# Patent

## Title
Isoprene synthase variants for improved production of isoprene

## Abstract

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved specific productivity. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims priority benefit of U.S. provisional patent application No. 61/407,415, filed on Oct. 27, 2010, the content of which is hereby incorporated by reference herein in its entirety.


FIELD OF THE INVENTION
The present invention provides methods and compositions comprising isoprene synthase variants. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.
BACKGROUND OF THE INVENTION
Isoprenoids are isoprene polymers that find use in pharmaceuticals, neutraceuticals, flavors, fragrances, and rubber products. Natural isoprenoid supplies, however, are limited due to ecological concerns. For this reason, and to provide isoprenoid compositions having fewer impurities and greater uniformity, isoprenoids such as rubber are often produced synthetically.
Isoprene(2-methyl-1,3-butadiene) is a volatile hydrocarbon that is insoluble in water and soluble in alcohol. Commercially viable quantities of isoprene can be obtained by direct isolation from petroleum C5 cracking fractions or by dehydration of C5 isoalkanes or isoalkenes (Weissermel and Arpe, Industrial Organic Chemistry, 4th ed., Wiley-VCH, pp. 117-122, 2003). The C5 skeleton can also be synthesized from smaller subunits. It would be desirable, however, to have a commercially viable method of producing isoprene that was independent of nonrenewable resources.
Biosynthetic production of isoprene occurs by two distinct metabolic pathways (Julsing et al., Appl Microbiol Biotechnol, 75:1377-1384, 2007). In eukaryotes and archae, isoprene is formed via the mevalonate (MVA) pathway, while some eubacteria and higher plants produce isoprene via the methylerythritol phosphate (MEP) pathway. Isoprene emissions from plants are light and temperature-dependent with increases linked to leaf development. An isoprene-producing enzyme, isoprene synthase, has been identified in Aspen trees (Silver and Fall, Plant Physiol, 97:1588-1591, 1991; and Silver and Fall, J Biol Chem, 270:13010-13016, 1995) and is believed to be responsible for the in vivo production of isoprene from whole leaves. Bacterial production of isoprene has also been described (Kuzma et al., Curr Microbiol, 30:97-103, 1995; and Wilkins, Chemosphere, 32:1427-1434, 1996), and varies in amount with the phase of bacterial growth and the nutrient content of the culture medium (U.S. Pat. No. 5,849,970 to Fall et al.; and Wagner et al., J Bacteriol, 181:4700-4703, 1999). The levels of isoprene obtainable through bacterial systems of the prior art, however, are insufficient for commercial uses.
Thus what the art needs is an efficient, large scale isoprene biological production process to provide feedstock for the manufacture of isoprenoids.
All patents, patent applications, articles and publications mentioned herein are hereby expressly incorporated herein by reference.
SUMMARY OF THE INVENTION
The present invention provides compositions comprising at least one isoprene synthase polypeptide variant and method of making and using such variants. The variant comprises one or more amino acid residue substitution(s) from a parent isoprene synthase polypeptide, wherein the parent isoprene synthase may be a wild type or non-wild type sequence. The invention provides amino acid residue substitutions at particular positions within the polypeptide, wherein the substitution may result in at least one improved property as compared to its parent sequence. In some particularly preferred embodiments, the at least one improved property is selected from but not limited to the group consisting of: specific productivity, yield, cellular performance index, specific activity, growth of the host cell in which the polypeptide variant is expressed, expression, stability and solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.
In one aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises one or more substitutions(s) at one or more residue(s) in N-terminus region, surface loop, surface, active site, dimer interface, substrate capture loop, or in a buried region and wherein the polypeptide has at least 30% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1. In some embodiments, the substitution in the N-terminus region is at a residue selected from the group consisting of X2, X22, X36, X43, and X58. In some embodiments, the substitution is at a residue selected from the group consisting of E2, S22, K36, R43, and E58. In some embodiments, wherein the substitution is selected from the group consisting of E2V, S22K, S22R, K36D, K36E, K36H, K36W, R43E, and E58F. In some embodiments, the substitution in the buried region is at a residue selected from the group consisting of X71, X89, X118, X161, X228, X268, X282, X288, X331, X391, X392, X437, X460, X461, X481, X488, and X502. In some embodiments, the substitution is at a residue selected from the group consisting of R71, F89, A118, K161, M228, V268, S282, S288, C331, A391, W392, C437, M460, R461, T481, E488, and T502. In some embodiments, the substitution is selected from the group consisting of R71I, F89D, F89E, A118E, A118P, K161C, M228Y, V268I, S282H, S282W, S288A, S288T, S288Y, C331P, A391G, W392C, W392F, W392M, W392S, W392V, W392Y, C437L, C437M, M460A, R461A, R461Y, T481Y, E488L, T502F and T502M. In some embodiments, the substitution in the surface loop is at a residue selected from the group consisting of X120, X151, X153, X254, X380, and X409. In some embodiments, the substitution is at a residue selected from the group consisting of S120, L151, H254, S380, and V409. In some embodiments, the substitution is selected from the group consisting of S120M, S120Q, L151F, L151Y, G153P, H254C, S380E, and V409T. In some embodiments, the substitution in the dimer interface is at X247. In some embodiments, wherein the substitution is at V247. In some embodiments, the substitution is V247M. In some embodiments, the substitution on the surface is at a residue selected from the group consisting of X348, X376, and X389. In some embodiments, the substitution is at a residue selected from the group consisting of K348, L376, and G389. In some embodiments, the substitution is selected from the group consisting of K348Y, and G389D. In some embodiments, the substitution in the substrate capture loop is at a residue selected from the group consisting of X443, X444, X447 and X448. In some embodiments, the substitution is at a residue selected from the group consisting of S444, I447 and A448. In some embodiments, the substitution is selected from the group consisting of S444S, I447T and A448V.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises the following immutable residues with residue numbering corresponding to SEQ ID NO:1: X4, X9, X243, X258, X259, X262, X266, X280, X294, X295, X298, X305, X387, X396, X397, X435, X439, X446, X449, X450, X514, and X518, and wherein the polypeptide further comprises one or more substitutions(s) at residues selected from the group consisting of: X2, X22, X36, X43, X58, X71, X89, X118, X120, X151, X153, X161, X228, X234, X247, X254, X268, X282, X288, X331, X348, X376, X380, X389, X391, X392, X409, X437, X443, X444, X447, X448, X460, X461, X481, X488, and X502, and wherein the polypeptide has at least 30% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1. In some embodiments, the substitutions are at residues selected from the group consisting of: E2, S22, K36, R43, E58, R71, F89, A118, S120, L151, K161, M228, Q234, V247, H254, V268, S282, S288, C331, K348, L376, S380, G389, A391, W392, V409, C437, S444, I447, A448, M460, R461, T481, E488, and T502. In some embodiments, the substitution(s) are selected from the group consisting of: E2V, S22K, S22R, K36D, K36E, K36H, K36W, R43E, E58F, R71I, F89D, F89E, A118E, A118P, S120M, S120Q, L151F, L151Y, G153P, K161C, M228Y, Q234R, V247I, V247L, V247M, H254C, V268I, S282H, S282W, S288A, S288T, S288Y, C331P, K348Y, S380E, G389D, A391G, W392C, W392F, W392M, W392S, W392V, W392Y, V409T, C437L, C437M, S444D, S444E, I447T, I447V, A448V, M460A, R461A, T481Y, E488L, T502F and T502M.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises the following immutable residues with residue numbering corresponding to SEQ ID NO:1: X4, X9, X243, X258, X259, X262, X266, X280, X294, X295, X298, X305, X387, X396, X397, X435, X439, X446, X449, X450, X514, and X518, and wherein the polypeptide further comprises one or more substitutions(s) at residues selected from the group consisting of: X134, X138, X143, X156, X159, X163, X166, X167, X170, X414, X421, and X491 and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of K134, K138, L143, I156, E159, F163, S166, H167, E170, K414, and Q421. In some embodiments, the substitution is selected from the group consisting of K134P, K138C, L143F, L143V, I156G, E159G, E159Q, F163C, F163E, F163Q, F163V, F163Y, S166C, S166D, S166G, S166P, S166V, H167M, E170G, E170H, E170K, E170N, E170R, E170S, E170W, K414F, K414G, K414N, K414P, and Q421R.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises the following immutable residues with residue numbering corresponding to SEQ ID NO:1: X4, X9, X243, X258, X259, X262, X266, X280, X294, X295, X298, X305, X387, X396, X397, X435, X439, X446, X449, X450, X514, and X518, and wherein the polypeptide further comprises one or more substitutions(s) at residues selected from the group consisting of: X29, X47, X86, X94, X131, X134, X156, X162, X169, X178, X179, X231, X242, X369, X414, and X421 and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of E29, N47, S86, K94, E131, K134, I156, V162, K169, K178, E179, S231, R242, F369, K414, and Q421. In some embodiments, the substitution is selected from the group consisting of E29N, N47V, S86C, K94A, E131F, K134E, K134P, I156G, V162P, K169C, K178E, E179T, S231D, S231K, S231R, S231T, S231V, R242N, R242I, F369C, K414C, K414F, K414G, K414N, and Q421D.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises the following immutable residues with residue numbering corresponding to SEQ ID NO:1: X4, X9, X243, X258, X259, X262, X266, X280, X294, X295, X298, X305, X387, X396, X397, X435, X439, X446, X449, X450, X514, and X518, and wherein the polypeptide further comprises one or more substitutions(s) at residues selected from the group consisting of: X30, X84, X134, X140, X143, X163, X166, X169, X170 and X172 and wherein the polypeptide has at least 20% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1 and at least 20% more growth in a host cell expressing the polypeptide compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of V84, K134, I140, L143, F163, S166, K169, E170 and S172. In some embodiments, the substitution is selected from the group consisting of V30K, V84T, K134C, K134D, K134E, I140S, I140T, L143F, L143I, L143M, L143V, F163I, F163M, S166P, S166V, K169Q, E170H, E170K, and S172V.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises one or more substitution(s) in: (i) the N-terminus, (ii) the N-terminal helix region from residues 134 to 179 wherein the residue numbering corresponds to SEQ ID NO:1 (wild type MEA isoprene synthase) or (iii) at other residues outside of the N-terminal helix region that interact with the N-terminal helix region, and wherein the polypeptide has at least 20% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1. In some embodiments, the substitutions are at residues selected from the group consisting of: X30, X84, X134, X140, X143, X163, X166, X169, X170 and X172. In some embodiments, the substitutions are at residues selected from the group consisting of: V84, K134, I140, L143, F163, S166, K169, E170 and S172. In some embodiments, the substitutions are selected from the group consisting of: V30K, V84T, K134C, K134D, K134E, I140S, I140T, L143F, L143I, L143M, L143V, F163I, F163M, S166P, S166V, K169Q, E170H, E170K, and S172V.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises one or more substitutions(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X2, X22, X36, X43, X58, X71, X89, X118, X120, X151, X153, X161, X228, X234, X247, X254, X268, X282, X288, X331, X348, X376, X380, X389, X391, X392, X409, X437, X443, X444, X447, X448, X460, X461, X481, X488, and X502, and wherein the polypeptide has at least 30% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1. In some embodiments, the substitution is at a residue selected from the group consisting of E2, S22, K36, R43, E58, R71, F89, A118, S120, L151, K161, M228, Q234, V247, H254, V268, S282, S288, C331, K348, L376, S380, G389, A391, W392, V409, C437, S444, I447, A448, M460, R461, T481, E488, and T502. In some embodiments, the substitution is selected from the group consisting of E2V, S22K, S22R, K36D, K36E, K36H, K36W, R43E, E58F, R71I, F89D, F89E, A118E, A118P, S120M, S120Q, L151F, L151Y, G153P, K161C, M228Y, Q234R, V247I, V247L, V247M, H254C, V268I, S282H, S282W, S288A, S288T, S288Y, C331P, K348Y, S380E, G389D, A391G, W392C, W392F, W392M, W392S, W392V, W392Y, V409T, C437L, C437M, S444D, S444E, I447T, I447V, A448V, M460A, R461A, T481Y, E488L, T502F and T502M.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises one or more substitutions(s) at one or more residue(s) in N-terminus region, N-terminal helix region, surface loop, surface, active site, dimer interface, substrate capture loop, or in a buried region and wherein the polypeptide has at least 40% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1. In some embodiments, the substitution in the N-terminus region is at a residue selected from the group consisting of: X18, X36, and X82. In some embodiments, the substitution is at a residue selected from the group consisting of Y18, K36, and R82. In some embodiments, the substitution is selected from the group consisting of: Y18E, Y18D, Y18S, K36P, and R82Q. In some embodiments, the substitution in the N-terminus helix region is at a residue selected from the group consisting of: X137, X143, X163, and X170. In some embodiments, the substitution is at a residue selected from the group consisting of: I137, L143, F163, and E170. In some embodiments, the substitution is selected from the group consisting of: I137C, L143N, F163I, F163Q, and E170G. In some embodiments, the substitution in the surface loop is at a residue selected from the group consisting of: X87, X409, and X542. In some embodiments, the substitution is at a residue selected from the group consisting of: G87, V409, and F542. In some embodiments, the substitution is selected from the group consisting of: G87S, G87N, G87R, V409S, and F542N. In some embodiments, the substitution in the surface is at residue X251. In some embodiments, the substitution is at residue T251. In some embodiments, the substitution is T251E. In some embodiments, the substitution in the dimer interface is at residue X242. In some embodiments, the substitution is at residue R242. In some embodiments, the substitution is R242T. In some embodiments, the substitution in the buried region is at a residue selected from the group consisting of: X437, X460, and X461. In some embodiments, the substitution is at a residue selected from the group consisting of: C437, M460, and R461. In some embodiments, the substitution is selected from the group consisting of: C437M, C437K, M460Q, M460G, M460A, R461D, R461S, R461T, and R461. In some embodiments, the substitution in the substrate capture loop is at a residue selected from the group consisting of: X443, X444, and X447. In some embodiments, the substitution is at a residue selected from the group consisting of: S444, and I447. In some embodiments, the substitution is selected from the group consisting of: S444P, I447Q, I447T, I447M, I447E, I447S, and I447R.
In another aspect, the invention provides for isolated polypeptides having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide comprises one or more substitutions(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X134, X138, X143, X156, X159, X163, X166, X167, X170, X414, X421, and X491 and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of K134, K138, L143, I156, E159, F163, S166, H167, E170, K414, and Q421. In some embodiments, the substitution is selected from the group consisting of K134P, K138C, L143F, L143V, I156G, E159G, E159Q, F163C, F163E, F163Q, F163V, F163Y, S166C, S166D, S166G, S166P, S166V, H167M, E170G, E170H, E170K, E170N, E170R, E170S, E170W, K414F, K414G, K414N, K414P, and Q421R.
In another aspect, the invention provides for isolated polypeptides having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide comprises one or more substitutions(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X29, X47, X86, X94, X131, X134, X156, X162, X169, X178, X179, X231, X242, X369, X414, and X421 and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of E29, N47, S86, K94, E131, K134, I156, V162, K169, K178, E179, S231, R242, F369, K414, and Q421. In some embodiments, the substitution is selected from the group consisting of E29N, N47V, S86C, K94A, E131F, K134E, K134P, I156G, V162P, K169C, K178E, E179T, S231D, S231K, S231R, S231T, S231V, R242N, R242I, F369C, K414C, K414F, K414G, K414N, and Q421D.
In another aspect, the invention provides for isolated polypeptides having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide comprises one or more substitutions(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X50, X81, X134, X137, X143, X156, X159, X166, X167, X169, X170, and X414 and wherein a host cell expressing the polypeptide has at least 30% more growth compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of K50, D81, K134, I137, L143, I156, E159, S166, H167, K169, E170, and K414. In some embodiments, the substitution is selected from the group consisting of K50S, D81F, K134E, K134P, I137N, L143V, I156G, E159D, E159G, E159Q, S166C, S166W, H167M, H167N, K169C, E170H, E170K, E170W, K414C, K414F, K414G, K414N, and K414P.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide comprises one or more substitutions(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X30, X84, X134, X140, X143, X163, X166, X169, X170 and X172 and wherein the polypeptide has at least 20% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1 and at least 20% more growth in a host cell expressing the polypeptide compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of V84, K134, I140, L143, F163, S166, K169, E170 and S172. In some embodiments, the substitutions are selected from the group consisting of: V30K, V84T, K134C, K134D, K134E, I140S, I140T, L143F, L143I, L143M, L143V, F163I, F163M, S166P, S166V, K169Q, E170H, E170K, and S172V.
In another aspect, the invention provides for isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises two or more substitution(s) residues corresponding to SEQ ID NO:1 selected from the group consisting of: X22, X71, X87, X162, X242, X288, X409, X414, X443, X444, X460, and X502 wherein the residue numbering corresponds to SEQ ID NO:1, and wherein the polypeptide has at least 160% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1. In some embodiments, the substitution is at a residue selected from the group consisting of S22, R71, G87, V162, R242, S288, V409, K414, S444, M460, and T502. In some embodiments, the substitutions are selected from the group consisting of S22R, R71I or R, G87R, V162P, R242N, S288C, V409T, KX414F, S444D, M460A, and T502M. In some embodiments, the substitution is selected from the group consisting of: a) S22R, R71I, S288C, S444D, M460A, and T502M; b) G87R, V162P, R242N, S288C, V409T, K414R, and S444D; or c) G87R, V162P, R242N, S288C, V409T, and K414F.
In another aspect, the invention provides for isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises two or more substitution(s) residues corresponding to SEQ ID NO:1 selected from the group consisting of: X47, X87, X156, X162, X170, X231, X242, X288, X409, X414, and X447 wherein the residue numbering corresponds to SEQ ID NO:1, and wherein the polypeptide has at least 30% increase in specific activity of isoprene synthase as compared to wild-type isoprene synthase of SEQ ID NO:1 and at least 30% more growth when the polypeptide is expressed in a host cell, as compared to wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitutions are at residues selected from the group consisting of N47, G87, I156, V162, E170, S231, R242, S288, V409, K414, and I447. In some embodiments, the substitutions are selected from the group consisting of N47V, G87R, I156G, V162P, E170H, S231T, R242N, S288C, S288T, V409T, and K414F. In some embodiments, the substitutions are selected from the group consisting of N47V, I156G, E170H, S231T, S288T, and K414F.
In another aspect, the invention provides for isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises one or more substitution(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X2, X18, X19, X21, X24, X26, X27, X29, X37, X42, X47, X48, X49, X56, X81, X82, X84, X93, X94, X95, X120, X123, X126, X131, X132, X134, X137, X139, X143, X151, X155, X166, X167, X169, X170, X171, X175, X179, X180, X197, X229, X231, X240, X242, X245, X246, X247, X251, X271, X282, X306, X317, X319, X369, X371, X376, X379, X380, X389, X392, X393, X408, X409, X421, X422, X423, X429, X437, X443, X444, X447, X455, X458, X461, X464, X466, X470, X473, X500, X502, X506, X513, X525 and, X531 and wherein the polypeptide has (a) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.9 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.0; and (b) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.8 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.2; and (c) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.5 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.5.
In some embodiments, the substitution is at a residue selected from the group consisting of E2, Y18, L19, S21, T24, E26, S27, E29, K37, V42, N47, N48, E49, L56, D81, R82, V84, T93, K94, T95, S120, K123, N126, E131, N132, K134, I137, A139, L143, L151, N155, S166, H167, K169, E170, L171, K175, E179, L180, Q197, I229, S231, T240, R242, R245, R246, V247, T251, A271, S282, L306, D317, N319, F369, Q371, L376, K379, S380, G389, W392, K393, V408, V409, Q421, K422, Y423, R429, C437, S444, I447, S455, C458, R461, G464, S466, A470, S473, V500, T502, L506, T513, E525 and, V531. In some embodiments, the substitution is selected from the group consisting of E2A or K or P, Y18D or E or K or S, L19Y, S21W, T24L or V, E26C, S27D or N, E29N, K37C or D or P or Q or S, V42M, N47D or S, N48D or G or T, E49L or V, L56E or F or G or I or K or T or V or Y, D81Q, R82N or T or V or Y, V84M, T93C or F or R or S, K94G or P, T95D or F or G or I or N or W, S120C or G or M or Q, K123V, N126E, E131H or K or L or M or T or W or Y, N132I or P, K134A, I137T, A139C or Q, L143C or D or E or H or K or M or Q or T or V or Y, L151A or F, N155A or C or G or H or Q or R or S or W, S166N, H167F or I or N or Q or V, K169A or C or H or N or Q or V, E170L or S or W or Y, L171A or N or Q or T or V or Y, K175C or F or I or Q or R, E179D, L180A or I, Q197C or D or N, I229C, S231A, T240C, R242G, R245C or K or M or Q or T or V, R246N, V247L or M, T251D or E or N or P or Q or S, A271T, S282Y, L306C, D317N, N319M, F369C or D or E or G or S, Q371F, L376I or M, K379G or Q, S380E, G389A or D or E or K or N or Q or S or V, W392Y, K393C or I or T or V, V408T, V409T, Q421H, K422D, Y423N or S, R429E or F or Q, C437M, S444D or E, I447T, S455A, C458T, R461A, G464C or M or N or Q or S, S466D, A470I or L, S473I, V500A or C, T502M, L506M, T513C or G or K or N, E525F or R, V531E or H or K or Q or R or S.
In another aspect, the invention provides for isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises one or more substitution(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X2, X6, X18, X20, X22, X23, X24, X25, X26, X27, X28, X29, X30, X31, X32, X36, X37, X42, X44, X47, X48, X49, X50, X53, X54, X55, X56, X58, X59, X68, X71, X74, X77, X78, X79, X81, X82, X83, X84, X86, X87, X91, X93, X94, X95, X97, X98, X99, X109, X115, X116, X117, X118, X120, X123, X125, X126, X127, X128, X130, X131, X132, X133, X134, X136, X137, X138, X139, X140, X143, X151, X153, X155, X156, X159, X160, X161, X162, X163, X164, X166, X167, X169, X170, X171, X172, X175, X176, X177, X178, X179, X180, X181, X182, X190, X194, X197, X204, X211, X215, X217, X219, X221, X228, X229, X231, X232, X235, X241, X242, X245, X246, X247, X251, X254, X271, X272, X278, X279, X282, X296, X302, X317, X319, X320, X327, X331, X348, X351, X357, X361, X364, X365, X368, X369, X370, X371, X373, X377, X380, X383, X386, X389, X392, X393, X407, X408, X409, X410, X411, X414, X422, X423, X424, X428, X429, X432, X436, X437, X440, X443, X444, X447, X448, X457, X460, X461, X462, X463, X464, X465, X466, X468, X470, X471, X472, X473, X475, X480, X490, X491, X492, X494, X496, X500, X501, X502, X503, X510, X513, X515, X519, X525, X531, X536, X537, X540, X541, X542, and X544 and wherein the polypeptide has (a) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.9 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.0; and (b) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.8 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.2. In some embodiments, wherein the substitution is at a residue selected from the group consisting of E2, S6, Y18, L20, S22, D23, T24, D25, E26, S27, I28, E29, Y31, K32, K36, K37, V42, R44, N47, N48, E49, K50, F53, L54, T55, L56, E58, L59, L68, R71, S74, R77, G78, A79, D81, R82, F83, V84, S86, G87, A91, T93, K94, T95, L97, H98, G99, Q109, S115, Q116, E117, A118, S120, K123, Q125, N126, G127, N128, L130, E131, N132, L133, K134, D136, I137, K138, A139, I140, L143, L151, N155, I156, E159, A160, K161, V162, F163, A164, S166, H167, K169, E170, L171, S172, K175, I176, G177, K178, E179, L180, A181, E182, L190, R194, Q197, S204, K211, N215, V217, L219, L221, M228, I229, S231, V232, R235, S241, R242, R245, R246, V247, T251, H254, A271, F272, D278, C279, S282, I296, T302, D317, N319, A320, Y327, C331, K348, G351, Y357, A361, D364, L365, A368, F369, L370, Q371, A373, Y377, S380, T383, D386, G389, W392, K393, A407, V408, V409, Q410, N411, K414, K422, Y423, H424, S428, R429, H432, L436, C437, L440, S444, I447, A448, S457, M460, R461, T462, K463, G464, I465, S466, E468, A470, T471, E472, S473, M475, E480, L490, G492, L494, A496, V500, E501, T502, A503, S510, T513, H515, A519, E525, V531, T536, E537, L540, P541, F542, and R544. In some embodiments, the substitution is at a residue selected from the group consisting of E2C or D or N or T or V, S6N or T, Y18A or Q or R, L20T, S22Q, D23N, T24C, D25T, E26D or H or K or M or R or S or V, S27A or C or G or H or I or L or M or P or Q, I28D or N, E29Q, V30A or D or E or M or R or T, Y31N, K32E, K36A or C or D or E or M or N or P or Q, K37A or E or G or H or M or N or R or T, V42F or I, R44N or Q, N47A or G or H or M or Q or T or W, N48H or I or K, E49A or C, K50A or D or E or F or H or S or Y, F53E or H or N or P or Q or V, L54M, T55C or D or E, L56C or N, E58N, L59H or T, L68I, R71K or M, S74D or E or N or Y, R77L, G78A or D or F or L or M, A79Q or T, D81A or F or G or M or R or S or T or V, R82A or E or H or I or K or M or Q or S, F83W, V84A, S86A or D or M, G87D or P, A91K or W, T93A or D or E or G or L or N or P or Y, K94A or D or E or H or I or L or M or N or R or S or T, T95A or E or P or Q or S or V or Y, L97F, H98A or D or F or G or I or L or M or N or Q, G99E or F or M, Q109E, S115A, Q116A or C or D or E or I or P, E117C or F or L or M or V, A118M, S120H or T or V, K123L or T, Q125E or I or Y, N126A or C or D or M or T or V, G127C, N128C or D or P or Q, L130E, E131A or C or P or Q or S or V, N132C or D or F or H or L or R or W or Y, L133D, K134E or M or Q or S or T or V, D136E, I137E or H or N, K138I or N, A139N, I140M or W, L143S, L151C or H or I, G153C, N155I or T or V or Y, I156D or N or T, E159M, A160I, K161A or C or N or Q, V162S, F163E or Q, A164T, S166A or D or G, H167A or E or G or K or M or R or S or T or W, K169D or I or M or S or T, E170H or K or M or Q or T or V, L171H or K or R or S, S172A or C, K175S, I176M, G177A or C, K178A or F or R or S or T, E179A or C or L or M or N, L180C or Q or T, A181H or Q or S or V, E182S, L190I or M, R194L, Q197S, S204C, K211A or N or Q, N215C or H, V217I, L219C, L221M, M228F or Y, I229V, S231K or Q or T, V232I, R235K, S241A or M or T, R242A or D or E or H or I or M or N or Q or S or T, R245I or L, R246D or K, V247T, T251A or G or K or R, H254D, A271C or V, F272D or G or P or W, D278A or E or N or Q or S or T or V or W, C279A, S282A or Q, I296V, T302H, D317E or Q, N319F, A320C, Y327M, C331P, K348R or Y, G351D or N, Y357M, A361T, D364E or V, L365C or M, A368N, F369M or N or R or T or V, L370G or Q, Q371C or S, A373G, Y377W, S380A or C or D or Q or T or V, T383S, D386E or N, G389H or I, W392I or S or T or V, K393Q, A407G, V408I, V409H or I, Q410C or D or K or L or M or T, N411G, K414E or G or L or N or P, K422A or N or T, Y423Q, H424E or P or Q or V, S428E or Q, R429I or L or T or W or Y, H432E, L436M or Y, C437K or T, L440I, S444P, I447A or E or M or Q or S, A448E or M or N or P or Q or V, S457N or T, M460Q or R or S, R461D or E or G or Q or S or T, T462Q, K463A or D or E, G464L or R, I465A or C or G or S or T, S466P, E468D, A470M, T471E or H or Q, E472D or S, S473L or V, M475T, E480N, L490A or D or E or F or H or M, G492C, L494D, A496P or T, V500L or M, E501D, T502A or C or R or V, A503I, S510C or V, T513V, H515N, A519S or T, E525A or C or P or Q or S, V531A or M or T, T536A or F or G, E537K or T, L540A or P, P541M, F542P, and R544C.
In another aspect, the invention provides for isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises one or more substitution(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X2, X3, X13, X17, X18, X19, X20, X23, X25, X26, X27, X28, X29, X30, X31, X32, X33, X34, X36, X37, X40, X41, X42, X43, X44, X45, X46, X47, X48, X49, X50, X51, X53, X54, X55, X56, X57, X59, X60, X62, X71, X73, X74, X75, X77, X78, X79, X81, X82, X83, X84, X85, X86, X87, X88, X89, X91, X92, X93, X94, X95, X97, X98, X99, X100, X101, X102, X103, X107, X109, X111, X113, X114, X115, X116, X117, X118, X119, X120, X121, X123, X124, X125, X127, X128, X129, X130, X131, X133, X134, X135, X136, X137, X138, X139, X140, X143, X146, X151, X152, X153, X155, X156, X158, X160, X161, X162, X163, X166, X167, X169, X170, X171, X172, X175, X176, X177, X178, X179, X180, X181, X182, X183, X185, X187, X193, X194, X196, X197, X204, X210, X211, X212, X215, X216, X217, X218, X219, X220, X222, X223, X224, X226, X228, X229, X231, X232, X235, X240, X241, X242, X246, X251, X253, X260, X268, X270, X271, X272, X275, X276, X278, X282, X307, X314, X315, X317, X320, X321, X323, X328, X329, X331, X332, X333, X343, X345, X346, X350, X351, X352, X356, X357, X360, X361, X363, X364, X366, X367, X368, X369, X370, X371, X378, X379, X380, X383, X386, X389, X390, X392, X393, X402, X405, X408, X409, X410, X413, X414, X418, X422, X423, X424, X425, X426, X428, X429, X431, X432, X437, X444, X447, X448, X457, X460, X461, X462, X463, X464, X466, X467, X468, X469, X471, X472, X475, X484, X489, X490, X491, X492, X493, X494, X497, X500, X501, X502, X503, X504, X506, X509, X510, X511, X513, X515, X517, X519, X522, X528, X529, X531, X534, X535, X536, X537, X539, X540, X542, and X544 and wherein the polypeptide has (a) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.9 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.0; or (b) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.8 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.2 and (c) minimum performance indices (PI) relative to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.5 and where at least one PI relative to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.5. In some embodiments, the substitution is at a residue selected from the group consisting of E2, A3, S13, D17, Y18, L19, L20, D23, D25, E26, S27, I28, E29, Y31, K32, D33, K34, K36, K37, A40, E41, V42, R43, R44, E45, I46, N47, N48, E49, K50, A51, F53, L54, T55, L56, L57, L59, I60, N62, R71, E73, S74, D75, R77, G78, A79, D81, R82, F83, V84, S85, S86, G87, G88, F89, A91, V92, T93, K94, T95, L97, H98, G99, T100, A101, L102, S103, L107, Q109, G111, E113, V114, S115, Q116, E117, A118, F119, S120, G121, K123, D124, Q125, G127, N128, F129, L130, E131, L133, K134, E135, D136, I137, K138, A139, I140, L143, A146, L151, E152, N155, I156, D158, A160, K161, V162, F163, S166, H167, K169, E170, L171, S172, K175, I176, G177, K178, E179, L180, A181, E182, Q183, N185, A187, H193, R194, T196, Q197, S204, K210, K211, E212, N215, Q216, V217, L218, L219, E220, A222, I223, L224, Y226, M228, I229, S231, V232, R235, T240, S241, R242, R246, T251, L253, L260, V268, V270, A271, F272, Q275, Y276, D278, S282, E307, E314, R315, D317, A320, I321, D323, M328, K329, C331, F332, L333, A343, D345, N346, K350, G351, E352, P356, Y357, K360, A361, A363, D364, C366, N367, A368, F369, L370, Q371, N378, K379, S380, T383, D386, G389, N390, W392, K393, V402, Y405, V408, V409, Q410, K413, K414, E418, K422, Y423, H424, D425, T426, S428, R429, S431, H432, C437, S444, I447, A448, S457, M460, R461, T462, K463, G464, S466, E467, E468, L469, T471, E472, M475, K484, K489, L490, G492, S493, L494, K497, V500, E501, T502, A503, I504, L506, Q509, S510, H511, T513, H515, G517, A519, S522, R528, K529, V531, V534, I535, T536, E537, I539, L540, F542, and R544. In some embodiments, the substitution is selected from the group consisting of E2H or I or S, A3E or G or K or N or Q or R or T, S13Q or T, D17E, Y18F or M or N, L19F, L20I or V, D23T, D25A or E or S, E26G or N or Q or T, S27E or F or K or V, 128E or F or M or P, E29D or P or R or T, V30N or Q, Y31Q or W, K32D or G or N or R, D33N, K34D or E or Q or S, K36F or R, K37F or I, A40C or D or E or F or M or N or P or Q or V, E41C or D or F or N or Q or S or V, V42A or S or T, R43I or Q, R44A or D or K or M or Y, E45C or M or N or Q, I46F or V, N47E or I or K or R or V, N48A or C or E or F or L or Q or R or S, E49G or H or I or R or S or W, K50C or G or M or N or P or R, A51E or G or L or Q or T, F53D, L54A or C or E or H or I or Q, T55A or H or N or Q or S or Y, L56H or Q or R or S, L57I, L59F or M or S or V or Y, I60C or V, N62V, R71I, E73D, S74G or M or P, D75E, R77A or N or T or V, G78E or I or K or N or P or Q or V or W, A79M or R or Y, D81C or E or H or L or N, R82C or F or G or L or W, F83G or H or I or L or V, V84F or H or L or N or Q or R or S or T or W or Y, S85C or L or N or R, S86C or N, G87C or E or F or K or L or N or T, G88C or D or I or V or W or Y, F89C or I, A91C or D or E or G or H or L or R or S or T or V or Y, V92A or C or E or F or G or I or L or Q or W, T93H or I or Q or V or W, K94C or V or Y, T95C or H or K or M, L97A or M or P, H98C or S or T or V or W, G99A or C or H or P or Q or T, T100A or I or L or M or V, A101S, L102M, S103A or C or G or L, L107C or F, Q109C or N or S, G111A, E113C or H or V, V114C, S115D or Y, Q116G or H or L or S or T or V, E117A or D or I, A118I or V, F119L or M, S120A or D or E or F or K or N or R or W or Y, G121D or L or V or W, K123I or S or W or Y, D124C or E, Q125A or D or G or H or K or L or N or S or T or V or W, G127D or F or W, N128A, F129L or Y, L130A or C or D or Q or V or Y, E131D or F or G or R, L133E or G or I or P or Q or T or V or Y, K134D or G or H or I or L or N or R or W or Y, E135H or S, D136N, I137A or C or D or G or P or Q or S or V, K138C or D or E or P or R or S or V, A139P or S or T or V, I140N or Q or S or T or V, L143A or F or G or N or R or W, A146M, L151E or G or M or N or Q or R or S or T or V or W, E152A or D or I or M or P, G153D, N155E or K or M, I156E or K or L or R or Y, D158E, A160F or H or S, K161L or R or S or Y, V162D or F or N or P or T, F163C or H or I or M or V or W or Y, S166C or E or H or K or P or Q or V or W, H167C or L or P, K169E or G or R, E170G or I or N or R, L171C or E or G or I or M or W, S172G or N or Q or R, K175A or G or H or N or P or T or V, I176A or C or N or Q or V, G177D or E or H or N or P or T, K178D or E or G or I or L or M or N or P or Q or V or Y, E179G or I or P or Q or S or T or V or W or Y, L180F or H or V or W, A181F or M or N or W, E182H or N, Q183A or L, N185D, A187C or S, H193W, R194I, T196V, Q197G, S204A or F or M or W or Y, K210M, K211D or E or F or G or H or I or M or R or S or T or V, E212A or D or M or P or Q or T, N215D or Y, Q216A or E or N, V217C or E or K or N or P or Q or T, L218V, L219I or M or V, E220D or N, A222S, I223C, L224A or C or T or V, Y226F, M228H or R, I229A, S231D or G or H or R or V, V232Q, R235A or D or N, T240V, S241C, R242K or L, R246H or Q, T251H, L253M, L260M, V268I, V270I, A271S, F272Q, Q275E, Y276F or H or Q, D278L or M or R or Y, S282C, E307Q or R, E314H, R315G or K, D317S, A320N or T, I321L or M, D323I or T, M328L, K329G or Q or R, C331T, F332Y, L333F, A343I or V, D345Y, N346A, K350H or W or Y, G351E or M, E352F or I or M or V, P356M or S, Y357E, K360Q, A361Q or S or V, A363S, D364N or T, C366A, N367D or E or M, A368D or Q, F369H or Q, L370A or D or E or F or H or N or R or S or T or V, Q371G or H or I or N or P or R or T or W or Y, N378D, K379E or R or S, S380K or N, T383Q, D386K or S, G389C or M or P or R or T, N390S, W392F or M, K393H or R, V402F or I or L, Y405F, V408Q or S, V409C or Q or S, Q410E or G or H or I or R, K413P, K414C or H or I or Q, E418N, K422G or H or Q or R, Y423G, H424D or G or I or S or T, D425P, T426A or M or Q, S428V, R429A or C or D or G or H or K or N, S431G, H432A or M, C437N, S444N or Q or T, I447K or R, A448H or S or T, S457D, M460A or E or G, R461N, T462S, K463G or N, G464A or D or E or F or H or V or Y, S466E or G or K or N or T, E467N, E468A or N or P or Q, L469A or N, T471N, E472A or G or N, M475I, K484A, K489R, L490I or Y, G492T or V, S493C or G or K or V, L494G or I or Q or V, K497M or T, V500I or Y, E501N, T502H, A503L or M, I504L, L506 I or V, Q509A, S510T, H511I or M, T513S, H515Q, G517P, A519C, S522A or K, R528K, K529A, V531G or N, V534A or S, I535C or S or T, T536M, E537H or N or Q, I539V, L540E or Q or R or V, F542M, and R544G or N or P or Q or S.
In another aspect, the invention provides for isolated polypeptides having improved isoprene synthase activity, wherein the polypeptide comprises one or more substitutions(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X22, X36, X43, X58, X87, X89, X118, X151, X234, X247, X254, X282, X288, X391, X392, X437, X443, X447, X481, X488, X502, and X542 and wherein the polypeptide has at least 30% increase in specific activity of isoprene synthase compared to wild-type isoprene synthase of SEQ ID NO:1. In some embodiments, the substitution is at a residue selected from the group consisting of S22, K36, R43, E58, G87, F89, A118, L151, Q234, V247, H254, 5282, S288, A391, W392, C437, I447, T481, E488, T502 and F542. In some embodiments, the substitution is selected from the group consisting of S22K or R, K36H or W, R43E, E58F, G87S or R, F89D, A118E, L151Y, G153P, Q234R, V247I, H254C, S282H or W, S288A or T or Y, A391G, W392C, C437L, I447V, T481Y, E488L, T502F and F542N.
In another aspect, the invention provides for isolated polypeptides having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide comprises one or more substitutions(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X30, X134, X143, X156, X159, X172, X414, and X421, and wherein a host cell expressing the polypeptide has at least 20% more growth compared to the growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1 under similar growth conditions. In some embodiments, the substitution is at a residue selected from the group consisting of K134, L143, I156, E159, S172, K414, and Q421. In some embodiments, the substitution is at a residue selected from the group consisting of V30K, K134C or P, L143I, I156G, E159D, S172V, K414F, and Q421R or D.
In another aspect, the invention provides for recombinant host cells comprising any of the polypeptides described above and in the rest of the specification. In some embodiments, the host cell is selected from the group consisting of a bacterial, algal, fungal, yeast, cyanobacterial, or Clostridial cell. In some embodiments, the host cell is a bacterial cell. In some embodiments, the bacterial cell is a gram-positive bacterial cell or gram-negative bacterial cell. In some embodiments, the bacterial cell is selected from the group consisting of E. coli, L. acidophilus, P. citrea, B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans, B. lautus, B. thuringiensis, S. albus, S. lividans, S. coelicolor, S. griseus, Pseudomonas sp., P. alcaligenes, Clostridium sp., Corynebacterium sp., and C. glutamicum cells. In some embodiments, the host cell is an algal cell. In some embodiments, the algal cell is selected from the group consisting of green algae, red algae, glaucophytes, chlorarachniophytes, euglenids, chromista, or dinoflagellates. In some embodiments, the host cell is a fungal cell. In some embodiments, the fungal cell is a filamentous fungi. In some embodiments, the host cell is a yeast cell. In some embodiments, the yeast cell is selected from the group consisting of Saccharomyces sp., Schizosaccharomyces sp., Pichia sp., or Candida sp. In some embodiments, the yeast cell is a Saccharomyces cerevisiae cell.
In another aspect, the invention provides for methods for identifying a polypeptide having improved isoprene synthase activity and/or improved growth characteristics when the polypeptide is expressed in a host cell, the method comprising screening a site evaluation library or combinatorial library for one or more substitution(s) in the polypeptide that improves specific activity and/or growth of the host cell when the polypeptide is expressed as compared to the specific activity and growth of a host cell expressing wild-type isoprene synthase of SEQ ID NO:1.
In another aspect, the invention provides for non-naturally occurring isoprene synthase variants comprising an amino acid residue substitution selected from the group consisting of: X003C, X003D, X003E, X003F, X003G, X003H, X003I, X003K, X003L, X003M, X003N, X003P, X003Q, X003R, X003S, X003T, X003V, X003W, X003Y, X007C, X007D, X007E, X007F, X007G, X007H, X007I, X007K, X007L, X007M, X007N, X007P, X007Q, X007R, X007S, X007T, X007V, X007W, X007Y, X009A, X009C, X009D, X009E, X009F, X009G, X009H, X009I, X009K, X009L, X009M, X009N, X009P, X009Q, X009R, X009S, X009T, X009V, X009W, X012A, X012C, X012D, X012E, X012F, X012G, X012H, X012I, X012K, X012L, X012M, X012P, X012Q, X012R, X012S, X012T, X012V, X012W, X012Y, X013A, X013C, X013D, X013E, X013F, X013G, X013H, X013I, X013K, X013L, X013M, X013N, X01P3, X013Q, X013R, X013T, X013V, X013W, X013Y, X016A, X016C, X016D, X016E, X016F, X016G, X016H, X016, X016K, X016L, X016M, X016N, X016P, X016Q, X016R, X016S, X016T, X016V, X016W, X018A, X018C, X018D, X0E18, X018F, X018G, X018H, X018I, X018K, X018L, X018M, X018N, X018P, X018Q, X018R, X018S, X018T, X018V, X018W, X020A, X020C, X020D, X020E, X020F, X020G, X020H, X020I, X020K, X020M, X020N, X020P, X020Q, X020R, X020S, X020T, X020V, X020W, X020Y, X023A, X023C, X023E, X023F, X023G, X023H, X023I, X023K, X023L, X023M, X023N, X023P, X023Q, X023R, X023S, X023T, X023V, X023W, X023Y, X025A, X025C, X025E, X025F, X025G, X025H, X025I, X025K, X025L, X025M, X025N, X025P, X025Q, X025R, X025S, X025T, X025V, X025W, X025Y, X026A, X026C, X026D, X026F, X026G, X026H, X026I, X026K, X026L, X026M, X026N, X026P, X026Q, X026R, X026S, X026T, X026V, X026W, X026Y, X027A, X027C, X027D, X027E, X027F, X027G, X027H, X027I, X027K, X027L, X027M, X027N, X027P, X027Q, X0R27, X027V, X027W, X027Y, X033A, X033C, X033E, X033F, X033G, X033H, X033I, X033K, X033L, X033M, X033N, X033P, X033Q, X033R, X033S, X033T, X033V, X033W, X033Y, X036A, X036C, X036D, X036E, X036F, X036G, X036G, X036I, X036L, X036M, X036N, X036P, X036Q, X036R, X036S, X036T, X036V, X036W, X036Y, X044A, X044C, X044D, X044E, X044F, X044G, X044H, X044I, X044K, X044L, X044M, X044N, X044P, X044Q, X044S, X044T, X044V, X044W, X044Y, X050A, X050C, X050D, X050E, X050F, X050G, X050H, X050I, X050L, X050M, X050N, X050P, X050Q, X050R, X050S, X050T, X050V, X050W, X050Y, X053A, X053C, X053D, X053E, X053G, X053H, X053I, X053K, X053L, X053M, X053N, X053P, X053Q, X053R, X053S, X053T, X053V, X053W, X053Y, X059A, X059C, X059D, X059E, X059F, X059G, X059H, X059I, X059K, X059M, X059N, X059P, X059Q, X059R, X059S, X059T, X059V, X059W, X059Y, X069A, X069C, X069D, X069E, X069F, X069H, X069I, X069K, X069L, X069M, X069N, X069P, X069Q, X069R, X069S, X069T, X069V, X069W, X069Y, X074A, X074C, X074D, X074E, X074F, X074G, X074H, X074I, X074K, X074L, X074M, X074N, X074P, X074Q, X074R, X074T, X074V, X074W, X074Y, X078A, X078C, X078D, X078E, X078F, X078H, X078I, X078K, X078L, X078M, X078N, X078P, X078Q, X078R, X078S, X078T, X078V, X078W, X078Y, X081A, X081C, X081E, X081F, X081G, X081H, X081I, X081K, X081L, X081M, X081N, X081P, X081Q, X081R, X081S, X081T, X081V, X081W, X081Y, X087A, X087C, X087D, X087E, X087F, X087H, X087I, X087K, X087L, X087M, X087N, X087P, X087Q, X087R, X087S, X087T, X087V, X087W, X087Y, X099A, X099C, X099D, X099E, X099F, X099H, X099I, X099K, X099L, X099M, X099N, X099P, X099Q, X099R, X099S, X099T, X099V, X099W, X099Y, X116A, X116C, X116D, X116E, X116F, X116G, X116H, X116I, X116K, X116L, X116M, X116N, X116P, X116R, X116S, X116T, X116V, X116W, X116Y, X117A, X117C, X117D, X117F, X117G, X117H, X117I, X117K, X117L, X117M, X117N, X117P, X117Q, X117R, X117S, X117T, X117V, X117W, X117Y, X120A, X120C, X120D, X120E, X120F, X120G, X120H, X120I, X120K, X120L, X120M, X120N, X120P, X120Q, X120R, X120T, X120V, X120W, X120Y, X121A, X121C, X121D, X121E, X121F, X121H, X121I, X121K, X121L, X121M, X121N, X121P, X121Q, X121R, X121S, X121T, X121V, X121W, X121Y, X125A, X125C, X125D, X125E, X125F, X125G, X125H, X125I, X125K, X125L, X125M, X125N, X125P, X125R, X125S, X125T, X125V, X125W, X125Y, X127A, X127C, X127D, X127E, X127F, X127H, X127I, X127K, X127L, X127M, X127N, X127P, X127Q, X127R, X127S, X127T, X127V, X127W, X127Y, X139C, X139D, X139E, X139F, X139G, X139H, X139I, X139K, X139L, X139M, X139N, X139P, X139Q, X139R, X139S, X139T, X139V, X139W, X139Y, X165A, X165C, X165D, X165E, X165F, X165G, X165H, X165K, X165L, X165M, X165N, X165P, X165Q, X165R, X165S, X165T, X165V, X165W, X165Y, X173A, X173C, X173D, X173F, X173G, X173H, X173I, X173K, X173L, X173M, X173N, X173P, X173Q, X173R, X173S, X173T, X173V, X173W, X173Y, X174A, X174C, X174D, X174F, X174G, X174H, X174I, X174K, X174L, X174M, X174N, X174P, X174Q, X174R, X174S, X174T, X174V, X174W, X174Y, X177A, X177C, X177D, X177E, X177F, X177H, X177I, X177K, X177L, X177M, X177N, X177P, X177Q, X177R, X177S, X177T, X177V, X177W, X177Y, X179A, X179C, X179D, X179F, X179G, X179H, X179I, X179K, X179L, X179M, X179N, X179P, X179Q, X179R, X179S, X179T, X179V, X179W, X179Y, X194A, X194C, X194D, X194E, X194F, X194G, X194H, X194I, X194K, X194L, X194M, X194N, X194P, X194Q, X194S, X194T, X194V, X194W, X194Y, X197A, X197C, X197D, X197E, X197F, X197G, X197H, X197I, X197K, X197L, X197M, X197N, X197P, X197R, X197S, X197T, X197V, X197W, X197Y, X202A, X202C, X202D, X202E, X202F, X202G, X202H, X202I, X202K, X202L, X202M, X202N, X202P, X202Q, X202R, X202S, X202T, X202W, X202Y, X216A, X216C, X216D, X216E, X216F, X216G, X216H, X216I, X216K, X216L, X216M, X216N, X216P, X216R, X216S, X216T, X216V, X216W, X216Y, X240A, X240C, X240D, X240E, X240F, X240G, X240H, X240I, X240K, X240L, X240M, X240N, X240P, X240Q, X240R, X240S, X240V, X240W, X240Y, X246A, X246C, X246D, X246E, X246F, X246G, X246H, X246I, X246K, X246L, X246M, X246N, X246P, X246Q, X246S, X246T, X246V, X246W, X246Y, X251A, X251C, X251D, X251E, X251F, X251G, X251H, X251I, X251K, X251L, X251M, X251N, X251P, X251Q, X251R, X251S, X251V, X251W, X251Y, X254A, X254C, X254D, X254E, X254F, X254G, X254I, X254K, X254L, X254M, X254N, X254P, X254Q, X254R, X254S, X254T, X254V, X254W, X254Y, X287A, X287C, X287D, X287E, X287G, X287H, X287I, X287K, X287L, X287M, X287N, X287P, X287Q, X287R, X287S, X287T, X287V, X287W, X287Y, X290A, X290C, X290D, X290E, X290F, X290G, X290H, X290I, X290K, X290L, X290M, X290N, X290P, X290Q, X290R, X290S, X290T, X290W, X290Y, X308A, X308C, X308D, X308E, X308F, X308G, X308H, X308I, X308K, X308M, X308N, X308P, X308Q, X308R, X308S, X308T, X308V, X308W, X308Y, X376A, X376C, X376D, X376E, X376F, X376G, X376H, X376I, X376K, X376M, X376N, X376P, X376Q, X376R, X376S, X376T, X376V, X376W, X376Y, X377A, X377C, X377D, X377E, X377F, X377G, X377H, X377I, X377K, X377L, X377M, X377N, X377P, X377Q, X377R, X377S, X377T, X377V, X377W, X379A, X379C, X379D, X379E, X379F, X379G, X379H, X379I, X379L, X379M, X379N, X379P, X379Q, X379R, X379S, X379T, X379V, X379W, X379Y, X389A, X389C, X389D, X389E, X389F, X389H, X389I, X389K, X389L, X389M, X389N, X389P, X389Q, X389R, X389S, X389T, X389V, X389W, X389Y, X397A, X397C, X397D, X397E, X397F, X397H, X397I, X397K, X397L, X397M, X397N, X397P, X397Q, X397R, X397S, X397T, X397V, X397W, X397Y, X400A, X400C, X400D, X400E, X400F, X400G, X400H, X400I, X400K, X400L, X400M, X400N, X400P, X400R, X400S, X400T, X400V, X400W, X400Y, X403A, X403C, X403D, X403E, X403G, X403H, X403I, X403K, X403L, X403M, X403N, X403P, X403Q, X403R, X403S, X403T, X403V, X403W, X403Y, X421A, X421C, X421D, X421E, X421F, X421G, X421H, X421I, X421K, X421L, X421M, X421N, X421P, X421R, X421S, X421T, X421V, X421W, X421Y, X426A, X426C, X426D, X426E, X426F, X426G, X426H, X426I, X426K, X426L, X426M, X426N, X426P, X426Q, X426R, X426S, X426V, X426W, X426Y, X430A, X430C, X430D, X430E, X430F, X430G, X430H, X430I, X430K, X430L, X430M, X430N, X430Q, X430R, X430S, X430T, X430V, X430W, X430Y, X434A, X434C, X434D, X434E, X434G, X434H, X434I, X434K, X434L, X434M, X434N, X434P, X434Q, X434R, X434S, X434T, X434V, X434W, X434Y, X445C, X445D, X445E, X445F, X445G, X445H, X445I, X445K, X445L, X445M, X445N, X445P, X445Q, X445R, X445S, X445T, X445V, X445W, X445Y, X448C, X448D, X448E, X448F, X448G, X448H, X448I, X448K, X448L, X448M, X448N, X448P, X448Q, X448R, X448S, X448T, X448V, X448W, X448Y, X457A, X457C, X457D, X457E, X457F, X457G, X457H, X457I, X457K, X457L, X457M, X457N, X457P, X457Q, X457R, X457T, X457V, X457W, X457Y, X462A, X462C, X462D, X462E, X462F, X462G, X462H, X462I, X462K, X462L, X462M, X462N, X462P, X462Q, X462R, X462S, X462V, X462W, X462Y, X476A, X476C, X476D, X476E, X476F, X476G, X476H, X476I, X476K, X476L, X476M, X476P, X476Q, X476R, X476S, X476T, X476V, X476W, X476Y, X487A, X487C, X487D, X487E, X487F, X487G, X487H, X487I, X487L, X487M, X487N, X487P, X487Q, X487R, X487S, X487T, X487V, X487W, X487Y, X488A, X488C, X488D, X488F, X488G, X488H, X488I, X488K, X488L, X488M, X488N, X488P, X488Q, X488R, X488S, X488T, X488V, X488W, X488Y, X489A, X489C, X489D, X489E, X489F, X489G, X489H, X489I, X489L, X489M, X489N, X489P, X489Q, X489R, X489S, X489T, X489V, X489W, X489Y, X490A, X490C, X490D, X490E, X490F, X490G, X490H, X490I, X490K, X490M, X490N, X490P, X490Q, X490R, X490S, X490T, X490V, X490W, X490Y, X491A, X491C, X491D, X491E, X491F, X491H, X491I, X491K, X491L, X491M, X491N, X491P, X491Q, X491R, X491S, X491T, X491V, X491W, X491Y, X492A, X492C, X492D, X492E, X492F, X492H, X492I, X492K, X492L, X492M, X492N, X492P, X492Q, X492R, X492S, X492T, X492V, X492W, X492Y, X493A, X493C, X493D, X493E, X493F, X493G, X493H, X493I, X493K, X493L, X493M, X493N, X493P, X493Q, X493R, X493T, X493V, X493W, X493Y, X495A, X495C, X495D, X495E, X495G, X495H, X495I, X495K, X495L, X495M, X495N, X495P, X495Q, X495R, X495S, X495T, X495V, X495W, X495Y, X496C, X496D, X496E, X496F, X496G, X496H, X496I, X496K, X496L, X496M, X496N, X496P, X496Q, X496R, X496S, X496T, X496V, X496W, X496Y, X497A, X497C, X497D, X497E, X497F, X497G, X497H, X497I, X497L, X497M, X497N, X497P, X497Q, X497R, X497S, X497T, X497V, X497W, X497Y, X498A, X498C, X498D, X498E, X498F, X498G, X498H, X498I, X498K, X498L, X498M, X498N, X498Q, X498R, X498S, X498T, X498V, X498W, X498Y, X509A, X509C, X509D, X509E, X509F, X509G, X509H, X509I, X509K, X509L, X509M, X509N, X509P, X509R, X509S, X509T, X509V, X509W, X509Y, X514A, X514C, X514D, X514E, X514F, X514G, X514H, X514I, X514K, X514L, X514M, X514N, X514P, X514Q, X514R, X514S, X514T, X514V, X514W, X521A, X521C, X521D, X521E, X521F, X521G, X521H, X521I, X521K, X521L, X521M, X521N, X521P, X521Q, X521R, X521S, X521V, X521W, X521Y, X539A, X539C, X539D, X539E, X539F, X539G, X539H, X539K, X539L, X539M, X539N, X539P, X539Q, X539R, X539S, X539T, X539V, X539W, X539Y, X540A, X540C, X540D, X540E, X540F, X540G, X540H, X540I, X540K, X540M, X540N, X540P, X540Q, X540R, X540S, X540T, X540V, X540W, X540Y, X544A, X544C, X544D, X544E, X544F, X544G, X544H, X544I, X544K, X544L, X544M, X544N, X544P, X544Q, X544S, X544T, X544V, X544W, and X544Y;
wherein X represents any amino acid; and
wherein each amino acid residue position is numbered by correspondence with an amino acid residue position in the P. alba isoprene synthase sequence as shown in FIG. 20.
In another embodiment, the variant comprises the amino acid residues: N438, E451, and Y514. In another embodiment, the variant comprises the amino acid residues: F287, G397, N438, E451, and Y514. In any of the embodiments, the variant comprises a substitution selected from the group consisting of: X003H, X003T, X033H, X033I, X033K, X033S, X033T, X033V, X033W, X033Y, X036L, X044F, X044H, X044T, X050I, X050L, X050W, X053I, X053L, X053T, X053V, X053W, X053Y, X059A, X059C, X059F, X059G, X059H, X059I, X059K, X059M, X059R, X069S, X074I, X074K, X074W, X078I, X078L, X078W, X078Y, X087K, X087L, X087T, X099I, X099K, X099L, X099T, X099V, X099Y, X116F, X116I, X116T, X116V, X116W, X116Y, X117F, X117I, X117L, X117W, X120I, X120L, X139T, X165H, X165Y, X173H, X173T, X173V, X173W, X174H, X174I, X177L, X177T, X177V, X179H, X179I, X179K, X179L, X179T, X179V, X179W, X202H, X254K, X376I, X377W, X389K, X421E, X421H, X421R, X448H, X448T, X448V, X462H, X462K, X462V, X476Y, X487T, X489I, X489R, X489T, X489W, X490H, X490I, X490T, X490V, X490W, X491H, X491I, X491K, X491L, X491T, X491V, X491W, X491Y, X492A, X492D, X492E, X492H, X492I, X492K, X492T, X492V, X493E, X493G, X493I, X493K, X493L, X493R, X493T, X493V, X493W, X495H, X495K, X495L, X495M, X495R, X495S, X495T, X495V, X495W, X495Y, X496H, X498H, X509I, X509T, X509V, X539L, X539T, X539V, X540H, X540I, X540K, X540T, X540V, X540Y, X544S, X544T, X544V, and X544W.
In any of the embodiments herein, the variant comprises a substitution selected from the group consisting of: X003T, X016I, X033F, X033H, X033I, X033V, X036I, X044F, X044M, X050H, X050I, X050T, X050W, X053I, X059C, X059E, X059F, X059H, X059I, X059K, X059Q, X059R, X059T, X069S, X069T, X074H, X074I, X074K, X074T, X074V, X074W, X078F, X078H, X078I, X078R, X078T, X078V, X078W, X078Y, X099I, X099K, X099T, X099V, X116F, X116I, X116N, X116P, X116T, X116V, X116W, X117C, X117F, X117I, X117L, X117M, X117W, X125F, X125V, X125W, X127H, X127T, X139T, X165F, X165H, X165K, X165Y, X173F, X173H, X173K, X173R, X173T, X173V, X173W, X174H, X174I, X174T, X179G, X179I, X179S, X216A, X421R, X448R, X448T, X448V, X462H, X462I, X462K, X462V, X462W, X476R, X476V, X487F, X487H, X487T, X487W, X489R, X490F, X490H, X490I, X490V, X490W, X491C, X491H, X491I, X491L, X491T, X491V, X491W, X492A, X492E, X493G, X493I, X493L, X493T, X493V, X493W, X495L, X498C, X540I, X540S, X540T, X540V, and X544K.
In any of the embodiments herein, the variant comprises a substitution selected from the group consisting of: X003T, X033H, X033I, X033V, X044F, X050I, X050W, X053I, X059C, X059F, X059H, X059I, X059K, X059R, X069S, X074I, X074K, X074W, X078I, X099I, X099K, X099T, X099V, X116F, X116I, X116T, X116V, X116W, X117F, X117I, X117L, X117W, X139T, X165H, X165Y, X173H, X173T, X173V, X173W, X174H, X174I, X078W, X078Y, X179I, X421R, X448T, X448V, X462H, X462K, X462V, X487T, X489R, X490H, X490I, X490V, X490W, X491I, X491H, X491L, X491T, X491V, X491W, X492A, X492E, X493G, X493I, X493L, X493T, X493V, X493W, X495L, X540I, X540T, X540V.
In any of the embodiments herein, the variant comprises a substitution selected from the group consisting of: X003F, X003H, X003I, X003K, X003R, X003T, X003Y, X013L, X016I, X016L, X016M, X018C, X018G, X020M, X020S, X020T, X020V, X020Y, X023H, X025H, X025I, X025K, X025L, X025T, X025V, X033F, X033H, X033I, X033K, X033L, X033Q, X033R, X033S, X033T, X033V, X033W, X033Y, X036I, X036L, X036R, X036T, X036V, X036W, X036Y, X044C, X044F, X044H, X044I, X044K, X044T, X044V, X044Y, X050H, X050I, X050L, X050T, X050V, X050W, X050Y, X053G, X053H, X053I, X053K, X053L, X053R, X053S, X053T, X053V, X053W, X053Y, X059A, X059C, X059D, X059E, X059F, X059G, X059H, X059I, X059K, X059M, X059N, X059Q, X059R, X059T, X059V, X059W, X069A, X069H, X069I, X069K, X069L, X069M, X069N, X069Q, X069R, X069S, X069T, X069V, X074H, X074I, X074K, X074L, X074T, X074V, X074W, X074Y, X078F, X078H, X078I, X078K, X078L, X078T, X078V, X078W, X078Y, X087H, X087I, X087K, X087L, X087M, X087R, X087T, X087V, X087W, X087Y, X099F, X099I, X099K, X099L, X099R, X099S, X099T, X099V, X099W, X099Y, X116A, X116F, X116I, X116K, X116L, X116S, X116T, X116V, X116W, X116Y, X117F, X117H, X117I, X117L, X117M, X117W, X120F, X120H, X120I, X120K, X120L, X120T, X120V, X120W, X120Y, X121F, X121H, X121I, X121K, X121L, X121T, X121V, X121W, X121Y, X125H, X125I, X125M, X125T, X125W, X125Y, X127F, X127H, X127I, X127L, X127T, X127V, X127Y, X139C, X139H, X139I, X139P, X139S, X139T, X139V, X165A, X165D, X165F, X165H, X165K, X165L, X165R, X165T, X165Y, X173F, X173G, X173H, X173I, X173K, X173L, X173M, X173R, X173S, X173T, X173V, X173W, X173Y, X174F, X174H, X174I, X174K, X174L, X174R, X174T, X174V, X174W, X174Y, X177A, X177H, X177I, X177K, X177L, X177M, X177P, X177T, X177V, X177Y, X179F, X179G, X179H, X179I, X179K, X179L, X179M, X179S, X179T, X179V, X179W, X179Y, X194H, X197H, X197I, X197M, X197T, X197V, X202F, X202H, X202I, X202K, X202R, X202T, X202Y, X246H, X246K, X246T, X251H, X251K, X251N, X251Y, X254F, X254I, X254K, X254R, X254T, X254V, X254W, X308H, X308I, X308W, X376I, X376Y, X377H, X377I, X377L, X377V, X377W, X379H, X379R, X379T, X379V, X389H, X389I, X389K, X389L, X389M, X389R, X389S, X389T, X389V, X389Y, X403T, X403V, X421E, X421G, X421H, X421I, X421K, X421L, X421R, X421T, X421V, X421W, X426I, X426V, X430S, X430T, X430V, X445H, X448H, X448I, X448R, X448S, X448T, X448V, X457H, X457Q, X457R, X457T, X462F, X462G, X462H, X462I, X462K, X462L, X462S, X462V, X462W, X462Y, X476R, X476T, X476V, X476W, X476Y, X487A, X487C, X487F, X487G, X487H, X487L, X487M, X487R, X487S, X487T, X487V, X487W, X489H, X489I, X489L, X489R, X489T, X489V, X489W, X490F, X490H, X490I, X490M, X490T, X490V, X490W, X491A, X491C, X491F, X491H, X491I, X491K, X491L, X491M, X491N, X491R, X491S, X491T, X491V, X491W, X491Y, X492A, X492C, X492D, X492E, X492H, X492I, X492K, X492L, X492R, X492T, X492V, X492W, X492Y, X493A, X493C, X493E, X493G, X493I, X493K, X493L, X493M, X493R, X493T, X493V, X493W, X493Y, X495A, X495G, X495H, X495I, X495K, X495L, X495M, X495Q, X495R, X495S, X495T, X495V, X495W, X495Y, X496H, X496I, X496K, X496L, X496R, X496T, X496V, X496Y, X497H, X497I, X497L, X497T, X497V, X498F, X498G, X498H, X498I, X498K, X498L, X498R, X498S, X498T, X498V, X498Y, X509I, X509M, X509S, X509T, X509V, X539K, X539L, X539T, X539V, X540E, X540F, X540G, X540H, X540I, X540K, X540M, X540Q, X540R, X540S, X540T, X540V, X540W, X540Y, X544C, X544H, X544I, X544K, X544L, X544S, X544T, X544V, and X544W.
In any of the embodiments herein, the variant comprises a substitution selected from the group consisting of: X003T, X013L, X117I, X165Y, X421R, X495L, X509T, and X540V. In any of the embodiments herein, the variant comprises X003T. In any of the embodiments herein, the variant comprises X495L. In any of the embodiments herein, the variant comprises X509T.
In any of the embodiments herein, a surface hydrophobic amino acid residue is substituted. In any of the embodiments herein, a symmetry contact amino acid residue is substituted. In any of the embodiments herein, a conservation amino acid residue is substituted. In any of the embodiments herein, an N-terminal loop amino acid residue is substituted. In any of the embodiments herein, a surface hydrophilic amino acid residue is substituted. In any of the embodiments herein, a surface loop amino acid residue is substituted. In any of the embodiments herein, an active site amino acid residue is substituted. In any of the embodiments herein, a flexible loop amino acid residue is substituted. In any of the embodiments herein, a hydrophobic pocket amino acid residue is substituted. In any of the embodiments herein, a C-terminal amino acid residue is substituted.
In any of the embodiments herein, amino acid residue 509 has a beta-branched carbon. In any of the embodiments herein, the variant has a N-terminal truncation.
In any of the embodiments herein, the N-terminal truncation comprises amino acid residues corresponding to those shown in FIG. 21A-21B.
In any of the embodiments herein, the variant is a variant of a plant isoprene synthase. In any of the embodiments herein, the variant is a poplar variant. In any of the embodiments herein, the variant is a P. alba variant. In any of the embodiments herein, the variant is a P. tremuloides variant. In any of the embodiments herein, the variant is a P. trichocharpa variant. In any of the embodiments herein, the variant is a P. nigra variant. In any of the embodiments herein, the variant is a P. alba v. tremuloides variant. In any of the embodiments herein, the variant is a kudzu variant. In any of the embodiments herein, the variant is an aspen variant. In any of the embodiments herein, the variant is an English oak variant. In any of the embodiments herein, the variant is a willow variant.
In any of the embodiments herein, the variant comprises an amino acid sequence having at least 40% sequence identity to a wild-type isoprene synthase. In any of the embodiments herein, the variant comprises an amino acid sequence having at least 60% sequence identity to a wild-type isoprene synthase. In any of the embodiments herein, the variant comprises an amino acid sequence having at least 80% sequence identity to a wild-type isoprene synthase. In any of the embodiments herein, the variant comprises an amino acid sequence having at least 90% sequence identity to a wild-type isoprene synthase.
In any of the embodiments herein, a host cell comprising a heterologous polynucleotide sequence encoding the variant in operable combination with a promoter has a growth index of at least about 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, or 1.4 as compared with MEA P. alba isoprene synthase.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein a host cell comprising a heterologous polynucleotide sequence encoding the variant in operable combination with a promoter has a performance index of at least about 0.8, 0.9, 1.0, or 1.1, as compared with MEA P. alba isoprene synthase.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein a host cell comprising a heterologous polynucleotide sequence encoding the variant in operable combination with a promoter has one or more of the following as compared with MEA P. alba isoprene synthase:
at least about 105% specific productivity,
at least about 105% yield, and
at least about 105% cellular performance index.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein a host cell comprising a heterologous polynucleotide sequence encoding the variant in operable combination with a promoter has one or more of the following as compared with MEA P. alba isoprene synthase:
at least about 110% specific productivity,
at least about 110% yield, and
at least about 110% cellular performance index.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein a host cell comprising a heterologous polynucleotide sequence encoding the variant in operable combination with a promoter has one or more of the following as compared with MEA P. alba isoprene synthase:
at least about 120% specific productivity,
at least about 120% yield, and
at least about 120% cellular performance index.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein a host cell comprising a heterologous polynucleotide sequence encoding the variant in operable combination with a promoter has one or more of the following as compared with MEA P. alba isoprene synthase:
at least about 150% specific productivity,
at least about 150% yield, and
at least about 150% cellular performance index.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein a host cell comprising a heterologous polynucleotide sequence encoding the variant in operable combination with a promoter has one or more of the following as compared with MEA P. alba isoprene synthase:
at least about 200% specific productivity,
at least about 200% yield, and
at least about 200% cellular performance index.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein the substitution comprises X491S.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein wherein the variant does not comprise a substitution selected from the group consisting of those listed in Table D.
In another aspect, the invention provides isoprene synthase variants of any of the embodiments herein, wherein the variant is not listed in Table E or Table F.
In another aspect, the invention provides compositions comprising any isoprene synthase variant described herein and a carrier.
In another aspect, the invention provides kits comprising any isoprene synthase variant described herein in a container.
In another aspect, the invention provides nucleic acids encoding any isoprene synthase variant described herein.
In another aspect, the invention provides compositions comprising nucleic acids encoding any isoprene synthase variant described herein and a carrier.
In another aspect, the invention provides kits comprising nucleic acids encoding any isoprene synthase variant described herein in a container.
In another aspect, the invention provides host cells comprising a heterologous polynucleotide sequence encoding any isoprene synthase variant described herein in operable combination with a promoter. In one embodiment, the polynucleotide sequence is contained within a plasmid. In another embodiment, the polynucleotide sequence is integrated into a chromosome of the host cell. In another embodiment, the host cell is selected from the group consisting of gram-positive bacterial cells, gram-negative bacterial cells, filamentous fungal cells, or yeast cells. In another embodiment, the host cell is a gram-negative bacterial cell. In another embodiment, the host cell is selected from the group consisting of Escherichia sp. (E. coli), Panteoa sp. (P. citrea), Bacillus sp. (B. subtilis), Yarrowia sp. (Y. lipolytica), and Trichoderma (T. reesei). In another embodiment, the host cell is E. coli. 
In any of the embodiments herein, the host cell is cultured in a medium comprising a carbon source selected from the group consisting of glucose, glycerol, glycerine, dihydroxyacetone, yeast extract, biomass, molasses, sucrose, and oil. In any of the embodiments herein, the host cell is cultured in a medium comprising glucose.
In any of the embodiments herein, the host cell further comprises a heterologous or native nucleic acid encoding an IDI polypeptide and/or a heterologous or native nucleic acid encoding a DXS polypeptide, optionally in combination with the native DXP pathway.
In any of the embodiments herein, the host cell comprises one vector encoding the isoprene synthase variant, the IDI polypeptide, and the DXS polypeptide.
In any of the embodiments herein, the host cell further comprises a heterologous nucleic acid encoding an MVA pathway polypeptide selected from the group consisting of an MVA pathway polypeptide from Saccharomyces cerevisiae and Enterococcus faecalis. 
In any of the embodiments herein, the host cell further comprises one or more nucleic acids encoding an MVA pathway polypeptide and a DXS polypeptide and wherein one vector encodes the isoprene synthase variant, the MVA pathway polypeptide, and the DXS polypeptide.
In any of the embodiments herein, the host cell further comprises one or more nucleic acids encoding a DXS polypeptide, an IDI polypeptide, one or more of the rest of the DXP pathway polypeptides, and/or a MVA pathway polypeptide.
In another aspect, the invention provides methods of producing a host cell capable of producing isoprene, the method comprising introducing a heterologous polynucleotide sequence encoding any isoprene synthase variant described herein into the host cell.
In another aspect, the invention provides methods of producing isoprene, comprising: (a) culturing the host cells comprising heterologous polynucleotide sequences encoding any isoprene synthase variant described herein under suitable culture conditions for production of isoprene; and (b) producing the isoprene. In one embodiment, the method further comprises (c) recovering the isoprene.
In another aspect, the invention provides methods of producing isoprene, comprising: (a) providing: (i) a host cell; and (ii) a nucleic acid encoding any isoprene synthase variant described herein in operable combination with a promoter; (b) introducing the nucleic acid into the host cell to produce a transformed host cell; and (c) culturing the transformed host cells under suitable culture conditions for production of isoprene. In one embodiment, the method further comprises (d) recovering the isoprene.
In another aspect, the invention provides methods of producing an isoprene synthase variant, comprising: (a) providing: (i) a host cell; and (ii) a nucleic acid encoding any isoprene synthase variant described herein in operable combination with a promoter; (b) introducing the nucleic acid into the host cell to produce a transformed host cell; and (c) culturing the transformed host cells under suitable culture conditions for production of the isoprene synthase variant. In one embodiment, the method further comprises isolating the isoprene synthase variant.
In another aspect, the invention provides methods of screening an isoprene synthase variant, comprising: (a) contacting a host cell with a medium comprising about 10 μM to about 70 μM IPTG, and about 5 mM to about 20 mM mevalonic acid (MVA), wherein the host cell comprises a nucleic acid encoding an isoprene synthase variant in operable combination with a promoter; and (b) measuring the growth rate of the host cell; wherein an increased growth rate indicates an isoprene synthase variant with an increased ability to convert DMAPP to isoprene within the host cell. In one embodiment, the IPTG is present in the medium at a concentration from about 10 μM to about 60 μM. In another embodiment, the IPTG is present in the medium at a concentration from about 20 μM to about 60 μM. In another embodiment, the is present in the medium at a concentration from about 40 μM to about 60 μM. In another embodiment, the IPTG is present in the medium at a concentration of about 50 μM. In another embodiment, the MVA is present in the medium at a concentration of about 5 mM to about 20 mM. In another embodiment, the MVA is present in the medium at a concentration of about 7 mM to about 15 mM. In another embodiment, the MVA is present in the medium at a concentration of about 8 mM to about 12 mM. In another embodiment, the MVA is present in the medium at a concentration of about 10 mM. In one embodiment, the host cell is MD09-170.
Also provided is an expression vector comprising a polynucleotide sequence encoding the isoprene synthase variant in operable combination with a promoter.
Also provided is a lysate of the host cell, wherein the lysate further comprises lysozyme. In some embodiments, the lysate has a neutral pH (6.5 to 7.5), while in other embodiments the lysate has a basic pH (above 7.5 and below 9.5). The present invention further provides methods of detecting isoprene synthase activity, comprising: (a) culturing host cells comprising the expression vector under conditions suitable for producing the isoprene synthase variant; (b) lysing the host cells with a lysis buffer comprising lysozyme to produce a cell lysate; and (c) detecting isoprene synthase activity in the cell lysate by measuring isoprene production from dimethylallyl diphosphate (DMAPP).



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the relationship between growth and DMAPP concentration in assay strain. DW425 was grown in the presence of various concentrations of mevalonate (0, 10, 20, 30, 40, 50 mM mevalonate in exp1, and 0, 2.5, 5, 10, 20 mM mevalonate in expt2) and IPTG (0 μM IPTG and 50 μM IPTG). Cells were harvested and collected for metabolite analysis upon completion of the growth experiment.
FIG. 2 shows DW425 growth in the presence of varying concentrations of IPTG (0, 10, 20, 30, 40, 50, 60, and 70 μM) and mevalonate (0, 5, 7.5, 10, 15, 20 mM).
FIG. 3 shows growth as a function of [IPTG] and [mevalonate].
FIG. 4 shows a map of pCL201.
FIG. 5 shows a view of Wild Type IspS showing the location of Phe495 with respect to Gly491 and Leu494, all in stick representation.
FIG. 6 shows a view of Wild Type IspS showing the location of Gln509, in stick representation, with respect to the active site. The magnesium ions and (+)-bornyl diphosphate in the active site are modeled based on a structural alignment with PDB 1N24.
FIG. 7 shows a view of Wild Type IspS showing residue Phe287 in stick representation. (+)-bornyl diphospate (BP) is placed based on a structural alignment with PDB 1N24.
FIG. 8 shows a view of Wild Type IspS showing residue Gly397 in stick representation.
FIG. 9 shows an active site view of Wild Type IspS showing residue Asn438 in stick representation. (+)-bornyl diphospate (BP) and Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 10 shows a view of Wild Type IspS showing residue Glu451 in stick representation.
FIG. 11 shows an active site view of Wild Type IspS showing residue Tyr514 in stick representation. (+)-bornyl diphospate (BP) and Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 12 shows a map of pCHL243, containing P. alba Isoprene Synthase with the G491S mutation.
FIG. 13 shows the ratio between isoprene concentration (%) and CO2(%) in the offgas, showing that strain DW415 expressing the G491S mutant of isoprene synthase has increased levels of isoprene production when compared to respiration rate throughout significant parts of the fermentation.
FIG. 14 shows expression of the isoprene synthase variant G491S results in increased viability during fermentation. Staining with DiBAC4(3) was used to determine the fraction of live and dead cells in the fermentation broth based on the presence or absence of a membrane potential.
FIG. 15 is a map of plasmid MD09-163 with G491S.
FIG. 16 shows a SDS-PAGE gel showing IspS-G491S at different stages of the purification. Lane 1—molecular weight standard; Lane 2-IspS-G491S digested with TurboTEV; Lanes 3-5-IspS-G491S in flow-though after second Ni column; Lanes 6-7-IspS-G491S fractions from second Ni column.
FIG. 17 shows IspS-G491S forming a dimer. Monomer A is light grey, monomer B is dark grey.
FIG. 18 is an 2FO-2FC electron density map at residue 491 contoured at 1-sigma. A shows 491G, B shows 491S.
FIG. 19 is an alignment of the loop containing residue 491. Wild type IspS is light grey and IspS-G491S is dark grey. The G491S mutation results in an alternate loop structure.
FIG. 20A-B depicts the sequence MEA P. alba (SEQ ID NO: 1).
FIGS. 21A-B show the alignment of N-terminally cleaved P. alba IspS sequences (SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:1, SEQ ID NO:40).
FIG. 22 shows the MVA and DXP metabolic pathways for isoprene (based on F. Bouvier et al., Progress in Lipid Res. 44: 357-429, 2005). The following description includes alternative names for each polypeptide in the pathways and a reference that discloses an assay for measuring the activity of the indicated polypeptide (each of these references are each hereby incorporated by reference in their entireties, particularly with respect to assays for polypeptide activity for polypeptides in the MVA and DXP pathways). Mevalonate Pathway: AACT; Acetyl-CoA acetyltransferase, MvaE, EC 2.3.1.9. Assay: J. Bacteriol., 184: 2116-2122, 2002; HMGS; Hydroxymethylglutaryl-CoA synthase, MvaS, EC 2.3.3.10. Assay: J. Bacteriol., 184: 4065-4070, 2002; HMGR; 3-Hydroxy-3-methylglutaryl-CoA reductase, MvaE, EC 1.1.1.34. Assay: J. Bacteriol., 184: 2116-2122, 2002; MVK; Mevalonate kinase, ERG12, EC 2.7.1.36. Assay: Curr Genet. 19:9-14, 1991. PMK; Phosphomevalonate kinase, ERGS, EC 2.7.4.2, Assay: Mol Cell Biol., 11:620-631, I991; DPMDC; Diphosphomevalonate decarboxylase, MVD1, EC 4.1.1.33. Assay: Biochemistry, 33:13355-13362, 1994; IDI; Isopentenyl-diphosphate delta-isomerase, IDI1, EC 5.3.3.2. Assay: J. Biol. Chem. 264:19169-19175, I989. DXP Pathway: DXS; 1-Deoxyxylulose-5-phosphate synthase, dxs, EC 2.2.1.7. Assay: PNAS, 94:12857-62, 1997; DXR; 1-Deoxy-D-xylulose 5-phosphate reductoisomerase, dxr, EC 2.2.1.7. Assay: Eur. J. Biochem. 269:4446-4457, 2002; MCT; 4-Diphosphocytidyl-2C-methyl-D-erythritol synthase, IspD, EC 2.7.7.60. Assay: PNAS, 97: 6451-6456, 2000; CMK; 4-Diphosphocytidyl-2-C-methyl-D-erythritol kinase, IspE, EC 2.7.1.148. Assay: PNAS, 97:1062-1067, 2000; MCS; 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase, IspF, EC 4.6.1.12. Assay: PNAS, 96:11758-11763, I999; HDS; 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, ispG, EC 1.17.4.3. Assay: J. Org. Chem., 70:9168-9174, 2005; HDR; 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, IspH, EC 1.17.1.2. Assay: JACS, 126:12847-12855, 2004.
FIG. 23 shows a monomer view of wild type IspS showing the location of sites where substitutions are not tolerated.
FIG. 24 shows the location of residues in the active site of IspS that do not tolerate substitution. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 25 shows the location of buried sites in IspS that do not tolerate substitution. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 26 shows residues located at the dimer interface of IspS that do not tolerate substitution. Chain A is light gray and chain B is dark gray.
FIG. 27 shows the location of N-terminally located or interacting sites of IspS that do not tolerate substitution. Mg2+ (spheres) and residues 1-50 are modeled based on a structural alignment with PDB 1N24.
FIG. 28 shows the location of N-terminally located or interacting sites of IspS that do not tolerate substitution. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 29 shows the proposed substrate capture loop positions of IspS that do not tolerate substitution. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 30 shows the monomer view of wild type IspS showing the location of sites where variants demonstrate improved specific activity.
FIG. 31 shows the location of buried sites that are in or interact with the N-terminus of IspS, where variants demonstrated increased specific activity.
FIG. 32 shows the location of buried sites that are in or interact with the C-terminus of IspS, where variants demonstrated increased specific activity. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24
FIG. 33 shows the dimer interface of IspS, with chain A in light gray and chain B in dark gray. Variants at position 247 demonstrated improved specific activity.
FIG. 34 shows the location of N-terminal sites of IspS where variants demonstrated improved specific activity.
FIG. 35 shows the surface loop positions of IspS where variants demonstrated improved specific activity. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 36 shows the surface positions of IspS where variants demonstrated improved specific activity. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 37 shows the proposed substrate capture loop positions of IspS where variants demonstrated improved specific activity. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 38 shows positions of IspS demonstrating improved activity. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 39 shows positions of IspS demonstrating improved activity. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 40 shows monomer view of IspS showing N-terminal and Surface Loop positions demonstrating improved growth. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 41 shows monomer view of IspS showing N-terminal Helix positions demonstrating improved growth.
FIG. 42 shows monomer view of wild type IspS showing the location of sites from Table 23 (A). Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24. B) Close-up view of sites in A.
FIG. 43 shows monomer view of IspS with location of sites where variants demonstrate improved growth colored dark gray. Mg2+ (spheres) are placed based on a structural alignment with PDB 1N24.
FIG. 44 shows view of IspS with location of sites where variants demonstrate improved growth shown as sticks. A and B are separated by 180°.
FIG. 45 shows an alignment of N-terminally truncated P. alba IspS molecules (SEQ ID NO:41, SEQ ID NO:1, SEQ ID NO:34, SEQ ID NO:36).
FIG. 46 shows a plasmid map of pDW207 harboring the P. alba IspS MAR variant.
FIG. 47 shows a plasmid map of pDW208 harboring the P. alba IspS MRR variant.



GENERAL DESCRIPTION OF THE INVENTION
The present invention provides methods and compositions comprising at least one isoprene synthase variant enzyme. The variant comprises one or more amino acid residue substitution(s) from a parent isoprene synthase polypeptide, wherein the parent isoprene synthase may be a wild type or non-wild type sequence. The invention provides amino acid residue substitutions at particular positions within the polypeptide, wherein the substitution may result in at least one improved property as compared to its parent sequence or a reference sequence. In some particularly preferred embodiments, at least one improved property is selected from but not limited to the group consisting of: specific productivity, yield, and cellular performance index. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber, polymers, and elastomers.
The practice of the present invention will employ, unless otherwise indicated, conventional techniques of protein chemistry, molecular biology (including recombinant techniques), microbiology, cell biology, biochemistry, nucleic acid chemistry, and enzymology, which are within the skill of the art. Such techniques are explained fully in the literature, such as, Molecular Cloning: A Laboratory Manual, second edition (Sambrook et al., 1989) and Molecular Cloning: A Laboratory Manual, third edition (Sambrook and Russel, 2001), (jointly and individually referred to herein as “Sambrook”). Oligonucleotide Synthesis (M. J. Gait, ed., 1984); Animal Cell Culture (R. I. Freshney, ed., 1987); Handbook of Experimental Immunology (D. M. Weir & C. C. Blackwell, eds.); Gene Transfer Vectors for Mammalian Cells (J. M. Miller & M. P. Calos, eds., 1987); Current Protocols in Molecular Biology (F. M. Ausubel et al., eds., 1987, including supplements through 2001); PCR: The Polymerase Chain Reaction, (Mullis et al., eds., 1994); Current Protocols in Nucleic Acid Chemistry John Wiley & Sons, Inc., New York, 2000); and Agrawal, ed., Protocols for Oligonucleotides and Analogs, Synthesis and Properties Humana Press Inc., New Jersey, 1993). Accordingly, the terms defined immediately below are more fully described by reference to the Specification as a whole.
Furthermore, the headings provided herein are not limitations of the various aspects or embodiments of the invention, which can be had by reference to the specification as a whole. Accordingly, the terms defined immediately below are more fully defined by reference to the specification as a whole. Nonetheless, in order to facilitate understanding of the invention, a number of terms are defined below.
DEFINITIONS
Unless defined otherwise herein, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although any methods and materials similar or equivalent to those described herein find use in the practice of the present invention, the preferred methods and materials are described herein. Accordingly, the terms defined immediately below are more fully described by reference to the Specification as a whole.
“X” refers to any amino acid residue. However, when in the context of an amino acid substitution (e.g. “X003C”), it is to be understood that “X” refers to an amino acid residue other than the amino acid residue resulting from the substitution (e.g., X is an amino acid residue other than C). In some embodiments, the additional zeros in front of the residue position are not included, thus for example “X003” can also be referred to as “X3” to refer to residue position 3.
“Isoprene” refers to 2-methyl-1,3-butadiene (CAS#78-79-5). It can refer to the direct and final volatile C5 hydrocarbon product from the elimination of pyrophosphate from 3,3-dimethylallyl pyrophosphate (DMAPP). It may not involve the linking or polymerization of one or more isopentenyl diphosphate (IPP) molecules to one or more DMAPP molecules. Isoprene is not limited by the method of its manufacture.
As used herein, the terms “isoprene synthase,” “isoprene synthase variant”, and “IspS,” refer to enzymes that catalyze the elimination of pyrophosphate from diemethylallyl diphosphate (DMAPP) to form isoprene. An “isoprene synthase” may be a wild type sequence or an isoprene synthase variant.
An “isoprene synthase variant” indicates a non-wild type polypeptide having isoprene synthase activity. One skilled in the art can measure isoprene synthase activity using known methods. See, for example, by GC-MS (see, e.g., WO 2009/132220, Example 3) or Silver et al., J. Biol. Chem. 270:13010-13016, I995. Variants may have substitutions, additions, deletions, and/or truncations from a wild type isoprene synthase sequence. Variants may have substitutions, additions, deletions, and/or truncations from a non-wild type isoprene synthase sequence. The variants described herein contain at least one amino acid residue substitution from a parent isoprene synthase polypeptide. In some embodiments, the parent isoprene synthase polypeptide is a wild type sequence. In some embodiments, the parent isoprene synthase polypeptide is a non-wild type sequence. In various embodiments, the variant will have at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 100%, at least about 110%, at least about 120%, at least about 130%, at least about 140%, at least about 150%, at least about 160%, at least about 170%, at least about 180%, at least about 190%, at least about 200% of the activity of a wild type isoprene synthase. In various embodiments, the variant will have at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99% sequence identity to a wild type isoprene synthase. In various embodiments, the number of differing amino acid residues between the variant and the wild type may be one or more, e.g. 1, 2, 3, 4, 5, 10, 15, 20, 30, 40, 50, or more amino acid residues. Wild type isoprene synthases can include any isoprene synthases from plants, for example, kudzu isoprene synthases, poplar isoprene synthases, English oak isoprene synthases, and willow isoprene synthases.
As used herein, the term “naturally-occurring” refers to anything (e.g., proteins, amino acids, or nucleic acid sequences) that is found in nature (e.g., has not been manipulated by means of recombinant or chemical methods). As used herein, the term “non-naturally occurring” refers to anything that is not found in nature (e.g., recombinantly produced or chemically synthesized proteins, amino acids, or nucleic acid sequences produced in the laboratory).
As used herein, an amino acid residue of an amino acid sequence of interest that “corresponds to” or is “corresponding to” or in “correspondence with” an amino acid residue of a reference amino acid sequence indicates that the amino acid residue of the sequence of interest is at a location homologous or equivalent to an enumerated residue in the reference amino acid sequence. One skilled in the art can determine whether a particular amino acid residue position in a polypeptide corresponds to that of a homologous reference sequence. For example, the sequence of an isoprene synthase polypeptide may be aligned with that of a reference sequence (e.g. SEQ ID NO: 1 (FIG. 20) using known techniques (e.g., basic local alignment search tool (BLAST), ClustalW2, Structure based sequences alignment program (STRAP), or the like). In addition, crystal structure coordinates of a reference sequence may be used as an aid in determining a homologous polypeptide residue's three dimensional structure (see, for example, PCT/US2010/032134 (WO 2010/124146)). In another aspect, equivalent residues may be identified by determining homology at the level of tertiary structure. Using such methods, the amino acid residues of an isoprene synthase polypeptide or isoprene synthase variant may be numbered according to the corresponding amino acid residue position numbering of the reference sequence. For example, the amino acid sequence of SEQ ID NO: 1 (FIG. 20) may be used for determining amino acid residue position numbering of each amino acid residue of an isoprene synthase variant of interest.
The term “identical” in the context of two nucleic acids or polypeptide sequences refers to the residues in the two sequences that are the same when aligned for maximum correspondence, as measured using one of the following sequence comparison or analysis algorithms.
As used herein, “homology” refers to sequence similarity or identity, with identity being preferred. Homology may be determined using standard techniques known in the art (see, e.g., Smith and Waterman, Adv. Appl. Math. 2:482 [1981]; Needleman and Wunsch, J. Mol. Biol. 48:443 [1970\; Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]; software programs such as GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package (Genetics Computer Group, Madison, Wis.); and Devereux et al., Nucl. Acid Res. 12:387-395 [1984]). One example of a useful algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pair-wise alignments. It can also plot a tree showing the clustering relationships used to create the alignment. PILEUP uses a simplification of the progressive alignment method of Feng and Doolittle (see Feng and Doolittle, J. Mol. Evol. 35:351-360 [1987]). The method is similar to that described by Higgins and Sharp (see Higgins and Sharp, CABIOS 5:151-153 [1989]). Useful PILEUP parameters including a default gap weight of 3.00, a default gap length weight of 0.10, and weighted end gaps. Another example of a useful algorithm is the BLAST algorithm, described by Altschul et al., (see Altschul et al., J. Mol. Biol. 215:403-410 [1990]; and Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787 [1993]). A particularly useful BLAST program is the WU-BLAST-2 program (see Altschul et al., Meth. Enzymol. 266:460-480 [1996]). WU-BLAST-2 uses several search parameters, most of which are set to the default values. The adjustable parameters are set with the following values: overlap span=1, overlap fraction=0.125, word threshold (T)=11. The HSP S and HSP S2 parameters are dynamic values and are established by the program itself depending upon the composition of the particular sequence and composition of the particular database against which the sequence of interest is being searched. However, the values may be adjusted to increase sensitivity.
The percent sequence identity between a reference sequence and a test sequence of interest may be readily determined by one skilled in the art. The percent identity shared by polynucleotide or polypeptide sequences is determined by direct comparison of the sequence information between the molecules by aligning the sequences and determining the identity by methods known in the art. An example of an algorithm that is suitable for determining sequence similarity is the BLAST algorithm, (see Altschul, et al., J. Mol. Biol., 215:403-410 [1990]). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence that either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. These initial neighborhood word hits act as starting points to find longer HSPs containing them. The word hits are expanded in both directions along each of the two sequences being compared for as far as the cumulative alignment score can be increased. Extension of the word hits is stopped when: the cumulative alignment score falls off by the quantity X from a maximum achieved value; the cumulative score goes to zero or below; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLAST program uses as defaults a wordlength (W) of 11, the BLOSUM62 scoring matrix (see Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1992]) alignments (B) of 50, expectation (E) of 10, M′S, N′-4, and a comparison of both strands.
The BLAST algorithm then performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul, supra). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a isoprene synthase nucleic acid of this invention if the smallest sum probability in a comparison of the test nucleic acid to a isoprene synthase nucleic acid is less than about 0.1, more preferably less than about 0.01, and most preferably less than about 0.001. Where the test nucleic acid encodes an isoprene synthase polypeptide, it is considered similar to a specified isoprene synthase nucleic acid if the comparison results in a smallest sum probability of less than about 0.5, and more preferably less than about 0.2.
Percent “identical” or “identity” in the context of two or more nucleic acid or polypeptide sequences refers to two or more sequences that are the same or have a specified percentage of nucleic acid residues or amino acid residues, respectively, that are the same, when compared and aligned for maximum similarity, as determined using a sequence comparison algorithm or by visual inspection. “Percent sequence identity” or “% identity” or “% sequence identity or “% amino acid sequence identity” of a subject amino acid sequence to a reference amino acid sequence means that the subject amino acid sequence is identical (i.e., on an amino acid-by-amino acid basis) by a specified percentage to the reference amino acid sequence over a comparison length when the sequences are optimally aligned. Thus, 80% amino acid sequence identity or 80% identity with respect to two amino acid sequences means that 80% of the amino acid residues in two optimally aligned amino acid sequences are identical.
“Percent sequence identity” or “% identity” or “% sequence identity” of a subject nucleic acid sequence to a reference nucleic acid sequence means that the subject nucleic acid sequence is identical (i.e., on a nucleotide-by-nucleotide basis for a polynucleotide sequence) by a specified percentage to the reference sequence over a comparison length when the sequences are optimally aligned. Thus, 80% nucleotide sequence identity or 80% identity with respect to two nucleic acid sequences means that 80% of the nucleotide residues in two optimally aligned nucleic acid sequences are identical.
The “percent sequence identity” or “% sequence identity” or “% identity” of a subject sequence to a reference sequence can be calculated by optimally aligning the two sequences and comparing the two optimally aligned sequences over the comparison length. The number of positions in the optimal alignment at which identical residues occur in both sequences is determined, thereby providing the number of matched positions, and the number of matched positions is then divided by the total number of positions of the comparison length (which, unless otherwise specified, is the length of the reference sequence). The resulting number is multiplied by 100 to yield the percent sequence identity of the subject sequence to the reference sequence.
“Optimal alignment” or “optimally aligned” refers to the alignment of two (or more) sequences giving the highest percent identity score. For example, optimal alignment of two polypeptide sequences can be achieved by manually aligning the sequences such that the maximum number of identical amino acid residues in each sequence are aligned together or by using software programs or procedures described herein or known in the art. Optimal alignment of two nucleic acid sequences can be achieved by manually aligning the sequences such that the maximum number of identical nucleotide residues in each sequence are aligned together or by using software programs or procedures described herein or known in the art.
Two sequences (e.g., polypeptide sequences) may be deemed “optimally aligned” when they are aligned using defined parameters, such as a defined amino acid substitution matrix, gap existence penalty (also termed gap open penalty), and gap extension penalty, so as to achieve the highest similarity score possible for that pair of sequences. The BLOSUM62 scoring matrix (see Henikoff and Henikoff, supra) is often used as a default scoring substitution matrix in polypeptide sequence alignment algorithms (e.g., BLASTP). The gap existence penalty is imposed for the introduction of a single amino acid gap in one of the aligned sequences, and the gap extension penalty is imposed for each residue position in the gap. Exemplary alignment parameters employed are: BLOSUM62 scoring matrix, gap existence penalty=11, and gap extension penalty=1. The alignment score is defined by the amino acid positions of each sequence at which the alignment begins and ends (e.g., the alignment window), and optionally by the insertion of a gap or multiple gaps into one or both sequences, so as to achieve the highest possible similarity score.
Optimal alignment between two or more sequences can be determined manually by visual inspection or by using a computer, such as, but not limited to e.g., the BLASTP program for amino acid sequences and the BLASTN program for nucleic acid sequences (see, e.g., Altschul et al., Nucleic Acids Res. 25(17):3389-3402 (1997); see also the National Center for Biotechnology Information (NCBI) website) or CLUSTALW program.
A polypeptide of interest may be said to be “substantially identical” to a reference polypeptide if the polypeptide of interest comprises an amino acid sequence having at least about 60%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the amino acid sequence of the reference polypeptide. The percent identity between two such polypeptides can be determined manually by inspection of the two optimally aligned polypeptide sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two polypeptides are substantially identical is that the first polypeptide is immunologically cross-reactive with the second polypeptide. Typically, polypeptides that differ by conservative amino acid substitutions are immunologically cross-reactive. Thus, a polypeptide is substantially identical to a second polypeptide, e.g., where the two peptides differ only by a conservative amino acid substitution or one or more conservative amino acid substitutions.
A nucleic acid of interest may be said to be “substantially identical” to a reference nucleic acid if the nucleic acid of interest comprises a nucleotide sequence having at least about 60%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% sequence identity to the nucleotide sequence of the reference nucleic acid. The percent identity between two such nucleic acids can be determined manually by inspection of the two optimally aligned nucleic acid sequences or by using software programs or algorithms (e.g., BLAST, ALIGN, CLUSTAL) using standard parameters. One indication that two nucleic acid sequences are substantially identical is that the two nucleic acid molecules hybridize to each other under stringent conditions (e.g., within a range of medium to high stringency).
A “nucleic acid” refers to two or more deoxyribonucleotides and/or ribonucleotides in either single or double-stranded form. It is to be understood that mutations, including single nucleotide mutations, can occur within a nucleic acid as defined herein.
A “recombinant nucleic acid” refers to a nucleic acid of interest that is free of one or more nucleic acids (e.g., genes) which, in the genome occurring in nature of the organism from which the nucleic acid of interest is derived, flank the nucleic acid of interest. The term therefore includes, for example, a recombinant DNA which is incorporated into a vector, into an autonomously replicating plasmid or virus, or into the genomic DNA of an anaerobic microorganism, or which exists as a separate molecule (e.g., a cDNA, a genomic DNA fragment, or a cDNA fragment produced by PCR or restriction endonuclease digestion) independent of other sequences. A recombinant nucleic acid may be obtained using molecular biology techniques that are known in the art, or part or all of a recombinant nucleic acid may be chemically synthesized.
A “heterologous nucleic acid” can be a nucleic acid whose nucleic acid sequence is from another species than the host cell or another strain of the same species of the host cell. In some embodiments, the sequence is not identical to that of another nucleic acid naturally found in the same host cell. In some embodiments, a heterologous nucleic acid is not identical to a wild-type nucleic acid that is found in the same host cell in nature.
An “endogenous nucleic acid” is a nucleic acid whose nucleic acid sequence is naturally found in the host cell. In some embodiments, an endogenous nucleic acid is identical to a wild-type nucleic acid that is found in the host cell in nature. In some embodiments, one or more copies of endogenous nucleic acids are introduced into a host cell.
A nucleic acid or protein of the invention may be in isolated or purified form. As used herein, “isolated,” with respect to nucleic acid or protein, means separated from other components, such as, but not limited to a cell or cell culture. It is preferably in a homogeneous state although it can be in either a dry or aqueous solution. Purity and homogeneity are typically determined using analytical chemistry techniques, such as polyacrylamide gel electrophoresis or high performance liquid chromatography. A protein or nucleic acid that is the predominant species present in a preparation is substantially purified. The term “purified” denotes that a nucleic acid or protein gives rise to essentially one band in an electrophoretic gel. Particularly, “purified” means that when isolated, the isolate contains at least 90%, at least 95%, at least 98%, or more preferably at least 99% of nucleic acid or protein by weight of the isolate.
Purified polypeptides may be obtained by a number of methods including, for example, laboratory synthesis, chromatography, preparative electrophoresis, gel electrophoresis, centrifugation, precipitation, affinity purification, etc. (see, generally, R Scopes, Protein Purification, Springer-Verlag, N.Y. (1982), Deutscher, Methods in Enzymology Vol. 182: Guide to Protein Purification, Academic Press, Inc. N.Y. (1990)).
“Polypeptides” includes polypeptides, proteins, peptides, fragments of polypeptides, and fusion polypeptides. It is also understood that a polypeptide may be coded for by more than one nucleotide sequence due to the degeneracy of the genetic code.
A “heterologous polypeptide” is a polypeptide encoded by a heterologous nucleic acid. In some embodiments, the sequence is not identical to that of another polypeptide encoded by a nucleic acid naturally found in the same host cell. Examples of heterologous proteins include enzymes such as isoprene synthases. In some embodiments, the genes encoding the proteins are naturally occurring genes, while in other embodiments mutated and/or synthetic genes are used.
An “endogenous polypeptide” is a polypeptide whose amino acid sequence is naturally found in the host cell. In some embodiments, an endogenous polypeptide is identical to a wild-type polypeptide that is found in the host cell in nature.
As used herein, the term “terpenoid” or “isoprenoids” refers to a large and diverse class of naturally-occurring organic chemicals similar to terpenes. Terpenoids are derived from five-carbon isoprene units assembled and modified in a variety of ways, and are classified in groups based on the number of isoprene units used in group members. Hemiterpenoids have one isoprene unit. Monoterpenoids have two isoprene units. Sesquiterpenoids have three isoprene units. Diterpenoids have four isoprene units. Sesterterpenoids have five isoprene units. Triterpenoids have six isoprene units. Tetraterpenoids have eight isoprene units. Polyterpenoids have more than eight isoprene units.
As used herein, the term “headspace” refers to the vapor/air mixture trapped above a solid or liquid sample in a sealed vessel.
As used herein, the singular terms “a,” “an,” and “the” include the plural reference unless the context clearly indicates otherwise.
Unless otherwise indicated, nucleic acids are written left to right in 5′ to 3′ orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively.
It is intended that every maximum numerical limitation given throughout this specification includes every lower numerical limitation, as if such lower numerical limitations were expressly written herein. Every minimum numerical limitation given throughout this specification will include every higher numerical limitation, as if such higher numerical limitations were expressly written herein. Every numerical range given throughout this specification will include every narrower numerical range that falls within such broader numerical range, as if such narrower numerical ranges were all expressly written herein.
Reference to “about” a value or parameter herein also includes (and describes) embodiments that are directed to that value or parameter per se.
It is understood that all aspects and embodiments of the invention described herein include “comprising,” “consisting,” and “consisting essentially of” aspects and embodiments. It is to be understood that methods or compositions “consisting essentially of” the recited elements include only the specified steps or materials and those that do not materially affect the basic and novel characteristics of those methods and compositions.
It is to be understood that this invention is not limited to the particular methodology, protocols, and reagents described, as these may vary, depending upon the context they are used by those of skill in the art.
DETAILED DESCRIPTION OF THE INVENTION
Isoprene monomer is employed in the manufacture of polyisoprene and various copolymers (with isobutylene, butadiene, styrene, or other monomers). Building a strain (prokaryotic or eukaryotic) capable of producing commercially viable levels of isoprene requires optimization of part of or the entire DXP or MVA pathway or both MVA and DXP pathways. A key enzyme in the pathway is isoprene synthase (IspS), which converts the precursor DMAPP to isoprene. Isoprene synthases (IspS) that have been identified include those from plants such as poplar, English oak and kudzu vine. Some of the plant IspS enzymes identified have been partially characterized in part by expression in E. coli and some of the kinetic parameters of these enzymes have been determined in vitro with purified protein. However, the kinetic parameters (Km, rate, etc.) of the native IspS enzymes are insufficient for commercial production of isoprene in a biological host. Thus, one problem to be solved is the provision of isoprene synthase variants (e.g. with substitutions at specific residues) which have improved properties such that a greater amount of isoprene can be biologically produced.
To solve this problem as described herein, an isoprene synthase may be expressed in a host (e.g. a bacterial host). In addition, isoprene synthase variants are engineered for a change in a property of interest. Characterization of IspS variants is accomplished via any means or test suitable and is preferably based on the assessment of properties of interest. These variants are useful for the commercial production of isoprene in a biological host.
Properties of interest include, but are not limited to: increased intracellular activity, specific productivity of cells expressing IspS (g/L/OD/hr) (described in greater detail below), yield (g/g glucose) (e.g., Equation 2 below), and cellular performance index (grams of isoprene/gram of dry cell weight). Without being bound by theory, these properties can be achieved by one or a combination of any of the following properties of IspS: increased cellular viability (e.g., better growth of host cells due to relief of substrate (e.g., DMAPP) toxicity or reduced isoprene synthase enzyme toxicity to the host cell), increased kcat, decreased Km, increased specific activity, increased solubility, decreased insolubility, improved ribosome binding, increased translation initiation rate, increased translation elongation rate, increased transcription initiation rate, increased transcription elongation rate, decreased secondary structure of DNA, decreased secondary structure of RNA, increased secondary structure of DNA, increased secondary structure of RNA, increased folding rates, increased affinity for intracellular chaperones, increased stability, decreased protein turnover, decreased exposure to intracellular protease, decreased affinity for intracellular protease, decreased localization to the periplasm, improved localization to the cytoplasm, decreased inclusion body formation, decreased membrane localization, increased expression due to a more favorable codon, increased DNA stability, increased RNA stability, and decreased RNA degradation. In brief, any mutation that has a positive effect on the properties of nucleic acid sequences (DNA and RNA) encoding or expressing the IspS variant, or the biochemical properties of the IspS enzyme itself, could allow for greater activity within the cell. Other properties of interest include pH optima, temperature stability (e.g., Tm value), as well as sensitivity to potential inhibitors including substrate or product inhibition. Oxidative and proteolytic stability are also of interest. Furthermore, activation or inhibition due to metal ion effects and ionic strength is of interest.
These properties and parameters can be assessed by the conversion of DMAPP to isoprene in vitro with purified or partially purified isoprene synthase or in vivo in the context of a host organism such as E. coli expressing the DXP pathway, the MVA pathway, or both. It is contemplated that enzymes having various degrees of stability, solubility, activity, and/or expression level in one or more of test conditions will find use in the present invention for the production of isoprene in a diversity of hosts.
The invention features compositions and methods for the production of increased amounts of isoprene. In particular, these compositions and methods may increase the rate of isoprene production and the total amount of isoprene that is produced. The biosynthetic processes for isoprene production described herein are a desirable alternative to using natural rubber. As discussed further below, the amount of isoprene produced by cells can be greatly increased by introducing a heterologous nucleic acid encoding an isoprene synthase (IspS) variant into the cells.
Additionally, isoprene production by cells containing a heterologous isoprene synthase nucleic acid can be enhanced by increasing the amount of one or more DXP pathway polypeptides (e.g., a 1-deoxy-D-xylulose-5-phosphate synthase (DXS) polypeptide) and/or an isopentenyl diphosphate isomerase (IDI) polypeptide, expressed by the cells. For example, a DXS nucleic acid and/or an IDI nucleic acid can be introduced into the cells. The DXS nucleic acid may be a heterologous nucleic acid or a duplicate copy of an endogenous nucleic acid. Similarly, the IDI nucleic acid may be a heterologous nucleic acid or a duplicate copy of an endogenous nucleic acid. In some embodiments, the amount of DXS and/or IDI polypeptide is increased by replacing the endogenous DXS and/or IDI promoters or regulatory regions with other promoters and/or regulatory regions that result in greater transcription of the DXS and/or IDI nucleic acids. In some embodiments, the cells contain both a heterologous nucleic acid encoding an isoprene synthase polypeptide (e.g., a plant isoprene synthase nucleic acid) and a duplicate copy of an endogenous nucleic acid encoding an isoprene synthase polypeptide.
The encoded DXS and IDI polypeptides are part of the DXP pathway for the biosynthesis of isoprene (FIG. 22). DXS polypeptides convert pyruvate and D-glyceraldehyde-3-phosphate into 1-deoxy-D-xylulose-5-phosphate. While not intending to be bound by any particular theory, it is believed that increasing the amount of DXS polypeptide increases the flow of carbon through the DXP pathway, leading to greater isoprene production. IDI polypeptides catalyze the interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). While not intending to be bound by any particular theory, it is believed that increasing the amount of IDI polypeptide in cells increases the amount of IPP that is converted into DMAPP, which in turn is converted into isoprene.
As is further detailed below, in some embodiments, the production of isoprene by cells containing a heterologous isoprene synthase nucleic acid can be augmented by increasing expression of one or more MVA polypeptide(s) in the cells (FIG. 22). Exemplary MVA pathways polypeptides include any of the following polypeptides: acetyl-CoA acetyltransferase (AA-CoA thiolase) polypeptides, 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) polypeptides, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) polypeptides, mevalonate kinase (MVK) polypeptides, phosphomevalonate kinase (PMK) polypeptides, diphosphomevalonte decarboxylase (MVD) polypeptides, IDI polypeptides, and polypeptides (e.g., fusion polypeptides) having an activity of two or more MVA pathway polypeptides. For example, one or more MVA pathway nucleic acids can be introduced into the cells. In some embodiments, the cells contain the upper MVA pathway, which includes AA-CoA thiolase, HMG-CoA synthase, and HMG-CoA reductase nucleic acids. In some embodiments, the cells contain the lower MVA pathway, which includes MVK, PMK, MVD, and IDI nucleic acids. In some embodiments, the cells contain the entire MVA pathway, which includes AA-CoA thiolase, HMG-CoA synthase, HMG-CoA reductase, MVK, PMK, MVD, and IDI nucleic acids. The MVA pathway nucleic acids may be heterologous nucleic acids or duplicate copies of endogenous nucleic acids. In some embodiments, the amount of one or more MVA pathway polypeptides is increased by replacing the endogenous promoters or regulatory regions for the MVA pathway nucleic acids with other promoters and/or regulatory regions that result in greater transcription of the MVA pathway nucleic acids. In some embodiments, the cells contain both a heterologous nucleic acid encoding an isoprene synthase polypeptide (e.g., a plant isoprene synthase nucleic acid) and a duplicate copy of an endogenous nucleic acid encoding an isoprene synthase polypeptide.
In some embodiments, at least a portion of the cells maintain the heterologous isoprene synthase, DXS, IDI, other DXP pathway and/or MVA pathway nucleic acids for at least about 5, 10, 20, 50, 75, 100, 200, 300, or more cell divisions in a continuous culture (such as a continuous culture without dilution). In some embodiments of any of the aspects of the invention, the nucleic acid comprising the heterologous or duplicate copy of an endogenous isoprene synthase, DXS, IDI, other DXP pathway and/or MVA pathway nucleic acids also comprises a selective marker, such as a kanamycin, ampicillin, carbenicillin, gentamicin, hygromycin, phleomycin, bleomycin, neomycin, or chloramphenicol antibiotic resistance nucleic acid
Isoprene synthase polypeptides convert dimethylallyl diphosphate (DMAPP) into isoprene. Exemplary isoprene synthase polypeptides include polypeptides, fragments of polypeptides, peptides, and fusions polypeptides that have at least one activity of an isoprene synthase polypeptide. Standard methods can be used to determine whether a polypeptide has isoprene synthase polypeptide activity by measuring the ability of the polypeptide to convert DMAPP into isoprene in vitro, in a cell extract, or in vivo. Isoprene synthase polypeptide activity in the cell extract can be measured, for example, as described in Silver et al., J. Biol. Chem. 270:13010-13016, 1995 and references therein.
In one embodiment, DMAPP (Sigma) is evaporated to dryness under a stream of nitrogen and rehydrated to a concentration of 100 mM in 100 mM potassium phosphate buffer pH 8.2 and stored at −20° C. To perform the assay, a solution of 5 μl of 1M MgCl2, 1 mM (250 μg/ml) DMAPP, 65 μl of Plant Extract Buffer (PEB) (50 mM Tris-HCl, pH 8.0, 20 mM MgCl2, 5% glycerol, and 2 mM DTT) is added to 25 μl of cell extract in a 20 ml Headspace vial with a metal screw cap and teflon coated silicon septum (Agilent Technologies) and cultured at 37° C. for 15 minutes with shaking. The reaction is quenched by adding 200 μl of 250 mM EDTA or by heat inactivation, and isoprene is quantified by GC/MS.
Isoprene Synthase Parent Sequences
Isoprene synthase variants may be generated from a parent isoprene synthase, wherein the parent isoprene synthase may be an isoprene synthase as described herein, including wild type and non-wild type isoprene synthases. Exemplary parent isoprene synthase nucleic acids include nucleic acids that encode a polypeptide, fragment of a polypeptide, peptide, or fusion polypeptide that has at least one activity of an isoprene synthase polypeptide. Exemplary parent isoprene synthase polypeptides and nucleic acids include naturally-occurring polypeptides and nucleic acids from any of the source organisms described herein as well as variant polypeptides and nucleic acids derived from any of the source organisms described herein.
In some embodiments, the parent isoprene synthase is from the family Fabaceae, the family Salicaceae, or the family Fagaceae. In some embodiments, the parent isoprene synthase polypeptide or nucleic acid is a naturally-occurring polypeptide or nucleic acid from Pueraria montana (kudzu) (Sharkey et al., Plant Physiology 137: 700-712, 2005), poplar (such as Populus alba x tremula CAC35696, Miller et al., Planta 213: 483-487, 2001) or Populus alba, aspen (such as Populus tremuloides) Silver et al., JBC 270(22): 13010-1316, I995), or English Oak (Quercus robur) (Zimmer et al., WO 98/02550). Suitable parent isoprene synthases include, but are not limited to, those identified by GenBank Accession Nos. AY341431, AY316691, AB198180, AJ294819.1, EU693027.1, EF638224.1, AM410988.1, EF147555.1, AY279379, AJ457070, and AY182241. Additional parent sequences are described in PCT/US2009/041581 (WO 2009/132220) and PCT/US2010/032134 (WO 2010/124146).
In various embodiments, the parent isoprene synthase has at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 92%, at least about 94%, at least about 96%, at least about 98%, at least about 99% sequence identity with MEA P. alba. In other embodiments, the parent isoprene synthase has at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 92%, at least about 94%, at least about 96%, at least about 98%, at least about 99% sequence identity with full-length P. alba or complete P. alba (see, e.g., FIGS. 21A and 21B).
Several methods are known in the art that are suitable for generating variants of the enzymes of the present invention, including but not limited to site-saturation mutagenesis, scanning mutagenesis, insertional mutagenesis, random mutagenesis, site-directed mutagenesis, and directed-evolution, as well as various other recombinatorial approaches.
Isoprene Synthase Variants
Polypeptides, e.g. isoprene synthase, have a three-dimensional structure determined by the primary amino acid sequence and the environment surrounding the polypeptide. This three-dimensional structure establishes the polypeptide's activity, stability, binding affinity, binding specificity, and other biochemical attributes. Thus, knowledge of a protein's three-dimensional structure can provide much guidance in designing improvements to its biological activity; for example, greater catalytic activity and/or solubility. Crystal structure coordinates for various isoprene synthases are described in WO2009/132220 and PCT/US2010/032134 (WO 2010/124146).
The inventors have identified residue locations, the mutation (e.g., substitution) of which in a parent isoprene synthase may result in one or more improved properties in the variant. In one aspect of the invention, the mutation is a substitution at a location corresponding to the position in MEA P. alba isoprene synthase (SEQ ID NO:1) as denoted below.











Location
Definition







N-term
Residues 1-215


Hinge Region
Residues 216-245


C-term
Residues 246-544


N-term helices
Residues 134-179


Dimer Interface
Residues 240-255 and 316-353


Substrate
Residues 441-454 and 515-527


Capture Loops


Active Site
Residues within 8 Å of active site based on PDB 3N0G


Buried
Surface Accessibility below 35% and located internally


Surface
Residues located on the surface of the structure


Surface Loop
Residues in loops located on the surface of the structure









As such, in one aspect, isoprene variants with one or more substitution(s) in these locations (e.g., as described in Examples 7-9) can be made and screened for improved isoprene synthase properties, which are described in greater detail below. Exemplary properties that one of skill can use for identification purposes include, but are not limited to, increased specific activity as compared to specific activity of MEA P. alba isoprene synthase and growth of a recombinant host cell in which the isoprene synthase variant is being expressed as compared to the growth a recombinant host cell expressing MEA P. alba isoprene synthase under similar growth conditions. In one aspect, substitutions can be made in the N-terminal helices or at residues that would have some interaction with residues in the N-terminal helices such that it improves isoprene synthase properties.
The sequence of MEA P. alba isoprene synthase is as follows (and is also shown in FIG. 20):







(SEQ ID NO: 1)







MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEK


 


AEFLTLLELIDNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGT


 


ALSFRLLRQHGFEVSQEAFSGFKDQNGNFLENLKEDIKAILSLYEASFLA


 


LEGENILDEAKVFAISHLKELSEEKIGKELAEQVNHALELPLHRRTQRLE


 


AVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRETSRWWRRVGLA


 


TKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFSFVTIIDDIYDVY


 


GTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDK


 


GENILPYLTKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQ


 


LVFAYFAVVQNIKKEEIENLQKYHDTISRPSHIFRLCNDLASASAEIARG


 


ETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKEKLGGSLFAKPFV


 


ETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPILPFER.






Combinations of the substitutions described herein can be made and screened for improved isoprene synthase properties. Non-limiting examples of isoprene synthase variants with combinations of substitutions that have one or more improved isoprene synthase properties are described in Table 25 (Variants 1-27) and Table 26 (Variants 1-12).
In one aspect, residues identified for mutation of MEA P. alba include those listed in Table A. Residues and residue numbering in Table A corresponds to the residue numbering of the MEA P. alba shown in SEQ ID NO:1 (FIG. 20).







TABLE A







Mutagenesis Sites











Position
Residue
Selection Criteria















3
A
surface hydrophobic



7
A
symmetry contact



9
Y
symmetry contact



12
N
Conservation



13
S
Conservation



16
Y
N-terminal loop



18
Y
N-terminal loop



20
L
Conservation



23
D
Conservation



25
D
surface hydrophilic



26
E
symmetry contact



27
S
surface hydrophilic



33
D
symmetry contact



36
K
symmetry contact



44
R
symmetry contact



50
K
surface hydrophilic



53
F
Conservation



59
L
surface hydrophobic



69
G
Conservation



74
S
surface hydrophilic



78
G
Conservation



81
D
surface hydrophilic



87
G
surface hydrophobic



99
G
Conservation



116
Q
Conservation



117
E
symmetry contact



120
S
surface hydrophilic



121
G
surface loop



125
Q
surface hydrophilic



127
G
Conservation



139
A
Conservation



165
I
surface hydrophobic



173
E
surface hydrophilic



174
E
symmetry contact



177
G
Conservation



179
E
surface hydrophilic



194
R
Conservation



197
Q
Conservation



202
V
Conservation



216
Q
Conservation



240
T
Conservation



246
R
symmetry contact



251
T
surface hydrophilic



254
H
Conservation



287
F
active site



290
V
active site



308
L
surface hydrophobic



376
L
flexible loops



377
Y
symmetry contact



379
K
Conservation



389
G
Conservation



397
G
active site



400
Q
hydrophobic pocket



403
F
active site



421
Q
Conservation



426
T
Conservation



430
P
Misc



434
F
active site



445
A
surface hydrophobic



448
A
Conservation



457
S
flexible loops



462
T
Conservation



476
N
surface hydrophilic



487
K
surface loop



488
E
surface loop



489
K
surface loop



490
L
surface loop



491
G
surface loop



492
G
surface loop



493
S
surface loop



495
F
surface loop



496
A
surface loop



497
K
surface loop



498
P
Conservation



509
Q
Conservation



514
Y
active site



521
T
Conservation



539
I
C-terminal



540
L
surface hydrophobic



544
R
Conservation










As used in Table A, “surface hydrophobic” indicates a hydrophobic amino acid residue present on the surface of the protein, “surface hydrophilic” indicates a hydrophilic amino acid residue present on the surface of the protein, “symmetry contact” indicates a residue on the surface of the isoprene synthase molecule which contacts another isoprene synthase molecule in the crystal structure, “conservation” indicates a residue common to most terpene synthases, “N-terminal loop” indicates a residue present in the N-terminal loop of the protein, “surface loop” indicates a residue present in a surface loop of the protein, “active site” indicates a residue present in the active site of the protein, “flexible loop” indicates a residue present in a flexible loop region of the protein, “hydrophobic pocket” indicates a residue present in the hydrophobic pocket of the protein, and “C-terminal” indicates a residue present in the C-terminal region of the protein.
As further described in the Examples below, various isoprene synthase variants were made with substitutions at the residue locations shown in Table A. Any of the variants described herein (including in Tables A, B, the claims, or the Examples) may be used in the compositions and methods of the invention. Specific substitutions generated for MEA P. alba variants are shown in Table B.







TABLE B





Exemplary Amino Acid Substitutions for MEA P. alba
























A003C
A003D
A003E
A003F
A003G
A003H
A003I
A003K
A003L
A003M


A003N
A003P
A003Q
A003R
A003S
A003T
A003V
A003W
A003Y
A007C


A007D
A007E
A007F
A007G
A007H
A007I
A007K
A007L
A007M
A007N


A007P
A007Q
A007R
A007S
A007T
A007V
A007W
A007Y
Y009A
Y009C


Y009D
Y009E
Y009F
Y009G
Y009H
Y009I
Y009K
Y009L
Y009M
Y009N


Y009P
Y009Q
Y009R
Y009S
Y009T
Y009V
Y009W
N012A
N012C
N012D


N012E
N012F
N012G
N012H
N012I
N012K
N012L
N012M
N012P
N012Q


N012R
N012S
N012T
N012V
N012W
N012Y
S013A
S013C
S013D
S013E


S013F
S013G
S013H
S013I
S013K
S013L
S013M
S013N
S01P3
S013Q


S013R
S013T
S013V
S013W
S013Y
Y016A
Y016C
Y016D
Y016E
Y016F


Y016G
Y016H
Y016I
Y016K
Y016L
Y016M
Y016N
Y016P
Y016Q
Y016R


Y016S
Y016T
Y016V
Y016W
Y018A
Y018C
Y018D
Y0E18
Y018F
Y018G


Y018H
Y018I
Y018K
Y018L
Y018M
Y018N
Y018P
Y018Q
Y018R
Y018S


Y018T
Y018V
Y018W
L020A
L020C
L020D
L020E
L020F
L020G
L020H


L020I
L020K
L020M
L020N
L020P
L020Q
L020R
L020S
L020T
L020V


L020W
L020Y
D023A
D023C
D023E
D023F
D023G
D023H
D023I
D023K


D023L
D023M
D023N
D023P
D023Q
D023R
D023S
D023T
D023V
D023W


D023Y
D025A
D025C
D025E
D025F
D025G
D025H
D025I
D025K
D025L


D025M
D025N
D025P
D025Q
D025R
D025S
D025T
D025V
D025W
D025Y


E026A
E026C
E026D
E026F
E026G
E026H
E026I
E026K
E026L
E026M


E026N
E026P
E026Q
E026R
E026S
E026T
E026V
E026W
E026Y
S027A


S027C
S027D
S027E
S027F
S027G
S027H
S027I
S027K
S027L
S027M


S027N
S027P
S027Q
S0R27
S027V
S027W
S027Y
D033A
D033C
D033E


D033F
D033G
D033H
D033I
D033K
D033L
D033M
D033N
D033P
D033Q


D033R
D033S
D033T
D033V
D033W
D033Y
K036A
K036C
K036D
K036E


K036F
K036G
K036G
K036I
K036L
K036M
K036N
K036P
K036Q
K036R


K036S
K036T
K036V
K036W
K036Y
R044A
R044C
R044D
R044E
R044F


R044G
R044H
R044I
R044K
R044L
R044M
R044N
R044P
R044Q
R044S


R044T
R044V
R044W
R044Y
K050A
K050C
K050D
K050E
K050F
K050G


K050H
K050I
K050L
K050M
K050N
K050P
K050Q
K050R
K050S
K050T


K050V
K050W
K050Y
F053A
F053C
F053D
F053E
F053G
F053H
F053I


F053K
F053L
F053M
F053N
F053P
F053Q
F053R
F053S
F053T
F053V


F053W
F053Y
L059A
L059C
L059D
L059E
L059F
L059G
L059H
L059I


L059K
L059M
L059N
L059P
L059Q
L059R
L059S
L059T
L059V
L059W


L059Y
G069A
G069C
G069D
G069E
G069F
G069H
G069I
G069K
G069L


G069M
G069N
G069P
G069Q
G069R
G069S
G069T
G069V
G069W
G069Y


S074A
S074C
S074D
S074E
S074F
S074G
S074H
S074I
S074K
S074L


S074M
S074N
S074P
S074Q
S074R
S074T
S074V
S074W
S074Y
G078A


G078C
G078D
G078E
G078F
G078H
G078I
G078K
G078L
G078M
G078N


G078P
G078Q
G078R
G078S
G078T
G078V
G078W
G078Y
D081A
D081C


D081E
D081F
D081G
D081H
D081I
D081K
D081L
D081M
D081N
D081P


D081Q
D081R
D081S
D081T
D081V
D081W
D081Y
G087A
G087C
G087D


G087E
G087F
G087H
G087I
G087K
G087L
G087M
G087N
G087P
G087Q


G087R
G087S
G087T
G087V
G087W
G087Y
G099A
G099C
G099D
G099E


G099F
G099H
G099I
G099K
G099L
G099M
G099N
G099P
G099Q
G099R


G099S
G099T
G099V
G099W
G099Y
Q116A
Q116C
Q116D
Q116E
Q116F


Q116G
Q116H
Q116I
Q116K
Q116L
Q116M
Q116N
Q116P
Q116R
Q116S


Q116T
Q116V
Q116W
Q116Y
E117A
E117C
E117D
E117F
E117G
E117H


E117I
E117K
E117L
E117M
E117N
E117P
E117Q
E117R
E117S
E117T


E117V
E117W
E117Y
S120A
S120C
S120D
S120E
S120F
S120G
S120H


S120I
S120K
S120L
S120M
S120N
S120P
S120Q
S120R
S120T
S120V


S120W
S120Y
G121A
G121C
G121D
G121E
G121F
G121H
G121I
G121K


G121L
G121M
G121N
G121P
G121Q
G121R
G121S
G121T
G121V
G121W


G121Y
Q125A
Q125C
Q125D
Q125E
Q125F
Q125G
Q125H
Q125I
Q125K


Q125L
Q125M
Q125N
Q125P
Q125R
Q125S
Q125T
Q125V
Q125W
Q125Y


G127A
G127C
G127D
G127E
G127F
G127H
G127I
G127K
G127L
G127M


G127N
G127P
G127Q
G127R
G127S
G127T
G127V
G127W
G127Y
A139C


A139D
A139E
A139F
A139G
A139H
A139I
A139K
A139L
A139M
A139N


A139P
A139Q
A139R
A139S
A139T
A139V
A139W
A139Y
I165A
I165C


I165D
I165E
I165F
I165G
I165H
I165K
I165L
I165M
I165N
I165P


I165Q
I165R
I165S
I165T
I165V
I165W
I165Y
E173A
E173C
E173D


E173F
E173G
E173H
E173I
E173K
E173L
E173M
E173N
E173P
E173Q


E173R
E173S
E173T
E173V
E173W
E173Y
E174A
E174C
E174D
E174F


E174G
E174H
E174I
E174K
E174L
E174M
E174N
E174P
E174Q
E174R


E174S
E174T
E174V
E174W
E174Y
G177A
G177C
G177D
G177E
G177F


G177H
G177I
G177K
G177L
G177M
G177N
G177P
G177Q
G177R
G177S


G177T
G177V
G177W
G177Y
E179A
E179C
E179D
E179F
E179G
E179H


E179I
E179K
E179L
E179M
E179N
E179P
E179Q
E179R
E179S
E179T


E179V
E179W
E179Y
R194A
R194C
R194D
R194E
R194F
R194G
R194H


R194I
R194K
R194L
R194M
R194N
R194P
R194Q
R194S
R194T
R194V


R194W
R194Y
Q197A
Q197C
Q197D
Q197E
Q197F
Q197G
Q197H
Q197I


Q197K
Q197L
Q197M
Q197N
Q197P
Q197R
Q197S
Q197T
Q197V
Q197W


Q197Y
V202A
V202C
V202D
V202E
V202F
V202G
V202H
V202I
V202K


V202L
V202M
V202N
V202P
V202Q
V202R
V202S
V202T
V202W
V202Y


Q216A
Q216C
Q216D
Q216E
Q216F
Q216G
Q216H
Q216I
Q216K
Q216L


Q216M
Q216N
Q216P
Q216R
Q216S
Q216T
Q216V
Q216W
Q216Y
T240A


T240C
T240D
T240E
T240F
T240G
T240H
T240I
T240K
T240L
T240M


T240N
T240P
T240Q
T240R
T240S
T240V
T240W
T240Y
R246A
R246C


R246D
R246E
R246F
R246G
R246H
R246I
R246K
R246L
R246M
R246N


R246P
R246Q
R246S
R246T
R246V
R246W
R246Y
T251A
T251C
T251D


T251E
T251F
T251G
T251H
T251I
T251K
T251L
T251M
T251N
T251P


T251Q
T251R
T251S
T251V
T251W
T251Y
H254A
H254C
H254D
H254E


H254F
H254G
H254I
H254K
H254L
H254M
H254N
H254P
H254Q
H254R


H254S
H254T
H254V
H254W
H254Y
F287A
F287C
F287D
F287E
F287G


F287H
F287I
F287K
F287L
F287M
F287N
F287P
F287Q
F287R
F287S


F287T
F287V
F287W
F287Y
V290A
V290C
V290D
V290E
V290F
V290G


V290H
V290I
V290K
V290L
V290M
V290N
V290P
V290Q
V290R
V290S


V290T
V290W
V290Y
L308A
L308C
L308D
L308E
L308F
L308G
L308H


L308I
L308K
L308M
L308N
L308P
L308Q
L308R
L308S
L308T
L308V


L308W
L308Y
L376A
L376C
L376D
L376E
L376F
L376G
L376H
L376I


L376K
L376M
L376N
L376P
L376Q
L376R
L376S
L376T
L376V
L376W


L376Y
Y377A
Y377C
Y377D
Y377E
Y377F
Y377G
Y377H
Y377I
Y377K


Y377L
Y377M
Y377N
Y377P
Y377Q
Y377R
Y377S
Y377T
Y377V
Y377W


K379A
K379C
K379D
K379E
K379F
K379G
K379H
K379I
K379L
K379M


K379N
K379P
K379Q
K379R
K379S
K379T
K379V
K379W
K379Y
G389A


G389C
G389D
G389E
G389F
G389H
G389I
G389K
G389L
G389M
G389N


G389P
G389Q
G389R
G389S
G389T
G389V
G389W
G389Y
G397A
G397C


G397D
G397E
G397F
G397H
G397I
G397K
G397L
G397M
G397N
G397P


G397Q
G397R
G397S
G397T
G397V
G397W
G397Y
Q400A
Q400C
Q400D


Q400E
Q400F
Q400G
Q400H
Q400I
Q400K
Q400L
Q400M
Q400N
Q400P


Q400R
Q400S
Q400T
Q400V
Q400W
Q400Y
F403A
F403C
F403D
F403E


F403G
F403H
F403I
F403K
F403L
F403M
F403N
F403P
F403Q
F403R


F403S
F403T
F403V
F403W
F403Y
Q421A
Q421C
Q421D
Q421E
Q421F


Q421G
Q421H
Q421I
Q421K
Q421L
Q421M
Q421N
Q421P
Q421R
Q421S


Q421T
Q421V
Q421W
Q421Y
T426A
T426C
T426D
T426E
T426F
T426G


T426H
T426I
T426K
T426L
T426M
T426N
T426P
T426Q
T426R
T426S


T426V
T426W
T426Y
P430A
P430C
P430D
P430E
P430F
P430G
P430H


P430I
P430K
P430L
P430M
P430N
P430Q
P430R
P430S
P430T
P430V


P430W
P430Y
F434A
F434C
F434D
F434E
F434G
F434H
F434I
F434K


F434L
F434M
F434N
F434P
F434Q
F434R
F434S
F434T
F434V
F434W


F434Y
A445C
A445D
A445E
A445F
A445G
A445H
A445I
A445K
A445L


A445M
A445N
A445P
A445Q
A445R
A445S
A445T
A445V
A445W
A445Y


A448C
A448D
A448E
A448F
A448G
A448H
A448I
A448K
A448L
A448M


A448N
A448P
A448Q
A448R
A448S
A448T
A448V
A448W
A448Y
S457A


S457C
S457D
S457E
S457F
S457G
S457H
S457I
S457K
S457L
S457M


S457N
S457P
S457Q
S457R
S457T
S457V
S457W
S457Y
T462A
T462C


T462D
T462E
T462F
T462G
T462H
T462I
T462K
T462L
T462M
T462N


T462P
T462Q
T462R
T462S
T462V
T462W
T462Y
N476A
N476C
N476D


N476E
N476F
N476G
N476H
N476I
N476K
N476L
N476M
N476P
N476Q


N476R
N476S
N476T
N476V
N476W
N476Y
K487A
K487C
K487D
K487E


K487F
K487G
K487H
K487I
K487L
K487M
K487N
K487P
K487Q
K487R


K487S
K487T
K487V
K487W
K487Y
E488A
E488C
E488D
E488F
E488G


E488H
E488I
E488K
E488L
E488M
E488N
E488P
E488Q
E488R
E488S


E488T
E488V
E488W
E488Y
K489A
K489C
K489D
K489E
K489F
K489G


K489H
K489I
K489L
K489M
K489N
K489P
K489Q
K489R
K489S
K489T


K489V
K489W
K489Y
L490A
L490C
L490D
L490E
L490F
L490G
L490H


L490I
L490K
L490M
L490N
L490P
L490Q
L490R
L490S
L490T
L490V


L490W
L490Y
G491A
G491C
G491D
G491E
G491F
G491H
G491I
G491K


G491L
G491M
G491N
G491P
G491Q
G491R
G491S
G491T
G491V
G491W


G491Y
G492A
G492C
G492D
G492E
G492F
G492H
G492I
G492K
G492L


G492M
G492N
G492P
G492Q
G492R
G492S
G492T
G492V
G492W
G492Y


S493A
S493C
S493D
S493E
S493F
S493G
S493H
S493I
S493K
S493L


S493M
S493N
S493P
S493Q
S493R
S493T
S493V
S493W
S493Y
F495A


F495C
F495D
F495E
F495G
F495H
F495I
F495K
F495L
F495M
F495N


F495P
F495Q
F495R
F495S
F495T
F495V
F495W
F495Y
A496C
A496D


A496E
A496F
A496G
A496H
A496I
A496K
A496L
A496M
A496N
A496P


A496Q
A496R
A496S
A496T
A496V
A496W
A496Y
K497A
K497C
K497D


K497E
K497F
K497G
K497H
K497I
K497L
K497M
K497N
K497P
K497Q


K497R
K497S
K497T
K497V
K497W
K497Y
P498A
P498C
P498D
P498E


P498F
P498G
P498H
P498I
P498K
P498L
P498M
P498N
P498Q
P498R


P498S
P498T
P498V
P498W
P498Y
Q509A
Q509C
Q509D
Q509E
Q509F


Q509G
Q509H
Q509I
Q509K
Q509L
Q509M
Q509N
Q509P
Q509R
Q509S


Q509T
Q509V
Q509W
Q509Y
Y514A
Y514C
Y514D
Y514E
Y514F
Y514G


Y514H
Y514I
Y514K
Y514L
Y514M
Y514N
Y514P
Y514Q
Y514R
Y514S


Y514T
Y514V
Y514W
T521A
T521C
T521D
T521E
T521F
T521G
T521H


T521I
T521K
T521L
T521M
T521N
T521P
T521Q
T521R
T521S
T521V


T521W
T521Y
I539A
I539C
I539D
I539E
I539F
I539G
I539H
I539K


I539L
I539M
I539N
I539P
I539Q
I539R
I539S
I539T
I539V
I539W


I539Y
L540A
L540C
L540D
L540E
L540F
L540G
L540H
L540I
L540K


L540M
L540N
L540P
L540Q
L540R
L540S
L540T
L540V
L540W
L540Y


R544A
R544C
R544D
R544E
R544F
R544G
R544H
R544I
R544K
R544L


R544M
R544N
R544P
R544Q
R544S
R544T
R544V
R544W
R544Y









Table B describes specific substitutions in MEA P. alba. Corresponding residues in other parent isoprene synthases may be similarly mutated to generate isoprene synthase variants of the invention. Table C shows exemplary substitutions of the invention, wherein “X” refers to any amino acid residue. However, it is to be understood that “X” refers to an amino acid residue other than the amino acid residue resulting from the substitution (e.g., for “X3C”, X is an amino acid residue other than C). Residue numbering in Table C corresponds to that of the MEA P. alba sequence. Accordingly, it is to be understood that residue “003” for a different parent sequence (e.g. wild type P. tremuloides) indicates the residue in the P. tremuloides sequence which corresponds to residue 003 of MEA P. alba, and that residue “003” of the P. tremuloides sequence is not necessarily the third sequential residue in the amino acid sequence of P. tremuloides.







