
B
C


ATOM
5450
C
LEU
B
130
72.494
57.876
−32.902
1.00
39.82
B
C


ATOM
5451
O
LEU
B
130
72.670
56.735
−32.480
1.00
32.74
B
O


ATOM
5452
N
GLU
B
131
72.856
58.251
−34.124
1.00
37.28
B
N


ATOM
5453
CA
GLU
B
131
73.355
57.301
−35.125
1.00
40.60
B
C


ATOM
5454
CB
GLU
B
131
73.428
57.974
−36.507
1.00
47.10
B
C


ATOM
5455
CG
GLU
B
131
73.804
57.043
−37.674
1.00
52.91
B
C


ATOM
5456
CD
GLU
B
131
72.682
56.082
−38.070
1.00
56.45
B
C


ATOM
5457
OE1
GLU
B
131
71.773
55.832
−37.250
1.00
74.91
B
O


ATOM
5458
OE2
GLU
B
131
72.703
55.569
−39.210
1.00
54.24
B
O


ATOM
5459
C
GLU
B
131
74.725
56.728
−34.782
1.00
39.38
B
C


ATOM
5460
O
GLU
B
131
74.966
55.545
−35.003
1.00
40.85
B
O


ATOM
5461
N
ASN
B
132
75.614
57.569
−34.259
1.00
36.12
B
N


ATOM
5462
CA
ASN
B
132
76.977
57.148
−33.891
1.00
42.27
B
C


ATOM
5463
CB
ASN
B
132
77.784
58.352
−33.406
1.00
46.73
B
C


ATOM
5464
CG
ASN
B
132
77.083
59.117
−32.292
1.00
48.09
B
C


ATOM
5465
OD1
ASN
B
132
76.466
58.533
−31.405
1.00
50.95
B
O


ATOM
5466
ND2
ASN
B
132
77.176
60.432
−32.341
1.00
50.02
B
N


ATOM
5467
C
ASN
B
132
77.067
56.024
−32.834
1.00
41.78
B
C


ATOM
5468
O
ASN
B
132
78.110
55.389
−32.682
1.00
37.33
B
O


ATOM
5469
N
LEU
B
133
75.983
55.797
−32.096
1.00
38.52
B
N


ATOM
5470
CA
LEU
B
133
75.903
54.664
−31.154
1.00
38.43
B
C


ATOM
5471
CB
LEU
B
133
74.553
54.670
−30.433
1.00
34.51
B
C


ATOM
5472
CG
LEU
B
133
74.363
55.868
−29.482
1.00
38.72
B
C


ATOM
5473
CD1
LEU
B
133
72.888
56.026
−29.067
1.00
34.84
B
C


ATOM
5474
CD2
LEU
B
133
75.264
55.742
−28.268
1.00
29.27
B
C


ATOM
5475
C
LEU
B
133
76.134
53.317
−31.857
1.00
34.69
B
C


ATOM
5476
O
LEU
B
133
76.678
52.395
−31.257
1.00
36.37
B
O


ATOM
5477
N
LYS
B
134
75.779
53.234
−33.141
1.00
33.33
B
N


ATOM
5478
CA
LYS
B
134
75.993
52.018
−33.945
1.00
35.81
B
C


ATOM
5479
CB
LYS
B
134
75.509
52.237
−35.386
1.00
37.14
B
C


ATOM
5480
CG
LYS
B
134
76.432
53.141
−36.211
1.00
46.43
B
C


ATOM
5481
CD
LYS
B
134
75.885
53.431
−37.607
1.00
44.56
B
C


ATOM
5482
CE
LYS
B
134
77.004
53.816
−38.580
1.00
41.71
B
C


ATOM
5483
NZ
LYS
B
134
76.650
53.405
−39.957
1.00
41.67
B
N


ATOM
5484
C
LYS
B
134
77.452
51.512
−33.952
1.00
35.23
B
C


ATOM
5485
O
LYS
B
134
77.698
50.337
−34.223
1.00
40.09
B
O


ATOM
5486
N
GLU
B
135
78.404
52.390
−33.650
1.00
32.63
B
N


ATOM
5487
CA
AGLU
B
135
79.821
52.022
−33.698
0.50
36.56
B
C


ATOM
5488
CA
BGLU
B
135
79.829
52.050
−33.676
0.50
36.80
B
C


ATOM
5489
CB
AGLU
B
135
80.703
53.267
−33.858
0.50
37.02
B
C


ATOM
5490
CB
BGLU
B
135
80.674
53.331
−33.756
0.50
37.58
B
C


ATOM
5491
CG
AGLU
B
135
80.304
54.221
−35.015
0.50
37.03
B
C


ATOM
5492
CG
BGLU
B
135
80.422
54.168
−35.027
0.50
38.50
B
C


ATOM
5493
CD
AGLU
B
135
80.492
53.627
−36.407
0.50
38.03
B
C


ATOM
5494
CD
BGLU
B
135
81.151
55.509
−35.032
0.50
39.75
B
C


ATOM
5495
OE1
AGLU
B
135
80.976
52.481
−36.518
0.50
36.37
B
O


ATOM
5496
OE1
BGLU
B
135
81.194
56.191
−33.984
0.50
40.21
B
O


ATOM
5497
OE2
BGLU
B
135
81.672
55.892
−36.099
0.50
41.10
B
O


ATOM
5498
OE2
AGLU
B
135
80.156
54.318
−37.397
0.50
38.97
B
O


ATOM
5499
C
GLU
B
135
80.227
51.222
−32.448
1.00
39.96
B
C


ATOM
5500
O
GLU
B
135
81.279
50.566
−32.431
1.00
34.56
B
O


ATOM
5501
N
ASP
B
136
79.386
51.262
−31.415
1.00
33.09
B
N


ATOM
5502
CA
ASP
B
136
79.635
50.527
−30.173
1.00
31.38
B
C


ATOM
5503
CB
ASP
B
136
79.316
51.423
−28.980
1.00
31.58
B
C


ATOM
5504
CG
ASP
B
136
79.714
50.799
−27.655
1.00
35.43
B
C


ATOM
5505
OD1
ASP
B
136
80.066
49.594
−27.612
1.00
44.94
B
O


ATOM
5506
OD2
ASP
B
136
79.675
51.530
−26.646
1.00
39.28
B
O


ATOM
5507
C
ASP
B
136
78.772
49.262
−30.127
1.00
28.89
B
C


ATOM
5508
O
ASP
B
136
77.660
49.289
−29.610
1.00
26.68
B
O


ATOM
5509
N
ILE
B
137
79.302
48.161
−30.653
1.00
30.70
B
N


ATOM
5510
CA
ILE
B
137
78.533
46.934
−30.846
1.00
26.61
B
C


ATOM
5511
CB
ILE
B
137
79.298
45.911
−31.720
1.00
33.94
B
C


ATOM
5512
CG1
ILE
B
137
79.657
46.515
−33.085
1.00
35.20
B
C


ATOM
5513
CD1
ILE
B
137
78.435
46.944
−33.870
1.00
34.22
B
C


ATOM
5514
CG2
ILE
B
137
78.445
44.688
−31.994
1.00
32.75
B
C


ATOM
5515
C
ILE
B
137
78.104
46.291
−29.514
1.00
28.02
B
C


ATOM
5516
O
ILE
B
137
77.012
45.741
−29.427
1.00
26.31
B
O


ATOM
5517
N
LYS
B
138
78.952
46.374
−28.494
1.00
27.68
B
N


ATOM
5518
CA
LYS
B
138
78.595
45.940
−27.147
1.00
29.84
B
C


ATOM
5519
CB
LYS
B
138
79.701
46.263
−26.146
1.00
34.20
B
C


ATOM
5520
CG
LYS
B
138
80.582
45.100
−25.778
1.00
44.02
B
C


ATOM
5521
CD
LYS
B
138
81.442
45.464
−24.571
1.00
51.03
B
C


ATOM
5522
CE
LYS
B
138
81.980
44.226
−23.880
1.00
58.48
B
C


ATOM
5523
NZ
LYS
B
138
81.557
44.189
−22.456
1.00
57.19
B
N


ATOM
5524
C
LYS
B
138
77.319
46.610
−26.672
1.00
26.53
B
C


ATOM
5525
O
LYS
B
138
76.383
45.942
−26.230
1.00
24.26
B
O


ATOM
5526
N
ALA
B
139
77.294
47.934
−26.775
1.00
26.02
B
N


ATOM
5527
CA
ALA
B
139
76.172
48.719
−26.304
1.00
22.77
B
C


ATOM
5528
CB
ALA
B
139
76.506
50.229
−26.326
1.00
17.44
B
C


ATOM
5529
C
ALA
B
139
74.921
48.429
−27.112
1.00
24.29
B
C


ATOM
5530
O
ALA
B
139
73.821
48.392
−26.547
1.00
22.75
B
O


ATOM
5531
N
ILE
B
140
75.076
48.208
−28.420
1.00
23.37
B
N


ATOM
5532
CA
ILE
B
140
73.914
47.921
−29.284
1.00
22.15
B
C


ATOM
5533
CB
ILE
B
140
74.262
47.983
−30.794
1.00
21.48
B
C


ATOM
5534
CG1
ILE
B
140
74.636
49.407
−31.183
1.00
27.65
B
C


ATOM
5535
CD1
ILE
B
140
73.572
50.458
−30.836
1.00
25.53
B
C


ATOM
5536
CG2
ILE
B
140
73.077
47.542
−31.634
1.00
22.55
B
C


ATOM
5537
C
ILE
B
140
73.305
46.561
−28.957
1.00
20.16
B
C


ATOM
5538
O
ILE
B
140
72.093
46.440
−28.858
1.00
22.67
B
O


ATOM
5539
N
LEU
B
141
74.151
45.549
−28.820
1.00
19.51
B
N


ATOM
5540
CA
LEU
B
141
73.729
44.264
−28.291
1.00
24.91
B
C


ATOM
5541
CB
LEU
B
141
74.928
43.352
−28.058
1.00
27.39
B
C


ATOM
5542
CG
LEU
B
141
75.220
42.377
−29.184
1.00
34.95
B
C


ATOM
5543
CD1
LEU
B
141
76.646
41.835
−29.109
1.00
31.07
B
C


ATOM
5544
CD2
LEU
B
141
74.202
41.262
−29.108
1.00
33.04
B
C


ATOM
5545
C
LEU
B
141
72.975
44.416
−26.977
1.00
20.67
B
C


ATOM
5546
O
LEU
B
141
71.924
43.802
−26.798
1.00
20.92
B
O


ATOM
5547
N
SER
B
142
73.539
45.170
−26.034
1.00
20.17
B
N


ATOM
5548
CA
SER
B
142
72.915
45.332
−24.718
1.00
20.30
B
C


ATOM
5549
CB
SER
B
142
73.777
46.155
−23.768
1.00
23.23
B
C


ATOM
5550
OG
SER
B
142
74.802
45.361
−23.230
1.00
30.11
B
O


ATOM
5551
C
SER
B
142
71.563
45.988
−24.829
1.00
18.07
B
C


ATOM
5552
O
SER
B
142
70.623
45.598
−24.143
1.00
15.63
B
O


ATOM
5553
N
LEU
B
143
71.466
46.976
−25.707
1.00
16.54
B
N


ATOM
5554
CA
LEU
B
143
70.191
47.630
−25.962
1.00
17.71
B
C


ATOM
5555
CB
LEU
B
143
70.407
48.821
−26.885
1.00
18.73
B
C


ATOM
5556
CG
LEU
B
143
69.179
49.672
−27.189
1.00
21.91
B
C


ATOM
5557
CD1
LEU
B
143
68.586
50.367
−25.946
1.00
20.22
B
C


ATOM
5558
CD2
LEU
B
143
69.575
50.688
−28.256
1.00
18.60
B
C


ATOM
5559
C
LEU
B
143
69.147
46.678
−26.558
1.00
17.90
B
C


ATOM
5560
O
LEU
B
143
67.997
46.671
−26.113
1.00
19.28
B
O


ATOM
5561
N
TYR
B
144
69.539
45.899
−27.568
1.00
17.16
B
N


ATOM
5562
CA
TYR
B
144
68.674
44.855
−28.118
1.00
19.80
B
C


ATOM
5563
CB
TYR
B
144
69.422
44.127
−29.224
1.00
19.05
B
C


ATOM
5564
CG
TYR
B
144
68.797
42.874
−29.747
1.00
22.04
B
C


ATOM
5565
CD1
TYR
B
144
67.817
42.912
−30.727
1.00
23.03
B
C


ATOM
5566
CE1
TYR
B
144
67.264
41.752
−31.222
1.00
25.33
B
C


ATOM
5567
CZ
TYR
B
144
67.707
40.533
−30.727
1.00
27.46
B
C


ATOM
5568
OH
TYR
B
144
67.193
39.332
−31.164
1.00
37.98
B
O


ATOM
5569
CE2
TYR
B
144
68.664
40.489
−29.746
1.00
27.56
B
C


ATOM
5570
CD2
TYR
B
144
69.197
41.648
−29.266
1.00
25.78
B
C


ATOM
5571
C
TYR
B
144
68.220
43.876
−27.016
1.00
19.27
B
C


ATOM
5572
O
TYR
B
144
67.031
43.595
−26.893
1.00
16.16
B
O


ATOM
5573
N
GLU
B
145
69.146
43.409
−26.172
1.00
16.42
B
N


ATOM
5574
CA
GLU
B
145
68.756
42.458
−25.120
1.00
16.71
B
C


ATOM
5575
CB
GLU
B
145
69.958
41.934
−24.342
1.00
17.86
B
C


ATOM
5576
CG
GLU
B
145
70.968
41.099
−25.175
1.00
23.74
B
C


ATOM
5577
CD
GLU
B
145
70.433
39.735
−25.637
1.00
27.87
B
C


ATOM
5578
OE1
GLU
B
145
69.241
39.407
−25.369
1.00
29.72
B
O


ATOM
5579
OE2
GLU
B
145
71.222
38.977
−26.262
1.00
22.17
B
O


ATOM
5580
C
GLU
B
145
67.733
43.076
−24.149
1.00
15.36
B
C


ATOM
5581
O
GLU
B
145
66.758
42.433
−23.782
1.00
17.00
B
O


ATOM
5582
N
ALA
B
146
67.953
44.331
−23.772
1.00
15.19
B
N


ATOM
5583
CA
ALA
B
146
67.097
45.031
−22.816
1.00
16.77
B
C


ATOM
5584
CB
ALA
B
146
67.754
46.372
−22.403
1.00
15.90
B
C


ATOM
5585
C
ALA
B
146
65.670
45.259
−23.376
1.00
18.70
B
C


ATOM
5586
O
ALA
B
146
64.674
45.219
−22.630
1.00
16.44
B
O


ATOM
5587
N
SER
B
147
65.575
45.472
−24.684
1.00
17.99
B
N


ATOM
5588
CA
SER
B
147
64.289
45.771
−25.309
1.00
19.54
B
C


ATOM
5589
CB
SER
B
147
64.482
46.091
−26.810
1.00
21.52
B
C


ATOM
5590
OG
SER
B
147
64.753
44.911
−27.566
1.00
22.75
B
O


ATOM
5591
C
SER
B
147
63.224
44.657
−25.108
1.00
18.90
B
C


ATOM
5592
O
SER
B
147
62.018
44.936
−25.070
1.00
20.51
B
O


ATOM
5593
N
PHE
B
148
63.662
43.415
−24.930
1.00
17.55
B
N


ATOM
5594
CA
PHE
B
148
62.754
42.301
−24.758
1.00
18.08
B
C


ATOM
5595
CB
PHE
B
148
63.436
40.969
−25.122
1.00
19.55
B
C


ATOM
5596
CG
PHE
B
148
63.713
40.846
−26.593
1.00
17.56
B
C


ATOM
5597
CD1
PHE
B
148
62.733
40.350
−27.460
1.00
17.90
B
C


ATOM
5598
CE1
PHE
B
148
62.947
40.282
−28.835
1.00
19.84
B
C


ATOM
5599
CZ
PHE
B
148
64.142
40.672
−29.355
1.00
22.18
B
C


ATOM
5600
CE2
PHE
B
148
65.147
41.165
−28.489
1.00
21.23
B
C


ATOM
5601
CD2
PHE
B
148
64.908
41.241
−27.113
1.00
18.18
B
C


ATOM
5602
C
PHE
B
148
62.131
42.286
−23.373
1.00
20.15
B
C


ATOM
5603
O
PHE
B
148
61.179
41.550
−23.139
1.00
16.91
B
O


ATOM
5604
N
LEU
B
149
62.610
43.146
−22.466
1.00
19.45
B
N


ATOM
5605
CA
LEU
B
149
61.991
43.233
−21.136
1.00
17.46
B
C


ATOM
5606
CB
LEU
B
149
63.058
43.450
−20.074
1.00
17.26
B
C


ATOM
5607
CG
LEU
B
149
63.793
42.153
−19.705
1.00
17.95
B
C


ATOM
5608
CD1
LEU
B
149
64.991
41.884
−20.643
1.00
18.08
B
C


ATOM
5609
CD2
LEU
B
149
64.241
42.230
−18.227
1.00
16.84
B
C


ATOM
5610
C
LEU
B
149
60.885
44.267
−21.062
1.00
18.68
B
C


ATOM
5611
O
LEU
B
149
60.398
44.581
−19.969
1.00
16.29
B
O


ATOM
5612
N
ALA
B
150
60.452
44.760
−22.232
1.00
18.92
B
N


ATOM
5613
CA
ALA
B
150
59.486
45.834
−22.293
1.00
21.18
B
C


ATOM
5614
CB
ALA
B
150
59.368
46.350
−23.730
1.00
20.05
B
C


ATOM
5615
C
ALA
B
150
58.108
45.418
−21.764
1.00
21.68
B
C


ATOM
5616
O
ALA
B
150
57.698
44.242
−21.839
1.00
21.33
B
O


ATOM
5617
N
LEU
B
151
57.405
46.408
−21.241
1.00
19.58
B
N


ATOM
5618
CA
LEU
B
151
56.006
46.317
−20.869
1.00
21.35
B
C


ATOM
5619
CB
LEU
B
151
55.788
46.978
−19.504
1.00
23.78
B
C


ATOM
5620
CG
LEU
B
151
56.706
46.538
−18.361
1.00
27.90
B
C


ATOM
5621
CD1
LEU
B
151
56.345
47.224
−17.046
1.00
23.70
B
C


ATOM
5622
CD2
LEU
B
151
56.694
45.043
−18.178
1.00
22.80
B
C


ATOM
5623
C
LEU
B
151
55.156
47.052
−21.920
1.00
20.12
B
C


ATOM
5624
O
LEU
B
151
55.680
47.794
−22.746
1.00
24.87
B
O


ATOM
5625
N
GLU
B
152
53.846
46.847
−21.889
1.00
19.20
B
N


ATOM
5626
CA
GLU
B
152
52.956
47.556
−22.823
1.00
23.41
B
C


ATOM
5627
CB
GLU
B
152
51.509
47.169
−22.577
1.00
29.93
B
C


ATOM
5628
CG
GLU
B
152
51.081
45.890
−23.215
1.00
42.29
B
C


ATOM
5629
CD
GLU
B
152
49.564
45.763
−23.216
1.00
51.02
B
C


ATOM
5630
OE1
GLU
B
152
48.910
46.406
−24.063
1.00
46.01
B
O


ATOM
5631
OE2
GLU
B
152
49.036
45.042
−22.350
1.00
48.21
B
O


ATOM
5632
C
GLU
B
152
53.082
49.065
−22.609
1.00
22.58
B
C


ATOM
5633
O
GLU
B
152
53.146
49.515
−21.465
1.00
21.70
B
O


ATOM
5634
N
GLY
B
153
53.146
49.822
−23.695
1.00
24.58
B
N


ATOM
5635
CA
GLY
B
153
53.226
51.285
−23.635
1.00
24.61
B
C


ATOM
5636
C
GLY
B
153
54.660
51.812
−23.622
1.00
27.73
B
C


ATOM
5637
O
GLY
B
153
54.876
53.011
−23.701
1.00
26.02
B
O


ATOM
5638
N
GLU
B
154
55.657
50.929
−23.517
1.00
23.81
B
N


ATOM
5639
CA
GLU
B
154
57.041
51.368
−23.509
1.00
22.75
B
C


ATOM
5640
CB
GLU
B
154
57.900
50.488
−22.600
1.00
27.37
B
C


ATOM
5641
CG
GLU
B
154
57.580
50.713
−21.102
1.00
27.77
B
C


ATOM
5642
CD
GLU
B
154
58.439
49.874
−20.166
1.00
28.06
B
C


ATOM
5643
OE1
GLU
B
154
58.954
48.821
−20.597
1.00
28.68
B
O


ATOM
5644
OE2
GLU
B
154
58.601
50.253
−18.984
1.00
27.35
B
O


ATOM
5645
C
GLU
B
154
57.555
51.450
−24.949
1.00
26.56
B
C


ATOM
5646
O
GLU
B
154
58.352
50.647
−25.410
1.00
19.20
B
O


ATOM
5647
N
ASN
B
155
57.086
52.480
−25.648
1.00
30.84
B
N


ATOM
5648
CA
ASN
B
155
57.417
52.689
−27.050
1.00
25.67
B
C


ATOM
5649
CB
ASN
B
155
56.641
53.907
−27.574
1.00
28.27
B
C


ATOM
5650
CG
ASN
B
155
56.924
55.162
−26.758
1.00
32.90
B
C


ATOM
5651
OD1
ASN
B
155
56.439
55.307
−25.636
1.00
31.26
B
O


ATOM
5652
ND2
ASN
B
155
57.741
56.055
−27.303
1.00
36.59
B
N


ATOM
5653
C
ASN
B
155
58.913
52.902
−27.304
1.00
23.66
B
C


ATOM
5654
O
ASN
B
155
59.417
52.539
−28.365
1.00
27.16
B
O


ATOM
5655
N
ILE
B
156
59.626
53.500
−26.351
1.00
22.77
B
N


ATOM
5656
CA
ILE
B
156
61.063
53.706
−26.506
1.00
21.62
B
C


ATOM
5657
CB
ILE
B
156
61.690
54.544
−25.351
1.00
27.45
B
C


ATOM
5658
CG1
ILE
B
156
61.075
55.949
−25.305
1.00
27.27
B
C


ATOM
5659
CD1
ILE
B
156
61.899
56.951
−24.487
1.00
26.26
B
C


ATOM
5660
CG2
ILE
B
156
63.182
54.702
−25.563
1.00
22.98
B
C


ATOM
5661
C
ILE
B
156
61.805
52.384
−26.634
1.00
22.91
B
C


ATOM
5662
O
ILE
B
156
62.715
52.268
−27.456
1.00
24.66
B
O


ATOM
5663
N
LEU
B
157
61.409
51.382
−25.843
1.00
24.03
B
N


ATOM
5664
CA
LEU
B
157
62.023
50.062
−25.927
1.00
24.42
B
C


ATOM
5665
CB
LEU
B
157
61.569
49.153
−24.770
1.00
21.56
B
C


ATOM
5666
CG
LEU
B
157
61.977
49.634
−23.378
1.00
26.08
B
C


ATOM
5667
CD1
LEU
B
157
61.751
48.552
−22.330
1.00
27.55
B
C


ATOM
5668
CD2
LEU
B
157
63.413
50.040
−23.385
1.00
26.42
B
C


ATOM
5669
C
LEU
B
157
61.722
49.397
−27.254
1.00
23.32
B
C


ATOM
5670
O
LEU
B
157
62.597
48.762
−27.832
1.00
23.85
B
O


ATOM
5671
N
ASP
B
158
60.492
49.545
−27.740
1.00
22.27
B
N


ATOM
5672
CA
ASP
B
158
60.148
49.059
−29.075
1.00
25.95
B
C


ATOM
5673
CB
ASP
B
158
58.651
49.287
−29.352
1.00
27.41
B
C


ATOM
5674
CG
ASP
B
158
57.754
48.257
−28.632
1.00
41.98
B
C


ATOM
5675
OD1
ASP
B
158
58.256
47.220
−28.150
1.00
46.41
B
O


ATOM
5676
OD2
ASP
B
158
56.535
48.476
−28.544
1.00
45.63
B
O


ATOM
5677
C
ASP
B
158
61.017
49.710
−30.155
1.00
22.57
B
C


ATOM
5678
O
ASP
B
158
61.518
49.045
−31.062
1.00
22.80
B
O


ATOM
5679
N
GLU
B
159
61.216
51.007
−30.022
1.00
25.58
B
N


ATOM
5680
CA
GLU
B
159
62.062
51.764
−30.941
1.00
29.22
B
C


ATOM
5681
CB
GLU
B
159
61.980
53.261
−30.619
1.00
33.36
B
C


ATOM
5682
CG
GLU
B
159
60.610
53.898
−30.909
1.00
42.11
B
C


ATOM
5683
CD
GLU
B
159
60.382
55.249
−30.206
1.00
43.81
B
C


ATOM
5684
OE1
GLU
B
159
61.347
56.017
−29.991
1.00
48.43
B
O


ATOM
5685
OE2
GLU
B
159
59.213
55.547
−29.882
1.00
51.64
B
O


ATOM
5686
C
GLU
B
159
63.513
51.275
−30.836
1.00
26.50
B
C


ATOM
5687
O
GLU
B
159
64.193
51.124
−31.843
1.00
22.26
B
O


ATOM
5688
N
ALA
B
160
63.971
51.011
−29.614
1.00
22.74
B
N


ATOM
5689
CA
ALA
B
160
65.339
50.514
−29.408
1.00
25.08
B
C


ATOM
5690
CB
ALA
B
160
65.658
50.332
−27.919
1.00
26.19
B
C


ATOM
5691
C
ALA
B
160
65.571
49.214
−30.143
1.00
25.21
B
C


ATOM
5692
O
ALA
B
160
66.610
49.051
−30.778
1.00
24.90
B
O


ATOM
5693
N
LYS
B
161
64.607
48.295
−30.060
1.00
22.51
B
N


ATOM
5694
CA
LYS
B
161
64.740
47.011
−30.734
1.00
24.00
B
C


ATOM
5695
CB
LYS
B
161
63.550
46.091
−30.422
1.00
24.85
B
C


ATOM
5696
CG
LYS
B
161
63.715
44.676
−30.956
1.00
25.57
B
C


ATOM
5697
CD
LYS
B
161
62.422
43.873
−30.927
1.00
30.04
B
C


ATOM
5698
CE
LYS
B
161
61.946
43.588
−29.482
1.00
30.85
B
C


ATOM
5699
NZ
LYS
B
161
60.562
42.972
−29.451
1.00
32.20
B
N


ATOM
5700
C
LYS
B
161
64.884
47.200
−32.245
1.00
21.81
B
C


ATOM
5701
O
LYS
B
161
65.754
46.590
−32.866
1.00
21.50
B
O


ATOM
5702
N
VAL
B
162
64.036
48.052
−32.813
1.00
23.96
B
N


ATOM
5703
CA
VAL
B
162
64.039
48.303
−34.258
1.00
24.15
B
C


ATOM
5704
CB
VAL
B
162
62.855
49.245
−34.678
1.00
22.34
B
C


ATOM
5705
CG1
VAL
B
162
63.017
49.744
−36.132
1.00
21.25
B
C


ATOM
5706
CG2
VAL
B
162
61.506
48.522
−34.475
1.00
21.74
B
C


ATOM
5707
C
VAL
B
162
65.395
48.884
−34.642
1.00
25.05
B
C


ATOM
5708
O
VAL
B
162
66.068
48.377
−35.545
1.00
26.49
B
O


ATOM
5709
N
PHE
B
163
65.804
49.924
−33.923
1.00
24.34
B
N


ATOM
5710
CA
PHE
B
163
67.139
50.515
−34.082
1.00
24.16
B
C


ATOM
5711
CB
PHE
B
163
67.339
51.619
−33.040
1.00
24.78
B
C


ATOM
5712
CG
PHE
B
163
68.696
52.250
−33.061
1.00
24.62
B
C


ATOM
5713
CD1
PHE
B
163
69.011
53.217
−34.004
1.00
31.24
B
C


ATOM
5714
CE1
PHE
B
163
70.252
53.814
−34.016
1.00
29.93
B
C


ATOM
5715
CZ
PHE
B
163
71.214
53.454
−33.065
1.00
32.86
B
C


ATOM
5716
CE2
PHE
B
163
70.919
52.494
−32.121
1.00
31.64
B
C


ATOM
5717
CD2
PHE
B
163
69.655
51.901
−32.115
1.00
29.49
B
C


ATOM
5718
C
PHE
B
163
68.271
49.495
−33.981
1.00
26.28
B
C


ATOM
5719
O
PHE
B
163
69.100
49.391
−34.889
1.00
24.95
B
O


ATOM
5720
N
ALA
B
164
68.320
48.751
−32.876
1.00
25.01
B
N


ATOM
5721
CA
ALA
B
164
69.425
47.832
−32.638
1.00
21.33
B
C


ATOM
5722
CB
ALA
B
164
69.322
47.173
−31.206
1.00
21.86
B
C


ATOM
5723
C
ALA
B
164
69.504
46.751
−33.704
1.00
19.03
B
C


ATOM
5724
O
ALA
B
164
70.586
46.467
−34.213
1.00
21.68
B
O


ATOM
5725
N
ILE
B
165
68.373
46.131
−34.026
1.00
21.18
B
N


ATOM
5726
CA
ILE
B
165
68.323
45.060
−35.045
1.00
25.74
B
C


ATOM
5727
CB
ILE
B
165
66.890
44.534
−35.246
1.00
32.13
B
C


ATOM
5728
CG1
ILE
B
165
66.488
43.594
−34.114
1.00
37.00
B
C


ATOM
5729
CD1
ILE
B
165
64.986
43.262
−34.103
1.00
31.74
B
C


ATOM
5730
CG2
ILE
B
165
66.749
43.785
−36.570
1.00
36.42
B
C


ATOM
5731
C
ILE
B
165
68.871
45.550
−36.405
1.00
28.32
B
C


ATOM
5732
O
ILE
B
165
69.575
44.812
−37.100
1.00
25.65
B
O


ATOM
5733
N
SER
B
166
68.572
46.800
−36.757
1.00
31.22
B
N


ATOM
5734
CA
ASER
B
166
69.023
47.393
−38.027
0.50
31.91
B
C


ATOM
5735
CA
BSER
B
166
69.022
47.367
−38.033
0.50
33.95
B
C


ATOM
5736
CB
ASER
B
166
68.533
48.843
−38.167
0.50
27.64
B
C


ATOM
5737
CB
BSER
B
166
68.491
48.787
−38.213
0.50
31.99
B
C


ATOM
5738
OG
ASER
B
166
69.361
49.750
−37.451
0.50
23.79
B
O


ATOM
5739
OG
BSER
B
166
67.081
48.793
−38.137
0.50
32.33
B
O


ATOM
5740
C
SER
B
166
70.539
47.374
−38.154
1.00
33.66
B
C


ATOM
5741
O
SER
B
166
71.077
47.051
−39.205
1.00
34.05
B
O


ATOM
5742
N
HIS
B
167
71.223
47.729
−37.074
1.00
33.02
B
N


ATOM
5743
CA
HIS
B
167
72.675
47.835
−37.121
1.00
34.21
B
C


ATOM
5744
CB
HIS
B
167
73.151
48.973
−36.214
1.00
33.91
B
C


ATOM
5745
CG
HIS
B
167
72.612
50.339
−36.628
1.00
45.20
B
C


ATOM
5746
ND1
HIS
B
167
72.967
50.938
−37.785
1.00
48.79
B
N


ATOM
5747
CE1
HIS
B
167
72.322
52.119
−37.898
1.00
47.04
B
C


ATOM
5748
NE2
HIS
B
167
71.547
52.277
−36.812
1.00
50.51
B
N


ATOM
5749
CD2
HIS
B
167
71.697
51.197
−36.009
1.00
48.48
B
C


ATOM
5750
C
HIS
B
167
73.355
46.525
−36.836
1.00
34.42
B
C


ATOM
5751
O
HIS
B
167
74.508
46.339
−37.218
1.00
37.01
B
O


ATOM
5752
N
LEU
B
168
72.646
45.588
−36.204
1.00
32.96
B
N


ATOM
5753
CA
LEU
B
168
73.216
44.270
−35.896
1.00
28.12
B
C


ATOM
5754
CB
LEU
B
168
72.470
43.593
−34.725
1.00
25.45
B
C


ATOM
5755
CG
LEU
B
168
72.682
44.212
−33.331
1.00
26.35
B
C


ATOM
5756
CD1
LEU
B
168
71.704
43.639
−32.309
1.00
26.86
B
C


ATOM
5757
CD2
LEU
B
168
74.113
44.036
−32.855
1.00
21.75
B
C


ATOM
5758
C
LEU
B
168
73.262
43.328
−37.108
1.00
32.22
B
C


ATOM
5759
O
LEU
B
168
74.273
42.653
−37.329
1.00
36.39
B
O


ATOM
5760
N
LYS
B
169
72.191
43.259
−37.889
1.00
34.18
B
N


ATOM
5761
CA
LYS
B
169
72.147
42.236
−38.950
1.00
48.30
B
C


ATOM
5762
CB
LYS
B
169
70.714
41.853
−39.357
1.00
53.20
B
C


ATOM
5763
CG
LYS
B
169
69.813
42.939
−39.906
1.00
58.88
B
C


ATOM
5764
CD
LYS
B
169
68.360
42.442
−39.832
1.00
68.90
B
C


ATOM
5765
CE
LYS
B
169
67.344
43.516
−40.162
1.00
70.33
B
C


ATOM
5766
NZ
LYS
B
169
67.345
43.859
−41.605
1.00
68.49
B
N


ATOM
5767
C
LYS
B
169
73.025
42.535
−40.167
1.00
45.13
B
C


ATOM
5768
O
LYS
B
169
73.391
41.630
−40.912
1.00
43.68
B
O


ATOM
5769
N
GLU
B
170
73.417
43.783
−40.342
1.00
42.63
B
N


ATOM
5770
CA
GLU
B
170
74.257
44.114
−41.484
1.00
56.02
B
C


ATOM
5771
CB
GLU
B
170
73.871
45.483
−42.039
1.00
60.46
B
C


ATOM
5772
CG
GLU
B
170
74.152
46.645
−41.096
1.00
69.20
B
C


ATOM
5773
CD
GLU
B
170
73.483
47.941
−41.527
1.00
77.95
B
C


ATOM
5774
OE1
GLU
B
170
72.775
47.954
−42.559
1.00
83.41
B
O


ATOM
5775
OE2
GLU
B
170
73.667
48.955
−40.823
1.00
76.71
B
O


ATOM
5776
C
GLU
B
170
75.745
44.062
−41.126
1.00
54.62
B
C


ATOM
5777
O
GLU
B
170
76.570
44.610
−41.852
1.00
59.09
B
O


ATOM
5778
N
LEU
B
171
76.089
43.398
−40.023
1.00
52.49
B
N


ATOM
5779
CA
LEU
B
171
77.473
43.384
−39.535
1.00
48.96
B
C


ATOM
5780
CB
LEU
B
171
77.527
43.246
−38.012
1.00
47.66
B
C


ATOM
5781
CG
LEU
B
171
77.149
44.474
−37.190
1.00
50.18
B
C


ATOM
5782
CD1
LEU
B
171
77.310
44.165
−35.705
1.00
52.90
B
C


ATOM
5783
CD2
LEU
B
171
77.975
45.689
−37.577
1.00
40.88
B
C


ATOM
5784
C
LEU
B
171
78.299
42.265
−40.151
1.00
50.12
B
C


ATOM
5785
O
LEU
B
171
77.885
41.107
−40.152
1.00
47.31
B
O


ATOM
5786
N
SER
B
172
79.484
42.632
−40.640
1.00
50.94
B
N


ATOM
5787
CA
SER
B
172
80.434
41.693
−41.222
1.00
51.81
B
C


ATOM
5788
CB
SER
B
172
81.237
42.397
−42.327
1.00
55.69
B
C


ATOM
5789
OG
SER
B
172
82.034
41.484
−43.058
1.00
57.01
B
O


ATOM
5790
C
SER
B
172
81.387
41.182
−40.146
1.00
42.54
B
C


ATOM
5791
O
SER
B
172
81.985
41.983
−39.419
1.00
44.20
B
O


ATOM
5792
N
GLU
B
173
81.533
39.858
−40.048
1.00
42.14
B
N


ATOM
5793
CA
GLU
B
173
82.523
39.245
−39.148
1.00
49.56
B
C


ATOM
5794
CB
GLU
B
173
82.579
37.724
−39.349
1.00
58.65
B
C


ATOM
5795
CG
GLU
B
173
83.863
37.069
−38.808
1.00
69.97
B
C


ATOM
5796
CD
GLU
B
173
83.738
35.574
−38.576
1.00
74.14
B
C


ATOM
5797
OE1
GLU
B
173
82.704
34.977
−38.947
1.00
77.61
B
O


ATOM
5798
OE2
GLU
B
173
84.688
34.994
−38.011
1.00
83.80
B
O


ATOM
5799
C
GLU
B
173
83.909
39.851
−39.367
1.00
51.07
B
C


ATOM
5800
O
GLU
B
173
84.649
40.113
−38.410
1.00
40.49
B
O


ATOM
5801
N
GLU
B
174
84.241
40.048
−40.642
1.00
59.07
B
N


ATOM
5802
CA
GLU
B
174
85.445
40.753
−41.054
1.00
57.26
B
C


ATOM
5803
CB
GLU
B
174
85.308
41.184
−42.514
1.00
70.84
B
C


ATOM
5804
CG
GLU
B
174
85.534
40.070
−43.512
1.00
75.42
B
C


ATOM
5805
CD
GLU
B
174
87.006
39.780
−43.695
1.00
76.79
B
C


ATOM
5806
OE1
GLU
B
174
87.636
40.410
−44.571
1.00
71.71
B
O


ATOM
5807
OE2
GLU
B
174
87.536
38.931
−42.949
1.00
74.53
B
O


ATOM
5808
C
GLU
B
174
85.696
41.986
−40.202
1.00
55.82
B
C


ATOM
5809
O
GLU
B
174
86.756
42.128
−39.589
1.00
53.13
B
O


ATOM
5810
N
LYS
B
175
84.703
42.865
−40.155
1.00
59.43
B
N


ATOM
5811
CA
LYS
B
175
84.865
44.163
−39.511
1.00
59.17
B
C


ATOM
5812
CB
LYS
B
175
83.782
45.132
−39.989
1.00
67.78
B
C


ATOM
5813
CG
LYS
B
175
83.866
45.355
−41.489
1.00
79.99
B
C


ATOM
5814
CD
LYS
B
175
82.941
46.430
−42.009
1.00
87.78
B
C


ATOM
5815
CE
LYS
B
175
83.024
46.483
−43.530
1.00
79.47
B
C


ATOM
5816
NZ
LYS
B
175
82.498
47.758
−44.087
1.00
82.96
B
N


ATOM
5817
C
LYS
B
175
84.898
44.074
−37.990
1.00
52.80
B
C


ATOM
5818
O
LYS
B
175
85.698
44.756
−37.354
1.00
46.32
B
O


ATOM
5819
N
ILE
B
176
84.065
43.216
−37.403
1.00
47.03
B
N


ATOM
5820
CA
ILE
B
176
83.947
43.172
−35.934
1.00
41.39
B
C


ATOM
5821
CB
ILE
B
176
82.467
43.162
−35.483
1.00
37.31
B
C


ATOM
5822
CG1
ILE
B
176
81.765
41.858
−35.896
1.00
40.17
B
C


ATOM
5823
CD1
ILE
B
176
80.314
41.783
−35.465
1.00
36.72
B
C


ATOM
5824
CG2
ILE
B
176
81.740
44.395
−36.034
1.00
32.47
B
C


ATOM
5825
C
ILE
B
176
84.721
42.042
−35.233
1.00
38.72
B
C


ATOM
5826
O
ILE
B
176
84.978
42.124
−34.029
1.00
36.23
B
O


ATOM
5827
N
GLY
B
177
85.083
40.996
−35.974
1.00
37.16
B
N


ATOM
5828
CA
GLY
B
177
85.806
39.860
−35.401
1.00
38.29
B
C


ATOM
5829
C
GLY
B
177
84.880
38.695
−35.094
1.00
44.77
B
C


ATOM
5830
O
GLY
B
177
83.673
38.878
−34.937
1.00
41.96
B
O


ATOM
5831
N
LYS
B
178
85.468
37.504
−34.985
1.00
43.62
B
N


ATOM
5832
CA
LYS
B
178
84.735
36.235
−34.868
1.00
41.16
B
C


ATOM
5833
CB
LYS
B
178
85.733
35.073
−34.955
1.00
45.58
B
C


ATOM
5834
CG
LYS
B
178
85.133
33.675
−34.886
1.00
52.04
B
C


ATOM
5835
CD
LYS
B
178
86.235
32.622
−34.939
1.00
60.26
B
C


ATOM
5836
CE
LYS
B
178
85.712
31.238
−34.594
1.00
65.99
B
C


ATOM
5837
NZ
LYS
B
178
84.777
30.719
−35.620
1.00
68.96
B
N


ATOM
5838
C
LYS
B
178
83.910
36.127
−33.578
1.00
33.31
B
C


ATOM
5839
O
LYS
B
178
82.724
35.832
−33.619
1.00
33.33
B
O


ATOM
5840
N
GLU
B
179
84.563
36.366
−32.447
1.00
35.34
B
N


ATOM
5841
CA
GLU
B
179
83.946
36.349
−31.121
1.00
42.45
B
C


ATOM
5842
CB
GLU
B
179
85.001
36.767
−30.100
1.00
45.16
B
C


ATOM
5843
CG
GLU
B
179
84.500
37.192
−28.740
1.00
57.61
B
C


ATOM
5844
CD
GLU
B
179
85.651
37.498
−27.792
1.00
71.05
B
C


ATOM
5845
OE1
GLU
B
179
86.814
37.166
−28.129
1.00
62.84
B
O


ATOM
5846
OE2
GLU
B
179
85.398
38.077
−26.713
1.00
92.44
B
O


ATOM
5847
C
GLU
B
179
82.705
37.247
−31.028
1.00
43.58
B
C


ATOM
5848
O
GLU
B
179
81.631
36.815
−30.558
1.00
34.19
B
O


ATOM
5849
N
LEU
B
180
82.846
38.483
−31.497
1.00
36.50
B
N


ATOM
5850
CA
LEU
B
180
81.737
39.417
−31.473
1.00
35.40
B
C


ATOM
5851
CB
LEU
B
180
82.231
40.848
−31.626
1.00
38.16
B
C


ATOM
5852
CG
LEU
B
180
81.318
41.863
−30.940
1.00
40.07
B
C


ATOM
5853
CD1
LEU
B
180
81.242
41.604
−29.421
1.00
37.91
B
C


ATOM
5854
CD2
LEU
B
180
81.786
43.286
−31.253
1.00
37.86
B
C


ATOM
5855
C
LEU
B
180
80.645
39.067
−32.508
1.00
37.86
B
C


ATOM
5856
O
LEU
B
180
79.455
39.223
−32.224
1.00
34.14
B
O


ATOM
5857
N
ALA
B
181
81.027
38.554
−33.677
1.00
38.02
B
N


ATOM
5858
CA
ALA
B
181
80.029
38.064
−34.650
1.00
30.09
B
C


ATOM
5859
CB
ALA
B
181
80.692
37.689
−35.963
1.00
29.54
B
C


ATOM
5860
C
ALA
B
181
79.244
36.869
−34.099
1.00
28.27
B
C


ATOM
5861
O
ALA
B
181
78.053
36.704
−34.385
1.00
23.95
B
O


ATOM
5862
N
GLU
B
182
79.908
36.013
−33.331
1.00
27.80
B
N


ATOM
5863
CA
GLU
B
182
79.213
34.871
−32.740
1.00
31.93
B
C


ATOM
5864
CB
GLU
B
182
80.199
33.842
−32.161
1.00
37.75
B
C


ATOM
5865
CG
GLU
B
182
80.576
32.728
−33.159
1.00
46.68
B
C


ATOM
5866
CD
GLU
B
182
82.013
32.202
−32.999
1.00
56.06
B
C


ATOM
5867
OE1
GLU
B
182
82.650
32.451
−31.950
1.00
57.60
B
O


ATOM
5868
OE2
GLU
B
182
82.510
31.538
−33.937
1.00
62.66
B
O


ATOM
5869
C
GLU
B
182
78.214
35.371
−31.687
1.00
25.88
B
C


ATOM
5870
O
GLU
B
182
77.116
34.850
−31.595
1.00
26.72
B
O


ATOM
5871
N
GLN
B
183
78.590
36.398
−30.925
1.00
24.20
B
N


ATOM
5872
CA
GLN
B
183
77.686
36.984
−29.935
1.00
22.87
B
C


ATOM
5873
CB
GLN
B
183
78.387
38.040
−29.052
1.00
23.49
B
C


ATOM
5874
CG
GLN
B
183
79.385
37.484
−27.988
1.00
25.76
B
C


ATOM
5875
CD
GLN
B
183
78.718
36.735
−26.796
1.00
36.22
B
C


ATOM
5876
OE1
GLN
B
183
77.488
36.781
−26.581
1.00
39.28
B
O


ATOM
5877
NE2
GLN
B
183
79.547
36.040
−26.010
1.00
27.88
B
N


ATOM
5878
C
GLN
B
183
76.462
37.581
−30.641
1.00
20.79
B
C


ATOM
5879
O
GLN
B
183
75.328
37.342
−30.231
1.00
18.96
B
O


ATOM
5880
N
VAL
B
184
76.699
38.317
−31.723
1.00
18.41
B
N


ATOM
5881
CA
VAL
B
184
75.615
38.956
−32.459
1.00
22.44
B
C


ATOM
5882
CB
VAL
B
184
76.134
39.908
−33.560
1.00
21.83
B
C


ATOM
5883
CG1
VAL
B
184
74.959
40.418
−34.416
1.00
25.83
B
C


ATOM
5884
CG2
VAL
B
184
76.922
41.070
−32.924
1.00
21.84
B
C


ATOM
5885
C
VAL
B
184
74.690
37.916
−33.059
1.00
22.10
B
C


ATOM
5886
O
VAL
B
184
73.473
38.058
−32.971
1.00
27.26
B
O


ATOM
5887
N
ASN
B
185
75.256
36.858
−33.646
1.00
24.42
B
N


ATOM
5888
CA
ASN
B
185
74.431
35.789
−34.243
1.00
22.70
B
C


ATOM
5889
CB
ASN
B
185
75.286
34.822
−35.055
1.00
25.47
B
C


ATOM
5890
CG
ASN
B
185
75.910
35.487
−36.292
1.00
33.07
B
C


ATOM
5891
OD1
ASN
B
185
75.334
36.406
−36.882
1.00
34.50
B
O


ATOM
5892
ND2
ASN
B
185
77.112
35.044
−36.655
1.00
27.32
B
N


ATOM
5893
C
ASN
B
185
73.624
35.022
−33.207
1.00
22.45
B
C


ATOM
5894
O
ASN
B
185
72.478
34.684
−33.438
1.00
21.71
B
O


ATOM
5895
N
HIS
B
186
74.215
34.770
−32.050
1.00
19.36
B
N


ATOM
5896
CA
HIS
B
186
73.499
34.086
−30.992
1.00
21.89
B
C


ATOM
5897
CB
HIS
B
186
74.465
33.852
−29.845
1.00
20.44
B
C


ATOM
5898
CG
HIS
B
186
73.887
33.118
−28.679
1.00
20.44
B
C


ATOM
5899
ND1
HIS
B
186
73.618
31.798
−28.715
1.00
18.19
B
N


ATOM
5900
CE1
HIS
B
186
73.153
31.411
−27.516
1.00
22.14
B
C


ATOM
5901
NE2
HIS
B
186
73.154
32.481
−26.694
1.00
19.30
B
N


ATOM
5902
CD2
HIS
B
186
73.606
33.548
−27.379
1.00
23.03
B
C


ATOM
5903
C
HIS
B
186
72.304
34.932
−30.579
1.00
22.29
B
C


ATOM
5904
O
HIS
B
186
71.182
34.436
−30.457
1.00
22.93
B
O


ATOM
5905
N
ALA
B
187
72.534
36.235
−30.422
1.00
21.36
B
N


ATOM
5906
CA
ALA
B
187
71.490
37.178
−30.007
1.00
20.58
B
C


ATOM
5907
CB
ALA
B
187
72.124
38.559
−29.786
1.00
18.30
B
C


ATOM
5908
C
ALA
B
187
70.331
37.265
−31.017
1.00
22.35
B
C


ATOM
5909
O
ALA
B
187
69.153
37.323
−30.633
1.00
20.93
B
O


ATOM
5910
N
LEU
B
188
70.681
37.291
−32.302
1.00
22.80
B
N


ATOM
5911
CA
LEU
B
188
69.690
37.432
−33.372
1.00
24.02
B
C


ATOM
5912
CB
LEU
B
188
70.352
37.870
−34.690
1.00
25.03
B
C


ATOM
5913
CG
LEU
B
188
70.966
39.274
−34.681
1.00
25.80
B
C


ATOM
5914
CD1
LEU
B
188
71.784
39.523
−35.920
1.00
25.59
B
C


ATOM
5915
CD2
LEU
B
188
69.896
40.343
−34.546
1.00
26.13
B
C


ATOM
5916
C
LEU
B
188
68.918
36.139
−33.529
1.00
25.43
B
C


ATOM
5917
O
LEU
B
188
67.736
36.173
−33.805
1.00
28.67
B
O


ATOM
5918
N
GLU
B
189
69.556
35.000
−33.279
1.00
25.97
B
N


ATOM
5919
CA
GLU
B
189
68.823
33.739
−33.192
1.00
24.23
B
C


ATOM
5920
CB
GLU
B
189
69.789
32.591
−32.848
1.00
22.91
B
C


ATOM
5921
CG
GLU
B
189
69.154
31.206
−32.838
1.00
27.18
B
C


ATOM
5922
CD
GLU
B
189
70.073
30.113
−32.302
1.00
37.99
B
C


ATOM
5923
OE1
GLU
B
189
70.636
30.254
−31.179
1.00
34.64
B
O


ATOM
5924
OE2
GLU
B
189
70.220
29.090
−33.003
1.00
38.22
B
O


ATOM
5925
C
GLU
B
189
67.659
33.839
−32.160
1.00
26.47
B
C


ATOM
5926
O
GLU
B
189
66.497
33.540
−32.467
1.00
21.27
B
O


ATOM
5927
N
LEU
B
190
67.968
34.235
−30.932
1.00
22.17
B
N


ATOM
5928
CA
LEU
B
190
66.943
34.387
−29.896
1.00
20.33
B
C


ATOM
5929
CB
LEU
B
190
66.553
33.018
−29.345
1.00
17.23
B
C


ATOM
5930
CG
LEU
B
190
65.348
32.872
−28.434
1.00
18.56
B
C


ATOM
5931
CD1
LEU
B
190
64.025
33.102
−29.244
1.00
19.80
B
C


ATOM
5932
CD2
LEU
B
190
65.366
31.442
−27.832
1.00
18.21
B
C


ATOM
5933
C
LEU
B
190
67.575
35.203
−28.765
1.00
20.27
B
C


ATOM
5934
O
LEU
B
190
68.699
34.959
−28.379
1.00
17.38
B
O


ATOM
5935
N
PRO
B
191
66.858
36.149
−28.221
1.00
17.86
B
N


ATOM
5936
CA
PRO
B
191
67.456
36.948
−27.164
1.00
16.52
B
C


ATOM
5937
CB
PRO
B
191
66.496
38.137
−27.063
1.00
19.92
B
C


ATOM
5938
CG
PRO
B
191
65.224
37.601
−27.464
1.00
19.34
B
C


ATOM
5939
CD
PRO
B
191
65.550
36.666
−28.621
1.00
19.66
B
C


ATOM
5940
C
PRO
B
191
67.511
36.262
−25.844
1.00
18.58
B
C


ATOM
5941
O
PRO
B
191
66.763
35.292
−25.605
1.00
21.63
B
O


ATOM
5942
N
LEU
B
192
68.375
36.772
−24.967
1.00
19.06
B
N


ATOM
5943
CA
LEU
B
192
68.601
36.175
−23.667
1.00
19.22
B
C


ATOM
5944
CB
LEU
B
192
69.528
37.033
−22.797
1.00
24.31
B
C


ATOM
5945
CG
LEU
B
192
71.015
36.962
−23.073
1.00
33.33
B
C


ATOM
5946
CD1
LEU
B
192
71.710
38.156
−22.370
1.00
30.34
B
C


ATOM
5947
CD2
LEU
B
192
71.565
35.596
−22.603
1.00
26.99
B
C


ATOM
5948
C
LEU
B
192
67.322
35.980
−22.898
1.00
19.34
B
C


ATOM
5949
O
LEU
B
192
67.139
34.963
−22.257
1.00
16.96
B
O


ATOM
5950
N
HIS
B
193
66.459
36.983
−22.949
1.00
15.46
B
N


ATOM
5951
CA
HIS
B
193
65.235
36.966
−22.174
1.00
18.69
B
C


ATOM
5952
CB
HIS
B
193
64.544
38.336
−22.282
1.00
15.11
B
C


ATOM
5953
CG
HIS
B
193
63.405
38.528
−21.297
1.00
15.94
B
C


ATOM
5954
ND1
HIS
B
193
63.574
38.450
−19.952
1.00
18.12
B
N


ATOM
5955
CE1
HIS
B
193
62.387
38.650
−19.339
1.00
17.94
B
C


ATOM
5956
NE2
HIS
B
193
61.468
38.902
−20.292
1.00
18.67
B
N


ATOM
5957
CD2
HIS
B
193
62.071
38.835
−21.506
1.00
16.13
B
C


ATOM
5958
C
HIS
B
193
64.294
35.838
−22.558
1.00
13.46
B
C


ATOM
5959
O
HIS
B
193
63.440
35.441
−21.772
1.00
15.85
B
O


ATOM
5960
N
ARG
B
194
64.435
35.332
−23.771
1.00
13.24
B
N


ATOM
5961
CA
ARG
B
194
63.605
34.246
−24.281
1.00
14.54
B
C


ATOM
5962
CB
ARG
B
194
63.098
34.605
−25.686
1.00
15.59
B
C


ATOM
5963
CG
ARG
B
194
62.187
35.795
−25.690
1.00
15.21
B
C


ATOM
5964
CD
ARG
B
194
61.533
36.037
−27.017
1.00
17.56
B
C


ATOM
5965
NE
ARG
B
194
60.702
37.247
−26.963
1.00
19.45
B
N


ATOM
5966
CZ
ARG
B
194
60.048
37.772
−27.995
1.00
22.26
B
C


ATOM
5967
NH1
ARG
B
194
60.111
37.194
−29.191
1.00
20.50
B
N


ATOM
5968
NH2
ARG
B
194
59.276
38.847
−27.803
1.00
19.22
B
N


ATOM
5969
C
ARG
B
194
64.269
32.862
−24.328
1.00
15.56
B
C


ATOM
5970
O
ARG
B
194
63.579
31.874
−24.549
1.00
18.90
B
O


ATOM
5971
N
ARG
B
195
65.573
32.781
−24.102
1.00
17.67
B
N


ATOM
5972
CA
ARG
B
195
66.306
31.507
−24.154
1.00
17.08
B
C


ATOM
5973
CB
ARG
B
195
67.754
31.754
−24.595
1.00
19.12
B
C


ATOM
5974
CG
ARG
B
195
68.465
30.472
−25.018
1.00
20.16
B
C


ATOM
5975
CD
ARG
B
195
69.877
30.743
−25.510
1.00
18.78
B
C


ATOM
5976
NE
ARG
B
195
69.946
31.704
−26.624
1.00
21.88
B
N


ATOM
5977
CZ
ARG
B
195
69.991
31.384
−27.920
1.00
27.25
B
C


ATOM
5978
NH1
ARG
B
195
69.990
30.116
−28.303
1.00
25.88
B
N


ATOM
5979
NH2
ARG
B
195
70.068
32.335
−28.847
1.00
28.28
B
N


ATOM
5980
C
ARG
B
195
66.257
30.789
−22.798
1.00
16.57
B
C


ATOM
5981
O
ARG
B
195
66.281
31.432
−21.726
1.00
15.80
B
O


ATOM
5982
N
THR
B
196
66.158
29.470
−22.830
1.00
15.49
B
N


ATOM
5983
CA
THR
B
196
66.133
28.692
−21.605
1.00
15.97
B
C


ATOM
5984
CB
THR
B
196
65.787
27.202
−21.860
1.00
20.73
B
C


ATOM
5985
OG1
THR
B
196
66.683
26.672
−22.813
1.00
23.79
B
O


ATOM
5986
CG2
THR
B
196
64.342
27.043
−22.414
1.00
18.21
B
C


ATOM
5987
C
THR
B
196
67.509
28.817
−20.947
1.00
14.24
B
C


ATOM
5988
O
THR
B
196
68.506
29.050
−21.614
1.00
14.38
B
O


ATOM
5989
N
GLN
B
197
67.552
28.610
−19.650
1.00
15.82
B
N


ATOM
5990
CA
GLN
B
197
68.779
28.794
−18.872
1.00
15.13
B
C


ATOM
5991
CB
GLN
B
197
68.398
28.731
−17.388
1.00
16.40
B
C


ATOM
5992
CG
GLN
B
197
69.313
29.536
−16.402
1.00
23.34
B
C


ATOM
5993
CD
GLN
B
197
70.400
28.668
−15.827
1.00
19.25
B
C


ATOM
5994
OE1
GLN
B
197
70.750
27.639
−16.413
1.00
17.54
B
O


ATOM
5995
NE2
GLN
B
197
70.933
29.055
−14.672
1.00
19.64
B
N


ATOM
5996
C
GLN
B
197
69.912
27.794
−19.253
1.00
15.38
B
C


ATOM
5997
O
GLN
B
197
71.040
28.214
−19.543
1.00
15.99
B
O


ATOM
5998
N
ARG
B
198
69.631
26.487
−19.261
1.00
15.88
B
N


ATOM
5999
CA
ARG
B
198
70.650
25.503
−19.628
1.00
15.76
B
C


ATOM
6000
CB
ARG
B
198
70.151
24.068
−19.495
1.00
17.18
B
C


ATOM
6001
CG
ARG
B
198
69.747
23.686
−18.071
1.00
17.17
B
C


ATOM
6002
CD
ARG
B
198
70.987
23.505
−17.154
1.00
18.00
B
C


ATOM
6003
NE
ARG
B
198
71.390
24.755
−16.491
1.00
17.03
B
N


ATOM
6004
CZ
ARG
B
198
72.436
24.877
−15.679
1.00
18.12
B
C


ATOM
6005
NH1
ARG
B
198
73.201
23.837
−15.422
1.00
18.07
B
N


ATOM
6006
NH2
ARG
B
198
72.714
26.047
−15.111
1.00
18.32
B
N


ATOM
6007
C
ARG
B
198
71.191
25.712
−21.020
1.00
14.67
B
C


ATOM
6008
O
ARG
B
198
72.393
25.618
−21.196
1.00
16.24
B
O


ATOM
6009
N
LEU
B
199
70.345
26.007
−22.008
1.00
15.86
B
N


ATOM
6010
CA
LEU
B
199
70.846
26.282
−23.373
1.00
17.59
B
C


ATOM
6011
CB
LEU
B
199
69.741
26.411
−24.414
1.00
23.09
B
C


ATOM
6012
CG
LEU
B
199
69.172
25.097
−24.927
1.00
25.48
B
C


ATOM
6013
CD1
LEU
B
199
68.001
25.403
−25.846
1.00
29.15
B
C


ATOM
6014
CD2
LEU
B
199
70.249
24.258
−25.668
1.00
23.01
B
C


ATOM
6015
C
LEU
B
199
71.728
27.501
−23.423
1.00
14.99
B
C


ATOM
6016
O
LEU
B
199
72.779
27.465
−24.043
1.00
17.26
B
O


ATOM
6017
N
GLU
B
200
71.365
28.574
−22.717
1.00
17.19
B
N


ATOM
6018
CA
GLU
B
200
72.262
29.716
−22.634
1.00
16.80
B
C


ATOM
6019
CB
GLU
B
200
71.603
30.885
−21.903
1.00
18.56
B
C


ATOM
6020
CG
GLU
B
200
72.470
32.138
−21.838
1.00
19.52
B
C


ATOM
6021
CD
GLU
B
200
73.011
32.611
−23.208
1.00
23.00
B
C


ATOM
6022
OE1
GLU
B
200
72.365
32.402
−24.243
1.00
28.08
B
O


ATOM
6023
OE2
GLU
B
200
74.115
33.177
−23.238
1.00
26.36
B
O


ATOM
6024
C
GLU
B
200
73.598
29.332
−21.962
1.00
15.03
B
C


ATOM
6025
O
GLU
B
200
74.654
29.744
−22.419
1.00
16.93
B
O


ATOM
6026
N
ALA
B
201
73.537
28.572
−20.869
1.00
16.50
B
N


ATOM
6027
CA
ALA
B
201
74.741
28.162
−20.131
1.00
14.91
B
C


ATOM
6028
CB
ALA
B
201
74.376
27.366
−18.864
1.00
15.26
B
C


ATOM
6029
C
ALA
B
201
75.712
27.386
−21.014
1.00
14.98
B
C


ATOM
6030
O
ALA
B
201
76.902
27.711
−21.065
1.00
16.94
B
O


ATOM
6031
N
VAL
B
202
75.223
26.385
−21.734
1.00
16.65
B
N


ATOM
6032
CA
VAL
B
202
76.110
25.533
−22.524
1.00
18.24
B
C


ATOM
6033
CB
VAL
B
202
75.413
24.228
−23.033
1.00
20.43
B
C


ATOM
6034
CG1
VAL
B
202
74.512
24.514
−24.187
1.00
19.20
B
C


ATOM
6035
CG2
VAL
B
202
76.542
23.142
−23.429
1.00
18.68
B
C


ATOM
6036
C
VAL
B
202
76.767
26.327
−23.641
1.00
18.74
B
C


ATOM
6037
O
VAL
B
202
77.952
26.163
−23.923
1.00
19.25
B
O


ATOM
6038
N
TRP
B
203
76.046
27.280
−24.200
1.00
20.00
B
N


ATOM
6039
CA
TRP
B
203
76.616
28.127
−25.231
1.00
19.92
B
C


ATOM
6040
CB
TRP
B
203
75.497
28.878
−25.969
1.00
23.20
B
C


ATOM
6041
CG
TRP
B
203
76.048
29.658
−27.134
1.00
22.71
B
C


ATOM
6042
CD1
TRP
B
203
76.157
29.240
−28.460
1.00
23.74
B
C


ATOM
6043
NE1
TRP
B
203
76.707
30.213
−29.236
1.00
21.14
B
N


ATOM
6044
CE2
TRP
B
203
77.022
31.290
−28.488
1.00
22.74
B
C


ATOM
6045
CD2
TRP
B
203
76.600
31.001
−27.115
1.00
21.85
B
C


ATOM
6046
CE3
TRP
B
203
76.800
31.961
−26.132
1.00
26.65
B
C


ATOM
6047
CZ3
TRP
B
203
77.379
33.179
−26.499
1.00
22.71
B
C


ATOM
6048
CH2
TRP
B
203
77.788
33.427
−27.820
1.00
24.24
B
C


ATOM
6049
CZ2
TRP
B
203
77.604
32.487
−28.838
1.00
23.57
B
C


ATOM
6050
C
TRP
B
203
77.601
29.110
−24.635
1.00
20.87
B
C


ATOM
6051
O
TRP
B
203
78.734
29.297
−25.144
1.00
17.52
B
O


ATOM
6052
N
SER
B
204
77.199
29.771
−23.546
1.00
15.45
B
N


ATOM
6053
CA
SER
B
204
78.083
30.775
−22.940
1.00
16.11
B
C


ATOM
6054
CB
SER
B
204
77.362
31.546
−21.819
1.00
17.27
B
C


ATOM
6055
OG
SER
B
204
76.568
32.572
−22.375
1.00
19.39
B
O


ATOM
6056
C
SER
B
204
79.370
30.173
−22.395
1.00
15.39
B
C


ATOM
6057
O
SER
B
204
80.394
30.835
−22.391
1.00
16.11
B
O


ATOM
6058
N
ILE
B
205
79.319
28.938
−21.899
1.00
16.43
B
N


ATOM
6059
CA
ILE
B
205
80.503
28.343
−21.295
1.00
16.59
B
C


ATOM
6060
CB
ILE
B
205
80.192
27.060
−20.494
1.00
14.72
B
C


ATOM
6061
CG1
ILE
B
205
79.467
27.430
−19.188
1.00
15.63
B
C


ATOM
6062
CD1
ILE
B
205
78.694
26.305
−18.505
1.00
15.15
B
C


ATOM
6063
CG2
ILE
B
205
81.507
26.330
−20.134
1.00
16.76
B
C


ATOM
6064
C
ILE
B
205
81.523
28.084
−22.404
1.00
20.64
B
C


ATOM
6065
O
ILE
B
205
82.699
28.395
−22.254
1.00
15.38
B
O


ATOM
6066
N
GLU
B
206
81.056
27.531
−23.517
1.00
21.81
B
N


ATOM
6067
CA
GLU
B
206
81.889
27.373
−24.707
1.00
22.45
B
C


ATOM
6068
CB
GLU
B
206
81.087
26.726
−25.840
1.00
24.56
B
C


ATOM
6069
CG
GLU
B
206
81.899
26.453
−27.146
1.00
31.17
B
C


ATOM
6070
CD
GLU
B
206
83.163
25.580
−26.937
1.00
42.25
B
C


ATOM
6071
OE1
GLU
B
206
83.240
24.815
−25.947
1.00
39.81
B
O


ATOM
6072
OE2
GLU
B
206
84.087
25.656
−27.780
1.00
51.64
B
O


ATOM
6073
C
GLU
B
206
82.446
28.714
−25.167
1.00
20.41
B
C


ATOM
6074
O
GLU
B
206
83.636
28.830
−25.447
1.00
23.72
B
O


ATOM
6075
N
ALA
B
207
81.617
29.746
−25.249
1.00
17.21
B
N


ATOM
6076
CA
ALA
B
207
82.128
31.050
−25.707
1.00
17.73
B
C


ATOM
6077
CB
ALA
B
207
81.011
32.050
−25.881
1.00
16.31
B
C


ATOM
6078
C
ALA
B
207
83.152
31.592
−24.731
1.00
21.22
B
C


ATOM
6079
O
ALA
B
207
84.192
32.115
−25.126
1.00
21.97
B
O


ATOM
6080
N
TYR
B
208
82.868
31.451
−23.433
1.00
17.91
B
N


ATOM
6081
CA
TYR
B
208
83.709
32.070
−22.430
1.00
19.00
B
C


ATOM
6082
CB
TYR
B
208
83.017
32.016
−21.068
1.00
17.47
B
C


ATOM
6083
CG
TYR
B
208
83.563
32.956
−20.061
1.00
17.44
B
C


ATOM
6084
CD1
TYR
B
208
83.470
34.325
−20.231
1.00
22.19
B
C


ATOM
6085
CE1
TYR
B
208
84.002
35.205
−19.275
1.00
22.79
B
C


ATOM
6086
CZ
TYR
B
208
84.618
34.696
−18.160
1.00
20.52
B
C


ATOM
6087
OH
TYR
B
208
85.160
35.517
−17.199
1.00
22.16
B
O


ATOM
6088
CE2
TYR
B
208
84.712
33.343
−17.982
1.00
22.62
B
C


ATOM
6089
CD2
TYR
B
208
84.191
32.482
−18.924
1.00
20.39
B
C


ATOM
6090
C
TYR
B
208
85.121
31.455
−22.382
1.00
21.14
B
C


ATOM
6091
O
TYR
B
208
86.094
32.163
−22.176
1.00
23.19
B
O


ATOM
6092
N
ARG
B
209
85.230
30.141
−22.570
1.00
23.40
B
N


ATOM
6093
CA
ARG
B
209
86.503
29.467
−22.415
1.00
23.18
B
C


ATOM
6094
CB
ARG
B
209
86.333
27.956
−22.281
1.00
22.22
B
C


ATOM
6095
CG
ARG
B
209
85.955
27.249
−23.546
1.00
22.39
B
C


ATOM
6096
CD
ARG
B
209
85.367
25.888
−23.242
1.00
21.92
B
C


ATOM
6097
NE
ARG
B
209
86.318
25.065
−22.510
1.00
19.65
B
N


ATOM
6098
CZ
ARG
B
209
86.010
23.941
−21.872
1.00
19.54
B
C


ATOM
6099
NH1
ARG
B
209
84.767
23.478
−21.825
1.00
18.84
B
N


ATOM
6100
NH2
ARG
B
209
86.945
23.290
−21.230
1.00
19.02
B
N


ATOM
6101
C
ARG
B
209
87.437
29.794
−23.570
1.00
23.18
B
C


ATOM
6102
O
ARG
B
209
88.616
29.604
−23.442
1.00
26.17
B
O


ATOM
6103
N
LYS
B
210
86.903
30.300
−24.674
1.00
25.22
B
N


ATOM
6104
CA
LYS
B
210
87.726
30.739
−25.797
1.00
29.87
B
C


ATOM
6105
CB
LYS
B
210
86.933
30.694
−27.098
1.00
26.62
B
C


ATOM
6106
CG
LYS
B
210
86.471
29.315
−27.446
1.00
28.26
B
C


ATOM
6107
CD
LYS
B
210
86.122
29.195
−28.922
1.00
27.77
B
C


ATOM
6108
CE
LYS
B
210
84.884
28.370
−29.132
1.00
28.89
B
C


ATOM
6109
NZ
LYS
B
210
83.709
29.236
−29.267
1.00
28.19
B
N


ATOM
6110
C
LYS
B
210
88.299
32.128
−25.637
1.00
30.55
B
C


ATOM
6111
O
LYS
B
210
89.179
32.493
−26.386
1.00
34.12
B
O


ATOM
6112
N
LYS
B
211
87.806
32.915
−24.690
1.00
31.31
B
N


ATOM
6113
CA
LYS
B
211
88.390
34.236
−24.440
1.00
37.33
B
C


ATOM
6114
CB
LYS
B
211
87.432
35.096
−23.629
1.00
38.53
B
C


ATOM
6115
CG
LYS
B
211
86.127
35.407
−24.298
1.00
38.75
B
C


ATOM
6116
CD
LYS
B
211
85.194
36.134
−23.326
1.00
48.24
B
C


ATOM
6117
CE
LYS
B
211
85.650
37.563
−23.032
1.00
61.03
B
C


ATOM
6118
NZ
LYS
B
211
85.287
37.988
−21.653
1.00
72.73
B
N


ATOM
6119
C
LYS
B
211
89.701
34.097
−23.667
1.00
34.45
B
C


ATOM
6120
O
LYS
B
211
89.743
33.398
−22.650
1.00
33.64
B
O


ATOM
6121
N
GLU
B
212
90.759
34.771
−24.126
1.00
35.44
B
N


ATOM
6122
CA
GLU
B
212
92.064
34.717
−23.441
1.00
40.21
B
C


ATOM
6123
CB
GLU
B
212
93.157
35.413
−24.264
1.00
44.48
B
C


ATOM
6124
CG
GLU
B
212
94.488
35.596
−23.507
1.00
49.74
B
C


ATOM
6125
CD
GLU
B
212
95.705
35.662
−24.412
1.00
50.52
B
C


ATOM
6126
OE1
GLU
B
212
95.560
36.001
−25.603
1.00
48.70
B
O


ATOM
6127
OE2
GLU
B
212
96.814
35.368
−23.923
1.00
55.77
B
O


ATOM
6128
C
GLU
B
212
92.022
35.318
−22.029
1.00
36.75
B
C


ATOM
6129
O
GLU
B
212
92.768
34.897
−21.139
1.00
34.64
B
O


ATOM
6130
N
ASP
B
213
91.138
36.287
−21.834
1.00
33.09
B
N


ATOM
6131
CA
ASP
B
213
91.023
37.008
−20.568
1.00
30.97
B
C


ATOM
6132
CB
ASP
B
213
90.829
38.488
−20.878
1.00
33.35
B
C


ATOM
6133
CG
ASP
B
213
89.668
38.718
−21.827
1.00
43.49
B
C


ATOM
6134
OD1
ASP
B
213
89.547
37.944
−22.806
1.00
50.90
B
O


ATOM
6135
OD2
ASP
B
213
88.868
39.639
−21.587
1.00
52.59
B
O


ATOM
6136
C
ASP
B
213
89.842
36.515
−19.703
1.00
29.08
B
C


ATOM
6137
O
ASP
B
213
89.426
37.227
−18.793
1.00
29.45
B
O


ATOM
6138
N
ALA
B
214
89.304
35.324
−19.983
1.00
21.49
B
N


ATOM
6139
CA
ALA
B
214
88.231
34.735
−19.170
1.00
23.04
B
C


ATOM
6140
CB
ALA
B
214
87.829
33.366
−19.727
1.00
24.25
B
C


ATOM
6141
C
ALA
B
214
88.686
34.574
−17.737
1.00
21.97
B
C


ATOM
6142
O
ALA
B
214
89.812
34.165
−17.490
1.00
18.38
B
O


ATOM
6143
N
ASN
B
215
87.822
34.865
−16.772
1.00
21.39
B
N


ATOM
6144
CA
ASN
B
215
88.184
34.596
−15.380
1.00
20.57
B
C


ATOM
6145
CB
ASN
B
215
87.172
35.209
−14.413
1.00
21.21
B
C


ATOM
6146
CG
ASN
B
215
87.631
35.118
−12.990
1.00
23.78
B
C


ATOM
6147
OD1
ASN
B
215
87.544
34.067
−12.352
1.00
21.84
B
O


ATOM
6148
ND2
ASN
B
215
88.160
36.213
−12.483
1.00
30.68
B
N


ATOM
6149
C
ASN
B
215
88.245
33.083
−15.185
1.00
19.49
B
C


ATOM
6150
O
ASN
B
215
87.251
32.411
−15.394
1.00
17.69
B
O


ATOM
6151
N
GLN
B
216
89.388
32.554
−14.771
1.00
15.50
B
N


ATOM
6152
CA
GLN
B
216
89.567
31.099
−14.674
1.00
17.45
B
C


ATOM
6153
CB
GLN
B
216
91.057
30.710
−14.607
1.00
19.58
B
C


ATOM
6154
CG
GLN
B
216
91.873
31.067
−15.868
1.00
23.01
B
C


ATOM
6155
CD
GLN
B
216
91.223
30.547
−17.164
1.00
27.82
B
C


ATOM
6156
OE1
GLN
B
216
91.277
29.346
−17.475
1.00
28.73
B
O


ATOM
6157
NE2
GLN
B
216
90.588
31.453
−17.914
1.00
26.91
B
N


ATOM
6158
C
GLN
B
216
88.800
30.499
−13.490
1.00
16.75
B
C


ATOM
6159
O
GLN
B
216
88.333
29.345
−13.555
1.00
14.88
B
O


ATOM
6160
N
VAL
B
217
88.654
31.273
−12.416
1.00
13.21
B
N


ATOM
6161
CA
VAL
B
217
87.857
30.809
−11.286
1.00
13.06
B
C


ATOM
6162
CB
VAL
B
217
87.984
31.751
−10.040
1.00
14.93
B
C


ATOM
6163
CG1
VAL
B
217
86.939
31.390
−8.994
1.00
12.99
B
C


ATOM
6164
CG2
VAL
B
217
89.391
31.674
−9.443
1.00
12.34
B
C


ATOM
6165
C
VAL
B
217
86.403
30.651
−11.709
1.00
13.42
B
C


ATOM
6166
O
VAL
B
217
85.782
29.594
−11.470
1.00
13.08
B
O


ATOM
6167
N
LEU
B
218
85.867
31.665
−12.384
1.00
12.11
B
N


ATOM
6168
CA
LEU
B
218
84.466
31.647
−12.768
1.00
13.87
B
C


ATOM
6169
CB
LEU
B
218
84.045
33.022
−13.323
1.00
14.78
B
C


ATOM
6170
CG
LEU
B
218
82.600
33.252
−13.772
1.00
16.06
B
C


ATOM
6171
CD1
LEU
B
218
81.654
33.022
−12.598
1.00
14.64
B
C


ATOM
6172
CD2
LEU
B
218
82.436
34.651
−14.330
1.00
13.89
B
C


ATOM
6173
C
LEU
B
218
84.238
30.559
−13.810
1.00
15.09
B
C


ATOM
6174
O
LEU
B
218
83.244
29.833
−13.757
1.00
15.71
B
O


ATOM
6175
N
LEU
B
219
85.139
30.459
−14.781
1.00
14.94
B
N


ATOM
6176
CA
LEU
B
219
85.027
29.452
−15.827
1.00
15.31
B
C


ATOM
6177
CB
LEU
B
219
86.161
29.580
−16.840
1.00
16.77
B
C


ATOM
6178
CG
LEU
B
219
86.283
28.499
−17.925
1.00
17.35
B
C


ATOM
6179
CD1
LEU
B
219
84.975
28.362
−18.681
1.00
16.73
B
C


ATOM
6180
CD2
LEU
B
219
87.489
28.794
−18.869
1.00
19.01
B
C


ATOM
6181
C
LEU
B
219
85.030
28.057
−15.230
1.00
13.44
B
C


ATOM
6182
O
LEU
B
219
84.175
27.236
−15.559
1.00
16.42
B
O


ATOM
6183
N
GLU
B
220
85.986
27.779
−14.362
1.00
12.24
B
N


ATOM
6184
CA
GLU
B
220
86.071
26.453
−13.760
1.00
14.83
B
C


ATOM
6185
CB
GLU
B
220
87.308
26.297
−12.882
1.00
16.56
B
C


ATOM
6186
CG
GLU
B
220
87.391
24.892
−12.308
1.00
18.99
B
C


ATOM
6187
CD
GLU
B
220
88.677
24.606
−11.570
1.00
23.45
B
C


ATOM
6188
OE1
GLU
B
220
89.457
25.554
−11.301
1.00
23.25
B
O


ATOM
6189
OE2
GLU
B
220
88.882
23.412
−11.258
1.00
23.06
B
O


ATOM
6190
C
GLU
B
220
84.803
26.118
−12.958
1.00
15.88
B
C


ATOM
6191
O
GLU
B
220
84.271
25.018
−13.082
1.00
14.54
B
O


ATOM
6192
N
LEU
B
221
84.285
27.079
−12.190
1.00
14.47
B
N


ATOM
6193
CA
LEU
B
221
83.028
26.866
−11.436
1.00
12.29
B
C


ATOM
6194
CB
LEU
B
221
82.737
28.081
−10.553
1.00
11.47
B
C


ATOM
6195
CG
LEU
B
221
81.493
27.994
−9.676
1.00
10.80
B
C


ATOM
6196
CD1
LEU
B
221
81.598
26.866
−8.638
1.00
10.25
B
C


ATOM
6197
CD2
LEU
B
221
81.160
29.353
−8.998
1.00
11.71
B
C


ATOM
6198
C
LEU
B
221
81.842
26.603
−12.364
1.00
12.09
B
C


ATOM
6199
O
LEU
B
221
81.046
25.681
−12.123
1.00
15.58
B
O


ATOM
6200
N
ALA
B
222
81.728
27.383
−13.432
1.00
11.03
B
N


ATOM
6201
CA
ALA
B
222
80.635
27.213
−14.378
1.00
14.42
B
C


ATOM
6202
CB
ALA
B
222
80.686
28.281
−15.467
1.00
11.29
B
C


ATOM
6203
C
ALA
B
222
80.626
25.796
−14.981
1.00
15.15
B
C


ATOM
6204
O
ALA
B
222
79.575
25.199
−15.130
1.00
14.67
B
O


ATOM
6205
N
ILE
B
223
81.795
25.250
−15.289
1.00
16.15
B
N


ATOM
6206
CA
ILE
B
223
81.880
23.889
−15.831
1.00
15.33
B
C


ATOM
6207
CB
ILE
B
223
83.302
23.575
−16.340
1.00
15.08
B
C


ATOM
6208
CG1
ILE
B
223
83.590
24.391
−17.596
1.00
13.36
B
C


ATOM
6209
CD1
ILE
B
223
85.065
24.343
−18.024
1.00
15.70
B
C


ATOM
6210
CG2
ILE
B
223
83.453
22.071
−16.630
1.00
13.45
B
C


ATOM
6211
C
ILE
B
223
81.491
22.862
−14.774
1.00
14.47
B
C


ATOM
6212
O
ILE
B
223
80.665
21.992
−15.007
1.00
14.34
B
O


ATOM
6213
N
LEU
B
224
82.062
23.009
−13.587
1.00
15.34
B
N


ATOM
6214
CA
ALEU
B
224
81.784
22.109
−12.486
0.50
16.13
B
C


ATOM
6215
CA
BLEU
B
224
81.779
22.060
−12.526
0.50
16.37
B
C


ATOM
6216
CB
ALEU
B
224
82.552
22.593
−11.262
0.50
16.37
B
C


ATOM
6217
CB
BLEU
B
224
82.640
22.335
−11.290
0.50
16.95
B
C


ATOM
6218
CG
ALEU
B
224
82.640
21.657
−10.072
0.50
16.80
B
C


ATOM
6219
CG
BLEU
B
224
84.152
22.112
−11.454
0.50
17.31
B
C


ATOM
6220
CD1
ALEU
B
224
83.895
22.006
−9.297
0.50
15.21
B
C


ATOM
6221
CD1
BLEU
B
224
84.830
22.348
−10.119
0.50
16.93
B
C


ATOM
6222
CD2
ALEU
B
224
81.395
21.740
−9.189
0.50
14.02
B
C


ATOM
6223
CD2
BLEU
B
224
84.502
20.713
−12.008
0.50
17.10
B
C


ATOM
6224
C
LEU
B
224
80.276
22.060
−12.196
1.00
17.10
B
C


ATOM
6225
O
LEU
B
224
79.666
20.988
−12.081
1.00
14.62
B
O


ATOM
6226
N
ASP
B
225
79.678
23.237
−12.062
1.00
14.86
B
N


ATOM
6227
CA
ASP
B
225
78.271
23.337
−11.693
1.00
14.10
B
C


ATOM
6228
CB
ASP
B
225
77.864
24.805
−11.425
1.00
14.24
B
C


ATOM
6229
CG
ASP
B
225
76.624
24.906
−10.544
1.00
15.89
B
C


ATOM
6230
OD1
ASP
B
225
76.787
24.757
−9.307
1.00
16.14
B
O


ATOM
6231
OD2
ASP
B
225
75.494
25.104
−11.079
1.00
16.81
B
O


ATOM
6232
C
ASP
B
225
77.365
22.750
−12.777
1.00
13.39
B
C


ATOM
6233
O
ASP
B
225
76.487
21.982
−12.464
1.00
15.58
B
O


ATOM
6234
N
TYR
B
226
77.603
23.096
−14.044
1.00
11.94
B
N


ATOM
6235
CA
TYR
B
226
76.825
22.573
−15.154
1.00
15.44
B
C


ATOM
6236
CB
TYR
B
226
77.342
23.140
−16.461
1.00
14.71
B
C


ATOM
6237
CG
TYR
B
226
76.434
22.785
−17.603
1.00
15.95
B
C


ATOM
6238
CD1
TYR
B
226
76.558
21.559
−18.265
1.00
16.89
B
C


ATOM
6239
CE1
TYR
B
226
75.730
21.218
−19.309
1.00
19.42
B
C


ATOM
6240
CZ
TYR
B
226
74.746
22.102
−19.716
1.00
21.14
B
C


ATOM
6241
OH
TYR
B
226
73.902
21.754
−20.731
1.00
22.75
B
O


ATOM
6242
CE2
TYR
B
226
74.592
23.324
−19.066
1.00
21.14
B
C


ATOM
6243
CD2
TYR
B
226
75.450
23.658
−18.023
1.00
18.76
B
C


ATOM
6244
C
TYR
B
226
76.814
21.032
−15.199
1.00
18.55
B
C


ATOM
6245
O
TYR
B
226
75.751
20.403
−15.340
1.00
14.96
B
O


ATOM
6246
N
ASN
B
227
77.993
20.438
−15.054
1.00
17.61
B
N


ATOM
6247
CA
ASN
B
227
78.132
18.972
−15.059
1.00
18.83
B
C


ATOM
6248
CB
ASN
B
227
79.592
18.545
−15.274
1.00
19.64
B
C


ATOM
6249
CG
ASN
B
227
80.065
18.832
−16.685
1.00
18.15
B
C


ATOM
6250
OD1
ASN
B
227
79.248
19.019
−17.608
1.00
20.61
B
O


ATOM
6251
ND2
ASN
B
227
81.370
18.933
−16.861
1.00
16.37
B
N


ATOM
6252
C
ASN
B
227
77.496
18.337
−13.828
1.00
20.83
B
C


ATOM
6253
O
ASN
B
227
76.846
17.296
−13.957
1.00
19.81
B
O


ATOM
6254
N
MET
B
228
77.571
19.001
−12.673
1.00
17.33
B
N


ATOM
6255
CA
AMET
B
228
76.866
18.537
−11.479
0.50
16.75
B
C


ATOM
6256
CA
BMET
B
228
76.865
18.495
−11.491
0.50
22.51
B
C


ATOM
6257
CB
AMET
B
228
77.225
19.420
−10.287
0.50
14.37
B
C


ATOM
6258
CB
BMET
B
228
77.300
19.200
−10.188
0.50
29.14
B
C


ATOM
6259
CG
AMET
B
228
76.537
19.072
−8.975
0.50
12.77
B
C


ATOM
6260
CG
BMET
B
228
76.514
20.447
−9.749
0.50
44.80
B
C


ATOM
6261
SD
AMET
B
228
76.760
20.399
−7.762
0.50
12.66
B
S


ATOM
6262
SD
BMET
B
228
75.395
20.226
−8.333
0.50
54.35
B
S


ATOM
6263
CE
AMET
B
228
75.682
21.683
−8.474
0.50
15.49
B
C


ATOM
6264
CE
BMET
B
228
74.091
19.223
−9.003
0.50
42.65
B
C


ATOM
6265
C
MET
B
228
75.342
18.530
−11.708
1.00
20.48
B
C


ATOM
6266
O
MET
B
228
74.653
17.559
−11.394
1.00
18.56
B
O


ATOM
6267
N
ILE
B
229
74.799
19.611
−12.268
1.00
17.89
B
N


ATOM
6268
CA
ILE
B
229
73.350
19.661
−12.469
1.00
15.86
B
C


ATOM
6269
CB
ILE
B
229
72.858
21.076
−12.870
1.00
16.15
B
C


ATOM
6270
CG1
ILE
B
229
73.043
22.035
−11.687
1.00
15.99
B
C


ATOM
6271
CD1
ILE
B
229
72.699
23.482
−11.988
1.00
18.39
B
C


ATOM
6272
CG2
ILE
B
229
71.358
21.045
−13.274
1.00
15.61
B
C


ATOM
6273
C
ILE
B
229
72.943
18.596
−13.495
1.00
17.35
B
C


ATOM
6274
O
ILE
B
229
71.971
17.909
−13.306
1.00
23.75
B
O


ATOM
6275
N
GLN
B
230
73.701
18.459
−14.570
1.00
17.03
B
N


ATOM
6276
CA
GLN
B
230
73.427
17.433
−15.545
1.00
22.65
B
C


ATOM
6277
CB
GLN
B
230
74.469
17.433
−16.630
1.00
23.17
B
C


ATOM
6278
CG
GLN
B
230
74.036
16.671
−17.840
1.00
24.09
B
C


ATOM
6279
CD
GLN
B
230
75.124
16.660
−18.876
1.00
25.69
B
C


ATOM
6280
OE1
GLN
B
230
76.207
16.159
−18.617
1.00
24.23
B
O


ATOM
6281
NE2
GLN
B
230
74.854
17.238
−20.050
1.00
23.11
B
N


ATOM
6282
C
GLN
B
230
73.368
16.037
−14.929
1.00
23.04
B
C


ATOM
6283
O
GLN
B
230
72.565
15.234
−15.356
1.00
23.54
B
O


ATOM
6284
N
SER
B
231
74.200
15.745
−13.941
1.00
21.41
B
N


ATOM
6285
CA
SER
B
231
74.193
14.412
−13.319
1.00
23.65
B
C


ATOM
6286
CB
SER
B
231
75.471
14.168
−12.468
1.00
23.19
B
C


ATOM
6287
OG
SER
B
231
75.384
14.886
−11.237
1.00
32.62
B
O


ATOM
6288
C
SER
B
231
72.896
14.223
−12.512
1.00
24.26
B
C


ATOM
6289
O
SER
B
231
72.344
13.112
−12.459
1.00
20.75
B
O


ATOM
6290
N
VAL
B
232
72.381
15.302
−11.929
1.00
21.48
B
N


ATOM
6291
CA
VAL
B
232
71.037
15.279
−11.328
1.00
21.24
B
C


ATOM
6292
CB
VAL
B
232
70.692
16.603
−10.628
1.00
23.83
B
C


ATOM
6293
CG1
VAL
B
232
69.270
16.592
−10.059
1.00
24.12
B
C


ATOM
6294
CG2
VAL
B
232
71.681
16.880
−9.532
1.00
20.84
B
C


ATOM
6295
C
VAL
B
232
69.986
14.952
−12.413
1.00
24.74
B
C


ATOM
6296
O
VAL
B
232
69.136
14.097
−12.209
1.00
24.73
B
O


ATOM
6297
N
TYR
B
233
70.071
15.579
−13.583
1.00
23.53
B
N


ATOM
6298
CA
TYR
B
233
69.100
15.288
−14.644
1.00
23.90
B
C


ATOM
6299
CB
TYR
B
233
69.310
16.184
−15.861
1.00
19.78
B
C


ATOM
6300
CG
TYR
B
233
69.165
17.700
−15.638
1.00
15.82
B
C


ATOM
6301
CD1
TYR
B
233
68.412
18.220
−14.594
1.00
14.87
B
C


ATOM
6302
CE1
TYR
B
233
68.281
19.609
−14.438
1.00
16.04
B
C


ATOM
6303
CZ
TYR
B
233
68.900
20.445
−15.347
1.00
15.46
B
C


ATOM
6304
OH
TYR
B
233
68.778
21.816
−15.227
1.00
19.31
B
O


ATOM
6305
CE2
TYR
B
233
69.633
19.934
−16.393
1.00
16.43
B
C


ATOM
6306
CD2
TYR
B
233
69.760
18.597
−16.540
1.00
15.50
B
C


ATOM
6307
C
TYR
B
233
69.201
13.806
−15.097
1.00
29.65
B
C


ATOM
6308
O
TYR
B
233
68.182
13.176
−15.436
1.00
24.75
B
O


ATOM
6309
N
GLN
B
234
70.415
13.260
−15.122
1.00
23.59
B
N


ATOM
6310
CA
GLN
B
234
70.614
11.879
−15.605
1.00
27.67
B
C


ATOM
6311
CB
GLN
B
234
72.083
11.615
−15.914
1.00
26.53
B
C


ATOM
6312
CG
GLN
B
234
72.547
12.384
−17.157
1.00
26.79
B
C


ATOM
6313
CD
GLN
B
234
74.045
12.416
−17.285
1.00
29.18
B
C


ATOM
6314
OE1
GLN
B
234
74.758
12.085
−16.338
1.00
32.91
B
O


ATOM
6315
NE2
GLN
B
234
74.541
12.814
−18.458
1.00
29.56
B
N


ATOM
6316
C
GLN
B
234
70.021
10.888
−14.609
1.00
23.60
B
C


ATOM
6317
O
GLN
B
234
69.391
9.907
−15.012
1.00
30.92
B
O


ATOM
6318
N
ARG
B
235
70.150
11.174
−13.319
1.00
25.13
B
N


ATOM
6319
CA
ARG
B
235
69.448
10.394
−12.303
1.00
29.36
B
C


ATOM
6320
CB
ARG
B
235
69.922
10.758
−10.898
1.00
30.29
B
C


ATOM
6321
CG
ARG
B
235
69.373
9.840
−9.811
1.00
39.19
B
C


ATOM
6322
CD
ARG
B
235
70.021
10.065
−8.460
1.00
56.79
B
C


ATOM
6323
NE
ARG
B
235
69.818
11.434
−7.971
1.00
77.67
B
N


ATOM
6324
CZ
ARG
B
235
70.759
12.383
−7.879
1.00
76.44
B
C


ATOM
6325
NH1
ARG
B
235
72.024
12.147
−8.232
1.00
76.63
B
N


ATOM
6326
NH2
ARG
B
235
70.430
13.592
−7.421
1.00
56.91
B
N


ATOM
6327
C
ARG
B
235
67.908
10.518
−12.423
1.00
30.66
B
C


ATOM
6328
O
ARG
B
235
67.200
9.523
−12.395
1.00
26.67
B
O


ATOM
6329
N
ASP
B
236
67.391
11.726
−12.567
1.00
29.85
B
N


ATOM
6330
CA
ASP
B
236
65.944
11.945
−12.764
1.00
25.23
B
C


ATOM
6331
CB
ASP
B
236
65.644
13.409
−13.134
1.00
30.33
B
C


ATOM
6332
CG
ASP
B
236
65.904
14.414
−12.004
1.00
36.62
B
C


ATOM
6333
OD1
ASP
B
236
65.910
14.039
−10.810
1.00
31.93
B
O


ATOM
6334
OD2
ASP
B
236
66.050
15.627
−12.336
1.00
28.75
B
O


ATOM
6335
C
ASP
B
236
65.437
11.096
−13.924
1.00
24.79
B
C


ATOM
6336
O
ASP
B
236
64.449
10.412
−13.797
1.00
27.76
B
O


ATOM
6337
N
LEU
B
237
66.126
11.191
−15.058
1.00
21.17
B
N


ATOM
6338
CA
LEU
B
237
65.758
10.541
−16.298
1.00
24.76
B
C


ATOM
6339
CB
LEU
B
237
66.664
11.028
−17.431
1.00
24.56
B
C


ATOM
6340
CG
LEU
B
237
66.562
10.373
−18.805
1.00
29.01
B
C


ATOM
6341
CD1
LEU
B
237
65.215
10.683
−19.447
1.00
30.62
B
C


ATOM
6342
CD2
LEU
B
237
67.720
10.818
−19.706
1.00
31.68
B
C


ATOM
6343
C
LEU
B
237
65.849
9.017
−16.213
1.00
31.40
B
C


ATOM
6344
O
LEU
B
237
65.066
8.326
−16.851
1.00
24.72
B
O


ATOM
6345
N
ARG
B
238
66.832
8.503
−15.484
1.00
28.78
B
N


ATOM
6346
CA
AARG
B
238
66.936
7.058
−15.276
0.50
33.73
B
C


ATOM
6347
CA
BARG
B
238
66.945
7.057
−15.247
0.50
35.56
B
C


ATOM
6348
CB
AARG
B
238
68.255
6.681
−14.581
0.50
31.17
B
C


ATOM
6349
CB
BARG
B
238
68.215
6.706
−14.454
0.50
35.57
B
C


ATOM
6350
CG
AARG
B
238
69.474
6.716
−15.506
0.50
30.21
B
C


ATOM
6351
CG
BARG
B
238
69.457
6.476
−15.299
0.50
37.44
B
C


ATOM
6352
CD
AARG
B
238
70.728
6.119
−14.842
0.50
33.56
B
C


ATOM
6353
CD
BARG
B
238
70.558
5.769
−14.481
0.50
41.07
B
C


ATOM
6354
NE
AARG
B
238
71.117
6.845
−13.633
0.50
32.43
B
N


ATOM
6355
NE
BARG
B
238
71.197
6.676
−13.525
0.50
42.64
B
N


ATOM
6356
CZ
AARG
B
238
72.064
7.776
−13.591
0.50
25.66
B
C


ATOM
6357
CZ
BARG
B
238
70.981
6.679
−12.210
0.50
36.62
B
C


ATOM
6358
NH1
AARG
B
238
72.731
8.098
−14.692
0.50
21.79
B
N


ATOM
6359
NH1
BARG
B
238
70.145
5.812
−11.649
0.50
30.78
B
N


ATOM
6360
NH2
AARG
B
238
72.339
8.381
−12.446
0.50
23.30
B
N


ATOM
6361
NH2
BARG
B
238
71.616
7.557
−11.450
0.50
35.02
B
N


ATOM
6362
C
ARG
B
238
65.723
6.562
−14.485
1.00
36.07
B
C


ATOM
6363
O
ARG
B
238
65.155
5.526
−14.817
1.00
32.64
B
O


ATOM
6364
N
GLU
B
239
65.307
7.314
−13.468
1.00
33.02
B
N


ATOM
6365
CA
GLU
B
239
64.140
6.922
−12.679
1.00
39.52
B
C


ATOM
6366
CB
GLU
B
239
64.051
7.764
−11.416
1.00
50.36
B
C


ATOM
6367
CG
GLU
B
239
62.899
7.375
−10.495
1.00
67.79
B
C


ATOM
6368
CD
GLU
B
239
62.986
8.033
−9.123
1.00
87.78
B
C


ATOM
6369
OE1
GLU
B
239
64.032
8.639
−8.798
1.00
81.07
B
O


ATOM
6370
OE2
GLU
B
239
61.998
7.943
−8.363
1.00
105.74
B
O


ATOM
6371
C
GLU
B
239
62.825
7.017
−13.483
1.00
44.14
B
C


ATOM
6372
O
GLU
B
239
61.941
6.160
−13.360
1.00
37.48
B
O


ATOM
6373
N
THR
B
240
62.709
8.058
−14.299
1.00
35.32
B
N


ATOM
6374
CA
THR
B
240
61.537
8.246
−15.162
1.00
32.81
B
C


ATOM
6375
CB
THR
B
240
61.473
9.688
−15.647
1.00
33.62
B
C


ATOM
6376
OG1
THR
B
240
61.233
10.512
−14.498
1.00
32.31
B
O


ATOM
6377
CG2
THR
B
240
60.347
9.909
−16.683
1.00
28.21
B
C


ATOM
6378
C
THR
B
240
61.555
7.265
−16.334
1.00
32.29
B
C


ATOM
6379
O
THR
B
240
60.508
6.883
−16.842
1.00
30.87
B
O


ATOM
6380
N
SER
B
241
62.742
6.866
−16.775
1.00
35.89
B
N


ATOM
6381
CA
SER
B
241
62.876
5.813
−17.801
1.00
36.95
B
C


ATOM
6382
CB
SER
B
241
64.332
5.642
−18.239
1.00
41.95
B
C


ATOM
6383
OG
SER
B
241
64.659
6.570
−19.256
1.00
43.35
B
O


ATOM
6384
C
SER
B
241
62.369
4.479
−17.284
1.00
30.92
B
C


ATOM
6385
O
SER
B
241
61.673
3.751
−17.983
1.00
31.23
B
O


ATOM
6386
N
ARG
B
242
62.720
4.158
−16.056
1.00
31.06
B
N


ATOM
6387
CA
ARG
B
242
62.264
2.918
−15.487
1.00
43.04
B
C


ATOM
6388
CB
ARG
B
242
62.894
2.633
−14.122
1.00
49.17
B
C


ATOM
6389
CG
ARG
B
242
64.246
1.896
−14.196
1.00
63.90
B
C


ATOM
6390
CD
ARG
B
242
64.647
1.303
−12.835
1.00
65.15
B
C


ATOM
6391
NE
ARG
B
242
64.177
2.139
−11.721
1.00
71.29
B
N


ATOM
6392
CZ
ARG
B
242
64.864
3.126
−11.141
1.00
77.59
B
C


ATOM
6393
NH1
ARG
B
242
66.099
3.431
−11.532
1.00
83.44
B
N


ATOM
6394
NH2
ARG
B
242
64.309
3.814
−10.147
1.00
76.69
B
N


ATOM
6395
C
ARG
B
242
60.762
2.955
−15.381
1.00
31.28
B
C


ATOM
6396
O
ARG
B
242
60.123
1.965
−15.642
1.00
36.75
B
O


ATOM
6397
N
TRP
B
243
60.202
4.081
−14.965
1.00
30.04
B
N


ATOM
6398
CA
TRP
B
243
58.751
4.277
−14.948
1.00
24.61
B
C


ATOM
6399
CB
TRP
B
243
58.397
5.692
−14.474
1.00
29.93
B
C


ATOM
6400
CG
TRP
B
243
56.955
6.076
−14.721
1.00
20.51
B
C


ATOM
6401
CD1
TRP
B
243
55.854
5.661
−13.998
1.00
24.00
B
C


ATOM
6402
NE1
TRP
B
243
54.712
6.162
−14.525
1.00
27.60
B
N


ATOM
6403
CE2
TRP
B
243
54.967
6.875
−15.624
1.00
19.22
B
C


ATOM
6404
CD2
TRP
B
243
56.412
6.868
−15.811
1.00
19.39
B
C


ATOM
6405
CE3
TRP
B
243
56.949
7.527
−16.889
1.00
19.69
B
C


ATOM
6406
CZ3
TRP
B
243
56.099
8.225
−17.740
1.00
22.15
B
C


ATOM
6407
CH2
TRP
B
243
54.712
8.236
−17.526
1.00
25.82
B
C


ATOM
6408
CZ2
TRP
B
243
54.132
7.543
−16.456
1.00
20.93
B
C


ATOM
6409
C
TRP
B
243
58.132
4.039
−16.310
1.00
24.26
B
C


ATOM
6410
O
TRP
B
243
57.138
3.322
−16.441
1.00
24.17
B
O


ATOM
6411
N
TRP
B
244
58.691
4.659
−17.342
1.00
20.84
B
N


ATOM
6412
CA
TRP
B
244
58.099
4.583
−18.675
1.00
19.39
B
C


ATOM
6413
CB
TRP
B
244
58.793
5.572
−19.606
1.00
22.08
B
C


ATOM
6414
CG
TRP
B
244
58.136
5.798
−20.943
1.00
24.58
B
C


ATOM
6415
CD1
TRP
B
244
58.744
5.714
−22.189
1.00
33.52
B
C


ATOM
6416
NE1
TRP
B
244
57.842
5.994
−23.184
1.00
32.99
B
N


ATOM
6417
CE2
TRP
B
244
56.626
6.277
−22.674
1.00
25.24
B
C


ATOM
6418
CD2
TRP
B
244
56.729
6.170
−21.233
1.00
24.61
B
C


ATOM
6419
CE3
TRP
B
244
55.597
6.387
−20.467
1.00
22.17
B
C


ATOM
6420
CZ3
TRP
B
244
54.404
6.694
−21.102
1.00
28.89
B
C


ATOM
6421
CH2
TRP
B
244
54.327
6.798
−22.486
1.00
30.43
B
C


ATOM
6422
CZ2
TRP
B
244
55.438
6.596
−23.299
1.00
29.37
B
C


ATOM
6423
C
TRP
B
244
58.160
3.178
−19.209
1.00
19.52
B
C


ATOM
6424
O
TRP
B
244
57.231
2.709
−19.844
1.00
15.68
B
O


ATOM
6425
N
ARG
B
245
59.256
2.487
−18.948
1.00
21.43
B
N


ATOM
6426
CA
ARG
B
245
59.400
1.083
−19.391
1.00
26.75
B
C


ATOM
6427
CB
ARG
B
245
60.834
0.594
−19.143
1.00
29.07
B
C


ATOM
6428
CG
ARG
B
245
61.766
0.748
−20.349
1.00
46.10
B
C


ATOM
6429
CD
ARG
B
245
63.055
−0.075
−20.152
1.00
52.27
B
C


ATOM
6430
NE
ARG
B
245
63.735
0.329
−18.919
1.00
64.17
B
N


ATOM
6431
CZ
ARG
B
245
64.460
1.444
−18.777
1.00
60.45
B
C


ATOM
6432
NH1
ARG
B
245
64.629
2.282
−19.802
1.00
61.89
B
N


ATOM
6433
NH2
ARG
B
245
65.017
1.732
−17.602
1.00
47.28
B
N


ATOM
6434
C
ARG
B
245
58.397
0.139
−18.696
1.00
28.71
B
C


ATOM
6435
O
ARG
B
245
57.820
−0.740
−19.320
1.00
25.38
B
O


ATOM
6436
N
ARG
B
246
58.216
0.320
−17.396
1.00
32.47
B
N


ATOM
6437
CA
ARG
B
246
57.112
−0.300
−16.645
1.00
37.98
B
C


ATOM
6438
CB
ARG
B
246
57.043
0.306
−15.234
1.00
56.60
B
C


ATOM
6439
CG
ARG
B
246
56.231
−0.484
−14.220
1.00
67.23
B
C


ATOM
6440
CD
ARG
B
246
57.128
−1.273
−13.267