TABLE C





Exemplary amino acid residue substitutions
























X003C
X003D
X003E
X003F
X003G
X003H
X003I
X003K
X003L
X003M


X003N
X003P
X003Q
X003R
X003S
X003T
X003V
X003W
X003Y
X007C


X007D
X007E
X007F
X007G
X007H
X007I
X007K
X007L
X007M
X007N


X007P
X007Q
X007R
X007S
X007T
X007V
X007W
X007Y
X009A
X009C


X009D
X009E
X009F
X009G
X009H
X009I
X009K
X009L
X009M
X009N


X009P
X009Q
X009R
X009S
X009T
X009V
X009W
X012A
X012C
X012D


X012E
X012F
X012G
X012H
X012I
X012K
X012L
X012M
X012P
X012Q


X012R
X012S
X012T
X012V
X012W
X012Y
X013A
X013C
X013D
X013E


X013F
X013G
X013H
X013I
X013K
X013L
X013M
X013N
X01P3
X013Q


X013R
X013T
X013V
X013W
X013Y
X016A
X016C
X016D
X016E
X016F


X016G
X016H
X016I
X016K
X016L
X016M
X016N
X016P
X016Q
X016R


X016S
X016T
X016V
X016W
X018A
X018C
X018D
X0E18
X018F
X018G


X018H
X018I
X018K
X018L
X018M
X018N
X018P
X018Q
X018R
X018S


X018T
X018V
X018W
X020A
X020C
X020D
X020E
X020F
X020G
X020H


X020I
X020K
X020M
X020N
X020P
X020Q
X020R
X020S
X020T
X020V


X020W
X020Y
X023A
X023C
X023E
X023F
X023G
X023H
X023I
X023K


X023L
X023M
X023N
X023P
X023Q
X023R
X023S
X023T
X023V
X023W


X023Y
X025A
X025C
X025E
X025F
X025G
X025H
X025I
X025K
X025L


X025M
X025N
X025P
X025Q
X025R
X025S
X025T
X025V
X025W
X025Y


X026A
X026C
X026D
X026F
X026G
X026H
X026I
X026K
X026L
X026M


X026N
X026P
X026Q
X026R
X026S
X026T
X026V
X026W
X026Y
X027A


X027C
X027D
X027E
X027F
X027G
X027H
X027I
X027K
X027L
X027M


X027N
X027P
X027Q
X0R27
X027V
X027W
X027Y
X033A
X033C
X033E


X033F
X033G
X033H
X033I
X033K
X033L
X033M
X033N
X033P
X033Q


X033R
X033S
X033T
X033V
X033W
X033Y
X036A
X036C
X036D
X036E


X036F
X036G
X036G
X036I
X036L
X036M
X036N
X036P
X036Q
X036R


X036S
X036T
X036V
X036W
X036Y
X044A
X044C
X044D
X044E
X044F


X044G
X044H
X044I
X044K
X044L
X044M
X044N
X044P
X044Q
X044S


X044T
X044V
X044W
X044Y
X050A
X050C
X050D
X050E
X050F
X050G


X050H
X050I
X050L
X050M
X050N
X050P
X050Q
X050R
X050S
X050T


X050V
X050W
X050Y
X053A
X053C
X053D
X053E
X053G
X053H
X053I


X053K
X053L
X053M
X053N
X053P
X053Q
X053R
X053S
X053T
X053V


X053W
X053Y
X059A
X059C
X059D
X059E
X059F
X059G
X059H
X059I


X059K
X059M
X059N
X059P
X059Q
X059R
X059S
X059T
X059V
X059W


X059Y
X069A
X069C
X069D
X069E
X069F
X069H
X069I
X069K
X069L


X069M
X069N
X069P
X069Q
X069R
X069S
X069T
X069V
X069W
X069Y


X074A
X074C
X074D
X074E
X074F
X074G
X074H
X074I
X074K
X074L


X074M
X074N
X074P
X074Q
X074R
X074T
X074V
X074W
X074Y
X078A


X078C
X078D
X078E
X078F
X078H
X078I
X078K
X078L
X078M
X078N


X078P
X078Q
X078R
X078S
X078T
X078V
X078W
X078Y
X081A
X081C


X081E
X081F
X081G
X081H
X081I
X081K
X081L
X081M
X081N
X081P


X081Q
X081R
X081S
X081T
X081V
X081W
X081Y
X087A
X087C
X087D


X087E
X087F
X087H
X087I
X087K
X087L
X087M
X087N
X087P
X087Q


X087R
X087S
X087T
X087V
X087W
X087Y
X099A
X099C
X099D
X099E


X099F
X099H
X099I
X099K
X099L
X099M
X099N
X099P
X099Q
X099R


X099S
X099T
X099V
X099W
X099Y
X116A
X116C
X116D
X116E
X116F


X116G
X116H
X116I
X116K
X116L
X116M
X116N
X116P
X116R
X116S


X116T
X116V
X116W
X116Y
X117A
X117C
X117D
X117F
X117G
X117H


X117I
X117K
X117L
X117M
X117N
X117P
X117Q
X117R
X117S
X117T


X117V
X117W
X117Y
X120A
X120C
X120D
X120E
X120F
X120G
X120H


X120I
X120K
X120L
X120M
X120N
X120P
X120Q
X120R
X120T
X120V


X120W
X120Y
X121A
X121C
X121D
X121E
X121F
X121H
X121I
X121K


X121L
X121M
X121N
X121P
X121Q
X121R
X121S
X121T
X121V
X121W


X121Y
X125A
X125C
X125D
X125E
X125F
X125G
X125H
X125I
X125K


X125L
X125M
X125N
X125P
X125R
X125S
X125T
X125V
X125W
X125Y


X127A
X127C
X127D
X127E
X127F
X127H
X127I
X127K
X127L
X127M


X127N
X127P
X127Q
X127R
X127S
X127T
X127V
X127W
X127Y
X139C


X139D
X139E
X139F
X139G
X139H
X139I
X139K
X139L
X139M
X139N


X139P
X139Q
X139R
X139S
X139T
X139V
X139W
X139Y
X165A
X165C


X165D
X165E
X165F
X165G
X165H
X165K
X165L
X165M
X165N
X165P


X165Q
X165R
X165S
X165T
X165V
X165W
X165Y
X173A
X173C
X173D


X173F
X173G
X173H
X173I
X173K
X173L
X173M
X173N
X173P
X173Q


X173R
X173S
X173T
X173V
X173W
X173Y
X174A
X174C
X174D
X174F


X174G
X174H
X174I
X174K
X174L
X174M
X174N
X174P
X174Q
X174R


X174S
X174T
X174V
X174W
X174Y
X177A
X177C
X177D
X177E
X177F


X177H
X177I
X177K
X177L
X177M
X177N
X177P
X177Q
X177R
X177S


X177T
X177V
X177W
X177Y
X179A
X179C
X179D
X179F
X179G
X179H


X179I
X179K
X179L
X179M
X179N
X179P
X179Q
X179R
X179S
X179T


X179V
X179W
X179Y
X194A
X194C
X194D
X194E
X194F
X194G
X194H


X194I
X194K
X194L
X194M
X194N
X194P
X194Q
X194S
X194T
X194V


X194W
X194Y
X197A
X197C
X197D
X197E
X197F
X197G
X197H
X197I


X197K
X197L
X197M
X197N
X197P
X197R
X197S
X197T
X197V
X197W


X197Y
X202A
X202C
X202D
X202E
X202F
X202G
X202H
X202I
X202K


X202L
X202M
X202N
X202P
X202Q
X202R
X202S
X202T
X202W
X202Y


X216A
X216C
X216D
X216E
X216F
X216G
X216H
X216I
X216K
X216L


X216M
X216N
X216P
X216R
X216S
X216T
X216V
X216W
X216Y
X240A


X240C
X240D
X240E
X240F
X240G
X240H
X240I
X240K
X240L
X240M


X240N
X240P
X240Q
X240R
X240S
X240V
X240W
X240Y
X246A
X246C


X246D
X246E
X246F
X246G
X246H
X246I
X246K
X246L
X246M
X246N


X246P
X246Q
X246S
X246T
X246V
X246W
X246Y
X251A
X251C
X251D


X251E
X251F
X251G
X251H
X251I
X251K
X251L
X251M
X251N
X251P


X251Q
X251R
X251S
X251V
X251W
X251Y
X254A
X254C
X254D
X254E


X254F
X254G
X254I
X254K
X254L
X254M
X254N
X254P
X254Q
X254R


X254S
X254T
X254V
X254W
X254Y
X287A
X287C
X287D
X287E
X287G


X287H
X287I
X287K
X287L
X287M
X287N
X287P
X287Q
X287R
X287S


X287T
X287V
X287W
X287Y
X290A
X290C
X290D
X290E
X290F
X290G


X290H
X290I
X290K
X290L
X290M
X290N
X290P
X290Q
X290R
X290S


X290T
X290W
X290Y
X308A
X308C
X308D
X308E
X308F
X308G
X308H


X308I
X308K
X308M
X308N
X308P
X308Q
X308R
X308S
X308T
X308V


X308W
X308Y
X376A
X376C
X376D
X376E
X376F
X376G
X376H
X376I


X376K
X376M
X376N
X376P
X376Q
X376R
X376S
X376T
X376V
X376W


X376Y
X377A
X377C
X377D
X377E
X377F
X377G
X377H
X377I
X377K


X377L
X377M
X377N
X377P
X377Q
X377R
X377S
X377T
X377V
X377W


X379A
X379C
X379D
X379E
X379F
X379G
X379H
X379I
X379L
X379M


X379N
X379P
X379Q
X379R
X379S
X379T
X379V
X379W
X379Y
X389A


X389C
X389D
X389E
X389F
X389H
X389I
X389K
X389L
X389M
X389N


X389P
X389Q
X389R
X389S
X389T
X389V
X389W
X389Y
X397A
X397C


X397D
X397E
X397F
X397H
X397I
X397K
X397L
X397M
X397N
X397P


X397Q
X397R
X397S
X397T
X397V
X397W
X397Y
X400A
X400C
X400D


X400E
X400F
X400G
X400H
X400I
X400K
X400L
X400M
X400N
X400P


X400R
X400S
X400T
X400V
X400W
X400Y
X403A
X403C
X403D
X403E


X403G
X403H
X403I
X403K
X403L
X403M
X403N
X403P
X403Q
X403R


X403S
X403T
X403V
X403W
X403Y
X421A
X421C
X421D
X421E
X421F


X421G
X421H
X421I
X421K
X421L
X421M
X421N
X421P
X421R
X421S


X421T
X421V
X421W
X421Y
X426A
X426C
X426D
X426E
X426F
X426G


X426H
X426I
X426K
X426L
X426M
X426N
X426P
X426Q
X426R
X426S


X426V
X426W
X426Y
X430A
X430C
X430D
X430E
X430F
X430G
X430H


X430I
X430K
X430L
X430M
X430N
X430Q
X430R
X430S
X430T
X430V


X430W
X430Y
X434A
X434C
X434D
X434E
X434G
X434H
X434I
X434K


X434L
X434M
X434N
X434P
X434Q
X434R
X434S
X434T
X434V
X434W


X434Y
X445C
X445D
X445E
X445F
X445G
X445H
X445I
X445K
X445L


X445M
X445N
X445P
X445Q
X445R
X445S
X445T
X445V
X445W
X445Y


X448C
X448D
X448E
X448F
X448G
X448H
X448I
X448K
X448L
X448M


X448N
X448P
X448Q
X448R
X448S
X448T
X448V
X448W
X448Y
X457A


X457C
X457D
X457E
X457F
X457G
X457H
X457I
X457K
X457L
X457M


X457N
X457P
X457Q
X457R
X457T
X457V
X457W
X457Y
X462A
X462C


X462D
X462E
X462F
X462G
X462H
X462I
X462K
X462L
X462M
X462N


X462P
X462Q
X462R
X462S
X462V
X462W
X462Y
X476A
X476C
X476D


X476E
X476F
X476G
X476H
X476I
X476K
X476L
X476M
X476P
X476Q


X476R
X476S
X476T
X476V
X476W
X476Y
X487A
X487C
X487D
X487E


X487F
X487G
X487H
X487I
X487L
X487M
X487N
X487P
X487Q
X487R


X487S
X487T
X487V
X487W
X487Y
X488A
X488C
X488D
X488F
X488G


X488H
X488I
X488K
X488L
X488M
X488N
X488P
X488Q
X488R
X488S


X488T
X488V
X488W
X488Y
X489A
X489C
X489D
X489E
X489F
X489G


X489H
X489I
X489L
X489M
X489N
X489P
X489Q
X489R
X489S
X489T


X489V
X489W
X489Y
X490A
X490C
X490D
X490E
X490F
X490G
X490H


X490I
X490K
X490M
X490N
X490P
X490Q
X490R
X490S
X490T
X490V


X490W
X490Y
X491A
X491C
X491D
X491E
X491F
X491H
X491I
X491K


X491L
X491M
X491N
X491P
X491Q
X491R
X491S
X491T
X491V
X491W


X491Y
X492A
X492C
X492D
X492E
X492F
X492H
X492I
X492K
X492L


X492M
X492N
X492P
X492Q
X492R
X492S
X492T
X492V
X492W
X492Y


X493A
X493C
X493D
X493E
X493F
X493G
X493H
X493I
X493K
X493L


X493M
X493N
X493P
X493Q
X493R
X493T
X493V
X493W
X493Y
X495A


X495C
X495D
X495E
X495G
X495H
X495I
X495K
X495L
X495M
X495N


X495P
X495Q
X495R
X495S
X495T
X495V
X495W
X495Y
X496C
X496D


X496E
X496F
X496G
X496H
X496I
X496K
X496L
X496M
X496N
X496P


X496Q
X496R
X496S
X496T
X496V
X496W
X496Y
X497A
X497C
X497D


X497E
X497F
X497G
X497H
X497I
X497L
X497M
X497N
X497P
X497Q


X497R
X497S
X497T
X497V
X497W
X497Y
X498A
X498C
X498D
X498E


X498F
X498G
X498H
X498I
X498K
X498L
X498M
X498N
X498Q
X498R


X498S
X498T
X498V
X498W
X498Y
X509A
X509C
X509D
X509E
X509F


X509G
X509H
X509I
X509K
X509L
X509M
X509N
X509P
X509R
X509S


X509T
X509V
X509W
X509Y
X514A
X514C
X514D
X514E
X514F
X514G


X514H
X514I
X514K
X514L
X514M
X514N
X514P
X514Q
X514R
X514S


X514T
X514V
X514W
X521A
X521C
X521D
X521E
X521F
X521G
X521H


X521I
X521K
X521L
X521M
X521N
X521P
X521Q
X521R
X521S
X521V


X521W
X521Y
X539A
X539C
X539D
X539E
X539F
X539G
X539H
X539K


X539L
X539M
X539N
X539P
X539Q
X539R
X539S
X539T
X539V
X539W


X539Y
X540A
X540C
X540D
X540E
X540F
X540G
X540H
X540I
X540K


X540M
X540N
X540P
X540Q
X540R
X540S
X540T
X540V
X540W
X540Y


X544A
X544C
X544D
X544E
X544F
X544G
X544H
X544I
X544K
X544L


X544M
X544N
X544P
X544Q
X544S
X544T
X544V
X544W
X544Y









In some embodiments, the variant comprises a substitution selected from the group consisting of: X003T, X013L, X165Y, X421R, X495L, X509T, and X540V. In some embodiments, the variant comprises X003T. In some embodiments, the variant comprises X013L. In some embodiments, the variant comprises X165Y. In some embodiments, the variant comprises X421R. In some embodiments, the variant comprises X495L. In some embodiments, the variant comprises X509T. In some embodiments, the variant comprises X540V. In some embodiments, the variant comprises the substitution X491S. In some embodiments, the variant further comprises the substitution X491S, in addition to another substitution selected from the group consisting of: X003C, X003D, X003E, X003F, X003G, X003H, X003I, X003K, X003L, X003M, X003N, X003P, X003Q, X003R, X003S, X003T, X003V, X003W, X003Y, X007C, X007D, X007E, X007F, X007G, X007H, X007I, X007K, X007L, X007M, X007N, X007P, X007Q, X007R, X007S, X007T, X007V, X007W, X007Y, X009A, X009C, X009D, X009E, X009F, X009G, X009H, X009I, X009K, X009L, X009M, X009N, X009P, X009Q, X009R, X009S, X009T, X009V, X009W, X012A, X012C, X012D, X012E, X012F, X012G, X012H, X012I, X012K, X012L, X012M, X012P, X012Q, X012R, X012S, X012T, X012V, X012W, X012Y, X013A, X013C, X013D, X013E, X013F, X013G, X013H, X013I, X013K, X013L, X013M, X013N, X01P3, X013Q, X013R, X013T, X013V, X013W, X013Y, X016A, X016C, X016D, X016E, X016F, X016G, X016H, X016I, X016K, X016L, X016M, X016N, X016P, X016Q, X016R, X016S, X016T, X016V, X016W, X018A, X018C, X018D, X0E18, X018F, X018G, X018H, X018I, X018K, X018L, X018M, X018N, X018P, X018Q, X018R, X018S, X018T, X018V, X018W, X020A, X020C, X020D, X020E, X020F, X020G, X020H, X020I, X020K, X020M, X020N, X020P, X020Q, X020R, X020S, X020T, X020V, X020W, X020Y, X023A, X023C, X023E, X023F, X023G, X023H, X023I, X023K, X023L, X023M, X023N, X023P, X023Q, X023R, X023S, X023T, X023V, X023W, X023Y, X025A, X025C, X025E, X025F, X025G, X025H, X025I, X025K, X025L, X025M, X025N, X025P, X025Q, X025R, X025S, X025T, X025V, X025W, X025Y, X026A, X026C, X026D, X026F, X026G, X026H, X026I, X026K, X026L, X026M, X026N, X026P, X026Q, X026R, X026S, X026T, X026V, X026W, X026Y, X027A, X027C, X027D, X027E, X027F, X027G, X027H, X027I, X027K, X027L, X027M, X027N, X027P, X027Q, X0R27, X027V, X027W, X027Y, X033A, X033C, X033E, X033F, X033G, X033H, X033I, X033K, X033L, X033M, X033N, X033P, X033Q, X033R, X033S, X033T, X033V, X033W, X033Y, X036A, X036C, X036D, X036E, X036F, X036G, X036G, X036I, X036L, X036M, X036N, X036P, X036Q, X036R, X036S, X036T, X036V, X036W, X036Y, X044A, X044C, X044D, X044E, X044F, X044G, X044H, X044I, X044K, X044L, X044M, X044N, X044P, X044Q, X044S, X044T, X044V, X044W, X044Y, X050A, X050C, X050D, X050E, X050F, X050G, X050H, X050I, X050L, X050M, X050N, X050P, X050Q, X050R, X050S, X050T, X050V, X050W, X050Y, X053A, X053C, X053D, X053E, X053G, X053H, X053I, X053K, X053L, X053M, X053N, X053P, X053Q, X053R, X053S, X053T, X053V, X053W, X053Y, X059A, X059C, X059D, X059E, X059F, X059G, X059H, X059I, X059K, X059M, X059N, X059P, X059Q, X059R, X059S, X059T, X059V, X059W, X059Y, X069A, X069C, X069D, X069E, X069F, X069H, X069I, X069K, X069L, X069M, X069N, X069P, X069Q, X069R, X069S, X069T, X069V, X069W, X069Y, X074A, X074C, X074D, X074E, X074F, X074G, X074H, X074I, X074K, X074L, X074M, X074N, X074P, X074Q, X074R, X074T, X074V, X074W, X074Y, X078A, X078C, X078D, X078E, X078F, X078H, X078I, X078K, X078L, X078M, X078N, X078P, X078Q, X078R, X078S, X078T, X078V, X078W, X078Y, X081A, X081C, X081E, X081F, X081G, X081H, X081I, X081K, X081L, X081M, X081N, X081P, X081Q, X081R, X081S, X081T, X081V, X081W, X081Y, X087A, X087C, X087D, X087E, X087F, X087H, X087I, X087K, X087L, X087M, X087N, X087P, X087Q, X087R, X087S, X087T, X087V, X087W, X087Y, X099A, X099C, X099D, X099E, X099F, X099H, X099I, X099K, X099L, X099M, X099N, X099P, X099Q, X099R, X099S, X099T, X099V, X099W, X099Y, X116A, X116C, X116D, X116E, X116F, X116G, X116H, X116I, X116K, X116L, X116M, X116N, X116P, X116R, X116S, X116T, X116V, X116W, X116Y, X117A, X117C, X117D, X117F, X117G, X117H, X117I, X117K, X117L, X117M, X117N, X117P, X117Q, X117R, X117S, X117T, X117V, X117W, X117Y, X120A, X120C, X120D, X120E, X120F, X120G, X120H, X120I, X120K, X120L, X120M, X120N, X120P, X120Q, X120R, X120T, X120V, X120W, X120Y, X121A, X121C, X121D, X121E, X121F, X121H, X121I, X121K, X121L, X121M, X121N, X121P, X121Q, X121R, X121S, X121T, X121V, X121W, X121Y, X125A, X125C, X125D, X125E, X125F, X125G, X125H, X125I, X125K, X125L, X125M, X125N, X125P, X125R, X125S, X125T, X125V, X125W, X125Y, X127A, X127C, X127D, X127E, X127F, X127H, X127I, X127K, X127L, X127M, X127N, X127P, X127Q, X127R, X127S, X127T, X127V, X127W, X127Y, X139C, X139D, X139E, X139F, X139G, X139H, X139I, X139K, X139L, X139M, X139N, X139P, X139Q, X139R, X139S, X139T, X139V, X139W, X139Y, X165A, X165C, X165D, X165E, X165F, X165G, X165H, X165K, X165L, X165M, X165N, X165P, X165Q, X165R, X165S, X165T, X165V, X165W, X165Y, X173A, X173C, X173D, X173F, X173G, X173H, X173I, X173K, X173L, X173M, X173N, X173P, X173Q, X173R, X173S, X173T, X173V, X173W, X173Y, X174A, X174C, X174D, X174F, X174G, X174H, X174I, X174K, X174L, X174M, X174N, X174P, X174Q, X174R, X174S, X174T, X174V, X174W, X174Y, X177A, X177C, X177D, X177E, X177F, X177H, X177I, X177K, X177L, X177M, X177N, X177P, X177Q, X177R, X177S, X177T, X177V, X177W, X177Y, X179A, X179C, X179D, X179F, X179G, X179H, X179I, X179K, X179L, X179M, X179N, X179P, X179Q, X179R, X179S, X179T, X179V, X179W, X179Y, X194A, X194C, X194D, X194E, X194F, X194G, X194H, X194I, X194K, X194L, X194M, X194N, X194P, X194Q, X194S, X194T, X194V, X194W, X194Y, X197A, X197C, X197D, X197E, X197F, X197G, X197H, X197I, X197K, X197L, X197M, X197N, X197P, X197R, X197S, X197T, X197V, X197W, X197Y, X202A, X202C, X202D, X202E, X202F, X202G, X202H, X202I, X202K, X202L, X202M, X202N, X202P, X202Q, X202R, X202S, X202T, X202W, X202Y, X216A, X216C, X216D, X216E, X216F, X216G, X216H, X216I, X216K, X216L, X216M, X216N, X216P, X216R, X216S, X216T, X216V, X216W, X216Y, X240A, X240C, X240D, X240E, X240F, X240G, X240H, X240I, X240K, X240L, X240M, X240N, X240P, X240Q, X240R, X240S, X240V, X240W, X240Y, X246A, X246C, X246D, X246E, X246F, X246G, X246H, X246I, X246K, X246L, X246M, X246N, X246P, X246Q, X246S, X246T, X246V, X246W, X246Y, X251A, X251C, X251D, X251E, X251F, X251G, X251H, X251I, X251K, X251L, X251M, X251N, X251P, X251Q, X251R, X251S, X251V, X251W, X251Y, X254A, X254C, X254D, X254E, X254F, X254G, X254I, X254K, X254L, X254M, X254N, X254P, X254Q, X254R, X254S, X254T, X254V, X254W, X254Y, X287A, X287C, X287D, X287E, X287G, X287H, X287I, X287K, X287L, X287M, X287N, X287P, X287Q, X287R, X287S, X287T, X287V, X287W, X287Y, X290A, X290C, X290D, X290E, X290F, X290G, X290H, X290I, X290K, X290L, X290M, X290N, X290P, X290Q, X290R, X290S, X290T, X290W, X290Y, X308A, X308C, X308D, X308E, X308F, X308G, X308H, X308I, X308K, X308M, X308N, X308P, X308Q, X308R, X308S, X308T, X308V, X308W, X308Y, X376A, X376C, X376D, X376E, X376F, X376G, X376H, X376I, X376K, X376M, X376N, X376P, X376Q, X376R, X376S, X376T, X376V, X376W, X376Y, X377A, X377C, X377D, X377E, X377F, X377G, X377H, X377I, X377K, X377L, X377M, X377N, X377P, X377Q, X377R, X377S, X377T, X377V, X377W, X379A, X379C, X379D, X379E, X379F, X379G, X379H, X379I, X379L, X379M, X379N, X379P, X379Q, X379R, X379S, X379T, X379V, X379W, X379Y, X389A, X389C, X389D, X389E, X389F, X389H, X389I, X389K, X389L, X389M, X389N, X389P, X389Q, X389R, X389S, X389T, X389V, X389W, X389Y, X397A, X397C, X397D, X397E, X397F, X397H, X397I, X397K, X397L, X397M, X397N, X397P, X397Q, X397R, X397S, X397T, X397V, X397W, X397Y, X400A, X400C, X400D, X400E, X400F, X400G, X400H, X400I, X400K, X400L, X400M, X400N, X400P, X400R, X400S, X400T, X400V, X400W, X400Y, X403A, X403C, X403D, X403E, X403G, X403H, X403I, X403K, X403L, X403M, X403N, X403P, X403Q, X403R, X403S, X403T, X403V, X403W, X403Y, X421A, X421C, X421D, X421E, X421F, X421G, X421H, X421I, X421K, X421L, X421M, X421N, X421P, X421R, X421S, X421T, X421V, X421W, X421Y, X426A, X426C, X426D, X426E, X426F, X426G, X426H, X426I, X426K, X426L, X426M, X426N, X426P, X426Q, X426R, X426S, X426V, X426W, X426Y, X430A, X430C, X430D, X430E, X430F, X430G, X430H, X430I, X430K, X430L, X430M, X430N, X430Q, X430R, X430S, X430T, X430V, X430W, X430Y, X434A, X434C, X434D, X434E, X434G, X434H, X434I, X434K, X434L, X434M, X434N, X434P, X434Q, X434R, X434S, X434T, X434V, X434W, X434Y, X445C, X445D, X445E, X445F, X445G, X445H, X445I, X445K, X445L, X445M, X445N, X445P, X445Q, X445R, X445S, X445T, X445V, X445W, X445Y, X448C, X448D, X448E, X448F, X448G, X448H, X448I, X448K, X448L, X448M, X448N, X448P, X448Q, X448R, X448S, X448T, X448V, X448W, X448Y, X457A, X457C, X457D, X457E, X457F, X457G, X457H, X457I, X457K, X457L, X457M, X457N, X457P, X457Q, X457R, X457T, X457V, X457W, X457Y, X462A, X462C, X462D, X462E, X462F, X462G, X462H, X462I, X462K, X462L, X462M, X462N, X462P, X462Q, X462R, X462S, X462V, X462W, X462Y, X476A, X476C, X476D, X476E, X476F, X476G, X476H, X476I, X476K, X476L, X476M, X476P, X476Q, X476R, X476S, X476T, X476V, X476W, X476Y, X487A, X487C, X487D, X487E, X487F, X487G, X487H, X487I, X487L, X487M, X487N, X487P, X487Q, X487R, X487S, X487T, X487V, X487W, X487Y, X488A, X488C, X488D, X488F, X488G, X488H, X488I, X488K, X488L, X488M, X488N, X488P, X488Q, X488R, X488S, X488T, X488V, X488W, X488Y, X489A, X489C, X489D, X489E, X489F, X489G, X489H, X489I, X489L, X489M, X489N, X489P, X489Q, X489R, X489S, X489T, X489V, X489W, X489Y, X490A, X490C, X490D, X490E, X490F, X490G, X490H, X490I, X490K, X490M, X490N, X490P, X490Q, X490R, X490S, X490T, X490V, X490W, X490Y, X491A, X491C, X491D, X491E, X491F, X491H, X491I, X491K, X491L, X491M, X491N, X491P, X491Q, X491R, X491S, X491T, X491V, X491W, X491Y, X492A, X492C, X492D, X492E, X492F, X492H, X492I, X492K, X492L, X492M, X492N, X492P, X492Q, X492R, X492S, X492T, X492V, X492W, X492Y, X493A, X493C, X493D, X493E, X493F, X493G, X493H, X493I, X493K, X493L, X493M, X493N, X493P, X493Q, X493R, X493T, X493V, X493W, X493Y, X495A, X495C, X495D, X495E, X495G, X495H, X495I, X495K, X495L, X495M, X495N, X495P, X495Q, X495R, X495S, X495T, X495V, X495W, X495Y, X496C, X496D, X496E, X496F, X496G, X496H, X496I, X496K, X496L, X496M, X496N, X496P, X496Q, X496R, X496S, X496T, X496V, X496W, X496Y, X497A, X497C, X497D, X497E, X497F, X497G, X497H, X497I, X497L, X497M, X497N, X497P, X497Q, X497R, X497S, X497T, X497V, X497W, X497Y, X498A, X498C, X498D, X498E, X498F, X498G, X498H, X498I, X498K, X498L, X498M, X498N, X498Q, X498R, X498S, X498T, X498V, X498W, X498Y, X509A, X509C, X509D, X509E, X509F, X509G, X509H, X509I, X509K, X509L, X509M, X509N, X509P, X509R, X509S, X509T, X509V, X509W, X509Y, X514A, X514C, X514D, X514E, X514F, X514G, X514H, X514I, X514K, X514L, X514M, X514N, X514P, X514Q, X514R, X514S, X514T, X514V, X514W, X521A, X521C, X521D, X521E, X521F, X521G, X521H, X521I, X521K, X521L, X521M, X521N, X521P, X521Q, X521R, X521S, X521V, X521W, X521Y, X539A, X539C, X539D, X539E, X539F, X539G, X539H, X539K, X539L, X539M, X539N, X539P, X539Q, X539R, X539S, X539T, X539V, X539W, X539Y, X540A, X540C, X540D, X540E, X540F, X540G, X540H, X540I, X540K, X540M, X540N, X540P, X540Q, X540R, X540S, X540T, X540V, X540W, X540Y, X544A, X544C, X544D, X544E, X544F, X544G, X544H, X544I, X544K, X544L, X544M, X544N, X544P, X544Q, X544S, X544T, X544V, X544W, and X544Y; wherein X represents any amino acid; and wherein each amino acid residue position is numbered by correspondence with an amino acid residue position in the P. alba isoprene synthase sequence as shown in FIG. 20. The substitution X491S may be combined with any other substitution described in Tables B or C.
In some embodiments, the variant comprises the amino acid residues: N438, E451, and Y514. In some embodiments, the variant comprises the amino acid residues: F287, G397, N438, E451, and Y514. In some embodiments, the variant comprises S491. In some embodiments, the variant comprises S491 in addition to a substitution selected from the group consisting of: X003C, X003D, X003E, X003F, X003G, X003H, X003I, X003K, X003L, X003M, X003N, X003P, X003Q, X003R, X003S, X003T, X003V, X003W, X003Y, X007C, X007D, X007E, X007F, X007G, X007H, X007I, X007K, X007L, X007M, X007N, X007P, X007Q, X007R, X007S, X007T, X007V, X007W, X007Y, X009A, X009C, X009D, X009E, X009F, X009G, X009H, X009I, X009K, X009L, X009M, X009N, X009P, X009Q, X009R, X009S, X009T, X009V, X009W, X012A, X012C, X012D, X012E, X012F, X012G, X012H, X012I, X012K, X012L, X012M, X012P, X012Q, X012R, X012S, X012T, X012V, X012W, X012Y, X013A, X013C, X013D, X013E, X013F, X013G, X013H, X013I, X013K, X013L, X013M, X013N, X01P3, X013Q, X013R, X013T, X013V, X013W, X013Y, X016A, X016C, X016D, X016E, X016F, X016G, X016H, X016I, X016K, X016L, X016M, X016N, X016P, X016Q, X016R, X016S, X016T, X016V, X016W, X018A, X018C, X018D, X0E18, X018F, X018G, X018H, X018I, X018K, X018L, X018M, X018N, X018P, X018Q, X018R, X018S, X018T, X018V, X018W, X020A, X020C, X020D, X020E, X020F, X020G, X020H, X020I, X020K, X020M, X020N, X020P, X020Q, X020R, X020S, X020T, X020V, X020W, X020Y, X023A, X023C, X023E, X023F, X023G, X023H, X023I, X023K, X023L, X023M, X023N, X023P, X023Q, X023R, X023S, X023T, X023V, X023W, X023Y, X025A, X025C, X025E, X025F, X025G, X025H, X025I, X025K, X025L, X025M, X025N, X025P, X025Q, X025R, X025S, X025T, X025V, X025W, X025Y, X026A, X026C, X026D, X026F, X026G, X026H, X026I, X026K, X026L, X026M, X026N, X026P, X026Q, X026R, X026S, X026T, X026V, X026W, X026Y, X027A, X027C, X027D, X027E, X027F, X027G, X027H, X027I, X027K, X027L, X027M, X027N, X027P, X027Q, X0R27, X027V, X027W, X027Y, X033A, X033C, X033E, X033F, X033G, X033H, X033I, X033K, X033L, X033M, X033N, X033P, X033Q, X033R, X033S, X033T, X033V, X033W, X033Y, X036A, X036C, X036D, X036E, X036F, X036G, X036G, X036I, X036L, X036M, X036N, X036P, X036Q, X036R, X036S, X036T, X036V, X036W, X036Y, X044A, X044C, X044D, X044E, X044F, X044G, X044H, X044I, X044K, X044L, X044M, X044N, X044P, X044Q, X044S, X044T, X044V, X044W, X044Y, X050A, X050C, X050D, X050E, X050F, X050G, X050H, X050I, X050L, X050M, X050N, X050P, X050Q, X050R, X050S, X050T, X050V, X050W, X050Y, X053A, X053C, X053D, X053E, X053G, X053H, X053I, X053K, X053L, X053M, X053N, X053P, X053Q, X053R, X053S, X053T, X053V, X053W, X053Y, X059A, X059C, X059D, X059E, X059F, X059G, X059H, X059I, X059K, X059M, X059N, X059P, X059Q, X059R, X059S, X059T, X059V, X059W, X059Y, X069A, X069C, X069D, X069E, X069F, X069H, X069I, X069K, X069L, X069M, X069N, X069P, X069Q, X069R, X069S, X069T, X069V, X069W, X069Y, X074A, X074C, X074D, X074E, X074F, X074G, X074H, X074I, X074K, X074L, X074M, X074N, X074P, X074Q, X074R, X074T, X074V, X074W, X074Y, X078A, X078C, X078D, X078E, X078F, X078H, X078I, X078K, X078L, X078M, X078N, X078P, X078Q, X078R, X078S, X078T, X078V, X078W, X078Y, X081A, X081C, X081E, X081F, X081G, X081H, X081I, X081K, X081L, X081M, X081N, X081P, X081Q, X081R, X081S, X081T, X081V, X081W, X081Y, X087A, X087C, X087D, X087E, X087F, X087H, X087I, X087K, X087L, X087M, X087N, X087P, X087Q, X087R, X087S, X087T, X087V, X087W, X087Y, X099A, X099C, X099D, X099E, X099F, X099H, X099I, X099K, X099L, X099M, X099N, X099P, X099Q, X099R, X099S, X099T, X099V, X099W, X099Y, X116A, X116C, X116D, X116E, X116F, X116G, X116H, X116I, X116K, X116L, X116M, X116N, X116P, X116R, X116S, X116T, X116V, X116W, X116Y, X117A, X117C, X117D, X117F, X117G, X117H, X117I, X117K, X117L, X117M, X117N, X117P, X117Q, X117R, X117S, X117T, X117V, X117W, X117Y, X120A, X120C, X120D, X120E, X120F, X120G, X120H, X120I, X120K, X120L, X120M, X120N, X120P, X120Q, X120R, X120T, X120V, X120W, X120Y, X121A, X121C, X121D, X121E, X121F, X121H, X121I, X121K, X121L, X121M, X121N, X121P, X121Q, X121R, X121S, X121T, X121V, X121W, X121Y, X125A, X125C, X125D, X125E, X125F, X125G, X125H, X125I, X125K, X125L, X125M, X125N, X125P, X125R, X125S, X125T, X125V, X125W, X125Y, X127A, X127C, X127D, X127E, X127F, X127H, X127I, X127K, X127L, X127M, X127N, X127P, X127Q, X127R, X127S, X127T, X127V, X127W, X127Y, X139C, X139D, X139E, X139F, X139G, X139H, X139I, X139K, X139L, X139M, X139N, X139P, X139Q, X139R, X139S, X139T, X139V, X139W, X139Y, X165A, X165C, X165D, X165E, X165F, X165G, X165H, X165K, X165L, X165M, X165N, X165P, X165Q, X165R, X165S, X165T, X165V, X165W, X165Y, X173A, X173C, X173D, X173F, X173G, X173H, X173I, X173K, X173L, X173M, X173N, X173P, X173Q, X173R, X173S, X173T, X173V, X173W, X173Y, X174A, X174C, X174D, X174F, X174G, X174H, X174I, X174K, X174L, X174M, X174N, X174P, X174Q, X174R, X174S, X174T, X174V, X174W, X174Y, X177A, X177C, X177D, X177E, X177F, X177H, X177I, X177K, X177L, X177M, X177N, X177P, X177Q, X177R, X177S, X177T, X177V, X177W, X177Y, X179A, X179C, X179D, X179F, X179G, X179H, X179I, X179K, X179L, X179M, X179N, X179P, X179Q, X179R, X179S, X179T, X179V, X179W, X179Y, X194A, X194C, X194D, X194E, X194F, X194G, X194H, X194I, X194K, X194L, X194M, X194N, X194P, X194Q, X194S, X194T, X194V, X194W, X194Y, X197A, X197C, X197D, X197E, X197F, X197G, X197H, X197I, X197K, X197L, X197M, X197N, X197P, X197R, X197S, X197T, X197V, X197W, X197Y, X202A, X202C, X202D, X202E, X202F, X202G, X202H, X202I, X202K, X202L, X202M, X202N, X202P, X202Q, X202R, X202S, X202T, X202W, X202Y, X216A, X216C, X216D, X216E, X216F, X216G, X216H, X216I, X216K, X216L, X216M, X216N, X216P, X216R, X216S, X216T, X216V, X216W, X216Y, X240A, X240C, X240D, X240E, X240F, X240G, X240H, X240I, X240K, X240L, X240M, X240N, X240P, X240Q, X240R, X240S, X240V, X240W, X240Y, X246A, X246C, X246D, X246E, X246F, X246G, X246H, X246I, X246K, X246L, X246M, X246N, X246P, X246Q, X246S, X246T, X246V, X246W, X246Y, X251A, X251C, X251D, X251E, X251F, X251G, X251H, X251I, X251K, X251L, X251M, X251N, X251P, X251Q, X251R, X251S, X251V, X251W, X251Y, X254A, X254C, X254D, X254E, X254F, X254G, X254I, X254K, X254L, X254M, X254N, X254P, X254Q, X254R, X254S, X254T, X254V, X254W, X254Y, X287A, X287C, X287D, X287E, X287G, X287H, X287I, X287K, X287L, X287M, X287N, X287P, X287Q, X287R, X287S, X287T, X287V, X287W, X287Y, X290A, X290C, X290D, X290E, X290F, X290G, X290H, X290I, X290K, X290L, X290M, X290N, X290P, X290Q, X290R, X290S, X290T, X290W, X290Y, X308A, X308C, X308D, X308E, X308F, X308G, X308H, X308I, X308K, X308M, X308N, X308P, X308Q, X308R, X308S, X308T, X308V, X308W, X308Y, X376A, X376C, X376D, X376E, X376F, X376G, X376H, X376I, X376K, X376M, X376N, X376P, X376Q, X376R, X376S, X376T, X376V, X376W, X376Y, X377A, X377C, X377D, X377E, X377F, X377G, X377H, X377I, X377K, X377L, X377M, X377N, X377P, X377Q, X377R, X377S, X377T, X377V, X377W, X379A, X379C, X379D, X379E, X379F, X379G, X379H, X379I, X379L, X379M, X379N, X379P, X379Q, X379R, X379S, X379T, X379V, X379W, X379Y, X389A, X389C, X389D, X389E, X389F, X389H, X389I, X389K, X389L, X389M, X389N, X389P, X389Q, X389R, X389S, X389T, X389V, X389W, X389Y, X397A, X397C, X397D, X397E, X397F, X397H, X397I, X397K, X397L, X397M, X397N, X397P, X397Q, X397R, X397S, X397T, X397V, X397W, X397Y, X400A, X400C, X400D, X400E, X400F, X400G, X400H, X400I, X400K, X400L, X400M, X400N, X400P, X400R, X400S, X400T, X400V, X400W, X400Y, X403A, X403C, X403D, X403E, X403G, X403H, X403I, X403K, X403L, X403M, X403N, X403P, X403Q, X403R, X403S, X403T, X403V, X403W, X403Y, X421A, X421C, X421D, X421E, X421F, X421G, X421H, X421I, X421K, X421L, X421M, X421N, X421P, X421R, X421S, X421T, X421V, X421W, X421Y, X426A, X426C, X426D, X426E, X426F, X426G, X426H, X426I, X426K, X426L, X426M, X426N, X426P, X426Q, X426R, X426S, X426V, X426W, X426Y, X430A, X430C, X430D, X430E, X430F, X430G, X430H, X430I, X430K, X430L, X430M, X430N, X430Q, X430R, X430S, X430T, X430V, X430W, X430Y, X434A, X434C, X434D, X434E, X434G, X434H, X434I, X434K, X434L, X434M, X434N, X434P, X434Q, X434R, X434S, X434T, X434V, X434W, X434Y, X445C, X445D, X445E, X445F, X445G, X445H, X445I, X445K, X445L, X445M, X445N, X445P, X445Q, X445R, X445S, X445T, X445V, X445W, X445Y, X448C, X448D, X448E, X448F, X448G, X448H, X448I, X448K, X448L, X448M, X448N, X448P, X448Q, X448R, X448S, X448T, X448V, X448W, X448Y, X457A, X457C, X457D, X457E, X457F, X457G, X457H, X457I, X457K, X457L, X457M, X457N, X457P, X457Q, X457R, X457T, X457V, X457W, X457Y, X462A, X462C, X462D, X462E, X462F, X462G, X462H, X462I, X462K, X462L, X462M, X462N, X462P, X462Q, X462R, X462S, X462V, X462W, X462Y, X476A, X476C, X476D, X476E, X476F, X476G, X476H, X476I, X476K, X476L, X476M, X476P, X476Q, X476R, X476S, X476T, X476V, X476W, X476Y, X487A, X487C, X487D, X487E, X487F, X487G, X487H, X487I, X487L, X487M, X487N, X487P, X487Q, X487R, X487S, X487T, X487V, X487W, X487Y, X488A, X488C, X488D, X488F, X488G, X488H, X488I, X488K, X488L, X488M, X488N, X488P, X488Q, X488R, X488S, X488T, X488V, X488W, X488Y, X489A, X489C, X489D, X489E, X489F, X489G, X489H, X489I, X489L, X489M, X489N, X489P, X489Q, X489R, X489S, X489T, X489V, X489W, X489Y, X490A, X490C, X490D, X490E, X490F, X490G, X490H, X490I, X490K, X490M, X490N, X490P, X490Q, X490R, X490S, X490T, X490V, X490W, X490Y, X491A, X491C, X491D, X491E, X491F, X491H, X491I, X491K, X491L, X491M, X491N, X491P, X491Q, X491R, X491S, X491T, X491V, X491W, X491Y, X492A, X492C, X492D, X492E, X492F, X492H, X492I, X492K, X492L, X492M, X492N, X492P, X492Q, X492R, X492S, X492T, X492V, X492W, X492Y, X493A, X493C, X493D, X493E, X493F, X493G, X493H, X493I, X493K, X493L, X493M, X493N, X493P, X493Q, X493R, X493T, X493V, X493W, X493Y, X495A, X495C, X495D, X495E, X495G, X495H, X495I, X495K, X495L, X495M, X495N, X495P, X495Q, X495R, X495S, X495T, X495V, X495W, X495Y, X496C, X496D, X496E, X496F, X496G, X496H, X496I, X496K, X496L, X496M, X496N, X496P, X496Q, X496R, X496S, X496T, X496V, X496W, X496Y, X497A, X497C, X497D, X497E, X497F, X497G, X497H, X497I, X497L, X497M, X497N, X497P, X497Q, X497R, X497S, X497T, X497V, X497W, X497Y, X498A, X498C, X498D, X498E, X498F, X498G, X498H, X498I, X498K, X498L, X498M, X498N, X498Q, X498R, X498S, X498T, X498V, X498W, X498Y, X509A, X509C, X509D, X509E, X509F, X509G, X509H, X509I, X509K, X509L, X509M, X509N, X509P, X509R, X509S, X509T, X509V, X509W, X509Y, X514A, X514C, X514D, X514E, X514F, X514G, X514H, X514I, X514K, X514L, X514M, X514N, X514P, X514Q, X514R, X514S, X514T, X514V, X514W, X521A, X521C, X521D, X521E, X521F, X521G, X521H, X521I, X521K, X521L, X521M, X521N, X521P, X521Q, X521R, X521S, X521V, X521W, X521Y, X539A, X539C, X539D, X539E, X539F, X539G, X539H, X539K, X539L, X539M, X539N, X539P, X539Q, X539R, X539S, X539T, X539V, X539W, X539Y, X540A, X540C, X540D, X540E, X540F, X540G, X540H, X540I, X540K, X540M, X540N, X540P, X540Q, X540R, X540S, X540T, X540V, X540W, X540Y, X544A, X544C, X544D, X544E, X544F, X544G, X544H, X544I, X544K, X544L, X544M, X544N, X544P, X544Q, X544S, X544T, X544V, X544W, and X544Y; wherein X represents any amino acid; and wherein each amino acid residue position is numbered by correspondence with an amino acid residue position in the P. alba isoprene synthase sequence as shown in FIG. 20.
In some embodiments, the isoprene synthase variant is not a wild-type isoprene synthase sequence. In some embodiments, the isoprene synthase variant is not a sequence described in PCT/US2009/041581 (WO 2009/132220) or PCT/US2010/032134 (WO 2010/124146).
In some embodiments, the isoprene synthase variant does not comprise an amino acid residue substitution disclosed in PCT/US2009/041581 (WO 2009/132220).
In some embodiments, the isoprene synthase variant does not comprise an amino acid residue substitution in Table D.







TABLE D





Amino Acid Substitutions















For the following sequences, the SEQ ID NOS and residue numbering are as used in


PCT/US2010/032134 (WO 2010/124146):


Met 1, Arg 2, Arg 3, Ser 4, Ala 5, Asn 6, Tyr 7, Glu 8, Pro 9, Asn 10, Ser 11, Trp 12, Asp


13, Tyr 14, Asp 15, Tyr 16, Leu 17, Leu 18, Ser 19, Ser 20, Asp 21, Thr 22, Asp 23, Glu


24, Ser 25, Ile 26, Glu 27, Val 28, Leu 438, Ala 439, Ser 440, Ala 441, Ser 442, Ala 443,


Glu 444, Ile 445, Ala 446, Arg 447, Gly 448, Glu 449, Thr 450, Ala 451, Asn 452, Ser


453, Tyr 512, His 513, Asn 514, Gly 515, Asp 516, Ala 517, His 518, Thr 519, Ser 520,


Pro 521, Asp 522, Glu 523, Leu 524, Thr 525, Arg 526, D293, Y385, S392, D437, S261,


W264, F285, T289, S393, S394, F432, Y512, 40, A441, S442, A443, E444, I445, A446,


R447, G448, E449, T450, A451, N452, S453, Y512, H513, N514, G515, D516, A517,


H518, T519, S520, P521, D522, E523, L524, L17, L18, S19, S20, S239, R243, F253,


A254, R255, D256, R257, I259, E260, D293, Y295, D296, V297, Y298, G299, T300,


E303, Y325, L374, Y375, V529, L530, T534, D293, Y295, V297, E370, A371, W373,


L374, S378, T379, P380, F382, Y385, F386, R433, L434 C435, N436, D437, V454,


S455, C456, Y457, M458, T469, V472, I476, Y512, E187, L188, R255, R257, F270,


E271, Q273, Y274, F285, V288, A439, S440, S442, S508, H509, C510, T511, Y512,


R528, V529, L530, S531, or V532 of SEQ ID NO: 11 as listed in PCT/US2010/032134


(WO 2010/124146) wherein amino acid residue numbering is described at page 84,


paragraph [0295] of PCT/US2010/032134 (WO 2010/124146).


A20, N21, Y22, Q23, P24, N25, L26, E30, F31, Q33, L35, E36, N37, L39, K40, V41,


K43, L44, C57, R61, V62, D63, Q65, K87, E94, N95, L99, D100, N105, K137, E138,


G143, E144, N182, L184, K185, G187, N189, T190, P225, H226, K247, T257, E258,


M259, D266, R271, W278, C291, F299, V302, Y309, D310, N334, D353, S357, I358,


E361, L377, F381, E384, N389, I392, I393, K398, Y399, E401, N402, A403, S406,


S407, S408, G409, A411, L413, C421, Q423, Q424, E425, D426, H430, L432, R433,


S434, D437, R443, C446, F449, A456, T457, S458, A459, A460, E461, L462, E463,


R464, G465, E466, T467, T468, N469, H476, N478, D479, Q485, D508, P513, A515,


M523, S527, Y531, Q532, Y533, L537, G538, R539, Y542, A543, or P557 of SEQ ID


NO: 42 as listed in PCT/US2010/032134 (WO 2010/124146).


L70R of SEQ ID NO: 42 as listed in PCT/US2010/032134 (WO 2010/124146).


A22, N23, Y24, E25, P26, N27, K272, R274, W281, F302, V305, Y312, D313, L380,


F384, E387, Y402, N404, A406, S409, S410, S411, G412, L414, Q415, L416, F449,


N453, L454, A455, S456, A457, S4548, A459, E460, I461, A462, R463, G464, E465,


T466, N469, C497, L521, S525, S537, or E540 of SEQ ID NO: 43 as listed in


PCT/US2010/032134 (WO 2010/124146).


I28, V30, L130, G153, V299, L303, L469, L494, R198, I229, L260, D311, D323, A443,


A453, N454, H515, A519, E525, F388, N438, E451, D345, R528, T536, D304, E314,


D311, D323, A443, A453, or N545 of SEQ ID NO: 45 as listed in PCT/US2010/032134


(WO 2010/124146).


T536F, T536Y, T536V, T536I, T536M, T536H, T536C, T536L, T536K, T536A, T536S,


T536G, A443S, A443G, A443R, A443Q, A453L, A453N, A453I, A453V, H515M,


H515Q, A519H, A519S, A519G, A519W, A519T, D311M, D311F, D311L, D311G,


D311I, D311A, D311T, D311R, D311V, D311E, D323M, D323W, D323Y, D323F,


D323I, D323S, D323V, D323A, D323G, D323Q, I229V, I229L, I229C, I229T, I229P,


I229N, L260N, L260M, L260I, I28W, I28T, I28R, I28Y, V30K, L130W, L130K, L130S,


L130Y, L130R, L130V, L130I, L130E, L130D, G153K, G153H, G153L, G153W,


L303I, L469A, L469Q, L494P, L494C, L494I, L494V, L494S, L494G, L494D, I28W,


I28T, I28R, I28Y, V30K, L130W, L130K, L130S, L130Y, L130R, L130V, L130I,


L130E, L130D, G153K, G153H, G153L, G153W, L303I, L469A, L469Q, L494P,


L494C, L494I, L494V, L494S, L494G, G491S, L494D, A453N, G491S, L494P, T536C,


A452N/G491S, A452N/L494P, A452N/T536C, A452N/G491S/L494P, A452N/G491S/


T536C, A453N/L494P/T536C, A453N/G491S/L494P/T536C, G491S/L494P,


G491S/T536C, G491S/L494P/T536C, or L494P/T536C of SEQ ID NO: 45 as listed in


PCT/US2010/032134 (WO 2010/124146).


 


For the following sequences, the SEQ ID NOS and residue numbering are as used in


PCT/US2009/041581 (WO 2009/132220):


An amino acid substitution corresponding to a substitution at one of the following


residues: L26, E30, F31, Q33, L35, E36, N37, L39, K40, V41, K43, L44, R61, V62, D63,


Q65, K87, E94, N95, L99, D100, N105, K137, E138, G143, E144, N182, L184, K185,


G187, N189, T190, P225, H226, K247, T257, E258, M259, D266, N334, D353, S357,


I358I, E361, N389, I392, I393, K398, E401, C421, Q423, Q424, E425, D426, H430,


L432, R433, S434, D437, R443, L462, E463, H476, N478, D479, Q485, D508, P513,


A515, Q532, Y533, L537, G538, R539, Y542, A543, P557, P24, N25, Y309, D310,


L377, F381, E384, Y399, N402, A403, S406, S407, G409, A411, L413, F449, A456,


T457, S458, A459, A460, E461, L462, E463, R464, G465, E466, T467, T468, N469,


M523, S527, Y531, A20, N21, Y22, Q23, R271, W278, F299, V302, S408, Arg 269, Asp


306, Asp 310, Glu 384, Arg 450, Asn 453, Phe 381, Tyr 399, Ala 403, Ser 406, Asn 469,


Tyr 309, Asp 310, Leu 377, Glu 384, Asn 402, Ser 407, Residues 20-25, Ala 456, Thr


457, Ser 458, Ala 459, Ala 460, Glu 461, Leu 462, Glu 463, Arg 464, Gly 465, Glu 466,


Thr 467, Thr 468, Arg 271, Trp 278, Phe 299, Val 302, Ser 408, Phe 449, Ser 458, Tyr


531, Gly 409, Ala 411, Leu 413, Met 523, or Ser 527 of SEQ ID NO: 2 as listed in


PCT/US2009/041581 (WO 2009/132220)


 


An amino acid substitution corresponding to one of the following substitutions: V10M,


F12S, T15A, E18G, V58I, V58F, L70Q, L70V, L70T, T71P, V79L, E89D, G94A,


S119F, F120L, G127R, E175V, T212I, S257A, R262G, A266G, F280L, N297K, F305L,


L319M, E323K, A328T, D342E, A359T, K366N, E368D, L374M, S396T, V418S,


K438N, H440R, T442I, T442A, I449V, A469S, K500R, K505Q, G507S, S509N, F511Y,


N532K, L70R, L70W, G94E, F305L, V418M, V418T, T442V of SEQ ID NO: 120 as


listed in PCT/US2009/041581 (WO 2009/132220)


 


A combination of amino acid substitutions corresponding to one of the following


combinations of substitutions: G127R/F511Y, L70Q/G94A/R262G/F305L, F12S/


T15A/E18G/N297K, S396T/T442I, V10M/E323K, F120L/A266G, K438N/K500R,


V79L/S509N, E175V/S257A/E368D/A469S, T71P/L374M, F280L/H440R,


E89D/H440R, V58F/A328T/N532K, S119F/D342E/I449V, K366N/G507S of SEQ ID


NO: 120 as listed in PCT/US2009/041581 (WO 2009/132220)


 


An amino acid substitution corresponding to a substitution at one of the following


residues: F303, V306, F385, S412, Q416, F450, V418, or T442 of SEQ ID NO: 120 as


listed in PCT/US2009/041581 (WO 2009/132220)


 


An amino acid substitution corresponding to a substitution at one of the following


residues: Lys 272, Asp 309, Asp 313, Glu 387, Arg 450, Asn 453, Phe 384, Tyr 402, Ala


406, Ser 409, Ala 460, Asn 469, Phe 384, Tyr 402, Ala 406, Ser 409, Asn 469, Tyr 312,


Asp 313, Leu 380, Glu 387, Asn 404, Ser 410, Arg 274, Trp 281, Phe 302, Val 305, Ser


411, Gln 415, Phe 449, Gly 412, Leu 414, Leu 416, Leu 521, Ser 525, Ala 22, Asn 23,


Tyr 24, Glu 25, Pro 26, Asn 27, or Cys 497 of SEQ ID NO: 7 as listed in


PCT/US2009/041581 (WO 2009/132220)


 


An amino acid substitution, or a combination of amino acid substitutions corresponding


to one of the following: K272R, K272R/N453D, K272R/N453D/C497W, or 272R/497W


of SEQ ID NO: 7 as listed in PCT/US2009/041581 (WO 2009/132220)


 


An amino acid substitution corresponding to a substitution at one of the following


residues: L454, A455, S456, A457, S458, A459, E460, I461, A462, R463, G464, E465,


or T466 of SEQ ID NO: 9 as listed in PCT/US2009/041581 (WO 2009/132220)


 


An amino acid substitution corresponding to a substitution at one of the following


residues: Leu 454, Ala 455, Ser 456, Ala 457, Ser 458, Ala 459, Glu 460, Ile 461, Ala


462, Arg 463, Gly 464, Glu 465, or Thr 466 of SEQ ID NO: 124 as listed in


PCT/US2009/041581 (WO 2009/132220)


 


An amino acid substitution corresponding to a substitution at one of the following


residues: Met 1, Arg 2, Arg 3, Ser 4, Ala 5, Asn 6, Tyr 7, Glu 8, Pro 9, Asn 10, Ser 11,,


Trp 12, Asp 13, Tyr 14, Asp 15, Tyr 16 Leu 17, Leu 18, Ser 19, Ser 20, Asp 21, Thr 22,


Asp 23, Glu 24, Ser 25, Ile 26, Glu 27, Val 28, Leu 438, Ala 439, Ser 440, Ala 441, Ser


442, Ala 443, Glu 444, Ile 445, Ala 446, Arg 447, Gly 448, Glu 449, Thr 450, Ala 451,


Asn 452, Ser 453, Tyr 512, His 513, Asn 514, Gly 515, Asp 516, Ala 517, His 518, Thr


519, Ser 520, Pro 521, Asp 522, Glu 523, Leu 524, Thr 525, Arg 526, L17, L18, S19,


S20, S239, R243, F253, A254, R255, D256, R257, I259, E260, D293, Y295, D296,


V297, Y298, G299, T300, E303, Y325, L374, Y375, V529, L530, T534, D293, Y295,


V297, E370, A371, W373, L374, S378, T379, P380, F382, Y385, F386, R433, L434


C435, N436, D437, V454, S455, C456, Y457, M458, T469, V472, I476, Y512, E187,


L188, R255, R257, F270, E271, Q273, Y274, F285, V288, A439, S440, S442, S508,


H509, C510, T511, Y512, R528, V529, L530, S531, V532, Asp 293, Tyr 385, Ser 392,


Asp 437, Ser 440, Ala 441, Ser 442, Ala 443, Glu 444, Ile 445, Ala 446 Arg 447, Gly


448, Glu 449, Thr 450, Ala 451, Asn 452, Ser 453, Tyr 512, His 513, Asn 514, Gly 515,


Asp 516, Ala 517, His 518, Thr 519, Ser 520, Pro 521, Asp 522, Glu 523, Leu 524, Ser


261, Trp 264, Phe 285, Thr 289, Ser 393, Ser 394, Phe 432, or Tyr 512 of SEQ ID NO:


164 as listed in PCT/US2009/041581 (WO 2009/132220) wherein the amino acid residue


numbering is described at page 127, paragraph [0467], of PCT/US2009/041581 (WO


2009/132220)









In some embodiments, the isoprene synthase variant does not comprise a sequence in Table E. In some embodiments, the sequences shown in Tables E and F can have one or more substitution selected from those shown in those Tables.







TABLE E





Isoprene Synthase Sequences















For the following sequences, the SEQ ID NOS and residue numbering are as used in


PCT/US2010/032134 (WO 2010/124146):


SEQ ID NO: 5 (P. tremuloides IspS in P. tremuloides pET24a)


SEQ ID NO: 9 (P. alba isoprene synthase variant in pDu30)


SEQ ID NO: 11 (P. tremuloides isoprene synthase variant P. trem. TRC-pET200 in pDu3)


SEQ ID NO: 13 (P. trichocharpa isoprene synthase)


SEQ ID NO: 16 (P. alba isoprene synthase)


SEQ ID NO: 20 (P. alba FL (+) TEV in MD09-161)


SEQ ID NO: 42 (kudzu isoprene synthase) - L70R variant


SEQ ID NO: 43 (Populus alba × tremuloides)


SEQ ID NO: 45 (P. alba isoprene synthase) - having any one of the following mutations


or combinations of substitutions: T536F, T536Y, T536V, T536I, T536M, T536H,


T536C, T536L, T536K, T536A, T536S, T536G, A443S, A443G, A443R, A443Q,


A453L, A453N, A453I, A453V, H515M, H515Q, A519H, A519S, A519G, A519W,


A519T, D311M, D311F, D311L, D311G, D311I, D311A, D311T, D311R, D311V,


D311E, D323M, D323W, D323Y, D323F, D323I, D323S, D323V, D323A, D323G,


D323Q, I229V, I229L, I229C, I229T, I229P, I229N, L260N, L260M, L260I, I28W,


I28T, I28R, I28Y, V30K, L130W, L130K, L130S, L130Y, L130R, L130V, L130I,


L130E, L130D, G153K, G153H, G153L, G153W, L303I, L469A, L469Q, L494P,


L494C, L494I, L494V, L494S, L494G, L494D, I28W, I28T, I28R, I28Y, V30K, L130W,


L130K, L130S, L130Y, L130R, L130V, L130I, L130E, L130D, G153K, G153H, G153L,


G153W, L303I, L469A, L469Q, L494P, L494C, L494I, L494V, L494S, L494G, G491S,


L494D, A453N, G491S, L494P, T536C, A452N/G491S, A452N/L494P, A452N/T536C,


A452N/G491S/L494P, A452N/G491S/T536C, A453N/L494P/T536C,


A453N/G491S/L494P/T536C, G491S/L494P, G491S/T536C, G491S/L494P/T536C,


L494P/T536C


 


For the following sequences, the SEQ ID NOS and residue numbering are as used in


PCT/US2009/041581 (WO 2009/132220):


SEQ ID NO: 2


SEQ ID NO: 2 A20G variant


SEQ ID NO: 2 S408D variant


SEQ ID NO: 2 C57S/C291S/C421S/C446S variant


SEQ ID NO: 150 of Kudzu TRC (MEA) in pDu50.


SEQ ID NO: 152 of KudzuTRC (−4) in pDu50-4.


SEQ ID NO: 120


SEQ ID NO: 120 - having any one of the following mutations: V10M, F12S, T15A,


E18G, V58I, V58F, L70Q, L70V, L70T, T71P, V79L, E89D, G94A, S119F, F120L,


G127R, E175V, T212I, S257A, R262G, A266G, F280L, N297K, F305L, L319M,


E323K, A328T, D342E, A359T, K366N, E368D, L374M, S396T, V418S, K438N,


H440R, T442I, T442A, I449V, A469S, K500R, K505Q, G507S, S509N, F511Y, N532K,


L70R, L70W, G94E, F305L, V418M, V418T, T442V


SEQ ID NO: 120 - having any one of the following combinations of mutations:


G127R/F511Y, L70Q/G94A/R262G/F305L, F12S/T15A/E18G/N297K, S396T/T442I,


V10M/E323K, F120L/A266G, K438N/K500R, V79L/S509N,


E175V/S257A/E368D/A469S, T71P/L374M, F280L/H440R, E89D/H440R,


V58F/A328T/N532K, S119F/D342E/I449V, K366N/G507S


SEQ ID NO: 122 (MEA variant)


SEQ ID NO: 124 (MSV variant)


SEQ ID NO: 126 (MVS variant)


SEQ ID NO: 128 (MTE variant)


SEQ ID NO: 130 (MNV variant)


TRC (−3) variant (SEQ ID NO: 136)


TRC (−4) variant (SEQ ID NO: 138)


TRC (−5) variant (SEQ ID NO: 140)


TRC (−6) variant (SEQ ID NO: 142)


TRC (−7) variant (SEQ ID NO: 144)


SEQ ID NO: 7


SEQ ID NO: 7 - having any one or more of the following substitutions: K272R, N453D,


C497W


SEQ ID NO: 7 - having any one of the following combinations of substitutions:


K272R/N453D; K272R/N453D/C497W, 272R/497W


(SEQ ID NO: 132) of P. alba MEA(+)TEV in MD09-163.


(SEQ ID NO: 134) of P. alba FL(+)TEV in MD09-161.


(SEQ ID NO: 162) of IspS variant P. alba TRC-pET200 in pDu30.


Sequences for MD08-99, MD08-100, MD09-167, MD08-104, MD08-102


MET variant of P. tremuloides isoprene synthase (SEQ ID NO: 146).


(SEQ ID NO: 158) of P. tremuloides IspS in P. trichocharpa pET24a.


(SEQ ID NO: 164) of IspS variant P. trem TRC-pET200 in pDu31.


MET variant of P. trichocharpa isoprene synthase (SEQ ID NO: 148).


(SEQ ID NO: 160) of P. trichocharpa IspS in P. trichocharpa pET24a.


(SEQ ID NO: 166) of IspS variant P. trich TRC-pET200 in pDu32.









In some embodiments, the isoprene synthase variant does not comprise a sequence in Table F.







TABLE F







For the following sequences, the SEQ ID NOS and residue numbering are as used in


PCT/US2010/032134 (WO 2010/124146)


SEQ ID NO: 11 (P. tremuloides isoprene synthase variant P. trem. TRC-pET200 in pDu3)


having a substitution at one of the following residues: Met 1, Arg 2, Arg 3, Ser 4, Ala 5,


Asn 6, Tyr 7, Glu 8, Pro 9, Asn 10, Ser 11, Trp 12, Asp 13, Tyr 14, Asp 15, Tyr 16, Leu


17, Leu 18, Ser 19, Ser 20, Asp 21, Thr 22, Asp 23, Glu 24, Ser 25, Ile 26, Glu 27, Val


28, Leu 438, Ala 439, Ser 440, Ala 441, Ser 442, Ala 443, Glu 444, Ile 445, Ala 446, Arg


447, Gly 448, Glu 449, Thr 450, Ala 451, Asn 452, Ser 453, Tyr 512, His 513, Asn 514,


Gly 515, Asp 516, Ala 517, His 518, Thr 519, Ser 520, Pro 521, Asp 522, Glu 523, Leu


524, Thr 525, Arg 526, D293, Y385, S392, D437, S261, W264, F285, T289, S393, S394,


F432, Y512, 40, A441, S442, A443, E444, I445, A446, R447, G448, E449, T450, A451,


N452, S453, Y512, H513, N514, G515, D516, A517, H518, T519, S520, P521, D522,


E523, L524, L17, L18, S19, S20, S239, R243, F253, A254, R255, D256, R257, I259,


E260, D293, Y295, D296, V297, Y298, G299, T300, E303, Y325, L374, Y375, V529,


L530, T534, D293, Y295, V297, E370, A371, W373, L374, S378, T379, P380, F382,


Y385, F386, R433, L434 C435, N436, D437, V454, S455, C456, Y457, M458, T469,


V472, I476, Y512, E187, L188, R255, R257, F270, E271, Q273, Y274, F285, V288,


A439, S440, S442, S508, H509, C510, T511, Y512, R528, V529, L530, S531, or V532,


wherein amino acid residue numbering is described at page 84, paragraph [0295] of


PCT/US2010/032134 (WO 2010/124146).


 


SEQ ID NO: 42 (kudzu isoprene synthase) having a substitution at one of the following


residues: A20, N21, Y22, Q23, P24, N25, L26, E30, F31, Q33, L35, E36, N37, L39, K40,


V41, K43, L44, C57, R61, V62, D63, Q65, K87, E94, N95, L99, D100, N105, K137,


E138, G143, E144, N182, L184, K185, G187, N189, T190, P225, H226, K247, T257,


E258, M259, D266, R271, W278, C291, F299, V302, Y309, D310, N334, D353, S357,


I3581, E361, L377, F381, E384, N389, I392, I393, K398, Y399, E401, N402, A403,


S406, S407, S408, G409, A411, L413, C421, Q423, Q424, E425, D426, H430, L432,


R433, S434, D437, R443, C446, F449, A456, T457, S458, A459, A460, E461, L462,


E463, R464, G465, E466, T467, T468, N469, H476, N478, D479, Q485, D508, P513,


A515, M523, S527, Y531, Q532, Y533, L537, G538, R539, Y542, A543, and P557


 


SEQ ID NO: 43 (Populus alba x tremuloides) having a substitution at one of the


following residues: A22, N23, Y24, E25, P26, N27, K272, R274, W281, F302, V305,


Y312, D313, L380, F384, E387, Y402, N404, A406, S409, S410, S411, G412, L414,


Q415, L416, F449, N453, L454, A455, S456, A457, S4548, A459, E460, I461, A462,


R463, G464, E465, T466, N469, C497, L521, S525, S537, or E540


 


SEQ ID NO: 45 (P. alba isoprene synthase) having a substitution at one of the following


residues: I28, V30, L130, G153, V299, L303, L469, L494, R198, I229, L260, D311,


D323, A443, A453, N454, H515, A519, E525, F388, N438, E451, D345, R528, T536,


D304, E314, D311, D323, A443, A453, N545


 


For the following sequences, the SEQ ID NOS and residue numbering are as used in


PCT/US2009/041581 (WO 2009/132220):


SEQ ID NO: 2 having a substitution at one of the following residues: L26, E30, F31,


Q33, L35, E36, N37, L39, K40, V41, K43, L44, R61, V62, D63, Q65, K87, E94, N95,


L99, D100, N105, K137, E138, G143, E144, N182, L184, K185, G187, N189, T190,


P225, H226, K247, T257, E258, M259, D266, N334, D353, S357, I3581, E361, N389,


I392, I393, K398, E401, C421, Q423, Q424, E425, D426, H430, L432, R433, S434,


D437, R443, L462, E463, H476, N478, D479, Q485, D508, P513, A515, Q532, Y533,


L537, G538, R539, Y542, A543, P557, P24, N25, Y309, D310, L377, F381, E384, Y399,


N402, A403, S406, S407, G409, A411, L413, F449, A456, T457, S458, A459, A460,


E461, L462, E463, R464, G465, E466, T467, T468, N469, M523, S527, Y531, A20,


N21, Y22, Q23, R271, W278, F299, V302, S408, Arg 269, Asp 306, Asp 310, Glu 384,


Arg 450, Asn 453, Phe 381, Tyr 399, Ala 403, Ser 406, Asn 469, Tyr 309, Asp 310, Leu


377, Glu 384, Asn 402, Ser 407, Residues 20-25, Ala 456, Thr 457, Ser 458, Ala 459, Ala


460, Glu 461, Leu 462, Glu 463, Arg 464, Gly 465, Glu 466, Thr 467, Thr 468, Arg 271,


Trp 278, Phe 299, Val 302, Ser 408, Phe 449, Ser 458, Tyr 531, Gly 409, Ala 411, Leu


413, Met 523, or Ser 527


 


SEQ ID NO: 120 having a substitution at one of the following residues: F303, V306,


F385, S412, Q416, F450, V418, T442


 


SEQ ID NO: 7 - having a substitution at one of the following residues: Lys 272, Asp 309,


Asp 313, Glu 387, Arg 450, Asn 453, Phe 384, Tyr 402, Ala 406, Ser 409, Ala 460, Asn


469, Phe 384, Tyr 402, Ala 406, Ser 409, Asn 469, Tyr 312, Asp 313, Leu 380, Glu 387,


Asn 404, Ser 410, Arg 274, Trp 281, Phe 302, Val 305, Ser 411, Gln 415, Phe 449, Gly


412, Leu 414, Leu 416, Leu 521, Ser 525, Ala 22, Asn 23, Tyr 24, Glu 25, Pro 26, Asn


27, Cys 497


 


SEQ ID NO: 9 - having a substitution at one of the following residues 454-466:


LASASAEIARGET


 


SEQ ID NO: 124 (MSV variant) - having a substitution at one of the following residues:


Leu 454, Ala 455, Ser 456, Ala 457, Ser 458, Ala 459, Glu 460, Ile 461, Ala 462, Arg


463, Gly 464, Glu 465, Thr 466,


 


SEQ ID NO: 164 having a substitution at one of the following residues: Met 1, Arg 2,


Arg 3, Ser 4, Ala 5, Asn 6, Tyr 7, Glu 8, Pro 9, Asn 10, Ser 11, Trp 12, Asp 13, Tyr 14,


Asp 15, Tyr 16 Leu 17, Leu 18, Ser 19, Ser 20, Asp 21, Thr 22, Asp 23, Glu 24, Ser 25,


Ile 26, Glu 27, Val 28, Leu 438, Ala 439, Ser 440, Ala 441, Ser 442, Ala 443, Glu 444,


Ile 445, Ala 446, Arg 447, Gly 448, Glu 449, Thr 450, Ala 451, Asn 452, Ser 453, Tyr


512, His 513, Asn 514, Gly 515, Asp 516, Ala 517, His 518, Thr 519, Ser 520, Pro 521,


Asp 522, Glu 523, Leu 524, Thr 525, Arg 526, L17, L18, S19, S20, S239, R243, F253,


A254, R255, D256, R257, I259, E260, D293, Y295, D296, V297, Y298, G299, T300,


E303, Y325, L374, Y375, V529, L530, T534, D293, Y295, V297, E370, A371, W373,


L374, S378, T379, P380, F382, Y385, F386, R433, L434 C435, N436, D437, V454,


S455, C456, Y457, M458, T469, V472, I476, Y512, E187, L188, R255, R257, F270,


E271, Q273, Y274, F285, V288, A439, S440, S442, S508, H509, C510, T511, Y512,


R528, V529, L530, S531, V532, Asp 293, Tyr 385, Ser 392, Asp 437, Ser 440, Ala 441,


Ser 442, Ala 443, Glu 444, Ile 445, Ala 446 Arg 447, Gly 448, Glu 449, Thr 450, Ala


451, Asn 452, Ser 453, Tyr 512, His 513, Asn 514, Gly 515, Asp 516, Ala 517, His


518, Thr 519, Ser 520, Pro 521, Asp 522, Glu 523, Leu 524, Ser 261, Trp 264, Phe 285,


Thr 289, Ser 393, Ser 394, Phe 432, Tyr 512 wherein the amino acid residue numbering


is described at page 127, paragraph [0467], of PCT/US2009/041581 (WO 2009/132220)









Residue numbering for the sequences and substitutions in Tables 4-6 are as defined in PCT/US2009/041581 (WO 2009/132220) or PCT/US2010/032134 (WO 2010/124146), as noted. One of skill in the art can determine how residues of Tables 4-6 correspond with those of a reference sequence, such as MEA P. alba (FIG. 20) (SEQ ID NO:1).
N-Terminal Truncation
In some embodiments, the variant comprises an N-terminal region truncation comprising truncation of one or more amino acid residues of the N-terminal region. See, for example, Example 10. Examples of N-terminal truncations are described in PCT/US2009/041581 (WO 2009/132220). In some embodiments, the isoprene synthase variant comprising an N-terminal truncation has an increased specific activity compared to a full length isoprene synthase. Examples of N-terminal truncations include, for example, the residues corresponding to the N-terminal truncated residues of P. alba isoprene synthases: MEA variant, MSV variant, MVS variant, MTE variant, MNV variant, a TRC (−3) variant, a TRC (−4) variant, a TRC (−5) variant, a TRC (−6) variant and a TRC (−7) variant; P. tremuloides isoprene synthase: MET variant; and P. trichocharpa isoprene synthase: MET variant, wherein the sequences are described in PCT/US2009/041581 (WO 2009/132220). In some embodiments, the truncated residues of the variant correspond to the residues truncated in the P. alba isoprene synthase MEA variant (See FIG. 21A-21B).
Isoprene Synthase Variant Properties
In some embodiments, the variant has at least one improved property over a reference sequence. In some embodiments, the reference sequence is the parent sequence. In some embodiments, the reference sequence is a wild-type isoprene synthase. In some embodiments, the reference sequence is MEA P. alba (SEQ ID NO:1), also shown in FIG. 20.
Properties of interest include, but are not limited to: increased intracellular activity, specific productivity, yield, and cellular performance index. In some embodiments, specific productivity increase at least about 2, 3, 4, 5, 6 7, 8, 9, 10 times or more. In one embodiment, specific productivity is about 20 mg/L/OD/hr. In other embodiments, yield increase at least about 2, 3, 4, 5 times or more. In other embodiments, cell performance index increase at least about 2, 3, 4, 5 times or more. In other embodiments, intracellular activity increase at least about 2, 3, 4, 5, 6, 7, 8, 9, 10 times or more.
Without being bound by theory, these properties can be achieved by one or a combination of any of the following properties of IspS: increased cellular viability, increased kcat, decreased Km, increased specific activity, increased solubility, decreased insolubility, improved ribosome binding, increased translation initiation rate, increased translation elongation rate, increased transcription initiation rate, increased transcription elongation rate, decreased secondary structure of DNA, decreased secondary structure of RNA, increased secondary structure of DNA, increased secondary structure of RNA, increased folding rates, increased affinity for intracellular chaperones, increased stability, decreased protein turnover, decreased exposure to intracellular protease, decreased affinity for intracellular protease, decreased localization to the periplasm, improved localization to the cytoplasm, decreased inclusion body formation, decreased membrane localization, increased expression due to a more favorable codon, increased DNA stability, increased RNA stability, and decreased RNA degradation. In brief, any mutation that has a positive effect on the properties of nucleic acid sequences (DNA and RNA) encoding or expressing the IspS variant, or the biochemical properties of the IspS enzyme itself, could allow for greater activity within the cell. Other properties of interest include pH optima, temperature stability (e.g., Tm value), as well as sensitivity to potential inhibitors including substrate or product inhibition. Oxidative and proteolytic stability are also of interest. Furthermore, activation or inhibition due to metal ion effects and ionic strength is of interest.
In one embodiment, specific activity values can be calculated for every variant in the entire set of SELs by dividing the molar amount of isoprene produced in a given amount of time by the specific amount of protein in each sample. Performance index (PI) can be calculated by dividing the specific activity of any given variant by the average of several WT specific activity measurements from the same microtiter plate. For example, a variant that displayed a PI value of 1.5 for specific activity would be 50% improved over WT. PIs for protein concentration and isoprene produced can also be calculated in the same fashion. These measurements were used for detailed data analysis as shown in the Examples.
Growth index or performance index of a host cell comprising a nucleic acid encoding an isoprene synthase variant may also be used to indicate whether a particular variant has a property of interest. Growth index and performance index may be determined according to methods known to one of skill in the art and/or as taught herein. Growth and performance index may be determined for a particular variant by comparison with a reference sequence. In some embodiments, the reference sequence is the parent sequence of the variant. In some embodiments, the reference sequence is a wild type sequence. In some embodiments, the reference sequence is MEA P. alba. In some embodiments, growth index is determined according to the method in Example 2. In some embodiments, growth index is determined according to the method in Example 4. In various embodiments, the growth index of the variant is at least about 0.8, at least about 0.9, at least about 1.0, at least about 1.1, at least about 1.2, at least about 1.3, as compared with the reference sequence. In various embodiments, the performance index of the variant is at least about 0.7, at least about 0.8, at least about 0.9, at least about 1.0, at least about 1.1, at least about 1.2, as compared with the reference sequence.
Methods for determining the properties of interest are known tone of skill in the art. Certain methods are further described herein in the Examples. Variants can be assessed based on the desired outcome or property to be improved. For example, a variant isoprene synthase engineered for increased specific activity can be tested for the conversion of DMAPP to isoprene in vitro with purified or partially purified variant isoprene synthase or in vivo in the context of a host organism such as E. coli. In some cases, the E. coli may also express the DXP pathway, the MVA pathway, or both. Improved activity is assessed in comparison with other isoprene synthases; for example, a wild type isoprene synthase, a parent isoprene synthase, or other reference polypeptide. It is contemplated that enzymes having various degrees of e.g. stability, solubility, activity, and/or expression level in one or more of test conditions will find use in the present invention for the production of isoprene in a diversity of hosts. High throughput methods may provide an investigation of these properties in an economical manner.
There is a strong correlation between increased intracellular DMAPP levels and growth inhibition of E. coli, which can be alleviated by the expression of P. alba Isoprene Synthase (IspS). Without being bound by theory, increased levels of IspS activity should therefore allow for better growth due to more rapid conversion of DMAPP to isoprene. By monitoring the growth rates of E. coli expressing variants of IspS under these conditions, the inventors have identified mutant IspS enzymes that display increased ability to convert DMAPP to isoprene within the cell.
The invention also contemplates methods for screening for isoprene synthase variants, comprising: (a) contacting a host cell with a medium comprising about 10 μM to about 70 μM IPTG, and about 5 mM to about 20 mM mevalonic acid (MVA), wherein the host cell comprises a nucleic acid encoding an isoprene synthase variant in operable combination with a promoter; and (b) measuring the growth rate of the host cell. The variant growth rate may be compared to that of a reference isoprene synthase (e.g. a parent isoprene synthase, a wild-type isoprene synthase, or MEA P. alba isoprene synthase. The methods may be used to screen for variants having a particular property of interest, for example, one or more of the properties described herein. In some embodiments, an increased growth rate indicates an isoprene synthase variant with an increased ability to convert DMAPP to isoprene within the host cell synthase. Growth rates may be analyzed, for example, according to methods known in the art, or as exemplified in the Examples below. In some embodiments, the method further comprises determining a growth index for the variant. In some embodiments, the method further comprises determining a performance index for the variant. Growth rate of the cells in exponential phase and/or final density of the cells may be taken into consideration as factors when selecting variants. As exemplied below, for the variants shown in the examples, the growth rate of the cells in exponential phase was a consideration. In addition, growth rate and final density was also taken into consideration when selecting for variants described herein.
In some embodiments, the IPTG is present in the medium at a concentration from about 10 μM to about 60 μM. In some embodiments, the IPTG is present in the medium at a concentration from about 10 μM to about 200 μM. In some embodiments, the IPTG is present in the medium at a concentration from about 20 μM to about 60 μM. In some embodiments, IPTG is present in the medium at a concentration from about 20 μM to about 120 μM. In some embodiments, the IPTG is present in the medium at a concentration from about 40 μM to about 60 μM. In some embodiments, the IPTG is present in the medium at a concentration from about 40 μM to about 100 μM. In some embodiments, the IPTG is present in the medium at a concentration of about 40 μM. In some embodiments, the IPTG is present in the medium at a concentration of about 50 μM, 60 μM, 70 μM, 80 μM, 90 μM, 100 μM, 110 μM, 120 μM, 130 μM, 140 μM, 150 μM, 160 μM, 170 μM, 180 μM, 190 μM, or 200 μM.
In some embodiments, the MVA is present in the medium at a concentration of about 5 mM to about 20 mM. In some embodiments, the MVA is present in the medium at a concentration of about 7 mM to about 15 mM. In some embodiments, the MVA is present in the medium at a concentration of about 8 mM to about 12 mM. In some embodiments, the MVA is present in the medium at a concentration of about 10 mM. In some embodiments, the host cell is MD09-170.
In another aspect, one or more improved properties can be seen by analyzing productive positions as further described below and exemplified in the Examples. Productive positions can be described as those positions within a molecule (e.g., enzyme such as isoprene synthase) that are most useful for making combinatorial variants exhibiting an improved characteristic, where the position itself allows for at least one combinable mutation. Combinable mutations can be described as those substitutions in a molecule that can be used to make selected combinatorial variants. Combinable mutations do not significantly decrease expression, specific activity or growth, while at the same time improving at least one desired characteristic of the molecule such as growth or specific activity. Positions in IspS containing all combinable mutations can be determined using performance index (PI) values resulting from the DMAPP assay for specific activity, growth curves of MEA P. alba expressing the indicated IspS variant and protein determination, as exemplified in Example 1 and Example 2. Productive positions can be the positions which have shown a certain degree of tolerance for multiple substitutions, while at the same time meeting a set of criteria for combinability as set forth below.
When evaluating the data set, the most productive positions were determined when the following criteria were applied. For positions containing substitutions where the minimum performance indices (PI) relative to wild type IspS for specific activity and expression are greater than or equal to a PI of 0.9 and where at least one PI relative to wild type IspS for specific activity or growth is greater than or equal to a PI of 1.0, these positions are classified as Group A. For positions containing substitutions where the minimum performance indices (PI) relative to wild type IspS for specific activity and expression are greater than or equal to a PI of 0.8 and where at least one PI relative to wild type IspS for specific activity or growth is greater than or equal to a PI of 1.2, these positions are classified as Group B. For positions containing substitutions where the minimum performance indices (PI) relative to wild type IspS for specific activity and expression are greater than or equal to a PI of 0.5 and where at least one PI relative to wild type IspS for specific activity or growth is greater than or equal to a PI of 1.5, these positions are classified as Group C.
Groups A, B, and C can further contain positions that have differing degrees of tolerance for multiple substitutions. To measure this degree of substitutions tolerated, a Rank can be assigned to each position. The Rank can be assigned according to the percentage of the substitutions within each position that fall within groups A, B, or C. Exemplary combinable positions and substitutions are shown in Table 31.
The criteria to determine the Rank for productive positions are as follows: Positions where less than 15% but greater than 0% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “1.” Positions where less than 30%, but greater than, or equal to 15% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “2.” Positions where less than 50%, but greater than, or equal to 30% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “3.” Positions where greater than, or equal to 50% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “4.”
Substitutions can be further assigned a Suitability Score based on the group(s) the substitution is a member of, and where a higher score represents a substitution more suitable for use in making combinatorial variants. Exemplary suitability scores are represented and described in Table 30. Suitability scores and rank for individual substitutions of IspS that fit the above criteria are exemplified in Table 31.







TABLE 30







Suitability Score for the defined groups.










Substitutions




Occur in
Suitability



Group(s):
Score







A, B and C
+++++



A and B
++++



A or (B and C)
+++



B
++



C
+










Accordingly, in one aspect of the invention, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that are scored +++++ as exemplified in the Examples. These polypeptides can have one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that is indicated in the column labeled +++++ as exemplified in the Examples. Some non-limiting examples are isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises one or more substitution(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X2, X18, X19, X21, X24, X26, X27, X29, X37, X42, X47, X48, X49, X56, X81, X82, X84, X93, X94, X95, X120, X123, X126, X131, X132, X134, X137, X139, X143, X151, X155, X166, X167, X169, X170, X171, X175, X179, X180, X197, X229, X231, X240, X242, X245, X246, X247, X251, X271, X282, X306, X317, X319, X369, X371, X376, X379, X380, X389, X392, X393, X408, X409, X421, X422, X423, X429, X437, X443, X444, X447, X455, X458, X461, X464, X466, X470, X473, X500, X502, X506, X513, X525 and, X531. In some embodiments, these substitutions can be at a residue selected from the group consisting of E2, Y18, L19, S21, T24, E26, S27, E29, K37, V42, N47, N48, E49, L56, D81, R82, V84, T93, K94, T95, S120, K123, N126, E131, N132, K134, I137, A139, L143, L151, N155, S166, H167, K169, E170, L171, K175, E179, L180, Q197, I229, S231, T240, R242, R245, R246, V247, T251, A271, S282, L306, D317, N319, F369, Q371, L376, K379, S380, G389, W392, K393, V408, V409, Q421, K422, Y423, R429, C437, A443, S444, I447, S455, C458, R461, G464, S466, A470, S473, V500, T502, L506, T513, E525 and, V531. In some embodiments, the substitution is selected from the group consisting of E2A or K or P, Y18D or E or K or S, L19Y, S21W, T24L or V, E26C, S27D or N, E29N, K37C or D or P or Q or S, V42M, N47D or S, N48D or G or T, E49L or V, L56E or F or G or I or K or T or V or Y, D81Q, R82N or T or V or Y, V84M, T93C or F or R or S, K94G or P, T95D or F or G or I or N or W, S120C or G or M or Q, K123V, N126E, E131H or K or L or M or T or W or Y, N132I or P, K134A, I137T, A139C or Q, L143C or D or E or H or K or M or Q or T or V or Y, L151A or F, N155A or C or G or H or Q or R or S or W, S166N, H167F or I or N or Q or V, K169A or C or H or N or Q or V, E170L or S or W or Y, L171A or N or Q or T or V or Y, K175C or F or I or Q or R, E179D, L180A or I, Q197C or D or N, I229C, S231A, T240C, R242G, R245C or K or M or Q or T or V, R246N, V247L or M, T251D or E or N or P or Q or S, A271T, S282Y, L306C, D317N, N319M, F369C or D or E or G or S, Q371F, L376I or M, K379G or Q, S380E, G389A or D or E or K or N or Q or S or V, W392Y, K393C or I or T or V, V408T, V409T, Q421H, K422D, Y423N or S, R429E or F or Q, C437M, A443Q, S444D or E, I447T, S455A, C458T, R461A, G464C or M or N or Q or S, S466D, A470I or L, S473I, V500A or C, T502M, L506M, T513C or G or K or N, E525F or R, V531E or H or K or Q or R or S.
In another aspect of the invention, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that are scored ++++ as exemplified in the Examples. These polypeptides can have one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that is indicated in the column labeled ++++ as exemplified in the Examples. Some non-limiting examples are isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises one or more substitution(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X2, X6, X18, X20, X22, X23, X24, X25, X26, X27, X28, X29, X30, X31, X32, X36, X37, X42, X44, X47, X48, X49, X50, X53, X54, X55, X56, X58, X59, X68, X71, X74, X77, X78, X79, X81, X82, X83, X84, X86, X87, X91, X93, X94, X95, X97, X98, X99, X109, X115, X116, X117, X118, X120, X123, X125, X126, X127, X128, X130, X131, X132, X133, X134, X136, X137, X138, X139, X140, X143, X151, X153, X155, X156, X159, X160, X161, X162, X163, X164, X166, X167, X169, X170, X171, X172, X175, X176, X177, X178, X179, X180, X181, X182, X190, X194, X197, X204, X211, X215, X217, X219, X221, X228, X229, X231, X232, X235, X241, X242, X245, X246, X247, X251, X254, X271, X272, X278, X279, X282, X296, X302, X317, X319, X320, X327, X331, X348, X351, X357, X361, X364, X365, X368, X369, X370, X371, X373, X377, X380, X383, X386, X389, X392, X393, X407, X408, X409, X410, X411, X414, X422, X423, X424, X428, X429, X432, X436, X437, X440, X443, X444, X447, X448, X457, X460, X461, X462, X463, X464, X465, X466, X468, X470, X471, X472, X473, X475, X480, X490, X491, X492, X494, X496, X500, X501, X502, X503, X510, X513, X515, X519, X525, X531, X536, X537, X540, X541, X542, and X544.
In some embodiments, these substitutions can be at a residue selected from the group consisting of: E2, S6, Y18, L20, S22, D23, T24, D25, E26, S27, I28, E29, V30, Y31, K32, K36, K37, V42, R44, N47, N48, E49, K50, F53, L54, T55, L56, E58, L59, L68, R71, S74, R77, G78, A79, D81, R82, F83, V84, S86, G87, A91, T93, K94, T95, L97, H98, G99, Q109, S115, Q116, E117, A118, S120, K123, Q125, N126, G127, N128, L130, E131, N132, L133, K134, D136, I137, K138, A139, I140, L143, L151, G153, N155, I156, E159, A160, K161, V162, F163, A164, S166, H167, K169, E170, L171, S172, K175, I176, G177, K178, E179, L180, A181, E182, L190, R194, Q197, S204, K211, N215, V217, L219, L221, M228, I229, S231, V232, R235, S241, R242, R245, R246, V247, T251, H254, A271, F272, D278, C279, S282, I296, T302, D317, N319, A320, Y327, C331, K348, G351, Y357, A361, D364, L365, A368, F369, L370, Q371, A373, Y377, S380, T383, D386, G389, W392, K393, A407, V408, V409, Q410, N411, K414, K422, Y423, H424, S428, R429, H432, L436, C437, L440, A443, S444, I447, A448, S457, M460, R461, T462, K463, G464, I465, S466, E468, A470, T471, E472, S473, M475, E480, L490, G491, G492, L494, A496, V500, E501, T502, A503, S510, T513, H515, A519, E525, V531, T536, E537, L540, P541, F542, and R544.
In other embodiments, the substitution is at a residue selected from the group consisting of: E2C or D or N or T or V, S6N or T, Y18A or Q or R, L20T, S22Q, D23N, T24C, D25T, E26D or H or K or M or R or S or V, S27A or C or G or H or I or L or M or P or Q, 128D or N, E29Q, V30A or D or E or M or R or T, Y31N, K32E, K36A or C or D or E or M or N or P or Q, K37A or E or G or H or M or N or R or T, V42F or I, R44N or Q, N47A or G or H or M or Q or T or W, N48H or I or K, E49A or C, K50A or D or E or F or H or S or Y, F53E or H or N or P or Q or V, L54M, T55C or D or E, L56C or N, E58N, L59H or T, L68I, R71K or M, S74D or E or N or Y, R77L, G78A or D or F or L or M, A79Q or T, D81A or F or G or M or R or S or T or V, R82A or E or H or I or K or M or Q or S, F83W, V84A, S86A or D or M, G87D or P, A91K or W, T93A or D or E or G or L or N or P or Y, K94A or D or E or H or I or L or M or N or R or S or T, T95A or E or P or Q or S or V or Y, L97F, H98A or D or F or G or I or L or M or N or Q, G99E or F or M, Q109E, S115A, Q116A or C or D or E or I or P, E117C or F or L or M or V, A118M, S120H or T or V, K123L or T, Q125E or I or Y, N126A or C or D or M or T or V, G127C, N128C or D or P or Q, L130E, E131A or C or P or Q or S or V, N132C or D or F or H or L or R or W or Y, L133D, K134E or M or Q or S or T or V, D136E, I137E or H or N, K138I or N, A139N, I140M or W, L143S, L151C or H or I, G153C, N155I or T or V or Y, I156D or N or T, E159M, A160I, K161A or C or N or Q, V162S, F163E or Q, A164T, S166A or D or G, H167A or E or G or K or M or R or S or T or W, K169D or I or M or S or T, E170H or K or M or Q or T or V, L171H or K or R or S, S172A or C, K175S, I176M, G177A or C, K178A or F or R or S or T, E179A or C or L or M or N, L180C or Q or T, A181H or Q or S or V, E182S, L190I or M, R194L, Q197S, S204C, K211A or N or Q, N215C or H, V217I, L219C, L221M, M228F or Y, I229V, S231K or Q or T, V232I, R235K, S241A or M or T, R242A or D or E or H or I or M or N or Q or S or T, R245I or L, R246D or K, V247T, T251A or G or K or R, H254D, A271C or V, F272D or G or P or W, D278A or E or N or Q or S or T or V or W, C279A, S282A or Q, I296V, T302H, D317E or Q, N319F, A320C, Y327M, C331P, K348R or Y, G351D or N, Y357M, A361T, D364E or V, L365C or M, A368N, F369M or N or R or T or V, L370G or Q, Q371C or S, A373G, Y377W, S380A or C or D or Q or T or V, T383S, D386E or N, G389H or I, W392I or S or T or V, K393Q, A407G, V408I, V409H or I, Q410C or D or K or L or M or T, N411G, K414E or G or L or N or P, K422A or N or T, Y423Q, H424E or P or Q or V, S428E or Q, R429I or L or T or W or Y, H432E, L436M or Y, C437K or T, L440I, A443R, S444P, I447A or E or M or Q or S, A448E or M or N or P or Q or V, S457N or T, M460Q or R or S, R461D or E or G or Q or S or T, T462Q, K463A or D or E, G464L or R, I465A or C or G or S or T, S466P, E468D, A470M, T471E or H or Q, E472D or S, S473L or V, M475T, E480N, L490A or D or E or F or H or M, G491E or K or S or T or V or Y, G492C, L494D, A496P or T, V500L or M, E501D, T502A or C or R or V, A503I, S510C or V, T513V, H515N, A519S or T, E525A or C or P or Q or S, V531A or M or T, T536A or F or G, E537K or T, L540A or P, P541M, F542P, and R544C.
In another aspect of the invention, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that are scored +++ as exemplified in the Examples. These polypeptides can have one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that is indicated in the column labeled +++ as exemplified in the Examples. Some non-limiting examples are isolated polypeptides with improved isoprene synthase properties wherein the polypeptide comprises one or more substitution(s) at residues corresponding to SEQ ID NO:1 selected from the group consisting of: X2, X3, X13, X17, X18, X19, X20, X23, X25, X26, X27, X28, X29, X30, X31, X32, X33, X34, X36, X37, X40, X41, X42, X43, X44, X45, X46, X47, X48, X49, X50, X51, X53, X54, X55, X56, X57, X59, X60, X62, X71, X73, X74, X75, X77, X78, X79, X81, X82, X83, X84, X85, X86, X87, X88, X89, X91, X92, X93, X94, X95, X97, X98, X99, X100, X101, X102, X103, X107, X109, X111, X113, X114, X115, X116, X117, X118, X119, X120, X121, X123, X124, X125, X127, X128, X129, X130, X131, X133, X134, X135, X136, X137, X138, X139, X140, X143, X146, X151, X152, X153, X155, X156, X158, X160, X161, X162, X163, X166, X167, X169, X170, X171, X172, X175, X176, X177, X178, X179, X180, X181, X182, X183, X185, X187, X193, X194, X196, X197, X204, X210, X211, X212, X215, X216, X217, X218, X219, X220, X222, X223, X224, X226, X228, X229, X231, X232, X235, X240, X241, X242, X246, X251, X253, X260, X268, X270, X271, X272, X275, X276, X278, X282, X307, X314, X315, X317, X320, X321, X323, X328, X329, X331, X332, X333, X343, X345, X346, X350, X351, X352, X356, X357, X360, X361, X363, X364, X366, X367, X368, X369, X370, X371, X378, X379, X380, X383, X386, X389, X390, X392, X393, X402, X405, X408, X409, X410, X413, X414, X418, X422, X423, X424, X425, X426, X428, X429, X431, X432, X437, X444, X447, X448, X457, X460, X461, X462, X463, X464, X466, X467, X468, X469, X471, X472, X475, X484, X489, X490, X491, X492, X493, X494, X497, X500, X501, X502, X503, X504, X506, X509, X510, X511, X513, X515, X517, X519, X522, X528, X529, X531, X534, X535, X536, X537, X539, X540, X542, and X544.
In some embodiments, these substitutions can be at a residue selected from the group consisting of: E2, A3, S13, D17, Y18, L19, L20, D23, D25, E26, S27, I28, E29, V30, Y31, K32, D33, K34, K36, K37, A40, E41, V42, R43, R44, E45, I46, N47, N48, E49, K50, A51, F53, L54, T55, L56, L57, L59, I60, N62, R71, E73, S74, D75, R77, G78, A79, D81, R82, F83, V84, S85, S86, G87, G88, F89, A91, V92, T93, K94, T95, L97, H98, G99, T100, A101, L102, S103, L107, Q109, G111, E113, V114, S115, Q116, E117, A118, F119, S120, G121, K123, D124, Q125, G127, N128, F129, L130, E131, L133, K134, E135, D136, I137, K138, A139, I140, L143, A146, L151, E152, G153, N155, I156, D158, A160, K161, V162, F163, S166, H167, K169, E170, L171, S172, K175, I176, G177, K178, E179, L180, A181, E182, Q183, N185, A187, H193, R194, T196, Q197, S204, K210, K211, E212, N215, Q216, V217, L218, L219, E220, A222, I223, L224, Y226, M228, I229, S231, V232, R235, T240, S241, R242, R246, T251, L253, L260, V268, V270, A271, F272, Q275, Y276, D278, S282, E307, E314, R315, D317, A320, I321, D323, M328, K329, C331, F332, L333, A343, D345, N346, K350, G351, E352, P356, Y357, K360, A361, A363, D364, C366, N367, A368, F369, L370, Q371, N378, K379, S380, T383, D386, G389, N390, W392, K393, V402, Y405, V408, V409, Q410, K413, K414, E418, K422, Y423, H424, D425, T426, S428, R429, S431, H432, C437, S444, I447, A448, S457, M460, R461, T462, K463, G464, S466, E467, E468, L469, T471, E472, M475, K484, K489, L490, G491, G492, S493, L494, K497, V500, E501, T502, A503, I504, L506, Q509, S510, H511, T513, H515, G517, A519, S522, R528, K529, V531, V534, I535, T536, E537, I539, L540, F542, and R544.
In other embodiments, the substitution is at a residue selected from the group consisting of: E2H or I or S, A3E or G or K or N or Q or R or T, S13Q or T, D17E, Y18F or M or N, L19F, L20I or V, D23T, D25A or E or S, E26G or N or Q or T, S27E or F or K or V, 128E or F or M or P, E29D or P or R or T, V30N or Q, Y31Q or W, K32D or G or N or R, D33N, K34D or E or Q or S, K36F or R, K37F or I, A40C or D or E or F or M or N or P or Q or V, E41C or D or F or N or Q or S or V, V42A or S or T, R43I or Q, R44A or D or K or M or Y, E45C or M or N or Q, I46F or V, N47E or I or K or R or V, N48A or C or E or F or L or Q or R or S, E49G or H or I or R or S or W, K50C or G or M or N or P or R, A51E or G or L or Q or T, F53D, L54A or C or E or H or I or Q, T55A or H or N or Q or S or Y, L56H or Q or R or S, L57I, L59F or M or S or V or Y, I60C or V, N62V, R71I, E73D, S74G or M or P, D75E, R77A or N or T or V, G78E or I or K or N or P or Q or V or W, A79M or R or Y, D81C or E or H or L or N, R82C or F or G or L or W, F83G or H or I or L or V, V84F or H or L or N or Q or R or S or T or W or Y, S85C or L or N or R, S86C or N, G87C or E or F or K or L or N or T, G88C or D or I or V or W or Y, F89C or I, A91C or D or E or G or H or L or R or S or T or V or Y, V92A or C or E or F or G or I or L or Q or W, T93H or I or Q or V or W, K94C or V or Y, T95C or H or K or M, L97A or M or P, H98C or S or T or V or W, G99A or C or H or P or Q or T, T100A or I or L or M or V, A101S, L102M, S103A or C or G or L, L107C or F, Q109C or N or S, G111A, E113C or H or V, V114C, S115D or Y, Q116G or H or L or S or T or V, E117A or D or I, A118I or V, F119L or M, S120A or D or E or F or K or N or R or W or Y, G121D or L or V or W, K123I or S or W or Y, D124C or E, Q125A or D or G or H or K or L or N or S or T or V or W, G127D or F or W, N128A, F129L or Y, L130A or C or D or Q or V or Y, E131D or F or G or R, L133E or G or I or P or Q or T or V or Y, K134D or G or H or I or L or N or R or W or Y, E135H or S, D136N, I137A or C or D or G or P or Q or S or V, K138C or D or E or P or R or S or V, A139P or S or T or V, I140N or Q or S or T or V, L143A or F or G or N or R or W, A146M, L151E or G or M or N or Q or R or S or T or V or W, E152A or D or I or M or P, G153D, N155E or K or M, I156E or K or L or R or Y, D158E, A160F or H or S, K161L or R or S or Y, V162D or F or N or P or T, F163C or H or I or M or V or W or Y, S166C or E or H or K or P or Q or V or W, H167C or L or P, K169E or G or R, E170G or I or N or R, L171C or E or G or I or M or W, S172G or N or Q or R, K175A or G or H or N or P or T or V, I176A or C or N or Q or V, G177D or E or H or N or P or T, K178D or E or G or I or L or M or N or P or Q or V or Y, E179G or I or P or Q or S or T or V or W or Y, L180F or H or V or W, A181F or M or N or W, E182H or N, Q183A or L, N185D, A187C or S, H193W, R194I, T196V, Q197G, S204A or F or M or W or Y, K210M, K211D or E or F or G or H or I or M or R or S or T or V, E212A or D or M or P or Q or T, N215D or Y, Q216A or E or N, V217C or E or K or N or P or Q or T, L218V, L219I or M or V, E220D or N, A222S, I223C, L224A or C or T or V, Y226F, M228H or R, I229A, S231D or G or H or R or V, V232Q, R235A or D or N, T240V, S241C, R242K or L, R246H or Q, T251H, L253M, L260M, V268I, V270I, A271S, F272Q, Q275E, Y276F or H or Q, D278L or M or R or Y, S282C, E307Q or R, E314H, R315G or K, D317S, A320N or T, I321L or M, D323I or T, M328L, K329G or Q or R, C331T, F332Y, L333F, A343I or V, D345Y, N346A, K350H or W or Y, G351E or M, E352F or I or M or V, P356M or S, Y357E, K360Q, A361Q or S or V, A363S, D364N or T, C366A, N367D or E or M, A368D or Q, F369H or Q, L370A or D or E or F or H or N or R or S or T or V, Q371G or H or I or N or P or R or T or W or Y, N378D, K379E or R or S, S380K or N, T383Q, D386K or S, G389C or M or P or R or T, N390S, W392F or M, K393H or R, V402F or I or L, Y405F, V408Q or S, V409C or Q or S, Q410E or G or H or I or R, K413P, K414C or H or I or Q, E418N, K422G or H or Q or R, Y423G, H424D or G or I or S or T, D425P, T426A or M or Q, S428V, R429A or C or D or G or H or K or N, S431G, H432A or M, C437N, S444N or Q or T, I447K or R, A448H or S or T, S457D, M460A or E or G, R461N, T462S, K463G or N, G464A or D or E or F or H or V or Y, S466E or G or K or N or T, E467N, E468A or N or P or Q, L469A or N, T471N, E472A or G or N, M475I, K484A, K489R, L490I or Y, G491A or C or M or N or Q, G492T or V, S493C or G or K or V, L494G or I or Q or V, K497M or T, V500I or Y, E501N, T502H, A503L or M, I504L, L506 I or V, Q509A, S510T, H511I or M, T513S, H515Q, G517P, A519C, S522A or K, R528K, K529A, V531G or N, V534A or S, I535C or S or T, T536M, E537H or N or Q, I539V, L540E or Q or R or V, F542M, and R544G or N or P or Q or S.
In another aspect of the invention, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that are scored ++ as exemplified in the Examples. These polypeptides can have one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that is indicated in the column labeled ++ as exemplified in the Examples. In another aspect of the invention, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that are scored + as exemplified in the Examples. These polypeptides can have one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that is indicated in the column labeled + as exemplified in the Examples.
In another aspect of the invention, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that has one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that has a rank of 4 as shown in Table 31 of Example 11. In another aspect, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that has one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that has a rank of 3 as shown in Table 31 of Example 11. In another aspect, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that has one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that has a rank of 2 as shown in Table 31 of Example 11. In another aspect, an isoprene synthase variant can be a polypeptide (e.g., an isolated polypeptide) with improved isoprene synthase properties that has one or more substitution(s) at residue positions with numbering that corresponds to SEQ ID NO: 1 (MEA P. alba isoprene synthase) that has a rank of 1 as shown in Table 31 of Example 11.
Exemplary Nucleic Acids
Nucleic acids encoding the isoprene synthase variants of the invention are provided and contemplated within the scope of the invention. In various embodiments, the nucleic acid is a recombinant nucleic acid. For instance, in some embodiments, an isoprene synthase variant nucleic acid is operably linked to another nucleic acid encoding all or a portion of another polypeptide such that the recombinant nucleic acid encodes a fusion polypeptide that includes an isoprene synthase variant and all or part of another polypeptide (e.g., a peptide that facilitates purification or detection of the fusion polypeptide, such as a His-tag). In some embodiments, part or all of a recombinant nucleic acid is chemically synthesized. In some aspects, the nucleic acid is a heterologous nucleic acid. By “heterologous nucleic acid” is meant a nucleic acid whose nucleic acid sequence is not identical to that of another nucleic acid naturally found in the same host cell.
In some embodiments, the nucleic acid includes at least or about 50, 100, 150, 200, 300, 400, 500, 600, 700, 800, or more contiguous nucleotides from a naturally-occurring isoprene synthase nucleic acid. In some aspects, the nucleic acid has one or more mutations compared to the sequence of a wild-type (i.e., a sequence occurring in nature) isoprene synthase nucleic acid. In some embodiments, the nucleic acid has one or more mutations (e.g., a silent mutation) that increase the transcription or translation of isoprene synthase nucleic acid. In some embodiments, the nucleic acid is a degenerate variant of any nucleic acid encoding an isoprene synthase polypeptide.
An isoprene synthase nucleic acid can be incorporated into a vector, such as an expression vector, using standard techniques known to one of skill in the art. Methods used to ligate the DNA construct comprising a nucleic acid of interest such as isoprene synthase, a promoter, a terminator, and other sequences and to insert them into a suitable vector are well known in the art. Additionally, vectors can be constructed using known recombination techniques (e.g., Invitrogen Life Technologies, Gateway Technology).
In some embodiments, it may be desirable to over-express isoprene synthase nucleic acids at levels far higher than currently found in naturally-occurring cells. This result may be accomplished by the selective cloning of the nucleic acids encoding those polypeptides into multicopy plasmids or placing those nucleic acids under a strong inducible or constitutive promoter. Methods for over-expressing desired polypeptides are common and well known in the art of molecular biology and examples may be found in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor, 2001.
Exemplary Pathway Polypeptides
As noted above, one or more polypeptides from the DXP pathway and/or MVA pathway can be used to increase the production of isoprene in conjunction with using the isoprene synthase variants described herein. Accordingly, in certain aspects, the one or more nucleic acids encoding one or more MVA pathway polypeptides is a heterologous nucleic acid. In other aspects, the one or more nucleic acids encoding one or more MVA pathway polypeptides is a copy of an endogenous nucleic acid. In any of the aspects herein, one or more MVA pathway polypeptides can be selected from (a) an enzyme that condenses two molecules of acetyl-CoA to form acetoacetyl-CoA; (b) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA (e.g., HMG synthase); (c) an enzyme that converts HMG-CoA to mevalonate; (d) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate; (e) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; (f) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate; and (g) an enzyme that converts isopentenyl pyrophosphate to dimethylallyl diphosphate. In any of the aspects herein, one or more MVA pathway polypeptides is selected from (a) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA (e.g., HMG synthase); (b) an enzyme that converts HMG-CoA to mevalonate; (c) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate; (d) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; and (e) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate.
In any of the aspects herein, the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate can be selected from the group consisting of M. mazei mevalonate kinase, Lactobacillus mevalonate kinase polypeptide, Lactobacillus sakei mevalonate kinase polypeptide, yeast mevalonate kinase polypeptide, Saccharomyces cerevisiae mevalonate kinase polypeptide, Streptococcus mevalonate kinase polypeptide, Streptococcus pneumoniae mevalonate kinase polypeptide, and Streptomyces mevalonate kinase polypeptide, or Streptomyces CL190 mevalonate kinase polypeptide. In any of the aspects herein, the enzyme that phosphorylates mevalonate to mevalonate 5-phosphate is M. mazei mevalonate kinase.
Upper MVA Pathway Polypeptides
The upper portion of the MVA pathway uses acetyl Co-A produced during cellular metabolism as the initial substrate for conversion to mevalonate via the actions of polypeptides having either: (a) (i) thiolase activity or (ii) acetoacetyl-CoA synthase activity, (b) HMG-CoA reductase, and (c) HMG-CoA synthase enzymatic activity. First, acetyl Co-A is converted to acetoacetyl CoA via the action of a thiolase or an acetoacetyl-CoA synthase (which utilizes acetyl-CoA and malonyl-CoA). Next, acetoacetyl-CoA is converted to 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) by the enzymatic action of HMG-CoA synthase. This Co-A derivative is reduced to mevalonate by HMG-CoA reductase, which is the rate-limiting step of the mevalonate pathway of isoprenoid production.
Non-limiting examples of upper MVA pathway polypeptides include acetyl-CoA acetyltransferase (AA-CoA thiolase) polypeptides, acetoacetyl-CoA synthase polypeptides, 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) polypeptides, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) polypeptides. Upper MVA pathway polypeptides can include polypeptides, fragments of polypeptides, peptides, and fusions polypeptides that have at least one activity of an upper MVA pathway polypeptide. Exemplary upper MVA pathway nucleic acids include nucleic acids that encode a polypeptide, fragment of a polypeptide, peptide, or fusion polypeptide that has at least one activity of an upper MVA pathway polypeptide. Exemplary MVA pathway polypeptides and nucleic acids include naturally-occurring polypeptides and nucleic acids from any of the source organisms described herein. Thus, it is contemplated herein that any gene encoding an upper MVA pathway polypeptide can be used in the present invention.
In certain embodiments, various options of mvaE and mvaS genes from L. grayi, E. faecium, E. gallinarum, E. casseliflavus and/or E. faecalis alone or in combination with one or more other mvaE and mvaS genes encoding proteins from the upper MVA pathway are contemplated within the scope of the invention. In other embodiments, an acetoacetyl-CoA synthase gene is contemplated within the scope of the present invention in combination with one or more other genes encoding: (i) 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) polypeptides and 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) polypeptides. Thus, in certain aspects, any of the combinations of genes contemplated in can be expressed in recombinant cells in any of the ways described herein.
Additional non-limiting examples of upper MVA pathway polypeptides which can be used herein are described in International Patent Application Publication No. WO2009/076676; WO2010/003007 and WO2010/148150.
Genes Encoding mvaE and mvaS Polypeptides
In certain embodiments, various options of mvaE and mvaS genes from L. grayi, E. faecium, E. gallinarum, E. casseliflavus and/or E. faecalis alone or in combination with one or more other mvaE and mvaS genes encoding proteins from the upper MVA pathway are contemplated within the scope of the invention. In L. grayi, E. faecium, E. gallinarum, E. casseliflavus, and E. faecalis, the mvaE gene encodes a polypeptide that possesses both thiolase and HMG-CoA reductase activities. In fact, the mvaE gene product represented the first bifunctional enzyme of IPP biosynthesis found in eubacteria and the first example of HMG-CoA reductase fused to another protein in nature (Hedl, et al., J. Bacteriol. 2002 April; 184(8): 2116-2122). The mvaS gene, on the other hand, encodes a polypeptide having an HMG-CoA synthase activity.
Accordingly, recombinant cells (e.g., E. coli) can be engineered to express one or more mvaE and mvaS genes from L. grayi, E. faecium, E. gallinarum, E. casseliflavus and/or E. faecalis, to produce mevalonate. The one or more mvaE and mvaS genes can be expressed on a multicopy plasmid. The plasmid can be a high copy plasmid, a low copy plasmid, or a medium copy plasmid. Alternatively, the one or more mvaE and mvaS genes can be integrated into the host cell's chromosome. For both heterologous expression of the one or more mvaE and mvaS genes on a plasmid or as an integrated part of the host cell's chromosome, expression of the genes can be driven by either an inducible promoter or a constitutively expressing promoter. The promoter can be a strong driver of expression, it can be a weak driver of expression, or it can be a medium driver of expression of the one or more mvaE and mvaS genes.
In any of the aspects herein, the recombinant host cells can further comprise one or more nucleic acids encoding one or more 1-deoxy-D-xylulose 5-phosphate (DXP) pathway polypeptides. In one aspect, one or more nucleic acids that encode for one or more DXP pathway polypeptides is a heterologous nucleic acid. In another aspect, the one or more nucleic acids encoding one or more DXP pathway polypeptides is a copy of an endogenous nucleic acid. In another aspect, the one or more DXP pathway polypeptides is selected from (a) 1-deoxy-D-xylulose-5-phosphate synthase (DXS), (b) 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), (c) 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (MCT), (d) 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK), (e) 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS), (f) 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (HDS), and (g) 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (HDR). In another aspect, the DXP pathway polypeptide is DXS.
In another aspect, one of skill in the art can use an alternate metabolic process which can potentially produce three molecules of acetyl-CoA from one molecule of glucose using a pathway which does not rely on the Wood-Ljungdahl pathway enzymes. Instead, it makes use of a phosphoketolase enzyme found in certain organisms, particularly among Bifidobacteria [see, for example, Biology of the Prokaryotes (ed. Lengeler, Drews and Schlegel); Blackwell Science, New York, 1999, p. 299-301; Meile et al., J. of Bacteriology, 2001, 183:9, 2929-36; Jeong et al., J. Microbiol. Biotechnol., 2007, 17:5, 822-829]. Phosphoketolase enzymes allow for formation of acetyl-CoA (via acetyl-phosphate) from xylulose 5-phosphate or fructose 6-phosphate rather than through oxidation of pyruvate as in typical metabolism. Increased biosynthesis of acetyl CoA by the use of a phosphoketolase polypeptide can result in increased productivity of the upper mevalonate-dependent biosynthetic pathway which can substantially increase biosynthesis of mevalonate and, consequently, of downstream isoprenoid precursor molecules such as DMAPP and IPP. Standard methods can be used to determine whether a polypeptide has phosphoketolase peptide activity by measuring the ability of the peptide to convert D-fructose 6-phosphate or D-xylulose 5-phosphate into acetyl-P. Acetyl-P can then be converted into ferryl acetyl hydroxamate, which can be detected spectrophotometrically (Meile et al., J. Bact. 183:2929-2936, 2001). Any polypeptide identified as having phosphoketolase peptide activity is suitable for use in the present invention. Exemplary phosphoketolase nucleic acids include, but are not limited to, a phosphoketolase isolated from Lactobacillus reuteri, Bifidobacterium longum, Ferrimonas balearica, Pedobactor saltans, Streptomyces griseus, and/or Nocardiopsis dassonvillei. 
Acetoacetyl-CoA Synthase Gene
In another aspect, acetoacetyl-CoA synthase gene (aka nphT7) can be used. The acetoacetyl-CoA synthase gene is a gene encoding an enzyme having the activity of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and having minimal activity (e.g., no activity) of synthesizing acetoacetyl-CoA from two acetyl-CoA molecules. See, e.g., Okamura et al., PNAS Vol 107, No. 25, pp. 11265-11270 (2010), the contents of which are expressly incorporated herein for teaching about nphT7. An acetoacetyl-CoA synthase gene from an actinomycete of the genus Streptomyces CL190 strain was described in JP Patent Publication (Kokai) No. 2008-61506 A and US2010/0285549. Acetoacetyl-CoA synthase can also be referred to as acetyl CoA:malonyl CoA acyltransferase. A representative acetoacetyl-CoA synthase (or acetyl CoA:malonyl CoA acyltransferase) that can be used is Genbank AB540131.1.
In any of the aspects or embodiments described herein, an enzyme that has the ability to synthesize acetoacetyl-CoA from malonyl-CoA and acetyl-CoA can be used. In certain embodiments described herein, an acetoacetyl-CoA synthase gene derived from an actinomycete of the genus Streptomyces having the activity of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA can be used.
Exemplary Host Cells
A variety of host cells can be used to make a recombinant host cell that can express isoprene synthase variants and to produce isoprene in the methods of the claimed invention. The host cell may be a cell that naturally produces isoprene or a cell that does not naturally produce isoprene. In some embodiments, the host cell naturally produces isoprene using the DXP pathway, and an isoprene synthase variant, DXP pathway polypeptide (e.g., DXS), and/or IDI nucleic acid is added to enhance production of isoprene using this pathway. In some embodiments, the host cell naturally produces isoprene using the MVA pathway, and an isoprene synthase variant and/or one or more MVA pathway nucleic acids are added to enhance production of isoprene using this pathway. In some embodiments, the host cell naturally produces isoprene using the DXP pathway and one or more MVA pathway nucleic acids are added to produce isoprene using part or all of the MVA pathway as well as the DXP pathway. In some embodiments, the host cell naturally produces isoprene using both the DXP and MVA pathways and one or more isoprene synthase variants, DXS, IDI, or MVA pathway nucleic acids are added to enhance production of isoprene by one or both of these pathways.
In some embodiments, the host cell is a yeast, such as Saccharomyces sp., Schizosaccharomyces sp., Pichia sp., Candida sp. or Y. lipolytica. 
In some embodiments, the host cell is a bacterium, such as strains of Bacillus such as B. lichenformis or B. subtilis, strains of Pantoea such as P. citrea, strains of Pseudomonas such as P. alcaligenes, strains of Streptomyces such as S. lividans or S. rubiginosus, strains of Escherichia such as E. coli, strains of Enterobacter, strains of Streptococcus, strains of Archaea such as Methanosarcina mazei or strains of Corynebacterium such as C. glutamicum. 
As used herein, “the genus Bacillus” includes all species within the genus “Bacillus,” as known to those of skill in the art, including but not limited to B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans, B. lautus, and B. thuringiensis. It is recognized that the genus Bacillus continues to undergo taxonomical reorganization. Thus, it is intended that the genus include species that have been reclassified, including but not limited to such organisms as B. stearothermophilus, which is now named “Geobacillus stearothermophilus.” The production of resistant endospores in the presence of oxygen is considered the defining feature of the genus Bacillus, although this characteristic also applies to the recently named Alicyclobacillus, Amphibacillus, Aneurinibacillus, Anoxybacillus, Brevibacillus, Filobacillus, Gracilibacillus, Halobacillus, Paenibacillus, Salibacillus, Thermobacillus, Ureibacillus, and Virgibacillus. 
In some embodiments, the host cell is a gram-positive bacterium. Non-limiting examples include strains of Streptomyces (e.g., S. lividans, S. coelicolor, or S. griseus) and Bacillus. In some embodiments, the source organism is a gram-negative bacterium, such as E. coli or Pseudomonas sp.
In some embodiments, the host cell is a plant, such as a plant from the family Fabaceae, such as the Faboideae subfamily. In some embodiments, the source organism is kudzu, poplar (such as Populus alba x tremula CAC35696), aspen (such as Populus tremuloides), or Quercus robur. 
In some embodiments, the host cell is an algae, such as a green algae, red algae, glaucophytes, chlorarachniophytes, euglenids, chromista, or dinoflagellates.
In some embodiments, the host cell is a cyanobacteria, such as cyanobacteria classified into any of the following groups based on morphology: Chroococcales, Pleurocapsales, Oscillatoriales, Nostocales, or Stigonematales.
In some embodiments, the host cell is an anaerobic organisms. An “anaerobe” is an organism that does not require oxygen for growth. An anaerobe can be an obligate anaerobe, a facultative anaerobe, or an aerotolerant organism. Such organisms can be any of the organisms listed above, bacteria, yeast, etc. An “obligate anaerobe” is an anaerobe for which atmospheric levels of oxygen can be lethal. Examples of obligate anaerobes include, but are not limited to, Clostridium, Eurobacterium, Bacteroides, Peptostreptococcus, Butyribacterium, Veillonella, and Actinomyces. In one embodiment, the obligate anaerobes can be any one or combination selected from the group consisting of Clostridium ljungdahlii, Clostridium autoethanogenum, Eurobacterium limosum, Clostridium carboxydivorans, Peptostreptococcus productus, and Butyribacterium methylotrophicum. A “facultative anaerobe” is an anaerobe that is capable of performing aerobic respiration in the presence of oxygen and is capable of performing anaerobic fermentation under oxygen-limited or oxygen-free conditions. Examples of facultative anaerobes include, but are not limited to, Escherichia, Pantoea, yeast, and Yarrowia. 
In some embodiments, the host cell is a photosynthetic cell. In other embodiments, the host cell is a non-photosynthetic cell.
Other exemplary host cells that can be used are described in US Pub. 2009/0203102, WO 2009/076676, WO 2010/003007, WO 2009/132220, WO 2010/031062, WO 2010/031068, WO 2010/031076, WO 2010/031077, and WO 2010/031079.
Exemplary Transformation Methods
Isoprene synthase, DXS, IDI, and/or MVA pathway nucleic acids or vectors containing them can be inserted into a host cell (e.g., a bacterial cell) using standard techniques for expression of the encoded isoprene synthase, DXS, IDI, and/or MVA pathway polypeptide. Introduction of a DNA construct or vector into a host cell can be performed using techniques such as transformation, electroporation, nuclear microinjection, transduction, transfection (e.g., lipofection mediated or DEAE-Dextrin mediated transfection or transfection using a recombinant phage virus), incubation with calcium phosphate DNA precipitate, high velocity bombardment with DNA-coated microprojectiles, and protoplast fusion. General transformation techniques are known in the art (see, e.g., Current Protocols in Molecular Biology (F. M. Ausubel et al. (eds) Chapter 9, 1987; Sambrook et al., Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor, 2001; and Campbell et al., Curr Genet, 16:53-56, 1989, which are each hereby incorporated by reference in their entireties, particularly with respect to transformation methods). The introduced nucleic acids may be integrated into chromosomal DNA or maintained as extrachromosomal replicating sequences.
Other exemplary transformation methods that can be used are described in US Pub. 2009/0203102, WO 2009/076676, WO 2010/003007, WO 2009/132220, WO 2010/031062, WO 2010/031068, WO 2010/031076, WO 2010/031077, and WO 2010/031079.
Exemplary Cell Culture Media
Any carbon source can be used to cultivate the host cells. The term “carbon source” refers to one or more carbon-containing compounds capable of being metabolized by a host cell or organism. For example, the cell medium used to cultivate the host cells may include any carbon source suitable for maintaining the viability or growing the host cells.
In some embodiments, the carbon source is a carbohydrate (such as monosaccharide, disaccharide, oligosaccharide, or polysaccharides), invert sugar (e.g., enzymatically treated sucrose syrup), glycerol, glycerine (e.g., a glycerine byproduct of a biodiesel or soap-making process), dihydroxyacetone, one-carbon source, fatty acid (e.g., a saturated fatty acid, unsaturated fatty acid, or polyunsaturated fatty acid), lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, polypeptide (e.g., a microbial or plant protein or peptide), renewable carbon source (e.g., a biomass carbon source such as a hydrolyzed biomass carbon source; beet sugar or cane sugar molasses), yeast extract, component from a yeast extract, polymer, acid, alcohol, aldehyde, ketone, amino acid, succinate, lactate, acetate, ethanol, or any combination of two or more of the foregoing. In some embodiments, the carbon source is a product of photosynthesis, including, but not limited to, glucose.
Exemplary monosaccharides include glucose and fructose; exemplary oligosaccharides include lactose and sucrose, and exemplary polysaccharides include starch and cellulose. Exemplary carbohydrates include C6 sugars (e.g., fructose, mannose, galactose, or glucose) and C5 sugars (e.g., xylose or arabinose). In some embodiments, the cell medium includes a carbohydrate as well as a carbon source other than a carbohydrate (e.g., glycerol, glycerine, dihydroxyacetone, one-carbon source, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, or a component from a yeast extract). In some embodiments, the cell medium includes a carbohydrate as well as a polypeptide (e.g., a microbial or plant protein or peptide). In some embodiments, the microbial polypeptide is a polypeptide from yeast or bacteria. In some embodiments, the plant polypeptide is a polypeptide from soy, corn, canola, jatropha, palm, peanut, sunflower, coconut, mustard, rapeseed, cottonseed, palm kernel, olive, safflower, sesame, or linseed.
In some embodiments, the concentration of the carbohydrate is at least or about 5 grams per liter of broth (g/L, wherein the volume of broth includes both the volume of the cell medium and the volume of the cells), such as at least or about 10, 15, 20, 30, 40, 50, 60, 80, 100, 150, 200, 300, 400, or more g/L. In some embodiments, the concentration of the carbohydrate is between about 50 and about 400 g/L, such as between about 100 and about 360 g/L, between about 120 and about 360 g/L, or between about 200 and about 300 g/L. In some embodiments, this concentration of carbohydrate includes the total amount of carbohydrate that is added before and/or during the culturing of the host cells.
Exemplary lipids are any substance containing one or more fatty acids that are C4 and above fatty acids that are saturated, unsaturated, or branched.
Exemplary fatty acids include compounds of the formula R—COOH, where “R” is a hydrocarbon. Exemplary unsaturated fatty acids include compounds where “R” includes at least one carbon-carbon double bond. Exemplary unsaturated fatty acids include, but are not limited to, oleic acid, vaccenic acid, linoleic acid, palmitelaidic acid, and arachidonic acid. Exemplary polyunsaturated fatty acids include compounds where “R” includes a plurality of carbon-carbon double bonds. Exemplary saturated fatty acids include compounds where “R” is a saturated aliphatic group. In some embodiments, the carbon source includes one or more C12-C22 fatty acids, such as a C12 saturated fatty acid, a C14 saturated fatty acid, a C16 saturated fatty acid, a C18 saturated fatty acid, a C20 saturated fatty acid, or a C22 saturated fatty acid. In an exemplary embodiment, the fatty acid is palmitic acid. In some embodiments, the carbon source is a salt of a fatty acid (e.g., an unsaturated fatty acid), a derivative of a fatty acid (e.g., an unsaturated fatty acid), or a salt of a derivative of fatty acid (e.g., an unsaturated fatty acid). Suitable salts include, but are not limited to, lithium salts, potassium salts, sodium salts, and the like. Di- and triglycerols are fatty acid esters of glycerol.
In some embodiments, the concentration of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride is at least or about 1 gram per liter of broth (g/L, wherein the volume of broth includes both the volume of the cell medium and the volume of the cells), such as at least or about 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, 150, 200, 300, 400, or more g/L. In some embodiments, the concentration of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride is between about 10 and about 400 g/L, such as between about 25 and about 300 g/L, between about 60 and about 180 g/L, or between about 75 and about 150 g/L. In some embodiments, the concentration includes the total amount of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride that is added before and/or during the culturing of the host cells. In some embodiments, the carbon source includes both (i) a lipid, fatty acid, monoglyceride, diglyceride, or triglyceride and (ii) a carbohydrate, such as glucose. In some embodiments, the ratio of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride to the carbohydrate is about 1:1 on a carbon basis (i.e., one carbon in the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride per carbohydrate carbon). In particular embodiments, the amount of the lipid, fatty acid, monoglyceride, diglyceride, or triglyceride is between about 60 and 180 g/L, and the amount of the carbohydrate is between about 120 and 360 g/L.
Exemplary microbial polypeptide carbon sources include one or more polypeptides from yeast or bacteria. Exemplary plant polypeptide carbon sources include one or more polypeptides from soy, corn, canola, jatropha, palm, peanut, sunflower, coconut, mustard, rapeseed, cottonseed, palm kernel, olive, safflower, sesame, or linseed.
Exemplary renewable carbon sources include cheese whey permeate, cornsteep liquor, sugar beet molasses, barley malt, and components from any of the foregoing. Exemplary renewable carbon sources also include glucose, hexose, pentose and xylose present in biomass, such as corn, switchgrass, sugar cane, cell waste of fermentation processes, and protein by-product from the milling of soy, corn, or wheat. In some embodiments, the biomass carbon source is a lignocellulosic, hemicellulosic, or cellulosic material such as, but are not limited to, a grass, wheat, wheat straw, bagasse, sugar cane bagasse, soft wood pulp, corn, corn cob or husk, corn kernel, fiber from corn kernels, corn stover, switch grass, rice hull product, or a by-product from wet or dry milling of grains (e.g., corn, sorghum, rye, triticate, barley, wheat, and/or distillers grains). Exemplary cellulosic materials include wood, paper and pulp waste, herbaceous plants, and fruit pulp. In some embodiments, the carbon source includes any plant part, such as stems, grains, roots, or tubers. In some embodiments, all or part of any of the following plants are used as a carbon source: corn, wheat, rye, sorghum, triticate, rice, millet, barley, cassava, legumes, such as beans and peas, potatoes, sweet potatoes, bananas, sugarcane, and/or tapioca. In some embodiments, the carbon source is a biomass hydrolysate, such as a biomass hydrolysate that includes both xylose and glucose or that includes both sucrose and glucose.
In some embodiments, the renewable carbon source (such as biomass) is pretreated before it is added to the cell culture medium. In some embodiments, the pretreatment includes enzymatic pretreatment, chemical pretreatment, or a combination of both enzymatic and chemical pretreatment (see, for example, Farzaneh et al., Bioresource Technology 96 (18): 2014-2018, 2005; U.S. Pat. No. 6,176,176; U.S. Pat. No. 6,106,888). In some embodiments, the renewable carbon source is partially or completely hydrolyzed before it is added to the cell culture medium.
In some embodiments, the renewable carbon source (such as corn stover) undergoes ammonia fiber expansion (AFEX) pretreatment before it is added to the cell culture medium (see, for example, Farzaneh et al., Bioresource Technology 96 (18): 2014-2018, 2005). During AFEX pretreatment, a renewable carbon source is treated with liquid anhydrous ammonia at moderate temperatures (such as about 60 to about 100° C.) and high pressure (such as about 250 to about 300 psi) for about 5 minutes. Then, the pressure is rapidly released. In this process, the combined chemical and physical effects of lignin solubilization, hemicellulose hydrolysis, cellulose decrystallization, and increased surface area enables near complete enzymatic conversion of cellulose and hemicellulose to fermentable sugars. AFEX pretreatment has the advantage that nearly all of the ammonia can be recovered and reused, while the remaining serves as nitrogen source for microbes in downstream processes. Also, a wash stream is not required for AFEX pretreatment. Thus, dry matter recovery following the AFEX treatment is essentially 100%. AFEX is basically a dry-to-dry process. The treated renewable carbon source is stable for long periods and can be fed at very high solid loadings in enzymatic hydrolysis or fermentation processes. Cellulose and hemicellulose are well preserved in the AFEX process, with little or no degradation. There is no need for neutralization prior to the enzymatic hydrolysis of a renewable carbon source that has undergone AFEX pretreatment. Enzymatic hydrolysis of AFEX-treated carbon sources produces clean sugar streams for subsequent fermentation use.
In some embodiments, the concentration of the carbon source (e.g., a renewable carbon source) is equivalent to at least or about 0.1, 0.5, 1, 1.5 2, 3, 4, 5, 10, 15, 20, 30, 40, or 50% glucose (w/v). The equivalent amount of glucose can be determined by using standard HPLC methods with glucose as a reference to measure the amount of glucose generated from the carbon source. In some embodiments, the concentration of the carbon source (e.g., a renewable carbon source) is equivalent to between about 0.1 and about 20% glucose, such as between about 0.1 and about 10% glucose, between about 0.5 and about 10% glucose, between about 1 and about 10% glucose, between about 1 and about 5% glucose, or between about 1 and about 2% glucose.
In some embodiments, the carbon source includes yeast extract or one or more components of yeast extract. In some embodiments, the concentration of yeast extract is at least 1 gram of yeast extract per liter of broth (g/L, wherein the volume of broth includes both the volume of the cell medium and the volume of the cells), such at least or about 5, 10, 15, 20, 30, 40, 50, 60, 80, 100, 150, 200, 300, or more g/L. In some embodiments, the concentration of yeast extract is between about 1 and about 300 g/L, such as between about 1 and about 200 g/L, between about 5 and about 200 g/L, between about 5 and about 100 g/L, or between about 5 and about 60 g/L. In some embodiments, the concentration includes the total amount of yeast extract that is added before and/or during the culturing of the host cells. In some embodiments, the carbon source includes both yeast extract (or one or more components thereof) and another carbon source, such as glucose. In some embodiments, the ratio of yeast extract to the other carbon source is about 1:5, about 1:10, or about 1:20 (w/w).
Additionally the carbon source may also be one-carbon substrates such as carbon dioxide, or methanol. Glycerol production from single carbon sources (e.g., methanol, formaldehyde, or formate) has been reported in methylotrophic yeasts (Yamada et al., Agric. Biol. Chem., 53(2) 541-543, I989) and in bacteria (Hunter et. al., Biochemistry, 24, 4148-4155, I985). These organisms can assimilate single carbon compounds, ranging in oxidation state from methane to formate, and produce glycerol. The pathway of carbon assimilation can be through ribulose monophosphate, through serine, or through xylulose-momophosphate (Gottschalk, Bacterial Metabolism, Second Edition, Springer-Verlag: New York, 1986, which is hereby incorporated by reference in its entirety, particularly with respect to carbon sources). The ribulose monophosphate pathway involves the condensation of formate with ribulose-5-phosphate to form a six carbon sugar that becomes fructose and eventually the three carbon product glyceraldehyde-3-phosphate. Likewise, the serine pathway assimilates the one-carbon compound into the glycolytic pathway via methylenetetrahydrofolate.
In addition to one and two carbon substrates, methylotrophic organisms are also known to utilize a number of other carbon containing compounds such as methylamine, glucosamine and a variety of amino acids for metabolic activity. For example, methylotrophic yeast are known to utilize the carbon from methylamine to form trehalose or glycerol (Bellion et al., Microb. Growth Cl Compd., Int. Symp., 7th ed., 415-32. Editors: Murrell et al., Publisher: Intercept, Andover, UK, 1993). Similarly, various species of Candida metabolize alanine or oleic acid (Sulter et al., Arch. Microbiol. 153(5), 485-9, 1990).
In some embodiments, cells are cultured in a standard medium containing physiological salts and nutrients (see, e.g., Pourquie, J. et al., Biochemistry and Genetics of Cellulose Degradation, eds. Aubert et al., Academic Press, pp. 71-86, 1988; and Ilmen et al., Appl. Environ. Microbiol. 63:1298-1306, I997). Exemplary growth media are common commercially prepared media such as Luria Bertani (LB) broth, Sabouraud Dextrose (SD) broth, or Yeast medium (YM) broth. One skilled in the art of microbiology or fermentation science would know other defined or synthetic growth media that may also be used, and the appropriate medium for growth of particular host cells.
In addition to an appropriate carbon source, the cell medium desirably contains suitable minerals, salts, cofactors, buffers, and other components known to those skilled in the art suitable for the growth of the cultures or the enhancement of isoprene production (see, for example, WO 2004/033646 and references cited therein and WO 96/35796 and references cited therein). In some embodiments where an isoprene synthase, DXS, IDI, and/or MVA pathway nucleic acid is under the control of an inducible promoter, the inducing agent (e.g., a sugar, metal salt or antimicrobial), is desirably added to the medium at a concentration effective to induce expression of an isoprene synthase, DXS, IDI, and/or MVA pathway polypeptide. In some embodiments, cell medium has an antibiotic (such as kanamycin) that corresponds to the antibiotic resistance nucleic acid (such as a kanamycin resistance nucleic acid) on a vector that has one or more DXS, IDI, or MVA pathway nucleic acids.
Other exemplary cell culture media that can be used are described in US Pub. 2009/0203102, WO 2009/076676, WO 2010/003007, WO 2009/132220, WO 2010/031062, WO 2010/031068, WO 2010/031076, WO 2010/031077, and WO 2010/031079.
Exemplary Production of Isoprene
In some embodiments, the cells are cultured in a culture medium under conditions permitting the production of isoprene by the cells. In some embodiments, the cells in culture produce isoprene at greater than or about 1, 10, 25, 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,250, 1,500, 1,750, 2,000, 2,500, 3,000, 4,000, 5,000, or more nmole of isoprene/gram of cells for the wet weight of the cells/hour (nmole/gwcm/hr). In some embodiments, the amount of isoprene is between about 2 to about 5,000 nmole/gwcm/hr, such as between about 2 to about 100 nmole/gwcm/hr, about 100 to about 500 nmole/gwcm/hr, about 150 to about 500 nmole/gwcm/hr, about 500 to about 1,000 nmole/gwcm/hr, about 1,000 to about 2,000 nmole/gwcm/hr, or about 2,000 to about 5,000 nmole/gwcm/hr. The amount of isoprene in units of nmole/gwcm/hr can be measured as disclosed in U.S. Pat. No. 5,849,970. For example, two mL of headspace (e.g., headspace from a culture such as 2 mL of culture cultured in sealed vials at 32° C. with shaking at 200 rpm for approximately 3 hours) are analyzed for isoprene using a standard gas chromatography system, such as a system operated isothermally (85° C.) with an n-octane/porasil C column (Alltech Associates, Inc., Deerfield, Ill.) and coupled to a RGD2 mercuric oxide reduction gas detector (Trace Analytical, Menlo Park, Calif.) (see, e.g., Greenberg et al, Atmos. Environ. 27A: 2689-2692, 1993; Silver et al., Plant Physiol. 97:1588-1591, 1991). The gas chromatography area units are converted to nmol isoprene via a standard isoprene concentration calibration curve. In some embodiments, the value for the grams of cells for the wet weight of the cells is calculated by obtaining the A600 value for a sample of the cell culture, and then converting the A600 value to grams of cells based on a calibration curve of wet weights for cell cultures with a known A600 value. In some embodiments, the grams of the cells is estimated by assuming that one liter of broth (including cell medium and cells) with an A600 value of 1 has a wet cell weight of 1 gram. The value is also divided by the number of hours the culture has been incubating for, such as three hours.
In some embodiments, the cells in culture produce isoprene at greater than or about 1, 10, 25, 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,250, 1,500, 1,750, 2,000, 2,500, 3,000, 4,000, 5,000, 10,000, 100,000, or more ng of isoprene/gram of cells for the wet weight of the cells/hr (ng/gwcm/h). In some embodiments, the amount of isoprene is between about 2 to about 5,000 ng/gwcm/h, such as between about 2 to about 100 ng/gwcm/h, about 100 to about 500 ng/gwcm/h, about 500 to about 1,000 ng/gwcm/h, about 1,000 to about 2,000 ng/gwcm/h, or about 2,000 to about 5,000 ng/gwcm/h. The amount of isoprene in ng/gwcm/h can be calculated by multiplying the value for isoprene production in the units of nmole/gwcm/hr discussed above by 68.1 (as described in Equation 5 below).
In some embodiments, the cells in culture produce a cumulative titer (total amount) of isoprene at greater than or about 1, 10, 25, 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1,000, 1,250, 1,500, 1,750, 2,000, 2,500, 3,000, 4,000, 5,000, 10,000, 50,000, 100,000, or more mg of isoprene/L of broth (mg/L broth, wherein the volume of broth includes the volume of the cells and the cell medium). In some embodiments, the amount of isoprene is between about 2 to about 5,000 mg/L broth, such as between about 2 to about 100 mg/L broth, about 100 to about 500 mg/L broth, about 500 to about 1,000 mg/L broth, about 1,000 to about 2,000 mg/L broth, or about 2,000 to about 5,000 mg/L broth. The specific productivity of isoprene in mg of isoprene/L of headspace from shake flask or similar cultures can be measured by taking a 1 ml sample from the cell culture at an OD600 value of approximately 1.0, putting it in a 20 mL vial, incubating for 30 minutes, and then measuring the amount of isoprene in the headspace. If the OD600 value is not 1.0, then the measurement can be normalized to an OD600 value of 1.0 by dividing by the OD600 value. The value of mg isoprene/L headspace can be converted to mg/Lbroth/hr/OD600 of culture broth by multiplying by a factor of 38. The value in units of mg/Lbroth/hr/OD600 can be multiplied by the number of hours and the OD600 value to obtain the cumulative titer in units of mg of isoprene/L of broth.
The instantaneous isoprene production rate in mg/L broth/hr in a fermentor can be measured by taking a sample of the fermentor off-gas, analyzing it for the amount of isoprene (in units such as mg of isoprene per L of gas), and multiplying this value by the rate at which off-gas is passed though each liter of broth (e.g., at 1 vvm (volume of air/volume of broth/minute) this is 60 Lgas per hour). Thus, an off-gas level of 1 mg/Lgas corresponds to an instantaneous production rate of 60 mg/Lbroth/hr at air flow of 1 vvm. If desired, the value in the units mg/Lbroth/hr can be divided by the OD600 value to obtain the specific rate in units of mg/Lbroth/hr/OD. The average value of mg isoprene/Lgas can be converted to the total product productivity (grams of isoprene per liter of fermentation broth, mg/Lbroth) by multiplying this average off-gas isoprene concentration by the total amount of off-gas sparged per liter of fermentation broth during the fermentation. Thus, an average off-gas isoprene concentration of 0.5 mg/Lbroth/hr over 10 hours at 1 vvm corresponds to a total product concentration of 300 mg isoprene/Lbroth.
In some embodiments, the cells in culture convert greater than or about 0.0015, 0.002, 0.005, 0.01, 0.02, 0.05, 0.1, 0.12, 0.14, 0.16, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.2, 1.4, or 1.6% of the carbon in the cell culture medium into isoprene. In some embodiments, the percent conversion of carbon into isoprene is between about 0.002 to about 1.6%, such as about 0.002 to about 0.005%, about 0.005 to about 0.01%, about 0.01 to about 0.05%, about 0.05 to about 0.15%, 0.15 to about 0.2%, about 0.2 to about 0.3%, about 0.3 to about 0.5%, about 0.5 to about 0.8%, about 0.8 to about 1.0%, or about 1.0 to about 1.6%. The percent conversion of carbon into isoprene (also referred to as “% carbon yield”) can be measured by dividing the moles carbon in the isoprene produced by the moles carbon in the carbon source (such as the moles of carbon in batched and fed glucose and yeast extract). This number is multiplied by 100% to give a percentage value (as indicated in Equation 1).