1.00
74.38
B
C


ATOM
6441
NE
ARG
B
246
56.697
−2.665
−13.121
1.00
94.33
B
N


ATOM
6442
CZ
ARG
B
246
57.313
−3.572
−12.368
1.00
96.83
B
C


ATOM
6443
NH1
ARG
B
246
58.394
−3.246
−11.664
1.00
103.34
B
N


ATOM
6444
NH2
ARG
B
246
56.844
−4.810
−12.311
1.00
93.77
B
N


ATOM
6445
C
ARG
B
246
55.753
−0.116
−17.318
1.00
26.40
B
C


ATOM
6446
O
ARG
B
246
55.060
−1.073
−17.549
1.00
29.09
B
O


ATOM
6447
N
VAL
B
247
55.385
1.123
−17.622
1.00
20.94
B
N


ATOM
6448
CA
VAL
B
247
54.100
1.447
−18.239
1.00
19.96
B
C


ATOM
6449
CB
VAL
B
247
53.895
2.953
−18.359
1.00
22.94
B
C


ATOM
6450
CG1
VAL
B
247
52.618
3.271
−19.077
1.00
23.70
B
C


ATOM
6451
CG2
VAL
B
247
53.861
3.621
−16.969
1.00
28.91
B
C


ATOM
6452
C
VAL
B
247
53.969
0.743
−19.597
1.00
22.21
B
C


ATOM
6453
O
VAL
B
247
52.930
0.127
−19.893
1.00
19.85
B
O


ATOM
6454
N
GLY
B
248
55.038
0.804
−20.399
1.00
26.35
B
N


ATOM
6455
CA
GLY
B
248
55.212
−0.050
−21.602
1.00
25.85
B
C


ATOM
6456
C
GLY
B
248
54.266
0.239
−22.739
1.00
22.97
B
C


ATOM
6457
O
GLY
B
248
53.999
−0.599
−23.598
1.00
27.66
B
O


ATOM
6458
N
LEU
B
249
53.749
1.430
−22.753
1.00
24.10
B
N


ATOM
6459
CA
LEU
B
249
52.654
1.793
−23.645
1.00
30.34
B
C


ATOM
6460
CB
LEU
B
249
52.002
3.042
−23.086
1.00
31.09
B
C


ATOM
6461
CG
LEU
B
249
50.500
3.297
−23.109
1.00
36.14
B
C


ATOM
6462
CD1
LEU
B
249
49.670
2.120
−22.704
1.00
34.85
B
C


ATOM
6463
CD2
LEU
B
249
50.227
4.513
−22.209
1.00
35.23
B
C


ATOM
6464
C
LEU
B
249
53.133
1.991
−25.101
1.00
46.24
B
C


ATOM
6465
O
LEU
B
249
52.454
1.568
−26.054
1.00
38.44
B
O


ATOM
6466
N
ALA
B
250
54.305
2.602
−25.276
1.00
52.20
B
N


ATOM
6467
CA
ALA
B
250
54.887
2.779
−26.614
1.00
63.15
B
C


ATOM
6468
CB
ALA
B
250
56.153
3.635
−26.545
1.00
60.43
B
C


ATOM
6469
C
ALA
B
250
55.179
1.429
−27.298
1.00
64.10
B
C


ATOM
6470
O
ALA
B
250
55.044
1.311
−28.512
1.00
64.56
B
O


ATOM
6471
N
THR
B
251
55.566
0.427
−26.507
1.00
50.70
B
N


ATOM
6472
CA
THR
B
251
55.744
−0.947
−26.971
1.00
41.78
B
C


ATOM
6473
CB
THR
B
251
56.377
−1.808
−25.860
1.00
42.75
B
C


ATOM
6474
OG1
THR
B
251
57.705
−1.349
−25.620
1.00
59.83
B
O


ATOM
6475
CG2
THR
B
251
56.414
−3.278
−26.243
1.00
44.05
B
C


ATOM
6476
C
THR
B
251
54.458
−1.679
−27.377
1.00
42.26
B
C


ATOM
6477
O
THR
B
251
54.463
−2.481
−28.304
1.00
53.14
B
O


ATOM
6478
N
LYS
B
252
53.377
−1.430
−26.648
1.00
33.76
B
N


ATOM
6479
CA
LYS
B
252
52.133
−2.199
−26.779
1.00
35.99
B
C


ATOM
6480
CB
LYS
B
252
51.520
−2.436
−25.385
1.00
33.65
B
C


ATOM
6481
CG
LYS
B
252
52.400
−3.297
−24.446
1.00
32.18
B
C


ATOM
6482
CD
LYS
B
252
52.228
−4.791
−24.691
1.00
34.71
B
C


ATOM
6483
CE
LYS
B
252
53.099
−5.652
−23.754
1.00
36.30
B
C


ATOM
6484
NZ
LYS
B
252
52.740
−7.131
−23.821
1.00
35.73
B
N


ATOM
6485
C
LYS
B
252
51.117
−1.533
−27.710
1.00
31.67
B
C


ATOM
6486
O
LYS
B
252
50.178
−2.167
−28.160
1.00
43.55
B
O


ATOM
6487
N
LEU
B
253
51.315
−0.252
−27.983
1.00
27.89
B
N


ATOM
6488
CA
LEU
B
253
50.466
0.507
−28.899
1.00
31.51
B
C


ATOM
6489
CB
LEU
B
253
50.054
1.851
−28.287
1.00
29.45
B
C


ATOM
6490
CG
LEU
B
253
48.676
2.020
−27.621
1.00
36.86
B
C


ATOM
6491
CD1
LEU
B
253
48.212
0.812
−26.830
1.00
38.75
B
C


ATOM
6492
CD2
LEU
B
253
48.707
3.285
−26.767
1.00
33.51
B
C


ATOM
6493
C
LEU
B
253
51.313
0.720
−30.160
1.00
35.74
B
C


ATOM
6494
O
LEU
B
253
52.301
1.454
−30.135
1.00
37.08
B
O


ATOM
6495
N
HIS
B
254
50.903
0.076
−31.249
1.00
33.84
B
N


ATOM
6496
CA
AHIS
B
254
51.728
−0.000
−32.446
0.50
33.25
B
C


ATOM
6497
CA
BHIS
B
254
51.678
−0.001
−32.501
0.50
34.84
B
C


ATOM
6498
CB
AHIS
B
254
51.245
−1.162
−33.334
0.50
34.73
B
C


ATOM
6499
CB
BHIS
B
254
51.067
−1.032
−33.482
0.50
37.53
B
C


ATOM
6500
CG
AHIS
B
254
51.052
−2.474
−32.569
0.50
36.94
B
C


ATOM
6501
CG
BHIS
B
254
49.813
−0.555
−34.199
0.50
43.00
B
C


ATOM
6502
ND1
AHIS
B
254
50.299
−3.497
−33.039
0.50
37.35
B
N


ATOM
6503
ND1
BHIS
B
254
48.588
−1.038
−33.918
0.50
48.80
B
N


ATOM
6504
CE1
AHIS
B
254
50.307
−4.506
−32.148
0.50
33.86
B
C


ATOM
6505
CE1
BHIS
B
254
47.677
−0.434
−34.704
0.50
47.74
B
C


ATOM
6506
NE2
AHIS
B
254
51.054
−4.127
−31.097
0.50
38.53
B
N


ATOM
6507
NE2
BHIS
B
254
48.321
0.439
−35.497
0.50
39.38
B
N


ATOM
6508
CD2
AHIS
B
254
51.528
−2.881
−31.323
0.50
33.37
B
C


ATOM
6509
CD2
BHIS
B
254
49.638
0.385
−35.217
0.50
46.45
B
C


ATOM
6510
C
HIS
B
254
51.807
1.330
−33.183
1.00
33.53
B
C


ATOM
6511
O
HIS
B
254
52.796
1.603
−33.858
1.00
25.82
B
O


ATOM
6512
N
PHE
B
255
50.793
2.184
−33.031
1.00
27.23
B
N


ATOM
6513
CA
PHE
B
255
50.822
3.525
−33.632
1.00
29.21
B
C


ATOM
6514
CB
PHE
B
255
49.380
4.066
−33.854
1.00
29.73
B
C


ATOM
6515
CG
PHE
B
255
48.607
4.271
−32.575
1.00
27.91
B
C


ATOM
6516
CD1
PHE
B
255
48.736
5.449
−31.865
1.00
29.74
B
C


ATOM
6517
CE1
PHE
B
255
48.058
5.637
−30.661
1.00
35.80
B
C


ATOM
6518
CZ
PHE
B
255
47.262
4.611
−30.142
1.00
36.41
B
C


ATOM
6519
CE2
PHE
B
255
47.144
3.411
−30.838
1.00
37.91
B
C


ATOM
6520
CD2
PHE
B
255
47.817
3.245
−32.046
1.00
31.88
B
C


ATOM
6521
C
PHE
B
255
51.653
4.574
−32.841
1.00
35.02
B
C


ATOM
6522
O
PHE
B
255
51.930
5.653
−33.380
1.00
32.16
B
O


ATOM
6523
N
ALA
B
256
52.024
4.287
−31.583
1.00
36.94
B
N


ATOM
6524
CA
ALA
B
256
52.473
5.361
−30.660
1.00
44.62
B
C


ATOM
6525
CB
ALA
B
256
52.189
5.002
−29.178
1.00
38.64
B
C


ATOM
6526
C
ALA
B
256
53.939
5.730
−30.831
1.00
41.50
B
C


ATOM
6527
O
ALA
B
256
54.791
4.866
−31.042
1.00
32.37
B
O


ATOM
6528
N
ARG
B
257
54.206
7.030
−30.748
1.00
42.55
B
N


ATOM
6529
CA
ARG
B
257
55.560
7.566
−30.699
1.00
55.66
B
C


ATOM
6530
CB
ARG
B
257
55.563
9.058
−31.074
1.00
61.07
B
C


ATOM
6531
CG
ARG
B
257
55.225
9.398
−32.526
1.00
63.21
B
C


ATOM
6532
CD
ARG
B
257
55.159
10.932
−32.719
1.00
66.34
B
C


ATOM
6533
NE
ARG
B
257
53.937
11.526
−32.146
1.00
55.10
B
N


ATOM
6534
CZ
ARG
B
257
53.814
12.782
−31.707
1.00
68.91
B
C


ATOM
6535
NH1
ARG
B
257
54.841
13.630
−31.738
1.00
80.51
B
N


ATOM
6536
NH2
ARG
B
257
52.652
13.200
−31.206
1.00
69.46
B
N


ATOM
6537
C
ARG
B
257
56.107
7.437
−29.275
1.00
58.53
B
C


ATOM
6538
O
ARG
B
257
55.422
7.816
−28.316
1.00
50.74
B
O


ATOM
6539
N
ASP
B
258
57.329
6.911
−29.145
1.00
56.60
B
N


ATOM
6540
CA
ASP
B
258
58.114
7.044
−27.906
1.00
54.48
B
C


ATOM
6541
CB
ASP
B
258
59.108
5.883
−27.776
1.00
45.90
B
C


ATOM
6542
CG
ASP
B
258
59.879
5.897
−26.438
1.00
60.08
B
C


ATOM
6543
OD1
ASP
B
258
60.038
6.974
−25.810
1.00
55.44
B
O


ATOM
6544
OD2
ASP
B
258
60.340
4.813
−26.012
1.00
61.30
B
O


ATOM
6545
C
ASP
B
258
58.855
8.420
−27.908
1.00
53.21
B
C


ATOM
6546
O
ASP
B
258
59.700
8.681
−28.777
1.00
54.31
B
O


ATOM
6547
N
ARG
B
259
58.529
9.285
−26.943
1.00
39.90
B
N


ATOM
6548
CA
AARG
B
259
59.112
10.631
−26.889
0.50
40.52
B
C


ATOM
6549
CA
BARG
B
259
59.083
10.634
−26.879
0.50
40.15
B
C


ATOM
6550
CB
AARG
B
259
58.062
11.680
−27.306
0.50
43.68
B
C


ATOM
6551
CB
BARG
B
259
57.973
11.635
−27.224
0.50
42.16
B
C


ATOM
6552
CG
AARG
B
259
57.732
11.710
−28.815
0.50
51.56
B
C


ATOM
6553
CG
BARG
B
259
57.503
11.561
−28.680
0.50
50.00
B
C


ATOM
6554
CD
AARG
B
259
58.823
12.403
−29.649
0.50
50.35
B
C


ATOM
6555
CD
BARG
B
259
58.131
12.661
−29.517
0.50
47.88
B
C


ATOM
6556
NE
AARG
B
259
58.527
12.394
−31.084
0.50
46.77
B
N


ATOM
6557
NE
BARG
B
259
57.393
13.904
−29.360
0.50
46.89
B
N


ATOM
6558
CZ
AARG
B
259
58.843
11.397
−31.910
0.50
41.60
B
C


ATOM
6559
CZ
BARG
B
259
57.948
15.108
−29.313
0.50
43.09
B
C


ATOM
6560
NH1
AARG
B
259
59.478
10.330
−31.449
0.50
40.52
B
N


ATOM
6561
NH1
BARG
B
259
59.259
15.257
−29.410
0.50
39.41
B
N


ATOM
6562
NH2
AARG
B
259
58.525
11.464
−33.195
0.50
37.19
B
N


ATOM
6563
NH2
BARG
B
259
57.177
16.165
−29.165
0.50
35.24
B
N


ATOM
6564
C
ARG
B
259
59.665
10.943
−25.492
1.00
36.58
B
C


ATOM
6565
O
ARG
B
259
59.645
12.110
−25.032
1.00
28.62
B
O


ATOM
6566
N
LEU
B
260
60.177
9.928
−24.805
1.00
31.08
B
N


ATOM
6567
CA
LEU
B
260
60.619
10.149
−23.406
1.00
34.96
B
C


ATOM
6568
CB
LEU
B
260
61.009
8.858
−22.674
1.00
37.76
B
C


ATOM
6569
CG
LEU
B
260
61.451
9.053
−21.202
1.00
35.28
B
C


ATOM
6570
CD1
LEU
B
260
60.369
9.711
−20.327
1.00
43.36
B
C


ATOM
6571
CD2
LEU
B
260
61.853
7.776
−20.575
1.00
46.40
B
C


ATOM
6572
C
LEU
B
260
61.767
11.133
−23.264
1.00
36.19
B
C


ATOM
6573
O
LEU
B
260
61.702
12.034
−22.412
1.00
32.69
B
O


ATOM
6574
N
ILE
B
261
62.821
10.955
−24.064
1.00
31.53
B
N


ATOM
6575
CA
ILE
B
261
64.025
11.787
−23.909
1.00
30.92
B
C


ATOM
6576
CB
ILE
B
261
65.243
11.293
−24.781
1.00
30.88
B
C


ATOM
6577
CG1
ILE
B
261
65.698
9.894
−24.338
1.00
39.52
B
C


ATOM
6578
CD1
ILE
B
261
66.531
9.099
−25.417
1.00
39.51
B
C


ATOM
6579
CG2
ILE
B
261
66.433
12.250
−24.639
1.00
33.59
B
C


ATOM
6580
C
ILE
B
261
63.633
13.247
−24.202
1.00
21.15
B
C


ATOM
6581
O
ILE
B
261
63.937
14.128
−23.402
1.00
24.35
B
O


ATOM
6582
N
GLU
B
262
62.893
13.466
−25.294
1.00
23.43
B
N


ATOM
6583
CA
GLU
B
262
62.398
14.791
−25.667
1.00
29.00
B
C


ATOM
6584
CB
GLU
B
262
61.608
14.772
−26.987
1.00
37.83
B
C


ATOM
6585
CG
GLU
B
262
62.406
14.298
−28.231
1.00
50.13
B
C


ATOM
6586
CD
GLU
B
262
62.232
12.788
−28.595
1.00
58.78
B
C


ATOM
6587
OE1
GLU
B
262
62.453
11.867
−27.752
1.00
43.65
B
O


ATOM
6588
OE2
GLU
B
262
61.903
12.525
−29.767
1.00
64.04
B
O


ATOM
6589
C
GLU
B
262
61.533
15.402
−24.562
1.00
25.19
B
C


ATOM
6590
O
GLU
B
262
61.622
16.598
−24.319
1.00
24.01
B
O


ATOM
6591
N
SER
B
263
60.719
14.584
−23.884
1.00
24.94
B
N


ATOM
6592
CA
ASER
B
263
59.826
15.026
−22.799
0.50
20.12
B
C


ATOM
6593
CA
BSER
B
263
59.830
15.116
−22.868
0.50
21.08
B
C


ATOM
6594
CB
ASER
B
263
58.970
13.859
−22.267
0.50
19.81
B
C


ATOM
6595
CB
BSER
B
263
58.731
14.108
−22.525
0.50
21.88
B
C


ATOM
6596
OG
ASER
B
263
59.731
12.961
−21.442
0.50
18.14
B
O


ATOM
6597
OG
BSER
B
263
57.782
14.044
−23.590
0.50
25.34
B
O


ATOM
6598
C
SER
B
263
60.664
15.570
−21.651
1.00
22.12
B
C


ATOM
6599
O
SER
B
263
60.340
16.582
−21.015
1.00
18.32
B
O


ATOM
6600
N
PHE
B
264
61.750
14.885
−21.379
1.00
21.21
B
N


ATOM
6601
CA
PHE
B
264
62.627
15.295
−20.301
1.00
21.14
B
C


ATOM
6602
CB
PHE
B
264
63.533
14.152
−19.848
1.00
25.11
B
C


ATOM
6603
CG
PHE
B
264
64.021
14.362
−18.483
1.00
21.53
B
C


ATOM
6604
CD1
PHE
B
264
63.229
14.001
−17.411
1.00
26.88
B
C


ATOM
6605
CE1
PHE
B
264
63.615
14.266
−16.116
1.00
32.05
B
C


ATOM
6606
CZ
PHE
B
264
64.803
14.957
−15.886
1.00
30.00
B
C


ATOM
6607
CE2
PHE
B
264
65.579
15.347
−16.948
1.00
26.11
B
C


ATOM
6608
CD2
PHE
B
264
65.161
15.075
−18.256
1.00
29.07
B
C


ATOM
6609
C
PHE
B
264
63.428
16.566
−20.598
1.00
20.61
B
C


ATOM
6610
O
PHE
B
264
63.547
17.464
−19.744
1.00
20.73
B
O


ATOM
6611
N
TYR
B
265
63.946
16.665
−21.815
1.00
20.09
B
N


ATOM
6612
CA
TYR
B
265
64.607
17.881
−22.298
1.00
17.15
B
C


ATOM
6613
CB
TYR
B
265
65.040
17.662
−23.748
1.00
19.86
B
C


ATOM
6614
CG
TYR
B
265
65.504
18.895
−24.472
1.00
23.47
B
C


ATOM
6615
CD1
TYR
B
265
66.800
19.361
−24.310
1.00
31.92
B
C


ATOM
6616
CE1
TYR
B
265
67.244
20.493
−24.966
1.00
30.60
B
C


ATOM
6617
CZ
TYR
B
265
66.400
21.181
−25.794
1.00
34.74
B
C


ATOM
6618
OH
TYR
B
265
66.885
22.293
−26.426
1.00
38.69
B
O


ATOM
6619
CE2
TYR
B
265
65.092
20.737
−25.993
1.00
32.63
B
C


ATOM
6620
CD2
TYR
B
265
64.659
19.590
−25.330
1.00
24.71
B
C


ATOM
6621
C
TYR
B
265
63.665
19.074
−22.169
1.00
18.92
B
C


ATOM
6622
O
TYR
B
265
64.051
20.158
−21.734
1.00
18.09
B
O


ATOM
6623
N
TRP
B
266
62.409
18.853
−22.526
1.00
16.41
B
N


ATOM
6624
CA
TRP
B
266
61.377
19.870
−22.400
1.00
18.68
B
C


ATOM
6625
CB
TRP
B
266
60.081
19.335
−23.011
1.00
19.17
B
C


ATOM
6626
CG
TRP
B
266
58.915
20.266
−22.847
1.00
19.86
B
C


ATOM
6627
CD1
TRP
B
266
58.060
20.383
−21.776
1.00
20.93
B
C


ATOM
6628
NE1
TRP
B
266
57.139
21.348
−22.002
1.00
20.77
B
N


ATOM
6629
CE2
TRP
B
266
57.358
21.940
−23.183
1.00
20.01
B
C


ATOM
6630
CD2
TRP
B
266
58.475
21.268
−23.797
1.00
16.68
B
C


ATOM
6631
CE3
TRP
B
266
58.902
21.664
−25.057
1.00
19.39
B
C


ATOM
6632
CZ3
TRP
B
266
58.218
22.692
−25.686
1.00
20.94
B
C


ATOM
6633
CH2
TRP
B
266
57.136
23.310
−25.085
1.00
20.17
B
C


ATOM
6634
CZ2
TRP
B
266
56.677
22.941
−23.824
1.00
25.72
B
C


ATOM
6635
C
TRP
B
266
61.225
20.265
−20.965
1.00
17.64
B
C


ATOM
6636
O
TRP
B
266
61.259
21.454
−20.624
1.00
15.67
B
O


ATOM
6637
N
ALA
B
267
61.101
19.272
−20.079
1.00
18.06
B
N


ATOM
6638
CA
ALA
B
267
60.975
19.552
−18.649
1.00
20.03
B
C


ATOM
6639
CB
ALA
B
267
60.791
18.268
−17.842
1.00
22.95
B
C


ATOM
6640
C
ALA
B
267
62.131
20.366
−18.054
1.00
17.74
B
C


ATOM
6641
O
ALA
B
267
61.905
21.206
−17.172
1.00
16.57
B
O


ATOM
6642
N
VAL
B
268
63.347
20.114
−18.522
1.00
17.36
B
N


ATOM
6643
CA
VAL
B
268
64.519
20.826
−18.021
1.00
15.22
B
C


ATOM
6644
CB
VAL
B
268
65.836
20.263
−18.636
1.00
16.11
B
C


ATOM
6645
CG1
VAL
B
268
67.029
21.251
−18.463
1.00
14.19
B
C


ATOM
6646
CG2
VAL
B
268
66.188
18.882
−18.025
1.00
18.11
B
C


ATOM
6647
C
VAL
B
268
64.381
22.342
−18.282
1.00
20.16
B
C


ATOM
6648
O
VAL
B
268
64.812
23.166
−17.472
1.00
17.44
B
O


ATOM
6649
N
GLY
B
269
63.760
22.699
−19.406
1.00
18.75
B
N


ATOM
6650
CA
GLY
B
269
63.566
24.128
−19.765
1.00
18.20
B
C


ATOM
6651
C
GLY
B
269
62.541
24.803
−18.882
1.00
18.02
B
C


ATOM
6652
O
GLY
B
269
62.657
25.990
−18.590
1.00
18.41
B
O


ATOM
6653
N
VAL
B
270
61.532
24.038
−18.447
1.00
18.51
B
N


ATOM
6654
CA
VAL
B
270
60.502
24.543
−17.550
1.00
17.42
B
C


ATOM
6655
CB
VAL
B
270
59.273
23.618
−17.547
1.00
17.32
B
C


ATOM
6656
CG1
VAL
B
270
58.273
24.056
−16.526
1.00
15.84
B
C


ATOM
6657
CG2
VAL
B
270
58.650
23.582
−19.014
1.00
17.61
B
C


ATOM
6658
C
VAL
B
270
61.038
24.725
−16.121
1.00
19.45
B
C


ATOM
6659
O
VAL
B
270
60.746
25.732
−15.473
1.00
17.68
B
O


ATOM
6660
N
ALA
B
271
61.799
23.751
−15.618
1.00
15.53
B
N


ATOM
6661
CA
ALA
B
271
62.329
23.853
−14.252
1.00
18.34
B
C


ATOM
6662
CB
ALA
B
271
61.370
23.161
−13.275
1.00
20.38
B
C


ATOM
6663
C
ALA
B
271
63.734
23.255
−14.171
1.00
21.27
B
C


ATOM
6664
O
ALA
B
271
63.903
22.013
−14.018
1.00
19.31
B
O


ATOM
6665
N
PHE
B
272
64.753
24.102
−14.304
1.00
15.12
B
N


ATOM
6666
CA
PHE
B
272
66.125
23.605
−14.456
1.00
17.09
B
C


ATOM
6667
CB
PHE
B
272
66.958
24.540
−15.326
1.00
19.46
B
C


ATOM
6668
CG
PHE
B
272
67.462
25.746
−14.582
1.00
25.15
B
C


ATOM
6669
CD1
PHE
B
272
68.674
25.709
−13.922
1.00
31.93
B
C


ATOM
6670
CE1
PHE
B
272
69.121
26.832
−13.211
1.00
34.44
B
C


ATOM
6671
CZ
PHE
B
272
68.344
27.985
−13.178
1.00
27.74
B
C


ATOM
6672
CE2
PHE
B
272
67.148
28.022
−13.836
1.00
36.19
B
C


ATOM
6673
CD2
PHE
B
272
66.701
26.896
−14.523
1.00
31.96
B
C


ATOM
6674
C
PHE
B
272
66.872
23.409
−13.128
1.00
19.83
B
C


ATOM
6675
O
PHE
B
272
67.880
22.670
−13.108
1.00
19.24
B
O


ATOM
6676
N
GLU
B
273
66.433
24.104
−12.070
1.00
19.54
B
N


ATOM
6677
CA
GLU
B
273
67.218
24.156
−10.822
1.00
25.70
B
C


ATOM
6678
CB
GLU
B
273
66.643
25.121
−9.795
1.00
23.52
B
C


ATOM
6679
CG
GLU
B
273
66.692
26.586
−10.212
1.00
29.09
B
C


ATOM
6680
CD
GLU
B
273
65.428
27.067
−10.970
1.00
37.43
B
C


ATOM
6681
OE1
GLU
B
273
64.703
26.283
−11.672
1.00
32.92
B
O


ATOM
6682
OE2
GLU
B
273
65.168
28.280
−10.865
1.00
42.12
B
O


ATOM
6683
C
GLU
B
273
67.269
22.747
−10.233
1.00
26.94
B
C


ATOM
6684
O
GLU
B
273
66.280
22.009
−10.309
1.00
22.77
B
O


ATOM
6685
N
PRO
B
274
68.412
22.370
−9.645
1.00
24.26
B
N


ATOM
6686
CA
PRO
B
274
68.579
20.985
−9.233
1.00
25.14
B
C


ATOM
6687
CB
PRO
B
274
69.980
20.957
−8.589
1.00
27.41
B
C


ATOM
6688
CG
PRO
B
274
70.373
22.350
−8.413
1.00
27.95
B
C


ATOM
6689
CD
PRO
B
274
69.630
23.165
−9.418
1.00
25.84
B
C


ATOM
6690
C
PRO
B
274
67.504
20.495
−8.265
1.00
24.47
B
C


ATOM
6691
O
PRO
B
274
67.119
19.342
−8.326
1.00
27.78
B
O


ATOM
6692
N
GLN
B
275
66.958
21.376
−7.448
1.00
23.27
B
N


ATOM
6693
CA
GLN
B
275
65.948
20.961
−6.466
1.00
26.52
B
C


ATOM
6694
CB
GLN
B
275
65.773
22.025
−5.373
1.00
25.42
B
C


ATOM
6695
CG
GLN
B
275
65.269
23.415
−5.793
1.00
28.04
B
C


ATOM
6696
CD
GLN
B
275
66.375
24.384
−6.239
1.00
33.28
B
C


ATOM
6697
OE1
GLN
B
275
67.456
23.974
−6.698
1.00
29.99
B
O


ATOM
6698
NE2
GLN
B
275
66.101
25.689
−6.110
1.00
33.44
B
N


ATOM
6699
C
GLN
B
275
64.566
20.626
−7.038
1.00
25.38
B
C


ATOM
6700
O
GLN
B
275
63.718
20.145
−6.298
1.00
28.33
B
O


ATOM
6701
N
TYR
B
276
64.327
20.886
−8.330
1.00
24.88
B
N


ATOM
6702
CA
TYR
B
276
62.980
20.695
−8.935
1.00
22.85
B
C


ATOM
6703
CB
TYR
B
276
62.654
21.874
−9.882
1.00
21.39
B
C


ATOM
6704
CG
TYR
B
276
62.398
23.193
−9.161
1.00
27.30
B
C


ATOM
6705
CD1
TYR
B
276
61.533
23.261
−8.066
1.00
29.89
B
C


ATOM
6706
CE1
TYR
B
276
61.304
24.472
−7.396
1.00
31.01
B
C


ATOM
6707
CZ
TYR
B
276
61.929
25.618
−7.831
1.00
36.98
B
C


ATOM
6708
OH
TYR
B
276
61.697
26.789
−7.167
1.00
41.65
B
O


ATOM
6709
CE2
TYR
B
276
62.773
25.586
−8.919
1.00
36.74
B
C


ATOM
6710
CD2
TYR
B
276
63.007
24.366
−9.580
1.00
31.75
B
C


ATOM
6711
C
TYR
B
276
62.810
19.374
−9.682
1.00
25.69
B
C


ATOM
6712
O
TYR
B
276
62.140
19.320
−10.698
1.00
18.56
B
O


ATOM
6713
N
SER
B
277
63.400
18.315
−9.156
1.00
20.78
B
N


ATOM
6714
CA
SER
B
277
63.267
16.995
−9.690
1.00
20.77
B
C


ATOM
6715
CB
SER
B
277
64.023
16.001
−8.789
1.00
28.26
B
C


ATOM
6716
OG
SER
B
277
65.369
15.907
−9.232
1.00
32.73
B
O


ATOM
6717
C
SER
B
277
61.837
16.574
−9.856
1.00
20.62
B
C


ATOM
6718
O
SER
B
277
61.491
16.016
−10.904
1.00
20.18
B
O


ATOM
6719
N
ASP
B
278
61.001
16.838
−8.855
1.00
19.70
B
N


ATOM
6720
CA
ASP
B
278
59.601
16.441
−8.904
1.00
21.39
B
C


ATOM
6721
CB
ASP
B
278
58.881
16.772
−7.615
1.00
27.49
B
C


ATOM
6722
CG
ASP
B
278
59.286
15.867
−6.481
1.00
35.28
B
C


ATOM
6723
OD1
ASP
B
278
60.058
14.915
−6.727
1.00
45.25
B
O


ATOM
6724
OD2
ASP
B
278
58.838
16.116
−5.344
1.00
49.36
B
O


ATOM
6725
C
ASP
B
278
58.885
17.184
−10.012
1.00
24.04
B
C


ATOM
6726
O
ASP
B
278
58.095
16.584
−10.728
1.00
23.33
B
O


ATOM
6727
N
CYS
B
279
59.148
18.491
−10.129
1.00
19.17
B
N


ATOM
6728
CA
CYS
B
279
58.561
19.249
−11.208
1.00
21.15
B
C


ATOM
6729
CB
CYS
B
279
58.962
20.740
−11.171
1.00
20.42
B
C


ATOM
6730
SG
CYS
B
279
57.972
21.692
−12.360
1.00
23.47
B
S


ATOM
6731
C
CYS
B
279
58.942
18.625
−12.531
1.00
19.45
B
C


ATOM
6732
O
CYS
B
279
58.079
18.409
−13.379
1.00
20.55
B
O


ATOM
6733
N
ARG
B
280
60.229
18.352
−12.740
1.00
15.98
B
N


ATOM
6734
CA
ARG
B
280
60.655
17.808
−14.028
1.00
18.83
B
C


ATOM
6735
CB
ARG
B
280
62.175
17.634
−14.116
1.00
17.83
B
C


ATOM
6736
CG
ARG
B
280
62.977
18.950
−14.224
1.00
19.13
B
C


ATOM
6737
CD
ARG
B
280
64.438
18.697
−14.456
1.00
19.75
B
C


ATOM
6738
NE
ARG
B
280
65.103
18.174
−13.272
1.00
16.55
B
N


ATOM
6739
CZ
ARG
B
280
65.525
18.928
−12.252
1.00
18.61
B
C


ATOM
6740
NH1
ARG
B
280
65.329
20.238
−12.274
1.00
17.30
B
N


ATOM
6741
NH2
ARG
B
280
66.148
18.371
−11.211
1.00
19.56
B
N


ATOM
6742
C
ARG
B
280
60.040
16.454
−14.320
1.00
21.11
B
C


ATOM
6743
O
ARG
B
280
59.685
16.164
−15.467
1.00
18.91
B
O


ATOM
6744
N
ASN
B
281
59.990
15.610
−13.300
1.00
19.83
B
N


ATOM
6745
CA
ASN
B
281
59.389
14.284
−13.429
1.00
20.75
B
C


ATOM
6746
CB
ASN
B
281
59.656
13.442
−12.196
1.00
24.86
B
C


ATOM
6747
CG
ASN
B
281
61.119
13.072
−12.056
1.00
35.79
B
C


ATOM
6748
OD1
ASN
B
281
61.902
13.110
−13.030
1.00
38.79
B
O


ATOM
6749
ND2
ASN
B
281
61.500
12.696
−10.843
1.00
31.24
B
N


ATOM
6750
C
ASN
B
281
57.899
14.329
−13.758
1.00
17.43
B
C


ATOM
6751
O
ASN
B
281
57.465
13.643
−14.687
1.00
20.51
B
O


ATOM
6752
N
SER
B
282
57.136
15.163
−13.061
1.00
20.69
B
N


ATOM
6753
CA
SER
B
282
55.736
15.391
−13.391
1.00
22.52
B
C


ATOM
6754
CB
SER
B
282
55.156
16.453
−12.473
1.00
29.52
B
C


ATOM
6755
OG
SER
B
282
54.323
15.891
−11.515
1.00
36.99
B
O


ATOM
6756
C
SER
B
282
55.522
15.905
−14.818
1.00
21.80
B
C


ATOM
6757
O
SER
B
282
54.669
15.420
−15.565
1.00
19.88
B
O


ATOM
6758
N
VAL
B
283
56.272
16.932
−15.170
1.00
18.27
B
N


ATOM
6759
CA
VAL
B
283
56.138
17.518
−16.473
1.00
16.31
B
C


ATOM
6760
CB
VAL
B
283
56.903
18.849
−16.607
1.00
18.31
B
C


ATOM
6761
CG1
VAL
B
283
56.910
19.316
−18.088
1.00
15.14
B
C


ATOM
6762
CG2
VAL
B
283
56.253
19.885
−15.718
1.00
18.23
B
C


ATOM
6763
C
VAL
B
283
56.497
16.537
−17.557
1.00
19.38
B
C


ATOM
6764
O
VAL
B
283
55.760
16.445
−18.546
1.00
15.39
B
O


ATOM
6765
N
ALA
B
284
57.586
15.773
−17.380
1.00
17.29
B
N


ATOM
6766
CA
ALA
B
284
57.987
14.781
−18.383
1.00
17.24
B
C


ATOM
6767
CB
ALA
B
284
59.375
14.198
−18.029
1.00
18.23
B
C


ATOM
6768
C
ALA
B
284
56.940
13.662
−18.528
1.00
17.47
B
C


ATOM
6769
O
ALA
B
284
56.590
13.217
−19.657
1.00
16.74
B
O


ATOM
6770
N
LYS
B
285
56.436
13.175
−17.399
1.00
16.38
B
N


ATOM
6771
CA
LYS
B
285
55.369
12.155
−17.427
1.00
15.42
B
C


ATOM
6772
CB
LYS
B
285
54.982
11.727
−16.010
1.00
15.54
B
C


ATOM
6773
CG
LYS
B
285
56.019
10.886
−15.343
1.00
20.92
B
C


ATOM
6774
CD
LYS
B
285
55.558
10.490
−13.930
1.00
26.64
B
C


ATOM
6775
CE
LYS
B
285
56.471
9.484
−13.263
1.00
30.58
B
C


ATOM
6776
NZ
LYS
B
285
56.066
9.445
−11.827
1.00
42.27
B
N


ATOM
6777
C
LYS
B
285
54.139
12.664
−18.182
1.00
15.13
B
C


ATOM
6778
O
LYS
B
285
53.560
11.958
−19.018
1.00
16.43
B
O


ATOM
6779
N
MET
B
286
53.739
13.889
−17.904
1.00
18.03
B
N


ATOM
6780
CA
MET
B
286
52.537
14.415
−18.534
1.00
19.05
B
C


ATOM
6781
CB
MET
B
286
52.053
15.724
−17.936
1.00
19.10
B
C


ATOM
6782
CG
MET
B
286
51.543
15.612
−16.503
1.00
19.96
B
C


ATOM
6783
SD
MET
B
286
50.293
14.375
−16.265
1.00
25.05
B
S


ATOM
6784
CE
MET
B
286
51.185
12.968
−15.611
1.00
23.35
B
C


ATOM
6785
C
MET
B
286
52.794
14.590
−20.012
1.00
20.13
B
C


ATOM
6786
O
MET
B
286
51.932
14.265
−20.823
1.00
20.15
B
O


ATOM
6787
N
PHE
B
287
53.981
15.051
−20.374
1.00
17.26
B
N


ATOM
6788
CA
PHE
B
287
54.260
15.220
−21.806
1.00
17.95
B
C


ATOM
6789
CB
PHE
B
287
55.548
15.984
−22.074
1.00
28.34
B
C


ATOM
6790
CG
PHE
B
287
55.446
16.967
−23.231
1.00
44.47
B
C


ATOM
6791
CD1
PHE
B
287
54.194
17.252
−23.856
1.00
56.86
B
C


ATOM
6792
CE1
PHE
B
287
54.081
18.161
−24.926
1.00
41.77
B
C


ATOM
6793
CZ
PHE
B
287
55.210
18.832
−25.367
1.00
45.38
B
C


ATOM
6794
CE2
PHE
B
287
56.470
18.563
−24.755
1.00
57.85
B
C


ATOM
6795
CD2
PHE
B
287
56.576
17.637
−23.688
1.00
41.58
B
C


ATOM
6796
C
PHE
B
287
54.249
13.922
−22.558
1.00
16.03
B
C


ATOM
6797
O
PHE
B
287
53.789
13.874
−23.715
1.00
15.83
B
O


ATOM
6798
N
CYS
B
288
54.685
12.847
−21.909
1.00
14.89
B
N


ATOM
6799
CA
CYS
B
288
54.586
11.519
−22.509
1.00
14.98
B
C


ATOM
6800
CB
CYS
B
288
55.247
10.449
−21.636
1.00
21.40
B
C


ATOM
6801
SG
CYS
B
288
57.050
10.528
−21.550
1.00
25.90
B
S


ATOM
6802
C
CYS
B
288
53.150
11.096
−22.809
1.00
18.56
B
C


ATOM
6803
O
CYS
B
288
52.843
10.623
−23.899
1.00
17.61
B
O


ATOM
6804
N
PHE
B
289
52.272
11.229
−21.827
1.00
15.35
B
N


ATOM
6805
CA
PHE
B
289
50.857
10.959
−22.052
1.00
16.35
B
C


ATOM
6806
CB
PHE
B
289
50.088
10.988
−20.705
1.00
15.27
B
C


ATOM
6807
CG
PHE
B
289
50.203
9.706
−19.972
1.00
17.14
B
C


ATOM
6808
CD1
PHE
B
289
49.626
8.573
−20.486
1.00
20.13
B
C


ATOM
6809
CE1
PHE
B
289
49.783
7.349
−19.837
1.00
21.56
B
C


ATOM
6810
CZ
PHE
B
289
50.523
7.270
−18.699
1.00
18.36
B
C


ATOM
6811
CE2
PHE
B
289
51.122
8.381
−18.197
1.00
20.87
B
C


ATOM
6812
CD2
PHE
B
289
50.984
9.594
−18.830
1.00
23.02
B
C


ATOM
6813
C
PHE
B
289
50.211
11.900
−23.043
1.00
15.41
B
C


ATOM
6814
O
PHE
B
289
49.451
11.459
−23.898
1.00
17.63
B
O


ATOM
6815
N
VAL
B
290
50.525
13.195
−22.955
1.00
15.98
B
N


ATOM
6816
CA
VAL
B
290
49.986
14.129
−23.933
1.00
15.11
B
C


ATOM
6817
CB
VAL
B
290
50.467
15.588
−23.701
1.00
16.79
B
C


ATOM
6818
CG1
VAL
B
290
50.133
16.477
−24.909
1.00
15.60
B
C


ATOM
6819
CG2
VAL
B
290
49.837
16.138
−22.433
1.00
13.84
B
C


ATOM
6820
C
VAL
B
290
50.294
13.682
−25.341
1.00
16.81
B
C


ATOM
6821
O
VAL
B
290
49.416
13.687
−26.193
1.00
17.52
B
O


ATOM
6822
N
THR
B
291
51.529
13.294
−25.601
1.00
19.53
B
N


ATOM
6823
CA
THR
B
291
51.912
12.949
−26.980
1.00
22.80
B
C


ATOM
6824
CB
THR
B
291
53.428
12.631
−27.106
1.00
25.06
B
C


ATOM
6825
OG1
THR
B
291
53.723
11.394
−26.472
1.00
44.14
B
O


ATOM
6826
CG2
THR
B
291
54.302
13.691
−26.473
1.00
19.46
B
C


ATOM
6827
C
THR
B
291
51.128
11.733
−27.510
1.00
23.54
B
C


ATOM
6828
O
THR
B
291
50.706
11.710
−28.669
1.00
20.69
B
O


ATOM
6829
N
ILE
B
292
50.976
10.707
−26.674
1.00
18.62
B
N


ATOM
6830
CA
ILE
B
292
50.199
9.511
−27.040
1.00
17.16
B
C


ATOM
6831
CB
ILE
B
292
50.337
8.423
−25.941
1.00
20.53
B
C


ATOM
6832
CG1
ILE
B
292
51.780
7.968
−25.861
1.00
27.07
B
C


ATOM
6833
CD1
ILE
B
292
52.015
6.951
−24.785
1.00
33.53
B
C


ATOM
6834
CG2
ILE
B
292
49.424
7.239
−26.257
1.00
23.47
B
C


ATOM
6835
C
ILE
B
292
48.708
9.796
−27.207
1.00
13.31
B
C


ATOM
6836
O
ILE
B
292
48.090
9.376
−28.172
1.00
15.77
B
O


ATOM
6837
N
ILE
B
293
48.126
10.512
−26.269
1.00
13.87
B
N


ATOM
6838
CA
ILE
B
293
46.708
10.848
−26.334
1.00
14.79
B
C


ATOM
6839
CB
ILE
B
293
46.218
11.378
−24.992
1.00
14.65
B
C


ATOM
6840
CG1
ILE
B
293
46.253
10.221
−23.968
1.00
19.35
B
C


ATOM
6841
CD1
ILE
B
293
46.061
10.663
−22.555
1.00
20.43
B
C


ATOM
6842
CG2
ILE
B
293
44.751
11.910
−25.141
1.00
14.75
B
C


ATOM
6843
C
ILE
B
293
46.379
11.819
−27.489
1.00
18.92
B
C


ATOM
6844
O
ILE
B
293
45.380
11.641
−28.221
1.00
16.07
B
O


ATOM
6845
N
ASP
B
294
47.265
12.767
−27.720
1.00
17.62
B
N


ATOM
6846
CA
ASP
B
294
47.146
13.669
−28.911
1.00
20.00
B
C


ATOM
6847
CB
ASP
B
294
48.345
14.612
−28.967
1.00
25.16
B
C


ATOM
6848
CG
ASP
B
294
48.176
15.781
−29.984
1.00
36.99
B
C


ATOM
6849
OD1
ASP
B
294
47.380
15.706
−30.906
1.00
37.88
B
O


ATOM
6850
OD2
ASP
B
294
48.880
16.800
−29.836
1.00
58.01
B
O


ATOM
6851
C
ASP
B
294
47.098
12.826
−30.188
1.00
18.94
B
C


ATOM
6852
O
ASP
B
294
46.244
13.039
−31.021
1.00
16.12
B
O


ATOM
6853
N
ASP
B
295
47.997
11.861
−30.327
1.00
18.01
B
N


ATOM
6854
CA
ASP
B
295
47.973
10.931
−31.468
1.00
20.14
B
C


ATOM
6855
CB
ASP
B
295
49.096
9.911
−31.363
1.00
27.52
B
C


ATOM
6856
CG
ASP
B
295
50.486
10.484
−31.700
1.00
44.28
B
C


ATOM
6857
OD1
ASP
B
295
50.547
11.583
−32.293
1.00
40.05
B
O


ATOM
6858
OD2
ASP
B
295
51.515
9.815
−31.359
1.00
41.64
B
O


ATOM
6859
C
ASP
B
295
46.634
10.158
−31.640
1.00
21.15
B
C


ATOM
6860
O
ASP
B
295
46.148
9.966
−32.777
1.00
19.75
B
O


ATOM
6861
N
ILE
B
296
46.075
9.679
−30.532
1.00
16.24
B
N


ATOM
6862
CA
ILE
B
296
44.775
8.990
−30.554
1.00
15.66
B
C


ATOM
6863
CB
ILE
B
296
44.413
8.461
−29.122
1.00
16.02
B
C


ATOM
6864
CG1
ILE
B
296
45.371
7.284
−28.781
1.00
18.95
B
C


ATOM
6865
CD1
ILE
B
296
45.180
6.693
−27.375
1.00
17.17
B
C


ATOM
6866
CG2
ILE
B
296
42.958
7.993
−29.072
1.00
14.97
B
C


ATOM
6867
C
ILE
B
296
43.703
9.890
−31.152
1.00
13.43
B
C


ATOM
6868
O
ILE
B
296
43.037
9.517
−32.125
1.00
14.63
B
O


ATOM
6869
N
TYR
B
297
43.568
11.117
−30.624
1.00
15.09
B
N


ATOM
6870
CA
TYR
B
297
42.578
12.057
−31.121
1.00
15.87
B
C


ATOM
6871
CB
TYR
B
297
42.421
13.260
−30.200
1.00
16.95
B
C


ATOM
6872
CG
TYR
B
297
41.637
12.975
−28.960
1.00
16.22
B
C


ATOM
6873
CD1
TYR
B
297
42.233
12.369
−27.876
1.00
18.96
B
C


ATOM
6874
CE1
TYR
B
297
41.533
12.122
−26.734
1.00
19.27
B
C


ATOM
6875
CZ
TYR
B
297
40.226
12.519
−26.653
1.00
16.55
B
C


ATOM
6876
OH
TYR
B
297
39.540
12.273
−25.481
1.00
17.47
B
O


ATOM
6877
CE2
TYR
B
297
39.631
13.175
−27.708
1.00
15.00
B
C


ATOM
6878
CD2
TYR
B
297
40.340
13.379
−28.847
1.00
14.40
B
C


ATOM
6879
C
TYR
B
297
42.881
12.578
−32.516
1.00
17.77
B
C


ATOM
6880
O
TYR
B
297
41.953
12.807
−33.292
1.00
16.97
B
O


ATOM
6881
N
ASP
B
298
44.160
12.742
−32.815
1.00
19.99
B
N


ATOM
6882
CA
ASP
B
298
44.596
13.278
−34.118
1.00
23.26
B
C


ATOM
6883
CB
ASP
B
298
46.045
13.731
−34.047
1.00
28.34
B
C


ATOM
6884
CG
ASP
B
298
46.509
14.437
−35.321
1.00
38.75
B
C


ATOM
6885
OD1
ASP
B
298
45.730
15.246
−35.868
1.00
36.91
B
O


ATOM
6886
OD2
ASP
B
298
47.654
14.182
−35.766
1.00
42.81
B
O


ATOM
6887
C
ASP
B
298
44.408
12.330
−35.296
1.00
23.75
B
C


ATOM
6888
O
ASP
B
298
43.861
12.730
−36.342
1.00
22.43
B
O


ATOM
6889
N
VAL
B
299
44.861
11.077
−35.168
1.00
18.23
B
N


ATOM
6890
CA
VAL
B
299
44.821
10.178
−36.314
1.00
19.25
B
C


ATOM
6891
CB
VAL
B
299
46.219
9.979
−36.949
1.00
24.81
B
C


ATOM
6892
CG1
VAL
B
299
46.692
11.299
−37.581
1.00
29.19
B
C


ATOM
6893
CG2
VAL
B
299
47.221
9.421
−35.934
1.00
22.46
B
C


ATOM
6894
C
VAL
B
299
44.202
8.826
−36.081
1.00
17.35
B
C


ATOM
6895
O
VAL
B
299
43.577
8.296
−36.985
1.00
17.18
B
O


ATOM
6896
N
TYR
B
300
44.364
8.243
−34.899
1.00
16.33
B
N


ATOM
6897
CA
TYR
B
300
43.998
6.828
−34.736
1.00
18.61
B
C


ATOM
6898
CB
TYR
B
300
44.908
6.202
−33.677
1.00
19.79
B
C


ATOM
6899
CG
TYR
B
300
44.840
4.699
−33.651
1.00
19.73
B
C


ATOM
6900
CD1
TYR
B
300
45.633
3.917
−34.507
1.00
18.54
B
C


ATOM
6901
CE1
TYR
B
300
45.557
2.496
−34.474
1.00
19.58
B
C


ATOM
6902
CZ
TYR
B
300
44.654
1.890
−33.597
1.00
17.68
B
C


ATOM
6903
OH
TYR
B
300
44.559
0.552
−33.522
1.00
21.00
B
O


ATOM
6904
CE2
TYR
B
300
43.861
2.640
−32.763
1.00
21.49
B
C


ATOM
6905
CD2
TYR
B
300
43.942
4.049
−32.804
1.00
22.94
B
C


ATOM
6906
C
TYR
B
300
42.500
6.598
−34.396
1.00
15.66
B
C


ATOM
6907
O
TYR
B
300
41.855
5.745
−34.978
1.00
16.10
B
O


ATOM
6908
N
GLY
B
301
41.961
7.349
−33.464
1.00
14.35
B
N


ATOM
6909
CA
GLY
B
301
40.617
7.084
−32.929
1.00
13.88
B
C


ATOM
6910
C
GLY
B
301
39.486
7.560
−33.795
1.00
19.41
B
C


ATOM
6911
O
GLY
B
301
39.578
8.639
−34.396
1.00
17.02
B
O


ATOM
6912
N
THR
B
302
38.415
6.761
−33.889
1.00
17.51
B
N


ATOM
6913
CA
THR
B
302
37.184
7.246
−34.519
1.00
16.58
B
C


ATOM
6914
CB
THR
B
302
36.168
6.117
−34.850
1.00
16.55
B
C


ATOM
6915
OG1
THR
B
302
35.689
5.542
−33.635
1.00
17.13
B
O


ATOM
6916
CG2
THR
B
302
36.770
5.007
−35.748
1.00
12.41
B
C


ATOM
6917
C
THR
B
302
36.489
8.211
−33.581
1.00
16.89
B
C


ATOM
6918
O
THR
B
302
36.696
8.191
−32.392
1.00
17.51
B
O


ATOM
6919
N
LEU
B
303
35.595
9.039
−34.112
1.00
18.82
B
N


ATOM
6920
CA
LEU
B
303
34.924
10.002
−33.280
1.00
18.70
B
C


ATOM
6921
CB
LEU
B
303
34.040
10.902
−34.140
1.00
25.89
B
C


ATOM
6922
CG
LEU
B
303
33.922
12.395
−33.907
1.00
33.97
B
C


ATOM
6923
CD1
LEU
B
303
35.238
13.063
−33.530
1.00
26.30
B
C


ATOM
6924
CD2
LEU
B
303
33.287
13.024
−35.186
1.00
29.85
B
C


ATOM
6925
C
LEU
B
303
34.112
9.354
−32.156
1.00
18.45
B
C


ATOM
6926
O
LEU
B
303
34.101
9.852
−31.031
1.00
18.10
B
O


ATOM
6927
N
ASP
B
304
33.454
8.233
−32.441
1.00
18.11
B
N


ATOM
6928
CA
ASP
B
304
32.737
7.445
−31.405
1.00
19.14
B
C


ATOM
6929
CB
ASP
B
304
32.027
6.217
−32.020
1.00
24.92
B
C


ATOM
6930
CG
ASP
B
304
30.770
6.589
−32.888
1.00
37.62
B
C


ATOM
6931
OD1
ASP
B
304
30.233
7.725
−32.793
1.00
36.17
B
O


ATOM
6932
OD2
ASP
B
304
30.316
5.728
−33.674
1.00
42.17
B
O


ATOM
6933
C
ASP
B
304
33.674
6.976
−30.264
1.00
17.27
B
C


ATOM
6934
O
ASP
B
304
33.298
7.015
−29.098
1.00
15.49
B
O


ATOM
6935
N
GLU
B
305
34.883
6.536
−30.610
1.00
17.49
B
N


ATOM
6936
CA
GLU
B
305
35.858
6.090
−29.613
1.00
15.60
B
C


ATOM
6937
CB
GLU
B
305
37.055
5.401
−30.301
1.00
16.67
B
C


ATOM
6938
CG
GLU
B
305
36.732
4.136
−31.078
1.00
17.92
B
C


ATOM
6939
CD
GLU
B
305
37.954
3.572
−31.821
1.00
18.92
B
C


ATOM
6940
OE1
GLU
B
305
38.882
4.342
−32.205
1.00
17.92
B
O


ATOM
6941
OE2
GLU
B
305
38.046
2.336
−31.953
1.00
19.32
B
O


ATOM
6942
C
GLU
B
305
36.352
7.284
−28.803
1.00
14.90
B
C


ATOM
6943
O
GLU
B
305
36.552
7.186
−27.608
1.00
14.85
B
O


ATOM
6944
N
LEU
B
306
36.560
8.417
−29.476
1.00
14.91
B
N


ATOM
6945
CA
LEU
B
306
37.066
9.604
−28.826
1.00
13.56
B
C


ATOM
6946
CB
LEU
B
306
37.460
10.651
−29.876
1.00
13.42
B
C


ATOM
6947
CG
LEU
B
306
38.586
10.284
−30.878
1.00
14.82
B
C


ATOM
6948
CD1
LEU
B
306
38.847
11.573
−31.746
1.00
14.84
B
C


ATOM
6949
CD2
LEU
B
306
39.834
9.840
−30.171
1.00
15.93
B
C


ATOM
6950
C
LEU
B
306
36.048
10.161
−27.816
1.00
13.30
B
C


ATOM
6951
O
LEU
B
306
36.411
10.666
−26.751
1.00
14.09
B
O


ATOM
6952
N
GLU
B
307
34.762
10.034
−28.125
1.00
14.71
B
N


ATOM
6953
CA
GLU
B
307
33.729
10.442
−27.179
1.00
16.27
B
C


ATOM
6954
CB
GLU
B
307
32.331
10.356
−27.807
1.00
23.38
B
C


ATOM
6955
CG
GLU
B
307
32.111
11.353
−28.928
1.00
26.61
B
C


ATOM
6956
CD
GLU
B
307
31.648
12.688
−28.409
1.00
37.36
B
C


ATOM
6957
OE1
GLU
B
307
31.531
12.827
−27.163
1.00
34.44
B
O


ATOM
6958
OE2
GLU
B
307
31.397
13.588
−29.240
1.00
39.03
B
O


ATOM
6959
C
GLU
B
307
33.763
9.556
−25.955
1.00
17.30
B
C


ATOM
6960
O
GLU
B
307
33.624
10.048
−24.860
1.00
16.25
B
O


ATOM
6961
N
LEU
B
308
33.958
8.246
−26.136
1.00
17.52
B
N


ATOM
6962
CA
LEU
B
308
34.075
7.326
−24.986
1.00
20.67
B
C


ATOM
6963
CB
LEU
B
308
34.267
5.863
−25.451
1.00
23.47
B
C


ATOM
6964
CG
LEU
B
308
33.023
5.193
−26.041
1.00
25.34
B
C


ATOM
6965
CD1
LEU
B
308
33.350
3.738
−26.465
1.00
23.64
B
C


ATOM
6966
CD2
LEU
B
308
31.862
5.193
−25.028
1.00
27.52
B
C


ATOM
6967
C
LEU
B
308
35.240
7.674
−24.111
1.00
17.44
B
C


ATOM
6968
O
LEU
B
308
35.124
7.703
−22.887
1.00
15.08
B
O


ATOM
6969
N
PHE
B
309
36.386
7.934
−24.736
1.00
15.50
B
N


ATOM
6970
CA
PHE
B
309
37.597
8.288
−23.969
1.00
14.42
B
C


ATOM
6971
CB
PHE
B
309
38.816
8.407
−24.893
1.00
13.51
B
C


ATOM
6972
CG
PHE
B
309
40.141
8.375
−24.170
1.00
13.46
B
C


ATOM
6973
CD1
PHE
B
309
40.722
9.533
−23.699
1.00
14.51
B
C


ATOM
6974
CE1
PHE
B
309
41.940
9.504
−23.011
1.00
16.19
B
C


ATOM
6975
CZ
PHE
B
309
42.567
8.281
−22.809
1.00
16.97
B
C


ATOM
6976
CE2
PHE
B
309
41.999
7.155
−23.238
1.00
15.71
B
C


ATOM
6977
CD2
PHE
B
309
40.772
7.180
−23.927
1.00
16.06
B
C


ATOM
6978
C
PHE
B
309
37.409
9.563
−23.164
1.00
14.01
B
C


ATOM
6979
O
PHE
B
309
37.782
9.622
−21.979
1.00
14.02
B
O


ATOM
6980
N
THR
B
310
36.886
10.610
−23.812
1.00
17.04
B
N


ATOM
6981
CA
THR
B
310
36.645
11.889
−23.157
1.00
15.23
B
C


ATOM
6982
CB
THR
B
310
36.062
12.936
−24.140
1.00
17.00
B
C


ATOM
6983
OG1
THR
B
310
36.923
13.003
−25.280
1.00
18.07
B
O


ATOM
6984
CG2
THR
B
310
35.942
14.349
−23.506
1.00
14.76
B
C


ATOM
6985
C
THR
B
310
35.729
11.735
−21.948
1.00
17.85
B
C


ATOM
6986
O
THR
B
310
36.039
12.243
−20.871
1.00
14.66
B
O


ATOM
6987
N
ASP
B
311
34.649
10.979
−22.130
1.00
19.67
B
N


ATOM
6988
CA
ASP
B
311
33.699
10.657
−21.075
1.00
19.68
B
C


ATOM
6989
CB
ASP
B
311
32.534
9.817
−21.647
1.00
22.14
B
C


ATOM
6990
CG
ASP
B
311
31.516
9.414
−20.579
1.00
28.93
B
C


ATOM
6991
OD1
ASP
B
311
30.837
10.310
−19.997
1.00
31.55
B
O


ATOM
6992
OD2
ASP
B
311
31.429
8.209
−20.261
1.00
32.04
B
O


ATOM
6993
C
ASP
B
311
34.372
9.886
−19.944
1.00
19.06
B
C


ATOM
6994
O
ASP
B
311
34.114
10.168
−18.785
1.00
16.44
B
O


ATOM
6995
N
ALA
B
312
35.229
8.914
−20.281
1.00
18.62
B
N


ATOM
6996
CA
ALA
B
312
35.926
8.147
−19.275
1.00
17.19
B
C


ATOM
6997
CB
ALA
B
312
36.664
6.912
−19.892
1.00
13.49
B
C


ATOM
6998
C
ALA
B
312
36.859
9.028
−18.440
1.00
13.94
B
C


ATOM
6999
O
ALA
B
312
36.894
8.886
−17.229
1.00
15.40
B
O


ATOM
7000
N
VAL
B
313
37.541
9.998
−19.063
1.00
13.78
B
N


ATOM
7001
CA
VAL
B
313
38.380
10.912
−18.337
1.00
14.72
B
C


ATOM
7002
CB
VAL
B
313
39.314
11.724
−19.271
1.00
12.81
B
C


ATOM
7003
CG1
VAL
B
313
40.012
12.823
−18.484
1.00
16.22
B
C


ATOM
7004
CG2
VAL
B
313
40.344
10.765
−19.930
1.00
12.97
B
C


ATOM
7005
C
VAL
B
313
37.556
11.845
−17.413
1.00
17.12
B
C


ATOM
7006
O
VAL
B
313
37.895
11.995
−16.248
1.00
13.85
B
O


ATOM
7007
N
GLU
B
314
36.446
12.384
−17.914
1.00
19.70
B
N


ATOM
7008
CA
GLU
B
314
35.519
13.203
−17.116
1.00
17.71
B
C


ATOM
7009
CB
GLU
B
314
34.314
13.642
−17.987
1.00
18.63
B
C


ATOM
7010
CG
GLU
B
314
34.732
14.588
−19.117
1.00
22.12
B
C


ATOM
7011
CD
GLU
B
314
33.579
15.065
−20.023
1.00
28.37
B
C


ATOM
7012
OE1
GLU
B
314
32.565
14.380
−20.150
1.00
33.47
B
O


ATOM
7013
OE2
GLU
B
314
33.738
16.100
−20.679
1.00
28.18
B
O


ATOM
7014
C
GLU
B
314
34.992
12.469
−15.881
1.00
20.83
B
C


ATOM
7015
O
GLU
B
314
34.964
13.042
−14.820
1.00
18.78
B
O


ATOM
7016
N
ARG
B
315
34.624
11.195
−16.017
1.00
17.42
B
N


ATOM
7017
CA
ARG
B
315
34.045
10.428
−14.888
1.00
21.50
B
C


ATOM
7018
CB
ARG
B
315
33.193
9.299
−15.421
1.00
24.86
B
C


ATOM
7019
CG
ARG
B
315
31.882
9.782
−16.058
1.00
31.82
B
C


ATOM
7020
CD
ARG
B
315
31.120
8.651
−16.749
1.00
38.64
B
C


ATOM
7021
NE
ARG
B
315
30.075
9.168
−17.641
1.00
56.06
B
N


ATOM
7022
CZ
ARG
B
315
28.896
9.671
−17.251
1.00
63.18
B
C


ATOM
7023
NH1
ARG
B
315
28.557
9.724
−15.966
1.00
58.00
B
N


ATOM
7024
NH2
ARG
B
315
28.034
10.116
−18.164
1.00
62.54
B
N


ATOM
7025
C
ARG
B
315
35.107
9.879
−13.924
1.00
20.36
B
C


ATOM
7026
O
ARG
B
315
34.860
9.690
−12.725
1.00
16.97
B
O


ATOM
7027
N
TRP
B
316
36.295
9.608
−14.445
1.00
15.84
B
N


ATOM
7028
CA
TRP
B
316
37.393
9.138
−13.622
1.00
14.10
B
C


ATOM
7029
CB
TRP
B
316
37.925
10.258
−12.740
1.00
15.69
B
C


ATOM
7030
CG
TRP
B
316
39.359
10.072
−12.373
1.00
12.64
B
C


ATOM
7031
CD1
TRP
B
316
39.871
9.691
−11.155
1.00
13.71
B
C


ATOM
7032
NE1
TRP
B
316
41.226
9.613
−11.213
1.00
11.47
B
N


ATOM
7033
CE2
TRP
B
316
41.677
9.923
−12.429
1.00
10.57
B
C


ATOM
7034
CD2
TRP
B
316
40.532
10.242
−13.240
1.00
11.76
B
C


ATOM
7035
CE3
TRP
B
316
40.720
10.599
−14.556
1.00
12.19
B
C


ATOM
7036
CZ3
TRP
B
316
41.996
10.637
−15.048
1.00
14.49
B
C


ATOM
7037
CH2
TRP
B
316
43.103
10.344
−14.234
1.00
14.28
B
C


ATOM
7038
CZ2
TRP
B
316
42.958
9.993
−12.914
1.00
12.17
B
C


ATOM
7039
C
TRP
B
316
36.997
7.951
−12.817
1.00
13.75
B
C


ATOM
7040
O
TRP
B
316
37.127
7.945
−11.613
1.00
15.54
B
O


ATOM
7041
N
ASP
B
317
36.470
6.939
−13.485
1.00
14.60
B
N


ATOM
7042
CA
ASP
B
317
35.944
5.750
−12.810
1.00
18.60
B
C


ATOM
7043
CB
ASP
B
317
34.439
5.688
−13.112
1.00
18.39
B
C


ATOM
7044
CG
ASP
B
317
33.744
4.490
−12.526
1.00
23.79
B
C


ATOM
7045
OD1
ASP
B
317
34.291
3.728
−11.691
1.00
28.29
B
O


ATOM
7046
OD2
ASP
B
317
32.578
4.326
−12.918
1.00
29.79
B
O


ATOM
7047
C
ASP
B
317
36.655
4.521
−13.365
1.00
17.48
B
C


ATOM
7048
O
ASP
B
317
36.458
4.195
−14.532
1.00
21.17
B
O


ATOM
7049
N
VAL
B
318
37.449
3.844
−12.535
1.00
20.98
B
N


ATOM
7050
CA
VAL
B
318
38.216
2.676
−12.970
1.00
18.72
B
C


ATOM
7051
CB
VAL
B
318
39.303
2.293
−11.952
1.00
22.93
B
C


ATOM
7052
CG1
VAL
B
318
38.693
1.638
−10.707
1.00
22.40
B
C


ATOM
7053
CG2
VAL
B
318
40.351
1.365
−12.578
1.00
21.60
B
C


ATOM
7054
C
VAL
B
318
37.276
1.489
−13.145
1.00
26.77
B
C


ATOM
7055
O
VAL
B
318
37.547
0.597
−13.934
1.00
24.06
B
O


ATOM
7056
N
ASN
B
319
36.167
1.476
−12.407
1.00
24.55
B
N


ATOM
7057
CA
ASN
B
319
35.197
0.365
−12.484
1.00
25.94
B
C


ATOM
7058
CB
ASN
B
319
34.261
0.385
−11.275
1.00
26.17
B
C


ATOM
7059
CG
ASN
B
319
35.018
0.199
−9.952
1.00
28.06
B
C


ATOM
7060
OD1
ASN
B
319
35.796
−0.761
−9.803
1.00
27.13
B
O


ATOM
7061
ND2
ASN
B
319
34.789
1.100
−8.988
1.00
20.00
B
N


ATOM
7062
C
ASN
B
319
34.393
0.345
−13.769
1.00
23.58
B
C


ATOM
7063
O
ASN
B
319
33.598
−0.555
−13.949
1.00
23.76
B
O


ATOM
7064
N
ALA
B
320
34.636
1.308
−14.663
1.00
23.98
B
N


ATOM
7065
CA
ALA
B
320
33.974
1.397
−15.984
1.00
29.16
B
C


ATOM
7066
CB
ALA
B
320
33.189
2.730
−16.067
1.00
26.97
B
C


ATOM
7067
C
ALA
B
320
34.936
1.331
−17.199
1.00
33.61
B
C


ATOM
7068
O
ALA
B
320
34.527
1.638
−18.303
1.00
29.23
B
O


ATOM
7069
N
ILE
B
321
36.209
0.965
−17.021
1.00
33.27
B
N


ATOM
7070
CA
ILE
B
321
37.157
0.884
−18.166
1.00
22.33
B
C


ATOM
7071
CB
ILE
B
321
38.641
0.637
−17.725
1.00
25.50
B
C


ATOM
7072
CG1
ILE
B
321
38.835
−0.765
−17.202
1.00
23.16
B
C


ATOM
7073
CD1
ILE
B
321
40.201
−0.992
−16.573
1.00
33.57
B
C


ATOM
7074
CG2
ILE
B
321
39.096
1.664
−16.636
1.00
22.17
B
C


ATOM
7075
C
ILE
B
321
36.784
−0.196
−19.191
1.00
29.18
B
C


ATOM
7076
O
ILE
B
321
37.217
−0.143
−20.339
1.00
33.50
B
O


ATOM
7077
N
ASN
B
322
35.992
−1.186
−18.789
1.00
26.39
B
N


ATOM
7078
CA
ASN
B
322
35.492
−2.174
−19.744
1.00
28.36
B
C


ATOM
7079
CB
ASN
B
322
34.846
−3.373
−19.019
1.00
29.64
B
C


ATOM
7080
CG
ASN
B
322
35.896
−4.329
−18.370
1.00
28.34
B
C


ATOM
7081
OD1
ASN
B
322
37.100
−4.289
−18.666
1.00
22.33
B
O


ATOM
7082
ND2
ASN
B
322
35.421
−5.182
−17.500
1.00
23.59
B
N


ATOM
7083
C
ASN
B
322
34.547
−1.591
−20.842
1.00
24.07
B
C


ATOM
7084
O
ASN
B
322
34.299
−2.245
−21.813
1.00
22.82
B
O


ATOM
7085
N
ASP
B
323
34.086
−0.358
−20.713
1.00
26.94
B
N


ATOM
7086
CA
ASP
B
323
33.367
0.317
−21.834
1.00
34.72
B
C


ATOM
7087
CB
ASP
B
323
32.720
1.634
−21.343
1.00
42.84
B
C


ATOM
7088
CG
ASP
B
323
31.791
1.459
−20.127
1.00
42.61
B
C


ATOM
7089
OD1
ASP
B
323
31.199
0.384
−19.955
1.00
44.74
B
O


ATOM
7090
OD2
ASP
B
323
31.641
2.433
−19.353
1.00
43.15
B
O


ATOM
7091
C
ASP
B
323
34.292
0.705
−23.032
1.00
27.41
B
C


ATOM
7092
O
ASP
B
323
33.835
1.019
−24.125
1.00
27.02
B
O


ATOM
7093
N
LEU
B
324
35.594
0.723
−22.806
1.00
21.91
B
N


ATOM
7094
CA
LEU
B
324
36.520
1.263
−23.795
1.00
21.35
B
C


ATOM
7095
CB
LEU
B
324
37.699
1.905
−23.075
1.00
18.13
B
C


ATOM
7096
CG
LEU
B
324
37.394
3.110
−22.196
1.00
19.17
B
C


ATOM
7097
CD1
LEU
B
324
38.555
3.482
−21.265
1.00
17.09
B
C


ATOM
7098
CD2
LEU
B
324
37.033
4.301
−23.103
1.00
15.35
B
C


ATOM
7099
C
LEU
B
324
37.058
0.190
−24.738
1.00
20.92
B
C


ATOM
7100
O
LEU
B
324
37.287
−0.944
−24.307
1.00
19.02
B
O


ATOM
7101
N
PRO
B
325
37.350
0.556
−26.009
1.00
18.20
B
N


ATOM
7102
CA
PRO
B
325
38.042
−0.408
−26.878
1.00
21.89
B
C


ATOM
7103
CB
PRO
B
325
38.135
0.326
−28.230
1.00
21.00
B
C


ATOM
7104
CG
PRO
B
325
38.016
1.718
−27.914
1.00
20.12
B
C


ATOM
7105
CD
PRO
B
325
37.143
1.842
−26.684
1.00
18.81
B
C


ATOM
7106
C
PRO
B
325
39.426
−0.741
−26.304
1.00
19.63
B
C


ATOM
7107
O
PRO
B
325
39.976
0.057
−25.533
1.00
15.57
B
O


ATOM
7108
N
ASP
B
326
39.964
−1.908
−26.656
1.00
16.21
B
N


ATOM
7109
CA
ASP
B
326
41.121
−2.488
−25.940
1.00
17.77
B
C


ATOM
7110
CB
ASP
B
326
41.543
−3.815
−26.570
1.00
22.34
B
C


ATOM
7111
CG
ASP
B
326
40.567
−4.979
−26.255
1.00
24.51
B
C


ATOM
7112
OD1
ASP
B
326
39.698
−4.857
−25.367
1.00
28.55
B
O


ATOM
7113
OD2
ASP
B
326
40.673
−6.028
−26.910
1.00
29.72
B
O


ATOM
7114
C
ASP
B
326
42.322
−1.526
−25.867
1.00
16.53
B
C


ATOM
7115
O
ASP
B
326
42.909
−1.335
−24.792
1.00
13.99
B
O


ATOM
7116
N
TYR
B
327
42.654
−0.857
−26.972
1.00
15.60
B
N


ATOM
7117
CA
TYR
B
327
43.804
0.098
−26.924
1.00
17.04
B
C


ATOM
7118
CB
TYR
B
327
44.214
0.584
−28.316
1.00
17.03
B
C


ATOM
7119
CG
TYR
B
327
43.315
1.659
−28.914
1.00
17.73
B
C


ATOM
7120
CD1
TYR
B
327
42.132
1.343
−29.557
1.00
18.37
B
C


ATOM
7121
CE1
TYR
B
327
41.316
2.363
−30.095
1.00
19.68
B
C


ATOM
7122
CZ
TYR
B
327
41.741
3.685
−30.012
1.00
19.30
B
C


ATOM
7123
OH
TYR
B
327
41.008
4.703
−30.533
1.00
17.39
B
O


ATOM
7124
CE2
TYR
B
327
42.902
3.998
−29.399
1.00
21.08
B
C


ATOM
7125
CD2
TYR
B
327
43.684
2.991
−28.842
1.00
19.32
B
C


ATOM
7126
C
TYR
B
327
43.563
1.274
−25.978
1.00
15.40
B
C


ATOM
7127
O
TYR
B
327
44.494
1.753
−25.289
1.00
17.13
B
O


ATOM
7128
N
MET
B
328
42.326
1.737
−25.879
1.00
15.12
B
N


ATOM
7129
CA
MET
B
328
42.043
2.839
−24.944
1.00
13.87
B
C


ATOM
7130
CB
MET
B
328
40.746
3.533
−25.291
1.00
16.27
B
C


ATOM
7131
CG
MET
B
328
40.878
4.478
−26.468
1.00
17.30
B
C


ATOM
7132
SD
MET
B
328
39.258
5.116
−26.958
1.00
16.70
B
S


ATOM
7133
CE
MET
B
328
39.865
6.551
−27.939
1.00
15.53
B
C


ATOM
7134
C
MET
B
328
41.994
2.405
−23.485
1.00
13.82
B
C


ATOM
7135
O
MET
B
328
42.280
3.190
−22.595
1.00
15.80
B
O


ATOM
7136
N
LYS
B
329
41.647
1.157
−23.258
1.00
15.99
B
N


ATOM
7137
CA
LYS
B
329
41.602
0.607
−21.905
1.00
18.78
B
C


ATOM
7138
CB
LYS
B
329
41.141
−0.836
−21.963
1.00
18.76
B
C


ATOM
7139
CG
LYS
B
329
40.485
−1.341
−20.692
1.00
29.84
B
C


ATOM
7140
CD
LYS
B
329
39.870
−2.750
−20.875
1.00
29.88
B
C


ATOM
7141
CE
LYS
B
329
39.085
−2.869
−22.175
1.00
34.22
B
C


ATOM
7142
NZ
LYS
B
329
38.141
−4.004
−22.166
1.00
33.90
B
N


ATOM
7143
C
LYS
B
329
42.982
0.688
−21.275
1.00
15.84
B
C


ATOM
7144
O
LYS
B
329
43.124
1.102
−20.145
1.00
13.96
B
O


ATOM
7145
N
LEU
B
330
43.988
0.286
−22.040
1.00
13.82
B
N


ATOM
7146
CA
LEU
B
330
45.348
0.326
−21.593
1.00
14.18
B
C


ATOM
7147
CB
LEU
B
330
46.220
−0.428
−22.591
1.00
14.87
B
C


ATOM
7148
CG
LEU
B
330
47.602
−0.861
−22.123
1.00
17.60
B
C


ATOM
7149
CD1
LEU
B
330
47.498
−1.976
−21.081
1.00
17.42
B
C


ATOM
7150
CD2
LEU
B
330
48.411
−1.377
−23.338
1.00
19.62
B
C


ATOM
7151
C
LEU
B
330
45.839
1.739
−21.374
1.00
15.98
B
C


ATOM
7152
O
LEU
B
330
46.381
2.064
−20.296
1.00
14.92
B
O


ATOM
7153
N
CYS
B
331
45.638
2.607
−22.372
1.00
15.80
B
N


ATOM
7154
CA
ACYS
B
331
46.049
4.000
−22.251
0.50
14.90
B
C


ATOM
7155
CA
BCYS
B
331
46.018
4.013
−22.258
0.50
16.77
B
C


ATOM
7156
CB
ACYS
B
331
45.751
4.763
−23.555
0.50
16.03
B
C


ATOM
7157
CB
BCYS
B
331
45.609
4.772
−23.525
0.50
21.14
B
C


ATOM
7158
SG
ACYS
B
331
46.485
6.421
−23.637
0.50
17.14
B
S


ATOM
7159
SG
BCYS
B
331
46.673
4.456
−24.908
0.50
30.01
B
S


ATOM
7160
C
CYS
B
331
45.360
4.680
−21.061
1.00
14.16
B
C


ATOM
7161
O
CYS
B
331
46.017
5.344
−20.269
1.00
14.78
B
O


ATOM
7162
N
PHE
B
332
44.040
4.517
−20.956
1.00
12.94
B
N


ATOM
7163
CA
PHE
B
332
43.289
5.070
−19.843
1.00
12.85
B
C


ATOM
7164
CB
PHE
B
332
41.792
4.785
−19.934
1.00
14.46
B
C


ATOM
7165
CG
PHE
B
332
40.993
5.396
−18.801
1.00
17.76
B
C


ATOM
7166
CD1
PHE
B
332
40.638
6.716
−18.836
1.00
17.42
B
C


ATOM
7167
CE1
PHE
B
332
39.921
7.282
−17.798
1.00
21.26
B
C


ATOM
7168
CZ
PHE
B
332
39.554
6.517
−16.719
1.00
17.35
B
C


ATOM
7169
CE2
PHE
B
332
39.930
5.236
−16.639
1.00
20.18
B
C


ATOM
7170
CD2
PHE
B
332
40.641
4.650
−17.689
1.00
21.54
B
C


ATOM
7171
C
PHE
B
332
43.816
4.591
−18.496
1.00
10.49
B
C


ATOM
7172
O
PHE
B
332
44.073
5.398
−17.613
1.00
11.44
B
O


ATOM
7173
N
LEU
B
333
44.002
3.293
−18.351
1.00
11.57
B
N


ATOM
7174
CA
LEU
B
333
44.347
2.762
−17.044
1.00
10.73
B
C


ATOM
7175
CB
LEU
B
333
44.170
1.256
−16.957
1.00
11.15
B
C


ATOM
7176
CG
LEU
B
333
44.494
0.599
−15.595
1.00
11.32
B
C


ATOM
7177
CD1
LEU
B
333
43.650
1.232
−14.483
1.00
9.46
B
C


ATOM
7178
CD2
LEU
B
333
44.261
−0.957
−15.619
1.00
9.98
B
C


ATOM
7179
C
LEU
B
333
45.771
3.231
−16.673
1.00
13.03
B
C


ATOM
7180
O
LEU
B
333
46.024
3.606
−15.507
1.00
10.42
B
O


ATOM
7181
N
ALA
B
334
46.664
3.338
−17.663
1.00
11.93
B
N


ATOM
7182
CA
ALA
B
334
48.011
3.853
−17.368
1.00
13.38
B
C


ATOM
7183
CB
ALA
B
334
48.913
3.685
−18.575
1.00
15.36
B
C


ATOM
7184
C
ALA
B
334
47.984
5.312
−16.906
1.00
12.41
B
C


ATOM
7185
O
ALA
B
334
48.691
5.696
−15.978
1.00
12.48
B
O


ATOM
7186
N
LEU
B
335
47.156
6.123
−17.548
1.00
14.27
B
N


ATOM
7187
CA
LEU
B
335
47.000
7.544
−17.184
1.00
14.27
B
C


ATOM
7188
CB
LEU
B
335
46.099
8.266
−18.217
1.00
13.34
B
C


ATOM
7189
CG
LEU
B
335
45.755
9.740
−17.994
1.00
19.00
B
C


ATOM
7190
CD1
LEU
B
335
47.010
10.582
−18.057
1.00
16.63
B
C


ATOM
7191
CD2
LEU
B
335
44.695
10.245
−18.956
1.00
21.42
B
C


ATOM
7192
C
LEU
B
335
46.396
7.663
−15.779
1.00
13.65
B
C


ATOM
7193
O
LEU
B
335
46.898
8.412
−14.942
1.00
15.12
B
O


ATOM
7194
N
TYR
B
336
45.309
6.926
−15.574
1.00
14.28
B
N


ATOM
7195
CA
TYR
B
336
44.563
6.865
−14.329
1.00
13.79
B
C


ATOM
7196
CB
TYR
B
336
43.503
5.777
−14.448
1.00
13.43
B
C


ATOM
7197
CG
TYR
B
336
42.499
5.696
−13.337
1.00
12.12
B
C


ATOM
7198
CD1
TYR
B
336
41.410
6.570
−13.286
1.00
14.48
B
C


ATOM
7199
CE1
TYR
B
336
40.461
6.486
−12.232
1.00
15.49
B
C


ATOM
7200
CZ
TYR
B
336
40.618
5.543
−11.262
1.00
17.58
B
C


ATOM
7201
OH
TYR
B
336
39.706
5.404
−10.223
1.00
20.26
B
O


ATOM
7202
CE2
TYR
B
336
41.705
4.678
−11.313
1.00
16.06
B
C


ATOM
7203
CD2
TYR
B
336
42.636
4.775
−12.328
1.00
12.06
B
C


ATOM
7204
C
TYR
B
336
45.474
6.574
−13.173
1.00
13.04
B
C


ATOM
7205
O
TYR
B
336
45.535
7.368
−12.217
1.00
10.25
B
O


ATOM
7206
N
ASN
B
337
46.268
5.507
−13.279
1.00
11.68
B
N


ATOM
7207
CA
ASN
B
337
47.194
5.162
−12.199
1.00
11.43
B
C


ATOM
7208
CB
ASN
B
337
47.852
3.796
−12.409
1.00
10.75
B
C


ATOM
7209
CG
ASN
B
337
46.888
2.659
−12.265
1.00
13.68
B
C


ATOM
7210
OD1
ASN
B
337
45.820
2.821
−11.681
1.00
15.73
B
O


ATOM
7211
ND2
ASN
B
337
47.246
1.475
−12.815
1.00
12.01
B
N


ATOM
7212
C
ASN
B
337
48.268
6.230
−12.014
1.00
12.56
B
C


ATOM
7213
O
ASN
B
337
48.669
6.532
−10.907
1.00
9.49
B
O


ATOM
7214
N
THR
B
338
48.762
6.796
−13.114
1.00
11.89
B
N


ATOM
7215
CA
THR
B
338
49.845
7.794
−13.007
1.00
13.27
B
C


ATOM
7216
CB
THR
B
338
50.380
8.190
−14.415
1.00
13.32
B
C


ATOM
7217
OG1
THR
B
338
50.891
7.025
−15.072
1.00
11.43
B
O


ATOM
7218
CG2
THR
B
338
51.537
9.254
−14.292
1.00
11.47
B
C


ATOM
7219
C
THR
B
338
49.354
9.039
−12.245
1.00
11.70
B
C


ATOM
7220
O
THR
B
338
50.025
9.546
−11.331
1.00
10.36
B
O


ATOM
7221
N
ILE
B
339
48.151
9.488
−12.577
1.00
13.01
B
N


ATOM
7222
CA
ILE
B
339
47.617
10.696
−11.960
1.00
14.00
B
C


ATOM
7223
CB
ILE
B
339
46.396
11.246
−12.721
1.00
14.99
B
C


ATOM
7224
CG1
ILE
B
339
46.747
11.682
−14.146
1.00
18.07
B
C


ATOM
7225
CD1
ILE
B
339
48.106
12.170
−14.359
1.00
20.10
B
C


ATOM
7226
CG2
ILE
B
339
45.768
12.404
−11.959
1.00
14.32
B
C


ATOM
7227
C
ILE
B
339
47.242
10.398
−10.485
1.00
13.46
B
C


ATOM
7228
O
ILE
B
339
47.538
11.205
−9.621
1.00
11.31
B
O


ATOM
7229
N
ASN
B
340
46.644
9.230
−10.215
1.00
13.92
B
N


ATOM
7230
CA
ASN
B
340
46.307
8.829
−8.836
1.00
13.55
B
C


ATOM
7231
CB
ASN
B
340
45.468
7.553
−8.764
1.00
12.74
B
C


ATOM
7232
CG
ASN
B
340
44.074
7.755
−9.301
1.00
15.12
B
C


ATOM
7233
OD1
ASN
B
340
43.648
8.889
−9.555
1.00
13.91
B
O


ATOM
7234
ND2
ASN
B
340
43.364
6.666
−9.520
1.00
12.09
B
N


ATOM
7235
C
ASN
B
340
47.539
8.718
−7.965
1.00
14.94
B
C


ATOM
7236
O
ASN
B
340
47.471
9.006
−6.759
1.00
12.07
B
O


ATOM
7237
N
GLU
B
341
48.656
8.300
−8.559
1.00
13.96
B
N


ATOM
7238
CA
GLU
B
341
49.949
8.274
−7.847
1.00
12.04
B
C


ATOM
7239
CB
GLU
B
341
50.982
7.440
−8.654
1.00
16.76
B
C


ATOM
7240
CG
GLU
B
341
52.351
7.360
−8.012
1.00
27.37
B
C


ATOM
7241
CD
GLU
B
341
53.300
6.365
−8.704
1.00
40.42
B
C


ATOM
7242
OE1
GLU
B
341
53.521
6.477
−9.939
1.00
50.64
B
O


ATOM
7243
OE2
GLU
B
341
53.825
5.477
−8.000
1.00
41.15
B
O


ATOM
7244
C
GLU
B
341
50.483
9.651
−7.494
1.00
11.88
B
C


ATOM
7245
O
GLU
B
341
50.999
9.868
−6.379
1.00
11.32
B
O


ATOM
7246
N
ILE
B
342
50.377
10.589
−8.421
1.00
11.62
B
N


ATOM
7247
CA
ILE
B
342
50.815
11.964
−8.141
1.00
13.66
B
C


ATOM
7248
CB
ILE
B
342
50.798
12.812
−9.390
1.00
14.05
B
C


ATOM
7249
CG1
ILE
B
342
51.936
12.339
−10.318
1.00
14.25
B
C


ATOM
7250
CD1
ILE
B
342
51.739
12.813
−11.820
1.00
13.16
B
C


ATOM
7251
CG2
ILE
B
342
50.943
14.326
−9.035
1.00
13.52
B
C


ATOM
7252
C
ILE
B
342
49.927
12.546
−7.060
1.00
12.69
B
C


ATOM
7253
O
ILE
B
342
50.446
13.164
−6.143
1.00
12.41
B
O


ATOM
7254
N
ALA
B
343
48.612
12.272
−7.119
1.00
13.09
B
N


ATOM
7255
CA
ALA
B
343
47.690
12.675
−6.016
1.00
13.78
B
C


ATOM
7256
CB
ALA
B
343
46.251
12.353
−6.322
1.00
10.48
B
C


ATOM
7257
C
ALA
B
343
48.088
12.082
−4.680
1.00
14.93
B
C


ATOM
7258
O
ALA
B
343
48.080
12.776
−3.639
1.00
12.57
B
O


ATOM
7259
N
TYR
B
344
48.450
10.802
−4.685
1.00
13.63
B
N


ATOM
7260
CA
TYR
B
344
48.910
10.153
−3.477
1.00
10.64
B
C


ATOM
7261
CB
TYR
B
344
49.202
8.632
−3.662
1.00
9.56
B
C


ATOM
7262
CG
TYR
B
344
49.722
8.019
−2.401
1.00
8.14
B
C


ATOM
7263
CD1
TYR
B
344
48.845
7.584
−1.425
1.00
8.38
B
C


ATOM
7264
CE1
TYR
B
344
49.276
7.038
−0.280
1.00
7.58
B
C


ATOM
7265
CZ
TYR
B
344
50.627
6.927
−0.020
1.00
8.20
B
C


ATOM
7266
OH
TYR
B
344
50.992
6.367
1.189
1.00
8.47
B
O


ATOM
7267
CE2
TYR
B
344
51.540
7.361
−0.959
1.00
8.57
B
C


ATOM
7268
CD2
TYR
B
344
51.078
7.891
−2.157
1.00
7.92
B
C


ATOM
7269
C
TYR
B
344
50.148
10.855
−2.903
1.00
11.53
B
C


ATOM
7270
O
TYR
B
344
50.207
11.110
−1.717
1.00
8.74
B
O


ATOM
7271
N
ASP
B
345
51.118
11.169
−3.744
1.00
12.70
B
N


ATOM
7272
CA
ASP
B
345
52.347
11.824
−3.292
1.00
14.02
B
C


ATOM
7273
CB
ASP
B
345
53.313
12.105
−4.464
1.00
18.53
B
C


ATOM
7274
CG
ASP
B
345
53.945
10.831
−5.042
1.00
27.30
B
C


ATOM
7275
OD1
ASP
B
345
53.971
9.815
−4.337
1.00
28.65
B
O


ATOM
7276
OD2
ASP
B
345
54.395
10.863
−6.211
1.00
40.96
B
O


ATOM
7277
C
ASP
B
345
52.011
13.157
−2.607
1.00
15.72
B
C


ATOM
7278
O
ASP
B
345
52.598
13.497
−1.579
1.00
13.12
B
O


ATOM
7279
N
ASN
B
346
51.055
13.878
−3.173
1.00
16.72
B
N


ATOM
7280
CA
ASN
B
346
50.683
15.159
−2.628
1.00
16.11
B
C


ATOM
7281
CB
ASN
B
346
49.923
15.942
−3.654
1.00
17.94
B
C


ATOM
7282
CG
ASN
B
346
50.806
16.715
−4.511
1.00
31.41
B
C


ATOM
7283
OD1
ASN
B
346
51.179
17.863
−4.162
1.00
25.07
B
O


ATOM
7284
ND2
ASN
B
346
51.187
16.121
−5.669
1.00
29.47
B
N


ATOM
7285
C
ASN
B
346
49.870
15.054
−1.342
1.00
16.09
B
C


ATOM
7286
O
ASN
B
346
50.053
15.856
−0.437
1.00
16.82
B
O


ATOM
7287
N
LEU
B
347
49.016
14.043
−1.237
1.00
14.19
B
N


ATOM
7288
CA
LEU
B
347
48.279
13.810
−0.010
1.00
15.35
B
C


ATOM
7289
CB
LEU
B
347
47.269
12.671
−0.191
1.00
13.99
B
C


ATOM
7290
CG
LEU
B
347
46.433
12.274
1.017
1.00
14.99
B
C


ATOM
7291
CD1
LEU
B
347
45.598
13.471
1.531
1.00
15.83
B
C


ATOM
7292
CD2
LEU
B
347
45.561
11.037
0.756
1.00
14.36
B
C


ATOM
7293
C
LEU
B
347
49.271
13.518
1.099
1.00
17.90
B
C


ATOM
7294
O
LEU
B
347
49.217
14.116
2.208
1.00
14.50
B
O


ATOM
7295
N
LYS
B
348
50.214
12.636
0.795
1.00
15.21
B
N


ATOM
7296
CA
LYS
B
348
51.180
12.206
1.787
1.00
19.60
B
C


ATOM
7297
CB
LYS
B
348
52.069
11.083
1.225
1.00
20.15
B
C


ATOM
7298
CG
LYS
B
348
53.053
10.521
2.188
1.00
23.64
B
C


ATOM
7299
CD
LYS
B
348
53.725
9.259
1.636
1.00
28.85
B
C


ATOM
7300
CE
LYS
B
348
55.190
9.441
1.319
1.00
36.11
B
C


ATOM
7301
NZ
LYS
B
348
55.383
10.058
0.015
1.00
38.70
B
N


ATOM
7302
C
LYS
B
348
52.051
13.350
2.244
1.00
16.80
B
C


ATOM
7303
O
LYS
B
348
52.398
13.439
3.429
1.00
16.67
B
O


ATOM
7304
N
ASP
B
349
52.484
14.170
1.291
1.00
18.71
B
N


ATOM
7305
CA
ASP
B
349
53.558
15.121
1.555
1.00
20.44
B
C


ATOM
7306
CB
ASP
B
349
54.430
15.332
0.304
1.00
23.50
B
C


ATOM
7307
CG
ASP
B
349
55.315
14.111
−0.042
1.00
32.24
B
C


ATOM
7308
OD1
ASP
B
349
55.586
13.246
0.842
1.00
32.69
B
O


ATOM
7309
OD2
ASP
B
349
55.722
14.020
−1.223
1.00
43.22
B
O


ATOM
7310
C
ASP
B
349
53.005
16.479
2.011
1.00
23.89
B
C


ATOM
7311
O
ASP
B
349
53.632
17.161
2.793
1.00
22.40
B
O


ATOM
7312
N
LYS
B
350
51.842
16.861
1.511
1.00
21.29
B
N


ATOM
7313
CA
LYS
B
350
51.274
18.175
1.791
1.00
22.34
B
C


ATOM
7314
CB
LYS
B
350
51.010
18.888
0.479
1.00
27.25
B
C


ATOM
7315
CG
LYS
B
350
52.261
19.230
−0.327
1.00
29.83
B
C


ATOM
7316
CD
LYS
B
350
51.788
19.978
−1.562
1.00
45.65
B
C


ATOM
7317
CE
LYS
B
350
52.786
20.029
−2.703
1.00
44.16
B
C


ATOM
7318
NZ
LYS
B
350
52.019
20.581
−3.865
1.00
42.33
B
N


ATOM
7319
C
LYS
B
350
49.964
18.091
2.567
1.00
26.95
B
C


ATOM
7320
O
LYS
B
350
49.430
19.108
2.998
1.00
20.13
B
O


ATOM
7321
N
GLY
B
351
49.427
16.890
2.735
1.00
18.56
B
N


ATOM
7322
CA
GLY
B
351
48.193
16.717
3.487
1.00
19.14
B
C


ATOM
7323
C
GLY
B
351
46.946
17.172
2.759
1.00
18.66
B
C


ATOM
7324
O
GLY
B
351
45.922
17.388
3.386
1.00
21.25
B
O


ATOM
7325
N
GLU
B
352
47.017
17.307
1.438
1.00
17.28
B
N


ATOM
7326
CA
GLU
B
352
45.875
17.795
0.649
1.00
20.07
B
C


ATOM
7327
CB
GLU
B
352
46.277
19.103
−0.050
1.00
25.44
B
C


ATOM
7328
CG
GLU
B
352
46.764
20.227
0.882
1.00
34.41
B
C


ATOM
7329
CD
GLU
B
352
45.648
21.027
1.532
1.00
40.95
B
C


ATOM
7330
OE1
GLU
B
352
44.479
20.596
1.468
1.00
43.14
B
O


ATOM
7331
OE2
GLU
B
352
45.957
22.097
2.119
1.00
53.99
B
O


ATOM
7332
C
GLU
B
352
45.491
16.839
−0.452
1.00
17.35
B
C


ATOM
7333
O
GLU
B
352
46.371
16.306
−1.119
1.00
15.99
B
O


ATOM
7334
N
ASN
B
353
44.198
16.692
−0.693
1.00
20.08
B
N


ATOM
7335
CA
ASN
B
353
43.687
15.949
−1.826
1.00
24.17
B
C


ATOM
7336
CB
ASN
B
353
42.344
15.291
−1.505
1.00
24.41
B
C


ATOM
7337
CG
ASN
B
353
41.823
14.425
−2.660
1.00
28.25
B
C


ATOM
7338
OD1
ASN
B
353
42.443
14.351
−3.726
1.00
19.89
B
O


ATOM
7339
ND2
ASN
B
353
40.711
13.719
−2.426
1.00
28.61
B
N


ATOM
7340
C
ASN
B
353
43.523
16.860
−3.035
1.00
24.82
B
C


ATOM
7341
O
ASN
B
353
42.539
17.565
−3.142
1.00
20.76
B
O


ATOM
7342
N
ILE
B
354
44.464
16.788
−3.962
1.00
17.07
B
N


ATOM
7343
CA
ILE
B
354
44.447
17.612
−5.161
1.00
16.52
B
C


ATOM
7344
CB
ILE
B
354
45.875
18.138
−5.458
1.00
19.20
B
C


ATOM
7345
CG1
ILE
B
354
46.813
16.975
−5.801
1.00
20.65
B
C


ATOM
7346
CD1
ILE
B
354
48.124
17.359
−6.405
1.00
19.75
B
C


ATOM
7347
CG2
ILE
B
354
46.394
18.954
−4.308
1.00
20.17
B
C


ATOM
7348
C
ILE
B
354
43.878
16.873
−6.406
1.00
14.87
B
C


ATOM
7349
O
ILE
B
354
43.858
17.419
−7.510
1.00
14.28
B
O


ATOM
7350
N
LEU
B
355
43.395
15.655
−6.233
1.00
14.49
B
N


ATOM
7351
CA
LEU
B
355
42.992
14.814
−7.380
1.00
16.72
B
C


ATOM
7352
CB
LEU
B
355
42.503
13.437
−6.905
1.00
16.17
B
C


ATOM
7353
CG
LEU
B
355
42.124
12.443
−8.018
1.00
20.13
B
C


ATOM
7354
CD1
LEU
B
355
43.327
12.200
−8.933
1.00
16.30
B
C


ATOM
7355
CD2
LEU
B
355
41.576
11.152
−7.429
1.00
19.09
B
C


ATOM
7356
C
LEU
B
355
41.932
15.525
−8.255
1.00
17.42
B
C


ATOM
7357
O
LEU
B
355
42.073
15.554
−9.498
1.00
17.72
B
O


ATOM
7358
N
PRO
B
356
40.919
16.176
−7.616
1.00
19.20
B
N


ATOM
7359
CA
PRO
B
356
39.907
16.857
−8.447
1.00
20.49
B
C


ATOM
7360
CB
PRO
B
356
39.011
17.570
−7.427
1.00
25.18
B
C


ATOM
7361
CG
PRO
B
356
39.195
16.766
−6.140
1.00
22.46
B
C


ATOM
7362
CD
PRO
B
356
40.646
16.349
−6.176
1.00
16.95
B
C


ATOM
7363
C
PRO
B
356
40.531
17.846
−9.417
1.00
16.26
B
C


ATOM
7364
O
PRO
B
356
40.087
17.924
−10.549
1.00
15.34
B
O


ATOM
7365
N
TYR
B
357
41.588
18.532
−9.021
1.00
15.15
B
N


ATOM
7366
CA
TYR
B
357
42.214
19.540
−9.920
1.00
18.77
B
C


ATOM
7367
CB
TYR
B
357
43.092
20.465
−9.121
1.00
24.64
B
C


ATOM
7368
CG
TYR
B
357
42.401
20.969
−7.856
1.00
25.80
B
C


ATOM
7369
CD1
TYR
B
357
41.238
21.715
−7.918
1.00
35.57
B
C


ATOM
7370
CE1
TYR
B
357
40.594
22.159
−6.749
1.00
34.51
B
C


ATOM
7371
CZ
TYR
B
357
41.141
21.848
−5.528
1.00
34.02
B
C


ATOM
7372
OH
TYR
B
357
40.570
22.254
−4.355
1.00
40.33
B
O


ATOM
7373
CE2
TYR
B
357
42.290
21.112
−5.458
1.00
30.39
B
C


ATOM
7374
CD2
TYR
B
357
42.914
20.684
−6.621
1.00
25.62
B
C


ATOM
7375
C
TYR
B
357
43.028
18.907
−11.061
1.00
19.51
B
C


ATOM
7376
O
TYR
B
357
43.054
19.415
−12.191
1.00
14.78
B
O


ATOM
7377
N
LEU
B
358
43.688
17.793
−10.758
1.00
16.40
B
N


ATOM
7378
CA
LEU
B
358
44.472
17.065
−11.759
1.00
14.91
B
C


ATOM
7379
CB
LEU
B
358
45.363
16.019
−11.095
1.00
16.54
B
C


ATOM
7380
CG
LEU
B
358
46.329
16.457
−9.988
1.00
18.96
B
C


ATOM
7381
CD1
LEU
B
358
47.129
15.226
−9.464
1.00
15.05
B
C


ATOM
7382
CD2
LEU
B
358
47.237
17.613
−10.471
1.00
17.94
B
C


ATOM
7383
C
LEU
B
358
43.563
16.413
−12.781
1.00
12.49
B
C


ATOM
7384
O
LEU
B
358
43.850
16.409
−13.985
1.00
14.97
B
O


ATOM
7385
N
THR
B
359
42.470
15.823
−12.327
1.00
14.36
B
N


ATOM
7386
CA
THR
B
359
41.552
15.178
−13.273
1.00
15.09
B
C


ATOM
7387
CB
THR
B
359
40.646
14.193
−12.575
1.00
17.38
B
C


ATOM
7388
OG1
THR
B
359
39.798
14.921
−11.679
1.00
20.37
B
O


ATOM
7389
CG2
THR
B
359
41.516
13.140
−11.799
1.00
13.68
B
C


ATOM
7390
C
THR
B
359
40.754
16.185
−14.129
1.00
16.20
B
C


ATOM
7391
O
THR
B
359
40.454
15.917
−15.302
1.00
17.64
B
O


ATOM
7392
N
LYS
B
360
40.458
17.360
−13.583
1.00
16.58
B
N


ATOM
7393
CA
LYS
B
360
39.785
18.406
−14.375
1.00
15.55
B
C


ATOM
7394
CB
LYS
B
360
39.361
19.567
−13.475
1.00
20.66
B
C


ATOM
7395
CG
LYS
B
360
38.529
20.692
−14.207
1.00
25.93
B
C


ATOM
7396
CD
LYS
B
360
37.361
20.084
−14.960
1.00
33.77
B
C


ATOM
7397
CE
LYS
B
360
36.242
21.101
−15.274
1.00
45.52
B
C


ATOM
7398
NZ
LYS
B
360
36.763
22.220
−16.100
1.00
38.16
B
N


ATOM
7399
C
LYS
B
360
40.699
18.905
−15.461
1.00
14.49
B
C


ATOM
7400
O
LYS
B
360
40.254
19.149
−16.573
1.00
17.23
B
O


ATOM
7401
N
ALA
B
361
41.979
19.061
−15.161
1.00
13.46
B
N


ATOM
7402
CA
ALA
B
361
42.921
19.464
−16.193
1.00
13.85
B
C


ATOM
7403
CB
ALA
B
361
44.341
19.705
−15.653
1.00
12.78
B
C


ATOM
7404
C
ALA
B
361
42.949
18.475
−17.308
1.00
13.54
B
C


ATOM
7405
O
ALA
B
361
42.932
18.866
−18.470
1.00
16.50
B
O


ATOM
7406
N
TRP
B
362
42.995
17.190
−16.991
1.00
13.97
B
N


ATOM
7407
CA
TRP
B
362
42.897
16.167
−18.043
1.00
12.24
B
C


ATOM
7408
CB
TRP
B
362
43.208
14.737
−17.493
1.00
13.20
B
C


ATOM
7409
CG
TRP
B
362
44.683
14.537
−17.560
1.00
13.43
B
C


ATOM
7410
CD1
TRP
B
362
45.605
14.660
−16.550
1.00
13.75
B
C


ATOM
7411
NE1
TRP
B
362
46.870
14.488
−17.041
1.00
14.85
B
N


ATOM
7412
CE2
TRP
B
362
46.844
14.249
−18.354
1.00
14.41
B
C


ATOM
7413
CD2
TRP
B
362
45.463
14.256
−18.762
1.00
13.93
B
C


ATOM
7414
CE3
TRP
B
362
45.151
14.037
−20.090
1.00
16.46
B
C


ATOM
7415
CZ3
TRP
B
362
46.186
13.803
−20.982
1.00
16.27
B
C


ATOM
7416
CH2
TRP
B
362
47.523
13.789
−20.551
1.00
15.56
B
C


ATOM
7417
CZ2
TRP
B
362
47.861
14.032
−19.238
1.00
17.59
B
C


ATOM
7418
C
TRP
B
362
41.599
16.203
−18.798
1.00
13.44
B
C


ATOM
7419
O
TRP
B
362
41.609
16.075
−20.027
1.00
14.23
B
O


ATOM
7420
N
ALA
B
363
40.478
16.402
−18.102
1.00
13.92
B
N


ATOM
7421
CA
ALA
B
363
39.158
16.453
−18.789
1.00
14.98
B
C


ATOM
7422
CB
ALA
B
363
37.987
16.519
−17.760
1.00
14.86
B
C


ATOM
7423
C
ALA
B
363
39.102
17.644
−19.755
1.00
14.71
B
C


ATOM
7424
O
ALA
B
363
38.680
17.507
−20.896
1.00
16.67
B
O


ATOM
7425
N
ASP
B
364
39.616
18.781
−19.317
1.00
15.71
B
N


ATOM
7426
CA
ASP
B
364
39.727
19.985
−20.160
1.00
18.73
B
C


ATOM
7427
CB
ASP
B
364
40.258
21.179
−19.337
1.00
19.66
B
C


ATOM
7428
CG
ASP
B
364
39.270
21.699
−18.300
1.00
23.82
B
C


ATOM
7429
OD1
ASP
B
364
38.093
21.286
−18.300
1.00
26.70
B
O


ATOM
7430
OD2
ASP
B
364
39.678
22.544
−17.462
1.00
26.89
B
O


ATOM
7431
C
ASP
B
364
40.622
19.783
−21.371
1.00
17.83
B
C


ATOM
7432
O
ASP
B
364
40.269
20.171
−22.474
1.00
15.74
B
O


ATOM
7433
N
LEU
B
365
41.771
19.117
−21.197
1.00
16.80
B
N


ATOM
7434
CA
LEU
B
365
42.614
18.812
−22.342
1.00
15.51
B
C


ATOM
7435
CB
LEU
B
365
43.949
18.247
−21.909
1.00
16.12
B
C


ATOM
7436
CG
LEU
B
365
44.923
17.884
−23.016
1.00
16.24
B
C


ATOM
7437
CD1
LEU
B
365
45.225
19.093
−23.874
1.00
17.38
B
C


ATOM
7438
CD2
LEU
B
365
46.195
17.331
−22.400
1.00
16.63
B
C


ATOM
7439
C
LEU
B
365