% Carbon Yield=(moles carbon in isoprene produced)/(moles carbon in carbon source)*100  Equation 1

For this calculation, yeast extract can be assumed to contain 50% w/w carbon.

% Carbon Yield=(39.1 g isoprene*1/68.1 mol/g*5 C/mol)/[(181221 g glucose*1/180 mol/g*6 C/mol)+(17780 g yeast extract*0.5*1/12 mol/g)]*100=0.042%  Equation 2

One skilled in the art can readily convert the rates of isoprene production or amount of isoprene produced into any other units. Exemplary equations are listed below for interconverting between units.
Units for Rate of Isoprene Production (Total and Specific)

1 g isoprene/Lbroth/hr=14.7 mmol isoprene/Lbroth/hr(total volumetric rate)  Equation 3

1 nmol isoprene/gwcm/hr=1 nmol isoprene/Lbrothhr/OD600(This conversion assumes that one liter of broth with an OD600 value of 1 has a wet cell weight of 1 gram.)  Equation 4

1 nmol isoprene/gwcm/hr=68.1 ng isoprene/gwcm/hr(given the molecular weight of isoprene)  Equation 5

1 nmol isoprene/LgasO2/hr=90 nmol isoprene/Lbroth/hr(at an O2 flow rate of 90 L/hr per L of culture broth)  Equation 6

1 μg isoprene/Lgas isoprene in off-gas=60 μg isoprene/Lbroth/hr at a flow rate of 60 Lgas per Lbroth(1 vvm)  Equation 7

Units for Titer (Total and Specific)

1 nmol isoprene/mg cell protein=150 nmol isoprene/Lbroth/OD600(This conversion assumes that one liter of broth with an OD600 value of 1 has a total cell protein of approximately 150 mg)(specific productivity)  Equation 8

1 g isoprene/Lbroth=14.7 mmol isoprene/Lbroth(total titer)  Equation 9

If desired, Equation 10 can be used to convert any of the units that include the wet weight of the cells into the corresponding units that include the dry weight of the cells.

Dry weight of cells=(wet weight of cells)/3.3  Equation 10

In some embodiments encompassed by the invention, a cell comprising a heterologous nucleic acid encoding an isoprene synthase variant polypeptide produces an amount of isoprene that is at least or about 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold, 100-fold, 150-fold, 200-fold, 400-fold, or greater than the amount of isoprene produced from a corresponding cell grown under essentially the same conditions without the heterologous nucleic acid encoding the isoprene synthase variant polypeptide.
In some embodiments encompassed by the invention, a cell comprising a heterologous nucleic acid encoding an isoprene synthase variant polypeptide and one or more heterologous nucleic acids encoding a DXS, IDI, and/or MVA pathway polypeptide produces an amount of isoprene that is at least or about 2-fold, 3-fold, 5-fold, 10-fold, 25-fold, 50-fold, 100-fold, 150-fold, 200-fold, 400-fold, or greater than the amount of isoprene produced from a corresponding cell grown under essentially the same conditions without the heterologous nucleic acids.
Exemplary Isoprene Purification Methods
In some embodiments, any of the methods described herein further include recovering the isoprene. For example, the isoprene produced using the compositions and methods of the invention can be recovered using standard techniques, such as gas stripping, fractionation, adsorption/desorption, pervaporation, thermal or vacuum desorption of isoprene from a solid phase, or extraction of isoprene immobilized or absorbed to a solid phase with a solvent (see, for example, U.S. Pat. Nos. 4,703,007 and 4,570,029). In some embodiments, the recovery of isoprene involves the isolation of isoprene in a liquid form (such as a neat solution of isoprene or a solution of isoprene in a solvent). Gas stripping involves the removal of isoprene vapor from the fermentation off-gas stream in a continuous manner. Such removal can be achieved in several different ways including, but not limited to, adsorption to a solid phase, partition into a liquid phase, or direct condensation. In some embodiments, membrane enrichment of a dilute isoprene vapor stream above the dew point of the vapor resulting in the condensation of liquid isoprene. In some embodiments, the recovery is performed as described in U.S. Provisional Patent Appl. No. 61/288,142, filed on Dec. 18, 2009.
The recovery of isoprene may involve one step or multiple steps. In some embodiments, the removal of isoprene vapor from the fermentation off-gas and the conversion of isoprene to a liquid phase are performed simultaneously. For example, isoprene can be directly condensed from the off-gas stream to form a liquid. In some embodiments, the removal of isoprene vapor from the fermentation off-gas and the conversion of isoprene to a liquid phase are performed sequentially. For example, isoprene may be adsorbed to a solid phase and then extracted from the solid phase with a solvent.
In some embodiments, any of the methods described herein further include purifying the isoprene. For example, the isoprene produced using the compositions and methods of the invention can be purified using standard techniques. Purification refers to a process through which isoprene is separated from one or more components that are present when the isoprene is produced. In some embodiments, the isoprene is obtained as a substantially pure liquid. Examples of purification methods include (i) distillation from a solution in a liquid extractant and (ii) chromatography. As used herein, “purified isoprene” means isoprene that has been separated from one or more components that are present when the isoprene is produced. See, e.g. U.S. Patent Application Publication No. 2009/0203102, PCT publication WO 2009/076676 and U.S. patent application Ser. No. 12/496,573. In some embodiments, the isoprene is at least about 20%, by weight, free from other components that are present when the isoprene is produced. In various embodiments, the isoprene is at least or about 25%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 90%, 95%, or 99%, by weight, pure. Purity can be assayed by any appropriate method, e.g., by column chromatography, HPLC analysis, or GC-MS analysis.
All publications and patents mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. It is also to be understood that one, some, or all of the properties of the various embodiments described herein may be combined to form other embodiments of the present invention. These and other aspects of the invention will become apparent to one of skill in the art.
EXPERIMENTAL
The following examples are provided in order to demonstrate and further illustrate certain preferred embodiments and aspects of the present invention and are not to be construed as limiting the scope thereof.
In the experimental disclosure which follows, the following abbreviations apply: ° C. (degrees Centigrade); rpm (revolutions per minute); H2O (water); diH2O (deionized water); aa and AA (amino acid); by (base pair); kb (kilobase pair); kD (kilodaltons); gm (grams); μg and ug (micrograms); mg (milligrams); ng (nanograms); μl and ul (microliters); ml (milliliters); mm (millimeters); qs (quantity sufficient); nm (nanometers); μm and um (micrometer); M (molar); mM (millimolar); μM and uM (micromolar); pM (picomolar); U (units); MW (molecular weight); sec (seconds); min (minute/minutes); hr (hour/hours); OD600 (optical density at 600 nm); BSA (bovine serum albumin); DMAPP (dimethylallyl diphosphate); DTT (dithiothreitol); EtOH (ethanol); IPTG (isopropyl-beta-D-thiogalactopyranoside); isoprene(2-methyl-1,3-butadiene); IspS (isoprene synthase); PAGE (polyacrylamide gel electrophoresis); PBS (phosphate buffered saline [150 mM NaCl, 10 mM sodium phosphate buffer, pH 7.2]); and SDS (sodium dodecyl sulfate).
The following abbreviations apply to companies whose products or services may have been referred to in the experimental examples: Agilent (Agilent Technologies, Santa Clara, Calif.); Becton Coulter (Becton Coulter, Inc., Fullerton, Calif.); Bio-Rad (Bio-Rad Laboratories, Hercules, Calif.); Cayman Chemical (Cayman Chemical Co., Ann Arbor, Mich.); CTC Analytics (CTC Analytics A.G., Zwingen, Switzerland); EMS (Electron Microscopy Supply, Hatfield, Pa.); Epicentre (Epicentre Biotechnologies, Madison, Wis.); Integrated DNA Technologies (Integrated DNA Technologies, Coralville, Iowa); Invitrogen (Invitrogen Corp., Carlsbad, Calif.); Molecular Dynamics (Molecular Dynamics, Sunnyvale, Calif.); Novagen (Novagen, Inc., Madison, Wis.); Perkin Elmer (Perkin Elmer, Waltham, Mass.); Roche (Roche Applied Science, Indianapolis, Ind.); Sigma (Sigma-Aldrich, St. Louis, Mo.); Stratagene (Stratagene Cloning Systems, La Jolla, Calif.); Qiagen (Qiagen, Inc., Valencia, Calif.); Takara (Takara Bio USA, Madison, Wis.); Thomson Instrument (Thomson Instrument Co., Oceanside, Calif.); V&P Scientific (V&P Scientific, Inc., San Diego, Calif.); and Zinsser (Zinsser North America, Northridge, Calif.).
EXAMPLES
The following examples are provides for illustrative purposes and are not meant to limit the invention in any manner.
Example 1
Isoprene Synthase Growth Screen: Validation, Optimization and Limitations
This Example describes the development of an in vivo screen to select improved variants of isoprene synthase. The inventors have found that the in vivo screen can be used to select cells that contain less isoprene synthase activity than a control (in our case, the best validated isoprene synthase MEA-Poplar Alba). In addition, the in vivo screen can be used to select cells that contain more isoprene synthase activity than a control (in our case, the best validated isoprene synthase MEA-Poplar Alba).
Methods
Strains: The screening strain contained a constitutively expressed lower pathway and variants of isoprene synthase expressed from a pET plasmid. The screening strains were DW425-positive control.
Assay conditions: Strains were grown overnight in LB medium containing 50 uM kanamycin at 34° C. The overnight cultures were diluted to approximately 0.2 OD600 in TM3 media containing 1% glucose, 0.1% yeast extract, 8 mM MgSO4 and one of the following concentrations of IPTG: 0, 10, 20, 30, 40, 50, 60, or 70 uM. Cells were grown for approximately 2 hours post-induction and transferred to a 96-well clear bottom microtiter plate containing various concentrations of mevalonate (0, 5, 7.5, 10, 15, 20 mM) and the same media used in the day culture to a final OD600 of 0.2-0.3. The plates were monitored in kinetic mode on a Spectramax UV-Vis spectrophotometer. The experiment was monitored at 34 C for 3 hours with shaking for 1 minute prior to each measurement (taken every 5 minutes).
Data analysis: All data were transferred to Excel. The absorbance measurements were converted to their natural log. A line was then fit to the series using the function “LINEST” to yield the exponential growth constant (growth rate).
Metabolite analysis was performed by using the following protocol for methanol/water extraction of metabolites at small-scale (MVA, DXP):
1. Samples from small-scale experiment was quenched; commonly 1 ml sample was spun down, the supernatant was discarded, 100 μl pure methanol was loaded onto the pellet, and the samples was stored at −80C until there was time for metabolite extraction and analysis.
2. Samples were taken from storage in −80C; pellets were resuspended (recommended to break pellet with glass capillary tubes).
3. The sample was spun down in a refrigerated microcentrifuge at 14000 g (rfc) for 4 min.
4. The supernatant was placed into clean 1.5 mL Eppendorf tubes.
5. The pellet was resuspended in 100 μl 6:1 MeOH/5 mM NH4OAc pH 8.0. Centrifuge at 14000 g (rfc) for 4 min. The samples may be extracted in 6:1 MeOH/5 mM NH4OAc pH 7.0 if the metabolites of interest are not stable at pH 8.0 (for example, DXP metabolites, or CoA-containing metabolites).
6. The supernatant was combined with the supernatant from step 4.
7. Steps 5-6 were repeated, extracting with 100 μl 1:1 MeOH/5 mM NH4OAc pH 8.0 (or pH 7.0, see above). Sample pellets can be discarded after taking the supernatant. 1.5 ml Eppendorf tubes containing accumulated supernatant fractions were closed and extract was mixed by vortexing.
8. In order to remove suspended debris, 1.5 ml Eppendorf tubes were centrifuged at 14000 g (rfc) for 4 min.
9.  ̃200 μl extract were placed into LC/MS vials containing conical inserts. The remaining extracts were stored at −20° C.
Without being bound by theory, it is recommended to use repeat pipettors for dispensing 2% formic acid (for fast pipetting and consistent volumes). Repeat pipettors significantly improve time efficiency over standard pipettors, and because they are technically positive displacement pipettes, they are quite precise (and accurate, assuming good calibration and proper maintenance). Further recommendations include, but are not limited to: keep Eppendorf tubes on ice (at 0° C.) whenever possible, the microcentrifuge should be set at −9° C.; allow  ̃20 min. for the centrifuge to cool, for resuspending pellets, the use of the glass capillary tubes is recommended. Mechanical breaking of the cell pellet is usually very fast with just a little physical assistance. It is not recommended to vortex the resuspended pellets, as the cell mass ends up on the sides of the tubes very easily, potentially causing significant experimental error due to the low volumes of the samples.
Without being bound by theory, the following recommendations are given for conducting LC/MS analysis:
1. The LC/MS vials should be kept on the tray at 4° C. during the analysis. The column should be at room temperature. The tray/column temperatures will be set automatically after starting the sequence in Xcalibur, but it is better to set the tray temperature in advance.
2. Use standards prepared as in the attached spreadsheet for calibration. Record standard preparation date as labeled on each tube.
3. LC/MS method for isoprenoids and MVA pathway metabolites (currently on new TSQ Quantum Access)—Method file: IPS_BioBasic100—090316 (or similar, see latest date extension); HPLC column: Macherey-Nagel Nucleodex beta-OH EC 2 mm×100 mm (particle size 5 μm, pore size 100 Å), C/N 720351.20; Guard column: 721460.40 (2 mm guard column not currently available). LC/MS method for DXP pathway metabolites: Method development C18-ion pair\Metabolites_C18_TBAip—11, with tributylammonium acetate as an ion-pair reagent; HPLC column: C18 Phenomenex Synergi 4μ. Hydro-RP 80A 150×2.0 mm, C/N 00F-4375-B0; Guard column: Security Guard Cartridges AQ C18 4×2.0 mm, C/N AJ0-7510. For LC/MS method for detection of CoA-containing metabolites, see “Protocol for acidic extraction of metabolites at small-scale (CoAs, etc.).”
4. After analysis, samples should be stored at −20° C.; standards should be stored at −80° C.
5. Metabolite quantitation can be easily determined using LCQuan software package. After back-calculation of all dilutions (including initial methanol quench), concentrations should be normalized to OD and converted to intracellular concentrations, utilizing the assumption that the intracellular volume of 1 L of fermentation broth at 200 OD is  ̃50 mL.
Results
A system has previously been developed to select DMAPP utilizing enzymes from pools of plasmids that express unknown proteins (Appl Environ Microbiol. 2007 October; 73(19): 6277-6283). The inventors have refined and optimized the screening protocol to enable selection cells that contain isoprene synthase activity. The screen is based on experimental results concluding that the concentration of DMAPP in E. coli correlates with cell growth rate (FIG. 1). Therefore, without being bound by theory, the growth rate of these cells can be thought of as a biosensor for intracellular DMAPP concentrations. Without being bound by theory, the underlying rationale of this screen was that the concentration of DMAPP in a cell can be decreased by increasing the enzyme activity of DMAPP consuming enzymes (isoprene synthase) and, thus, would result in increased growth rate.
To test this hypothesis, DW425 cells were grown in media containing a matrix of IPTG and mevalonate concentrations (FIG. 2 and FIG. 3). Cells grown in the presence of 60 uM IPTG, and greater, resulted in impaired growth without addition of mevalonate (compared to non-induced cells). At IPTG concentrations of 0-50 μM IPTG, growth was unimpaired, compared to non-induced cells. Cell growth was inhibited with all concentrations of mevalonate in uninduced cells. Increasing the concentration of IPTG resulted in increased growth rate for any given mevalonate concentration screened (FIG. 2 and FIG. 3). Earlier studies were performed to determine that the concentration of enzyme expressed in the cells correlates with the concentration of IPTG present. Therefore, increased isoprene synthase expression/activity in these strains results in improved growth.
Example 2
Analysis of P. alba Isoprene Synthase SELs by DMAPP Toxicity Relief
There is a strong correlation between increased intracellular DMAPP levels and growth inhibition of E. coli, which can be alleviated by the expression of P. alba Isoprene Synthase (IspS). Without being bound by theory, increased levels of IspS activity should therefore allow for better growth due to more rapid conversion of DMAPP to isoprene. By monitoring the growth rates of E. coli expressing variants of IspS under these conditions, the inventors can identify mutant IspS enzymes that display increased ability to convert DMAPP to isoprene within the cell.
Methods:
1) Plasmid and Strain Construction:
SEL Plasmid Backbone—The plasmid backbone used to generate SELs was constructed by QuikChange (Stratagene) PCR on the template pDu39 (see Table 1 for primer sequences). The PCR product was treated with 1 μl DpnI (Roche) for 3 hours, and then 1 μl of the entire reaction was transformed into chemically competent E. coli Top10 cells (Invitrogen) according to the manufacturer's recommended protocol. Cells were recovered and plated on LB medium containing 50 μg/ml kanamycin. The next day, positive colonies were chosen for growth, plasmid purification (Qiagen) and sequencing (Quintara Biosciences). Plasmids which harbored the correct base changes were selected for sequencing of the entire open reading frame to confirm the integrity of the coding sequence. One of these plasmids, pCL201 (see FIG. 4), was selected as the backbone for construction of SELs (by Verdezyne and DNA2.0).







TABLE 1





QuikChange and Sequencing Primers
















MEA Hairpin Disrupt (pET) F
ggagatatacatatggaagcacgtcgctctgcgaactacgaacctaa (SEQ ID NO: 2)


MEA Hairpin Disrupt (pET) R
ttaggttcgtagttcgcagagcgacgtgcttccatatgtatatctcc (SEQ ID NO: 3)


 


T7 Forward
taatacgactcactataggg (SEQ ID NO: 4)


T7 Reverse
gctagttattgctcagcgg (SEQ ID NO: 5)


 


EL-1000
gcactgtctttccgtctgctgc (SEQ ID NO: 6)


 


QB1493
cttcggcaacgcatggaaat (SEQ ID NO: 7)


 


A-rev
ctcgtacaggctcaggatag (SEQ ID NO: 8)


 


A-rev2
ttacgtcccaacgctcaact (SEQ ID NO: 9)


 







PCR and cycling parameters:


QuikChange PCR:


 1 ul pDu39


 5 ul 10X PfuUltra HF buffer


 1 ul dNTPs


 1 ul (50uM) MEA Hairpin Disrupt (pET) F


 1 ul (50uM) MEA Hairpin Disrupt (pET) R


 2 ul DMSO


39 ul diH2O


 1 ul PfuUltra HF Polymerase (Stratagene)


PCR Cycling Parameters for QuikChange:


1. 95° C. 1 min.


2. 95° C. 50 sec.


3. 60° C. 50 sec.


4. 68° C. 7 min.


5. Go to step 2-18 cycles


6. 68° C. 7 min





Sequence of pCL201: (SEQ ID NO: 10)


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgcc


 


agcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggc


 


tccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgc


 


cctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccct


 


atctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcga


 


attttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacat


 


tcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaat


 


accatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggt


 


ctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagt


 


gacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaa


 


atcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattac


 


aaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatac


 


ctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaaga


 


ggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactc


 


tggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatc


 


agcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgt


 


aagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaa


 


ggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccgga


 


tcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagtt


 


aggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgata


 


agtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcaca


 


cagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaag


 


ggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacg


 


cctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatg


 


gaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattc


 


tgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcg


 


aggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtac


 


aatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgc


 


caacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgc


 


atgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattca


 


cagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaa


 


gggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagaga


 


ggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgc


 


ggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcag


 


catcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaag


 


accattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaac


 


cagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccg


 


gcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaa


 


taccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcac


 


ctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctga


 


ctgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactg


 


cccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattggg


 


cgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaag


 


cggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatc


 


gtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgat


 


cgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtc


 


gccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacaga


 


acttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgg


 


gagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagca


 


atggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacagg


 


cttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttg


 


cgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcgg


 


ttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgg


 


gaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctctt


 


ccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctg


 


cattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcc


 


caacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatc


 


ttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgt


 


agaggatcgagatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaata


 


attttgtttaactttaagaaggagatatacatatggaagcacgtcgctctgcgaactacgaacctaacagctgggactatgattacct


 


gctgtcctccgacacggacgagtccatcgaagtatacaaagacaaagcgaaaaagctggaagccgaagttcgtcgcgagatta


 


ataacgaaaaagcagaatttctgaccctgctggaactgattgacaacgtccagcgcctgggcctgggttaccgtttcgagtctgat


 


atccgtggtgcgctggatcgcttcgtttcctccggcggcttcgatgcggtaaccaagacttccctgcacggtacggcactgtctttc


 


cgtctgctgcgtcaacacggttttgaggtttctcaggaagcgttcagcggcttcaaagaccaaaacggcaacttcctggagaacc


 


tgaaggaagatatcaaagctatcctgagcctgtacgaggccagcttcctggctctggaaggcgaaaacatcctggacgaggcg


 


aaggttttcgcaatctctcatctgaaagaactgtctgaagaaaagatcggtaaagagctggcagaacaggtgaaccatgcactgg


 


aactgccactgcatcgccgtactcagcgtctggaagcagtatggtctatcgaggcctaccgtaaaaaggaggacgcgaatcag


 


gttctgctggagctggcaattctggattacaacatgatccagtctgtataccagcgtgatctgcgtgaaacgtcccgttggtggcgt


 


cgtgtgggtctggcgaccaaactgcactttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagcattcgaac


 


cgcaatactccgactgccgtaactccgtcgcaaaaatgttttctttcgtaaccattatcgacgatatctacgatgtatacggcaccct


 


ggacgaactggagctgtttactgatgcagttgagcgttgggacgtaaacgccatcaacgacctgccggattacatgaaactgtgc


 


tttctggctctgtataacactattaacgaaatcgcctacgacaacctgaaagataaaggtgagaacatcctgccgtatctgaccaaa


 


gcctgggctgacctgtgcaacgctttcctgcaagaagccaagtggctgtacaacaaatctactccgacctttgacgactacttcgg


 


caacgcatggaaatcctcttctggcccgctgcaactggtgttcgcttacttcgctgtcgtgcagaacattaaaaaggaagagatcg


 


aaaacctgcaaaaataccatgacaccatctctcgtccttcccatatcttccgtctgtgcaatgacctggctagcgcgtctgcggaaa


 


ttgcgcgtggtgaaaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaagaactggctaccgaaagcgtgatg


 


aatctgatcgatgaaacctggaaaaagatgaacaaggaaaaactgggtggtagcctgttcgcgaaaccgttcgtggaaaccgc


 


gatcaacctggcacgtcaatctcactgcacttatcataacggcgacgcgcatacctctccggatgagctgacccgcaaacgcgtt


 


ctgtctgtaatcactgaaccgattctgccgtttgaacgctaaggatccgaattcgagctccgtcgacaagcttgcggccgcactcg


 


agcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagca


 


ataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat


 


Amino Acid Sequence of P. alba IspS: (SEQ ID NO: 1)


MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFL


 


TLLELIDNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQ


 


HGFEVSQEAFSGFKDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAI


 


SHLKELSEEKIGKELAEQVNHALELPLHRRTQRLEAVWSIEAYRKKEDANQVLL


 


ELAILDYNMIQSVYQRDLRETSRWWRRVGLATKLHFARDRLIESFYWAVGVAFE


 


PQYSDCRNSVAKMFSFVTIIDDIYDVYGTLDELELFTDAVERWDVNAINDLPDY


 


MKLCFLALYNTINEIAYDNLKDKGENILPYLTKAWADLCNAFLQEAKWLYNKST


 


PTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIKKEEIENLQKYHDTISRPSHIFRLC


 


NDLASASAEIARGETANSVSCYMRTKGISEELATESVMNLIDETWKKMNKEKLG


 


GSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVITEPILPFER









SEL Expression Host—A P1 lysate of MCM521 (described herein) was made and transduced into BL21(DE3) according to standard molecular biology techniques (Miller, A Short Course in Bacterial Genetics). Transductants were selected on LB medium plates containing 20 μg/ml of kanamycin. Positive colonies were further verified by PCR to confirm the presence of PL.2-mKKDyI in the BL21 DE3 strain. 1 μl of pCP20 plasmid was then transformed into this strain and positive colonies were selected for on LB containing 50 μg/ml carbenicillin and incubated overnight at 30° C. Positive transformants were streaked on an LB plate and incubated at 37° C. to induce loss of the pCP20 plasmid. To confirm the loss of the neomycin (kanamycin) resistance marker, colonies that grew at 37° C. were patched onto LB medium containing either 20 μg/ml of kanamycin, 50 μg/ml of carbenicillin, or no antibiotics. The strains with integrated PL.2 mKKDyI without the kanamycin resistance marker that have lost pCP20 should be sensitive to kanamycin and carbenicillin. Four colonies sensitive to kanamycin and carbenicillin were checked by PCR for the presence of mKKDyI in BL21(DE3) with the parental BL21(DE3) strain as a control. The resulting strain, MD09-170, was used for expression of IspS variants in the growth assay on SELs described below. Control strains for the growth assay harbored either the empty pET24a+ vector or pCL201 as negative and positive controls for IspS expression, respectively (see Table 2).







TABLE 2







Strains









Strain
Plasmid
Description





MD09-170

BL21 (DE3) PL.2-mKKDyI


DW424
pET24a+
BL21 (DE3) PL.2-mKKDyI + vector (negative




control)


DW425
pCL201
BL21 (DE3) PL.2-mKKDyI + P. alba IspS




(wild type control)









2) Construction of SELs:

25 Site Evaluation Libraries (SELs) of IspS were analyzed previously for specific activity. Table 3 lists the residues included in these libraries. For the growth assay, plasmids harboring variant IspS enzymes in these libraries were purified and transformed into the expression host MD09-170: Original libraries were replicated directly from glycerol stocks and grown at 30° C. overnight in LB containing 50 μg/ml kanamycin in 96 deep-well plates (VWR). Cells from the overnight growth were harvested by centrifugation (Eppendorf 5804 R), and supernatants were discarded. Plasmid purification was performed on cell pellets using a Hamilton Microlab STAR robot using the Nucleospin Multi-96 Plus Plasmid purification kit (Macherey Nagel) according to the manufacturers' recommended protocols. 3 μl of the resulting plasmid DNA for each variant was transformed into chemically competent MD09-170 cells in a flat-bottom 96-well polystyrene plate (Falcon) using an Eppendorf Thermomixer R set to 42° C. Cells were recovered for 2 hours in LB medium, and then diluted and incubated overnight in LB medium containing 50 μg/ml kanamycin. Glycerol stocks of plates containing MD09-170 cells with variants from all 25 original libraries were made and stored at −80° C. prior to analysis by growth assay.
A second set of 80 SELs was ordered and manufactured by DNA2.0. These libraries were transformed directly into the screening host MD09-170. Table 4 lists all 80 residues that were chosen for this set. Sites were picked primarily based upon their location in the recently solved crystal structure of P. alba IspS. Strains DW425 and DW424 (see Table 2) were seeded back into 96-well plates for the wild type and negative controls, respectively.







TABLE 3







Sites selected for 25 SELs








Description
Sites





Surface hydrophobic residues
I28, V30, L130, G153, V299, L303,



L469, L494


Hinge region
R198, I229, L260


Negatively charged area
D311, D323


Flexible loops
A443, A453, N454, H515, A519, E525


Active site
F388, N438, E451


Miscellaneous
D345, R528, T536
















TABLE 4







Sites selected for 80 SELs











Position
Residue
Selection Criteria















3
A
surface hydrophobic



7
A
symmetry contact



9
Y
symmetry contact



12
N
conservation



13
S
conservation



16
Y
N-terminal loop



18
Y
N-terminal loop



20
L
conservation



23
D
conservation



25
D
surface hydrophilic



26
E
symmetry contact



27
S
surface hydrophilic



33
D
symmetry contact



36
K
symmetry contact



44
R
symmetry contact



50
K
surface hydrophilic



53
F
conservation



59
L
surface hydrophobic



69
G
conservation



74
S
surface hydrophilic



78
G
conservation



81
D
surface hydrophilic



87
G
surface hydrophobic



99
G
conservation



116
Q
conservation



117
E
symmetry contact



120
S
surface hydrophilic



121
G
surface loop



125
Q
surface hydrophilic



127
G
conservation



139
A
conservation



165
I
surface hydrophobic



173
E
surface hydrophilic



174
E
symmetry contact



177
G
conservation



179
E
surface hydrophilic



194
R
conservation



197
Q
conservation



202
V
conservation



216
Q
conservation



240
T
conservation



246
R
symmetry contact



251
T
surface hydrophilic



254
H
conservation



287
F
active site



290
V
active site



308
L
surface hydrophobic



376
L
flexible loops



377
Y
symmetry contact



379
K
conservation



389
G
conservation



397
G
active site



400
Q
hydrophobic pocket



403
F
active site



421
Q
conservation



426
T
conservation



430
P
misc



434
F
active site



445
A
surface hydrophobic



448
A
conservation



457
S
flexible loops



462
T
conservation



476
N
surface hydrophilic



487
K
surface loop



488
E
surface loop



489
K
surface loop



490
L
surface loop



491
G
surface loop



492
G
surface loop



493
S
surface loop



495
F
surface loop



496
A
surface loop



497
K
surface loop



498
P
conservation



509
Q
conservation



514
Y
active site



521
T
conservation



539
I
C-terminal



540
L
surface hydrophobic



544
R
conservation










3) Growth Assay for Increased IspS Activity

For the growth assay, glycerol stocks of SELs were inoculated into 200 μl LB medium containing 50 μg/ml kanamycin in flat bottom microtiter plates (Cellstar) and grown overnight at 30° C. using the System Duetz (Enzyscreen BV). For pre-induction, 7 μl of the overnight culture from each well was inoculated into 100 μl of TM3 medium containing 50 μM IPTG and 50 μg/ml kanamycin, and plates were grown for 2 hours at 30° C. Pre-induced cultures were then diluted 1:10 into TM3 medium containing 11 mM mevalonic acid, 50 μM IPTG and 50 μg/ml kanamycin in glass-bottom 96 square-well microtiter plates (Matrical). Cultures were grown at 34° C. and shaken at 225 rpm for approximately 10 hours in a Growth Profiler 1152 (Enzyscreen). Growth curves were generated for each IspS variant according to the manufacturer's recommended protocol. Negative controls were strains harboring the empty pET24a+ vector (DW424), and positive controls were strains expressing wild type P. alba IspS (DW425) grown either with or without MVA.
For data analysis, the relative growth rate of each variant over a given period of time was measured against wild type controls. Specifically, a line was fit to the series using the “LINEST” function in Microsoft Excel to yield the exponential growth constant (growth rate). These values were then divided by the average of 4 (in most cases) growth constants from the positive controls to yield a “Growth Index” number for each variant. The Growth Index values for variants in all 105 SEL libraries are listed in Table 5. In some cases, a particular variant was either absent from the glycerol stock, did not grow in the overnight LB culture, or was not transferred to the final plate for growth assay. Values for these particular wells are listed as ND (Not Determined). In the instances where a particular variant was not generated in initial mutagenesis of P. alba IspS, the wild type residue was substituted.










TABLE 5







Variant
GI
















Growth Index Ranking for Plate 001.










V30L
1.61



V30K
1.52



I28T
1.46



V30Y
1.45



V30W
1.39



I28S
1.38



G153W
1.34



I28R
1.31



L130Y
1.29



V30S
1.26



V30V
1.25



V30F
1.22



L130L
1.20



L130K
1.17



I28I
1.15



L130G
1.15



L130I
1.14



V30R
1.13



G153Y
1.12



L130L
1.12



V30I
1.11



I28Y
1.10



L130V
1.09



L130L
1.09



V30P
1.08



V30E
1.07



G153G
1.07



L130R
1.05



V30T
1.05



L130L
1.04



V30Q
1.04



I28P
1.03



G153S
1.02



G153R
1.01



G153C
1.00



L130D
0.97



G153G
0.97



L130E
0.96



G153A
0.96



V30V
0.95



V30M
0.94



I28I
0.92



G153G
0.92



L130L
0.91



G153T
0.90



V30G
0.89



L130W
0.88



L130Q
0.88



G153Q
0.86



G153G
0.86



G153M
0.83



G153N
0.83



G153D
0.81



L130P
0.76



V30N
0.60



I28I
0.57



I28I
0.55



I28L
0.52



I28W
0.42



I28D
0.38



G153K
0.32



G153L
0.32



G153P
0.32



V30C
0.31



G153V
0.31



I28E
0.29



L130A
0.28



I28G
0.28



I28F
0.27



V30A
0.26



I28A
ND



L130M
0.22



I28M
0.15



L130C
0.15



I28N
0.14



I28C
0.10



I28V
ND



V30D
ND



L130S
ND



G153H
ND







Growth Index Ranking for Plate 002.










R198R
1.11



R198R
1.08



I229L
1.00



R198R
0.98



I229C
0.94



L260L
0.89



L260L
0.88



R198R
0.87



I229I
0.86



R198K
0.84



V299V
0.82



I229T
0.80



I229V
0.78



I229M
0.78



L260M
0.77



L260L
0.75



L260W
0.74



R198C
0.74



V299L
0.72



V299V
0.69



R198M
0.68



L260Y
0.66



R198V
0.64



R198A
0.62



I229A
0.62



V299V
0.62



L260Q
0.61



V299V
0.60



I229H
0.57



L260I
0.54



R198I
0.52



I229N
0.49



I229F
0.45



I229S
0.45



L260H
0.45



R198T
0.42



L260P
0.40



L260V
0.38



I229G
0.36



I229Q
0.35



R198S
0.35



L260G
0.34



V299T
0.29



L260C
0.25



V299A
0.25



R198G
0.24



R198N
0.23



L260A
0.22



V299M
0.22



L260S
ND



V299S
0.15



L260D
0.15



V299N
0.14



L260E
0.11



I229Y
0.11



V299R
0.10



R198L
0.10



I229K
0.09



R198F
0.08



I229W
0.08



R198H
0.07



V299E
0.05



V299K
0.03



R198Y
0.03



V299W
0.02



R198D
0.02



I229D
0.02



I229R
0.01



V299Y
0.01



V299G
0.01



I229E
0.01



L260R
0.01



R198P
ND



L260K
0.00



L260T
0.00



V299I
ND



I229P
−0.02



V299H
−0.03



V299P
−0.04



V299D
−0.05







Growth Index Ranking for Plate 003.










L303L
1.10



L303L
1.10



D311D
1.08



L303L
1.06



L303L
0.99



D311W
0.99



D311L
0.98



D311K
0.98



L303W
0.96



D311F
0.93



D311S
0.92



D311G
0.90



D323K
0.89



L303T
0.88



D311R
0.87



L303R
0.85



D311T
0.84



D311V
0.82



L303L
0.81



L303V
0.81



L303I
0.80



D311D
0.80



D311A
0.80



L303S
0.79



D311I
0.78



L303M
0.77



D323L
0.77



D323R
0.75



D311D
0.75



L303E
0.74



D323Y
0.74



L303C
0.74



D323S
0.74



D345L
0.73



D311Q
0.73



L303G
0.72



D323G
0.71



D311D
0.71



D345G
0.70



D323T
0.69



D311E
0.69



D323M
0.69



D323N
0.68



D345W
0.68



D311D
0.67



L303Q
0.67



D345D
0.66



L303A
0.66



D323E
0.66



L303P
0.66



D323A
0.66



D345M
0.66



D345A
0.65



D323D
0.64



D323V
0.63



D323C
0.62



L303H
0.62



L303D
0.62



D323F
0.61



D345S
0.61



D323I
0.61



D345V
0.59



D323Q
0.58



D345T
0.58



D323W
0.57



D345Y
0.57



D345R
0.56



D345N
0.55



D345K
0.53



D345Q
0.53



D345I
0.53



D345D
0.53



D345E
0.52



D345C
0.51



D345D
0.41



D345P
0.37



D311M
ND



D323P
ND



D323H
ND



D311P
−0.02







Growth Index Ranking for Plate 004.










E451E
1.48



A443A
1.36



A443A
1.34



A443A
1.29



F388F
1.28



N438N
1.27



A443A
1.20



F388V
1.20



A443A
1.17



A443A
1.15



A443A
1.13



E451E
1.13



A443A
1.12



F388Q
0.96



F388T
0.89



F388F
0.89



A443S
0.86



A443H
0.85



A443L
0.84



A443N
0.82



F388R
0.74



F388S
0.68



A443Q
0.66



A443I
0.63



A443G
0.50



A443R
0.44



F388D
0.42



A443V
0.40



A443F
0.28



A443P
0.06



E451Q
0.06



A443T
0.03



F388H
ND



F388L
0.00



F388F
−0.01



F388F
−0.01



E451W
−0.01



E451K
−0.01



F388A
−0.02



N438A
−0.02



E451G
−0.02



E451H
−0.02



F388P
−0.02



E451D
−0.02



E451R
−0.03



F388Y
ND



F388K
−0.03



E451Y
−0.03



E451F
−0.03



F388C
−0.03



E451I
−0.03



N438F
−0.04



E451V
−0.04



N438H
−0.04



E451A
−0.04



E451C
−0.04



E451T
−0.04



N438T
−0.04



N438D
−0.04



N438E
−0.04



E451L
−0.05



N438I
−0.05



N438W
ND



E451P
−0.05



N438V
−0.05



F388F
−0.05



N438M
−0.05



N438L
−0.05



N438C
−0.05



N438K
−0.06



E451S
−0.06



F388F
−0.06



E451N
−0.06



N438R
−0.06



N438Y
−0.06



N438Q
−0.06



N438P
−0.06



N438G
−0.07



F388G
−0.08



N438S
−0.09







Growth Index Ranking for Plate 005.










N454N
1.10



L494L
1.06



L469W
1.06



L494K
1.03



L469L
1.00



L469R
0.98



L469L
0.98



L494L
0.98



L494W
0.97



L494Y
0.95



L469V
0.95



L494G
0.94



L469F
0.93



L494I
0.93



L469I
0.92



A453T
0.91



A453A
0.90



L494V
0.90



L494R
0.89



A453W
0.88



L469N
0.88



A453C
0.88



L469T
0.88



L469S
0.87



L469H
0.87



N454N
0.86



L494C
0.86



L494D
0.86



L494E
0.86



L469C
0.85



A453S
0.85



A453A
0.85



N454S
0.84



A453A
0.84



L469P
0.83



L494S
0.83



N454G
0.83



A453N
0.83



L494H
0.83



L469A
0.83



L469L
0.82



L494N
0.81



L469Q
0.79



L494L
0.78



A453E
0.77



L469L
0.77



N454H
0.76



A453L
0.76



A453H
0.75



L469G
0.75



L494Q
0.74



N454A
0.73



L494P
0.73



N454T
0.73



L469L
0.73



N454E
0.72



A453I
0.70



L494A
0.69



N454Y
0.67



N454W
0.66



A453V
0.64



N454V
0.63



A453F
0.62



N454Q
0.62



N454L
0.61



A453R
0.59



N454D
0.56



A453D
0.54



N454C
0.53



N454F
0.49



A453K
0.45



L494T
0.42



N454I
0.31



A453G
0.26



N454M
0.22



L469Y
0.19



A453P
0.02



N454P
0.00



A453Y
ND



N454R
−0.03







Growth Index Ranking for Plate 006.










A519W
1.27



E525K
1.13



H515Y
1.07



R528K
1.05



E525E
1.01



A519K
0.99



H515K
0.98



H515W
0.97



H515R
0.96



E525W
0.94



R528R
0.92



R528R
0.92



A519A
0.91



E525L
0.89



A519R
0.89



A519A
0.87



R528V
0.85



A519A
0.85



E525V
0.85



A519G
0.85



H515G
0.85



A519A
0.85



R528R
0.85



E525R
0.83



E525E
0.83



E525D
0.83



E525T
0.82



E525C
0.81



E525G
0.80



E525M
0.79



H515F
0.78



H515M
0.78



H515V
0.78



E525F
0.77



A519H
0.76



A519F
0.76



H515Q
0.73



A519Y
0.73



H515T
0.73



E525H
0.73



E525N
0.73



E525Q
0.72



R528M
0.70



H515N
0.70



E525A
0.69



R528F
0.69



E525E
0.67



R528H
0.67



A519S
0.66



A519L
0.66



H515E
0.66



E525S
0.64



H515S
0.63



A519T
0.61



R528Y
0.61



R528T
0.60



R528L
0.59



H515A
0.58



A519C
0.50



R528A
0.49



A519V
0.45



A519Q
0.44



R528S
0.43



A519E
0.39



E525P
0.37



R528N
0.35



R528E
0.34



A519D
0.33



R528G
0.33



R528C
0.31



R528W
0.30



H515L
0.15



R528D
0.08



A519P
0.00



H515H
−0.01



H515H
ND



H515P
−0.02



R528P
−0.02



H515H
ND



H515H
ND







Growth Index Ranking for Plate 007.










T536L
0.62



T536T
0.59



T536Y
0.58



T536I
0.49



T536H
0.46



T536F
0.45



T536G
0.42



T536T
0.40



T536V
0.38



T536S
0.36



T536K
0.31



T536T
0.30



T536M
0.29



T536N
0.29



T536C
0.27



T536A
0.26



T536D
0.18



T536R
0.15



T536E
0.14



T536P
0.01







Growth Index Ranking for Plate 008.










G78L
1.33



G78Y
1.31



G78W
1.27



G78I
1.26



S74K
1.24



K36L
1.23



S74W
1.22



S74I
1.20



K36K
1.20



K36I
1.19



G78V
1.19



K36V
1.19



G78H
1.18



G78T
1.18



S74V
1.17



K36Y
1.17



S74L
1.14



S74T
1.14



S74Y
1.13



G78K
1.13



K36R
1.11



S74H
1.10



K36T
1.07



G78F
1.01



K36W
1.01



S74G
0.98



K36S
0.98



G78R
0.97



S74R
0.97



K36H
0.97



K36F
0.95



S74S
0.94



G78G
0.91



G78S
0.91



S74F
0.86



G78C
0.84



K36G
0.83



S74C
0.78



K36M
0.77



A7G
0.77



S74Q
0.77



G78P
0.76



S74P
0.76



G78E
0.74



S74N
0.72



G78D
0.72



K36N
0.72



S74E
0.72



S74D
0.70



S74A
0.70



G78M
0.70



A7A
0.69



K36C
0.69



G78N
0.68



G78Q
0.68



G78A
0.66



S74M
0.65



K36Q
0.65



K36P
0.61



K36D
0.58



K36A
0.56



K36E
0.54



A7W
0.50



A7V
0.39



A7C
0.34



A7H
0.31



A7P
0.31



A7Y
0.30



A7S
0.30



A7T
0.29



A7I
0.29



A7F
0.20



A7Q
0.19



A7E
0.17



A7N
0.16



A7L
0.09



A7D
0.07



A7R
0.07



A7M
0.06



A7K
−0.02







Growth Index Ranking for Plate 009.










R44H
1.30



R44T
1.27



R44F
1.21



G121I
1.17



R44V
1.17



G121L
1.15



G121H
1.14



R44Y
1.13



R44I
1.10



G121F
1.09



G121T
1.07



G121Y
1.07



R44K
1.04



G121V
1.03



G121K
1.02



G121W
1.00



R44C
1.00



R44A
1.00



Q216H
0.99



R44M
0.99



R44L
0.99



R44D
0.96



R44N
0.96



R44S
0.95



Q216I
0.94



G121A
0.94



Q216T
0.92



G121G
0.91



Q216V
0.90



G121M
0.90



Q216K
0.89



G121C
0.88



E488I
0.88



Q216F
0.87



Q216L
0.87



G121R
0.87



Q216W
0.86



Q216Y
0.84



Q216A
0.83



G121P
0.83



R44E
0.82



Q216C
0.81



R44Q
0.80



R44W
0.79



R44P
0.78



Q216G
0.78



R44R
0.78



E488A
0.77



E488L
0.76



G121D
0.75



Q216E
0.75



G121S
0.74



Q216S
0.74



G121N
0.74



G121Q
0.72



E488V
0.72



Q216M
0.72



Q216N
0.71



G121E
0.71



E488W
0.70



Q216D
0.70



Q216Q
0.68



E488F
0.67



E488C
0.66



E488E
0.66



E488G
0.66



E488D
0.65



E488T
0.65



E488H
0.64



E488Q
0.61



Q216P
0.60



Q216R
0.60



E488R
0.54



R44G
0.53



E488M
0.52



E488Y
0.44



E488S
0.43



E488K
0.32



E488N
0.25



E488P
0.17







Growth Index Ranking for Plate 010.










E179L
1.46



E179I
1.40



T251T
1.36



E179K
1.35



E179H
1.33



E179W
1.26



G177V
1.26



G177T
1.25



E179T
1.23



G177L
1.22



E179V
1.22



G177I
1.18



R246R
1.17



E179Y
1.14



G177H
1.14



G177K
1.13



R246K
1.13



E179F
1.11



G177P
1.11



T251N
1.10



T251Y
1.09



E179S
1.07



E179G
1.05



T251H
1.04



R246T
1.04



G177Y
1.03



R246H
1.03



G177A
1.03



T251K
1.01



G177M
1.01



E179M
1.01



G177W
1.00



T251S
1.00



E179A
0.96



T251R
0.94



E179R
0.94



E179C
0.94



R246G
0.94



G177G
0.93



G177N
0.93



T251G
0.92



G177S
0.92



T251Q
0.91



G177R
0.90



R246S
0.90



G177C
0.88



R246N
0.87



R246Q
0.85



E179D
0.84



E179P
0.82



G177F
0.81



R246E
0.80



E179N
0.79



T251E
0.79



R246D
0.79



G177D
0.79



T251D
0.78



T251C
0.77



T251M
0.76



R246A
0.75



E179Q
0.74



G177E
0.74



T251A
0.74



T251W
0.73



T251V
0.71



T251F
0.71



T251L
0.69



R246M
0.65



R246Y
0.61



R246C
0.61



R246L
0.54



R246V
0.53



R246W
0.53



T251I
0.52



G177Q
0.44



R246I
0.40



T251P
0.37



R246F
0.30



R246P
0.01



E179E
0.00







Growth Index Ranking for Plate 011.










H254H
1.32



H254K
1.20



L308H
1.19



L308I
1.18



H254F
1.17



H254V
1.16



H254W
1.15



H254I
1.12



H254T
1.11



H254R
1.07



L308W
1.07



L308L
1.05



H254Y
0.97



L308R
0.94



V290I
0.92



L308Y
0.92



V290V
0.89



H254G
0.89



H254L
0.88



H254S
0.87



L308C
0.85



H254D
0.85



L308G
0.84



H254E
0.81



H254A
0.80



H254C
0.78



L308D
0.76



F287F
0.74



L308N
0.71



L308E
0.71



L308S
0.69



H254M
0.68



H254Q
0.68



L308Q
0.65



L308V
0.59



H254N
0.59



V290T
0.59



V290L
0.48



F287W
0.42



F287L
0.39



F287M
0.26



F287V
0.21



L308P
0.20



L308A
0.19



F287Y
0.19



V290C
0.18



L308T
0.17



V290A
0.17



V290S
0.16



V290G
0.15



L308K
0.15



F287A
0.10



F287H
0.09



F287K
0.08



L308F
0.06



V290H
0.04



F287T
0.04



V290K
0.04



F287Q
0.03



V290W
0.03



F287S
0.02



V290Y
0.02



L308M
0.02



F287G
0.02



F287I
0.01



F287N
0.01



F287R
0.01



V290R
0.00



H254P
ND



V290F
0.00



F287C
0.00



F287P
−0.01



V290M
−0.01



V290Q
−0.02



F287D
−0.02



V290E
−0.02



F287E
−0.02



V290N
−0.03



V290D
−0.03



V290P
−0.03







Growth Index Ranking for Plate 012.










Q421R
1.39



Q421H
1.26



Q421E
1.24



T426I
1.19



Q421G
1.16



Q421V
1.13



Q421K
1.12



P430S
1.10



P430T
1.10



Q421I
1.09



Q421T
1.08



Q421Q
1.08



P430V
1.06



T426Y
1.05



Q421W
1.05



Q421L
1.05



Q421M
0.92



Q421A
0.91



Q421Y
0.91



T426S
0.86



T426G
0.86



Q421D
0.86



T426H
0.86



Q421S
0.83



T426F
0.82



T426L
0.82



P430A
0.78



Q421N
0.77



T426C
0.76



Q421P
0.74



T426W
0.73



P430C
0.72



T426A
0.68



T426M
0.66



T426D
0.66



T426N
0.60



T426A
0.59



T426E
0.59



F434I
0.55



F434L
0.48



T426Y
0.48



T426P
0.46



F434V
0.46



F434M
0.45



F434T
0.44



T426K
0.40



T426R
0.38



P430G
0.30



F434F
0.24



F434C
0.23



F434A
0.17



F434Y
0.14



F434S
0.10



F434H
0.09



F434K
0.05



F434N
0.05



T426T
0.04



F434Q
0.04



F434G
0.03



P430H
0.02



P430I
0.02



P430W
0.02



F434P
0.01



P430K
0.01



P430Q
0.01



Q421F
0.01



F434E
0.01



F434D
0.01



F434W
0.01



Q421C
0.00



P430R
0.00



F434R
0.00



P430M
0.00



P430D
0.00



P430E
0.00



P430F
0.00



P430P
0.00



P430L
0.00



P430Y
0.00



P430N
−0.01







Growth Index Ranking for Plate 013.










A445H
1.18



F403V
1.06



G397G
1.05



F403T
1.03



F403I
0.93



A445S
0.92



F403Y
0.91



F403L
0.91



A445K
0.86



F403S
0.81



F403H
0.78



F403F
0.76



A445Q
0.74



Q400Q
0.74



F403A
0.72



F403G
0.71



F403M
0.70



A445T
0.70



A445R
0.68



A445A
0.68



F403C
0.67



A445E
0.67



A445C
0.65



A445N
0.64



A445G
0.62



A445D
0.62



A445F
0.61



Q400L
0.61



A445M
0.60



F403N
0.58



A445L
0.53



A445Y
0.53



A445P
0.52



Q400H
0.52



A445Y
0.51



Q400T
0.51



Q400C
0.46



A445W
0.44



Q400N
0.43



Q400M
0.43



A445I
0.42



G397A
0.40



F403Q
0.38



Q400S
0.27



Q400V
0.20



F403E
0.18



G397V
0.16



G397I
0.09



Q400G
0.09



G397M
0.08



F403W
0.05



G397C
0.05



Q400E
0.02



Q400A
0.01



F403D
0.01



Q400P
0.01



Q400I
0.00



G397Q
0.00



G397R
0.00



G397P
0.00



G397S
0.00



G397Y
0.00



G397N
0.00



F403P
−0.01



F403K
−0.01



G397L
−0.01



Q400R
−0.01



Q400F
−0.01



Q400K
−0.02



G397E
−0.02



Q400D
−0.02



G397F
−0.02



G397H
−0.02



F403R
−0.02



Q400Y
−0.02



G397D
−0.02



Q400W
−0.03



G397K
−0.03



G397W
−0.03



G397T
−0.03







Growth Index Ranking for Plate 014.










D33T
1.49



D33V
1.46



F53L
1.44



G99T
1.43



D33Y
1.39



K50K
1.35



F53T
1.34



K50I
1.33



K50L
1.33



G99V
1.32



D33W
1.30



F53W
1.27



G99I
1.27



F53V
1.26



F53I
1.26



D33H
1.26



G99K
1.24



F53Y
1.24



D33I
1.23



G99Y
1.23



G99L
1.23



D33S
1.22



K50W
1.22



D33K
1.21



D33R
1.18



K50H
1.18



K50Y
1.17



F53R
1.17



K50T
1.14



D33L
1.13



F53S
1.12



K50V
1.11



G99S
1.11



G99R
1.09



F53K
1.08



D33Q
1.07



D33F
1.07



F53F
1.06



F53H
1.05



F53G
1.05



G99W
1.03



G99F
1.02



D33G
0.97



F53Q
0.96



G99Q
0.94



K50S
0.91



F53E
0.90



F53P
0.87



D33E
0.83



K50E
0.83



D33P
0.82



D33D
0.81



G99P
0.80



G99E
0.78



K50G
0.77



K50Q
0.74



F53D
0.71



K50P
0.70



G99D
0.70



K50D
0.68



D33N
0.64



G99N
0.57



D33C
0.50



G99C
0.46



F53C
0.44



F53N
0.43



K50C
0.43



G99M
0.43



K50N
0.35



G99H
ND



K50A
0.27



G99A
0.22



F53A
0.16



K50M
0.11



D33A
0.04



K50R
ND



G99G
ND



K50F
ND



D33M
−0.03



F53M
−0.07







Growth Index Ranking for Plate 015.










D23H
1.04



D23V
0.96



D23T
0.94



E26G
0.93



D81T
0.93



D23F
0.92



D23S
0.91



D23I
0.89



D23K
0.88



D23W
0.88



D23E
0.86



D81V
0.85



D81H
0.85



D81L
0.84



D81F
0.83



D23G
0.82



D23L
0.82



D23D
0.82



D23R
0.81



D81A
0.81



D23C
0.81



D81S
0.80



S27H
0.80



D81Y
0.80



D81G
0.80



S27L
0.78



D23P
0.78



D23M
0.77



D23N
0.76



D23Y
0.75



S27Q
0.73



D81C
0.71



D81M
0.70



D81N
0.69



D81I
0.69



E26H
0.68



S27K
0.67



S27I
0.67



D23Q
0.66



E26I
0.65



E26V
0.65



E26K
0.64



S27T
0.64



D81Q
0.63



S27M
0.63



D81W
0.62



D81R
0.62



D81K
0.61



S27G
0.59



S27C
0.59



S27V
0.58



E26L
0.58



E26T
0.55



E26Q
0.55



E26E
0.54



S27N
0.54



S27S
0.54



S27R
0.54



D23A
0.53



E26S
0.52



S27E
0.51



D81E
0.51



S27A
0.49



E26N
0.49



E26P
0.49



E26D
0.47



E26M
0.46



S27F
0.46



E26R
0.46



E26C
0.45



S27D
0.44



D81P
0.42



S27P
0.41



S27Y
0.38



E26W
0.34



E26F
0.33



S27W
0.30



E26Y
0.29



E26A
0.24



D81D
0.01







Growth Index Ranking for Plate 016.










G69S
1.21



G69M
1.16



L20M
1.14



G69G
1.13



G69K
1.12



G69T
1.11



G69I
1.10



G69L
1.10



Y16M
1.09



Y16I
1.09



L20V
1.08



G69V
1.08



L20S
1.08



G69H
1.08



G69R
1.06



G69Q
1.05



Y16L
1.05



G69N
1.04



Y18G
1.03



L20Y
1.03



G69A
1.03



L20L
1.03



Y16Y
1.01



L20T
1.01



Y18C
1.00



L20W
1.00



G69E
1.00



G69C
0.99



Y18H
0.97



L20F
0.97



L20I
0.96



G69F
0.96



Y18N
0.96



Y18Y
0.95



L20A
0.95



G69Y
0.94



G69W
0.92



Y18I
0.91



Y18W
0.91



Y16V
0.91



Y16H
0.86



Y16F
0.85



G69D
0.84



Y18R
0.83



Y18F
0.82



Y18T
0.80



Y18V
0.78



Y16T
0.77



L20H
0.77



Y18M
0.76



L20N
0.76



Y18L
0.75



L20G
0.75



L20Q
0.75



Y18Q
0.74



L20C
0.74



Y16W
0.73



Y18S
0.72



G69P
0.72



Y18D
0.67



L20E
0.66



Y18K
0.65



Y18E
0.61



Y16E
0.53



L20R
0.53



Y16C
0.50



Y18P
0.49



Y16Q
0.48



Y16P
0.48



Y18A
0.45



L20D
0.45



Y16S
0.44



Y16N
0.41



Y16D
0.40



Y16K
0.39



L20K
0.36



L20P
0.36



Y16G
0.36



Y16A
0.34



Y16R
0.21







Growth Index Ranking for Plate 017.










A3T
1.39



A3H
1.21



A3K
1.13



A3F
1.10



A3Y
1.09



A3I
1.05



S13L
1.04



A3R
1.04



A3Q
0.97



A3E
0.94



S13T
0.90



A3N
0.89



S13H
0.87



S13K
0.85



Y9F
0.84



N12S
0.83



A3L
0.83



S13I
0.82



S13V
0.81



A3D
0.79



N12T
0.79



A3G
0.78



S13R
0.76



S13G
0.76



A3P
0.76



N12C
0.73



A3A
0.73



S13Y
0.72



N12A
0.72



A3W
0.70



S13M
0.69



S13S
0.68



A3C
0.68



N12N
0.68



S13N
0.67



S13F
0.67



S13W
0.64



S13Q
0.62



N12M
0.61



N12V
0.59



S13A
0.57



Y9W
0.56



S13C
0.56



A3S
0.51



S13E
0.50



N12H
0.48



N12I
0.44



N12P
0.43



N12R
0.41



N12Q
0.38



S13D
0.35



N12G
0.33



Y9H
0.31



N12K
0.29



N12D
0.27



N12L
0.25



N12F
0.25



Y9I
0.22



Y9V
0.20



S13P
0.18



A3M
0.16



Y9T
0.15



N12E
0.15



A3V
0.14



N12W
0.14



Y9R
0.11



Y9P
0.10



Y9L
0.09



Y9A
0.09



Y9S
0.08



Y9C
0.05



Y9N
0.04



Y9Y
0.04



Y9K
0.03



Y9Q
0.03



Y9M
0.02



Y9G
0.01



Y9D
−0.01



Y9E
−0.01



N12Y
−0.01







Growth Index Ranking for Plate 018.










A139T
1.29



Q197T
1.19



G127T
1.19



R194H
1.16



Q197V
1.15



G127H
1.13



G127F
1.08



A139P
1.07



A139H
1.07



A139S
1.07



A139V
1.07



G127V
1.05



Q197H
1.04



A139I
1.04



Q197I
1.04



A139C
1.03



G127I
1.03



G127L
1.03



Q197M
1.01



G127Y
1.01



Q197F
0.99



A139Q
0.98



A139G
0.97



Q197Y
0.97



Q197S
0.97



G127S
0.96



Q197E
0.95



A139E
0.95



R194R
0.93



R194Y
0.92



Q197L
0.91



Q197N
0.90



Q197K
0.90



Q197D
0.89



A139L
0.88



G127E
0.88



A139D
0.87



G127P
0.87



G127C
0.87



R194L
0.87



Q197C
0.87



G127W
0.86



Q197R
0.86



G127D
0.85



A139M
0.84



R194W
0.84



Q197G
0.83



Q197A
0.83



A139A
0.83



A139N
0.83



Q197W
0.83



R194F
0.82



Q197P
0.82



A139F
0.82



R194K
0.81



G127M
0.80



A139W
0.80



Q197Q
0.75



G127N
0.74



R194C
0.72



G127Q
0.71



G127A
0.70



R194M
0.68



R194I
0.65



R194Q
0.61



G127R
0.59



G127K
0.58



A139Y
0.56



R194V
0.43



A139R
0.30



A139K
0.30



R194A
0.27



R194N
0.18



R194T
0.15



R194S
0.08



R194E
0.03



R194D
0.02



G127G
0.00



R194P
−0.01



R194G
−0.03







Growth Index Ranking for Plate 019.










L59H
1.96



L59K
1.49



Q116V
1.47



L59I
1.46



E117I
1.42



L59R
1.41



Q116W
1.36



Q116T
1.36



Q116Y
1.32



E117L
1.29



L59G
1.28



E117W
1.27



L59F
1.26



L59A
1.25



Q116I
1.22



L59M
1.22



E117F
1.21



L59C
1.21



Q116F
1.20



L59E
1.19



L59T
1.18



L59D
1.12



Q116L
1.12



Q116K
1.09



L59L
1.09



E117H
1.08



Q125M
1.06



L59W
1.06



Q125I
1.05



L59N
1.05



Q125W
1.05



L59V
1.05



L59Q
1.04



Q125H
1.02



Q116S
1.02



Q125Y
1.01



Q125T
1.01



Q116A
1.01



E117M
1.00



Q116C
0.99



Q125P
0.99



E117V
0.99



L59Y
0.97



Q125N
0.97



Q116D
0.97



Q125V
0.96



E117A
0.95



E117Y
0.94



Q125A
0.93



Q125L
0.93



Q116E
0.92



Q116H
0.92



Q125F
0.92



Q116P
0.91



Q116R
0.91



E117C
0.90



Q125C
0.90



Q125R
0.89



Q125K
0.89



Q116G
0.86



E117T
0.86



Q125S
0.85



Q125D
0.83



E117N
0.82



Q125G
0.82



Q116Q
0.81



Q116N
0.80



E117R
0.80



E117S
0.80



Q116M
0.76



L59S
0.74



Q125E
0.73



E117G
0.70



E117D
0.70



Q125Q
0.69



E117Q
0.66



E117K
0.55



E117P
0.44



E117E
0.26



L59P
0.03







Growth Index Ranking for Plate 020.










Y377W
1.26



G389K
1.24



L376L
1.24



Y377Y
1.23



L376I
1.20



G389T
1.18



Y377V
1.17



G389L
1.16



G389H
1.13



G389V
1.12



K379R
1.12



Y377H
1.12



Y377L
1.10



K379V
1.10



L376Y
1.09



K379T
1.09



G389R
1.06



K379H
1.04



G389I
1.03



Y377I
1.03



G389Y
1.01



G389M
1.01



G389S
1.01



Y377T
0.98



G389N
0.96



Y377F
0.95



K379L
0.95



G389G
0.94



K379G
0.93



L376F
0.92



K379W
0.92



G389F
0.88



K379S
0.88



G389W
0.87



K379I
0.86



K379A
0.86



K379P
0.83



G389C
0.83



G389P
0.82



K379Q
0.81



G389Q
0.80



K379N
0.79



K379C
0.78



K379M
0.77



K379E
0.77



Y377S
0.77



G389E
0.77



G389A
0.75



G389D
0.73



K379D
0.70



Y377G
0.69



K379F
0.69



L376M
0.65



Y377P
0.61



Y377K
0.60



L376V
0.60



Y377M
0.59



Y377D
0.58



L376W
0.57



Y377N
0.57



Y377E
0.56



Y377C
0.55



Y377A
0.54



Y377Q
0.49



L376H
0.49



L376T
0.48



L376Q
0.48



L376C
0.43



L376A
0.23



Y377R
0.23



L376K
0.22



L376S
0.17



L376N
0.16



L376G
0.14



L376R
0.12



L376P
0.11



L376D
0.08



L376E
0.08



K379Y
0.00



K379K
−0.01







Growth Index Ranking for Plate 021.










I165Y
1.46



E173H
1.25



V202H
1.24



I165H
1.24



E173W
1.23



E174I
1.23



E173T
1.23



E173V
1.20



E174H
1.20



E173L
1.19



E173I
1.19



V202T
1.18



E173Y
1.18



V202R
1.16



E174L
1.16



I165K
1.16



E174V
1.15



V202I
1.15



E174T
1.14



I165T
1.14



E173F
1.14



E174K
1.13



E173K
1.12



I165F
1.11



E174F
1.11



V202K
1.09



E173R
1.08



E173G
1.08



E174R
1.07



E174W
1.05



I165R
1.05



I165I
1.04



I165D
1.03



I165A
1.02



V202F
1.02



V202Y
1.02



E173M
1.01



E173S
1.01



I165L
1.00



E174Y
1.00



I165G
0.99



V202W
0.98



I165W
0.98



I165S
0.95



I165N
0.95



E174M
0.94



E174A
0.94



E173A
0.94



V202C
0.93



V202L
0.93



V202M
0.93



V202A
0.92



I165M
0.91



E173Q
0.90



I165Q
0.90



V202Q
0.90



E174G
0.90



E173N
0.88



V202N
0.88



E174S
0.87



E174N
0.86



E173P
0.86



V202S
0.85



V202E
0.85



V202D
0.84



E173C
0.83



E174C
0.83



I165E
0.83



E173E
0.81



V202G
0.81



I165C
0.79



E174Q
0.72



E174D
0.70



E174E
0.69



E174P
0.68



I165P
0.67



E173D
0.65



I165V
0.61



V202P
0.35



V202V
0.32







Growth Index Ranking for Plate 022.










K489K
1.65



G491I
1.57



L490I
1.52



L490H
1.46



G491H
1.41



L490V
1.41



I539V
1.40



G491V
1.36



G491L
1.36



K489W
1.35



L490W
1.34



G491W
1.33



G491T
1.33



G491Y
1.31



G491K
1.29



I539T
1.26



L490L
1.25



K489R
1.24



L490T
1.21



K489I
1.21



I539L
1.21



K489T
1.20



I539I
1.17



G491R
1.16



G491M
1.14



K489Y
1.14



L490F
1.14



G491F
1.13



K489H
1.08



G491A
1.08



G491C
1.05



K489L
1.05



L490M
1.04



G491S
1.03



I539K
1.00



G491N
1.00



L490S
0.98



G491D
0.96



I539M
0.96



L490N
0.95



K489A
0.94



L490K
0.94



G491E
0.94



G491Q
0.93



K489F
0.93



G491G
0.91



L490R
0.90



L490A
0.90



I539S
0.90



I539H
0.89



I539C
0.88



L490C
0.88



K489S
0.88



L490G
0.86



K489G
0.84



K489Y
0.83



L490D
0.78



I539A
0.77



L490E
0.75



K489C
0.68



I539Y
0.67



K489Q
0.66



I539F
0.65



I539R
0.64



I539W
0.64



I539Q
0.64



L490Q
0.62



K489D
0.62



K489N
0.61



K489E
0.58



I539P
0.53



K489P
0.50



I539E
0.36



I539N
0.32



I539G
0.28



K489M
0.23



G491P
0.21



L490P
0.17



I539D
−0.01



L490Y
−0.01







Growth Index Ranking for Plate 023.










Q509T
1.49



K487T
1.39



Q509V
1.34



Q509I
1.30



K487H
1.19



K487K
1.18



K487V
1.12



K487L
1.11



Y514Y
1.08



K487C
1.06



K487W
1.05



Q509S
1.03



K487R
1.03



K487F
1.03



K487A
0.99



K487G
0.97



K487S
0.93



Q509G
0.91



Q509Q
0.90



K487E
0.90



Q509C
0.90



K487I
0.90



K487M
0.87



Q509A
0.86



K487A
0.81



Q509M
0.80



T521G
0.77



T521E
0.77



Q509N
0.74



T521S
0.73



K487D
0.69



K487Y
0.61



T521L
0.60



Q509H
0.53



T521Q
0.51



K487N
0.44



Q509E
0.40



T521V
0.36



Q509D
0.28



Q509K
0.26



Q509L
0.16



K487P
0.15



T521M
0.13



T521H
0.02



Y514H
0.01



Y514T
0.01



Q509F
0.01



Y514V
0.00



T521T
0.00



Q509Y
0.00



T521A
−0.01



Y514I
−0.01



Y514W
−0.01



T521N
−0.01



Q509R
−0.01



Y514D
−0.02



Y514S
−0.02



T521F
−0.02



T521C
−0.02



Q509P
−0.02



T521Y
−0.02



Y514P
−0.02



T521K
−0.02



Y514R
−0.02



Y514A
−0.02



Y514L
−0.02



Y514N
−0.03



T521I
−0.03



T521R
−0.03



Q509W
−0.03



Y514Q
−0.03



T521P
−0.03



Y514E
−0.03



Y514K
−0.03



Y514C
−0.03



T521W
−0.03



T521D
−0.03



Y514F
−0.04



Y514G
−0.04



Y514M
−0.04







Growth Index Ranking for Plate 024.










L540V
1.73



L540T
1.58



T462T
1.43



A448T
1.35



L540H
1.34



R544T
1.33



T462V
1.31



L540I
1.31



R544W
1.28



L540K
1.28



A448H
1.26



T462K
1.26



T462H
1.26



R544V
1.26



R544S
1.23



A448Y
1.23



L540Y
1.20



R544K
1.19



T462I
1.18



L540W
1.18



A448R
1.15



T462W
1.14



T462Y
1.14



L540R
1.14



L540S
1.12



L540L
1.12



R544H
1.11



A448S
1.11



L540Q
1.09



L540M
1.09



T462F
1.08



T462S
1.06



L540G
1.05



R544I
1.05



R544C
1.04



T462L
1.04



L540F
1.03



R544L
1.02



T462G
1.02



A448I
1.01



L540E
1.01



L540A
1.00



R544M
0.98



L540N
0.98



L540P
0.96



R544F
0.95



R544P
0.95



T462R
0.94



L540D
0.93



T462A
0.93



R544G
0.91



L540C
0.90



T462M
0.89



A448K
0.89



R544N
0.89



A448Y
0.88



A448G
0.87



R544R
0.86



R544Q
0.86



A448L
0.85



T462C
0.84



R544E
0.84



T462D
0.84



R544A
0.83



R544D
0.83



T462Q
0.83



A448C
0.83



A448Q
0.82



T462N
0.81



A448N
0.79



A448M
0.78



A448F
0.78



A448A
0.77



A448D
0.74



A448W
0.72



A448E
0.72



A448P
0.70



T462P
0.60



R544Y
0.50



T462E
−0.08







Growth Index Ranking for Plate 025.










P498H
1.26



S457S
1.23



A496H
1.20



A496T
1.17



S457H
1.16



P498T
1.16



A496I
1.14



K497T
1.13



K497V
1.12



P498R
1.12



K497K
1.12



P498I
1.11



P498K
1.11



P498V
1.10



S457R
1.10



P498Y
1.10



P498L
1.09



S457T
1.09



A496L
1.06



A496R
1.06



S457Q
1.06



A496V
1.04



K497H
1.04



P498S
1.03



A496K
1.03



K497L
1.03



K497I
1.02



P498F
1.02



A496Y
1.01



P498G
1.01



K497Y
0.99



S457Y
0.99



A496W
0.98



A496F
0.97



K497G
0.96



K497F
0.94



A496S
0.93



K497S
0.92



P498W
0.90



P498P
0.88



P498M
0.86



P498D
0.86



P498E
0.86



K497R
0.85



K497A
0.84



A496M
0.84



S457K
0.84



K497W
0.82



A496G
0.82



A496P
0.82



S457N
0.82



K497D
0.81



P498Q
0.81



S457F
0.80



P498N
0.80



A496E
0.80



A496A
0.76



A496Q
0.76



S457M
0.76



S457D
0.75



K497Q
0.75



P498C
0.75



A496D
0.74



S457W
0.74



S457E
0.74



K497M
0.74



K497N
0.73



K497E
0.72



A496C
0.70



K497P
0.69



A496N
0.66



S457G
0.65



P498A
0.64



K497C
0.64



S457L
0.62



S457V
0.59



S457C
0.47



S457I
0.43



S457A
0.33



S457P
0.14







Growth Index Ranking for Plate 026.










G87K
1.34



S120L
1.34



G87T
1.24



G87L
1.24



S120I
1.20



S120K
1.18



G87V
1.17



G87Y
1.17



S120T
1.16



T240T
1.15



S120W
1.14



G87H
1.12



G87W
1.11



D25H
1.11



G87I
1.10



D25L
1.10



D25I
1.09



S120H
1.08



S120Y
1.07



D25T
1.06



S120V
1.05



G87M
1.04



G87R
1.03



D25V
1.02



S120F
1.01



D25K
1.00



D25Y
1.00



T240L
0.99



G87F
0.99



G87P
0.98



D25P
0.97



S120M
0.96



G87S
0.96



T240I
0.96



T240V
0.95



G87D
0.94



D25N
0.93



D25D
0.93



D25M
0.90



D25A
0.89



G87C
0.89



S120C
0.88



S120E
0.88



G87A
0.88



D25G
0.88



S120D
0.88



D25F
0.88



S120G
0.87



D25W
0.86



S120R
0.84



S120A
0.82



D25E
0.82



G87E
0.81



T240M
0.81



G87N
0.80



D25S
0.80



S120N
0.80



D25R
0.77



T240A
0.76



G87Q
0.75



S120Q
0.74



D25Q
0.73



S120S
0.69



T240N
0.69



T240C
0.67



T240Q
0.67



D25C
0.66



S120P
0.60



T240S
0.56



T240G
0.35



T240H
0.15



T240Y
0.09



T240E
0.08



T240W
0.08



T240F
0.05



T240D
0.03



T240K
0.02



T240P
0.01



T240R
0.01



G87G
−0.01







Growth Index Ranking for Plate 027.










F495L
1.84



F495T
1.56



S493T
1.53



G492A
1.48



F495Y
1.45



F495K
1.45



F495W
1.44



S493V
1.40



F495S
1.40



G492T
1.38



F495V
1.38



G492H
1.38



G492V
1.37



S493R
1.35



F495H
1.31



G492K
1.30



S493W
1.29



F495R
1.28



S493E
1.27



G492I
1.26



S493L
1.26



F495M
1.25



G492E
1.25



G492D
1.24



N476Y
1.24



S493K
1.23



S493G
1.21



S493I
1.20



S493S
1.20



G492L
1.18



G492W
1.18



F495Q
1.16



F495I
1.15



N476W
1.15



N476V
1.14



N476R
1.13



G492G
1.11



G492C
1.11



N476T
1.09



S493M
1.09



S493Y
1.08



F495F
1.07



G492Y
1.07



G492R
1.06



S493C
1.04



S493A
1.03



F495G
1.02



F495A
1.02



G492N
0.99



F495N
0.98



F495D
0.96



S493H
0.95



F495C
0.95



F495P
0.95



G492F
0.94



F495E
0.94



G492Q
0.93



S493P
0.93



G492M
0.89



G492P
0.89



G492S
0.86



N476Q
0.83



S493Q
0.83



N476S
0.82



S493F
0.81



S493N
0.79



N476D
0.73



N476M
0.71



N476P
0.64



N476E
0.62



N476A
0.02



N476N
0.01



S493D
0.00



N476I
−0.01



N476H
−0.01



N476G
−0.02



N476F
−0.04



N476C
−0.04



N476K
−0.05



N476L
−0.08










Results/Discussion

Table 6 lists all variants identified that displayed growth indices of 1.2 or higher. Without being bound by theory, mutations at these positions may result in increased intracellular activity of IspS by several different means. Without being bound by theory, increased intracellular activity could be a result of one or a combination of any of the following properties of IspS: increased cellular viability, increased kcat, decreased Km, increased specific activity, increased solubility, decreased insolubility, improved ribosome binding, increased translation initiation rate, increased translation elongation rate, increased transcription initiation rate, increased transcription elongation rate, decreased secondary structure of DNA, decreased secondary structure of RNA, increased secondary structure of DNA, increased secondary structure of RNA, increased folding rates, increased affinity for intracellular chaperones, increased stability, decreased protein turnover, decreased exposure to intracellular protease, decreased affinity for intracellular protease, decreased localization to the periplasm, improved localization to the cytoplasm, decreased inclusion body formation, decreased membrane localization, increased expression due to a more favorable codon, increased DNA stability, increased RNA stability, and decreased RNA degradation.
Without being bound by theory, any mutation that has a positive effect on the properties of nucleic acid sequences (DNA and RNA) encoding or expressing IspS, or the biochemical properties of the IspS enzyme itself, could allow for greater activity within the cell. All variants with a growth index of 1.2 or higher are subjected to secondary growth assays in a matrix of mevalonic acid and IPTG. These variants are also pooled together and subjected to several rounds of enrichment under IPTG induction and mevalonic acid pathway flux to determine which enzymes allow for the best growth in competition experiments. The most promising variants are examined further for benefits to specific productivity in isoprene producing strains.
Some variants of interest include, but are not limited to, A3T, S13L, I165Y, Q421R, F495L, Q509T, and L540V. All of these variants displayed growth advantages over wild type in secondary assays utilizing IPTG/mevalonic acid matrices. The A3T variant is of particular interest because threonine in this position is found in homologous isoprene synthase enzymes from the related species P. nigra, P. tremuloides and P. trichocharpa. Alteration to a consensus sequence for IspS from Poplar species could allow for a more active enzyme.
The F495L variant is of particular interest because of its location in an exposed surface loop that spans residues K487 to K497. FIG. 5 shows that this variant is proximal to both G491S and L494P, which display positive effects on cell viability in vivo, and specific activity of IspS in vitro, respectively. Additionally, there are several other variants which displayed high growth indices in this surface loop. Together, the benefit of the variants in this loop may indicate that it plays a critical role in enzyme activity within the cell. The Q509T variant is of particular interest because both Q509I and Q509V variants also displayed growth benefits, and these are the only amino acid residues that are branched at the beta carbon. FIG. 6 shows that Q509 is located near but not within the active site of IspS. The presence of a branched structure at the beta carbon of this particular amino acid may confer a benefit to enzyme activity.
Residues listed in Table 7 are necessary for growth under high DMAPP pressure and thus, can be considered in one embodiment as immutable residues. Substitution of the wild type amino acid with any other residue results in minimal to no growth under the growth rate assay conditions. Growth index values for each position are shown in Table 7. Phenylalanine 287 (F287) is located in the active site, and defines the bottom of the active site cavity (FIG. 7). Based on structural alignments with other terpene synthases, F287 determines the length of the substrate that can be accommodated into the active site, thereby preventing access to the active site by isoprenoids with more than five carbons.
Glycine 397 (G397) is located on the side of the active site cavity (FIG. 8). This residue occurs at a kink in an alpha-helix, suggesting that the conformational flexibility of glycine (and other small amino acids) may be required at this position to allow the helix to bend. The bend in the helix is adjacent to the putative substrate binding position in the active site.
Asparagine 438 (N438) is positioned at the top of the active site (FIG. 9). Structural alignments with other terpene synthases indicate that N428 may be involved directly in coordination of the magnesium ions, as well as having possible interactions with the substrate.
Glutamate 451 (E451) is on a substrate access loop located above the active site (FIG. 10). Based on homology modeling and structure-based alignments with other terpene synthases, these loops may have an open position for substrate capture, and then close over the active site once substrate is bound. Residue E451 is proposed to have a role in coordinating one or more magnesium ions during this process.
Tyrosine 514 (Y514) is in the active site, below N438 (FIG. 11). Y514 may be involved in substrate binding, or it may play a direct role in catalysis.







TABLE 6







Variants with Growth Index value equal to or greater than 1.2.










Variant
GI














L59H
1.96



F495L
1.84



L540V
1.73



V30L
1.61



L540T
1.58



G491I
1.57



F495T
1.56



S493T
1.53



V30K
1.52



L490I
1.52



D33T
1.49



Q509T
1.49



L59K
1.49



G492A
1.48



Q116V
1.47



L59I
1.46



E179L
1.46



L490H
1.46



I28T
1.46



D33V
1.46



I165Y
1.46



F495Y
1.45



F495K
1.45



V30Y
1.45



F53L
1.44



F495W
1.44



G99T
1.43



T462T
1.43



E117I
1.42



G491H
1.41



L59R
1.41



L490V
1.41



S493V
1.40



E179I
1.40



I539V
1.40



F495S
1.40



D33Y
1.39



Q421R
1.39



V30W
1.39



A3T
1.39



K487T
1.39



G492T
1.38



F495V
1.38



G492H
1.38



I28S
1.38



G492V
1.37



A443A
1.36



Q116W
1.36



G491V
1.36



Q116T
1.36



G491L
1.36



A448T
1.35



S493R
1.35



K489W
1.35



E179K
1.35



G153W
1.34



L490W
1.34



L540H
1.34



G87K
1.34



S120L
1.34



F53T
1.34



Q509V
1.34



G78L
1.33



K50I
1.33



K50L
1.33



R544T
1.33



E179H
1.33



G491W
1.33



G491T
1.33



Q116Y
1.32



G99V
1.32



F495H
1.31



G491Y
1.31



G78Y
1.31



T462V
1.31



L540I
1.31



I28R
1.31



G492K
1.30



Q509I
1.30



D33W
1.30



R44H
1.30



G491K
1.29



A139T
1.29



L130Y
1.29



E117L
1.29



S493W
1.29



F495R
1.28



R544W
1.28



L540K
1.28



L59G
1.28



A519W
1.27



S493E
1.27



F53W
1.27



E117W
1.27



G99I
1.27



G78W
1.27



R44T
1.27



E179W
1.26



A448H
1.26



F53V
1.26



Y377W
1.26



F53I
1.26



G492I
1.26



T462K
1.26



T462H
1.26



S493L
1.26



G177V
1.26



G78I
1.26



P498H
1.26



L59F
1.26



D33H
1.26



V30S
1.26



Q421H
1.26



I539T
1.26



R544V
1.26



F495M
1.25



E173H
1.25



G492E
1.25



L59A
1.25



G177T
1.25



G87T
1.24



G99K
1.24



V202H
1.24



S74K
1.24



G389K
1.24



G87L
1.24



G492D
1.24



Q421E
1.24



N476Y
1.24



I165H
1.24



F53Y
1.24



K489R
1.24



K36L
1.23



D33I
1.23



R544S
1.23



E179T
1.23



A448V
1.23



G99Y
1.23



E173W
1.23



S493K
1.23



G99L
1.23



E174I
1.23



E173T
1.23



D33S
1.22



G177L
1.22



V30F
1.22



K50W
1.22



Q116I
1.22



E179V
1.22



S74W
1.22



L59M
1.22



D33K
1.21



L490T
1.21



A3H
1.21



K489I
1.21



I539L
1.21



R44F
1.21



E117F
1.21



L59C
1.21



S493G
1.21



G69S
1.21



E173V
1.20



L540Y
1.20



S493I
1.20



S120I
1.20



Q116F
1.20



A496H
1.20



H254K
1.20



S74I
1.20



K489T
1.20



L376I
1.20



E174H
1.20

















TABLE 7







Growth index values for the immutable sites.










Variant
GI











F287










F287A
0.10



F287C
0.00



F287D
−0.02



F287E
−0.02



F287F
0.74



F287G
0.02



F287H
0.09



F287I
0.01



F287K
0.08



F287L
0.39



F287M
0.26



F287N
0.01



F287P
−0.01



F287Q
0.03



F287R
0.01



F287S
0.02



F287T
0.04



F287V
0.21



F287W
0.42



F287Y
0.19







G397










G397A
0.40



G397C
0.05



G397D
−0.02



G397E
−0.02



G397F
−0.02



G397G
1.05



G397H
−0.02



G397I
0.09



G397K
−0.03



G397L
−0.01



G397M
0.08



G397N
0.00



G397P
0.00



G397Q
0.00



G397R
0.00



G397S
0.00



G397T
−0.03



G397V
0.16



G397W
−0.03



G397Y
0.00







N438










N438A
−0.02



N438C
−0.05



N438D
−0.04



N438E
−0.04



N438F
−0.04



N438G
−0.07



N438H
−0.04



N438I
−0.05



N438K
−0.06



N438L
−0.05



N438M
−0.05



N438N
1.27



N438P
−0.06



N438Q
−0.06



N438R
−0.06



N438S
−0.09



N438T
−0.04



N438V
−0.05



N438W
−0.05



N438Y
−0.06







E451










E451A
−0.04



E451C
−0.04



E451D
−0.02



E451E
1.48



E451E
1.13



E451F
−0.03



E451G
−0.02



E451H
−0.02



E451I
−0.03



E451K
−0.01



E451L
−0.05



E451N
−0.06



E451P
−0.05



E451Q
0.06



E451R
−0.03



E451S
−0.06



E451T
−0.04



E451V
−0.04



E451W
−0.01



E451Y
−0.03







Y514










Y514A
−0.02



Y514C
−0.03



Y514D
−0.02



Y514E
−0.03



Y514F
−0.04



Y514G
−0.04



Y514H
0.01



Y514I
−0.01



Y514K
−0.03



Y514L
−0.02



Y514M
−0.04



Y514N
−0.03



Y514P
−0.02



Y514Q
−0.03



Y514R
−0.02



Y514S
−0.02



Y514T
0.01



Y514V
0.00



Y514W
−0.01



Y514Y
1.08










Example 3
Positional Analysis of Growth Index
This Example shows the results of analysis of SEL data displayed is by position. The growth index that corresponds to the particular mutation is shown as below in Table 8:
















TABLE 8






Growth

Growth

Growth

Growth

Growth


Mutation
Index
Mutation
Index
Mutation
Index
Mutation
Index
Mutation
Index
























A3A
0.729
A7A
0.695
Y9A
0.088
N12A
0.719
S13A
0.572


A3C
0.677
A7C
0.337
Y9C
0.048
N12C
0.731
S13C
0.560


A3D
0.792
A7D
0.070
Y9D
−0.009
N12D
0.267
S13D
0.351


A3E
0.936
A7E
0.167
Y9E
−0.011
N12E
0.146
S13E
0.496


A3F
1.103
A7F
0.203
Y9F
0.839
N12F
0.251
S13F
0.667


A3G
0.780
A7G
0.766
Y9G
0.013
N12G
0.329
S13G
0.763


A3H
1.211
A7H
0.309
Y9H
0.314
N12H
0.478
S13H
0.869


A3I
1.051
A7I
0.291
Y9I
0.216
N12I
0.442
S13I
0.823


A3K
1.135
A7K
−0.020
Y9K
0.035
N12K
0.293
S13K
0.853


A3L
0.830
A7L
0.092
Y9L
0.094
N12L
0.252
S13L
1.039


A3M
0.157
A7M
0.063
Y9M
0.016
N12M
0.606
S13M
0.686


A3N
0.889
A7N
0.156
Y9N
0.043
N12N
0.676
S13N
0.672


A3P
0.763
A7P
0.309
Y9P
0.100
N12P
0.428
S13P
0.177


A3Q
0.966
A7Q
0.188
Y9Q
0.028
N12Q
0.378
S13Q
0.616


A3R
1.037
A7R
0.069
Y9R
0.115
N12R
0.406
S13R
0.764


A3S
0.511
A7S
0.301
Y9S
0.079
N12S
0.833
S13S
0.682


A3T
1.391
A7T
0.294
Y9T
0.154
N12T
0.790
S13T
0.903


A3V
0.144
A7V
0.392
Y9V
0.201
N12V
0.588
S13V
0.809


A3W
0.702
A7W
0.501
Y9W
0.561
N12W
0.137
S13W
0.643


A3Y
1.092
A7Y
0.305
Y9Y
0.036
N12Y
−0.012
S13Y
0.724


Y16A
0.337
Y18A
0.453
L20A
0.946
D23A
0.530
D25A
0.889


Y16C
0.497
Y18C
1.005
L20C
0.739
D23C
0.807
D25C
0.659


Y16D
0.402
Y18D
0.669
L20D
0.450
D23D
0.819
D25D
0.926


Y16E
0.533
Y18E
0.610
L20E
0.655
D23E
0.855
D25E
0.822


Y16F
0.846
Y18F
0.825
L20F
0.966
D23F
0.921
D25F
0.878


Y16G
0.356
Y18G
1.031
L20G
0.749
D23G
0.825
D25G
0.878


Y16H
0.863
Y18H
0.973
L20H
0.772
D23H
1.039
D25H
1.111


Y16I
1.088
Y18I
0.913
L20I
0.962
D23I
0.893
D25I
1.087


Y16K
0.394
Y18K
0.652
L20K
0.364
D23K
0.883
D25K
1.003


Y16L
1.049
Y18L
0.750
L20L
1.025
D23L
0.822
D25L
1.096


Y16M
1.092
Y18M
0.762
L20M
1.139
D23M
0.766
D25M
0.900


Y16N
0.409
Y18N
0.955
L20N
0.755
D23N
0.763
D25N
0.929


Y16P
0.479
Y18P
0.486
L20P
0.357
D23P
0.783
D25P
0.965


Y16Q
0.481
Y18Q
0.739
L20Q
0.746
D23Q
0.665
D25Q
0.726


Y16R
0.214
Y18R
0.825
L20R
0.526
D23R
0.813
D25R
0.774


Y16S
0.438
Y18S
0.720
L20S
1.080
D23S
0.910
D25S
0.802


Y16T
0.772
Y18T
0.799
L20T
1.007
D23T
0.939
D25T
1.057


Y16V
0.911
Y18V
0.783
L20V
1.083
D23V
0.957
D25V
1.023


Y16W
0.730
Y18W
0.912
L20W
0.998
D23W
0.879
D25W
0.859


Y16Y
1.014
Y18Y
0.949
L20Y
1.031
D23Y
0.747
D25Y
0.998


E26A
0.244
S27A
0.493
I28A
0.256
V30A
0.256
D33A
0.039


E26C
0.455
S27C
0.593
I28C
0.101
V30C
0.308
D33C
0.496


E26D
0.475
S27D
0.442
I28D
0.383
V30D
−0.014
D33D
0.813


E26E
0.541
S27E
0.510
I28E
0.286
V30E
1.072
D33E
0.831


E26F
0.331
S27F
0.457
I28F
0.269
V30F
1.219
D33F
1.069


E26G
0.935
S27G
0.594
I28G
0.283
V30G
0.887
D33G
0.971


E26H
0.685
S27H
0.801
I28I
0.566
V30V
0.950
D33H
1.259


E26I
0.655
S27I
0.669
I28I
0.551
V30I
1.107
D33I
1.234


E26K
0.637
S27K
0.674
I28I
0.920
V30K
1.523
D33K
1.215


E26L
0.576
S27L
0.783
I28L
0.518
V30L
1.614
D33L
1.128


E26M
0.462
S27M
0.631
I28M
0.151
V30M
0.939
D33M
−0.034


E26N
0.492
S27N
0.540
I28N
0.143
V30N
0.603
D33N
0.639


E26P
0.485
S27P
0.408
I28P
1.030
V30P
1.079
D33P
0.819


E26Q
0.549
S27Q
0.728
I28I
1.153
V30Q
1.036
D33Q
1.073


E26R
0.455
S27R
0.537
I28R
1.307
V30R
1.130
D33R
1.181


E26S
0.515
S27S
0.539
I28S
1.378
V30S
1.259
D33S
1.223


E26T
0.553
S27V
0.585
I28T
1.455
V30T
1.052
D33T
1.495


E26V
0.646
S27W
0.302
I28V
−0.006
V30V
1.253
D33V
1.455


E26W
0.337
S27Y
0.383
I28W
0.418
V30W
1.392
D33W
1.298


E26Y
0.287


I28Y
1.104
V30Y
1.448
D33Y
1.395


K36A
0.562
R44A
0.997
K50A
0.268
F53A
0.160
L59A
1.247


K36C
0.695
R44C
1.000
K50C
0.430
F53C
0.441
L59C
1.207


K36D
0.583
R44D
0.957
K50D
0.681
F53D
0.715
L59D
1.122


K36E
0.537
R44E
0.818
K50E
0.825
F53E
0.905
L59E
1.193


K36F
0.946
R44F
1.209
K50F
−0.011
F53F
1.055
L59F
1.259


K36G
0.829
R44G
0.531
K50G
0.768
F53G
1.048
L59G
1.276


K36H
0.973
R44H
1.296
K50H
1.178
F53H
1.054
L59H
1.962


K36I
1.194
R44I
1.095
K50I
1.335
F53I
1.262
L59I
1.464


K36K
1.195
R44K
1.039
K50K
1.354
F53K
1.081
L59K
1.485


K36L
1.235
R44L
0.989
K50L
1.334
F53L
1.445
L59L
1.087


K36M
0.775
R44M
0.995
K50M
0.106
F53M
−0.071
L59M
1.216


K36N
0.719
R44N
0.956
K50N
0.348
F53N
0.431
L59N
1.052


K36P
0.605
R44P
0.785
K50P
0.700
F53P
0.874
L59P
0.031


K36Q
0.645
R44Q
0.803
K50Q
0.739
F53Q
0.960
L59Q
1.036


K36R
1.112
R44R
0.776
K50R
−0.003
F53R
1.167
L59R
1.410


K36S
0.975
R44S
0.951
K50S
0.905
F53S
1.117
L59S
0.736


K36T
1.073
R44T
1.268
K50T
1.140
F53T
1.338
L59T
1.184


K36V
1.190
R44V
1.172
K50V
1.113
F53V
1.264
L59V
1.045


K36W
1.008
R44W
0.788
K50W
1.219
F53W
1.273
L59W
1.060


K36Y
1.169
R44Y
1.127
K50Y
1.169
F53Y
1.238
L59Y
0.974


G69A
1.029
S74A
0.699
G78A
0.663
D81A
0.810
G87A
0.879


G69C
0.989
S74C
0.778
G78C
0.837
D81C
0.714
G87C
0.887


G69D
0.844
S74D
0.701
G78D
0.720
D81D
0.011
G87D
0.938


G69E
0.997
S74E
0.717
G78E
0.740
D81E
0.506
G87E
0.812


G69F
0.957
S74F
0.860
G78F
1.011
D81F
0.826
G87F
0.991


G69G
1.132
S74G
0.977
G78G
0.914
D81G
0.796
G87G
−0.006


G69H
1.078
S74H
1.104
G78H
1.180
D81H
0.850
G87H
1.125


G69I
1.102
S74I
1.200
G78I
1.260
D81I
0.685
G87I
1.103


G69K
1.125
S74K
1.243
G78K
1.128
D81K
0.609
G87K
1.342


G69L
1.098
S74L
1.141
G78L
1.335
D81L
0.840
G87L
1.242


G69M
1.162
S74M
0.650
G78M
0.699
D81M
0.701
G87M
1.039


G69N
1.039
S74N
0.724
G78N
0.678
D81N
0.691
G87N
0.804


G69P
0.717
S74P
0.761
G78P
0.764
D81P
0.423
G87P
0.979


G69Q
1.050
S74Q
0.765
G78Q
0.675
D81Q
0.632
G87Q
0.747


G69R
1.056
S74R
0.974
G78R
0.975
D81R
0.616
G87R
1.028


G69S
1.205
S74S
0.945
G78S
0.910
D81S
0.802
G87S
0.961


G69T
1.109
S74T
1.140
G78T
1.179
D81T
0.928
G87T
1.245


G69V
1.080
S74V
1.174
G78V
1.190
D81V
0.854
G87V
1.171


G69W
0.922
S74W
1.217
G78W
1.270
D81W
0.618
G87W
1.114


G69Y
0.936
S74Y
1.130
G78Y
1.313
D81Y
0.799
G87Y
1.170


G99A
0.217
Q116A
1.006
E117A
0.953
S120A
0.823
G121A
0.944


G99C
0.460
Q116C
0.993
E117C
0.905
S120C
0.883
G121C
0.880


G99D
0.695
Q116D
0.966
E117D
0.702
S120D
0.878
G121D
0.752


G99E
0.780
Q116E
0.925
E117E
0.255
S120E
0.880
G121E
0.710


G99F
1.024
Q116F
1.202
E117F
1.208
S120F
1.012
G121F
1.090


G99G
−0.004
Q116G
0.865
E117G
0.703
S120G
0.874
G121G
0.912


G99H
0.289
Q116H
0.922
E117H
1.077
S120H
1.083
G121H
1.145


G99I
1.271
Q116I
1.219
E117I
1.417
S120I
1.202
G121I
1.174


G99K
1.244
Q116K
1.092
E117K
0.553
S120K
1.180
G121K
1.023


G99L
1.228
Q116L
1.117
E117L
1.288
S120L
1.339
G121L
1.147


G99M
0.427
Q116M
0.764
E117M
1.004
S120M
0.963
G121M
0.898


G99N
0.572
Q116N
0.804
E117N
0.817
S120N
0.798
G121N
0.740


G99P
0.797
Q116P
0.911
E117P
0.438
S120P
0.595
G121P
0.829


G99Q
0.937
Q116Q
0.815
E117Q
0.664
S120Q
0.745
G121Q
0.724


G99R
1.092
Q116R
0.908
E117R
0.803
S120R
0.844
G121R
0.866


G99S
1.110
Q116S
1.017
E117S
0.803
S120S
0.694
G121S
0.742


G99T
1.430
Q116T
1.360
E117T
0.864
S120T
1.165
G121T
1.070


G99V
1.319
Q116V
1.472
E117V
0.988
S120V
1.050
G121V
1.026


G99W
1.025
Q116W
1.361
E117W
1.272
S120W
1.144
G121W
1.001


G99Y
1.231
Q116Y
1.325
E117Y
0.936
S120Y
1.072
G121Y
1.069


Q125A
0.928
G127A
0.704
I28A
0.256
L130A
0.283
A139A
0.831


Q125C
0.897
G127C
0.870
I28C
0.101
L130C
0.150
A139C
1.031


Q125D
0.827
G127D
0.853
I28D
0.383
L130D
0.966
A139D
0.874


Q125E
0.732
G127E
0.879
I28E
0.286
L130E
0.961
A139E
0.950


Q125F
0.918
G127F
1.084
I28F
0.269
L130L
1.116
A139F
0.816


Q125G
0.815
G127G
0.000
I28G
0.283
L130G
1.151
A139G
0.973


Q125H
1.020
G127H
1.125
I28I
0.566
L130L
1.092
A139H
1.073


Q125I
1.053
G127I
1.030
I28I
0.551
L130I
1.141
A139I
1.042


Q125K
0.888
G127K
0.582
I28I
0.920
L130K
1.170
A139K
0.301


Q125L
0.926
G127L
1.028
I28L
0.518
L130L
1.200
A139L
0.881


Q125M
1.060
G127M
0.802
I28M
0.151
L130M
0.218
A139M
0.844


Q125N
0.970
G127N
0.744
I28N
0.143
L130L
0.908
A139N
0.830


Q125P
0.990
G127P
0.871
I28P
1.030
L130P
0.761
A139P
1.074


Q125Q
0.690
G127Q
0.709
I28I
1.153
L130Q
0.879
A139Q
0.976


Q125R
0.889
G127R
0.593
I28R
1.307
L130R
1.054
A139R
0.305


Q125S
0.853
G127S
0.959
I28S
1.378
L130S
−0.031
A139S
1.072


Q125T
1.007
G127T
1.185
I28T
1.455
L130L
1.041
A139T
1.291


Q125V
0.957
G127V
1.047
I28V
−0.006
L130V
1.093
A139V
1.067


Q125W
1.051
G127W
0.859
I28W
0.418
L130W
0.885
A139W
0.798


Q125Y
1.010
G127Y
1.008
I28Y
1.104
L130Y
1.289
A139Y
0.562


G153A
0.961
I165A
1.022
E173A
0.936
E174A
0.939
G177A
1.025


G153C
1.001
I165C
0.793
E173C
0.831
E174C
0.828
G177C
0.884


G153D
0.814
I165D
1.030
E173D
0.651
E174D
0.698
G177D
0.786


G153G
0.862
I165E
0.826
E173E
0.813
E174E
0.686
G177E
0.741


G153G
0.918
I165F
1.114
E173F
1.135
E174F
1.110
G177F
0.810


G153G
1.070
I165G
0.991
E173G
1.077
E174G
0.899
G177G
0.933


G153H
−0.042
I165H
1.239
E173H
1.250
E174H
1.196
G177H
1.139


G153G
0.966
I165I
1.036
E173I
1.188
E174I
1.227
G177I
1.175


G153K
0.324
I165K
1.157
E173K
1.119
E174K
1.131
G177K
1.130


G153L
0.323
I165L
1.003
E173L
1.190
E174L
1.162
G177L
1.222


G153M
0.833
I165M
0.911
E173M
1.013
E174M
0.943
G177M
1.008


G153N
0.826
I165N
0.949
E173N
0.885
E174N
0.858
G177N
0.928


G153P
0.317
I165P
0.667
E173P
0.856
E174P
0.685
G177P
1.106


G153Q
0.863
I165Q
0.904
E173Q
0.905
E174Q
0.721
G177Q
0.436


G153R
1.011
I165R
1.050
E173R
1.078
E174R
1.073
G177R
0.898


G153S
1.021
I165S
0.950
E173S
1.006
E174S
0.873
G177S
0.915


G153T
0.899
I165T
1.142
E173T
1.226
E174T
1.145
G177T
1.245


G153V
0.307
I165V
0.611
E173V
1.205
E174V
1.154
G177V
1.260


G153W
1.345
I165W
0.983
E173W
1.228
E174W
1.054
G177W
0.998


G153Y
1.120
I165Y
1.455
E173Y
1.183
E174Y
1.003
G177Y
1.031


E179A
0.956
R194A
0.268
Q197A
0.834
R198A
0.622
V202A
0.921


E179C
0.938
R194C
0.725
Q197C
0.867
R198C
0.735
V202C
0.935


E179D
0.844
R194D
0.016
Q197D
0.892
R198D
0.016
V202D
0.840


E179E
0.000
R194E
0.028
Q197E
0.951
R198R
0.983
V202E
0.846


E179F
1.111
R194F
0.823
Q197F
0.995
R198F
0.083
V202F
1.016


E179G
1.055
R194G
−0.027
Q197G
0.835
R198G
0.242
V202G
0.812


E179H
1.328
R194H
1.157
Q197H
1.042
R198H
0.068
V202H
1.244


E179I
1.403
R194I
0.651
Q197I
1.040
R198I
0.519
V202I
1.147


E179K
1.348
R194K
0.813
Q197K
0.896
R198K
0.837
V202K
1.091


E179L
1.460
R194L
0.869
Q197L
0.909
R198L
0.099
V202L
0.930


E179M
1.007
R194M
0.675
Q197M
1.012
R198M
0.681
V202M
0.928


E179N
0.791
R194N
0.183
Q197N
0.904
R198N
0.233
V202N
0.883


E179P
0.820
R194P
−0.006
Q197P
0.822
R198P
0.006
V202P
0.345


E179Q
0.744
R194Q
0.606
Q197Q
0.748
R198R
0.875
V202Q
0.900


E179R
0.939
R194R
0.928
Q197R
0.857
R198R
1.112
V202R
1.163


E179S
1.067
R194S
0.077
Q197S
0.968
R198S
0.346
V202S
0.849


E179T
1.232
R194T
0.151
Q197T
1.186
R198T
0.418
V202T
1.184


E179V
1.218
R194V
0.425
Q197V
1.153
R198V
0.642
V202V
0.323


E179W
1.264
R194W
0.838
Q197W
0.826
R198R
1.079
V202W
0.984


E179Y
1.140
R194Y
0.915
Q197Y
0.972
R198Y
0.028
V202Y
1.016


Q216A
0.831
I229A
0.621
T240A
0.755
R246A
0.749
T251A
0.738


Q216C
0.810
I229C
0.939
T240C
0.675
R246C
0.610
T251C
0.773


Q216D
0.700
I229D
0.016
T240D
0.034
R246D
0.788
T251D
0.782


Q216E
0.748
I229E
0.010
T240E
0.084
R246E
0.803
T251E
0.788


Q216F
0.873
I229F
0.454
T240F
0.055
R246F
0.296
T251F
0.711


Q216G
0.779
I229G
0.358
T240G
0.346
R246G
0.936
T251G
0.917


Q216H
0.995
I229H
0.574
T240H
0.155
R246H
1.028
T251H
1.039


Q216I
0.944
I229I
0.855
T240I
0.957
R246I
0.398
T251I
0.520


Q216K
0.885
I229K
0.093
T240K
0.016
R246K
1.126
T251K
1.012


Q216L
0.873
I229L
1.000
T240L
0.993
R246L
0.535
T251L
0.695


Q216M
0.715
I229M
0.779
T240M
0.812
R246M
0.645
T251M
0.764


Q216N
0.714
I229N
0.495
T240N
0.693
R246N
0.872
T251N
1.097


Q216P
0.599
I229P
−0.017
T240P
0.013
R246P
0.009
T251P
0.374


Q216Q
0.680
I229Q
0.349
T240Q
0.669
R246Q
0.853
T251Q
0.906


Q216R
0.595
I229R
0.013
T240R
0.006
R246R
1.167
T251R
0.942


Q216S
0.740
I229S
0.453
T240S
0.557
R246S
0.896
T251S
0.996


Q216T
0.917
I229T
0.803
T240T
1.150
R246T
1.038
T251T
1.361


Q216V
0.899
I229V
0.782
T240V
0.950
R246V
0.534
T251V
0.713


Q216W
0.864
I229W
0.082
T240W
0.077
R246W
0.526
T251W
0.730


Q216Y
0.843
I229Y
0.106
T240Y
0.087
R246Y
0.614
T251Y
1.093


H254A
0.796
L260A
0.224
F287A
0.097
V290A
0.167
V299A
0.247


H254C
0.784
L260C
0.248
F287C
−0.002
V290C
0.180
V299V
0.618


H254D
0.848
L260D
0.145
F287D
−0.018
V290D
−0.027
V299D
−0.050


H254E
0.813
L260E
0.108
F287E
−0.019
V290E
−0.018
V299E
0.048


H254F
1.172
L260L
0.892
F287F
0.745
V290F
0.000
V299V
0.816


H254G
0.889
L260G
0.339
F287G
0.017
V290G
0.150
V299G
0.012


H254H
1.325
L260H
0.449
F287H
0.086
V290H
0.040
V299H
−0.034


H254I
1.116
L260I
0.543
F287I
0.011
V290I
0.922
V299I
−0.005


H254K
1.201
L260K
0.003
F287K
0.077
V290K
0.035
V299K
0.034


H254L
0.877
L260L
0.884
F287L
0.389
V290L
0.484
V299L
0.719


H254M
0.679
L260M
0.772
F287M
0.261
V290M
−0.015
V299M
0.222


H254N
0.593
L260L
0.746
F287N
0.009
V290N
−0.026
V299N
0.137


H254P
0.001
L260P
0.400
F287P
−0.014
V290P
−0.033
V299P
−0.039


H254Q
0.678
L260Q
0.611
F287Q
0.033
V290Q
−0.016
V299V
0.603


H254R
1.066
L260R
0.008
F287R
0.005
V290R
0.003
V299R
0.102


H254S
0.874
L260S
0.176
F287S
0.021
V290S
0.164
V299S
0.150


H254T
1.108
L260T
−0.002
F287T
0.036
V290T
0.592
V299T
0.292


H254V
1.160
L260V
0.379
F287V
0.213
V290V
0.893
V299V
0.691


H254W
1.150
L260W
0.741
F287W
0.419
V290W
0.033
V299W
0.021


H254Y
0.966
L260Y
0.659
F287Y
0.185
V290Y
0.018
V299Y
0.013


L303A
0.663
L308A
0.191
D311A
0.800
D323A
0.658
D345A
0.654


L303C
0.737
L308C
0.852
D311D
0.747
D323C
0.624
D345C
0.513


L303D
0.616
L308D
0.760
D311D
0.672
D323D
0.644
D345D
0.527


L303E
0.741
L308E
0.706
D311E
0.688
D323E
0.661
D345E
0.517


L303L
0.990
L308F
0.056
D311F
0.933
D323F
0.613
D345D
0.664


L303G
0.717
L308G
0.842
D311G
0.897
D323G
0.707
D345G
0.700


L303H
0.621
L308H
1.186
D311D
0.800
D323H
−0.013
D345D
0.406


L303I
0.804
L308I
1.180
D311I
0.783
D323I
0.608
D345I
0.528


L303L
1.097
L308K
0.149
D311K
0.977
D323K
0.890
D345K
0.532


L303L
1.104
L308L
1.049
D311L
0.978
D323L
0.768
D345L
0.730


L303M
0.770
L308M
0.017
D311M
−0.008
D323M
0.687
D345M
0.657


L303L
0.812
L308N
0.714
D311D
0.706
D323N
0.681
D345N
0.547


L303P
0.661
L308P
0.203
D311P
−0.016
D323P
−0.012
D345P
0.368


L303Q
0.670
L308Q
0.647
D311Q
0.727
D323Q
0.582
D345Q
0.532


L303R
0.853
L308R
0.938
D311R
0.873
D323R
0.755
D345R
0.558


L303S
0.793
L308S
0.685
D311S
0.916
D323S
0.735
D345S
0.610


L303T
0.876
L308T
0.171
D311T
0.843
D323T
0.694
D345T
0.579


L303V
0.810
L308V
0.594
D311V
0.818
D323V
0.625
D345V
0.587


L303W
0.955
L308W
1.066
D311W
0.987
D323W
0.568
D345W
0.679


L303L
1.064
L308Y
0.918
D311D
1.083
D323Y
0.739
D345Y
0.567


L376A
0.233
Y377A
0.536
K379A
0.856
F388A
−0.016
G389A
0.746


L376C
0.432
Y377C
0.551
K379C
0.775
F388C
−0.034
G389C
0.826


L376D
0.081
Y377D
0.575
K379D
0.695
F388D
0.424
G389D
0.730


L376E
0.076
Y377E
0.565
K379E
0.770
F388F
−0.012
G389E
0.766


L376F
0.918
Y377F
0.950
K379F
0.690
F388F
−0.009
G389F
0.875


L376G
0.136
Y377G
0.690
K379G
0.930
F388G
−0.077
G389G
0.943


L376H
0.488
Y377H
1.119
K379H
1.036
F388H
0.000
G389H
1.125


L376I
1.199
Y377I
1.033
K379I
0.862
F388F
−0.048
G389I
1.034


L376K
0.223
Y377K
0.602
K379K
−0.011
F388K
−0.029
G389K
1.242


L376L
1.236
Y377L
1.105
K379L
0.947
F388L
−0.001
G389L
1.158


L376M
0.649
Y377M
0.586
K379M
0.775
F388F
−0.059
G389M
1.008


L376N
0.157
Y377N
0.567
K379N
0.791
F388F
0.885
G389N
0.963


L376P
0.114
Y377P
0.607
K379P
0.834
F388P
−0.023
G389P
0.822


L376Q
0.479
Y377Q
0.490
K379Q
0.814
F388Q
0.956
G389Q
0.804


L376R
0.119
Y377R
0.231
K379R
1.122
F388R
0.738
G389R
1.059


L376S
0.174
Y377S
0.767
K379S
0.875
F388S
0.682
G389S
1.005


L376T
0.483
Y377T
0.975
K379T
1.088
F388T
0.893
G389T
1.178


L376V
0.601
Y377V
1.171
K379V
1.096
F388V
1.200
G389V
1.122


L376W
0.570
Y377W
1.263
K379W
0.916
F388F
1.285
G389W
0.870


L376Y
1.091
Y377Y
1.226
K379Y
−0.003
F388Y
−0.026
G389Y
1.012


G397A
0.396
Q400A
0.011
F403A
0.720
Q421A
0.911
T426A
0.680


G397C
0.053
Q400C
0.459
F403C
0.673
Q421C
0.005
T426C
0.760


G397D
−0.022
Q400D
−0.017
F403D
0.008
Q421D
0.862
T426D
0.657


G397E
−0.016
Q400E
0.021
F403E
0.178
Q421E
1.241
T426E
0.585


G397F
−0.017
Q400F
−0.014
F403F
0.757
Q421F
0.011
T426F
0.818


G397G
1.047
Q400G
0.088
F403G
0.712
Q421G
1.157
T426G
0.863


G397H
−0.018
Q400H
0.520
F403H
0.781
Q421H
1.257
T426H
0.858


G397I
0.093
Q400I
0.003
F403I
0.932
Q421I
1.086
T426I
1.195


G397K
−0.028
Q400K
−0.016
F403K
−0.006
Q421K
1.115
T426K
0.400


G397L
−0.006
Q400L
0.610
F403L
0.909
Q421L
1.047
T426L
0.817


G397M
0.075
Q400M
0.425
F403M
0.701
Q421M
0.917
T426M
0.658


G397N
−0.004
Q400N
0.430
F403N
0.579
Q421N
0.765
T426N
0.599


G397P
−0.002
Q400P
0.007
F403P
−0.006
Q421P
0.742
T426P
0.462


G397Q
0.003
Q400Q
0.737
F403Q
0.381
Q421Q
1.081
T426Q
0.587


G397R
0.000
Q400R
−0.011
F403R
−0.018
Q421R
1.393
T426R
0.375


G397S
−0.002
Q400S
0.273
F403S
0.806
Q421S
0.835
T426S
0.863


G397T
−0.034
Q400T
0.505
F403T
1.032
Q421T
1.084
T426T
0.044


G397V
0.160
Q400V
0.204
F403V
1.055
Q421V
1.130
T426V
1.054


G397W
−0.029
Q400W
−0.026
F403W
0.053
Q421W
1.048
T426W
0.730


G397Y
−0.004
Q400Y
−0.018
F403Y
0.912
Q421Y
0.905
T426Y
0.479


P430A
0.777
F434A
0.170
N438A
−0.019
A443A
1.166
A445A
0.681


P430C
0.718
F434C
0.232
N438C
−0.054
A443A
1.116
A445C
0.653


P430D
0.002
F434D
0.009
N438D
−0.044
A443A
1.128
A445D
0.619


P430E
0.002
F434E
0.011
N438E
−0.044
A443A
1.154
A445E
0.669


P430F
−0.001
F434F
0.240
N438F
−0.036
A443F
0.283
A445F
0.613


P430G
0.300
F434G
0.032
N438G
−0.070
A443G
0.504
A445G
0.624


P430H
0.023
F434H
0.085
N438H
−0.036
A443H
0.847
A445H
1.183


P430I
0.021
F434I
0.554
N438I
−0.046
A443I
0.625
A445I
0.416


P430K
0.014
F434K
0.053
N438K
−0.057
A443A
1.342
A445K
0.858


P430L
−0.003
F434L
0.480
N438L
−0.053
A443L
0.840
A445L
0.532


P430M
0.002
F434M
0.454
N438M
−0.053
A443A
1.365
A445M
0.596


P430N
−0.009
F434N
0.053
N438N
1.271
A443N
0.818
A445N
0.639


P430P
−0.003
F434P
0.014
N438P
−0.063
A443P
0.059
A445P
0.525


P430Q
0.013
F434Q
0.040
N438Q
−0.063
A443Q
0.665
A445Q
0.737


P430R
0.004
F434R
0.003
N438R
−0.060
A443R
0.435
A445R
0.685


P430S
1.103
F434S
0.102
N438S
−0.089
A443S
0.860
A445S
0.918


P430T
1.096
F434T
0.438
N438T
−0.042
A443T
0.028
A445T
0.698


P430V
1.061
F434V
0.460
N438V
−0.047
A443V
0.397
A445V
0.513


P430W
0.019
F434W
0.006
N438W
−0.046
A443A
1.290
A445W
0.440


P430Y
−0.005
F434Y
0.136
N438Y
−0.062
A443A
1.205
A445Y
0.528


A448A
0.765
E451A
−0.037
A453A
0.901
N454A
0.735
S457A
0.330


A448C
0.826
E451C
−0.041
A453C
0.877
N454C
0.530
S457C
0.466


A448D
0.737
E451D
−0.023
A453D
0.541
N454D
0.555
S457D
0.753


A448E
0.721
E451E
1.127
A453E
0.770
N454E
0.720
S457E
0.738


A448F
0.780
E451F
−0.033
A453F
0.621
N454F
0.490
S457F
0.802


A448G
0.871
E451G
−0.022
A453G
0.258
N454G
0.830
S457G
0.648


A448H
1.264
E451H
−0.022
A453H
0.755
N454H
0.763
S457H
1.160


A448I
1.013
E451I
−0.035
A453I
0.704
N454I
0.313
S457I
0.435


A448K
0.887
E451K
−0.015
A453K
0.448
N454N
1.099
S457K
0.836


A448L
0.855
E451L
−0.045
A453L
0.762
N454L
0.610
S457L
0.615


A448M
0.780
E451E
1.477
A453A
0.836
N454M
0.224
S457M
0.758


A448N
0.794
E451N
−0.060
A453N
0.826
N454N
0.865
S457N
0.816


A448P
0.698
E451P
−0.046
A453P
0.021
N454P
−0.003
S457P
0.138


A448Q
0.822
E451Q
0.056
A453A
0.848
N454Q
0.619
S457Q
1.057


A448R
1.147
E451R
−0.026
A453R
0.586
N454R
−0.027
S457R
1.104


A448S
1.107
E451S
−0.058
A453S
0.848
N454S
0.840
S457S
1.230


A448T
1.354
E451T
−0.041
A453T
0.909
N454T
0.733
S457T
1.086


A448V
1.232
E451V
−0.036
A453V
0.642
N454V
0.630
S457V
0.594


A448W
0.725
E451W
−0.013
A453W
0.885
N454W
0.657
S457W
0.743


A448Y
0.884
E451Y
−0.033
A453Y
−0.013
N454Y
0.671
S457Y
0.989


T462A
0.930
L469A
0.825
N476A
0.018
K487A
1.149
E488A
0.766


T462C
0.845
L469C
0.850
N476C
−0.039
K487C
1.026
E488C
0.664


T462D
0.839
L469L
0.728
N476D
0.728
K487D
0.819
E488D
0.650


T462E
−0.077
L469L
0.768
N476E
0.624
K487E
0.935
E488E
0.661


T462F
1.080
L469F
0.928
N476F
−0.035
K487F
1.119
E488F
0.667


T462G
1.022
L469G
0.748
N476G
−0.017
K487G
1.215
E488G
0.655


T462H
1.261
L469H
0.868
N476H
−0.013
K487H
1.212
E488H
0.641


T462I
1.182
L469I
0.917
N476I
−0.011
K487I
0.992
E488I
0.878


T462K
1.261
L469L
1.002
N476K
−0.046
K487K
1.248
E488K
0.322


T462L
1.037
L469L
0.980
N476L
−0.078
K487L
1.144
E488L
0.756


T462M
0.890
L469L
0.821
N476M
0.705
K487M
1.251
E488M
0.516


T462N
0.809
L469N
0.879
N476N
0.008
K487N
0.492
E488N
0.247


T462P
0.599
L469P
0.835
N476P
0.642
K487P
0.264
E488P
0.166


T462Q
0.827
L469Q
0.787
N476Q
0.835
K487Q
0.925
E488Q
0.613


T462R
0.941
L469R
0.983
N476R
1.126
K487R
1.314
E488R
0.539


T462S
1.058
L469S
0.871
N476S
0.819
K487S
1.122
E488S
0.429


T462T
1.427
L469T
0.875
N476T
1.087
K487T
1.382
E488T
0.648


T462V
1.312
L469V
0.946
N476V
1.144
K487V
1.365
E488V
0.723


T462W
1.141
L469W
1.056
N476W
1.146
K487W
1.380
E488W
0.705


T462Y
1.139
L469Y
0.192
N476Y
1.241
K487Y
0.970
E488Y
0.436


K489A
0.943
L490A
0.897
G491A
1.076
G492A
1.485
S493A
1.031


K489C
0.678
L490C
0.880
G491C
1.053
G492C
1.109
S493C
1.043


K489D
0.618
L490D
0.782
G491D
0.963
G492D
1.242
S493D
−0.003


K489E
0.579
L490E
0.751
G491E
0.939
G492E
1.249
S493E
1.273


K489F
0.926
L490F
1.137
G491F
1.127
G492F
0.945
S493F
0.812


K489G
0.842
L490G
0.861
G491G
0.914
G492G
1.114
S493G
1.205


K489H
1.081
L490H
1.458
G491H
1.413
G492H
1.379
S493H
0.954


K489I
1.210
L490I
1.522
G491I
1.569
G492I
1.262
S493I
1.202


K489K
1.647
L490K
0.940
G491K
1.292
G492K
1.304
S493K
1.228


K489L
1.045
L490L
1.246
G491L
1.358
G492L
1.179
S493L
1.261


K489M
0.231
L490M
1.044
G491M
1.142
G492M
0.889
S493M
1.086


K489N
0.612
L490N
0.954
G491N
1.000
G492N
0.987
S493N
0.794


K489P
0.504
L490P
0.174
G491P
0.206
G492P
0.888
S493P
0.933


K489Q
0.664
L490Q
0.622
G491Q
0.932
G492Q
0.934
S493Q
0.832


K489R
1.236
L490R
0.900
G491R
1.157
G492R
1.064
S493R
1.352


K489S
0.875
L490S
0.985
G491S
1.034
G492S
0.858
S493S
1.195


K489T
1.200
L490T
1.214
G491T
1.326
G492T
1.384
S493T
1.530


K489V
1.138
L490V
1.406
G491V
1.360
G492V
1.367
S493V
1.404


K489W
1.350
L490W
1.343
G491W
1.328
G492W
1.178
S493W
1.287


K489Y
0.830
L490Y
−0.007
G491Y
1.313
G492Y
1.073
S493Y
1.078


L494A
0.687
F495A
1.025
A496A
0.763
K497A
0.841
P498A
0.636


L494C
0.864
F495C
0.948
A496C
0.701
K497C
0.636
P498C
0.746


L494D
0.863
F495D
0.955
A496D
0.744
K497D
0.811
P498D
0.862


L494E
0.856
F495E
0.944
A496E
0.795
K497E
0.718
P498E
0.857


L494L
0.976
F495F
1.074
A496F
0.971
K497F
0.936
P498F
1.017


L494G
0.935
F495G
1.025
A496G
0.819
K497G
0.962
P498G
1.011


L494H
0.825
F495H
1.314
A496H
1.201
K497H
1.035
P498H
1.259


L494I
0.927
F495I
1.147
A496I
1.144
K497I
1.023
P498I
1.107


L494K
1.029
F495K
1.448
A496K
1.030
K497K
1.118
P498K
1.107


L494L
1.056
F495L
1.836
A496L
1.061
K497L
1.029
P498L
1.089


L494L
0.784
F495M
1.254
A496M
0.839
K497M
0.735
P498M
0.864


L494N
0.815
F495N
0.982
A496N
0.664
K497N
0.730
P498N
0.798


L494P
0.734
F495P
0.946
A496P
0.816
K497P
0.691
P498P
0.882


L494Q
0.736
F495Q
1.161
A496Q
0.762
K497Q
0.746
P498Q
0.805


L494R
0.889
F495R
1.285
A496R
1.058
K497R
0.854
P498R
1.121


L494S
0.832
F495S
1.399
A496S
0.933
K497S
0.923
P498S
1.032


L494T
0.425
F495T
1.565
A496T
1.172
K497T
1.133
P498T
1.155


L494V
0.895
F495V
1.379
A496V
1.036
K497V
1.124
P498V
1.105


L494W
0.974
F495W
1.442
A496W
0.982
K497W
0.822
P498W
0.904


L494Y
0.949
F495Y
1.452
A496Y
1.014
K497Y
0.991
P498Y
1.103


Q509A
0.977
Y514A
−0.015
H515H
−0.006
A519A
0.853
T521A
−0.015


Q509C
0.844
Y514C
−0.008
H515E
0.657
A519C
0.502
T521C
0.003


Q509D
0.334
Y514D
0.018
H515F
0.782
A519D
0.334
T521D
0.008


Q509E
0.425
Y514E
0.002
H515G
0.850
A519E
0.394
T521E
0.823


Q509F
0.021
Y514F
0.004
H515H
−0.023
A519F
0.757
T521F
0.000


Q509G
0.767
Y514G
0.013
H515H
−0.015
A519G
0.851
T521G
0.981


Q509H
0.406
Y514H
0.026
H515K
0.984
A519H
0.759
T521H
0.018


Q509I
1.111
Y514I
0.022
H515L
0.146
A519A
0.866
T521I
0.012


Q509K
0.290
Y514K
0.017
H515M
0.777
A519K
0.991
T521K
0.007


Q509L
0.154
Y514L
−0.004
H515N
0.696
A519L
0.658
T521L
0.712


Q509M
1.046
Y514M
−0.016
H515P
−0.015
A519A
0.909
T521M
0.218


Q509N
0.558
Y514N
−0.011
H515Q
0.735
A519A
0.849
T521N
−0.027


Q509P
0.012
Y514P
0.006
H515R
0.961
A519P
0.000
T521P
0.012


Q509Q
0.887
Y514Q
0.018
H515S
0.626
A519Q
0.437
T521Q
0.497


Q509R
−0.002
Y514R
0.001
H515T
0.727
A519R
0.887
T521R
−0.006


Q509S
1.004
Y514S
0.011
H515V
0.777
A519S
0.658
T521S
0.841


Q509T
1.440
Y514T
0.021
H515W
0.966
A519T
0.609
T521T
0.005


Q509V
1.307
Y514V
0.020
H515Y
1.072
A519V
0.451
T521V
0.450


Q509W
−0.003
Y514W
0.009


A519W
1.274
T521W
0.012


Q509Y
−0.006
Y514Y
1.093


A519Y
0.727
T521Y
−0.004


E525A
0.693
R528A
0.491
T536A
0.263
I539A
0.765
L540A
0.998


E525C
0.810
R528C
0.305
T536C
0.272
I539C
0.884
L540C
0.901


E525D
0.827
R528D
0.084
T536D
0.175
I539D
−0.007
L540D
0.931


E525E
0.671
R528E
0.343
T536E
0.143
I539E
0.355
L540E
1.007


E525F
0.770
R528F
0.692
T536F
0.454
I539F
0.651
L540F
1.031


E525G
0.800
R528G
0.333
T536G
0.415
I539G
0.279
L540G
1.052


E525H
0.727
R528H
0.669
T536H
0.457
I539H
0.885
L540H
1.343


E525E
0.829
R528R
0.921
T536I
0.486
I539I
1.169
L540I
1.309


E525K
1.131
R528K
1.047
T536K
0.308
I539K
1.004
L540K
1.278


E525L
0.889
R528L
0.588
T536L
0.625
I539L
1.209
L540L
1.119


E525M
0.788
R528M
0.704
T536M
0.294
I539M
0.960
L540M
1.089


E525N
0.726
R528N
0.349
T536N
0.289
I539N
0.319
L540N
0.975


E525P
0.366
R528P
−0.018
T536P
0.011
I539P
0.530
L540P
0.963


E525Q
0.722
R528R
0.845
T536T
0.303
I539Q
0.637
L540Q
1.095


E525R
0.833
R528R
0.924
T536R
0.149
I539R
0.645
L540R
1.135


E525S
0.638
R528S
0.425
T536S
0.355
I539S
0.895
L540S
1.122


E525T
0.822
R528T
0.595
T536T
0.402
I539T
1.257
L540T
1.580


E525V
0.851
R528V
0.854
T536V
0.382
I539V
1.403
L540V
1.726


E525W
0.942
R528W
0.296
T536T
0.591
I539W
0.644
L540W
1.177


E525E
1.014
R528Y
0.606
T536Y
0.579
I539Y
0.672
L540Y
1.203


R544A
0.832


R544C
1.041


R544D
0.831


R544E
0.843


R544F
0.953


R544G
0.910


R544H
1.113


R544I
1.050


R544K
1.189


R544L
1.022


R544M
0.982


R544N
0.885


R544P
0.951


R544Q
0.865


R544R
0.865


R544S
1.232


R544T
1.331


R544V
1.257


R544W
1.280


R544Y
0.504









Example 4
Analysis of Growth Data for E. Coli Clones Expressing Single-Site Mutants of Isoprene Synthase
This example demonstrates the analysis of growth data by performance index instead of growth index. G-values were determined at regular time intervals using the Enzyscreen Growth Profiler 1152. The growth rate for each sample was calculated by linear regression using Excel. This is given by the rise of the G-value with respect to time (ΔG/Δt). This value is termed “Slope.” The final cell density of each sample was estimated from the final G-value minus the initial G-value. This number is termed “Delta.”
For each 96-well plate, each “Slope” and “Delta” value was normalized by dividing it by the average values of the wells containing the wild-type controls. Several obvious wild-type outliers were rejected when performing this calculation. The normalized values are termed the “Performance Index” or “P.I.”
The “Slope PI” and the “Delta PI” are highly correlated, so an additional PI, called the “Average PI” was calculated from them. This is simply the mean of the two PI values. Table 9 shows the various PI values for all variants at all positions analyzed, and Table 10 shows the top 150 variants with the highest values for normalized average PI.