41.939
17.856
−23.329
1.00
16.21
B
C


ATOM
7440
O
LEU
B
365
41.941
18.110
−24.529
1.00
14.92
B
O


ATOM
7441
N
CYS
B
366
41.383
16.738
−22.842
1.00
15.29
B
N


ATOM
7442
CA
CYS
B
366
40.700
15.796
−23.756
1.00
15.91
B
C


ATOM
7443
CB
CYS
B
366
40.216
14.541
−23.025
1.00
17.87
B
C


ATOM
7444
SG
CYS
B
366
41.641
13.556
−22.499
1.00
21.04
B
S


ATOM
7445
C
CYS
B
366
39.542
16.464
−24.490
1.00
16.85
B
C


ATOM
7446
O
CYS
B
366
39.350
16.198
−25.671
1.00
14.79
B
O


ATOM
7447
N
ASN
B
367
38.817
17.362
−23.800
1.00
16.38
B
N


ATOM
7448
CA
ASN
B
367
37.761
18.157
−24.457
1.00
16.79
B
C


ATOM
7449
CB
ASN
B
367
36.849
18.878
−23.445
1.00
16.17
B
C


ATOM
7450
CG
ASN
B
367
35.713
17.976
−22.965
1.00
23.08
B
C


ATOM
7451
OD1
ASN
B
367
34.854
17.609
−23.760
1.00
21.90
B
O


ATOM
7452
ND2
ASN
B
367
35.738
17.563
−21.705
1.00
21.53
B
N


ATOM
7453
C
ASN
B
367
38.281
19.095
−25.545
1.00
17.97
B
C


ATOM
7454
O
ASN
B
367
37.636
19.231
−26.580
1.00
16.45
B
O


ATOM
7455
N
ALA
B
368
39.457
19.688
−25.352
1.00
18.77
B
N


ATOM
7456
CA
ALA
B
368
40.067
20.493
−26.405
1.00
16.37
B
C


ATOM
7457
CB
ALA
B
368
41.212
21.352
−25.878
1.00
13.38
B
C


ATOM
7458
C
ALA
B
368
40.513
19.603
−27.576
1.00
16.09
B
C


ATOM
7459
O
ALA
B
368
40.299
19.951
−28.736
1.00
13.68
B
O


ATOM
7460
N
PHE
B
369
41.105
18.445
−27.303
1.00
14.01
B
N


ATOM
7461
CA
PHE
B
369
41.413
17.512
−28.384
1.00
14.80
B
C


ATOM
7462
CB
PHE
B
369
42.087
16.242
−27.875
1.00
15.59
B
C


ATOM
7463
CG
PHE
B
369
43.516
16.425
−27.358
1.00
17.49
B
C


ATOM
7464
CD1
PHE
B
369
44.361
17.399
−27.860
1.00
18.60
B
C


ATOM
7465
CE1
PHE
B
369
45.671
17.512
−27.402
1.00
23.68
B
C


ATOM
7466
CZ
PHE
B
369
46.167
16.617
−26.442
1.00
19.70
B
C


ATOM
7467
CE2
PHE
B
369
45.347
15.633
−25.954
1.00
18.87
B
C


ATOM
7468
CD2
PHE
B
369
44.012
15.536
−26.419
1.00
20.51
B
C


ATOM
7469
C
PHE
B
369
40.162
17.065
−29.148
1.00
14.30
B
C


ATOM
7470
O
PHE
B
369
40.203
16.956
−30.379
1.00
12.66
B
O


ATOM
7471
N
LEU
B
370
39.085
16.757
−28.414
1.00
12.34
B
N


ATOM
7472
CA
LEU
B
370
37.833
16.326
−29.048
1.00
14.00
B
C


ATOM
7473
CB
LEU
B
370
36.811
15.941
−27.991
1.00
14.86
B
C


ATOM
7474
CG
LEU
B
370
35.478
15.350
−28.436
1.00
16.58
B
C


ATOM
7475
CD1
LEU
B
370
35.694
14.023
−29.171
1.00
15.46
B
C


ATOM
7476
CD2
LEU
B
370
34.467
15.214
−27.237
1.00
14.12
B
C


ATOM
7477
C
LEU
B
370
37.265
17.409
−29.973
1.00
14.60
B
C


ATOM
7478
O
LEU
B
370
36.800
17.127
−31.074
1.00
13.28
B
O


ATOM
7479
N
GLN
B
371
37.296
18.664
−29.515
1.00
14.38
B
N


ATOM
7480
CA
GLN
B
371
36.862
19.765
−30.364
1.00
12.58
B
C


ATOM
7481
CB
GLN
B
371
36.935
21.136
−29.624
1.00
13.91
B
C


ATOM
7482
CG
GLN
B
371
36.525
22.344
−30.491
1.00
15.05
B
C


ATOM
7483
CD
GLN
B
371
35.083
22.250
−30.923
1.00
21.08
B
C


ATOM
7484
OE1
GLN
B
371
34.747
21.704
−31.985
1.00
23.31
B
O


ATOM
7485
NE2
GLN
B
371
34.228
22.663
−30.062
1.00
15.07
B
N


ATOM
7486
C
GLN
B
371
37.659
19.793
−31.661
1.00
12.40
B
C


ATOM
7487
O
GLN
B
371
37.072
19.961
−32.712
1.00
16.60
B
O


ATOM
7488
N
GLU
B
372
38.977
19.620
−31.619
1.00
12.63
B
N


ATOM
7489
CA
GLU
B
372
39.777
19.560
−32.852
1.00
14.19
B
C


ATOM
7490
CB
GLU
B
372
41.297
19.436
−32.553
1.00
15.96
B
C


ATOM
7491
CG
GLU
B
372
41.827
20.751
−31.939
1.00
24.27
B
C


ATOM
7492
CD
GLU
B
372
43.324
20.791
−31.593
1.00
29.35
B
C


ATOM
7493
OE1
GLU
B
372
44.002
19.716
−31.433
1.00
38.38
B
O


ATOM
7494
OE2
GLU
B
372
43.808
21.931
−31.418
1.00
32.54
B
O


ATOM
7495
C
GLU
B
372
39.373
18.416
−33.787
1.00
14.37
B
C


ATOM
7496
O
GLU
B
372
39.254
18.619
−35.001
1.00
12.45
B
O


ATOM
7497
N
ALA
B
373
39.171
17.235
−33.203
1.00
15.48
B
N


ATOM
7498
CA
ALA
B
373
38.776
16.051
−33.966
1.00
16.23
B
C


ATOM
7499
CB
ALA
B
373
38.745
14.825
−33.070
1.00
15.65
B
C


ATOM
7500
C
ALA
B
373
37.422
16.287
−34.616
1.00
16.98
B
C


ATOM
7501
O
ALA
B
373
37.219
15.908
−35.767
1.00
13.16
B
O


ATOM
7502
N
LYS
B
374
36.502
16.946
−33.906
1.00
14.90
B
N


ATOM
7503
CA
LYS
B
374
35.180
17.201
−34.476
1.00
15.49
B
C


ATOM
7504
CB
LYS
B
374
34.195
17.686
−33.435
1.00
18.32
B
C


ATOM
7505
CG
LYS
B
374
33.717
16.658
−32.506
1.00
21.64
B
C


ATOM
7506
CD
LYS
B
374
32.830
17.267
−31.416
1.00
32.78
B
C


ATOM
7507
CE
LYS
B
374
32.172
16.151
−30.614
1.00
30.52
B
C


ATOM
7508
NZ
LYS
B
374
31.629
16.620
−29.322
1.00
27.14
B
N


ATOM
7509
C
LYS
B
374
35.254
18.204
−35.604
1.00
16.34
B
C


ATOM
7510
O
LYS
B
374
34.628
18.042
−36.663
1.00
13.85
B
O


ATOM
7511
N
TRP
B
375
36.001
19.278
−35.401
1.00
14.40
B
N


ATOM
7512
CA
TRP
B
375
36.148
20.242
−36.484
1.00
14.42
B
C


ATOM
7513
CB
TRP
B
375
37.068
21.370
−36.043
1.00
12.63
B
C


ATOM
7514
CG
TRP
B
375
36.412
22.448
−35.167
1.00
11.41
B
C


ATOM
7515
CD1
TRP
B
375
35.092
22.888
−35.154
1.00
13.11
B
C


ATOM
7516
NE1
TRP
B
375
34.950
23.935
−34.256
1.00
12.24
B
N


ATOM
7517
CE2
TRP
B
375
36.132
24.227
−33.685
1.00
12.65
B
C


ATOM
7518
CD2
TRP
B
375
37.116
23.330
−34.238
1.00
11.48
B
C


ATOM
7519
CE3
TRP
B
375
38.432
23.413
−33.799
1.00
13.19
B
C


ATOM
7520
CZ3
TRP
B
375
38.751
24.416
−32.864
1.00
14.17
B
C


ATOM
7521
CH2
TRP
B
375
37.772
25.253
−32.354
1.00
14.32
B
C


ATOM
7522
CZ2
TRP
B
375
36.463
25.181
−32.739
1.00
14.31
B
C


ATOM
7523
C
TRP
B
375
36.705
19.597
−37.727
1.00
18.06
B
C


ATOM
7524
O
TRP
B
375
36.269
19.888
−38.837
1.00
14.97
B
O


ATOM
7525
N
LEU
B
376
37.714
18.756
−37.541
1.00
20.64
B
N


ATOM
7526
CA
LEU
B
376
38.375
18.079
−38.660
1.00
21.54
B
C


ATOM
7527
CB
LEU
B
376
39.560
17.240
−38.181
1.00
23.28
B
C


ATOM
7528
CG
LEU
B
376
40.494
16.750
−39.299
1.00
34.36
B
C


ATOM
7529
CD1
LEU
B
376
41.152
17.935
−40.050
1.00
35.82
B
C


ATOM
7530
CD2
LEU
B
376
41.565
15.804
−38.729
1.00
33.84
B
C


ATOM
7531
C
LEU
B
376
37.379
17.181
−39.386
1.00
19.90
B
C


ATOM
7532
O
LEU
B
376
37.250
17.255
−40.603
1.00
20.49
B
O


ATOM
7533
N
TYR
B
377
36.639
16.402
−38.623
1.00
20.22
B
N


ATOM
7534
CA
TYR
B
377
35.662
15.486
−39.189
1.00
23.40
B
C


ATOM
7535
CB
TYR
B
377
35.028
14.624
−38.095
1.00
22.36
B
C


ATOM
7536
CG
TYR
B
377
33.973
13.666
−38.628
1.00
23.93
B
C


ATOM
7537
CD1
TYR
B
377
34.339
12.418
−39.158
1.00
30.84
B
C


ATOM
7538
CE1
TYR
B
377
33.379
11.541
−39.666
1.00
35.98
B
C


ATOM
7539
CZ
TYR
B
377
32.038
11.900
−39.640
1.00
38.89
B
C


ATOM
7540
OH
TYR
B
377
31.092
11.026
−40.129
1.00
48.41
B
O


ATOM
7541
CE2
TYR
B
377
31.643
13.121
−39.118
1.00
31.81
B
C


ATOM
7542
CD2
TYR
B
377
32.615
13.999
−38.611
1.00
31.71
B
C


ATOM
7543
C
TYR
B
377
34.551
16.219
−39.943
1.00
28.67
B
C


ATOM
7544
O
TYR
B
377
34.164
15.812
−41.028
1.00
20.24
B
O


ATOM
7545
N
ASN
B
378
34.026
17.290
−39.358
1.00
21.29
B
N


ATOM
7546
CA
ASN
B
378
32.915
18.021
−39.969
1.00
20.45
B
C


ATOM
7547
CB
ASN
B
378
32.084
18.701
−38.907
1.00
25.71
B
C


ATOM
7548
CG
ASN
B
378
31.390
17.736
−38.033
1.00
27.08
B
C


ATOM
7549
OD1
ASN
B
378
30.769
16.819
−38.512
1.00
29.20
B
O


ATOM
7550
ND2
ASN
B
378
31.467
17.941
−36.740
1.00
26.32
B
N


ATOM
7551
C
ASN
B
378
33.373
19.026
−41.002
1.00
18.69
B
C


ATOM
7552
O
ASN
B
378
32.564
19.718
−41.587
1.00
22.16
B
O


ATOM
7553
N
LYS
B
379
34.669
19.103
−41.244
1.00
17.02
B
N


ATOM
7554
CA
LYS
B
379
35.215
20.060
−42.185
1.00
24.24
B
C


ATOM
7555
CB
LYS
B
379
34.732
19.735
−43.601
1.00
27.41
B
C


ATOM
7556
CG
LYS
B
379
35.424
18.460
−44.172
1.00
39.50
B
C


ATOM
7557
CD
LYS
B
379
34.587
17.811
−45.284
1.00
48.10
B
C


ATOM
7558
CE
LYS
B
379
35.455
17.117
−46.317
1.00
62.70
B
C


ATOM
7559
NZ
LYS
B
379
36.439
16.191
−45.693
1.00
70.83
B
N


ATOM
7560
C
LYS
B
379
34.897
21.515
−41.780
1.00
21.35
B
C


ATOM
7561
O
LYS
B
379
34.567
22.351
−42.612
1.00
21.06
B
O


ATOM
7562
N
SER
B
380
34.986
21.795
−40.489
1.00
18.96
B
N


ATOM
7563
CA
SER
B
380
34.621
23.098
−39.988
1.00
20.28
B
C


ATOM
7564
CB
SER
B
380
34.452
23.048
−38.487
1.00
22.63
B
C


ATOM
7565
OG
SER
B
380
33.428
22.172
−38.167
1.00
17.13
B
O


ATOM
7566
C
SER
B
380
35.694
24.078
−40.322
1.00
19.57
B
C


ATOM
7567
O
SER
B
380
36.837
23.715
−40.449
1.00
17.94
B
O


ATOM
7568
N
THR
B
381
35.344
25.357
−40.390
1.00
19.95
B
N


ATOM
7569
CA
THR
B
381
36.314
26.389
−40.691
1.00
14.29
B
C


ATOM
7570
CB
THR
B
381
35.996
26.981
−42.067
1.00
14.00
B
C


ATOM
7571
OG1
THR
B
381
34.621
27.374
−42.061
1.00
17.82
B
O


ATOM
7572
CG2
THR
B
381
36.214
25.883
−43.219
1.00
20.33
B
C


ATOM
7573
C
THR
B
381
36.179
27.510
−39.624
1.00
17.48
B
C


ATOM
7574
O
THR
B
381
35.743
28.602
−39.943
1.00
14.56
B
O


ATOM
7575
N
PRO
B
382
36.518
27.225
−38.365
1.00
14.21
B
N


ATOM
7576
CA
PRO
B
382
36.366
28.257
−37.335
1.00
16.47
B
C


ATOM
7577
CB
PRO
B
382
36.777
27.532
−36.051
1.00
15.71
B
C


ATOM
7578
CG
PRO
B
382
37.580
26.381
−36.501
1.00
16.62
B
C


ATOM
7579
CD
PRO
B
382
37.117
25.993
−37.834
1.00
15.97
B
C


ATOM
7580
C
PRO
B
382
37.233
29.492
−37.524
1.00
14.79
B
C


ATOM
7581
O
PRO
B
382
38.319
29.419
−38.086
1.00
14.02
B
O


ATOM
7582
N
THR
B
383
36.786
30.616
−36.968
1.00
12.94
B
N


ATOM
7583
CA
THR
B
383
37.565
31.826
−36.989
1.00
11.18
B
C


ATOM
7584
CB
THR
B
383
36.805
33.013
−36.303
1.00
10.38
B
C


ATOM
7585
OG1
THR
B
383
36.684
32.722
−34.903
1.00
10.91
B
O


ATOM
7586
CG2
THR
B
383
35.411
33.234
−36.931
1.00
8.96
B
C


ATOM
7587
C
THR
B
383
38.852
31.604
−36.256
1.00
12.11
B
C


ATOM
7588
O
THR
B
383
38.966
30.692
−35.402
1.00
10.33
B
O


ATOM
7589
N
PHE
B
384
39.817
32.490
−36.500
1.00
10.85
B
N


ATOM
7590
CA
PHE
B
384
41.054
32.439
−35.731
1.00
11.66
B
C


ATOM
7591
CB
PHE
B
384
42.020
33.553
−36.132
1.00
12.97
B
C


ATOM
7592
CG
PHE
B
384
43.236
33.622
−35.252
1.00
11.75
B
C


ATOM
7593
CD1
PHE
B
384
44.344
32.803
−35.508
1.00
12.41
B
C


ATOM
7594
CE1
PHE
B
384
45.424
32.806
−34.679
1.00
13.91
B
C


ATOM
7595
CZ
PHE
B
384
45.456
33.656
−33.552
1.00
13.96
B
C


ATOM
7596
CE2
PHE
B
384
44.372
34.464
−33.291
1.00
13.02
B
C


ATOM
7597
CD2
PHE
B
384
43.264
34.449
−34.159
1.00
12.10
B
C


ATOM
7598
C
PHE
B
384
40.811
32.476
−34.213
1.00
13.50
B
C


ATOM
7599
O
PHE
B
384
41.417
31.689
−33.464
1.00
11.60
B
O


ATOM
7600
N
ASP
B
385
39.985
33.410
−33.735
1.00
11.08
B
N


ATOM
7601
CA
ASP
B
385
39.685
33.468
−32.292
1.00
11.99
B
C


ATOM
7602
CB
ASP
B
385
38.682
34.576
−31.961
1.00
13.98
B
C


ATOM
7603
CG
ASP
B
385
39.316
35.984
−32.088
1.00
16.22
B
C


ATOM
7604
OD1
ASP
B
385
40.548
36.066
−32.040
1.00
17.51
B
O


ATOM
7605
OD2
ASP
B
385
38.610
36.967
−32.290
1.00
16.80
B
O


ATOM
7606
C
ASP
B
385
39.151
32.169
−31.722
1.00
10.77
B
C


ATOM
7607
O
ASP
B
385
39.547
31.776
−30.631
1.00
11.38
B
O


ATOM
7608
N
ASP
B
386
38.210
31.545
−32.396
1.00
11.91
B
N


ATOM
7609
CA
ASP
B
386
37.682
30.240
−31.921
1.00
12.63
B
C


ATOM
7610
CB
ASP
B
386
36.481
29.824
−32.750
1.00
14.45
B
C


ATOM
7611
CG
ASP
B
386
35.210
30.550
−32.337
1.00
25.95
B
C


ATOM
7612
OD1
ASP
B
386
35.272
31.290
−31.342
1.00
26.16
B
O


ATOM
7613
OD2
ASP
B
386
34.173
30.382
−33.014
1.00
26.70
B
O


ATOM
7614
C
ASP
B
386
38.722
29.134
−31.997
1.00
13.21
B
C


ATOM
7615
O
ASP
B
386
38.902
28.343
−31.042
1.00
14.69
B
O


ATOM
7616
N
TYR
B
387
39.412
29.058
−33.132
1.00
12.07
B
N


ATOM
7617
CA
TYR
B
387
40.425
27.998
−33.316
1.00
12.49
B
C


ATOM
7618
CB
TYR
B
387
41.030
28.016
−34.718
1.00
16.80
B
C


ATOM
7619
CG
TYR
B
387
42.023
26.899
−34.880
1.00
20.99
B
C


ATOM
7620
CD1
TYR
B
387
41.564
25.606
−35.143
1.00
23.33
B
C


ATOM
7621
CE1
TYR
B
387
42.427
24.550
−35.298
1.00
28.23
B
C


ATOM
7622
CZ
TYR
B
387
43.781
24.757
−35.146
1.00
27.53
B
C


ATOM
7623
OH
TYR
B
387
44.588
23.648
−35.278
1.00
30.12
B
O


ATOM
7624
CE2
TYR
B
387
44.288
26.017
−34.851
1.00
22.49
B
C


ATOM
7625
CD2
TYR
B
387
43.382
27.103
−34.720
1.00
22.96
B
C


ATOM
7626
C
TYR
B
387
41.540
28.122
−32.305
1.00
14.93
B
C


ATOM
7627
O
TYR
B
387
41.889
27.138
−31.572
1.00
12.65
B
O


ATOM
7628
N
PHE
B
388
42.079
29.332
−32.216
1.00
14.05
B
N


ATOM
7629
CA
PHE
B
388
43.221
29.590
−31.324
1.00
12.82
B
C


ATOM
7630
CB
PHE
B
388
43.815
31.007
−31.576
1.00
14.30
B
C


ATOM
7631
CG
PHE
B
388
45.008
31.331
−30.729
1.00
12.87
B
C


ATOM
7632
CD1
PHE
B
388
46.168
30.597
−30.846
1.00
15.58
B
C


ATOM
7633
CE1
PHE
B
388
47.275
30.848
−30.003
1.00
18.48
B
C


ATOM
7634
CZ
PHE
B
388
47.233
31.905
−29.073
1.00
15.76
B
C


ATOM
7635
CE2
PHE
B
388
46.056
32.630
−28.933
1.00
19.27
B
C


ATOM
7636
CD2
PHE
B
388
44.954
32.352
−29.774
1.00
17.96
B
C


ATOM
7637
C
PHE
B
388
42.787
29.430
−29.870
1.00
12.87
B
C


ATOM
7638
O
PHE
B
388
43.571
28.959
−29.055
1.00
14.74
B
O


ATOM
7639
N
GLY
B
389
41.589
29.903
−29.525
1.00
15.26
B
N


ATOM
7640
CA
GLY
B
389
41.008
29.764
−28.133
1.00
14.49
B
C


ATOM
7641
C
GLY
B
389
41.056
28.311
−27.654
1.00
14.82
B
C


ATOM
7642
O
GLY
B
389
41.467
28.020
−26.530
1.00
18.64
B
O


ATOM
7643
N
ASN
B
390
40.697
27.379
−28.541
1.00
17.04
B
N


ATOM
7644
CA
ASN
B
390
40.862
25.959
−28.269
1.00
13.61
B
C


ATOM
7645
CB
ASN
B
390
39.942
25.148
−29.207
1.00
15.14
B
C


ATOM
7646
CG
ASN
B
390
39.832
23.706
−28.801
1.00
14.66
B
C


ATOM
7647
OD1
ASN
B
390
39.156
23.393
−27.874
1.00
14.99
B
O


ATOM
7648
ND2
ASN
B
390
40.470
22.825
−29.523
1.00
11.39
B
N


ATOM
7649
C
ASN
B
390
42.333
25.450
−28.372
1.00
15.73
B
C


ATOM
7650
O
ASN
B
390
42.754
24.625
−27.566
1.00
13.70
B
O


ATOM
7651
N
ALA
B
391
43.093
25.932
−29.361
1.00
14.07
B
N


ATOM
7652
CA
ALA
B
391
44.421
25.428
−29.637
1.00
14.55
B
C


ATOM
7653
CB
ALA
B
391
44.909
25.965
−31.011
1.00
12.31
B
C


ATOM
7654
C
ALA
B
391
45.432
25.719
−28.528
1.00
17.05
B
C


ATOM
7655
O
ALA
B
391
46.364
24.922
−28.297
1.00
14.56
B
O


ATOM
7656
N
TRP
B
392
45.317
26.860
−27.841
1.00
14.34
B
N


ATOM
7657
CA
TRP
B
392
46.234
27.067
−26.731
1.00
16.32
B
C


ATOM
7658
CB
TRP
B
392
46.401
28.542
−26.313
1.00
16.63
B
C


ATOM
7659
CG
TRP
B
392
45.226
29.273
−25.710
1.00
14.95
B
C


ATOM
7660
CD1
TRP
B
392
44.435
30.228
−26.314
1.00
14.59
B
C


ATOM
7661
NE1
TRP
B
392
43.499
30.687
−25.468
1.00
16.87
B
N


ATOM
7662
CE2
TRP
B
392
43.655
30.167
−24.259
1.00
19.26
B
C


ATOM
7663
CD2
TRP
B
392
44.745
29.204
−24.349
1.00
13.74
B
C


ATOM
7664
CE3
TRP
B
392
45.123
28.505
−23.221
1.00
16.86
B
C


ATOM
7665
CZ3
TRP
B
392
44.399
28.704
−22.048
1.00
19.49
B
C


ATOM
7666
CH2
TRP
B
392
43.324
29.607
−21.992
1.00
21.16
B
C


ATOM
7667
CZ2
TRP
B
392
42.933
30.360
−23.096
1.00
20.28
B
C


ATOM
7668
C
TRP
B
392
45.969
26.147
−25.577
1.00
16.74
B
C


ATOM
7669
O
TRP
B
392
46.896
25.820
−24.849
1.00
16.66
B
O


ATOM
7670
N
LYS
B
393
44.732
25.697
−25.403
1.00
13.97
B
N


ATOM
7671
CA
ALYS
B
393
44.432
24.701
−24.391
0.50
16.29
B
C


ATOM
7672
CA
BLYS
B
393
44.409
24.679
−24.402
0.50
16.84
B
C


ATOM
7673
CB
ALYS
B
393
42.925
24.689
−24.066
0.50
17.23
B
C


ATOM
7674
CB
BLYS
B
393
42.892
24.547
−24.178
0.50
18.43
B
C


ATOM
7675
CG
ALYS
B
393
42.407
26.061
−23.590
0.50
19.87
B
C


ATOM
7676
CG
BLYS
B
393
42.227
25.745
−23.524
0.50
22.26
B
C


ATOM
7677
CD
ALYS
B
393
40.926
26.081
−23.145
0.50
19.59
B
C


ATOM
7678
CD
BLYS
B
393
40.698
25.675
−23.664
0.50
22.54
B
C


ATOM
7679
CE
ALYS
B
393
40.439
27.520
−22.818
0.50
24.24
B
C


ATOM
7680
CE
BLYS
B
393
40.020
26.968
−23.255
0.50
30.19
B
C


ATOM
7681
NZ
ALYS
B
393
40.026
28.379
−24.047
0.50
26.04
B
N


ATOM
7682
NZ
BLYS
B
393
39.565
27.013
−21.834
0.50
26.38
B
N


ATOM
7683
C
LYS
B
393
44.927
23.309
−24.837
1.00
16.19
B
C


ATOM
7684
O
LYS
B
393
45.524
22.588
−24.050
1.00
17.75
B
O


ATOM
7685
N
SER
B
394
44.690
22.967
−26.097
1.00
14.65
B
N


ATOM
7686
CA
SER
B
394
45.108
21.697
−26.664
1.00
16.05
B
C


ATOM
7687
CB
SER
B
394
44.487
21.443
−28.053
1.00
15.63
B
C


ATOM
7688
OG
SER
B
394
45.175
22.134
−29.095
1.00
16.43
B
O


ATOM
7689
C
SER
B
394
46.603
21.561
−26.781
1.00
16.75
B
C


ATOM
7690
O
SER
B
394
47.069
20.463
−26.981
1.00
14.84
B
O


ATOM
7691
N
SER
B
395
47.351
22.660
−26.683
1.00
17.02
B
N


ATOM
7692
CA
SER
B
395
48.819
22.574
−26.593
1.00
17.94
B
C


ATOM
7693
CB
SER
B
395
49.437
23.941
−26.370
1.00
17.30
B
C


ATOM
7694
OG
SER
B
395
49.134
24.510
−25.088
1.00
13.55
B
O


ATOM
7695
C
SER
B
395
49.291
21.677
−25.431
1.00
15.76
B
C


ATOM
7696
O
SER
B
395
50.376
21.123
−25.489
1.00
16.76
B
O


ATOM
7697
N
SER
B
396
48.489
21.642
−24.373
1.00
13.76
B
N


ATOM
7698
CA
SER
B
396
48.762
21.022
−23.076
1.00
17.04
B
C


ATOM
7699
CB
SER
B
396
49.445
19.653
−23.161
1.00
17.48
B
C


ATOM
7700
OG
SER
B
396
50.853
19.741
−23.382
1.00
15.38
B
O


ATOM
7701
C
SER
B
396
49.534
21.961
−22.140
1.00
17.50
B
C


ATOM
7702
O
SER
B
396
49.784
21.614
−21.002
1.00
16.88
B
O


ATOM
7703
N
GLY
B
397
49.874
23.171
−22.606
1.00
16.26
B
N


ATOM
7704
CA
GLY
B
397
50.504
24.184
−21.738
1.00
14.34
B
C


ATOM
7705
C
GLY
B
397
49.824
24.358
−20.397
1.00
15.51
B
C


ATOM
7706
O
GLY
B
397
50.472
24.259
−19.348
1.00
16.30
B
O


ATOM
7707
N
PRO
B
398
48.508
24.565
−20.401
1.00
14.57
B
N


ATOM
7708
CA
PRO
B
398
47.852
24.807
−19.120
1.00
17.72
B
C


ATOM
7709
CB
PRO
B
398
46.417
25.177
−19.517
1.00
18.32
B
C


ATOM
7710
CG
PRO
B
398
46.618
25.755
−20.932
1.00
17.69
B
C


ATOM
7711
CD
PRO
B
398
47.646
24.918
−21.540
1.00
18.38
B
C


ATOM
7712
C
PRO
B
398
47.893
23.595
−18.210
1.00
16.14
B
C


ATOM
7713
O
PRO
B
398
48.128
23.739
−17.006
1.00
19.03
B
O


ATOM
7714
N
LEU
B
399
47.734
22.406
−18.786
1.00
17.00
B
N


ATOM
7715
CA
LEU
B
399
47.787
21.185
−17.997
1.00
16.02
B
C


ATOM
7716
CB
LEU
B
399
47.384
19.950
−18.846
1.00
14.75
B
C


ATOM
7717
CG
LEU
B
399
47.440
18.620
−18.074
1.00
15.21
B
C


ATOM
7718
CD1
LEU
B
399
46.325
17.703
−18.504
1.00
15.91
B
C


ATOM
7719
CD2
LEU
B
399
48.798
17.906
−18.311
1.00
14.96
B
C


ATOM
7720
C
LEU
B
399
49.187
21.015
−17.402
1.00
17.23
B
C


ATOM
7721
O
LEU
B
399
49.334
20.686
−16.233
1.00
15.66
B
O


ATOM
7722
N
GLN
B
400
50.222
21.227
−18.204
1.00
14.25
B
N


ATOM
7723
CA
GLN
B
400
51.571
21.062
−17.692
1.00
16.46
B
C


ATOM
7724
CB
GLN
B
400
52.571
21.338
−18.804
1.00
17.14
B
C


ATOM
7725
CG
GLN
B
400
52.574
20.251
−19.891
1.00
22.36
B
C


ATOM
7726
CD
GLN
B
400
53.679
20.470
−20.915
1.00
23.80
B
C


ATOM
7727
OE1
GLN
B
400
54.753
20.953
−20.570
1.00
26.17
B
O


ATOM
7728
NE2
GLN
B
400
53.403
20.144
−22.200
1.00
21.26
B
N


ATOM
7729
C
GLN
B
400
51.831
21.996
−16.505
1.00
16.03
B
C


ATOM
7730
O
GLN
B
400
52.461
21.638
−15.492
1.00
17.98
B
O


ATOM
7731
N
LEU
B
401
51.323
23.202
−16.612
1.00
16.36
B
N


ATOM
7732
CA
LEU
B
401
51.580
24.189
−15.573
1.00
16.93
B
C


ATOM
7733
CB
LEU
B
401
51.406
25.621
−16.137
1.00
16.62
B
C


ATOM
7734
CG
LEU
B
401
52.510
26.026
−17.139
1.00
16.58
B
C


ATOM
7735
CD1
LEU
B
401
52.218
27.399
−17.812
1.00
16.43
B
C


ATOM
7736
CD2
LEU
B
401
53.956
25.978
−16.555
1.00
17.12
B
C


ATOM
7737
C
LEU
B
401
50.738
23.940
−14.323
1.00
16.36
B
C


ATOM
7738
O
LEU
B
401
51.161
24.321
−13.223
1.00
17.21
B
O


ATOM
7739
N
VAL
B
402
49.560
23.291
−14.455
1.00
15.44
B
N


ATOM
7740
CA
VAL
B
402
48.783
22.891
−13.271
1.00
16.82
B
C


ATOM
7741
CB
VAL
B
402
47.409
22.358
−13.638
1.00
19.24
B
C


ATOM
7742
CG1
VAL
B
402
46.785
21.555
−12.475
1.00
21.42
B
C


ATOM
7743
CG2
VAL
B
402
46.523
23.505
−14.109
1.00
18.39
B
C


ATOM
7744
C
VAL
B
402
49.611
21.831
−12.539
1.00
17.32
B
C


ATOM
7745
O
VAL
B
402
49.825
21.939
−11.352
1.00
19.90
B
O


ATOM
7746
N
PHE
B
403
50.152
20.853
−13.265
1.00
16.42
B
N


ATOM
7747
CA
PHE
B
403
51.010
19.849
−12.627
1.00
15.92
B
C


ATOM
7748
CB
PHE
B
403
51.306
18.711
−13.596
1.00
16.25
B
C


ATOM
7749
CG
PHE
B
403
50.205
17.685
−13.654
1.00
18.43
B
C


ATOM
7750
CD1
PHE
B
403
49.101
17.874
−14.470
1.00
16.84
B
C


ATOM
7751
CE1
PHE
B
403
48.073
16.956
−14.498
1.00
17.95
B
C


ATOM
7752
CZ
PHE
B
403
48.150
15.813
−13.720
1.00
17.55
B
C


ATOM
7753
CE2
PHE
B
403
49.274
15.600
−12.930
1.00
17.75
B
C


ATOM
7754
CD2
PHE
B
403
50.281
16.534
−12.888
1.00
16.36
B
C


ATOM
7755
C
PHE
B
403
52.296
20.451
−12.034
1.00
20.51
B
C


ATOM
7756
O
PHE
B
403
52.710
20.097
−10.921
1.00
18.15
B
O


ATOM
7757
N
ALA
B
404
52.869
21.427
−12.731
1.00
18.27
B
N


ATOM
7758
CA
ALA
B
404
54.068
22.116
−12.256
1.00
21.22
B
C


ATOM
7759
CB
ALA
B
404
54.650
23.019
−13.338
1.00
19.30
B
C


ATOM
7760
C
ALA
B
404
53.763
22.906
−10.986
1.00
19.19
B
C


ATOM
7761
O
ALA
B
404
54.575
22.905
−10.069
1.00
18.68
B
O


ATOM
7762
N
TYR
B
405
52.598
23.544
−10.913
1.00
17.24
B
N


ATOM
7763
CA
TYR
B
405
52.178
24.284
−9.704
1.00
19.01
B
C


ATOM
7764
CB
TYR
B
405
50.737
24.823
−9.830
1.00
17.43
B
C


ATOM
7765
CG
TYR
B
405
50.197
25.462
−8.549
1.00
18.45
B
C


ATOM
7766
CD1
TYR
B
405
50.452
26.809
−8.228
1.00
18.58
B
C


ATOM
7767
CE1
TYR
B
405
49.964
27.364
−7.046
1.00
21.18
B
C


ATOM
7768
CZ
TYR
B
405
49.243
26.585
−6.174
1.00
27.35
B
C


ATOM
7769
OH
TYR
B
405
48.769
27.080
−4.969
1.00
24.53
B
O


ATOM
7770
CE2
TYR
B
405
48.984
25.269
−6.489
1.00
26.69
B
C


ATOM
7771
CD2
TYR
B
405
49.456
24.725
−7.653
1.00
19.95
B
C


ATOM
7772
C
TYR
B
405
52.293
23.391
−8.462
1.00
22.99
B
C


ATOM
7773
O
TYR
B
405
52.935
23.745
−7.461
1.00
19.04
B
O


ATOM
7774
N
PHE
B
406
51.698
22.206
−8.526
1.00
21.16
B
N


ATOM
7775
CA
PHE
B
406
51.714
21.337
−7.356
1.00
19.33
B
C


ATOM
7776
CB
PHE
B
406
50.673
20.225
−7.474
1.00
20.00
B
C


ATOM
7777
CG
PHE
B
406
49.263
20.733
−7.528
1.00
18.99
B
C


ATOM
7778
CD1
PHE
B
406
48.657
21.211
−6.385
1.00
16.83
B
C


ATOM
7779
CE1
PHE
B
406
47.361
21.704
−6.425
1.00
17.64
B
C


ATOM
7780
CZ
PHE
B
406
46.658
21.696
−7.624
1.00
21.27
B
C


ATOM
7781
CE2
PHE
B
406
47.266
21.186
−8.771
1.00
18.93
B
C


ATOM
7782
CD2
PHE
B
406
48.548
20.734
−8.718
1.00
18.22
B
C


ATOM
7783
C
PHE
B
406
53.081
20.762
−7.044
1.00
24.99
B
C


ATOM
7784
O
PHE
B
406
53.328
20.393
−5.905
1.00
22.61
B
O


ATOM
7785
N
ALA
B
407
53.970
20.673
−8.029
1.00
21.48
B
N


ATOM
7786
CA
ALA
B
407
55.318
20.191
−7.778
1.00
21.13
B
C


ATOM
7787
CB
ALA
B
407
55.844
19.442
−8.997
1.00
17.54
B
C


ATOM
7788
C
ALA
B
407
56.313
21.275
−7.358
1.00
26.36
B
C


ATOM
7789
O
ALA
B
407
57.410
20.940
−6.889
1.00
31.08
B
O


ATOM
7790
N
VAL
B
408
55.977
22.552
−7.546
1.00
29.15
B
N


ATOM
7791
CA
VAL
B
408
56.893
23.616
−7.148
1.00
25.79
B
C


ATOM
7792
CB
VAL
B
408
57.283
24.618
−8.287
1.00
29.33
B
C


ATOM
7793
CG1
VAL
B
408
57.817
23.898
−9.520
1.00
26.70
B
C


ATOM
7794
CG2
VAL
B
408
56.148
25.509
−8.613
1.00
29.67
B
C


ATOM
7795
C
VAL
B
408
56.425
24.448
−5.969
1.00
28.18
B
C


ATOM
7796
O
VAL
B
408
57.251
25.061
−5.355
1.00
30.90
B
O


ATOM
7797
N
VAL
B
409
55.134
24.495
−5.658
1.00
28.79
B
N


ATOM
7798
CA
VAL
B
409
54.639
25.344
−4.572
1.00
35.25
B
C


ATOM
7799
CB
VAL
B
409
53.292
26.011
−4.958
1.00
34.13
B
C


ATOM
7800
CG1
VAL
B
409
52.508
26.477
−3.738
1.00
38.15
B
C


ATOM
7801
CG2
VAL
B
409
53.545
27.173
−5.914
1.00
33.80
B
C


ATOM
7802
C
VAL
B
409
54.470
24.512
−3.293
1.00
43.84
B
C


ATOM
7803
O
VAL
B
409
53.712
23.547
−3.285
1.00
49.99
B
O


ATOM
7804
N
GLN
B
410
55.147
24.914
−2.218
1.00
46.58
B
N


ATOM
7805
CA
GLN
B
410
55.111
24.168
−0.939
1.00
51.15
B
C


ATOM
7806
CB
GLN
B
410
56.196
24.665
0.017
1.00
62.44
B
C


ATOM
7807
CG
GLN
B
410
57.608
24.223
−0.368
1.00
85.53
B
C


ATOM
7808
CD
GLN
B
410
58.693
24.878
0.483
1.00
98.51
B
C


ATOM
7809
OE1
GLN
B
410
58.437
25.837
1.212
1.00
115.04
B
O


ATOM
7810
NE2
GLN
B
410
59.913
24.361
0.387
1.00
96.93
B
N


ATOM
7811
C
GLN
B
410
53.746
24.235
−0.259
1.00
34.26
B
C


ATOM
7812
O
GLN
B
410
53.184
23.204
0.136
1.00
41.14
B
O


ATOM
7813
N
ASN
B
411
53.193
25.440
−0.170
1.00
36.04
B
N


ATOM
7814
CA
ASN
B
411
51.879
25.655
0.431
1.00
36.24
B
C


ATOM
7815
CB
ASN
B
411
51.993
26.656
1.586
1.00
39.41
B
C


ATOM
7816
CG
ASN
B
411
52.858
26.121
2.700
1.00
44.31
B
C


ATOM
7817
OD1
ASN
B
411
54.002
26.539
2.868
1.00
52.88
B
O


ATOM
7818
ND2
ASN
B
411
52.345
25.135
3.414
1.00
37.57
B
N


ATOM
7819
C
ASN
B
411
50.851
26.127
−0.577
1.00
34.78
B
C


ATOM
7820
O
ASN
B
411
50.806
27.315
−0.947
1.00
33.95
B
O


ATOM
7821
N
ILE
B
412
49.989
25.202
−0.974
1.00
32.83
B
N


ATOM
7822
CA
ILE
B
412
49.002
25.486
−1.986
1.00
30.16
B
C


ATOM
7823
CB
ILE
B
412
48.372
24.173
−2.580
1.00
39.38
B
C


ATOM
7824
CG1
ILE
B
412
47.327
23.547
−1.664
1.00
36.76
B
C


ATOM
7825
CD1
ILE
B
412
46.506
22.420
−2.347
1.00
30.79
B
C


ATOM
7826
CG2
ILE
B
412
49.455
23.141
−2.892
1.00
39.51
B
C


ATOM
7827
C
ILE
B
412
47.961
26.397
−1.377
1.00
33.47
B
C


ATOM
7828
O
ILE
B
412
47.693
26.325
−0.182
1.00
30.49
B
O


ATOM
7829
N
LYS
B
413
47.367
27.250
−2.190
1.00
28.56
B
N


ATOM
7830
CA
LYS
B
413
46.293
28.111
−1.728
1.00
31.18
B
C


ATOM
7831
CB
LYS
B
413
46.742
29.567
−1.724
1.00
33.98
B
C


ATOM
7832
CG
LYS
B
413
47.863
29.824
−0.747
1.00
39.78
B
C


ATOM
7833
CD
LYS
B
413
48.596
31.111
−1.062
1.00
50.58
B
C


ATOM
7834
CE
LYS
B
413
49.785
31.288
−0.148
1.00
49.79
B
C


ATOM
7835
NZ
LYS
B
413
50.396
32.600
−0.413
1.00
53.85
B
N


ATOM
7836
C
LYS
B
413
45.077
27.957
−2.597
1.00
31.66
B
C


ATOM
7837
O
LYS
B
413
45.172
27.893
−3.818
1.00
29.12
B
O


ATOM
7838
N
LYS
B
414
43.929
27.915
−1.936
1.00
28.79
B
N


ATOM
7839
CA
LYS
B
414
42.638
27.829
−2.568
1.00
40.86
B
C


ATOM
7840
CB
LYS
B
414
41.532
28.075
−1.524
1.00
51.07
B
C


ATOM
7841
CG
LYS
B
414
40.102
28.244
−2.085
1.00
66.21
B
C


ATOM
7842
CD
LYS
B
414
39.612
27.042
−2.909
1.00
79.46
B
C


ATOM
7843
CE
LYS
B
414
39.427
25.788
−2.073
1.00
89.72
B
C


ATOM
7844
NZ
LYS
B
414
38.431
25.988
−0.988
1.00
98.92
B
N


ATOM
7845
C
LYS
B
414
42.469
28.799
−3.733
1.00
32.82
B
C


ATOM
7846
O
LYS
B
414
42.016
28.394
−4.798
1.00
24.44
B
O


ATOM
7847
N
GLU
B
415
42.780
30.076
−3.537
1.00
27.69
B
N


ATOM
7848
CA
GLU
B
415
42.501
31.041
−4.589
1.00
32.63
B
C


ATOM
7849
CB
GLU
B
415
42.482
32.489
−4.055
1.00
36.78
B
C


ATOM
7850
CG
GLU
B
415
43.682
33.333
−4.394
1.00
59.26
B
C


ATOM
7851
CD
GLU
B
415
43.532
34.800
−3.967
1.00
65.67
B
C


ATOM
7852
OE1
GLU
B
415
42.448
35.405
−4.161
1.00
47.51
B
O


ATOM
7853
OE2
GLU
B
415
44.530
35.348
−3.460
1.00
66.03
B
O


ATOM
7854
C
GLU
B
415
43.453
30.855
−5.800
1.00
22.84
B
C


ATOM
7855
O
GLU
B
415
43.078
31.143
−6.917
1.00
28.14
B
O


ATOM
7856
N
GLU
B
416
44.675
30.412
−5.557
1.00
23.46
B
N


ATOM
7857
CA
GLU
B
416
45.592
30.094
−6.634
1.00
26.32
B
C


ATOM
7858
CB
GLU
B
416
46.983
29.771
−6.092
1.00
27.26
B
C


ATOM
7859
CG
GLU
B
416
47.672
30.981
−5.449
1.00
33.55
B
C


ATOM
7860
CD
GLU
B
416
49.106
30.692
−5.016
1.00
35.88
B
C


ATOM
7861
OE1
GLU
B
416
49.435
29.528
−4.697
1.00
33.22
B
O


ATOM
7862
OE2
GLU
B
416
49.912
31.637
−4.995
1.00
44.51
B
O


ATOM
7863
C
GLU
B
416
45.052
28.936
−7.454
1.00
27.30
B
C


ATOM
7864
O
GLU
B
416
44.998
29.040
−8.661
1.00
26.85
B
O


ATOM
7865
N
ILE
B
417
44.611
27.870
−6.784
1.00
31.08
B
N


ATOM
7866
CA
AILE
B
417
44.073
26.700
−7.488
0.50
30.71
B
C


ATOM
7867
CA
BILE
B
417
44.016
26.696
−7.432
0.50
31.36
B
C


ATOM
7868
CB
AILE
B
417
43.699
25.514
−6.566
0.50
31.05
B
C


ATOM
7869
CB
BILE
B
417
43.501
25.688
−6.388
0.50
32.70
B
C


ATOM
7870
CG1
AILE
B
417
44.820
25.146
−5.612
0.50
28.12
B
C


ATOM
7871
CG1
BILE
B
417
44.676
25.067
−5.670
0.50
27.91
B
C


ATOM
7872
CD1
AILE
B
417
44.335
24.332
−4.406
0.50
26.96
B
C


ATOM
7873
CD1
BILE
B
417
45.850
24.919
−6.546
0.50
24.89
B
C


ATOM
7874
CG2
AILE
B
417
43.401
24.278
−7.415
0.50
33.62
B
C


ATOM
7875
CG2
BILE
B
417
42.665
24.601
−7.051
0.50
42.31
B
C


ATOM
7876
C
ILE
B
417
42.841
27.083
−8.286
1.00
27.12
B
C


ATOM
7877
O
ILE
B
417
42.709
26.665
−9.426
1.00
30.48
B
O


ATOM
7878
N
GLU
B
418
41.962
27.891
−7.705
1.00
31.07
B
N


ATOM
7879
CA
GLU
B
418
40.757
28.339
−8.415
1.00
34.04
B
C


ATOM
7880
CB
GLU
B
418
39.864
29.176
−7.499
1.00
40.60
B
C


ATOM
7881
CG
GLU
B
418
39.146
28.377
−6.415
1.00
52.16
B
C


ATOM
7882
CD
GLU
B
418
38.224
29.253
−5.553
1.00
61.73
B
C


ATOM
7883
OE1
GLU
B
418
38.642
30.362
−5.126
1.00
53.45
B
O


ATOM
7884
OE2
GLU
B
418
37.080
28.821
−5.297
1.00
78.65
B
O


ATOM
7885
C
GLU
B
418
41.102
29.165
−9.644
1.00
31.84
B
C


ATOM
7886
O
GLU
B
418
40.425
29.087
−10.676
1.00
27.15
B
O


ATOM
7887
N
ASN
B
419
42.141
29.984
−9.540
1.00
23.18
B
N


ATOM
7888
CA
ASN
B
419
42.545
30.753
−10.703
1.00
28.59
B
C


ATOM
7889
CB
ASN
B
419
43.419
31.902
−10.275
1.00
26.05
B
C


ATOM
7890
CG
ASN
B
419
42.584
33.055
−9.825
1.00
32.56
B
C


ATOM
7891
OD1
ASN
B
419
42.071
33.785
−10.651
1.00
32.13
B
O


ATOM
7892
ND2
ASN
B
419
42.360
33.167
−8.515
1.00
26.49
B
N


ATOM
7893
C
ASN
B
419
43.183
29.953
−11.842
1.00
21.02
B
C


ATOM
7894
O
ASN
B
419
42.954
30.273
−13.021
1.00
23.89
B
O


ATOM
7895
N
LEU
B
420
43.965
28.933
−11.482
1.00
21.54
B
N


ATOM
7896
CA
LEU
B
420
44.461
27.967
−12.448
1.00
26.65
B
C


ATOM
7897
CB
LEU
B
420
45.247
26.839
−11.769
1.00
25.17
B
C


ATOM
7898
CG
LEU
B
420
46.577
27.175
−11.077
1.00
32.36
B
C


ATOM
7899
CD1
LEU
B
420
47.067
25.963
−10.301
1.00
30.40
B
C


ATOM
7900
CD2
LEU
B
420
47.619
27.666
−12.058
1.00
28.07
B
C


ATOM
7901
C
LEU
B
420
43.300
27.353
−13.238
1.00
28.82
B
C


ATOM
7902
O
LEU
B
420
43.356
27.280
−14.461
1.00
26.51
B
O


ATOM
7903
N
GLN
B
421
42.243
26.937
−12.539
1.00
28.46
B
N


ATOM
7904
CA
GLN
B
421
41.100
26.291
−13.193
1.00
28.02
B
C


ATOM
7905
CB
GLN
B
421
40.088
25.791
−12.166
1.00
35.06
B
C


ATOM
7906
CG
GLN
B
421
40.638
24.713
−11.299
1.00
37.28
B
C


ATOM
7907
CD
GLN
B
421
39.609
23.675
−10.893
1.00
39.96
B
C


ATOM
7908
OE1
GLN
B
421
39.746
22.499
−11.216
1.00
36.36
B
O


ATOM
7909
NE2
GLN
B
421
38.604
24.092
−10.162
1.00
28.86
B
N


ATOM
7910
C
GLN
B
421
40.398
27.211
−14.140
1.00
25.93
B
C


ATOM
7911
O
GLN
B
421
39.787
26.761
−15.107
1.00
28.33
B
O


ATOM
7912
N
LYS
B
422
40.458
28.511
−13.866
1.00
24.57
B
N


ATOM
7913
CA
LYS
B
422
39.885
29.512
−14.765
1.00
25.71
B
C


ATOM
7914
CB
LYS
B
422
39.296
30.684
−13.943
1.00
31.56
B
C


ATOM
7915
CG
LYS
B
422
38.012
30.279
−13.227
1.00
51.62
B
C


ATOM
7916
CD
LYS
B
422
37.660
31.126
−11.988
1.00
60.90
B
C


ATOM
7917
CE
LYS
B
422
36.364
30.571
−11.331
1.00
65.83
B
C


ATOM
7918
NZ
LYS
B
422
36.046
31.096
−9.972
1.00
54.30
B
N


ATOM
7919
C
LYS
B
422
40.920
30.032
−15.765
1.00
23.39
B
C


ATOM
7920
O
LYS
B
422
40.695
31.032
−16.443
1.00
28.46
B
O


ATOM
7921
N
TYR
B
423
42.063
29.372
−15.872
1.00
22.71
B
N


ATOM
7922
CA
TYR
B
423
43.074
29.773
−16.864
1.00
20.56
B
C


ATOM
7923
CB
TYR
B
423
42.574
29.603
−18.283
1.00
21.24
B
C


ATOM
7924
CG
TYR
B
423
42.196
28.181
−18.626
1.00
25.60
B
C


ATOM
7925
CD1
TYR
B
423
43.149
27.273
−19.024
1.00
23.47
B
C


ATOM
7926
CE1
TYR
B
423
42.792
25.962
−19.367
1.00
28.05
B
C


ATOM
7927
CZ
TYR
B
423
41.454
25.577
−19.260
1.00
32.51
B
C


ATOM
7928
OH
TYR
B
423
41.037
24.313
−19.566
1.00
34.46
B
O


ATOM
7929
CE2
TYR
B
423
40.495
26.487
−18.859
1.00
34.11
B
C


ATOM
7930
CD2
TYR
B
423
40.864
27.770
−18.566
1.00
27.96
B
C


ATOM
7931
C
TYR
B
423
43.532
31.214
−16.705
1.00
21.71
B
C


ATOM
7932
O
TYR
B
423
43.536
31.989
−17.650
1.00
20.05
B
O


ATOM
7933
N
HIS
B
424
43.991
31.518
−15.506
1.00
22.59
B
N


ATOM
7934
CA
HIS
B
424
44.562
32.815
−15.181
1.00
21.09
B
C


ATOM
7935
CB
HIS
B
424
45.067
32.740
−13.750
1.00
23.23
B
C


ATOM
7936
CG