TABLE 9









Norm








Slope
Norm
Norm


Variant
Slope PI
Delta PI
Avg PI
PI
Delta PI
Avg PI





















A3A
0.841076
0.875095
0.858086
0.7934
0.8064
0.8000


A3C
0.789685
0.806075
0.79788
0.7449
0.7428
0.7438


A3D
0.915678
0.963838
0.939758
0.8638
0.8881
0.8761


A3E
0.955539
1.010676
0.983107
0.9014
0.9313
0.9165


A3F
1.02453
1.05258
1.038555
0.9665
0.9699
0.9682


A3G
0.897839
0.931793
0.914816
0.8469
0.8586
0.8528


A3H
0.965518
1.01314
0.989329
0.9108
0.9336
0.9223


A3I
0.934467
1.003281
0.968874
0.8815
0.9245
0.9032


A3K
1.041742
1.055045
1.048394
0.9827
0.9722
0.9774


A3L
0.897744
0.951514
0.924629
0.8469
0.8768
0.8620


A3M
0.423923
0.463432
0.443677
0.3999
0.4270
0.4136


A3N
0.941676
0.961373
0.951525
0.8883
0.8859
0.8871


A3P
0.897654
0.917002
0.907328
0.8468
0.8450
0.8459


A3Q
1.008068
1.040255
1.024162
0.9509
0.9585
0.9548


A3R
1.042771
1.106812
1.074791
0.9837
1.0199
1.0020


A3S
0.752869
0.811004
0.781936
0.7102
0.7473
0.7290


A3T
1.100956
1.190624
1.14579
1.0386
1.0971
1.0682


A3V
0.409904
0.465896
0.4379
0.3867
0.4293
0.4082


A3W
0.875926
0.941654
0.90879
0.8263
0.8677
0.8472


A3Y
0.97426
1.04765
1.010955
0.9190
0.9654
0.9425


A7A
0.671295
0.589149
0.630222
0.7943
0.6838
0.7385


A7C
0.432727
0.345109
0.388918
0.5120
0.4006
0.4558


A7D
0.128681
0.009861
0.069271
0.1523
0.0114
0.0812


A7E
0.210737
0.160228
0.185483
0.2493
0.1860
0.2174


A7F
0.354182
0.258831
0.306507
0.4191
0.3004
0.3592


A7G
0.666027
0.660635
0.663331
0.7880
0.7668
0.7773


A7H
0.384921
0.340178
0.36255
0.4554
0.3948
0.4249


A7I
0.295123
0.288412
0.291768
0.3492
0.3348
0.3419


A7K
0.016319
0.081347
0.048833
0.0193
0.0944
0.0572


A7L
0.167392
0.202135
0.184764
0.1981
0.2346
0.2165


A7M
0.215773
0.189809
0.202791
0.2553
0.2203
0.2376


A7N
0.319846
0.261297
0.290572
0.3784
0.3033
0.3405


A7P
0.327703
0.261297
0.2945
0.3877
0.3033
0.3451


A7Q
0.276703
0.244041
0.260372
0.3274
0.2833
0.3051


A7R
0.240467
0.251436
0.245952
0.2845
0.2918
0.2882


A7S
0.426395
0.458501
0.442448
0.5045
0.5322
0.5185


A7T
0.411256
0.446176
0.428716
0.4866
0.5179
0.5024


A7V
0.544081
0.557103
0.550592
0.6437
0.6466
0.6452


A7W
0.544657
0.57929
0.561973
0.6444
0.6724
0.6585


A7Y
0.320163
0.350038
0.3351
0.3788
0.4063
0.3927


Y9A
0.257267
0.246507
0.251887
0.2427
0.2271
0.2348


Y9C
0.183255
0.182414
0.182834
0.1729
0.1681
0.1704


Y9D
0.034026
0.091208
0.062617
0.0321
0.0840
0.0584


Y9E
−0.01448
−0.01232
−0.0134
−0.0137
−0.0114
−0.0125


Y9F
1.00751
1.04272
1.025115
0.9504
0.9608
0.9557


Y9G
0.093756
0.108462
0.101109
0.0884
0.0999
0.0943


Y9H
0.618885
0.744448
0.681666
0.5838
0.6860
0.6355


Y9I
0.517363
0.579288
0.548325
0.4880
0.5338
0.5112


Y9K
0.182975
0.187345
0.18516
0.1726
0.1726
0.1726


Y9L
0.371301
0.409199
0.39025
0.3503
0.3771
0.3638


Y9M
0.194273
0.229251
0.211762
0.1833
0.2112
0.1974


Y9N
0.210955
0.229251
0.220103
0.1990
0.2112
0.2052


Y9P
0.342432
0.431386
0.386909
0.3230
0.3975
0.3607


Y9Q
0.176212
0.246507
0.211359
0.1662
0.2271
0.1970


Y9R
0.375158
0.433852
0.404505
0.3539
0.3998
0.3771


Y9S
0.330182
0.352505
0.341343
0.3115
0.3248
0.3182


Y9T
0.434153
0.490547
0.46235
0.4095
0.4520
0.4310


Y9V
0.470581
0.532453
0.501517
0.4439
0.4906
0.4675


Y9W
0.812232
0.84798
0.830106
0.7662
0.7814
0.7739


Y9Y
0.195035
0.310598
0.252816
0.1840
0.2862
0.2357


N12A
0.871587
0.867702
0.869644
0.8222
0.7995
0.8107


N12C
0.916719
0.976164
0.946441
0.8648
0.8995
0.8823


N12D
0.561783
0.626126
0.593955
0.5299
0.5769
0.5537


N12E
0.477841
0.547243
0.512542
0.4508
0.5043
0.4778


N12F
0.567358
0.626125
0.596741
0.5352
0.5769
0.5563


N12G
0.684853
0.717333
0.701093
0.6460
0.6610
0.6536


N12H
0.810808
0.919468
0.865138
0.7649
0.8472
0.8065


N12I
0.756778
0.838119
0.797449
0.7139
0.7723
0.7434


N12K
0.649287
0.722262
0.685774
0.6125
0.6655
0.6393


N12L
0.538939
0.589149
0.564044
0.5084
0.5429
0.5258


N12M
0.814588
0.860305
0.837447
0.7684
0.7927
0.7807


N12N
0.920787
0.909607
0.915197
0.8686
0.8382
0.8532


N12P
0.771118
0.811006
0.791062
0.7274
0.7473
0.7375


N12Q
0.683834
0.724728
0.704281
0.6451
0.6678
0.6566


N12R
0.695487
0.719798
0.707643
0.6561
0.6633
0.6597


N12S
1.002791
1.050116
1.026453
0.9460
0.9676
0.9569


N12T
0.951928
1.057511
1.00472
0.8980
0.9744
0.9367


N12V
0.856073
0.909607
0.88284
0.8076
0.8382
0.8230


N12W
0.459353
0.44864
0.453997
0.4333
0.4134
0.4232


N12Y
−0.00549
0.064091
0.029301
−0.0052
0.0591
0.0273


S13A
0.839388
0.852911
0.84615
0.7918
0.7859
0.7888


S13C
0.879913
0.880027
0.87997
0.8300
0.8109
0.8204


S13D
0.725086
0.766635
0.74586
0.6840
0.7064
0.6953


S13E
0.846514
0.877561
0.862037
0.7985
0.8086
0.8036


S13F
0.97893
0.988489
0.983709
0.9234
0.9108
0.9171


S13G
0.986121
0.9737
0.979911
0.9302
0.8972
0.9135


S13H
1.001156
1.052581
1.026869
0.9444
0.9699
0.9573


S13I
0.998662
1.020535
1.009598
0.9421
0.9404
0.9412


S13K
1.051537
1.069836
1.060686
0.9919
0.9858
0.9888


S13L
0.918125
0.986023
0.952074
0.8661
0.9086
0.8876


S13M
0.872911
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S13N
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S13P
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S13Q
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S13R
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S13S
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S13T
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S13V
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S13W
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S13Y
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Y16A
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Y16C
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Y16D
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Y16E
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Y16F
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Y16G
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Y16H
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Y16I
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Y16K
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Y16L
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Y16M
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Y16N
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Y16P
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Y16Q
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Y16R
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Y16S
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Y16T
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Y16V
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Y16W
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Y16Y
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Y18A
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Y18C
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Y18D
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Y18E
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Y18F
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Y18G
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Y18H
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Y18I
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Y18K
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Y18L
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Y18M
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L20A
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L20C
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L20D
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L20E
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L20F
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L20G
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L20H
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L20I
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L20K
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L20L
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L20M
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L20N
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L20P
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L20Q
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L20R
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L20S
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L20T
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L20Y
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D23A
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D23C
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D23D
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D23E
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D23F
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D23G
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D23H
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D23I
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D23K
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D23L
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D23M
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D23N
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D23P
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D23Q
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D23R
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D23S
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D23T
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D23V
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D23W
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D23Y
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D25A
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D25C
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D25D
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D25E
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D25F
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D25G
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D25H
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D25I
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D25K
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D25L
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D25M
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D25N
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D25P
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D25Q
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D25R
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D25S
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D25T
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D25V
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D25W
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D25Y
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E26A
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E26C
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E26D
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E26E
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E26F
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E26G
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E26H
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E26I
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E26K
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E26L
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E26M
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E26N
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E26P
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E26Q
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E26R
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E26S
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E26T
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E26V
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E26W
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E26Y
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S27A
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S27C
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S27D
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S27E
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S27F
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S27G
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S27H
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S27I
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S27K
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S27L
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S27M
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S27N
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S27P
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S27Q
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S27R
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S27S
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S27T
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S27V
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S27W
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S27Y
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I28A
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I28C
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I28D
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I28E
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I28F
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I28G
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I28I
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I28I
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I28I
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I28I
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I28L
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I28M
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I28P
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I28R
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I28S
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I28T
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I28V
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I28W
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I28Y
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V30A
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V30C
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V30D
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V30E
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V30F
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V30G
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V30I
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V30K
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V30L
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V30M
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V30N
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V30P
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V30Q
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V30R
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V30S
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V30T
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V30V
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V30V
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V30W
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V30Y
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D33A
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D33C
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D33D
0.903864
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D33E
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D33F
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D33G
1.006107
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D33H
1.110918
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1.111329
1.0912
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D33I
1.08364
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1.117411
1.0645
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D33K
1.005204
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D33L
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D33M
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D33N
0.679909
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D33P
0.775789
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D33Q
0.893861
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D33R
0.996353
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D33S
0.981573
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D33T
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D33V
1.069913
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D33W
0.951181
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D33Y
0.918413
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K36A
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K36C
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K36D
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K36E
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K36F
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K36G
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K36H
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K36I
0.921059
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1.0898
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K36K
0.849365
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K36L
0.862057
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K36M
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K36N
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K36P
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K36Q
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K36R
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K36S
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K36T
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K36V
0.888651
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K36W
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K36Y
0.806307
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R44A
1.077139
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R44C
1.08871
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R44D
1.025074
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R44E
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R44F
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R44G
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R44H
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R44I
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R44K
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R44L
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R44M
1.115994
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R44N
1.087271
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R44P
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R44Q
0.935708
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R44R
0.922987
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R44S
1.035378
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R44T
1.094293
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R44V
1.094696
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R44W
0.949379
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R44Y
1.051294
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K50A
0.535496
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K50C
0.694326
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K50D
0.804135
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K50E
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K50F
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K50G
0.954182
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K50H
1.081359
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K50I
1.093654
1.064906
1.07928
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K50K
1.122473
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K50L
1.033651
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K50M
0.325919
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K50N
0.615733
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K50P
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K50Q
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K50R
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K50S
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K50T
1.109699
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K50V
1.036983
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K50W
1.09828
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K50Y
1.036993
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F53A
0.410994
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F53C
0.666376
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F53D
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F53E
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F53F
0.941764
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F53G
1.002614
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F53H
1.014
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F53I
1.110952
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F53K
1.05283
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F53L
0.977713
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F53M
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F53N
0.640621
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F53P
0.879404
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F53Q
0.926589
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F53R
1.02633
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F53S
0.965143
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F53T
1.059278
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F53V
1.015588
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F53W
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F53Y
0.968885
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L59A
1.087587
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1.089804
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L59C
1.115246
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1.144308
1.0751
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L59D
1.08114
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L59E
1.112454
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1.146609
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L59F
1.068131
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1.112123
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L59G
1.100021
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1.100952
1.0604
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L59H
1.223234
1.230066
1.22665
1.1792
1.1727
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L59I
1.162188
1.121602
1.141895
1.1203
1.0693
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L59K
1.117002
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1.111907
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L59L
0.940169
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0.953237
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L59M
1.072535
1.087092
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1.0339
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L59N
1.07878
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L59P
0.19649
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L59Q
1.123362
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L59R
1.163944
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L59S
1.035119
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L59T
1.198452
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1.214258
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L59V
1.058489
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1.069093
1.0204
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L59W
0.987342
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0.971893
0.9518
0.9119
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L59Y
1.014252
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0.9777
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G69A
0.97252
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G69C
0.984973
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G69D
0.923646
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G69E
0.904835
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G69F
0.913936
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G69G
0.953943
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0.950263
0.9570
0.9275
0.9421


G69H
0.975453
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0.968413
0.9786
0.9420
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G69I
0.971462
0.9737
0.972581
0.9746
0.9541
0.9642


G69K
0.985227
1.094487
1.039857
0.9884
1.0725
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G69L
0.934008
0.953978
0.943993
0.9370
0.9348
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G69M
0.96156
0.986023
0.973791
0.9646
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G69N
1.006376
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0.9758
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G69P
0.853303
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0.875292
0.8560
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G69Q
1.004772
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G69R
0.960165
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G69S
1.081175
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G69T
1.123227
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1.184041
1.1268
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G69V
1.014851
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1.038646
1.0181
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1.0297


G69W
0.946529
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0.976137
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G69Y
0.880459
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0.8833
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S74A
0.68747
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0.671588
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0.7611
0.7870


S74C
0.684102
0.65324
0.668671
0.8094
0.7582
0.7836


S74D
0.644298
0.59901
0.621654
0.7623
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S74E
0.61828
0.62859
0.623435
0.7315
0.7296
0.7306


S74F
0.879067
0.870167
0.874617
1.0401
1.0100
1.0249


S74G
0.862921
0.855377
0.859149
1.0210
0.9928
1.0068


S74H
0.922584
0.953978
0.938281
1.0916
1.1073
1.0995


S74I
0.920512
0.934258
0.927385
1.0891
1.0844
1.0867


S74K
0.935737
0.981093
0.958415
1.1071
1.1388
1.1231


S74L
0.817417
0.897282
0.85735
0.9672
1.0415
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S74M
0.625935
0.571894
0.598915
0.7406
0.6638
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S74N
0.597701
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0.588495
0.7072
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S74P
0.649421
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0.7684
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0.7575


S74Q
0.588923
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S74R
0.879901
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1.0411
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S74S
0.812438
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0.9613
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S74T
0.959568
1.025465
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1.1353
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S74V
0.978132
1.035325
1.006729
1.1573
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S74W
0.951107
1.003279
0.977193
1.1253
1.1645
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S74Y
0.811517
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0.9602
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G78A
0.560775
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0.6635
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G78C
0.628641
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0.6015
0.7438
0.6667
0.7049


G78D
0.545693
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0.52305
0.6457
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G78E
0.534382
0.488082
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G78F
0.881369
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G78G
0.709545
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G78H
0.964058
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1.1407
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1.1325


G78I
0.987889
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1.1689
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G78K
0.870893
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G78L
0.874721
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0.877374
1.0350
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1.0281


G78M
0.541348
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G78N
0.519761
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G78P
0.583685
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G78Q
0.493628
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G78R
0.888881
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1.0517
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G78S
0.747824
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0.9041
0.8946


G78T
0.954445
1.010674
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1.1293
1.1731
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G78V
0.936888
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0.984874
1.1085
1.1989
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G78W
0.927391
0.995885
0.961638
1.0973
1.1559
1.1269


G78Y
0.911705
0.921932
0.916818
1.0787
1.0701
1.0744


D81A
0.916483
0.867702
0.892092
0.9034
0.8098
0.8553


D81C
0.925517
0.968769
0.947143
0.9123
0.9041
0.9081


D81D
−0.03069
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−0.05355
−0.0303
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D81E
0.937051
0.944118
0.940584
0.9236
0.8811
0.9018


D81F
1.001651
0.99342
0.997535
0.9873
0.9271
0.9564


D81G
1.040707
1.064906
1.052807
1.0258
0.9939
1.0094


D81H
1.004087
1.025465
1.014776
0.9897
0.9571
0.9729


D81I
0.846262
0.835655
0.840959
0.8341
0.7799
0.8063


D81K
0.976405
1.015605
0.996005
0.9624
0.9479
0.9549


D81L
0.928099
0.941654
0.934876
0.9148
0.8788
0.8963


D81M
0.855268
0.872631
0.86395
0.8430
0.8144
0.8283


D81N
0.865179
0.892351
0.878765
0.8528
0.8328
0.8425


D81P
0.811314
0.81347
0.812392
0.7997
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L130L
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L130P
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L130Q
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A139D
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A139E
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A139F
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G153Q
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I165C
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I165G
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I165Q
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I165S
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I165V
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E173C
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E173D
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E173E
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E173F
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E173G
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E173H
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E173I
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E173K
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E173L
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E173M
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E173N
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E173P
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E173Q
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E173R
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E173S
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E173T
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E173V
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E173W
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E173Y
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E174A
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E174C
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E174D
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E174E
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E174F
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E174G
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E174H
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E174I
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E174K
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E174L
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E174M
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E174N
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E174P
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E174Q
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E174R
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E174S
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E174T
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E174V
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E174W
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E174Y
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G177A
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G177C
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G177D
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G177E
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G177F
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G177G
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G177H
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G177I
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G177K
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G177L
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G177M
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G177N
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G177P
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G177Q
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G177R
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G177T
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G177V
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E179A
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E179C
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E179D
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E179G
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E179H
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E179I
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R194A
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R194C
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R194E
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R194F
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R194G
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R194H
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R194I
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R194K
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R194L
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R194M
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R194N
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R194P
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R194Q
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R194R
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R194S
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R194T
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R194V
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R194W
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R194Y
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Q197A
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Q197C
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Q197D
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Q197E
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Q197F
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Q197G
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Q197H
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Q197I
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Q197K
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Q197L
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Q197M
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Q197N
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Q197P
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Q197Q
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Q197R
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Q197S
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Q197T
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Q197V
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Q197W
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Q197Y
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R198A
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R198C
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R198D
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R198F
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R198G
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R198H
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R198I
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R198K
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R198L
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R198M
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R198N
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R198P
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R198R
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R198R
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R198R
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R198R
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R198S
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R198T
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R198V
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R198Y
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V202A
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V202C
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V202D
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V202E
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V202F
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V202G
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V202H
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V202I
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V202M
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V202N
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V202P
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V202Q
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V202R
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V202S
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V202T
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V202V
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V202W
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Q216A
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Q216C
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Q216D
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Q216F
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Q216I
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Q216K
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Q216L
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Q216M
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Q216N
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Q216Q
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T251M
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H254A
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L260A
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L260I
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L260K
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L260L
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L260L
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L260L
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L260M
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L260P
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L260Q
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L260R
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L260S
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F287I
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F287M
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V290A
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V290G
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V290H
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V290I
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L303G
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L303L
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L303T
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L308A
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L308C
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L308Q
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L308S
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D311D
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D311D
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D311D
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D323A
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D323C
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D323D
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D345N
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D345Q
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D345R
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L376A
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L376G
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L376H
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L376I
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L376K
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L376L
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L376M
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L376N
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L376P
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L376Q
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L376R
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L376S
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L376T
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L376V
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L376W
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L376Y
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Y377A
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Y377C
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Y377D
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Y377E
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Y377F
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Y377G
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Y377H
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Y377I
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Y377K
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Y377L
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Y377M
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Y377N
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Y377P
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Y377Q
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Y377R
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Y377S
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Y377T
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Y377V
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Y377W
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Y377Y
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K379A
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K379C
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K379D
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K379E
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K379F
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K379G
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K379H
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K379I
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K379K
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K379L
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K379M
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K379N
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K379P
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K379Q
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K379R
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K379S
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K379T
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K379V
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K379W
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Q421A
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Q421K
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Q421Q
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Q421R
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Q421Y
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T426A
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T426C
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T426D
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T426E
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T426F
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T426G
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T426H
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T426I
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T426L
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T426M
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T426Q
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T426R
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T426W
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T426Y
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P430A
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P430C
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P430D
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P430H
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P430I
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P430N
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P430P
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P430Q
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P430R
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P430S
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P430T
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P430V
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P430W
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P430Y
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F434A
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F434C
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F434F
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F434G
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F434I
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F434L
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F434M
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F434N
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F434P
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F434Q
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F434S
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F434T
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F434Y
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N438H
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A445Q
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A448A
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A448C
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A448D
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A448E
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A448F
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A448G
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A448H
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A448I
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A448K
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A448L
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A448M
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A448N
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A448P
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A448Q
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A448R
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A448S
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A448T
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A448V
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A453A
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A453A
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A453C
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A453H
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A453I
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A453K
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A453P
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A453R
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A453S
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A453T
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A453V
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A453W
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A453Y
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N454A
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N454C
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N454D
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N454E
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N454F
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N454G
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N454H
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N454I
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N454L
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N454M
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N454N
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N454N
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N454P
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N454Q
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N454R
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N454S
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N454T
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N454V
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N454W
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N454Y
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S457A
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S457C
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S457D
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S457E
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S457F
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S457G
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S457H
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S457I
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S457K
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S457L
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S457M
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S457N
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S457P
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S457Q
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S457R
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S457S
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S457T
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S457V
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S457W
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S457Y
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T462A
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T462C
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T462D
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T462E
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T462F
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T462G
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T462H
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T462I
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T462K
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T462L
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T462M
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T462N
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T462P
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T462Q
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T462R
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T462S
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T462T
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T462V
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T462W
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T462Y
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L469A
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L469C
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L469F
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L469G
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L469H
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L469I
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L469L
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L469L
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L469L
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L469L
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L469L
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L469N
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L469P
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L469Q
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L469R
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L469S
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L469T
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L469V
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L469W
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L469Y
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N476A
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N476C
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N476D
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N476E
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N476F
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N476G
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N476H
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N476I
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N476K
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N476L
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N476M
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N476N
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N476P
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N476Q
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N476R
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N476S
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N476T
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N476V
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N476W
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N476Y
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K487A
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K487C
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K487D
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K487E
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K487F
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K487G
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K487H
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K487I
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K487K
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K487L
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K487M
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K487N
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K487P
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K487Q
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K487R
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K487S
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K487T
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K487V
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K487W
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K487Y
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E488A
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E488C
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E488D
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E488E
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E488F
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E488G
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E488H
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E488I
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E488K
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E488L
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E488M
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E488N
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E488P
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E488Q
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E488R
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E488S
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E488T
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E488V
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E488W
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E488Y
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K489A
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K489C
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K489D
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K489E
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K489F
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K489G
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K489H
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K489I
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K489K
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K489L
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K489M
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K489N
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K489P
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K489Q
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K489R
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K489S
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K489T
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K489V
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K489W
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K489Y
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L490A
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L490C
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L490D
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L490E
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L490F
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L490G
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L490H
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L490I
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L490K
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L490L
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L490M
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L490N
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L490P
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L490Q
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L490R
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L490S
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L490T
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L490V
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L490W
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L490Y
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G491A
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G491C
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G491D
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G491E
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G491F
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G491G
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G491H
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G491I
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G491K
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G491L
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G491M
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G491N
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G491P
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G491Q
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G491R
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G491S
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G491T
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G491V
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G491W
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G491Y
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G492A
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G492C
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G492D
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G492E
1.028326
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G492F
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G492G
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G492H
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G492I
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G492K
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G492L
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G492M
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G492N
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G492P
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G492Q
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G492R
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G492S
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G492T
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G492V
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G492W
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G492Y
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S493A
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S493C
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S493D
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S493E
0.970159
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S493F
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S493G
1.036472
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S493H
0.931448
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S493I
1.045164
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1.05257
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S493K
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S493L
1.046085
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S493M
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S493N
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S493P
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S493Q
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S493R
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S493S
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S493T
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S493V
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S493W
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S493Y
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L494A
0.84922
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L494C
1.038704
1.02793
1.033317
0.9116
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0.9433


L494D
1.106021
1.089556
1.097788
0.9707
1.0363
1.0022


L494E
1.112208
1.161043
1.136626
0.9761
1.1043
1.0377


L494G
1.129441
1.064906
1.097173
0.9913
1.0129
1.0016


L494H
1.05315
1.025465
1.039307
0.9243
0.9754
0.9488


L494I
1.132167
1.089556
1.110861
0.9937
1.0363
1.0141


L494K
1.18235
1.111743
1.147046
1.0377
1.0574
1.0472


L494L
1.282111
1.267041
1.274576
1.1253
1.2052
1.1636


L494L
1.193277
1.131463
1.16237
1.0473
1.0762
1.0612


L494L
0.997205
0.941652
0.969428
0.8752
0.8957
0.8850


L494N
1.011554
0.949049
0.980302
0.8878
0.9027
0.8949


L494P
0.877713
0.786353
0.832033
0.7703
0.7479
0.7596


L494Q
0.933545
0.845515
0.88953
0.8193
0.8042
0.8121


L494R
1.064744
0.968769
1.016756
0.9345
0.9215
0.9282


L494S
1.045267
0.966303
1.005785
0.9174
0.9191
0.9182


L494T
0.553156
0.458501
0.505829
0.4855
0.4361
0.4618


L494V
1.0636
0.944118
1.003859
0.9335
0.8980
0.9165


L494W
1.114723
0.9737
1.044211
0.9783
0.9261
0.9533


L494Y
1.096308
1.015605
1.055956
0.9622
0.9660
0.9640


F495A
0.852329
0.82826
0.840295
0.9121
0.8379
0.8739


F495C
0.826023
0.845516
0.835769
0.8839
0.8554
0.8692


F495D
0.844183
0.912071
0.878127
0.9033
0.9227
0.9133


F495E
0.855034
0.936722
0.895878
0.9150
0.9476
0.9318


F495F
0.950761
0.958908
0.954834
1.0174
0.9701
0.9931


F495G
0.897775
0.850446
0.87411
0.9607
0.8603
0.9091


F495H
1.014857
0.978629
0.996743
1.0860
0.9900
1.0367


F495I
0.95905
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F495L
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F495M
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F495N
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F495P
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F495Q
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F495R
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F495S
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F495T
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F495V
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F495W
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F495Y
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A496A
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A496C
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A496D
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A496E
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A496F
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A496G
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A496H
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A496I
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A496K
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A496L
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A496M
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A496N
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A496Q
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A496R
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A496S
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A496T
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A496V
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A496W
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A496Y
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K497A
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K497C
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K497D
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K497E
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K497F
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K497G
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K497H
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K497I
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K497K
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K497L
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K497M
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K497N
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K497P
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K497Q
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K497R
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K497S
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K497T
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K497V
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K497W
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K497Y
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P498A
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P498C
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P498D
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P498E
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P498F
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P498G
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P498H
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P498I
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P498K
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P498L
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P498M
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P498N
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P498P
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P498Q
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P498R
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P498S
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P498T
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P498V
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P498W
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P498Y
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Q509A
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Q509C
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Q509D
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Q509E
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Q509F
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Q509G
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Q509H
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Q509I
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Q509K
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Q509L
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Q509M
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Q509N
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Q509P
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Q509Q
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Q509R
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Q509S
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Q509T
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Q509V
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Q509W
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Q509Y
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Y514A
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Y514C
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Y514D
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Y514E
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Y514F
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Y514H
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Y514I
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Y514K
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Y514L
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Y514M
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Y514N
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Y514P
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Y514Q
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Y514R
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Y514S
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Y514T
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Y514V
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Y514W
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Y514Y
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H515A
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H515E
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H515F
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H515G
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H515H
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H515H
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H515H
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H515H
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H515K
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H515L
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H515M
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H515N
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H515P
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H515Q
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H515R
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H515S
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H515T
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H515V
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H515W
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H515Y
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A519A
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A519A
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A519A
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A519A
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A519C
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A519D
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A519E
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A519F
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A519G
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A519H
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A519K
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A519L
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A519P
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A519Q
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A519R
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A519S
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A519T
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A519V
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A519W
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A519Y
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T521A
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T521C
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T521D
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T521E
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T521F
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T521G
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T521H
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T521I
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T521K
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T521L
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T521M
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T521N
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T521P
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T521Q
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T521R
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T521S
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T521T
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T521V
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T521W
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T521Y
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E525A
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E525C
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E525D
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E525E
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E525E
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E525E
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E525F
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E525G
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E525H
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E525K
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E525L
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E525M
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E525N
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E525P
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E525Q
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E525R
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E525S
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E525T
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E525V
0.872496
0.845516
0.859006
0.9096
0.9264
0.9178


E525W
0.901802
0.894817
0.898309
0.9402
0.9804
0.9598


R528A
0.596877
0.63352
0.615199
0.6223
0.6941
0.6573


R528C
0.471068
0.532453
0.50176
0.4911
0.5834
0.5361


R528D
0.253986
0.340178
0.297082
0.2648
0.3727
0.3174


R528E
0.567494
0.668032
0.617763
0.5917
0.7319
0.6601


R528F
0.790137
0.865236
0.827686
0.8238
0.9480
0.8844


R528G
0.531474
0.542314
0.536894
0.5541
0.5942
0.5736


R528H
0.761739
0.815935
0.788837
0.7942
0.8940
0.8428


R528K
0.949743
0.907143
0.928443
0.9902
0.9939
0.9920


R528L
0.76786
0.820865
0.794363
0.8006
0.8994
0.8487


R528M
0.738201
0.732123
0.735162
0.7696
0.8022
0.7855


R528N
0.564926
0.60887
0.586898
0.5890
0.6671
0.6271


R528P
−0.02058
0.064091
0.021753
−0.0215
0.0702
0.0232


R528R
0.922893
0.865236
0.894065
0.9622
0.9480
0.9553


R528R
0.866645
0.857841
0.862243
0.9035
0.9399
0.9213


R528R
0.80489
0.79375
0.79932
0.8392
0.8697
0.8540


R528S
0.631812
0.611334
0.621573
0.6587
0.6698
0.6641


R528T
0.720739
0.677893
0.699316
0.7514
0.7427
0.7472


R528V
0.857453
0.850446
0.85395
0.8940
0.9318
0.9124


R528W
0.495593
0.522592
0.509093
0.5167
0.5726
0.5439


R528Y
0.726729
0.668032
0.69738
0.7577
0.7319
0.7451


T536A
0.836816
0.8184
0.827608
0.5979
0.5998
0.5988


T536C
0.915101
0.912073
0.913587
0.6538
0.6685
0.6611


T536D
0.773756
0.788819
0.781287
0.5528
0.5781
0.5653


T536E
0.729461
0.764169
0.746815
0.5212
0.5601
0.5404


T536F
1.161728
1.21281
1.187269
0.8300
0.8889
0.8591


T536G
1.133349
1.128999
1.131174
0.8098
0.8275
0.8185


T536H
1.145956
1.163509
1.154732
0.8188
0.8528
0.8356


T536I
0.879527
0.884956
0.882242
0.6284
0.6486
0.6384


T536K
0.911618
0.880027
0.895822
0.6513
0.6450
0.6482


T536L
1.174536
1.151183
1.162859
0.8392
0.8437
0.8414


T536M
0.976348
0.971234
0.973791
0.6976
0.7118
0.7046


T536N
0.987918
0.998349
0.993134
0.7059
0.7317
0.7186


T536P
0.279294
0.303202
0.291248
0.1996
0.2222
0.2107


T536R
0.70848
0.653241
0.680861
0.5062
0.4788
0.4927


T536S
1.11752
1.163509
1.140514
0.7985
0.8528
0.8253


T536T
1.249568
1.225134
1.237351
0.8928
0.8979
0.8953


T536T
1.131996
1.121602
1.126799
0.8088
0.8220
0.8153


T536T
1.034716
1.018069
1.026393
0.7393
0.7462
0.7427


T536V
1.137379
1.153648
1.145513
0.8127
0.8455
0.8289


T536Y
1.212182
1.232531
1.222356
0.8661
0.9033
0.8845


I539A
0.799576
0.823331
0.811454
0.8315
0.8919
0.8611


I539C
0.845684
0.875097
0.860391
0.8794
0.9479
0.9130


I539D
0.033062
0.066557
0.049809
0.0344
0.0721
0.0529


I539E
0.541628
0.586683
0.564156
0.5632
0.6355
0.5986


I539F
0.792832
0.845516
0.819174
0.8245
0.9159
0.8693


I539G
0.518784
0.566963
0.542873
0.5395
0.6142
0.5761


I539H
0.790285
0.81347
0.801878
0.8218
0.8812
0.8509


I539I
0.916701
0.944118
0.930409
0.9533
1.0227
0.9873


I539K
0.937916
0.956442
0.947179
0.9754
1.0360
1.0051


I539L
0.95073
0.990954
0.970842
0.9887
1.0734
1.0302


I539M
0.865998
0.845516
0.855757
0.9006
0.9159
0.9081


I539N
0.495955
0.534917
0.515436
0.5158
0.5794
0.5469


I539P
0.64276
0.709937
0.676349
0.6684
0.7690
0.7177


I539Q
0.749304
0.781424
0.765364
0.7792
0.8465
0.8122


I539R
0.747375
0.79375
0.770562
0.7772
0.8598
0.8177


I539S
0.835958
0.875097
0.855527
0.8693
0.9479
0.9078


I539T
0.920394
0.934257
0.927326
0.9571
1.0120
0.9840


I539V
0.980869
1.015604
0.998236
1.0200
1.1001
1.0593


I539W
0.712773
0.751843
0.732308
0.7412
0.8144
0.7771


I539Y
0.695299
0.769099
0.732199
0.7231
0.8331
0.7770


L540A
0.743534
0.754308
0.748921
0.8532
0.8650
0.8591


L540C
0.725959
0.749379
0.737669
0.8331
0.8594
0.8462


L540D
0.812091
0.81347
0.812781
0.9319
0.9329
0.9324


L540E
0.797371
0.803611
0.800491
0.9150
0.9216
0.9183


L540F
0.876809
0.882492
0.879651
1.0062
1.0120
1.0091


L540G
0.777617
0.803611
0.790614
0.8923
0.9216
0.9069


L540H
0.915792
0.929327
0.92256
1.0509
1.0657
1.0583


L540I
0.9617
0.966303
0.964002
1.1036
1.1081
1.1059


L540K
0.88224
0.919468
0.900854
1.0124
1.0544
1.0334


L540L
0.857056
0.875097
0.866077
0.9835
1.0035
0.9935


L540M
0.897116
0.926863
0.911989
1.0295
1.0629
1.0462


L540N
0.834345
0.8677
0.851022
0.9574
0.9951
0.9762


L540P
0.863591
0.899746
0.881669
0.9910
1.0318
1.0114


L540Q
0.843641
0.82826
0.835951
0.9681
0.9498
0.9590


L540R
0.91412
0.934258
0.924189
1.0490
1.0714
1.0602


L540S
0.927158
0.978628
0.952893
1.0639
1.1223
1.0931


L540T
1.02144
1.00821
1.014825
1.1721
1.1562
1.1642


L540V
0.971854
0.956444
0.964149
1.1152
1.0968
1.1060


L540W
0.917478
0.931793
0.924635
1.0528
1.0686
1.0607


L540Y
0.909351
0.919468
0.91441
1.0435
1.0544
1.0490


R544A
0.781221
0.77403
0.777626
0.8965
0.8876
0.8921


R544C
0.838912
0.880027
0.859469
0.9627
1.0092
0.9859


R544D
0.816611
0.855375
0.835993
0.9371
0.9809
0.9590


R544E
0.732804
0.732123
0.732463
0.8409
0.8396
0.8402


R544F
0.872383
0.936722
0.904553
1.0011
1.0742
1.0377


R544G
0.853798
0.912073
0.882935
0.9798
1.0459
1.0129


R544H
0.902236
0.912073
0.907154
1.0353
1.0459
1.0406


R544I
0.884967
0.892351
0.888659
1.0155
1.0233
1.0194


R544K
0.915488
0.986025
0.950756
1.0505
1.1307
1.0907


R544L
0.870343
0.875095
0.872719
0.9987
1.0035
1.0011


R544M
0.802716
0.803609
0.803163
0.9211
0.9216
0.9213


R544N
0.753139
0.79375
0.773445
0.8642
0.9102
0.8873


R544P
0.788391
0.783889
0.78614
0.9047
0.8989
0.9018


R544Q
0.740588
0.737054
0.738821
0.8498
0.8452
0.8475


R544R
0.730537
0.741984
0.73626
0.8383
0.8509
0.8446


R544S
0.894148
0.917002
0.905575
1.0261
1.0516
1.0388


R544T
0.874489
0.840587
0.857538
1.0035
0.9640
0.9837


R544V
0.876547
0.880027
0.878287
1.0059
1.0092
1.0075


R544W
0.874069
0.907142
0.890605
1.0030
1.0403
1.0217


R544Y
0.604547
0.645846
0.625197
0.6937
0.7406
0.7172
















TABLE 10







Top 150 Variants for Norm Avg PI

















Norm








Slope
Norm
Norm


Variant
Slope PI
Delta PI
Avg PI
PI
Delta PI
Avg PI
















G99I
1.161186
1.239926
1.200556
1.1406
1.2481
1.1937


G78I
0.987889
1.037791
1.01284
1.1689
1.2046
1.1869


S74V
0.978132
1.035325
1.006729
1.1573
1.2017
1.1797


L59H
1.223234
1.230066
1.22665
1.1792
1.1727
1.1759


V30L
1.025017
0.963838
0.994427
1.1777
1.1718
1.1747


G69T
1.123227
1.244856
1.184041
1.1268
1.2198
1.1739


E117I
1.187747
1.257181
1.222464
1.1450
1.1986
1.1719


I28S
1.00392
0.9737
0.98881
1.1534
1.1838
1.1686


Q116V
1.189502
1.247321
1.218412
1.1467
1.1892
1.1680


G99K
1.136314
1.21281
1.174562
1.1162
1.2208
1.1679


I28R
1.026571
0.949049
0.98781
1.1794
1.1538
1.1666


G127H
1.147312
1.321273
1.234293
1.1095
1.2182
1.1651


L540T
1.02144
1.00821
1.014825
1.1721
1.1562
1.1642


A448T
1.006534
1.023
1.014767
1.1550
1.1731
1.1641


L59T
1.198452
1.230064
1.214258
1.1553
1.1727
1.1641


L494L
1.282111
1.267041
1.274576
1.1253
1.2052
1.1636


S74T
0.959568
1.025465
0.992516
1.1353
1.1903
1.1631


G99V
1.147162
1.188159
1.16766
1.1268
1.1960
1.1610


V30F
1.016354
0.944118
0.980236
1.1677
1.1479
1.1578


G78V
0.936888
1.03286
0.984874
1.1085
1.1989
1.1541


A448V
0.979666
1.02793
1.003798
1.1242
1.1788
1.1515


G78T
0.954445
1.010674
0.982559
1.1293
1.1731
1.1514


S74W
0.951107
1.003279
0.977193
1.1253
1.1645
1.1451


E173F
1.082143
1.138858
1.1105
1.1170
1.1696
1.1434


N476V
1.085918
1.109277
1.097597
1.1620
1.1222
1.1415


H515Y
1.079383
1.05258
1.065981
1.1253
1.1533
1.1390


Y16I
1.110586
1.183229
1.146907
1.1141
1.1594
1.1370


L490I
1.086573
1.050116
1.068344
1.1299
1.1375
1.1337


G78H
0.964058
0.968769
0.966413
1.1407
1.1245
1.1325


S493T
1.08658
1.08216
1.08437
1.1627
1.0948
1.1278


D311L
1.166364
1.114207
1.140285
1.1224
1.1328
1.1276


G78W
0.927391
0.995885
0.961638
1.0973
1.1559
1.1269


G69S
1.081175
1.190624
1.1359
1.0846
1.1667
1.1261


E173V
1.087401
1.099418
1.093409
1.1225
1.1291
1.1258


L59R
1.163944
1.183229
1.173587
1.1220
1.1281
1.1251


V30E
0.958734
0.941654
0.950194
1.1015
1.1449
1.1232


S74K
0.935737
0.981093
0.958415
1.1071
1.1388
1.1231


G99T
1.124624
1.131463
1.128043
1.1047
1.1390
1.1216


G492E
1.028326
1.126532
1.077429
1.1004
1.1396
1.1206


Q116I
1.151107
1.180763
1.165935
1.1097
1.1257
1.1177


A139T
1.147503
1.220205
1.183854
1.1096
1.1250
1.1175


K50T
1.109699
1.136392
1.123046
1.0900
1.1439
1.1167


T462W
0.950774
0.995884
0.973329
1.0910
1.1420
1.1166


F495L
1.042055
1.104346
1.073201
1.1151
1.1172
1.1162


G491I
1.061556
1.037791
1.049673
1.1039
1.1242
1.1138


E173R
1.074678
1.087092
1.080885
1.1093
1.1165
1.1129


A448R
0.953238
0.986023
0.96963
1.0939
1.1307
1.1123


D33I
1.08364
1.151183
1.117411
1.0645
1.1588
1.1111


K50K
1.122473
1.111741
1.117107
1.1026
1.1191
1.1108


S493L
1.046085
1.089557
1.067821
1.1194
1.1022
1.1106


G127T
1.129834
1.22267
1.176252
1.0926
1.1273
1.1103


E173T
1.078933
1.077231
1.078082
1.1137
1.1063
1.1100


D33F
1.097951
1.133928
1.11594
1.0785
1.1414
1.1096


K487W
1.157698
1.220205
1.188952
1.1076
1.1099
1.1088


A519W
1.028502
1.045185
1.036843
1.0723
1.1452
1.1078


V30K
0.981241
0.892351
0.936796
1.1274
1.0849
1.1061


V30V
0.942098
0.929329
0.935713
1.0824
1.1299
1.1061


L540V
0.971854
0.956444
0.964149
1.1152
1.0968
1.1060


L540I
0.9617
0.966303
0.964002
1.1036
1.1081
1.1059


L490H
1.055247
1.02793
1.041588
1.0974
1.1135
1.1053


D33H
1.110918
1.111741
1.111329
1.0912
1.1191
1.1050


L59Q
1.123362
1.180763
1.152063
1.0829
1.1257
1.1044


E117L
1.132889
1.170903
1.151896
1.0921
1.1163
1.1043


E179G
1.070203
1.087092
1.078647
1.1412
1.0691
1.1037


F53I
1.110952
1.106812
1.108882
1.0913
1.1141
1.1026


E174H
1.05931
1.08216
1.070735
1.0935
1.1114
1.1025


K50W
1.09828
1.119138
1.108709
1.0788
1.1266
1.1024


K36I
0.921059
0.956444
0.938751
1.0898
1.1102
1.1001


Q116W
1.145149
1.148717
1.146933
1.1039
1.0952
1.0995


S74H
0.922584
0.953978
0.938281
1.0916
1.1073
1.0995


L469W
1.210486
1.198019
1.204252
1.0624
1.1395
1.0994


L59E
1.112454
1.180763
1.146609
1.0724
1.1257
1.0992


E173H
1.053843
1.079696
1.06677
1.0878
1.1089
1.0984


L59C
1.115246
1.17337
1.144308
1.0751
1.1187
1.0970


Q125F
1.080593
1.207879
1.144236
1.0417
1.1516
1.0969


E173W
1.061328
1.067372
1.06435
1.0955
1.0962
1.0959


T462I
0.937846
0.971234
0.95454
1.0762
1.1138
1.0950


S493I
1.045164
1.059976
1.05257
1.1184
1.0723
1.0947


L59I
1.162188
1.121602
1.141895
1.1203
1.0693
1.0947


R44M
1.115994
1.136392
1.126193
1.0807
1.1088
1.0947


E525K
1.00776
1.040255
1.024008
1.0507
1.1398
1.0941


L540S
0.927158
0.978628
0.952893
1.0639
1.1223
1.0931


L490W
1.02336
1.032861
1.028111
1.0642
1.1188
1.0910


R544K
0.915488
0.986025
0.950756
1.0505
1.1307
1.0907


I28T
0.970822
0.875097
0.922959
1.1154
1.0639
1.0897


E174I
1.06009
1.055045
1.057568
1.0943
1.0835
1.0889


V30I
0.921087
0.919466
0.920277
1.0583
1.1179
1.0881


K487H
1.134673
1.198019
1.166346
1.0856
1.0897
1.0877


R246R
1.049452
1.074767
1.062109
1.1191
1.0570
1.0868


S74I
0.920512
0.934258
0.927385
1.0891
1.0844
1.0867


Q216A
1.121348
1.114208
1.117778
1.0858
1.0872
1.0865


T462V
0.948208
0.941652
0.94493
1.0881
1.0799
1.0840


Q125V
1.08209
1.178299
1.130195
1.0431
1.1234
1.0835


K489R
1.017588
1.023
1.020294
1.0582
1.1081
1.0827


K487T
1.165469
1.156112
1.160791
1.1150
1.0516
1.0825


L490V
1.02697
1.01314
1.020055
1.0680
1.0975
1.0824


E117W
1.121175
1.136394
1.128784
1.0808
1.0834
1.0821


T462H
0.929841
0.956444
0.943142
1.0670
1.0968
1.0819


K50H
1.081359
1.094487
1.087923
1.0622
1.1017
1.0817


K487F
1.138386
1.180763
1.159575
1.0891
1.0740
1.0814


G491L
1.023542
1.01314
1.018341
1.0644
1.0975
1.0806


Q116N
1.110872
1.141322
1.126097
1.0709
1.0881
1.0796


Q421R
1.164807
1.220205
1.192506
1.0699
1.0885
1.0793


E179S
1.049078
1.059975
1.054526
1.1187
1.0424
1.0790


I165K
1.033234
1.06244
1.047837
1.0665
1.0911
1.0789


N476R
1.023405
1.050116
1.03676
1.0951
1.0623
1.0783


V30Y
0.950876
0.875097
0.912986
1.0925
1.0639
1.0782


E117F
1.095329
1.153648
1.124489
1.0559
1.0999
1.0780


Q116F
1.097661
1.151184
1.124422
1.0581
1.0975
1.0779


G491W
1.031219
0.998349
1.014784
1.0724
1.0814
1.0768


S493G
1.036472
1.03286
1.034666
1.1091
1.0449
1.0761


R44F
1.119938
1.092023
1.10598
1.0845
1.0655
1.0750


D311S
1.074702
1.096952
1.085827
1.0342
1.1153
1.0748


T462K
0.924406
0.949047
0.936727
1.0608
1.0883
1.0746


D311K
1.09767
1.074767
1.086218
1.0563
1.0927
1.0745


G78Y
0.911705
0.921932
0.916818
1.0787
1.0701
1.0744


G397G
1.078447
1.089557
1.084002
1.0797
1.0683
1.0739


N454N
1.198247
1.153648
1.175948
1.0517
1.0973
1.0736


K50I
1.093654
1.064906
1.07928
1.0743
1.0720
1.0731


S493V
1.068649
0.990954
1.029802
1.1435
1.0025
1.0710


G491V
1.012542
1.005745
1.009143
1.0530
1.0895
1.0708


Q116P
1.109346
1.124067
1.116707
1.0694
1.0717
1.0705


E117C
1.090465
1.141323
1.115894
1.0512
1.0881
1.0698


D33V
1.069913
1.079696
1.074805
1.0510
1.0868
1.0687


G78R
0.888881
0.934258
0.91157
1.0517
1.0844
1.0682


A3T
1.100956
1.190624
1.14579
1.0386
1.0971
1.0682


K487K
1.127248
1.161043
1.144146
1.0785
1.0561
1.0670


E117M
1.094063
1.131463
1.112763
1.0547
1.0787
1.0668


L59F
1.068131
1.156114
1.112123
1.0297
1.1022
1.0662


G491C
1.000956
1.00821
1.004583
1.0409
1.0921
1.0660


I165H
1.020528
1.050116
1.035322
1.0534
1.0785
1.0660


L59K
1.117002
1.106812
1.111907
1.0768
1.0552
1.0659


Q116T
1.106554
1.116672
1.111613
1.0667
1.0646
1.0657


D311D
1.097318
1.057511
1.077414
1.0560
1.0752
1.0656


P498C
1.044272
1.069837
1.057054
1.0160
1.1186
1.0654


V30G
0.926009
0.877561
0.901785
1.0639
1.0669
1.0654


E173K
1.024202
1.045185
1.034693
1.0572
1.0734
1.0653


E179I
1.027233
1.055045
1.041139
1.0954
1.0376
1.0653


S493W
1.008353
1.040255
1.024304
1.0790
1.0524
1.0653


G78F
0.881369
0.936722
0.909046
1.0428
1.0873
1.0653


I165Y
1.047089
1.020535
1.033812
1.0809
1.0481
1.0644


I165F
1.014278
1.052581
1.03343
1.0470
1.0810
1.0640


G491T
1.01659
0.988489
1.002539
1.0572
1.0708
1.0638


A443A
0.914207
0.877561
0.895884
1.0700
1.0574
1.0638


Q125W
1.054892
1.163507
1.109199
1.0169
1.1093
1.0634


G491H
1.032527
0.971233
1.00188
1.0737
1.0521
1.0631


L490F
1.017422
0.986025
1.001723
1.0580
1.0681
1.0630


G492A
1.016037
1.02793
1.021984
1.0872
1.0399
1.0629


E174T
1.009482
1.055047
1.032264
1.0420
1.0835
1.0628


D311G
1.079426
1.06737
1.073398
1.0388
1.0852
1.0620









Example 5
Increased Viability of G491S Isoprene Synthase Mutant
The following example shows that isoprene producing Escherichia coli expressing the G491S variant of isoprene synthase have increased viability during fermentation when compared to the cells expressing the wild type isoprene synthase. The experiments were performed at 14 L-fed batch scale.
Strain Construction
The promoter in front of the citrate synthase gene (gltA) in BL21 (Novagen) has been replaced by a constitutive low expression promoter, namely GI1.2 (U.S. Pat. No. 7,371,558). Two wild-type promoters have been described for gltA (Wilde, R, and J. Guest. 1986. J. Gen. Microbiol. 132:3239-3251) and the synthetic promoter was inserted just after the −35 region of the distal promoter. A PCR product was obtained using primers UpgltACm-F and DngltA1.xgiCm-R (see Table 11), and plasmid FRT-gb2-Cm-FRT from Gene Bridges (Heidelberg, Germany) as a template. The PCR product was purified and used in a lambda red-mediated recombination as described by the manufacturer (Gene Bridges, Heidelberg, Germany). Several colonies were selected for further characterization. The promoter region was amplified using primers gltAPromSeqF and gltApromSeqR (see Table 11) and DNA extracted by resuspending a colony in 30 uL H2O, heating at 95 C for 4 min, spinning down, and using 2 uL of that material as a template in a 50 uL reaction. After observing the sequencing results of the PCR product obtained, a colony harboring each of the three different promoters GI1.2, GI1.5 and GI1.6 (U.S. Pat. No. 7,371,558) was saved for further use (Table 11).
Strain MD09-313 was built by transducing CMP258 with a P1 lysate from strain MCM521 and selecting for colonies on Luria-Bertani plates containing 20 ug/ml kanamycin. CMP258 and MCM521 can be made as described below. P1 lysates are prepared according to the method described in Ausubel, et al., Current Protocols in Molecular Biology. John Wiley and Sons, Inc. The kanamycin marker was removed using the protocol recommended by the manufacturer (Gene Bridges, Heidelberg, Germany) to form strain MD09-314.
A P1 lysate was made from strains CMP141, CMP142 and CMP143 and was used to transduce strain MD09-314, to form CMP440, CMP441 and CMP442 respectively (Table 11). The chloramphenicol marker was removed using the protocol recommended by the manufacturer (Gene Bridges, Heidelberg, Germany) to form strains CMP451, CMP452 and CMP453 respectively (Table 11).
For construction of isoprene production strains, the upper MVA pathway plasmid and either pDW34 (containing wild type Isoprene Synthase) or pCHL243 (containing the G491S variant of Isoprene Synthase, see below) were transformed into strain CMP451 by electroporation. Strains were recovered in liquid LB medium for one hour at 37° C., and plated onto selective solid agar medium plates containing 50 μg/ml of carbenicillin and 50 μg/ml of spectinomycin, and incubated overnight at 37° C. Isolates resistant to these antibiotics and harboring either the plasmid containing wild type IspS (strain CMP457, see Table 12 for genotype), or G491S IspS (strain DW415, see Table 12 for genotype), were chosen for further study.
Construction of Strains MCM518-521 and 528-531: Lambda Promoters Driving Integrated mKKDyI
P1 transduction enables movement of up to 100 kb of DNA between bacterial strains (Thomason et al. 2007). A 17,257 bp deletion in E. coli BL21(DE3) was replaced by moving a piece of the bacterial chromosome from E. coli K12 MG1655 to E. coli BL21(DE3) using P1 transduction.
Two strategies were used employing different selectable markers to identify colonies containing the recombined bacterial chromosome. First, we inserted an antibiotic marker in a gene close to the 17,257 bp sequence to be transferred, whose deletion was not likely to be detrimental to the strain. A strain containing that antibiotic marker will likely have the 17,257 bp piece of bacterial chromosome transduced at the same time as the marker. In this case, we inserted a gene encoding kanamycin resistance (“kanR”) into the ybgS gene, encoding a 126 amino acid protein of unknown function. Second, since it is known that a number of genes involved in utilization of galactose are close to pgl in the 17,257 bp piece to be transduced into E. coli BL21(DE3), colonies transduced with a P1 lysate obtained from E. coli K12 MG1655 (which contains the 17,257 bp sequence deleted in E. coli BL21(DE3)) and isolated in M9 medium (6 g/L Na2HPO4, 3 g/L KH2PO4, 0.5 g/L NaCl, 0.5 g/L NH4Cl, 0.1 mM CaCl2, 2 mM MgSO4) containing 0.4% (w/v) galactose will likely contain the 17,257 bp piece of bacterial chromosome.
Primers MCM120 and MCM224 were used to amplify the chloramphenicol resistance (“CmR”) cassette from the GeneBridges FRT-gb2-Cm-FRT template using the Stratagene HerculaseTM II Fusion kit (Agilent Technologies, Stratagene Products Division, La Jolla, Calif.) according to the manufacturer's protocol. Four 50 μL PCR reactions were cycled as follows: 95° C./2 minutes; 30 cycles of 95° C./20 seconds, 55° C./20 seconds, 72° C./1 minute; and 72° C./3 minutes. Reactions were then cooled to 4° C. The four reactions were pooled, loaded onto a Qiagen PCR column according to the manufacturer's protocol and eluted with 60 μL elution buffer (“EB”) at 55° C.
Plasmid pRedET-carbenicillinR (GeneBridges, Heidelberg, Germany) was electroporated into E. coli BL21(DE3) strain MCM446 (CmR, gi1.6mKKDyI A1-3) using standard procedures. Transformants were recovered by shaking for one hour in SOC medium at 30° C. and then selected on LB+50 μg/mL carbenicillin (“LB/carb50”) plates at 30° C. overnight. A carbenicillin-resistant colony was frozen as strain MCM508.
Strain MCM508 was grown from a fresh streak in 5 mL LB/carb50 at 30° C. to an OD600 of  ̃0.5. At that point, 40 mM L-arabinose was added, and the culture was incubated at 37° C. for 1.5 hours. Cells were then harvested by centrifugation, electroporated with 3 μL of purified amplicons as described above, and then recovered in 500 μL SOC medium at 37° C. for 1.5-3 hours. Transformants were selected on LB+10 μg/mL kanamycin (LB/kan10) plates at 37° C.
Recombination of the amplicon at the target locus was confirmed by PCR with primers GB-DW and MCM208. The resulting amplicons were sequenced to identify four clones having the sequences listed below. Four carbenicillin-sensitive clones were frozen as strains MCM518-MCM521.
Strains MCM518-MCM521 were re-streaked onto LB/kan10 and grown overnight at 37° C. Colonies of strains MCM518-MCM521 were picked, cultured in LB/kan10 at 37° C. and electrotransformed with plasmid pCP20, which encodes the yeast Flp recombinase, chloramphenicol and ampicillin resistance genes and confers temperature sensitive replication on host cells (Cherepanov, P. P. et al., Gene 158(1):9-14 (1995)). Cells were recovered in 500 μL SOC medium by shaking at 30° C. for 1 hour. Transformants were selected on LB/carb50 plates at 30° C. overnight. The following morning a colony from each plate was grown at 30° C. in LB/carb50 medium until visibly turbid. The culture was then shifted to 37° C. for at least 3 hours. Cells were streaked from that culture onto LB plates and grown overnight at 37° C.
The following day colonies were patched to LB, LB/carb50 and LB/kan10. Clones that were sensitive to both carbenicillin and kanamycin (i.e., which could not grow on carb50 and kan10) were cultured in liquid LB and frozen as strains MCM528-MCM531. E. coli strains












Strain
Description
Parent







MCM508
BL21 gi1.6-mKKDyI + predet.-carb
MCM446


MCM518
BL21 neo-PL.6-mKKDyI, clone 10
MCM508


MCM519
BL21 neo-PL.0-mKKDyI, clone 11
MCM508


MCM520
BL21 neo-PL.0-mKKDyI (bad RBS in
MCM508



front of mMVK), clone 13


MCM521
BL21 neo-PL.2-mKKDyI, clone 15
MCM508


MCM528
BL21 PL.6-mKKDyI, neoR looped out
MCM518


MCM529
BL21 PL.0-mKKDyI, neoR looped out
MCM519


MCM530
BL21 PL.0-mKKDyI (bad RBS in
MCM520



front of mMVK), neoR looped out


MCM531
BL21 PL.2-mKKDyI, neoR looped out
MCM521









Primer Sequences















Primer name
Sequence (5′ → 3′)









MCM120
AAAGTAGCCGAAGATGACGGTTTGTCACA




TGGAGTTGGCAGGATGTTTGATTAAAAGC




AATTAACCCTCACTAAAGGGCGG




(SEQ ID NO: 11)



 



MCM224
TAAATCTTACCCGGCGCAGAACAGGATAC




CATGTTTTTTTACCTCCTTTGCACCTTCA




TGGTGGTCAGTGCGTCCTGCTGATGTGCT




CAGTATCACCGCCAGTGGTATTTANGTCA




ACACCGCCAGAGATAATTTATCACCGCAG




ATGGTTATCTGTATGTTTTTTATATGAAT




TTAATACGACTCACTATAGGGCTCG 




(SEQ ID NO: 12)



 



GB-DW
AAAGACCGACCAAGCGACGTCTGA




(SEQ ID NO: 13)



 



MCM208
GCTCTGAATAGTGATAGAGTCA




(SEQ ID NO: 14)







 






The assemblies integrated into the chromosomes of strains MCM518-MCM521 include new PL promoters derived from bacteriophage lambda (λ) and the very beginning of the mMVK ORF, with sequences from the Gene Bridges FRT-gb2-Cm-FRT cassette integrated upstream of the promoter/mMVK assembly, as well as the remainder of the mMVK ORF followed by the rest of the lower MVA pathway integron from strain MCM508.








Promoter/mMVK sequence integrated into MCM518 (SEQ ID NO: 15)



aaagaccgaccaagcgacgtctgagagctccctggcgaattcggtaccaataaaagagctttattttcatgatctgtgtgttggtttttgtgtgc


 


ggcgcggaagttcctattctctagaaagtataggaacttcctcgagccctatagtgagtcgtattaaattcatataaaaaacatacagataacca


 


tctgcggtgataaattatctctggcggtgttgacataaataccactggcggtgatactgagcacatcagcaggacgcactgaccaccatgaa


 


ggtgcaaaggaggtaaaaaaacatggtatcctgttctgcgccgggtaagatttacctgttcggtgaacacgccgtagtttatggcgaaactgc


 


aattgcgtgtgcggtggaactgcgtacccgtgttcgcgcggaactcaatgactctatcactattcagagc


 


Promoter/mMVK sequence integrated into MCM519 (SEQ ID NO: 16)


aaagaccgaccaagcgacgtctgagagctccctggcgaattcggtaccaataaaagagctttattttcatgatctgtgtgttggtttttgtgtgc


 


ggcgcggaagttcctattctctagaaagtataggaacttcctcgagccctatagtgagtcgtattaaattcatataaaaaacatacagataacca


 


tctgcggtgataaattatctctggcggtgttgacctaaataccactggcggtgatactgagcacatcagcaggacgcactgaccaccatgaa


 


ggtgcaaaggaggtaaaaaaacatggtatcctgttctgcgccgggtaagatttacctgttcggtgaacacgccgtagtttatggcgaaactgc


 


aattgcgtgtgcggtggaactgcgtacccgtgttcgcgcggaactcaatgactctatcactattcagagc


 


Promoter/mMVK sequence integrated into MCM520 (SEQ ID NO: 17)


aaagaccgaccaagcgacgtctgagagctccctggcgaattcggtaccaataaaagagctttattttcatgatctgtgtgttggtttttgtgtgc


 


ggcgcggaagttcctattctctagaaagtataggaacttcctcgagccctatagtgagtcgtattaaattcatataaaaaacatacagataacca


 


tctgcggtgataaattatctctggcggtgttgacctaaataccactggcggtgatactgagcacatcagcaggacgcactgaccaccatgaa


 


ggtgcaaaggtaaaaaaacatggtatcctgttctgcgccgggtaagatttacctgttcggtgaacacgccgtagtttatggcgaaactgcaatt


 


gcgtgtgcggtggaactgcgtacccgtgttcgcgcggaactcaatgactctatcactattcagagc


 


Promoter/mMVK sequence integrated into MCM521 (SEQ ID NO: 18)


aaagaccgaccaagcgacgtctgagagctccctggcgaattcggtaccaataaaagagctttattttcatgatctgtgtgttggtttttgtgtgc


 


ggcgcggaagttcctattctctagaaagtataggaacttcctcgagccctatagtgagtcgtattaaattcatataaaaaacatacagataacca


 


tctgcggtgataaattatctctggcggtgttgacgtaaataccactggcggtgatactgagcacatcagcaggacgcactgaccaccatgaa


 


ggtgcaaaggaggtaaaaaaacatggtatcctgttctgcgccgggtaagatttacctgttcggtgaacacgccgtagtttatggcgaaactgc


 


aattgcgtgtgcggtggaactgcgtacccgtgttcgcgcggaactcaatgactctatcactattcagagc






Construction of pTrc-MEA-Alba (G491S)-mMVK:

The G491S mutation was introduced into pDW34 (pTrc-MEA-Alba-mMVK) by QuikChange (Stratagene) mutagenesis (see below for PCR cycling parameters). The PCR product was then treated with 1 μl of DpnI (Roche) and incubated at 37° C. to digest the parental DNA template. 1 μl of this solution was then transformed into MCM531 by electroporation using standard molecular biology techniques. Transformed cells were recovered in liquid LB medium for one hour, and then plated onto LB solid agar plates containing 50 μg/ml of carbenicillin and 5 mM mevalonic acid. Plasmids were purified from isolated colonies, and sequenced completely (Quintara Biosciences) to verify the presence of the G491S mutation (in pCHL243, see FIG. 12).
PCR Mixture for QuikChange Reaction

















Template DNA (pTrc-MEA-Alba-mMVK)
~100
ng



G507S QC 2 For
50
uM



G507S QC 2 Rev
50
uM



dNTPs (Roche)
1
ul



10X pfu II fusion buffer
5
ul



Pfu Ultra II
1
ul










Water
add enough to reach




50 ul total










PCR Cycling Parameters for QuikChange Reaction:

95° C.—1 minute

(95° C. 50 seconds, 60° C. 50 seconds, 68° C. 3 minutes) 18 rounds

68° C.-10 minutes








TABLE 11





Primers
















UpgltACm-F
TATTTAATTTTTAATCATCTAATTTGACAATC



ATTCAACAAAGTTGTTACAATTAACCCTCACT



AAAGGGCGG (SEQ ID NO: 19)


 


DngltA1.xgiCm-R
TCAACAGCTGTATCCCCGTTGAGGGTGAGTTT



TGCTTTTGTATCAGCCATATATTCCACCAGCT



ATTTGTTAGTGAATAAAAGTGGTTGAATTATT



TGCTCAGGATGTGGCATHGTCAAGGGCTAATA



CGACTCACTATAGGGCTCG



(SEQ ID NO: 20)


 


gltAPromSeqF
GGCAGTATAGGCTGTTCACAAAATC



(SEQ ID NO: 21)


 


gltApromSeqR
CTTGACCCAGCGTGCCTTTCAGC



(SEQ ID NO: 22)


 


G507S QC 2 For
GAAAAACTGAGTGGTAGCCTGTTCGCGAAAC



(SEQ ID NO: 23)


 


G507S QC 2 Rev
AGGCTACCACTCAGTTTTTCCTTGTTCATCT



(SEQ ID NO: 24)
















TABLE 12





E. coli strains



















Strain
Description
Parent



CMP141
BL21 Cm-GI1.2 gltA
BL21


 



CMP142
BL21 Cm-GI1.5 gltA
BL21


 



CMP143
BL21 Cm-GI1.6 gltA
BL21


 



CMP258
BL21 pgl+
BL21


 



MD09-313
BL21 pgl+ neo-PL.2-mKKDyI
CMP258


 



MCM521
BL21 neo-PL.2-mKKDyI
As described herein


 



MD09-314
BL21 pgl + PL.2-mKKDyI
MD09-313


 



CMP440
BL21 pgl + PL.2 mKKDyI Cm-GI1.2
MD09-314




gltA



 



CMP441
BL21 pgl + PL.2 mKKDyI Cm-GI1.5
MD09-314




gltA



 



CMP442
BL21 pgl + PL.2 mKKDyI Cm-GI1.6
MD09-314




gltA



 



CMP451
BL21 pgl + PL.2 mKKDyI GI1.2 gltA
CMP440


 



CMP452
BL21 pgl + PL.2 mKKDyI GI1.5 gltA
CMP441


 



CMP453
BL21 pgl + PL.2 mKKDyI GI1.6 gltA
CMP442


 



CMP457
BL21 pgl + PL.2 mKKDyI GI1.2 gltA,
CMP451




pDW34 (pTrc-P. alba-mMVK), MCM82





(pCL-Upper MVA pathway)



 



DW415
BL21 pgl + PL.2 mKKDyI GI1.2 gltA,
CMP451




pCHL243 (pTrc-P. alba(G491S)-





mMVK), MCM82 (pCL-Upper MVA





pathway)










Sequence of pCHL243 (SEQ ID NO: 25)





gtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtcaggcagccatcggaagctgtggtatggctgtgcaggtcgtaaat


 


cactgcataattcgtgtcgctcaaggcgcactcccgttctggataatgttttttgcgccgacatcataacggttctggcaaatattctgaaatgag


 


ctgttgacaattaatcatccggctcgtataatgtgtggaattgtgagcggataacaatttcacacaggaaacagcgccgctgagaaaaagcg


 


aagcggcactgctctttaacaatttatcagacaatctgtgtgggcactcgaccggaattatcgattaactttattattaaaaattaaagaggtatat


 


attaatgtatcgattaaataaggaggaataaaccatggaagctcgtcgttctgcgaactacgaacctaacagctgggactatgattacctgctg


 


tcctccgacacggacgagtccatcgaagtatacaaagacaaagcgaaaaagctggaagccgaagttcgtcgcgagattaataacgaaaaa


 


gcagaatttctgaccctgctggaactgattgacaacgtccagcgcctgggcctgggttaccgtttcgagtctgatatccgtggtgcgctggat


 


cgcttcgtttcctccggcggcttcgatgcggtaaccaagacttccctgcacggtacggcactgtctttccgtctgctgcgtcaacacggttttg


 


aggtttctcaggaagcgttcagcggcttcaaagaccaaaacggcaacttcctggagaacctgaaggaagatatcaaagctatcctgagcct


 


gtacgaggccagcttcctggctctggaaggcgaaaacatcctggacgaggcgaaggttttcgcaatctctcatctgaaagaactgtctgaag


 


aaaagatcggtaaagagctggcagaacaggtgaaccatgcactggaactgccactgcatcgccgtactcagcgtctggaagcagtatggt


 


ctatcgaggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagctggcaattctggattacaacatgatccagtctgtataccag


 


cgtgatctgcgtgaaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaactgcactttgctcgtgaccgcctgattgagagcttctac


 


tgggccgtgggtgtagcattcgaaccgcaatactccgactgccgtaactccgtcgcaaaaatgttttctttcgtaaccattatcgacgatatcta


 


cgatgtatacggcaccctggacgaactggagctgtttactgatgcagttgagcgttgggacgtaaacgccatcaacgacctgccggattaca


 


tgaaactgtgctttctggctctgtataacactattaacgaaatcgcctacgacaacctgaaagataaaggtgagaacatcctgccgtatctgac


 


caaagcctgggctgacctgtgcaacgctttcctgcaagaagccaagtggctgtacaacaaatctactccgacctttgacgactacttcggca


 


acgcatggaaatcctcttctggcccgctgcaactggtgttcgcttacttcgctgtcgtgcagaacattaaaaaggaagagatcgaaaacctgc


 


aaaaataccatgacaccatctctcgtccttcccatatcttccgtctgtgcaatgacctggctagcgcgtctgcggaaattgcgcgtggtgaaac


 


cgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaagaactggctaccgaaagcgtgatgaatctgatcgatgaaacctggaa


 


aaagatgaacaaggaaaaactgagtggtagcctgttcgcgaaaccgttcgtggaaaccgcgatcaacctggcacgtcaatctcactgcact


 


tatcataacggcgacgcgcatacctctccggatgagctgacccgcaaacgcgttctgtctgtaatcactgaaccgattctgccgtttgaacgc


 


taactgcataaaggaggtaaaaaaacatggtatcctgttctgcgccgggtaagatttacctgttcggtgaacacgccgtagtttatggcgaaa


 


ctgcaattgcgtgtgcggtggaactgcgtacccgtgttcgcgcggaactcaatgactctatcactattcagagccagatcggccgcaccggt


 


ctggatttcgaaaagcacccttatgtgtctgcggtaattgagaaaatgcgcaaatctattcctattaacggtgttttcttgaccgtcgattccgac


 


atcccggtgggctccggtctgggtagcagcgcagccgttactatcgcgtctattggtgcgctgaacgagctgttcggctttggcctcagcct


 


gcaagaaatcgctaaactgggccacgaaatcgaaattaaagtacagggtgccgcgtccccaaccgatacgtatgtttctaccttcggcggc


 


gtggttaccatcccggaacgtcgcaaactgaaaactccggactgcggcattgtgattggcgataccggcgttttctcctccaccaaagagtta


 


gtagctaacgtacgtcagctgcgcgaaagctacccggatttgatcgaaccgctgatgacctctattggcaaaatctctcgtatcggcgaaca


 


actggttctgtctggcgactacgcatccatcggccgcctgatgaacgtcaaccagggtctcctggacgccctgggcgttaacatcttagaact


 


gagccagctgatctattccgctcgtgcggcaggtgcgtttggcgctaaaatcacgggcgctggcggcggtggctgtatggttgcgctgacc


 


gctccggaaaaatgcaaccaagtggcagaagcggtagcaggcgctggcggtaaagtgactatcactaaaccgaccgagcaaggtctga


 


aagtagattaaagtctagttaaagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcag


 


aacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaa


 


cgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaag


 


actgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacg


 


gcccggagggtggcgggcaggacgcccgccataaactgccaggcatcaaattaagcagaaggccatcctgacggatggcctttttgcgtt


 


tctacaaactctttttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaagga


 


agagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagta


 


aaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaaga


 


acgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcata


 


cactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgcc


 


ataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatggggga


 


tcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggca


 


acaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcagg


 


accacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcact


 


ggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctga


 


gataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggat


 


ctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaag


 


gatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagc


 


taccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaa


 


gaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggac


 


tcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctaca


 


ccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag


 


ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgag


 


cgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggcctt


 


ttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacga


 


ccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcat


 


atggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgcccc


 


gacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagct


 


gcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagcagatcaattcgcgcgcgaaggcgaagcggcatgcatttacgtt


 


gacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaa


 


cgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgg


 


gaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattgg


 


cgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtg


 


gtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaact


 


atccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaa


 


cagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc


 


cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggc


 


gactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagat


 


ggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagaca


 


gctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg


 


gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc


 


cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag


 


cgcgaattgatctg









Fermentation Conditions for Fermentation Number 20100307 and 20100436

Fermentations:

Fermentation 20100307: CMP437
Fermentation 20100437: DW415
Medium Recipe (Per Liter Fermentation Medium):
K2HPO4 7.5 g, MgSO4*7H2O 2 g, citric acid monohydrate 2 g, ferric ammonium citrate 0.3 g, yeast extract 0.5 g, 1000× Modified Trace Metal Solution 1 ml. All of the components were added together and dissolved in Di H2O. This solution was heat sterilized (123° C. for 20 minutes). The pH was adjusted to 7.0 with ammonium hydroxide (28%) and q.s. to volume. Glucose 10 g, Vitamin Solution 8 mL, and antibiotics were added after sterilization and pH adjustment.
1000× Modified Trace Metal Solution (Per Liter):
Citric Acids*H2O 40 g, MnSO4*H2O 30 g, NaCl 10 g, FeSO4*7H2O 1 g, CoCl2*6H2O 1 g, ZnSO*7H2O 1 g, CuSO4*5H2O 100 mg, H3BO3 100 mg, NaMoO4*2H2O 100 mg. Each component was dissolved one at a time in Di H2O, pH was adjusted to 3.0 with HCl/NaOH, and then the solution was q.s. to volume and filter sterilized with a 0.22 micron filter.
Vitamin Solution (Per Liter):
Thiamine hydrochloride 1.0 g, D-(+)-biotin 1.0 g, nicotinic acid 1.0 g, D-pantothenic acid 4.8 g, pyridoxine hydrochloride 4.0 g. Each component was dissolved one at a time in Di H2O, pH was adjusted to 3.0 with HCl/NaOH, and then the solution was q.s. to volume and filter sterilized with 0.22 micron filter.
Feed Solution (Per Kilogram):
Glucose 0.57 kg, Di H2O 0.38 kg, K2HPO4 7.5 g, and 100% Foamblast 10 g. All components were mixed together and autoclaved. Macro Salt Solution 3.4 mL, 1000× Modified Trace Metal Solution 0.8 ml, and Vitamin Solution 6.7 mL were added after the solution had cooled to 25° C.
Macro Salt Solution (Per Liter):
MgS04*7H2O 296 g, citric acid monohydrate 296 g, ferric ammonium citrate 49.6 g. All components were dissolved in water, q.s. to volume and filter sterilized with 0.22 micron filter.
Fermentation of the two strains was performed in a 15-L bioreactor at pH 7.0 and at a temperature of 34° C. Frozen vials of the E. coli strains were thawed and inoculated into tryptone-yeast extract medium (LB) for the bioreactors. After the inoculum grew to an optical density of 1.0, measured at 550 nm (OD550), 500 mL was used to inoculate 15-L bioreactors and bring the initial tank volume to 5 L. The feed solution was fed at an exponential rate (up to 4.5 g/min) for 10 h and then pulse feeding began. Pulses lasted 30 min and were triggered by a pH rise above 7.04. The pulse feedrate was adaptable and equaled the TCER/300. The max rate for a given pulse did not exceed 13.5 g/min. The total amount of glucose delivered to the bioreactor during the fermentations was between 7.4 and 7.5 kg. Induction was achieved by adding a IPTG to the tank to bring the concentration to 200 uM when the cells were at an OD550 of 6.
The isoprene level in the off-gas from the bioreactor was determined using an iSCAN (Hamilton Sundstrand) mass spectrometer.
Experimental Procedure for Membrane Potential and Viability Determination
Membrane potential was used to assess viability of the bacteria during fermentation. Broth from the fermentor was collected and immediately diluted 150-fold into PBS buffer. The cells were then further diluted 150-fold into PBS buffer containing 1 μM bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC4(3), (Invitrogen, Cat. No. B-438). Samples were allowed to stain for 10 minutes before quantification of green fluorescence at the single cell level using flow cytometry (FACSCalibur, Becton Dickinson). An excitation wave length of 488 nm and an emission wave length of 530 nm were used.
Initially, an exponentially growing culture and a heat killed culture of E. coli BL21 was stained with DiBAC4(3) to determine green fluorescence levels from healthy and dead cells respectively. This information was used to create gates for analyzing the flow cytometry data to determine the fraction of cells with intact membrane potential and the fraction of cells without membrane potential. The data was also gated on appropriate cell size (forward scatter versus side scatter measured at 488 nm) to identify only intact bacteria. The level of green fluorescence from the cells passing these criteria was then used to determine the fraction of cells with a healthy membrane potential and the fraction of cells with no membrane potential in the fermentation samples. Cells with intact membrane potential were assumed to be alive and metabolically active, while cells with no membrane potential were assumed to be dead and metabolically inactive.
Results:
Two strains (CMP437 and DW415) producing isoprene via the mevalonic acid pathway were analyzed for their viability during fed batch conditions. The two strains were isogenic except for the presence of a G491S mutation in the isoprene synthase encoded by the DW415 strain. The two strains showed similar growth during the fermentation and produced significant amounts of isoprene. The ratio between the concentration of isoprene (%) and CO2(%) in the offgas shows that strain DM415 expressing the G491S mutant of isoprene synthase has increased levels of isoprene production rate when compared to respiration rate throughout significant parts of the as shown on FIG. 13. The membrane potential of individual bacteria during the fermentation was furthermore determined by staining the bacteria with the DiBAC4(3) dye and analyzing the resulting levels of green fluorescence using flow cytometry. The DiBAC4(3) dye penetrates into bacteria that have lost their membrane potential. Bacteria without a membrane potential are generally assumed to be dead. Strain DW415, expressing the G491S allele of the isoprene synthase, had significantly increased viability throughout most of the fermentation as shown in FIG. 14.
Example 6
Construction of a 6×His-Tagged G491S Variant of P. alba Isoprene Synthase for Crystallization
This Example described the construction of G491S variant P. alba isoprene synthase for crystallization.
The P. alba isoprene synthase (IspS) enzyme harboring the G491S mutation (previously referred to as G507S in non-truncated IspS sequences) was identified by enrichment for variants that better tolerate exposure to high MVA pathway flux within E. coli host cells. To more precisely determine the beneficial property of G491S, the variant enzyme was purified and its crystal structure was solved to a resolution of 2.6 Å and then compared to the structure of the parental, unmodified wild type enzyme.
Methods
The G491S variant was constructed in the vector backbone MD09-163 (encoding the WT enzyme, see FIG. 1), which harbors a TEV protease site and 6×His tag at the C-terminal end of IspS. The G491S variant was generated using the QuikChange Mutagenesis Kit (Stratagene) according to the manufacturer's recommended protocol (see below for PCR reaction and cycling parameters). The QuikChange reaction was transformed into chemically competent E. coli TOP10 cells (Invitrogen) according to the manufacturer's recommended protocol and plated onto LB Kan50 selective media plates. Colonies resistant to kanamycin were directly screened by PCR and verified by sequencing using the primers QB1493 and T7 Reverse (Quintara Biosciences). TOP10 cells were grown in selective medium, and plasmids were purified (Qiagen) and then transformed into chemically competent BL21 DE3 pLysS (Invitrogen) according to the manufacturer's recommended protocol, prior to expression of the IspS variants.
QuikChange Mutagenesis
35 μl H2O
5 μl 10×Pfu Ultra II rxn Buffer
6 μl 2.5 mM dNTPs (Roche)
1 μl 20 μM G507S QC 2 For
1 μl 20 μM G507S QC 2 Rev
1 μl Pfu Ultra II HS Polymerase (Stratagene)
1 μl DNA template (MD09-163)
QuikChange Mutagenesis—Cycling Parameters
1) 95° C.—4 min
2) 95° C.—20 sec
3) 52° C.—20 sec
4) 68° C.—7 min
5) goto Step 2—5×
6) 95° C.—20 sec
7) 55° C.—20 sec
8) 68° C.—7 min
9) goto Step 2—20×
10) 68° C.—10 min
11) 4° C.—forever







TABLE 13





QuikChange and Sequencing Primers


















G507S QC 2 For
gaaaaactgagtggtagcctgttcgcgaaac




(SEQ ID NO: 23)


 



G507S QC 2 Rev
aggctaccactcagtttttccttgttcatct




(SEQ ID NO: 24)


 



T7 Forward
taatacgactcactataggg




(SEQ ID NO: 4)


 



T7 Reverse
gctagttattgctcagcgg




(SEQ ID NO: 5)


 



EL-1000
gcactgtctttccgtctgctgc




(SEQ ID NO: 6)


 



A-rev
ctcgtacaggctcaggatag




(SEQ ID NO: 8)


 



A-rev-2
ttacgtcccaacgctcaact




(SEQ ID NO: 9)


 



QB1493
cttcggcaacgcatggaaat




(SEQ ID NO: 7)
















TABLE 14





Plasmids:
















MD09-163
pET24a-P.alba TRC (MEA WT) C-Term (+) TEV, His tag


pDW100
pET24a-P.alba TRC (MEA G491S) C-Term (+) TEV,



His tag
















TABLE 15





Strains:


















MD09-167
BL21(DE3) pLysS, MD09-163 (WT)


 



DW398
BL21(DE3) pLysS, pDW100 (G491S)










1) Amino Acid Sequence of P. alba MEA(+)TEV - MD09-163 (WT) (SEQ ID NO: 26)


MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFLTLLEL


 


IDNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQHGFEVSQEAF


 


SGFKDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKIGKEL


 


AEQVNHALELPLHRRTQRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLR


 


ETSRWWRRVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFSFVTIIDDIY


 


DVYGTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDKGENILP


 


YLTKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIK


 


KEEIENLQKYHDTISRPSHIFRLCNDLASASAEIARGETANSVSCYMRTKGISEELATESV


 


MNLIDETWKKMNKEKLGGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVL


 


SVITEPILPFERENLYFQGLEHHHHHH


 


DNA sequence of MD09-163 (SEQ ID NO: 27)


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccc


 


tagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttcc


 


gatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccc


 


tttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggat


 


tttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggca


 


cttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcg


 


agcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgag


 


gcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa


 


ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggcca


 


gccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgtta


 


aaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttc


 


taatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagag


 


gcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcat


 


cgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaa


 


tttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatga


 


ccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaa


 


tctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctt


 


cagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgc


 


tctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgca


 


gcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctat


 


gagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggga


 


gcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggggg


 


cggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccct


 


gattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgag


 


gaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctct


 


gatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgc


 


gccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatc


 


accgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcg


 


ttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaag


 


ggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactg


 


gaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaataca


 


gatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagac


 


tttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcg


 


gtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggcc


 


gccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccg


 


aataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtc


 


ctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaag


 


gctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcggga


 


aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcacca


 


gtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcga


 


aaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgc


 


agcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagc


 


atttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccag


 


ccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccac


 


gcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgc


 


aggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgc


 


cgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaa


 


tttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgg


 


gaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtct


 


gataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgc


 


cataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagta


 


ggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgcca


 


ccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagca


 


accgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcacta


 


taggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatggaagctcgtcgttctgcga


 


actacgaacctaacagctgggactatgattacctgctgtcctccgacacggacgagtccatcgaagtatacaaagacaaagcgaaaaagct


 


ggaagccgaagttcgtcgcgagattaataacgaaaaagcagaatttctgaccctgctggaactgattgacaacgtccagcgcctgggcctg


 


ggttaccgtttcgagtctgatatccgtggtgcgctggatcgcttcgtttcctccggcggcttcgatgcggtaaccaagacttccctgcacggta


 


cggcactgtctttccgtctgctgcgtcaacacggttttgaggtttctcaggaagcgttcagcggcttcaaagaccaaaacggcaacttcctgg


 


agaacctgaaggaagatatcaaagctatcctgagcctgtacgaggccagcttcctggctctggaaggcgaaaacatcctggacgaggcga


 


aggttttcgcaatctctcatctgaaagaactgtctgaagaaaagatcggtaaagagctggcagaacaggtgaaccatgcactggaactgcca


 


ctgcatcgccgtactcagcgtctggaagcagtatggtctatcgaggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagctgg


 


caattctggattacaacatgatccagtctgtataccagcgtgatctgcgtgaaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaac


 


tgcactttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagcattcgaaccgcaatactccgactgccgtaactccgtcg


 


caaaaatgttttctttcgtaaccattatcgacgatatctacgatgtatacggcaccctggacgaactggagctgtttactgatgcagttgagcgtt


 


gggacgtaaacgccatcaacgacctgccggattacatgaaactgtgctttctggctctgtataacactattaacgaaatcgcctacgacaacc


 


tgaaagataaaggtgagaacatcctgccgtatctgaccaaagcctgggctgacctgtgcaacgctttcctgcaagaagccaagtggctgta


 


caacaaatctactccgacctttgacgactacttcggcaacgcatggaaatcctcttctggcccgctgcaactggtgttcgcttacttcgctgtcg


 


tgcagaacattaaaaaggaagagatcgaaaacctgcaaaaataccatgacaccatctctcgtccttcccatatcttccgtctgtgcaatgacct


 


ggctagcgcgtctgcggaaattgcgcgtggtgaaaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaagaactggcta


 


ccgaaagcgtgatgaatctgatcgatgaaacctggaaaaagatgaacaaggaaaaactgggtggtagcctgttcgcgaaaccgttcgtgg


 


aaaccgcgatcaacctggcacgtcaatctcactgcacttatcataacggcgacgcgcatacctctccggatgagctgacccgcaaacgcgt


 


tctgtctgtaatcactgaaccgattctgccgtttgaacgcgaaaacctgtattttcagggcctcgagcaccaccaccaccaccactgagatc


 


cggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacggg


 


tcttgaggggttttttgctgaaaggaggaactatatccggat


 


3) Amino Acid Sequence of P. alba MEA G491S (+)TEV - pDW100(G491S) (SEQ ID NO: 28)


MEARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFLTLLEL


 


IDNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQHGFEVSQEAF


 


SGFKDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKIGKEL


 


AEQVNHALELPLHRRTQRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLR


 


ETSRWWRRVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFSFVTIIDDIY


 


DVYGTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDKGENILP


 


YLTKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIK


 


KEEIENLQKYHDTISRPSHIFRLCNDLASASAEIARGETANSVSCYMRTKGISEELATESV


 


MNLIDETWKKMNKEKLSGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVL


 


SVITEPILPFERENLYFQGLEHHHHHH


 


DNA sequence of pDW100 (SEQ ID NO: 29)


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccc


 


tagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttcc


 


gatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccc


 


tttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggat


 


tttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggca


 


cttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcg


 


agcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgag


 


gcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa


 


ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggcca


 


gccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgtta


 


aaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttc


 


taatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagag


 


gcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcat


 


cgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaa


 


tttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatga


 


ccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaa


 


tctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctt


 


cagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgc


 


tctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgca


 


gcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctat


 


gagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggga


 


gcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggggg


 


cggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccct


 


gattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgag


 


gaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctct


 


gatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgc


 


gccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatc


 


accgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcg


 


ttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaag


 


ggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactg


 


gaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaataca


 


gatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagac


 


tttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcg


 


gtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggcc


 


gccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccg


 


aataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtc


 


ctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaag


 


gctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcggga


 


aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcacca


 


gtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcga


 


aaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgc


 


agcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagc


 


atttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccag


 


ccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccac


 


gcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgc


 


aggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgc


 


cgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaa


 


tttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgg


 


gaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtct


 


gataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgc


 


cataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagta


 


ggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgcca


 


ccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagca


 


accgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcacta


 


taggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatggaagctcgtcgttctgcga


 


actacgaacctaacagctgggactatgattacctgctgtcctccgacacggacgagtccatcgaagtatacaaagacaaagcgaaaaagct


 


ggaagccgaagttcgtcgcgagattaataacgaaaaagcagaatttctgaccctgctggaactgattgacaacgtccagcgcctgggcctg


 


ggttaccgtttcgagtctgatatccgtggtgcgctggatcgcttcgtttcctccggcggcttcgatgcggtaaccaagacttccctgcacggta


 


cggcactgtctttccgtctgctgcgtcaacacggttttgaggtttctcaggaagcgttcagcggcttcaaagaccaaaacggcaacttcctgg


 


agaacctgaaggaagatatcaaagctatcctgagcctgtacgaggccagcttcctggctctggaaggcgaaaacatcctggacgaggcga


 


aggttttcgcaatctctcatctgaaagaactgtctgaagaaaagatcggtaaagagctggcagaacaggtgaaccatgcactggaactgcca


 


ctgcatcgccgtactcagcgtctggaagcagtatggtctatcgaggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagctgg


 


caattctggattacaacatgatccagtctgtataccagcgtgatctgcgtgaaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaac


 


tgcactttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagcattcgaaccgcaatactccgactgccgtaactccgtcg


 


caaaaatgttttctttcgtaaccattatcgacgatatctacgatgtatacggcaccctggacgaactggagctgtttactgatgcagttgagcgtt


 


gggacgtaaacgccatcaacgacctgccggattacatgaaactgtgattctggctctgtataacactattaacgaaatcgcctacgacaacc


 


tgaaagataaaggtgagaacatcctgccgtatctgaccaaagcctgggctgacctgtgcaacgctttcctgcaagaagccaagtggctgta


 


caacaaatctactccgacctttgacgactacttcggcaacgcatggaaatcctcttctggcccgctgcaactggtgttcgcttacttcgctgtcg


 


tgcagaacattaaaaaggaagagatcgaaaacctgcaaaaataccatgacaccatctctcgtccttcccatatcttccgtctgtgcaatgacct


 


ggctagcgcgtctgcggaaattgcgcgtggtgaaaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaagaactggcta


 


ccgaaagcgtgatgaatctgatcgatgaaacctggaaaaagatgaacaaggaaaaactgAGTggtagcctgttcgcgaaaccgttcgtg


 


gaaaccgcgatcaacctggcacgtcaatctcactgcacttatcataacggcgacgcgcatacctctccggatgagctgacccgcaaacgc


 


gttctgtctgtaatcactgaaccgattctgccgtttgaacgcgaaaacctgtattttcagggcctcgagcaccaccaccaccaccactgaga


 


tccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacg


 


ggtcttgaggggttttttgctgaaaggaggaactatatccggat









Expression and Purification of IspS-G491S

Expression of 6×His-tagged IspS-G491S

N-terminally 6×His-tagged IspS was expressed and purified from strain DW398. The growth procedure is suitable for histidine tagged enzymes expressed in BL21(λDE3)pLysS cells. A 10 ml of overnight culture was prepared for each 1 L of planned growth. The appropriate antibiotics (50 mg/ml kanamycin, 25 mg/ml chloramphenicol) was added to 10 ml of LB medium in a 25 ml flask and was inoculated with 1 colony from a fresh plate of cells or directly from glycerol frozen cell stock. Cultures were grown at 30° C. overnight with shaking at  ̃220 rpm. Day cultures were prepared in 1 liter of LB medium with appropriate antibiotics for each culture. Each 1 L day culture was inoculated with 10 ml of overnight culture and grown at 30-37° C. with shaking at  ̃220 rpm until the OD600 reached  ̃0.4-0.6. Day cultures were then induced with 400 μM IPTG and allowed to continue growing at 30° C. with shaking at 220 rpm for  ̃5-6 hours. Cells were then harvested by centrifugation at 10,000×g for 10 min, 4° C. Following Harvest, cells were used directly or stored at −80° C. until ready to process
Purification of 6×His-Tagged IspS
For purification of histidine tagged enzymes from BL21(λDE3)pLysS cells, cells were gently resuspended in fresh Lysis buffer (Lysis buffer: Ni wash buffer+0.5 mM PMST, 0.01% Tween-20, 1 mg/ml lysozyme, 0.2 mg/ml DNaseI; Ni wash buffer: 50 mM NaH2PO4, 300 mM NaCl, 20 mM Imidazole, pH 8.0). Approximately 40-50 ml of lysis buffer was used per 1 L of cell pellet. Cells were then incubated on ice for approximately 30 min. The cell suspension was then lysed fully by passing 2-3 times through a french pressure cell (large french press cell at 1200 psi/High setting) until lysate started to look clear. A sample of the lysate was saved for activity assay and gel analysis ( ̃100 μl). The lysate was then clarified by centrifuging the lysate at 30,000×g for 30 min, 4° C. in a Sorvall Discovery 90SE ultracentrifuge. The supernatant was removed and retained. A sample of the “clarified lysate” was saved for activity assay and gel analysis ( ̃100 μl).
The clarified lysate was run over H isTrap HP columns (GE healthcare) using a gradient from 0-100% Ni buffer B. Following loading of the lysate on the column, the column was washed with Ni wash buffer (50 mM NaH2PO4, 300 mm NaCl, 20 mM imidazole, ph 8.0). The his-tagged IspS was then eluted from the column using a gradient from 0-100% Ni elution buffer (50 mM NaH2PO4, 300 mM NaCl, 500 mM imidazole, ph 8.0) and fractions containing the his-tagged IspS were collected. The column was then washed with Ni stripping buffer (20 mM NaH2PO4, 0.5 m NaCl, 50 mM EDTA, ph 7.4). Samples were then analyzed by SDS-PAGE gel (4-12% gel NUPAGE, Invitrogen) according to manufacturer's directions. Desired fractions were concentrated on spin filters (Vivaspin-20, Sartoris,) and then desalted over a Hi Prep 26/10 Desalting column (GE heathcare) packed with Sephadex G25 resin. The G-25 buffer consisted of 50 mM HEPES, 50 mM NaCl, and 1 mM DTT, pH 7.4. Fractions were then analyzed and concentrated. The samples were then stored at −80° C.
TEV Cleavage (IspS-G491S from Strain DW398)
Strain DW398 is described above. Digestion was performed with TurboTEV Protease from Eton Bioscience Inc. One unit of TurboTEV per 10 μg of purified protein was used. The digest was performed at 4° C. overnight. Samples were passed through another Ni column equilibrated in the Ni buffer to remove uncleaved enzyme, tag, TurboTEV protease (which is also tagged), and impurities. The Ni column pass though and washes were analyzed using SDS-PAGE gel (NUPAGE, Invitrogen; FIG. 16) and DMAPP activity assays. Samples containing pure enzyme were pooled and desalted into 50 mM NaCl pH 7.4 buffer containing 1 mM DTT and stored at −80° C.
Crystal Structure Determination
Construct DW398 was purified as described and a concentrated protein solution was then prepared for surveying possible crystallization conditions. The construct was purified independently and surveyed as described below. All in-house crystallization screens were set up using the hanging drop vapor diffusion method. At a minimum, the construct was surveyed using the following commercial screens: the Crystal Screen from Hampton Research (Aliso Viejo, Calif.) and the JCSG+ Suite from Qiagen (Valencia, Calif.).
Initial crystallization screens were setup using the Crystal Screen from Hampton Research and the JCSG+ Suite from Qiagen. Crystals from this construct were observed in numerous conditions; optimization included 100 variations of pH, precipitating agents, concentrations, and inhibitors. From the optimization experiments, ten different DW398 crystals were screened in-house for diffraction. A crystal composed of IspS-G491S protein was obtained that diffracted to 3.5 Å in house. The large, rod-shaped crystals belong to the tetragonal space group P43212, and have unit cell dimensions a=154.75, b=154.75, c=142.20. The crystals were grown by mixing 2.5 μL of protein (10 mg/ml protein) with 2.5 μL of precipitant solution [0.1 M Sodium Malonate pH 7.0, 18% (wt/vol) Polyethylene glycol 3350, 0.2 M Sodium Thiocyanate] and equilibrated against 500 μL of precipitant. Prior to flash-freezing the crystal in liquid nitrogen, the crystals were cryoprotected by swishing through 0.1 M Sodium Malonate pH 7.0, 18% (wt/vol) Polyethylene glycol 3350, 0.2 M Sodium Thiocyanate, and 25% (wt/vol) ethylene glycol.
The crystal was sent to Stanford Synchrotron Radiation Laboratory, and data were collected at Beamline 7-1 to 2.6 Å resolution. Data were integrated using Mosflm (Leslie, A. (1998) J. of Appl. Crystallography 30, 1036-1040) and scaled using SCALA (Collaborative Computational Project, N. (1994) Acta Crystallographica Section D 50, 760-763). The data were phased with MOLREP (Vagin, A., and Teplyakov, A. (1997) J. of Appl. Crystallography 30, 1022-1025), using the previously determined structure of IspS from P. alba as the starting model as described in WO 2009/132220. The crystal contains one dimer in the asymmetric unit with a solvent content of 63%.
Refinement with Refmac5 (Collaborative Computational Project, N. (1994) Acta Crystallographica Section D 50, 760-763) was used with iterative manual rebuilding steps using the visualization program Coot (Emsley, P., et al. (2010) Acta Crystallographica Section D 66, 486-501). During refinement, the geometry of the protein was checked using Molprobity (Davis, I. W., et al. (2007) Nucl. Acids Res., 35:W375-W383). The current model has an RWork value of 23.5% and an RFree value of 28.6%.
The structure consists of a dimer (FIG. 17). Each monomer is comprised of two helical domains, a C-terminal domain containing the active site and N-terminal domain with unknown function. The electron density clearly supports the presence of a serine at position 491 in strain DW398 (FIG. 18). Structural alignment of the wild type IspS and IspS-G491S show that the overall fold is unchanged, however the conformation of the loop containing residues 490-497 does vary between the wild type and variant proteins (FIG. 19). Coordinates are provided in Appendix 1.
Example 7
Primary Specific Activity Assay for P. alba IspS Complete Site Evaluation Libraries (SELs)
Site Evaluation Libraries (SELs) of the entire P. alba isoprene synthase (MEA P. alba) backbone (544 amino acids) were built in the parent vector pCL201 (FIG. 4), and screened for specific activity to identify isoprene synthase (IspS) molecules with improved characteristics. In most cases, the SEL at a given position contained all 20 possible amino acid substitutions including the wild type. Numbering of each library corresponds to the ORF of MEA P. alba (FIG. 20), where the starting methionine is position 1. Individual strains, built in the MD09-170 background, that contained variants for expression were arrayed into microtiter plates such that each well corresponded to a specific amino acid substitution at the given position in MEA P. alba. Microtiter plates contained four SELs, or four positions in MEA P. alba with all possible substitutions. Remaining wells were used for control strains. Plates were grown, induced, and lysed, in order to measure the amount of isoprene produced per the specific amount of IspS protein in each sample. Specific activity values were calculated for all variants in the entire set of SELs.
Methods
Cell Growth and Lysis
Glycerol stocks of MEA P. alba IspS libraries were thawed briefly and inoculated into microtiter plates (Cellstar) containing liquid LB medium with kanamycin at a concentration of 20 ug/ml. Cultures were grown overnight at 250 rpm, 30° C. to saturation in a shaking incubator using the Enzyscreen clamp system (Enzyscreen). The next day, cultures were removed and inoculated using a Liquidator96 pipettor (Rainin Instruments) at a ratio of 1:10 into TM3-glucose medium containing 50 ug/ml kanamycin and 50 uM IPTG. Wild type controls were grown separately and inoculated into each microtiter plate containing TM3-glucose with a titration of IPTG concentrations, from 30 uM to 65 uM, in separate wells. Plates were returned to the shaking incubator at 250 rpm, 30° C. and induced for five hours. Plates were then removed from the incubator and cultures were harvested into polypropylene microtiter plates (Nunc) by centrifugation at 3700 rpm for 20 minutes at 4° C. in a tabletop centrifuge. The supernatant was removed and pellets were stored at −80° C. prior to lysis, DMAPP assay, and protein determination.
Prior to cell lysis, plates were removed from the −80° C. freezer and thawed on the bench for 10 minutes. Pellets were thoroughly resuspended in 200 ul of lysis buffer (100 mM Tris, 100 mM NaCl pH 7.6 buffer, 1 mg/ml BSA, 50 U/ul Epicentre readylyse lysozyme, 0.1 mg/ml DNase, 0.5 mM PMSF/AEBSF, 5 mM MgCl2) using a Biomek automated workstation (Beckman Coulter), removed, and shaken at room temperature at 450 rpm for 30 minutes. Lysates were then spun at 3200 rpm at 4° C. for 10 minutes, and 150 ul of the supernatant was transferred using a Biomek to a new microtiter plate for DMAPP and dot blot assay.
DMAPP Assay
For the DMAPP assay, 25 ul of lysate was added to 75 ul of DMAPP assay buffer (100 mM Tris/100 mM NaCl pH 7.6, 1 mg/ml BSA, 50 mM MgCl2, 1 mM DMAPP) in a 96-well glass block (Zinser) using a Liquidator96 pipettor (Rainin Instruments). Glass blocks were sealed with aluminum foil seals (Beckman Coulter) and incubated at 450 rpm for one minute at room temperature. Blocks were then incubated at 34° C. in a water bath for 30 minutes, and the reaction was stopped by incubation at 70° C. for two minutes. Blocks were cooled briefly before loading onto the GC-MS.
Sealed glass blocks were loaded onto an Agilent 7890a Gas Chromatography (GC) System equipped with a flame ionization detector (FID) and a CTC CombiPAL autosampler. The GC FID method parameters are described below:
Column: ZB-5 ms
Dimensions: 15 m×0.25 mm×0.25 μm
Oven:














Ramp
Temperature
Hold Time


(° C./min)
(° C.)
(min)





0
37
28














Total Run Time:
28
minutes



Front Inlet Temperature:
110°
C.










Split Ratio:
50:1











Flow Rate:
3.4
mL/min



Injection Volume:
100
μL



Headspace Syringe Volume:
1
mL



Detector Temperature
160°
C.



Hydrogen Flow:
40
mL/min



Air Flow:
400
mL/min



Makeup Flow:
0.1
mL/min










Makeup Gas Type:
Helium










Chemstation software (version E.02.00.493) was used to control the GC and Cycle Composer software (version 1.5.2) was used to control the CTC autosampler. The Cycle Composer software was programmed to continuously inject one sample after another in sequence for a total of 48 injections. 0.2% v/v isoprene balanced with nitrogen gas from Air Liquide was used as the standard for determining calibration response factors. Three separate 2 mL vials were filled with the calibration gas and analyzed using the method described above to determine an average response factor. Calculated response factors allowed for the conversion of individual sample peak area counts to isoprene concentrations using Microsoft Excel.
Protein Determination
Prior to the protein determination assay, several wild type samples from each plate were analyzed by GC-MS for isoprene, and protein concentration was back-calculated from the known specific activity of MEA P. alba to determine the average amount of IspS for all samples in the microtiter plate. For the dot blot assay, nitrocellulose membranes (Invitrogen) were soaked in 1×PBS buffer (10 mM Sodium Phosphate, 150 mM NaCl, PH7.8+/−0.2) and equilibrated for at least 5 minutes. Lysates were then diluted in 1×PBS using a Hamilton MicroLab STAR liquid handling workstation to achieve loading concentration between 0.025-0.5 ug of P. alba IspS. Purified standards were added at concentrations between 0.025-1 ug. The blotting unit (Minifold-1, Whatman) was assembled according to the manufacturer's recommended protocol. Vacuum was applied briefly to remove excess 1×PBS buffer. Samples (approximately 200 ul of each) were transferred to the Minifold-1, and vacuum was applied at 20 kPa. After samples were filtered completely, wells were washed once with 200 ul of 1×PBS buffer. After the wash buffer passed completely through the membrane, the vacuum was removed, and membranes were removed carefully with forceps, labeled, and dried on clean filter paper.
Immunodetection of P. alba IspS molecules at each position on nitrocellulose membranes was carried out using the WesternBreeze kit from Invitrogen. Primary monoclonal or polyclonal antibody (anti-mouse against purified P. alba IspS, Prosci Incorporated) was diluted 1:5000 in blocking solution, and secondary antibody (Alexa Fluor 488 goat anti-mouse IgG (H+L), Invitrogen) was diluted to a concentration of 2 ug/ml in blocking solution. Fluorescent spots were quantified using a Storm 860 Molecular Imager (GMI, Inc.) and ImageQuant software (GE Healthcare), according to the manufacturers' recommended protocols, and specific protein concentrations for each sample were determined by comparison to known standards using Microsoft Excel.
Results
Specific activity values were calculated for every variant in the entire set of SELs by dividing the molar amount of isoprene produced in a given amount of time by the specific amount of protein in each sample. Performance index (PI) was calculated by dividing the specific activity of any given variant by the average of several WT specific activity measurements from the same microtiter plate. A variant that displayed a PI value of 1.5 for specific activity, for example, was 50% improved over WT. PIs for protein concentration and isoprene produced were also calculated in the same fashion, and these measurements were used for detailed data analysis.
Table 16 provides precise definitions for locations of the residues listed in Tables 17 and 18. For example, a residue listed as “N-term” in Table 17 or 18, is between residue 1 and 215 of reference sequence MEA P. alba IspS (SEQ ID NO:1).







TABLE 16





Definitions of locations of MEA P. alba IspS amino acid positions


















Location
Definition



N-term
Residues 1-215



Hinge Region
Residues 216-245



C-term
Residues 246-544



N-term helices
Residues 134-179



Dimer Interface
Residues 240-255 and 316-353



Substrate Capture Loops
Residues 441-454 and 515-527



Active Site
Residues within 8 Å of active




site based on PDB 3N0G



Buried
Surface Accessibility below 35%




and located internally



Surface
Residues located on the surface




of the structure



Surface Loop
Residues in loops located on the




surface of the structure










Surface accessibilities and putative functions of amino acids of interest in the structure of MEA P. alba are also listed in Tables 17 and 18. Surface accessibility was calculated using the program MOE, which is written and supported by the Chemical Computing Group, Inc. An estimate for the water-accessible surface area of each residue was determined using a probe having a specified radius. The estimate was then compared against a library of peptides and the ratio between them was reported as the percent surface accessibility. Tables 17 and 18 also list the putative functions of each residue. For example, functions include but aren't limited to metal binding (in the active site), substrate capture, altered loop shape, alternative interactions in a pocket, and dimer formation.
From the primary data, positions in MEA P. alba which tolerated no changes from the wild type residue were identified (see Table 17, FIGS. 23-29). MEA P. alba variants with amino acid substitutions other than wild type at these positions displayed specific activity no higher than 30% (PI≦0.3) of the value of WT, and were therefore functionally inactive. Wild type residues at these positions represent a minimum set that is required for the efficient conversion of DMAPP to isoprene by MEA P. alba. Many of these positions map to or near the active site of MEA P. alba (see FIG. 24), and are putatively involved in but are not limited to metal binding (for substrate orientation), substrate capture, substrate binding, and catalysis. FIG. 25, for example, shows a position that has an unknown role in enzyme function. FIG. 26 shows positions that may be involved in IspS dimer formation, and FIGS. 27 and 28 show positions that are in or interact with the N-terminus and may be involved in loop closure or function of the active site. FIG. 29 shows positions located in the substrate capture loops that tolerate no substitutions.
Variants that displayed specific activity higher than WT in the primary in vitro assay were selected for retesting. Variants were tested following the methods described above, except that polyclonal antibody in addition to monoclonal antibody was used for immunodetection, according to standard biochemical practices. Table 18 lists a set of retested variants that showed higher specific activity (a PI>1.3) than WT. FIG. 30 shows all positions in the crystal structure of IspS where variants displayed increased specific activity upon retest. In comparison to the wild type enzyme, these variants confer specific activity benefits to IspS by alteration/enhancement of the putative functions listed in Table 18. FIG. 31 shows buried positions in or that interact with the N-terminus of IspS and where variants displayed increased specific activity. FIG. 32 shows buried positions that are in or interact with the C-terminus of IspS, and where variants displayed increased specific activity. FIG. 33 shows position 247, where improved variants may positively affect dimerization of IspS, and FIG. 34 shows additional sites at the N-terminus where variants display clear specific activity benefits. FIG. 35 shows the locations of positions on surface loops where variants displayed high specific activity, and FIG. 36 shows positions on the surface of the enzyme that are not in loops, and where variants displayed increased specific activity. FIG. 37 shows positions in proposed substrate capture loops where variants displayed increased specific activity relative to wild type. Particular positions in this region have variants which displayed increased activity, whereas adjacent positions are immutable (see Table 17 and FIG. 29). This indicates that the proposed “substrate capture loops” in IspS are critical in the enzymatic conversion of DMAPP to isoprene, and are highly sensitive to perturbation, which can result in either negative or positive effects on activity. All variants listed in Table 18 and shown in FIGS. 30 through 37 or any combination thereof represent mutations in IspS that allow the enzyme to more efficiently convert DMAPP to isoprene.







TABLE 17







Positions in MEA P. alba displaying PI specific activity


values ≦ 0.3 for all non-WT amino acid substitutions.














% Surface



Residue
Position
Location
Accessibility
Function














R
4
N-term
Not calc.
Conserved twin R presumably needed for






N-term loop closure


Y
9
N-term
65
Based on model, this points into active site and






interacts with D295 (part of DDxxD)


W
243
Dimer
9
Base of dimer interface, in a hydrophobic




Interface

pocket


D
258
N-term
44
Near active site; possible interaction with






closed N-term tail (W244)


R
259
N-term
29
Near active site; possible interaction with






closed N-term tail


E
262
N-term
24
Near active site; possible interaction with






closed N-term tail


W
266
Active
3
Bottom of active site




Site


R
280
Buried
1
Interacts with backbone of P274, possible loop






stabilization


D
294
Active
32
Part of DDxxD motif




Site


D
295
Active
12
Part of DDxxD motif




Site


D
298
Active
55
Part of DDxxD motif




Site


E
305
N-term
19
Possible interaction with R004 when N-term






loop is closed


Y
387
Substrate
7
Substrate capture loop; role unknown




Capture




Loops


S
396
Active
0
points into active site




Site


G
397
Active
0
in active site; allows for kink in helix




Site


R
435
Active
13
points into active site




Site


D
439
Active
5
likely metal binding




Site


E
446
Active
17
likely metal binding




Site


R
449
Substrate
64
Substrate capture loop; role unknown




Capture




Loops


G
450
Substrate
78
Substrate capture loop; role unknown, though




Capture

likely structural




Loops


Y
514
Active
28
probably catalytic residue




Site


D
518
Substrate
Not calc.
Substrate capture loop; role unknown




Capture




Loops
















TABLE 18







Retested variants of MEA P. alba displaying PI specific activity values > 1.3.















Charge
% Surface



Position
Mutation
Location
Change
Accessibility
Function















2
V
N-term
1
Not calc.
alternate surface







interactions


22
K
N-term
1
54
alternate surface







interactions


22
R
N-term
1
54
alternate surface







interactions


36
D
N-term
−2
33
alternate interactions with







nearby residues


36
E
N-term
−2
33
alternate interactions with







nearby residues


36
H
N-term
−1
33
alternate interactions with







nearby residues


36
W
N-term
−1
33
alternate interactions with







nearby residues


43
E
N-term
−2
52
alternate interactions with







nearby residues


58
F
N-term
1
20
alternate interactions with







nearby residues


71
I
Buried
−1
3
alternate interactions in







hydrophobic pocket


89
D
Buried
−1
5
alternate interactions in







pocket


89
E
Buried
−1
5
alternate interactions in







pocket


118
E
Buried
−1
10
alternate interactions in







pocket


118
P
Buried
0
10
alternate interactions in







pocket


120
M
surface
0
52
alternate surface




loop


interactions


120
Q
surface
0
52
alternate surface




loop


interactions


151
F
surface
0
14
alternate interactions in




loop


pocket


151
Y
surface
0
14
alternate interactions in




loop


pocket


153
P
surface
0
40
affect shape of loop




loop


161
C
Buried
−1
10
alternate interactions in







pocket


228
Y
Buried
0
5
alternate interactions in







pocket


234
R
Hinge
1
24
alternate surface




region


interactions


247
I
dimer
0
5
alternate interactions in




interface


pocket


247
L
dimer
0
5
alternate interactions in




interface


pocket


247
M
dimer
0
5
alternate interactions in




interface


pocket


254
C
surface
0
89
alternate surface




loop


interactions


268
I
Buried
0
2
alternate interactions in







pocket


282
H
Buried
0
8
alternate interactions in







pocket


282
W
Buried
0
8
alternate interactions in







pocket


288
A
Buried
0
0
alternate interactions in







pocket


288
T
Buried
0
0
alternate interactions in







pocket


288
Y
Buried
0
0
alternate interactions in







pocket


331
P
Buried
0
0
alternate interactions in







pocket


348
Y
Dimer
−1
16
alternate surface




interface


interactions


376
L
surface
0
63
alternate surface







interactions


380
E
surface
−1
33
alternate surface




loop


interactions


389
D
surface
−1
18
alternate surface







interactions


391
G
Buried
0
0
alternate interactions in







pocket


392
C
Buried
0
7
alternate interactions in







pocket


392
F
Buried
0
7
alternate interactions in







pocket


392
M
Buried
0
7
alternate interactions in







pocket


392
S
Buried
0
7
alternate interactions in







pocket


392
V
Buried
0
7
alternate interactions in







pocket


392
Y
Buried
0
7
alternate interactions in







pocket


409
T
surface
0
14
alternate interactions in




loop


pocket


437
L
Buried
0
7
alternate interactions in







pocket


437
M
Buried
0
7
alternate interactions in







pocket


443
G
Substrate
0
4
affect shape of loop




Capture




Loops


443
Q
Substrate
0
4
affect shape of loop




Capture




Loops


443
R
Substrate
1
4
affect shape of loop




Capture




Loops


443
S
Substrate
0
4
affect shape of loop




Capture




Loops


444
D
Substrate
−1
56
affect shape of loop




Capture




Loops


444
E
Substrate
−1
56
affect shape of loop




Capture




Loops


447
T
Substrate
0
23
alternate interactions in




Capture


pocket




Loops


447
V
Substrate
0
23
alternate interactions in




Capture


pocket




Loops


448
V
Substrate
0
77
alternate surface




Capture


interactions




Loops


460
A
Buried
0
21
alternate interactions in







pocket


461
A
Buried
−1
32
alternate interactions in







pocket


481
Y
Buried
0
0
alternate interactions in







pocket


488
L
Buried
1
13
alternate interactions in







pocket


502
F
Buried
0
1
alternate interactions in







pocket


502
M
Buried
0
1
alternate interactions in







pocket









Example 8
Growth Assay on 1024 Variants Selected from the Primary Screen
The primary in vitro specific activity screen identified variants of MEA P. alba that enhance the ability of the enzyme to catalyze the conversion DMAPP to isoprene. Since IspS must function inside a living cell, it was additionally necessary to measure the capability of the enzyme to convert DMAPP to isoprene in vivo. Examples 1 and 2 describe the methodology in determining the in vivo effectiveness of IspS. Essentially, by conversion of DMAPP to isoprene, IspS relieves the toxic effect of DMAPP on the growth of E. coli. Increased performance in comparison to wild type during the course of a growth curve indicates improved isoprene synthase function within a given strain. IspS variants that display both improved specific activity and the best growth performance are indicative of the enzymes best suited to improved isoprene production during fermentation.
Methods
Growth Assay and Specific Activity Measurements
1024 variants from the primary specific activity screen were selected for growth study and to confirm increased specific activity increases relative to the wild type MEA P. alba enzyme. Variants at positions that displayed high mutability (tolerance to mutation), and increased performance for both specific activity and expression not significantly less than wild type, were chosen for this study. Individual variants were isolated from their original glycerol stock plates and re-arrayed for the growth assay. Variants were induced at both low and high levels of IPTG, and their growth curves were determined in the presence of mevalonic acid (MVA). In these strains, MVA is taken up and drives flux through the mevalonic acid pathway to DMAPP, which is toxic to cell growth. Expression of functional P. alba IspS molecules allows for the conversion of DMAPP to isoprene, and the relief of growth inhibition. In these assays, better performing IspS molecules more effectively convert DMAPP to isoprene and result in improved growth.
Glycerol stocks of MEA P. alba IspS libraries were thawed briefly and inoculated into microtiter plates containing liquid LB with kanamycin at a concentration of 20 μg/ml. Cultures were grown overnight at 250 rpm, 30° C. to saturation in a shaking incubator. The next day, cultures were removed and inoculated at a ratio of 1:10 into TM3-glucose medium containing 50 ug/ml kanamycin and 40 or 100 uM IPTG (Sigma). Wild type controls were grown separately and inoculated into each microtiter plate containing TM3-glucose with a titration of IPTG concentrations, from 30 uM to 65 μM (for cultures induced at 40 μM), or 40 to 200 μM (for cultures induced at 100 μM), in separate wells. Plates were returned to the shaking incubator at 250 rpm, 30° C. and pre-induced for two hours. Cultures were then diluted into TM3-glucose medium containing 50 μg/ml kanamycin, 40 or 100 μM IPTG, and 20 mM MVA at a ratio of 1:10 in microtiter plates (Matrical). WT controls with or without MVA were included, as well as appropriate controls with titrations of IPTG. Plates were transferred to a Growth Profiler 1152 (Enzyscreen) and growth curves and optical densities (ODs) were determined according to the manufacturer's recommendation over a ten-hour time course. Performance indices (PIs) for growth of each strain were determined by comparison to four replicate WT strains induced at either 40 or 100 μM IPTG. PI values for OD at 300 minutes, Max OD, and area under the curve were calculated. Specific activity for all variants in this study, induced at the 40 μM IPTG level, was also determined according to the methods described in the previous example. Samples were isolated from the same pre-induction plate as the samples used in the growth assays.
Results
Table 16 provides definitions for locations of the residues listed in Tables 19 through 23. Table 19 lists all variants that displayed a PI value for specific activity greater than 1.4. Locations, surface accessibilities, and putative functions are also listed. Table 19 lists several variants that enhance the enzymatic efficiency of IspS, either alone or in combination. Locations of positions where variants displayed improved specific activity are shown in FIGS. 38 and 39. Variants with improved specific activity may allow for more efficient conversion of DMAPP to isoprene, and allow for improved cellular production of isoprene during fermentation.
Tables 20 and 21 list variants with improved growth at the 40 uM and 100 uM induction level, respectively. While several different growth parameters were measured, all correlated well with each other, so only PI values for maximum OD (OD Max) were examined for variants listed in Tables 20 and 21. Listed variants displayed OD Max values that were 50% better than WT (PI of 1.5 or greater) at the given induction level. Variants that displayed improved growth (a PI value greater than 1.3 for OD Max) in both 40 uM and 100 uM IPTG induction conditions are listed in Table 22, and shown in FIGS. 40 and 41. These variants represent mutations that may allow for the highest overall growth performance and conversion of DMAPP to isoprene in cells expressing IspS. Several of these variants map near or within a particular N-terminal helix region of MEA P. alba, spanning residues 134 to 179. Several changes at or near this location (“N-term helices” in Tables 16, 20 through 23) displayed a growth benefit at either or both growth conditions. Not only do multiple variants map to this location in MEA P. alba, but the variants that displayed the largest benefit to growth are facing outwards from the helix and are located on the surface of the enzyme (see FIGS. 41, 43, and 44).
Table 23 lists variants that displayed improved performance (PI greater than 1.2) for all three parameters of specific activity, OD Max at 40 uM, and 100 uM IPTG. Several of these variants are listed in Table 22, and the majority of them are also located in or near the N-terminal helix described above, with the exceptions of V30K and V84T (see FIG. 42). This indicates that alterations at the helix spanning residues 150 to 172 are critical not only for improved growth of the host cell, but also for improved enzymatic activity. Since there is no obvious catalytic role for the N-terminal helix (see FIG. 43), these variants may influence IspS activity either intra-molecularly through conformational change of the enzyme structure, or inter-molecularly via the above-mentioned interactions with an unidentified enzyme, cellular process or structure. MEA P. alba enzymes harboring variants at this particular location, either alone or in combination with other variants conferring beneficial properties such as improved catalytic rates, likely will allow for improved growth rate of host strains, and improved isoprene production during fermentation.







TABLE 19







Retested variants of MEA P. alba that displayed


PI values for specific activity > 1.4.















Charge
% Surface



Position
Mutation
Location
Change
Accessibility
Function















18
E
N-term
−1
64
alternate interactions







with nearby residues


18
D
N-term
−1
64
alternate interactions







with nearby residues


18
S
N-term
0
64
alternate interactions







with nearby residues


36
P
N-term
−1
33
alternate interactions







with nearby residues


82
Q
N-term
−1
27
alternate interactions







with nearby residues


87
S
surface
0
35
alternate surface




loop


interactions


87
N
surface
0
35
alternate surface




loop


interactions


87
R
surface
1
35
alternate surface




loop


interactions


137
C
N-term
0
15
alternate interactions in




helices


pocket


143
N
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


163
I
N-term
0
10
alternate interactions in




helices


pocket


163
Q
N-term
0
10
alternate interactions in




helices


pocket


170
G
N-term
1
79
alternate surface




helices


interactions


242
T
Dimer
−1
35
improve dimer interface




interface


interactions


251
E
surface
−1
74
alternate interactions







with nearby residues


409
S
surface
0
14
alternate interactions in




loop


pocket


437
M
Buried
0
7
alternate interactions in







pocket


437
K
Buried
1
7
alternate interactions in







pocket


443
G
Substrate
0
4
affect shape of loop




Capture




Loops


443
S
Substrate
0
4
affect shape of loop




Capture




Loops


443
Q
Substrate
0
4
affect shape of loop




Capture




Loops


444
p
Substrate
0
56
affect shape of loop




Capture




Loops


447
Q
Substrate
0
23
alternate interactions in




Capture


pocket




Loops


447
T
Substrate
0
23
alternate interactions in




Capture


pocket




Loops


447
M
Substrate
0
23
alternate interactions in




Capture


pocket




Loops


447
E
Substrate
−1
23
alternate interactions in




Capture


pocket




Loops


447
S
Substrate
0
23
alternate interactions in




Capture


pocket




Loops


447
R
Substrate
1
23
alternate interactions in




Capture


pocket




Loops


460
Q
Buried
0
21
alternate interactions in







pocket


460
S
Buried
0
21
alternate interactions in







pocket


460
G
Buried
0
21
alternate interactions in







pocket


460
A
Buried
0
21
alternate interactions in







pocket


461
D
Buried
−2
32
alternate interactions in







pocket


461
S
Buried
−1
32
alternate interactions in







pocket


461
T
Buried
−1
32
alternate interactions in







pocket


461
E
Buried
−2
32
alternate interactions in







pocket


542
N
surface
0
17
alternate interactions




loop


with nearby residues
















TABLE 20







Variants of MEA P. alba that displayed


PI values > 1.5 for OD Max at 40 mM IPTG















Charge
% Surface



Position
Mutation
Location
Change
Accessibility
Function















134
P
N-term
−1
37
loop stabilization




helices


138
C
N-term
−1
53
alternate interctions




helices


with nearby residues


143
F
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


143
V
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


156
G
N-term
0
13
allow more flexibility




helices


in loop


159
G
N-term
1
32
alternate interctions




helices


with nearby residues


159
Q
N-term
1
32
alternate interctions




helices


with nearby residues


163
C
N-term
0
10
alternate interctions




helices


with nearby residues


163
E
N-term
−1
10
alternate interctions




helices


with nearby residues


163
Q
N-term
0
10
alternate interctions




helices


with nearby residues


163
V
N-term
0
10
alternate interctions




helices


with nearby residues


163
Y
N-term
0
10
alternate interctions




helices


with nearby residues


166
C
N-term
0
46
alternate surface




helices


interactions


166
D
N-term
−1
46
alternate surface




helices


interactions


166
G
N-term
0
46
alternate surface




helices


interactions


166
P
N-term
0
46
alternate surface




helices


interactions


166
V
N-term
0
46
alternate surface




helices


interactions


167
M
N-term
0
21
alternate interctions




helices


with nearby residues


170
G
N-term
1
79
alternate surface




helices


interactions


170
H
N-term
1
79
alternate surface




helices


interactions


170
K
N-term
2
79
alternate surface




helices


interactions


170
N
N-term
1
79
alternate surface




helices


interactions


170
R
N-term
2
79
alternate surface




helices


interactions


170
S
N-term
1
79
alternate surface




helices


interactions


170
W
N-term
1
79
alternate surface




helices


interactions


414
F
surface
−1
70
alternate surface




loop


interactions


414
G
surface
−1
70
alternate surface




loop


interactions


414
N
surface
−1
70
alternate surface




loop


interactions


414
P
surface
−1
70
alternate surface




loop


interactions


421
R
surface
1
23
alternate surface




loop


interactions


491
Q
surface
0
58
alternate surface




loop


interactions


491
V
surface
0
58
alternate surface




loop


interactions


491
Y
surface
0
58
alternate surface




loop


interactions
















TABLE 21







Variants of MEA P. alba that displayed


PI values > 1.5 for OD Max at 100 mM IPTG















Charge
% Surface



Position
Mutation
Location
Change
Accessibility
Function















29
N
N-term
1
34
alternate surface







interactions


47
V
surface
0
16
affect shape of loop




loop


86
C
surface
0
59
alternate interactions




loop


94
A
surface
−1
73
affect shape of loop




loop


131
F
N-term
1
58
improved interaction




helices


with neighboring his


134
E
N-term
−2
37
loop stabilization




helices


134
P
N-term
−1
37
loop stabilization




helices


156
G
N-term
0
13
allow more flexibility




helices


in loop


162
P
N-term
0
60
loop stabilization




helices


169
C
N-term
−1
60
alternate surface




helices


interactions


178
E
N-term
−2
73
alternate surface




helices


interactions


179
T
N-term
1
46
alternate surface




helices


interactions


231
D
hinge
−1
33
alternate surface




region


interactions


231
K
hinge
1
33
alternate surface




region


interactions


231
R
hinge
1
33
alternate surface




region


interactions


231
T
hinge
0
33
alternate surface




region


interactions


231
V
hinge
0
33
alternate surface




region


interactions


242
N
dimer
−1
35
improve dimer interface




interface


interactions


242
I
dimer
−1
35
improve dimer interface




interface


interactions


369
C
active
0
2
alter active site cavity




site


414
C
surface
−1
70
alternate surface




loop


interactions


414
F
surface
−1
70
alternate surface




loop


interactions


414
G
surface
−1
70
alternate surface




loop


interactions


414
N
surface
−1
70
alternate surface




loop


interactions


421
D
surface
−1
23
alternate surface




loop


interactions
















TABLE 22







Variants of MEA P. alba that displayed PI


values > 1.3 for OD Max at 40 and 100 mM IPTG















Charge
% Surface



Position
Mutation
Location
Change
Accessibility
Function















50
S
N-term
−1
86
alternate surface







interactions


81
F
N-term
1
48
alternate surface







interactions


134
E
N-term
−2
37
improved interaction




helices


with neighboring his


134
P
N-term
−1
37
loop stabilization




helices


137
N
N-term
0
15
alternate interactions in




helices


pocket


143
V
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


156
G
N-term
0
13
allow more flexibility




helices


in loop


159
D
N-term
0
32
alternate interactions




helices


with nearby residues


159
G
N-term
1
32
allow more flexibility




helices


in loop


159
Q
N-term
1
32
alternate interactions




helices


with nearby residues


166
C
N-term
0
46
alternate surface




helices


interactions


166
W
N-term
0
46
alternate surface




helices


interactions


167
M
N-term
0
21
alternate interactions




helices


with nearby residues


167
N
N-term
0
21
alternate interactions




helices


with nearby residues


169
C
N-term
−1
60
alternate surface




helices


interactions


170
H
N-term
1
79
alternate surface




helices


interactions


170
K
N-term
2
79
alternate surface




helices


interactions


170
W
N-term
1
79
alternate surface




helices


interactions


414
C
surface
−1
70
alternate surface




loop


interactions


414
F
surface
−1
70
alternate surface




loop


interactions


414
G
surface
−1
70
alternate surface




loop


interactions


414
N
surface
−1
70
alternate surface




loop


interactions


414
P
surface
−1
70
alternate surface




loop


interactions
















TABLE 23







Variants of MEA P. alba that displayed PI values > 1.2 for Specific


Activity, OD Max at 40 μM IPTG, and 100 μM IPTG















Charge
% Surface



Position
Mutation
Location
Change
Accessibility
Function















30
K
N-term
1
60
replace surface







hydrophobic w/charged


84
T
N-term
0
30
polar residue for better







solvent interactions


134
C
N-term
−1
37
size reduction,




helices


improved interaction







with neighboring His


134
D
N-term
−2
37
improved interaction




helices


with neighboring his


134
E
N-term
−2
37
improved interaction




helices


with neighboring his


140
S
N-term
0
1
may improve




helices


interactions with other







polar residues in pocket


140
T
N-term
0
1
may improve




helices


interactions with other







polar residues in pocket


143
F
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


143
I
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


143
M
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


143
V
N-term
0
1
alternate interactions in




helices


hydrophobic pocket


163
I
N-term
0
10
alternate interactions in




helices


pocket


163
M
N-term
0
10
alternate interactions in




helices


pocket


166
P
N-term
0
45
stabilize helix




helices


166
V
N-term
0
45
alternate surface




helices


interactions


169
Q
N-term
−1
60
alternate surface




helices


interactions


170
H
N-term
1
80
alternate surface




helices


interactions


170
K
N-term
2
80
alternate surface




helices


interactions


172
V
N-term
0
47
affect shape of loop




helices









Example 9
Specific Activity and Growth Assays on Combinatorial Libraries
Single variants of MEA P. alba IspS improved for specific activity, growth, or both traits were selected for combination into three seven-member libraries.
Methods
Libraries were constructed in the pCL201 vector and transformed into the MD09-170 screening strain (DNA2.0). 160 individual variants, representing approximately 80 to 90% of the 128 possible combinations in each library, were screened for both specific activity and growth following the methods described in the previous examples. Table 24 lists the variants chosen for combinatorial libraries, their locations in the crystal structure, surface accessibilities, and selection criteria (either specific activity, growth or both). Putative functions for the amino acids at these positions were listed in Examples 7 and 8.







TABLE 24







Variants chosen for combinatorial libraries


















% Surface



Residue
Position
Mutation
Library
Location
Accessibility
Selection Criteria
















S
288
C
1/2
C-term
0
Growth (Solubility)


S
22
R
1
N-term
54
Specific Activity


R
71
I
1
N-term
3
Specific Activity


S
444
D
1/2
SubLoop
56
Specific Activity


M
460
A
1
SubLoop
21
Specific Activity


A
443
G
1
SubLoop
4
Specific Activity


T
502
M
1
Buried
1
Specific Activity


V
409
T
2
surface
14
Growth/Specific Activity






loop


R
242
N
2
DimInt
35
Growth/Specific Activity


K
414
F
2
surface
70
Growth






loop


V
162
P
2
N-term
60
Growth






helix


G
87
R
2
surface
35
Growth/Specific Activity






loop


S
288
T
3
C-term
0
Growth/Specific Activity


N
47
V
3
surface
16
Growth






loop


I
447
T
3
SubLoop
23
Specific Activity


E
170
H
3
N-term
79
Growth






helix


S
231
T
3
hinge
33
Growth






region


K
414
N
3
surface
70
Growth






loop


I
156
G
3
N-term
13
Growth






helix









Results

Combinatorial variants displaying significantly improved specific activity and/or growth performance were identified. Table 25 contains a list of combinatorial variants that displayed performance index (PI) values for specific activity greater than 2.6. The left hand column lists the variant number, and the subsequent columns list the genotype for the 7 different positions in that library. Variants with improved specific activity allow for more efficient enzymatic conversion of DMAPP to isoprene, likely by improved kinetic parameters. Table 26 contains a list of combinatorial variants that displayed PI values greater than 1.3 for specific activity, OD Max at 40 uM, and OD Max at 100 uM. IspS variants improved for both specific activity and growth parameters also convert DMAPP to isoprene more efficiently than the WT enzyme, and likely are beneficial to growth of the host strain by mediation of a deleterious effect of IspS within the host.
Since each library member contained any combination of seven possible mutations, effects of variants were observed multiple times in different configurations. This provided a robust internal control to help identify the most effective combinations present in each library. For example, the S444D mutation was present in the highest specific activity combinatorial variants, suggesting that this variant, in combination with other improved variants, is particularly beneficial for in vitro activity. M460A, A443G, and I447T also displayed this type of effect. Similarly, the V162P mutation was present in all combinatorial variants with improved properties for both specific activity and growth, suggesting that V162P works well in combination with other variants and may be an ideal mutation for more efficient conversion of DMAPP to isoprene within the host cell, for the reasons described in the previous paragraph. I156G and E170H also showed this effect. The variants G087R, R242N, and S288T also displayed improved specific activity in combination with other variants, but were not always among the highest performers for the in vitro assay. The combinatorial variants listed in Tables 25 and 26 may represent significantly improved IspS enzymes that allow for optimal conversion of DMAPP into isoprene during fermentation of host cells. The presence or absence of individual mutations in these particular combinatorial variants additionally may signify the best overall mutations that can be combined in future variants of IspS, critical to the optimization of isoprene production by fermentation of microorganisms.







TABLE 25







Combinatorial variants of MEA P. alba displaying


PI specific activity values >2.6.














Variant
Pos 1
Pos 2
Pos 3
Pos 4
Pos 5
Pos 6
Pos 7

















1
022R
071I
288C
443A
444D
460A
502M


2
022R
071I
288C
443G
444D
460A
502M


3
022S
071I
288C
443A
444S
460A
502T


4
022S
071R
288C
443A
444D
460A
502T


5
087G
162P
242N
288S
409T
414F
444D


6
087G
162P
242N
288S
409T
414K
444D


7
087G
162P
242R
288C
409T
414F
444D


8
087G
162V
242N
288C
409V
414F
444D


9
087G
162V
242R
288C
409T
414F
444D


10
087G
162V
242R
288C
409V
414K
444D


11
087R
162P
242N
288C
409T
414F
444D


12
087R
162P
242N
288C
409T
414K
444D


13
087R
162P
242N
288S
409T
414K
444D


14
087R
162P
242N
288S
409V
414F
444D


15
087R
162P
242N
288S
409V
414K
444D


16
087R
162P
242R
288C
409T
414F
444D


17
087R
162P
242R
288C
409V
414F
444D


18
087R
162P
242R
288C
409V
414K
444D


19
087R
162P
242R
288S
409T
414F
444D


20
087R
162P
242R
288S
409V
414K
444D


21
087R
162V
242N
288C
409V
414F
444D


22
087R
162V
242N
288S
409T
414F
444D


23
087R
162V
242N
288S
409T
414K
444D


24
087R
162V
242N
288S
409V
414F
444D


25
087R
162V
242R
288C
409T
414K
444D


26
087R
162V
242R
288C
409V
414K
444D


27
087R
162V
242R
288S
409V
414F
444D
















TABLE 26







Combinatorial variants of MEA P. alba displaying


PI >1.3 for specific activity, OD Max at 40 μM IPTG,


and OD Max at 100 μM IPTG.














Variant
Pos 1
Pos 2
Pos 3
Pos 4
Pos 5
Pos 6
Pos 7

















1
047N
156G
170H
231S
288T
414K
447I


2
047V
156G
170H
231S
288T
414K
447I


3
047V
156I
170H
231S
288T
414F
447I


4
047V
156I
170H
231T
288T
414K
447I


5
087G
162P
242N
288C
409T
414F
444S


6
087G
162P
242N
288C
409V
414K
444S


7
087G
162P
242N
288S
409T
414F
444S


8
087G
162P
242R
288C
409T
414K
444S


9
087G
162P
242R
288S
409T
414K
444S


10
087R
162P
242N
288C
409T
414F
444S


11
087R
162P
242N
288C
409V
414K
444S


12
087R
162P
242R
288C
409V
414K
444S









Example 10
Specific Activity Determination of N-Terminal Truncations of MEA P. alba IspS 
Isoprene synthase contains tandem arginine residues at the N-terminus that are necessary for proper enzymatic conversion of DMAPP to isoprene. As a truncated variant, MEA P. alba demonstrates high specific activity in comparison to enzymes with longer N-terminal regions, up to the naturally occurring chloroplast targeting peptide. The MEA P. alba enzyme has only two residues upstream of the tandem arginine residues (see FIG. 45), yet the function of these residues with regard to enzyme activity was not reported. N-terminal truncations of the MEA P. alba enzyme therefore were generated and assayed to determine if further truncations confer a specific activity benefit to IspS.
Methods
The two truncations of MEA P. alba were constructed by QuikChange (Stratagene) PCR on the template pCL201 (see Table 27 for primer sequences) as previously described following the manufacturer's recommended protocol. The PCR product was treated with 1 μl DpnI (Roche) for 3 hours, and then 1 μl of the entire reaction was transformed into chemically competent E. coli Top10 cells (Invitrogen) according to the manufacturer's recommended protocol. Cells were recovered and plated on LB medium containing 50 μg/ml kanamycin. The next day, positive colonies were chosen for growth, plasmid purification (Qiagen) and sequencing (Quintara Biosciences). Plasmids which harbored the correct truncations were selected for sequencing of the entire open reading frame to confirm the integrity of the coding sequence. These plasmids, pDW207 (see FIG. 46) and pDW208 (see FIG. 47), were transformed by electroporation into the expression strain MD09-170 for determination of specific activity (see Table 28). Specific activity was determined as previously described. At least 30 replicates of each truncation were analyzed in comparison to MEA P. alba.







TABLE 27





Primers used for QuikChange Mutagenesis
















HgS MRR Forward
TATACATATGCGTCGCTCTGCGAACTACGA



(SEQ ID NO: 30)


 


HgS MRR Reverse
CAGAGCGACGCATATGTATATCTCCTTCTT



(SEQ ID NO: 31)


 


HgS MAR Forward
TATACATATGGCACGTCGCTCTGCGAACTA



(SEQ ID NO: 32)


 


HgS MAR Reverse
AGCGACGTGCCATATGTATATCTCCTTCTT



(SEQ ID NO: 33)
















TABLE 28







Strains with N-terminal truncations











Strain
Plasmid
Description







DW618
pDW207
BL21 (DE3) PL.2-mKKDyI + P. alba





IspS MAR (−1 from MEA P. alba)



DW619
pDW208
BL21 (DE3) PL.2-mKKDyI + P. alba





IspS MRR (−2 from MEA P. alba)










Results

Specific activities of the truncated molecules of P. alba IspS expressed in strains DW618 (MAR) or DW619 (MRR) were either not improved or slightly lower, respectively, than the parental MEA P. alba enzyme. Table 29 shows performance index values for both the MAR and MRR truncations of P. alba IspS. The MAR truncation displayed specific activity that was approximately equivalent to the control MEA P. alba molecule, and the MRR truncation displayed specific activity that was approximately 81% of the control. Although these truncations did not have increased specific activity in comparison to MEA P. alba, they retained sufficient activity to be of potential future use in fermentation strains that convert DMAPP to isoprene via an IspS enzyme, where complete removal of the N-terminus up to but not including the tandem arginine residues is required.







TABLE 29





Performance index values for truncated variants of P. alba IspS




















Strain
Variant
PI Specific Activity
Standard Deviation






DW618
MAR
0.983189
0.091889


 



DW619
MRR
0.813857
0.072938










Amino Acid Sequence of P. alba IspS MAR (SEQ ID NO: 34)


MARRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFLTLLELI


 


DNVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQHGFEVSQEAF


 


SGFKDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKIGKEL


 


AEQVNHALELPLHRRTQRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLR


 


ETSRWWRRVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFSFVTIIDDIY


 


DVYGTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDKGENILP


 


YLTKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIK


 


KEEIENLQKYHDTISRPSHIFRLCNDLASASAEIARGETANSVSCYMRTKGISEELATESV


 


MNLIDETWKKMNKEKLGGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVL


 


SVITEPILPFER


 


DNA Sequence of plasmid pDW207 (SEQ ID NO: 35)


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccc


 


tagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttcc


 


gatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccc


 


tttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggat


 


tttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggca


 


cttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcg


 


agcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgag


 


gcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa


 


ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggcca


 


gccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgtta


 


aaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttc


 


taatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagag


 


gcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcat


 


cgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaa


 


tttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatga


 


ccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaa


 


tctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctt


 


cagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgc


 


tctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgca


 


gcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctat


 


gagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggga


 


gcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggggg


 


cggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccct


 


gattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgag


 


gaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctct


 


gatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgc


 


gccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatc


 


accgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcg


 


ttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaag


 


ggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactg


 


gaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaataca


 


gatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagac


 


tttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcg


 


gtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggcc


 


gccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccg


 


aataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtc


 


ctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaag


 


gctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcggga


 


aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcacca


 


gtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcga


 


aaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgc


 


agcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagc


 


atttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccag


 


ccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccac


 


gcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgc


 


aggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgc


 


cgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaa


 


tttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgg


 


gaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtct


 


gataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgc


 


cataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagta


 


ggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgcca


 


ccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagca


 


accgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcacta


 


taggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatggcacgtcgctctgcgaact


 


acgaacctaacagctgggactatgattacctgctgtcctccgacacggacgagtccatcgaagtatacaaagacaaagcgaaaaagctgg


 


aagccgaagttcgtcgcgagattaataacgaaaaagcagaatttctgaccctgctggaactgattgacaacgtccagcgcctgggcctggg


 


ttaccgtttcgagtctgatatccgtggtgcgctggatcgcttcgtttcctccggcggcttcgatgcggtaaccaagacttccctgcacggtacg


 


gcactgtctttccgtctgctgcgtcaacacggttttgaggtttctcaggaagcgttcagcggcttcaaagaccaaaacggcaacttcctggag


 


aacctgaaggaagatatcaaagctatcctgagcctgtacgaggccagcttcctggctctggaaggcgaaaacatcctggacgaggcgaag


 


gttttcgcaatctctcatctgaaagaactgtctgaagaaaagatcggtaaagagctggcagaacaggtgaaccatgcactggaactgccact


 


gcatcgccgtactcagcgtctggaagcagtatggtctatcgaggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagctggc


 


aattctggattacaacatgatccagtctgtataccagcgtgatctgcgtgaaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaact


 


gcactttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagcattcgaaccgcaatactccgactgccgtaactccgtcgc


 


aaaaatgttttctttcgtaaccattatcgacgatatctacgatgtatacggcaccctggacgaactggagctgtttactgatgcagttgagcgttg


 


ggacgtaaacgccatcaacgacctgccggattacatgaaactgtgctttctggctctgtataacactattaacgaaatcgcctacgacaacct


 


gaaagataaaggtgagaacatcctgccgtatctgaccaaagcctgggctgacctgtgcaacgctttcctgcaagaagccaagtggctgtac


 


aacaaatctactccgacctttgacgactacttcggcaacgcatggaaatcctcttctggcccgctgcaactggtgttcgcttacttcgctgtcgt


 


gcagaacattaaaaaggaagagatcgaaaacctgcaaaaataccatgacaccatctctcgtccttcccatatcttccgtctgtgcaatgacct


 


ggctagcgcgtctgcggaaattgcgcgtggtgaaaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaagaactggcta


 


ccgaaagcgtgatgaatctgatcgatgaaacctggaaaaagatgaacaaggaaaaactgggtggtagcctgttcgcgaaaccgttcgtgg


 


aaaccgcgatcaacctggcacgtcaatctcactgcacttatcataacggcgacgcgcatacctctccggatgagctgacccgcaaacgcgt


 


tctgtctgtaatcactgaaccgattctgccgtttgaacgctaaggatccgaattcgagctccgtcgacaagcttgcggccgcactcgagcacc


 


accaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataac


 


cccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat


 


Amino Acid Sequence of P. alba IspS MRR (SEQ ID NO: 36)


MRRSANYEPNSWDYDYLLSSDTDESIEVYKDKAKKLEAEVRREINNEKAEFLTLLELID


 


NVQRLGLGYRFESDIRGALDRFVSSGGFDAVTKTSLHGTALSFRLLRQHGFEVSQEAFS


 


GFKDQNGNFLENLKEDIKAILSLYEASFLALEGENILDEAKVFAISHLKELSEEKIGKELA


 


EQVNHALELPLHRRTQRLEAVWSIEAYRKKEDANQVLLELAILDYNMIQSVYQRDLRET


 


SRWWRRVGLATKLHFARDRLIESFYWAVGVAFEPQYSDCRNSVAKMFSFVTIIDDIYDV


 


YGTLDELELFTDAVERWDVNAINDLPDYMKLCFLALYNTINEIAYDNLKDKGENILPYL


 


TKAWADLCNAFLQEAKWLYNKSTPTFDDYFGNAWKSSSGPLQLVFAYFAVVQNIKKE


 


EIENLQKYHDTISRPSHIFRLCNDLASASAEIARGETANSVSCYMRTKGISEELATESVMN


 


LIDETWKKMNKEKLGGSLFAKPFVETAINLARQSHCTYHNGDAHTSPDELTRKRVLSVI


 


TEPILPFER


 


Sequence of plasmid pDW208 (SEQ ID NO: 37)


tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccc


 


tagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttcc


 


gatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccc


 


tttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggat


 


tttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggca


 


cttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattcttagaaaaactcatcg


 


agcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgag


 


gcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataa


 


ggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggcca


 


gccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgtta


 


aaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttc


 


taatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagag


 


gcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcat


 


cgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaa


 


tttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatga


 


ccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaa


 


tctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctt


 


cagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgc


 


tctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgca


 


gcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctat


 


gagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggga


 


gcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagggggg


 


cggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccct


 


gattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgag


 


gaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctct


 


gatgccgcatagttaagccagtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgc


 


gccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatc


 


accgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcg


 


ttgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaag


 


ggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactg


 


gaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaataca


 


gatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagac


 


tttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcg


 


gtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggcc


 


gccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccg


 


aataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtc


 


ctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaag


 


gctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcggga


 


aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcacca


 


gtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcga


 


aaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgc


 


agcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagc


 


atttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccag


 


ccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccac


 


gcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgc


 


aggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgc


 


cgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaa


 


tttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgg


 


gaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtct


 


gataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgc


 


cataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagta


 


ggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgcca


 


ccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagca


 


accgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcacta


 


taggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatgcgtcgctctgcgaactacg


 


aacctaacagctgggactatgattacctgctgtcctccgacacggacgagtccatcgaagtatacaaagacaaagcgaaaaagctggaag


 


ccgaagttcgtcgcgagattaataacgaaaaagcagaatttctgaccctgctggaactgattgacaacgtccagcgcctgggcctgggttac


 


cgtttcgagtctgatatccgtggtgcgctggatcgcttcgtttcctccggcggcttcgatgcggtaaccaagacttccctgcacggtacggca


 


ctgtctttccgtctgctgcgtcaacacggttttgaggtttctcaggaagcgttcagcggcttcaaagaccaaaacggcaacttcctggagaac


 


ctgaaggaagatatcaaagctatcctgagcctgtacgaggccagcttcctggctctggaaggcgaaaacatcctggacgaggcgaaggttt


 


tcgcaatctctcatctgaaagaactgtctgaagaaaagatcggtaaagagctggcagaacaggtgaaccatgcactggaactgccactgca


 


tcgccgtactcagcgtctggaagcagtatggtctatcgaggcctaccgtaaaaaggaggacgcgaatcaggttctgctggagctggcaatt


 


ctggattacaacatgatccagtctgtataccagcgtgatctgcgtgaaacgtcccgttggtggcgtcgtgtgggtctggcgaccaaactgcac


 


tttgctcgtgaccgcctgattgagagcttctactgggccgtgggtgtagcattcgaaccgcaatactccgactgccgtaactccgtcgcaaaa


 


atgttttctttcgtaaccattatcgacgatatctacgatgtatacggcaccctggacgaactggagctgtttactgatgcagttgagcgttgggac


 


gtaaacgccatcaacgacctgccggattacatgaaactgtgctttctggctctgtataacactattaacgaaatcgcctacgacaacctgaaa


 


gataaaggtgagaacatcctgccgtatctgaccaaagcctgggctgacctgtgcaacgctttcctgcaagaagccaagtggctgtacaaca


 


aatctactccgacctttgacgactacttcggcaacgcatggaaatcctcttctggcccgctgcaactggtgttcgcttacttcgctgtcgtgcag


 


aacattaaaaaggaagagatcgaaaacctgcaaaaataccatgacaccatctctcgtccttcccatatcttccgtctgtgcaatgacctggcta


 


gcgcgtctgcggaaattgcgcgtggtgaaaccgcaaatagcgtttcttgttacatgcgcactaaaggtatctccgaagaactggctaccgaa


 


agcgtgatgaatctgatcgatgaaacctggaaaaagatgaacaaggaaaaactgggtggtagcctgttcgcgaaaccgttcgtggaaacc


 


gcgatcaacctggcacgtcaatctcactgcacttatcataacggcgacgcgcatacctctccggatgagctgacccgcaaacgcgttctgtc


 


tgtaatcactgaaccgattctgccgtttgaacgctaaggatccgaattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccac


 


caccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataacccctt


 


ggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat









Example 11
Productive Mutations, Combinable Mutations and Suitability Score
Productive positions are described as those positions within a molecule that are most useful for making combinatorial variants exhibiting an improved characteristic, where the position itself allows for at least one combinable mutation. Combinable mutations can be described as those substitutions in a molecule that can be used to make selected combinatorial variants. Combinable mutations do not significantly decrease expression, specific activity or growth, while at the same time improving at least one desired characteristic of the molecule such as growth or specific activity. Positions in IspS containing all combinable mutations were determined using performance index (PI) values resulting from the DMAPP assay for specific activity and protein determination, as described in Example 7. Productive positions are the positions which have shown a certain degree of tolerance for multiple substitutions, while at the same time meeting a set of criteria for combinability as set forth below.
When evaluating the data set, the most productive positions were determined when the following criteria were applied:
Positions containing substitutions where the minimum performance indices (PI) relative to wild type IspS for specific activity and expression are greater than or equal to a PI of 0.9 and where at least one PI relative to wild type IspS for specific activity or growth is greater than or equal to a PI of 1.0 (Group A).
Positions containing substitutions where the minimum performance indices (PI) relative to wild type IspS for specific activity and expression are greater than or equal to a PI of 0.8 and where at least one PI relative to wild type IspS for specific activity or growth is greater than or equal to a PI of 1.2 (Group B).
Positions containing substitutions where the minimum performance indices (PI) relative to wild type IspS for specific activity and expression are greater than or equal to a PI of 0.5 and where at least one PI relative to wild type IspS for specific activity or growth is greater than or equal to a PI of 1.5 (Group C).
Groups A, B, and C further contain positions that have differing degrees of tolerance for multiple substitutions. To measure this degree of substitutions tolerated, a Rank was assigned to each position. The Rank was assigned according to the percentage of the substitutions within each position that fall within groups A, B, or C. Combinable positions and substitutions are shown in Table 31.
The criteria to determine the Rank for productive positions are as follows:
Positions where less than 15% but greater than 0% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “1”.
Positions where less than 30%, but greater than, or equal to 15% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “2”.
Positions where less than 50%, but greater than, or equal to 30% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “3”.
Positions where greater than, or equal to 50% of the substitutions at a given position fall within groups A, B, or C are given a Rank of “4”.
Substitutions are further assigned a Suitability Score based on the group(s) the substitution is a member of, and where a higher score represents a substitution more suitable for use in making combinatorial variants. Suitability scores are represented and defined in Table 30. Suitability scores and Rank for individual substitutions of IspS that fit the above criteria are represented in Table 31.







TABLE 30







Suitability Score for the defined groups.










Substitutions
Suitability



Occur in Group(s):
Score







A, B and C
+++++



A and B
++++



A or (B and C)
+++



B
++



C
+

















TABLE 31







Suitability Score and Rank of positions in IspS with substitutions


within the positions that are combinable.















VARIANTS
VARIANTS
VARIANTS
VARIANTS
VARIANTS




SUITABILITY
SUITABILITY
SUITABILITY
SUITABILITY
SUITABILITY




SCORE
SCORE
SCORE (+++)
SCORE
SCORE


POSITION
RANK
(+)
(++)
WT AA 1ST
(++++)
(+++++)
















2
4
Q
FGLR
EHIS
CDNTV
AKP


 


3
2


AEGKNQRT




 


6
1


S
NT



 


13
2
N
M
SQT




 


17
1
T

DE




 


18
3
H

YFMN
AQR
DEKS


 


19
1


LF

Y


 


20
2


LIV
T



 


21
1
M

S

W


 


22
3
AHKNRTY

S
Q



 


23
2
K
EG
DT
N



 


24
2
AMS

T
C
LV


 


25
2

NQ
DAES
T



 


26
4
Y
L
EGNQT
DHKMRSV
C


 


27
4

RY
SEFKV
ACGHILMPQ
DN


 


28
3

CW
IEFMP
DN



 


29
2

G
EDPRT
Q
N


 


30
3


VNQ
ADEMRT



 


31
2
L
D
YQW
N



 


32
2

C
KDGNR
E



 


33
2
K
E
DN




 


34
2


KDEQS




 


36
4
HTWY
GS
KFR
ACDEMNPQ



 


37
4

WY
KFI
AEGHMNRT
CDPQS


 


40
3


ACDEFMNPQV




 


41
3
M

ECDFNQSV




 


42
3
C
L
VAST
FI
M


 


43
2

G
RIQ




 


44
2


RADKMY
NQ



 


45
2


ECMNQ




 


46
1


IFV




 


47
4
C
F
NEIKRV
AGHMQTW
DS


 


48
4


NACEFLQRS
HIK
DGT


 


49
3


EGHIRSW
AC
LV


 


50
4

IQV
KCGMNPR
ADEFHSY



 


51
2


AEGLQT




 


52
1

D
E




 


53
3

CGMS
FD
EHNPQV



 


54
2


LACEHIQ
M



 


55
3

PW
TAHNQSY
CDE



 


56
4


LHQRS
CN
EFGIKTVY


 


57
1


LI




 


58
3
ADFGHRSY
C
E
N



 


59
3

C
LFMSVY
HT



 


60
1


ICV




 


62
1


NV




 


63
1
C

V




 


68
1


L
I



 


69
1

AQ
G




 


71
2
HLV

RI
KM



 


73
1


ED




 


74
3
Q
AC
SGMP
DENY



 


75
1


DE




 


77
2


RANTV
L



 


78
4


GEIKNPQVW
ADFLM



 


79
3
C
LN
AMRY
QT



 


81
4

W
DCEHLN
AFGMRSTV
Q


 


82
4

D
RCFGLW
AEHIKMQS
NTVY


 


83
2


FGHILV
W



 


84
4


VFHLNQRSTWY
A
M


 


85
2

GM
SCLNR




 


86
2

GQ
SCN
ADM



 


87
4

HMQSV
GCEFKLNT
DP



 


88
2


GCDIVWY




 


89
2

HM
FCI




 


91
4

F
ACDEGHLRST
KW







VY




 


92
3

D
VACEFGILQW




 


93
4

M
THIQVW
ADEGLNPY
CFRS


 


94
4

FQ
KCVY
ADEHILMNR
GP







ST



 


95
4

R
TCHKM
AEPQSVY
DFGINW


 


96
2
CG
DNQ
S




 


97
2


LAMP
F



 


98
4

P
HCSTVW
ADFGILMNQ



 


99
4

DNS
GACHPQT
EFM



 


100
2

C
TAILMV




 


101
1


AS




 


102
1


LM




 


103
2

M
SACGL




 


107
2

A
LCF




 


109
2


QCNS
E



 


110
1

A
H




 


111
1


GA




 


113
2

MPQ
ECHV




 


114
2

AM
VC




 


115
2


SDY
A



 


116
4


QGHLSTV
ACDEIP



 


117
3

QW
EADI
CFLMV



 


118
2


AIV
M



 


119
1


FLM




 


120
4
IL

SADEFKNRWY
HTV
CGMQ


 


121
2

FM
GDLVW




 


123
2


KISWY
LT
V


 


124
1


DCE




 


125
4

CP
QADGHKLNST
EIY







VW




 


126
4
FR
GSY
N
ACDMTV
E


 


127
3
L
EMNS
GDFW
C



 


128
3

ELMST
NA
CDPQ



 


129
2

W
FLY




 


130
3


LACDQVY
E



 


131
4

N
EDFGR
ACPQSV
HKLMTWY


 


132
4

QS
N
CDFHLRWY
IP


 


133
3


LEGIPQTVY
D



 


134
4

CFP
KDGHILNRWY
EMQSTV
A


 


135
2
G

EHS




 


136
1


DN
E



 


137
4


IACDGPQSV
EHN
T


 


138
4
L
FMQT
KCDEPRSV
IN



 


139
3
DG

APSTV
N
CQ


 


140
3
C
A
INQSTV
MW



 


143
4

I
LAFGNRW
S
CDEHKMQT








VY


 


146
1


AM




 


149
1

W
L




 


150
1

GS
A




 


151
4


LEGMNQRSTVW
CHI
AF


 


152
2


EADIMP




 


153
1


GD
C



 


155
4


NEKM
ITVY
ACGHQRSW


 


156
4
V
ACGMQS
IEKLRY
DNT



 


158
1


DE




 


159
2
D
C
E
M



 


160
2

GY
AFHS
I



 


161
3
M

KLRSY
ACNQ



 


162
3

GH
VDFNPT
S



 


163
3


FCHIMVWY
EQ



 


164
2
C
S
A
T



 


165
1

D
I




 


166
4

Y
SCEHKPQVW
ADG
N


 


167
4

Y
HCLP
AEGKMRSTW
FINQV


 


169
4

P
KEGR
DIMST
ACHNQV


 


170
4


EGINR
HKMQTV
LSWY


 


171
4


LCEGIMW
HKRS
ANQTVY


 


172
3
V
HKT
SGNQR
AC



 


173
1

Q
E




 


175
4


KAGHNPTV
S
CFIQR


 


176
2


IACNQV
M



 


177
3
S

GDEHNPT
AC



 


178
4

C
KDEGILMNPQ
AFRST







VY




 


179
4


EGIPQSTVWY
ACLMN
D


 


180
4
KMR
E
LFHVW
CQT
AI


 


181
4
GIK
CTY
AFMNW
HQSV



 


182
2


EHN
S



 


183
2

M
QAL




 


185
1

A
ND




 


187
1


ACS




 


188
1

M
L




 


190
2
C

L
IM



 


193
1

Q
HW




 


194
2
HWY

RI
L



 


196
1


TV




 


197
3
AEHIPT

QG
S
CDN


 


203
1

F
W




 


204
2


SAFMWY
C



 


208
1

A
Y




 


210
1


KM




 


211
4
L
C
KDEFGHIMRSTV
ANQ



 


212
2


EADMPQT




 


215
2

W
NDY
CH



 


216
2


QAEN




 


217
3
AM
D
VCEKNPQT
I



 


218
1
M

LV




 


219
2
A

LIMV
C



 


220
1


EDN




 


221
1


L
M



 


222
1


AS




 


223
1


IC




 


224
2


LACTV




 


226
1


YF




 


228
2
L

MHR
FY



 


229
2


IA
V
C


 


231
4
M
CI
SDGHRV
KQT
A


 


232
1


VQ
I



 


234
1

R
Q




 


235
2

E
RADN
K



 


238
1
K

R




 


240
2
ILM

TV

C


 


241
2
L

SC
AMT



 


242
4


RKL
ADEHIMNQST
G


 


245
4
AEGN

R
IL
CKMQTV


 


246
2

E
RHQ
DK
N


 


247
2
I

V
T
LM


 


249
1
I

L




 


251
3


TH
AGKR
DENPQS


 


253
1


LM




 


254
2
R
CET
H
D



 


260
1


LM




 


268
1


VI




 


270
1


VI




 


271
2


AS
CV
T


 


272
2
HS

FQ
DGPW



 


275
1


QE




 


276
2

M
YFHQ




 


278
4

FHIK
DLMRY
AENQSTVW



 


279
1


C
A



 


282
3
HIW
R
SC
AQ
Y


 


296
1
M

I
V



 


297
1

F
Y




 


302
1


T
H



 


306
1


L

C


 


307
1


EQR




 


308
1

S
L




 


314
1

F
EH




 


315
2
M

RGK




 


317
2
C

DS
EQ
N


 


319
1


N
F
M


 


320
2


ANT
C



 


321
1


ILM




 


322
1

D
N




 


323
1


DIT




 


327
2
NQ

Y
M



 


328
1


ML




 


329
2
A

KGQR




 


331
1


CT
P



 


332
1


FY




 


333
1


LF




 


336
1

M
Y




 


343
1


AIV




 


345
1


DY




 


346
1


NA




 


348
2
F

K
RY



 


350
2


KHWY




 


351
2


GEM
DN



 


352
2


EFIMV




 


356
1


PMS




 


357
2

N
YE
M



 


358
1
M
A
L




 


359
1
A

T




 


360
1


KQ




 


361
2
M

AQSV
T



 


363
1

C
AS




 


364
2


DNT
EV



 


365
2

IV
L
CM



 


366
1


CA




 


367
2


NDEM




 


368
2
LY

ADQ
N



 


369
4

KW
FHQ
MNRTV
CDEGS


 


370
4


LADEFHNRSTV
GQ



 


371
4


QGHINPRTWY
CS
F


 


373
1

S
A
G



 


376
1


L

IM


 


377
2
FHV

Y
W



 


378
1


ND




 


379
4
CDNP
AHT
KERS

GQ


 


380
3

GR
SKN
ACDQTV
E


 


383
1


TQ
S



 


386
2


DKS
EN



 


389
4
F
L
GCMPRT
HI
ADEKNQSV


 


390
1

T
NS




 


392
3
C
A
WFM
ISTV
Y


 


393
3
LM

KHR
Q
CITV


 


402
2


VFIL




 


403
2
ACT

F




 


405
1


YF




 


407
1


A
G



 


408
2

GN
VQS
I
T


 


409
2

R
VCQS
HI
T


 


410
4

PY
QEGHIR
CDKLMT



 


411
2
CDE

N
G



 


413
1


KP




 


414
3

A
KCHIQ
EGLNP



 


415
2
MNP
S
E




 


418
1


EN




 


421
2
PW

Q

H


 


422
4
Y
CESV
KGHQR
ANT
D


 


423
4
ACDEFHMTV

YG
Q
NS


 


424
3

CN
HDGIST
EPQV



 


425
4
ACEFKLMN

DP






QRSTV






 


426
2
GY

TAMQ




 


428
2
AMN
R
SV
EQ



 


429
4
M
SV
RACDGHKN
ILTWY
EFQ


 


431
1


SG




 


432
2

QT
HAM
E



 


436
2
I

L
MY



 


437
3
EFLW
H
CN
KT
M


 


440
1
M

L
I



 


443
2
G

A
R
Q


 


444
2

K
SNQT
P
DE


 


445
1
QS

A




 


447
3
VY

IKR
AEMQS
T


 


448
3
L

AHST
EMNPQV



 


453
1
V

A




 


455
1
G

S

A


 


457
2

Q
SD
NT



 


458
1


C

T


 


460
2

C
MAEG
QRS



 


461
3
F

RN
DEGQST
A


 


462
1


TS
Q



 


463
2
F

KGN
ADE



 


464
4
K
T
GADEFHVY
LR
CMNQS


 


465
2


I
ACGST



 


466
3

A
SEGKNT
P
D


 


467
1


EN




 


468
2


EANPQ
D



 


469
2
Y

LAN




 


470
2


A
M
IL


 


471
2
I

TN
EHQ



 


472
2
R
Q
EAGN
DS



 


473
2


S
LV
I


 


475
1


MI
T



 


476
1
E

N




 


480
2
DV
MQ
E
N



 


481
1
N
V
T




 


484
2
CH

KA




 


487
1
L

K




 


488
1
C
A
E




 


489
4
ADEFGMNSTV
Q
KR




 


490
4
CRS
NV
LIY
ADEFHM



 


491
4
IL
D
GACMNQ
EKSTVY



 


492
2

A
GTV
C



 


493
3
EM
APQ
SCGKV




 


494
2


LGIQV
D



 


495
1
Y

F




 


496
2
IK
EQS
A
PT



 


497
2
L
GNR
KMT




 


498
2
A
ES
P




 


499
1
M

F




 


500
3

HQ
VIY
LM
AC


 


501
1


EN
D



 


502
3
FL
QS
TH
ACRV
M


 


503
2
F

ALM
I



 


504
1


IL




 


506
2


LIV

M


 


509
2
STV

QA




 


510
2


ST
CV



 


511
2

Y
HIM




 


512
1
Q

C




 


513
4
AEILMQRY

TS
V
CGKN


 


515
2
AGV

HQ
N



 


516
1

DR
N




 


517
1


GP




 


519
2
W

AC
ST



 


522
1


SAK




 


525
3

G
E
ACPQS
FR


 


528
1


RK




 


529
1


KA




 


531
4
CD

VGN
AMT
EHKQRS


 


534
2
GM

VAS




 


535
2
AGM

ICST




 


536
2
K

TM
AFG



 


537
2
IV

EHNQ
KT



 


538
1
K

P




 


539
1


IV




 


540
2

D
LEQRV
AP



 


541
1
L

P
M



 


542
2
IL
QY
FM
P



 


543
1

D
E




 


544
2


RGNPQS
C









Example 12
Less Combinable Improved Variants with Enhanced Specific Activity or Growth Activity
Table 32 lists variants that were either in suitability groups B or C, or not listed in Table 31. These “less combinable” variants did not fit the criteria for combinability as described above, yet displayed improved performance for either specific activity or growth upon retest.







TABLE 32







Positions in MEA P. alba with less combinable improved mutations that displayed PI


specific activity values >1.3

















Charge
% Surface



Residue
Position
Mutation
Location
Change
Accessibility
Function
















S
22
K
N-term
1
54
alternate surface interactions


S
22
R
N-term
1
54
alternate surface interactions


K
36
H
N-term
−1
33
alternate interactions with








nearby residues


K
36
W
N-term
−1
33
alternate interactions with








nearby residues


R
43
E
N-term
−2
52
alternate interactions with








nearby residues


E
58
F
N-term
1
20
alternate interactions with








nearby residues


G
87
S
surface
0
35
alternate surface interactions





loop


G
87
R
surface
1
35
alternate surface interactions





loop


F
89
D
Buried
−1
5
alternate interactions in pocket


F
89
E
Buried
−1
5
alternate interactions in pocket


A
118
E
Buried
−1
10
alternate interactions in pocket


A
118
P
Buried
0
10
alternate interactions in pocket


L
151
Y
surface
0
14
alternate interactions in pocket





loop


G
153
P
surface
0
40
affect shape of loop





loop


Q
234
R
Hinge
1
24
alternate surface interactions





Region


V
247
I
dimer
0
5
alternate interactions in pocket





interface


H
254
C
surface
0
89
alternate surface interactions





loop


S
282
H
Buried
0
8
alternate interactions in pocket


S
282
W
Buried
0
8
alternate interactions in pocket


S
288
A
Buried
0
0
alternate interactions in pocket


S
288
T
Buried
0
0
alternate interactions in pocket


S
288
Y
Buried
0
0
alternate interactions in pocket


A
391
G
Buried
0
0
alternate interactions in pocket


W
392
C
Buried
0
7
alternate interactions in pocket


C
437
L
Buried
0
7
alternate interactions in pocket


A
443
G
SubLoop
0
4
affect shape of loop


A
443
S
SubLoop
0
4
affect shape of loop


I
447
V
SubLoop
0
23
alternate interactions in pocket


T
481
Y
Buried
0
0
alternate interactions in pocket


E
488
L
Buried
1
13
alternate interactions in pocket


T
502
F
Buried
0
1
alternate interactions in pocket


F
542
N
surface
0
17
alternate interactions with





loop


nearby residues
















TABLE 33







Positions in MEA P. alba with less combinable improved mutations that displayed PI


values >1.3 for OD Max at 40 uM and 100 uM IPTG

















Charge
% Surface



Residue
Position
Mutation
Location
Change
Accessibility
Function
















V
30
K
N-term
1
60
replace surface hydrophobic w/








charged


K
134
C
N-term
−1
37
size reduction, improved





helix


interaction with neighboring His


K
134
P
N-term
−1
37
loop stabilization





helix


L
143
I
N-term
0
1
alternate interactions in





helix


hydrophobic pocket


I
156
G
N-term
0
13
allow more flexibility in loop





helix


E
159
D
N-term
0
32
alternate interactions with





helix


nearby residues


E
159
G
N-term
1
32
alternate interactions with





helix


nearby residues


E
159
Q
N-term
1
32
alternate interactions with





helix


nearby residues


S
172
V
N-term
0
47
affect shape of loop





helix


K
414
F
surface
−1
70
alternate surface interactions





loop


Q
421
R
surface
1
23
alternate surface interactions





loop


Q
421
D
surface
−1
23
alternate surface interactions





loop









Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention, which are obvious to those skilled in the relevant fields, are intended to be within the scope of the following claims.


