HIS
B
424
45.673
34.023
−13.239
1.00
26.81
B
C


ATOM
7937
ND1
HIS
B
424
44.935
34.992
−12.625
1.00
31.97
B
N


ATOM
7938
CE1
HIS
B
424
45.748
36.011
−12.278
1.00
22.71
B
C


ATOM
7939
NE2
HIS
B
424
46.988
35.715
−12.678
1.00
31.93
B
N


ATOM
7940
CD2
HIS
B
424
46.970
34.489
−13.281
1.00
25.44
B
C


ATOM
7941
C
HIS
B
424
45.707
33.105
−16.133
1.00
21.91
B
C


ATOM
7942
O
HIS
B
424
46.430
32.196
−16.599
1.00
17.91
B
O


ATOM
7943
N
ASP
B
425
45.883
34.374
−16.429
1.00
17.02
B
N


ATOM
7944
CA
ASP
B
425
46.957
34.854
−17.305
1.00
22.29
B
C


ATOM
7945
CB
ASP
B
425
47.130
36.361
−17.128
1.00
31.01
B
C


ATOM
7946
CG
ASP
B
425
45.940
37.145
−17.611
1.00
35.91
B
C


ATOM
7947
OD1
ASP
B
425
45.264
36.690
−18.559
1.00
43.87
B
O


ATOM
7948
OD2
ASP
B
425
45.688
38.218
−17.020
1.00
43.05
B
O


ATOM
7949
C
ASP
B
425
48.338
34.264
−17.087
1.00
21.04
B
C


ATOM
7950
O
ASP
B
425
49.110
34.165
−18.025
1.00
17.11
B
O


ATOM
7951
N
THR
B
426
48.667
33.940
−15.844
1.00
20.31
B
N


ATOM
7952
CA
THR
B
426
49.951
33.336
−15.488
1.00
19.84
B
C


ATOM
7953
CB
THR
B
426
50.004
33.029
−13.970
1.00
23.34
B
C


ATOM
7954
OG1
THR
B
426
49.890
34.283
−13.256
1.00
27.25
B
O


ATOM
7955
CG2
THR
B
426
51.301
32.410
−13.590
1.00
24.23
B
C


ATOM
7956
C
THR
B
426
50.242
32.088
−16.290
1.00
20.14
B
C


ATOM
7957
O
THR
B
426
51.399
31.857
−16.665
1.00
18.00
B
O


ATOM
7958
N
ILE
B
427
49.194
31.295
−16.554
1.00
17.26
B
N


ATOM
7959
CA
ILE
B
427
49.339
30.057
−17.301
1.00
16.26
B
C


ATOM
7960
CB
ILE
B
427
48.749
28.814
−16.594
1.00
17.08
B
C


ATOM
7961
CG1
ILE
B
427
47.227
28.781
−16.579
1.00
19.40
B
C


ATOM
7962
CD1
ILE
B
427
46.679
27.474
−15.907
1.00
20.08
B
C


ATOM
7963
CG2
ILE
B
427
49.293
28.697
−15.175
1.00
18.97
B
C


ATOM
7964
C
ILE
B
427
48.829
30.182
−18.710
1.00
17.22
B
C


ATOM
7965
O
ILE
B
427
49.347
29.520
−19.604
1.00
20.85
B
O


ATOM
7966
N
SER
B
428
47.842
31.045
−18.962
1.00
20.43
B
N


ATOM
7967
CA
SER
B
428
47.309
31.113
−20.321
1.00
15.80
B
C


ATOM
7968
CB
SER
B
428
45.945
31.785
−20.348
1.00
21.10
B
C


ATOM
7969
OG
SER
B
428
46.037
33.132
−20.023
1.00
20.25
B
O


ATOM
7970
C
SER
B
428
48.290
31.806
−21.264
1.00
17.17
B
C


ATOM
7971
O
SER
B
428
48.473
31.356
−22.380
1.00
18.80
B
O


ATOM
7972
N
ARG
B
429
48.995
32.852
−20.835
1.00
16.72
B
N


ATOM
7973
CA
AARG
B
429
49.828
33.594
−21.788
0.50
15.43
B
C


ATOM
7974
CA
BARG
B
429
49.833
33.608
−21.780
0.50
16.11
B
C


ATOM
7975
CB
AARG
B
429
50.238
34.952
−21.233
0.50
18.21
B
C


ATOM
7976
CB
BARG
B
429
50.291
34.946
−21.195
0.50
20.44
B
C


ATOM
7977
CG
AARG
B
429
49.080
35.944
−21.200
0.50
18.18
B
C


ATOM
7978
CG
BARG
B
429
49.166
35.882
−20.723
0.50
22.04
B
C


ATOM
7979
CD
AARG
B
429
49.539
37.351
−20.892
0.50
21.00
B
C


ATOM
7980
CD
BARG
B
429
48.398
36.559
−21.847
0.50
26.45
B
C


ATOM
7981
NE
AARG
B
429
48.441
38.247
−20.522
0.50
22.76
B
N


ATOM
7982
NE
BARG
B
429
47.476
37.580
−21.324
0.50
28.17
B
N


ATOM
7983
CZ
AARG
B
429
48.322
38.845
−19.342
0.50
20.32
B
C


ATOM
7984
CZ
BARG
B
429
46.530
38.181
−22.036
0.50
25.21
B
C


ATOM
7985
NH1
AARG
B
429
49.219
38.662
−18.389
0.50
27.90
B
N


ATOM
7986
NH1
BARG
B
429
46.361
37.881
−23.304
0.50
22.10
B
N


ATOM
7987
NH2
AARG
B
429
47.315
39.640
−19.133
0.50
22.88
B
N


ATOM
7988
NH2
BARG
B
429
45.768
39.099
−21.472
0.50
32.07
B
N


ATOM
7989
C
ARG
B
429
51.015
32.767
−22.274
1.00
17.61
B
C


ATOM
7990
O
ARG
B
429
51.282
32.725
−23.457
1.00
14.57
B
O


ATOM
7991
N
PRO
B
430
51.712
32.054
−21.363
1.00
14.73
B
N


ATOM
7992
CA
PRO
B
430
52.757
31.188
−21.898
1.00
15.75
B
C


ATOM
7993
CB
PRO
B
430
53.397
30.550
−20.652
1.00
17.07
B
C


ATOM
7994
CG
PRO
B
430
52.846
31.249
−19.511
1.00
20.36
B
C


ATOM
7995
CD
PRO
B
430
51.730
32.117
−19.892
1.00
16.63
B
C


ATOM
7996
C
PRO
B
430
52.243
30.083
−22.815
1.00
14.70
B
C


ATOM
7997
O
PRO
B
430
52.975
29.648
−23.726
1.00
15.79
B
O


ATOM
7998
N
SER
B
431
51.012
29.633
−22.582
1.00
14.43
B
N


ATOM
7999
CA
SER
B
431
50.356
28.622
−23.449
1.00
14.37
B
C


ATOM
8000
CB
SER
B
431
49.083
28.084
−22.762
1.00
13.13
B
C


ATOM
8001
OG
SER
B
431
49.502
27.415
−21.527
1.00
18.20
B
O


ATOM
8002
C
SER
B
431
50.054
29.198
−24.822
1.00
17.17
B
C


ATOM
8003
O
SER
B
431
50.140
28.494
−25.833
1.00
16.81
B
O


ATOM
8004
N
HIS
B
432
49.737
30.499
−24.859
1.00
14.64
B
N


ATOM
8005
CA
HIS
B
432
49.577
31.190
−26.130
1.00
15.68
B
C


ATOM
8006
CB
HIS
B
432
49.223
32.656
−25.968
1.00
14.64
B
C


ATOM
8007
CG
HIS
B
432
47.877
32.917
−25.321
1.00
18.93
B
C


ATOM
8008
ND1
HIS
B
432
47.439
34.166
−25.059
1.00
22.86
B
N


ATOM
8009
CE1
HIS
B
432
46.212
34.100
−24.472
1.00
25.01
B
C


ATOM
8010
NE2
HIS
B
432
45.881
32.819
−24.357
1.00
19.96
B
N


ATOM
8011
CD2
HIS
B
432
46.910
32.063
−24.846
1.00
17.39
B
C


ATOM
8012
C
HIS
B
432
50.825
31.072
−26.925
1.00
14.11
B
C


ATOM
8013
O
HIS
B
432
50.805
30.707
−28.110
1.00
13.66
B
O


ATOM
8014
N
ILE
B
433
51.940
31.399
−26.286
1.00
15.75
B
N


ATOM
8015
CA
ILE
B
433
53.232
31.332
−26.955
1.00
14.75
B
C


ATOM
8016
CB
ILE
B
433
54.329
32.001
−26.067
1.00
15.98
B
C


ATOM
8017
CG1
ILE
B
433
54.013
33.539
−26.056
1.00
18.75
B
C


ATOM
8018
CD1
ILE
B
433
54.706
34.306
−25.048
1.00
24.29
B
C


ATOM
8019
CG2
ILE
B
433
55.727
31.742
−26.691
1.00
15.81
B
C


ATOM
8020
C
ILE
B
433
53.609
29.929
−27.451
1.00
14.07
B
C


ATOM
8021
O
ILE
B
433
54.129
29.767
−28.587
1.00
15.08
B
O


ATOM
8022
N
PHE
B
434
53.366
28.931
−26.611
1.00
14.80
B
N


ATOM
8023
CA
PHE
B
434
53.631
27.514
−26.921
1.00
14.96
B
C


ATOM
8024
CB
PHE
B
434
53.164
26.715
−25.697
1.00
15.79
B
C


ATOM
8025
CG
PHE
B
434
53.298
25.222
−25.792
1.00
16.92
B
C


ATOM
8026
CD1
PHE
B
434
54.039
24.579
−26.793
1.00
19.94
B
C


ATOM
8027
CE1
PHE
B
434
54.158
23.160
−26.796
1.00
19.87
B
C


ATOM
8028
CZ
PHE
B
434
53.576
22.407
−25.787
1.00
22.06
B
C


ATOM
8029
CE2
PHE
B
434
52.850
23.042
−24.785
1.00
19.60
B
C


ATOM
8030
CD2
PHE
B
434
52.730
24.453
−24.791
1.00
17.79
B
C


ATOM
8031
C
PHE
B
434
52.907
27.120
−28.228
1.00
14.03
B
C


ATOM
8032
O
PHE
B
434
53.528
26.604
−29.165
1.00
16.19
B
O


ATOM
8033
N
ARG
B
435
51.622
27.445
−28.307
1.00
14.78
B
N


ATOM
8034
CA
ARG
B
435
50.819
27.116
−29.488
1.00
12.98
B
C


ATOM
8035
CB
ARG
B
435
49.327
27.369
−29.195
1.00
14.24
B
C


ATOM
8036
CG
ARG
B
435
48.375
27.168
−30.367
1.00
13.52
B
C


ATOM
8037
CD
ARG
B
435
48.591
25.871
−31.145
1.00
14.92
B
C


ATOM
8038
NE
ARG
B
435
48.185
24.704
−30.372
1.00
16.56
B
N


ATOM
8039
CZ
ARG
B
435
48.453
23.449
−30.711
1.00
19.64
B
C


ATOM
8040
NH1
ARG
B
435
49.195
23.185
−31.782
1.00
19.87
B
N


ATOM
8041
NH2
ARG
B
435
47.963
22.460
−29.973
1.00
18.55
B
N


ATOM
8042
C
ARG
B
435
51.291
27.886
−30.683
1.00
14.00
B
C


ATOM
8043
O
ARG
B
435
51.409
27.317
−31.759
1.00
14.38
B
O


ATOM
8044
N
LEU
B
436
51.581
29.188
−30.529
1.00
14.56
B
N


ATOM
8045
CA
LEU
B
436
51.968
29.977
−31.682
1.00
13.42
B
C


ATOM
8046
CB
LEU
B
436
51.924
31.485
−31.419
1.00
14.94
B
C


ATOM
8047
CG
LEU
B
436
50.544
32.075
−31.099
1.00
14.41
B
C


ATOM
8048
CD1
LEU
B
436
50.640
33.516
−30.626
1.00
14.92
B
C


ATOM
8049
CD2
LEU
B
436
49.602
31.922
−32.295
1.00
13.74
B
C


ATOM
8050
C
LEU
B
436
53.344
29.555
−32.203
1.00
15.66
B
C


ATOM
8051
O
LEU
B
436
53.532
29.483
−33.418
1.00
19.19
B
O


ATOM
8052
N
CYS
B
437
54.293
29.272
−31.301
1.00
16.96
B
N


ATOM
8053
CA
CYS
B
437
55.603
28.766
−31.734
1.00
16.93
B
C


ATOM
8054
CB
CYS
B
437
56.536
28.577
−30.559
1.00
17.77
B
C


ATOM
8055
SG
CYS
B
437
57.150
30.113
−29.871
1.00
22.06
B
S


ATOM
8056
C
CYS
B
437
55.503
27.451
−32.498
1.00
14.77
B
C


ATOM
8057
O
CYS
B
437
56.099
27.296
−33.550
1.00
18.12
B
O


ATOM
8058
N
ASN
B
438
54.727
26.520
−31.966
1.00
17.15
B
N


ATOM
8059
CA
ASN
B
438
54.519
25.218
−32.568
1.00
16.89
B
C


ATOM
8060
CB
ASN
B
438
53.649
24.381
−31.646
1.00
16.59
B
C


ATOM
8061
CG
ASN
B
438
53.345
22.972
−32.214
1.00
24.82
B
C


ATOM
8062
OD1
ASN
B
438
53.826
22.576
−33.265
1.00
33.49
B
O


ATOM
8063
ND2
ASN
B
438
52.657
22.205
−31.447
1.00
22.35
B
N


ATOM
8064
C
ASN
B
438
53.900
25.390
−33.951
1.00
20.33
B
C


ATOM
8065
O
ASN
B
438
54.399
24.838
−34.952
1.00
19.00
B
O


ATOM
8066
N
ASP
B
439
52.857
26.214
−34.033
1.00
15.44
B
N


ATOM
8067
CA
ASP
B
439
52.159
26.398
−35.307
1.00
15.06
B
C


ATOM
8068
CB
ASP
B
439
50.765
27.022
−35.120
1.00
16.90
B
C


ATOM
8069
CG
ASP
B
439
49.744
26.019
−34.541
1.00
19.98
B
C


ATOM
8070
OD1
ASP
B
439
50.174
24.934
−34.065
1.00
17.50
B
O


ATOM
8071
OD2
ASP
B
439
48.513
26.315
−34.523
1.00
18.13
B
O


ATOM
8072
C
ASP
B
439
52.989
27.155
−36.326
1.00
16.19
B
C


ATOM
8073
O
ASP
B
439
52.888
26.870
−37.516
1.00
17.47
B
O


ATOM
8074
N
LEU
B
440
53.820
28.094
−35.882
1.00
15.61
B
N


ATOM
8075
CA
LEU
B
440
54.676
28.794
−36.811
1.00
18.75
B
C


ATOM
8076
CB
LEU
B
440
55.471
29.906
−36.122
1.00
21.99
B
C


ATOM
8077
CG
LEU
B
440
54.750
31.220
−35.858
1.00
20.97
B
C


ATOM
8078
CD1
LEU
B
440
55.562
31.999
−34.845
1.00
19.22
B
C


ATOM
8079
CD2
LEU
B
440
54.559
32.027
−37.128
1.00
17.97
B
C


ATOM
8080
C
LEU
B
440
55.688
27.811
−37.451
1.00
19.73
B
C


ATOM
8081
O
LEU
B
440
56.004
27.961
−38.621
1.00
18.40
B
O


ATOM
8082
N
ALA
B
441
56.182
26.845
−36.674
1.00
20.07
B
N


ATOM
8083
CA
ALA
B
441
57.214
25.894
−37.157
1.00
23.25
B
C


ATOM
8084
CB
ALA
B
441
57.813
25.077
−35.983
1.00
19.15
B
C


ATOM
8085
C
ALA
B
441
56.640
24.971
−38.228
1.00
25.11
B
C


ATOM
8086
O
ALA
B
441
57.327
24.606
−39.161
1.00
23.98
B
O


ATOM
8087
N
SER
B
442
55.359
24.639
−38.137
1.00
23.43
B
N


ATOM
8088
CA
SER
B
442
54.767
23.738
−39.119
1.00
27.50
B
C


ATOM
8089
CB
SER
B
442
53.991
22.640
−38.385
1.00
26.04
B
C


ATOM
8090
OG
SER
B
442
52.935
23.180
−37.640
1.00
34.78
B
O


ATOM
8091
C
SER
B
442
53.889
24.429
−40.180
1.00
24.60
B
C


ATOM
8092
O
SER
B
442
53.387
23.763
−41.103
1.00
23.82
B
O


ATOM
8093
N
ALA
B
443
53.741
25.755
−40.088
1.00
26.78
B
N


ATOM
8094
CA
ALA
B
443
52.788
26.481
−40.938
1.00
25.34
B
C


ATOM
8095
CB
ALA
B
443
52.813
27.978
−40.637
1.00
22.95
B
C


ATOM
8096
C
ALA
B
443
53.062
26.247
−42.431
1.00
28.89
B
C


ATOM
8097
O
ALA
B
443
52.157
25.916
−43.185
1.00
22.42
B
O


ATOM
8098
N
SER
B
444
54.300
26.458
−42.868
1.00
27.66
B
N


ATOM
8099
CA
SER
B
444
54.589
26.373
−44.312
1.00
33.85
B
C


ATOM
8100
CB
SER
B
444
56.058
26.640
−44.598
1.00
36.92
B
C


ATOM
8101
OG
SER
B
444
56.346
28.011
−44.385
1.00
47.06
B
O


ATOM
8102
C
SER
B
444
54.180
25.031
−44.901
1.00
30.05
B
C


ATOM
8103
O
SER
B
444
53.466
24.980
−45.900
1.00
30.05
B
O


ATOM
8104
N
ALA
B
445
54.602
23.945
−44.263
1.00
29.24
B
N


ATOM
8105
CA
ALA
B
445
54.315
22.616
−44.793
1.00
35.03
B
C


ATOM
8106
CB
ALA
B
445
55.129
21.522
−44.045
1.00
32.05
B
C


ATOM
8107
C
ALA
B
445
52.807
22.332
−44.755
1.00
37.09
B
C


ATOM
8108
O
ALA
B
445
52.257
21.787
−45.704
1.00
33.84
B
O


ATOM
8109
N
GLU
B
446
52.124
22.734
−43.685
1.00
30.46
B
N


ATOM
8110
CA
GLU
B
446
50.697
22.475
−43.579
1.00
28.45
B
C


ATOM
8111
CB
GLU
B
446
50.218
22.656
−42.139
1.00
37.91
B
C


ATOM
8112
CG
GLU
B
446
50.638
21.476
−41.256
1.00
42.02
B
C


ATOM
8113
CD
GLU
B
446
50.457
21.692
−39.740
1.00
42.41
B
C


ATOM
8114
OE1
GLU
B
446
49.785
22.643
−39.310
1.00
45.73
B
O


ATOM
8115
OE2
GLU
B
446
51.005
20.887
−38.958
1.00
43.12
B
O


ATOM
8116
C
GLU
B
446
49.890
23.315
−44.573
1.00
33.90
B
C


ATOM
8117
O
GLU
B
446
48.937
22.819
−45.169
1.00
31.66
B
O


ATOM
8118
N
ILE
B
447
50.293
24.568
−44.794
1.00
27.20
B
N


ATOM
8119
CA
ILE
B
447
49.579
25.424
−45.737
1.00
27.83
B
C


ATOM
8120
CB
ILE
B
447
50.055
26.895
−45.659
1.00
28.75
B
C


ATOM
8121
CG1
ILE
B
447
49.600
27.490
−44.320
1.00
27.61
B
C


ATOM
8122
CD1
ILE
B
447
50.393
28.640
−43.844
1.00
26.97
B
C


ATOM
8123
CG2
ILE
B
447
49.500
27.723
−46.845
1.00
28.72
B
C


ATOM
8124
C
ILE
B
447
49.705
24.847
−47.155
1.00
36.77
B
C


ATOM
8125
O
ILE
B
447
48.703
24.713
−47.863
1.00
38.04
B
O


ATOM
8126
N
ALA
B
448
50.919
24.442
−47.526
1.00
31.66
B
N


ATOM
8127
CA
ALA
B
448
51.194
23.890
−48.854
1.00
36.12
B
C


ATOM
8128
CB
ALA
B
448
52.707
23.879
−49.127
1.00
26.57
B
C


ATOM
8129
C
ALA
B
448
50.592
22.493
−49.052
1.00
39.86
B
C


ATOM
8130
O
ALA
B
448
50.465
22.039
−50.180
1.00
48.95
B
O


ATOM
8131
N
ARG
B
449
50.238
21.817
−47.960
1.00
40.90
B
N


ATOM
8132
CA
ARG
B
449
49.462
20.574
−48.014
1.00
43.27
B
C


ATOM
8133
CB
ARG
B
449
49.719
19.719
−46.767
1.00
45.19
B
C


ATOM
8134
CG
ARG
B
449
50.845
18.718
−46.901
1.00
46.47
B
C


ATOM
8135
CD
ARG
B
449
51.014
17.908
−45.619
1.00
54.26
B
C


ATOM
8136
NE
ARG
B
449
52.120
18.386
−44.785
1.00
65.38
B
N


ATOM
8137
CZ
ARG
B
449
52.152
18.377
−43.446
1.00
64.14
B
C


ATOM
8138
NH1
ARG
B
449
51.126
17.936
−42.726
1.00
67.70
B
N


ATOM
8139
NH2
ARG
B
449
53.228
18.834
−42.814
1.00
67.87
B
N


ATOM
8140
C
ARG
B
449
47.954
20.814
−48.115
1.00
44.23
B
C


ATOM
8141
O
ARG
B
449
47.186
19.862
−48.221
1.00
47.36
B
O


ATOM
8142
N
GLY
B
450
47.519
22.065
−48.044
1.00
42.67
B
N


ATOM
8143
CA
GLY
B
450
46.083
22.375
−48.002
1.00
40.45
B
C


ATOM
8144
C
GLY
B
450
45.411
22.196
−46.641
1.00
45.63
B
C


ATOM
8145
O
GLY
B
450
44.193
22.253
−46.544
1.00
39.65
B
O


ATOM
8146
N
GLU
B
451
46.191
21.995
−45.578
1.00
46.83
B
N


ATOM
8147
CA
GLU
B
451
45.620
21.874
−44.236
1.00
43.73
B
C


ATOM
8148
CB
GLU
B
451
46.578
21.142
−43.299
1.00
57.06
B
C


ATOM
8149
CG
GLU
B
451
46.765
19.683
−43.695
1.00
61.57
B
C


ATOM
8150
CD
GLU
B
451
47.876
19.009
−42.931
1.00
64.10
B
C


ATOM
8151
OE1
GLU
B
451
47.993
19.254
−41.714
1.00
65.34
B
O


ATOM
8152
OE2
GLU
B
451
48.627
18.227
−43.550
1.00
61.42
B
O


ATOM
8153
C
GLU
B
451
45.258
23.234
−43.663
1.00
38.50
B
C


ATOM
8154
O
GLU
B
451
45.840
24.259
−44.026
1.00
45.27
B
O


ATOM
8155
N
THR
B
452
44.258
23.246
−42.793
1.00
31.58
B
N


ATOM
8156
CA
THR
B
452
43.761
24.488
−42.242
1.00
31.96
B
C


ATOM
8157
CB
THR
B
452
42.312
24.772
−42.689
1.00
34.79
B
C


ATOM
8158
OG1
THR
B
452
41.462
23.731
−42.199
1.00
34.40
B
O


ATOM
8159
CG2
THR
B
452
42.202
24.905
−44.225
1.00
30.65
B
C


ATOM
8160
C
THR
B
452
43.828
24.477
−40.717
1.00
28.29
B
C


ATOM
8161
O
THR
B
452
43.566
25.508
−40.090
1.00
28.98
B
O


ATOM
8162
N
ALA
B
453
44.223
23.337
−40.135
1.00
29.72
B
N


ATOM
8163
CA
ALA
B
453
44.308
23.177
−38.684
1.00
27.93
B
C


ATOM
8164
CB
ALA
B
453
44.159
21.712
−38.293
1.00
26.92
B
C


ATOM
8165
C
ALA
B
453
45.650
23.716
−38.179
1.00
31.25
B
C


ATOM
8166
O
ALA
B
453
46.482
22.957
−37.723
1.00
25.19
B
O


ATOM
8167
N
ASN
B
454
45.828
25.023
−38.276
1.00
23.93
B
N


ATOM
8168
CA
ASN
B
454
47.045
25.699
−37.889
1.00
21.21
B
C


ATOM
8169
CB
ASN
B
454
48.083
25.582
−39.014
1.00
21.74
B
C


ATOM
8170
CG
ASN
B
454
49.394
26.258
−38.685
1.00
21.29
B
C


ATOM
8171
OD1
ASN
B
454
49.513
27.465
−38.794
1.00
22.92
B
O


ATOM
8172
ND2
ASN
B
454
50.413
25.473
−38.365
1.00
18.97
B
N


ATOM
8173
C
ASN
B
454
46.705
27.151
−37.638
1.00
18.41
B
C


ATOM
8174
O
ASN
B
454
46.035
27.771
−38.450
1.00
16.21
B
O


ATOM
8175
N
SER
B
455
47.221
27.711
−36.543
1.00
19.01
B
N


ATOM
8176
CA
SER
B
455
47.002
29.113
−36.202
1.00
16.68
B
C


ATOM
8177
CB
SER
B
455
47.781
29.488
−34.921
1.00
20.45
B
C


ATOM
8178
OG
SER
B
455
47.301
28.678
−33.853
1.00
19.49
B
O


ATOM
8179
C
SER
B
455
47.319
30.099
−37.316
1.00
19.30
B
C


ATOM
8180
O
SER
B
455
46.540
31.034
−37.548
1.00
15.67
B
O


ATOM
8181
N
VAL
B
456
48.432
29.915
−38.024
1.00
15.11
B
N


ATOM
8182
CA
VAL
B
456
48.828
30.843
−39.062
1.00
15.74
B
C


ATOM
8183
CB
VAL
B
456
50.299
30.596
−39.541
1.00
19.50
B
C


ATOM
8184
CG1
VAL
B
456
50.688
31.606
−40.590
1.00
20.50
B
C


ATOM
8185
CG2
VAL
B
456
51.251
30.648
−38.368
1.00
15.54
B
C


ATOM
8186
C
VAL
B
456
47.852
30.763
−40.237
1.00
16.05
B
C


ATOM
8187
O
VAL
B
456
47.410
31.785
−40.806
1.00
14.34
B
O


ATOM
8188
N
SER
B
457
47.490
29.544
−40.583
1.00
15.10
B
N


ATOM
8189
CA
SER
B
457
46.511
29.323
−41.616
1.00
18.41
B
C


ATOM
8190
CB
SER
B
457
46.293
27.846
−41.869
1.00
20.92
B
C


ATOM
8191
OG
SER
B
457
45.167
27.772
−42.724
1.00
26.24
B
O


ATOM
8192
C
SER
B
457
45.158
29.949
−41.284
1.00
17.65
B
C


ATOM
8193
O
SER
B
457
44.531
30.564
−42.146
1.00
14.43
B
O


ATOM
8194
N
CYS
B
458
44.704
29.804
−40.043
1.00
15.66
B
N


ATOM
8195
CA
CYS
B
458
43.444
30.453
−39.640
1.00
18.01
B
C


ATOM
8196
CB
CYS
B
458
43.054
30.160
−38.192
1.00
25.62
B
C


ATOM
8197
SG
CYS
B
458
42.256
28.699
−38.041
1.00
35.73
B
S


ATOM
8198
C
CYS
B
458
43.517
31.929
−39.741
1.00
12.07
B
C


ATOM
8199
O
CYS
B
458
42.566
32.557
−40.176
1.00
16.64
B
O


ATOM
8200
N
TYR
B
459
44.629
32.503
−39.306
1.00
14.40
B
N


ATOM
8201
CA
TYR
B
459
44.794
33.931
−39.350
1.00
14.44
B
C


ATOM
8202
CB
TYR
B
459
46.096
34.315
−38.667
1.00
17.94
B
C


ATOM
8203
CG
TYR
B
459
46.120
35.710
−38.123
1.00
18.08
B
C


ATOM
8204
CD1
TYR
B
459
46.388
36.781
−38.958
1.00
23.82
B
C


ATOM
8205
CE1
TYR
B
459
46.419
38.077
−38.479
1.00
25.57
B
C


ATOM
8206
CZ
TYR
B
459
46.213
38.329
−37.149
1.00
25.18
B
C


ATOM
8207
OH
TYR
B
459
46.262
39.634
−36.748
1.00
37.10
B
O


ATOM
8208
CE2
TYR
B
459
45.977
37.300
−36.272
1.00
24.97
B
C


ATOM
8209
CD2
TYR
B
459
45.932
35.967
−36.772
1.00
25.54
B
C


ATOM
8210
C
TYR
B
459
44.740
34.421
−40.797
1.00
16.51
B
C


ATOM
8211
O
TYR
B
459
44.078
35.445
−41.132
1.00
14.82
B
O


ATOM
8212
N
MET
B
460
45.433
33.718
−41.686
1.00
13.76
B
N


ATOM
8213
CA
MET
B
460
45.348
34.057
−43.117
1.00
14.07
B
C


ATOM
8214
CB
MET
B
460
46.056
33.032
−44.002
1.00
15.60
B
C


ATOM
8215
CG
MET
B
460
47.540
33.067
−43.937
1.00
21.56
B
C


ATOM
8216
SD
MET
B
460
48.225
31.608
−44.774
1.00
24.76
B
S


ATOM
8217
CE
MET
B
460
47.831
31.926
−46.477
1.00
15.99
B
C


ATOM
8218
C
MET
B
460
43.917
34.084
−43.642
1.00
14.98
B
C


ATOM
8219
O
MET
B
460
43.527
34.991
−44.393
1.00
15.96
B
O


ATOM
8220
N
ARG
B
461
43.162
33.048
−43.329
1.00
13.68
B
N


ATOM
8221
CA
ARG
B
461
41.796
32.938
−43.848
1.00
16.87
B
C


ATOM
8222
CB
ARG
B
461
41.283
31.518
−43.727
1.00
23.77
B
C


ATOM
8223
CG
ARG
B
461
41.747
30.676
−44.914
1.00
32.85
B
C


ATOM
8224
CD
ARG
B
461
41.446
29.197
−44.770
1.00
34.57
B
C


ATOM
8225
NE
ARG
B
461
41.992
28.629
−43.532
1.00
34.16
B
N


ATOM
8226
CZ
ARG
B
461
41.289
28.392
−42.420
1.00
44.41
B
C


ATOM
8227
NH1
ARG
B
461
39.972
28.674
−42.354
1.00
33.15
B
N


ATOM
8228
NH2
ARG
B
461
41.917
27.875
−41.352
1.00
33.81
B
N


ATOM
8229
C
ARG
B
461
40.840
33.891
−43.167
1.00
13.78
B
C


ATOM
8230
O
ARG
B
461
39.967
34.412
−43.785
1.00
14.11
B
O


ATOM
8231
N
THR
B
462
41.018
34.106
−41.875
1.00
13.65
B
N


ATOM
8232
CA
THR
B
462
40.207
35.071
−41.150
1.00
12.52
B
C


ATOM
8233
CB
THR
B
462
40.442
34.981
−39.640
1.00
11.17
B
C


ATOM
8234
OG1
THR
B
462
39.831
33.778
−39.180
1.00
10.66
B
O


ATOM
8235
CG2
THR
B
462
39.844
36.207
−38.911
1.00
10.76
B
C


ATOM
8236
C
THR
B
462
40.396
36.491
−41.666
1.00
14.81
B
C


ATOM
8237
O
THR
B
462
39.421
37.173
−41.877
1.00
13.55
B
O


ATOM
8238
N
LYS
B
463
41.631
36.921
−41.872
1.00
16.92
B
N


ATOM
8239
CA
ALYS
B
463
41.919
38.298
−42.288
0.50
16.81
B
C


ATOM
8240
CA
BLYS
B
463
41.908
38.302
−42.277
0.50
16.46
B
C


ATOM
8241
CB
ALYS
B
463
43.207
38.775
−41.647
0.50
17.82
B
C


ATOM
8242
CB
BLYS
B
463
43.179
38.803
−41.607
0.50
16.89
B
C


ATOM
8243
CG
ALYS
B
463
43.039
39.156
−40.201
0.50
20.60
B
C


ATOM
8244
CG
BLYS
B
463
43.217
38.634
−40.073
0.50
18.51
B
C


ATOM
8245
CD
ALYS
B
463
42.486
40.556
−40.116
0.50
20.99
B
C


ATOM
8246
CD
BLYS
B
463
42.193
39.525
−39.394
0.50
19.15
B
C


ATOM
8247
CE
ALYS
B
463
42.523
41.081
−38.718
0.50
19.52
B
C


ATOM
8248
CE
BLYS
B
463
42.692
40.035
−38.046
0.50
21.93
B
C


ATOM
8249
NZ
ALYS
B
463
41.763
42.322
−38.646
0.50
18.07
B
N


ATOM
8250
NZ
BLYS
B
463
42.390
39.101
−36.923
0.50
22.18
B
N


ATOM
8251
C
LYS
B
463
42.015
38.480
−43.794
1.00
15.05
B
C


ATOM
8252
O
LYS
B
463
42.054
39.591
−44.284
1.00
18.45
B
O


ATOM
8253
N
GLY
B
464
42.040
37.393
−44.535
1.00
15.19
B
N


ATOM
8254
CA
GLY
B
464
42.135
37.464
−45.988
1.00
14.92
B
C


ATOM
8255
C
GLY
B
464
43.506
37.936
−46.454
1.00
15.23
B
C


ATOM
8256
O
GLY
B
464
43.601
38.672
−47.409
1.00
16.94
B
O


ATOM
8257
N
ILE
B
465
44.568
37.493
−45.787
1.00
16.53
B
N


ATOM
8258
CA
ILE
B
465
45.947
37.941
−46.099
1.00
15.71
B
C


ATOM
8259
CB
ILE
B
465
46.571
38.763
−44.951
1.00
14.86
B
C


ATOM
8260
CG1
ILE
B
465
46.690
37.925
−43.667
1.00
15.16
B
C


ATOM
8261
CD1
ILE
B
465
47.295
38.642
−42.526
1.00
17.97
B
C


ATOM
8262
CG2
ILE
B
465
45.772
40.069
−44.760
1.00
15.44
B
C


ATOM
8263
C
ILE
B
465
46.884
36.782
−46.442
1.00
16.44
B
C


ATOM
8264
O
ILE
B
465
46.605
35.603
−46.158
1.00
17.69
B
O


ATOM
8265
N
SER
B
466
48.032
37.143
−46.998
1.00
20.92
B
N


ATOM
8266
CA
SER
B
466
49.070
36.187
−47.379
1.00
20.24
B
C


ATOM
8267
CB
SER
B
466
50.179
36.913
−48.133
1.00
22.24
B
C


ATOM
8268
OG
SER
B
466
50.843
37.794
−47.243
1.00
20.55
B
O


ATOM
8269
C
SER
B
466
49.711
35.550
−46.160
1.00
19.68
B
C


ATOM
8270
O
SER
B
466
49.690
36.113
−45.036
1.00
17.72
B
O


ATOM
8271
N
GLU
B
467
50.332
34.400
−46.404
1.00
19.75
B
N


ATOM
8272
CA
GLU
B
467
51.117
33.716
−45.403
1.00
24.19
B
C


ATOM
8273
CB
GLU
B
467
51.714
32.423
−45.969
1.00
26.22
B
C


ATOM
8274
CG
GLU
B
467
52.518
31.664
−44.911
1.00
29.10
B
C


ATOM
8275
CD
GLU
B
467
53.050
30.306
−45.365
1.00
36.05
B
C


ATOM
8276
OE1
GLU
B
467
52.638
29.809
−46.434
1.00
35.86
B
O


ATOM
8277
OE2
GLU
B
467
53.887
29.733
−44.622
1.00
33.33
B
O


ATOM
8278
C
GLU
B
467
52.203
34.601
−44.777
1.00
22.33
B
C


ATOM
8279
O
GLU
B
467
52.410
34.537
−43.575
1.00
23.79
B
O


ATOM
8280
N
GLU
B
468
52.845
35.464
−45.564
1.00
22.39
B
N


ATOM
8281
CA
GLU
B
468
53.918
36.330
−45.038
1.00
27.65
B
C


ATOM
8282
CB
GLU
B
468
54.631
37.123
−46.147
1.00
33.61
B
C


ATOM
8283
CG
GLU
B
468
55.397
36.289
−47.150
1.00
50.09
B
C


ATOM
8284
CD
GLU
B
468
54.509
35.368
−48.016
1.00
66.40
B
C


ATOM
8285
OE1
GLU
B
468
53.401
35.772
−48.447
1.00
49.17
B
O


ATOM
8286
OE2
GLU
B
468
54.925
34.216
−48.262
1.00
85.08
B
O


ATOM
8287
C
GLU
B
468
53.358
37.328
−44.049
1.00
21.77
B
C


ATOM
8288
O
GLU
B
468
53.929
37.539
−42.995
1.00
20.88
B
O


ATOM
8289
N
LEU
B
469
52.230
37.947
−44.398
1.00
19.47
B
N


ATOM
8290
CA
LEU
B
469
51.601
38.915
−43.508
1.00
19.05
B
C


ATOM
8291
CB
LEU
B
469
50.547
39.747
−44.229
1.00
23.07
B
C


ATOM
8292
CG
LEU
B
469
51.089
40.827
−45.164
1.00
29.43
B
C


ATOM
8293
CD1
LEU
B
469
50.033
41.218
−46.221
1.00
24.90
B
C


ATOM
8294
CD2
LEU
B
469
51.578
42.023
−44.341
1.00
33.35
B
C


ATOM
8295
C
LEU
B
469
50.998
38.235
−42.269
1.00
16.57
B
C


ATOM
8296
O
LEU
B
469
51.095
38.760
−41.196
1.00
16.59
B
O


ATOM
8297
N
ALA
B
470
50.414
37.059
−42.417
1.00
16.46
B
N


ATOM
8298
CA
ALA
B
470
49.867
36.352
−41.268
1.00
17.72
B
C


ATOM
8299
CB
ALA
B
470
49.099
35.112
−41.714
1.00
14.43
B
C


ATOM
8300
C
ALA
B
470
51.005
35.984
−40.307
1.00
19.08
B
C


ATOM
8301
O
ALA
B
470
50.889
36.125
−39.090
1.00
16.48
B
O


ATOM
8302
N
THR
B
471
52.123
35.534
−40.868
1.00
17.61
B
N


ATOM
8303
CA
THR
B
471
53.299
35.178
−40.068
1.00
16.26
B
C


ATOM
8304
CB
THR
B
471
54.441
34.621
−40.963
1.00
17.28
B
C


ATOM
8305
OG1
THR
B
471
53.916
33.497
−41.675
1.00
18.87
B
O


ATOM
8306
CG2
THR
B
471
55.661
34.220
−40.120
1.00
17.17
B
C


ATOM
8307
C
THR
B
471
53.800
36.342
−39.244
1.00
17.20
B
C


ATOM
8308
O
THR
B
471
54.001
36.218
−38.024
1.00
14.80
B
O


ATOM
8309
N
GLU
B
472
53.991
37.473
−39.904
1.00
18.72
B
N


ATOM
8310
CA
GLU
B
472
54.323
38.729
−39.230
1.00
23.12
B
C


ATOM
8311
CB
GLU
B
472
54.502
39.824
−40.305
1.00
26.44
B
C


ATOM
8312
CG
GLU
B
472
54.573
41.278
−39.863
1.00
37.27
B
C


ATOM
8313
CD
GLU
B
472
54.716
42.236
−41.077
1.00
41.81
B
C


ATOM
8314
OE1
GLU
B
472
55.849
42.428
−41.548
1.00
46.89
B
O


ATOM
8315
OE2
GLU
B
472
53.699
42.774
−41.573
1.00
42.94
B
O


ATOM
8316
C
GLU
B
472
53.313
39.124
−38.101
1.00
22.60
B
C


ATOM
8317
O
GLU
B
472
53.735
39.469
−36.977
1.00
19.81
B
O


ATOM
8318
N
SER
B
473
52.007
39.018
−38.360
1.00
19.86
B
N


ATOM
8319
CA
SER
B
473
51.014
39.293
−37.299
1.00
20.06
B
C


ATOM
8320
CB
SER
B
473
49.572
39.208
−37.815
1.00
22.53
B
C


ATOM
8321
OG
SER
B
473
49.304
40.264
−38.673
1.00
29.40
B
O


ATOM
8322
C
SER
B
473
51.152
38.316
−36.143
1.00
15.27
B
C


ATOM
8323
O
SER
B
473
51.038
38.697
−35.001
1.00
17.14
B
O


ATOM
8324
N
VAL
B
474
51.398
37.055
−36.438
1.00
15.88
B
N


ATOM
8325
CA
VAL
B
474
51.594
36.093
−35.377
1.00
15.12
B
C


ATOM
8326
CB
VAL
B
474
51.608
34.665
−35.926
1.00
15.21
B
C


ATOM
8327
CG1
VAL
B
474
52.067
33.652
−34.800
1.00
13.14
B
C


ATOM
8328
CG2
VAL
B
474
50.228
34.299
−36.427
1.00
15.92
B
C


ATOM
8329
C
VAL
B
474
52.846
36.414
−34.522
1.00
15.98
B
C


ATOM
8330
O
VAL
B
474
52.795
36.410
−33.272
1.00
16.50
B
O


ATOM
8331
N
MET
B
475
53.934
36.796
−35.165
1.00
19.87
B
N


ATOM
8332
CA
MET
B
475
55.127
37.241
−34.424
1.00
18.38
B
C


ATOM
8333
CB
MET
B
475
56.282
37.591
−35.376
1.00
21.15
B
C


ATOM
8334
CG
MET
B
475
56.744
36.508
−36.344
1.00
31.58
B
C


ATOM
8335
SD
MET
B
475
57.511
35.151
−35.483
1.00
49.68
B
S


ATOM
8336
CE
MET
B
475
58.323
34.357
−36.907
1.00
50.34
B
C


ATOM
8337
C
MET
B
475
54.842
38.479
−33.552
1.00
22.82
B
C


ATOM
8338
O
MET
B
475
55.349
38.556
−32.430
1.00
19.84
B
O


ATOM
8339
N
ASN
B
476
54.046
39.440
−34.052
1.00
17.77
B
N


ATOM
8340
CA
ASN
B
476
53.659
40.582
−33.228
1.00
18.07
B
C


ATOM
8341
CB
ASN
B
476
52.880
41.650
−34.014
1.00
23.11
B
C


ATOM
8342
CG
ASN
B
476
53.723
42.332
−35.068
1.00
29.55
B
C


ATOM
8343
OD1
ASN
B
476
54.943
42.257
−35.033
1.00
28.97
B
O


ATOM
8344
ND2
ASN
B
476
53.068
43.011
−36.025
1.00
29.79
B
N


ATOM
8345
C
ASN
B
476
52.819
40.151
−32.047
1.00
18.90
B
C


ATOM
8346
O
ASN
B
476
52.973
40.698
−30.950
1.00
18.05
B
O


ATOM
8347
N
LEU
B
477
51.924
39.177
−32.261
1.00
17.13
B
N


ATOM
8348
CA
LEU
B
477
51.104
38.673
−31.160
1.00
18.26
B
C


ATOM
8349
CB
LEU
B
477
50.109
37.654
−31.688
1.00
20.23
B
C


ATOM
8350
CG
LEU
B
477
49.119
37.025
−30.729
1.00
28.85
B
C


ATOM
8351
CD1
LEU
B
477
48.451
38.109
−29.830
1.00
27.90
B
C


ATOM
8352
CD2
LEU
B
477
48.055
36.211
−31.571
1.00
22.42
B
C


ATOM
8353
C
LEU
B
477
51.985
38.048
−30.058
1.00
14.52
B
C


ATOM
8354
O
LEU
B
477
51.742
38.219
−28.855
1.00
15.43
B
O


ATOM
8355
N
ILE
B
478
53.007
37.305
−30.478
1.00
16.26
B
N


ATOM
8356
CA
ILE
B
478
53.996
36.766
−29.538
1.00
14.82
B
C


ATOM
8357
CB
ILE
B
478
55.024
35.865
−30.248
1.00
15.10
B
C


ATOM
8358
CG1
ILE
B
478
54.300
34.569
−30.691
1.00
15.74
B
C


ATOM
8359
CD1
ILE
B
478
55.145
33.621
−31.585
1.00
18.13
B
C


ATOM
8360
CG2
ILE
B
478
56.258
35.554
−29.342
1.00
13.94
B
C


ATOM
8361
C
ILE
B
478
54.660
37.904
−28.752
1.00
14.69
B
C


ATOM
8362
O
ILE
B
478
54.752
37.795
−27.524
1.00
15.56
B
O


ATOM
8363
N
ASP
B
479
55.094
38.980
−29.430
1.00
14.82
B
N


ATOM
8364
CA
ASP
B
479
55.705
40.149
−28.738
1.00
15.47
B
C


ATOM
8365
CB
ASP
B
479
56.139
41.213
−29.742
1.00
22.03
B
C


ATOM
8366
CG
ASP
B
479
57.356
40.787
−30.542
1.00
30.54
B
C


ATOM
8367
OD1
ASP
B
479
58.065
39.847
−30.114
1.00
31.87
B
O


ATOM
8368
OD2
ASP
B
479
57.612
41.393
−31.600
1.00
30.28
B
O


ATOM
8369
C
ASP
B
479
54.781
40.779
−27.692
1.00
16.99
B
C


ATOM
8370
O
ASP
B
479
55.185
41.056
−26.563
1.00
16.06
B
O


ATOM
8371
N
GLU
B
480
53.531
41.003
−28.073
1.00
16.84
B
N


ATOM
8372
CA
GLU
B
480
52.565
41.593
−27.173
1.00
17.96
B
C


ATOM
8373
CB
GLU
B
480
51.256
41.896
−27.927
1.00
19.41
B
C


ATOM
8374
CG
GLU
B
480
51.372
43.053
−28.936
1.00
30.52
B
C


ATOM
8375
CD
GLU
B
480
51.945
44.350
−28.305
1.00
34.11
B
C


ATOM
8376
OE1
GLU
B
480
51.388
44.805
−27.282
1.00
36.99
B
O


ATOM
8377
OE2
GLU
B
480
52.974
44.879
−28.796
1.00
41.37
B
O


ATOM
8378
C
GLU
B
480
52.305
40.686
−25.974
1.00
17.57
B
C


ATOM
8379
O
GLU
B
480
52.169
41.164
−24.855
1.00
15.62
B
O


ATOM
8380
N
THR
B
481
52.256
39.375
−26.206
1.00
14.91
B
N


ATOM
8381
CA
THR
B
481
52.072
38.432
−25.121
1.00
16.01
B
C


ATOM
8382
CB
THR
B
481
51.847
37.006
−25.655
1.00
16.05
B
C


ATOM
8383
OG1
THR
B
481
50.733
37.017
−26.574
1.00
19.54
B
O


ATOM
8384
CG2
THR
B
481
51.550
36.061
−24.491
1.00
18.71
B
C


ATOM
8385
C
THR
B
481
53.248
38.456
−24.151
1.00
16.16
B
C


ATOM
8386
O
THR
B
481
53.053
38.430
−22.954
1.00
16.06
B
O


ATOM
8387
N
TRP
B
482
54.467
38.549
−24.655
1.00
13.23
B
N


ATOM
8388
CA
TRP
B
482
55.602
38.694
−23.776
1.00
13.54
B
C


ATOM
8389
CB
TRP
B
482
56.897
38.722
−24.586
1.00
14.51
B
C


ATOM
8390
CG
TRP
B
482
57.515
37.346
−24.682
1.00
17.52
B
C


ATOM
8391
CD1
TRP
B
482
57.458
36.446
−25.752
1.00
16.21
B
C


ATOM
8392
NE1
TRP
B
482
58.141
35.294
−25.441
1.00
16.16
B
N


ATOM
8393
CE2
TRP
B
482
58.681
35.367
−24.216
1.00
17.21
B
C


ATOM
8394
CD2
TRP
B
482
58.306
36.670
−23.656
1.00
14.61
B
C


ATOM
8395
CE3
TRP
B
482
58.749
37.006
−22.375
1.00
18.50
B
C


ATOM
8396
CZ3
TRP
B
482
59.537
36.077
−21.674
1.00
18.54
B
C


ATOM
8397
CH2
TRP
B
482
59.853
34.823
−22.214
1.00
17.57
B
C


ATOM
8398
CZ2
TRP
B
482
59.437
34.443
−23.492
1.00
16.54
B
C


ATOM
8399
C
TRP
B
482
55.518
39.936
−22.906
1.00
14.61
B
C


ATOM
8400
O
TRP
B
482
55.886
39.903
−21.742
1.00
15.95
B
O


ATOM
8401
N
LYS
B
483
55.022
41.024
−23.453
1.00
14.90
B
N


ATOM
8402
CA
LYS
B
483
54.925
42.281
−22.674
1.00
16.98
B
C


ATOM
8403
CB
LYS
B
483
54.442
43.426
−23.532
1.00
17.74
B
C


ATOM
8404
CG
LYS
B
483
55.379
43.817
−24.655
1.00
20.23
B
C


ATOM
8405
CD
LYS
B
483
54.759
44.930
−25.530
1.00
21.43
B
C


ATOM
8406
CE
LYS
B
483
55.736
45.408
−26.595
1.00
21.93
B
C


ATOM
8407
NZ
LYS
B
483
55.119
46.410
−27.482
1.00
28.30
B
N


ATOM
8408
C
LYS
B
483
54.016
42.105
−21.489
1.00
17.58
B
C


ATOM
8409
O
LYS
B
483
54.306
42.584
−20.391
1.00
16.44
B
O


ATOM
8410
N
LYS
B
484
52.959
41.332
−21.697
1.00
18.31
B
N


ATOM
8411
CA
LYS
B
484
52.006
41.058
−20.654
1.00
17.54
B
C


ATOM
8412
CB
LYS
B
484
50.691
40.578
−21.275
1.00
21.90
B
C


ATOM
8413
CG
LYS
B
484
50.042
41.658
−22.116
1.00
26.76
B
C


ATOM
8414
CD
LYS
B
484
48.735
41.239
−22.728
1.00
34.55
B
C


ATOM
8415
CE
LYS
B
484
48.116
42.432
−23.459
1.00
38.13
B
C


ATOM
8416
NZ
LYS
B
484
47.234
41.967
−24.542
1.00
66.26
B
N


ATOM
8417
C
LYS
B
484
52.550
40.099
−19.605
1.00
19.69
B
C


ATOM
8418
O
LYS
B
484
52.321
40.274
−18.425
1.00
17.32
B
O


ATOM
8419
N
MET
B
485
53.344
39.130
−20.033
1.00
17.84
B
N


ATOM
8420
CA
MET
B
485
53.991
38.211
−19.107
1.00
16.90
B
C


ATOM
8421
CB
MET
B
485
54.695
37.076
−19.873
1.00
16.46
B
C


ATOM
8422
CG
MET
B
485
53.741
36.144
−20.549
1.00
19.65
B
C


ATOM
8423
SD
MET
B
485
54.501
34.543
−20.977
1.00
23.37
B
S


ATOM
8424
CE
MET
B
485
55.900
35.004
−22.009
1.00
19.59
B
C


ATOM
8425
C
MET
B
485
55.001
38.974
−18.294
1.00
16.66
B
C


ATOM
8426
O
MET
B
485
55.185
38.669
−17.118
1.00
18.87
B
O


ATOM
8427
N
ASN
B
486
55.686
39.927
−18.931
1.00
18.20
B
N


ATOM
8428
CA
ASN
B
486
56.694
40.724
−18.231
1.00
19.00
B
C


ATOM
8429
CB
ASN
B
486
57.437
41.646
−19.175
1.00
17.48
B
C


ATOM
8430
CG
ASN
B
486
58.421
40.912
−20.103
1.00
16.17
B
C


ATOM
8431