APPENDIX 1







ATOM
1
N
SER
A
6
57.852
−35.562
−29.199
1.00
48.03
N


ATOM
2
CA
SER
A
6
58.221
−34.126
−29.362
1.00
48.39
C


ATOM
4
CB
SER
A
6
56.988
−33.233
−29.166
1.00
48.63
C


ATOM
7
OG
SER
A
6
57.237
−31.904
−29.599
1.00
47.21
O


ATOM
9
C
SER
A
6
59.320
−33.739
−28.363
1.00
48.42
C


ATOM
10
O
SER
A
6
59.140
−33.874
−27.147
1.00
48.63
O


ATOM
14
N
ALA
A
7
60.451
−33.258
−28.882
1.00
47.57
N


ATOM
15
CA
ALA
A
7
61.585
−32.851
−28.052
1.00
46.90
C


ATOM
17
CB
ALA
A
7
62.883
−33.002
−28.822
1.00
46.86
C


ATOM
21
C
ALA
A
7
61.428
−31.414
−27.567
1.00
46.82
C


ATOM
22
O
ALA
A
7
60.887
−30.566
−28.275
1.00
46.12
O


ATOM
24
N
ASN
A
8
61.910
−31.151
−26.354
1.00
47.66
N


ATOM
25
CA
ASN
A
8
61.854
−29.817
−25.756
1.00
48.08
C


ATOM
27
CB
ASN
A
8
61.091
−29.857
−24.426
1.00
48.06
C


ATOM
30
CG
ASN
A
8
60.878
−28.470
−23.828
1.00
49.99
C


ATOM
31
OD1
ASN
A
8
60.837
−27.463
−24.546
1.00
51.29
O


ATOM
32
ND2
ASN
A
8
60.734
−28.413
−22.508
1.00
49.83
N


ATOM
35
C
ASN
A
8
63.253
−29.233
−25.544
1.00
48.15
C


ATOM
36
O
ASN
A
8
63.937
−29.570
−24.574
1.00
47.83
O


ATOM
38
N
TYR
A
9
63.659
−28.351
−26.456
1.00
48.38
N


ATOM
39
CA
TYR
A
9
64.948
−27.667
−26.370
1.00
48.44
C


ATOM
41
CB
TYR
A
9
65.635
−27.673
−27.736
1.00
47.95
C


ATOM
44
CG
TYR
A
9
65.797
−29.046
−28.349
1.00
44.89
C


ATOM
45
CD1
TYR
A
9
66.464
−30.064
−27.668
1.00
41.90
C


ATOM
47
CE1
TYR
A
9
66.619
−31.321
−28.237
1.00
40.74
C


ATOM
49
CZ
TYR
A
9
66.110
−31.565
−29.506
1.00
38.55
C


ATOM
50
OH
TYR
A
9
66.253
−32.801
−30.091
1.00
37.89
O


ATOM
52
CE2
TYR
A
9
65.452
−30.574
−30.198
1.00
37.42
C


ATOM
54
CD2
TYR
A
9
65.300
−29.323
−29.623
1.00
40.75
C


ATOM
56
C
TYR
A
9
64.809
−26.220
−25.884
1.00
49.82
C


ATOM
57
O
TYR
A
9
65.778
−25.458
−25.923
1.00
49.95
O


ATOM
59
N
GLU
A
10
63.615
−25.836
−25.434
1.00
51.30
N


ATOM
60
CA
GLU
A
10
63.393
−24.476
−24.935
1.00
52.39
C


ATOM
62
CB
GLU
A
10
61.901
−24.104
−24.946
1.00
53.08
C


ATOM
65
CG
GLU
A
10
61.341
−23.787
−26.340
1.00
55.31
C


ATOM
68
CD
GLU
A
10
61.948
−22.531
−26.962
1.00
58.35
C


ATOM
69
OE1
GLU
A
10
61.645
−21.419
−26.475
1.00
58.63
O


ATOM
70
OE2
GLU
A
10
62.719
−22.656
−27.943
1.00
58.45
O


ATOM
71
C
GLU
A
10
63.970
−24.336
−23.527
1.00
52.09
C


ATOM
72
O
GLU
A
10
63.803
−25.235
−22.701
1.00
51.48
O


ATOM
74
N
PRO
A
11
64.654
−23.206
−23.252
1.00
52.23
N


ATOM
75
CA
PRO
A
11
65.326
−23.016
−21.968
1.00
51.84
C


ATOM
77
CB
PRO
A
11
66.232
−21.808
−22.226
1.00
51.99
C


ATOM
80
CG
PRO
A
11
65.483
−21.004
−23.234
1.00
52.61
C


ATOM
83
CD
PRO
A
11
64.767
−22.007
−24.109
1.00
52.51
C


ATOM
86
C
PRO
A
11
64.352
−22.710
−20.838
1.00
50.93
C


ATOM
87
O
PRO
A
11
63.336
−22.054
−21.060
1.00
51.02
O


ATOM
88
N
ASN
A
12
64.671
−23.192
−19.641
1.00
50.14
N


ATOM
89
CA
ASN
A
12
63.903
−22.874
−18.442
1.00
49.76
C


ATOM
91
CB
ASN
A
12
64.149
−23.932
−17.361
1.00
50.42
C


ATOM
94
CG
ASN
A
12
63.646
−25.311
−17.765
1.00
51.17
C


ATOM
95
OD1
ASN
A
12
64.408
−26.280
−17.814
1.00
51.99
O


ATOM
96
ND2
ASN
A
12
62.357
−25.400
−18.065
1.00
53.64
N


ATOM
99
C
ASN
A
12
64.266
−21.489
−17.904
1.00
48.64
C


ATOM
100
O
ASN
A
12
65.371
−20.991
−18.139
1.00
47.42
O


ATOM
102
N
SER
A
13
63.331
−20.881
−17.175
1.00
48.28
N


ATOM
103
CA
SER
A
13
63.564
−19.584
−16.514
1.00
47.88
C


ATOM
105
CB
SER
A
13
62.232
−18.959
−16.071
1.00
47.65
C


ATOM
108
OG
SER
A
13
61.426
−19.896
−15.372
1.00
45.33
O


ATOM
110
C
SER
A
13
64.522
−19.677
−15.310
1.00
47.96
C


ATOM
111
O
SER
A
13
64.897
−18.650
−14.738
1.00
48.86
O


ATOM
113
N
TRP
A
14
64.901
−20.901
−14.930
1.00
47.44
N


ATOM
114
CA
TRP
A
14
65.867
−21.142
−13.848
1.00
46.79
C


ATOM
116
CB
TRP
A
14
65.278
−22.098
−12.793
1.00
47.11
C


ATOM
119
CG
TRP
A
14
64.534
−23.290
−13.337
1.00
49.29
C


ATOM
120
CD1
TRP
A
14
63.180
−23.428
−13.440
1.00
52.50
C


ATOM
122
NE1
TRP
A
14
62.866
−24.650
−13.981
1.00
54.55
N


ATOM
124
CE2
TRP
A
14
64.026
−25.333
−14.235
1.00
55.28
C


ATOM
125
CD2
TRP
A
14
65.100
−24.504
−13.842
1.00
51.73
C


ATOM
126
CE3
TRP
A
14
66.410
−24.972
−14.004
1.00
55.03
C


ATOM
128
CZ3
TRP
A
14
66.607
−26.239
−14.548
1.00
58.13
C


ATOM
130
CH2
TRP
A
14
65.516
−27.041
−14.928
1.00
60.71
C


ATOM
132
CZ2
TRP
A
14
64.222
−26.607
−14.778
1.00
58.33
C


ATOM
134
C
TRP
A
14
67.228
−21.664
−14.343
1.00
46.01
C


ATOM
135
O
TRP
A
14
68.079
−22.022
−13.538
1.00
45.81
O


ATOM
137
N
ASP
A
15
67.445
−21.692
−15.656
1.00
45.26
N


ATOM
138
CA
ASP
A
15
68.724
−22.154
−16.200
1.00
44.40
C


ATOM
140
CB
ASP
A
15
68.628
−22.388
−17.715
1.00
44.60
C


ATOM
143
CG
ASP
A
15
67.990
−23.730
−18.068
1.00
46.58
C


ATOM
144
OD1
ASP
A
15
68.236
−24.739
−17.357
1.00
48.71
O


ATOM
145
OD2
ASP
A
15
67.253
−23.779
−19.079
1.00
48.05
O


ATOM
146
C
ASP
A
15
69.843
−21.163
−15.886
1.00
43.10
C


ATOM
147
O
ASP
A
15
69.627
−19.956
−15.900
1.00
42.96
O


ATOM
149
N
TYR
A
16
71.037
−21.682
−15.608
1.00
42.15
N


ATOM
150
CA
TYR
A
16
72.183
−20.841
−15.252
1.00
41.48
C


ATOM
152
CB
TYR
A
16
73.367
−21.689
−14.758
1.00
40.41
C


ATOM
155
CG
TYR
A
16
73.116
−22.434
−13.462
1.00
34.99
C


ATOM
156
CD1
TYR
A
16
72.755
−21.754
−12.302
1.00
31.52
C


ATOM
158
CE1
TYR
A
16
72.523
−22.434
−11.109
1.00
26.87
C


ATOM
160
CZ
TYR
A
16
72.661
−23.806
−11.068
1.00
26.53
C


ATOM
161
OH
TYR
A
16
72.428
−24.472
−9.884
1.00
23.41
O


ATOM
163
CE2
TYR
A
16
73.028
−24.506
−12.207
1.00
24.79
C


ATOM
165
CD2
TYR
A
16
73.255
−23.820
−13.391
1.00
28.38
C


ATOM
167
C
TYR
A
16
72.645
−19.941
−16.405
1.00
43.23
C


ATOM
168
O
TYR
A
16
73.366
−18.975
−16.170
1.00
42.92
O


ATOM
170
N
ASP
A
17
72.260
−20.272
−17.639
1.00
45.46
N


ATOM
171
CA
ASP
A
17
72.480
−19.381
−18.789
1.00
47.17
C


ATOM
173
CB
ASP
A
17
72.177
−20.100
−20.123
1.00
47.23
C


ATOM
176
CG
ASP
A
17
73.341
−20.971
−20.625
1.00
48.12
C


ATOM
177
OD1
ASP
A
17
74.518
−20.677
−20.315
1.00
47.64
O


ATOM
178
OD2
ASP
A
17
73.074
−21.946
−21.364
1.00
47.90
O


ATOM
179
C
ASP
A
17
71.607
−18.119
−18.661
1.00
48.86
C


ATOM
180
O
ASP
A
17
72.085
−16.998
−18.874
1.00
48.22
O


ATOM
182
N
TYR
A
18
70.337
−18.315
−18.301
1.00
50.67
N


ATOM
183
CA
TYR
A
18
69.374
−17.218
−18.165
1.00
52.74
C


ATOM
185
CB
TYR
A
18
67.939
−17.763
−18.164
1.00
53.75
C


ATOM
188
CG
TYR
A
18
66.859
−16.697
−18.156
1.00
60.91
C


ATOM
189
CD1
TYR
A
18
66.458
−16.071
−19.339
1.00
65.86
C


ATOM
191
CE1
TYR
A
18
65.463
−15.095
−19.335
1.00
68.28
C


ATOM
193
CZ
TYR
A
18
64.855
−14.737
−18.137
1.00
70.56
C


ATOM
194
OH
TYR
A
18
63.869
−13.774
−18.121
1.00
72.76
O


ATOM
196
CE2
TYR
A
18
65.233
−15.346
−16.950
1.00
68.19
C


ATOM
198
CD2
TYR
A
18
66.227
−16.322
−16.965
1.00
65.97
C


ATOM
200
C
TYR
A
18
69.656
−16.396
−16.906
1.00
52.94
C


ATOM
201
O
TYR
A
18
70.007
−15.222
−17.004
1.00
53.20
O


ATOM
203
N
LEU
A
19
69.507
−17.011
−15.730
1.00
53.94
N


ATOM
204
CA
LEU
A
19
69.968
−16.411
−14.472
1.00
54.31
C


ATOM
206
CB
LEU
A
19
69.536
−17.246
−13.261
1.00
53.90
C


ATOM
209
CG
LEU
A
19
68.046
−17.390
−12.950
1.00
53.47
C


ATOM
211
CD1
LEU
A
19
67.848
−18.469
−11.894
1.00
52.00
C


ATOM
215
CD2
LEU
A
19
67.445
−16.067
−12.493
1.00
52.71
C


ATOM
219
C
LEU
A
19
71.483
−16.381
−14.523
1.00
55.57
C


ATOM
220
O
LEU
A
19
72.078
−16.875
−15.480
1.00
56.83
O


ATOM
222
N
LEU
A
20
72.121
−15.805
−13.510
1.00
56.21
N


ATOM
223
CA
LEU
A
20
73.577
−15.890
−13.397
1.00
56.88
C


ATOM
225
CB
LEU
A
20
73.968
−17.352
−13.124
1.00
56.40
C


ATOM
228
CG
LEU
A
20
74.997
−17.636
−12.034
1.00
55.69
C


ATOM
230
CD1
LEU
A
20
74.571
−17.015
−10.709
1.00
53.68
C


ATOM
234
CD2
LEU
A
20
75.192
−19.138
−11.885
1.00
52.71
C


ATOM
238
C
LEU
A
20
74.295
−15.361
−14.659
1.00
58.48
C


ATOM
239
O
LEU
A
20
75.410
−15.788
−14.977
1.00
58.46
O


ATOM
241
N
SER
A
21
73.650
−14.432
−15.368
1.00
60.28
N


ATOM
242
CA
SER
A
21
74.213
−13.838
−16.577
1.00
61.62
C


ATOM
244
CB
SER
A
21
73.171
−13.789
−17.701
1.00
61.64
C


ATOM
247
OG
SER
A
21
73.794
−13.866
−18.974
1.00
59.12
O


ATOM
249
C
SER
A
21
74.719
−12.437
−16.246
1.00
63.66
C


ATOM
250
O
SER
A
21
74.176
−11.759
−15.365
1.00
63.30
O


ATOM
252
N
SER
A
22
75.756
−12.014
−16.966
1.00
66.64
N


ATOM
253
CA
SER
A
22
76.506
−10.806
−16.625
1.00
68.86
C


ATOM
255
CB
SER
A
22
77.810
−10.746
−17.433
1.00
69.20
C


ATOM
258
OG
SER
A
22
77.555
−10.584
−18.820
1.00
69.64
O


ATOM
260
C
SER
A
22
75.720
−9.518
−16.850
1.00
70.68
C


ATOM
261
O
SER
A
22
74.900
−9.426
−17.769
1.00
71.20
O


ATOM
263
N
ASP
A
23
75.978
−8.534
−15.990
1.00
72.36
N


ATOM
264
CA
ASP
A
23
75.526
−7.162
−16.208
1.00
73.53
C


ATOM
266
CB
ASP
A
23
75.173
−6.482
−14.876
1.00
73.91
C


ATOM
269
CG
ASP
A
23
74.200
−7.312
−14.024
1.00
76.32
C


ATOM
270
OD1
ASP
A
23
73.930
−8.489
−14.364
1.00
78.08
O


ATOM
271
OD2
ASP
A
23
73.708
−6.787
−13.000
1.00
78.94
O


ATOM
272
C
ASP
A
23
76.683
−6.472
−16.932
1.00
73.70
C


ATOM
273
O
ASP
A
23
76.527
−5.994
−18.056
1.00
73.41
O


ATOM
275
N
THR
A
24
77.839
−6.429
−16.269
1.00
74.34
N


ATOM
276
CA
THR
A
24
79.148
−6.312
−16.935
1.00
74.64
C


ATOM
278
CB
THR
A
24
79.549
−4.836
−17.203
1.00
74.73
C


ATOM
280
OG1
THR
A
24
78.431
−4.113
−17.732
1.00
75.12
O


ATOM
282
CG2
THR
A
24
80.707
−4.755
−18.199
1.00
74.19
C


ATOM
286
C
THR
A
24
80.233
−7.012
−16.086
1.00
74.73
C


ATOM
287
O
THR
A
24
81.413
−6.648
−16.143
1.00
74.53
O


ATOM
289
N
ASP
A
25
79.821
−8.029
−15.321
1.00
74.69
N


ATOM
290
CA
ASP
A
25
80.692
−8.720
−14.362
1.00
74.42
C


ATOM
292
CB
ASP
A
25
79.931
−9.032
−13.064
1.00
74.45
C


ATOM
295
CG
ASP
A
25
79.439
−7.782
−12.345
1.00
75.87
C


ATOM
296
OD1
ASP
A
25
80.097
−6.723
−12.442
1.00
78.52
O


ATOM
297
OD2
ASP
A
25
78.390
−7.868
−11.668
1.00
75.22
O


ATOM
298
C
ASP
A
25
81.208
−10.032
−14.949
1.00
73.89
C


ATOM
299
O
ASP
A
25
80.416
−10.901
−15.329
1.00
74.17
O


ATOM
301
N
GLU
A
26
82.533
−10.174
−15.005
1.00
72.59
N


ATOM
302
CA
GLU
A
26
83.176
−11.412
−15.464
1.00
71.44
C


ATOM
304
CB
GLU
A
26
84.542
−11.099
−16.083
1.00
71.52
C


ATOM
307
CG
GLU
A
26
84.462
−10.218
−17.329
1.00
72.73
C


ATOM
310
CD
GLU
A
26
85.791
−9.577
−17.706
1.00
73.49
C


ATOM
311
OE1
GLU
A
26
86.857
−10.178
−17.448
1.00
75.86
O


ATOM
312
OE2
GLU
A
26
85.764
−8.465
−18.273
1.00
72.72
O


ATOM
313
C
GLU
A
26
83.328
−12.449
−14.338
1.00
70.01
C


ATOM
314
O
GLU
A
26
83.745
−13.583
−14.589
1.00
70.09
O


ATOM
316
N
SER
A
27
82.996
−12.054
−13.106
1.00
68.20
N


ATOM
317
CA
SER
A
27
82.992
−12.965
−11.953
1.00
66.37
C


ATOM
319
CB
SER
A
27
83.203
−12.185
−10.647
1.00
66.19
C


ATOM
322
OG
SER
A
27
82.281
−11.114
−10.526
1.00
64.64
O


ATOM
324
C
SER
A
27
81.696
−13.783
−11.874
1.00
64.84
C


ATOM
325
O
SER
A
27
81.675
−14.864
−11.278
1.00
64.63
O


ATOM
327
N
ILE
A
28
80.623
−13.266
−12.471
1.00
62.61
N


ATOM
328
CA
ILE
A
28
79.354
−13.989
−12.539
1.00
61.02
C


ATOM
330
CB
ILE
A
28
78.169
−13.038
−12.860
1.00
61.01
C


ATOM
332
CG1
ILE
A
28
78.014
−11.979
−11.759
1.00
61.43
C


ATOM
335
CD1
ILE
A
28
76.755
−11.111
−11.888
1.00
61.10
C


ATOM
339
CG2
ILE
A
28
76.870
−13.822
−13.007
1.00
59.79
C


ATOM
343
C
ILE
A
28
79.409
−15.124
−13.569
1.00
60.14
C


ATOM
344
O
ILE
A
28
78.617
−16.063
−13.490
1.00
60.44
O


ATOM
346
N
GLU
A
29
80.349
−15.051
−14.514
1.00
58.86
N


ATOM
347
CA
GLU
A
29
80.458
−16.054
−15.583
1.00
58.37
C


ATOM
349
CB
GLU
A
29
80.872
−15.396
−16.903
1.00
59.02
C


ATOM
352
CG
GLU
A
29
79.753
−14.583
−17.554
1.00
61.53
C


ATOM
355
CD
GLU
A
29
80.073
−14.176
−18.984
1.00
65.33
C


ATOM
356
OE1
GLU
A
29
80.488
−15.052
−19.775
1.00
67.45
O


ATOM
357
OE2
GLU
A
29
79.904
−12.982
−19.320
1.00
67.36
O


ATOM
358
C
GLU
A
29
81.396
−17.229
−15.256
1.00
57.04
C


ATOM
359
O
GLU
A
29
81.179
−18.343
−15.739
1.00
57.21
O


ATOM
361
N
VAL
A
30
82.438
−16.983
−14.463
1.00
55.15
N


ATOM
362
CA
VAL
A
30
83.268
−18.068
−13.914
1.00
53.30
C


ATOM
364
CB
VAL
A
30
84.483
−17.510
−13.117
1.00
53.38
C


ATOM
366
CG1
VAL
A
30
85.039
−18.555
−12.151
1.00
52.60
C


ATOM
370
CG2
VAL
A
30
85.573
−17.023
−14.073
1.00
53.38
C


ATOM
374
C
VAL
A
30
82.404
−18.950
−13.004
1.00
51.50
C


ATOM
375
O
VAL
A
30
82.421
−20.180
−13.099
1.00
51.26
O


ATOM
377
N
TYR
A
31
81.660
−18.280
−12.129
1.00
49.11
N


ATOM
378
CA
TYR
A
31
80.650
−18.870
−11.254
1.00
47.25
C


ATOM
380
CB
TYR
A
31
79.947
−17.705
−10.543
1.00
47.92
C


ATOM
383
CG
TYR
A
31
79.090
−18.009
−9.336
1.00
51.28
C


ATOM
384
CD1
TYR
A
31
79.633
−18.024
−8.051
1.00
55.50
C


ATOM
386
CE1
TYR
A
31
78.826
−18.268
−6.923
1.00
59.62
C


ATOM
388
CZ
TYR
A
31
77.454
−18.472
−7.083
1.00
62.10
C


ATOM
389
OH
TYR
A
31
76.634
−18.714
−5.992
1.00
59.44
O


ATOM
391
CE2
TYR
A
31
76.898
−18.436
−8.354
1.00
61.47
C


ATOM
393
CD2
TYR
A
31
77.717
−18.193
−9.468
1.00
57.47
C


ATOM
395
C
TYR
A
31
79.647
−19.714
−12.064
1.00
45.23
C


ATOM
396
O
TYR
A
31
79.352
−20.858
−11.713
1.00
44.76
O


ATOM
398
N
LYS
A
32
79.154
−19.138
−13.163
1.00
42.92
N


ATOM
399
CA
LYS
A
32
78.141
−19.767
−14.032
1.00
40.55
C


ATOM
401
CB
LYS
A
32
77.680
−18.764
−15.106
1.00
40.76
C


ATOM
404
CG
LYS
A
32
77.038
−19.362
−16.366
1.00
40.67
C


ATOM
407
CD
LYS
A
32
76.894
−18.308
−17.472
1.00
42.03
C


ATOM
410
CE
LYS
A
32
77.173
−18.889
−18.857
1.00
42.97
C


ATOM
413
NZ
LYS
A
32
77.209
−17.838
−19.913
1.00
42.04
N


ATOM
417
C
LYS
A
32
78.596
−21.068
−14.700
1.00
38.15
C


ATOM
418
O
LYS
A
32
77.855
−22.048
−14.695
1.00
38.36
O


ATOM
420
N
ASP
A
33
79.790
−21.070
−15.292
1.00
35.64
N


ATOM
421
CA
ASP
A
33
80.311
−22.259
−15.982
1.00
33.78
C


ATOM
423
CB
ASP
A
33
81.601
−21.929
−16.738
1.00
34.72
C


ATOM
426
CG
ASP
A
33
81.354
−21.081
−17.969
1.00
36.54
C


ATOM
427
OD1
ASP
A
33
80.594
−21.527
−18.859
1.00
40.72
O


ATOM
428
OD2
ASP
A
33
81.928
−19.975
−18.048
1.00
37.20
O


ATOM
429
C
ASP
A
33
80.573
−23.393
−15.009
1.00
31.24
C


ATOM
430
O
ASP
A
33
80.470
−24.561
−15.367
1.00
30.51
O


ATOM
432
N
LYS
A
34
80.914
−23.027
−13.777
1.00
29.73
N


ATOM
433
CA
LYS
A
34
81.107
−23.977
−12.684
1.00
28.39
C


ATOM
435
CB
LYS
A
34
81.706
−23.259
−11.469
1.00
29.61
C


ATOM
438
CG
LYS
A
34
82.593
−24.116
−10.557
1.00
33.47
C


ATOM
441
CD
LYS
A
34
83.235
−23.222
−9.474
1.00
38.41
C


ATOM
444
CE
LYS
A
34
84.737
−23.462
−9.306
1.00
40.10
C


ATOM
447
NZ
LYS
A
34
85.408
−22.281
−8.672
1.00
38.56
N


ATOM
451
C
LYS
A
34
79.782
−24.605
−12.286
1.00
25.39
C


ATOM
452
O
LYS
A
34
79.742
−25.751
−11.879
1.00
25.45
O


ATOM
454
N
ALA
A
35
78.701
−23.844
−12.387
1.00
23.04
N


ATOM
455
CA
ALA
A
35
77.375
−24.362
−12.075
1.00
21.87
C


ATOM
457
CB
ALA
A
35
76.354
−23.237
−12.076
1.00
21.62
C


ATOM
461
C
ALA
A
35
76.985
−25.436
−13.082
1.00
20.42
C


ATOM
462
O
ALA
A
35
76.625
−26.554
−12.713
1.00
19.70
O


ATOM
464
N
LYS
A
36
77.081
−25.082
−14.357
1.00
18.77
N


ATOM
465
CA
LYS
A
36
76.691
−25.966
−15.444
1.00
18.24
C


ATOM
467
CB
LYS
A
36
76.929
−25.269
−16.789
1.00
18.00
C


ATOM
470
CG
LYS
A
36
75.987
−24.105
−17.043
1.00
18.92
C


ATOM
473
CD
LYS
A
36
76.557
−23.085
−18.024
1.00
21.70
C


ATOM
476
CE
LYS
A
36
76.472
−23.540
−19.465
1.00
19.59
C


ATOM
479
NZ
LYS
A
36
77.258
−22.646
−20.331
1.00
19.67
N


ATOM
483
C
LYS
A
36
77.446
−27.294
−15.387
1.00
17.53
C


ATOM
484
O
LYS
A
36
76.857
−28.356
−15.554
1.00
17.14
O


ATOM
486
N
LYS
A
37
78.746
−27.223
−15.131
1.00
17.40
N


ATOM
487
CA
LYS
A
37
79.589
−28.417
−15.044
1.00
17.76
C


ATOM
489
CB
LYS
A
37
81.051
−27.991
−14.895
1.00
18.24
C


ATOM
492
CG
LYS
A
37
82.076
−29.104
−15.034
1.00
22.01
C


ATOM
495
CD
LYS
A
37
83.467
−28.513
−15.299
1.00
28.55
C


ATOM
498
CE
LYS
A
37
84.524
−29.586
−15.533
1.00
29.97
C


ATOM
501
NZ
LYS
A
37
84.955
−30.203
−14.254
1.00
33.41
N


ATOM
505
C
LYS
A
37
79.165
−29.324
−13.875
1.00
16.90
C


ATOM
506
O
LYS
A
37
79.098
−30.549
−14.016
1.00
17.17
O


ATOM
508
N
LEU
A
38
78.872
−28.713
−12.729
1.00
15.23
N


ATOM
509
CA
LEU
A
38
78.439
−29.450
−11.545
1.00
13.91
C


ATOM
511
CB
LEU
A
38
78.450
−28.535
−10.316
1.00
13.03
C


ATOM
514
CG
LEU
A
38
79.814
−27.984
−9.882
1.00
10.93
C


ATOM
516
CD1
LEU
A
38
79.634
−26.844
−8.889
1.00
9.71
C


ATOM
520
CD2
LEU
A
38
80.688
−29.059
−9.291
1.00
6.81
C


ATOM
524
C
LEU
A
38
77.044
−30.030
−11.753
1.00
14.10
C


ATOM
525
O
LEU
A
38
76.768
−31.184
−11.386
1.00
14.32
O


ATOM
527
N
GLU
A
39
76.175
−29.215
−12.349
1.00
14.06
N


ATOM
528
CA
GLU
A
39
74.796
−29.597
−12.644
1.00
13.26
C


ATOM
530
CB
GLU
A
39
74.045
−28.406
−13.243
1.00
13.22
C


ATOM
533
CG
GLU
A
39
72.555
−28.631
−13.379
1.00
16.71
C


ATOM
536
CD
GLU
A
39
71.829
−27.489
−14.055
1.00
16.18
C


ATOM
537
OE1
GLU
A
39
72.470
−26.668
−14.731
1.00
16.33
O


ATOM
538
OE2
GLU
A
39
70.596
−27.425
−13.921
1.00
20.26
O


ATOM
539
C
GLU
A
39
74.764
−30.772
−13.610
1.00
12.52
C


ATOM
540
O
GLU
A
39
74.024
−31.729
−13.413
1.00
12.91
O


ATOM
542
N
ALA
A
40
75.581
−30.698
−14.652
1.00
11.95
N


ATOM
543
CA
ALA
A
40
75.680
−31.779
−15.616
1.00
11.90
C


ATOM
545
CB
ALA
A
40
76.712
−31.435
−16.686
1.00
11.71
C


ATOM
549
C
ALA
A
40
76.026
−33.114
−14.944
1.00
12.35
C


ATOM
550
O
ALA
A
40
75.418
−34.142
−15.241
1.00
12.86
O


ATOM
552
N
GLU
A
41
77.001
−33.098
−14.040
1.00
12.31
N


ATOM
553
CA
GLU
A
41
77.411
−34.316
−13.341
1.00
11.84
C


ATOM
555
CB
GLU
A
41
78.657
−34.063
−12.503
1.00
12.96
C


ATOM
558
CG
GLU
A
41
79.319
−35.339
−11.995
1.00
16.43
C


ATOM
561
CD
GLU
A
41
80.689
−35.094
−11.380
1.00
20.65
C


ATOM
562
OE1
GLU
A
41
81.384
−34.141
−11.813
1.00
21.41
O


ATOM
563
OE2
GLU
A
41
81.063
−35.863
−10.464
1.00
22.80
O


ATOM
564
C
GLU
A
41
76.318
−34.881
−12.445
1.00
11.21
C


ATOM
565
O
GLU
A
41
76.197
−36.096
−12.317
1.00
10.59
O


ATOM
567
N
VAL
A
42
75.533
−34.014
−11.812
1.00
10.59
N


ATOM
568
CA
VAL
A
42
74.455
−34.504
−10.953
1.00
10.83
C


ATOM
570
CB
VAL
A
42
73.874
−33.410
−10.059
1.00
9.83
C


ATOM
572
CG1
VAL
A
42
72.666
−33.930
−9.327
1.00
8.79
C


ATOM
576
CG2
VAL
A
42
74.927
−32.942
−9.073
1.00
6.37
C


ATOM
580
C
VAL
A
42
73.364
−35.139
−11.801
1.00
12.18
C


ATOM
581
O
VAL
A
42
72.809
−36.175
−11.434
1.00
13.46
O


ATOM
583
N
ARG
A
43
73.082
−34.524
−12.944
1.00
13.15
N


ATOM
584
CA
ARG
A
43
72.164
−35.088
−13.933
1.00
13.17
C


ATOM
586
CB
ARG
A
43
72.038
−34.128
−15.121
1.00
13.87
C


ATOM
589
CG
ARG
A
43
71.145
−34.608
−16.242
1.00
15.13
C


ATOM
592
CD
ARG
A
43
71.899
−35.461
−17.242
1.00
15.36
C


ATOM
595
NE
ARG
A
43
70.996
−36.139
−18.157
1.00
13.99
N


ATOM
597
CZ
ARG
A
43
71.379
−37.012
−19.079
1.00
12.96
C


ATOM
598
NH1
ARG
A
43
70.469
−37.573
−19.859
1.00
14.01
N


ATOM
601
NH2
ARG
A
43
72.665
−37.326
−19.234
1.00
16.32
N


ATOM
604
C
ARG
A
43
72.629
−36.471
−14.402
1.00
12.96
C


ATOM
605
O
ARG
A
43
71.830
−37.399
−14.510
1.00
12.10
O


ATOM
607
N
ARG
A
44
73.919
−36.604
−14.689
1.00
13.18
N


ATOM
608
CA
ARG
A
44
74.473
−37.899
−15.083
1.00
13.52
C


ATOM
610
CB
ARG
A
44
75.973
−37.796
−15.348
1.00
13.04
C


ATOM
613
CG
ARG
A
44
76.587
−39.071
−15.934
1.00
13.55
C


ATOM
616
CD
ARG
A
44
78.096
−38.945
−16.116
1.00
10.92
C


ATOM
619
NE
ARG
A
44
78.768
−38.750
−14.837
1.00
10.95
N


ATOM
621
CZ
ARG
A
44
80.014
−38.310
−14.688
1.00
13.62
C


ATOM
622
NH1
ARG
A
44
80.511
−38.162
−13.470
1.00
18.93
N


ATOM
625
NH2
ARG
A
44
80.772
−38.013
−15.735
1.00
13.99
N


ATOM
628
C
ARG
A
44
74.206
−38.967
−14.017
1.00
13.83
C


ATOM
629
O
ARG
A
44
73.723
−40.046
−14.329
1.00
14.61
O


ATOM
631
N
GLU
A
45
74.497
−38.652
−12.761
1.00
13.69
N


ATOM
632
CA
GLU
A
45
74.333
−39.615
−11.673
1.00
13.28
C


ATOM
634
CB
GLU
A
45
75.006
−39.097
−10.400
1.00
13.92
C


ATOM
637
CG
GLU
A
45
76.501
−38.906
−10.534
1.00
13.86
C


ATOM
640
CD
GLU
A
45
77.179
−40.128
−11.080
1.00
15.34
C


ATOM
641
OE1
GLU
A
45
76.958
−41.210
−10.505
1.00
21.32
O


ATOM
642
OE2
GLU
A
45
77.917
−40.010
−12.085
1.00
15.78
O


ATOM
643
C
GLU
A
45
72.876
−39.953
−11.378
1.00
13.56
C


ATOM
644
O
GLU
A
45
72.577
−41.089
−11.022
1.00
12.52
O


ATOM
646
N
ILE
A
46
71.976
−38.974
−11.510
1.00
13.94
N


ATOM
647
CA
ILE
A
46
70.543
−39.242
−11.371
1.00
13.70
C


ATOM
649
CB
ILE
A
46
69.698
−37.947
−11.340
1.00
13.76
C


ATOM
651
CG1
ILE
A
46
69.950
−37.174
−10.052
1.00
13.82
C


ATOM
654
CD1
ILE
A
46
69.283
−35.834
−10.025
1.00
12.62
C


ATOM
658
CG2
ILE
A
46
68.219
−38.269
−11.388
1.00
9.73
C


ATOM
662
C
ILE
A
46
70.015
−40.170
−12.475
1.00
14.01
C


ATOM
663
O
ILE
A
46
69.145
−40.996
−12.213
1.00
16.46
O


ATOM
665
N
ASN
A
47
70.541
−40.040
−13.692
1.00
13.63
N


ATOM
666
CA
ASN
A
47
70.114
−40.866
−14.826
1.00
12.95
C


ATOM
668
CB
ASN
A
47
70.261
−40.077
−16.126
1.00
13.09
C


ATOM
671
CG
ASN
A
47
69.156
−39.073
−16.327
1.00
12.33
C


ATOM
672
OD1
ASN
A
47
68.181
−39.363
−17.007
1.00
17.25
O


ATOM
673
ND2
ASN
A
47
69.301
−37.886
−15.747
1.00
6.46
N


ATOM
676
C
ASN
A
47
70.848
−42.209
−14.981
1.00
13.75
C


ATOM
677
O
ASN
A
47
70.493
−43.004
−15.859
1.00
14.81
O


ATOM
679
N
ASN
A
48
71.857
−42.467
−14.149
1.00
13.90
N


ATOM
680
CA
ASN
A
48
72.651
−43.713
−14.233
1.00
14.21
C


ATOM
682
CB
ASN
A
48
73.577
−43.823
−13.012
1.00
13.88
C


ATOM
685
CG
ASN
A
48
74.474
−45.057
−13.049
1.00
13.81
C


ATOM
686
OD1
ASN
A
48
74.410
−45.866
−13.971
1.00
14.91
O


ATOM
687
ND2
ASN
A
48
75.314
−45.201
−12.033
1.00
7.59
N


ATOM
690
C
ASN
A
48
71.775
−44.968
−14.343
1.00
15.13
C


ATOM
691
O
ASN
A
48
71.114
−45.346
−13.383
1.00
16.48
O


ATOM
693
N
GLU
A
49
71.790
−45.620
−15.505
1.00
15.77
N


ATOM
694
CA
GLU
A
49
70.895
−46.763
−15.762
1.00
16.49
C


ATOM
696
CB
GLU
A
49
70.848
−47.114
−17.256
1.00
16.02
C


ATOM
699
CG
GLU
A
49
70.388
−45.984
−18.170
1.00
17.82
C


ATOM
702
CD
GLU
A
49
71.524
−45.093
−18.666
1.00
20.53
C


ATOM
703
OE1
GLU
A
49
72.654
−45.198
−18.125
1.00
17.54
O


ATOM
704
OE2
GLU
A
49
71.276
−44.290
−19.599
1.00
18.89
O


ATOM
705
C
GLU
A
49
71.251
−48.032
−14.975
1.00
17.20
C


ATOM
706
O
GLU
A
49
70.432
−48.950
−14.899
1.00
17.66
O


ATOM
708
N
LYS
A
50
72.461
−48.092
−14.417
1.00
17.04
N


ATOM
709
CA
LYS
A
50
72.896
−49.226
−13.608
1.00
17.60
C


ATOM
711
CB
LYS
A
50
74.172
−49.859
−14.192
1.00
18.24
C


ATOM
714
CG
LYS
A
50
74.171
−50.048
−15.713
1.00
21.29
C


ATOM
717
CD
LYS
A
50
73.928
−51.487
−16.166
1.00
24.29
C


ATOM
720
CE
LYS
A
50
73.721
−51.580
−17.703
1.00
26.19
C


ATOM
723
NZ
LYS
A
50
72.596
−50.702
−18.227
1.00
22.19
N


ATOM
727
C
LYS
A
50
73.153
−48.790
−12.163
1.00
17.58
C


ATOM
728
O
LYS
A
50
73.908
−49.437
−11.442
1.00
18.10
O


ATOM
730
N
ALA
A
51
72.523
−47.704
−11.731
1.00
18.24
N


ATOM
731
CA
ALA
A
51
72.652
−47.252
−10.342
1.00
18.83
C


ATOM
733
CB
ALA
A
51
71.860
−45.964
−10.129
1.00
18.12
C


ATOM
737
C
ALA
A
51
72.177
−48.329
−9.358
1.00
19.47
C


ATOM
738
O
ALA
A
51
71.185
−49.015
−9.605
1.00
18.83
O


ATOM
740
N
GLU
A
52
72.896
−48.470
−8.249
1.00
20.59
N


ATOM
741
CA
GLU
A
52
72.472
−49.342
−7.152
1.00
21.65
C


ATOM
743
CB
GLU
A
52
73.602
−49.469
−6.120
1.00
23.29
C


ATOM
746
CG
GLU
A
52
73.756
−50.850
−5.504
1.00
30.52
C


ATOM
749
CD
GLU
A
52
74.671
−51.767
−6.316
1.00
39.49
C


ATOM
750
OE1
GLU
A
52
74.697
−51.642
−7.560
1.00
42.22
O


ATOM
751
OE2
GLU
A
52
75.362
−52.622
−5.709
1.00
45.07
O


ATOM
752
C
GLU
A
52
71.220
−48.724
−6.513
1.00
20.22
C


ATOM
753
O
GLU
A
52
71.126
−47.506
−6.397
1.00
20.92
O


ATOM
755
N
PHE
A
53
70.275
−49.557
−6.088
1.00
20.22
N


ATOM
756
CA
PHE
A
53
68.934
−49.085
−5.680
1.00
20.14
C


ATOM
758
CB
PHE
A
53
67.996
−50.258
−5.359
1.00
19.60
C


ATOM
761
CG
PHE
A
53
67.673
−51.137
−6.554
1.00
21.97
C


ATOM
762
CD1
PHE
A
53
67.408
−50.586
−7.807
1.00
20.30
C


ATOM
764
CE1
PHE
A
53
67.111
−51.395
−8.894
1.00
20.87
C


ATOM
766
CZ
PHE
A
53
67.060
−52.770
−8.741
1.00
22.56
C


ATOM
768
CE2
PHE
A
53
67.313
−53.336
−7.498
1.00
21.00
C


ATOM
770
CD2
PHE
A
53
67.616
−52.522
−6.414
1.00
20.77
C


ATOM
772
C
PHE
A
53
68.928
−48.104
−4.513
1.00
20.34
C


ATOM
773
O
PHE
A
53
68.429
−46.997
−4.651
1.00
20.72
O


ATOM
775
N
LEU
A
54
69.476
−48.502
−3.369
1.00
20.88
N


ATOM
776
CA
LEU
A
54
69.530
−47.612
−2.206
1.00
20.79
C


ATOM
778
CB
LEU
A
54
70.065
−48.344
−0.973
1.00
21.67
C


ATOM
781
CG
LEU
A
54
69.308
−49.602
−0.514
1.00
25.55
C


ATOM
783
CD1
LEU
A
54
69.953
−50.193
0.747
1.00
26.34
C


ATOM
787
CD2
LEU
A
54
67.820
−49.318
−0.285
1.00
21.98
C


ATOM
791
C
LEU
A
54
70.387
−46.381
−2.488
1.00
19.98
C


ATOM
792
O
LEU
A
54
70.029
−45.273
−2.103
1.00
21.36
O


ATOM
794
N
THR
A
55
71.508
−46.569
−3.171
1.00
18.78
N


ATOM
795
CA
THR
A
55
72.389
−45.449
−3.516
1.00
18.31
C


ATOM
797
CB
THR
A
55
73.642
−45.948
−4.244
1.00
17.70
C


ATOM
799
OG1
THR
A
55
74.213
−47.011
−3.483
1.00
19.06
O


ATOM
801
CG2
THR
A
55
74.666
−44.843
−4.394
1.00
15.55
C


ATOM
805
C
THR
A
55
71.675
−44.386
−4.364
1.00
17.24
C


ATOM
806
O
THR
A
55
71.851
−43.181
−4.141
1.00
15.19
O


ATOM
808
N
LEU
A
56
70.866
−44.849
−5.316
1.00
16.83
N


ATOM
809
CA
LEU
A
56
70.043
−43.973
−6.140
1.00
16.53
C


ATOM
811
CB
LEU
A
56
69.344
−44.783
−7.243
1.00
16.93
C


ATOM
814
CG
LEU
A
56
68.366
−44.034
−8.166
1.00
16.70
C


ATOM
816
CD1
LEU
A
56
69.015
−42.788
−8.765
1.00
14.20
C


ATOM
820
CD2
LEU
A
56
67.870
−44.958
−9.264
1.00
16.46
C


ATOM
824
C
LEU
A
56
69.005
−43.216
−5.316
1.00
16.09
C


ATOM
825
O
LEU
A
56
68.849
−42.011
−5.471
1.00
16.47
O


ATOM
827
N
LEU
A
57
68.289
−43.923
−4.449
1.00
15.87
N


ATOM
828
CA
LEU
A
57
67.250
−43.294
−3.634
1.00
15.86
C


ATOM
830
CB
LEU
A
57
66.493
−44.330
−2.792
1.00
15.00
C


ATOM
833
CG
LEU
A
57
65.729
−45.421
−3.558
1.00
15.34
C


ATOM
835
CD1
LEU
A
57
65.235
−46.483
−2.586
1.00
12.24
C


ATOM
839
CD2
LEU
A
57
64.570
−44.873
−4.400
1.00
5.90
C


ATOM
843
C
LEU
A
57
67.853
−42.215
−2.737
1.00
16.86
C


ATOM
844
O
LEU
A
57
67.292
−41.123
−2.608
1.00
17.20
O


ATOM
846
N
GLU
A
58
69.008
−42.509
−2.140
1.00
17.19
N


ATOM
847
CA
GLU
A
58
69.682
−41.542
−1.272
1.00
18.11
C


ATOM
849
CB
GLU
A
58
70.804
−42.209
−0.463
1.00
18.65
C


ATOM
852
CG
GLU
A
58
70.276
−43.185
0.603
1.00
24.37
C


ATOM
855
CD
GLU
A
58
71.362
−43.844
1.450
1.00
30.28
C


ATOM
856
OE1
GLU
A
58
72.569
−43.605
1.214
1.00
37.36
O


ATOM
857
OE2
GLU
A
58
70.997
−44.607
2.368
1.00
33.82
O


ATOM
858
C
GLU
A
58
70.200
−40.324
−2.042
1.00
18.11
C


ATOM
859
O
GLU
A
58
70.117
−39.200
−1.533
1.00
19.13
O


ATOM
861
N
LEU
A
59
70.716
−40.532
−3.258
1.00
17.41
N


ATOM
862
CA
LEU
A
59
71.157
−39.418
−4.114
1.00
15.69
C


ATOM
864
CB
LEU
A
59
71.742
−39.923
−5.444
1.00
15.51
C


ATOM
867
CG
LEU
A
59
72.022
−38.864
−6.532
1.00
16.03
C


ATOM
869
CD1
LEU
A
59
73.046
−37.855
−6.046
1.00
8.96
C


ATOM
873
CD2
LEU
A
59
72.455
−39.485
−7.871
1.00
10.61
C


ATOM
877
C
LEU
A
59
69.985
−38.487
−4.374
1.00
15.15
C


ATOM
878
O
LEU
A
59
70.112
−37.274
−4.248
1.00
16.65
O


ATOM
880
N
ILE
A
60
68.833
−39.061
−4.711
1.00
14.37
N


ATOM
881
CA
ILE
A
60
67.636
−38.270
−4.976
1.00
13.67
C


ATOM
883
CB
ILE
A
60
66.475
−39.137
−5.492
1.00
13.84
C


ATOM
885
CG1
ILE
A
60
66.815
−39.738
−6.857
1.00
11.78
C


ATOM
888
CD1
ILE
A
60
65.883
−40.833
−7.294
1.00
13.41
C


ATOM
892
CG2
ILE
A
60
65.210
−38.305
−5.607
1.00
12.65
C


ATOM
896
C
ILE
A
60
67.208
−37.544
−3.710
1.00
13.90
C


ATOM
897
O
ILE
A
60
66.960
−36.345
−3.730
1.00
14.71
O


ATOM
899
N
ASP
A
61
67.152
−38.269
−2.601
1.00
14.05
N


ATOM
900
CA
ASP
A
61
66.865
−37.652
−1.310
1.00
14.37
C


ATOM
902
CB
ASP
A
61
66.992
−38.685
−0.196
1.00
15.27
C


ATOM
905
CG
ASP
A
61
66.334
−38.249
1.077
1.00
15.09
C


ATOM
906
OD1
ASP
A
61
65.373
−37.449
1.015
1.00
18.28
O


ATOM
907
OD2
ASP
A
61
66.764
−38.731
2.144
1.00
19.88
O


ATOM
908
C
ASP
A
61
67.786
−36.465
−1.010
1.00
13.95
C


ATOM
909
O
ASP
A
61
67.312
−35.376
−0.700
1.00
14.30
O


ATOM
911
N
ASN
A
62
69.095
−36.673
−1.111
1.00
13.73
N


ATOM
912
CA
ASN
A
62
70.056
−35.601
−0.859
1.00
14.39
C


ATOM
914
CB
ASN
A
62
71.498
−36.078
−1.056
1.00
15.08
C


ATOM
917
CG
ASN
A
62
71.973
−37.004
0.039
1.00
14.59
C


ATOM
918
OD1
ASN
A
62
71.511
−36.934
1.165
1.00
15.77
O


ATOM
919
ND2
ASN
A
62
72.919
−37.871
−0.292
1.00
16.01
N


ATOM
922
C
ASN
A
62
69.811
−34.415
−1.776
1.00
14.95
C


ATOM
923
O
ASN
A
62
69.708
−33.282
−1.313
1.00
16.30
O


ATOM
925
N
VAL
A
63
69.707
−34.681
−3.077
1.00
14.64
N


ATOM
926
CA
VAL
A
63
69.485
−33.621
−4.054
1.00
14.05
C


ATOM
928
CB
VAL
A
63
69.239
−34.185
−5.464
1.00
13.98
C


ATOM
930
CG1
VAL
A
63
68.736
−33.090
−6.399
1.00
11.96
C


ATOM
934
CG2
VAL
A
63
70.511
−34.805
−6.016
1.00
11.21
C


ATOM
938
C
VAL
A
63
68.297
−32.758
−3.633
1.00
14.72
C


ATOM
939
O
VAL
A
63
68.385
−31.525
−3.603
1.00
14.02
O


ATOM
941
N
GLN
A
64
67.197
−33.415
−3.288
1.00
14.83
N


ATOM
942
CA
GLN
A
64
65.991
−32.703
−2.892
1.00
16.08
C


ATOM
944
CB
GLN
A
64
64.807
−33.675
−2.766
1.00
15.92
C


ATOM
947
CG
GLN
A
64
64.437
−34.322
−4.099
1.00
13.35
C


ATOM
950
CD
GLN
A
64
63.079
−34.965
−4.090
1.00
12.31
C


ATOM
951
OE1
GLN
A
64
62.366
−34.925
−5.091
1.00
16.14
O


ATOM
952
NE2
GLN
A
64
62.704
−35.559
−2.958
1.00
9.25
N


ATOM
955
C
GLN
A
64
66.205
−31.929
−1.595
1.00
16.42
C


ATOM
956
O
GLN
A
64
65.885
−30.743
−1.517
1.00
15.20
O


ATOM
958
N
ARG
A
65
66.768
−32.599
−0.593
1.00
17.50
N


ATOM
959
CA
ARG
A
65
66.913
−32.006
0.739
1.00
17.71
C


ATOM
961
CB
ARG
A
65
67.418
−33.041
1.757
1.00
17.94
C


ATOM
964
CG
ARG
A
65
66.368
−34.108
2.094
1.00
18.27
C


ATOM
967
CD
ARG
A
65
66.709
−34.903
3.331
1.00
19.29
C


ATOM
970
NE
ARG
A
65
66.763
−34.043
4.501
1.00
25.35
N


ATOM
972
CZ
ARG
A
65
67.051
−34.456
5.732
1.00
29.90
C


ATOM
973
NH1
ARG
A
65
67.298
−35.737
5.976
1.00
28.26
N


ATOM
976
NH2
ARG
A
65
67.086
−33.576
6.730
1.00
33.55
N


ATOM
979
C
ARG
A
65
67.827
−30.798
0.702
1.00
16.77
C


ATOM
980
O
ARG
A
65
67.580
−29.813
1.389
1.00
17.22
O


ATOM
982
N
LEU
A
66
68.858
−30.867
−0.136
1.00
16.24
N


ATOM
983
CA
LEU
A
66
69.815
−29.773
−0.285
1.00
14.99
C


ATOM
985
CB
LEU
A
66
71.091
−30.282
−0.950
1.00
14.29
C


ATOM
988
CG
LEU
A
66
71.926
−31.262
−0.120
1.00
14.21
C


ATOM
990
CD1
LEU
A
66
72.898
−32.029
−1.013
1.00
12.16
C


ATOM
994
CD2
LEU
A
66
72.694
−30.519
0.985
1.00
12.46
C


ATOM
998
C
LEU
A
66
69.244
−28.603
−1.085
1.00
15.42
C


ATOM
999
O
LEU
A
66
69.945
−27.617
−1.307
1.00
15.95
O


ATOM
1001
N
GLY
A
67
67.990
−28.725
−1.530
1.00
15.16
N


ATOM
1002
CA
GLY
A
67
67.254
−27.625
−2.151
1.00
14.99
C


ATOM
1005
C
GLY
A
67
67.272
−27.602
−3.668
1.00
15.18
C


ATOM
1006
O
GLY
A
67
66.767
−26.657
−4.268
1.00
16.15
O


ATOM
1008
N
LEU
A
68
67.829
−28.644
−4.288
1.00
14.91
N


ATOM
1009
CA
LEU
A
68
68.013
−28.703
−5.745
1.00
14.20
C


ATOM
1011
CB
LEU
A
68
69.394
−29.293
−6.074
1.00
13.73
C


ATOM
1014
CG
LEU
A
68
70.598
−28.464
−5.621
1.00
12.88
C


ATOM
1016
CD1
LEU
A
68
71.849
−29.339
−5.475
1.00
8.92
C


ATOM
1020
CD2
LEU
A
68
70.858
−27.277
−6.568
1.00
8.96
C


ATOM
1024
C
LEU
A
68
66.933
−29.502
−6.488
1.00
14.67
C


ATOM
1025
O
LEU
A
68
66.989
−29.619
−7.711
1.00
14.49
O


ATOM
1027
N
GLY
A
69
65.956
−30.047
−5.763
1.00
15.47
N


ATOM
1028
CA
GLY
A
69
64.867
−30.817
−6.374
1.00
14.82
C


ATOM
1031
C
GLY
A
69
64.230
−30.163
−7.592
1.00
15.77
C


ATOM
1032
O
GLY
A
69
64.091
−30.794
−8.650
1.00
15.84
O


ATOM
1034
N
TYR
A
70
63.861
−28.889
−7.451
1.00
16.45
N


ATOM
1035
CA
TYR
A
70
63.181
−28.131
−8.521
1.00
16.04
C


ATOM
1037
CB
TYR
A
70
62.941
−26.686
−8.080
1.00
15.12
C


ATOM
1040
CG
TYR
A
70
64.159
−25.789
−8.181
1.00
14.52
C


ATOM
1041
CD1
TYR
A
70
65.151
−25.816
−7.210
1.00
12.49
C


ATOM
1043
CE1
TYR
A
70
66.277
−24.998
−7.303
1.00
13.94
C


ATOM
1045
CZ
TYR
A
70
66.405
−24.132
−8.371
1.00
16.38
C


ATOM
1046
OH
TYR
A
70
67.512
−23.315
−8.466
1.00
14.89
O


ATOM
1048
CE2
TYR
A
70
65.425
−24.088
−9.353
1.00
14.01
C


ATOM
1050
CD2
TYR
A
70
64.314
−24.910
−9.254
1.00
11.99
C


ATOM
1052
C
TYR
A
70
63.955
−28.131
−9.835
1.00
18.04
C


ATOM
1053
O
TYR
A
70
63.385
−27.959
−10.913
1.00
20.46
O


ATOM
1055
N
ARG
A
71
65.258
−28.327
−9.732
1.00
18.33
N


ATOM
1056
CA
ARG
A
71
66.156
−28.251
−10.873
1.00
18.97
C


ATOM
1058
CB
ARG
A
71
67.521
−27.791
−10.352
1.00
19.28
C


ATOM
1061
CG
ARG
A
71
68.522
−27.420
−11.395
1.00
20.34
C


ATOM
1064
CD
ARG
A
71
69.657
−26.634
−10.770
1.00
19.82
C


ATOM
1067
NE
ARG
A
71
69.326
−25.224
−10.689
1.00
21.37
N


ATOM
1069
CZ
ARG
A
71
69.326
−24.404
−11.732
1.00
23.52
C


ATOM
1070
NH1
ARG
A
71
69.009
−23.138
−11.563
1.00
27.16
N


ATOM
1073
NH2
ARG
A
71
69.643
−24.842
−12.944
1.00
24.15
N


ATOM
1076
C
ARG
A
71
66.289
−29.586
−11.615
1.00
18.48
C


ATOM
1077
O
ARG
A
71
66.647
−29.601
−12.790
1.00
18.00
O


ATOM
1079
N
PHE
A
72
66.002
−30.691
−10.925
1.00
18.29
N


ATOM
1080
CA
PHE
A
72
66.179
−32.039
−11.465
1.00
18.27
C


ATOM
1082
CB
PHE
A
72
67.269
−32.777
−10.667
1.00
18.42
C


ATOM
1085
CG
PHE
A
72
68.626
−32.144
−10.767
1.00
16.33
C


ATOM
1086
CD1
PHE
A
72
69.394
−32.302
−11.907
1.00
11.77
C


ATOM
1088
CE1
PHE
A
72
70.641
−31.707
−12.007
1.00
16.47
C


ATOM
1090
CZ
PHE
A
72
71.136
−30.940
−10.954
1.00
15.78
C


ATOM
1092
CE2
PHE
A
72
70.383
−30.777
−9.821
1.00
15.51
C


ATOM
1094
CD2
PHE
A
72
69.129
−31.376
−9.729
1.00
16.02
C


ATOM
1096
C
PHE
A
72
64.881
−32.862
−11.451
1.00
18.90
C


ATOM
1097
O
PHE
A
72
64.910
−34.093
−11.493
1.00
17.74
O


ATOM
1099
N
GLU
A
73
63.740
−32.188
−11.426
1.00
20.36
N


ATOM
1100
CA
GLU
A
73
62.466
−32.882
−11.261
1.00
21.99
C


ATOM
1102
CB
GLU
A
73
61.307
−31.884
−11.219
1.00
22.99
C


ATOM
1105
CG
GLU
A
73
59.995
−32.529
−10.830
1.00
29.40
C


ATOM
1108
CD
GLU
A
73
58.845
−31.560
−10.766
1.00
35.93
C


ATOM
1109
OE1
GLU
A
73
59.091
−30.335
−10.727
1.00
42.73
O


ATOM
1110
OE2
GLU
A
73
57.690
−32.036
−10.751
1.00
39.11
O


ATOM
1111
C
GLU
A
73
62.201
−33.955
−12.323
1.00
21.43
C


ATOM
1112
O
GLU
A
73
61.886
−35.093
−11.987
1.00
21.47
O


ATOM
1114
N
SER
A
74
62.318
−33.606
−13.598
1.00
21.46
N


ATOM
1115
CA
SER
A
74
62.074
−34.594
−14.649
1.00
21.83
C


ATOM
1117
CB
SER
A
74
62.037
−33.936
−16.024
1.00
22.03
C


ATOM
1120
OG
SER
A
74
63.243
−33.257
−16.273
1.00
27.93
O


ATOM
1122
C
SER
A
74
63.121
−35.707
−14.615
1.00
20.53
C


ATOM
1123
O
SER
A
74
62.792
−36.883
−14.794
1.00
20.23
O


ATOM
1125
N
ASP
A
75
64.378
−35.333
−14.383
1.00
19.65
N


ATOM
1126
CA
ASP
A
75
65.449
−36.315
−14.220
1.00
18.66
C


ATOM
1128
CB
ASP
A
75
66.790
−35.625
−13.949
1.00
18.80
C


ATOM
1131
CG
ASP
A
75
67.381
−34.969
−15.191
1.00
19.71
C


ATOM
1132
OD1
ASP
A
75
67.482
−35.634
−16.240
1.00
18.92
O


ATOM
1133
OD2
ASP
A
75
67.766
−33.786
−15.111
1.00
23.76
O


ATOM
1134
C
ASP
A
75
65.114
−37.274
−13.083
1.00
17.66
C


ATOM
1135
O
ASP
A
75
65.232
−38.486
−13.230
1.00
17.70
O


ATOM
1137
N
ILE
A
76
64.678
−36.722
−11.954
1.00
17.25
N


ATOM
1138
CA
ILE
A
76
64.221
−37.523
−10.820
1.00
16.28
C


ATOM
1140
CB
ILE
A
76
63.938
−36.633
−9.594
1.00
16.35
C


ATOM
1142
CG1
ILE
A
76
65.258
−36.073
−9.040
1.00
15.94
C


ATOM
1145
CD1
ILE
A
76
65.092
−34.884
−8.092
1.00
11.79
C


ATOM
1149
CG2
ILE
A
76
63.211
−37.426
−8.510
1.00
13.58
C


ATOM
1153
C
ILE
A
76
62.986
−38.386
−11.143
1.00
17.08
C


ATOM
1154
O
ILE
A
76
62.922
−39.539
−10.713
1.00
17.23
O


ATOM
1156
N
ARG
A
77
62.018
−37.856
−11.898
1.00
17.36
N


ATOM
1157
CA
ARG
A
77
60.833
−38.650
−12.271
1.00
18.20
C


ATOM
1159
CB
ARG
A
77
59.809
−37.829
−13.054
1.00
19.55
C


ATOM
1162
CG
ARG
A
77
59.093
−36.786
−12.211
1.00
30.35
C


ATOM
1165
CD
ARG
A
77
58.514
−35.644
−13.053
1.00
39.84
C


ATOM
1168
NE
ARG
A
77
57.449
−36.103
−13.934
1.00
48.68
N


ATOM
1170
CZ
ARG
A
77
56.226
−36.450
−13.531
1.00
59.20
C


ATOM
1171
NH1
ARG
A
77
55.887
−36.403
−12.241
1.00
61.69
N


ATOM
1174
NH2
ARG
A
77
55.326
−36.852
−14.426
1.00
62.52
N


ATOM
1177
C
ARG
A
77
61.240
−39.866
−13.081
1.00
17.19
C


ATOM
1178
O
ARG
A
77
60.773
−40.975
−12.819
1.00
17.85
O


ATOM
1180
N
GLY
A
78
62.119
−39.659
−14.056
1.00
15.51
N


ATOM
1181
CA
GLY
A
78
62.591
−40.742
−14.899
1.00
14.70
C


ATOM
1184
C
GLY
A
78
63.378
−41.766
−14.113
1.00
15.16
C


ATOM
1185
O
GLY
A
78
63.211
−42.967
−14.316
1.00
17.14
O


ATOM
1187
N
ALA
A
79
64.239
−41.301
−13.212
1.00
14.47
N


ATOM
1188
CA
ALA
A
79
65.013
−42.204
−12.368
1.00
14.61
C


ATOM
1190
CB
ALA
A
79
65.937
−41.425
−11.455
1.00
13.92
C


ATOM
1194
C
ALA
A
79
64.089
−43.105
−11.551
1.00
15.53
C


ATOM
1195
O
ALA
A
79
64.291
−44.312
−11.484
1.00
16.20
O


ATOM
1197
N
LEU
A
80
63.067
−42.509
−10.947
1.00
17.11
N


ATOM
1198
CA
LEU
A
80
62.078
−43.259
−10.188
1.00
18.35
C


ATOM
1200
CB
LEU
A
80
61.165
−42.304
−9.408
1.00
19.36
C


ATOM
1203
CG
LEU
A
80
61.820
−41.589
−8.218
1.00
18.62
C


ATOM
1205
CD1
LEU
A
80
60.915
−40.475
−7.707
1.00
15.51
C


ATOM
1209
CD2
LEU
A
80
62.176
−42.587
−7.097
1.00
12.92
C


ATOM
1213
C
LEU
A
80
61.244
−44.178
−11.082
1.00
19.46
C


ATOM
1214
O
LEU
A
80
60.869
−45.262
−10.661
1.00
20.30
O


ATOM
1216
N
ASP
A
81
60.960
−43.754
−12.308
1.00
20.42
N


ATOM
1217
CA
ASP
A
81
60.266
−44.616
−13.263
1.00
22.07
C


ATOM
1219
CB
ASP
A
81
59.981
−43.877
−14.576
1.00
23.40
C


ATOM
1222
CG
ASP
A
81
58.910
−44.558
−15.419
1.00
27.38
C


ATOM
1223
OD1
ASP
A
81
57.775
−44.718
−14.925
1.00
35.42
O


ATOM
1224
OD2
ASP
A
81
59.193
−44.912
−16.583
1.00
33.33
O


ATOM
1225
C
ASP
A
81
61.093
−45.862
−13.544
1.00
22.17
C


ATOM
1226
O
ASP
A
81
60.561
−46.971
−13.568
1.00
23.66
O


ATOM
1228
N
ARG
A
82
62.393
−45.681
−13.750
1.00
22.55
N


ATOM
1229
CA
ARG
A
82
63.291
−46.817
−13.984
1.00
23.77
C


ATOM
1231
CB
ARG
A
82
64.667
−46.337
−14.463
1.00
25.05
C


ATOM
1234
CG
ARG
A
82
65.692
−47.463
−14.709
1.00
27.16
C


ATOM
1237
CD
ARG
A
82
67.027
−46.899
−15.176
1.00
28.82
C


ATOM
1240
NE
ARG
A
82
66.881
−46.132
−16.416
1.00
30.19
N


ATOM
1242
CZ
ARG
A
82
67.011
−46.623
−17.654
1.00
31.69
C


ATOM
1243
NH1
ARG
A
82
67.310
−47.905
−17.872
1.00
27.72
N


ATOM
1246
NH2
ARG
A
82
66.845
−45.812
−18.694
1.00
32.14
N


ATOM
1249
C
ARG
A
82
63.443
−47.680
−12.728
1.00
23.10
C


ATOM
1250
O
ARG
A
82
63.508
−48.903
−12.815
1.00
23.80
O


ATOM
1252
N
PHE
A
83
63.504
−47.033
−11.567
1.00
21.87
N


ATOM
1253
CA
PHE
A
83
63.604
−47.734
−10.302
1.00
20.68
C


ATOM
1255
CB
PHE
A
83
63.547
−46.722
−9.145
1.00
20.79
C


ATOM
1258
CG
PHE
A
83
63.463
−47.349
−7.779
1.00
18.77
C


ATOM
1259
CD1
PHE
A
83
64.586
−47.868
−7.175
1.00
18.63
C


ATOM
1261
CE1
PHE
A
83
64.513
−48.454
−5.918
1.00
21.29
C


ATOM
1263
CZ
PHE
A
83
63.302
−48.514
−5.247
1.00
21.04
C


ATOM
1265
CE2
PHE
A
83
62.166
−47.989
−5.841
1.00
22.98
C


ATOM
1267
CD2
PHE
A
83
62.253
−47.409
−7.101
1.00
20.98
C


ATOM
1269
C
PHE
A
83
62.499
−48.787
−10.198
1.00
21.01
C


ATOM
1270
O
PHE
A
83
62.756
−49.926
−9.820
1.00
21.25
O


ATOM
1272
N
VAL
A
84
61.276
−48.413
−10.565
1.00
22.16
N


ATOM
1273
CA
VAL
A
84
60.132
−49.323
−10.463
1.00
22.20
C


ATOM
1275
CB
VAL
A
84
58.785
−48.578
−10.590
1.00
22.42
C


ATOM
1277
CG1
VAL
A
84
57.626
−49.567
−10.704
1.00
17.38
C


ATOM
1281
CG2
VAL
A
84
58.587
−47.644
−9.400
1.00
19.99
C


ATOM
1285
C
VAL
A
84
60.207
−50.394
−11.528
1.00
23.13
C


ATOM
1286
O
VAL
A
84
60.197
−51.581
−11.212
1.00
23.07
O


ATOM
1288
N
SER
A
85
60.320
−49.966
−12.784
1.00
24.55
N


ATOM
1289
CA
SER
A
85
60.202
−50.883
−13.920
1.00
25.78
C


ATOM
1291
CB
SER
A
85
60.133
−50.116
−15.244
1.00
26.02
C


ATOM
1294
OG
SER
A
85
61.407
−49.643
−15.636
1.00
29.26
O


ATOM
1296
C
SER
A
85
61.324
−51.917
−13.969
1.00
25.92
C


ATOM
1297
O
SER
A
85
61.161
−52.969
−14.583
1.00
26.29
O


ATOM
1299
N
SER
A
86
62.451
−51.621
−13.320
1.00
26.24
N


ATOM
1300
CA
SER
A
86
63.561
−52.573
−13.221
1.00
26.71
C


ATOM
1302
CB
SER
A
86
64.903
−51.845
−13.353
1.00
26.89
C


ATOM
1305
OG
SER
A
86
65.247
−51.174
−12.156
1.00
27.56
O


ATOM
1307
C
SER
A
86
63.535
−53.394
−11.923
1.00
26.91
C


ATOM
1308
O
SER
A
86
64.525
−54.046
−11.579
1.00
26.48
O


ATOM
1310
N
GLY
A
87
62.409
−53.359
−11.210
1.00
27.49
N


ATOM
1311
CA
GLY
A
87
62.201
−54.201
−10.031
1.00
27.23
C


ATOM
1314
C
GLY
A
87
62.861
−53.717
−8.754
1.00
26.76
C


ATOM
1315
O
GLY
A
87
62.991
−54.476
−7.798
1.00
27.05
O


ATOM
1317
N
GLY
A
88
63.272
−52.455
−8.727
1.00
26.57
N


ATOM
1318
CA
GLY
A
88
63.930
−51.891
−7.555
1.00
26.30
C


ATOM
1321
C
GLY
A
88
62.978
−51.772
−6.384
1.00
26.50
C


ATOM
1322
O
GLY
A
88
63.340
−52.072
−5.244
1.00
26.40
O


ATOM
1324
N
PHE
A
89
61.752
−51.342
−6.666
1.00
26.08
N


ATOM
1325
CA
PHE
A
89
60.745
−51.205
−5.628
1.00
26.31
C


ATOM
1327
CB
PHE
A
89
59.531
−50.444
−6.153
1.00
25.89
C


ATOM
1330
CG
PHE
A
89
58.544
−50.075
−5.085
1.00
23.78
C


ATOM
1331
CD1
PHE
A
89
58.946
−49.360
−3.967
1.00
23.52
C


ATOM
1333
CE1
PHE
A
89
58.044
−49.015
−2.985
1.00
22.50
C


ATOM
1335
CZ
PHE
A
89
56.724
−49.375
−3.110
1.00
23.15
C


ATOM
1337
CE2
PHE
A
89
56.308
−50.081
−4.214
1.00
24.72
C


ATOM
1339
CD2
PHE
A
89
57.216
−50.429
−5.199
1.00
23.79
C


ATOM
1341
C
PHE
A
89
60.315
−52.564
−5.097
1.00
27.43
C


ATOM
1342
O
PHE
A
89
60.014
−52.712
−3.916
1.00
27.98
O


ATOM
1344
N
ASP
A
90
60.282
−53.549
−5.984
1.00
28.22
N


ATOM
1345
CA
ASP
A
90
60.010
−54.916
−5.598
1.00
28.96
C


ATOM
1347
CB
ASP
A
90
59.849
−55.805
−6.835
1.00
30.61
C


ATOM
1350
CG
ASP
A
90
58.799
−56.871
−6.645
1.00
35.50
C


ATOM
1351
OD1
ASP
A
90
57.601
−56.517
−6.719
1.00
42.54
O


ATOM
1352
OD2
ASP
A
90
59.167
−58.048
−6.425
1.00
37.40
O


ATOM
1353
C
ASP
A
90
61.162
−55.420
−4.744
1.00
28.08
C


ATOM
1354
O
ASP
A
90
60.949
−56.017
−3.698
1.00
27.56
O


ATOM
1356
N
ALA
A
91
62.388
−55.165
−5.189
1.00
27.90
N


ATOM
1357
CA
ALA
A
91
63.570
−55.562
−4.425
1.00
28.29
C


ATOM
1359
CB
ALA
A
91
64.843
−55.090
−5.125
1.00
28.02
C


ATOM
1363
C
ALA
A
91
63.530
−55.041
−2.981
1.00
27.99
C


ATOM
1364
O
ALA
A
91
63.595
−55.823
−2.037
1.00
28.71
O


ATOM
1366
N
VAL
A
92
63.409
−53.727
−2.819
1.00
27.52
N


ATOM
1367
CA
VAL
A
92
63.439
−53.115
−1.491
1.00
27.31
C


ATOM
1369
CB
VAL
A
92
63.424
−51.558
−1.541
1.00
27.02
C


ATOM
1371
CG1
VAL
A
92
64.645
−51.030
−2.280
1.00
22.62
C


ATOM
1375
CG2
VAL
A
92
62.146
−51.048
−2.164
1.00
26.79
C


ATOM
1379
C
VAL
A
92
62.294
−53.589
−0.602
1.00
27.90
C


ATOM
1380
O
VAL
A
92
62.497
−53.821
0.590
1.00
28.35
O


ATOM
1382
N
THR
A
93
61.104
−53.760
−1.170
1.00
28.23
N


ATOM
1383
CA
THR
A
93
59.960
−54.211
−0.370
1.00
29.05
C


ATOM
1385
CB
THR
A
93
58.649
−54.319
−1.187
1.00
29.02
C


ATOM
1387
OG1
THR
A
93
58.818
−55.245
−2.266
1.00
28.86
O


ATOM
1389
CG2
THR
A
93
58.222
−52.950
−1.721
1.00
29.04
C


ATOM
1393
C
THR
A
93
60.216
−55.550
0.317
1.00
29.41
C


ATOM
1394
O
THR
A
93
59.603
−55.830
1.350
1.00
30.18
O


ATOM
1396
N
LYS
A
94
61.117
−56.359
−0.248
1.00
29.78
N


ATOM
1397
CA
LYS
A
94
61.424
−57.697
0.274
1.00
30.05
C


ATOM
1399
CB
LYS
A
94
61.405
−58.728
−0.869
1.00
30.16
C


ATOM
1402
CG
LYS
A
94
60.132
−58.717
−1.748
1.00
34.75
C


ATOM
1405
CD
LYS
A
94
58.824
−58.774
−0.928
1.00
41.79
C


ATOM
1408
CE
LYS
A
94
57.580
−58.482
−1.788
1.00
47.02
C


ATOM
1411
NZ
LYS
A
94
56.435
−57.895
−1.002
1.00
45.97
N


ATOM
1415
C
LYS
A
94
62.762
−57.786
1.015
1.00
29.38
C


ATOM
1416
O
LYS
A
94
63.217
−58.882
1.317
1.00
30.59
O


ATOM
1418
N
THR
A
95
63.382
−56.651
1.331
1.00
28.34
N


ATOM
1419
CA
THR
A
95
64.706
−56.656
1.973
1.00
27.18
C


ATOM
1421
CB
THR
A
95
65.834
−56.656
0.918
1.00
27.59
C


ATOM
1423
OG1
THR
A
95
65.532
−55.696
−0.105
1.00
27.34
O


ATOM
1425
CG2
THR
A
95
66.004
−58.039
0.291
1.00
26.29
C


ATOM
1429
C
THR
A
95
64.987
−55.478
2.911
1.00
26.86
C


ATOM
1430
O
THR
A
95
65.660
−55.656
3.924
1.00
26.77
O


ATOM
1432
N
SER
A
96
64.493
−54.284
2.572
1.00
26.14
N


ATOM
1433
CA
SER
A
96
64.907
−53.049
3.248
1.00
24.86
C


ATOM
1435
CB
SER
A
96
65.881
−52.280
2.352
1.00
24.50
C


ATOM
1438
OG
SER
A
96
66.217
−51.027
2.923
1.00
24.84
O


ATOM
1440
C
SER
A
96
63.741
−52.135
3.612
1.00
24.07
C


ATOM
1441
O
SER
A
96
63.058
−51.607
2.726
1.00
23.24
O


ATOM
1443
N
LEU
A
97
63.531
−51.933
4.916
1.00
23.40
N


ATOM
1444
CA
LEU
A
97
62.540
−50.963
5.394
1.00
22.78
C


ATOM
1446
CB
LEU
A
97
62.418
−50.978
6.924
1.00
23.20
C


ATOM
1449
CG
LEU
A
97
61.506
−49.892
7.521
1.00
22.25
C


ATOM
1451
CD1
LEU
A
97
60.078
−50.016
6.988
1.00
19.42
C


ATOM
1455
CD2
LEU
A
97
61.505
−49.949
9.035
1.00
22.20
C


ATOM
1459
C
LEU
A
97
62.958
−49.580
4.955
1.00
21.37
C


ATOM
1460
O
LEU
A
97
62.136
−48.800
4.462
1.00
21.91
O


ATOM
1462
N
HIS
A
98
64.241
−49.281
5.145
1.00
19.60
N


ATOM
1463
CA
HIS
A
98
64.787
−47.994
4.741
1.00
19.79
C


ATOM
1465
CB
HIS
A
98
66.300
−47.943
4.974
1.00
19.76
C


ATOM
1468
CG
HIS
A
98
66.939
−46.689
4.467
1.00
22.66
C


ATOM
1469
ND1
HIS
A
98
66.379
−45.443
4.656
1.00
23.03
N


ATOM
1471
CE1
HIS
A
98
67.149
−44.529
4.096
1.00
23.75
C


ATOM
1473
NE2
HIS
A
98
68.190
−45.135
3.554
1.00
25.40
N


ATOM
1475
CD2
HIS
A
98
68.083
−46.486
3.774
1.00
25.37
C


ATOM
1477
C
HIS
A
98
64.452
−47.701
3.276
1.00
18.86
C


ATOM
1478
O
HIS
A
98
63.821
−46.687
2.968
1.00
16.70
O


ATOM
1480
N
GLY
A
99
64.858
−48.607
2.388
1.00
18.34
N


ATOM
1481
CA
GLY
A
99
64.601
−48.453
0.956
1.00
17.49
C


ATOM
1484
C
GLY
A
99
63.128
−48.291
0.648
1.00
17.45
C


ATOM
1485
O
GLY
A
99
62.745
−47.404
−0.117
1.00
16.89
O


ATOM
1487
N
THR
A
100
62.307
−49.145
1.265
1.00
17.77
N


ATOM
1488
CA
THR
A
100
60.853
−49.139
1.070
1.00
16.46
C


ATOM
1490
CB
THR
A
100
60.189
−50.304
1.838
1.00
16.82
C


ATOM
1492
OG1
THR
A
100
60.722
−51.549
1.362
1.00
16.05
O


ATOM
1494
CG2
THR
A
100
58.672
−50.301
1.664
1.00
12.76
C


ATOM
1498
C
THR
A
100
60.247
−47.808
1.502
1.00
16.62
C


ATOM
1499
O
THR
A
100
59.548
−47.158
0.725
1.00
18.20
O


ATOM
1501
N
ALA
A
101
60.531
−47.390
2.729
1.00
16.33
N


ATOM
1502
CA
ALA
A
101
60.011
−46.111
3.232
1.00
16.05
C


ATOM
1504
CB
ALA
A
101
60.475
−45.881
4.666
1.00
15.37
C


ATOM
1508
C
ALA
A
101
60.431
−44.936
2.337
1.00
15.29
C


ATOM
1509
O
ALA
A
101
59.605
−44.122
1.922
1.00
15.29
O


ATOM
1511
N
LEU
A
102
61.721
−44.867
2.034
1.00
15.18
N


ATOM
1512
CA
LEU
A
102
62.270
−43.768
1.254
1.00
15.07
C


ATOM
1514
CB
LEU
A
102
63.801
−43.856
1.224
1.00
14.12
C


ATOM
1517
CG
LEU
A
102
64.545
−42.770
0.448
1.00
12.87
C


ATOM
1519
CD1
LEU
A
102
64.090
−41.367
0.868
1.00
8.26
C


ATOM
1523
CD2
LEU
A
102
66.045
−42.944
0.631
1.00
5.88
C


ATOM
1527
C
LEU
A
102
61.692
−43.765
−0.160
1.00
15.44
C


ATOM
1528
O
LEU
A
102
61.268
−42.726
−0.666
1.00
15.75
O


ATOM
1530
N
SER
A
103
61.649
−44.935
−0.787
1.00
15.40
N


ATOM
1531
CA
SER
A
103
61.088
−45.041
−2.129
1.00
15.43
C


ATOM
1533
CB
SER
A
103
61.362
−46.424
−2.714
1.00
15.47
C


ATOM
1536
OG
SER
A
103
60.768
−47.430
−1.924
1.00
17.65
O


ATOM
1538
C
SER
A
103
59.589
−44.717
−2.141
1.00
15.00
C


ATOM
1539
O
SER
A
103
59.122
−43.981
−2.998
1.00
15.23
O


ATOM
1541
N
PHE
A
104
58.839
−45.253
−1.183
1.00
15.40
N


ATOM
1542
CA
PHE
A
104
57.417
−44.909
−1.037
1.00
15.09
C


ATOM
1544
CB
PHE
A
104
56.851
−45.462
0.279
1.00
15.48
C


ATOM
1547
CG
PHE
A
104
55.379
−45.221
0.447
1.00
13.12
C


ATOM
1548
CD1
PHE
A
104
54.908
−44.003
0.884
1.00
15.81
C


ATOM
1550
CE1
PHE
A
104
53.546
−43.777
1.022
1.00
19.45
C


ATOM
1552
CZ
PHE
A
104
52.642
−44.778
0.712
1.00
15.26
C


ATOM
1554
CE2
PHE
A
104
53.102
−45.988
0.270
1.00
12.72
C


ATOM
1556
CD2
PHE
A
104
54.464
−46.206
0.134
1.00
13.12
C


ATOM
1558
C
PHE
A
104
57.201
−43.399
−1.053
1.00
14.62
C


ATOM
1559
O
PHE
A
104
56.311
−42.888
−1.725
1.00
15.32
O


ATOM
1561
N
ARG
A
105
58.011
−42.698
−0.274
1.00
14.38
N


ATOM
1562
CA
ARG
A
105
57.886
−41.258
−0.132
1.00
14.61
C


ATOM
1564
CB
ARG
A
105
58.856
−40.764
0.946
1.00
14.63
C


ATOM
1567
CG
ARG
A
105
58.932
−39.258
1.101
1.00
14.01
C


ATOM
1570
CD
ARG
A
105
59.503
−38.878
2.476
1.00
13.58
C


ATOM
1573
NE
ARG
A
105
60.954
−39.024
2.549
1.00
13.53
N


ATOM
1575
CZ
ARG
A
105
61.829
−38.179
2.004
1.00
13.34
C


ATOM
1576
NH1
ARG
A
105
61.414
−37.118
1.317
1.00
16.92
N


ATOM
1579
NH2
ARG
A
105
63.130
−38.400
2.133
1.00
14.85
N


ATOM
1582
C
ARG
A
105
58.173
−40.585
−1.470
1.00
14.87
C


ATOM
1583
O
ARG
A
105
57.314
−39.910
−2.026
1.00
14.73
O


ATOM
1585
N
LEU
A
106
59.379
−40.809
−1.983
1.00
14.52
N


ATOM
1586
CA
LEU
A
106
59.816
−40.217
−3.231
1.00
13.90
C


ATOM
1588
CB
LEU
A
106
61.226
−40.709
−3.575
1.00
14.07
C


ATOM
1591
CG
LEU
A
106
62.348
−40.279
−2.620
1.00
13.34
C


ATOM
1593
CD1
LEU
A
106
63.702
−40.880
−3.034
1.00
8.71
C


ATOM
1597
CD2
LEU
A
106
62.435
−38.759
−2.549
1.00
9.95
C


ATOM
1601
C
LEU
A
106
58.850
−40.525
−4.379
1.00
13.52
C


ATOM
1602
O
LEU
A
106
58.539
−39.651
−5.179
1.00
12.39
O


ATOM
1604
N
LEU
A
107
58.363
−41.761
−4.446
1.00
13.69
N


ATOM
1605
CA
LEU
A
107
57.404
−42.146
−5.483
1.00
13.52
C


ATOM
1607
CB
LEU
A
107
57.124
−43.648
−5.421
1.00
13.66
C


ATOM
1610
CG
LEU
A
107
58.226
−44.573
−5.948
1.00
13.30
C


ATOM
1612
CD1
LEU
A
107
57.914
−46.015
−5.554
1.00
8.00
C


ATOM
1616
CD2
LEU
A
107
58.401
−44.452
−7.463
1.00
7.05
C


ATOM
1620
C
LEU
A
107
56.092
−41.356
−5.382
1.00
13.91
C


ATOM
1621
O
LEU
A
107
55.621
−40.801
−6.367
1.00
13.47
O


ATOM
1623
N
ARG
A
108
55.506
−41.288
−4.191
1.00
15.25
N


ATOM
1624
CA
ARG
A
108
54.240
−40.571
−4.036
1.00
15.54
C


ATOM
1626
CB
ARG
A
108
53.622
−40.792
−2.653
1.00
14.65
C


ATOM
1629
CG
ARG
A
108
52.283
−40.069
−2.498
1.00
14.11
C


ATOM
1632
CD
ARG
A
108
51.478
−40.584
−1.329
1.00
13.98
C


ATOM
1635
NE
ARG
A
108
50.905
−41.900
−1.590
1.00
13.21
N


ATOM
1637
CZ
ARG
A
108
50.220
−42.608
−0.699
1.00
11.52
C


ATOM
1638
NH1
ARG
A
108
50.016
−42.135
0.526
1.00
15.17
N


ATOM
1641
NH2
ARG
A
108
49.742
−43.797
−1.033
1.00
11.32
N


ATOM
1644
C
ARG
A
108
54.439
−39.083
−4.297
1.00
15.28
C


ATOM
1645
O
ARG
A
108
53.662
−38.455
−5.022
1.00
16.16
O


ATOM
1647
N
GLN
A
109
55.494
−38.527
−3.721
1.00
15.32
N


ATOM
1648
CA
GLN
A
109
55.827
−37.130
−3.931
1.00
16.35
C


ATOM
1650
CB
GLN
A
109
57.220
−36.830
−3.391
1.00
16.28
C


ATOM
1653
CG
GLN
A
109
57.749
−35.448
−3.742
1.00
16.44
C


ATOM
1656
CD
GLN
A
109
59.160
−35.218
−3.238
1.00
16.11
C


ATOM
1657
OE1
GLN
A
109
59.712
−36.020
−2.486
1.00
17.10
O


ATOM
1658
NE2
GLN
A
109
59.750
−34.121
−3.657
1.00
14.50
N


ATOM
1661
C
GLN
A
109
55.761
−36.775
−5.411
1.00
17.47
C


ATOM
1662
O
GLN
A
109
55.292
−35.703
−5.767
1.00
18.79
O


ATOM
1664
N
HIS
A
110
56.215
−37.694
−6.258
1.00
18.11
N


ATOM
1665
CA
HIS
A
110
56.276
−37.479
−7.692
1.00
17.45
C


ATOM
1667
CB
HIS
A
110
57.636
−37.946
−8.205
1.00
16.72
C


ATOM
1670
CG
HIS
A
110
58.751
−37.033
−7.802
1.00
18.12
C


ATOM
1671
ND1
HIS
A
110
59.086
−35.909
−8.526
1.00
20.00
N


ATOM
1673
CE1
HIS
A
110
60.076
−35.278
−7.918
1.00
19.23
C


ATOM
1675
NE2
HIS
A
110
60.387
−35.946
−6.822
1.00
14.94
N


ATOM
1677
CD2
HIS
A
110
59.567
−37.042
−6.720
1.00
16.29
C


ATOM
1679
C
HIS
A
110
55.113
−38.113
−8.465
1.00
18.62
C


ATOM
1680
O
HIS
A
110
55.185
−38.271
−9.688
1.00
19.67
O


ATOM
1682
N
GLY
A
111
54.035
−38.453
−7.760
1.00
18.20
N


ATOM
1683
CA
GLY
A
111
52.774
−38.816
−8.411
1.00
18.59
C


ATOM
1686
C
GLY
A
111
52.623
−40.265
−8.853
1.00
19.26
C


ATOM
1687
O
GLY
A
111
51.684
−40.595
−9.563
1.00
19.34
O


ATOM
1689
N
PHE
A
112
53.545
−41.130
−8.447
1.00
19.84
N


ATOM
1690
CA
PHE
A
112
53.432
−42.552
−8.726
1.00
20.51
C


ATOM
1692
CB
PHE
A
112
54.798
−43.231
−8.594
1.00
20.41
C


ATOM
1695
CG
PHE
A
112
55.781
−42.814
−9.652
1.00
22.40
C


ATOM
1696
CD1
PHE
A
112
55.858
−43.504
−10.853
1.00
24.53
C


ATOM
1698
CE1
PHE
A
112
56.754
−43.118
−11.842
1.00
25.91
C


ATOM
1700
CZ
PHE
A
112
57.589
−42.028
−11.639
1.00
25.44
C


ATOM
1702
CE2
PHE
A
112
57.526
−41.328
−10.448
1.00
24.79
C


ATOM
1704
CD2
PHE
A
112
56.620
−41.723
−9.458
1.00
26.40
C


ATOM
1706
C
PHE
A
112
52.432
−43.172
−7.762
1.00
21.15
C


ATOM
1707
O
PHE
A
112
52.259
−42.676
−6.646
1.00
22.39
O


ATOM
1709
N
GLU
A
113
51.760
−44.233
−8.204
1.00
21.63
N


ATOM
1710
CA
GLU
A
113
50.861
−45.009
−7.338
1.00
22.15
C


ATOM
1712
CB
GLU
A
113
49.839
−45.809
−8.152
1.00
23.20
C


ATOM
1715
CG
GLU
A
113
48.570
−45.030
−8.479
1.00
31.69
C


ATOM
1718
CD
GLU
A
113
47.538
−45.842
−9.263
1.00
38.97
C


ATOM
1719
OE1
GLU
A
113
47.666
−47.087
−9.346
1.00
36.67
O


ATOM
1720
OE2
GLU
A
113
46.586
−45.219
−9.788
1.00
44.13
O


ATOM
1721
C
GLU
A
113
51.647
−45.975
−6.474
1.00
19.83
C


ATOM
1722
O
GLU
A
113
52.300
−46.880
−6.996
1.00
19.13
O


ATOM
1724
N
VAL
A
114
51.582
−45.771
−5.159
1.00
17.92
N


ATOM
1725
CA
VAL
A
114
52.137
−46.708
−4.186
1.00
17.14
C


ATOM
1727
CB
VAL
A
114
53.416
−46.179
−3.524
1.00
16.87
C


ATOM
1729
CG1
VAL
A
114
54.578
−46.310
−4.477
1.00
18.62
C


ATOM
1733
CG2
VAL
A
114
53.242
−44.740
−3.074
1.00
13.97
C


ATOM
1737
C
VAL
A
114
51.101
−47.017
−3.115
1.00
17.20
C


ATOM
1738
O
VAL
A
114
50.317
−46.155
−2.729
1.00
16.91
O


ATOM
1740
N
SER
A
115
51.098
−48.261
−2.653
1.00
17.44
N


ATOM
1741
CA
SER
A
115
50.093
−48.744
−1.715
1.00
17.58
C


ATOM
1743
CB
SER
A
115
49.510
−50.067
−2.222
1.00
18.10
C


ATOM
1746
OG
SER
A
115
48.625
−50.653
−1.285
1.00
19.87
O


ATOM
1748
C
SER
A
115
50.749
−48.945
−0.367
1.00
17.16
C


ATOM
1749
O
SER
A
115
51.937
−49.249
−0.290
1.00
16.28
O


ATOM
1751
N
GLN
A
116
49.981
−48.782
0.702
1.00
17.89
N


ATOM
1752
CA
GLN
A
116
50.503
−49.064
2.037
1.00
18.00
C


ATOM
1754
CB
GLN
A
116
49.526
−48.604
3.124
1.00
17.32
C


ATOM
1757
CG
GLN
A
116
48.277
−49.444
3.250
1.00
18.24
C


ATOM
1760
CD
GLN
A
116
47.336
−48.939
4.323
1.00
18.00
C


ATOM
1761
OE1
GLN
A
116
47.647
−47.988
5.038
1.00
15.54
O


ATOM
1762
NE2
GLN
A
116
46.178
−49.585
4.448
1.00
15.61
N


ATOM
1765
C
GLN
A
116
50.858
−50.553
2.189
1.00
17.90
C


ATOM
1766
O
GLN
A
116
51.615
−50.913
3.085
1.00
16.82
O


ATOM
1768
N
GLU
A
117
50.332
−51.400
1.298
1.00
18.90
N


ATOM
1769
CA
GLU
A
117
50.718
−52.827
1.223
1.00
20.56
C


ATOM
1771
CB
GLU
A
117
49.960
−53.551
0.104
1.00
21.42
C


ATOM
1774
CG
GLU
A
117
48.453
−53.591
0.271
1.00
26.18
C


ATOM
1777
CD
GLU
A
117
47.764
−54.316
−0.870
1.00
32.39
C


ATOM
1778
OE1
GLU
A
117
47.922
−55.549
−0.962
1.00
37.41
O


ATOM
1779
OE2
GLU
A
117
47.058
−53.657
−1.667
1.00
37.11
O


ATOM
1780
C
GLU
A
117
52.218
−53.055
1.009
1.00
19.86
C


ATOM
1781
O
GLU
A
117
52.731
−54.133
1.304
1.00
20.64
O


ATOM
1783
N
ALA
A
118
52.914
−52.054
0.482
1.00
19.35
N


ATOM
1784
CA
ALA
A
118
54.369
−52.091
0.387
1.00
19.23
C


ATOM
1786
CB
ALA
A
118
54.896
−50.768
−0.178
1.00
18.70
C


ATOM
1790
C
ALA
A
118
55.043
−52.400
1.729
1.00
19.77
C


ATOM
1791
O
ALA
A
118
56.108
−53.011
1.758
1.00
21.53
O


ATOM
1793
N
PHE
A
119
54.427
−51.993
2.835
1.00
19.93
N


ATOM
1794
CA
PHE
A
119
55.022
−52.188
4.158
1.00
20.71
C


ATOM
1796
CB
PHE
A
119
54.604
−51.044
5.090
1.00
20.67
C


ATOM
1799
CG
PHE
A
119
55.135
−49.704
4.665
1.00
18.33
C


ATOM
1800
CD1
PHE
A
119
56.475
−49.395
4.834
1.00
16.96
C


ATOM
1802
CE1
PHE
A
119
56.983
−48.173
4.434
1.00
16.52
C


ATOM
1804
CZ
PHE
A
119
56.149
−47.239
3.859
1.00
20.21
C


ATOM
1806
CE2
PHE
A
119
54.797
−47.533
3.685
1.00
19.31
C


ATOM
1808
CD2
PHE
A
119
54.304
−48.765
4.082
1.00
19.09
C


ATOM
1810
C
PHE
A
119
54.704
−53.531
4.807
1.00
21.86
C


ATOM
1811
O
PHE
A
119
55.182
−53.810
5.906
1.00
22.54
O


ATOM
1813
N
SER
A
120
53.931
−54.375
4.127
1.00
23.53
N


ATOM
1814
CA
SER
A
120
53.434
−55.622
4.729
1.00
24.35
C


ATOM
1816
CB
SER
A
120
52.443
−56.329
3.798
1.00
24.27
C


ATOM
1819
OG
SER
A
120
53.064
−56.712
2.585
1.00
24.63
O


ATOM
1821
C
SER
A
120
54.532
−56.593
5.128
1.00
24.71
C


ATOM
1822
O
SER
A
120
54.361
−57.352
6.067
1.00
25.50
O


ATOM
1824
N
GLY
A
121
55.658
−56.568
4.425
1.00
25.99
N


ATOM
1825
CA
GLY
A
121
56.753
−57.494
4.710
1.00
26.71
C


ATOM
1828
C
GLY
A
121
57.490
−57.240
6.014
1.00
27.42
C


ATOM
1829
O
GLY
A
121
58.220
−58.105
6.487
1.00
28.57
O


ATOM
1831
N
PHE
A
122
57.304
−56.063
6.600
1.00
28.38
N


ATOM
1832
CA
PHE
A
122
58.040
−55.671
7.797
1.00
29.58
C


ATOM
1834
CB
PHE
A
122
58.606
−54.264
7.601
1.00
29.62
C


ATOM
1837
CG
PHE
A
122
59.415
−54.123
6.351
1.00
27.63
C


ATOM
1838
CD1
PHE
A
122
60.746
−54.496
6.330
1.00
26.30
C


ATOM
1840
CE1
PHE
A
122
61.494
−54.386
5.173
1.00
27.39
C


ATOM
1842
CZ
PHE
A
122
60.910
−53.905
4.016
1.00
27.09
C


ATOM
1844
CE2
PHE
A
122
59.579
−53.540
4.020
1.00
28.00
C


ATOM
1846
CD2
PHE
A
122
58.835
−53.652
5.184
1.00
28.29
C


ATOM
1848
C
PHE
A
122
57.197
−55.741
9.072
1.00
31.01
C


ATOM
1849
O
PHE
A
122
57.557
−55.149
10.095
1.00
30.74
O


ATOM
1851
N
LYS
A
123
56.087
−56.475
9.011
1.00
32.62
N


ATOM
1852
CA
LYS
A
123
55.214
−56.656
10.167
1.00
34.07
C


ATOM
1854
CB
LYS
A
123
53.767
−56.311
9.805
1.00
34.24
C


ATOM
1857
CG
LYS
A
123
53.525
−54.803
9.728
1.00
37.62
C


ATOM
1860
CD
LYS
A
123
52.080
−54.455
9.384
1.00
42.42
C


ATOM
1863
CE
LYS
A
123
51.801
−54.630
7.901
1.00
43.47
C


ATOM
1866
NZ
LYS
A
123
50.397
−54.325
7.535
1.00
44.54
N


ATOM
1870
C
LYS
A
123
55.339
−58.077
10.720
1.00
34.52
C


ATOM
1871
O
LYS
A
123
55.757
−58.998
10.014
1.00
34.29
O


ATOM
1873
N
ASP
A
124
54.990
−58.238
11.992
1.00
35.13
N


ATOM
1874
CA
ASP
A
124
55.210
−59.495
12.709
1.00
35.78
C


ATOM
1876
CB
ASP
A
124
55.701
−59.205
14.144
1.00
35.47
C


ATOM
1879
CG
ASP
A
124
54.628
−58.584
15.036
1.00
34.26
C


ATOM
1880
OD1
ASP
A
124
53.429
−58.695
14.720
1.00
33.09
O


ATOM
1881
OD2
ASP
A
124
54.988
−57.982
16.068
1.00
34.68
O


ATOM
1882
C
ASP
A
124
53.957
−60.386
12.710
1.00
36.80
C


ATOM
1883
O
ASP
A
124
53.014
−60.151
11.952
1.00
36.25
O


ATOM
1885
N
GLN
A
125
53.978
−61.414
13.559
1.00
37.90
N


ATOM
1886
CA
GLN
A
125
52.849
−62.326
13.772
1.00
38.52
C


ATOM
1888
CB
GLN
A
125
53.025
−63.037
15.120
1.00
38.94
C


ATOM
1891
CG
GLN
A
125
54.246
−63.958
15.208
1.00
41.34
C


ATOM
1894
CD
GLN
A
125
53.891
−65.410
15.483
1.00
43.70
C


ATOM
1895
OE1
GLN
A
125
52.855
−65.910
15.040
1.00
46.32
O


ATOM
1896
NE2
GLN
A
125
54.756
−66.096
16.223
1.00
43.96
N


ATOM
1899
C
GLN
A
125
51.491
−61.624
13.774
1.00
38.20
C


ATOM
1900
O
GLN
A
125
50.581
−62.010
13.036
1.00
38.02
O


ATOM
1902
N
ASN
A
126
51.375
−60.587
14.601
1.00
37.77
N


ATOM
1903
CA
ASN
A
126
50.088
−59.951
14.891
1.00
37.44
C


ATOM
1905
CB
ASN
A
126
49.985
−59.662
16.396
1.00
38.30
C


ATOM
1908
CG
ASN
A
126
50.409
−60.850
17.255
1.00
38.88
C


ATOM
1909
OD1
ASN
A
126
51.597
−61.043
17.524
1.00
38.90
O


ATOM
1910
ND2
ASN
A
126
49.438
−61.642
17.695
1.00
37.03
N


ATOM
1913
C
ASN
A
126
49.814
−58.664
14.100
1.00
36.17
C


ATOM
1914
O
ASN
A
126
48.929
−57.894
14.464
1.00
35.23
O


ATOM
1916
N
GLY
A
127
50.561
−58.439
13.022
1.00
35.58
N


ATOM
1917
CA
GLY
A
127
50.360
−57.266
12.171
1.00
35.27
C


ATOM
1920
C
GLY
A
127
50.951
−55.967
12.696
1.00
34.56
C


ATOM
1921
O
GLY
A
127
50.592
−54.891
12.220
1.00
34.71
O


ATOM
1923
N
ASN
A
128
51.848
−56.064
13.678
1.00
33.89
N


ATOM
1924
CA
ASN
A
128
52.581
−54.903
14.194
1.00
33.13
C


ATOM
1926
CB
ASN
A
128
52.707
−54.977
15.718
1.00
33.17
C


ATOM
1929
CG
ASN
A
128
51.360
−54.972
16.417
1.00
33.60
C


ATOM
1930
OD1
ASN
A
128
50.516
−54.113
16.161
1.00
33.68
O


ATOM
1931
ND2
ASN
A
128
51.153
−55.937
17.308
1.00
33.04
N


ATOM
1934
C
ASN
A
128
53.967
−54.854
13.569
1.00
31.75
C


ATOM
1935
O
ASN
A
128
54.506
−55.888
13.185
1.00
31.35
O


ATOM
1937
N
PHE
A
129
54.538
−53.657
13.462
1.00
30.95
N


ATOM
1938
CA
PHE
A
129
55.879
−53.497
12.889
1.00
30.05
C


ATOM
1940
CB
PHE
A
129
56.254
−52.014
12.763
1.00
29.58
C


ATOM
1943
CG
PHE
A
129
55.710
−51.358
11.528
1.00
26.87
C


ATOM
1944
CD1
PHE
A
129
56.350
−51.513
10.306
1.00
27.70
C


ATOM
1946
CE1
PHE
A
129
55.848
−50.912
9.155
1.00
26.40
C


ATOM
1948
CZ
PHE
A
129
54.697
−50.149
9.224
1.00
25.73
C


ATOM
1950
CE2
PHE
A
129
54.052
−49.986
10.439
1.00
25.95
C


ATOM
1952
CD2
PHE
A
129
54.559
−50.591
11.581
1.00
26.69
C


ATOM
1954
C
PHE
A
129
56.904
−54.236
13.744
1.00
29.69
C


ATOM
1955
O
PHE
A
129
56.896
−54.097
14.967
1.00
29.85
O


ATOM
1957
N
LEU
A
130
57.765
−55.022
13.094
1.00
29.51
N


ATOM
1958
CA
LEU
A
130
58.768
−55.851
13.781
1.00
30.01
C


ATOM
1960
CB
LEU
A
130
59.696
−56.532
12.771
1.00
29.98
C


ATOM
1963
CG
LEU
A
130
59.116
−57.641
11.888
1.00
31.00
C


ATOM
1965
CD1
LEU
A
130
59.911
−57.754
10.584
1.00
29.05
C


ATOM
1969
CD2
LEU
A
130
59.070
−58.985
12.628
1.00
29.48
C


ATOM
1973
C
LEU
A
130
59.615
−55.029
14.744
1.00
30.46
C


ATOM
1974
O
LEU
A
130
60.207
−54.031
14.354
1.00
29.85
O


ATOM
1976
N
GLU
A
131
59.678
−55.468
15.996
1.00
31.94
N


ATOM
1977
CA
GLU
A
131
60.332
−54.705
17.059
1.00
32.91
C


ATOM
1979
CB
GLU
A
131
60.176
−55.432
18.406
1.00
33.63
C


ATOM
1982
CG
GLU
A
131
60.572
−54.624
19.645
1.00
36.72
C


ATOM
1985
CD
GLU
A
131
59.574
−53.525
20.003
1.00
41.20
C


ATOM
1986
OE1
GLU
A
131
58.831
−53.043
19.116
1.00
42.35
O


ATOM
1987
OE2
GLU
A
131
59.542
−53.131
21.187
1.00
43.80
O


ATOM
1988
C
GLU
A
131
61.806
−54.439
16.755
1.00
32.75
C


ATOM
1989
O
GLU
A
131
62.278
−53.322
16.955
1.00
32.97
O


ATOM
1991
N
ASN
A
132
62.519
−55.447
16.247
1.00
32.39
N


ATOM
1992
CA
ASN
A
132
63.955
−55.305
15.963
1.00
32.47
C


ATOM
1994
CB
ASN
A
132
64.608
−56.663
15.690
1.00
32.78
C


ATOM
1997
CG
ASN
A
132
64.119
−57.311
14.409
1.00
34.78
C


ATOM
1998
OD1
ASN
A
132
64.090
−56.688
13.346
1.00
37.50
O


ATOM
1999
ND2
ASN
A
132
63.742
−58.581
14.502
1.00
37.80
N


ATOM
2002
C
ASN
A
132
64.293
−54.328
14.831
1.00
32.35
C


ATOM
2003
O
ASN
A
132
65.459
−53.979
14.649
1.00
32.24
O


ATOM
2005
N
LEU
A
133
63.288
−53.897
14.070
1.00
32.07
N


ATOM
2006
CA
LEU
A
133
63.482
−52.837
13.078
1.00
31.79
C


ATOM
2008
CB
LEU
A
133
62.191
−52.553
12.300
1.00
31.54
C


ATOM
2011
CG
LEU
A
133
61.723
−53.598
11.286
1.00
29.46
C


ATOM
2013
CD1
LEU
A
133
60.264
−53.365
10.920
1.00
27.57
C


ATOM
2017
CD2
LEU
A
133
62.596
−53.578
10.052
1.00
24.77
C


ATOM
2021
C
LEU
A
133
63.964
−51.543
13.730
1.00
32.07
C


ATOM
2022
O
LEU
A
133
64.597
−50.719
13.068
1.00
32.07
O


ATOM
2024
N
LYS
A
134
63.665
−51.373
15.020
1.00
32.16
N


ATOM
2025
CA
LYS
A
134
64.062
−50.178
15.767
1.00
32.51
C


ATOM
2027
CB
LYS
A
134
63.478
−50.215
17.186
1.00
32.54
C


ATOM
2030
CG
LYS
A
134
64.226
−51.130
18.165
1.00
36.12
C


ATOM
2033
CD
LYS
A
134
63.521
−51.195
19.521
1.00
36.37
C


ATOM
2036
CE
LYS
A
134
64.442
−51.707
20.620
1.00
36.60
C


ATOM
2039
NZ
LYS
A
134
63.891
−51.408
21.978
1.00
37.35
N


ATOM
2043
C
LYS
A
134
65.582
−49.978
15.831
1.00
32.46
C


ATOM
2044
O
LYS
A
134
66.048
−48.862
16.058
1.00
32.20
O


ATOM
2046
N
GLU
A
135
66.347
−51.055
15.642
1.00
32.82
N


ATOM
2047
CA
GLU
A
135
67.815
−50.977
15.655
1.00
33.11
C


ATOM
2049
CB
GLU
A
135
68.438
−52.380
15.759
1.00
33.80
C


ATOM
2052
CG
GLU
A
135
68.051
−53.174
17.014
1.00
37.61
C


ATOM
2055
CD
GLU
A
135
68.414
−52.472
18.322
1.00
43.02
C


ATOM
2056
OE1
GLU
A
135
69.243
−51.536
18.301
1.00
43.54
O


ATOM
2057
OE2
GLU
A
135
67.872
−52.864
19.381
1.00
46.53
O


ATOM
2058
C
GLU
A
135
68.406
−50.243
14.443
1.00
31.83
C


ATOM
2059
O
GLU
A
135
69.534
−49.752
14.512
1.00
31.59
O


ATOM
2061
N
ASP
A
136
67.650
−50.179
13.345
1.00
30.44
N


ATOM
2062
CA
ASP
A
136
68.060
−49.453
12.138
1.00
28.91
C


ATOM
2064
CB
ASP
A
136
67.644
−50.243
10.891
1.00
29.19
C


ATOM
2067
CG
ASP
A
136
68.128
−49.609
9.594
1.00
31.06
C


ATOM
2068
OD1
ASP
A
136
68.564
−48.439
9.606
1.00
35.55
O


ATOM
2069
OD2
ASP
A
136
68.064
−50.286
8.549
1.00
34.56
O


ATOM
2070
C
ASP
A
136
67.430
−48.058
12.141
1.00
27.38
C


ATOM
2071
O
ASP
A
136
66.292
−47.884
11.722
1.00
26.60
O


ATOM
2073
N
ILE
A
137
68.187
−47.064
12.598
1.00
26.48
N


ATOM
2074
CA
ILE
A
137
67.656
−45.713
12.803
1.00
26.13
C


ATOM
2076
CB
ILE
A
137
68.610
−44.865
13.661
1.00
25.62
C


ATOM
2078
CG1
ILE
A
137
68.839
−45.537
15.020
1.00
28.03
C


ATOM
2081
CD1
ILE
A
137
67.571
−46.035
15.700
1.00
28.59
C


ATOM
2085
CG2
ILE
A
137
68.064
−43.461
13.854
1.00
22.91
C


ATOM
2089
C
ILE
A
137
67.358
−44.977
11.496
1.00
27.13
C


ATOM
2090
O
ILE
A
137
66.354
−44.265
11.399
1.00
28.15
O


ATOM
2092
N
LYS
A
138
68.230
−45.136
10.502
1.00
27.01
N


ATOM
2093
CA
LYS
A
138
67.969
−44.623
9.156
1.00
26.64
C


ATOM
2095
CB
LYS
A
138
68.993
−45.178
8.157
1.00
28.39
C


ATOM
2098
CG
LYS
A
138
70.151
−44.245
7.807
1.00
32.14
C


ATOM
2101
CD
LYS
A
138
71.022
−44.874
6.709
1.00
38.28
C


ATOM
2104
CE
LYS
A
138
71.858
−43.837
5.972
1.00
41.36
C


ATOM
2107
NZ
LYS
A
138
72.680
−44.443
4.885
1.00
40.92
N


ATOM
2111
C
LYS
A
138
66.567
−45.009
8.681
1.00
25.05
C


ATOM
2112
O
LYS
A
138
65.827
−44.170
8.150
1.00
24.81
O


ATOM
2114
N
ALA
A
139
66.221
−46.282
8.876
1.00
22.85
N


ATOM
2115
CA
ALA
A
139
64.957
−46.835
8.405
1.00
21.98
C


ATOM
2117
CB
ALA
A
139
64.986
−48.349
8.474
1.00
22.02
C


ATOM
2121
C
ALA
A
139
63.777
−46.294
9.202
1.00
21.49
C


ATOM
2122
O
ALA
A
139
62.753
−45.917
8.633
1.00
21.39
O


ATOM
2124
N
ILE
A
140
63.922
−46.252
10.518
1.00
20.92
N


ATOM
2125
CA
ILE
A
140
62.882
−45.694
11.366
1.00
20.56
C


ATOM
2127
CB
ILE
A
140
63.245
−45.810
12.859
1.00
20.40
C


ATOM
2129
CG1
ILE
A
140
63.319
−47.281
13.278
1.00
21.59
C


ATOM
2132
CD1
ILE
A
140
62.031
−48.075
13.047
1.00
18.82
C


ATOM
2136
CG2
ILE
A
140
62.223
−45.099
13.720
1.00
20.44
C


ATOM
2140
C
ILE
A
140
62.640
−44.234
10.981
1.00
20.43
C


ATOM
2141
O
ILE
A
140
61.495
−43.788
10.915
1.00
21.00
O


ATOM
2143
N
LEU
A
141
63.716
−43.500
10.707
1.00
19.96
N


ATOM
2144
CA
LEU
A
141
63.598
−42.114
10.239
1.00
19.90
C


ATOM
2146
CB
LEU
A
141
64.968
−41.432
10.124
1.00
20.05
C


ATOM
2149
CG
LEU
A
141
65.429
−40.669
11.359
1.00
21.37
C


ATOM
2151
CD1
LEU
A
141
66.855
−40.215
11.165
1.00
19.36
C


ATOM
2155
CD2
LEU
A
141
64.495
−39.480
11.627
1.00
20.19
C


ATOM
2159
C
LEU
A
141
62.894
−42.035
8.901
1.00
18.88
C


ATOM
2160
O
LEU
A
141
62.067
−41.161
8.706
1.00
18.87
O


ATOM
2162
N
SER
A
142
63.233
−42.938
7.982
1.00
18.38
N


ATOM
2163
CA
SER
A
142
62.579
−42.963
6.677
1.00
18.87
C


ATOM
2165
CB
SER
A
142
63.227
−43.992
5.755
1.00
18.85
C


ATOM
2168
OG
SER
A
142
64.441
−43.499
5.224
1.00
24.59
O


ATOM
2170
C
SER
A
142
61.093
−43.262
6.819
1.00
18.39
C


ATOM
2171
O
SER
A
142
60.257
−42.650
6.152
1.00
18.06
O


ATOM
2173
N
LEU
A
143
60.772
−44.206
7.696
1.00
17.92
N


ATOM
2174
CA
LEU
A
143
59.392
−44.581
7.928
1.00
17.92
C


ATOM
2176
CB
LEU
A
143
59.312
−45.750
8.905
1.00
17.59
C


ATOM
2179
CG
LEU
A
143
57.928
−46.353
9.099
1.00
18.56
C


ATOM
2181
CD1
LEU
A
143
57.368
−46.849
7.762
1.00
16.28
C


ATOM
2185
CD2
LEU
A
143
57.994
−47.480
10.140
1.00
12.92
C


ATOM
2189
C
LEU
A
143
58.625
−43.379
8.464
1.00
17.94
C


ATOM
2190
O
LEU
A
143
57.545
−43.056
7.958
1.00
19.38
O


ATOM
2192
N
TYR
A
144
59.196
−42.710
9.464
1.00
16.49
N


ATOM
2193
CA
TYR
A
144
58.591
−41.509
10.038
1.00
16.63
C


ATOM
2195
CB
TYR
A
144
59.527
−40.881
11.091
1.00
16.88
C


ATOM
2198
CG
TYR
A
144
59.109
−39.513
11.614
1.00
16.47
C


ATOM
2199
CD1
TYR
A
144
58.142
−39.376
12.617
1.00
17.87
C


ATOM
2201
CE1
TYR
A
144
57.773
−38.107
13.102
1.00
17.50
C


ATOM
2203
CZ
TYR
A
144
58.380
−36.971
12.569
1.00
20.12
C


ATOM
2204
OH
TYR
A
144
58.040
−35.708
13.007
1.00
23.04
O


ATOM
2206
CE2
TYR
A
144
59.335
−37.092
11.575
1.00
17.73
C


ATOM
2208
CD2
TYR
A
144
59.694
−38.354
11.108
1.00
20.73
C


ATOM
2210
C
TYR
A
144
58.249
−40.500
8.949
1.00
16.24
C


ATOM
2211
O
TYR
A
144
57.127
−39.998
8.904
1.00
16.38
O


ATOM
2213
N
GLU
A
145
59.206
−40.240
8.057
1.00
16.21
N


ATOM
2214
CA
GLU
A
145
59.044
−39.217
7.023
1.00
16.50
C


ATOM
2216
CB
GLU
A
145
60.362
−38.931
6.309
1.00
16.55
C


ATOM
2219
CG
GLU
A
145
61.487
−38.436
7.214
1.00
19.48
C


ATOM
2222
CD
GLU
A
145
61.341
−36.995
7.676
1.00
24.18
C


ATOM
2223
OE1
GLU
A
145
60.328
−36.326
7.360
1.00
25.12
O


ATOM
2224
OE2
GLU
A
145
62.272
−36.534
8.374
1.00
30.41
O


ATOM
2225
C
GLU
A
145
57.995
−39.615
6.002
1.00
16.20
C


ATOM
2226
O
GLU
A
145
57.247
−38.768
5.523
1.00
17.56
O


ATOM
2228
N
ALA
A
146
57.928
−40.902
5.683
1.00
15.45
N


ATOM
2229
CA
ALA
A
146
56.947
−41.396
4.725
1.00
14.88
C


ATOM
2231
CB
ALA
A
146
57.236
−42.843
4.359
1.00
14.69
C


ATOM
2235
C
ALA
A
146
55.540
−41.266
5.277
1.00
14.01
C


ATOM
2236
O
ALA
A
146
54.617
−40.964
4.540
1.00
13.57
O


ATOM
2238
N
SER
A
147
55.385
−41.474
6.579
1.00
14.63
N


ATOM
2239
CA
SER
A
147
54.061
−41.533
7.197
1.00
15.33
C


ATOM
2241
CB
SER
A
147
54.185
−41.828
8.695
1.00
14.91
C


ATOM
2244
OG
SER
A
147
54.504
−40.663
9.423
1.00
16.45
O


ATOM
2246
C
SER
A
147
53.212
−40.269
6.988
1.00
16.17
C


ATOM
2247
O
SER
A
147
51.985
−40.346
6.986
1.00
16.00
O


ATOM
2249
N
PHE
A
148
53.864
−39.118
6.820
1.00
16.36
N


ATOM
2250
CA
PHE
A
148
53.157
−37.850
6.658
1.00
15.19
C


ATOM
2252
CB
PHE
A
148
54.055
−36.678
7.067
1.00
15.31
C


ATOM
2255
CG
PHE
A
148
54.380
−36.649
8.531
1.00
12.93
C


ATOM
2256
CD1
PHE
A
148
55.501
−37.310
9.016
1.00
15.72
C


ATOM
2258
CE1
PHE
A
148
55.817
−37.301
10.371
1.00
11.87
C


ATOM
2260
CZ
PHE
A
148
55.001
−36.628
11.261
1.00
12.42
C


ATOM
2262
CE2
PHE
A
148
53.871
−35.957
10.787
1.00
15.49
C


ATOM
2264
CD2
PHE
A
148
53.572
−35.970
9.424
1.00
10.40
C


ATOM
2266
C
PHE
A
148
52.621
−37.627
5.242
1.00
16.28
C


ATOM
2267
O
PHE
A
148
51.908
−36.653
4.998
1.00
17.46
O


ATOM
2269
N
LEU
A
149
52.952
−38.516
4.308
1.00
16.51
N


ATOM
2270
CA
LEU
A
149
52.374
−38.463
2.963
1.00
16.07
C


ATOM
2272
CB
LEU
A
149
53.410
−38.866
1.911
1.00
15.09
C


ATOM
2275
CG
LEU
A
149
54.466
−37.796
1.583
1.00
17.25
C


ATOM
2277
CD1
LEU
A
149
55.499
−37.629
2.714
1.00
8.39
C


ATOM
2281
CD2
LEU
A
149
55.169
−38.124
0.265
1.00
15.29
C


ATOM
2285
C
LEU
A
149
51.123
−39.343
2.865
1.00
17.09
C


ATOM
2286
O
LEU
A
149
50.629
−39.619
1.769
1.00
18.27
O


ATOM
2288
N
ALA
A
150
50.600
−39.758
4.015
1.00
16.86
N


ATOM
2289
CA
ALA
A
150
49.436
−40.618
4.070
1.00
17.38
C


ATOM
2291
CB
ALA
A
150
49.184
−41.071
5.499
1.00
16.69
C


ATOM
2295
C
ALA
A
150
48.191
−39.934
3.523
1.00
18.43
C


ATOM
2296
O
ALA
A
150
48.045
−38.715
3.610
1.00
17.31
O


ATOM
2298
N
LEU
A
151
47.302
−40.748
2.958
1.00
19.16
N


ATOM
2299
CA
LEU
A
151
45.975
−40.315
2.560
1.00
18.86
C


ATOM
2301
CB
LEU
A
151
45.615
−40.901
1.194
1.00
19.43
C


ATOM
2304
CG
LEU
A
151
46.562
−40.592
0.027
1.00
20.03
C


ATOM
2306
CD1
LEU
A
151
45.910
−41.014
−1.287
1.00
17.51
C


ATOM
2310
CD2
LEU
A
151
46.938
−39.122
−0.020
1.00
12.04
C


ATOM
2314
C
LEU
A
151
44.996
−40.820
3.604
1.00
18.10
C


ATOM
2315
O
LEU
A
151
45.347
−41.693
4.399
1.00
18.33
O


ATOM
2317
N
GLU
A
152
43.776
−40.277
3.601
1.00
16.98
N


ATOM
2318
CA
GLU
A
152
42.715
−40.777
4.468
1.00
16.71
C


ATOM
2320
CB
GLU
A
152
41.402
−40.056
4.215
1.00
16.62
C


ATOM
2323
CG
GLU
A
152
41.343
−38.679
4.839
1.00
20.72
C


ATOM
2326
CD
GLU
A
152
39.957
−38.085
4.813
1.00
21.47
C


ATOM
2327
OE1
GLU
A
152
39.588
−37.500
3.770
1.00
21.16
O


ATOM
2328
OE2
GLU
A
152
39.255
−38.197
5.842
1.00
20.77
O


ATOM
2329
C
GLU
A
152
42.513
−42.264
4.256
1.00
16.79
C


ATOM
2330
O
GLU
A
152
42.488
−42.743
3.120
1.00
17.14
O


ATOM
2332
N
GLY
A
153
42.398
−42.992
5.361
1.00
17.10
N


ATOM
2333
CA
GLY
A
153
42.193
−44.434
5.321
1.00
16.68
C


ATOM
2336
C
GLY
A
153
43.449
−45.280
5.205
1.00
15.90
C


ATOM
2337
O
GLY
A
153
43.356
−46.508
5.215
1.00
15.88
O


ATOM
2339
N
GLU
A
154
44.620
−44.653
5.089
1.00
14.94
N


ATOM
2340
CA
GLU
A
154
45.869
−45.414
5.090
1.00
15.59
C


ATOM
2342
CB
GLU
A
154
46.934
−44.738
4.228
1.00
15.38
C


ATOM
2345
CG
GLU
A
154
46.607
−44.803
2.747
1.00
15.65
C


ATOM
2348
CD
GLU
A
154
47.740
−44.311
1.864
1.00
18.08
C


ATOM
2349
OE1
GLU
A
154
48.355
−43.266
2.194
1.00
13.19
O


ATOM
2350
OE2
GLU
A
154
48.005
−44.966
0.825
1.00
13.56
O


ATOM
2351
C
GLU
A
154
46.352
−45.637
6.529
1.00
15.87
C


ATOM
2352
O
GLU
A
154
47.347
−45.070
6.969
1.00
16.19
O


ATOM
2354
N
ASN
A
155
45.628
−46.493
7.240
1.00
15.55
N


ATOM
2355
CA
ASN
A
155
45.861
−46.753
8.653
1.00
15.76
C


ATOM
2357
CB
ASN
A
155
44.825
−47.762
9.180
1.00
16.25
C


ATOM
2360
CG
ASN
A
155
44.853
−49.080
8.423
1.00
14.85
C


ATOM
2361
OD1
ASN
A
155
44.665
−49.116
7.212
1.00
13.33
O


ATOM
2362
ND2
ASN
A
155
45.097
−50.160
9.134
1.00
12.78
N


ATOM
2365
C
ASN
A
155
47.263
−47.260
8.984
1.00
15.84
C


ATOM
2366
O
ASN
A
155
47.732
−47.076
10.108
1.00
14.90
O


ATOM
2368
N
ILE
A
156
47.923
−47.907
8.021
1.00
15.44
N


ATOM
2369
CA
ILE
A
156
49.255
−48.446
8.254
1.00
15.42
C


ATOM
2371
CB
ILE
A
156
49.616
−49.526
7.220
1.00
15.75
C


ATOM
2373
CG1
ILE
A
156
48.749
−50.766
7.457
1.00
17.79
C


ATOM
2376
CD1
ILE
A
156
49.062
−51.899
6.535
1.00
23.00
C


ATOM
2380
CG2
ILE
A
156
51.078
−49.917
7.326
1.00
14.73
C


ATOM
2384
C
ILE
A
156
50.304
−47.337
8.318
1.00
16.34
C


ATOM
2385
O
ILE
A
156
51.244
−47.419
9.103
1.00
17.37
O


ATOM
2387
N
LEU
A
157
50.140
−46.292
7.517
1.00
16.79
N


ATOM
2388
CA
LEU
A
157
51.022
−45.137
7.616
1.00
17.34
C


ATOM
2390
CB
LEU
A
157
50.823
−44.186
6.437
1.00
17.21
C


ATOM
2393
CG
LEU
A
157
51.173
−44.788
5.072
1.00
15.12
C


ATOM
2395
CD1
LEU
A
157
50.923
−43.812
3.947
1.00
12.69
C


ATOM
2399
CD2
LEU
A
157
52.594
−45.249
5.047
1.00
15.60
C


ATOM
2403
C
LEU
A
157
50.800
−44.428
8.952
1.00
18.32
C


ATOM
2404
O
LEU
A
157
51.751
−43.997
9.588
1.00
18.18
O


ATOM
2406
N
ASP
A
158
49.551
−44.345
9.400
1.00
19.27
N


ATOM
2407
CA
ASP
A
158
49.258
−43.784
10.721
1.00
19.57
C


ATOM
2409
CB
ASP
A
158
47.747
−43.677
10.960
1.00
18.96
C


ATOM
2412
CG
ASP
A
158
47.112
−42.492
10.224
1.00
22.24
C


ATOM
2413
OD1
ASP
A
158
47.680
−42.013
9.216
1.00
23.78
O


ATOM
2414
OD2
ASP
A
158
46.025
−42.048
10.651
1.00
22.36
O


ATOM
2415
C
ASP
A
158
49.915
−44.602
11.830
1.00
19.81
C


ATOM
2416
O
ASP
A
158
50.439
−44.038
12.788
1.00
19.90
O


ATOM
2418
N
GLU
A
159
49.887
−45.926
11.688
1.00
20.82
N


ATOM
2419
CA
GLU
A
159
50.496
−46.838
12.660
1.00
20.62
C


ATOM
2421
CB
GLU
A
159
50.021
−48.274
12.415
1.00
21.05
C


ATOM
2424
CG
GLU
A
159
48.552
−48.504
12.764
1.00
26.10
C


ATOM
2427
CD
GLU
A
159
48.001
−49.837
12.250
1.00
31.55
C


ATOM
2428
OE1
GLU
A
159
48.803
−50.695
11.814
1.00
33.98
O


ATOM
2429
OE2
GLU
A
159
46.762
−50.025
12.298
1.00
30.97
O


ATOM
2430
C
GLU
A
159
52.019
−46.767
12.585
1.00
19.71
C


ATOM
2431
O
GLU
A
159
52.703
−46.879
13.596
1.00
19.92
O


ATOM
2433
N
ALA
A
160
52.540
−46.593
11.376
1.00
19.00
N


ATOM
2434
CA
ALA
A
160
53.970
−46.408
11.169
1.00
18.47
C


ATOM
2436
CB
ALA
A
160
54.285
−46.338
9.675
1.00
16.51
C


ATOM
2440
C
ALA
A
160
54.465
−45.149
11.889
1.00
18.12
C


ATOM
2441
O
ALA
A
160
55.519
−45.161
12.512
1.00
17.45
O


ATOM
2443
N
LYS
A
161
53.695
−44.070
11.807
1.00
18.62
N


ATOM
2444
CA
LYS
A
161
54.059
−42.836
12.474
1.00
20.31
C


ATOM
2446
CB
LYS
A
161
53.020
−41.741
12.229
1.00
20.68
C


ATOM
2449
CG
LYS
A
161
53.518
−40.345
12.595
1.00
23.13
C


ATOM
2452
CD
LYS
A
161
52.384
−39.357
12.792
1.00
25.25
C


ATOM
2455
CE
LYS
A
161
51.729
−38.985
11.488
1.00
25.89
C


ATOM
2458
NZ
LYS
A
161
50.523
−38.158
11.743
1.00
29.77
N


ATOM
2462
C
LYS
A
161
54.215
−43.076
13.972
1.00
21.54
C


ATOM
2463
O
LYS
A
161
55.273
−42.812
14.533
1.00
23.15
O


ATOM
2465
N
VAL
A
162
53.171
−43.600
14.608
1.00
21.22
N


ATOM
2466
CA
VAL
A
162
53.181
−43.828
16.051
1.00
20.51
C


ATOM
2468
CB
VAL
A
162
51.830
−44.437
16.533
1.00
20.85
C


ATOM
2470
CG1
VAL
A
162
51.887
−44.807
18.010
1.00
20.30
C


ATOM
2474
CG2
VAL
A
162
50.680
−43.468
16.279
1.00
16.19
C


ATOM
2478
C
VAL
A
162
54.360
−44.719
16.473
1.00
20.71
C


ATOM
2479
O
VAL
A
162
54.976
−44.491
17.514
1.00
21.67
O


ATOM
2481
N
PHE
A
163
54.669
−45.717
15.651
1.00
20.50
N


ATOM
2482
CA
PHE
A
163
55.743
−46.678
15.924
1.00
20.68
C


ATOM
2484
CB
PHE
A
163
55.613
−47.861
14.949
1.00
20.40
C


ATOM
2487
CG
PHE
A
163
56.771
−48.828
14.960
1.00
18.51
C


ATOM
2488
CD1
PHE
A
163
56.819
−49.868
15.876
1.00
17.62
C


ATOM
2490
CE1
PHE
A
163
57.870
−50.785
15.866
1.00
16.93
C


ATOM
2492
CZ
PHE
A
163
58.884
−50.675
14.920
1.00
15.97
C


ATOM
2494
CE2
PHE
A
163
58.837
−49.648
13.985
1.00
18.38
C


ATOM
2496
CD2
PHE
A
163
57.779
−48.737
14.005
1.00
18.12
C


ATOM
2498
C
PHE
A
163
57.106
−45.998
15.803
1.00
21.49
C


ATOM
2499
O
PHE
A
163
57.936
−46.086
16.705
1.00
21.23
O


ATOM
2501
N
ALA
A
164
57.323
−45.311
14.688
1.00
22.41
N


ATOM
2502
CA
ALA
A
164
58.558
−44.564
14.473
1.00
23.01
C


ATOM
2504
CB
ALA
A
164
58.499
−43.802
13.149
1.00
22.58
C


ATOM
2508
C
ALA
A
164
58.819
−43.595
15.625
1.00
23.79
C


ATOM
2509
O
ALA
A
164
59.877
−43.646
16.237
1.00
24.21
O


ATOM
2511
N
ILE
A
165
57.848
−42.732
15.917
1.00
24.41
N


ATOM
2512
CA
ILE
A
165
57.994
−41.703
16.943
1.00
25.72
C


ATOM
2514
CB
ILE
A
165
56.692
−40.881
17.129
1.00
25.90
C


ATOM
2516
CG1
ILE
A
165
56.446
−39.976
15.921
1.00
27.50
C


ATOM
2519
CD1
ILE
A
165
55.312
−38.984
16.110
1.00
29.50
C


ATOM
2523
CG2
ILE
A
165
56.776
−40.020
18.377
1.00
26.20
C


ATOM
2527
C
ILE
A
165
58.396
−42.307
18.288
1.00
27.31
C


ATOM
2528
O
ILE
A
165
59.373
−41.880
18.900
1.00
27.00
O


ATOM
2530
N
SER
A
166
57.653
−43.315
18.734
1.00
28.52
N


ATOM
2531
CA
SER
A
166
57.888
−43.906
20.043
1.00
28.85
C


ATOM
2533
CB
SER
A
166
56.927
−45.074
20.306
1.00
28.71
C


ATOM
2536
OG
SER
A
166
57.305
−46.238
19.588
1.00
29.11
O


ATOM
2538
C
SER
A
166
59.343
−44.355
20.185
1.00
29.63
C


ATOM
2539
O
SER
A
166
59.927
−44.231
21.259
1.00
30.04
O


ATOM
2541
N
HIS
A
167
59.925
−44.855
19.098
1.00
30.55
N


ATOM
2542
CA
HIS
A
167
61.303
−45.346
19.119
1.00
32.12
C


ATOM
2544
CB
HIS
A
167
61.479
−46.544
18.178
1.00
32.45
C


ATOM
2547
CG
HIS
A
167
60.919
−47.827
18.725
1.00
39.05
C


ATOM
2548
ND1
HIS
A
167
60.459
−48.846
17.917
1.00
42.96
N


ATOM
2550
CE1
HIS
A
167
60.029
−49.844
18.671
1.00
43.16
C


ATOM
2552
NE2
HIS
A
167
60.184
−49.507
19.939
1.00
42.42
N


ATOM
2554
CD2
HIS
A
167
60.739
−48.250
20.001
1.00
42.90
C


ATOM
2556
C
HIS
A
167
62.344
−44.269
18.826
1.00
32.14
C


ATOM
2557
O
HIS
A
167
63.475
−44.383
19.285
1.00
33.11
O


ATOM
2559
N
LEU
A
168
61.971
−43.230
18.084
1.00
32.08
N


ATOM
2560
CA
LEU
A
168
62.864
−42.087
17.862
1.00
32.28
C


ATOM
2562
CB
LEU
A
168
62.381
−41.237
16.678
1.00
31.81
C


ATOM
2565
CG
LEU
A
168
62.424
−41.885
15.288
1.00
29.10
C


ATOM
2567
CD1
LEU
A
168
61.731
−40.998
14.274
1.00
21.41
C


ATOM
2571
CD2
LEU
A
168
63.849
−42.204
14.849
1.00
23.48
C


ATOM
2575
C
LEU
A
168
62.981
−41.206
19.111
1.00
33.66
C


ATOM
2576
O
LEU
A
168
64.044
−40.658
19.397
1.00
33.20
O


ATOM
2578
N
LYS
A
169
61.881
−41.086
19.848
1.00
36.11
N


ATOM
2579
CA
LYS
A
169
61.786
−40.181
20.998
1.00
37.70
C


ATOM
2581
CB
LYS
A
169
60.315
−40.050
21.421
1.00
37.80
C


ATOM
2584
CG
LYS
A
169
59.992
−38.859
22.309
1.00
41.71
C


ATOM
2587
CD
LYS
A
169
58.478
−38.569
22.345
1.00
44.58
C


ATOM
2590
CE
LYS
A
169
58.036
−37.704
21.160
1.00
45.75
C


ATOM
2593
NZ
LYS
A
169
56.591
−37.345
21.192
1.00
43.84
N


ATOM
2597
C
LYS
A
169
62.659
−40.642
22.176
1.00
38.59
C


ATOM
2598
O
LYS
A
169
63.166
−39.817
22.939
1.00
37.70
O


ATOM
2600
N
GLU
A
170
62.849
−41.954
22.304
1.00
40.22
N


ATOM
2601
CA
GLU
A
170
63.670
−42.523
23.380
1.00
41.64
C


ATOM
2603
CB
GLU
A
170
62.947
−43.714
24.032
1.00
41.66
C


ATOM
2606
CG
GLU
A
170
61.570
−43.379
24.620
1.00
43.09
C


ATOM
2609
CD
GLU
A
170
61.615
−42.245
25.636
1.00
46.14
C


ATOM
2610
OE1
GLU
A
170
62.239
−42.418
26.707
1.00
48.22
O


ATOM
2611
OE2
GLU
A
170
61.023
−41.178
25.363
1.00
47.78
O


ATOM
2612
C
GLU
A
170
65.063
−42.941
22.882
1.00
42.52
C


ATOM
2613
O
GLU
A
170
65.528
−44.050
23.159
1.00
41.63
O


ATOM
2615
N
LEU
A
171
65.719
−42.038
22.152
1.00
43.75
N


ATOM
2616
CA
LEU
A
171
67.086
−42.247
21.675
1.00
44.67
C


ATOM
2618
CB
LEU
A
171
67.165
−42.090
20.152
1.00
44.05
C


ATOM
2621
CG
LEU
A
171
66.660
−43.241
19.282
1.00
43.10
C


ATOM
2623
CD1
LEU
A
171
66.666
−42.836
17.816
1.00
42.94
C


ATOM
2627
CD2
LEU
A
171
67.487
−44.495
19.481
1.00
41.69
C


ATOM
2631
C
LEU
A
171
68.026
−41.239
22.323
1.00
45.97
C


ATOM
2632
O
LEU
A
171
67.711
−40.048
22.400
1.00
45.99
O


ATOM
2634
N
SER
A
172
69.183
−41.722
22.776
1.00
47.57
N


ATOM
2635
CA
SER
A
172
70.232
−40.854
23.314
1.00
48.79
C


ATOM
2637
CB
SER
A
172
70.887
−41.476
24.554
1.00
49.01
C


ATOM
2640
OG
SER
A
172
70.147
−41.178
25.726
1.00
50.28
O


ATOM
2642
C
SER
A
172
71.296
−40.564
22.256
1.00
49.34
C


ATOM
2643
O
SER
A
172
71.616
−41.417
21.416
1.00
49.13
O


ATOM
2645
N
GLU
A
173
71.838
−39.350
22.320
1.00
49.90
N


ATOM
2646
CA
GLU
A
173
72.919
−38.900
21.441
1.00
50.29
C


ATOM
2648
CB
GLU
A
173
73.304
−37.459
21.819
1.00
50.50
C


ATOM
2651
CG
GLU
A
173
74.414
−36.821
20.988
1.00
51.47
C


ATOM
2654
CD
GLU
A
173
74.555
−35.329
21.257
1.00
53.10
C


ATOM
2655
OE1
GLU
A
173
73.522
−34.662
21.480
1.00
53.40
O


ATOM
2656
OE2
GLU
A
173
75.695
−34.818
21.241
1.00
55.08
O


ATOM
2657
C
GLU
A
173
74.139
−39.826
21.519
1.00
49.97
C


ATOM
2658
O
GLU
A
173
74.787
−40.100
20.504
1.00
49.13
O


ATOM
2660
N
GLU
A
174
74.419
−40.323
22.724
1.00
50.10
N


ATOM
2661
CA
GLU
A
174
75.610
−41.127
22.990
1.00
50.67
C


ATOM
2663
CB
GLU
A
174
75.826
−41.290
24.502
1.00
51.28
C


ATOM
2666
CG
GLU
A
174
76.235
−39.999
25.224
1.00
53.48
C


ATOM
2669
CD
GLU
A
174
75.055
−39.119
25.632
1.00
57.27
C


ATOM
2670
OE1
GLU
A
174
73.975
−39.212
25.001
1.00
57.63
O


ATOM
2671
OE2
GLU
A
174
75.214
−38.324
26.587
1.00
57.70
O


ATOM
2672
C
GLU
A
174
75.566
−42.500
22.321
1.00
50.40
C


ATOM
2673
O
GLU
A
174
76.612
−43.076
22.017
1.00
50.44
O


ATOM
2675
N
LYS
A
175
74.363
−43.015
22.082
1.00
50.05
N


ATOM
2676
CA
LYS
A
175
74.192
−44.347
21.492
1.00
49.51
C


ATOM
2678
CB
LYS
A
175
72.820
−44.928
21.859
1.00
50.11
C


ATOM
2681
CG
LYS
A
175
72.507
−44.943
23.356
1.00
52.68
C


ATOM
2684
CD
LYS
A
175
71.119
−45.522
23.632
1.00
54.40
C


ATOM
2687
CE
LYS
A
175
70.933
−45.874
25.106
1.00
54.94
C


ATOM
2690
NZ
LYS
A
175
71.780
−47.027
25.528
1.00
54.51
N


ATOM
2694
C
LYS
A
175
74.333
−44.335
19.970
1.00
48.28
C


ATOM
2695
O
LYS
A
175
74.935
−45.242
19.399
1.00
47.51
O


ATOM
2697
N
ILE
A
176
73.775
−43.311
19.322
1.00
47.24
N


ATOM
2698
CA
ILE
A
176
73.623
−43.301
17.856
1.00
46.13
C


ATOM
2700
CB
ILE
A
176
72.214
−42.811
17.439
1.00
45.78
C


ATOM
2702
CG1
ILE
A
176
72.000
−41.327
17.788
1.00
44.12
C


ATOM
2705
CD1
ILE
A
176
70.595
−40.830
17.518
1.00
39.62
C


ATOM
2709
CG2
ILE
A
176
71.154
−43.685
18.086
1.00
45.67
C


ATOM
2713
C
ILE
A
176
74.669
−42.486
17.097
1.00
45.92
C


ATOM
2714
O
ILE
A
176
74.983
−42.799
15.945
1.00
45.28
O


ATOM
2716
N
GLY
A
177
75.186
−41.440
17.739
1.00
45.76
N


ATOM
2717
CA
GLY
A
177
76.150
−40.531
17.122
1.00
45.48
C


ATOM
2720
C
GLY
A
177
75.651
−39.107
17.209
1.00
45.05
C


ATOM
2721
O
GLY
A
177
74.472
−38.879
17.464
1.00
45.46
O


ATOM
2723
N
LYS
A
178
76.548
−38.149
17.001
1.00
44.56
N


ATOM
2724
CA
LYS
A
178
76.185
−36.731
17.034
1.00
44.02
C


ATOM
2726
CB
LYS
A
178
77.446
−35.850
17.054
1.00
44.19
C


ATOM
2729
CG
LYS
A
178
77.210
−34.339
16.906
1.00
46.02
C


ATOM
2732
CD
LYS
A
178
76.446
−33.747
18.081
1.00
48.86
C


ATOM
2735
CE
LYS
A
178
76.178
−32.256
17.883
1.00
49.84
C


ATOM
2738
NZ
LYS
A
178
77.393
−31.420
18.104
1.00
49.97
N


ATOM
2742
C
LYS
A
178
75.290
−36.365
15.846
1.00
43.03
C


ATOM
2743
O
LYS
A
178
74.349
−35.583
15.997
1.00
42.69
O


ATOM
2745
N
GLU
A
179
75.572
−36.937
14.677
1.00
41.61
N


ATOM
2746
CA
GLU
A
179
74.888
−36.524
13.452
1.00
40.74
C


ATOM
2748
CB
GLU
A
179
75.817
−36.640
12.234
1.00
40.84
C


ATOM
2751
CG
GLU
A
179
75.804
−37.963
11.488
1.00
42.17
C


ATOM
2754
CD
GLU
A
179
76.684
−37.916
10.252
1.00
44.54
C


ATOM
2755
OE1
GLU
A
179
76.170
−38.174
9.143
1.00
45.44
O


ATOM
2756
OE2
GLU
A
179
77.886
−37.597
10.388
1.00
43.08
O


ATOM
2757
C
GLU
A
179
73.543
−37.226
13.217
1.00
39.29
C


ATOM
2758
O
GLU
A
179
72.686
−36.686
12.517
1.00
39.47
O


ATOM
2760
N
LEU
A
180
73.353
−38.414
13.782
1.00
37.43
N


ATOM
2761
CA
LEU
A
180
72.022
−39.025
13.799
1.00
36.29
C


ATOM
2763
CB
LEU
A
180
72.070
−40.507
14.191
1.00
36.48
C


ATOM
2766
CG
LEU
A
180
72.206
−41.541
13.068
1.00
37.27
C


ATOM
2768
CD1
LEU
A
180
72.037
−42.929
13.639
1.00
38.80
C


ATOM
2772
CD2
LEU
A
180
71.195
−41.318
11.949
1.00
37.95
C


ATOM
2776
C
LEU
A
180
71.119
−38.264
14.765
1.00
35.24
C


ATOM
2777
O
LEU
A
180
69.948
−38.028
14.473
1.00
35.11
O


ATOM
2779
N
ALA
A
181
71.667
−37.879
15.912
1.00
33.91
N


ATOM
2780
CA
ALA
A
181
70.915
−37.104
16.892
1.00
33.10
C


ATOM
2782
CB
ALA
A
181
71.797
−36.741
18.078
1.00
32.58
C


ATOM
2786
C
ALA
A
181
70.348
−35.848
16.246
1.00
32.64
C


ATOM
2787
O
ALA
A
181
69.168
−35.539
16.403
1.00
32.91
O


ATOM
2789
N
GLU
A
182
71.195
−35.134
15.513
1.00
32.24
N


ATOM
2790
CA
GLU
A
182
70.777
−33.927
14.814
1.00
32.25
C


ATOM
2792
CB
GLU
A
182
71.965
−33.257
14.116
1.00
33.87
C


ATOM
2795
CG
GLU
A
182
72.871
−32.443
15.053
1.00
38.91
C


ATOM
2798
CD
GLU
A
182
74.082
−31.827
14.340
1.00
46.46
C


ATOM
2799
OE1
GLU
A
182
74.328
−32.150
13.152
1.00
52.06
O


ATOM
2800
OE2
GLU
A
182
74.792
−31.016
14.976
1.00
50.12
O


ATOM
2801
C
GLU
A
182
69.684
−34.241
13.805
1.00
30.14
C


ATOM
2802
O
GLU
A
182
68.708
−33.506
13.714
1.00
30.62
O


ATOM
2804
N
GLN
A
183
69.846
−35.333
13.059
1.00
27.72
N


ATOM
2805
CA
GLN
A
183
68.834
−35.765
12.091
1.00
25.84
C


ATOM
2807
CB
GLN
A
183
69.326
−36.967
11.274
1.00
26.42
C


ATOM
2810
CG
GLN
A
183
70.351
−36.638
10.186
1.00
28.35
C


ATOM
2813
CD
GLN
A
183
69.758
−35.878
9.006
1.00
32.28
C


ATOM
2814
OE1
GLN
A
183
68.614
−36.106
8.604
1.00
36.40
O


ATOM
2815
NE2
GLN
A
183
70.542
−34.971
8.444
1.00
33.91
N


ATOM
2818
C
GLN
A
183
67.513
−36.117
12.774
1.00
23.95
C


ATOM
2819
O
GLN
A
183
66.438
−35.767
12.277
1.00
23.10
O


ATOM
2821
N
VAL
A
184
67.606
−36.805
13.912
1.00
22.75
N


ATOM
2822
CA
VAL
A
184
66.428
−37.238
14.665
1.00
21.10
C


ATOM
2824
CB
VAL
A
184
66.802
−38.184
15.824
1.00
21.25
C


ATOM
2826
CG1
VAL
A
184
65.639
−38.336
16.796
1.00
20.05
C


ATOM
2830
CG2
VAL
A
184
67.229
−39.539
15.286
1.00
19.78
C


ATOM
2834
C
VAL
A
184
65.671
−36.051
15.230
1.00
20.63
C


ATOM
2835
O
VAL
A
184
64.448
−35.967
15.086
1.00
21.39
O


ATOM
2837
N
ASN
A
185
66.397
−35.136
15.862
1.00
19.15
N


ATOM
2838
CA
ASN
A
185
65.781
−33.958
16.450
1.00
19.06
C


ATOM
2840
CB
ASN
A
185
66.798
−33.155
17.265
1.00
19.99
C


ATOM
2843
CG
ASN
A
185
67.350
−33.937
18.462
1.00
21.37
C


ATOM
2844
OD1
ASN
A
185
66.692
−34.825
19.017
1.00
22.33
O


ATOM
2845
ND2
ASN
A
185
68.570
−33.608
18.854
1.00
20.88
N


ATOM
2848
C
ASN
A
185
65.151
−33.082
15.383
1.00
18.27
C


ATOM
2849
O
ASN
A
185
64.051
−32.570
15.567
1.00
18.55
O


ATOM
2851
N
HIS
A
186
65.853
−32.922
14.267
1.00
18.12
N


ATOM
2852
CA
HIS
A
186
65.325
−32.200
13.103
1.00
17.96
C


ATOM
2854
CB
HIS
A
186
66.357
−32.213
11.969
1.00
18.19
C


ATOM
2857
CG
HIS
A
186
66.006
−31.338
10.806
1.00
19.45
C


ATOM
2858
ND1
HIS
A
186
66.056
−29.963
10.866
1.00
22.50
N


ATOM
2860
CE1
HIS
A
186
65.718
−29.461
9.692
1.00
22.65
C


ATOM
2862
NE2
HIS
A
186
65.461
−30.461
8.868
1.00
21.22
N


ATOM
2864
CD2
HIS
A
186
65.642
−31.646
9.538
1.00
21.25
C


ATOM
2866
C
HIS
A
186
64.002
−32.814
12.620
1.00
16.71
C


ATOM
2867
O
HIS
A
186
63.042
−32.089
12.370
1.00
15.76
O


ATOM
2869
N
ALA
A
187
63.956
−34.142
12.509
1.00
15.66
N


ATOM
2870
CA
ALA
A
187
62.749
−34.844
12.052
1.00
15.45
C


ATOM
2872
CB
ALA
A
187
63.045
−36.323
11.783
1.00
13.51
C


ATOM
2876
C
ALA
A
187
61.606
−34.705
13.056
1.00
16.57
C


ATOM
2877
O
ALA
A
187
60.455
−34.462
12.666
1.00
17.16
O


ATOM
2879
N
LEU
A
188
61.923
−34.846
14.344
1.00
17.33
N


ATOM
2880
CA
LEU
A
188
60.916
−34.712
15.402
1.00
18.39
C


ATOM
2882
CB
LEU
A
188
61.440
−35.277
16.725
1.00
18.54
C


ATOM
2885
CG
LEU
A
188
61.685
−36.799
16.774
1.00
20.88
C


ATOM
2887
CD1
LEU
A
188
62.370
−37.204
18.079
1.00
14.03
C


ATOM
2891
CD2
LEU
A
188
60.389
−37.601
16.583
1.00
18.90
C


ATOM
2895
C
LEU
A
188
60.425
−33.270
15.586
1.00
19.27
C


ATOM
2896
O
LEU
A
188
59.314
−33.059
16.053
1.00
19.68
O


ATOM
2898
N
GLU
A
189
61.243
−32.287
15.214
1.00
20.30
N


ATOM
2899
CA
GLU
A
189
60.827
−30.881
15.238
1.00
21.36
C


ATOM
2901
CB
GLU
A
189
62.026
−29.962
14.957
1.00
21.99
C


ATOM
2904
CG
GLU
A
189
61.699
−28.470
14.954
1.00
26.04
C


ATOM
2907
CD
GLU
A
189
62.841
−27.608
14.433
1.00
32.64
C


ATOM
2908
OE1
GLU
A
189
63.272
−27.809
13.276
1.00
35.76
O


ATOM
2909
OE2
GLU
A
189
63.297
−26.713
15.181
1.00
37.28
O


ATOM
2910
C
GLU
A
189
59.731
−30.618
14.206
1.00
21.74
C


ATOM
2911
O
GLU
A
189
58.759
−29.913
14.484
1.00
22.33
O


ATOM
2913
N
LEU
A
190
59.916
−31.172
13.013
1.00
21.35
N


ATOM
2914
CA
LEU
A
190
58.983
−31.008
11.911
1.00
21.78
C


ATOM
2916
CB
LEU
A
190
58.922
−29.537
11.472
1.00
22.20
C


ATOM
2919
CG
LEU
A
190
57.780
−29.113
10.548
1.00
24.65
C


ATOM
2921
CD1
LEU
A
190
56.457
−29.141
11.279
1.00
20.76
C


ATOM
2925
CD2
LEU
A
190
58.036
−27.722
9.966
1.00
24.14
C


ATOM
2929
C
LEU
A
190
59.466
−31.894
10.756
1.00
21.36
C


ATOM
2930
O
LEU
A
190
60.628
−31.830
10.375
1.00
21.03
O


ATOM
2932
N
PRO
A
191
58.584
−32.745
10.213
1.00
21.29
N


ATOM
2933
CA
PRO
A
191
59.000
−33.644
9.145
1.00
20.66
C


ATOM
2935
CB
PRO
A
191
57.850
−34.642
9.072
1.00
20.81
C


ATOM
2938
CG
PRO
A
191
56.679
−33.890
9.530
1.00
20.77
C


ATOM
2941
CD
PRO
A
191
57.159
−32.911
10.542
1.00
20.89
C


ATOM
2944
C
PRO
A
191
59.175
−32.932
7.804
1.00
21.01
C


ATOM
2945
O
PRO
A
191
58.554
−31.892
7.561
1.00
20.86
O


ATOM
2946
N
LEU
A
192
60.004
−33.515
6.942
1.00
20.69
N


ATOM
2947
CA
LEU
A
192
60.315
−32.939
5.638
1.00
20.28
C


ATOM
2949
CB
LEU
A
192
61.095
−33.940
4.789
1.00
20.87
C


ATOM
2952
CG
LEU
A
192
62.521
−34.245
5.250
1.00
24.52
C


ATOM
2954
CD1
LEU
A
192
63.020
−35.527
4.593
1.00
24.37
C


ATOM
2958
CD2
LEU
A
192
63.449
−33.072
4.958
1.00
23.68
C


ATOM
2962
C
LEU
A
192
59.070
−32.523
4.878
1.00
19.68
C


ATOM
2963
O
LEU
A
192
59.019
−31.429
4.329
1.00
20.16
O


ATOM
2965
N
HIS
A
193
58.060
−33.388
4.853
1.00
19.46
N


ATOM
2966
CA
HIS
A
193
56.841
−33.102
4.087
1.00
19.61
C


ATOM
2968
CB
HIS
A
193
55.856
−34.273
4.164
1.00
18.91
C


ATOM
2971
CG
HIS
A
193
54.730
−34.176
3.183
1.00
18.53
C


ATOM
2972
ND1
HIS
A
193
54.934
−34.015
1.829
1.00
16.78
N


ATOM
2974
CE1
HIS
A
193
53.767
−33.955
1.216
1.00
16.41
C


ATOM
2976
NE2
HIS
A
193
52.814
−34.076
2.121
1.00
17.58
N


ATOM
2978
CD2
HIS
A
193
53.390
−34.217
3.359
1.00
21.13
C


ATOM
2980
C
HIS
A
193
56.136
−31.799
4.503
1.00
20.58
C


ATOM
2981
O
HIS
A
193
55.449
−31.194
3.690
1.00
20.81
O


ATOM
2983
N
ARG
A
194
56.310
−31.378
5.756
1.00
21.61
N


ATOM
2984
CA
ARG
A
194
55.652
−30.177
6.275
1.00
22.78
C


ATOM
2986
CB
ARG
A
194
55.055
−30.463
7.645
1.00
22.74
C


ATOM
2989
CG
ARG
A
194
53.936
−31.466
7.599
1.00
24.13
C


ATOM
2992
CD
ARG
A
194
53.244
−31.577
8.937
1.00
25.19
C


ATOM
2995
NE
ARG
A
194
52.115
−32.498
8.878
1.00
26.72
N


ATOM
2997
CZ
ARG
A
194
51.461
−32.967
9.939
1.00
24.95
C


ATOM
2998
NH1
ARG
A
194
51.811
−32.609
11.169
1.00
25.40
N


ATOM
3001
NH2
ARG
A
194
50.447
−33.799
9.763
1.00
23.76
N


ATOM
3004
C
ARG
A
194
56.579
−28.971
6.387
1.00
23.86
C


ATOM
3005
O
ARG
A
194
56.105
−27.853
6.626
1.00
24.87
O


ATOM
3007
N
ARG
A
195
57.884
−29.195
6.209
1.00
22.92
N


ATOM
3008
CA
ARG
A
195
58.872
−28.121
6.258
1.00
21.47
C


ATOM
3010
CB
ARG
A
195
60.222
−28.670
6.706
1.00
21.67
C


ATOM
3013
CG
ARG
A
195
61.179
−27.620
7.232
1.00
21.84
C


ATOM
3016
CD
ARG
A
195
62.519
−28.226
7.535
1.00
20.35
C


ATOM
3019
NE
ARG
A
195
62.447
−29.168
8.648
1.00
19.52
N


ATOM
3021
CZ
ARG
A
195
62.561
−28.842
9.934
1.00
21.51
C


ATOM
3022
NH1
ARG
A
195
62.762
−27.580
10.310
1.00
20.60
N


ATOM
3025
NH2
ARG
A
195
62.476
−29.793
10.860
1.00
23.45
N


ATOM
3028
C
ARG
A
195
59.018
−27.465
4.895
1.00
20.90
C


ATOM
3029
O
ARG
A
195
58.986
−28.146
3.862
1.00
21.00
O


ATOM
3031
N
THR
A
196
59.187
−26.141
4.897
1.00
20.22
N


ATOM
3032
CA
THR
A
196
59.382
−25.379
3.661
1.00
18.71
C


ATOM
3034
CB
THR
A
196
59.332
−23.880
3.913
1.00
18.34
C


ATOM
3036
OG1
THR
A
196
60.229
−23.559
4.977
1.00
18.15
O


ATOM
3038
CG2
THR
A
196
57.922
−23.435
4.282
1.00
16.30
C


ATOM
3042
C
THR
A
196
60.731
−25.696
3.020
1.00
19.40
C


ATOM
3043
O
THR
A
196
61.688
−26.131
3.696
1.00
19.33
O


ATOM
3045
N
GLN
A
197
60.809
−25.448
1.716
1.00
18.56
N


ATOM
3046
CA
GLN
A
197
61.934
−25.917
0.920
1.00
18.45
C


ATOM
3048
CB
GLN
A
197
61.623
−25.783
−0.576
1.00
19.04
C


ATOM
3051
CG
GLN
A
197
62.363
−26.781
−1.484
1.00
20.45
C


ATOM
3054
CD
GLN
A
197
63.690
−26.254
−2.029
1.00
23.06
C


ATOM
3055
OE1
GLN
A
197
64.326
−25.377
−1.435
1.00
22.28
O


ATOM
3056
NE2
GLN
A
197
64.113
−26.798
−3.168
1.00
23.25
N


ATOM
3059
C
GLN
A
197
63.250
−25.217
1.292
1.00
18.75
C


ATOM
3060
O
GLN
A
197
64.264
−25.899
1.487
1.00
20.24
O


ATOM
3062
N
ARG
A
198
63.238
−23.885
1.415
1.00
16.75
N


ATOM
3063
CA
ARG
A
198
64.460
−23.132
1.751
1.00
16.08
C


ATOM
3065
CB
ARG
A
198
64.243
−21.626
1.617
1.00
14.41
C


ATOM
3068
CG
ARG
A
198
64.010
−21.157
0.186
1.00
16.34
C


ATOM
3071
CD
ARG
A
198
65.263
−21.285
−0.701
1.00
18.13
C


ATOM
3074
NE
ARG
A
198
65.406
−22.597
−1.339
1.00
18.36
N


ATOM
3076
CZ
ARG
A
198
66.411
−22.933
−2.149
1.00
17.71
C


ATOM
3077
NH1
ARG
A
198
67.374
−22.061
−2.424
1.00
19.46
N


ATOM
3080
NH2
ARG
A
198
66.465
−24.148
−2.689
1.00
17.52
N


ATOM
3083
C
ARG
A
198
65.010
−23.439
3.142
1.00
17.39
C


ATOM
3084
O
ARG
A
198
66.231
−23.523
3.319
1.00
18.40
O


ATOM
3086
N
LEU
A
199
64.126
−23.602
4.125
1.00
18.03
N


ATOM
3087
CA
LEU
A
199
64.551
−23.907
5.491
1.00
18.94
C


ATOM
3089
CB
LEU
A
199
63.361
−23.914
6.440
1.00
20.48
C


ATOM
3092
CG
LEU
A
199
62.877
−22.553
6.916
1.00
23.96
C


ATOM
3094
CD1
LEU
A
199
61.583
−22.719
7.705
1.00
24.08
C


ATOM
3098
CD2
LEU
A
199
63.961
−21.896
7.753
1.00
22.94
C


ATOM
3102
C
LEU
A
199
65.252
−25.253
5.568
1.00
18.95
C


ATOM
3103
O
LEU
A
199
66.288
−25.384
6.207
1.00
19.37
O


ATOM
3105
N
GLU
A
200
64.678
−26.257
4.918
1.00
19.33
N


ATOM
3106
CA
GLU
A
200
65.324
−27.562
4.822
1.00
19.05
C


ATOM
3108
CB
GLU
A
200
64.409
−28.552
4.102
1.00
18.90
C


ATOM
3111
CG
GLU
A
200
64.942
−29.958
3.983
1.00
18.52
C


ATOM
3114
CD
GLU
A
200
65.404
−30.546
5.299
1.00
24.43
C


ATOM
3115
OE1
GLU
A
200
64.818
−30.236
6.361
1.00
24.97
O


ATOM
3116
OE2
GLU
A
200
66.360
−31.343
5.264
1.00
29.77
O


ATOM
3117
C
GLU
A
200
66.669
−27.471
4.103
1.00
19.17
C


ATOM
3118
O
GLU
A
200
67.625
−28.151
4.489
1.00
19.91
O


ATOM
3120
N
ALA
A
201
66.748
−26.631
3.071
1.00
18.52
N


ATOM
3121
CA
ALA
A
201
67.988
−26.490
2.306
1.00
18.74
C


ATOM
3123
CB
ALA
A
201
67.765
−25.669
1.041
1.00
17.65
C


ATOM
3127
C
ALA
A
201
69.100
−25.874
3.151
1.00
19.12
C


ATOM
3128
O
ALA
A
201
70.237
−26.357
3.136
1.00
19.39
O


ATOM
3130
N
VAL
A
202
68.781
−24.812
3.886
1.00
18.58
N


ATOM
3131
CA
VAL
A
202
69.795
−24.140
4.686
1.00
18.09
C


ATOM
3133
CB
VAL
A
202
69.290
−22.805
5.297
1.00
18.62
C


ATOM
3135
CG1
VAL
A
202
68.341
−23.045
6.453
1.00
17.88
C


ATOM
3139
CG2
VAL
A
202
70.468
−21.968
5.752
1.00
17.48
C


ATOM
3143
C
VAL
A
202
70.297
−25.076
5.776
1.00
17.71
C


ATOM
3144
O
VAL
A
202
71.491
−25.105
6.074
1.00
19.28
O


ATOM
3146
N
TRP
A
203
69.387
−25.864
6.340
1.00
16.99
N


ATOM
3147
CA
TRP
A
203
69.738
−26.815
7.378
1.00
16.69
C


ATOM
3149
CB
TRP
A
203
68.482
−27.357
8.073
1.00
17.28
C


ATOM
3152
CG
TRP
A
203
68.807
−28.148
9.286
1.00
17.11
C


ATOM
3153
CD1
TRP
A
203
68.935
−27.678
10.554
1.00
17.84
C


ATOM
3155
NE1
TRP
A
203
69.271
−28.704
11.406
1.00
17.21
N


ATOM
3157
CE2
TRP
A
203
69.373
−29.861
10.685
1.00
16.55
C


ATOM
3158
CD2
TRP
A
203
69.086
−29.547
9.344
1.00
17.54
C


ATOM
3159
CE3
TRP
A
203
69.126
−30.565
8.393
1.00
19.66
C


ATOM
3161
CZ3
TRP
A
203
69.436
−31.854
8.807
1.00
21.37
C


ATOM
3163
CH2
TRP
A
203
69.712
−32.133
10.143
1.00
21.02
C


ATOM
3165
CZ2
TRP
A
203
69.686
−31.150
11.097
1.00
20.22
C


ATOM
3167
C
TRP
A
203
70.564
−27.959
6.799
1.00
16.20
C


ATOM
3168
O
TRP
A
203
71.653
−28.235
7.288
1.00
16.09
O


ATOM
3170
N
SER
A
204
70.053
−28.610
5.756
1.00
16.35
N


ATOM
3171
CA
SER
A
204
70.722
−29.792
5.179
1.00
16.36
C


ATOM
3173
CB
SER
A
204
69.856
−30.454
4.102
1.00
16.65
C


ATOM
3176
OG
SER
A
204
68.755
−31.118
4.693
1.00
17.66
O


ATOM
3178
C
SER
A
204
72.098
−29.489
4.604
1.00
14.94
C


ATOM
3179
O
SER
A
204
72.999
−30.329
4.678
1.00
14.23
O


ATOM
3181
N
ILE
A
205
72.268
−28.304
4.028
1.00
14.13
N


ATOM
3182
CA
ILE
A
205
73.580
−27.919
3.506
1.00
14.35
C


ATOM
3184
CB
ILE
A
205
73.544
−26.563
2.778
1.00
14.82
C


ATOM
3186
CG1
ILE
A
205
72.869
−26.729
1.412
1.00
14.39
C


ATOM
3189
CD1
ILE
A
205
72.455
−25.425
0.755
1.00
11.85
C


ATOM
3193
CG2
ILE
A
205
74.954
−26.011
2.614
1.00
12.68
C


ATOM
3197
C
ILE
A
205
74.619
−27.914
4.633
1.00
13.92
C


ATOM
3198
O
ILE
A
205
75.690
−28.484
4.489
1.00
11.72
O


ATOM
3200
N
GLU
A
206
74.273
−27.307
5.765
1.00
15.11
N


ATOM
3201
CA
GLU
A
206
75.152
−27.312
6.938
1.00
15.74
C


ATOM
3203
CB
GLU
A
206
74.561
−26.447
8.059
1.00
16.58
C


ATOM
3206
CG
GLU
A
206
75.451
−26.307
9.311
1.00
20.35
C


ATOM
3209
CD
GLU
A
206
76.720
−25.486
9.089
1.00
23.18
C


ATOM
3210
OE1
GLU
A
206
76.929
−24.975
7.966
1.00
26.36
O


ATOM
3211
OE2
GLU
A
206
77.507
−25.351
10.057
1.00
20.56
O


ATOM
3212
C
GLU
A
206
75.433
−28.726
7.450
1.00
14.77
C


ATOM
3213
O
GLU
A
206
76.559
−29.028
7.848
1.00
14.90
O


ATOM
3215
N
ALA
A
207
74.419
−29.587
7.440
1.00
13.71
N


ATOM
3216
CA
ALA
A
207
74.599
−30.980
7.844
1.00
13.82
C


ATOM
3218
CB
ALA
A
207
73.270
−31.683
7.919
1.00
12.84
C


ATOM
3222
C
ALA
A
207
75.526
−31.721
6.881
1.00
15.05
C


ATOM
3223
O
ALA
A
207
76.524
−32.325
7.294
1.00
13.97
O


ATOM
3225
N
TYR
A
208
75.189
−31.665
5.595
1.00
16.57
N


ATOM
3226
CA
TYR
A
208
75.949
−32.371
4.564
1.00
17.16
C


ATOM
3228
CB
TYR
A
208
75.304
−32.148
3.188
1.00
16.84
C


ATOM
3231
CG
TYR
A
208
75.655
−33.205
2.166
1.00
18.47
C


ATOM
3232
CD1
TYR
A
208
75.244
−34.519
2.332
1.00
20.52
C


ATOM
3234
CE1
TYR
A
208
75.564
−35.493
1.406
1.00
22.10
C


ATOM
3236
CZ
TYR
A
208
76.295
−35.158
0.292
1.00
20.93
C


ATOM
3237
OH
TYR
A
208
76.614
−36.127
−0.625
1.00
26.11
O


ATOM
3239
CE2
TYR
A
208
76.715
−33.863
0.099
1.00
21.06
C


ATOM
3241
CD2
TYR
A
208
76.392
−32.892
1.032
1.00
20.04
C


ATOM
3243
C
TYR
A
208
77.408
−31.911
4.564
1.00
17.97
C


ATOM
3244
O
TYR
A
208
78.330
−32.723
4.516
1.00
17.30
O


ATOM
3246
N
ARG
A
209
77.598
−30.598
4.637
1.00
19.31
N


ATOM
3247
CA
ARG
A
209
78.924
−29.981
4.664
1.00
20.26
C


ATOM
3249
CB
ARG
A
209
78.772
−28.486
4.944
1.00
20.16
C


ATOM
3252
CG
ARG
A
209
80.011
−27.681
4.695
1.00
23.03
C


ATOM
3255
CD
ARG
A
209
79.957
−26.376
5.426
1.00
24.02
C


ATOM
3258
NE
ARG
A
209
79.057
−25.427
4.788
1.00
22.04
N


ATOM
3260
CZ
ARG
A
209
79.416
−24.509
3.887
1.00
21.79
C


ATOM
3261
NH1
ARG
A
209
80.671
−24.395
3.461
1.00
15.73
N


ATOM
3264
NH2
ARG
A
209
78.493
−23.692
3.401
1.00
27.01
N


ATOM
3267
C
ARG
A
209
79.873
−30.606
5.701
1.00
21.41
C


ATOM
3268
O
ARG
A
209
81.064
−30.753
5.437
1.00
22.06
O


ATOM
3270
N
LYS
A
210
79.340
−30.960
6.871
1.00
22.53
N


ATOM
3271
CA
LYS
A
210
80.119
−31.572
7.952
1.00
23.50
C


ATOM
3273
CB
LYS
A
210
79.377
−31.439
9.286
1.00
23.64
C


ATOM
3276
CG
LYS
A
210
79.234
−30.016
9.809
1.00
24.69
C


ATOM
3279
CD
LYS
A
210
78.235
−29.961
10.975
1.00
27.65
C


ATOM
3282
CE
LYS
A
210
77.837
−28.528
11.324
1.00
29.43
C


ATOM
3285
NZ
LYS
A
210
76.521
−28.453
12.020
1.00
27.05
N


ATOM
3289
C
LYS
A
210
80.449
−33.056
7.731
1.00
25.07
C


ATOM
3290
O
LYS
A
210
81.412
−33.558
8.310
1.00
24.91
O


ATOM
3292
N
LYS
A
211
79.646
−33.761
6.932
1.00
26.97
N


ATOM
3293
CA
LYS
A
211
79.879
−35.187
6.671
1.00
28.62
C


ATOM
3295
CB
LYS
A
211
78.755
−35.804
5.829
1.00
29.48
C


ATOM
3298
CG
LYS
A
211
77.364
−35.837
6.474
1.00
32.60
C


ATOM
3301
CD
LYS
A
211
76.459
−36.843
5.737
1.00
37.94
C


ATOM
3304
CE
LYS
A
211
74.963
−36.588
5.964
1.00
42.00
C


ATOM
3307
NZ
LYS
A
211
74.515
−36.865
7.362
1.00
43.99
N


ATOM
3311
C
LYS
A
211
81.198
−35.368
5.934
1.00
29.70
C


ATOM
3312
O
LYS
A
211
81.512
−34.609
5.015
1.00
30.26
O


ATOM
3314
N
GLU
A
212
81.962
−36.380
6.335
1.00
30.49
N


ATOM
3315
CA
GLU
A
212
83.295
−36.608
5.785
1.00
30.93
C


ATOM
3317
CB
GLU
A
212
84.095
−37.536
6.702
1.00
31.80
C


ATOM
3320
CG
GLU
A
212
85.518
−37.811
6.217
1.00
35.83
C


ATOM
3323
CD
GLU
A
212
86.410
−38.439
7.279
1.00
40.51
C


ATOM
3324
OE1
GLU
A
212
86.043
−38.408
8.476
1.00
42.71
O


ATOM
3325
OE2
GLU
A
212
87.486
−38.960
6.907
1.00
40.43
O


ATOM
3326
C
GLU
A
212
83.246
−37.184
4.372
1.00
30.27
C


ATOM
3327
O
GLU
A
212
84.097
−36.868
3.540
1.00
31.06
O


ATOM
3329
N
ASP
A
213
82.252
−38.023
4.103
1.00
28.55
N


ATOM
3330
CA
ASP
A
213
82.118
−38.656
2.796
1.00
27.73
C


ATOM
3332
CB
ASP
A
213
81.807
−40.150
2.971
1.00
28.70
C


ATOM
3335
CG
ASP
A
213
80.425
−40.408
3.558
1.00
31.61
C


ATOM
3336
OD1
ASP
A
213
79.835
−39.498
4.185
1.00
34.37
O


ATOM
3337
OD2
ASP
A
213
79.933
−41.542
3.389
1.00
37.46
O


ATOM
3338
C
ASP
A
213
81.057
−37.969
1.918
1.00
25.89
C


ATOM
3339
O
ASP
A
213
80.453
−38.602
1.045
1.00
25.29
O


ATOM
3341
N
ALA
A
214
80.840
−36.675
2.151
1.00
23.65
N


ATOM
3342
CA
ALA
A
214
79.869
−35.899
1.381
1.00
21.07
C


ATOM
3344
CB
ALA
A
214
79.659
−34.538
2.015
1.00
20.78
C


ATOM
3348
C
ALA
A
214
80.341
−35.735
−0.055
1.00
18.82
C


ATOM
3349
O
ALA
A
214
81.521
−35.517
−0.298
1.00
18.38
O


ATOM
3351
N
ASN
A
215
79.422
−35.848
−1.007
1.00
17.54
N


ATOM
3352
CA
ASN
A
215
79.768
−35.635
−2.408
1.00
16.55
C


ATOM
3354
CB
ASN
A
215
78.649
−36.133
−3.326
1.00
15.72
C


ATOM
3357
CG
ASN
A
215
79.015
−36.034
−4.790
1.00
15.82
C


ATOM
3358
OD1
ASN
A
215
79.297
−34.946
−5.306
1.00
12.43
O


ATOM
3359
ND2
ASN
A
215
79.023
−37.168
−5.468
1.00
16.94
N


ATOM
3362
C
ASN
A
215
80.080
−34.150
−2.639
1.00
15.79
C


ATOM
3363
O
ASN
A
215
79.210
−33.292
−2.499
1.00
14.49
O


ATOM
3365
N
GLN
A
216
81.332
−33.857
−2.978
1.00
16.27
N


ATOM
3366
CA
GLN
A
216
81.806
−32.468
−3.054
1.00
16.53
C


ATOM
3368
CB
GLN
A
216
83.336
−32.423
−3.113
1.00
16.76
C


ATOM
3371
CG
GLN
A
216
84.028
−33.071
−1.905
1.00
20.08
C


ATOM
3374
CD
GLN
A
216
83.592
−32.471
−0.569
1.00
23.47
C


ATOM
3375
OE1
GLN
A
216
84.024
−31.378
−0.189
1.00
24.87
O


ATOM
3376
NE2
GLN
A
216
82.739
−33.194
0.153
1.00
23.11
N


ATOM
3379
C
GLN
A
216
81.205
−31.695
−4.227
1.00
15.89
C


ATOM
3380
O
GLN
A
216
81.028
−30.478
−4.140
1.00
16.63
O


ATOM
3382
N
VAL
A
217
80.881
−32.401
−5.309
1.00
15.02
N


ATOM
3383
CA
VAL
A
217
80.212
−31.790
−6.454
1.00
13.80
C


ATOM
3385
CB
VAL
A
217
80.154
−32.742
−7.662
1.00
14.59
C


ATOM
3387
CG1
VAL
A
217
79.177
−32.209
−8.726
1.00
13.00
C


ATOM
3391
CG2
VAL
A
217
81.555
−32.954
−8.240
1.00
9.07
C


ATOM
3395
C
VAL
A
217
78.799
−31.397
−6.084
1.00
13.21
C


ATOM
3396
O
VAL
A
217
78.361
−30.287
−6.364
1.00
13.41
O


ATOM
3398
N
LEU
A
218
78.089
−32.323
−5.453
1.00
13.47
N


ATOM
3399
CA
LEU
A
218
76.721
−32.080
−5.008
1.00
13.08
C


ATOM
3401
CB
LEU
A
218
76.120
−33.368
−4.434
1.00
12.26
C


ATOM
3404
CG
LEU
A
218
74.640
−33.367
−4.039
1.00
12.25
C


ATOM
3406
CD1
LEU
A
218
73.762
−32.784
−5.134
1.00
6.53
C


ATOM
3410
CD2
LEU
A
218
74.203
−34.781
−3.689
1.00
5.69
C


ATOM
3414
C
LEU
A
218
76.682
−30.946
−3.972
1.00
13.78
C


ATOM
3415
O
LEU
A
218
75.867
−30.021
−4.076
1.00
12.92
O


ATOM
3417
N
LEU
A
219
77.583
−31.014
−2.991
1.00
13.86
N


ATOM
3418
CA
LEU
A
219
77.710
−29.965
−1.981
1.00
13.37
C


ATOM
3420
CB
LEU
A
219
78.860
−30.279
−1.015
1.00
13.97
C


ATOM
3423
CG
LEU
A
219
79.174
−29.259
0.094
1.00
13.38
C


ATOM
3425
CD1
LEU
A
219
77.910
−28.906
0.910
1.00
7.90
C


ATOM
3429
CD2
LEU
A
219
80.281
−29.796
0.996
1.00
8.87
C


ATOM
3433
C
LEU
A
219
77.946
−28.610
−2.629
1.00
13.03
C


ATOM
3434
O
LEU
A
219
77.262
−27.640
−2.307
1.00
12.64
O


ATOM
3436
N
GLU
A
220
78.905
−28.548
−3.549
1.00
13.28
N


ATOM
3437
CA
GLU
A
220
79.276
−27.269
−4.171
1.00
14.02
C


ATOM
3439
CB
GLU
A
220
80.548
−27.408
−5.022
1.00
13.85
C


ATOM
3442
CG
GLU
A
220
81.027
−26.078
−5.618
1.00
14.99
C


ATOM
3445
CD
GLU
A
220
82.386
−26.155
−6.305
1.00
13.51
C


ATOM
3446
OE1
GLU
A
220
82.967
−27.262
−6.413
1.00
10.78
O


ATOM
3447
OE2
GLU
A
220
82.875
−25.084
−6.731
1.00
15.71
O


ATOM
3448
C
GLU
A
220
78.130
−26.668
−4.995
1.00
14.29
C


ATOM
3449
O
GLU
A
220
77.928
−25.455
−4.982
1.00
15.62
O


ATOM
3451
N
LEU
A
221
77.380
−27.513
−5.696
1.00
14.42
N


ATOM
3452
CA
LEU
A
221
76.221
−27.059
−6.471
1.00
14.42
C


ATOM
3454
CB
LEU
A
221
75.672
−28.214
−7.315
1.00
14.48
C


ATOM
3457
CG
LEU
A
221
74.489
−27.918
−8.241
1.00
13.15
C


ATOM
3459
CD1
LEU
A
221
74.895
−27.012
−9.386
1.00
3.14
C


ATOM
3463
CD2
LEU
A
221
73.919
−29.218
−8.761
1.00
11.66
C


ATOM
3467
C
LEU
A
221
75.122
−26.503
−5.557
1.00
14.55
C


ATOM
3468
O
LEU
A
221
74.581
−25.419
−5.804
1.00
14.29
O


ATOM
3470
N
ALA
A
222
74.805
−27.246
−4.499
1.00
14.74
N


ATOM
3471
CA
ALA
A
222
73.802
−26.816
−3.520
1.00
14.77
C


ATOM
3473
CB
ALA
A
222
73.661
−27.845
−2.404
1.00
13.62
C


ATOM
3477
C
ALA
A
222
74.122
−25.439
−2.937
1.00
15.47
C


ATOM
3478
O
ALA
A
222
73.217
−24.628
−2.736
1.00
17.17
O


ATOM
3480
N
ILE
A
223
75.401
−25.171
−2.680
1.00
15.03
N


ATOM
3481
CA
ILE
A
223
75.816
−23.883
−2.131
1.00
14.70
C


ATOM
3483
CB
ILE
A
223
77.262
−23.931
−1.578
1.00
14.73
C


ATOM
3485
CG1
ILE
A
223
77.343
−24.885
−0.377
1.00
14.63
C


ATOM
3488
CD1
ILE
A
223
78.751
−25.300
0.007
1.00
9.76
C


ATOM
3492
CG2
ILE
A
223
77.720
−22.548
−1.150
1.00
12.34
C


ATOM
3496
C
ILE
A
223
75.677
−22.787
−3.191
1.00
15.33
C


ATOM
3497
O
ILE
A
223
75.057
−21.743
−2.936
1.00
15.78
O


ATOM
3499
N
LEU
A
224
76.249
−23.026
−4.372
1.00
15.28
N


ATOM
3500
CA
LEU
A
224
76.109
−22.101
−5.506
1.00
15.28
C


ATOM
3502
CB
LEU
A
224
76.693
−22.695
−6.792
1.00
15.38
C


ATOM
3505
CG
LEU
A
224
78.067
−22.187
−7.225
1.00
18.98
C


ATOM
3507
CD1
LEU
A
224
79.101
−22.377
−6.128
1.00
20.71
C


ATOM
3511
CD2
LEU
A
224
78.509
−22.885
−8.509
1.00
20.84
C


ATOM
3515
C
LEU
A
224
74.650
−21.748
−5.747
1.00
15.02
C


ATOM
3516
O
LEU
A
224
74.298
−20.569
−5.825
1.00
15.33
O


ATOM
3518
N
ASP
A
225
73.807
−22.776
−5.838
1.00
14.27
N


ATOM
3519
CA
ASP
A
225
72.402
−22.593
−6.200
1.00
13.87
C


ATOM
3521
CB
ASP
A
225
71.752
−23.931
−6.558
1.00
13.21
C


ATOM
3524
CG
ASP
A
225
70.416
−23.759
−7.228
1.00
11.77
C


ATOM
3525
OD1
ASP
A
225
70.381
−23.575
−8.460
1.00
15.81
O


ATOM
3526
OD2
ASP
A
225
69.386
−23.818
−6.529
1.00
14.91
O


ATOM
3527
C
ASP
A
225
71.591
−21.893
−5.111
1.00
13.91
C


ATOM
3528
O
ASP
A
225
70.694
−21.119
−5.422
1.00
14.90
O


ATOM
3530
N
TYR
A
226
71.905
−22.155
−3.845
1.00
13.95
N


ATOM
3531
CA
TYR
A
226
71.197
−21.510
−2.742
1.00
15.40
C


ATOM
3533
CB
TYR
A
226
71.577
−22.130
−1.392
1.00
16.25
C


ATOM
3536
CG
TYR
A
226
70.802
−21.542
−0.228
1.00
16.29
C


ATOM
3537
CD1
TYR
A
226
71.203
−20.353
0.379
1.00
17.70
C


ATOM
3539
CE1
TYR
A
226
70.487
−19.803
1.439
1.00
16.58
C


ATOM
3541
CZ
TYR
A
226
69.361
−20.447
1.901
1.00
18.63
C


ATOM
3542
OH
TYR
A
226
68.638
−19.921
2.942
1.00
16.92
O


ATOM
3544
CE2
TYR
A
226
68.946
−21.628
1.316
1.00
19.18
C


ATOM
3546
CD2
TYR
A
226
69.665
−22.165
0.255
1.00
18.06
C


ATOM
3548
C
TYR
A
226
71.482
−20.014
−2.712
1.00
16.97
C


ATOM
3549
O
TYR
A
226
70.561
−19.205
−2.557
1.00
17.79
O


ATOM
3551
N
ASN
A
227
72.758
−19.655
−2.851
1.00
17.52
N


ATOM
3552
CA
ASN
A
227
73.159
−18.252
−2.845
1.00
17.45
C


ATOM
3554
CB
ASN
A
227
74.679
−18.125
−2.710
1.00
17.25
C


ATOM
3557
CG
ASN
A
227
75.161
−18.463
−1.318
1.00
14.98
C


ATOM
3558
OD1
ASN
A
227
74.378
−18.487
−0.366
1.00
16.17
O


ATOM
3559
ND2
ASN
A
227
76.446
−18.733
−1.188
1.00
11.54
N


ATOM
3562
C
ASN
A
227
72.674
−17.492
−4.064
1.00
17.98
C


ATOM
3563
O
ASN
A
227
72.431
−16.289
−3.985
1.00
18.37
O


ATOM
3565
N
MET
A
228
72.528
−18.190
−5.186
1.00
19.19
N


ATOM
3566
CA
MET
A
228
72.028
−17.568
−6.405
1.00
20.00
C


ATOM
3568
CB
MET
A
228
72.115
−18.531
−7.583
1.00
20.90
C


ATOM
3571
CG
MET
A
228
71.478
−17.996
−8.861
1.00
24.50
C


ATOM
3574
SD
MET
A
228
71.047
−19.298
−10.019
1.00
32.87
S


ATOM
3575
CE
MET
A
228
69.767
−20.178
−9.117
1.00
20.31
C


ATOM
3579
C
MET
A
228
70.588
−17.149
−6.196
1.00
19.82
C


ATOM
3580
O
MET
A
228
70.220
−16.005
−6.464
1.00
20.14
O


ATOM
3582
N
ILE
A
229
69.781
−18.089
−5.718
1.00
19.86
N


ATOM
3583
CA
ILE
A
229
68.369
−17.837
−5.445
1.00
19.70
C


ATOM
3585
CB
ILE
A
229
67.642
−19.129
−4.991
1.00
19.09
C


ATOM
3587
CG1
ILE
A
229
67.547
−20.114
−6.149
1.00
17.67
C


ATOM
3590
CD1
ILE
A
229
67.026
−21.465
−5.740
1.00
18.73
C


ATOM
3594
CG2
ILE
A
229
66.247
−18.822
−4.481
1.00
15.65
C


ATOM
3598
C
ILE
A
229
68.218
−16.745
−4.380
1.00
21.11
C


ATOM
3599
O
ILE
A
229
67.354
−15.872
−4.493
1.00
22.14
O


ATOM
3601
N
GLN
A
230
69.066
−16.788
−3.357
1.00
21.50
N


ATOM
3602
CA
GLN
A
230
69.041
−15.768
−2.321
1.00
22.18
C


ATOM
3604
CB
GLN
A
230
70.138
−16.003
−1.288
1.00
22.21
C


ATOM
3607
CG
GLN
A
230
69.965
−15.131
−0.066
1.00
24.09
C


ATOM
3610
CD
GLN
A
230
70.892
−15.507
1.053
1.00
25.30
C


ATOM
3611
OE1
GLN
A
230
72.040
−15.077
1.081
1.00
26.06
O


ATOM
3612
NE2
GLN
A
230
70.393
−16.298
1.999
1.00
23.59
N


ATOM
3615
C
GLN
A
230
69.208
−14.369
−2.900
1.00
22.26
C


ATOM
3616
O
GLN
A
230
68.591
−13.421
−2.422
1.00
23.58
O


ATOM
3618
N
SER
A
231
70.052
−14.232
−3.915
1.00
21.90
N


ATOM
3619
CA
SER
A
231
70.283
−12.918
−4.514
1.00
21.86
C


ATOM
3621
CB
SER
A
231
71.611
−12.883
−5.287
1.00
21.12
C


ATOM
3624
OG
SER
A
231
71.491
−13.518
−6.537
1.00
24.84
O


ATOM
3626
C
SER
A
231
69.097
−12.496
−5.392
1.00
20.18
C


ATOM
3627
O
SER
A
231
68.885
−11.306
−5.629
1.00
20.45
O


ATOM
3629
N
VAL
A
232
68.326
−13.470
−5.869
1.00
19.16
N


ATOM
3630
CA
VAL
A
232
67.046
−13.171
−6.516
1.00
18.13
C


ATOM
3632
CB
VAL
A
232
66.445
−14.397
−7.236
1.00
18.01
C


ATOM
3634
CG1
VAL
A
232
65.037
−14.087
−7.753
1.00
15.57
C


ATOM
3638
CG2
VAL
A
232
67.360
−14.834
−8.373
1.00
11.93
C


ATOM
3642
C
VAL
A
232
66.061
−12.632
−5.479
1.00
18.11
C


ATOM
3643
O
VAL
A
232
65.354
−11.665
−5.747
1.00
17.35
O


ATOM
3645
N
TYR
A
233
66.032
−13.244
−4.294
1.00
18.30
N


ATOM
3646
CA
TYR
A
233
65.204
−12.730
−3.202
1.00
18.66
C


ATOM
3648
CB
TYR
A
233
65.255
−13.628
−1.965
1.00
18.13
C


ATOM
3651
CG
TYR
A
233
64.779
−15.052
−2.158
1.00
16.87
C


ATOM
3652
CD1
TYR
A
233
63.932
−15.407
−3.210
1.00
18.84
C


ATOM
3654
CE1
TYR
A
233
63.505
−16.722
−3.377
1.00
18.16
C


ATOM
3656
CZ
TYR
A
233
63.909
−17.679
−2.475
1.00
16.04
C


ATOM
3657
OH
TYR
A
233
63.491
−18.971
−2.632
1.00
17.36
O


ATOM
3659
CE2
TYR
A
233
64.742
−17.349
−1.419
1.00
15.74
C


ATOM
3661
CD2
TYR
A
233
65.162
−16.048
−1.262
1.00
14.72
C


ATOM
3663
C
TYR
A
233
65.642
−11.326
−2.811
1.00
19.90
C


ATOM
3664
O
TYR
A
233
64.812
−10.444
−2.593
1.00
20.31
O


ATOM
3666
N
GLN
A
234
66.948
−11.117
−2.724
1.00
21.64
N


ATOM
3667
CA
GLN
A
234
67.465
−9.798
−2.405
1.00
22.71
C


ATOM
3669
CB
GLN
A
234
68.988
−9.832
−2.254
1.00
22.62
C


ATOM
3672
CG
GLN
A
234
69.412
−10.450
−0.925
1.00
25.66
C


ATOM
3675
CD
GLN
A
234
70.886
−10.829
−0.847
1.00
29.48
C


ATOM
3676
OE1
GLN
A
234
71.711
−10.388
−1.654
1.00
31.88
O


ATOM
3677
NE2
GLN
A
234
71.222
−11.653
0.144
1.00
26.29
N


ATOM
3680
C
GLN
A
234
67.003
−8.764
−3.434
1.00
23.28
C


ATOM
3681
O
GLN
A
234
66.599
−7.669
−3.054
1.00
23.39
O


ATOM
3683
N
ARG
A
235
67.017
−9.116
−4.720
1.00
24.29
N


ATOM
3684
CA
ARG
A
235
66.543
−8.188
−5.757
1.00
25.70
C


ATOM
3686
CB
ARG
A
235
66.903
−8.673
−7.172
1.00
26.02
C


ATOM
3689
CG
ARG
A
235
66.453
−7.706
−8.277
1.00
31.24
C


ATOM
3692
CD
ARG
A
235
67.168
−7.912
−9.620
1.00
37.34
C


ATOM
3695
NE
ARG
A
235
67.158
−9.314
−10.049
1.00
43.96
N


ATOM
3697
CZ
ARG
A
235
68.235
−10.097
−10.187
1.00
48.83
C


ATOM
3698
NH1
ARG
A
235
69.468
−9.644
−9.955
1.00
49.02
N


ATOM
3701
NH2
ARG
A
235
68.076
−11.361
−10.575
1.00
49.59
N


ATOM
3704
C
ARG
A
235
65.035
−7.948
−5.628
1.00
25.62
C


ATOM
3705
O
ARG
A
235
64.566
−6.817
−5.728
1.00
26.36
O


ATOM
3707
N
ASP
A
236
64.283
−9.013
−5.387
1.00
25.71
N


ATOM
3708
CA
ASP
A
236
62.840
−8.906
−5.197
1.00
26.23
C


ATOM
3710
CB
ASP
A
236
62.229
−10.291
−4.939
1.00
25.67
C


ATOM
3713
CG
ASP
A
236
62.331
−11.222
−6.141
1.00
26.60
C


ATOM
3714
OD1
ASP
A
236
62.449
−10.736
−7.293
1.00
24.55
O


ATOM
3715
OD2
ASP
A
236
62.289
−12.451
−5.925
1.00
24.17
O


ATOM
3716
C
ASP
A
236
62.510
−7.982
−4.023
1.00
26.70
C


ATOM
3717
O
ASP
A
236
61.727
−7.045
−4.159
1.00
26.54
O


ATOM
3719
N
LEU
A
237
63.120
−8.259
−2.875
1.00
27.19
N


ATOM
3720
CA
LEU
A
237
62.841
−7.526
−1.649
1.00
27.53
C


ATOM
3722
CB
LEU
A
237
63.583
−8.172
−0.475
1.00
26.81
C


ATOM
3725
CG
LEU
A
237
63.382
−7.580
0.922
1.00
25.84
C


ATOM
3727
CD1
LEU
A
237
61.918
−7.449
1.268
1.00
23.19
C


ATOM
3731
CD2
LEU
A
237
64.103
−8.424
1.960
1.00
24.06
C


ATOM
3735
C
LEU
A
237
63.207
−6.048
−1.781
1.00
29.70
C


ATOM
3736
O
LEU
A
237
62.472
−5.188
−1.291
1.00
29.95
O


ATOM
3738
N
ARG
A
238
64.328
−5.754
−2.445
1.00
31.65
N


ATOM
3739
CA
ARG
A
238
64.733
−4.363
−2.704
1.00
33.35
C


ATOM
3741
CB
ARG
A
238
66.086
−4.276
−3.436
1.00
33.92
C


ATOM
3744
CG
ARG
A
238
67.321
−4.340
−2.522
1.00
37.39
C


ATOM
3747
CD
ARG
A
238
68.577
−3.791
−3.213
1.00
40.93
C


ATOM
3750
NE
ARG
A
238
68.871
−4.468
−4.484
1.00
44.17
N


ATOM
3752
CZ
ARG
A
238
69.576
−5.596
−4.624
1.00
42.76
C


ATOM
3753
NH1
ARG
A
238
70.095
−6.229
−3.572
1.00
40.79
N


ATOM
3756
NH2
ARG
A
238
69.764
−6.100
−5.841
1.00
40.46
N


ATOM
3759
C
ARG
A
238
63.676
−3.613
−3.509
1.00
33.94
C


ATOM
3760
O
ARG
A
238
63.364
−2.463
−3.197
1.00
35.96
O


ATOM
3762
N
GLU
A
239
63.139
−4.260
−4.539
1.00
33.49
N


ATOM
3763
CA
GLU
A
239
62.105
−3.661
−5.381
1.00
34.02
C


ATOM
3765
CB
GLU
A
239
61.872
−4.534
−6.617
1.00
35.73
C


ATOM
3768
CG
GLU
A
239
60.916
−3.949
−7.657
1.00
43.69
C


ATOM
3771
CD
GLU
A
239
60.865
−4.780
−8.944
1.00
53.52
C


ATOM
3772
OE1
GLU
A
239
61.938
−5.028
−9.542
1.00
56.61
O


ATOM
3773
OE2
GLU
A
239
59.750
−5.176
−9.358
1.00
57.59
O


ATOM
3774
C
GLU
A
239
60.802
−3.494
−4.601
1.00
32.16
C


ATOM
3775
O
GLU
A
239
60.096
−2.499
−4.754
1.00
32.78
O


ATOM
3777
N
THR
A
240
60.495
−4.475
−3.764
1.00
29.87
N


ATOM
3778
CA
THR
A
240
59.300
−4.445
−2.935
1.00
28.58
C


ATOM
3780
CB
THR
A
240
59.005
−5.846
−2.359
1.00
27.73
C


ATOM
3782
OG1
THR
A
240
58.792
−6.757
−3.443
1.00
27.30
O


ATOM
3784
CG2
THR
A
240
57.781
−5.834
−1.476
1.00
24.26
C


ATOM
3788
C
THR
A
240
59.448
−3.414
−1.816
1.00
28.92
C


ATOM
3789
O
THR
A
240
58.492
−2.712
−1.484
1.00
28.92
O


ATOM
3791
N
SER
A
241
60.645
−3.312
−1.246
1.00
29.02
N


ATOM
3792
CA
SER
A
241
60.936
−2.251
−0.280
1.00
29.30
C


ATOM
3794
CB
SER
A
241
62.387
−2.313
0.195
1.00
29.37
C


ATOM
3797
OG
SER
A
241
62.560
−3.360
1.130
1.00
31.18
O


ATOM
3799
C
SER
A
241
60.642
−0.878
−0.865
1.00
29.06
C


ATOM
3800
O
SER
A
241
59.961
−0.083
−0.231
1.00
28.96
O


ATOM
3802
N
ARG
A
242
61.139
−0.611
−2.071
1.00
30.15
N


ATOM
3803
CA
ARG
A
242
60.921
0.690
−2.729
1.00
31.62
C


ATOM
3805
CB
ARG
A
242
61.502
0.721
−4.153
1.00
32.75
C


ATOM
3808
CG
ARG
A
242
62.980
1.119
−4.241
1.00
38.72
C


ATOM
3811
CD
ARG
A
242
63.328
1.686
−5.627
1.00
46.19
C


ATOM
3814
NE
ARG
A
242
62.847
0.824
−6.716
1.00
52.17
N


ATOM
3816
CZ
ARG
A
242
63.543
−0.155
−7.301
1.00
55.43
C


ATOM
3817
NH1
ARG
A
242
62.984
−0.869
−8.276
1.00
55.55
N


ATOM
3820
NH2
ARG
A
242
64.790
−0.435
−6.927
1.00
57.13
N


ATOM
3823
C
ARG
A
242
59.442
1.038
−2.786
1.00
30.15
C


ATOM
3824
O
ARG
A
242
59.043
2.150
−2.434
1.00
30.56
O


ATOM
3826
N
TRP
A
243
58.634
0.084
−3.231
1.00
28.89
N


ATOM
3827
CA
TRP
A
243
57.188
0.267
−3.271
1.00
28.06
C


ATOM
3829
CB
TRP
A
243
56.506
−0.978
−3.861
1.00
27.63
C


ATOM
3832
CG
TRP
A
243
55.039
−1.063
−3.572
1.00
26.01
C


ATOM
3833
CD1
TRP
A
243
54.030
−0.482
−4.281
1.00
28.68
C


ATOM
3835
NE1
TRP
A
243
52.816
−0.782
−3.703
1.00
28.99
N


ATOM
3837
CE2
TRP
A
243
53.030
−1.564
−2.600
1.00
20.27
C


ATOM
3838
CD2
TRP
A
243
54.418
−1.764
−2.485
1.00
19.64
C


ATOM
3839
CE3
TRP
A
243
54.906
−2.529
−1.418
1.00
21.12
C


ATOM
3841
CZ3
TRP
A
243
54.005
−3.069
−0.521
1.00
19.24
C


ATOM
3843
CH2
TRP
A
243
52.626
−2.851
−0.663
1.00
23.00
C


ATOM
3845
CZ2
TRP
A
243
52.123
−2.103
−1.698
1.00
21.52
C


ATOM
3847
C
TRP
A
243
56.660
0.579
−1.865
1.00
27.62
C


ATOM
3848
O
TRP
A
243
55.895
1.520
−1.676
1.00
27.74
O


ATOM
3850
N
TRP
A
244
57.100
−0.204
−0.887
1.00
26.79
N


ATOM
3851
CA
TRP
A
244
56.627
−0.082
0.490
1.00
26.29
C


ATOM
3853
CB
TRP
A
244
57.172
−1.243
1.321
1.00
25.46
C


ATOM
3856
CG
TRP
A
244
56.644
−1.319
2.705
1.00
23.15
C


ATOM
3857
CD1
TRP
A
244
57.369
−1.283
3.861
1.00
23.24
C


ATOM
3859
NE1
TRP
A
244
56.535
−1.384
4.946
1.00
23.78
N


ATOM
3861
CE2
TRP
A
244
55.243
−1.490
4.503
1.00
23.38
C


ATOM
3862
CD2
TRP
A
244
55.272
−1.448
3.096
1.00
24.53
C


ATOM
3863
CE3
TRP
A
244
54.063
−1.529
2.392
1.00
24.59
C


ATOM
3865
CZ3
TRP
A
244
52.887
−1.651
3.101
1.00
20.46
C


ATOM
3867
CH2
TRP
A
244
52.890
−1.697
4.500
1.00
22.43
C


ATOM
3869
CZ2
TRP
A
244
54.053
−1.616
5.219
1.00
24.18
C


ATOM
3871
C
TRP
A
244
57.002
1.249
1.143
1.00
26.77
C


ATOM
3872
O
TRP
A
244
56.183
1.852
1.837
1.00
25.73
O


ATOM
3874
N
ARG
A
245
58.231
1.703
0.933
1.00
28.23
N


ATOM
3875
CA
ARG
A
245
58.668
2.972
1.519
1.00
30.83
C


ATOM
3877
CB
ARG
A
245
60.194
3.145
1.433
1.00
31.39
C


ATOM
3880
CG
ARG
A
245
60.911
2.560
2.660
1.00
37.50
C


ATOM
3883
CD
ARG
A
245
62.411
2.813
2.666
1.00
44.44
C


ATOM
3886
NE
ARG
A
245
63.130
1.800
1.893
1.00
49.18
N


ATOM
3888
CZ
ARG
A
245
63.510
1.926
0.621
1.00
53.23
C


ATOM
3889
NH1
ARG
A
245
64.149
0.922
0.032
1.00
54.44
N


ATOM
3892
NH2
ARG
A
245
63.260
3.036
−0.074
1.00
54.41
N


ATOM
3895
C
ARG
A
245
57.927
4.155
0.902
1.00
31.00
C


ATOM
3896
O
ARG
A
245
57.562
5.096
1.603
1.00
30.87
O


ATOM
3898
N
ARG
A
246
57.672
4.084
−0.399
1.00
31.87
N


ATOM
3899
CA
ARG
A
246
56.943
5.140
−1.091
1.00
32.47
C


ATOM
3901
CB
ARG
A
246
57.126
5.026
−2.608
1.00
34.15
C


ATOM
3904
CG
ARG
A
246
58.396
5.733
−3.103
1.00
42.54
C


ATOM
3907
CD
ARG
A
246
58.820
5.323
−4.516
1.00
52.53
C


ATOM
3910
NE
ARG
A
246
57.752
5.471
−5.508
1.00
61.41
N


ATOM
3912
CZ
ARG
A
246
57.911
5.319
−6.824
1.00
68.27
C


ATOM
3913
NH1
ARG
A
246
59.105
5.028
−7.339
1.00
71.09
N


ATOM
3916
NH2
ARG
A
246
56.868
5.472
−7.638
1.00
69.80
N


ATOM
3919
C
ARG
A
246
55.465
5.190
−0.710
1.00
30.26
C


ATOM
3920
O
ARG
A
246
54.894
6.275
−0.598
1.00
32.45
O


ATOM
3922
N
VAL
A
247
54.847
4.037
−0.494
1.00
27.09
N


ATOM
3923
CA
VAL
A
247
53.481
4.013
0.040
1.00
25.76
C


ATOM
3925
CB
VAL
A
247
52.904
2.581
0.070
1.00
25.78
C


ATOM
3927
CG1
VAL
A
247
51.497
2.566
0.660
1.00
23.89
C


ATOM
3931
CG2
VAL
A
247
52.892
1.988
−1.330
1.00
24.93
C


ATOM
3935
C
VAL
A
247
53.456
4.640
1.446
1.00
25.37
C


ATOM
3936
O
VAL
A
247
52.497
5.309
1.809
1.00
25.32
O


ATOM
3938
N
GLY
A
248
54.520
4.414
2.219
1.00
24.97
N


ATOM
3939
CA
GLY
A
248
54.779
5.135
3.468
1.00
23.78
C


ATOM
3942
C
GLY
A
248
53.741
4.971
4.559
1.00
23.10
C


ATOM
3943
O
GLY
A
248
53.625
5.806
5.447
1.00
22.20
O


ATOM
3945
N
LEU
A
249
53.004
3.877
4.516
1.00
24.18
N


ATOM
3946
CA
LEU
A
249
51.839
3.723
5.377
1.00
25.74
C


ATOM
3948
CB
LEU
A
249
50.958
2.584
4.857
1.00
25.16
C


ATOM
3951
CG
LEU
A
249
49.458
2.750
5.072
1.00
25.16
C


ATOM
3953
CD1
LEU
A
249
48.930
4.030
4.424
1.00
23.83
C


ATOM
3957
CD2
LEU
A
249
48.746
1.521
4.519
1.00
23.25
C


ATOM
3961
C
LEU
A
249
52.219
3.493
6.845
1.00
27.35
C


ATOM
3962
O
LEU
A
249
51.562
4.024
7.747
1.00
27.47
O


ATOM
3964
N
ALA
A
250
53.277
2.714
7.079
1.00
28.70
N


ATOM
3965
CA
ALA
A
250
53.732
2.415
8.442
1.00
29.93
C


ATOM
3967
CB
ALA
A
250
54.728
1.273
8.437
1.00
30.24
C


ATOM
3971
C
ALA
A
250
54.340
3.645
9.101
1.00
30.67
C


ATOM
3972
O
ALA
A
250
54.256
3.814
10.320
1.00
31.97
O


ATOM
3974
N
THR
A
251
54.949
4.500
8.286
1.00
30.88
N


ATOM
3975
CA
THR
A
251
55.484
5.773
8.754
1.00
30.79
C


ATOM
3977
CB
THR
A
251
56.355
6.437
7.674
1.00
30.36
C


ATOM
3979
OG1
THR
A
251
57.329
5.498
7.205
1.00
26.92
O


ATOM
3981
CG2
THR
A
251
57.057
7.667
8.235
1.00
29.77
C


ATOM
3985
C
THR
A
251
54.391
6.766
9.161
1.00
31.81
C


ATOM
3986
O
THR
A
251
54.578
7.524
10.111
1.00
34.30
O


ATOM
3988
N
LYS
A
252
53.263
6.770
8.452
1.00
31.78
N


ATOM
3989
CA
LYS
A
252
52.210
7.770
8.686
1.00
32.29
C


ATOM
3991
CB
LYS
A
252
51.529
8.170
7.369
1.00
32.78
C


ATOM
3994
CG
LYS
A
252
52.459
8.823
6.338
1.00
35.10
C


ATOM
3997
CD
LYS
A
252
52.882
10.238
6.730
1.00
39.23
C


ATOM
4000
CE
LYS
A
252
53.945
10.775
5.776
1.00
41.74
C


ATOM
4003
NZ
LYS
A
252
54.463
12.107
6.183
1.00
42.94
N


ATOM
4007
C
LYS
A
252
51.165
7.309
9.703
1.00
31.68
C


ATOM
4008
O
LYS
A
252
50.545
8.134
10.367
1.00
31.90
O


ATOM
4010
N
LEU
A
253
50.962
6.001
9.814
1.00
31.58
N


ATOM
4011
CA
LEU
A
253
50.116
5.449
10.866
1.00
31.41
C


ATOM
4013
CB
LEU
A
253
49.391
4.201
10.370
1.00
30.57
C


ATOM
4016
CG
LEU
A
253
48.566
4.376
9.096
1.00
31.21
C


ATOM
4018
CD1
LEU
A
253
47.842
3.089
8.773
1.00
31.56
C


ATOM
4022
CD2
LEU
A
253
47.580
5.521
9.212
1.00
32.60
C


ATOM
4026
C
LEU
A
253
50.981
5.118
12.080
1.00
31.78
C


ATOM
4027
O
LEU
A
253
51.865
4.260
12.006
1.00
32.06
O


ATOM
4029
N
HIS
A
254
50.732
5.806
13.192
1.00
32.11
N


ATOM
4030
CA
HIS
A
254
51.542
5.629
14.405
1.00
33.05
C


ATOM
4032
CB
HIS
A
254
51.271
6.750
15.430
1.00
34.30
C


ATOM
4035
CG
HIS
A
254
49.898
6.713
16.042
1.00
41.82
C


ATOM
4036
ND1
HIS
A
254
48.794
7.278
15.437
1.00
46.79
N


ATOM
4038
CE1
HIS
A
254
47.733
7.104
16.206
1.00
48.23
C


ATOM
4040
NE2
HIS
A
254
48.108
6.452
17.293
1.00
46.70
N


ATOM
4042
CD2
HIS
A
254
49.457
6.196
17.216
1.00
46.49
C


ATOM
4044
C
HIS
A
254
51.350
4.254
15.044
1.00
31.06
C


ATOM
4045
O
HIS
A
254
52.284
3.703
15.610
1.00
30.95
O


ATOM
4047
N
PHE
A
255
50.145
3.707
14.932
1.00
30.16
N


ATOM
4048
CA
PHE
A
255
49.803
2.405
15.520
1.00
29.65
C


ATOM
4050
CB
PHE
A
255
48.288
2.308
15.738
1.00
29.40
C


ATOM
4053
CG
PHE
A
255
47.501
2.234
14.462
1.00
28.96
C


ATOM
4054
CD1
PHE
A
255
47.088
3.390
13.816
1.00
29.52
C


ATOM
4056
CE1
PHE
A
255
46.380
3.325
12.631
1.00
27.79
C


ATOM
4058
CZ
PHE
A
255
46.074
2.099
12.079
1.00
27.35
C


ATOM
4060
CE2
PHE
A
255
46.478
0.943
12.710
1.00
28.82
C


ATOM
4062
CD2
PHE
A
255
47.189
1.011
13.896
1.00
27.34
C


ATOM
4064
C
PHE
A
255
50.244
1.220
14.661
1.00
29.59
C


ATOM
4065
O
PHE
A
255
50.298
0.088
15.139
1.00
29.60
O


ATOM
4067
N
ALA
A
256
50.543
1.479
13.390
1.00
29.88
N


ATOM
4068
CA
ALA
A
256
50.767
0.410
12.418
1.00
29.61
C


ATOM
4070
CB
ALA
A
256
50.745
0.980
11.015
1.00
29.60
C


ATOM
4074
C
ALA
A
256
52.068
−0.344
12.647
1.00
29.65
C


ATOM
4075
O
ALA
A
256
53.076
0.243
13.039
1.00
29.60
O


ATOM
4077
N
ARG
A
257
52.035
−1.649
12.387
1.00
30.36
N


ATOM
4078
CA
ARG
A
257
53.240
−2.478
12.385
1.00
30.24
C


ATOM
4080
CB
ARG
A
257
52.924
−3.904
12.839
1.00
29.56
C


ATOM
4083
CG
ARG
A
257
52.496
−4.014
14.286
1.00
31.34
C


ATOM
4086
CD
ARG
A
257
52.158
−5.450
14.640
1.00
33.32
C


ATOM
4089
NE
ARG
A
257
50.984
−5.926
13.912
1.00
34.21
N


ATOM
4091
CZ
ARG
A
257
50.711
−7.204
13.657
1.00
40.51
C


ATOM
4092
NH1
ARG
A
257
51.533
−8.168
14.063
1.00
44.76
N


ATOM
4095
NH2
ARG
A
257
49.611
−7.525
12.977
1.00
42.49
N


ATOM
4098
C
ARG
A
257
53.845
−2.526
10.982
1.00
30.05
C


ATOM
4099
O
ARG
A
257
53.130
−2.759
10.004
1.00
28.85
O


ATOM
4101
N
ASP
A
258
55.158
−2.290
10.903
1.00
30.41
N


ATOM
4102
CA
ASP
A
258
55.934
−2.486
9.679
1.00
30.29
C


ATOM
4104
CB
ASP
A
258
57.100
−1.486
9.616
1.00
30.46
C


ATOM
4107
CG
ASP
A
258
57.892
−1.558
8.301
1.00
30.96
C


ATOM
4108
OD1
ASP
A
258
57.613
−2.420
7.447
1.00
29.66
O


ATOM
4109
OD2
ASP
A
258
58.811
−0.737
8.119
1.00
34.44
O


ATOM
4110
C
ASP
A
258
56.453
−3.929
9.670
1.00
30.30
C


ATOM
4111
O
ASP
A
258
57.355
−4.284
10.441
1.00
29.85
O


ATOM
4113
N
ARG
A
259
55.874
−4.756
8.803
1.00
29.36
N


ATOM
4114
CA
ARG
A
259
56.272
−6.147
8.706
1.00
30.06
C


ATOM
4116
CB
ARG
A
259
55.242
−7.029
9.423
1.00
31.33
C


ATOM
4119
CG
ARG
A
259
55.229
−6.847
10.938
1.00
35.62
C


ATOM
4122
CD
ARG
A
259
54.305
−7.840
11.634
1.00
43.50
C


ATOM
4125
NE
ARG
A
259
54.908
−9.166
11.805
1.00
49.86
N


ATOM
4127
CZ
ARG
A
259
55.756
−9.506
12.779
1.00
53.74
C


ATOM
4128
NH1
ARG
A
259
56.142
−8.623
13.702
1.00
55.51
N


ATOM
4131
NH2
ARG
A
259
56.229
−10.746
12.827
1.00
54.56
N


ATOM
4134
C
ARG
A
259
56.463
−6.567
7.244
1.00
29.15
C


ATOM
4135
O
ARG
A
259
55.895
−7.565
6.793
1.00
29.19
O


ATOM
4137
N
LEU
A
260
57.272
−5.804
6.511
1.00
27.50
N


ATOM
4138
CA
LEU
A
260
57.531
−6.109
5.109
1.00
26.23
C


ATOM
4140
CB
LEU
A
260
58.064
−4.889
4.346
1.00
25.40
C


ATOM
4143
CG
LEU
A
260
58.363
−5.176
2.865
1.00
24.10
C


ATOM
4145
CD1
LEU
A
260
57.111
−5.665
2.120
1.00
17.33
C


ATOM
4149
CD2
LEU
A
260
58.937
−3.969
2.188
1.00
24.52
C


ATOM
4153
C
LEU
A
260
58.521
−7.257
4.979
1.00
25.74
C


ATOM
4154
O
LEU
A
260
58.303
−8.181
4.193
1.00
26.84
O


ATOM
4156
N
ILE
A
261
59.611
−7.187
5.733
1.00
23.99
N


ATOM
4157
CA
ILE
A
261
60.636
−8.219
5.673
1.00
23.51
C


ATOM
4159
CB
ILE
A
261
61.860
−7.867
6.561
1.00
23.42
C


ATOM
4161
CG1
ILE
A
261
62.589
−6.629
6.001
1.00
26.27
C


ATOM
4164
CD1
ILE
A
261
63.300
−5.770
7.055
1.00
22.43
C


ATOM
4168
CG2
ILE
A
261
62.811
−9.043
6.643
1.00
18.52
C


ATOM
4172
C
ILE
A
261
60.047
−9.577
6.077
1.00
22.92
C


ATOM
4173
O
ILE
A
261
60.236
−10.577
5.380
1.00
21.46
O


ATOM
4175
N
GLU
A
262
59.321
−9.599
7.189
1.00
22.77
N


ATOM
4176
CA
GLU
A
262
58.728
−10.834
7.680
1.00
23.47
C


ATOM
4178
CB
GLU
A
262
58.051
−10.637
9.038
1.00
24.41
C


ATOM
4181
CG
GLU
A
262
59.003
−10.303
10.182
1.00
28.29
C


ATOM
4184
CD
GLU
A
262
59.126
−8.804
10.450
1.00
35.49
C


ATOM
4185
OE1
GLU
A
262
59.085
−7.998
9.487
1.00
34.06
O


ATOM
4186
OE2
GLU
A
262
59.272
−8.439
11.639
1.00
41.58
O


ATOM
4187
C
GLU
A
262
57.711
−11.356
6.677
1.00
23.14
C


ATOM
4188
O
GLU
A
262
57.590
−12.568
6.486
1.00
22.49
O


ATOM
4190
N
SER
A
263
56.991
−10.439
6.034
1.00
22.16
N


ATOM
4191
CA
SER
A
263
56.000
−10.819
5.035
1.00
21.96
C


ATOM
4193
CB
SER
A
263
55.117
−9.631
4.634
1.00
22.71
C


ATOM
4196
OG
SER
A
263
54.192
−9.324
5.672
1.00
24.34
O


ATOM
4198
C
SER
A
263
56.678
−11.415
3.817
1.00
20.76
C


ATOM
4199
O
SER
A
263
56.148
−12.332
3.199
1.00
18.64
O


ATOM
4201
N
PHE
A
264
57.855
−10.904
3.475
1.00
20.69
N


ATOM
4202
CA
PHE
A
264
58.581
−11.438
2.332
1.00
20.32
C


ATOM
4204
CB
PHE
A
264
59.670
−10.485
1.862
1.00
19.12
C


ATOM
4207
CG
PHE
A
264
60.233
−10.873
0.547
1.00
17.93
C


ATOM
4208
CD1
PHE
A
264
59.613
−10.479
−0.618
1.00
16.80
C


ATOM
4210
CE1
PHE
A
264
60.101
−10.876
−1.837
1.00
16.86
C


ATOM
4212
CZ
PHE
A
264
61.226
−11.681
−1.900
1.00
20.16
C


ATOM
4214
CE2
PHE
A
264
61.849
−12.089
−0.744
1.00
17.10
C


ATOM
4216
CD2
PHE
A
264
61.343
−11.699
0.472
1.00
19.87
C


ATOM
4218
C
PHE
A
264
59.177
−12.816
2.636
1.00
21.04
C


ATOM
4219
O
PHE
A
264
59.137
−13.714
1.793
1.00
20.17
O


ATOM
4221
N
TYR
A
265
59.736
−12.962
3.836
1.00
21.97
N


ATOM
4222
CA
TYR
A
265
60.197
−14.256
4.341
1.00
22.83
C


ATOM
4224
CB
TYR
A
265
60.674
−14.108
5.790
1.00
23.02
C


ATOM
4227
CG
TYR
A
265
60.829
−15.397
6.566
1.00
29.40
C


ATOM
4228
CD1
TYR
A
265
61.998
−16.148
6.484
1.00
34.40
C


ATOM
4230
CE1
TYR
A
265
62.146
−17.326
7.214
1.00
37.24
C


ATOM
4232
CZ
TYR
A
265
61.120
−17.759
8.042
1.00
37.62
C


ATOM
4233
OH
TYR
A
265
61.266
−18.924
8.761
1.00
42.46
O


ATOM
4235
CE2
TYR
A
265
59.953
−17.024
8.150
1.00
35.20
C


ATOM
4237
CD2
TYR
A
265
59.815
−15.849
7.419
1.00
34.49
C


ATOM
4239
C
TYR
A
265
59.078
−15.285
4.247
1.00
22.14
C


ATOM
4240
O
TYR
A
265
59.304
−16.413
3.828
1.00
23.08
O


ATOM
4242
N
TRP
A
266
57.876
−14.881
4.642
1.00
21.35
N


ATOM
4243
CA
TRP
A
266
56.679
−15.712
4.511
1.00
20.58
C


ATOM
4245
CB
TRP
A
266
55.456
−14.941
5.028
1.00
20.00
C


ATOM
4248
CG
TRP
A
266
54.150
−15.640
4.901
1.00
20.69
C


ATOM
4249
CD1
TRP
A
266
53.306
−15.618
3.826
1.00
21.65
C


ATOM
4251
NE1
TRP
A
266
52.183
−16.371
4.087
1.00
20.12
N


ATOM
4253
CE2
TRP
A
266
52.284
−16.885
5.352
1.00
22.19
C


ATOM
4254
CD2
TRP
A
266
53.507
−16.440
5.896
1.00
21.10
C


ATOM
4255
CE3
TRP
A
266
53.844
−16.820
7.195
1.00
21.72
C


ATOM
4257
CZ3
TRP
A
266
52.973
−17.635
7.895
1.00
25.91
C


ATOM
4259
CH2
TRP
A
266
51.768
−18.067
7.327
1.00
24.55
C


ATOM
4261
CZ2
TRP
A
266
51.406
−17.701
6.061
1.00
25.05
C


ATOM
4263
C
TRP
A
266
56.486
−16.145
3.054
1.00
20.13
C


ATOM
4264
O
TRP
A
266
56.375
−17.336
2.768
1.00
21.08
O


ATOM
4266
N
ALA
A
267
56.480
−15.182
2.137
1.00
18.67
N


ATOM
4267
CA
ALA
A
267
56.264
−15.472
0.719
1.00
18.28
C


ATOM
4269
CB
ALA
A
267
56.228
−14.187
−0.091
1.00
17.55
C


ATOM
4273
C
ALA
A
267
57.304
−16.437
0.139
1.00
17.99
C


ATOM
4274
O
ALA
A
267
56.968
−17.253
−0.711
1.00
19.18
O


ATOM
4276
N
VAL
A
268
58.549
−16.354
0.605
1.00
17.57
N


ATOM
4277
CA
VAL
A
268
59.601
−17.300
0.192
1.00
16.55
C


ATOM
4279
CB
VAL
A
268
60.972
−16.948
0.824
1.00
15.16
C


ATOM
4281
CG1
VAL
A
268
61.953
−18.080
0.659
1.00
14.00
C


ATOM
4285
CG2
VAL
A
268
61.532
−15.696
0.190
1.00
12.76
C


ATOM
4289
C
VAL
A
268
59.208
−18.745
0.517
1.00
16.99
C


ATOM
4290
O
VAL
A
268
59.478
−19.663
−0.257
1.00
17.36
O


ATOM
4292
N
GLY
A
269
58.551
−18.934
1.654
1.00
17.99
N


ATOM
4293
CA
GLY
A
269
57.964
−20.225
1.993
1.00
18.12
C


ATOM
4296
C
GLY
A
269
56.865
−20.692
1.051
1.00
17.71
C


ATOM
4297
O
GLY
A
269
56.739
−21.893
0.810
1.00
20.02
O


ATOM
4299
N
VAL
A
270
56.068
−19.757
0.532
1.00
15.99
N


ATOM
4300
CA
VAL
A
270
54.952
−20.092
−0.358
1.00
16.46
C


ATOM
4302
CB
VAL
A
270
53.895
−18.959
−0.401
1.00
16.76
C


ATOM
4304
CG1
VAL
A
270
52.904
−19.176
−1.540
1.00
14.34
C


ATOM
4308
CG2
VAL
A
270
53.168
−18.863
0.931
1.00
15.01
C


ATOM
4312
C
VAL
A
270
55.403
−20.409
−1.790
1.00
17.14
C


ATOM
4313
O
VAL
A
270
54.893
−21.350
−2.403
1.00
17.58
O


ATOM
4315
N
ALA
A
271
56.344
−19.622
−2.318
1.00
16.61
N


ATOM
4316
CA
ALA
A
271
56.869
−19.828
−3.672
1.00
16.49
C


ATOM
4318
CB
ALA
A
271
56.105
−18.989
−4.669
1.00
15.65
C


ATOM
4322
C
ALA
A
271
58.362
−19.495
−3.729
1.00
18.32
C


ATOM
4323
O
ALA
A
271
58.753
−18.336
−3.920
1.00
18.32
O


ATOM
4325
N
PHE
A
272
59.196
−20.521
−3.580
1.00
18.87
N


ATOM
4326
CA
PHE
A
272
60.628
−20.318
−3.446
1.00
18.58
C


ATOM
4328
CB
PHE
A
272
61.246
−21.406
−2.581
1.00
18.99
C


ATOM
4331
CG
PHE
A
272
61.436
−22.698
−3.291
1.00
21.53
C


ATOM
4332
CD1
PHE
A
272
62.586
−22.942
−4.013
1.00
24.73
C


ATOM
4334
CE1
PHE
A
272
62.753
−24.140
−4.677
1.00
25.53
C


ATOM
4336
CZ
PHE
A
272
61.768
−25.098
−4.626
1.00
23.65
C


ATOM
4338
CE2
PHE
A
272
60.625
−24.867
−3.916
1.00
22.26
C


ATOM
4340
CD2
PHE
A
272
60.459
−23.675
−3.247
1.00
24.18
C


ATOM
4342
C
PHE
A
272
61.353
−20.257
−4.774
1.00
19.15
C


ATOM
4343
O
PHE
A
272
62.367
−19.580
−4.873
1.00
19.58
O


ATOM
4345
N
GLU
A
273
60.849
−20.951
−5.793
1.00
19.97
N


ATOM
4346
CA
GLU
A
273
61.595
−21.081
−7.048
1.00
20.05
C


ATOM
4348
CB
GLU
A
273
60.874
−21.975
−8.063
1.00
20.71
C


ATOM
4351
CG
GLU
A
273
60.715
−23.430
−7.648
1.00
23.15
C


ATOM
4354
CD
GLU
A
273
59.417
−23.709
−6.916
1.00
28.01
C


ATOM
4355
OE1
GLU
A
273
58.852
−22.767
−6.327
1.00
32.28
O


ATOM
4356
OE2
GLU
A
273
58.958
−24.875
−6.933
1.00
34.22
O


ATOM
4357
C
GLU
A
273
61.858
−19.709
−7.665
1.00
19.88
C


ATOM
4358
O
GLU
A
273
60.973
−18.852
−7.665
1.00
20.82
O


ATOM
4360
N
PRO
A
274
63.069
−19.503
−8.213
1.00
19.39
N


ATOM
4361
CA
PRO
A
274
63.489
−18.180
−8.653
1.00
19.28
C


ATOM
4363
CB
PRO
A
274
64.836
−18.445
−9.336
1.00
19.79
C


ATOM
4366
CG
PRO
A
274
64.845
−19.885
−9.629
1.00
19.06
C


ATOM
4369
CD
PRO
A
274
64.069
−20.527
−8.555
1.00
19.21
C


ATOM
4372
C
PRO
A
274
62.512
−17.504
−9.609
1.00
19.22
C


ATOM
4373
O
PRO
A
274
62.280
−16.305
−9.489
1.00
20.35
O


ATOM
4374
N
GLN
A
275
61.928
−18.262
−10.532
1.00
19.49
N


ATOM
4375
CA
GLN
A
275
60.957
−17.691
−11.475
1.00
19.56
C


ATOM
4377
CB
GLN
A
275
60.544
−18.713
−12.539
1.00
20.27
C


ATOM
4380
CG
GLN
A
275
59.793
−19.952
−12.035
1.00
21.82
C


ATOM
4383
CD
GLN
A
275
60.719
−21.085
−11.619
1.00
27.78
C


ATOM
4384
OE1
GLN
A
275
61.861
−20.854
−11.201
1.00
26.35
O


ATOM
4385
NE2
GLN
A
275
60.224
−22.324
−11.723
1.00
30.59
N


ATOM
4388
C
GLN
A
275
59.700
−17.083
−10.837
1.00
19.47
C


ATOM
4389
O
GLN
A
275
59.027
−16.293
−11.481
1.00
18.56
O


ATOM
4391
N
TYR
A
276
59.379
−17.433
−9.588
1.00
20.01
N


ATOM
4392
CA
TYR
A
276
58.154
−16.910
−8.954
1.00
20.87
C


ATOM
4394
CB
TYR
A
276
57.511
−17.974
−8.045
1.00
21.44
C


ATOM
4397
CG
TYR
A
276
57.110
−19.235
−8.779
1.00
22.50
C


ATOM
4398
CD1
TYR
A
276
56.284
−19.182
−9.898
1.00
19.93
C


ATOM
4400
CE1
TYR
A
276
55.919
−20.335
−10.572
1.00
21.58
C


ATOM
4402
CZ
TYR
A
276
56.380
−21.562
−10.130
1.00
25.30
C


ATOM
4403
OH
TYR
A
276
56.017
−22.708
−10.794
1.00
30.57
O


ATOM
4405
CE2
TYR
A
276
57.200
−21.645
−9.022
1.00
23.76
C


ATOM
4407
CD2
TYR
A
276
57.555
−20.482
−8.350
1.00
24.50
C


ATOM
4409
C
TYR
A
276
58.344
−15.583
−8.193
1.00
20.15
C


ATOM
4410
O
TYR
A
276
57.736
−15.371
−7.149
1.00
20.74
O


ATOM
4412
N
SER
A
277
59.156
−14.681
−8.731
1.00
19.69
N


ATOM
4413
CA
SER
A
277
59.308
−13.353
−8.141
1.00
19.79
C


ATOM
4415
CB
SER
A
277
60.273
−12.494
−8.961
1.00
19.15
C


ATOM
4418
OG
SER
A
277
61.606
−12.879
−8.708
1.00
18.79
O


ATOM
4420
C
SER
A
277
57.970
−12.629
−7.992
1.00
19.59
C


ATOM
4421
O
SER
A
277
57.685
−12.061
−6.939
1.00
20.41
O


ATOM
4423
N
ASP
A
278
57.152
−12.648
−9.038
1.00
19.45
N


ATOM
4424
CA
ASP
A
278
55.851
−11.990
−8.980
1.00
20.09
C


ATOM
4426
CB
ASP
A
278
55.105
−12.115
−10.311
1.00
20.84
C


ATOM
4429
CG
ASP
A
278
55.628
−11.168
−11.368
1.00
22.85
C


ATOM
4430
OD1
ASP
A
278
56.248
−10.146
−11.014
1.00
33.08
O


ATOM
4431
OD2
ASP
A
278
55.407
−11.439
−12.561
1.00
28.05
O


ATOM
4432
C
ASP
A
278
54.984
−12.538
−7.850
1.00
19.48
C


ATOM
4433
O
ASP
A
278
54.317
−11.777
−7.166
1.00
19.06
O


ATOM
4435
N
CYS
A
279
54.997
−13.851
−7.652
1.00
18.86
N


ATOM
4436
CA
CYS
A
279
54.188
−14.455
−6.608
1.00
19.06
C


ATOM
4438
CB
CYS
A
279
54.171
−15.977
−6.746
1.00
20.08
C


ATOM
4441
SG
CYS
A
279
53.085
−16.784
−5.541
1.00
19.71
S


ATOM
4443
C
CYS
A
279
54.692
−14.071
−5.224
1.00
19.24
C


ATOM
4444
O
CYS
A
279
53.901
−13.766
−4.323
1.00
19.67
O


ATOM
4446
N
ARG
A
280
56.008
−14.096
−5.048
1.00
18.79
N


ATOM
4447
CA
ARG
A
280
56.593
−13.653
−3.793
1.00
18.97
C


ATOM
4449
CB
ARG
A
280
58.112
−13.786
−3.829
1.00
19.49
C


ATOM
4452
CG
ARG
A
280
58.617
−15.207
−3.693
1.00
18.21
C


ATOM
4455
CD
ARG
A
280
60.143
−15.230
−3.494
1.00
17.68
C


ATOM
4458
NE
ARG
A
280
60.868
−14.866
−4.710
1.00
14.15
N


ATOM
4460
CZ
ARG
A
280
61.018
−15.657
−5.769
1.00
15.41
C


ATOM
4461
NH1
ARG
A
280
60.495
−16.878
−5.786
1.00
19.91
N


ATOM
4464
NH2
ARG
A
280
61.684
−15.221
−6.827
1.00
18.53
N


ATOM
4467
C
ARG
A
280
56.205
−12.206
−3.510
1.00
19.13
C


ATOM
4468
O
ARG
A
280
55.772
−11.869
−2.417
1.00
19.04
O


ATOM
4470
N
ASN
A
281
56.348
−11.354
−4.514
1.00
20.54
N


ATOM
4471
CA
ASN
A
281
56.056
−9.939
−4.349
1.00
21.33
C


ATOM
4473
CB
ASN
A
281
56.518
−9.167
−5.580
1.00
21.84
C


ATOM
4476
CG
ASN
A
281
58.031
−9.117
−5.694
1.00
24.61
C


ATOM
4477
OD1
ASN
A
281
58.742
−9.247
−4.697
1.00
27.33
O


ATOM
4478
ND2
ASN
A
281
58.530
−8.933
−6.909
1.00
24.11
N


ATOM
4481
C
ASN
A
281
54.587
−9.657
−4.056
1.00
21.18
C


ATOM
4482
O
ASN
A
281
54.265
−8.831
−3.204
1.00
21.72
O


ATOM
4484
N
SER
A
282
53.689
−10.363
−4.724
1.00
20.74
N


ATOM
4485
CA
SER
A
282
52.282
−10.050
−4.563
1.00
21.09
C


ATOM
4487
CB
SER
A
282
51.469
−10.450
−5.808
1.00
20.40
C


ATOM
4490
OG
SER
A
282
50.469
−11.383
−5.509
1.00
26.21
O


ATOM
4492
C
SER
A
282
51.743
−10.620
−3.233
1.00
20.92
C


ATOM
4493
O
SER
A
282
50.850
−10.026
−2.626
1.00
21.89
O


ATOM
4495
N
VAL
A
283
52.318
−11.726
−2.755
1.00
19.67
N


ATOM
4496
CA
VAL
A
283
51.992
−12.254
−1.420
1.00
17.46
C


ATOM
4498
CB
VAL
A
283
52.470
−13.725
−1.265
1.00
17.08
C


ATOM
4500
CG1
VAL
A
283
52.247
−14.238
0.158
1.00
15.97
C


ATOM
4504
CG2
VAL
A
283
51.753
−14.616
−2.241
1.00
12.54
C


ATOM
4508
C
VAL
A
283
52.585
−11.372
−0.302
1.00
17.57
C


ATOM
4509
O
VAL
A
283
51.959
−11.168
0.741
1.00
17.42
O


ATOM
4511
N
ALA
A
284
53.787
−10.846
−0.528
1.00
18.06
N


ATOM
4512
CA
ALA
A
284
54.451
−9.967
0.450
1.00
18.13
C


ATOM
4514
CB
ALA
A
284
55.874
−9.678
0.025
1.00
16.24
C


ATOM
4518
C
ALA
A
284
53.681
−8.660
0.642
1.00
18.68
C


ATOM
4519
O
ALA
A
284
53.438
−8.241
1.766
1.00
20.09
O


ATOM
4521
N
LYS
A
285
53.288
−8.029
−0.459
1.00
19.10
N


ATOM
4522
CA
LYS
A
285
52.470
−6.823
−0.397
1.00
19.09
C


ATOM
4524
CB
LYS
A
285
52.195
−6.274
−1.803
1.00
19.20
C


ATOM
4527
CG
LYS
A
285
53.430
−5.805
−2.569
1.00
21.42
C


ATOM
4530
CD
LYS
A
285
53.113
−5.515
−4.041
1.00
25.96
C


ATOM
4533
CE
LYS
A
285
54.358
−5.051
−4.793
1.00
30.90
C


ATOM
4536
NZ
LYS
A
285
54.070
−4.041
−5.866
1.00
34.43
N


ATOM
4540
C
LYS
A
285
51.142
−7.110
0.316
1.00
18.92
C


ATOM
4541
O
LYS
A
285
50.758
−6.379
1.227
1.00
19.78
O


ATOM
4543
N
MET
A
286
50.446
−8.171
−0.085
1.00
17.63
N


ATOM
4544
CA
MET
A
286
49.159
−8.478
0.519
1.00
17.41
C


ATOM
4546
CB
MET
A
286
48.492
−9.700
−0.132
1.00
17.52
C


ATOM
4549
CG
MET
A
286
47.910
−9.484
−1.539
1.00
16.52
C


ATOM
4552
SD
MET
A
286
47.112
−7.888
−1.833
1.00
19.64
S


ATOM
4553
CE
MET
A
286
48.523
−6.929
−2.405
1.00
14.62
C


ATOM
4557
C
MET
A
286
49.318
−8.717
2.011
1.00
18.00
C


ATOM
4558
O
MET
A
286
48.550
−8.186
2.819
1.00
19.27
O


ATOM
4560
N
PHE
A
287
50.317
−9.499
2.397
1.00
18.02
N


ATOM
4561
CA
PHE
A
287
50.474
−9.812
3.818
1.00
18.38
C


ATOM
4563
CB
PHE
A
287
51.476
−10.960
4.032
1.00
19.21
C


ATOM
4566
CG
PHE
A
287
51.220
−11.788
5.275
1.00
24.14
C


ATOM
4567
CD1
PHE
A
287
52.181
−12.681
5.721
1.00
30.66
C


ATOM
4569
CE1
PHE
A
287
51.965
−13.454
6.861
1.00
33.02
C


ATOM
4571
CZ
PHE
A
287
50.777
−13.330
7.577
1.00
31.77
C


ATOM
4573
CE2
PHE
A
287
49.817
−12.444
7.151
1.00
29.66
C


ATOM
4575
CD2
PHE
A
287
50.035
−11.677
6.006
1.00
28.16
C


ATOM
4577
C
PHE
A
287
50.865
−8.569
4.611
1.00
16.94
C


ATOM
4578
O
PHE
A
287
50.506
−8.440
5.774
1.00
14.32
O


ATOM
4580
N
SER
A
288
51.578
−7.646
3.964
1.00
17.30
N


ATOM
4581
CA
SER
A
288
51.918
−6.364
4.576
1.00
17.86
C


ATOM
4583
CB
SER
A
288
52.901
−5.598
3.705
1.00
18.10
C


ATOM
4586
OG
SER
A
288
54.074
−6.356
3.501
1.00
22.26
O


ATOM
4588
C
SER
A
288
50.683
−5.509
4.836
1.00
17.60
C


ATOM
4589
O
SER
A
288
50.596
−4.866
5.883
1.00
17.11
O


ATOM
4591
N
PHE
A
289
49.734
−5.510
3.896
1.00
17.48
N


ATOM
4592
CA
PHE
A
289
48.457
−4.796
4.083
1.00
17.47
C


ATOM
4594
CB
PHE
A
289
47.658
−4.677
2.777
1.00
17.93
C


ATOM
4597
CG
PHE
A
289
48.041
−3.490
1.955
1.00
19.51
C


ATOM
4598
CD1
PHE
A
289
47.715
−2.208
2.379
1.00
19.83
C


ATOM
4600
CE1
PHE
A
289
48.092
−1.093
1.635
1.00
21.90
C


ATOM
4602
CZ
PHE
A
289
48.800
−1.258
0.452
1.00
22.70
C


ATOM
4604
CE2
PHE
A
289
49.137
−2.536
0.023
1.00
23.24
C


ATOM
4606
CD2
PHE
A
289
48.758
−3.644
0.776
1.00
23.63
C


ATOM
4608
C
PHE
A
289
47.600
−5.460
5.135
1.00
17.18
C


ATOM
4609
O
PHE
A
289
47.081
−4.782
6.018
1.00
16.93
O


ATOM
4611
N
VAL
A
290
47.461
−6.783
5.033
1.00
17.53
N


ATOM
4612
CA
VAL
A
290
46.686
−7.573
5.994
1.00
16.67
C


ATOM
4614
CB
VAL
A
290
46.829
−9.092
5.727
1.00
17.16
C


ATOM
4616
CG1
VAL
A
290
46.181
−9.910
6.839
1.00
16.81
C


ATOM
4620
CG2
VAL
A
290
46.222
−9.460
4.381
1.00
14.02
C


ATOM
4624
C
VAL
A
290
47.115
−7.262
7.427
1.00
17.08
C


ATOM
4625
O
VAL
A
290
46.271
−7.143
8.308
1.00
17.75
O


ATOM
4627
N
THR
A
291
48.424
−7.126
7.645
1.00
17.15
N


ATOM
4628
CA
THR
A
291
48.985
−6.752
8.951
1.00
17.58
C


ATOM
4630
CB
THR
A
291
50.526
−6.575
8.879
1.00
17.62
C


ATOM
4632
OG1
THR
A
291
51.127
−7.762
8.364
1.00
20.13
O


ATOM
4634
CG2
THR
A
291
51.111
−6.283
10.245
1.00
16.15
C


ATOM
4638
C
THR
A
291
48.424
−5.434
9.474
1.00
16.88
C


ATOM
4639
O
THR
A
291
48.019
−5.333
10.628
1.00
17.71
O


ATOM
4641
N
ILE
A
292
48.439
−4.416
8.627
1.00
16.65
N


ATOM
4642
CA
ILE
A
292
47.965
−3.097
9.014
1.00
16.25
C


ATOM
4644
CB
ILE
A
292
48.375
−2.018
7.991
1.00
16.11
C


ATOM
4646
CG1
ILE
A
292
49.900
−1.892
7.956
1.00
16.85
C


ATOM
4649
CD1
ILE
A
292
50.435
−0.771
7.054
1.00
16.26
C


ATOM
4653
CG2
ILE
A
292
47.752
−0.671
8.344
1.00
15.32
C


ATOM
4657
C
ILE
A
292
46.455
−3.112
9.176
1.00
16.13
C


ATOM
4658
O
ILE
A
292
45.943
−2.597
10.161
1.00
16.23
O


ATOM
4660
N
ILE
A
293
45.744
−3.710
8.222
1.00
16.03
N


ATOM
4661
CA
ILE
A
293
44.288
−3.725
8.268
1.00
16.25
C


ATOM
4663
CB
ILE
A
293
43.656
−4.367
7.010
1.00
16.69
C


ATOM
4665
CG1
ILE
A
293
44.080
−3.637
5.725
1.00
15.82
C


ATOM
4668
CD1
ILE
A
293
43.514
−2.279
5.579
1.00
19.58
C


ATOM
4672
CG2
ILE
A
293
42.128
−4.372
7.109
1.00
13.37
C


ATOM
4676
C
ILE
A
293
43.835
−4.478
9.512
1.00
17.75
C


ATOM
4677
O
ILE
A
293
42.946
−4.026
10.226
1.00
17.47
O


ATOM
4679
N
ASP
A
294
44.470
−5.618
9.778
1.00
20.03
N


ATOM
4680
CA
ASP
A
294
44.151
−6.440
10.943
1.00
20.51
C


ATOM
4682
CB
ASP
A
294
45.027
−7.699
10.954
1.00
22.36
C


ATOM
4685
CG
ASP
A
294
44.728
−8.636
12.119
1.00
25.69
C


ATOM
4686
OD1
ASP
A
294
43.622
−8.590
12.702
1.00
37.35
O


ATOM
4687
OD2
ASP
A
294
45.621
−9.442
12.441
1.00
32.79
O


ATOM
4688
C
ASP
A
294
44.336
−5.627
12.213
1.00
19.61
C


ATOM
4689
O
ASP
A
294
43.463
−5.617
13.068
1.00
20.88
O


ATOM
4691
N
ASP
A
295
45.457
−4.926
12.324
1.00
18.57
N


ATOM
4692
CA
ASP
A
295
45.650
−3.975
13.417
1.00
18.59
C


ATOM
4694
CB
ASP
A
295
46.898
−3.111
13.183
1.00
19.56
C


ATOM
4697
CG
ASP
A
295
48.204
−3.851
13.473
1.00
24.22
C


ATOM
4698
OD1
ASP
A
295
48.174
−4.916
14.131
1.00
25.29
O


ATOM
4699
OD2
ASP
A
295
49.272
−3.347
13.048
1.00
28.75
O


ATOM
4700
C
ASP
A
295
44.433
−3.062
13.585
1.00
17.26
C


ATOM
4701
O
ASP
A
295
43.967
−2.842
14.700
1.00
17.26
O


ATOM
4703
N
ILE
A
296
43.921
−2.542
12.473
1.00
16.54
N


ATOM
4704
CA
ILE
A
296
42.797
−1.600
12.495
1.00
15.83
C


ATOM
4706
CB
ILE
A
296
42.503
−1.033
11.086
1.00
15.84
C


ATOM
4708
CG1
ILE
A
296
43.626
−0.086
10.655
1.00
16.73
C


ATOM
4711
CD1
ILE
A
296
43.682
0.168
9.163
1.00
14.31
C


ATOM
4715
CG2
ILE
A
296
41.162
−0.310
11.060
1.00
15.97
C


ATOM
4719
C
ILE
A
296
41.531
−2.234
13.065
1.00
15.34
C


ATOM
4720
O
ILE
A
296
40.844
−1.621
13.878
1.00
14.22
O


ATOM
4722
N
TYR
A
297
41.234
−3.462
12.647
1.00
15.33
N


ATOM
4723
CA
TYR
A
297
40.043
−4.171
13.132
1.00
15.57
C


ATOM
4725
CB
TYR
A
297
39.638
−5.273
12.155
1.00
15.40
C


ATOM
4728
CG
TYR
A
297
38.925
−4.807
10.902
1.00
15.08
C


ATOM
4729
CD1
TYR
A
297
39.630
−4.258
9.840
1.00
15.91
C


ATOM
4731
CE1
TYR
A
297
38.982
−3.851
8.676
1.00
18.67
C


ATOM
4733
CZ
TYR
A
297
37.614
−4.010
8.561
1.00
19.58
C


ATOM
4734
OH
TYR
A
297
36.969
−3.612
7.408
1.00
19.14
O


ATOM
4736
CE2
TYR
A
297
36.894
−4.564
9.605
1.00
19.40
C


ATOM
4738
CD2
TYR
A
297
37.549
−4.963
10.761
1.00
16.57
C


ATOM
4740
C
TYR
A
297
40.234
−4.777
14.529
1.00
15.89
C


ATOM
4741
O
TYR
A
297
39.266
−4.939
15.282
1.00
16.95
O


ATOM
4743
N
ASP
A
298
41.474
−5.111
14.877
1.00
16.19
N


ATOM
4744
CA
ASP
A
298
41.753
−5.797
16.133
1.00
16.54
C


ATOM
4746
CB
ASP
A
298
43.083
−6.545
16.049
1.00
17.40
C


ATOM
4749
CG
ASP
A
298
43.280
−7.525
17.190
1.00
18.90
C


ATOM
4750
OD1
ASP
A
298
42.282
−8.124
17.655
1.00
24.58
O


ATOM
4751
OD2
ASP
A
298
44.444
−7.711
17.605
1.00
23.92
O


ATOM
4752
C
ASP
A
298
41.775
−4.851
17.323
1.00
15.49
C


ATOM
4753
O
ASP
A
298
41.167
−5.135
18.338
1.00
14.90
O


ATOM
4755
N
VAL
A
299
42.472
−3.728
17.197
1.00
15.94
N


ATOM
4756
CA
VAL
A
299
42.685
−2.835
18.346
1.00
15.93
C


ATOM
4758
CB
VAL
A
299
44.133
−2.950
18.885
1.00
16.02
C


ATOM
4760
CG1
VAL
A
299
44.322
−4.270
19.588
1.00
19.85
C


ATOM
4764
CG2
VAL
A
299
45.150
−2.779
17.768
1.00
15.17
C


ATOM
4768
C
VAL
A
299
42.381
−1.352
18.141
1.00
14.96
C


ATOM
4769
O
VAL
A
299
41.961
−0.695
19.085
1.00
16.18
O


ATOM
4771
N
TYR
A
300
42.589
−0.812
16.944
1.00
14.23
N


ATOM
4772
CA
TYR
A
300
42.570
0.645
16.776
1.00
14.38
C


ATOM
4774
CB
TYR
A
300
43.553
1.094
15.685
1.00
14.27
C


ATOM
4777
CG
TYR
A
300
43.766
2.600
15.656
1.00
15.93
C


ATOM
4778
CD1
TYR
A
300
44.629
3.215
16.550
1.00
16.44
C


ATOM
4780
CE1
TYR
A
300
44.825
4.589
16.532
1.00
17.44
C


ATOM
4782
CZ
TYR
A
300
44.148
5.372
15.616
1.00
16.16
C


ATOM
4783
OH
TYR
A
300
44.347
6.732
15.607
1.00
16.93
O


ATOM
4785
CE2
TYR
A
300
43.283
4.793
14.719
1.00
15.86
C


ATOM
4787
CD2
TYR
A
300
43.090
3.410
14.742
1.00
17.88
C


ATOM
4789
C
TYR
A
300
41.182
1.219
16.488
1.00
13.74
C


ATOM
4790
O
TYR
A
300
40.763
2.178
17.130
1.00
13.75
O


ATOM
4792
N
GLY
A
301
40.482
0.641
15.521
1.00
13.36
N


ATOM
4793
CA
GLY
A
301
39.238
1.213
15.030
1.00
13.35
C


ATOM
4796
C
GLY
A
301
38.017
0.879
15.856
1.00
13.38
C


ATOM
4797
O
GLY
A
301
37.901
−0.228
16.373
1.00
15.55
O


ATOM
4799
N
THR
A
302
37.101
1.838
15.975
1.00
13.15
N


ATOM
4800
CA
THR
A
302
35.788
1.584
16.567
1.00
12.98
C


ATOM
4802
CB
THR
A
302
35.041
2.883
16.861
1.00
12.52
C


ATOM
4804
OG1
THR
A
302
34.895
3.621
15.646
1.00
11.42
O


ATOM
4806
CG2
THR
A
302
35.796
3.726
17.877
1.00
9.39
C


ATOM
4810
C
THR
A
302
34.928
0.787
15.597
1.00
13.88
C


ATOM
4811
O
THR
A
302
35.199
0.761
14.392
1.00
13.49
O


ATOM
4813
N
LEU
A
303
33.872
0.166
16.116
1.00
14.79
N


ATOM
4814
CA
LEU
A
303
32.992
−0.669
15.289
1.00
14.49
C


ATOM
4816
CB
LEU
A
303
31.948
−1.382
16.152
1.00
14.62
C


ATOM
4819
CG
LEU
A
303
31.078
−2.460
15.498
1.00
16.79
C


ATOM
4821
CD1
LEU
A
303
31.932
−3.624
14.973
1.00
12.94
C


ATOM
4825
CD2
LEU
A
303
30.026
−2.948
16.493
1.00
11.28
C


ATOM
4829
C
LEU
A
303
32.323
0.157
14.185
1.00
14.27
C


ATOM
4830
O
LEU
A
303
32.179
−0.326
13.069
1.00
12.64
O


ATOM
4832
N
ASP
A
304
31.943
1.400
14.500
1.00
15.41
N


ATOM
4833
CA
ASP
A
304
31.381
2.329
13.510
1.00
15.64
C


ATOM
4835
CB
ASP
A
304
31.055
3.686
14.142
1.00
16.20
C


ATOM
4838
CG
ASP
A
304
29.726
3.698
14.876
1.00
17.84
C


ATOM
4839
OD1
ASP
A
304
29.071
2.641
14.957
1.00
19.62
O


ATOM
4840
OD2
ASP
A
304
29.334
4.782
15.370
1.00
18.67
O


ATOM
4841
C
ASP
A
304
32.332
2.554
12.344
1.00
16.15
C


ATOM
4842
O
ASP
A
304
31.928
2.457
11.183
1.00
16.74
O


ATOM
4844
N
GLU
A
305
33.588
2.864
12.657
1.00
16.40
N


ATOM
4845
CA
GLU
A
305
34.629
3.042
11.635
1.00
16.14
C


ATOM
4847
CB
GLU
A
305
35.956
3.430
12.284
1.00
16.55
C


ATOM
4850
CG
GLU
A
305
35.982
4.814
12.910
1.00
16.50
C


ATOM
4853
CD
GLU
A
305
37.280
5.090
13.653
1.00
15.99
C


ATOM
4854
OE1
GLU
A
305
37.950
4.125
14.080
1.00
11.26
O


ATOM
4855
OE2
GLU
A
305
37.630
6.273
13.813
1.00
14.81
O


ATOM
4856
C
GLU
A
305
34.850
1.773
10.818
1.00
15.75
C


ATOM
4857
O
GLU
A
305
35.061
1.831
9.611
1.00
14.67
O


ATOM
4859
N
LEU
A
306
34.812
0.628
11.488
1.00
16.45
N


ATOM
4860
CA
LEU
A
306
35.040
−0.647
10.819
1.00
17.34
C


ATOM
4862
CB
LEU
A
306
35.154
−1.783
11.840
1.00
16.81
C


ATOM
4865
CG
LEU
A
306
36.360
−1.714
12.780
1.00
14.74
C


ATOM
4867
CD1
LEU
A
306
36.460
−2.987
13.597
1.00
11.90
C


ATOM
4871
CD2
LEU
A
306
37.648
−1.477
12.008
1.00
13.18
C


ATOM
4875
C
LEU
A
306
33.952
−0.951
9.792
1.00
18.32
C


ATOM
4876
O
LEU
A
306
34.236
−1.539
8.748
1.00
18.25
O


ATOM
4878
N
GLU
A
307
32.716
−0.552
10.089
1.00
20.11
N


ATOM
4879
CA
GLU
A
307
31.614
−0.687
9.133
1.00
21.12
C


ATOM
4881
CB
GLU
A
307
30.274
−0.262
9.751
1.00
22.22
C


ATOM
4884
CG
GLU
A
307
29.616
−1.301
10.662
1.00
27.31
C


ATOM
4887
CD
GLU
A
307
28.797
−2.351
9.912
1.00
35.75
C


ATOM
4888
OE1
GLU
A
307
28.483
−2.149
8.717
1.00
40.29
O


ATOM
4889
OE2
GLU
A
307
28.456
−3.385
10.529
1.00
42.00
O


ATOM
4890
C
GLU
A
307
31.901
0.161
7.901
1.00
19.87
C


ATOM
4891
O
GLU
A
307
31.781
−0.319
6.777
1.00
20.57
O


ATOM
4893
N
LEU
A
308
32.291
1.414
8.123
1.00
18.78
N


ATOM
4894
CA
LEU
A
308
32.592
2.336
7.027
1.00
18.39
C


ATOM
4896
CB
LEU
A
308
32.999
3.715
7.559
1.00
17.90
C


ATOM
4899
CG
LEU
A
308
31.906
4.545
8.235
1.00
18.12
C


ATOM
4901
CD1
LEU
A
308
32.457
5.894
8.670
1.00
18.52
C


ATOM
4905
CD2
LEU
A
308
30.716
4.733
7.310
1.00
17.00
C


ATOM
4909
C
LEU
A
308
33.685
1.789
6.118
1.00
17.92
C


ATOM
4910
O
LEU
A
308
33.565
1.843
4.889
1.00
17.37
O


ATOM
4912
N
PHE
A
309
34.739
1.251
6.726
1.00
17.16
N


ATOM
4913
CA
PHE
A
309
35.852
0.703
5.964
1.00
16.57
C


ATOM
4915
CB
PHE
A
309
37.037
0.399
6.876
1.00
16.76
C


ATOM
4918
CG
PHE
A
309
38.317
0.143
6.135
1.00
14.62
C


ATOM
4919
CD1
PHE
A
309
38.645
−1.132
5.705
1.00
13.65
C


ATOM
4921
CE1
PHE
A
309
39.819
−1.368
5.014
1.00
12.54
C


ATOM
4923
CZ
PHE
A
309
40.672
−0.343
4.756
1.00
10.64
C


ATOM
4925
CE2
PHE
A
309
40.361
0.935
5.178
1.00
12.58
C


ATOM
4927
CD2
PHE
A
309
39.187
1.173
5.863
1.00
11.35
C


ATOM
4929
C
PHE
A
309
35.444
−0.552
5.203
1.00
16.22
C


ATOM
4930
O
PHE
A
309
35.823
−0.721
4.053
1.00
15.99
O


ATOM
4932
N
THR
A
310
34.682
−1.433
5.844
1.00
16.65
N


ATOM
4933
CA
THR
A
310
34.201
−2.641
5.180
1.00
16.88
C


ATOM
4935
CB
THR
A
310
33.377
−3.533
6.130
1.00
17.36
C


ATOM
4937
OG1
THR
A
310
34.142
−3.829
7.307
1.00
15.20
O


ATOM
4939
CG2
THR
A
310
32.970
−4.836
5.437
1.00
16.45
C


ATOM
4943
C
THR
A
310
33.343
−2.256
3.978
1.00
17.73
C


ATOM
4944
O
THR
A
310
33.484
−2.821
2.891
1.00
18.32
O


ATOM
4946
N
ASP
A
311
32.468
−1.275
4.186
1.00
18.11
N


ATOM
4947
CA
ASP
A
311
31.586
−0.781
3.135
1.00
17.62
C


ATOM
4949
CB
ASP
A
311
30.621
0.257
3.705
1.00
18.07
C


ATOM
4952
CG
ASP
A
311
29.672
0.799
2.661
1.00
19.82
C


ATOM
4953
OD1
ASP
A
311
28.815
0.024
2.192
1.00
28.04
O


ATOM
4954
OD2
ASP
A
311
29.783
1.994
2.311
1.00
25.85
O


ATOM
4955
C
ASP
A
311
32.391
−0.157
1.996
1.00
17.13
C


ATOM
4956
O
ASP
A
311
32.088
−0.381
0.818
1.00
16.48
O


ATOM
4958
N
ALA
A
312
33.403
0.634
2.360
1.00
15.61
N


ATOM
4959
CA
ALA
A
312
34.294
1.235
1.389
1.00
14.20
C


ATOM
4961
CB
ALA
A
312
35.403
1.959
2.076
1.00
14.10
C


ATOM
4965
C
ALA
A
312
34.861
0.167
0.470
1.00
14.98
C


ATOM
4966
O
ALA
A
312
34.812
0.324
−0.756
1.00
16.36
O


ATOM
4968
N
VAL
A
313
35.365
−0.922
1.058
1.00
14.33
N


ATOM
4969
CA
VAL
A
313
36.002
−1.996
0.290
1.00
14.21
C


ATOM
4971
CB
VAL
A
313
36.804
−2.977
1.189
1.00
14.76
C


ATOM
4973
CG1
VAL
A
313
37.369
−4.145
0.361
1.00
11.15
C


ATOM
4977
CG2
VAL
A
313
37.932
−2.246
1.892
1.00
11.08
C


ATOM
4981
C
VAL
A
313
34.993
−2.769
−0.554
1.00
14.54
C


ATOM
4982
O
VAL
A
313
35.301
−3.130
−1.683
1.00
14.88
O


ATOM
4984
N
GLU
A
314
33.801
−3.018
−0.015
1.00
15.40
N


ATOM
4985
CA
GLU
A
314
32.730
−3.683
−0.771
1.00
16.05
C


ATOM
4987
CB
GLU
A
314
31.485
−3.897
0.101
1.00
16.15
C


ATOM
4990
CG
GLU
A
314
31.607
−5.034
1.117
1.00
17.87
C


ATOM
4993
CD
GLU
A
314
30.407
−5.144
2.062
1.00
21.90
C


ATOM
4994
OE1
GLU
A
314
29.539
−4.244
2.069
1.00
28.09
O


ATOM
4995
OE2
GLU
A
314
30.331
−6.140
2.810
1.00
23.32
O


ATOM
4996
C
GLU
A
314
32.338
−2.896
−2.022
1.00
16.52
C


ATOM
4997
O
GLU
A
314
32.116
−3.473
−3.081
1.00
16.88
O


ATOM
4999
N
ARG
A
315
32.255
−1.579
−1.899
1.00
17.15
N


ATOM
5000
CA
ARG
A
315
31.809
−0.742
−3.008
1.00
18.03
C


ATOM
5002
CB
ARG
A
315
31.191
0.551
−2.474
1.00
18.37
C


ATOM
5005
CG
ARG
A
315
29.910
0.296
−1.684
1.00
24.14
C


ATOM
5008
CD
ARG
A
315
29.125
1.561
−1.389
1.00
30.56
C


ATOM
5011
NE
ARG
A
315
29.929
2.511
−0.629
1.00
38.03
N


ATOM
5013
CZ
ARG
A
315
30.499
3.614
−1.117
1.00
43.73
C


ATOM
5014
NH1
ARG
A
315
30.359
3.966
−2.399
1.00
44.78
N


ATOM
5017
NH2
ARG
A
315
31.213
4.381
−0.300
1.00
44.82
N


ATOM
5020
C
ARG
A
315
32.923
−0.454
−4.016
1.00
17.73
C


ATOM
5021
O
ARG
A
315
32.650
−0.212
−5.188
1.00
18.37
O


ATOM
5023
N
TRP
A
316
34.171
−0.481
−3.556
1.00
17.47
N


ATOM
5024
CA
TRP
A
316
35.338
−0.281
−4.419
1.00
16.53
C


ATOM
5026
CB
TRP
A
316
35.608
−1.537
−5.249
1.00
15.40
C


ATOM
5029
CG
TRP
A
316
37.036
−1.614
−5.669
1.00
14.64
C


ATOM
5030
CD1
TRP
A
316
37.544
−1.323
−6.896
1.00
14.50
C


ATOM
5032
NE1
TRP
A
316
38.906
−1.487
−6.892
1.00
16.54
N


ATOM
5034
CE2
TRP
A
316
39.302
−1.869
−5.640
1.00
14.11
C


ATOM
5035
CD2
TRP
A
316
38.151
−1.956
−4.843
1.00
11.90
C


ATOM
5036
CE3
TRP
A
316
38.283
−2.338
−3.506
1.00
13.84
C


ATOM
5038
CZ3
TRP
A
316
39.537
−2.612
−3.017
1.00
13.63
C


ATOM
5040
CH2
TRP
A
316
40.665
−2.520
−3.836
1.00
17.90
C


ATOM
5042
CZ2
TRP
A
316
40.568
−2.154
−5.151
1.00
17.45
C


ATOM
5044
C
TRP
A
316
35.215
0.967
−5.308
1.00
17.27
C


ATOM
5045
O
TRP
A
316
35.450
0.935
−6.513
1.00
17.36
O


ATOM
5047
N
ASP
A
317
34.845
2.067
−4.671
1.00
19.42
N


ATOM
5048
CA
ASP
A
317
34.645
3.346
−5.320
1.00
20.29
C


ATOM
5050
CB
ASP
A
317
33.186
3.782
−5.133
1.00
20.62
C


ATOM
5053
CG
ASP
A
317
32.924
5.220
−5.560
1.00
21.12
C


ATOM
5054
OD1
ASP
A
317
33.659
5.761
−6.415
1.00
19.88
O


ATOM
5055
OD2
ASP
A
317
31.952
5.805
−5.039
1.00
27.88
O


ATOM
5056
C
ASP
A
317
35.600
4.326
−4.651
1.00
21.82
C


ATOM
5057
O
ASP
A
317
35.543
4.523
−3.442
1.00
21.54
O


ATOM
5059
N
VAL
A
318
36.497
4.908
−5.440
1.00
24.54
N


ATOM
5060
CA
VAL
A
318
37.442
5.892
−4.936
1.00
25.84
C


ATOM
5062
CB
VAL
A
318
38.522
6.234
−5.985
1.00
25.61
C


ATOM
5064
CG1
VAL
A
318
37.930
7.053
−7.128
1.00
22.86
C


ATOM
5068
CG2
VAL
A
318
39.671
6.978
−5.331
1.00
23.84
C


ATOM
5072
C
VAL
A
318
36.719
7.168
−4.534
1.00
28.43
C


ATOM
5073
O
VAL
A
318
37.088
7.792
−3.544
1.00
27.88
O


ATOM
5075
N
ASN
A
319
35.674
7.530
−5.284
1.00
32.04
N


ATOM
5076
CA
ASN
A
319
34.969
8.816
−5.101
1.00
35.31
C


ATOM
5078
CB
ASN
A
319
34.119
9.178
−6.336
1.00
35.75
C


ATOM
5081
CG
ASN
A
319
34.915
9.136
−7.653
1.00
40.12
C


ATOM
5082
OD1
ASN
A
319
35.868
9.902
−7.848
1.00
43.06
O


ATOM
5083
ND2
ASN
A
319
34.508
8.246
−8.567
1.00
34.41
N


ATOM
5086
C
ASN
A
319
34.073
8.811
−3.868
1.00
37.28
C


ATOM
5087
O
ASN
A
319
33.141
9.607
−3.776
1.00
38.17
O


ATOM
5089
N
ALA
A
320
34.336
7.885
−2.947
1.00
39.03
N


ATOM
5090
CA
ALA
A
320
33.756
7.899
−1.615
1.00
40.48
C


ATOM
5092
CB
ALA
A
320
32.559
6.975
−1.550
1.00
40.67
C


ATOM
5096
C
ALA
A
320
34.837
7.489
−0.608
1.00
41.93
C


ATOM
5097
O
ALA
A
320
34.636
6.636
0.254
1.00
42.74
O


ATOM
5099
N
ILE
A
321
36.006
8.095
−0.771
1.00
42.86
N


ATOM
5100
CA
ILE
A
321
37.055
8.073
0.232
1.00
42.96
C


ATOM
5102
CB
ILE
A
321
38.414
8.457
−0.407
1.00
42.98
C


ATOM
5104
CG1
ILE
A
321
39.575
8.031
0.478
1.00
44.97
C


ATOM
5107
CD1
ILE
A
321
40.896
8.642
0.060
1.00
46.59
C


ATOM
5111
CG2
ILE
A
321
38.496
9.958
−0.702
1.00
43.87
C


ATOM
5115
C
ILE
A
321
36.678
9.089
1.314
1.00
43.79
C


ATOM
5116
O
ILE
A
321
36.938
8.870
2.498
1.00
45.72
O


ATOM
5118
N
ASN
A
322
36.024
10.177
0.887
1.00
42.17
N


ATOM
5119
CA
ASN
A
322
35.695
11.340
1.729
1.00
40.19
C


ATOM
5121
CB
ASN
A
322
34.859
12.346
0.922
1.00
40.65
C


ATOM
5124
CG
ASN
A
322
35.585
12.869
−0.313
1.00
42.39
C


ATOM
5125
OD1
ASN
A
322
36.640
13.497
−0.208
1.00
44.33
O


ATOM
5126
ND2
ASN
A
322
35.013
12.619
−1.490
1.00
41.11
N


ATOM
5129
C
ASN
A
322
34.945
11.044
3.028
1.00
37.45
C


ATOM
5130
O
ASN
A
322
35.014
11.824
3.969
1.00
37.12
O


ATOM
5132
N
ASP
A
323
34.229
9.927
3.069
1.00
35.06
N


ATOM
5133
CA
ASP
A
323
33.381
9.576
4.210
1.00
33.48
C


ATOM
5135
CB
ASP
A
323
32.206
8.694
3.745
1.00
35.16
C


ATOM
5138
CG
ASP
A
323
31.671
9.088
2.358
1.00
39.08
C


ATOM
5139
OD1
ASP
A
323
31.625
10.303
2.052
1.00
44.10
O


ATOM
5140
OD2
ASP
A
323
31.310
8.177
1.575
1.00
38.71
O


ATOM
5141
C
ASP
A
323
34.170
8.852
5.313
1.00
30.30
C


ATOM
5142
O
ASP
A
323
33.743
8.825
6.466
1.00
30.13
O


ATOM
5144
N
LEU
A
324
35.309
8.258
4.953
1.00
27.17
N


ATOM
5145
CA
LEU
A
324
36.182
7.573
5.915
1.00
23.84
C


ATOM
5147
CB
LEU
A
324
37.198
6.696
5.181
1.00
22.54
C


ATOM
5150
CG
LEU
A
324
36.673
5.538
4.328
1.00
20.51
C


ATOM
5152
CD1
LEU
A
324
37.803
4.942
3.516
1.00
17.04
C


ATOM
5156
CD2
LEU
A
324
36.012
4.456
5.176
1.00
17.06
C


ATOM
5160
C
LEU
A
324
36.953
8.560
6.802
1.00
22.30
C


ATOM
5161
O
LEU
A
324
37.312
9.648
6.353
1.00
22.20
O


ATOM
5163
N
PRO
A
325
37.232
8.180
8.063
1.00
20.38
N


ATOM
5164
CA
PRO
A
325
38.151
8.993
8.858
1.00
19.43
C


ATOM
5166
CB
PRO
A
325
38.181
8.280
10.218
1.00
19.10
C


ATOM
5169
CG
PRO
A
325
37.658
6.937
9.974
1.00
20.10
C


ATOM
5172
CD
PRO
A
325
36.704
7.045
8.834
1.00
20.49
C


ATOM
5175
C
PRO
A
325
39.548
9.050
8.232
1.00
18.15
C


ATOM
5176
O
PRO
A
325
39.892
8.201
7.421
1.00
17.37
O


ATOM
5177
N
ASP
A
326
40.336
10.047
8.620
1.00
17.88
N


ATOM
5178
CA
ASP
A
326
41.611
10.331
7.958
1.00
17.65
C


ATOM
5180
CB
ASP
A
326
42.367
11.460
8.673
1.00
18.15
C


ATOM
5183
CG
ASP
A
326
41.739
12.825
8.440
1.00
17.32
C


ATOM
5184
OD1
ASP
A
326
40.763
12.890
7.662
1.00
20.36
O


ATOM
5185
OD2
ASP
A
326
42.215
13.826
9.030
1.00
12.44
O


ATOM
5186
C
ASP
A
326
42.513
9.115
7.794
1.00
17.23
C


ATOM
5187
O
ASP
A
326
42.926
8.805
6.673
1.00
17.06
O


ATOM
5189
N
TYR
A
327
42.816
8.418
8.886
1.00
15.83
N


ATOM
5190
CA
TYR
A
327
43.757
7.302
8.791
1.00
14.44
C


ATOM
5192
CB
TYR
A
327
44.073
6.702
10.166
1.00
14.37
C


ATOM
5195
CG
TYR
A
327
42.996
5.836
10.774
1.00
12.94
C


ATOM
5196
CD1
TYR
A
327
41.914
6.400
11.437
1.00
10.39
C


ATOM
5198
CE1
TYR
A
327
40.931
5.611
12.006
1.00
13.24
C


ATOM
5200
CZ
TYR
A
327
41.028
4.239
11.927
1.00
11.47
C


ATOM
5201
OH
TYR
A
327
40.056
3.451
12.496
1.00
9.36
O


ATOM
5203
CE2
TYR
A
327
42.101
3.654
11.284
1.00
11.89
C


ATOM
5205
CD2
TYR
A
327
43.081
4.452
10.719
1.00
7.67
C


ATOM
5207
C
TYR
A
327
43.270
6.242
7.805
1.00
13.74
C


ATOM
5208
O
TYR
A
327
44.073
5.644
7.088
1.00
13.73
O


ATOM
5210
N
MET
A
328
41.959
6.038
7.742
1.00
13.03
N


ATOM
5211
CA
MET
A
328
41.387
5.066
6.815
1.00
13.75
C


ATOM
5213
CB
MET
A
328
39.964
4.686
7.241
1.00
13.89
C


ATOM
5216
CG
MET
A
328
39.903
3.782
8.475
1.00
14.06
C


ATOM
5219
SD
MET
A
328
38.205
3.354
8.967
1.00
13.00
S


ATOM
5220
CE
MET
A
328
38.465
1.789
9.798
1.00
11.82
C


ATOM
5224
C
MET
A
328
41.412
5.544
5.349
1.00
14.27
C


ATOM
5225
O
MET
A
328
41.503
4.720
4.431
1.00
14.37
O


ATOM
5227
N
LYS
A
329
41.326
6.858
5.126
1.00
15.46
N


ATOM
5228
CA
LYS
A
329
41.433
7.424
3.768
1.00
16.19
C


ATOM
5230
CB
LYS
A
329
41.362
8.948
3.781
1.00
16.83
C


ATOM
5233
CG
LYS
A
329
39.973
9.537
3.873
1.00
20.82
C


ATOM
5236
CD
LYS
A
329
40.039
10.924
4.485
1.00
25.45
C


ATOM
5239
CE
LYS
A
329
38.742
11.676
4.337
1.00
27.53
C


ATOM
5242
NZ
LYS
A
329
38.554
12.532
5.521
1.00
27.07
N


ATOM
5246
C
LYS
A
329
42.754
7.035
3.151
1.00
15.90
C


ATOM
5247
O
LYS
A
329
42.813
6.598
2.000
1.00
16.89
O


ATOM
5249
N
LEU
A
330
43.813
7.205
3.933
1.00
14.92
N


ATOM
5250
CA
LEU
A
330
45.153
6.854
3.509
1.00
14.91
C


ATOM
5252
CB
LEU
A
330
46.166
7.329
4.554
1.00
15.21
C


ATOM
5255
CG
LEU
A
330
47.605
7.498
4.083
1.00
16.18
C


ATOM
5257
CD1
LEU
A
330
47.673
8.516
2.958
1.00
16.06
C


ATOM
5261
CD2
LEU
A
330
48.471
7.935
5.243
1.00
17.93
C


ATOM
5265
C
LEU
A
330
45.301
5.350
3.278
1.00
15.28
C


ATOM
5266
O
LEU
A
330
45.853
4.929
2.263
1.00
17.00
O


ATOM
5268
N
ACYS
A
331
44.798
4.546
4.209
0.50
14.63
N


ATOM
5269
N
BCYS
A
331
44.808
4.552
4.224
0.50
15.33
N


ATOM
5270
CA
ACYS
A
331
44.931
3.097
4.121
0.50
13.93
C


ATOM
5271
CA
BCYS
A
331
44.892
3.095
4.144
0.50
15.34
C


ATOM
5274
CB
ACYS
A
331
44.513
2.447
5.439
0.50
14.00
C


ATOM
5275
CB
BCYS
A
331
44.331
2.466
5.420
0.50
15.61
C


ATOM
5280
SG
ACYS
A
331
44.757
0.659
5.474
0.50
9.77
S


ATOM
5281
SG
BCYS
A
331
45.460
2.456
6.817
0.50
17.77
S


ATOM
5284
C
ACYS
A
331
44.114
2.506
2.970
0.50
14.47
C


ATOM
5285
C
BCYS
A
331
44.125
2.537
2.954
0.50
15.16
C


ATOM
5286
O
ACYS
A
331
44.589
1.619
2.257
0.50
14.22
O


ATOM
5287
O
BCYS
A
331
44.640
1.706
2.202
0.50
14.99
O


ATOM
5290
N
PHE
A
332
42.886
2.990
2.801
1.00
15.02
N


ATOM
5291
CA
PHE
A
332
42.007
2.504
1.739
1.00
15.70
C


ATOM
5293
CB
PHE
A
332
40.591
3.086
1.883
1.00
16.91
C


ATOM
5296
CG
PHE
A
332
39.696
2.782
0.715
1.00
18.22
C


ATOM
5297
CD1
PHE
A
332
39.111
1.537
0.583
1.00
20.06
C


ATOM
5299
CE1
PHE
A
332
38.291
1.242
−0.508
1.00
18.34
C


ATOM
5301
CZ
PHE
A
332
38.060
2.197
−1.474
1.00
16.81
C


ATOM
5303
CE2
PHE
A
332
38.648
3.449
−1.356
1.00
19.38
C


ATOM
5305
CD2
PHE
A
332
39.459
3.737
−0.266
1.00
20.23
C


ATOM
5307
C
PHE
A
332
42.566
2.835
0.365
1.00
14.28
C


ATOM
5308
O
PHE
A
332
42.642
1.972
−0.490
1.00
15.28
O


ATOM
5310
N
LEU
A
333
42.976
4.080
0.170
1.00
14.04
N


ATOM
5311
CA
LEU
A
333
43.539
4.513
−1.110
1.00
14.40
C


ATOM
5313
CB
LEU
A
333
43.795
6.026
−1.109
1.00
13.69
C


ATOM
5316
CG
LEU
A
333
44.175
6.650
−2.458
1.00
12.53
C


ATOM
5318
CD1
LEU
A
333
43.315
6.103
−3.568
1.00
9.21
C


ATOM
5322
CD2
LEU
A
333
44.074
8.182
−2.403
1.00
5.83
C


ATOM
5326
C
LEU
A
333
44.827
3.761
−1.472
1.00
14.58
C


ATOM
5327
O
LEU
A
333
45.021
3.408
−2.634
1.00
15.12
O


ATOM
5329
N
ALA
A
334
45.694
3.517
−0.486
1.00
13.94
N


ATOM
5330
CA
ALA
A
334
46.893
2.693
−0.699
1.00
14.13
C


ATOM
5332
CB
ALA
A
334
47.703
2.584
0.585
1.00
12.74
C


ATOM
5336
C
ALA
A
334
46.500
1.298
−1.204
1.00
14.10
C


ATOM
5337
O
ALA
A
334
47.035
0.795
−2.196
1.00
13.54
O


ATOM
5339
N
LEU
A
335
45.540
0.699
−0.515
1.00
14.19
N


ATOM
5340
CA
LEU
A
335
45.011
−0.604
−0.878
1.00
15.38
C


ATOM
5342
CB
LEU
A
335
43.981
−1.032
0.166
1.00
15.83
C


ATOM
5345
CG
LEU
A
335
43.336
−2.395
0.008
1.00
16.89
C


ATOM
5347
CD1
LEU
A
335
44.377
−3.505
0.133
1.00
16.87
C


ATOM
5351
CD2
LEU
A
335
42.260
−2.525
1.063
1.00
15.39
C


ATOM
5355
C
LEU
A
335
44.355
−0.582
−2.260
1.00
15.12
C


ATOM
5356
O
LEU
A
335
44.571
−1.477
−3.084
1.00
14.74
O


ATOM
5358
N
TYR
A
336
43.555
0.450
−2.499
1.00
13.32
N


ATOM
5359
CA
TYR
A
336
42.829
0.594
−3.758
1.00
12.46
C


ATOM
5361
CB
TYR
A
336
42.017
1.881
−3.697
1.00
12.29
C


ATOM
5364
CG
TYR
A
336
41.034
2.111
−4.810
1.00
13.39
C


ATOM
5365
CD1
TYR
A
336
39.785
1.499
−4.795
1.00
15.98
C


ATOM
5367
CE1
TYR
A
336
38.864
1.728
−5.798
1.00
14.25
C


ATOM
5369
CZ
TYR
A
336
39.177
2.594
−6.827
1.00
16.95
C


ATOM
5370
OH
TYR
A
336
38.262
2.826
−7.830
1.00
17.48
O


ATOM
5372
CE2
TYR
A
336
40.408
3.222
−6.855
1.00
17.10
C


ATOM
5374
CD2
TYR
A
336
41.324
2.983
−5.845
1.00
12.44
C


ATOM
5376
C
TYR
A
336
43.801
0.636
−4.936
1.00
11.66
C


ATOM
5377
O
TYR
A
336
43.667
−0.121
−5.895
1.00
10.26
O


ATOM
5379
N
ASN
A
337
44.789
1.521
−4.836
1.00
10.76
N


ATOM
5380
CA
ASN
A
337
45.795
1.674
−5.865
1.00
9.77
C


ATOM
5382
CB
ASN
A
337
46.737
2.822
−5.516
1.00
9.39
C


ATOM
5385
CG
ASN
A
337
46.102
4.182
−5.716
1.00
9.44
C


ATOM
5386
OD1
ASN
A
337
45.004
4.288
−6.251
1.00
15.48
O


ATOM
5387
ND2
ASN
A
337
46.794
5.231
−5.290
1.00
5.51
N


ATOM
5390
C
ASN
A
337
46.593
0.399
−6.062
1.00
10.42
C


ATOM
5391
O
ASN
A
337
46.827
−0.021
−7.189
1.00
12.27
O


ATOM
5393
N
THR
A
338
46.999
−0.228
−4.966
1.00
10.42
N


ATOM
5394
CA
THR
A
338
47.808
−1.437
−5.047
1.00
9.59
C


ATOM
5396
CB
THR
A
338
48.206
−1.935
−3.644
1.00
9.20
C


ATOM
5398
OG1
THR
A
338
49.030
−0.951
−3.013
1.00
10.32
O


ATOM
5400
CG2
THR
A
338
48.971
−3.239
−3.730
1.00
6.16
C


ATOM
5404
C
THR
A
338
47.080
−2.544
−5.810
1.00
9.77
C


ATOM
5405
O
THR
A
338
47.643
−3.123
−6.736
1.00
9.33
O


ATOM
5407
N
ILE
A
339
45.835
−2.822
−5.424
1.00
10.69
N


ATOM
5408
CA
ILE
A
339
45.037
−3.873
−6.066
1.00
11.11
C


ATOM
5410
CB
ILE
A
339
43.731
−4.183
−5.299
1.00
10.99
C


ATOM
5412
CG1
ILE
A
339
44.009
−4.543
−3.832
1.00
11.57
C


ATOM
5415
CD1
ILE
A
339
45.176
−5.465
−3.611
1.00
11.93
C


ATOM
5419
CG2
ILE
A
339
42.969
−5.318
−5.968
1.00
10.25
C


ATOM
5423
C
ILE
A
339
44.702
−3.500
−7.516
1.00
11.94
C


ATOM
5424
O
ILE
A
339
44.814
−4.330
−8.411
1.00
13.78
O


ATOM
5426
N
ASN
A
340
44.321
−2.257
−7.767
1.00
11.14
N


ATOM
5427
CA
ASN
A
340
44.093
−1.845
−9.146
1.00
12.07
C


ATOM
5429
CB
ASN
A
340
43.630
−0.380
−9.236
1.00
11.61
C


ATOM
5432
CG
ASN
A
340
42.224
−0.167
−8.686
1.00
12.18
C


ATOM
5433
OD1
ASN
A
340
41.467
−1.118
−8.486
1.00
12.96
O


ATOM
5434
ND2
ASN
A
340
41.869
1.092
−8.445
1.00
13.65
N


ATOM
5437
C
ASN
A
340
45.347
−2.049
−9.996
1.00
12.28
C


ATOM
5438
O
ASN
A
340
45.253
−2.444
−11.149
1.00
13.92
O


ATOM
5440
N
GLU
A
341
46.518
−1.788
−9.426
1.00
12.30
N


ATOM
5441
CA
AGLU
A
341
47.749
−1.939
−10.195
0.50
12.50
C


ATOM
5442
CA
BGLU
A
341
47.800
−1.937
−10.125
0.50
12.27
C


ATOM
5445
CB
AGLU
A
341
48.922
−1.199
−9.548
0.50
12.74
C


ATOM
5446
CB
BGLU
A
341
48.923
−1.381
−9.235
0.50
12.30
C


ATOM
5451
CG
AGLU
A
341
49.242
0.087
−10.291
0.50
15.08
C


ATOM
5452
CG
BGLU
A
341
50.323
−1.358
−9.837
0.50
13.55
C


ATOM
5457
CD
AGLU
A
341
49.793
1.171
−9.405
0.50
17.89
C


ATOM
5458
CD
BGLU
A
341
51.343
−0.733
−8.887
0.50
14.23
C


ATOM
5459
OE1
AGLU
A
341
49.712
1.030
−8.165
0.50
20.72
O


ATOM
5460
OE1
BGLU
A
341
51.062
−0.650
−7.667
0.50
15.78
O


ATOM
5461
OE2
AGLU
A
341
50.298
2.170
−9.958
0.50
17.56
O


ATOM
5462
OE2
BGLU
A
341
52.421
−0.319
−9.358
0.50
11.79
O


ATOM
5463
C
GLU
A
341
48.068
−3.404
−10.477
1.00
12.01
C


ATOM
5464
O
GLU
A
341
48.536
−3.729
−11.560
1.00
11.80
O


ATOM
5466
N
ILE
A
342
47.776
−4.295
−9.538
1.00
12.43
N


ATOM
5467
CA
ILE
A
342
47.925
−5.726
−9.800
1.00
12.18
C


ATOM
5469
CB
ILE
A
342
47.743
−6.566
−8.528
1.00
11.60
C


ATOM
5471
CG1
ILE
A
342
48.871
−6.291
−7.527
1.00
12.00
C


ATOM
5474
CD1
ILE
A
342
48.615
−6.861
−6.144
1.00
10.85
C


ATOM
5478
CG2
ILE
A
342
47.726
−8.034
−8.863
1.00
8.35
C


ATOM
5482
C
ILE
A
342
46.919
−6.152
−10.887
1.00
13.54
C


ATOM
5483
O
ILE
A
342
47.251
−6.943
−11.777
1.00
15.29
O


ATOM
5485
N
ALA
A
343
45.702
−5.611
−10.829
1.00
13.12
N


ATOM
5486
CA
ALA
A
343
44.718
−5.832
−11.890
1.00
12.52
C


ATOM
5488
CB
ALA
A
343
43.398
−5.134
−11.569
1.00
11.07
C


ATOM
5492
C
ALA
A
343
45.249
−5.362
−13.246
1.00
12.15
C


ATOM
5493
O
ALA
A
343
45.048
−6.035
−14.258
1.00
13.04
O


ATOM
5495
N
TYR
A
344
45.924
−4.217
−13.265
1.00
11.46
N


ATOM
5496
CA
TYR
A
344
46.483
−3.695
−14.509
1.00
11.43
C


ATOM
5498
CB
TYR
A
344
47.136
−2.333
−14.301
1.00
10.28
C


ATOM
5501
CG
TYR
A
344
47.745
−1.804
−15.563
1.00
6.11
C


ATOM
5502
CD1
TYR
A
344
46.979
−1.106
−16.488
1.00
5.19
C


ATOM
5504
CE1
TYR
A
344
47.540
−0.630
−17.671
1.00
5.77
C


ATOM
5506
CZ
TYR
A
344
48.874
−0.861
−17.933
1.00
3.91
C


ATOM
5507
OH
TYR
A
344
49.432
−0.399
−19.099
1.00
8.41
O


ATOM
5509
CE2
TYR
A
344
49.652
−1.549
−17.027
1.00
3.93
C


ATOM
5511
CD2
TYR
A
344
49.084
−2.022
−15.852
1.00
5.43
C


ATOM
5513
C
TYR
A
344
47.514
−4.656
−15.080
1.00
13.12
C


ATOM
5514
O
TYR
A
344
47.511
−4.944
−16.271
1.00
12.48
O


ATOM
5516
N
ASP
A
345
48.400
−5.148
−14.215
1.00
14.96
N


ATOM
5517
CA
ASP
A
345
49.454
−6.073
−14.632
1.00
14.86
C


ATOM
5519
CB
ASP
A
345
50.278
−6.552
−13.428
1.00
14.44
C


ATOM
5522
CG
ASP
A
345
51.136
−5.446
−12.817
1.00
16.57
C


ATOM
5523
OD1
ASP
A
345
51.487
−4.467
−13.513
1.00
18.74
O


ATOM
5524
OD2
ASP
A
345
51.466
−5.561
−11.625
1.00
23.50
O


ATOM
5525
C
ASP
A
345
48.855
−7.258
−15.365
1.00
14.39
C


ATOM
5526
O
ASP
A
345
49.292
−7.592
−16.458
1.00
14.24
O


ATOM
5528
N
ASN
A
346
47.841
−7.874
−14.763
1.00
15.32
N


ATOM
5529
CA
ASN
A
346
47.186
−9.042
−15.347
1.00
14.92
C


ATOM
5531
CB
ASN
A
346
46.266
−9.713
−14.339
1.00
14.83
C


ATOM
5534
CG
ASN
A
346
47.026
−10.475
−13.303
1.00
15.26
C


ATOM
5535
OD1
ASN
A
346
47.258
−11.672
−13.453
1.00
19.10
O


ATOM
5536
ND2
ASN
A
346
47.469
−9.782
−12.260
1.00
16.18
N


ATOM
5539
C
ASN
A
346
46.409
−8.719
−16.609
1.00
15.60
C


ATOM
5540
O
ASN
A
346
46.378
−9.527
−17.544
1.00
16.80
O


ATOM
5542
N
LEU
A
347
45.787
−7.546
−16.648
1.00
14.76
N


ATOM
5543
CA
LEU
A
347
45.130
−7.112
−17.868
1.00
14.61
C


ATOM
5545
CB
LEU
A
347
44.352
−5.809
−17.651
1.00
14.92
C


ATOM
5548
CG
LEU
A
347
43.721
−5.218
−18.914
1.00
14.84
C


ATOM
5550
CD1
LEU
A
347
42.715
−6.190
−19.497
1.00
11.67
C


ATOM
5554
CD2
LEU
A
347
43.088
−3.876
−18.614
1.00
11.92
C


ATOM
5558
C
LEU
A
347
46.178
−6.947
−18.965
1.00
14.97
C


ATOM
5559
O
LEU
A
347
45.967
−7.398
−20.081
1.00
15.96
O


ATOM
5561
N
LYS
A
348
47.307
−6.318
−18.640
1.00
15.37
N


ATOM
5562
CA
LYS
A
348
48.382
−6.112
−19.613
1.00
16.52
C


ATOM
5564
CB
LYS
A
348
49.518
−5.265
−19.022
1.00
16.29
C


ATOM
5567
CG
LYS
A
348
50.569
−4.841
−20.050
1.00
17.74
C


ATOM
5570
CD
LYS
A
348
51.624
−3.879
−19.481
1.00
21.53
C


ATOM
5573
CE
LYS
A
348
52.924
−4.574
−19.086
1.00
23.19
C


ATOM
5576
NZ
LYS
A
348
52.791
−5.374
−17.844
1.00
26.07
N


ATOM
5580
C
LYS
A
348
48.953
−7.440
−20.109
1.00
17.40
C


ATOM
5581
O
LYS
A
348
49.088
−7.645
−21.311
1.00
16.65
O


ATOM
5583
N
ASP
A
349
49.280
−8.336
−19.185
1.00
18.21
N


ATOM
5584
CA
ASP
A
349
50.060
−9.521
−19.535
1.00
20.43
C


ATOM
5586
CB
ASP
A
349
51.031
−9.873
−18.400
1.00
20.31
C


ATOM
5589
CG
ASP
A
349
52.074
−8.793
−18.172
1.00
21.16
C


ATOM
5590
OD1
ASP
A
349
52.285
−7.949
−19.075
1.00
24.16
O


ATOM
5591
OD2
ASP
A
349
52.681
−8.780
−17.085
1.00
25.79
O


ATOM
5592
C
ASP
A
349
49.221
−10.740
−19.927
1.00
21.21
C


ATOM
5593
O
ASP
A
349
49.687
−11.572
−20.707
1.00
21.34
O


ATOM
5595
N
LYS
A
350
47.997
−10.841
−19.403
1.00
21.88
N


ATOM
5596
CA
LYS
A
350
47.118
−11.991
−19.694
1.00
21.16
C


ATOM
5598
CB
LYS
A
350
46.741
−12.705
−18.403
1.00
20.09
C


ATOM
5601
CG
LYS
A
350
47.926
−13.179
−17.600
1.00
24.60
C


ATOM
5604
CD
LYS
A
350
47.459
−13.855
−16.313
1.00
31.14
C


ATOM
5607
CE
LYS
A
350
48.615
−14.176
−15.384
1.00
32.30
C


ATOM
5610
NZ
LYS
A
350
48.163
−14.116
−13.979
1.00
29.99
N


ATOM
5614
C
LYS
A
350
45.836
−11.644
−20.454
1.00
20.16
C


ATOM
5615
O
LYS
A
350
45.106
−12.539
−20.854
1.00
20.90
O


ATOM
5617
N
GLY
A
351
45.549
−10.363
−20.639
1.00
19.21
N


ATOM
5618
CA
GLY
A
351
44.363
−9.955
−21.388
1.00
18.87
C


ATOM
5621
C
GLY
A
351
43.044
−10.298
−20.716
1.00
18.23
C


ATOM
5622
O
GLY
A
351
42.040
−10.496
−21.391
1.00
17.31
O


ATOM
5624
N
GLU
A
352
43.044
−10.371
−19.388
1.00
18.71
N


ATOM
5625
CA
GLU
A
352
41.823
−10.635
−18.628
1.00
19.80
C


ATOM
5627
CB
GLU
A
352
41.901
−11.988
−17.912
1.00
20.50
C


ATOM
5630
CG
GLU
A
352
42.232
−13.192
−18.813
1.00
23.98
C


ATOM
5633
CD
GLU
A
352
41.035
−13.738
−19.579
1.00
29.52
C


ATOM
5634
OE1
GLU
A
352
40.005
−13.031
−19.692
1.00
32.18
O


ATOM
5635
OE2
GLU
A
352
41.134
−14.881
−20.080
1.00
35.96
O


ATOM
5636
C
GLU
A
352
41.603
−9.547
−17.592
1.00
19.63
C


ATOM
5637
O
GLU
A
352
42.551
−8.960
−17.082
1.00
20.46
O


ATOM
5639
N
ASN
A
353
40.341
−9.278
−17.290
1.00
19.72
N


ATOM
5640
CA
ASN
A
353
39.981
−8.404
−16.187
1.00
18.98
C


ATOM
5642
CB
ASN
A
353
38.768
−7.557
−16.551
1.00
18.32
C


ATOM
5645
CG
ASN
A
353
38.301
−6.702
−15.406
1.00
20.99
C


ATOM
5646
OD1
ASN
A
353
38.931
−6.667
−14.337
1.00
20.74
O


ATOM
5647
ND2
ASN
A
353
37.184
−6.006
−15.609
1.00
22.54
N


ATOM
5650
C
ASN
A
353
39.686
−9.246
−14.953
1.00
17.99
C


ATOM
5651
O
ASN
A
353
38.593
−9.773
−14.805
1.00
18.92
O


ATOM
5653
N
ILE
A
354
40.668
−9.357
−14.067
1.00
16.81
N


ATOM
5654
CA
ILE
A
354
40.537
−10.173
−12.864
1.00
15.17
C


ATOM
5656
CB
ILE
A
354
41.836
−10.976
−12.586
1.00
15.39
C


ATOM
5658
CG1
ILE
A
354
43.010
−10.051
−12.225
1.00
17.00
C


ATOM
5661
CD1
ILE
A
354
44.125
−10.749
−11.461
1.00
20.37
C


ATOM
5665
CG2
ILE
A
354
42.196
−11.815
−13.782
1.00
12.65
C


ATOM
5669
C
ILE
A
354
40.180
−9.331
−11.629
1.00
14.45
C


ATOM
5670
O
ILE
A
354
40.099
−9.847
−10.511
1.00
12.79
O


ATOM
5672
N
LEU
A
355
39.950
−8.039
−11.835
1.00
14.19
N


ATOM
5673
CA
LEU
A
355
39.719
−7.129
−10.718
1.00
14.17
C


ATOM
5675
CB
LEU
A
355
39.480
−5.705
−11.231
1.00
12.42
C


ATOM
5678
CG
LEU
A
355
39.391
−4.650
−10.137
1.00
11.47
C


ATOM
5680
CD1
LEU
A
355
40.646
−4.678
−9.292
1.00
6.95
C


ATOM
5684
CD2
LEU
A
355
39.148
−3.272
−10.730
1.00
9.67
C


ATOM
5688
C
LEU
A
355
38.587
−7.565
−9.758
1.00
14.65
C


ATOM
5689
O
LEU
A
355
38.759
−7.508
−8.543
1.00
14.60
O


ATOM
5691
N
PRO
A
356
37.434
−8.006
−10.293
1.00
15.55
N


ATOM
5692
CA
PRO
A
356
36.341
−8.425
−9.401
1.00
15.63
C


ATOM
5694
CB
PRO
A
356
35.284
−8.968
−10.370
1.00
15.81
C


ATOM
5697
CG
PRO
A
356
35.536
−8.248
−11.636
1.00
15.52
C


ATOM
5700
CD
PRO
A
356
37.035
−8.089
−11.710
1.00
15.21
C


ATOM
5703
C
PRO
A
356
36.731
−9.505
−8.411
1.00
15.51
C


ATOM
5704
O
PRO
A
356
36.235
−9.512
−7.295
1.00
16.14
O


ATOM
5705
N
TYR
A
357
37.617
−10.404
−8.825
1.00
16.37
N


ATOM
5706
CA
TYR
A
357
38.049
−11.519
−7.982
1.00
16.67
C


ATOM
5708
CB
TYR
A
357
38.720
−12.603
−8.830
1.00
17.29
C


ATOM
5711
CG
TYR
A
357
37.909
−13.005
−10.052
1.00
21.26
C


ATOM
5712
CD1
TYR
A
357
36.674
−13.619
−9.917
1.00
25.64
C


ATOM
5714
CE1
TYR
A
357
35.927
−13.982
−11.025
1.00
25.97
C


ATOM
5716
CZ
TYR
A
357
36.409
−13.730
−12.287
1.00
27.46
C


ATOM
5717
OH
TYR
A
357
35.657
−14.100
−13.376
1.00
34.19
O


ATOM
5719
CE2
TYR
A
357
37.632
−13.118
−12.455
1.00
25.17
C


ATOM
5721
CD2
TYR
A
357
38.376
−12.761
−11.339
1.00
25.11
C


ATOM
5723
C
TYR
A
357
38.984
−11.063
−6.861
1.00
16.92
C


ATOM
5724
O
TYR
A
357
38.933
−11.603
−5.754
1.00
18.10
O


ATOM
5726
N
LEU
A
358
39.823
−10.066
−7.148
1.00
17.16
N


ATOM
5727
CA
LEU
A
358
40.752
−9.498
−6.158
1.00
16.20
C


ATOM
5729
CB
LEU
A
358
41.808
−8.629
−6.844
1.00
16.00
C


ATOM
5732
CG
LEU
A
358
42.646
−9.282
−7.950
1.00
14.84
C


ATOM
5734
CD1
LEU
A
358
43.805
−8.382
−8.330
1.00
10.56
C


ATOM
5738
CD2
LEU
A
358
43.170
−10.648
−7.512
1.00
12.82
C


ATOM
5742
C
LEU
A
358
40.010
−8.664
−5.124
1.00
16.18
C


ATOM
5743
O
LEU
A
358
40.300
−8.731
−3.928
1.00
15.89
O


ATOM
5745
N
THR
A
359
39.043
−7.885
−5.592
1.00
16.77
N


ATOM
5746
CA
THR
A
359
38.249
−7.034
−4.720
1.00
17.41
C


ATOM
5748
CB
THR
A
359
37.443
−6.006
−5.536
1.00
16.65
C


ATOM
5750
OG1
THR
A
359
36.632
−6.687
−6.489
1.00
18.62
O


ATOM
5752
CG2
THR
A
359
38.374
−5.068
−6.291
1.00
17.08
C


ATOM
5756
C
THR
A
359
37.325
−7.889
−3.845
1.00
18.90
C


ATOM
5757
O
THR
A
359
37.211
−7.661
−2.642
1.00
19.65
O


ATOM
5759
N
LYS
A
360
36.685
−8.889
−4.441
1.00
19.60
N


ATOM
5760
CA
LYS
A
360
35.891
−9.848
−3.668
1.00
20.45
C


ATOM
5762
CB
LYS
A
360
35.377
−10.962
−4.580
1.00
20.46
C


ATOM
5765
CG
LYS
A
360
34.546
−12.035
−3.897
1.00
24.40
C


ATOM
5768
CD
LYS
A
360
33.185
−11.503
−3.435
1.00
30.15
C


ATOM
5771
CE
LYS
A
360
32.364
−12.598
−2.738
1.00
31.64
C


ATOM
5774
NZ
LYS
A
360
31.450
−12.051
−1.693
1.00
28.80
N


ATOM
5778
C
LYS
A
360
36.713
−10.438
−2.511
1.00
20.23
C


ATOM
5779
O
LYS
A
360
36.252
−10.486
−1.367
1.00
20.87
O


ATOM
5781
N
ALA
A
361
37.933
−10.876
−2.817
1.00
19.45
N


ATOM
5782
CA
ALA
A
361
38.849
−11.396
−1.801
1.00
18.14
C


ATOM
5784
CB
ALA
A
361
40.219
−11.632
−2.401
1.00
17.05
C


ATOM
5788
C
ALA
A
361
38.959
−10.452
−0.597
1.00
18.20
C


ATOM
5789
O
ALA
A
361
38.850
−10.889
0.554
1.00
18.81
O


ATOM
5791
N
TRP
A
362
39.168
−9.164
−0.869
1.00
16.65
N


ATOM
5792
CA
TRP
A
362
39.345
−8.168
0.194
1.00
15.23
C


ATOM
5794
CB
TRP
A
362
39.963
−6.887
−0.363
1.00
14.11
C


ATOM
5797
CG
TRP
A
362
41.421
−6.998
−0.437
1.00
12.95
C


ATOM
5798
CD1
TRP
A
362
42.179
−7.247
−1.540
1.00
9.85
C


ATOM
5800
NE1
TRP
A
362
43.499
−7.320
−1.196
1.00
12.46
N


ATOM
5802
CE2
TRP
A
362
43.612
−7.121
0.156
1.00
14.43
C


ATOM
5803
CD2
TRP
A
362
42.320
−6.914
0.660
1.00
14.03
C


ATOM
5804
CE3
TRP
A
362
42.155
−6.685
2.029
1.00
14.93
C


ATOM
5806
CZ3
TRP
A
362
43.267
−6.663
2.830
1.00
14.69
C


ATOM
5808
CH2
TRP
A
362
44.542
−6.866
2.299
1.00
14.56
C


ATOM
5810
CZ2
TRP
A
362
44.735
−7.094
0.968
1.00
15.89
C


ATOM
5812
C
TRP
A
362
38.054
−7.854
0.949
1.00
15.33
C


ATOM
5813
O
TRP
A
362
38.079
−7.629
2.160
1.00
14.82
O


ATOM
5815
N
ALA
A
363
36.936
−7.830
0.231
1.00
15.17
N


ATOM
5816
CA
ALA
A
363
35.630
−7.651
0.849
1.00
14.51
C


ATOM
5818
CB
ALA
A
363
34.540
−7.591
−0.216
1.00
12.83
C


ATOM
5822
C
ALA
A
363
35.372
−8.798
1.831
1.00
14.93
C


ATOM
5823
O
ALA
A
363
34.947
−8.562
2.966
1.00
14.26
O


ATOM
5825
N
ASP
A
364
35.657
−10.029
1.394
1.00
15.09
N


ATOM
5826
CA
ASP
A
364
35.445
−11.221
2.219
1.00
14.96
C


ATOM
5828
CB
ASP
A
364
35.697
−12.506
1.420
1.00
14.65
C


ATOM
5831
CG
ASP
A
364
34.607
−12.792
0.386
1.00
15.43
C


ATOM
5832
OD1
ASP
A
364
33.604
−12.050
0.292
1.00
18.73
O


ATOM
5833
OD2
ASP
A
364
34.761
−13.775
−0.356
1.00
22.17
O


ATOM
5834
C
ASP
A
364
36.323
−11.204
3.460
1.00
15.67
C


ATOM
5835
O
ASP
A
364
35.844
−11.466
4.565
1.00
17.80
O


ATOM
5837
N
LEU
A
365
37.598
−10.873
3.289
1.00
15.88
N


ATOM
5838
CA
LEU
A
365
38.510
−10.769
4.423
1.00
16.76
C


ATOM
5840
CB
LEU
A
365
39.925
−10.452
3.949
1.00
17.27
C


ATOM
5843
CG
LEU
A
365
40.998
−10.353
5.036
1.00
17.42
C


ATOM
5845
CD1
LEU
A
365
40.987
−11.590
5.902
1.00
12.95
C


ATOM
5849
CD2
LEU
A
365
42.368
−10.142
4.411
1.00
17.41
C


ATOM
5853
C
LEU
A
365
38.064
−9.687
5.391
1.00
18.04
C


ATOM
5854
O
LEU
A
365
38.058
−9.887
6.613
1.00
19.09
O


ATOM
5856
N
CYS
A
366
37.692
−8.537
4.841
1.00
18.42
N


ATOM
5857
CA
CYS
A
366
37.265
−7.417
5.664
1.00
18.05
C


ATOM
5859
CB
CYS
A
366
37.106
−6.141
4.829
1.00
18.27
C


ATOM
5862
SG
CYS
A
366
38.695
−5.330
4.422
1.00
17.97
S


ATOM
5864
C
CYS
A
366
35.987
−7.755
6.414
1.00
17.44
C


ATOM
5865
O
CYS
A
366
35.820
−7.355
7.559
1.00
18.14
O


ATOM
5867
N
ASN
A
367
35.092
−8.508
5.790
1.00
17.59
N


ATOM
5868
CA
ASN
A
367
33.872
−8.920
6.486
1.00
17.07
C


ATOM
5870
CB
ASN
A
367
32.826
−9.441
5.510
1.00
15.44
C


ATOM
5873
CG
ASN
A
367
31.893
−8.359
5.058
1.00
16.48
C


ATOM
5874
OD1
ASN
A
367
31.084
−7.857
5.844
1.00
21.26
O


ATOM
5875
ND2
ASN
A
367
32.004
−7.972
3.798
1.00
14.60
N


ATOM
5878
C
ASN
A
367
34.149
−9.942
7.579
1.00
17.69
C


ATOM
5879
O
ASN
A
367
33.453
−9.961
8.596
1.00
18.52
O


ATOM
5881
N
ALA
A
368
35.162
−10.781
7.370
1.00
17.11
N


ATOM
5882
CA
ALA
A
368
35.560
−11.751
8.381
1.00
16.83
C


ATOM
5884
CB
ALA
A
368
36.539
−12.765
7.802
1.00
16.79
C


ATOM
5888
C
ALA
A
368
36.172
−11.040
9.584
1.00
16.35
C


ATOM
5889
O
ALA
A
368
35.901
−11.406
10.725
1.00
15.97
O


ATOM
5891
N
PHE
A
369
37.002
−10.030
9.333
1.00
16.26
N


ATOM
5892
CA
PHE
A
369
37.503
−9.200
10.427
1.00
16.89
C


ATOM
5894
CB
PHE
A
369
38.446
−8.088
9.943
1.00
16.81
C


ATOM
5897
CG
PHE
A
369
39.788
−8.565
9.461
1.00
19.32
C


ATOM
5898
CD1
PHE
A
369
40.414
−9.671
10.022
1.00
21.86
C


ATOM
5900
CE1
PHE
A
369
41.655
−10.081
9.572
1.00
21.61
C


ATOM
5902
CZ
PHE
A
369
42.292
−9.380
8.577
1.00
23.30
C


ATOM
5904
CE2
PHE
A
369
41.694
−8.260
8.031
1.00
21.86
C


ATOM
5906
CD2
PHE
A
369
40.456
−7.858
8.474
1.00
20.80
C


ATOM
5908
C
PHE
A
369
36.339
−8.543
11.152
1.00
15.84
C


ATOM
5909
O
PHE
A
369
36.302
−8.514
12.375
1.00
16.60
O


ATOM
5911
N
LEU
A
370
35.400
−8.004
10.386
1.00
15.25
N


ATOM
5912
CA
LEU
A
370
34.288
−7.259
10.954
1.00
15.74
C


ATOM
5914
CB
LEU
A
370
33.418
−6.679
9.842
1.00
15.97
C


ATOM
5917
CG
LEU
A
370
32.208
−5.850
10.264
1.00
16.08
C


ATOM
5919
CD1
LEU
A
370
32.618
−4.682
11.162
1.00
13.02
C


ATOM
5923
CD2
LEU
A
370
31.495
−5.358
9.018
1.00
15.46
C


ATOM
5927
C
LEU
A
370
33.456
−8.151
11.857
1.00
14.86
C


ATOM
5928
O
LEU
A
370
33.019
−7.724
12.922
1.00
13.61
O


ATOM
5930
N
GLN
A
371
33.248
−9.388
11.415
1.00
14.96
N


ATOM
5931
CA
GLN
A
371
32.540
−10.379
12.204
1.00
15.51
C


ATOM
5933
CB
GLN
A
371
32.484
−11.722
11.464
1.00
15.87
C


ATOM
5936
CG
GLN
A
371
31.720
−12.828
12.202
1.00
16.69
C


ATOM
5939
CD
GLN
A
371
30.310
−12.407
12.564
1.00
16.34
C


ATOM
5940
OE1
GLN
A
371
30.058
−11.910
13.665
1.00
18.89
O


ATOM
5941
NE2
GLN
A
371
29.388
−12.570
11.626
1.00
18.01
N


ATOM
5944
C
GLN
A
371
33.219
−10.558
13.555
1.00
15.77
C


ATOM
5945
O
GLN
A
371
32.542
−10.625
14.586
1.00
16.34
O


ATOM
5947
N
GLU
A
372
34.550
−10.634
13.543
1.00
15.39
N


ATOM
5948
CA
GLU
A
372
35.314
−10.832
14.772
1.00
15.88
C


ATOM
5950
CB
GLU
A
372
36.797
−11.110
14.478
1.00
16.07
C


ATOM
5953
CG
GLU
A
372
37.080
−12.546
14.058
1.00
19.38
C


ATOM
5956
CD
GLU
A
372
38.513
−12.769
13.600
1.00
24.01
C


ATOM
5957
OE1
GLU
A
372
39.316
−11.816
13.649
1.00
32.49
O


ATOM
5958
OE2
GLU
A
372
38.841
−13.905
13.193
1.00
29.37
O


ATOM
5959
C
GLU
A
372
35.154
−9.641
15.711
1.00
15.23
C


ATOM
5960
O
GLU
A
372
34.964
−9.820
16.909
1.00
15.07
O


ATOM
5962
N
ALA
A
373
35.216
−8.435
15.157
1.00
15.04
N


ATOM
5963
CA
ALA
A
373
34.969
−7.225
15.927
1.00
15.94
C


ATOM
5965
CB
ALA
A
373
35.169
−5.989
15.066
1.00
14.89
C


ATOM
5969
C
ALA
A
373
33.558
−7.251
16.495
1.00
16.92
C


ATOM
5970
O
ALA
A
373
33.355
−6.992
17.681
1.00
17.49
O


ATOM
5972
N
LYS
A
374
32.586
−7.584
15.652
1.00
17.90
N


ATOM
5973
CA
LYS
A
374
31.191
−7.622
16.083
1.00
19.15
C


ATOM
5975
CB
LYS
A
374
30.255
−7.953
14.920
1.00
20.40
C


ATOM
5978
CG
LYS
A
374
29.877
−6.757
14.066
1.00
23.48
C


ATOM
5981
CD
LYS
A
374
28.920
−7.172
12.962
1.00
28.64
C


ATOM
5984
CE
LYS
A
374
28.478
−5.991
12.113
1.00
29.85
C


ATOM
5987
NZ
LYS
A
374
27.970
−6.432
10.777
1.00
29.46
N


ATOM
5991
C
LYS
A
374
30.971
−8.612
17.216
1.00
18.39
C


ATOM
5992
O
LYS
A
374
30.240
−8.312
18.148
1.00
18.53
O


ATOM
5994
N
TRP
A
375
31.602
−9.782
17.138
1.00
17.94
N


ATOM
5995
CA
TRP
A
375
31.497
−10.780
18.208
1.00
17.60
C


ATOM
5997
CB
TRP
A
375
32.153
−12.107
17.803
1.00
17.34
C


ATOM
6000
CG
TRP
A
375
31.346
−12.981
16.887
1.00
14.45
C


ATOM
6001
CD1
TRP
A
375
29.982
−13.070
16.812
1.00
16.34
C


ATOM
6003
NE1
TRP
A
375
29.617
−14.007
15.875
1.00
13.18
N


ATOM
6005
CE2
TRP
A
375
30.749
−14.557
15.337
1.00
12.63
C


ATOM
6006
CD2
TRP
A
375
31.859
−13.938
15.958
1.00
12.50
C


ATOM
6007
CE3
TRP
A
375
33.147
−14.329
15.582
1.00
11.36
C


ATOM
6009
CZ3
TRP
A
375
33.289
−15.304
14.610
1.00
13.99
C


ATOM
6011
CH2
TRP
A
375
32.166
−15.898
14.005
1.00
15.83
C


ATOM
6013
CZ2
TRP
A
375
30.891
−15.540
14.355
1.00
13.88
C


ATOM
6015
C
TRP
A
375
32.137
−10.279
19.502
1.00
18.44
C


ATOM
6016
O
TRP
A
375
31.573
−10.448
20.583
1.00
18.39
O


ATOM
6018
N
LEU
A
376
33.314
−9.669
19.381
1.00
19.75
N


ATOM
6019
CA
LEU
A
376
34.029
−9.118
20.532
1.00
21.68
C


ATOM
6021
CB
LEU
A
376
35.381
−8.530
20.107
1.00
23.12
C


ATOM
6024
CG
LEU
A
376
36.368
−8.303
21.266
1.00
30.40
C


ATOM
6026
CD1
LEU
A
376
37.399
−9.451
21.302
1.00
34.97
C


ATOM
6030
CD2
LEU
A
376
37.071
−6.929
21.198
1.00
31.18
C


ATOM
6034
C
LEU
A
376
33.196
−8.035
21.219
1.00
20.89
C


ATOM
6035
O
LEU
A
376
33.004
−8.069
22.430
1.00
21.07
O


ATOM
6037
N
TYR
A
377
32.698
−7.092
20.428
1.00
20.33
N


ATOM
6038
CA
TYR
A
377
31.883
−5.993
20.927
1.00
20.67
C


ATOM
6040
CB
TYR
A
377
31.477
−5.076
19.772
1.00
20.85
C


ATOM
6043
CG
TYR
A
377
30.673
−3.867
20.205
1.00
22.25
C


ATOM
6044
CD1
TYR
A
377
31.306
−2.683
20.572
1.00
23.31
C


ATOM
6046
CE1
TYR
A
377
30.579
−1.575
20.969
1.00
23.53
C


ATOM
6048
CZ
TYR
A
377
29.198
−1.638
21.000
1.00
24.30
C


ATOM
6049
OH
TYR
A
377
28.474
−0.538
21.402
1.00
26.73
O


ATOM
6051
CE2
TYR
A
377
28.543
−2.801
20.639
1.00
22.45
C


ATOM
6053
CD2
TYR
A
377
29.280
−3.908
20.249
1.00
22.07
C


ATOM
6055
C
TYR
A
377
30.620
−6.464
21.644
1.00
21.34
C


ATOM
6056
O
TYR
A
377
30.291
−5.967
22.726
1.00
21.24
O


ATOM
6058
N
ASN
A
378
29.907
−7.407
21.029
1.00
21.84
N


ATOM
6059
CA
ASN
A
378
28.639
−7.905
21.575
1.00
22.09
C


ATOM
6061
CB
ASN
A
378
27.737
−8.422
20.445
1.00
21.73
C


ATOM
6064
CG
ASN
A
378
27.166
−7.300
19.594
1.00
20.87
C


ATOM
6065
OD1
ASN
A
378
26.647
−6.315
20.116
1.00
27.66
O


ATOM
6066
ND2
ASN
A
378
27.252
−7.445
18.286
1.00
17.61
N


ATOM
6069
C
ASN
A
378
28.827
−8.985
22.642
1.00
22.65
C


ATOM
6070
O
ASN
A
378
27.853
−9.461
23.215
1.00
21.54
O


ATOM
6072
N
LYS
A
379
30.084
−9.350
22.904
1.00
24.61
N


ATOM
6073
CA
LYS
A
379
30.444
−10.414
23.850
1.00
25.49
C


ATOM
6075
CB
LYS
A
379
30.133
−9.997
25.295
1.00
26.24
C


ATOM
6078
CG
LYS
A
379
30.943
−8.799
25.790
1.00
30.21
C


ATOM
6081
CD
LYS
A
379
30.509
−8.384
27.194
1.00
37.13
C


ATOM
6084
CE
LYS
A
379
31.545
−7.490
27.876
1.00
41.56
C


ATOM
6087
NZ
LYS
A
379
31.669
−6.147
27.238
1.00
42.65
N


ATOM
6091
C
LYS
A
379
29.768
−11.739
23.493
1.00
24.88
C


ATOM
6092
O
LYS
A
379
29.346
−12.490
24.374
1.00
25.87
O


ATOM
6094
N
SER
A
380
29.684
−12.021
22.193
1.00
23.82
N


ATOM
6095
CA
SER
A
380
29.046
−13.241
21.702
1.00
22.71
C


ATOM
6097
CB
SER
A
380
28.757
−13.146
20.201
1.00
22.21
C


ATOM
6100
OG
SER
A
380
27.753
−12.185
19.935
1.00
23.40
O


ATOM
6102
C
SER
A
380
29.920
−14.455
21.981
1.00
21.70
C


ATOM
6103
O
SER
A
380
31.138
−14.347
22.113
1.00
20.41
O


ATOM
6105
N
THR
A
381
29.276
−15.612
22.069
1.00
21.44
N


ATOM
6106
CA
THR
A
381
29.965
−16.868
22.339
1.00
21.90
C


ATOM
6108
CB
THR
A
381
29.603
−17.402
23.738
1.00
21.79
C


ATOM
6110
OG1
THR
A
381
28.181
−17.514
23.852
1.00
21.72
O


ATOM
6112
CG2
THR
A
381
30.126
−16.456
24.822
1.00
21.88
C


ATOM
6116
C
THR
A
381
29.588
−17.892
21.258
1.00
21.57
C


ATOM
6117
O
THR
A
381
28.876
−18.860
21.539
1.00
21.91
O


ATOM
6119
N
PRO
A
382
30.074
−17.677
20.017
1.00
20.04
N


ATOM
6120
CA
PRO
A
382
29.695
−18.521
18.892
1.00
19.83
C


ATOM
6122
CB
PRO
A
382
30.328
−17.809
17.696
1.00
20.07
C


ATOM
6125
CG
PRO
A
382
31.515
−17.127
18.263
1.00
20.37
C


ATOM
6128
CD
PRO
A
382
31.105
−16.692
19.637
1.00
19.89
C


ATOM
6131
C
PRO
A
382
30.242
−19.938
19.006
1.00
19.76
C


ATOM
6132
O
PRO
A
382
31.224
−20.177
19.719
1.00
20.31
O


ATOM
6133
N
THR
A
383
29.601
−20.864
18.299
1.00
18.95
N


ATOM
6134
CA
THR
A
383
30.034
−22.258
18.273
1.00
18.77
C


ATOM
6136
CB
THR
A
383
28.981
−23.149
17.588
1.00
19.63
C


ATOM
6138
OG1
THR
A
383
28.694
−22.634
16.277
1.00
20.80
O


ATOM
6140
CG2
THR
A
383
27.701
−23.183
18.414
1.00
16.56
C


ATOM
6144
C
THR
A
383
31.351
−22.375
17.520
1.00
18.13
C


ATOM
6145
O
THR
A
383
31.705
−21.475
16.752
1.00
18.02
O


ATOM
6147
N
PHE
A
384
32.075
−23.473
17.745
1.00
17.33
N


ATOM
6148
CA
PHE
A
384
33.358
−23.680
17.077
1.00
16.74
C


ATOM
6150
CB
PHE
A
384
34.002
−25.016
17.462
1.00
16.69
C


ATOM
6153
CG
PHE
A
384
35.303
−25.284
16.727
1.00
18.57
C


ATOM
6154
CD1
PHE
A
384
36.487
−24.662
17.125
1.00
15.55
C


ATOM
6156
CE1
PHE
A
384
37.680
−24.891
16.451
1.00
14.53
C


ATOM
6158
CZ
PHE
A
384
37.700
−25.732
15.364
1.00
14.74
C


ATOM
6160
CE2
PHE
A
384
36.524
−26.347
14.939
1.00
15.29
C


ATOM
6162
CD2
PHE
A
384
35.335
−26.120
15.616
1.00
18.30
C


ATOM
6164
C
PHE
A
384
33.214
−23.615
15.557
1.00
16.96
C


ATOM
6165
O
PHE
A
384
34.044
−23.015
14.872
1.00
16.67
O


ATOM
6167
N
ASP
A
385
32.161
−24.235
15.034
1.00
18.03
N


ATOM
6168
CA
ASP
A
385
31.939
−24.264
13.587
1.00
18.66
C


ATOM
6170
CB
ASP
A
385
30.774
−25.201
13.220
1.00
18.48
C


ATOM
6173
CG
ASP
A
385
31.118
−26.674
13.425
1.00
17.10
C


ATOM
6174
OD1
ASP
A
385
32.317
−27.005
13.495
1.00
20.97
O


ATOM
6175
OD2
ASP
A
385
30.196
−27.509
13.514
1.00
22.63
O


ATOM
6176
C
ASP
A
385
31.727
−22.865
13.006
1.00
19.02
C


ATOM
6177
O
ASP
A
385
32.262
−22.558
11.940
1.00
20.10
O


ATOM
6179
N
ASP
A
386
30.983
−22.013
13.707
1.00
19.37
N


ATOM
6180
CA
ASP
A
386
30.793
−20.627
13.248
1.00
19.83
C


ATOM
6182
CB
ASP
A
386
29.613
−19.953
13.960
1.00
20.91
C


ATOM
6185
CG
ASP
A
386
28.257
−20.503
13.517
1.00
25.38
C


ATOM
6186
OD1
ASP
A
386
28.188
−21.255
12.514
1.00
30.04
O


ATOM
6187
OD2
ASP
A
386
27.252
−20.178
14.182
1.00
34.85
O


ATOM
6188
C
ASP
A
386
32.050
−19.776
13.409
1.00
18.64
C


ATOM
6189
O
ASP
A
386
32.391
−19.021
12.505
1.00
18.66
O


ATOM
6191
N
TYR
A
387
32.738
−19.894
14.544
1.00
18.41
N


ATOM
6192
CA
TYR
A
387
33.961
−19.107
14.777
1.00
18.58
C


ATOM
6194
CB
TYR
A
387
34.470
−19.237
16.214
1.00
18.41
C


ATOM
6197
CG
TYR
A
387
35.731
−18.444
16.486
1.00
20.51
C


ATOM
6198
CD1
TYR
A
387
36.987
−18.991
16.260
1.00
25.70
C


ATOM
6200
CE1
TYR
A
387
38.149
−18.259
16.500
1.00
26.65
C


ATOM
6202
CZ
TYR
A
387
38.057
−16.968
16.986
1.00
29.48
C


ATOM
6203
OH
TYR
A
387
39.197
−16.236
17.235
1.00
32.42
O


ATOM
6205
CE2
TYR
A
387
36.822
−16.405
17.221
1.00
27.61
C


ATOM
6207
CD2
TYR
A
387
35.668
−17.143
16.971
1.00
25.22
C


ATOM
6209
C
TYR
A
387
35.049
−19.558
13.835
1.00
18.22
C


ATOM
6210
O
TYR
A
387
35.601
−18.757
13.087
1.00
17.74
O


ATOM
6212
N
PHE
A
388
35.354
−20.852
13.879
1.00
18.67
N


ATOM
6213
CA
PHE
A
388
36.435
−21.396
13.071
1.00
18.28
C


ATOM
6215
CB
PHE
A
388
36.648
−22.893
13.299
1.00
18.71
C


ATOM
6218
CG
PHE
A
388
37.776
−23.442
12.491
1.00
20.24
C


ATOM
6219
CD1
PHE
A
388
39.088
−23.130
12.821
1.00
16.48
C


ATOM
6221
CE1
PHE
A
388
40.142
−23.605
12.059
1.00
19.99
C


ATOM
6223
CZ
PHE
A
388
39.892
−24.392
10.943
1.00
21.72
C


ATOM
6225
CE2
PHE
A
388
38.583
−24.688
10.590
1.00
24.82
C


ATOM
6227
CD2
PHE
A
388
37.531
−24.210
11.362
1.00
21.91
C


ATOM
6229
C
PHE
A
388
36.189
−21.150
11.604
1.00
16.97
C


ATOM
6230
O
PHE
A
388
37.110
−20.777
10.885
1.00
18.29
O


ATOM
6232
N
GLY
A
389
34.951
−21.356
11.170
1.00
15.96
N


ATOM
6233
CA
GLY
A
389
34.555
−21.080
9.790
1.00
16.08
C


ATOM
6236
C
GLY
A
389
34.930
−19.679
9.338
1.00
15.85
C


ATOM
6237
O
GLY
A
389
35.394
−19.485
8.225
1.00
17.12
O


ATOM
6239
N
ASN
A
390
34.730
−18.702
10.212
1.00
15.50
N


ATOM
6240
CA
ASN
A
390
35.160
−17.337
9.953
1.00
15.29
C


ATOM
6242
CB
ASN
A
390
34.453
−16.386
10.917
1.00
14.58
C


ATOM
6245
CG
ASN
A
390
34.578
−14.942
10.500
1.00
16.26
C


ATOM
6246
OD1
ASN
A
390
33.953
−14.516
9.528
1.00
19.33
O


ATOM
6247
ND2
ASN
A
390
35.388
−14.172
11.234
1.00
11.57
N


ATOM
6250
C
ASN
A
390
36.680
−17.191
10.111
1.00
16.42
C


ATOM
6251
O
ASN
A
390
37.333
−16.463
9.357
1.00
17.12
O


ATOM
6253
N
ALA
A
391
37.231
−17.890
11.102
1.00
16.29
N


ATOM
6254
CA
ALA
A
391
38.632
−17.768
11.460
1.00
16.32
C


ATOM
6256
CB
ALA
A
391
38.935
−18.610
12.685
1.00
16.29
C


ATOM
6260
C
ALA
A
391
39.587
−18.128
10.328
1.00
17.19
C


ATOM
6261
O
ALA
A
391
40.603
−17.460
10.150
1.00
18.59
O


ATOM
6263
N
TRP
A
392
39.296
−19.176
9.564
1.00
17.74
N


ATOM
6264
CA
TRP
A
392
40.227
−19.552
8.498
1.00
17.73
C


ATOM
6266
CB
TRP
A
392
40.053
−21.005
8.024
1.00
18.15
C


ATOM
6269
CG
TRP
A
392
38.758
−21.397
7.371
1.00
18.01
C


ATOM
6270
CD1
TRP
A
392
37.728
−22.100
7.945
1.00
22.06
C


ATOM
6272
NE1
TRP
A
392
36.730
−22.319
7.025
1.00
19.55
N


ATOM
6274
CE2
TRP
A
392
37.110
−21.775
5.827
1.00
15.70
C


ATOM
6275
CD2
TRP
A
392
38.388
−21.197
6.006
1.00
16.64
C


ATOM
6276
CE3
TRP
A
392
39.006
−20.578
4.916
1.00
16.17
C


ATOM
6278
CZ3
TRP
A
392
38.337
−20.554
3.696
1.00
17.82
C


ATOM
6280
CH2
TRP
A
392
37.072
−21.125
3.555
1.00
16.95
C


ATOM
6282
CZ2
TRP
A
392
36.439
−21.736
4.611
1.00
17.70
C


ATOM
6284
C
TRP
A
392
40.175
−18.546
7.358
1.00
17.93
C


ATOM
6285
O
TRP
A
392
41.138
−18.393
6.613
1.00
18.60
O


ATOM
6287
N
LYS
A
393
39.056
−17.842
7.243
1.00
18.79
N


ATOM
6288
CA
LYS
A
393
38.957
−16.741
6.303
1.00
19.11
C


ATOM
6290
CB
LYS
A
393
37.489
−16.404
6.019
1.00
19.71
C


ATOM
6293
CG
LYS
A
393
36.692
−17.548
5.361
1.00
23.76
C


ATOM
6296
CD
LYS
A
393
35.243
−17.111
5.105
1.00
32.08
C


ATOM
6299
CE
LYS
A
393
34.489
−18.013
4.132
1.00
35.08
C


ATOM
6302
NZ
LYS
A
393
33.860
−19.184
4.819
1.00
41.63
N


ATOM
6306
C
LYS
A
393
39.740
−15.526
6.837
1.00
17.96
C


ATOM
6307
O
LYS
A
393
40.536
−14.934
6.105
1.00
17.30
O


ATOM
6309
N
SER
A
394
39.549
−15.191
8.114
1.00
17.21
N


ATOM
6310
CA
SER
A
394
40.243
−14.056
8.727
1.00
17.57
C


ATOM
6312
CB
SER
A
394
39.678
−13.741
10.117
1.00
18.73
C


ATOM
6315
OG
SER
A
394
40.097
−14.679
11.092
1.00
19.38
O


ATOM
6317
C
SER
A
394
41.753
−14.255
8.828
1.00
17.97
C


ATOM
6318
O
SER
A
394
42.482
−13.304
9.097
1.00
18.69
O


ATOM
6320
N
SER
A
395
42.224
−15.483
8.611
1.00
18.04
N


ATOM
6321
CA
SER
A
395
43.663
−15.753
8.549
1.00
17.80
C


ATOM
6323
CB
SER
A
395
43.931
−17.248
8.341
1.00
17.23
C


ATOM
6326
OG
SER
A
395
43.621
−17.657
7.022
1.00
17.01
O


ATOM
6328
C
SER
A
395
44.361
−14.960
7.443
1.00
17.85
C


ATOM
6329
O
SER
A
395
45.549
−14.651
7.568
1.00
17.80
O


ATOM
6331
N
SER
A
396
43.605
−14.637
6.386
1.00
16.98
N


ATOM
6332
CA
SER
A
396
44.119
−14.054
5.137
1.00
17.11
C


ATOM
6334
CB
SER
A
396
45.134
−12.927
5.377
1.00
16.44
C


ATOM
6337
OG
SER
A
396
46.445
−13.431
5.553
1.00
15.62
O


ATOM
6339
C
SER
A
396
44.704
−15.114
4.193
1.00
17.03
C


ATOM
6340
O
SER
A
396
45.119
−14.788
3.080
1.00
16.74
O


ATOM
6342
N
GLY
A
397
44.722
−16.375
4.629
1.00
16.65
N


ATOM
6343
CA
GLY
A
397
45.150
−17.487
3.773
1.00
16.98
C


ATOM
6346
C
GLY
A
397
44.462
−17.567
2.415
1.00
15.97
C


ATOM
6347
O
GLY
A
397
45.121
−17.563
1.381
1.00
16.15
O


ATOM
6349
N
PRO
A
398
43.128
−17.653
2.405
1.00
15.97
N


ATOM
6350
CA
PRO
A
398
42.423
−17.639
1.128
1.00
16.89
C


ATOM
6352
CB
PRO
A
398
40.954
−17.514
1.541
1.00
16.76
C


ATOM
6355
CG
PRO
A
398
40.898
−18.068
2.905
1.00
17.51
C


ATOM
6358
CD
PRO
A
398
42.199
−17.720
3.545
1.00
15.87
C


ATOM
6361
C
PRO
A
398
42.823
−16.455
0.245
1.00
16.96
C


ATOM
6362
O
PRO
A
398
43.060
−16.630
−0.953
1.00
15.67
O


ATOM
6363
N
LEU
A
399
42.907
−15.267
0.845
1.00
17.42
N


ATOM
6364
CA
LEU
A
399
43.167
−14.057
0.081
1.00
18.17
C


ATOM
6366
CB
LEU
A
399
43.085
−12.805
0.956
1.00
18.47
C


ATOM
6369
CG
LEU
A
399
43.337
−11.493
0.198
1.00
18.57
C


ATOM
6371
CD1
LEU
A
399
42.370
−10.407
0.635
1.00
19.49
C


ATOM
6375
CD2
LEU
A
399
44.784
−11.051
0.359
1.00
17.72
C


ATOM
6379
C
LEU
A
399
44.529
−14.156
−0.564
1.00
18.47
C


ATOM
6380
O
LEU
A
399
44.656
−13.968
−1.777
1.00
18.45
O


ATOM
6382
N
GLN
A
400
45.534
−14.468
0.250
1.00
17.29
N


ATOM
6383
CA
GLN
A
400
46.889
−14.653
−0.246
1.00
17.28
C


ATOM
6385
CB
GLN
A
400
47.806
−15.178
0.855
1.00
17.64
C


ATOM
6388
CG
GLN
A
400
48.103
−14.172
1.957
1.00
20.06
C


ATOM
6391
CD
GLN
A
400
49.064
−14.712
2.998
1.00
22.79
C


ATOM
6392
OE1
GLN
A
400
50.063
−15.357
2.662
1.00
26.66
O


ATOM
6393
NE2
GLN
A
400
48.779
−14.440
4.270
1.00
23.20
N


ATOM
6396
C
GLN
A
400
46.911
−15.615
−1.421
1.00
17.50
C


ATOM
6397
O
GLN
A
400
47.584
−15.360
−2.421
1.00
18.78
O


ATOM
6399
N
LEU
A
401
46.164
−16.711
−1.315
1.00
17.11
N


ATOM
6400
CA
LEU
A
401
46.221
−17.751
−2.344
1.00
17.07
C


ATOM
6402
CB
LEU
A
401
45.699
−19.086
−1.815
1.00
16.18
C


ATOM
6405
CG
LEU
A
401
46.625
−19.745
−0.788
1.00
15.41
C


ATOM
6407
CD1
LEU
A
401
46.020
−21.034
−0.275
1.00
15.00
C


ATOM
6411
CD2
LEU
A
401
47.992
−20.002
−1.382
1.00
11.49
C


ATOM
6415
C
LEU
A
401
45.507
−17.337
−3.624
1.00
17.35
C


ATOM
6416
O
LEU
A
401
45.909
−17.744
−4.712
1.00
18.56
O


ATOM
6418
N
VAL
A
402
44.475
−16.510
−3.507
1.00
17.20
N


ATOM
6419
CA
VAL
A
402
43.854
−15.929
−4.694
1.00
17.04
C


ATOM
6421
CB
VAL
A
402
42.638
−15.060
−4.330
1.00
16.83
C


ATOM
6423
CG1
VAL
A
402
42.189
−14.242
−5.526
1.00
16.23
C


ATOM
6427
CG2
VAL
A
402
41.495
−15.938
−3.807
1.00
14.08
C


ATOM
6431
C
VAL
A
402
44.906
−15.107
−5.456
1.00
17.06
C


ATOM
6432
O
VAL
A
402
45.104
−15.282
−6.654
1.00
16.97
O


ATOM
6434
N
PHE
A
403
45.612
−14.246
−4.742
1.00
17.15
N


ATOM
6435
CA
PHE
A
403
46.661
−13.449
−5.352
1.00
18.02
C


ATOM
6437
CB
PHE
A
403
47.166
−12.380
−4.381
1.00
18.40
C


ATOM
6440
CG
PHE
A
403
46.228
−11.211
−4.253
1.00
16.89
C


ATOM
6441
CD1
PHE
A
403
45.130
−11.270
−3.401
1.00
18.92
C


ATOM
6443
CE1
PHE
A
403
44.253
−10.204
−3.292
1.00
18.88
C


ATOM
6445
CZ
PHE
A
403
44.465
−9.067
−4.052
1.00
21.23
C


ATOM
6447
CE2
PHE
A
403
45.556
−9.002
−4.908
1.00
18.67
C


ATOM
6449
CD2
PHE
A
403
46.424
−10.071
−5.004
1.00
15.42
C


ATOM
6451
C
PHE
A
403
47.790
−14.329
−5.868
1.00
18.82
C


ATOM
6452
O
PHE
A
403
48.321
−14.081
−6.954
1.00
19.35
O


ATOM
6454
N
ALA
A
404
48.128
−15.373
−5.114
1.00
18.63
N


ATOM
6455
CA
ALA
A
404
49.131
−16.339
−5.556
1.00
17.59
C


ATOM
6457
CB
ALA
A
404
49.348
−17.400
−4.502
1.00
16.79
C


ATOM
6461
C
ALA
A
404
48.724
−16.984
−6.883
1.00
17.58
C


ATOM
6462
O
ALA
A
404
49.557
−17.128
−7.783
1.00
16.70
O


ATOM
6464
N
TYR
A
405
47.451
−17.373
−6.995
1.00
17.29
N


ATOM
6465
CA
TYR
A
405
46.942
−18.006
−8.218
1.00
17.42
C


ATOM
6467
CB
TYR
A
405
45.422
−18.176
−8.183
1.00
16.99
C


ATOM
6470
CG
TYR
A
405
44.846
−18.697
−9.479
1.00
17.27
C


ATOM
6471
CD1
TYR
A
405
44.809
−20.058
−9.743
1.00
20.07
C


ATOM
6473
CE1
TYR
A
405
44.276
−20.550
−10.932
1.00
22.26
C


ATOM
6475
CZ
TYR
A
405
43.786
−19.668
−11.879
1.00
26.25
C


ATOM
6476
OH
TYR
A
405
43.268
−20.156
−13.067
1.00
25.57
O


ATOM
6478
CE2
TYR
A
405
43.823
−18.301
−11.637
1.00
20.59
C


ATOM
6480
CD2
TYR
A
405
44.353
−17.829
−10.445
1.00
18.93
C


ATOM
6482
C
TYR
A
405
47.298
−17.204
−9.452
1.00
17.58
C


ATOM
6483
O
TYR
A
405
47.773
−17.760
−10.434
1.00
18.44
O


ATOM
6485
N
PHE
A
406
47.068
−15.898
−9.401
1.00
18.39
N


ATOM
6486
CA
PHE
A
406
47.265
−15.059
−10.577
1.00
18.51
C


ATOM
6488
CB
PHE
A
406
46.485
−13.752
−10.446
1.00
18.24
C


ATOM
6491
CG
PHE
A
406
45.002
−13.943
−10.486
1.00
17.37
C


ATOM
6492
CD1
PHE
A
406
44.367
−14.241
−11.681
1.00
17.72
C


ATOM
6494
CE1
PHE
A
406
43.006
−14.428
−11.722
1.00
16.27
C


ATOM
6496
CZ
PHE
A
406
42.257
−14.318
−10.563
1.00
16.45
C


ATOM
6498
CE2
PHE
A
406
42.874
−14.023
−9.371
1.00
13.88
C


ATOM
6500
CD2
PHE
A
406
44.241
−13.840
−9.335
1.00
16.63
C


ATOM
6502
C
PHE
A
406
48.732
−14.795
−10.858
1.00
18.16
C


ATOM
6503
O
PHE
A
406
49.090
−14.506
−11.985
1.00
19.24
O


ATOM
6505
N
ALA
A
407
49.582
−14.896
−9.848
1.00
18.51
N


ATOM
6506
CA
ALA
A
407
51.014
−14.685
−10.050
1.00
20.28
C


ATOM
6508
CB
ALA
A
407
51.653
−14.150
−8.781
1.00
19.88
C


ATOM
6512
C
ALA
A
407
51.730
−15.956
−10.501
1.00
21.89
C


ATOM
6513
O
ALA
A
407
52.816
−15.882
−11.057
1.00
21.80
O


ATOM
6515
N
VAL
A
408
51.121
−17.112
−10.249
1.00
24.28
N


ATOM
6516
CA
VAL
A
408
51.728
−18.414
−10.543
1.00
25.70
C


ATOM
6518
CB
VAL
A
408
51.447
−19.400
−9.410
1.00
25.52
C


ATOM
6520
CG1
VAL
A
408
51.696
−20.832
−9.863
1.00
25.95
C


ATOM
6524
CG2
VAL
A
408
52.290
−19.057
−8.202
1.00
26.22
C


ATOM
6528
C
VAL
A
408
51.198
−19.018
−11.842
1.00
28.31
C


ATOM
6529
O
VAL
A
408
51.957
−19.568
−12.631
1.00
27.49
O


ATOM
6531
N
VAL
A
409
49.892
−18.924
−12.057
1.00
31.68
N


ATOM
6532
CA
VAL
A
409
49.278
−19.504
−13.244
1.00
34.10
C


ATOM
6534
CB
VAL
A
409
47.745
−19.504
−13.133
1.00
33.82
C


ATOM
6536
CG1
VAL
A
409
47.106
−19.782
−14.466
1.00
34.76
C


ATOM
6540
CG2
VAL
A
409
47.311
−20.543
−12.121
1.00
35.18
C


ATOM
6544
C
VAL
A
409
49.706
−18.767
−14.508
1.00
36.79
C


ATOM
6545
O
VAL
A
409
49.712
−17.538
−14.549
1.00
37.53
O


ATOM
6547
N
GLN
A
410
50.071
−19.534
−15.532
1.00
39.83
N


ATOM
6548
CA
GLN
A
410
50.398
−18.979
−16.837
1.00
41.91
C


ATOM
6550
CB
GLN
A
410
51.194
−19.996
−17.665
1.00
43.77
C


ATOM
6553
CG
GLN
A
410
51.931
−19.387
−18.876
1.00
49.79
C


ATOM
6556
CD
GLN
A
410
52.277
−20.418
−19.963
1.00
56.08
C


ATOM
6557
OE1
GLN
A
410
52.241
−21.633
−19.735
1.00
58.83
O


ATOM
6558
NE2
GLN
A
410
52.611
−19.926
−21.153
1.00
55.44
N


ATOM
6561
C
GLN
A
410
49.104
−18.585
−17.558
1.00
40.93
C


ATOM
6562
O
GLN
A
410
48.940
−17.425
−17.951
1.00
41.41
O


ATOM
6564
N
ASN
A
411
48.187
−19.547
−17.706
1.00
39.04
N


ATOM
6565
CA
ASN
A
411
46.905
−19.324
−18.387
1.00
37.73
C


ATOM
6567
CB
ASN
A
411
46.731
−20.320
−19.532
1.00
37.58
C


ATOM
6570
CG
ASN
A
411
47.746
−20.125
−20.630
1.00
37.46
C


ATOM
6571
OD1
ASN
A
411
47.896
−19.025
−21.164
1.00
36.26
O


ATOM
6572
ND2
ASN
A
411
48.449
−21.194
−20.982
1.00
38.10
N


ATOM
6575
C
ASN
A
411
45.718
−19.455
−17.448
1.00
36.52
C


ATOM
6576
O
ASN
A
411
45.467
−20.528
−16.909
1.00
36.35
O


ATOM
6578
N
ILE
A
412
44.971
−18.369
−17.285
1.00
36.03
N


ATOM
6579
CA
ILE
A
412
43.825
−18.346
−16.380
1.00
36.69
C


ATOM
6581
CB
ILE
A
412
43.282
−16.918
−16.200
1.00
37.17
C


ATOM
6583
CG1
ILE
A
412
44.380
−15.961
−15.723
1.00
39.66
C


ATOM
6586
CD1
ILE
A
412
43.975
−14.491
−15.792
1.00
39.53
C


ATOM
6590
CG2
ILE
A
412
42.139
−16.905
−15.201
1.00
40.15
C


ATOM
6594
C
ILE
A
412
42.697
−19.226
−16.925
1.00
36.46
C


ATOM
6595
O
ILE
A
412
42.558
−19.374
−18.134
1.00
36.73
O


ATOM
6597
N
LYS
A
413
41.897
−19.805
−16.032
1.00
36.40
N


ATOM
6598
CA
LYS
A
413
40.749
−20.622
−16.434
1.00
36.79
C


ATOM
6600
CB
LYS
A
413
41.087
−22.102
−16.292
1.00
37.04
C


ATOM
6603
CG
LYS
A
413
42.257
−22.533
−17.149
1.00
41.24
C


ATOM
6606
CD
LYS
A
413
42.495
−24.027
−17.053
1.00
46.37
C


ATOM
6609
CE
LYS
A
413
43.488
−24.497
−18.102
1.00
47.62
C


ATOM
6612
NZ
LYS
A
413
43.623
−25.980
−18.089
1.00
49.22
N


ATOM
6616
C
LYS
A
413
39.488
−20.289
−15.628
1.00
36.33
C


ATOM
6617
O
LYS
A
413
39.522
−20.241
−14.394
1.00
35.00
O


ATOM
6619
N
LYS
A
414
38.381
−20.071
−16.343
1.00
36.30
N


ATOM
6620
CA
LYS
A
414
37.108
−19.665
−15.735
1.00
36.02
C


ATOM
6622
CB
LYS
A
414
35.979
−19.665
−16.779
1.00
36.18
C


ATOM
6625
CG
LYS
A
414
34.622
−19.190
−16.222
1.00
41.37
C


ATOM
6628
CD
LYS
A
414
33.688
−18.591
−17.298
1.00
45.90
C


ATOM
6631
CE
LYS
A
414
33.104
−19.652
−18.237
1.00
47.59
C


ATOM
6634
NZ
LYS
A
414
32.125
−20.563
−17.572
1.00
47.85
N


ATOM
6638
C
LYS
A
414
36.723
−20.559
−14.557
1.00
35.11
C


ATOM
6639
O
LYS
A
414
36.341
−20.066
−13.493
1.00
35.15
O


ATOM
6641
N
GLU
A
415
36.834
−21.871
−14.749
1.00
33.73
N


ATOM
6642
CA
GLU
A
415
36.429
−22.835
−13.724
1.00
32.94
C


ATOM
6644
CB
GLU
A
415
36.382
−24.253
−14.310
1.00
33.28
C


ATOM
6647
CG
GLU
A
415
36.060
−25.340
−13.285
1.00
38.04
C


ATOM
6650
CD
GLU
A
415
35.859
−26.706
−13.906
1.00
43.41
C


ATOM
6651
OE1
GLU
A
415
36.344
−26.923
−15.037
1.00
47.72
O


ATOM
6652
OE2
GLU
A
415
35.220
−27.564
−13.257
1.00
45.87
O


ATOM
6653
C
GLU
A
415
37.340
−22.785
−12.486
1.00
30.55
C


ATOM
6654
O
GLU
A
415
36.875
−22.938
−11.357
1.00
29.03
O


ATOM
6656
N
GLU
A
416
38.633
−22.575
−12.699
1.00
29.00
N


ATOM
6657
CA
GLU
A
416
39.565
−22.459
−11.585
1.00
28.18
C


ATOM
6659
CB
GLU
A
416
41.005
−22.381
−12.093
1.00
28.41
C


ATOM
6662
CG
GLU
A
416
41.504
−23.662
−12.739
1.00
29.21
C


ATOM
6665
CD
GLU
A
416
42.948
−23.576
−13.186
1.00
31.23
C


ATOM
6666
OE1
GLU
A
416
43.381
−22.515
−13.681
1.00
37.41
O


ATOM
6667
OE2
GLU
A
416
43.658
−24.583
−13.049
1.00
39.19
O


ATOM
6668
C
GLU
A
416
39.229
−21.232
−10.736
1.00
27.53
C


ATOM
6669
O
GLU
A
416
39.164
−21.326
−9.515
1.00
25.71
O


ATOM
6671
N
ILE
A
417
39.000
−20.090
−11.383
1.00
28.17
N


ATOM
6672
CA
ILE
A
417
38.673
−18.869
−10.644
1.00
29.32
C


ATOM
6674
CB
ILE
A
417
38.889
−17.556
−11.477
1.00
29.41
C


ATOM
6676
CG1
ILE
A
417
37.945
−17.448
−12.661
1.00
33.17
C


ATOM
6679
CD1
ILE
A
417
38.232
−16.225
−13.522
1.00
37.63
C


ATOM
6683
CG2
ILE
A
417
40.313
−17.469
−11.992
1.00
30.67
C


ATOM
6687
C
ILE
A
417
37.270
−18.938
−10.019
1.00
28.55
C


ATOM
6688
O
ILE
A
417
37.065
−18.456
−8.908
1.00
28.52
O


ATOM
6690
N
GLU
A
418
36.320
−19.558
−10.710
1.00
28.77
N


ATOM
6691
CA
GLU
A
418
35.000
−19.811
−10.118
1.00
29.37
C


ATOM
6693
CB
GLU
A
418
34.084
−20.562
−11.091
1.00
29.70
C


ATOM
6696
CG
GLU
A
418
33.386
−19.642
−12.103
1.00
34.62
C


ATOM
6699
CD
GLU
A
418
32.662
−20.391
−13.231
1.00
39.25
C


ATOM
6700
OE1
GLU
A
418
32.662
−21.644
−13.247
1.00
37.70
O


ATOM
6701
OE2
GLU
A
418
32.092
−19.711
−14.110
1.00
40.61
O


ATOM
6702
C
GLU
A
418
35.146
−20.589
−8.816
1.00
28.68
C


ATOM
6703
O
GLU
A
418
34.510
−20.265
−7.816
1.00
29.24
O


ATOM
6705
N
ASN
A
419
36.011
−21.595
−8.823
1.00
28.36
N


ATOM
6706
CA
ASN
A
419
36.233
−22.414
−7.635
1.00
27.94
C


ATOM
6708
CB
ASN
A
419
36.925
−23.729
−8.008
1.00
27.24
C


ATOM
6711
CG
ASN
A
419
35.945
−24.773
−8.496
1.00
28.86
C


ATOM
6712
OD1
ASN
A
419
35.470
−25.609
−7.725
1.00
33.67
O


ATOM
6713
ND2
ASN
A
419
35.611
−24.712
−9.771
1.00
30.80
N


ATOM
6716
C
ASN
A
419
36.987
−21.680
−6.529
1.00
28.15
C


ATOM
6717
O
ASN
A
419
36.761
−21.941
−5.344
1.00
27.94
O


ATOM
6719
N
LEU
A
420
37.868
−20.756
−6.913
1.00
28.22
N


ATOM
6720
CA
LEU
A
420
38.505
−19.866
−5.945
1.00
28.08
C


ATOM
6722
CB
LEU
A
420
39.486
−18.906
−6.632
1.00
27.57
C


ATOM
6725
CG
LEU
A
420
40.804
−19.498
−7.142
1.00
26.93
C


ATOM
6727
CD1
LEU
A
420
41.615
−18.442
−7.883
1.00
21.67
C


ATOM
6731
CD2
LEU
A
420
41.609
−20.119
−6.004
1.00
22.68
C


ATOM
6735
C
LEU
A
420
37.454
−19.068
−5.162
1.00
29.46
C


ATOM
6736
O
LEU
A
420
37.514
−18.996
−3.936
1.00
29.59
O


ATOM
6738
N
GLN
A
421
36.489
−18.485
−5.871
1.00
31.06
N


ATOM
6739
CA
GLN
A
421
35.430
−17.688
−5.233
1.00
32.60
C


ATOM
6741
CB
GLN
A
421
34.538
−17.030
−6.286
1.00
33.15
C


ATOM
6744
CG
GLN
A
421
35.190
−15.880
−6.998
1.00
36.48
C


ATOM
6747
CD
GLN
A
421
34.181
−14.962
−7.639
1.00
41.39
C


ATOM
6748
OE1
GLN
A
421
34.099
−13.778
−7.300
1.00
46.29
O


ATOM
6749
NE2
GLN
A
421
33.395
−15.502
−8.569
1.00
43.36
N


ATOM
6752
C
GLN
A
421
34.543
−18.486
−4.276
1.00
32.84
C


ATOM
6753
O
GLN
A
421
34.009
−17.923
−3.320
1.00
32.60
O


ATOM
6755
N
LYS
A
422
34.380
−19.783
−4.552
1.00
33.10
N


ATOM
6756
CA
LYS
A
422
33.589
−20.684
−3.704
1.00
33.01
C


ATOM
6758
CB
LYS
A
422
33.026
−21.847
−4.533
1.00
33.85
C


ATOM
6761
CG
LYS
A
422
32.067
−21.450
−5.650
1.00
37.74
C


ATOM
6764
CD
LYS
A
422
31.792
−22.637
−6.576
1.00
42.89
C


ATOM
6767
CE
LYS
A
422
30.949
−22.241
−7.791
1.00
46.16
C


ATOM
6770
NZ
LYS
A
422
30.975
−23.307
−8.845
1.00
44.93
N


ATOM
6774
C
LYS
A
422
34.418
−21.263
−2.559
1.00
31.85
C


ATOM
6775
O
LYS
A
422
33.918
−22.077
−1.792
1.00
31.74
O


ATOM
6777
N
TYR
A
423
35.679
−20.850
−2.457
1.00
31.54
N


ATOM
6778
CA
TYR
A
423
36.625
−21.387
−1.473
1.00
31.88
C


ATOM
6780
CB
TYR
A
423
36.243
−20.978
−0.049
1.00
32.14
C


ATOM
6783
CG
TYR
A
423
36.306
−19.488
0.108
1.00
34.99
C


ATOM
6784
CD1
TYR
A
423
37.532
−18.844
0.243
1.00
38.45
C


ATOM
6786
CE1
TYR
A
423
37.610
−17.463
0.359
1.00
39.93
C


ATOM
6788
CZ
TYR
A
423
36.459
−16.711
0.333
1.00
41.43
C


ATOM
6789
OH
TYR
A
423
36.566
−15.347
0.450
1.00
46.46
O


ATOM
6791
CE2
TYR
A
423
35.222
−17.320
0.183
1.00
40.38
C


ATOM
6793
CD2
TYR
A
423
35.154
−18.709
0.064
1.00
39.66
C


ATOM
6795
C
TYR
A
423
36.804
−22.888
−1.619
1.00
30.75
C


ATOM
6796
O
TYR
A
423