OD1
ASN
B
486
58.786
39.783
−19.864
1.00
15.97
B
O


ATOM
8432
ND2
ASN
B
486
58.821
41.580
−21.198
1.00
14.17
B
N


ATOM
8433
C
ASN
B
486
56.051
41.538
−17.090
1.00
19.96
B
C


ATOM
8434
O
ASN
B
486
56.617
41.623
−15.996
1.00
20.11
B
O


ATOM
8435
N
LYS
B
487
54.873
42.112
−17.348
1.00
19.54
B
N


ATOM
8436
CA
LYS
B
487
54.162
42.870
−16.322
1.00
22.85
B
C


ATOM
8437
CB
LYS
B
487
52.925
43.572
−16.867
1.00
26.20
B
C


ATOM
8438
CG
LYS
B
487
52.250
44.443
−15.810
1.00
33.19
B
C


ATOM
8439
CD
LYS
B
487
51.323
45.475
−16.438
1.00
44.95
B
C


ATOM
8440
CE
LYS
B
487
50.482
46.180
−15.378
1.00
51.82
B
C


ATOM
8441
NZ
LYS
B
487
49.214
46.702
−15.950
1.00
58.53
B
N


ATOM
8442
C
LYS
B
487
53.731
41.979
−15.176
1.00
23.67
B
C


ATOM
8443
O
LYS
B
487
53.802
42.377
−14.020
1.00
25.23
B
O


ATOM
8444
N
GLU
B
488
53.284
40.779
−15.496
1.00
23.22
B
N


ATOM
8445
CA
GLU
B
488
52.847
39.837
−14.480
1.00
26.06
B
C


ATOM
8446
CB
GLU
B
488
52.305
38.562
−15.152
1.00
29.47
B
C


ATOM
8447
CG
GLU
B
488
51.579
37.591
−14.230
1.00
34.00
B
C


ATOM
8448
CD
GLU
B
488
50.172
38.088
−13.807
1.00
43.28
B
C


ATOM
8449
OE1
GLU
B
488
49.541
38.871
−14.550
1.00
42.03
B
O


ATOM
8450
OE2
GLU
B
488
49.697
37.699
−12.724
1.00
49.73
B
O


ATOM
8451
C
GLU
B
488
54.001
39.482
−13.525
1.00
24.22
B
C


ATOM
8452
O
GLU
B
488
53.818
39.397
−12.309
1.00
23.72
B
O


ATOM
8453
N
LYS
B
489
55.186
39.255
−14.078
1.00
20.78
B
N


ATOM
8454
CA
LYS
B
489
56.346
38.886
−13.283
1.00
19.45
B
C


ATOM
8455
CB
LYS
B
489
57.493
38.501
−14.207
1.00
19.09
B
C


ATOM
8456
CG
LYS
B
489
58.778
38.061
−13.510
1.00
22.98
B
C


ATOM
8457
CD
LYS
B
489
58.559
36.815
−12.685
1.00
25.02
B
C


ATOM
8458
CE
LYS
B
489
59.697
36.599
−11.687
1.00
24.62
B
C


ATOM
8459
NZ
LYS
B
489
60.996
36.422
−12.344
1.00
20.33
B
N


ATOM
8460
C
LYS
B
489
56.829
40.035
−12.368
1.00
22.49
B
C


ATOM
8461
O
LYS
B
489
57.313
39.801
−11.257
1.00
24.69
B
O


ATOM
8462
N
LEU
B
490
56.739
41.259
−12.876
1.00
28.37
B
N


ATOM
8463
CA
LEU
B
490
57.153
42.446
−12.137
1.00
31.85
B
C


ATOM
8464
CB
LEU
B
490
57.239
43.646
−13.072
1.00
28.43
B
C


ATOM
8465
CG
LEU
B
490
58.610
43.878
−13.638
1.00
37.28
B
C


ATOM
8466
CD1
LEU
B
490
58.501
44.897
−14.750
1.00
36.27
B
C


ATOM
8467
CD2
LEU
B
490
59.540
44.358
−12.493
1.00
31.43
B
C


ATOM
8468
C
LEU
B
490
56.135
42.821
−11.107
1.00
38.82
B
C


ATOM
8469
O
LEU
B
490
56.482
43.239
−10.009
1.00
48.72
B
O


ATOM
8470
N
GLY
B
491
54.877
42.694
−11.516
1.00
44.34
B
N


ATOM
8471
CA
GLY
B
491
53.764
43.360
−10.897
1.00
53.36
B
C


ATOM
8472
C
GLY
B
491
53.198
42.581
−9.749
1.00
65.53
B
C


ATOM
8473
O
GLY
B
491
53.934
42.042
−8.924
1.00
88.54
B
O


ATOM
8474
N
GLY
B
492
51.880
42.525
−9.691
1.00
68.71
B
N


ATOM
8475
CA
GLY
B
492
51.217
42.067
−8.490
1.00
75.80
B
C


ATOM
8476
C
GLY
B
492
50.470
40.803
−8.777
1.00
67.99
B
C


ATOM
8477
O
GLY
B
492
49.253
40.743
−8.594
1.00
62.39
B
O


ATOM
8478
N
SER
B
493
51.179
39.784
−9.244
1.00
58.58
B
N


ATOM
8479
CA
SER
B
493
50.486
38.539
−9.522
1.00
58.70
B
C


ATOM
8480
CB
SER
B
493
51.395
37.458
−10.150
1.00
58.29
B
C


ATOM
8481
OG
SER
B
493
50.630
36.373
−10.739
1.00
37.84
B
O


ATOM
8482
C
SER
B
493
49.858
38.034
−8.219
1.00
52.46
B
C


ATOM
8483
O
SER
B
493
50.355
38.275
−7.110
1.00
44.13
B
O


ATOM
8484
N
LEU
B
494
48.728
37.372
−8.397
1.00
43.71
B
N


ATOM
8485
CA
LEU
B
494
48.161
36.479
−7.423
1.00
40.14
B
C


ATOM
8486
CB
LEU
B
494
46.941
35.833
−8.075
1.00
46.56
B
C


ATOM
8487
CG
LEU
B
494
46.008
34.991
−7.227
1.00
49.26
B
C


ATOM
8488
CD1
LEU
B
494
45.033
35.910
−6.522
1.00
65.09
B
C


ATOM
8489
CD2
LEU
B
494
45.281
34.031
−8.111
1.00
48.58
B
C


ATOM
8490
C
LEU
B
494
49.196
35.414
−7.023
1.00
39.04
B
C


ATOM
8491
O
LEU
B
494
49.296
35.040
−5.855
1.00
41.27
B
O


ATOM
8492
N
PHE
B
495
50.001
34.956
−7.985
1.00
28.85
B
N


ATOM
8493
CA
PHE
B
495
50.954
33.855
−7.746
1.00
26.55
B
C


ATOM
8494
CB
PHE
B
495
51.106
33.021
−9.026
1.00
22.08
B
C


ATOM
8495
CG
PHE
B
495
49.857
32.286
−9.410
1.00
20.12
B
C


ATOM
8496
CD1
PHE
B
495
48.909
32.889
−10.218
1.00
22.68
B
C


ATOM
8497
CE1
PHE
B
495
47.742
32.225
−10.575
1.00
27.54
B
C


ATOM
8498
CZ
PHE
B
495
47.519
30.929
−10.131
1.00
31.42
B
C


ATOM
8499
CE2
PHE
B
495
48.466
30.309
−9.333
1.00
24.27
B
C


ATOM
8500
CD2
PHE
B
495
49.637
30.998
−8.973
1.00
23.92
B
C


ATOM
8501
C
PHE
B
495
52.333
34.332
−7.286
1.00
25.17
B
C


ATOM
8502
O
PHE
B
495
52.749
35.423
−7.626
1.00
26.71
B
O


ATOM
8503
N
ALA
B
496
53.042
33.486
−6.552
1.00
30.68
B
N


ATOM
8504
CA
ALA
B
496
54.394
33.797
−6.122
1.00
39.90
B
C


ATOM
8505
CB
ALA
B
496
54.884
32.751
−5.097
1.00
34.91
B
C


ATOM
8506
C
ALA
B
496
55.318
33.849
−7.351
1.00
44.23
B
C


ATOM
8507
O
ALA
B
496
55.126
33.089
−8.305
1.00
32.16
B
O


ATOM
8508
N
LYS
B
497
56.314
34.737
−7.307
1.00
39.12
B
N


ATOM
8509
CA
LYS
B
497
57.260
34.937
−8.406
1.00
40.13
B
C


ATOM
8510
CB
LYS
B
497
58.315
36.017
−8.053
1.00
40.35
B
C


ATOM
8511
CG
LYS
B
497
57.805
37.487
−8.172
1.00
46.61
B
C


ATOM
8512
CD
LYS
B
497
58.913
38.495
−7.741
1.00
50.16
B
C


ATOM
8513
CE
LYS
B
497
58.560
39.989
−7.960
1.00
58.07
B
C


ATOM
8514
NZ
LYS
B
497
59.154
40.584
−9.230
1.00
51.75
B
N


ATOM
8515
C
LYS
B
497
57.928
33.648
−8.951
1.00
33.09
B
C


ATOM
8516
O
LYS
B
497
58.100
33.541
−10.154
1.00
32.79
B
O


ATOM
8517
N
PRO
B
498
58.272
32.666
−8.097
1.00
34.88
B
N


ATOM
8518
CA
PRO
B
498
58.857
31.424
−8.626
1.00
34.68
B
C


ATOM
8519
CB
PRO
B
498
59.171
30.602
−7.371
1.00
31.62
B
C


ATOM
8520
CG
PRO
B
498
59.308
31.635
−6.278
1.00
33.00
B
C


ATOM
8521
CD
PRO
B
498
58.349
32.723
−6.621
1.00
37.97
B
C


ATOM
8522
C
PRO
B
498
57.935
30.611
−9.535
1.00
33.03
B
C


ATOM
8523
O
PRO
B
498
58.401
30.016
−10.490
1.00
29.69
B
O


ATOM
8524
N
PHE
B
499
56.641
30.575
−9.242
1.00
26.45
B
N


ATOM
8525
CA
PHE
B
499
55.722
29.917
−10.144
1.00
23.83
B
C


ATOM
8526
CB
PHE
B
499
54.387
29.588
−9.476
1.00
23.35
B
C


ATOM
8527
CG
PHE
B
499
53.423
28.939
−10.407
1.00
22.11
B
C


ATOM
8528
CD1
PHE
B
499
53.633
27.653
−10.857
1.00
23.76
B
C


ATOM
8529
CE1
PHE
B
499
52.736
27.068
−11.742
1.00
23.42
B
C


ATOM
8530
CZ
PHE
B
499
51.648
27.756
−12.177
1.00
20.10
B
C


ATOM
8531
CE2
PHE
B
499
51.450
29.024
−11.762
1.00
22.67
B
C


ATOM
8532
CD2
PHE
B
499
52.338
29.624
−10.882
1.00
22.37
B
C


ATOM
8533
C
PHE
B
499
55.487
30.763
−11.390
1.00
18.06
B
C


ATOM
8534
O
PHE
B
499
55.382
30.219
−12.471
1.00
21.21
B
O


ATOM
8535
N
VAL
B
500
55.413
32.083
−11.247
1.00
17.55
B
N


ATOM
8536
CA
VAL
B
500
55.247
32.942
−12.418
1.00
19.54
B
C


ATOM
8537
CB
VAL
B
500
55.106
34.462
−12.096
1.00
23.69
B
C


ATOM
8538
CG1
VAL
B
500
54.996
35.282
−13.401
1.00
18.81
B
C


ATOM
8539
CG2
VAL
B
500
53.876
34.723
−11.201
1.00
24.37
B
C


ATOM
8540
C
VAL
B
500
56.397
32.687
−13.374
1.00
19.76
B
C


ATOM
8541
O
VAL
B
500
56.198
32.551
−14.579
1.00
18.23
B
O


ATOM
8542
N
GLU
B
501
57.602
32.548
−12.832
1.00
19.34
B
N


ATOM
8543
CA
GLU
B
501
58.788
32.338
−13.640
1.00
18.85
B
C


ATOM
8544
CB
GLU
B
501
60.056
32.535
−12.787
1.00
23.38
B
C


ATOM
8545
CG
GLU
B
501
61.376
32.483
−13.558
1.00
26.47
B
C


ATOM
8546
CD
GLU
B
501
61.598
33.666
−14.504
1.00
29.42
B
C


ATOM
8547
OE1
GLU
B
501
60.802
34.625
−14.546
1.00
28.81
B
O


ATOM
8548
OE2
GLU
B
501
62.598
33.632
−15.223
1.00
30.94
B
O


ATOM
8549
C
GLU
B
501
58.796
30.942
−14.291
1.00
19.55
B
C


ATOM
8550
O
GLU
B
501
59.231
30.792
−15.437
1.00
19.89
B
O


ATOM
8551
N
THR
B
502
58.349
29.919
−13.556
1.00
18.37
B
N


ATOM
8552
CA
THR
B
502
58.256
28.569
−14.109
1.00
16.92
B
C


ATOM
8553
CB
THR
B
502
57.718
27.587
−13.057
1.00
21.51
B
C


ATOM
8554
OG1
THR
B
502
58.621
27.588
−11.958
1.00
24.18
B
O


ATOM
8555
CG2
THR
B
502
57.562
26.158
−13.617
1.00
20.21
B
C


ATOM
8556
C
THR
B
502
57.319
28.595
−15.326
1.00
16.37
B
C


ATOM
8557
O
THR
B
502
57.600
27.981
−16.357
1.00
18.24
B
O


ATOM
8558
N
ALA
B
503
56.225
29.319
−15.195
1.00
15.53
B
N


ATOM
8559
CA
ALA
B
503
55.261
29.465
−16.303
1.00
17.36
B
C


ATOM
8560
CB
ALA
B
503
53.998
30.172
−15.822
1.00
15.56
B
C


ATOM
8561
C
ALA
B
503
55.853
30.181
−17.522
1.00
14.65
B
C


ATOM
8562
O
ALA
B
503
55.721
29.727
−18.661
1.00
14.63
B
O


ATOM
8563
N
ILE
B
504
56.554
31.267
−17.285
1.00
15.97
B
N


ATOM
8564
CA
ILE
B
504
57.295
31.919
−18.352
1.00
14.89
B
C


ATOM
8565
CB
ILE
B
504
57.949
33.259
−17.883
1.00
16.17
B
C


ATOM
8566
CG1
ILE
B
504
56.853
34.250
−17.464
1.00
18.62
B
C


ATOM
8567
CD1
ILE
B
504
57.334
35.541
−16.733
1.00
20.52
B
C


ATOM
8568
CG2
ILE
B
504
58.846
33.848
−19.042
1.00
18.16
B
C


ATOM
8569
C
ILE
B
504
58.321
31.018
−18.995
1.00
15.33
B
C


ATOM
8570
O
ILE
B
504
58.513
31.077
−20.217
1.00
15.15
B
O


ATOM
8571
N
ASN
B
505
58.972
30.158
−18.206
1.00
17.39
B
N


ATOM
8572
CA
ASN
B
505
59.914
29.185
−18.775
1.00
15.96
B
C


ATOM
8573
CB
ASN
B
505
60.586
28.382
−17.665
1.00
18.93
B
C


ATOM
8574
CG
ASN
B
505
61.632
29.183
−16.921
1.00
21.82
B
C


ATOM
8575
OD1
ASN
B
505
62.105
30.224
−17.411
1.00
18.32
B
O


ATOM
8576
ND2
ASN
B
505
62.015
28.691
−15.728
1.00
15.07
B
N


ATOM
8577
C
ASN
B
505
59.292
28.226
−19.830
1.00
17.21
B
C


ATOM
8578
O
ASN
B
505
59.933
27.843
−20.826
1.00
16.83
B
O


ATOM
8579
N
LEU
B
506
58.025
27.917
−19.664
1.00
16.82
B
N


ATOM
8580
CA
LEU
B
506
57.351
27.116
−20.676
1.00
17.93
B
C


ATOM
8581
CB
LEU
B
506
55.939
26.753
−20.219
1.00
17.53
B
C


ATOM
8582
CG
LEU
B
506
55.205
25.851
−21.226
1.00
24.77
B
C


ATOM
8583
CD1
LEU
B
506
54.696
24.591
−20.494
1.00
25.28
B
C


ATOM
8584
CD2
LEU
B
506
54.067
26.637
−21.819
1.00
23.39
B
C


ATOM
8585
C
LEU
B
506
57.326
27.853
−22.028
1.00
19.12
B
C


ATOM
8586
O
LEU
B
506
57.513
27.231
−23.076
1.00
15.80
B
O


ATOM
8587
N
ALA
B
507
57.108
29.171
−22.012
1.00
18.26
B
N


ATOM
8588
CA
ALA
B
507
57.230
29.954
−23.223
1.00
16.87
B
C


ATOM
8589
CB
ALA
B
507
56.810
31.430
−22.970
1.00
18.33
B
C


ATOM
8590
C
ALA
B
507
58.645
29.923
−23.732
1.00
15.87
B
C


ATOM
8591
O
ALA
B
507
58.868
29.815
−24.924
1.00
13.91
B
O


ATOM
8592
N
ARG
B
508
59.630
30.045
−22.837
1.00
16.77
B
N


ATOM
8593
CA
ARG
B
508
61.035
29.991
−23.310
1.00
14.86
B
C


ATOM
8594
CB
ARG
B
508
62.050
30.236
−22.196
1.00
14.69
B
C


ATOM
8595
CG
ARG
B
508
61.932
31.621
−21.599
1.00
16.39
B
C


ATOM
8596
CD
ARG
B
508
62.920
31.867
−20.511
1.00
17.09
B
C


ATOM
8597
NE
ARG
B
508
62.956
33.301
−20.135
1.00
18.33
B
N


ATOM
8598
CZ
ARG
B
508
62.597
33.803
−18.962
1.00
18.87
B
C


ATOM
8599
NH1
ARG
B
508
62.152
33.027
−17.977
1.00
19.08
B
N


ATOM
8600
NH2
ARG
B
508
62.699
35.117
−18.755
1.00
22.71
B
N


ATOM
8601
C
ARG
B
508
61.330
28.671
−23.972
1.00
13.08
B
C


ATOM
8602
O
ARG
B
508
61.946
28.637
−25.019
1.00
14.84
B
O


ATOM
8603
N
GLN
B
509
60.919
27.574
−23.334
1.00
12.57
B
N


ATOM
8604
CA
GLN
B
509
61.156
26.274
−23.918
1.00
14.65
B
C


ATOM
8605
CB
GLN
B
509
60.720
25.147
−22.959
1.00
15.26
B
C


ATOM
8606
CG
GLN
B
509
61.024
23.770
−23.521
1.00
17.12
B
C


ATOM
8607
CD
GLN
B
509
62.537
23.515
−23.650
1.00
21.81
B
C


ATOM
8608
OE1
GLN
B
509
63.275
23.824
−22.759
1.00
28.15
B
O


ATOM
8609
NE2
GLN
B
509
62.966
22.966
−24.755
1.00
32.54
B
N


ATOM
8610
C
GLN
B
509
60.492
26.108
−25.299
1.00
16.44
B
C


ATOM
8611
O
GLN
B
509
61.043
25.458
−26.195
1.00
17.20
B
O


ATOM
8612
N
SER
B
510
59.331
26.731
−25.478
1.00
17.03
B
N


ATOM
8613
CA
SER
B
510
58.645
26.721
−26.761
1.00
16.45
B
C


ATOM
8614
CB
SER
B
510
57.277
27.454
−26.650
1.00
17.44
B
C


ATOM
8615
OG
SER
B
510
56.458
26.798
−25.692
1.00
20.37
B
O


ATOM
8616
C
SER
B
510
59.494
27.358
−27.830
1.00
17.70
B
C


ATOM
8617
O
SER
B
510
59.638
26.815
−28.941
1.00
15.61
B
O


ATOM
8618
N
HIS
B
511
60.059
28.522
−27.499
1.00
18.37
B
N


ATOM
8619
CA
HIS
B
511
60.914
29.239
−28.419
1.00
15.76
B
C


ATOM
8620
CB
HIS
B
511
61.379
30.593
−27.842
1.00
19.17
B
C


ATOM
8621
CG
HIS
B
511
60.337
31.704
−27.906
1.00
18.73
B
C


ATOM
8622
ND1
HIS
B
511
59.990
32.312
−29.061
1.00
18.45
B
N


ATOM
8623
CE1
HIS
B
511
59.071
33.270
−28.810
1.00
20.71
B
C


ATOM
8624
NE2
HIS
B
511
58.839
33.296
−27.491
1.00
17.90
B
N


ATOM
8625
CD2
HIS
B
511
59.622
32.357
−26.893
1.00
20.37
B
C


ATOM
8626
C
HIS
B
511
62.115
28.418
−28.800
1.00
21.48
B
C


ATOM
8627
O
HIS
B
511
62.519
28.441
−29.945
1.00
17.20
B
O


ATOM
8628
N
CYS
B
512
62.697
27.694
−27.853
1.00
16.84
B
N


ATOM
8629
CA
CYS
B
512
63.918
26.933
−28.125
1.00
19.38
B
C


ATOM
8630
CB
CYS
B
512
64.687
26.692
−26.805
1.00
19.41
B
C


ATOM
8631
SG
CYS
B
512
65.249
28.250
−26.057
1.00
25.23
B
S


ATOM
8632
C
CYS
B
512
63.617
25.580
−28.842
1.00
21.62
B
C


ATOM
8633
O
CYS
B
512
64.416
25.108
−29.623
1.00
27.01
B
O


ATOM
8634
N
THR
B
513
62.454
24.995
−28.580
1.00
22.77
B
N


ATOM
8635
CA
THR
B
513
62.062
23.732
−29.192
1.00
24.80
B
C


ATOM
8636
CB
THR
B
513
60.948
23.125
−28.403
1.00
27.16
B
C


ATOM
8637
OG1
THR
B
513
61.455
22.833
−27.122
1.00
26.52
B
O


ATOM
8638
CG2
THR
B
513
60.451
21.824
−29.037
1.00
30.46
B
C


ATOM
8639
C
THR
B
513
61.615
23.859
−30.652
1.00
27.78
B
C


ATOM
8640
O
THR
B
513
61.930
23.007
−31.472
1.00
24.42
B
O


ATOM
8641
N
TYR
B
514
60.903
24.931
−30.982
1.00
22.91
B
N


ATOM
8642
CA
TYR
B
514
60.314
25.070
−32.308
1.00
28.13
B
C


ATOM
8643
CB
TYR
B
514
58.837
25.457
−32.209
1.00
22.64
B
C


ATOM
8644
CG
TYR
B
514
58.040
24.384
−31.524
1.00
24.67
B
C


ATOM
8645
CD1
TYR
B
514
57.749
23.174
−32.166
1.00
27.20
B
C


ATOM
8646
CE1
TYR
B
514
57.040
22.177
−31.516
1.00
25.06
B
C


ATOM
8647
CZ
TYR
B
514
56.620
22.390
−30.252
1.00
25.30
B
C


ATOM
8648
OH
TYR
B
514
55.932
21.420
−29.578
1.00
25.40
B
O


ATOM
8649
CE2
TYR
B
514
56.924
23.584
−29.593
1.00
22.38
B
C


ATOM
8650
CD2
TYR
B
514
57.605
24.544
−30.230
1.00
24.03
B
C


ATOM
8651
C
TYR
B
514
61.116
26.060
−33.107
1.00
34.01
B
C


ATOM
8652
O
TYR
B
514
60.846
27.240
−33.093
1.00
41.72
B
O


ATOM
8653
N
HIS
B
515
62.112
25.527
−33.808
1.00
49.16
B
N


ATOM
8654
CA
HIS
B
515
63.076
26.288
−34.597
1.00
47.01
B
C


ATOM
8655
CB
HIS
B
515
64.427
26.142
−33.925
1.00
42.91
B
C


ATOM
8656
CG
HIS
B
515
64.872
24.700
−33.790
1.00
44.52
B
C


ATOM
8657
ND1
HIS
B
515
64.972
24.077
−32.597
1.00
42.52
B
N


ATOM
8658
CE1
HIS
B
515
65.346
22.800
−32.789
1.00
37.27
B
C


ATOM
8659
NE2
HIS
B
515
65.496
22.605
−34.112
1.00
49.63
B
N


ATOM
8660
CD2
HIS
B
515
65.194
23.748
−34.758
1.00
38.43
B
C


ATOM
8661
C
HIS
B
515
63.108
25.718
−36.014
1.00
55.54
B
C


ATOM
8662
O
HIS
B
515
62.259
24.870
−36.346
1.00
42.46
B
O


ATOM
8663
N
ASN
B
516
64.073
26.174
−36.841
1.00
69.26
B
N


ATOM
8664
CA
ASN
B
516
64.386
25.594
−38.181
1.00
70.09
B
C


ATOM
8665
CB
ASN
B
516
63.740
26.423
−39.291
1.00
77.49
B
C


ATOM
8666
CG
ASN
B
516
62.232
26.459
−39.195
1.00
88.07
B
C


ATOM
8667
OD1
ASN
B
516
61.560
25.453
−39.418
1.00
92.86
B
O


ATOM
8668
ND2
ASN
B
516
61.689
27.627
−38.876
1.00
90.10
B
N


ATOM
8669
C
ASN
B
516
65.886
25.510
−38.508
1.00
51.85
B
C


ATOM
8670
O
ASN
B
516
66.686
24.937
−37.766
1.00
53.73
B
O


ATOM
8671
N
THR
B
521
73.370
24.373
−39.725
1.00
72.27
B
N


ATOM
8672
CA
THR
B
521
72.845
23.006
−39.683
1.00
70.10
B
C


ATOM
8673
CB
THR
B
521
73.400
22.213
−38.468
1.00
69.26
B
C


ATOM
8674
OG1
THR
B
521
72.791
22.682
−37.254
1.00
53.07
B
O


ATOM
8675
CG2
THR
B
521
74.955
22.328
−38.403
1.00
50.45
B
C


ATOM
8676
C
THR
B
521
71.304
23.002
−39.715
1.00
67.88
B
C


ATOM
8677
O
THR
B
521
70.686
24.063
−39.849
1.00
74.48
B
O


ATOM
8678
N
SER
B
522
70.698
21.815
−39.611
1.00
56.47
B
N


ATOM
8679
CA
SER
B
522
69.273
21.617
−39.923
1.00
54.25
B
C


ATOM
8680
CB
SER
B
522
69.116
20.434
−40.877
1.00
54.80
B
C


ATOM
8681
OG
SER
B
522
68.903
19.225
−40.162
1.00
48.21
B
O


ATOM
8682
C
SER
B
522
68.420
21.353
−38.671
1.00
70.09
B
C


ATOM
8683
O
SER
B
522
68.974
21.157
−37.590
1.00
65.77
B
O


ATOM
8684
N
PRO
B
523
67.071
21.325
−38.823
1.00
69.30
B
N


ATOM
8685
CA
PRO
B
523
66.136
21.170
−37.693
1.00
69.67
B
C


ATOM
8686
CB
PRO
B
523
64.762
21.160
−38.374
1.00
63.00
B
C


ATOM
8687
CG
PRO
B
523
64.958
21.930
−39.609
1.00
63.11
B
C


ATOM
8688
CD
PRO
B
523
66.338
21.591
−40.077
1.00
69.18
B
C


ATOM
8689
C
PRO
B
523
66.316
19.907
−36.834
1.00
68.39
B
C


ATOM
8690
O
PRO
B
523
66.474
20.009
−35.604
1.00
45.02
B
O


ATOM
8691
N
ASP
B
524
66.286
18.735
−37.461
1.00
58.00
B
N


ATOM
8692
CA
ASP
B
524
66.439
17.479
−36.717
1.00
59.59
B
C


ATOM
8693
CB
ASP
B
524
66.231
16.258
−37.625
1.00
65.22
B
C


ATOM
8694
CG
ASP
B
524
64.765
15.999
−37.929
1.00
72.73
B
C


ATOM
8695
OD1
ASP
B
524
63.895
16.413
−37.126
1.00
73.64
B
O


ATOM
8696
OD2
ASP
B
524
64.483
15.376
−38.973
1.00
63.61
B
O


ATOM
8697
C
ASP
B
524
67.796
17.380
−36.047
1.00
47.88
B
C


ATOM
8698
O
ASP
B
524
67.895
16.867
−34.935
1.00
40.13
B
O


ATOM
8699
N
GLU
B
525
68.832
17.867
−36.728
1.00
42.68
B
N


ATOM
8700
CA
GLU
B
525
70.191
17.832
−36.191
1.00
43.58
B
C


ATOM
8701
CB
GLU
B
525
71.236
18.059
−37.299
1.00
50.60
B
C


ATOM
8702
CG
GLU
B
525
72.085
16.818
−37.598
1.00
65.78
B
C


ATOM
8703
CD
GLU
B
525
72.920
16.384
−36.395
1.00
73.95
B
C


ATOM
8704
OE1
GLU
B
525
73.972
17.004
−36.150
1.00
81.89
B
O


ATOM
8705
OE2
GLU
B
525
72.521
15.434
−35.688
1.00
69.88
B
O


ATOM
8706
C
GLU
B
525
70.397
18.822
−35.033
1.00
37.64
B
C


ATOM
8707
O
GLU
B
525
71.010
18.477
−34.026
1.00
40.61
B
O


ATOM
8708
N
LEU
B
526
69.894
20.041
−35.182
1.00
42.79
B
N


ATOM
8709
CA
LEU
B
526
69.943
21.033
−34.098
1.00
42.76
B
C


ATOM
8710
CB
LEU
B
526
69.300
22.355
−34.541
1.00
39.65
B
C


ATOM
8711
CG
LEU
B
526
69.491
23.607
−33.677
1.00
35.52
B
C


ATOM
8712
CD1
LEU
B
526
70.952
23.927
−33.408
1.00
35.56
B
C


ATOM
8713
CD2
LEU
B
526
68.793
24.781
−34.375
1.00
36.01
B
C


ATOM
8714
C
LEU
B
526
69.250
20.487
−32.838
1.00
40.04
B
C


ATOM
8715
O
LEU
B
526
69.803
20.593
−31.741
1.00
30.06
B
O


ATOM
8716
N
THR
B
527
68.066
19.880
−32.994
1.00
33.52
B
N


ATOM
8717
CA
THR
B
527
67.353
19.288
−31.841
1.00
36.91
B
C


ATOM
8718
CB
THR
B
527
65.984
18.666
−32.211
1.00
35.04
B
C


ATOM
8719
OG1
THR
B
527
65.115
19.689
−32.691
1.00
38.19
B
O


ATOM
8720
CG2
THR
B
527
65.325
18.003
−30.998
1.00
30.65
B
C


ATOM
8721
C
THR
B
527
68.188
18.226
−31.157
1.00
39.79
B
C


ATOM
8722
O
THR
B
527
68.293
18.197
−29.931
1.00
34.09
B
O


ATOM
8723
N
ARG
B
528
68.804
17.349
−31.936
1.00
37.41
B
N


ATOM
8724
CA
ARG
B
528
69.547
16.269
−31.307
1.00
36.55
B
C


ATOM
8725
CB
ARG
B
528
69.831
15.137
−32.294
1.00
52.60
B
C


ATOM
8726
CG
ARG
B
528
69.701
13.738
−31.660
1.00
69.87
B
C


ATOM
8727
CD
ARG
B
528
70.156
12.662
−32.631
1.00
87.21
B
C


ATOM
8728
NE
ARG
B
528
71.397
13.075
−33.283
1.00
95.91
B
N


ATOM
8729
CZ
ARG
B
528
72.604
13.062
−32.715
1.00
88.77
B
C


ATOM
8730
NH1
ARG
B
528
72.775
12.631
−31.465
1.00
82.15
B
N


ATOM
8731
NH2
ARG
B
528
73.655
13.481
−33.413
1.00
77.77
B
N


ATOM
8732
C
ARG
B
528
70.841
16.771
−30.634
1.00
32.41
B
C


ATOM
8733
O
ARG
B
528
71.224
16.238
−29.600
1.00
32.77
B
O


ATOM
8734
N
LYS
B
529
71.496
17.776
−31.220
1.00
28.56
B
N


ATOM
8735
CA
LYS
B
529
72.665
18.432
−30.595
1.00
38.61
B
C


ATOM
8736
CB
LYS
B
529
73.287
19.491
−31.528
1.00
41.18
B
C


ATOM
8737
CG
LYS
B
529
74.196
18.949
−32.605
1.00
50.54
B
C


ATOM
8738
CD
LYS
B
529
74.880
20.083
−33.355
1.00
54.89
B
C


ATOM
8739
CE
LYS
B
529
75.945
19.563
−34.303
1.00
56.41
B
C


ATOM
8740
NZ
LYS
B
529
76.220
20.534
−35.406
1.00
61.61
B
N


ATOM
8741
C
LYS
B
529
72.292
19.099
−29.261
1.00
31.73
B
C


ATOM
8742
O
LYS
B
529
72.981
18.930
−28.259
1.00
43.25
B
O


ATOM
8743
N
ARG
B
530
71.213
19.867
−29.262
1.00
37.56
B
N


ATOM
8744
CA
ARG
B
530
70.752
20.526
−28.043
1.00
37.61
B
C


ATOM
8745
CB
ARG
B
530
69.570
21.442
−28.328
1.00
38.98
B
C


ATOM
8746
CG
ARG
B
530
69.957
22.687
−29.108
1.00
32.36
B
C


ATOM
8747
CD
ARG
B
530
68.774
23.588
−29.298
1.00
32.92
B
C


ATOM
8748
NE
ARG
B
530
69.178
24.845
−29.909
1.00
33.71
B
N


ATOM
8749
CZ
ARG
B
530
68.344
25.793
−30.318
1.00
38.82
B
C


ATOM
8750
NH1
ARG
B
530
67.033
25.649
−30.179
1.00
45.15
B
N


ATOM
8751
NH2
ARG
B
530
68.825
26.897
−30.887
1.00
42.27
B
N


ATOM
8752
C
ARG
B
530
70.398
19.489
−26.987
1.00
41.72
B
C


ATOM
8753
O
ARG
B
530
70.853
19.595
−25.852
1.00
39.24
B
O


ATOM
8754
N
VAL
B
531
69.624
18.469
−27.360
1.00
36.28
B
N


ATOM
8755
CA
VAL
B
531
69.275
17.393
−26.406
1.00
42.76
B
C


ATOM
8756
CB
VAL
B
531
68.363
16.297
−27.037
1.00
42.52
B
C


ATOM
8757
CG1
VAL
B
531
68.414
15.007
−26.226
1.00
43.01
B
C


ATOM
8758
CG2
VAL
B
531
66.938
16.807
−27.152
1.00
36.09
B
C


ATOM
8759
C
VAL
B
531
70.512
16.756
−25.791
1.00
38.29
B
C


ATOM
8760
O
VAL
B
531
70.561
16.501
−24.590
1.00
47.98
B
O


ATOM
8761
N
LEU
B
532
71.520
16.505
−26.617
1.00
45.39
B
N


ATOM
8762
CA
LEU
B
532
72.771
15.923
−26.146
1.00
42.10
B
C


ATOM
8763
CB
LEU
B
532
73.647
15.501
−27.333
1.00
52.99
B
C


ATOM
8764
CG
LEU
B
532
73.599
14.022
−27.730
1.00
55.07
B
C


ATOM
8765
CD1
LEU
B
532
72.167
13.491
−27.918
1.00
53.47
B
C


ATOM
8766
CD2
LEU
B
532
74.409
13.851
−28.987
1.00
50.51
B
C


ATOM
8767
C
LEU
B
532
73.592
16.835
−25.214
1.00
43.28
B
C


ATOM
8768
O
LEU
B
532
74.228
16.348
−24.268
1.00
37.39
B
O


ATOM
8769
N
SER
B
533
73.608
18.140
−25.498
1.00
35.64
B
N


ATOM
8770
CA
SER
B
533
74.416
19.084
−24.725
1.00
30.10
B
C


ATOM
8771
CB
SER
B
533
74.637
20.371
−25.517
1.00
31.45
B
C


ATOM
8772
OG
SER
B
533
73.422
21.073
−25.710
1.00
35.60
B
O


ATOM
8773
C
SER
B
533
73.734
19.408
−23.410
1.00
25.74
B
C


ATOM
8774
O
SER
B
533
74.375
19.842
−22.464
1.00
27.81
B
O


ATOM
8775
N
VAL
B
534
72.416
19.227
−23.351
1.00
26.13
B
N


ATOM
8776
CA
VAL
B
534
71.686
19.472
−22.117
1.00
22.57
B
C


ATOM
8777
CB
VAL
B
534
70.295
20.062
−22.402
1.00
20.39
B
C


ATOM
8778
CG1
VAL
B
534
69.458
20.157
−21.128
1.00
20.01
B
C


ATOM
8779
CG2
VAL
B
534
70.435
21.421
−23.049
1.00
21.72
B
C


ATOM
8780
C
VAL
B
534
71.590
18.219
−21.242
1.00
27.30
B
C


ATOM
8781
O
VAL
B
534
71.820
18.299
−20.041
1.00
25.45
B
O


ATOM
8782
N
ILE
B
535
71.276
17.062
−21.835
1.00
27.49
B
N


ATOM
8783
CA
ILE
B
535
71.005
15.850
−21.048
1.00
28.80
B
C


ATOM
8784
CB
ILE
B
535
69.805
15.028
−21.613
1.00
35.69
B
C


ATOM
8785
CG1
ILE
B
535
68.533
15.879
−21.713
1.00
35.37
B
C


ATOM
8786
CD1
ILE
B
535
68.029
16.446
−20.395
1.00
35.74
B
C


ATOM
8787
CG2
ILE
B
535
69.548
13.793
−20.741
1.00
36.67
B
C


ATOM
8788
C
ILE
B
535
72.178
14.879
−20.940
1.00
27.66
B
C


ATOM
8789
O
ILE
B
535
72.493
14.392
−19.857
1.00
31.72
B
O


ATOM
8790
N
THR
B
536
72.826
14.571
−22.053
1.00
32.60
B
N


ATOM
8791
CA
THR
B
536
73.616
13.337
−22.083
1.00
32.47
B
C


ATOM
8792
CB
THR
B
536
73.151
12.420
−23.235
1.00
46.51
B
C


ATOM
8793
OG1
THR
B
536
72.860
13.200
−24.393
1.00
38.77
B
O


ATOM
8794
CG2
THR
B
536
71.850
11.698
−22.839
1.00
42.92
B
C


ATOM
8795
C
THR
B
536
75.138
13.530
−21.989
1.00
33.92
B
C


ATOM
8796
O
THR
B
536
75.786
12.819
−21.227
1.00
31.82
B
O


ATOM
8797
N
GLU
B
537
75.708
14.503
−22.691
1.00
34.30
B
N


ATOM
8798
CA
GLU
B
537
77.162
14.695
−22.683
1.00
38.05
B
C


ATOM
8799
CB
GLU
B
537
77.630
15.026
−24.093
1.00
47.74
B
C


ATOM
8800
CG
GLU
B
537
77.392
13.899
−25.087
1.00
61.00
B
C


ATOM
8801
CD
GLU
B
537
77.678
14.319
−26.508
1.00
66.39
B
C


ATOM
8802
OE1
GLU
B
537
78.858
14.554
−26.826
1.00
64.96
B
O


ATOM
8803
OE2
GLU
B
537
76.721
14.421
−27.303
1.00
86.24
B
O


ATOM
8804
C
GLU
B
537
77.596
15.814
−21.727
1.00
36.26
B
C


ATOM
8805
O
GLU
B
537
77.059
16.903
−21.796
1.00
34.09
B
O


ATOM
8806
N
PRO
B
538
78.570
15.554
−20.837
1.00
32.46
B
N


ATOM
8807
CA
PRO
B
538
79.053
16.656
−20.014
1.00
30.79
B
C


ATOM
8808
CB
PRO
B
538
79.882
15.959
−18.940
1.00
29.64
B
C


ATOM
8809
CG
PRO
B
538
80.360
14.729
−19.591
1.00
33.98
B
C


ATOM
8810
CD
PRO
B
538
79.264
14.298
−20.523
1.00
39.03
B
C


ATOM
8811
C
PRO
B
538
79.883
17.654
−20.818
1.00
28.97
B
C


ATOM
8812
O
PRO
B
538
80.301
17.358
−21.933
1.00
31.95
B
O


ATOM
8813
N
ILE
B
539
80.041
18.857
−20.277
1.00
24.60
B
N


ATOM
8814
CA
ILE
B
539
80.912
19.871
−20.859
1.00
25.40
B
C


ATOM
8815
CB
ILE
B
539
80.671
21.276
−20.198
1.00
24.58
B
C


ATOM
8816
CG1
ILE
B
539
79.328
21.825
−20.687
1.00
20.47
B
C


ATOM
8817
CD1
ILE
B
539
78.935
23.187
−20.171
1.00
18.78
B
C


ATOM
8818
CG2
ILE
B
539
81.789
22.265
−20.533
1.00
21.75
B
C


ATOM
8819
C
ILE
B
539
82.333
19.422
−20.609
1.00
23.93
B
C


ATOM
8820
O
ILE
B
539
82.615
18.892
−19.530
1.00
22.47
B
O


ATOM
8821
N
LEU
B
540
83.219
19.647
−21.578
1.00
23.76
B
N


ATOM
8822
CA
LEU
B
540
84.613
19.165
−21.483
1.00
29.28
B
C


ATOM
8823
CB
LEU
B
540
85.426
19.455
−22.770
1.00
31.78
B
C


ATOM
8824
CG
LEU
B
540
85.142
18.637
−24.047
1.00
37.56
B
C


ATOM
8825
CD1
LEU
B
540
86.028
19.094
−25.222
1.00
35.30
B
C


ATOM
8826
CD2
LEU
B
540
85.330
17.126
−23.810
1.00
35.88
B
C


ATOM
8827
C
LEU
B
540
85.284
19.824
−20.287
1.00
28.87
B
C


ATOM
8828
O
LEU
B
540
85.139
21.032
−20.076
1.00
28.27
B
O


ATOM
8829
N
PRO
B
541
86.032
19.039
−19.503
1.00
30.18
B
N


ATOM
8830
CA
PRO
B
541
86.569
19.564
−18.256
1.00
27.81
B
C


ATOM
8831
CB
PRO
B
541
87.372
18.392
−17.677
1.00
31.70
B
C


ATOM
8832
CG
PRO
B
541
87.511
17.412
−18.771
1.00
31.03
B
C


ATOM
8833
CD
PRO
B
541
86.440
17.643
−19.752
1.00
30.45
B
C


ATOM
8834
C
PRO
B
541
87.465
20.781
−18.420
1.00
23.81
B
C


ATOM
8835
O
PRO
B
541
88.036
21.023
−19.493
1.00
23.24
B
O


ATOM
8836
N
PHE
B
542
87.570
21.544
−17.340
1.00
23.63
B
N


ATOM
8837
CA
PHE
B
542
88.428
22.719
−17.303
1.00
25.63
B
C


ATOM
8838
CB
PHE
B
542
88.188
23.461
−15.992
1.00
20.98
B
C


ATOM
8839
CG
PHE
B
542
89.075
24.646
−15.805
1.00
22.67
B
C


ATOM
8840
CD1
PHE
B
542
88.875
25.798
−16.545
1.00
24.18
B
C


ATOM
8841
CE1
PHE
B
542
89.696
26.892
−16.381
1.00
26.40
B
C


ATOM
8842
CZ
PHE
B
542
90.745
26.847
−15.464
1.00
26.09
B
C


ATOM
8843
CE2
PHE
B
542
90.956
25.700
−14.730
1.00
28.04
B
C


ATOM
8844
CD2
PHE
B
542
90.118
24.607
−14.901
1.00
24.54
B
C


ATOM
8845
C
PHE
B
542
89.923
22.335
−17.404
1.00
24.57
B
C


ATOM
8846
O
PHE
B
542
90.354
21.435
−16.718
1.00
26.15
B
O


ATOM
8847
N
GLU
B
543
90.691
23.048
−18.229
1.00
30.05
B
N


ATOM
8848
CA
GLU
B
543
92.144
22.847
−18.358
1.00
45.33
B
C


ATOM
8849
CB
GLU
B
543
92.475
22.331
−19.754
1.00
51.76
B
C


ATOM
8850
CG
GLU
B
543
92.258
20.848
−19.967
1.00
58.60
B
C


ATOM
8851
CD
GLU
B
543
92.868
20.385
−21.281
1.00
70.33
B
C


ATOM
8852
OE1
GLU
B
543
92.308
20.724
−22.346
1.00
78.28
B
O


ATOM
8853
OE2
GLU
B
543
93.915
19.701
−21.249
1.00
62.47
B
O


ATOM
8854
C
GLU
B
543
92.921
24.154
−18.139
1.00
55.07
B
C


ATOM
8855
O
GLU
B
543
92.409
25.240
−18.410
1.00
68.55
B
O


ATOM
8856
N
ARG
B
544
94.161
24.048
−17.673
1.00
66.21
B
N


ATOM
8857
CA
ARG
B
544
95.063
25.206
−17.600
1.00
66.46
B
C


ATOM
8858
CB
ARG
B
544
96.449
24.783
−17.115
1.00
72.05
B
C


ATOM
8859
CG
ARG
B
544
96.630
24.852
−15.618
1.00
82.51
B
C


ATOM
8860
CD
ARG
B
544
96.466
26.273
−15.082
1.00
82.85
B
C


ATOM
8861
NE
ARG
B
544
95.163
26.517
−14.456
1.00
83.68
B
N


ATOM
8862
CZ
ARG
B
544
94.775
26.005
−13.287
1.00
88.75
B
C


ATOM
8863
NH1
ARG
B
544
95.564
25.183
−12.604
1.00
105.01
B
N


ATOM
8864
NH2
ARG
B
544
93.583
26.307
−12.791
1.00
82.97
B
N


ATOM
8865
C
ARG
B
544
95.208
25.906
−18.947
1.00
60.40
B
C


ATOM
8866
O
ARG
B
544
95.633
27.055
−19.008
1.00
50.23
B
O


ATOM
8867
O2
EDO
C
1
50.210
−34.939
6.459
1.00
38.13

O


ATOM
8868
C2
EDO
C
1
49.011
−35.682
6.773
1.00
41.21

C


ATOM
8869
C1
EDO
C
1
49.054
−36.235
8.192
1.00
45.53

C


ATOM
8870
O1
EDO
C
1
49.986
−37.333
8.219
1.00
44.71

O


ATOM
8871
O2
EDO
C
2
63.438
−31.727
17.582
1.00
45.81

O


ATOM
8872
C2
EDO
C
2
62.577
−30.665
18.072
1.00
74.69

C


ATOM
8873
C1
EDO
C
2
61.818
−31.103
19.324
1.00
83.94

C


ATOM
8874
O1
EDO
C
2
60.663
−31.864
18.957
1.00
82.22

O


ATOM
8875
O2
EDO
C
3
35.774
−12.724
19.459
1.00
49.91

O


ATOM
8876
C2
EDO
C
3
34.695
−13.626
19.183
1.00
51.62

C


ATOM
8877
C1
EDO
C
3
35.066
−15.056
19.586
1.00
50.18

C


ATOM
8878
O1
EDO
C
3
34.908
−15.275
20.995
1.00
52.38

O


ATOM
8879
O2
EDO
C
4
55.664
−31.020
−7.478
1.00
62.16

O


ATOM
8880
C2
EDO
C
4
56.447
−32.199
−7.745
1.00
55.16

C


ATOM
8881
C1
EDO
C
4
57.841
−32.025
−7.181
1.00
46.79

C


ATOM
8882
O1
EDO
C
4
57.749
−31.794
−5.762
1.00
44.89

O


ATOM
8883
O2
EDO
C
5
59.390
40.828
−24.889
1.00
46.90

O


ATOM
8884
C2
EDO
C
5
58.426
41.563
−25.689
1.00
52.84

C


ATOM
8885
C1
EDO
C
5
58.953
42.947
−26.019
1.00
60.33

C


ATOM
8886
O1
EDO
C
5
59.696
42.902
−27.239
1.00
52.81

O


ATOM
8887
O2
EDO
C
6
67.159
24.576
−21.207
1.00
44.76

O


ATOM
8888
C2
EDO
C
6
67.371
23.331
−21.847
1.00
79.38

C


ATOM
8889
C1
EDO
C
6
66.232
23.107
−22.819
1.00
81.18

C


ATOM
8890
O1
EDO
C
6
65.631
21.876
−22.429
1.00
57.51

O


ATOM
8891
O2
EDO
C
7
42.814
23.197
−18.005
1.00
43.20

O


ATOM
8892
C2
EDO
C
7
43.711
23.598
−16.933
1.00
55.67

C


ATOM
8893
C1
EDO
C
7
42.980
23.928
−15.622
1.00
49.52

C


ATOM
8894
O1
EDO
C
7
41.983
22.938
−15.314
1.00
49.06

O


ATOM
8895
O2
EDO
C
8
65.887
35.660
−13.297
1.00
64.73

O


ATOM
8896
C2
EDO
C
8
66.494
35.632
−11.997
1.00
60.39

C


ATOM
8897
C1
EDO
C
8
65.653
36.461
−11.041
1.00
51.23

C


ATOM
8898
O1
EDO
C
8
64.285
36.084
−11.242
1.00
63.87

O


ATOM
8899
O2
EDO
C
9
75.025
−15.092
−7.645
1.00
73.04

O


ATOM
8900
C2
EDO
C
9
75.916
−15.591
−6.618
1.00
54.13

C


ATOM
8901
C1
EDO
C
9
76.282
−14.481
−5.629
1.00
62.03

C


ATOM
8902
O1
EDO
C
9
76.476
−15.036
−4.307
1.00
71.74

O


ATOM
8903
O2
EDO
C
10
37.539
−13.647
−3.807
1.00
56.73

O


ATOM
8904
C2
EDO
C
10
38.572
−14.413
−3.187
1.00
53.33

C


ATOM
8905
C1
EDO
C
10
38.080
−14.845
−1.828
1.00
66.57

C


ATOM
8906
O1
EDO
C
10
38.329
−13.862
−0.808
1.00
43.72

O


ATOM
8907
O2
EDO
C
11
62.286
22.846
−3.976
1.00
95.27

O


ATOM
8908
C2
EDO
C
11
61.432
21.701
−3.837
1.00
78.37

C


ATOM
8909
C1
EDO
C
11
60.151
21.921
−4.633
1.00
74.32

C


ATOM
8910
O1
EDO
C
11
59.125
22.519
−3.825
1.00
66.41

O


ATOM
8911
O
HOH
W
1
34.048
30.209
−35.713
1.00
30.57

O


ATOM
8912
O
HOH
W
2
72.221
30.561
−18.308
1.00
34.52

O


ATOM
8913
O
HOH
W
3
42.922
−37.541
1.254
1.00
24.96

O


ATOM
8914
O
HOH
W
4
50.386
5.471
−4.647
1.00
32.49

O


ATOM
8915
O
HOH
W
5
33.078
3.362
−9.383
1.00
54.39

O


ATOM
8916
O
HOH
W
6
52.914
44.981
−20.022
1.00
38.76

O


ATOM
8917
O
HOH
W
7
46.727
−46.708
−0.071
1.00
28.55

O


ATOM
8918
O
HOH
W
8
43.693
28.326
0.980
1.00
60.87

O


ATOM
8919
O
HOH
W
9
40.812
33.780
−15.967
1.00
55.29

O


ATOM
8920
O
HOH
W
10
34.233
−15.102
−12.393
1.00
69.73

O


ATOM
8921
O
HOH
W
11
60.558
−41.349
3.050
1.00
30.19

O


ATOM
8922
O
HOH
W
12
51.686
−35.679
−4.930
1.00
30.59

O


ATOM
8923
O
HOH
W
13
41.009
10.787
−34.962
1.00
32.13

O


ATOM
8924
O
HOH
W
14
72.510
−28.244
9.403
1.00
47.03

O


ATOM
8925
O
HOH
W
15
58.976
52.860
−18.688
1.00
44.24

O


ATOM
8926
O
HOH
W
16
38.872
−12.021
23.356
1.00
57.68

O


ATOM
8927
O
HOH
W
17
52.746
17.543
−10.057
1.00
29.72

O


ATOM
8928
O
HOH
W
18
51.072
−32.904
−1.376
1.00
29.37

O


ATOM
8929
O
HOH
W
19
63.526
−35.430
−0.774
1.00
28.10

O


ATOM
8930
O
HOH
W
20
68.058
39.373
−17.925
1.00
39.37

O


ATOM
8931
O
HOH
W
21
33.554
−10.585
−11.360
1.00
67.19

O


ATOM
8932
O
HOH
W
22
37.063
12.291
−2.365
1.00
51.31

O


ATOM
8933
O
HOH
W
23
37.950
−12.663
−6.107
1.00
35.96

O


ATOM
8934
O
HOH
W
24
59.883
20.188
−7.683
1.00
40.62

O


ATOM
8935
O
HOH
W
25
64.280
31.472
−14.859
1.00
54.99

O


ATOM
8936
O
HOH
W
26
50.836
−23.573
23.569
1.00
61.82

O


ATOM
8937
O
HOH
W
27
44.198
36.276
−15.221
1.00
47.21

O


ATOM
8938
O
HOH
W
28
27.068
−13.991
20.678
1.00
58.53

O


ATOM
8939
O
HOH
W
29
59.191
6.102
3.877
1.00
52.78

O


ATOM
8940
O
HOH
W
30
56.173
43.466
−32.287
1.00
55.42

O


ATOM
8941
O
HOH
W
31
48.255
36.614
−25.882
1.00
35.33

O


ATOM
8942
O
HOH
W
32
52.907
8.991
−11.103
1.00
35.35

O


ATOM
8943
O
HOH
W
33
78.772
40.802
−19.806
1.00
64.63

O


ATOM
8944
O
HOH
W
34
35.785
6.639
−16.100
1.00
38.23

O


ATOM
8945
O
HOH
W
35
34.929
18.983
−26.361
1.00
36.02

O


ATOM
8946
O
HOH
W
36
65.094
−28.160
−0.117
1.00
30.19

O


ATOM
8947
O
HOH
W
37
39.427
−1.601
16.456
1.00
37.99

O


ATOM
8948
O
HOH
W
38
55.902
−54.816
1.904
1.00
37.57

O


ATOM
8949
O
HOH
W
39
61.453
35.126
−30.488
1.00
46.77

O


ATOM
8950
O
HOH
W
40
44.942
4.117
−9.297
1.00
26.04

O


ATOM
8951
O
HOH
W
41
41.406
−41.347
0.269
1.00
28.33

O


ATOM
8952
O
HOH
W
42
57.974
−52.022
−9.497
1.00
40.95

O


ATOM
8953
O
HOH
W
43
42.050
21.939
−12.530
1.00
33.98

O


ATOM
8954
O
HOH
W
44
41.003
−33.274
−1.457
1.00
33.03

O


ATOM
8955
O
HOH
W
45
40.926
−47.571
5.365
1.00
32.94

O


ATOM
8956
O
HOH
W
46
49.424
−10.782
−8.514
1.00
29.03

O


ATOM
8957
O
HOH
W
47
45.277
−21.798
−15.931
1.00
55.04

O


ATOM
8958
O
HOH
W
48
51.511
−44.053
−11.372
1.00
34.36

O


ATOM
8959
O
HOH
W
49
44.630
14.779
−38.339
1.00
50.75

O


ATOM
8960
O
HOH
W
50
67.819
−51.366
2.851
1.00
60.07

O


ATOM
8961
O
HOH
W
51
38.599
22.223
−22.766
1.00
36.92

O


ATOM
8962
O
HOH
W
52
74.566
−28.008
−17.386
1.00
37.79

O


ATOM
8963
O
HOH
W
53
64.915
27.603
−18.440
1.00
30.85

O


ATOM
8964
O
HOH
W
54
47.207
−13.782
9.500
1.00
40.97

O


ATOM
8965
O
HOH
W
55
66.176
−37.909
3.811
1.00
46.95

O


ATOM
8966
O
HOH
W
56
53.336
16.841
−7.459
1.00
37.81

O


ATOM
8967
O
HOH
W
57
73.333
30.235
−30.752
1.00
43.63

O


ATOM
8968
O
HOH
W
58
33.230
18.696
−28.428
1.00
45.03

O


ATOM
8969
O
HOH
W
59
66.175
−39.931
−15.870
1.00
39.41

O


ATOM
8970
O
HOH
W
60
35.995
14.593
−42.897
1.00
65.27

O


ATOM
8971
O
HOH
W
61
58.757
13.049
−4.982
1.00
66.66

O


ATOM
8972
O
HOH
W
62
37.415
9.573
12.896
1.00
51.12

O


ATOM
8973
O
HOH
W
63
47.051
24.023
−34.571
1.00
47.66

O


ATOM
8974
O
HOH
W
64
72.806
−37.016
2.957
1.00
47.83

O


ATOM
8975
O
HOH
W
65
73.786
10.617
−12.312
1.00
52.20

O


ATOM
8976
O
HOH
W
66
40.427
12.828
10.123
1.00
52.56

O


ATOM
8977
O
HOH
W
67
35.371
−1.990
−16.124
1.00
43.14

O


ATOM
8978
O
HOH
W
68
38.863
−4.985
16.991
1.00
38.62

O


ATOM
8979
O
HOH
W
69
35.770
−4.210
−3.569
1.00
35.17

O


ATOM
8980
O
HOH
W
70
68.324
−43.580
−13.697
1.00
43.83

O


ATOM
8981
O
HOH
W
71
33.955
−13.890
2.042
1.00
40.37

O


ATOM
8982
O
HOH
W
72
61.227
−29.698
2.658
1.00
35.13

O


ATOM
8983
O
HOH
W
73
65.711
−50.865
5.677
1.00
43.64

O


ATOM
8984
O
HOH
W
74
74.357
−42.812
−10.884
1.00
44.35

O


ATOM
8985
O
HOH
W
75
45.510
−11.279
9.183
1.00
53.55

O


ATOM
8986
O
HOH
W
76
58.068
37.458
−31.933
1.00
45.05

O


ATOM
8987
O
HOH
W
77
29.944
−7.112
9.777
1.00
50.98

O


ATOM
8988
O
HOH
W
78
70.628
32.182
−4.862
1.00
50.28

O


ATOM
8989
O
HOH
W
79
49.706
−31.542
13.259
1.00
37.73

O


ATOM
8990
O
HOH
W
80
39.571
27.621
−39.892
1.00
36.81

O


ATOM
8991
O
HOH
W
81
49.230
40.718
−34.169
1.00
39.33

O


ATOM
8992
O
HOH
W
82
63.542
44.164
−6.413
1.00
48.75

O


ATOM
8993
O
HOH
W
83
75.927
−38.606
−1.097
1.00
47.25

O


ATOM
8994
O
HOH
W
84
76.193
41.852
−2.775
1.00
42.75

O


ATOM
8995
O
HOH
W
85
70.942
44.418
−21.606
1.00
42.30

O


ATOM
8996
O
HOH
W
86
73.469
−41.972
−3.523
1.00
38.48

O


ATOM
8997
O
HOH
W
87
69.367
−31.467
−16.366
1.00
53.89

O


ATOM
8998
O
HOH
W
88
54.386
16.085
−3.461
1.00
52.54

O


ATOM
8999
O
HOH
W
89
66.132
31.872
−19.085
1.00
35.71

O


ATOM
9000
O
HOH
W
90
66.404
−41.771
6.383
1.00
38.20

O


ATOM
9001
O
HOH
W
91
50.092
22.669
0.190
1.00
51.87

O


ATOM
9002
O
HOH
W
92
39.373
−39.231
0.174
1.00
35.92

O


ATOM
9003
O
HOH
W
93
65.979
−23.272
11.936
1.00
69.59

O


ATOM
9004
O
HOH
W
94
48.792
−13.384
21.980
1.00
74.36

O


ATOM
9005
O
HOH
W
95
71.412
−24.600
−16.671
1.00
36.24

O


ATOM
9006
O
HOH
W
96
56.586
26.740
−41.218
1.00
41.19

O


ATOM
9007
O
HOH
W
97
39.489
−7.587
16.107
1.00
51.95

O


ATOM
9008
O
HOH
W
98
53.647
−55.615
−0.148
1.00
51.86

O


ATOM
9009
O
HOH
W
99
46.171
−56.680
−2.479
1.00
35.43

O


ATOM
9010
O
HOH
W
100
39.972
−32.522
15.077
1.00
48.51

O


ATOM
9011
O
HOH
W
101
48.922
−24.272
21.614
1.00
54.16

O


ATOM
9012
O
HOH
W
102
74.982
40.128
−38.250
1.00
66.19

O


ATOM
9013
O
HOH
W
103
61.120
47.767
−13.833
1.00
40.35

O


ATOM
9014
O
HOH
W
104
41.768
−16.179
15.677
1.00
50.58

O


ATOM
9015
O
HOH
W
105
60.972
−37.487
−17.532
1.00
53.12

O


ATOM
9016
O
HOH
W
106
41.668
−1.875
−29.376
1.00
40.10

O


ATOM
9017
O
HOH
W
107
62.308
38.576
−13.930
1.00
39.71

O


ATOM
9018
O
HOH
W
108
52.023
−49.412
−4.168
1.00
38.73

O


ATOM
9019
O
HOH
W
109
44.425
−14.470
−21.023
1.00
59.71

O


ATOM
9020
O
HOH
W
110
68.249
−40.733
1.635
1.00
51.87

O


ATOM
9021
O
HOH
W
111
58.014
40.360
−34.091
1.00
58.79

O


ATOM
9022
O
HOH
W
112
65.473
−25.959
8.283
1.00
37.98

O


ATOM
9023
O
HOH
W
113
58.376
28.638
−34.072
1.00
37.08

O


ATOM
9024
O
HOH
W
114
40.956
20.759
−36.052
1.00
34.09

O


ATOM
9025
O
HOH
W
115
53.523
−30.166
11.765
1.00
47.29

O


ATOM
9026
O
HOH
W
116
81.018
−22.161
−3.491
1.00
48.31

O


ATOM
9027
O
HOH
W
117
77.989
31.942
−1.939
1.00
52.87

O


ATOM
9028
O
HOH
W
118
30.031
−4.641
5.412
1.00
52.50

O


ATOM
9029
O
HOH
W
119
49.668
41.842
−31.710
1.00
52.92

O


ATOM
9030
O
HOH
W
120
46.700
19.419
−30.652
1.00
53.45

O


ATOM
9031
O
HOH
W
121
63.076
−29.920
0.218
1.00
34.61

O


ATOM
9032
O
HOH
W
122
63.859
26.049
−4.564
1.00
64.19

O


ATOM
9033
O
HOH
W
123
69.023
−54.334
7.222
1.00
83.51

O


ATOM
9034
O
HOH
W
124
65.415
−27.642
−23.447
1.00
60.02

O


ATOM
9035
O
HOH
W
125
55.626
50.586
−29.608
1.00
55.69

O


ATOM
9036
O
HOH
W
126
66.593
44.060
−2.954
1.00
68.62

O


ATOM
9037
O
HOH
W
127
58.391
−28.842
16.508
1.00
57.64

O


ATOM
9038
O
HOH
W
128
53.900
−39.356
−12.313
1.00
43.15

O


ATOM
9039
O
HOH
W
129
74.782
37.164
−27.479
1.00
44.49

O


ATOM
9040
O
HOH
W
130
90.863
34.951
−9.722
1.00
62.59

O


ATOM
9041
O
HOH
W
131
52.472
−48.905
−6.906
1.00
56.63

O


ATOM
9042
O
HOH
W
132
43.350
10.560
11.057
1.00
38.08

O


ATOM
9043
O
HOH
W
133
52.074
−50.711
14.213
1.00
54.06

O


ATOM
9044
O
HOH
W
134
70.265
−31.997
17.051
1.00
57.08

O


ATOM
9045
O
HOH
W
135
82.367
−22.862
−5.771
1.00
53.41

O


ATOM
9046
O
HOH
W
136
73.877
−43.074
−1.202
1.00
52.04

O


ATOM
9047
O
HOH
W
137
61.815
36.018
−7.588
1.00
66.58

O


ATOM
9048
O
HOH
W
138
36.147
−3.135
−8.569
1.00
54.25

O


ATOM
9049
O
HOH
W
139
38.569
−3.334
−28.598
1.00
46.22

O


ATOM
9050
O
HOH
W
140
30.675
4.623
10.151
1.00
44.33

O


ATOM
9051
O
HOH
W
141
34.344
−12.039
4.162
1.00
35.56

O


ATOM
9052
O
HOH
W
142
66.321
−28.096
12.287
1.00
44.18

O


ATOM
9053
O
HOH
W
143
39.877
17.457
−2.554
1.00
57.30

O


ATOM
9054
O
HOH
W
144
37.888
20.717
−9.842
1.00
50.69

O


ATOM
9055
O
HOH
W
145
36.597
−25.323
28.278
1.00
54.67

O


ATOM
9056
O
HOH
W
146
57.161
18.828
−5.002
1.00
58.07

O


ATOM
9057
O
HOH
W
147
62.481
−31.446
7.726
1.00
41.64

O


ATOM
9058
O
HOH
W
148
76.571
43.616
−24.789
1.00
44.73

O


ATOM
9059
O
HOH
W
149
30.688
−1.114
6.069
1.00
56.57

O


ATOM
9060
O
HOH
W
150
81.407
28.142
−1.050
1.00
38.72

O


ATOM
9061
O
HOH
W
151
77.076
36.019
0.324
1.00
42.72

O


ATOM
9062
O
HOH
W
152
51.332
29.535
−2.522
1.00
49.96

O


ATOM
9063
O
HOH
W
153
39.612
−34.777
16.884
1.00
52.30

O


ATOM
9064
O
HOH
W
154
79.194
43.959
0.289
1.00
43.48

O


ATOM
9065
O
HOH
W
155
38.215
13.169
−10.040
1.00
64.98

O


ATOM
9066
O
HOH
W
156
56.920
35.850
−4.782
1.00
60.82

O


ATOM
9067
O
HOH
W
157
47.053
−24.557
23.296
1.00
49.95

O


ATOM
9068
O
HOH
W
158
58.327
5.069
−30.924
1.00
79.38

O


ATOM
9069
O
HOH
W
159
64.930
46.968
−37.558
1.00
56.21

O


ATOM
9070
O
HOH
W
160
57.306
−26.687
−3.825
1.00
45.16

O


ATOM
9071
O
HOH
W
161
36.627
−11.455
17.346
1.00
37.75

O


ATOM
9072
O
HOH
W
162
50.199
44.936
−19.825
1.00
51.53

O


ATOM
9073
O
HOH
W
163
54.531
9.807
−8.888
1.00
57.92

O


ATOM
9074
O
HOH
W
164
78.331
52.654
−23.946
1.00
46.37

O


ATOM
9075
O
HOH
W
165
73.905
−38.892
−3.033
1.00
49.22

O


ATOM
9076
O
HOH
W
166
50.113
−10.237
−11.005
1.00
29.80

O


ATOM
9077
O
HOH
W
167
36.232
0.508
−31.397
1.00
45.29

O


ATOM
9078
O
HOH
W
168
43.592
39.061
−19.142
1.00
63.60

O


ATOM
9079
O
HOH
W
169
40.214
−33.998
12.608
1.00
57.97

O


ATOM
9080
O
HOH
W
170
75.916
−38.941
−4.757
1.00
58.71

O


ATOM
9081
O
HOH
W
171
50.926
30.569
−48.782
1.00
68.05

O


ATOM
9082
O
HOH
W
172
30.965
12.611
−18.907
1.00
48.18

O


ATOM
9083
O
HOH
W
173
65.768
32.879
−34.997
1.00
59.97

O


ATOM
9084
O
HOH
W
174
67.132
−46.375
−14.735
1.00
63.90

O


ATOM
9085
O
HOH
W
175
30.868
−31.797
22.531
1.00
71.45

O


ATOM
9086
O
HOH
W
176
37.574
17.226
−11.259
1.00
44.53

O


ATOM
9087
O
HOH
W
177
67.951
28.566
−6.687
1.00
62.21

O


ATOM
9088
O
HOH
W
178
51.731
−23.089
15.681
1.00
44.07

O


ATOM
9089
O
HOH
W
179
61.714
18.275
−6.221
1.00
41.96

O


ATOM
9090
O
HOH
W
180
38.431
13.065
−7.226
1.00
51.80

O


ATOM
9091
O
HOH
W
181
54.783
3.722
12.486
1.00
49.70

O


ATOM
9092
O
HOH
W
182
81.778
31.669
−29.328
1.00
70.71

O


ATOM
9093
O
HOH
W
183
72.037
35.490
7.499
1.00
52.36

O


ATOM
9094
O
HOH
W
184
30.818
6.946
−28.568
1.00
42.29

O


ATOM
9095
O
HOH
W
185
89.548
24.910
−20.320
1.00
60.31

O


ATOM
9096
O
HOH
W
186
31.480
−7.942
7.728
1.00
41.20

O


ATOM
9097
O
HOH
W
187
35.972
10.716
8.864
1.00
61.98

O


ATOM
9098
O
HOH
W
188
73.905
−14.913
−2.316
1.00
46.00

O


ATOM
9099
O
HOH
W
189
78.890
44.704
−4.758
1.00
47.35

O


ATOM
9100
O
HOH
W
190
47.865
33.986
−3.697
1.00
71.46

O


ATOM
9101
O
HOH
W
191
55.489
−51.636
−8.793
1.00
51.44

O


ATOM
9102
O
HOH
W
192
44.629
34.253
−47.662
1.00
52.49

O


ATOM
9103
O
HOH
W
193
36.321
−3.150
−11.179
1.00
53.77

O


ATOM
9104
O
HOH
W
194
75.543
27.909
−1.152
1.00
62.47

O


ATOM
9105
O
HOH
W
195
68.920
32.752
−21.140
1.00
42.66

O


ATOM
9106
O
HOH
W
196
56.053
16.201
4.167
1.00
64.28

O


ATOM
9107
O
HOH
W
197
70.643
32.752
−18.624
1.00
40.54

O


ATOM
9108
O
HOH
W
198
42.990
18.891
−36.337
1.00
43.70

O


ATOM
9109
O
HOH
W
199
32.339
21.015
−35.934
1.00
46.78

O


ATOM
9110
O
HOH
W
200
64.041
−53.498
6.127
1.00
45.10

O


ATOM
9111
O
HOH
W
201
37.492
−2.289
−14.229
1.00
43.61

O


ATOM
9112
O
HOH
W
202
54.643
2.234
−30.940
1.00
54.31

O


ATOM
9113
O
HOH
W
203
81.792
34.267
−28.947
1.00
63.73

O


ATOM
9114
O
HOH
W
204
51.574
−47.862
15.312
1.00
52.96

O


ATOM
9115
O
HOH
W
205
66.116
−0.774
−1.663
1.00
57.05

O


ATOM
9116
O
HOH
W
206
63.489
−29.240
−13.484
1.00
47.17

O


ATOM
9117
O
HOH
W
207
33.774
−11.564
21.850
1.00
53.05

O


ATOM
9118
O
HOH
W
208
33.410
5.065
2.929
1.00
51.28

O


ATOM
9119
O
HOH
W
209
89.488
19.709
−15.078
1.00
67.84

O


ATOM
9120
O
HOH
W
210
51.136
−34.897
−7.810
1.00
46.73

O


ATOM
9121
O
HOH
W
211
39.578
31.108
−40.140
1.00
39.00

O


ATOM
9122
O
HOH
W
212
55.752
−41.092
20.935
1.00
51.13

O


ATOM
9123
O
HOH
W
213
63.192
38.662
−8.121
1.00
46.18

O


ATOM
9124
O
HOH
W
214
75.303
−41.780
−5.954
1.00
63.51

O


ATOM
9125
O
HOH
W
215
67.066
−29.506
14.424
1.00
48.90

O


ATOM
9126
O
HOH
W
216
84.007
−41.435
3.877
1.00
84.50

O


ATOM
9127
O
HOH
W
217
33.659
−6.085
−4.081
1.00
58.14

O


ATOM
9128
O
HOH
W
218
54.424
51.143
−27.170
1.00
66.78

O


ATOM
9129
O
HOH
W
219
31.233
14.822
−42.523
1.00
66.06

O


ATOM
9130
O
HOH
W
220
59.996
46.699
−32.009
1.00
48.66

O


ATOM
9131
O
HOH
W
221
30.554
13.140
−16.210
1.00
61.86

O


ATOM
9132
O
HOH
W
222
44.203
−28.493
−14.505
1.00
77.63

O


ATOM
9133
O
HOH
W
223
30.289
8.081
−13.588
1.00
54.11

O


ATOM
9134
O
HOH
W
224
50.274
−42.317
−4.855
1.00
26.53

O


ATOM
9135
O
HOH
W
225
28.651
−3.712
7.966
1.00
54.69

O


ATOM
9136
O
HOH
W
226
67.116
39.647
−23.789
1.00
37.76

O


ATOM
9137
O
HOH
W
227
37.656
−21.694
−17.940
1.00
61.68

O


ATOM
9138
O
HOH
W
228
91.647
34.394
−14.107
1.00
45.15

O


ATOM
9139
O
HOH
W
229
41.028
35.508
−7.118
1.00
61.75

O


ATOM
9140
O
HOH
W
230
40.248
−28.559
6.969
1.00
38.66

O


ATOM
9141
O
HOH
W
231
54.162
−42.641
18.951
1.00
41.38

O


ATOM
9142
O
HOH
W
232
81.228
−32.085
−13.074
1.00
41.35

O


ATOM
9143
O
HOH
W
233
50.268
−35.358
−2.701
1.00
28.38

O


ATOM
9144
O
HOH
W
234
64.857
30.043
−17.341
1.00
34.77

O


ATOM
9145
O
HOH
W
235
62.674
9.209
−26.807
1.00
58.76

O


ATOM
9146
O
HOH
W
236
82.184
52.975
−10.392
1.00
61.52

O


ATOM
9147
O
HOH
W
237
36.482
−4.133
18.202
1.00
41.73

O


ATOM
9148
O
HOH
W
238
74.033
16.183
−7.064
1.00
71.16

O


ATOM
9149
O
HOH
W
239
65.741
−38.019
19.761
1.00
57.50

O


ATOM
9150
O
HOH
W
240
49.937
−21.240
22.807
1.00
46.67

O


ATOM
9151
O
HOH
W
241
34.731
−6.689
−7.318
1.00
48.62

O


ATOM
9152
O
HOH
W
242
65.731
38.382
−18.155
1.00
44.68

O


ATOM
9153
O
HOH
W
243
29.862
−2.337
4.065
1.00
59.55

O


ATOM
9154
O
HOH
W
244
45.836
−15.023
−19.205
1.00
49.02

O


ATOM
9155
O
HOH
W
245
36.589
−1.619
17.193
1.00
40.23

O


ATOM
9156
O
HOH
W
246
34.323
−10.323
−8.877
1.00
58.54

O


ATOM
9157
O
HOH
W
247
62.139
39.167
−10.432
1.00
45.40

O


ATOM
9158
O
HOH
W
248
49.939
41.406
−17.501
1.00
46.80

O


ATOM
9159
O
HOH
W
249
53.513
48.260
−26.270
1.00
60.43

O


ATOM
9160
O
HOH
W
250
73.526
23.237
−27.444
1.00
61.10

O


ATOM
9161
O
HOH
W
251
69.211
−30.970
14.209
1.00
53.73

O


ATOM
9162
O
HOH
W
252
49.336
18.864
−28.048
1.00
57.51

O


ATOM
9163
O
HOH
W
253
49.935
1.484
−13.946
1.00
37.90

O


ATOM
9164
O
HOH
W
254
30.096
3.893
5.083
1.00
66.59

O


ATOM
9165
O
HOH
W
255
39.758
−7.496
13.501
1.00
56.93

O


ATOM
9166
O
HOH
W
256
82.305
−35.291
−6.599
1.00
51.37

O


ATOM
9167
O
HOH
W
257
49.782
−39.950
8.443
1.00
31.76

O


ATOM
9168
O
HOH
W
258
27.976
−9.654
14.307
1.00
48.06

O


ATOM
9169
O
HOH
W
259
47.094
−21.051
28.763
1.00
63.75

O


ATOM
9170
O
HOH
W
260
41.251
14.226
−35.483
1.00
35.07

O


ATOM
9171
O
HOH
W
261
42.481
24.274
−1.324
1.00
69.59

O


ATOM
9172
O
HOH
W
262
51.446
20.748
−28.017
1.00
45.64

O


ATOM
9173
O
HOH
W
263
72.148
25.241
−29.773
1.00
65.72

O


ATOM
9174
O
HOH
W
264
39.401
11.992
−4.214
1.00
49.54

O


ATOM
9175
O
HOH
W
265
32.275
12.617
−24.662
1.00
50.59

O


ATOM
9176
O
HOH
W
266
55.250
9.269
−25.684
1.00
52.64

O


ATOM
9177
O
HOH
W
267
58.661
44.938
−30.151
1.00
61.24

O


ATOM
9178
O
HOH
W
268
83.116
−36.414
−4.312
1.00
46.83

O


ATOM
9179
O
HOH
W
269
42.839
15.510
2.646
1.00
50.23

O


ATOM
9180
O
HOH
W
270
53.273
−7.575
−8.127
1.00
59.58

O


ATOM
9181
O
HOH
W
271
32.659
16.190
−23.905
1.00
38.99

O


ATOM
9182
O
HOH
W
272
30.130
−12.373
8.961
1.00
56.13

O


ATOM
9183
O
HOH
W
273
38.784
13.668
−36.645
1.00
35.13

O


ATOM
9184
O
HOH
W
274
31.104
1.559
23.598
1.00
57.27

O


ATOM
9185
O
HOH
W
275
81.657
−36.807
−8.531
1.00
61.00

O


ATOM
9186
O
HOH
W
276
28.259
−9.123
17.061
1.00
66.33

O


ATOM
9187
O
HOH
W
277
74.640
10.010
−14.448
1.00
58.66

O


ATOM
9188
O
HOH
W
278
51.672
47.624
−28.267
1.00
83.54

O


ATOM
9189
O
HOH
W
279
31.801
14.035
−22.697
1.00
56.21

O


ATOM
9190
O
HOH
W
280
48.965
−56.394
1.074
1.00
52.99

O


ATOM
9191
O
HOH
W
281
35.984
12.918
7.155
1.00
73.87

O


ATOM
9192
O
HOH
W
282
39.220
24.103
−15.411
1.00
56.10

O


ATOM
9193
O
HOH
W
283
82.513
24.091
−23.548
1.00
46.49

O


ATOM
9194
O
HOH
W
284
71.235
−14.567
−9.579
1.00
63.27

O


ATOM
9195
O
HOH
W
285
40.489
24.887
−39.134
1.00
41.29

O


ATOM
9196
O
HOH
W
286
31.217
5.552
−3.887
1.00
54.56

O


ATOM
9197
O
HOH
W
287
43.073
−12.113
14.437
1.00
67.38

O


ATOM
9198
O
HOH
W
288
41.797
−9.307
15.285
1.00
77.90

O


ATOM
9199
O
HOH
W
289
81.627
−42.055
−15.117
1.00
54.24

O


ATOM
9200
O
HOH
W
290
62.244
−32.737
−7.755
1.00
40.89

O


ATOM
9201
O
HOH
W
291
64.210
−29.885
−4.076
1.00
32.81

O


ATOM
9202
O
HOH
W
292
58.895
−35.573
−0.679
1.00
32.40

O


ATOM
9203
O
HOH
W
293
56.735
−34.166
−0.445
1.00
38.48

O


ATOM
9204
O
HOH
W
294
58.151
−30.292
0.958
1.00
47.10

O


ATOM
9205
O
HOH
W
295
55.143
−32.877
−2.640
1.00
40.38

O


ATOM
9206
O
HOH
W
296
53.725
−33.616
−4.826
1.00
30.71

O


ATOM
9207
O
HOH
W
297
53.204
−31.192
−1.960
1.00
27.95

O


ATOM
9208
O
HOH
W
298
64.773
−39.562
5.940
1.00
37.11

O


ATOM
9209
O
HOH
W
299
63.454
−40.346
3.719
1.00
33.48

O


ATOM
9210
O
HOH
W
300
70.929
−25.565
−3.552
1.00
30.91

O


ATOM
9211
O
HOH
W
301
69.251
−23.763
−4.761
1.00
28.53

O


ATOM
9212
O
HOH
W
302
68.454
−19.371
−2.053
1.00
30.58

O


ATOM
9213
O
HOH
W
303
67.364
−18.575
0.260
1.00
38.26

O


ATOM
9214
O
HOH
W
304
57.160
−34.364
−10.979
1.00
33.18

O


ATOM
9215
O
HOH
W
305
61.204
−57.454
4.268
1.00
75.63

O


ATOM
9216
O
HOH
W
306
56.730
−31.024
−3.793
1.00
48.17

O


ATOM
9217
O
HOH
W
307
47.415
−47.036
−5.422
1.00
57.71

O


ATOM
9218
O
HOH
W
308
46.209
−46.389
−3.069
1.00
47.74

O


ATOM
9219
O
HOH
W
309
45.713
−44.103
−2.107
1.00
41.00

O


ATOM
9220
O
HOH
W
310
43.110
−43.797
−2.091
1.00
33.44

O


ATOM
9221
O
HOH
W
311
42.141
−41.451
−2.487
1.00
40.62

O


ATOM
9222
O
HOH
W
312
43.871
−40.170
−4.616
1.00
45.61

O


ATOM
9223
O
HOH
W
313
50.240
−52.391
4.392
1.00
48.19

O


ATOM
9224
O
HOH
W
314
45.922
−53.351
−4.156
1.00
60.44

O


ATOM
9225
O
HOH
W
315
45.630
−50.143
−2.403
1.00
58.28

O


ATOM
9226
O
HOH
W
316
45.142
−50.949
0.350
1.00
45.00

O


ATOM
9227
O
HOH
W
317
45.313
−51.628
2.682
1.00
43.29

O


ATOM
9228
O
HOH
W
318
57.968
−35.760
5.378
1.00
30.72

O


ATOM
9229
O
HOH
W
319
40.306
−36.855
1.232
1.00
35.14

O


ATOM
9230
O
HOH
W
320
36.996
−35.972
6.611
1.00
51.01

O


ATOM
9231
O
HOH
W
321
35.419
−34.298
4.601
1.00
64.21

O


ATOM
9232
O
HOH
W
322
42.265
−41.053
7.654
1.00
35.63

O


ATOM
9233
O
HOH
W
323
42.932
−43.525
8.391
1.00
47.39

O


ATOM
9234
O
HOH
W
324
49.568
−41.121
13.413
1.00
38.52

O


ATOM
9235
O
HOH
W
325
51.888
−40.050
15.225
1.00
54.02

O


ATOM
9236
O
HOH
W
326
52.387
−40.621
17.982
1.00
51.86

O


ATOM
9237
O
HOH
W
327
47.514
−44.885
13.940
1.00
42.74

O


ATOM
9238
O
HOH
W
328
51.324
−35.459
12.667
1.00
45.33

O


ATOM
9239
O
HOH
W
329
53.700
−33.540
13.321
1.00
63.89

O


ATOM
9240
O
HOH
W
330
55.987
−31.141
13.723
1.00
65.46

O


ATOM
9241
O
HOH
W
331
58.756
−23.502
−0.119
1.00
27.18

O


ATOM
9242
O
HOH
W
332
58.087
−25.850
−1.205
1.00
32.88

O


ATOM
9243
O
HOH
W
333
61.110
−21.801
0.234
1.00
28.97

O


ATOM
9244
O
HOH
W
334
61.686
−20.886
2.780
1.00
36.67

O


ATOM
9245
O
HOH
W
335
73.897
−23.796
4.991
1.00
44.56

O


ATOM
9246
O
HOH
W
336
74.045
−21.212
6.006
1.00
54.18

O


ATOM
9247
O
HOH
W
337
76.577
−24.196
5.079
1.00
42.85

O


ATOM
9248
O
HOH
W
338
76.437
−37.129
−8.119
1.00
57.89

O


ATOM
9249
O
HOH
W
339
53.634
−3.371
7.101
1.00
53.10

O


ATOM
9250
O
HOH
W
340
54.187
−10.567
7.280
1.00
58.39

O


ATOM
9251
O
HOH
W
341
60.617
−18.738
10.723
1.00
58.51

O


ATOM
9252
O
HOH
W
342
56.086
−22.336
−5.812
1.00
46.26

O


ATOM
9253
O
HOH
W
343
55.844
−24.396
−7.238
1.00
47.67

O


ATOM
9254
O
HOH
W
344
55.734
−15.022
−10.656
1.00
37.65

O


ATOM
9255
O
HOH
W
345
32.227
3.493
16.889
1.00
41.69

O


ATOM
9256
O
HOH
W
346
32.995
6.036
17.187
1.00
38.26

O


ATOM
9257
O
HOH
W
347
43.855
−6.680
−15.301
1.00
31.82

O


ATOM
9258
O
HOH
W
348
52.777
−10.540
−22.744
1.00
57.84

O


ATOM
9259
O
HOH
W
349
32.973
−8.777
0.726
1.00
43.68

O


ATOM
9260
O
HOH
W
350
31.795
−7.152
−0.910
1.00
52.97

O


ATOM
9261
O
HOH
W
351
39.854
−12.449
0.769
1.00
29.30

O


ATOM
9262
O
HOH
W
352
41.632
−13.591
2.624
1.00
27.96

O


ATOM
9263
O
HOH
W
353
37.139
−14.688
12.920
1.00
35.82

O


ATOM
9264
O
HOH
W
354
38.976
−12.566
16.101
1.00
43.62

O


ATOM
9265
O
HOH
W
355
30.926
−25.033
15.928
1.00
38.28

O


ATOM
9266
O
HOH
W
356
32.642
−27.492
16.851
1.00
59.83

O


ATOM
9267
O
HOH
W
357
33.558
−28.347
14.344
1.00
58.12

O


ATOM
9268
O
HOH
W
358
35.977
−29.130
13.344
1.00
58.73

O


ATOM
9269
O
HOH
W
359
37.818
−27.617
8.908
1.00
58.44

O


ATOM
9270
O
HOH
W
360
34.483
−25.533
11.257
1.00
47.88

O


ATOM
9271
O
HOH
W
361
33.652
−23.329
9.812
1.00
38.74

O


ATOM
9272
O
HOH
W
362
34.737
−22.982
7.515
1.00
44.25

O


ATOM
9273
O
HOH
W
363
31.499
−17.415
9.894
1.00
44.12

O


ATOM
9274
O
HOH
W
364
51.399
−14.940
−14.522
1.00
53.24

O


ATOM
9275
O
HOH
W
365
45.855
−23.406
−12.555
1.00
48.01

O


ATOM
9276
O
HOH
W
366
46.195
−26.619
−2.424
1.00
35.47

O


ATOM
9277
O
HOH
W
367
48.585
−13.372
16.041
1.00
79.24

O


ATOM
9278
O
HOH
W
368
48.885
−16.067
17.071
1.00
56.25

O


ATOM
9279
O
HOH
W
369
46.367
−15.771
16.682
1.00
41.39

O


ATOM
9280
O
HOH
W
370
42.851
−37.419
9.601
1.00
43.78

O


ATOM
9281
O
HOH
W
371
43.598
−36.638
12.092
1.00
51.81

O


ATOM
9282
O
HOH
W
372
37.469
−38.201
−4.913
1.00
57.09

O


ATOM
9283
O
HOH
W
373
53.585
−34.115
−8.277
1.00
39.64

O


ATOM
9284
O
HOH
W
374
54.662
−34.020
−10.670
1.00
47.00

O


ATOM
9285
O
HOH
W
375
56.958
−28.402
−7.616
1.00
49.88

O


ATOM
9286
O
HOH
W
376
88.727
30.365
−5.469
1.00
42.14

O


ATOM
9287
O
HOH
W
377
92.020
32.548
−6.742
1.00
60.45

O


ATOM
9288
O
HOH
W
378
84.725
42.573
−7.609
1.00
40.48

O


ATOM
9289
O
HOH
W
379
85.252
41.889
−11.799
1.00
64.42

O


ATOM
9290
O
HOH
W
380
85.092
49.969
0.422
1.00
64.39

O


ATOM
9291
O
HOH
W
381
85.911
45.396
0.491
1.00
38.83

O


ATOM
9292
O
HOH
W
382
87.802
41.943
−1.434
1.00
36.71

O


ATOM
9293
O
HOH
W
383
89.519
40.344
0.174
1.00
32.44

O


ATOM
9294
O
HOH
W
384
84.286
49.819
−14.844
1.00
74.07

O


ATOM
9295
O
HOH
W
385
83.672
41.939
−14.940
1.00
39.80

O


ATOM
9296
O
HOH
W
386
83.295
38.973
−15.352
1.00
55.46

O


ATOM
9297
O
HOH
W
387
85.204
37.793
−17.488
1.00
58.96

O


ATOM
9298
O
HOH
W
388
72.365
37.973
−17.684
1.00
32.25

O


ATOM
9299
O
HOH
W
389
71.257
35.561
−18.476
1.00
40.70

O


ATOM
9300
O
HOH
W
390
71.807
32.311
−14.434
1.00
31.88

O


ATOM
9301
O
HOH
W
391
69.838
34.721
−14.719
1.00
45.42

O


ATOM
9302
O
HOH
W
392
70.340
35.612
−11.135
1.00
44.21

O


ATOM
9303
O
HOH
W
393
65.944
38.414
−7.631
1.00
38.17

O


ATOM
9304
O
HOH
W
394
77.200
26.681
−14.865
1.00
32.87

O


ATOM
9305
O
HOH
W
395
75.188
25.234
−13.739
1.00
31.92

O


ATOM
9306
O
HOH
W
396
73.371
21.193
−16.417
1.00
33.68

O


ATOM
9307
O
HOH
W
397
72.126
20.703
−18.786
1.00
38.26

O


ATOM
9308
O
HOH
W
398
73.634
42.793
−22.468
1.00
42.63

O


ATOM
9309
O
HOH
W
399
74.545
41.623
−24.513
1.00
42.65

O


ATOM
9310
O
HOH
W
400
60.949
41.087
−13.743
1.00
32.39

O


ATOM
9311
O
HOH
W
401
59.269
41.117
−15.968
1.00
33.16

O


ATOM
9312
O
HOH
W
402
59.573
38.606
−17.369
1.00
37.23

O


ATOM
9313
O
HOH
W
403
61.334
36.357
−16.624
1.00
34.12

O


ATOM
9314
O
HOH
W
404
65.431
37.913
−32.478
1.00
72.71

O


ATOM
9315
O
HOH
W
405
74.252
31.182
−33.075
1.00
54.89

O


ATOM
9316
O
HOH
W
406
66.162
34.417
−19.631
1.00
41.16

O


ATOM
9317
O
HOH
W
407
70.541
34.371
−26.179
1.00
49.35

O


ATOM
9318
O
HOH
W
408
72.160
36.584
−26.563
1.00
44.65

O


ATOM
9319
O
HOH
W
409
66.812
25.333
−18.755
1.00
30.19

O


ATOM
9320
O
HOH
W
410
86.491
35.390
−9.504
1.00
58.45

O


ATOM
9321
O
HOH
W
411
90.048
27.970
−11.850
1.00
44.02

O


ATOM
9322
O
HOH
W
412
62.171
10.320
−31.392
1.00
77.96

O


ATOM
9323
O
HOH
W
413
61.847
27.094
−12.737
1.00
54.30

O


ATOM
9324
O
HOH
W
414
47.375
17.474
−33.175
1.00
62.08

O


ATOM
9325
O
HOH
W
415
44.847
16.856
−34.410
1.00
66.00

O


ATOM
9326
O
HOH
W
416
42.338
16.500
−34.798
1.00
44.22

O


ATOM
9327
O
HOH
W
417
46.428
14.822
−3.242
1.00
31.09

O


ATOM
9328
O
HOH
W
418
44.072
21.336
−19.449
1.00
24.93

O


ATOM
9329
O
HOH
W
419
45.933
21.946
−21.305
1.00
25.92

O


ATOM
9330
O
HOH
W
420
36.477
19.634
−19.350
1.00
38.85

O


ATOM
9331
O
HOH
W
421
35.761
21.880
−22.296
1.00
54.28

O


ATOM
9332
O
HOH
W
422
38.598
24.261
−20.814
1.00
34.09

O


ATOM
9333
O
HOH
W
423
42.073
24.026
−31.807
1.00
32.62

O


ATOM
9334
O
HOH
W
424
43.629
21.308
−34.851
1.00
46.44

O


ATOM
9335
O
HOH
W
425
38.238
12.974
−39.130
1.00
43.81

O


ATOM
9336
O
HOH
W
426
39.118
35.646
−35.292
1.00
25.51

O


ATOM
9337
O
HOH
W
427
42.266
35.370
−30.119
1.00
37.45

O


ATOM
9338
O
HOH
W
428
40.813
33.402
−28.894
1.00
33.01

O


ATOM
9339
O
HOH
W
429
41.930
32.862
−26.596
1.00
32.89

O


ATOM
9340
O
HOH
W
430
45.994
36.920
−27.628
1.00
49.65

O


ATOM
9341
O
HOH
W
431
44.666
38.262
−32.062
1.00
50.17

O


ATOM
9342
O
HOH
W
432
44.748
36.296
−29.774
1.00
48.50

O


ATOM
9343
O
HOH
W
433
41.893
38.285
−33.098
1.00
59.37

O


ATOM
9344
O
HOH
W
434
41.389
37.610
−35.338
1.00
44.70

O


ATOM
9345
O
HOH
W
435
37.463
28.292
−28.977
1.00
35.01

O


ATOM
9346
O
HOH
W
436
37.525
31.016
−27.801
1.00
60.63

O


ATOM
9347
O
HOH
W
437
43.596
33.620
−22.636
1.00
43.67

O


ATOM
9348
O
HOH
W
438
42.528
32.919
−20.253
1.00
49.09

O


ATOM
9349
O
HOH
W
439
39.951
31.335
−21.011
1.00
58.00

O


ATOM
9350
O
HOH
W
440
48.590
40.251
−26.117
1.00
55.18

O


ATOM
9351
O
HOH
W
441
51.164
36.110
−17.925
1.00
49.35

O


ATOM
9352
O
HOH
W
442
53.569
33.635
−16.169
1.00
35.61

O


ATOM
9353
O
HOH
W
443
60.658
22.781
−34.989
1.00
62.99

O


ATOM
9354
O
HOH
W
444
67.055
28.403
−32.430
1.00
71.44

O


ATOM
9355
O
HOH
W
445
64.929
28.511
−31.436
1.00
65.11

O


ATOM
9356
O
HOH
W
446
31.261
2.328
−12.309
1.00
60.56

O


ATOM
9357
O
HOH
W
447
62.959
−32.696
−0.260
1.00
37.45

O


ATOM
9358
O
HOH
W
448
32.942
6.020
−16.822
1.00
50.50

O


ATOM
9359
O
HOH
W
449
39.358
−34.872
−0.178
1.00
40.87

O


ATOM
9360
O
HOH
W
450
49.185
17.331
−39.030
1.00
85.64

O


ATOM
9361
O
HOH
W
451
34.510
7.195
15.541
1.00
40.34

O


ATOM
9362
O
HOH
W
452
47.514
20.262
−33.130
1.00
70.32

O


ATOM
9363
O
HOH
W
453
55.771
22.486
−35.412
1.00
55.29

O


ATOM
9364
O
HOH
W
454
51.628
22.787
−35.224
1.00
43.15

O


ATOM
9365
O
HOH
W
455
70.247
−33.650
4.619
1.00
44.86

O


ATOM
9366
O
HOH
W
456
56.201
−31.455
16.228
1.00
70.20

O


ATOM
9367
O
HOH
W
457
53.402
−26.502
13.095
1.00
43.52

O


ATOM
9368
O
HOH
W
458
50.905
−29.097
13.293
1.00
51.50

O


ATOM
9369
O
HOH
W
459
58.406
−24.593
13.085
1.00
64.84

O


ATOM
9370
O
HOH
W
460
33.095
−1.824
−7.051
1.00
53.88

O


ATOM
9371
O
HOH
W
461
33.672
−5.051
−9.420
1.00
80.71

O


ATOM
9372
O
HOH
W
462
31.765
−1.950
−9.780
1.00
62.59

O


ATOM
9373
O
HOH
W
463
28.204
6.068
17.286
1.00
70.37

O


ATOM
9374
O
HOH
W
464
27.676
1.116
7.983
1.00
72.76

O


ATOM
9375
O
HOH
W
465
60.829
−28.277
−6.075
1.00
46.61

O


ATOM
9376
O
HOH
W
466
63.126
−27.902
−5.719
1.00
45.15

O


ATOM
9377
O
HOH
W
467
30.807
−10.665
7.011
1.00
54.47

O


ATOM
9378
O
HOH
W
468
52.783
−44.340
20.735
1.00
61.14

O


ATOM
9379
O
HOH
W
469
53.681
−46.710
20.274
1.00
63.66

O


ATOM
9380
O
HOH
W
470
47.228
−42.064
14.688
1.00
51.18

O


ATOM
9381
O
HOH
W
471
45.107
−40.588
13.641
1.00
70.66

O


ATOM
9382
O
HOH
W
472
42.635
−41.371
12.545
1.00
64.29

O


ATOM
9383
O
HOH
W
473
35.539
3.814
−2.340
1.00
37.60

O


ATOM
9384
O
HOH
W
474
73.492
25.649
6.524
1.00
67.76

O


ATOM
9385
O
HOH
W
475
76.443
30.265
−3.269
1.00
48.17

O


ATOM
9386
O
HOH
W
476
54.665
30.777
−42.169
1.00
51.03

O


ATOM
9387
O
HOH
W
477
62.072
−26.897
−29.645
1.00
60.54

O


ATOM
9388
O
HOH
W
478
83.395
−25.719
−18.311
1.00
61.12

O


ATOM
9389
O
HOH
W
479
69.296
−29.080
−15.067
1.00
52.55

O


ATOM
9390
O
HOH
W
480
68.450
−34.644
−18.846
1.00
41.82

O


ATOM
9391
O
HOH
W
481
68.844
−42.933
−18.854
1.00
46.36

O


ATOM
9392
O
HOH
W
482
74.779
−51.629
−18.945
1.00
82.33

O


ATOM
9393
O
HOH
W
483
61.814
−31.687
−3.538
1.00
39.24

O


ATOM
9394
O
HOH
W
484
60.409
−33.103
−0.222
1.00
45.78

O


ATOM
9395
O
HOH
W
485
58.893
−27.935
0.472
1.00
37.41

O


ATOM
9396
O
HOH
W
486
64.559
−37.400
7.582
1.00
36.43

O


ATOM
9397
O
HOH
W
487
59.482
−60.439
1.522
1.00
74.62

O


ATOM
9398
O
HOH
W
488
68.935
−48.617
6.285
1.00
57.38

O


ATOM
9399
O
HOH
W
489
45.518
−41.693
6.827
1.00
48.99

O


ATOM
9400
O
HOH
W
490
73.965
−30.043
10.858
1.00
57.30

O


ATOM
9401
O
HOH
W
491
60.705
−18.803
3.885
1.00
48.85

O


ATOM
9402
O
HOH
W
492
58.316
−21.884
−13.444
1.00
54.51

O


ATOM
9403
O
HOH
W
493
51.463
−2.633
−7.386
1.00
35.03

O


ATOM
9404
O
HOH
W
494
33.079
−3.931
24.409
1.00
63.38

O


ATOM
9405
O
HOH
W
495
27.735
−22.892
14.172
1.00
53.70

O


ATOM
9406
O
HOH
W
496
29.069
−16.433
10.323
1.00
66.96

O


ATOM
9407
O
HOH
W
497
38.888
−20.098
−19.891
1.00
65.74

O


ATOM
9408
O
HOH
W
498
46.039
−37.082
13.291
1.00
56.96

O


ATOM
9409
O
HOH
W
499
90.450
25.023
−8.885
1.00
62.54

O


ATOM
9410
O
HOH
W
500
87.188
44.513
−1.400
1.00
60.58

O


ATOM
9411
O
HOH
W
501
88.088
41.534
−3.683
1.00
51.73

O


ATOM
9412
O
HOH
W
502
84.472
43.474
−10.213
1.00
58.23

O


ATOM
9413
O
HOH
W
503
60.288
45.674
−27.170
1.00
52.90

O


ATOM
9414
O
HOH
W
504
60.503
38.801
−24.799
1.00
54.90

O


ATOM
9415
O
HOH
W
505
65.628
22.382
−28.961
1.00
55.27

O

## Claims

1. An isolated polypeptide variant having isoprene synthase activity, wherein the polypeptide variant comprises a substitution at residue X288 corresponding to SEQ ID NO:1, and wherein the polypeptide has increased protein solubility compared a parent polypeptide which does not comprise the substitution at residue X288, wherein said polypeptide variant is 90% identical to isoprene synthase polypeptide of SEQ ID NO: 1.

2. The polypeptide variant of claim 1 wherein the substitution is at S288.

3. The polypeptide variant of claim 2 wherein the substitution is S288C.

4. The polypeptide variant of claim 1, where the polypeptide has at least 5% to at least 75% increased protein solubility compared to the parent polypeptide.

5. The polypeptide variant of claim 1 wherein the polypeptide is derived from a parent polypeptide isolated from a plant.

6. The polypeptide variant of claim 5 wherein the parent polypeptide is isolated from a plant species selected from poplar (Populus sp.), kudzu (Pueraria sp.), English oak (Quercus sp.) or willow (Salix sp.).

7. The polypeptide variant of claim 6 wherein the species is Populus sp.

8. The polypeptide variant of claim 7 wherein the species is P. alba, P. tremuloides, P. trichocharpa, or P. nigra.

9. The polypeptide variant of claim 6 wherein the species is Pueraria sp.

10. The polypeptide variant of claim 9 wherein the species is Pueraria montana.

11. The polypeptide variant of claim 6 wherein the species is Quercus sp.

12. The polypeptide variant of claim 11 wherein the species is Quercus rubur.

13. The polypeptide variant of claim 6 wherein the species is Salix sp.

14. The polypeptide variant of claim 13 wherein the species is S. alba or S. baylonica.