36.601
−23.664
−0.683
1.00
31.19
O


ATOM
6798
N
HIS
A
424
37.202
−23.280
−2.820
1.00
29.71
N


ATOM
6799
CA
HIS
A
424
37.529
−24.659
−3.095
1.00
29.55
C


ATOM
6801
CB
HIS
A
424
38.046
−24.811
−4.528
1.00
29.55
C


ATOM
6804
CG
HIS
A
424
38.156
−26.232
−4.983
1.00
33.09
C


ATOM
6805
ND1
HIS
A
424
39.369
−26.863
−5.169
1.00
33.16
N


ATOM
6807
CE1
HIS
A
424
39.157
−28.105
−5.570
1.00
35.89
C


ATOM
6809
NE2
HIS
A
424
37.851
−28.303
−5.642
1.00
36.09
N


ATOM
6811
CD2
HIS
A
424
37.202
−27.148
−5.278
1.00
33.63
C


ATOM
6813
C
HIS
A
424
38.575
−25.129
−2.093
1.00
28.60
C


ATOM
6814
O
HIS
A
424
39.387
−24.339
−1.611
1.00
28.26
O


ATOM
6816
N
ASP
A
425
38.543
−26.423
−1.793
1.00
28.17
N


ATOM
6817
CA
ASP
A
425
39.435
−27.031
−0.802
1.00
27.44
C


ATOM
6819
CB
ASP
A
425
39.238
−28.546
−0.794
1.00
27.96
C


ATOM
6822
CG
ASP
A
425
37.854
−28.954
−0.302
1.00
30.64
C


ATOM
6823
OD1
ASP
A
425
37.346
−28.327
0.658
1.00
34.80
O


ATOM
6824
OD2
ASP
A
425
37.283
−29.909
−0.874
1.00
34.42
O


ATOM
6825
C
ASP
A
425
40.920
−26.710
−0.994
1.00
25.25
C


ATOM
6826
O
ASP
A
425
41.666
−26.613
−0.022
1.00
25.57
O


ATOM
6828
N
THR
A
426
41.339
−26.549
−2.243
1.00
23.30
N


ATOM
6829
CA
THR
A
426
42.724
−26.225
−2.564
1.00
22.46
C


ATOM
6831
CB
THR
A
426
42.870
−25.912
−4.049
1.00
22.03
C


ATOM
6833
OG1
THR
A
426
42.320
−26.994
−4.802
1.00
28.59
O


ATOM
6835
CG2
THR
A
426
44.310
−25.747
−4.418
1.00
20.27
C


ATOM
6839
C
THR
A
426
43.250
−25.044
−1.762
1.00
21.80
C


ATOM
6840
O
THR
A
426
44.406
−25.052
−1.343
1.00
21.28
O


ATOM
6842
N
ILE
A
427
42.407
−24.037
−1.548
1.00
21.03
N


ATOM
6843
CA
ILE
A
427
42.804
−22.885
−0.753
1.00
21.51
C


ATOM
6845
CB
ILE
A
427
42.540
−21.548
−1.487
1.00
20.95
C


ATOM
6847
CG1
ILE
A
427
41.059
−21.215
−1.544
1.00
20.68
C


ATOM
6850
CD1
ILE
A
427
40.795
−19.868
−2.154
1.00
20.51
C


ATOM
6854
CG2
ILE
A
427
43.114
−21.592
−2.883
1.00
20.86
C


ATOM
6858
C
ILE
A
427
42.186
−22.849
0.652
1.00
22.31
C


ATOM
6859
O
ILE
A
427
42.747
−22.224
1.551
1.00
23.88
O


ATOM
6861
N
SER
A
428
41.054
−23.511
0.866
1.00
21.82
N


ATOM
6862
CA
SER
A
428
40.474
−23.516
2.206
1.00
22.33
C


ATOM
6864
CB
SER
A
428
39.028
−24.017
2.198
1.00
21.31
C


ATOM
6867
OG
SER
A
428
38.972
−25.370
1.821
1.00
24.55
O


ATOM
6869
C
SER
A
428
41.343
−24.344
3.157
1.00
22.40
C


ATOM
6870
O
SER
A
428
41.712
−23.879
4.229
1.00
22.34
O


ATOM
6872
N
ARG
A
429
41.707
−25.550
2.742
1.00
22.23
N


ATOM
6873
CA
ARG
A
429
42.416
−26.464
3.628
1.00
22.93
C


ATOM
6875
CB
ARG
A
429
42.585
−27.832
2.964
1.00
23.58
C


ATOM
6878
CG
ARG
A
429
41.284
−28.562
2.862
1.00
27.96
C


ATOM
6881
CD
ARG
A
429
41.461
−29.989
2.472
1.00
36.79
C


ATOM
6884
NE
ARG
A
429
40.155
−30.586
2.201
1.00
43.35
N


ATOM
6886
CZ
ARG
A
429
39.813
−31.223
1.082
1.00
44.66
C


ATOM
6887
NH1
ARG
A
429
40.679
−31.385
0.080
1.00
43.44
N


ATOM
6890
NH2
ARG
A
429
38.584
−31.708
0.970
1.00
46.29
N


ATOM
6893
C
ARG
A
429
43.752
−25.950
4.180
1.00
22.07
C


ATOM
6894
O
ARG
A
429
43.982
−26.054
5.370
1.00
22.38
O


ATOM
6896
N
PRO
A
430
44.633
−25.399
3.329
1.00
21.78
N


ATOM
6897
CA
PRO
A
430
45.868
−24.818
3.863
1.00
21.02
C


ATOM
6899
CB
PRO
A
430
46.559
−24.243
2.626
1.00
20.87
C


ATOM
6902
CG
PRO
A
430
46.004
−24.985
1.491
1.00
23.01
C


ATOM
6905
CD
PRO
A
430
44.593
−25.339
1.860
1.00
22.62
C


ATOM
6908
C
PRO
A
430
45.599
−23.695
4.840
1.00
20.48
C


ATOM
6909
O
PRO
A
430
46.359
−23.497
5.788
1.00
20.34
O


ATOM
6910
N
SER
A
431
44.518
−22.963
4.603
1.00
19.97
N


ATOM
6911
CA
SER
A
431
44.127
−21.885
5.490
1.00
19.52
C


ATOM
6913
CB
SER
A
431
43.082
−21.004
4.815
1.00
20.32
C


ATOM
6916
OG
SER
A
431
43.532
−20.607
3.532
1.00
19.38
O


ATOM
6918
C
SER
A
431
43.605
−22.428
6.817
1.00
18.44
C


ATOM
6919
O
SER
A
431
43.778
−21.793
7.845
1.00
18.95
O


ATOM
6921
N
HIS
A
432
42.972
−23.596
6.799
1.00
17.54
N


ATOM
6922
CA
HIS
A
432
42.640
−24.289
8.041
1.00
18.72
C


ATOM
6924
CB
HIS
A
432
42.035
−25.679
7.782
1.00
19.51
C


ATOM
6927
CG
HIS
A
432
40.631
−25.662
7.268
1.00
21.68
C


ATOM
6928
ND1
HIS
A
432
40.151
−24.683
6.425
1.00
30.18
N


ATOM
6930
CE1
HIS
A
432
38.889
−24.938
6.129
1.00
29.72
C


ATOM
6932
NE2
HIS
A
432
38.538
−26.058
6.733
1.00
27.31
N


ATOM
6934
CD2
HIS
A
432
39.612
−26.535
7.445
1.00
26.70
C


ATOM
6936
C
HIS
A
432
43.920
−24.471
8.864
1.00
18.63
C


ATOM
6937
O
HIS
A
432
43.962
−24.117
10.043
1.00
17.64
O


ATOM
6939
N
ILE
A
433
44.957
−25.029
8.235
1.00
18.12
N


ATOM
6940
CA
ILE
A
433
46.215
−25.307
8.929
1.00
18.72
C


ATOM
6942
CB
ILE
A
433
47.280
−25.963
8.016
1.00
18.77
C


ATOM
6944
CG1
ILE
A
433
46.783
−27.280
7.409
1.00
21.09
C


ATOM
6947
CD1
ILE
A
433
46.524
−28.349
8.411
1.00
23.35
C


ATOM
6951
CG2
ILE
A
433
48.565
−26.224
8.796
1.00
16.10
C


ATOM
6955
C
ILE
A
433
46.793
−24.004
9.473
1.00
18.88
C


ATOM
6956
O
ILE
A
433
47.226
−23.941
10.617
1.00
18.88
O


ATOM
6958
N
PHE
A
434
46.780
−22.975
8.635
1.00
18.66
N


ATOM
6959
CA
PHE
A
434
47.313
−21.666
8.968
1.00
18.88
C


ATOM
6961
CB
PHE
A
434
47.073
−20.731
7.769
1.00
19.86
C


ATOM
6964
CG
PHE
A
434
47.579
−19.312
7.934
1.00
22.12
C


ATOM
6965
CD1
PHE
A
434
48.493
−18.947
8.924
1.00
23.34
C


ATOM
6967
CE1
PHE
A
434
48.940
−17.627
9.027
1.00
23.55
C


ATOM
6969
CZ
PHE
A
434
48.494
−16.667
8.126
1.00
21.56
C


ATOM
6971
CE2
PHE
A
434
47.601
−17.025
7.127
1.00
25.55
C


ATOM
6973
CD2
PHE
A
434
47.153
−18.337
7.034
1.00
24.16
C


ATOM
6975
C
PHE
A
434
46.677
−21.149
10.253
1.00
18.81
C


ATOM
6976
O
PHE
A
434
47.370
−20.896
11.240
1.00
19.27
O


ATOM
6978
N
ARG
A
435
45.357
−21.023
10.257
1.00
18.71
N


ATOM
6979
CA
ARG
A
435
44.650
−20.554
11.450
1.00
18.49
C


ATOM
6981
CB
ARG
A
435
43.149
−20.424
11.169
1.00
18.78
C


ATOM
6984
CG
ARG
A
435
42.265
−20.203
12.401
1.00
20.05
C


ATOM
6987
CD
ARG
A
435
42.636
−18.948
13.173
1.00
20.50
C


ATOM
6990
NE
ARG
A
435
42.392
−17.736
12.398
1.00
20.69
N


ATOM
6992
CZ
ARG
A
435
42.866
−16.531
12.710
1.00
18.20
C


ATOM
6993
NH1
ARG
A
435
43.628
−16.354
13.783
1.00
16.34
N


ATOM
6996
NH2
ARG
A
435
42.590
−15.499
11.930
1.00
18.57
N


ATOM
6999
C
ARG
A
435
44.897
−21.441
12.679
1.00
18.35
C


ATOM
7000
O
ARG
A
435
45.029
−20.930
13.783
1.00
19.10
O


ATOM
7002
N
LEU
A
436
44.965
−22.757
12.497
1.00
18.51
N


ATOM
7003
CA
LEU
A
436
45.078
−23.670
13.638
1.00
18.31
C


ATOM
7005
CB
LEU
A
436
44.722
−25.106
13.244
1.00
18.73
C


ATOM
7008
CG
LEU
A
436
43.249
−25.412
12.891
1.00
18.89
C


ATOM
7010
CD1
LEU
A
436
43.103
−26.835
12.324
1.00
13.86
C


ATOM
7014
CD2
LEU
A
436
42.326
−25.223
14.081
1.00
11.25
C


ATOM
7018
C
LEU
A
436
46.473
−23.610
14.244
1.00
18.76
C


ATOM
7019
O
LEU
A
436
46.618
−23.631
15.461
1.00
19.39
O


ATOM
7021
N
CYS
A
437
47.493
−23.527
13.395
1.00
19.06
N


ATOM
7022
CA
CYS
A
437
48.869
−23.352
13.846
1.00
19.36
C


ATOM
7024
CB
CYS
A
437
49.831
−23.321
12.655
1.00
19.50
C


ATOM
7027
SG
CYS
A
437
50.151
−24.940
11.899
1.00
22.20
S


ATOM
7029
C
CYS
A
437
49.005
−22.063
14.651
1.00
20.85
C


ATOM
7030
O
CYS
A
437
49.531
−22.070
15.767
1.00
20.54
O


ATOM
7032
N
ASN
A
438
48.526
−20.959
14.080
1.00
22.04
N


ATOM
7033
CA
ASN
A
438
48.545
−19.675
14.761
1.00
22.16
C


ATOM
7035
CB
ASN
A
438
47.798
−18.620
13.940
1.00
23.23
C


ATOM
7038
CG
ASN
A
438
47.863
−17.218
14.556
1.00
23.29
C


ATOM
7039
OD1
ASN
A
438
47.842
−17.043
15.776
1.00
29.89
O


ATOM
7040
ND2
ASN
A
438
47.920
−16.215
13.700
1.00
23.81
N


ATOM
7043
C
ASN
A
438
47.922
−19.803
16.134
1.00
22.70
C


ATOM
7044
O
ASN
A
438
48.567
−19.522
17.145
1.00
24.60
O


ATOM
7046
N
ASP
A
439
46.670
−20.238
16.170
1.00
22.82
N


ATOM
7047
CA
ASP
A
439
45.913
−20.274
17.422
1.00
22.80
C


ATOM
7049
CB
ASP
A
439
44.447
−20.613
17.151
1.00
22.49
C


ATOM
7052
CG
ASP
A
439
43.692
−19.457
16.523
1.00
22.19
C


ATOM
7053
OD1
ASP
A
439
44.344
−18.481
16.083
1.00
21.84
O


ATOM
7054
OD2
ASP
A
439
42.445
−19.526
16.474
1.00
23.84
O


ATOM
7055
C
ASP
A
439
46.518
−21.231
18.445
1.00
22.94
C


ATOM
7056
O
ASP
A
439
46.460
−20.977
19.652
1.00
22.06
O


ATOM
7058
N
LEU
A
440
47.107
−22.320
17.960
1.00
23.94
N


ATOM
7059
CA
LEU
A
440
47.855
−23.236
18.828
1.00
25.10
C


ATOM
7061
CB
LEU
A
440
48.435
−24.403
18.026
1.00
24.61
C


ATOM
7064
CG
LEU
A
440
47.518
−25.600
17.819
1.00
24.36
C


ATOM
7066
CD1
LEU
A
440
48.216
−26.596
16.916
1.00
25.39
C


ATOM
7070
CD2
LEU
A
440
47.145
−26.231
19.152
1.00
21.29
C


ATOM
7074
C
LEU
A
440
48.994
−22.531
19.556
1.00
25.35
C


ATOM
7075
O
LEU
A
440
49.214
−22.764
20.741
1.00
26.43
O


ATOM
7077
N
ALA
A
441
49.712
−21.681
18.831
1.00
25.10
N


ATOM
7078
CA
ALA
A
441
50.892
−21.009
19.355
1.00
24.74
C


ATOM
7080
CB
ALA
A
441
51.572
−20.207
18.249
1.00
24.15
C


ATOM
7084
C
ALA
A
441
50.548
−20.106
20.535
1.00
25.07
C


ATOM
7085
O
ALA
A
441
51.312
−20.034
21.495
1.00
24.89
O


ATOM
7087
N
SER
A
442
49.393
−19.443
20.469
1.00
25.14
N


ATOM
7088
CA
SER
A
442
48.966
−18.510
21.516
1.00
25.55
C


ATOM
7090
CB
SER
A
442
48.481
−17.209
20.882
1.00
25.07
C


ATOM
7093
OG
SER
A
442
47.508
−17.474
19.897
1.00
27.87
O


ATOM
7095
C
SER
A
442
47.879
−19.066
22.448
1.00
26.22
C


ATOM
7096
O
SER
A
442
47.468
−18.392
23.398
1.00
25.43
O


ATOM
7098
N
ALA
A
443
47.436
−20.296
22.194
1.00
26.79
N


ATOM
7099
CA
ALA
A
443
46.314
−20.873
22.929
1.00
27.22
C


ATOM
7101
CB
ALA
A
443
46.131
−22.334
22.556
1.00
27.55
C


ATOM
7105
C
ALA
A
443
46.474
−20.740
24.435
1.00
28.37
C


ATOM
7106
O
ALA
A
443
45.653
−20.104
25.092
1.00
28.82
O


ATOM
7108
N
SER
A
444
47.537
−21.325
24.979
1.00
29.79
N


ATOM
7109
CA
SER
A
444
47.677
−21.439
26.437
1.00
30.42
C


ATOM
7111
CB
SER
A
444
48.835
−22.377
26.819
1.00
30.42
C


ATOM
7114
OG
SER
A
444
50.079
−21.704
26.838
1.00
35.09
O


ATOM
7116
C
SER
A
444
47.825
−20.082
27.120
1.00
30.19
C


ATOM
7117
O
SER
A
444
47.308
−19.884
28.215
1.00
30.28
O


ATOM
7119
N
ALA
A
445
48.520
−19.153
26.468
1.00
31.06
N


ATOM
7120
CA
ALA
A
445
48.665
−17.792
26.984
1.00
31.76
C


ATOM
7122
CB
ALA
A
445
49.669
−17.000
26.149
1.00
31.08
C


ATOM
7126
C
ALA
A
445
47.316
−17.078
27.011
1.00
32.95
C


ATOM
7127
O
ALA
A
445
46.928
−16.517
28.039
1.00
34.62
O


ATOM
7129
N
GLU
A
446
46.602
−17.107
25.887
1.00
33.61
N


ATOM
7130
CA
GLU
A
446
45.315
−16.414
25.766
1.00
34.10
C


ATOM
7132
CB
GLU
A
446
44.805
−16.438
24.314
1.00
34.56
C


ATOM
7135
CG
GLU
A
446
45.647
−15.607
23.327
1.00
37.05
C


ATOM
7138
CD
GLU
A
446
45.093
−15.604
21.898
1.00
39.71
C


ATOM
7139
OE1
GLU
A
446
43.878
−15.817
21.707
1.00
44.12
O


ATOM
7140
OE2
GLU
A
446
45.875
−15.376
20.956
1.00
42.43
O


ATOM
7141
C
GLU
A
446
44.269
−17.003
26.713
1.00
33.79
C


ATOM
7142
O
GLU
A
446
43.495
−16.263
27.316
1.00
33.16
O


ATOM
7144
N
ILE
A
447
44.263
−18.330
26.844
1.00
34.58
N


ATOM
7145
CA
ILE
A
447
43.341
−19.029
27.749
1.00
35.30
C


ATOM
7147
CB
ILE
A
447
43.423
−20.565
27.570
1.00
35.29
C


ATOM
7149
CG1
ILE
A
447
42.832
−20.984
26.221
1.00
36.11
C


ATOM
7152
CD1
ILE
A
447
43.148
−22.424
25.839
1.00
34.34
C


ATOM
7156
CG2
ILE
A
447
42.672
−21.285
28.682
1.00
33.95
C


ATOM
7160
C
ILE
A
447
43.618
−18.678
29.211
1.00
36.55
C


ATOM
7161
O
ILE
A
447
42.689
−18.587
30.017
1.00
36.36
O


ATOM
7163
N
ALA
A
448
44.895
−18.485
29.541
1.00
38.20
N


ATOM
7164
CA
ALA
A
448
45.306
−18.044
30.877
1.00
39.13
C


ATOM
7166
CB
ALA
A
448
46.829
−18.057
30.997
1.00
38.88
C


ATOM
7170
C
ALA
A
448
44.753
−16.651
31.198
1.00
40.09
C


ATOM
7171
O
ALA
A
448
44.246
−16.431
32.299
1.00
40.94
O


ATOM
7173
N
ARG
A
449
44.837
−15.727
30.235
1.00
40.74
N


ATOM
7174
CA
ARG
A
449
44.231
−14.389
30.370
1.00
41.49
C


ATOM
7176
CB
ARG
A
449
44.747
−13.427
29.287
1.00
42.44
C


ATOM
7179
CG
ARG
A
449
46.261
−13.208
29.228
1.00
45.54
C


ATOM
7182
CD
ARG
A
449
46.598
−12.016
28.311
1.00
48.80
C


ATOM
7185
NE
ARG
A
449
47.788
−12.251
27.482
1.00
53.07
N


ATOM
7187
CZ
ARG
A
449
47.798
−12.442
26.157
1.00
54.07
C


ATOM
7188
NH1
ARG
A
449
46.678
−12.426
25.433
1.00
53.70
N


ATOM
7191
NH2
ARG
A
449
48.958
−12.648
25.539
1.00
55.16
N


ATOM
7194
C
ARG
A
449
42.695
−14.412
30.287
1.00
41.08
C


ATOM
7195
O
ARG
A
449
42.051
−13.372
30.425
1.00
40.93
O


ATOM
7197
N
GLY
A
450
42.114
−15.581
30.027
1.00
41.08
N


ATOM
7198
CA
GLY
A
450
40.664
−15.737
29.988
1.00
41.35
C


ATOM
7201
C
GLY
A
450
40.008
−15.231
28.714
1.00
41.81
C


ATOM
7202
O
GLY
A
450
38.809
−14.957
28.703
1.00
42.43
O


ATOM
7204
N
GLU
A
451
40.783
−15.106
27.639
1.00
42.01
N


ATOM
7205
CA
GLU
A
451
40.243
−14.698
26.341
1.00
42.21
C


ATOM
7207
CB
GLU
A
451
41.323
−14.027
25.485
1.00
42.94
C


ATOM
7210
CG
GLU
A
451
41.686
−12.618
25.944
1.00
46.13
C


ATOM
7213
CD
GLU
A
451
42.918
−12.064
25.247
1.00
51.32
C


ATOM
7214
OE1
GLU
A
451
43.014
−12.174
24.003
1.00
55.77
O


ATOM
7215
OE2
GLU
A
451
43.793
−11.512
25.946
1.00
54.02
O


ATOM
7216
C
GLU
A
451
39.671
−15.914
25.616
1.00
41.36
C


ATOM
7217
O
GLU
A
451
40.216
−17.018
25.721
1.00
41.67
O


ATOM
7219
N
THR
A
452
38.578
−15.704
24.883
1.00
39.62
N


ATOM
7220
CA
THR
A
452
37.870
−16.789
24.199
1.00
38.05
C


ATOM
7222
CB
THR
A
452
36.359
−16.744
24.516
1.00
38.38
C


ATOM
7224
OG1
THR
A
452
35.815
−15.487
24.099
1.00
39.80
O


ATOM
7226
CG2
THR
A
452
36.112
−16.922
26.014
1.00
38.40
C


ATOM
7230
C
THR
A
452
38.050
−16.790
22.672
1.00
36.23
C


ATOM
7231
O
THR
A
452
37.741
−17.790
22.019
1.00
36.26
O


ATOM
7233
N
ALA
A
453
38.550
−15.689
22.108
1.00
33.88
N


ATOM
7234
CA
ALA
A
453
38.696
−15.557
20.651
1.00
32.34
C


ATOM
7236
CB
ALA
A
453
38.826
−14.081
20.260
1.00
32.25
C


ATOM
7240
C
ALA
A
453
39.893
−16.364
20.137
1.00
30.59
C


ATOM
7241
O
ALA
A
453
40.921
−15.803
19.742
1.00
29.94
O


ATOM
7243
N
ASN
A
454
39.734
−17.685
20.143
1.00
28.78
N


ATOM
7244
CA
ASN
A
454
40.806
−18.619
19.808
1.00
28.21
C


ATOM
7246
CB
ASN
A
454
41.827
−18.703
20.961
1.00
28.44
C


ATOM
7249
CG
ASN
A
454
43.004
−19.643
20.656
1.00
30.86
C


ATOM
7250
OD1
ASN
A
454
42.859
−20.870
20.679
1.00
31.68
O


ATOM
7251
ND2
ASN
A
454
44.178
−19.064
20.394
1.00
26.67
N


ATOM
7254
C
ASN
A
454
40.205
−19.994
19.508
1.00
27.11
C


ATOM
7255
O
ASN
A
454
39.375
−20.496
20.272
1.00
26.51
O


ATOM
7257
N
SER
A
455
40.629
−20.595
18.397
1.00
25.67
N


ATOM
7258
CA
SER
A
455
40.076
−21.868
17.934
1.00
24.70
C


ATOM
7260
CB
SER
A
455
40.839
−22.350
16.701
1.00
24.98
C


ATOM
7263
OG
SER
A
455
40.737
−21.396
15.657
1.00
26.20
O


ATOM
7265
C
SER
A
455
40.091
−22.952
19.008
1.00
23.69
C


ATOM
7266
O
SER
A
455
39.084
−23.631
19.220
1.00
22.56
O


ATOM
7268
N
VAL
A
456
41.233
−23.105
19.682
1.00
23.36
N


ATOM
7269
CA
VAL
A
456
41.387
−24.106
20.747
1.00
22.71
C


ATOM
7271
CB
VAL
A
456
42.810
−24.100
21.347
1.00
22.74
C


ATOM
7273
CG1
VAL
A
456
42.946
−25.179
22.420
1.00
17.64
C


ATOM
7277
CG2
VAL
A
456
43.852
−24.291
20.254
1.00
22.47
C


ATOM
7281
C
VAL
A
456
40.397
−23.849
21.873
1.00
22.79
C


ATOM
7282
O
VAL
A
456
39.705
−24.759
22.329
1.00
22.39
O


ATOM
7284
N
SER
A
457
40.336
−22.597
22.311
1.00
24.04
N


ATOM
7285
CA
SER
A
457
39.400
−22.183
23.358
1.00
25.06
C


ATOM
7287
CB
SER
A
457
39.544
−20.684
23.633
1.00
24.32
C


ATOM
7290
OG
SER
A
457
38.420
−20.192
24.330
1.00
27.39
O


ATOM
7292
C
SER
A
457
37.943
−22.531
23.006
1.00
25.81
C


ATOM
7293
O
SER
A
457
37.251
−23.168
23.802
1.00
25.51
O


ATOM
7295
N
CYS
A
458
37.491
−22.126
21.817
1.00
26.61
N


ATOM
7296
CA
CYS
A
458
36.118
−22.425
21.365
1.00
27.38
C


ATOM
7298
CB
CYS
A
458
35.836
−21.830
19.986
1.00
27.10
C


ATOM
7301
SG
CYS
A
458
35.524
−20.076
20.036
1.00
33.51
S


ATOM
7303
C
CYS
A
458
35.837
−23.917
21.330
1.00
26.81
C


ATOM
7304
O
CYS
A
458
34.720
−24.351
21.627
1.00
26.86
O


ATOM
7306
N
TYR
A
459
36.848
−24.695
20.963
1.00
26.30
N


ATOM
7307
CA
TYR
A
459
36.714
−26.140
20.968
1.00
26.58
C


ATOM
7309
CB
TYR
A
459
37.974
−26.811
20.430
1.00
27.07
C


ATOM
7312
CG
TYR
A
459
37.703
−28.140
19.772
1.00
28.23
C


ATOM
7313
CD1
TYR
A
459
37.722
−29.317
20.509
1.00
29.39
C


ATOM
7315
CE1
TYR
A
459
37.473
−30.543
19.907
1.00
33.43
C


ATOM
7317
CZ
TYR
A
459
37.201
−30.598
18.552
1.00
34.05
C


ATOM
7318
OH
TYR
A
459
36.956
−31.810
17.961
1.00
37.25
O


ATOM
7320
CE2
TYR
A
459
37.177
−29.438
17.796
1.00
33.32
C


ATOM
7322
CD2
TYR
A
459
37.424
−28.217
18.410
1.00
30.09
C


ATOM
7324
C
TYR
A
459
36.415
−26.608
22.386
1.00
26.59
C


ATOM
7325
O
TYR
A
459
35.420
−27.299
22.611
1.00
26.74
O


ATOM
7327
N
MET
A
460
37.254
−26.202
23.340
1.00
26.73
N


ATOM
7328
CA
MET
A
460
37.032
−26.537
24.754
1.00
27.29
C


ATOM
7330
CB
MET
A
460
37.959
−25.746
25.678
1.00
27.58
C


ATOM
7333
CG
MET
A
460
39.424
−26.091
25.575
1.00
28.59
C


ATOM
7336
SD
MET
A
460
40.422
−24.891
26.471
1.00
33.42
S


ATOM
7337
CE
MET
A
460
39.811
−25.085
28.148
1.00
22.63
C


ATOM
7341
C
MET
A
460
35.602
−26.249
25.174
1.00
26.99
C


ATOM
7342
O
MET
A
460
34.963
−27.088
25.797
1.00
27.19
O


ATOM
7344
N
ARG
A
461
35.107
−25.063
24.831
1.00
27.64
N


ATOM
7345
CA
ARG
A
461
33.786
−24.622
25.283
1.00
28.90
C


ATOM
7347
CB
ARG
A
461
33.582
−23.138
25.001
1.00
29.19
C


ATOM
7350
CG
ARG
A
461
34.423
−22.213
25.876
1.00
34.67
C


ATOM
7353
CD
ARG
A
461
34.017
−20.760
25.653
1.00
40.90
C


ATOM
7356
NE
ARG
A
461
34.080
−20.420
24.232
1.00
47.05
N


ATOM
7358
CZ
ARG
A
461
33.450
−19.398
23.650
1.00
51.83
C


ATOM
7359
NH1
ARG
A
461
32.691
−18.560
24.353
1.00
52.55
N


ATOM
7362
NH2
ARG
A
461
33.590
−19.209
22.341
1.00
53.43
N


ATOM
7365
C
ARG
A
461
32.648
−25.433
24.658
1.00
28.92
C


ATOM
7366
O
ARG
A
461
31.759
−25.915
25.374
1.00
28.78
O


ATOM
7368
N
THR
A
462
32.684
−25.591
23.335
1.00
28.97
N


ATOM
7369
CA
THR
A
462
31.671
−26.376
22.619
1.00
28.80
C


ATOM
7371
CB
THR
A
462
31.962
−26.447
21.101
1.00
28.77
C


ATOM
7373
OG1
THR
A
462
32.215
−25.132
20.588
1.00
30.56
O


ATOM
7375
CG2
THR
A
462
30.784
−27.059
20.354
1.00
28.56
C


ATOM
7379
C
THR
A
462
31.597
−27.802
23.170
1.00
28.81
C


ATOM
7380
O
THR
A
462
30.523
−28.272
23.552
1.00
28.93
O


ATOM
7382
N
LYS
A
463
32.750
−28.468
23.237
1.00
28.48
N


ATOM
7383
CA
LYS
A
463
32.826
−29.871
23.666
1.00
28.46
C


ATOM
7385
CB
LYS
A
463
33.985
−30.576
22.947
1.00
28.99
C


ATOM
7388
CG
LYS
A
463
33.895
−30.559
21.423
1.00
31.83
C


ATOM
7391
CD
LYS
A
463
32.798
−31.484
20.899
1.00
34.23
C


ATOM
7394
CE
LYS
A
463
32.884
−31.655
19.378
1.00
36.55
C


ATOM
7397
NZ
LYS
A
463
32.245
−32.930
18.922
1.00
36.99
N


ATOM
7401
C
LYS
A
463
32.985
−30.077
25.183
1.00
27.40
C


ATOM
7402
O
LYS
A
463
33.100
−31.214
25.630
1.00
28.09
O


ATOM
7404
N
GLY
A
464
32.999
−28.999
25.966
1.00
25.81
N


ATOM
7405
CA
GLY
A
464
33.180
−29.101
27.417
1.00
24.54
C


ATOM
7408
C
GLY
A
464
34.334
−30.003
27.828
1.00
24.07
C


ATOM
7409
O
GLY
A
464
34.141
−30.962
28.579
1.00
24.88
O


ATOM
7411
N
ILE
A
465
35.532
−29.694
27.333
1.00
23.11
N


ATOM
7412
CA
ILE
A
465
36.733
−30.501
27.582
1.00
22.58
C


ATOM
7414
CB
ILE
A
465
37.069
−31.390
26.359
1.00
22.56
C


ATOM
7416
CG1
ILE
A
465
37.399
−30.522
25.129
1.00
23.34
C


ATOM
7419
CD1
ILE
A
465
37.588
−31.291
23.838
1.00
23.22
C


ATOM
7423
CG2
ILE
A
465
35.912
−32.343
26.065
1.00
19.03
C


ATOM
7427
C
ILE
A
465
37.937
−29.612
27.914
1.00
23.14
C


ATOM
7428
O
ILE
A
465
37.919
−28.402
27.645
1.00
23.01
O


ATOM
7430
N
SER
A
466
38.985
−30.219
28.475
1.00
23.46
N


ATOM
7431
CA
SER
A
466
40.175
−29.478
28.912
1.00
24.13
C


ATOM
7433
CB
SER
A
466
41.078
−30.368
29.767
1.00
23.99
C


ATOM
7436
OG
SER
A
466
41.779
−31.300
28.962
1.00
24.12
O


ATOM
7438
C
SER
A
466
40.991
−28.944
27.737
1.00
25.51
C


ATOM
7439
O
SER
A
466
40.756
−29.323
26.586
1.00
25.67
O


ATOM
7441
N
GLU
A
467
41.954
−28.072
28.040
1.00
26.32
N


ATOM
7442
CA
GLU
A
467
42.893
−27.579
27.038
1.00
26.67
C


ATOM
7444
CB
GLU
A
467
43.846
−26.522
27.615
1.00
26.94
C


ATOM
7447
CG
GLU
A
467
44.869
−26.009
26.580
1.00
28.82
C


ATOM
7450
CD
GLU
A
467
45.890
−25.028
27.134
1.00
31.99
C


ATOM
7451
OE1
GLU
A
467
45.949
−24.820
28.365
1.00
33.18
O


ATOM
7452
OE2
GLU
A
467
46.651
−24.467
26.315
1.00
36.65
O


ATOM
7453
C
GLU
A
467
43.712
−28.722
26.448
1.00
27.51
C


ATOM
7454
O
GLU
A
467
43.909
−28.778
25.228
1.00
27.65
O


ATOM
7456
N
GLU
A
468
44.198
−29.619
27.307
1.00
28.14
N


ATOM
7457
CA
GLU
A
468
45.045
−30.726
26.852
1.00
28.74
C


ATOM
7459
CB
GLU
A
468
45.509
−31.604
28.027
1.00
29.23
C


ATOM
7462
CG
GLU
A
468
46.695
−32.521
27.680
1.00
33.07
C


ATOM
7465
CD
GLU
A
468
47.081
−33.490
28.806
1.00
39.73
C


ATOM
7466
OE1
GLU
A
468
46.600
−33.326
29.953
1.00
41.82
O


ATOM
7467
OE2
GLU
A
468
47.876
−34.422
28.536
1.00
41.60
O


ATOM
7468
C
GLU
A
468
44.320
−31.568
25.794
1.00
28.05
C


ATOM
7469
O
GLU
A
468
44.910
−31.907
24.762
1.00
27.12
O


ATOM
7471
N
LEU
A
469
43.046
−31.883
26.045
1.00
27.29
N


ATOM
7472
CA
LEU
A
469
42.235
−32.635
25.079
1.00
26.86
C


ATOM
7474
CB
LEU
A
469
40.931
−33.153
25.697
1.00
27.30
C


ATOM
7477
CG
LEU
A
469
40.978
−34.562
26.307
1.00
30.60
C


ATOM
7479
CD1
LEU
A
469
40.877
−34.523
27.846
1.00
32.31
C


ATOM
7483
CD2
LEU
A
469
39.867
−35.431
25.716
1.00
32.48
C


ATOM
7487
C
LEU
A
469
41.918
−31.804
23.840
1.00
26.10
C


ATOM
7488
O
LEU
A
469
42.103
−32.273
22.715
1.00
27.20
O


ATOM
7490
N
ALA
A
470
41.445
−30.577
24.038
1.00
23.96
N


ATOM
7491
CA
ALA
A
470
41.167
−29.683
22.908
1.00
22.98
C


ATOM
7493
CB
ALA
A
470
40.690
−28.335
23.404
1.00
22.29
C


ATOM
7497
C
ALA
A
470
42.392
−29.513
21.992
1.00
22.39
C


ATOM
7498
O
ALA
A
470
42.265
−29.503
20.768
1.00
22.37
O


ATOM
7500
N
THR
A
471
43.572
−29.394
22.595
1.00
21.56
N


ATOM
7501
CA
THR
A
471
44.822
−29.205
21.861
1.00
20.64
C


ATOM
7503
CB
THR
A
471
45.979
−28.953
22.846
1.00
20.31
C


ATOM
7505
OG1
THR
A
471
45.692
−27.771
23.607
1.00
22.15
O


ATOM
7507
CG2
THR
A
471
47.312
−28.793
22.128
1.00
15.59
C


ATOM
7511
C
THR
A
471
45.129
−30.417
20.987
1.00
21.70
C


ATOM
7512
O
THR
A
471
45.395
−30.283
19.791
1.00
21.36
O


ATOM
7514
N
GLU
A
472
45.078
−31.597
21.596
1.00
22.71
N


ATOM
7515
CA
GLU
A
472
45.234
−32.868
20.884
1.00
23.00
C


ATOM
7517
CB
GLU
A
472
45.001
−34.021
21.868
1.00
24.07
C


ATOM
7520
CG
GLU
A
472
45.424
−35.402
21.377
1.00
30.42
C


ATOM
7523
CD
GLU
A
472
45.044
−36.498
22.363
1.00
36.53
C


ATOM
7524
OE1
GLU
A
472
45.271
−36.319
23.584
1.00
38.66
O


ATOM
7525
OE2
GLU
A
472
44.511
−37.534
21.913
1.00
41.79
O


ATOM
7526
C
GLU
A
472
44.246
−32.964
19.710
1.00
21.89
C


ATOM
7527
O
GLU
A
472
44.637
−33.288
18.587
1.00
20.82
O


ATOM
7529
N
SER
A
473
42.974
−32.663
19.986
1.00
20.61
N


ATOM
7530
CA
SER
A
473
41.916
−32.661
18.973
1.00
19.87
C


ATOM
7532
CB
SER
A
473
40.581
−32.227
19.582
1.00
19.51
C


ATOM
7535
OG
SER
A
473
40.062
−33.213
20.454
1.00
22.15
O


ATOM
7537
C
SER
A
473
42.235
−31.749
17.797
1.00
19.71
C


ATOM
7538
O
SER
A
473
41.918
−32.071
16.653
1.00
19.59
O


ATOM
7540
N
VAL
A
474
42.847
−30.605
18.080
1.00
19.65
N


ATOM
7541
CA
VAL
A
474
43.264
−29.695
17.021
1.00
19.59
C


ATOM
7543
CB
VAL
A
474
43.553
−28.291
17.559
1.00
19.88
C


ATOM
7545
CG1
VAL
A
474
44.178
−27.432
16.480
1.00
18.67
C


ATOM
7549
CG2
VAL
A
474
42.264
−27.658
18.070
1.00
16.28
C


ATOM
7553
C
VAL
A
474
44.475
−30.237
16.260
1.00
20.08
C


ATOM
7554
O
VAL
A
474
44.597
−30.002
15.061
1.00
20.61
O


ATOM
7556
N
MET
A
475
45.351
−30.977
16.938
1.00
21.01
N


ATOM
7557
CA
MET
A
475
46.462
−31.651
16.252
1.00
21.55
C


ATOM
7559
CB
MET
A
475
47.414
−32.341
17.234
1.00
22.63
C


ATOM
7562
CG
MET
A
475
47.997
−31.482
18.367
1.00
30.07
C


ATOM
7565
SD
MET
A
475
49.500
−30.595
17.922
1.00
47.99
S


ATOM
7566
CE
MET
A
475
50.479
−30.884
19.398
1.00
40.84
C


ATOM
7570
C
MET
A
475
45.906
−32.700
15.277
1.00
21.02
C


ATOM
7571
O
MET
A
475
46.397
−32.843
14.161
1.00
21.64
O


ATOM
7573
N
ASN
A
476
44.881
−33.434
15.697
1.00
20.62
N


ATOM
7574
CA
ASN
A
476
44.282
−34.457
14.839
1.00
20.93
C


ATOM
7576
CB
ASN
A
476
43.254
−35.285
15.610
1.00
20.98
C


ATOM
7579
CG
ASN
A
476
43.895
−36.197
16.630
1.00
22.89
C


ATOM
7580
OD1
ASN
A
476
45.036
−36.633
16.450
1.00
26.51
O


ATOM
7581
ND2
ASN
A
476
43.169
−36.493
17.713
1.00
17.27
N


ATOM
7584
C
ASN
A
476
43.632
−33.844
13.618
1.00
20.64
C


ATOM
7585
O
ASN
A
476
43.672
−34.402
12.526
1.00
18.83
O


ATOM
7587
N
LEU
A
477
43.040
−32.678
13.818
1.00
21.79
N


ATOM
7588
CA
LEU
A
477
42.367
−31.972
12.751
1.00
22.89
C


ATOM
7590
CB
LEU
A
477
41.603
−30.790
13.346
1.00
23.74
C


ATOM
7593
CG
LEU
A
477
40.622
−29.999
12.477
1.00
29.60
C


ATOM
7595
CD1
LEU
A
477
40.042
−30.830
11.317
1.00
34.90
C


ATOM
7599
CD2
LEU
A
477
39.511
−29.441
13.373
1.00
29.89
C


ATOM
7603
C
LEU
A
477
43.382
−31.522
11.703
1.00
22.43
C


ATOM
7604
O
LEU
A
477
43.102
−31.516
10.499
1.00
22.51
O


ATOM
7606
N
ILE
A
478
44.573
−31.164
12.171
1.00
21.44
N


ATOM
7607
CA
ILE
A
478
45.642
−30.754
11.286
1.00
20.64
C


ATOM
7609
CB
ILE
A
478
46.809
−30.120
12.072
1.00
20.87
C


ATOM
7611
CG1
ILE
A
478
46.393
−28.737
12.583
1.00
21.03
C


ATOM
7614
CD1
ILE
A
478
47.353
−28.116
13.594
1.00
19.55
C


ATOM
7618
CG2
ILE
A
478
48.048
−30.001
11.194
1.00
18.72
C


ATOM
7622
C
ILE
A
478
46.130
−31.951
10.483
1.00
20.33
C


ATOM
7623
O
ILE
A
478
46.282
−31.855
9.267
1.00
19.87
O


ATOM
7625
N
ASP
A
479
46.370
−33.074
11.160
1.00
19.79
N


ATOM
7626
CA
ASP
A
479
46.816
−34.291
10.473
1.00
20.12
C


ATOM
7628
CB
ASP
A
479
47.015
−35.459
11.449
1.00
20.05
C


ATOM
7631
CG
ASP
A
479
48.220
−35.277
12.376
1.00
23.05
C


ATOM
7632
OD1
ASP
A
479
49.212
−34.626
11.987
1.00
25.33
O


ATOM
7633
OD2
ASP
A
479
48.181
−35.812
13.511
1.00
30.92
O


ATOM
7634
C
ASP
A
479
45.803
−34.683
9.393
1.00
19.28
C


ATOM
7635
O
ASP
A
479
46.178
−34.989
8.258
1.00
18.51
O


ATOM
7637
N
GLU
A
480
44.524
−34.647
9.759
1.00
19.19
N


ATOM
7638
CA
GLU
A
480
43.424
−34.983
8.861
1.00
19.72
C


ATOM
7640
CB
GLU
A
480
42.106
−34.917
9.632
1.00
21.23
C


ATOM
7643
CG
GLU
A
480
40.879
−35.315
8.839
1.00
29.29
C


ATOM
7646
CD
GLU
A
480
39.640
−35.419
9.711
1.00
37.36
C


ATOM
7647
OE1
GLU
A
480
39.567
−36.368
10.528
1.00
41.70
O


ATOM
7648
OE2
GLU
A
480
38.743
−34.555
9.573
1.00
39.55
O


ATOM
7649
C
GLU
A
480
43.395
−34.059
7.642
1.00
17.87
C


ATOM
7650
O
GLU
A
480
43.318
−34.522
6.502
1.00
16.40
O


ATOM
7652
N
THR
A
481
43.485
−32.758
7.887
1.00
16.85
N


ATOM
7653
CA
THR
A
481
43.599
−31.774
6.806
1.00
16.53
C


ATOM
7655
CB
THR
A
481
43.710
−30.341
7.371
1.00
16.09
C


ATOM
7657
OG1
THR
A
481
42.582
−30.075
8.213
1.00
16.37
O


ATOM
7659
CG2
THR
A
481
43.738
−29.323
6.258
1.00
11.58
C


ATOM
7663
C
THR
A
481
44.779
−32.066
5.855
1.00
16.79
C


ATOM
7664
O
THR
A
481
44.632
−31.976
4.628
1.00
17.41
O


ATOM
7666
N
TRP
A
482
45.936
−32.432
6.404
1.00
15.60
N


ATOM
7667
CA
TRP
A
482
47.075
−32.809
5.560
1.00
15.80
C


ATOM
7669
CB
TRP
A
482
48.326
−33.108
6.397
1.00
16.21
C


ATOM
7672
CG
TRP
A
482
49.175
−31.904
6.612
1.00
15.02
C


ATOM
7673
CD1
TRP
A
482
49.213
−31.115
7.721
1.00
16.82
C


ATOM
7675
NE1
TRP
A
482
50.103
−30.091
7.544
1.00
17.08
N


ATOM
7677
CE2
TRP
A
482
50.659
−30.200
6.301
1.00
17.12
C


ATOM
7678
CD2
TRP
A
482
50.095
−31.337
5.685
1.00
14.82
C


ATOM
7679
CE3
TRP
A
482
50.500
−31.678
4.395
1.00
20.11
C


ATOM
7681
CZ3
TRP
A
482
51.445
−30.889
3.771
1.00
19.38
C


ATOM
7683
CH2
TRP
A
482
51.990
−29.762
4.412
1.00
19.29
C


ATOM
7685
CZ2
TRP
A
482
51.607
−29.403
5.673
1.00
16.00
C


ATOM
7687
C
TRP
A
482
46.754
−33.998
4.647
1.00
16.39
C


ATOM
7688
O
TRP
A
482
47.137
−33.994
3.471
1.00
15.85
O


ATOM
7690
N
LYS
A
483
46.055
−35.003
5.182
1.00
15.77
N


ATOM
7691
CA
LYS
A
483
45.663
−36.171
4.386
1.00
16.30
C


ATOM
7693
CB
LYS
A
483
44.863
−37.180
5.224
1.00
16.44
C


ATOM
7696
CG
LYS
A
483
45.672
−37.910
6.285
1.00
16.04
C


ATOM
7699
CD
LYS
A
483
44.763
−38.613
7.264
1.00
17.03
C


ATOM
7702
CE
LYS
A
483
45.543
−39.387
8.318
1.00
18.84
C


ATOM
7705
NZ
LYS
A
483
44.649
−40.266
9.136
1.00
16.98
N


ATOM
7709
C
LYS
A
483
44.847
−35.743
3.168
1.00
16.93
C


ATOM
7710
O
LYS
A
483
45.089
−36.214
2.062
1.00
16.54
O


ATOM
7712
N
LYS
A
484
43.893
−34.839
3.384
1.00
18.05
N


ATOM
7713
CA
LYS
A
484
43.064
−34.301
2.303
1.00
18.18
C


ATOM
7715
CB
LYS
A
484
41.936
−33.442
2.867
1.00
18.45
C


ATOM
7718
CG
LYS
A
484
40.857
−34.248
3.547
1.00
18.57
C


ATOM
7721
CD
LYS
A
484
39.842
−33.382
4.264
1.00
19.33
C


ATOM
7724
CE
LYS
A
484
38.807
−34.231
4.976
1.00
21.41
C


ATOM
7727
NZ
LYS
A
484
38.185
−33.491
6.094
1.00
26.22
N


ATOM
7731
C
LYS
A
484
43.860
−33.494
1.275
1.00
18.62
C


ATOM
7732
O
LYS
A
484
43.608
−33.606
0.075
1.00
19.13
O


ATOM
7734
N
MET
A
485
44.811
−32.681
1.731
1.00
18.03
N


ATOM
7735
CA
MET
A
485
45.677
−31.950
0.796
1.00
17.61
C


ATOM
7737
CB
MET
A
485
46.591
−30.959
1.517
1.00
16.44
C


ATOM
7740
CG
MET
A
485
45.877
−29.817
2.185
1.00
17.69
C


ATOM
7743
SD
MET
A
485
47.004
−28.497
2.661
1.00
17.20
S


ATOM
7744
CE
MET
A
485
48.111
−29.345
3.795
1.00
13.42
C


ATOM
7748
C
MET
A
485
46.550
−32.913
0.006
1.00
18.05
C


ATOM
7749
O
MET
A
485
46.803
−32.689
−1.170
1.00
19.86
O


ATOM
7751
N
ASN
A
486
47.022
−33.971
0.660
1.00
17.77
N


ATOM
7752
CA
ASN
A
486
47.872
−34.946
0.003
1.00
17.80
C


ATOM
7754
CB
ASN
A
486
48.325
−36.031
0.982
1.00
17.37
C


ATOM
7757
CG
ASN
A
486
49.317
−35.522
2.001
1.00
16.94
C


ATOM
7758
OD1
ASN
A
486
49.989
−34.502
1.789
1.00
15.02
O


ATOM
7759
ND2
ASN
A
486
49.418
−36.230
3.123
1.00
7.80
N


ATOM
7762
C
ASN
A
486
47.150
−35.594
−1.162
1.00
18.79
C


ATOM
7763
O
ASN
A
486
47.756
−35.846
−2.207
1.00
18.53
O


ATOM
7765
N
LYS
A
487
45.859
−35.867
−0.970
1.00
19.13
N


ATOM
7766
CA
LYS
A
487
45.028
−36.466
−2.009
1.00
19.86
C


ATOM
7768
CB
LYS
A
487
43.661
−36.864
−1.442
1.00
20.17
C


ATOM
7771
CG
LYS
A
487
42.715
−37.453
−2.480
1.00
21.08
C


ATOM
7774
CD
LYS
A
487
41.541
−38.168
−1.858
1.00
22.67
C


ATOM
7777
CE
LYS
A
487
40.606
−38.668
−2.937
1.00
25.20
C


ATOM
7780
NZ
LYS
A
487
39.380
−39.301
−2.397
1.00
27.35
N


ATOM
7784
C
LYS
A
487
44.853
−35.508
−3.184
1.00
20.99
C


ATOM
7785
O
LYS
A
487
44.919
−35.917
−4.342
1.00
20.73
O


ATOM
7787
N
GLU
A
488
44.632
−34.231
−2.879
1.00
22.48
N


ATOM
7788
CA
GLU
A
488
44.454
−33.211
−3.906
1.00
23.99
C


ATOM
7790
CB
GLU
A
488
44.145
−31.851
−3.271
1.00
26.10
C


ATOM
7793
CG
GLU
A
488
43.663
−30.775
−4.249
1.00
32.01
C


ATOM
7796
CD
GLU
A
488
42.360
−31.138
−4.954
1.00
37.30
C


ATOM
7797
OE1
GLU
A
488
41.597
−31.989
−4.438
1.00
42.21
O


ATOM
7798
OE2
GLU
A
488
42.104
−30.565
−6.032
1.00
41.25
O


ATOM
7799
C
GLU
A
488
45.699
−33.096
−4.772
1.00
23.29
C


ATOM
7800
O
GLU
A
488
45.603
−33.021
−5.992
1.00
24.93
O


ATOM
7802
N
LYS
A
489
46.868
−33.096
−4.148
1.00
21.28
N


ATOM
7803
CA
LYS
A
489
48.110
−33.036
−4.905
1.00
21.84
C


ATOM
7805
CB
LYS
A
489
49.296
−32.802
−3.956
1.00
21.21
C


ATOM
7808
CG
LYS
A
489
50.660
−32.658
−4.633
1.00
22.30
C


ATOM
7811
CD
LYS
A
489
50.726
−31.466
−5.582
1.00
23.69
C


ATOM
7814
CE
LYS
A
489
51.964
−31.513
−6.476
1.00
26.17
C


ATOM
7817
NZ
LYS
A
489
53.233
−31.562
−5.701
1.00
26.97
N


ATOM
7821
C
LYS
A
489
48.334
−34.295
−5.769
1.00
22.22
C


ATOM
7822
O
LYS
A
489
48.826
−34.199
−6.885
1.00
22.17
O


ATOM
7824
N
LEU
A
490
47.955
−35.461
−5.251
1.00
23.59
N


ATOM
7825
CA
LEU
A
490
48.236
−36.739
−5.903
1.00
25.11
C


ATOM
7827
CB
LEU
A
490
48.206
−37.869
−4.868
1.00
24.75
C


ATOM
7830
CG
LEU
A
490
48.588
−39.255
−5.381
1.00
24.96
C


ATOM
7832
CD1
LEU
A
490
50.037
−39.251
−5.794
1.00
28.66
C


ATOM
7836
CD2
LEU
A
490
48.334
−40.326
−4.334
1.00
26.08
C


ATOM
7840
C
LEU
A
490
47.254
−37.025
−7.049
1.00
27.22
C


ATOM
7841
O
LEU
A
490
47.668
−37.174
−8.199
1.00
26.80
O


ATOM
7843
N
SER
A
491
45.967
−37.117
−6.711
1.00
30.11
N


ATOM
7844
CA
SER
A
491
44.871
−37.239
−7.677
1.00
32.75
C


ATOM
7846
CB
SER
A
491
43.696
−38.004
−7.058
1.00
32.45
C


ATOM
7849
OG
SER
A
491
44.153
−39.120
−6.314
1.00
33.27
O


ATOM
7851
C
SER
A
491
44.482
−35.807
−7.993
1.00
35.81
C


ATOM
7852
O
SER
A
491
45.366
−34.976
−8.190
1.00
39.71
O


ATOM
7854
N
GLY
A
492
43.194
−35.488
−8.031
1.00
36.89
N


ATOM
7855
CA
GLY
A
492
42.782
−34.083
−7.975
1.00
38.51
C


ATOM
7858
C
GLY
A
492
42.948
−33.426
−9.324
1.00
39.51
C


ATOM
7859
O
GLY
A
492
44.023
−33.483
−9.926
1.00
39.21
O


ATOM
7861
N
SER
A
493
41.885
−32.785
−9.793
1.00
40.76
N


ATOM
7862
CA
SER
A
493
41.744
−32.527
−11.221
1.00
42.11
C


ATOM
7864
CB
SER
A
493
40.536
−33.308
−11.779
1.00
42.40
C


ATOM
7867
OG
SER
A
493
39.367
−33.119
−10.991
1.00
42.24
O


ATOM
7869
C
SER
A
493
41.661
−31.055
−11.620
1.00
42.90
C


ATOM
7870
O
SER
A
493
42.195
−30.688
−12.667
1.00
45.63
O


ATOM
7872
N
LEU
A
494
41.019
−30.214
−10.812
1.00
41.80
N


ATOM
7873
CA
LEU
A
494
40.745
−28.833
−11.234
1.00
41.69
C


ATOM
7875
CB
LEU
A
494
39.819
−28.134
−10.234
1.00
42.85
C


ATOM
7878
CG
LEU
A
494
38.410
−28.738
−10.171
1.00
48.25
C


ATOM
7880
CD1
LEU
A
494
37.681
−28.348
−8.874
1.00
51.97
C


ATOM
7884
CD2
LEU
A
494
37.591
−28.341
−11.407
1.00
50.78
C


ATOM
7888
C
LEU
A
494
42.019
−28.002
−11.465
1.00
39.71
C


ATOM
7889
O
LEU
A
494
42.341
−27.665
−12.613
1.00
39.89
O


ATOM
7891
N
PHE
A
495
42.741
−27.697
−10.385
1.00
36.46
N


ATOM
7892
CA
PHE
A
495
43.918
−26.830
−10.449
1.00
33.36
C


ATOM
7894
CB
PHE
A
495
44.174
−26.182
−9.091
1.00
33.16
C


ATOM
7897
CG
PHE
A
495
43.100
−25.232
−8.675
1.00
30.77
C


ATOM
7898
CD1
PHE
A
495
43.098
−23.929
−9.139
1.00
27.11
C


ATOM
7900
CE1
PHE
A
495
42.110
−23.050
−8.762
1.00
29.33
C


ATOM
7902
CZ
PHE
A
495
41.101
−23.470
−7.922
1.00
30.73
C


ATOM
7904
CE2
PHE
A
495
41.091
−24.769
−7.460
1.00
32.49
C


ATOM
7906
CD2
PHE
A
495
42.085
−25.643
−7.839
1.00
30.06
C


ATOM
7908
C
PHE
A
495
45.173
−27.557
−10.895
1.00
31.67
C


ATOM
7909
O
PHE
A
495
45.347
−28.740
−10.630
1.00
31.25
O


ATOM
7911
N
ALA
A
496
46.057
−26.831
−11.565
1.00
30.99
N


ATOM
7912
CA
ALA
A
496
47.342
−27.383
−11.959
1.00
30.97
C


ATOM
7914
CB
ALA
A
496
48.020
−26.492
−13.009
1.00
30.95
C


ATOM
7918
C
ALA
A
496
48.240
−27.566
−10.733
1.00
30.01
C


ATOM
7919
O
ALA
A
496
48.142
−26.828
−9.750
1.00
28.13
O


ATOM
7921
N
LYS
A
497
49.124
−28.556
−10.823
1.00
29.98
N


ATOM
7922
CA
LYS
A
497
49.983
−28.956
−9.722
1.00
29.48
C


ATOM
7924
CB
LYS
A
497
50.875
−30.129
−10.153
1.00
29.84
C


ATOM
7927
CG
LYS
A
497
50.112
−31.456
−10.266
1.00
36.81
C


ATOM
7930
CD
LYS
A
497
51.052
−32.675
−10.271
1.00
43.99
C


ATOM
7933
CE
LYS
A
497
50.275
−33.997
−10.386
1.00
48.85
C


ATOM
7936
NZ
LYS
A
497
50.937
−35.142
−9.664
1.00
52.33
N


ATOM
7940
C
LYS
A
497
50.817
−27.817
−9.125
1.00
27.48
C


ATOM
7941
O
LYS
A
497
50.933
−27.723
−7.909
1.00
26.08
O


ATOM
7943
N
PRO
A
498
51.397
−26.947
−9.973
1.00
26.23
N


ATOM
7944
CA
PRO
A
498
52.157
−25.819
−9.443
1.00
24.44
C


ATOM
7946
CB
PRO
A
498
52.439
−24.980
−10.689
1.00
24.60
C


ATOM
7949
CG
PRO
A
498
52.485
−25.960
−11.776
1.00
23.99
C


ATOM
7952
CD
PRO
A
498
51.438
−26.970
−11.446
1.00
25.63
C


ATOM
7955
C
PRO
A
498
51.369
−25.004
−8.442
1.00
23.21
C


ATOM
7956
O
PRO
A
498
51.925
−24.597
−7.427
1.00
23.94
O


ATOM
7957
N
PHE
A
499
50.087
−24.771
−8.722
1.00
21.85
N


ATOM
7958
CA
PHE
A
499
49.257
−23.989
−7.818
1.00
21.24
C


ATOM
7960
CB
PHE
A
499
48.010
−23.418
−8.497
1.00
20.57
C


ATOM
7963
CG
PHE
A
499
47.144
−22.647
−7.551
1.00
19.75
C


ATOM
7964
CD1
PHE
A
499
47.593
−21.452
−7.008
1.00
18.26
C


ATOM
7966
CE1
PHE
A
499
46.814
−20.743
−6.090
1.00
18.02
C


ATOM
7968
CZ
PHE
A
499
45.575
−21.237
−5.705
1.00
15.99
C


ATOM
7970
CE2
PHE
A
499
45.131
−22.442
−6.225
1.00
18.43
C


ATOM
7972
CD2
PHE
A
499
45.921
−23.148
−7.134
1.00
19.13
C


ATOM
7974
C
PHE
A
499
48.848
−24.777
−6.576
1.00
21.26
C


ATOM
7975
O
PHE
A
499
48.859
−24.240
−5.479
1.00
21.95
O


ATOM
7977
N
VAL
A
500
48.481
−26.042
−6.739
1.00
20.90
N


ATOM
7978
CA
VAL
A
500
48.164
−26.870
−5.581
1.00
20.48
C


ATOM
7980
CB
VAL
A
500
47.767
−28.309
−5.986
1.00
21.13
C


ATOM
7982
CG1
VAL
A
500
47.639
−29.211
−4.754
1.00
15.52
C


ATOM
7986
CG2
VAL
A
500
46.460
−28.287
−6.774
1.00
21.57
C


ATOM
7990
C
VAL
A
500
49.351
−26.899
−4.624
1.00
19.82
C


ATOM
7991
O
VAL
A
500
49.191
−26.760
−3.414
1.00
19.55
O


ATOM
7993
N
GLU
A
501
50.541
−27.057
−5.183
1.00
19.75
N


ATOM
7994
CA
GLU
A
501
51.755
−27.114
−4.390
1.00
20.23
C


ATOM
7996
CB
GLU
A
501
52.941
−27.494
−5.275
1.00
20.80
C


ATOM
7999
CG
GLU
A
501
54.264
−27.676
−4.545
1.00
23.16
C


ATOM
8002
CD
GLU
A
501
54.295
−28.896
−3.648
1.00
27.14
C


ATOM
8003
OE1
GLU
A
501
53.352
−29.734
−3.686
1.00
27.24
O


ATOM
8004
OE2
GLU
A
501
55.282
−29.003
−2.896
1.00
28.49
O


ATOM
8005
C
GLU
A
501
52.016
−25.781
−3.698
1.00
19.44
C


ATOM
8006
O
GLU
A
501
52.481
−25.756
−2.562
1.00
19.54
O


ATOM
8008
N
THR
A
502
51.725
−24.680
−4.382
1.00
18.86
N


ATOM
8009
CA
THR
A
502
51.856
−23.362
−3.772
1.00
18.38
C


ATOM
8011
CB
THR
A
502
51.539
−22.251
−4.763
1.00
18.24
C


ATOM
8013
OG1
THR
A
502
52.503
−22.282
−5.823
1.00
20.38
O


ATOM
8015
CG2
THR
A
502
51.588
−20.902
−4.081
1.00
19.60
C


ATOM
8019
C
THR
A
502
50.920
−23.259
−2.579
1.00
18.14
C


ATOM
8020
O
THR
A
502
51.323
−22.821
−1.501
1.00
18.35
O


ATOM
8022
N
ALA
A
503
49.683
−23.704
−2.772
1.00
17.11
N


ATOM
8023
CA
ALA
A
503
48.687
−23.684
−1.711
1.00
17.25
C


ATOM
8025
CB
ALA
A
503
47.343
−24.182
−2.228
1.00
16.45
C


ATOM
8029
C
ALA
A
503
49.165
−24.508
−0.511
1.00
17.44
C


ATOM
8030
O
ALA
A
503
49.188
−24.011
0.607
1.00
18.65
O


ATOM
8032
N
ILE
A
504
49.585
−25.747
−0.749
1.00
17.37
N


ATOM
8033
CA
ILE
A
504
50.147
−26.579
0.320
1.00
17.19
C


ATOM
8035
CB
ILE
A
504
50.598
−27.970
−0.205
1.00
17.91
C


ATOM
8037
CG1
ILE
A
504
49.376
−28.836
−0.562
1.00
20.02
C


ATOM
8040
CD1
ILE
A
504
49.670
−30.002
−1.524
1.00
13.50
C


ATOM
8044
CG2
ILE
A
504
51.448
−28.702
0.838
1.00
17.57
C


ATOM
8048
C
ILE
A
504
51.309
−25.872
1.044
1.00
16.63
C


ATOM
8049
O
ILE
A
504
51.448
−25.996
2.275
1.00
13.35
O


ATOM
8051
N
ASN
A
505
52.115
−25.111
0.294
1.00
16.69
N


ATOM
8052
CA
ASN
A
505
53.260
−24.405
0.885
1.00
17.13
C


ATOM
8054
CB
ASN
A
505
54.120
−23.736
−0.182
1.00
17.81
C


ATOM
8057
CG
ASN
A
505
54.947
−24.727
−0.980
1.00
18.31
C


ATOM
8058
OD1
ASN
A
505
55.281
−25.811
−0.501
1.00
22.01
O


ATOM
8059
ND2
ASN
A
505
55.280
−24.357
−2.205
1.00
15.11
N


ATOM
8062
C
ASN
A
505
52.873
−23.378
1.941
1.00
17.83
C


ATOM
8063
O
ASN
A
505
53.645
−23.122
2.859
1.00
18.43
O


ATOM
8065
N
LEU
A
506
51.684
−22.796
1.834
1.00
18.40
N


ATOM
8066
CA
LEU
A
506
51.235
−21.861
2.858
1.00
18.74
C


ATOM
8068
CB
LEU
A
506
49.973
−21.106
2.416
1.00
20.63
C


ATOM
8071
CG
LEU
A
506
49.365
−20.100
3.419
1.00
20.63
C


ATOM
8073
CD1
LEU
A
506
49.177
−18.729
2.800
1.00
17.23
C


ATOM
8077
CD2
LEU
A
506
48.044
−20.623
3.945
1.00
17.29
C


ATOM
8081
C
LEU
A
506
51.022
−22.598
4.178
1.00
17.22
C


ATOM
8082
O
LEU
A
506
51.364
−22.083
5.235
1.00
16.26
O


ATOM
8084
N
ALA
A
507
50.492
−23.815
4.111
1.00
16.66
N


ATOM
8085
CA
ALA
A
507
50.397
−24.666
5.300
1.00
15.83
C


ATOM
8087
CB
ALA
A
507
49.675
−25.972
4.984
1.00
14.75
C


ATOM
8091
C
ALA
A
507
51.780
−24.946
5.862
1.00
16.10
C


ATOM
8092
O
ALA
A
507
51.986
−24.878
7.071
1.00
16.30
O


ATOM
8094
N
ARG
A
508
52.731
−25.251
4.979
1.00
17.61
N


ATOM
8095
CA
ARG
A
508
54.116
−25.504
5.392
1.00
17.53
C


ATOM
8097
CB
ARG
A
508
54.987
−25.888
4.205
1.00
17.44
C


ATOM
8100
CG
ARG
A
508
54.619
−27.205
3.595
1.00
21.11
C


ATOM
8103
CD
ARG
A
508
55.602
−27.634
2.531
1.00
19.20
C


ATOM
8106
NE
ARG
A
508
55.335
−29.024
2.160
1.00
21.78
N


ATOM
8108
CZ
ARG
A
508
54.926
−29.456
0.968
1.00
19.87
C


ATOM
8109
NH1
ARG
A
508
54.742
−28.624
−0.051
1.00
17.50
N


ATOM
8112
NH2
ARG
A
508
54.718
−30.755
0.792
1.00
21.11
N


ATOM
8115
C
ARG
A
508
54.700
−24.280
6.054
1.00
18.16
C


ATOM
8116
O
ARG
A
508
55.252
−24.367
7.148
1.00
18.30
O


ATOM
8118
N
GLN
A
509
54.565
−23.134
5.396
1.00
18.99
N


ATOM
8119
CA
GLN
A
509
55.054
−21.879
5.960
1.00
20.33
C


ATOM
8121
CB
GLN
A
509
54.765
−20.717
5.011
1.00
20.49
C


ATOM
8124
CG
GLN
A
509
55.204
−19.360
5.523
1.00
22.03
C


ATOM
8127
CD
GLN
A
509
56.678
−19.288
5.821
1.00
21.97
C


ATOM
8128
OE1
GLN
A
509
57.506
−19.664
4.999
1.00
27.73
O


ATOM
8129
NE2
GLN
A
509
57.019
−18.789
6.998
1.00
29.26
N


ATOM
8132
C
GLN
A
509
54.450
−21.604
7.345
1.00
21.32
C


ATOM
8133
O
GLN
A
509
55.134
−21.067
8.219
1.00
22.25
O


ATOM
8135
N
SER
A
510
53.184
−21.980
7.541
1.00
20.79
N


ATOM
8136
CA
SER
A
510
52.524
−21.826
8.832
1.00
20.10
C


ATOM
8138
CB
SER
A
510
51.047
−22.189
8.720
1.00
19.90
C


ATOM
8141
OG
SER
A
510
50.416
−21.459
7.692
1.00
18.71
O


ATOM
8143
C
SER
A
510
53.183
−22.696
9.904
1.00
20.38
C


ATOM
8144
O
SER
A
510
53.431
−22.243
11.020
1.00
19.76
O


ATOM
8146
N
HIS
A
511
53.471
−23.945
9.561
1.00
20.90
N


ATOM
8147
CA
HIS
A
511
54.139
−24.851
10.499
1.00
22.22
C


ATOM
8149
CB
HIS
A
511
54.324
−26.250
9.894
1.00
22.59
C


ATOM
8152
CG
HIS
A
511
53.096
−27.095
9.951
1.00
21.60
C


ATOM
8153
ND1
HIS
A
511
52.612
−27.618
11.131
1.00
21.90
N


ATOM
8155
CE1
HIS
A
511
51.521
−28.319
10.883
1.00
22.43
C


ATOM
8157
NE2
HIS
A
511
51.278
−28.266
9.586
1.00
24.05
N


ATOM
8159
CD2
HIS
A
511
52.248
−27.504
8.979
1.00
22.11
C


ATOM
8161
C
HIS
A
511
55.497
−24.328
10.939
1.00
22.27
C


ATOM
8162
O
HIS
A
511
55.908
−24.552
12.068
1.00
22.96
O


ATOM
8164
N
CYS
A
512
56.191
−23.645
10.038
1.00
23.22
N


ATOM
8165
CA
CYS
A
512
57.523
−23.129
10.321
1.00
24.23
C


ATOM
8167
CB
CYS
A
512
58.329
−23.010
9.031
1.00
24.32
C


ATOM
8170
SG
CYS
A
512
58.656
−24.595
8.249
1.00
22.30
S


ATOM
8172
C
CYS
A
512
57.479
−21.782
11.024
1.00
25.86
C


ATOM
8173
O
CYS
A
512
58.350
−21.495
11.837
1.00
26.42
O


ATOM
8175
N
THR
A
513
56.482
−20.957
10.705
1.00
27.61
N


ATOM
8176
CA
THR
A
513
56.318
−19.654
11.361
1.00
29.15
C


ATOM
8178
CB
THR
A
513
55.331
−18.741
10.593
1.00
29.25
C


ATOM
8180
OG1
THR
A
513
55.971
−18.221
9.422
1.00
28.09
O


ATOM
8182
CG2
THR
A
513
54.873
−17.577
11.464
1.00
26.32
C


ATOM
8186
C
THR
A
513
55.870
−19.769
12.830
1.00
31.47
C


ATOM
8187
O
THR
A
513
56.363
−19.028
13.683
1.00
30.56
O


ATOM
8189
N
TYR
A
514
54.955
−20.694
13.126
1.00
34.30
N


ATOM
8190
CA
TYR
A
514
54.379
−20.792
14.479
1.00
35.87
C


ATOM
8192
CB
TYR
A
514
52.847
−20.940
14.416
1.00
34.25
C


ATOM
8195
CG
TYR
A
514
52.221
−19.733
13.751
1.00
30.20
C


ATOM
8196
CD1
TYR
A
514
52.264
−18.478
14.363
1.00
31.58
C


ATOM
8198
CE1
TYR
A
514
51.724
−17.349
13.747
1.00
29.95
C


ATOM
8200
CZ
TYR
A
514
51.142
−17.468
12.506
1.00
32.59
C


ATOM
8201
OH
TYR
A
514
50.615
−16.358
11.893
1.00
34.88
O


ATOM
8203
CE2
TYR
A
514
51.094
−18.703
11.872
1.00
32.82
C


ATOM
8205
CD2
TYR
A
514
51.641
−19.822
12.494
1.00
30.70
C


ATOM
8207
C
TYR
A
514
55.075
−21.864
15.318
1.00
39.29
C


ATOM
8208
O
TYR
A
514
54.486
−22.865
15.728
1.00
39.16
O


ATOM
8210
N
HIS
A
515
56.349
−21.596
15.587
1.00
43.59
N


ATOM
8211
CA
HIS
A
515
57.228
−22.479
16.359
1.00
46.79
C


ATOM
8213
CB
HIS
A
515
58.604
−22.525
15.688
1.00
46.96
C


ATOM
8216
CG
HIS
A
515
59.284
−21.188
15.629
1.00
50.00
C


ATOM
8217
ND1
HIS
A
515
59.273
−20.400
14.498
1.00
50.33
N


ATOM
8219
CE1
HIS
A
515
59.930
−19.279
14.738
1.00
52.29
C


ATOM
8221
NE2
HIS
A
515
60.357
−19.306
15.989
1.00
52.99
N


ATOM
8223
CD2
HIS
A
515
59.961
−20.486
16.570
1.00
52.28
C


ATOM
8225
C
HIS
A
515
57.408
−21.967
17.791
1.00
48.92
C


ATOM
8226
O
HIS
A
515
56.807
−20.962
18.187
1.00
48.55
O


ATOM
8228
N
ASN
A
516
58.242
−22.680
18.551
1.00
51.56
N


ATOM
8229
CA
ASN
A
516
58.764
−22.219
19.842
1.00
53.22
C


ATOM
8231
CB
ASN
A
516
57.896
−22.727
21.001
1.00
53.73
C


ATOM
8234
CG
ASN
A
516
56.965
−21.661
21.543
1.00
55.64
C


ATOM
8235
OD1
ASN
A
516
57.063
−21.274
22.708
1.00
57.77
O


ATOM
8236
ND2
ASN
A
516
56.064
−21.173
20.698
1.00
56.49
N


ATOM
8239
C
ASN
A
516
60.214
−22.679
20.035
1.00
54.14
C


ATOM
8240
O
ASN
A
516
61.060
−21.929
20.529
1.00
54.93
O


ATOM
8242
N
THR
A
521
67.064
−21.371
22.423
1.00
68.24
N


ATOM
8243
CA
THR
A
521
66.660
−20.234
21.600
1.00
68.31
C


ATOM
8245
CB
THR
A
521
66.789
−20.549
20.083
1.00
68.39
C


ATOM
8247
OG1
THR
A
521
67.897
−21.429
19.852
1.00
68.01
O


ATOM
8249
CG2
THR
A
521
66.985
−19.266
19.278
1.00
68.04
C


ATOM
8253
C
THR
A
521
65.209
−19.850
21.908
1.00
68.29
C


ATOM
8254
O
THR
A
521
64.321
−20.708
21.905
1.00
68.26
O


ATOM
8256
N
SER
A
522
64.976
−18.563
22.166
1.00
68.25
N


ATOM
8257
CA
SER
A
522
63.631
−18.058
22.473
1.00
68.36
C


ATOM
8259
CB
SER
A
522
63.714
−16.694
23.171
1.00
68.48
C


ATOM
8262
OG
SER
A
522
63.836
−15.635
22.234
1.00
67.70
O


ATOM
8264
C
SER
A
522
62.809
−17.947
21.186
1.00
68.90
C


ATOM
8265
O
SER
A
522
63.381
−17.966
20.091
1.00
68.97
O


ATOM
8267
N
PRO
A
523
61.468
−17.822
21.308
1.00
69.43
N


ATOM
8268
CA
PRO
A
523
60.602
−17.766
20.117
1.00
69.50
C


ATOM
8270
CB
PRO
A
523
59.226
−17.386
20.690
1.00
69.57
C


ATOM
8273
CG
PRO
A
523
59.253
−17.849
22.099
1.00
69.97
C


ATOM
8276
CD
PRO
A
523
60.687
−17.777
22.561
1.00
69.52
C


ATOM
8279
C
PRO
A
523
61.065
−16.739
19.075
1.00
69.50
C


ATOM
8280
O
PRO
A
523
61.295
−17.101
17.915
1.00
69.97
O


ATOM
8281
N
ASP
A
524
61.211
−15.482
19.496
1.00
68.93
N


ATOM
8282
CA
ASP
A
524
61.683
−14.408
18.618
1.00
68.26
C


ATOM
8284
CB
ASP
A
524
60.918
−13.105
18.886
1.00
68.53
C


ATOM
8287
CG
ASP
A
524
59.544
−13.090
18.232
1.00
69.51
C


ATOM
8288
OD1
ASP
A
524
59.451
−13.409
17.022
1.00
68.29
O


ATOM
8289
OD2
ASP
A
524
58.560
−12.753
18.927
1.00
69.69
O


ATOM
8290
C
ASP
A
524
63.185
−14.211
18.809
1.00
67.06
C


ATOM
8291
O
ASP
A
524
63.625
−13.672
19.824
1.00
67.48
O


ATOM
8293
N
GLU
A
525
63.938
−14.637
17.796
1.00
65.37
N


ATOM
8294
CA
GLU
A
525
65.403
−14.833
17.815
1.00
64.34
C


ATOM
8296
CB
GLU
A
525
65.996
−15.095
19.216
1.00
64.46
C


ATOM
8299
CG
GLU
A
525
66.657
−13.867
19.874
1.00
65.56
C


ATOM
8302
CD
GLU
A
525
67.334
−14.182
21.215
1.00
68.38
C


ATOM
8303
OE1
GLU
A
525
67.271
−15.343
21.679
1.00
69.09
O


ATOM
8304
OE2
GLU
A
525
67.934
−13.261
21.812
1.00
69.29
O


ATOM
8305
C
GLU
A
525
65.684
−16.023
16.893
1.00
62.76
C


ATOM
8306
O
GLU
A
525
66.605
−15.985
16.075
1.00
62.36
O


ATOM
8308
N
LEU
A
526
64.879
−17.076
17.041
1.00
60.82
N


ATOM
8309
CA
LEU
A
526
64.851
−18.175
16.079
1.00
59.35
C


ATOM
8311
CB
LEU
A
526
64.027
−19.355
16.621
1.00
59.35
C


ATOM
8314
CG
LEU
A
526
63.869
−20.605
15.735
1.00
59.00
C


ATOM
8316
CD1
LEU
A
526
65.212
−21.129
15.233
1.00
58.18
C


ATOM
8320
CD2
LEU
A
526
63.114
−21.708
16.479
1.00
57.29
C


ATOM
8324
C
LEU
A
526
64.279
−17.675
14.746
1.00
58.24
C


ATOM
8325
O
LEU
A
526
64.798
−18.018
13.680
1.00
58.01
O


ATOM
8327
N
THR
A
527
63.219
−16.864
14.814
1.00
56.56
N


ATOM
8328
CA
THR
A
527
62.641
−16.227
13.626
1.00
55.44
C


ATOM
8330
CB
THR
A
527
61.398
−15.364
13.982
1.00
55.35
C


ATOM
8332
OG1
THR
A
527
60.408
−16.178
14.625
1.00
55.68
O


ATOM
8334
CG2
THR
A
527
60.788
−14.728
12.733
1.00
51.94
C


ATOM
8338
C
THR
A
527
63.679
−15.339
12.941
1.00
55.46
C


ATOM
8339
O
THR
A
527
63.885
−15.431
11.730
1.00
55.61
O


ATOM
8341
N
ARG
A
528
64.337
−14.494
13.731
1.00
55.44
N


ATOM
8342
CA
ARG
A
528
65.379
−13.591
13.232
1.00
55.76
C


ATOM
8344
CB
ARG
A
528
65.896
−12.707
14.379
1.00
56.27
C


ATOM
8347
CG
ARG
A
528
66.933
−11.656
13.983
1.00
59.93
C


ATOM
8350
CD
ARG
A
528
67.442
−10.872
15.201
1.00
65.08
C


ATOM
8353
NE
ARG
A
528
68.019
−11.741
16.236
1.00
69.64
N


ATOM
8355
CZ
ARG
A
528
69.252
−12.259
16.216
1.00
72.42
C


ATOM
8356
NH1
ARG
A
528
70.092
−12.017
15.209
1.00
72.24
N


ATOM
8359
NH2
ARG
A
528
69.653
−13.037
17.219
1.00
72.90
N


ATOM
8362
C
ARG
A
528
66.541
−14.353
12.571
1.00
54.88
C


ATOM
8363
O
ARG
A
528
67.081
−13.896
11.563
1.00
55.27
O


ATOM
8365
N
LYS
A
529
66.918
−15.501
13.139
1.00
53.44
N


ATOM
8366
CA
LYS
A
529
67.976
−16.348
12.565
1.00
52.75
C


ATOM
8368
CB
LYS
A
529
68.468
−17.393
13.580
1.00
52.56
C


ATOM
8371
CG
LYS
A
529
69.462
−16.838
14.594
1.00
54.23
C


ATOM
8374
CD
LYS
A
529
69.995
−17.901
15.556
1.00
54.76
C


ATOM
8377
CE
LYS
A
529
70.895
−17.263
16.621
1.00
55.90
C


ATOM
8380
NZ
LYS
A
529
71.540
−18.258
17.524
1.00
55.71
N


ATOM
8384
C
LYS
A
529
67.528
−17.041
11.274
1.00
52.02
C


ATOM
8385
O
LYS
A
529
68.297
−17.134
10.314
1.00
51.94
O


ATOM
8387
N
ARG
A
530
66.292
−17.535
11.261
1.00
51.11
N


ATOM
8388
CA
ARG
A
530
65.730
−18.162
10.068
1.00
50.43
C


ATOM
8390
CB
ARG
A
530
64.382
−18.827
10.384
1.00
50.26
C


ATOM
8393
CG
ARG
A
530
64.511
−20.115
11.201
1.00
49.87
C


ATOM
8396
CD
ARG
A
530
63.156
−20.742
11.540
1.00
49.26
C


ATOM
8399
NE
ARG
A
530
63.299
−22.047
12.196
1.00
47.40
N


ATOM
8401
CZ
ARG
A
530
62.288
−22.856
12.520
1.00
49.75
C


ATOM
8402
NH1
ARG
A
530
61.028
−22.519
12.251
1.00
50.99
N


ATOM
8405
NH2
ARG
A
530
62.533
−24.020
13.113
1.00
51.56
N


ATOM
8408
C
ARG
A
530
65.591
−17.135
8.937
1.00
50.22
C


ATOM
8409
O
ARG
A
530
65.951
−17.414
7.789
1.00
50.45
O


ATOM
8411
N
VAL
A
531
65.095
−15.944
9.271
1.00
49.41
N


ATOM
8412
CA
VAL
A
531
64.958
−14.857
8.291
1.00
48.42
C


ATOM
8414
CB
VAL
A
531
64.188
−13.636
8.891
1.00
48.66
C


ATOM
8416
CG1
VAL
A
531
64.109
−12.479
7.890
1.00
47.11
C


ATOM
8420
CG2
VAL
A
531
62.788
−14.051
9.329
1.00
47.42
C


ATOM
8424
C
VAL
A
531
66.333
−14.423
7.751
1.00
47.05
C


ATOM
8425
O
VAL
A
531
66.501
−14.245
6.546
1.00
47.38
O


ATOM
8427
N
LEU
A
532
67.309
−14.265
8.641
1.00
45.54
N


ATOM
8428
CA
LEU
A
532
68.670
−13.905
8.232
1.00
44.51
C


ATOM
8430
CB
LEU
A
532
69.604
−13.792
9.447
1.00
44.81
C


ATOM
8433
CG
LEU
A
532
69.906
−12.382
9.972
1.00
46.31
C


ATOM
8435
CD1
LEU
A
532
68.632
−11.547
10.163
1.00
45.86
C


ATOM
8439
CD2
LEU
A
532
70.715
−12.472
11.269
1.00
45.01
C


ATOM
8443
C
LEU
A
532
69.251
−14.909
7.239
1.00
43.07
C


ATOM
8444
O
LEU
A
532
69.769
−14.526
6.189
1.00
42.09
O


ATOM
8446
N
SER
A
533
69.149
−16.193
7.581
1.00
41.93
N


ATOM
8447
CA
SER
A
533
69.714
−17.273
6.765
1.00
40.78
C


ATOM
8449
CB
SER
A
533
69.710
−18.585
7.552
1.00
40.65
C


ATOM
8452
OG
SER
A
533
68.403
−19.135
7.595
1.00
41.90
O


ATOM
8454
C
SER
A
533
68.970
−17.484
5.439
1.00
38.88
C


ATOM
8455
O
SER
A
533
69.523
−18.069
4.508
1.00
38.00
O


ATOM
8457
N
VAL
A
534
67.725
−17.017
5.370
1.00
37.22
N


ATOM
8458
CA
VAL
A
534
66.895
−17.169
4.180
1.00
36.56
C


ATOM
8460
CB
VAL
A
534
65.414
−17.407
4.560
1.00
35.93
C


ATOM
8462
CG1
VAL
A
534
64.484
−17.102
3.392
1.00
32.65
C


ATOM
8466
CG2
VAL
A
534
65.228
−18.829
5.019
1.00
32.66
C


ATOM
8470
C
VAL
A
534
67.013
−15.976
3.231
1.00
37.95
C


ATOM
8471
O
VAL
A
534
67.189
−16.163
2.022
1.00
38.77
O


ATOM
8473
N
ILE
A
535
66.929
−14.761
3.770
1.00
38.59
N


ATOM
8474
CA
ILE
A
535
66.937
−13.554
2.940
1.00
39.64
C


ATOM
8476
CB
ILE
A
535
65.989
−12.474
3.493
1.00
39.52
C


ATOM
8478
CG1
ILE
A
535
64.563
−12.999
3.629
1.00
38.45
C


ATOM
8481
CD1
ILE
A
535
63.899
−13.290
2.315
1.00
41.93
C


ATOM
8485
CG2
ILE
A
535
66.018
−11.239
2.591
1.00
41.43
C


ATOM
8489
C
ILE
A
535
68.318
−12.905
2.814
1.00
40.82
C


ATOM
8490
O
ILE
A
535
68.828
−12.726
1.706
1.00
41.22
O


ATOM
8492
N
THR
A
536
68.912
−12.556
3.953
1.00
41.78
N


ATOM
8493
CA
THR
A
536
69.995
−11.568
3.989
1.00
42.39
C


ATOM
8495
CB
THR
A
536
69.833
−10.632
5.217
1.00
42.80
C


ATOM
8497
OG1
THR
A
536
69.789
−11.410
6.418
1.00
46.53
O


ATOM
8499
CG2
THR
A
536
68.540
−9.814
5.110
1.00
41.77
C


ATOM
8503
C
THR
A
536
71.413
−12.162
3.955
1.00
41.92
C


ATOM
8504
O
THR
A
536
72.239
−11.739
3.143
1.00
42.54
O


ATOM
8506
N
GLU
A
537
71.693
−13.128
4.826
1.00
40.62
N


ATOM
8507
CA
GLU
A
537
73.035
−13.709
4.933
1.00
39.78
C


ATOM
8509
CB
GLU
A
537
73.319
−14.147
6.376
1.00
40.55
C


ATOM
8512
CG
GLU
A
537
73.642
−13.013
7.338
1.00
41.76
C


ATOM
8515
CD
GLU
A
537
73.948
−13.519
8.742
1.00
45.87
C


ATOM
8516
OE1
GLU
A
537
75.134
−13.513
9.139
1.00
48.56
O


ATOM
8517
OE2
GLU
A
537
73.005
−13.939
9.446
1.00
46.99
O


ATOM
8518
C
GLU
A
537
73.209
−14.911
3.992
1.00
38.20
C


ATOM
8519
O
GLU
A
537
72.405
−15.847
4.040
1.00
37.11
O


ATOM
8521
N
PRO
A
538
74.252
−14.889
3.133
1.00
36.56
N


ATOM
8522
CA
PRO
A
538
74.595
−16.059
2.320
1.00
35.27
C


ATOM
8524
CB
PRO
A
538
75.641
−15.518
1.337
1.00
34.98
C


ATOM
8527
CG
PRO
A
538
75.435
−14.073
1.313
1.00
36.61
C


ATOM
8530
CD
PRO
A
538
75.002
−13.703
2.690
1.00
37.15
C


ATOM
8533
C
PRO
A
538
75.199
−17.199
3.117
1.00
33.85
C


ATOM
8534
O
PRO
A
538
75.684
−16.999
4.226
1.00
34.31
O


ATOM
8535
N
ILE
A
539
75.162
−18.386
2.529
1.00
32.85
N


ATOM
8536
CA
ILE
A
539
75.828
−19.562
3.077
1.00
32.36
C


ATOM
8538
CB
ILE
A
539
75.251
−20.854
2.449
1.00
31.82
C


ATOM
8540
CG1
ILE
A
539
73.789
−21.032
2.854
1.00
30.21
C


ATOM
8543
CD1
ILE
A
539
73.150
−22.267
2.262
1.00
32.80
C


ATOM
8547
CG2
ILE
A
539
76.042
−22.065
2.859
1.00
29.71
C


ATOM
8551
C
ILE
A
539
77.329
−19.452
2.794
1.00
32.68
C


ATOM
8552
O
ILE
A
539
77.729
−18.941
1.745
1.00
31.56
O


ATOM
8554
N
LEU
A
540
78.154
−19.914
3.734
1.00
34.01
N


ATOM
8555
CA
LEU
A
540
79.608
−19.813
3.586
1.00
35.36
C


ATOM
8557
CB
LEU
A
540
80.357
−20.383
4.806
1.00
35.94
C


ATOM
8560
CG
LEU
A
540
80.523
−19.526
6.069
1.00
38.17
C


ATOM
8562
CD1
LEU
A
540
81.512
−20.197
7.028
1.00
39.26
C


ATOM
8566
CD2
LEU
A
540
80.990
−18.113
5.742
1.00
39.10
C


ATOM
8570
C
LEU
A
540
80.044
−20.561
2.332
1.00
35.45
C


ATOM
8571
O
LEU
A
540
79.758
−21.748
2.206
1.00
34.76
O


ATOM
8573
N
PRO
A
541
80.745
−19.874
1.408
1.00
36.29
N


ATOM
8574
CA
PRO
A
541
81.115
−20.499
0.137
1.00
36.75
C


ATOM
8576
CB
PRO
A
541
82.013
−19.447
−0.532
1.00
36.53
C


ATOM
8579
CG
PRO
A
541
82.422
−18.532
0.551
1.00
36.20
C


ATOM
8582
CD
PRO
A
541
81.285
−18.507
1.508
1.00
36.11
C


ATOM
8585
C
PRO
A
541
81.861
−21.822
0.297
1.00
37.40
C


ATOM
8586
O
PRO
A
541
82.580
−22.018
1.275
1.00
37.11
O


ATOM
8587
N
PHE
A
542
81.669
−22.717
−0.667
1.00
38.70
N


ATOM
8588
CA
PHE
A
542
82.309
−24.028
−0.669
1.00
39.81
C


ATOM
8590
CB
PHE
A
542
81.910
−24.784
−1.944
1.00
38.81
C


ATOM
8593
CG
PHE
A
542
82.618
−26.094
−2.131
1.00
35.15
C


ATOM
8594
CD1
PHE
A
542
82.312
−27.184
−1.330
1.00
33.52
C


ATOM
8596
CE1
PHE
A
542
82.960
−28.403
−1.504
1.00
32.93
C


ATOM
8598
CZ
PHE
A
542
83.921
−28.540
−2.490
1.00
32.40
C


ATOM
8600
CE2
PHE
A
542
84.234
−27.457
−3.301
1.00
32.40
C


ATOM
8602
CD2
PHE
A
542
83.582
−26.243
−3.119
1.00
33.20
C


ATOM
8604
C
PHE
A
542
83.830
−23.894
−0.575
1.00
42.56
C


ATOM
8605
O
PHE
A
542
84.423
−23.060
−1.260
1.00
42.24
O


ATOM
8607
N
GLU
A
543
84.445
−24.699
0.291
1.00
46.36
N


ATOM
8608
CA
GLU
A
543
85.910
−24.774
0.414
1.00
49.96
C


ATOM
8610
CB
GLU
A
543
86.360
−24.329
1.815
1.00
50.34
C


ATOM
8613
CG
GLU
A
543
86.299
−22.818
2.046
1.00
52.01
C


ATOM
8616
CD
GLU
A
543
86.218
−22.443
3.521
1.00
54.80
C


ATOM
8617
OE1
GLU
A
543
85.219
−22.811
4.179
1.00
54.78
O


ATOM
8618
OE2
GLU
A
543
87.148
−21.771
4.018
1.00
54.99
O


ATOM
8619
C
GLU
A
543
86.392
−26.205
0.134
1.00
52.48
C


ATOM
8620
O
GLU
A
543
85.596
−27.147
0.148
1.00
53.17
O


ATOM
8622
N
ARG
A
544
87.691
−26.358
−0.126
1.00
55.15
N


ATOM
8623
CA
ARG
A
544
88.300
−27.677
−0.349
1.00
56.80
C


ATOM
8625
CB
ARG
A
544
88.913
−27.762
−1.755
1.00
57.52
C


ATOM
8628
CG
ARG
A
544
87.890
−27.687
−2.886
1.00
61.04
C


ATOM
8631
CD
ARG
A
544
88.551
−27.708
−4.270
1.00
66.09
C


ATOM
8634
NE
ARG
A
544
87.581
−27.496
−5.355
1.00
70.23
N


ATOM
8636
CZ
ARG
A
544
87.150
−26.307
−5.794
1.00
71.81
C


ATOM
8637
NH1
ARG
A
544
87.587
−25.170
−5.253
1.00
72.03
N


ATOM
8640
NH2
ARG
A
544
86.263
−26.251
−6.786
1.00
70.51
N


ATOM
8643
C
ARG
A
544
89.357
−27.961
0.723
1.00
57.03
C


ATOM
8644
O
ARG
A
544
89.018
−28.161
1.891
1.00
57.24
O


ATOM
8646
OXT
ARG
A
544
90.565
−27.989
0.467
1.00
57.03
O


ATOM
8647
N
SER
B
6
64.883
38.854
11.944
1.00
48.47
N


ATOM
8648
CA
SER
B
6
64.548
37.415
11.723
1.00
48.78
C


ATOM
8650
CB
SER
B
6
63.144
37.289
11.108
1.00
49.12
C


ATOM
8653
OG
SER
B
6
62.743
35.935
10.961
1.00
48.81
O


ATOM
8655
C
SER
B
6
65.606
36.778
10.815
1.00
48.64
C


ATOM
8656
O
SER
B
6
65.544
36.920
9.589
1.00
49.03
O


ATOM
8660
N
ALA
B
7
66.577
36.090
11.423
1.00
47.61
N


ATOM
8661
CA
ALA
B
7
67.705
35.498
10.687
1.00
47.15
C


ATOM
8663
CB
ALA
B
7
68.947
35.450
11.572
1.00
46.50
C


ATOM
8667
C
ALA
B
7
67.379
34.097
10.157
1.00
46.96
C


ATOM
8668
O
ALA
B
7
66.659
33.336
10.801
1.00
46.12
O


ATOM
8670
N
ASN
B
8
67.924
33.764
8.986
1.00
47.48
N


ATOM
8671
CA
ASN
B
8
67.707
32.453
8.365
1.00
48.21
C


ATOM
8673
CB
ASN
B
8
67.071
32.612
6.979
1.00
48.47
C


ATOM
8676
CG
ASN
B
8
66.763
31.273
6.314
1.00
50.21
C


ATOM
8677
OD1
ASN
B
8
66.453
30.285
6.985
1.00
49.98
O


ATOM
8678
ND2
ASN
B
8
66.844
31.240
4.987
1.00
50.79
N


ATOM
8681
C
ASN
B
8
68.997
31.639
8.242
1.00
48.33
C


ATOM
8682
O
ASN
B
8
69.946
32.061
7.576
1.00
48.66
O


ATOM
8684
N
TYR
B
9
69.017
30.475
8.891
1.00
48.45
N


ATOM
8685
CA
TYR
B
9
70.125
29.523
8.780
1.00
48.48
C


ATOM
8687
CB
TYR
B
9
70.836
29.375
10.128
1.00
47.96
C


ATOM
8690
CG
TYR
B
9
71.296
30.682
10.733
1.00
44.69
C


ATOM
8691
CD1
TYR
B
9
72.053
31.592
9.991
1.00
41.23
C


ATOM
8693
CE1
TYR
B
9
72.476
32.789
10.549
1.00
40.89
C


ATOM
8695
CZ
TYR
B
9
72.148
33.084
11.865
1.00
38.45
C


ATOM
8696
OH
TYR
B
9
72.562
34.261
12.441
1.00
36.53
O


ATOM
8698
CE2
TYR
B
9
71.406
32.199
12.616
1.00
37.50
C


ATOM
8700
CD2
TYR
B
9
70.988
31.005
12.053
1.00
40.50
C


ATOM
8702
C
TYR
B
9
69.653
28.148
8.297
1.00
49.85
C


ATOM
8703
O
TYR
B
9
70.414
27.177
8.344
1.00
49.70
O


ATOM
8705
N
GLU
B
10
68.404
28.068
7.830
1.00
51.51
N


ATOM
8706
CA
GLU
B
10
67.851
26.818
7.302
1.00
52.34
C


ATOM
8708
CB
GLU
B
10
66.328
26.919
7.127
1.00
52.98
C


ATOM
8711
CG
GLU
B
10
65.517
26.763
8.428
1.00
54.93
C


ATOM
8714
CD
GLU
B
10
64.829
25.401
8.555
1.00
59.05
C


ATOM
8715
OE1
GLU
B
10
64.239
24.928
7.554
1.00
58.28
O


ATOM
8716
OE2
GLU
B
10
64.863
24.812
9.661
1.00
59.68
O


ATOM
8717
C
GLU
B
10
68.520
26.506
5.964
1.00
52.09
C


ATOM
8718
O
GLU
B
10
68.645
27.398
5.119
1.00
51.52
O


ATOM
8720
N
PRO
B
11
68.955
25.243
5.766
1.00
52.25
N


ATOM
8721
CA
PRO
B
11
69.667
24.890
4.539
1.00
51.99
C


ATOM
8723
CB
PRO
B
11
70.325
23.556
4.897
1.00
51.92
C


ATOM
8726
CG
PRO
B
11
69.359
22.916
5.845
1.00
52.20
C


ATOM
8729
CD
PRO
B
11
68.682
24.050
6.596
1.00
52.52
C


ATOM
8732
C
PRO
B
11
68.706
24.708
3.366
1.00
51.28
C


ATOM
8733
O
PRO
B
11
67.663
24.071
3.521
1.00
51.73
O


ATOM
8734
N
ASN
B
12
69.053
25.272
2.212
1.00
50.08
N


ATOM
8735
CA
ASN
B
12
68.276
25.058
0.995
1.00
49.62
C


ATOM
8737
CB
ASN
B
12
68.717
26.028
−0.107
1.00
50.33
C


ATOM
8740
CG
ASN
B
12
68.497
27.485
0.264
1.00
51.11
C


ATOM
8741
OD1
ASN
B
12
67.361
27.942
0.396
1.00
52.95
O


ATOM
8742
ND2
ASN
B
12
69.588
28.226
0.415
1.00
52.33
N


ATOM
8745
C
ASN
B
12
68.417
23.619
0.490
1.00
48.51
C


ATOM
8746
O
ASN
B
12
69.419
22.952
0.749
1.00
47.86
O


ATOM
8748
N
SER
B
13
67.404
23.148
−0.231
1.00
48.33
N


ATOM
8749
CA
SER
B
13
67.445
21.828
−0.870
1.00
48.08
C


ATOM
8751
CB
SER
B
13
66.064
21.460
−1.436
1.00
47.79
C


ATOM
8754
OG
SER
B
13
65.432
22.584
−2.032
1.00
46.89
O


ATOM
8756
C
SER
B
13
68.513
21.756
−1.973
1.00
48.18
C


ATOM
8757
O
SER
B
13
69.066
20.686
−2.238
1.00
49.34
O


ATOM
8759
N
TRP
B
14
68.806
22.896
−2.599
1.00
47.36
N


ATOM
8760
CA
TRP
B
14
69.817
22.967
−3.662
1.00
46.99
C


ATOM
8762
CB
TRP
B
14
69.450
24.044
−4.700
1.00
47.52
C


ATOM
8765
CG
TRP
B
14
69.013
25.376
−4.144
1.00
49.89
C


ATOM
8766
CD1
TRP
B
14
67.729
25.824
−4.011
1.00
53.42
C


ATOM
8768
NE1
TRP
B
14
67.719
27.089
−3.472
1.00
55.27
N


ATOM
8770
CE2
TRP
B
14
69.012
27.487
−3.254
1.00
55.66
C


ATOM
8771
CD2
TRP
B
14
69.856
26.431
−3.667
1.00
51.66
C


ATOM
8772
CE3
TRP
B
14
71.241
26.587
−3.546
1.00
54.94
C


ATOM
8774
CZ3
TRP
B
14
71.739
27.781
−3.017
1.00
58.44
C


ATOM
8776
CH2
TRP
B
14
70.872
28.814
−2.616
1.00
60.63
C


ATOM
8778
CZ2
TRP
B
14
69.509
28.687
−2.729
1.00
59.38
C


ATOM
8780
C
TRP
B
14
71.258
23.173
−3.153
1.00
46.41
C


ATOM
8781
O
TRP
B
14
72.206
23.074
−3.934
1.00
46.06
O


ATOM
8783
N
ASP
B
15
71.428
23.445
−1.858
1.00
45.51
N


ATOM
8784
CA
ASP
B
15
72.766
23.592
−1.266
1.00
44.70
C


ATOM
8786
CB
ASP
B
15
72.678
23.776
0.262
1.00
45.12
C


ATOM
8789
CG
ASP
B
15
72.262
25.190
0.679
1.00
46.08
C


ATOM
8790
OD1
ASP
B
15
72.430
26.145
−0.115
1.00
48.86
O


ATOM
8791
OD2
ASP
B
15
71.783
25.344
1.825
1.00
46.92
O


ATOM
8792
C
ASP
B
15
73.660
22.384
−1.575
1.00
43.48
C


ATOM
8793
O
ASP
B
15
73.185
21.249
−1.618
1.00
43.00
O


ATOM
8795
N
TYR
B
16
74.954
22.640
−1.775
1.00
42.61
N


ATOM
8796
CA
TYR
B
16
75.926
21.588
−2.104
1.00
41.65
C


ATOM
8798
CB
TYR
B
16
77.252
22.201
−2.568
1.00
40.79
C


ATOM
8801
CG
TYR
B
16
77.187
22.928
−3.895
1.00
35.80
C


ATOM
8802
CD1
TYR
B
16
76.745
22.279
−5.047
1.00
30.88
C


ATOM
8804
CE1
TYR
B
16
76.695
22.940
−6.269
1.00
27.07
C


ATOM
8806
CZ
TYR
B
16
77.103
24.258
−6.355
1.00
27.18
C


ATOM
8807
OH
TYR
B
16
77.054
24.904
−7.574
1.00
22.85
O


ATOM
8809
CE2
TYR
B
16
77.561
24.921
−5.226
1.00
25.86
C


ATOM
8811
CD2
TYR
B
16
77.602
24.255
−4.007
1.00
28.91
C


ATOM
8813
C
TYR
B
16
76.206
20.623
−0.945
1.00
43.36
C


ATOM
8814
O
TYR
B
16
76.778
19.554
−1.161
1.00
42.69
O


ATOM
8816
N
ASP
B
17
75.842
21.016
0.277
1.00
45.64
N


ATOM
8817
CA
ASP
B
17
75.901
20.115
1.434
1.00
47.24
C


ATOM
8819
CB
ASP
B
17
75.721
20.892
2.754
1.00
47.11
C


ATOM
8822
CG
ASP
B
17
77.032
21.465
3.302
1.00
48.46
C


ATOM
8823
OD1
ASP
B
17
78.108
21.257
2.697
1.00
48.72
O


ATOM
8824
OD2
ASP
B
17
76.985
22.122
4.367
1.00
49.78
O


ATOM
8825
C
ASP
B
17
74.829
19.022
1.316
1.00
49.37
C


ATOM
8826
O
ASP
B
17
75.110
17.842
1.559
1.00
49.22
O


ATOM
8828
N
TYR
B
18
73.613
19.424
0.934
1.00
51.34
N


ATOM
8829
CA
TYR
B
18
72.478
18.502
0.789
1.00
53.17
C


ATOM
8831
CB
TYR
B
18
71.158
19.285
0.752
1.00
54.17
C


ATOM
8834
CG
TYR
B
18
69.908
18.425
0.805
1.00
60.93
C


ATOM
8835
CD1
TYR
B
18
69.363
18.023
2.029
1.00
66.26
C


ATOM
8837
CE1
TYR
B
18
68.206
17.235
2.084
1.00
68.59
C


ATOM
8839
CZ
TYR
B
18
67.583
16.848
0.905
1.00
70.46
C


ATOM
8840
OH
TYR
B
18
66.443
16.077
0.953
1.00
72.07
O


ATOM
8842
CE2
TYR
B
18
68.103
17.239
−0.325
1.00
68.49
C


ATOM
8844
CD2
TYR
B
18
69.257
18.027
−0.369
1.00
65.55
C


ATOM
8846
C
TYR
B
18
72.628
17.633
−0.463
1.00
53.15
C


ATOM
8847
O
TYR
B
18
72.673
16.406
−0.371
1.00
52.95
O


ATOM
8849
N
LEU
B
19
72.700
18.272
−1.631
1.00
54.03
N


ATOM
8850
CA
LEU
B
19
73.021
17.570
−2.879
1.00
54.66
C


ATOM
8852
CB
LEU
B
19
72.740
18.453
−4.101
1.00
54.30
C


ATOM
8855
CG
LEU
B
19
71.271
18.702
−4.471
1.00
53.94
C


ATOM
8857
CD1
LEU
B
19
71.146
19.852
−5.467
1.00
51.72
C


ATOM
8861
CD2
LEU
B
19
70.628
17.434
−5.028
1.00
53.44
C


ATOM
8865
C
LEU
B
19
74.497
17.224
−2.832
1.00
55.92
C


ATOM
8866
O
LEU
B
19
75.211
17.701
−1.953
1.00
57.53
O


ATOM
8868
N
LEU
B
20
74.962
16.388
−3.756
1.00
56.38
N


ATOM
8869
CA
LEU
B
20
76.402
16.155
−3.906
1.00
57.08
C


ATOM
8871
CB
LEU
B
20
77.081
17.498
−4.246
1.00
56.62
C


ATOM
8874
CG
LEU
B
20
78.138
17.576
−5.347
1.00
56.12
C


ATOM
8876
CD1
LEU
B
20
77.603
17.034
−6.666
1.00
54.24
C


ATOM
8880
CD2
LEU
B
20
78.606
19.019
−5.517
1.00
53.19
C


ATOM
8884
C
LEU
B
20
77.043
15.522
−2.643
1.00
58.82
C


ATOM
8885
O
LEU
B
20
78.253
15.659
−2.414
1.00
59.10
O


ATOM
8887
N
SER
B
21
76.234
14.821
−1.841
1.00
60.11
N


ATOM
8888
CA
SER
B
21
76.674
14.298
−0.538
1.00
60.95
C


ATOM
8890
CB
SER
B
21
75.613
14.577
0.530
1.00
61.07
C


ATOM
8893
OG
SER
B
21
76.125
14.354
1.834
1.00
59.55
O


ATOM
8895
C
SER
B
21
76.967
12.798
−0.596
1.00
61.73
C


ATOM
8896
O
SER
B
21
76.246
12.037
−1.241
1.00
62.23
O


ATOM
8898
N
ILE
B
28
82.844
12.022
−4.394
1.00
61.01
N


ATOM
8899
CA
ILE
B
28
81.876
13.095
−4.606
1.00
60.50
C


ATOM
8901
CB
ILE
B
28
80.417
12.542
−4.583
1.00
60.55
C


ATOM
8903
CG1
ILE
B
28
80.255
11.402
−5.599
1.00
61.10
C


ATOM
8906
CD1
ILE
B
28
78.832
10.870
−5.726
1.00
60.87
C


ATOM
8910
CG2
ILE
B
28
79.407
13.642
−4.884
1.00
59.57
C


ATOM
8914
C
ILE
B
28
82.033
14.219
−3.567
1.00
59.90
C


ATOM
8915
O
ILE
B
28
81.181
15.103
−3.479
1.00
60.41
O


ATOM
8917
N
GLU
B
29
83.127
14.200
−2.803
1.00
58.90
N


ATOM
8918
CA
GLU
B
29
83.319
15.151
−1.698
1.00
58.52
C


ATOM
8920
CB
GLU
B
29
83.679
14.411
−0.406
1.00
58.96
C


ATOM
8923
CG
GLU
B
29
82.494
13.674
0.216
1.00
61.50
C


ATOM
8926
CD
GLU
B
29
82.712
13.325
1.675
1.00
65.34
C


ATOM
8927
OE1
GLU
B
29
82.996
14.249
2.469
1.00
67.85
O


ATOM
8928
OE2
GLU
B
29
82.586
12.131
2.032
1.00
67.47
O


ATOM
8929
C
GLU
B
29
84.351
16.244
−1.996
1.00
57.31
C


ATOM
8930
O
GLU
B
29
84.126
17.408
−1.655
1.00
57.65
O


ATOM
8932
N
VAL
B
30
85.474
15.881
−2.616
1.00
55.38
N


ATOM
8933
CA
VAL
B
30
86.434
16.881
−3.117
1.00
53.51
C


ATOM
8935
CB
VAL
B
30
87.709
16.218
−3.704
1.00
53.54
C


ATOM
8937
CG1
VAL
B
30
88.466
17.184
−4.622
1.00
52.01
C


ATOM
8941
CG2
VAL
B
30
88.611
15.719
−2.576
1.00
53.28
C


ATOM
8945
C
VAL
B
30
85.756
17.752
−4.181
1.00
51.69
C


ATOM
8946
O
VAL
B
30
86.089
18.926
−4.354
1.00
51.90
O


ATOM
8948
N
TYR
B
31
84.808
17.145
−4.888
1.00
49.16
N


ATOM
8949
CA
TYR
B
31
83.926
17.825
−5.827
1.00
47.48
C


ATOM
8951
CB
TYR
B
31
83.041
16.759
−6.481
1.00
48.12
C


ATOM
8954
CG
TYR
B
31
82.324
17.145
−7.749
1.00
51.51
C


ATOM
8955
CD1
TYR
B
31
82.972
17.108
−8.985
1.00
55.27
C


ATOM
8957
CE1
TYR
B
31
82.297
17.439
−10.162
1.00
58.69
C


ATOM
8959
CZ
TYR
B
31
80.948
17.792
−10.106
1.00
61.93
C


ATOM
8960
OH
TYR
B
31
80.249
18.124
−11.251
1.00
60.06
O


ATOM
8962
CE2
TYR
B
31
80.285
17.814
−8.888
1.00
61.49
C


ATOM
8964
CD2
TYR
B
31
80.975
17.484
−7.721
1.00
57.69
C


ATOM
8966
C
TYR
B
31
83.074
18.867
−5.088
1.00
44.90
C


ATOM
8967
O
TYR
B
31
82.977
20.018
−5.512
1.00
43.94
O


ATOM
8969
N
LYS
B
32
82.486
18.445
−3.970
1.00
42.56
N


ATOM
8970
CA
LYS
B
32
81.599
19.278
−3.141
1.00
40.47
C


ATOM
8972
CB
LYS
B
32
80.934
18.409
−2.054
1.00
40.96
C


ATOM
8975
CG
LYS
B
32
80.533
19.121
−0.751
1.00
41.25
C


ATOM
8978
CD
LYS
B
32
80.081
18.110
0.311
1.00
42.15
C


ATOM
8981
CE
LYS
B
32
80.368
18.587
1.733
1.00
43.11
C


ATOM
8984
NZ
LYS
B
32
80.378
17.457
2.713
1.00
41.98
N


ATOM
8988
C
LYS
B
32
82.301
20.475
−2.500
1.00
38.14
C


ATOM
8989
O
LYS
B
32
81.766
21.581
−2.506
1.00
37.75
O


ATOM
8991
N
ASP
B
33
83.481
20.248
−1.930
1.00
35.83
N


ATOM
8992
CA
ASP
B
33
84.219
21.316
−1.239
1.00
34.43
C


ATOM
8994
CB
ASP
B
33
85.416
20.748
−0.464
1.00
35.02
C


ATOM
8997
CG
ASP
B
33
84.997
20.030
0.810
1.00
37.63
C


ATOM
8998
OD1
ASP
B
33
84.337
20.672
1.661
1.00
41.48
O


ATOM
8999
OD2
ASP
B
33
85.328
18.831
0.960
1.00
38.68
O


ATOM
9000
C
ASP
B
33
84.684
22.409
−2.194
1.00
31.96
C


ATOM
9001
O
ASP
B
33
84.751
23.578
−1.814
1.00
31.22
O


ATOM
9003
N
LYS
B
34
84.997
22.018
−3.427
1.00
29.68
N


ATOM
9004
CA
LYS
B
34
85.373
22.959
−4.474
1.00
28.34
C


ATOM
9006
CB
LYS
B
34
85.877
22.211
−5.702
1.00
28.95
C


ATOM
9009
CG
LYS
B
34
86.675
23.073
−6.661
1.00
32.79
C


ATOM
9012
CD
LYS
B
34
87.396
22.216
−7.690
1.00
37.60
C


ATOM
9015
CE
LYS
B
34
88.828
22.670
−7.896
1.00
38.74
C


ATOM
9018
NZ
LYS
B
34
89.579
21.684
−8.723
1.00
40.14
N


ATOM
9022
C
LYS
B
34
84.193
23.833
−4.872
1.00
25.98
C


ATOM
9023
O
LYS
B
34
84.334
25.044
−5.011
1.00
26.13
O


ATOM
9025
N
ALA
B
35
83.036
23.207
−5.056
1.00
23.61
N


ATOM
9026
CA
ALA
B
35
81.813
23.912
−5.423
1.00
21.89
C


ATOM
9028
CB
ALA
B
35
80.662
22.923
−5.583
1.00
21.48
C


ATOM
9032
C
ALA
B
35
81.467
24.962
−4.374
1.00
20.57
C


ATOM
9033
O
ALA
B
35
81.184
26.113
−4.705
1.00
20.10
O


ATOM
9035
N
LYS
B
36
81.493
24.551
−3.111
1.00
19.17
N


ATOM
9036
CA
LYS
B
36
81.265
25.457
−1.992
1.00
18.70
C


ATOM
9038
CB
LYS
B
36
81.391
24.705
−0.655
1.00
18.72
C


ATOM
9041
CG
LYS
B
36
80.136
23.913
−0.283
1.00
20.73
C


ATOM
9044
CD
LYS
B
36
80.423
22.712
0.623
1.00
22.10
C


ATOM
9047
CE
LYS
B
36
80.652
23.097
2.077
1.00
21.54
C


ATOM
9050
NZ
LYS
B
36
81.251
21.958
2.849
1.00
20.74
N


ATOM
9054
C
LYS
B
36
82.232
26.641
−2.025
1.00
18.21
C


ATOM
9055
O
LYS
B
36
81.818
27.794
−1.835
1.00
18.06
O


ATOM
9057
N
LYS
B
37
83.510
26.348
−2.269
1.00
17.44
N


ATOM
9058
CA
LYS
B
37
84.559
27.368
−2.321
1.00
17.41
C


ATOM
9060
CB
LYS
B
37
85.935
26.698
−2.442
1.00
18.08
C


ATOM
9063
CG
LYS
B
37
87.138
27.639
−2.366
1.00
22.32
C


ATOM
9066
CD
LYS
B
37
88.429
26.837
−2.154
1.00
29.04
C


ATOM
9069
CE
LYS
B
37
89.661
27.721
−1.954
1.00
30.38
C


ATOM
9072
NZ
LYS
B
37
90.117
28.346
−3.226
1.00
32.86
N


ATOM
9076
C
LYS
B
37
84.328
28.338
−3.481
1.00
15.98
C


ATOM
9077
O
LYS
B
37
84.416
29.543
−3.305
1.00
16.55
O


ATOM
9079
N
LEU
B
38
84.014
27.808
−4.659
1.00
14.47
N


ATOM
9080
CA
LEU
B
38
83.742
28.643
−5.831
1.00
13.74
C


ATOM
9082
CB
LEU
B
38
83.618
27.780
−7.093
1.00
13.39
C


ATOM
9085
CG
LEU
B
38
84.842
26.938
−7.477
1.00
12.68
C


ATOM
9087
CD1
LEU
B
38
84.482
25.960
−8.593
1.00
8.31
C


ATOM
9091
CD2
LEU
B
38
86.024
27.823
−7.864
1.00
8.06
C


ATOM
9095
C
LEU
B
38
82.472
29.480
−5.641
1.00
13.46
C


ATOM
9096
O
LEU
B
38
82.472
30.685
−5.901
1.00
12.46
O


ATOM
9098
N
GLU
B
39
81.396
28.826
−5.200
1.00
13.50
N


ATOM
9099
CA
GLU
B
39
80.141
29.497
−4.875
1.00
13.46
C


ATOM
9101
CB
GLU
B
39
79.177
28.519
−4.211
1.00
13.91
C


ATOM
9104
CG
GLU
B
39
77.793
29.091
−3.923
1.00
17.09
C


ATOM
9107
CD
GLU
B
39
76.833
28.076
−3.300
1.00
18.78
C


ATOM
9108
OE1
GLU
B
39
77.304
27.126
−2.631
1.00
16.35
O


ATOM
9109
OE2
GLU
B
39
75.600
28.248
−3.467
1.00
18.53
O


ATOM
9110
C
GLU
B
39
80.382
30.684
−3.948
1.00
13.70
C


ATOM
9111
O
GLU
B
39
79.900
31.789
−4.208
1.00
14.66
O


ATOM
9113
N
ALA
B
40
81.138
30.453
−2.878
1.00
13.12
N


ATOM
9114
CA
ALA
B
40
81.488
31.509
−1.927
1.00
12.47
C


ATOM
9116
CB
ALA
B
40
82.497
30.986
−0.895
1.00
11.95
C


ATOM
9120
C
ALA
B
40
82.044
32.746
−2.623
1.00
11.90
C


ATOM
9121
O
ALA
B
40
81.578
33.862
−2.389
1.00
12.67
O


ATOM
9123
N
GLU
B
41
83.034
32.543
−3.484
1.00
11.40
N


ATOM
9124
CA
GLU
B
41
83.698
33.650
−4.167
1.00
11.33
C


ATOM
9126
CB
GLU
B
41
84.852
33.127
−5.022
1.00
12.06
C


ATOM
9129
CG
GLU
B
41
85.802
34.207
−5.532
1.00
15.88
C


ATOM
9132
CD
GLU
B
41
87.145
33.650
−6.011
1.00
20.21
C


ATOM
9133
OE1
GLU
B
41
87.643
32.661
−5.423
1.00
21.00
O


ATOM
9134
OE2
GLU
B
41
87.709
34.217
−6.972
1.00
22.71
O


ATOM
9135
C
GLU
B
41
82.720
34.460
−5.018
1.00
10.64
C


ATOM
9136
O
GLU
B
41
82.741
35.679
−4.983
1.00
11.27
O


ATOM
9138
N
VAL
B
42
81.848
33.789
−5.762
1.00
10.57
N


ATOM
9139
CA
VAL
B
42
80.860
34.486
−6.586
1.00
10.29
C


ATOM
9141
CB
VAL
B
42
80.024
33.519
−7.442
1.00
9.81
C


ATOM
9143
CG1
VAL
B
42
78.897
34.270
−8.119
1.00
8.26
C


ATOM
9147
CG2
VAL
B
42
80.893
32.815
−8.480
1.00
6.04
C


ATOM
9151
C
VAL
B
42
79.921
35.320
−5.718
1.00
12.18
C


ATOM
9152
O
VAL
B
42
79.659
36.479
−6.025
1.00
13.93
O


ATOM
9154
N
ARG
B
43
79.424
34.732
−4.631
1.00
13.43
N


ATOM
9155
CA
ARG
B
43
78.572
35.450
−3.678
1.00
13.22
C


ATOM
9157
CB
ARG
B
43
78.221
34.555
−2.485
1.00
13.74
C


ATOM
9160
CG
ARG
B
43
77.471
35.270
−1.337
1.00
16.34
C


ATOM
9163
CD
ARG
B
43
78.427
35.901
−0.294
1.00
17.10
C


ATOM
9166
NE
ARG
B
43
77.708
36.654
0.734
1.00
15.55
N


ATOM
9168
CZ
ARG
B
43
78.258
37.538
1.566
1.00
12.32
C


ATOM
9169
NH1
ARG
B
43
77.496
38.164
2.452
1.00
12.93
N


ATOM
9172
NH2
ARG
B
43
79.557
37.813
1.521
1.00
15.37
N


ATOM
9175
C
ARG
B
43
79.252
36.723
−3.188
1.00
13.14
C


ATOM
9176
O
ARG
B
43
78.620
37.780
−3.106
1.00
12.59
O


ATOM
9178
N
ARG
B
44
80.535
36.612
−2.849
1.00
13.11
N


ATOM
9179
CA
ARG
B
44
81.314
37.770
−2.422
1.00
12.87
C


ATOM
9181
CB
ARG
B
44
82.766
37.385
−2.142
1.00
12.31
C


ATOM
9184
CG
ARG
B
44
83.574
38.495
−1.490
1.00
12.75
C


ATOM
9187
CD
ARG
B
44
85.021
38.097
−1.274
1.00
11.37
C


ATOM
9190
NE
ARG
B
44
85.677
37.787
−2.537
1.00
11.31
N


ATOM
9192
CZ
ARG
B
44
86.840
37.155
−2.662
1.00
12.26
C


ATOM
9193
NH1
ARG
B
44
87.519
36.743
−1.596
1.00
13.75
N


ATOM
9196
NH2
ARG
B
44
87.324
36.929
−3.875
1.00
12.62
N


ATOM
9199
C
ARG
B
44
81.282
38.861
−3.484
1.00
13.22
C


ATOM
9200
O
ARG
B
44
81.045
40.029
−3.175
1.00
13.74
O


ATOM
9202
N
GLU
B
45
81.515
38.477
−4.735
1.00
13.28
N


ATOM
9203
CA
GLU
B
45
81.644
39.452
−5.811
1.00
12.72
C


ATOM
9205
CB
GLU
B
45
82.265
38.817
−7.055
1.00
13.40
C


ATOM
9208
CG
GLU
B
45
83.717
38.364
−6.861
1.00
14.06
C


ATOM
9211
CD
GLU
B
45
84.634
39.491
−6.420
1.00
16.74
C


ATOM
9212
OE1
GLU
B
45
84.613
40.557
−7.075
1.00
21.90
O


ATOM
9213
OE2
GLU
B
45
85.370
39.314
−5.420
1.00
13.76
O


ATOM
9214
C
GLU
B
45
80.306
40.093
−6.125
1.00
12.58
C


ATOM
9215
O
GLU
B
45
80.244
41.283
−6.397
1.00
12.39
O


ATOM
9217
N
ILE
B
46
79.229
39.319
−6.052
1.00
13.42
N


ATOM
9218
CA
ILE
B
46
77.893
39.878
−6.242
1.00
13.38
C


ATOM
9220
CB
ILE
B
46
76.803
38.779
−6.316
1.00
14.14
C


ATOM
9222
CG1
ILE
B
46
77.038
37.858
−7.521
1.00
13.45
C


ATOM
9225
CD1
ILE
B
46
76.166
36.623
−7.527
1.00
9.11
C


ATOM
9229
CG2
ILE
B
46
75.408
39.405
−6.412
1.00
10.01
C


ATOM
9233
C
ILE
B
46
77.571
40.879
−5.124
1.00
13.50
C


ATOM
9234
O
ILE
B
46
76.929
41.896
−5.374
1.00
15.08
O


ATOM
9236
N
ASN
B
47
78.041
40.602
−3.905
1.00
12.99
N


ATOM
9237
CA
ASN
B
47
77.787
41.477
−2.750
1.00
12.52
C


ATOM
9239
CB
ASN
B
47
77.773
40.663
−1.454
1.00
12.54
C


ATOM
9242
CG
ASN
B
47
76.471
39.917
−1.249
1.00
12.61
C


ATOM
9243
OD1
ASN
B
47
75.498
40.478
−0.755
1.00
15.74
O


ATOM
9244
ND2
ASN
B
47
76.452
38.645
−1.620
1.00
7.01
N


ATOM
9247
C
ASN
B
47
78.763
42.647
−2.612
1.00
12.79
C


ATOM
9248
O
ASN
B
47
78.590
43.503
−1.745
1.00
13.08
O


ATOM
9250
N
ASN
B
48
79.776
42.692
−3.471
1.00
13.46
N


ATOM
9251
CA
ASN
B
48
80.757
43.776
−3.465
1.00
13.69
C


ATOM
9253
CB
ASN
B
48
81.633
43.689
−4.724
1.00
13.85
C


ATOM
9256
CG
ASN
B
48
82.776
44.691
−4.724
1.00
12.53
C


ATOM
9257
OD1
ASN
B
48
83.054
45.347
−3.723
1.00
10.59
O


ATOM
9258
ND2
ASN
B
48
83.439
44.814
−5.861
1.00
8.19
N


ATOM
9261
C
ASN
B
48
80.087
45.143
−3.386
1.00
13.86
C


ATOM
9262
O
ASN
B
48
79.442
45.573
−4.326
1.00
14.56
O


ATOM
9264
N
GLU
B
49
80.256
45.822
−2.261
1.00
15.14
N


ATOM
9265
CA
GLU
B
49
79.594
47.101
−2.025
1.00
16.03
C


ATOM
9267
CB
GLU
B
49
79.592
47.438
−0.529
1.00
16.78
C


ATOM
9270
CG
GLU
B
49
78.891
46.411
0.361
1.00
16.28
C


ATOM
9273
CD
GLU
B
49
79.834
45.362
0.949
1.00
20.23
C


ATOM
9274
OE1
GLU
B
49
80.995
45.249
0.486
1.00
17.56
O


ATOM
9275
OE2
GLU
B
49
79.403
44.645
1.883
1.00
19.62
O


ATOM
9276
C
GLU
B
49
80.211
48.271
−2.800
1.00
16.41
C


ATOM
9277
O
GLU
B
49
79.580
49.312
−2.921
1.00
16.61
O


ATOM
9279
N
LYS
B
50
81.439
48.109
−3.294
1.00
16.71
N


ATOM
9280
CA
LYS
B
50
82.107
49.140
−4.097
1.00
17.09
C


ATOM
9282
CB
LYS
B
50
83.452
49.554
−3.466
1.00
17.11
C


ATOM
9285
CG
LYS
B
50
83.335
50.329
−2.157
1.00
19.17
C


ATOM
9288
CD
LYS
B
50
84.590
51.151
−1.875
1.00
21.81
C


ATOM
9291
CE
LYS
B
50
84.498
51.902
−0.555
1.00
23.59
C


ATOM
9294
NZ
LYS
B
50
84.350
50.998
0.615
1.00
21.79
N


ATOM
9298
C
LYS
B
50
82.338
48.649
−5.523
1.00
16.99
C


ATOM
9299
O
LYS
B
50
83.294
49.060
−6.185
1.00
17.71
O


ATOM
9301
N
ALA
B
51
81.461
47.776
−5.998
1.00
17.34
N


ATOM
9302
CA
ALA
B
51
81.572
47.244
−7.353
1.00
18.17
C


ATOM
9304
CB
ALA
B
51
80.598
46.077
−7.558
1.00
16.96
C


ATOM
9308
C
ALA
B
51
81.297
48.341
−8.372
1.00
18.69
C


ATOM
9309
O
ALA
B
51
80.430
49.191
−8.162
1.00
17.88
O


ATOM
9311
N
GLU
B
52
82.041
48.317
−9.472
1.00
20.08
N


ATOM
9312
CA
GLU
B
52
81.792
49.231
−10.581
1.00
21.52
C


ATOM
9314
CB
GLU
B
52
82.901
49.118
−11.635
1.00
22.96
C


ATOM
9317
CG
GLU
B
52
83.356
50.463
−12.221
1.00
30.88
C


ATOM
9320
CD
GLU
B
52
84.392
51.200
−11.358
1.00
38.65
C


ATOM
9321
OE1
GLU
B
52
84.620
50.803
−10.192
1.00
40.61
O


ATOM
9322
OE2
GLU
B
52
84.979
52.190
−11.854
1.00
43.64
O


ATOM
9323
C
GLU
B
52
80.425
48.879
−11.175
1.00
20.30
C


ATOM
9324
O
GLU
B
52
80.070
47.708
−11.258
1.00
19.47
O


ATOM
9326
N
PHE
B
53
79.658
49.887
−11.578
1.00
20.29
N


ATOM
9327
CA
PHE
B
53
78.264
49.668
−11.986
1.00
20.32
C


ATOM
9329
CB
PHE
B
53
77.560
50.990
−12.310
1.00
20.20
C


ATOM
9332
CG
PHE
B
53
77.380
51.903
−11.124
1.00
21.60
C


ATOM
9333
CD1
PHE
B
53
77.052
51.401
−9.868
1.00
20.90
C


ATOM
9335
CE1
PHE
B
53
76.885
52.254
−8.782
1.00
21.56
C


ATOM
9337
CZ
PHE
B
53
77.024
53.617
−8.948
1.00
22.59
C


ATOM
9339
CE2
PHE
B
53
77.342
54.131
−10.197
1.00
21.70
C


ATOM
9341
CD2
PHE
B
53
77.513
53.278
−11.276
1.00
21.01
C


ATOM
9343
C
PHE
B
53
78.117
48.723
−13.174
1.00
20.26
C


ATOM
9344
O
PHE
B
53
77.372
47.749
−13.103
1.00
21.14
O


ATOM
9346
N
LEU
B
54
78.820
49.011
−14.260
1.00
20.40
N


ATOM
9347
CA
LEU
B
54
78.700
48.208
−15.472
1.00
20.90
C


ATOM
9349
CB
LEU
B
54
79.411
48.894
−16.647
1.00
21.53
C


ATOM
9352
CG
LEU
B
54
78.824
50.246
−17.072
1.00
24.00
C


ATOM
9354
CD1
LEU
B
54
79.749
50.975
−18.050
1.00
26.24
C


ATOM
9358
CD2
LEU
B
54
77.438
50.069
−17.673
1.00
21.58
C


ATOM
9362
C
LEU
B
54
79.243
46.793
−15.251
1.00
20.35
C


ATOM
9363
O
LEU
B
54
78.685
45.818
−15.763
1.00
21.81
O


ATOM
9365
N
THR
B
55
80.313
46.682
−14.472
1.00
18.78
N


ATOM
9366
CA
THR
B
55
80.917
45.383
−14.179
1.00
18.03
C


ATOM
9368
CB
THR
B
55
82.299
45.559
−13.520
1.00
17.23
C


ATOM
9370
OG1
THR
B
55
83.121
46.326
−14.399
1.00
18.09
O


ATOM
9372
CG2
THR
B
55
82.963
44.218
−13.259
1.00
15.26
C


ATOM
9376
C
THR
B
55
80.013
44.502
−13.308
1.00
16.91
C


ATOM
9377
O
THR
B
55
79.960
43.291
−13.505
1.00
16.12
O


ATOM
9379
N
LEU
B
56
79.308
45.113
−12.361
1.00
15.75
N


ATOM
9380
CA
LEU
B
56
78.365
44.388
−11.515
1.00
15.83
C


ATOM
9382
CB
LEU
B
56
77.834
45.299
−10.403
1.00
16.00
C


ATOM
9385
CG
LEU
B
56
76.761
44.754
−9.451
1.00
16.57
C


ATOM
9387
CD1
LEU
B
56
77.269
43.576
−8.630
1.00
11.81
C


ATOM
9391
CD2
LEU
B
56
76.265
45.875
−8.546
1.00
16.49
C


ATOM
9395
C
LEU
B
56
77.206
43.838
−12.348
1.00
16.22
C


ATOM
9396
O
LEU
B
56
76.833
42.674
−12.206
1.00
15.75
O


ATOM
9398
N
LEU
B
57
76.640
44.679
−13.214
1.00
16.11
N


ATOM
9399
CA
LEU
B
57
75.524
44.263
−14.060
1.00
15.93
C


ATOM
9401
CB
LEU
B
57
74.981
45.429
−14.892
1.00
15.36
C


ATOM
9404
CG
LEU
B
57
74.372
46.615
−14.132
1.00
14.37
C


ATOM
9406
CD1
LEU
B
57
74.113
47.771
−15.081
1.00
11.63
C


ATOM
9410
CD2
LEU
B
57
73.100
46.241
−13.394
1.00
6.32
C


ATOM
9414
C
LEU
B
57
75.960
43.132
−14.981
1.00
17.19
C


ATOM
9415
O
LEU
B
57
75.233
42.155
−15.154
1.00
17.60
O


ATOM
9417
N
GLU
B
58
77.150
43.257
−15.566
1.00
18.01
N


ATOM
9418
CA
GLU
B
58
77.672
42.200
−16.440
1.00
18.65
C


ATOM
9420
CB
GLU
B
58
78.968
42.632
−17.135
1.00
18.84
C


ATOM
9423
CG
GLU
B
58
78.753
43.535
−18.344
1.00
24.47
C


ATOM
9426
CD
GLU
B
58
80.058
44.038
−18.945
1.00
30.72
C


ATOM
9427
OE1
GLU
B
58
81.133
43.532
−18.554
1.00
36.44
O


ATOM
9428
OE2
GLU
B
58
80.007
44.940
−19.811
1.00
35.35
O


ATOM
9429
C
GLU
B
58
77.899
40.911
−15.656
1.00
17.82
C


ATOM
9430
O
GLU
B
58
77.599
39.818
−16.146
1.00
18.63
O


ATOM
9432
N
LEU
B
59
78.429
41.043
−14.443
1.00
16.80
N


ATOM
9433
CA
LEU
B
59
78.659
39.888
−13.589
1.00
16.40
C


ATOM
9435
CB
LEU
B
59
79.329
40.290
−12.264
1.00
16.00
C


ATOM
9438
CG
LEU
B
59
79.346
39.240
−11.145
1.00
15.18
C


ATOM
9440
CD1
LEU
B
59
79.890
37.921
−11.659
1.00
10.29
C


ATOM
9444
CD2
LEU
B
59
80.146
39.728
−9.950
1.00
10.60
C


ATOM
9448
C
LEU
B
59
77.327
39.189
−13.341
1.00
15.64
C


ATOM
9449
O
LEU
B
59
77.215
37.975
−13.532
1.00
16.67
O


ATOM
9451
N
ILE
B
60
76.318
39.962
−12.954
1.00
13.99
N


ATOM
9452
CA
ILE
B
60
75.012
39.403
−12.666
1.00
13.53
C


ATOM
9454
CB
ILE
B
60
74.025
40.468
−12.146
1.00
13.60
C


ATOM
9456
CG1
ILE
B
60
74.458
40.979
−10.765
1.00
12.49
C


ATOM
9459
CD1
ILE
B
60
73.702
42.213
−10.308
1.00
12.82
C


ATOM
9463
CG2
ILE
B
60
72.632
39.889
−12.024
1.00
10.70
C


ATOM
9467
C
ILE
B
60
74.450
38.712
−13.903
1.00
13.93
C


ATOM
9468
O
ILE
B
60
73.940
37.595
−13.816
1.00
14.15
O


ATOM
9470
N
ASP
B
61
74.569
39.363
−15.055
1.00
14.81
N


ATOM
9471
CA
ASP
B
61
74.071
38.801
−16.315
1.00
14.75
C


ATOM
9473
CB
ASP
B
61
74.324
39.772
−17.482
1.00
14.89
C


ATOM
9476
CG
ASP
B
61
73.563
39.394
−18.753
1.00
15.23
C


ATOM
9477
OD1
ASP
B
61
72.482
38.777
−18.663
1.00
18.70
O


ATOM
9478
OD2
ASP
B
61
74.040
39.734
−19.852
1.00
18.00
O


ATOM
9479
C
ASP
B
61
74.736
37.457
−16.602
1.00
14.52
C


ATOM
9480
O
ASP
B
61
74.064
36.490
−16.959
1.00
13.91
O


ATOM
9482
N
ASN
B
62
76.054
37.401
−16.437
1.00
14.06
N


ATOM
9483
CA
ASN
B
62
76.791
36.178
−16.711
1.00
14.95
C


ATOM
9485
CB
ASN
B
62
78.295
36.429
−16.626
1.00
15.37
C


ATOM
9488
CG
ASN
B
62
78.816
37.190
−17.821
1.00
16.57
C


ATOM
9489
OD1
ASN
B
62
78.345
36.994
−18.949
1.00
14.84
O


ATOM
9490
ND2
ASN
B
62
79.791
38.068
−17.587
1.00
15.38
N


ATOM
9493
C
ASN
B
62
76.389
35.053
−15.771
1.00
15.15
C


ATOM
9494
O
ASN
B
62
76.118
33.932
−16.214
1.00
15.85
O


ATOM
9496
N
VAL
B
63
76.336
35.360
−14.477
1.00
14.69
N


ATOM
9497
CA
VAL
B
63
75.876
34.394
−13.487
1.00
13.71
C


ATOM
9499
CB
VAL
B
63
75.739
35.019
−12.077
1.00
14.21
C


ATOM
9501
CG1
VAL
B
63
74.972
34.071
−11.116
1.00
11.92
C


ATOM
9505
CG2
VAL
B
63
77.099
35.365
−11.525
1.00
9.30
C


ATOM
9509
C
VAL
B
63
74.552
33.794
−13.920
1.00
12.66
C


ATOM
9510
O
VAL
B
63
74.412
32.582
−13.930
1.00
12.79
O


ATOM
9512
N
GLN
B
64
73.600
34.641
−14.305
1.00
13.68
N


ATOM
9513
CA
GLN
B
64
72.258
34.177
−14.709
1.00
14.66
C


ATOM
9515
CB
GLN
B
64
71.266
35.352
−14.830
1.00
13.97
C


ATOM
9518
CG
GLN
B
64
70.994
36.047
−13.495
1.00
13.24
C


ATOM
9521
CD
GLN
B
64
69.708
36.856
−13.454
1.00
13.96
C


ATOM
9522
OE1
GLN
B
64
69.040
36.911
−12.421
1.00
18.65
O


ATOM
9523
NE2
GLN
B
64
69.362
37.493
−14.562
1.00
11.65
N


ATOM
9526
C
GLN
B
64
72.292
33.361
−16.003
1.00
15.39
C


ATOM
9527
O
GLN
B
64
71.722
32.266
−16.067
1.00
14.79
O


ATOM
9529
N
ARG
B
65
72.974
33.881
−17.020
1.00
16.01
N


ATOM
9530
CA
ARG
B
65
73.067
33.186
−18.302
1.00
16.84
C


ATOM
9532
CB
ARG
B
65
73.740
34.059
−19.360
1.00
17.41
C


ATOM
9535
CG
ARG
B
65
72.895
35.256
−19.778
1.00
18.72
C


ATOM
9538
CD
ARG
B
65
73.497
35.987
−20.947
1.00
19.71
C


ATOM
9541
NE
ARG
B
65
73.369
35.221
−22.182
1.00
26.28
N


ATOM
9543
CZ
ARG
B
65
73.811
35.622
−23.374
1.00
30.59
C


ATOM
9544
NH1
ARG
B
65
74.421
36.799
−23.511
1.00
27.82
N


ATOM
9547
NH2
ARG
B
65
73.642
34.838
−24.436
1.00
32.71
N


ATOM
9550
C
ARG
B
65
73.782
31.842
−18.191
1.00
17.09
C


ATOM
9551
O
ARG
B
65
73.387
30.874
−18.855
1.00
17.32
O


ATOM
9553
N
LEU
B
66
74.809
31.770
−17.341
1.00
15.97
N


ATOM
9554
CA
LEU
B
66
75.549
30.517
−17.153
1.00
15.00
C


ATOM
9556
CB
LEU
B
66
76.893
30.772
−16.453
1.00
14.70
C


ATOM
9559
CG
LEU
B
66
77.952
31.521
−17.283
1.00
12.62
C


ATOM
9561
CD1
LEU
B
66
79.057
32.081
−16.391
1.00
8.94
C


ATOM
9565
CD2
LEU
B
66
78.533
30.634
−18.366
1.00
7.32
C


ATOM
9569
C
LEU
B
66
74.731
29.449
−16.406
1.00
14.71
C


ATOM
9570
O
LEU
B
66
75.162
28.306
−16.288
1.00
14.62
O


ATOM
9572
N
GLY
B
67
73.557
29.820
−15.903
1.00
14.95
N


ATOM
9573
CA
GLY
B
67
72.635
28.853
−15.300
1.00
15.26
C


ATOM
9576
C
GLY
B
67
72.611
28.867
−13.785
1.00
15.44
C


ATOM
9577
O
GLY
B
67
71.950
28.028
−13.176
1.00
16.14
O


ATOM
9579
N
LEU
B
68
73.304
29.831
−13.178
1.00
14.71
N


ATOM
9580
CA
LEU
B
68
73.492
29.869
−11.725
1.00
14.50
C


ATOM
9582
CB
LEU
B
68
74.936
30.262
−11.394
1.00
14.16
C


ATOM
9585
CG
LEU
B
68
76.016
29.288
−11.860
1.00
13.08
C


ATOM
9587
CD1
LEU
B
68
77.371
29.948
−11.787
1.00
8.19
C


ATOM
9591
CD2
LEU
B
68
75.976
28.020
−11.013
1.00
11.07
C


ATOM
9595
C
LEU
B
68
72.552
30.838
−11.016
1.00
14.81
C


ATOM
9596
O
LEU
B
68
72.589
30.943
−9.797
1.00
15.06
O


ATOM
9598
N
GLY
B
69
71.715
31.544
−11.768
1.00
15.08
N


ATOM
9599
CA
GLY
B
69
70.816
32.539
−11.191
1.00
14.97
C


ATOM
9602
C
GLY
B
69
70.007
32.042
−10.006
1.00
14.72
C


ATOM
9603
O
GLY
B
69
69.908
32.729
−8.998
1.00
15.67
O


ATOM
9605
N
TYR
B
70
69.437
30.847
−10.122
1.00
15.09
N


ATOM
9606
CA
TYR
B
70
68.586
30.273
−9.065
1.00
15.84
C


ATOM
9608
CB
TYR
B
70
68.073
28.886
−9.481
1.00
15.07
C


ATOM
9611
CG
TYR
B
70
69.095
27.776
−9.344
1.00
13.28
C


ATOM
9612
CD1
TYR
B
70
70.078
27.583
−10.309
1.00
10.92
C


ATOM
9614
CE1
TYR
B
70
71.020
26.571
−10.183
1.00
12.66
C


ATOM
9616
CZ
TYR
B
70
70.982
25.740
−9.077
1.00
15.33
C


ATOM
9617
OH
TYR
B
70
71.909
24.740
−8.929
1.00
13.26
O


ATOM
9619
CE2
TYR
B
70
70.014
25.916
−8.106
1.00
12.99
C


ATOM
9621
CD2
TYR
B
70
69.084
26.928
−8.241
1.00
11.29
C


ATOM
9623
C
TYR
B
70
69.302
30.154
−7.723
1.00
17.70
C


ATOM
9624
O
TYR
B
70
68.670
30.085
−6.678
1.00
21.15
O


ATOM
9626
N
ARG
B
71
70.624
30.126
−7.768
1.00
17.91
N


ATOM
9627
CA
ARG
B
71
71.448
29.851
−6.609
1.00
18.30
C


ATOM
9629
CB
ARG
B
71
72.646
29.024
−7.083
1.00
18.32
C


ATOM
9632
CG
ARG
B
71
73.780
28.859
−6.108
1.00
20.58
C


ATOM
9635
CD
ARG
B
71
74.748
27.803
−6.612
1.00
20.77
C


ATOM
9638
NE
ARG
B
71
74.081
26.514
−6.729
1.00
22.51
N


ATOM
9640
CZ
ARG
B
71
73.903
25.655
−5.731
1.00
23.11
C


ATOM
9641
NH1
ARG
B
71
74.350
25.911
−4.514
1.00
25.15
N


ATOM
9644
NH2
ARG
B
71
73.264
24.521
−5.956
1.00
26.65
N


ATOM
9647
C
ARG
B
71
71.898
31.131
−5.891
1.00
18.75
C


ATOM
9648
O
ARG
B
71
72.317
31.075
−4.730
1.00
18.06
O


ATOM
9650
N
PHE
B
72
71.805
32.273
−6.578
1.00
19.13
N


ATOM
9651
CA
PHE
B
72
72.245
33.561
−6.035
1.00
18.99
C


ATOM
9653
CB
PHE
B
72
73.466
34.067
−6.802
1.00
19.20
C


ATOM
9656
CG
PHE
B
72
74.665
33.186
−6.678
1.00
17.20
C


ATOM
9657
CD1
PHE
B
72
75.477
33.264
−5.567
1.00
13.20
C


ATOM
9659
CE1
PHE
B
72
76.592
32.453
−5.452
1.00
17.06
C


ATOM
9661
CZ
PHE
B
72
76.903
31.559
−6.461
1.00
16.63
C


ATOM
9663
CE2
PHE
B
72
76.103
31.483
−7.575
1.00
16.38
C


ATOM
9665
CD2
PHE
B
72
74.991
32.293
−7.682
1.00
16.36
C


ATOM
9667
C
PHE
B
72
71.156
34.618
−6.123
1.00
19.06
C


ATOM
9668
O
PHE
B
72
71.445
35.800
−6.280
1.00
17.60
O


ATOM
9670
N
GLU
B
73
69.904
34.204
−5.995
1.00
20.54
N


ATOM
9671
CA
GLU
B
73
68.804
35.097
−6.302
1.00
22.01
C


ATOM
9673
CB
GLU
B
73
67.486
34.340
−6.386
1.00
22.80
C


ATOM
9676
CG
GLU
B
73
66.339
35.221
−6.820
1.00
28.79
C


ATOM
9679
CD
GLU
B
73
65.066
34.454
−7.040
1.00
35.71
C


ATOM
9680
OE1
GLU
B
73
65.099
33.444
−7.775
1.00
41.78
O


ATOM
9681
OE2
GLU
B
73
64.029
34.877
−6.491
1.00
40.65
O


ATOM
9682
C
GLU
B
73
68.688
36.247
−5.314
1.00
22.08
C


ATOM
9683
O
GLU
B
73
68.551
37.400
−5.730
1.00
22.85
O


ATOM
9685
N
SER
B
74
68.731
35.953
−4.017
1.00
21.68
N


ATOM
9686
CA
SER
B
74
68.605
37.021
−3.013
1.00
21.91
C


ATOM
9688
CB
SER
B
74
68.324
36.455
−1.623
1.00
21.64
C


ATOM
9691
OG
SER
B
74
69.005
35.236
−1.449
1.00
28.40
O


ATOM
9693
C
SER
B
74
69.839
37.924
−3.003
1.00
20.34
C


ATOM
9694
O
SER
B
74
69.740
39.116
−2.725
1.00
20.35
O


ATOM
9696
N
ASP
B
75
70.995
37.353
−3.317
1.00
19.90
N


ATOM
9697
CA
ASP
B
75
72.215
38.134
−3.510
1.00
18.61
C


ATOM
9699
CB
ASP
B
75
73.408
37.202
−3.761
1.00
18.73
C


ATOM
9702
CG
ASP
B
75
73.823
36.415
−2.516
1.00
20.70
C


ATOM
9703
OD1
ASP
B
75
73.848
37.000
−1.410
1.00
20.76
O


ATOM
9704
OD2
ASP
B
75
74.141
35.210
−2.647
1.00
25.11
O


ATOM
9705
C
ASP
B
75
72.050
39.108
−4.679
1.00
17.25
C


ATOM
9706
O
ASP
B
75
72.412
40.278
−4.586
1.00
15.83
O


ATOM
9708
N
ILE
B
76
71.483
38.621
−5.777
1.00
17.11
N


ATOM
9709
CA
ILE
B
76
71.293
39.443
−6.966
1.00
16.05
C


ATOM
9711
CB
ILE
B
76
70.902
38.583
−8.195
1.00
16.10
C


ATOM
9713
CG1
ILE
B
76
72.111
37.768
−8.665
1.00
15.10
C


ATOM
9716
CD1
ILE
B
76
71.744
36.506
−9.406
1.00
11.65
C


ATOM
9720
CG2
ILE
B
76
70.375
39.455
−9.344
1.00
12.09
C


ATOM
9724
C
ILE
B
76
70.265
40.543
−6.710
1.00
16.87
C


ATOM
9725
O
ILE
B
76
70.449
41.672
−7.163
1.00
18.28
O


ATOM
9727
N
ARG
B
77
69.198
40.231
−5.980
1.00
16.96
N


ATOM
9728
CA
ARG
B
77
68.194
41.248
−5.651
1.00
18.72
C


ATOM
9730
CB
ARG
B
77
67.021
40.645
−4.874
1.00
20.75
C


ATOM
9733
CG
ARG
B
77
66.166
39.656
−5.661
1.00
29.90
C


ATOM
9736
CD
ARG
B
77
65.265
38.856
−4.720
1.00
40.32
C


ATOM
9739
NE
ARG
B
77
64.403
39.739
−3.935
1.00
48.74
N


ATOM
9741
CZ
ARG
B
77
63.323
40.363
−4.409
1.00
58.45
C


ATOM
9742
NH1
ARG
B
77
62.941
40.209
−5.677
1.00
61.84
N


ATOM
9745
NH2
ARG
B
77
62.612
41.149
−3.608
1.00
61.27
N


ATOM
9748
C
ARG
B
77
68.824
42.359
−4.820
1.00
17.11
C


ATOM
9749
O
ARG
B
77
68.705
43.539
−5.144
1.00
16.80
O


ATOM
9751
N
GLY
B
78
69.507
41.962
−3.752
1.00
15.79
N


ATOM
9752
CA
GLY
B
78
70.179
42.899
−2.865
1.00
15.46
C


ATOM
9755
C
GLY
B
78
71.246
43.712
−3.564
1.00
15.64
C


ATOM
9756
O
GLY
B
78
71.489
44.862
−3.199
1.00
17.03
O


ATOM
9758
N
ALA
B
79
71.885
43.119
−4.569
1.00
15.27
N


ATOM
9759
CA
ALA
B
79
72.904
43.817
−5.344
1.00
15.39
C


ATOM
9761
CB
ALA
B
79
73.746
42.825
−6.147
1.00
14.40
C


ATOM
9765
C
ALA
B
79
72.242
44.845
−6.261
1.00
15.93
C


ATOM
9766
O
ALA
B
79
72.778
45.936
−6.470
1.00
15.93
O


ATOM
9768
N
LEU
B
80
71.074
44.492
−6.790
1.00
16.63
N


ATOM
9769
CA
LEU
B
80
70.282
45.416
−7.599
1.00
18.25
C


ATOM
9771
CB
LEU
B
80
69.142
44.682
−8.318
1.00
18.14
C


ATOM
9774
CG
LEU
B
80
69.583
43.825
−9.500
1.00
17.03
C


ATOM
9776
CD1
LEU
B
80
68.451
42.934
−9.958
1.00
16.74
C


ATOM
9780
CD2
LEU
B
80
70.083
44.711
−10.629
1.00
12.66
C


ATOM
9784
C
LEU
B
80
69.716
46.539
−6.746
1.00
19.03
C


ATOM
9785
O
LEU
B
80
69.682
47.693
−7.175
1.00
18.91
O


ATOM
9787
N
ASP
B
81
69.276
46.203
−5.537
1.00
20.51
N


ATOM
9788
CA
ASP
B
81
68.782
47.216
−4.617
1.00
21.82
C


ATOM
9790
CB
ASP
B
81
68.362
46.599
−3.283
1.00
22.67
C


ATOM
9793
CG
ASP
B
81
67.442
47.512
−2.487
1.00
28.11
C


ATOM
9794
OD1
ASP
B
81
66.340
47.835
−2.986
1.00
34.60
O


ATOM
9795
OD2
ASP
B
81
67.814
47.902
−1.360
1.00
34.51
O


ATOM
9796
C
ASP
B
81
69.855
48.271
−4.398
1.00
21.60
C


ATOM
9797
O
ASP
B
81
69.604
49.460
−4.567
1.00
22.28
O


ATOM
9799
N
AARG
B
82
71.048
47.810
−4.034
0.50
21.77
N


ATOM
9800
N
BARG
B
82
71.060
47.835
−4.037
0.50
21.42
N


ATOM
9801
CA
AARG
B
82
72.210
48.668
−3.815
0.50
21.62
C


ATOM
9802
CA
BARG
B
82
72.170
48.761
−3.802
0.50
20.97
C


ATOM
9805
CB
AARG
B
82
73.395
47.796
−3.398
0.50
21.78
C


ATOM
9806
CB
BARG
B
82
73.377
48.032
−3.207
0.50
20.77
C


ATOM
9811
CG
AARG
B
82
74.632
48.551
−2.977
0.50
21.50
C


ATOM
9812
CG
BARG
B
82
74.483
48.970
−2.749
0.50
19.14
C


ATOM
9817
CD
AARG
B
82
75.727
47.582
−2.543
0.50
22.44
C


ATOM
9818
CD
BARG
B
82
75.729
48.220
−2.295
0.50
17.73
C


ATOM
9823
NE
AARG
B
82
75.222
46.533
−1.655
0.50
22.33
N


ATOM
9824
NE
BARG
B
82
75.472
47.340
−1.155
0.50
15.30
N


ATOM
9827
CZ
AARG
B
82
75.137
45.245
−1.979
0.50
18.58
C


ATOM
9828
CZ
BARG
B
82
75.716
47.658
0.112
0.50
9.61
C


ATOM
9829
NH1
AARG
B
82
74.666
44.374
−1.100
0.50
19.31
N


ATOM
9830
NH1
BARG
B
82
75.453
46.790
1.074
0.50
10.27
N


ATOM
9835
NH2
AARG
B
82
75.532
44.826
−3.173
0.50
16.77
N


ATOM
9836
NH2
BARG
B
82
76.225
48.838
0.419
0.50
10.60
N


ATOM
9841
C
AARG
B
82
72.572
49.461
−5.069
0.50
21.39
C


ATOM
9842
C
BARG
B
82
72.583
49.484
−5.082
0.50
21.04
C


ATOM
9843
O
AARG
B
82
72.945
50.628
−4.987
0.50
21.43
O


ATOM
9844
O
BARG
B
82
73.011
50.634
−5.030
0.50
21.11
O


ATOM
9847
N
PHE
B
83
72.461
48.810
−6.225
1.00
21.32
N


ATOM
9848
CA
PHE
B
83
72.709
49.442
−7.524
1.00
20.89
C


ATOM
9850
CB
PHE
B
83
72.515
48.422
−8.655
1.00
20.59
C


ATOM
9853
CG
PHE
B
83
72.552
49.020
−10.035
1.00
20.93
C


ATOM
9854
CD1
PHE
B
83
73.763
49.277
−10.664
1.00
20.95
C


ATOM
9856
CE1
PHE
B
83
73.800
49.831
−11.936
1.00
20.41
C


ATOM
9858
CZ
PHE
B
83
72.621
50.133
−12.595
1.00
21.33
C


ATOM
9860
CE2
PHE
B
83
71.407
49.879
−11.982
1.00
22.56
C


ATOM
9862
CD2
PHE
B
83
71.375
49.324
−10.709
1.00
22.55
C


ATOM
9864
C
PHE
B
83
71.797
50.654
−7.741
1.00
21.23
C


ATOM
9865
O
PHE
B
83
72.244
51.683
−8.243
1.00
21.52
O


ATOM
9867
N
VAL
B
84
70.531
50.532
−7.346
1.00
21.76
N


ATOM
9868
CA
VAL
B
84
69.567
51.624
−7.497
1.00
22.25
C


ATOM
9870
CB
VAL
B
84
68.105
51.130
−7.366
1.00
22.54
C


ATOM
9872
CG1
VAL
B
84
67.127
52.289
−7.571
1.00
19.41
C


ATOM
9876
CG2
VAL
B
84
67.823
50.009
−8.361
1.00
19.62
C


ATOM
9880
C
VAL
B
84
69.804
52.726
−6.464
1.00
23.27
C


ATOM
9881
O
VAL
B
84
69.968
53.891
−6.826
1.00
23.34
O


ATOM
9883
N
SER
B
85
69.837
52.347
−5.187
1.00
24.43
N


ATOM
9884
CA
SER
B
85
69.931
53.311
−4.079
1.00
25.59
C


ATOM
9886
CB
SER
B
85
69.773
52.598
−2.730
1.00
25.76
C


ATOM
9889
OG
SER
B
85
70.966
51.936
−2.345
1.00
28.79
O


ATOM
9891
C
SER
B
85
71.222
54.147
−4.070
1.00
25.80
C


ATOM
9892
O
SER
B
85
71.237
55.262
−3.539
1.00
25.61
O


ATOM
9894
N
SER
B
86
72.294
53.608
−4.647
1.00
25.84
N


ATOM
9895
CA
SER
B
86
73.557
54.329
−4.745
1.00
26.12
C


ATOM
9897
CB
SER
B
86
74.736
53.354
−4.674
1.00
26.10
C


ATOM
9900
OG
SER
B
86
74.773
52.496
−5.799
1.00
25.33
O


ATOM
9902
C
SER
B
86
73.630
55.169
−6.026
1.00
27.01
C


ATOM
9903
O
SER
B
86
74.671
55.754
−6.332
1.00
26.74
O


ATOM
9905
N
GLY
B
87
72.526
55.223
−6.771
1.00
27.80
N


ATOM
9906
CA
GLY
B
87
72.423
56.076
−7.955
1.00
27.61
C


ATOM
9909
C
GLY
B
87
72.990
55.452
−9.220
1.00
27.86
C


ATOM
9910
O
GLY
B
87
73.196
56.144
−10.221
1.00
28.30
O


ATOM
9912
N
GLY
B
88
73.243
54.147
−9.190
1.00
27.52
N


ATOM
9913
CA
GLY
B
88
73.755
53.453
−10.367
1.00
27.36
C


ATOM
9916
C
GLY
B
88
72.811
53.557
−11.554
1.00
27.07
C


ATOM
9917
O
GLY
B
88
73.249
53.733
−12.694
1.00
27.18
O


ATOM
9919
N
PHE
B
89
71.512
53.463
−11.289
1.00
26.60
N


ATOM
9920
CA
PHE
B
89
70.525
53.475
−12.362
1.00
26.75
C


ATOM
9922
CB
PHE
B
89
69.150
53.030
−11.846
1.00
26.20
C


ATOM
9925
CG
PHE
B
89
68.167
52.700
−12.935
1.00
24.08
C


ATOM
9926
CD1
PHE
B
89
68.462
51.735
−13.891
1.00
24.55
C


ATOM
9928
CE1
PHE
B
89
67.552
51.427
−14.907
1.00
22.92
C


ATOM
9930
CZ
PHE
B
89
66.336
52.082
−14.965
1.00
23.72
C


ATOM
9932
CE2
PHE
B
89
66.026
53.044
−14.010
1.00
25.36
C


ATOM
9934
CD2
PHE
B
89
66.939
53.344
−13.000
1.00
24.97
C


ATOM
9936
C
PHE
B
89
70.464
54.862
−12.992
1.00
28.10
C


ATOM
9937
O
PHE
B
89
70.479
54.990
−14.219
1.00
28.61
O


ATOM
9939
N
ASP
B
90
70.428
55.895
−12.150
1.00
28.87
N


ATOM
9940
CA
ASP
B
90
70.424
57.283
−12.619
1.00
29.31
C


ATOM
9942
CB
ASP
B
90
70.369
58.267
−11.435
1.00
30.20
C


ATOM
9945
CG
ASP
B
90
69.689
59.592
−11.796
1.00
35.08
C


ATOM
9946
OD1
ASP
B
90
68.489
59.562
−12.148
1.00
41.57
O


ATOM
9947
OD2
ASP
B
90
70.343
60.661
−11.713
1.00
36.53
O


ATOM
9948
C
ASP
B
90
71.666
57.558
−13.467
1.00
28.83
C


ATOM
9949
O
ASP
B
90
71.590
58.238
−14.492
1.00
28.69
O


ATOM
9951
N
ALA
B
91
72.804
57.013
−13.040
1.00
28.69
N


ATOM
9952
CA
ALA
B
91
74.079
57.231
−13.729
1.00
28.58
C


ATOM
9954
CB
ALA
B
91
75.238
56.654
−12.906
1.00
28.14
C


ATOM
9958
C
ALA
B
91
74.084
56.636
−15.137
1.00
28.25
C


ATOM
9959
O
ALA
B
91
74.486
57.300
−16.092
1.00
28.14
O


ATOM
9961
N
VAL
B
92
73.637
55.388
−15.261
1.00
27.93
N


ATOM
9962
CA
VAL
B
92
73.674
54.696
−16.550
1.00
27.65
C


ATOM
9964
CB
VAL
B
92
73.433
53.164
−16.416
1.00
27.48
C


ATOM
9966
CG1
VAL
B
92
74.505
52.532
−15.551
1.00
23.58
C


ATOM
9970
CG2
VAL
B
92
72.053
52.869
−15.861
1.00
28.01
C


ATOM
9974
C
VAL
B
92
72.685
55.313
−17.536
1.00
28.14
C


ATOM
9975
O
VAL
B
92
72.979
55.407
−18.728
1.00
28.47
O


ATOM
9977
N
THR
B
93
71.529
55.753
−17.039
1.00
28.50
N


ATOM
9978
CA
THR
B
93
70.538
56.414
−17.892
1.00
28.67
C


ATOM
9980
CB
THR
B
93
69.208
56.708
−17.154
1.00
28.40
C


ATOM
9982
OG1
THR
B
93
69.425
57.657
−16.104
1.00
29.60
O


ATOM
9984
CG2
THR
B
93
68.612
55.436
−16.579
1.00
28.96
C


ATOM
9988
C
THR
B
93
71.079
57.715
−18.481
1.00
28.92
C


ATOM
9989
O
THR
B
93
70.649
58.128
−19.553
1.00
29.54
O


ATOM
9991
N
LYS
B
94
72.019
58.355
−17.789
1.00
29.23
N


ATOM
9992
CA
LYS
B
94
72.629
59.587
−18.297
1.00
30.17
C


ATOM
9994
CB
LYS
B
94
72.918
60.566
−17.141
1.00
30.56
C


ATOM
9997
CG
LYS
B
94
71.692
61.007
−16.331
1.00
34.56
C


ATOM
10000
CD
LYS
B
94
70.653
61.747
−17.185
1.00
41.93
C


ATOM
10003
CE
LYS
B
94
69.275
61.775
−16.518
1.00
46.78
C


ATOM
10006
NZ
LYS
B
94
68.163
61.856
−17.518
1.00
45.92
N


ATOM
10010
C
LYS
B
94
73.908
59.367
−19.125
1.00
29.35
C


ATOM
10011
O
LYS
B
94
74.482
60.339
−19.615
1.00
30.59
O


ATOM
10013
N
THR
B
95
74.345
58.119
−19.305
1.00
28.07
N


ATOM
10014
CA
THR
B
95
75.658
57.854
−19.922
1.00
26.85
C


ATOM
10016
CB
THR
B
95
76.745
57.665
−18.839
1.00
26.84
C


ATOM
10018
OG1
THR
B
95
76.483
56.470
−18.092
1.00
26.04
O


ATOM
10020
CG2
THR
B
95
76.788
58.861
−17.888
1.00
26.33
C


ATOM
10024
C
THR
B
95
75.765
56.645
−20.861
1.00
26.37
C


ATOM
10025
O
THR
B
95
76.606
56.656
−21.760
1.00
26.38
O


ATOM
10027
N
SER
B
96
74.948
55.610
−20.655
1.00
25.35
N


ATOM
10028
CA
SER
B
96
75.172
54.314
−21.308
1.00
24.42
C


ATOM
10030
CB
SER
B
96
76.055
53.449
−20.409
1.00
24.35
C


ATOM
10033
OG
SER
B
96
76.202
52.142
−20.932
1.00
24.77
O


ATOM
10035
C
SER
B
96
73.892
53.540
−21.631
1.00
24.01
C


ATOM
10036
O
SER
B
96
73.109
53.211
−20.740
1.00
23.55
O


ATOM
10038
N
LEU
B
97
73.702
53.229
−22.910
1.00
23.48
N


ATOM
10039
CA
LEU
B
97
72.568
52.423
−23.351
1.00
22.24
C


ATOM
10041
CB
LEU
B
97
72.443
52.453
−24.877
1.00
22.40
C


ATOM
10044
CG
LEU
B
97
71.212
51.750
−25.464
1.00
22.64
C


ATOM
10046
CD1
LEU
B
97
69.935
52.503
−25.117
1.00
19.62
C


ATOM
10050
CD2
LEU
B
97
71.346
51.603
−26.969
1.00
23.82
C


ATOM
10054
C
LEU
B
97
72.727
50.988
−22.883
1.00
20.77
C


ATOM
10055
O
LEU
B
97
71.755
50.352
−22.492
1.00
20.47
O


ATOM
10057
N
HIS
B
98
73.955
50.480
−22.943
1.00
20.09
N


ATOM
10058
CA
HIS
B
98
74.252
49.112
−22.517
1.00
19.77
C


ATOM
10060
CB
HIS
B
98
75.736
48.786
−22.738
1.00
19.72
C


ATOM
10063
CG
HIS
B
98
76.151
47.444
−22.215
1.00
22.81
C


ATOM
10064
ND1
HIS
B
98
75.554
46.269
−22.619
1.00
23.34
N


ATOM
10066
CE1
HIS
B
98
76.118
45.251
−21.993
1.00
22.61
C


ATOM
10068
NE2
HIS
B
98
77.064
45.722
−21.201
1.00
27.27
N


ATOM
10070
CD2
HIS
B
98
77.108
47.092
−21.322
1.00
25.81
C


ATOM
10072
C
HIS
B
98
73.862
48.928
−21.052
1.00
18.84
C


ATOM
10073
O
HIS
B
98
73.128
47.998
−20.709
1.00
17.29
O


ATOM
10075
N
GLY
B
99
74.340
49.833
−20.202
1.00
18.20
N


ATOM
10076
CA
GLY
B
99
74.023
49.803
−18.779
1.00
17.26
C


ATOM
10079
C
GLY
B
99
72.536
49.941
−18.538
1.00
16.93
C


ATOM
10080
O
GLY
B
99
71.958
49.206
−17.741
1.00
17.18
O


ATOM
10082
N
THR
B
100
71.914
50.875
−19.246
1.00
16.49
N


ATOM
10083
CA
THR
B
100
70.496
51.144
−19.079
1.00
16.09
C


ATOM
10085
CB
THR
B
100
70.067
52.381
−19.885
1.00
16.31
C


ATOM
10087
OG1
THR
B
100
70.820
53.517
−19.442
1.00
15.45
O


ATOM
10089
CG2
THR
B
100
68.579
52.662
−19.706
1.00
13.65
C


ATOM
10093
C
THR
B
100
69.663
49.944
−19.502
1.00
16.21
C


ATOM
10094
O
THR
B
100
68.809
49.484
−18.749
1.00
16.53
O


ATOM
10096
N
ALA
B
101
69.925
49.432
−20.699
1.00
16.10
N


ATOM
10097
CA
ALA
B
101
69.173
48.295
−21.228
1.00
15.62
C


ATOM
10099
CB
ALA
B
101
69.590
48.005
−22.656
1.00
14.94
C


ATOM
10103
C
ALA
B
101
69.344
47.050
−20.355
1.00
15.19
C


ATOM
10104
O
ALA
B
101
68.377
46.331
−20.088
1.00
15.37
O


ATOM
10106
N
LEU
B
102
70.570
46.808
−19.906
1.00
14.63
N


ATOM
10107
CA
LEU
B
102
70.870
45.637
−19.087
1.00
14.71
C


ATOM
10109
CB
LEU
B
102
72.386
45.406
−18.992
1.00
13.92
C


ATOM
10112
CG
LEU
B
102
72.865
44.181
−18.209
1.00
13.02
C


ATOM
10114
CD1
LEU
B
102
72.131
42.914
−18.635
1.00
10.45
C


ATOM
10118
CD2
LEU
B
102
74.360
43.996
−18.367
1.00
7.35
C


ATOM
10122
C
LEU
B
102
70.268
45.775
−17.697
1.00
15.21
C


ATOM
10123
O
LEU
B
102
69.675
44.826
−17.185
1.00
15.85
O


ATOM
10125
N
SER
B
103
70.412
46.950
−17.087
1.00
15.38
N


ATOM
10126
CA
SER
B
103
69.852
47.177
−15.756
1.00
15.40
C


ATOM
10128
CB
SER
B
103
70.368
48.483
−15.141
1.00
16.04
C


ATOM
10131
OG
SER
B
103
70.205
49.589
−16.011
1.00
17.82
O


ATOM
10133
C
SER
B
103
68.329
47.171
−15.816
1.00
15.54
C


ATOM
10134
O
SER
B
103
67.670
46.623
−14.928
1.00
15.63
O


ATOM
10136
N
PHE
B
104
67.771
47.759
−16.871
1.00
15.00
N


ATOM
10137
CA
PHE
B
104
66.331
47.747
−17.056
1.00
15.05
C


ATOM
10139
CB
PHE
B
104
65.933
48.364
−18.399
1.00
15.62
C


ATOM
10142
CG
PHE
B
104
64.445
48.436
−18.610
1.00
15.05
C


ATOM
10143
CD1
PHE
B
104
63.751
47.357
−19.128
1.00
16.50
C


ATOM
10145
CE1
PHE
B
104
62.376
47.425
−19.324
1.00
19.68
C


ATOM
10147
CZ
PHE
B
104
61.686
48.577
−18.995
1.00
16.19
C


ATOM
10149
CE2
PHE
B
104
62.368
49.657
−18.473
1.00
13.76
C


ATOM
10151
CD2
PHE
B
104
63.738
49.584
−18.283
1.00
15.00
C


ATOM
10153
C
PHE
B
104
65.834
46.319
−16.983
1.00
15.00
C


ATOM
10154
O
PHE
B
104
64.974
45.998
−16.158
1.00
16.23
O


ATOM
10156
N
ARG
B
105
66.395
45.463
−17.831
1.00
14.08
N


ATOM
10157
CA
ARG
B
105
65.986
44.063
−17.889
1.00
14.06
C


ATOM
10159
CB
ARG
B
105
66.743
43.309
−18.988
1.00
14.27
C


ATOM
10162
CG
ARG
B
105
66.461
41.808
−19.011
1.00
13.50
C


ATOM
10165
CD
ARG
B
105
66.936
41.164
−20.294
1.00
12.74
C


ATOM
10168
NE
ARG
B
105
68.391
41.180
−20.429
1.00
14.09
N


ATOM
10170
CZ
ARG
B
105
69.232
40.315
−19.865
1.00
11.59
C


ATOM
10171
NH1
ARG
B
105
68.796
39.340
−19.083
1.00
16.77
N


ATOM
10174
NH2
ARG
B
105
70.532
40.436
−20.078
1.00
15.03
N


ATOM
10177
C
ARG
B
105
66.162
43.345
−16.551
1.00
13.99
C


ATOM
10178
O
ARG
B
105
65.239
42.682
−16.089
1.00
14.90
O


ATOM
10180
N
LEU
B
106
67.332
43.468
−15.929
1.00
13.46
N


ATOM
10181
CA
LEU
B
106
67.584
42.741
−14.680
1.00
13.96
C


ATOM
10183
CB
LEU
B
106
69.051
42.839
−14.254
1.00
13.53
C


ATOM
10186
CG
LEU
B
106
70.091
42.293
−15.239
1.00
12.65
C


ATOM
10188
CD1
LEU
B
106
71.503
42.626
−14.741
1.00
9.23
C


ATOM
10192
CD2
LEU
B
106
69.921
40.809
−15.472
1.00
6.67
C


ATOM
10196
C
LEU
B
106
66.677
43.241
−13.552
1.00
14.63
C


ATOM
10197
O
LEU
B
106
66.173
42.448
−12.762
1.00
15.27
O


ATOM
10199
N
LEU
B
107
66.458
44.551
−13.491
1.00
14.67
N


ATOM
10200
CA
LEU
B
107
65.586
45.137
−12.477
1.00
14.25
C


ATOM
10202
CB
LEU
B
107
65.628
46.660
−12.564
1.00
14.40
C


ATOM
10205
CG
LEU
B
107
66.887
47.308
−11.999
1.00
12.30
C


ATOM
10207
CD1
LEU
B
107
67.049
48.692
−12.571
1.00
7.43
C


ATOM
10211
CD2
LEU
B
107
66.824
47.340
−10.475
1.00
7.16
C


ATOM
10215
C
LEU
B
107
64.142
44.652
−12.612
1.00
14.77
C


ATOM
10216
O
LEU
B
107
63.538
44.206
−11.630
1.00
14.29
O


ATOM
10218
N
ARG
B
108
63.592
44.737
−13.821
1.00
15.25
N


ATOM
10219
CA
ARG
B
108
62.222
44.261
−14.060
1.00
15.49
C


ATOM
10221
CB
ARG
B
108
61.740
44.576
−15.482
1.00
14.33
C


ATOM
10224
CG
ARG
B
108
60.359
44.011
−15.784
1.00
13.69
C


ATOM
10227
CD
ARG
B
108
59.670
44.725
−16.944
1.00
14.55
C


ATOM
10230
NE
ARG
B
108
59.210
46.070
−16.585
1.00
14.46
N


ATOM
10232
CZ
ARG
B
108
58.646
46.926
−17.437
1.00
11.54
C


ATOM
10233
NH1
ARG
B
108
58.454
46.593
−18.704
1.00
14.47
N


ATOM
10236
NH2
ARG
B
108
58.273
48.125
−17.022
1.00
12.97
N


ATOM
10239
C
ARG
B
108
62.116
42.765
−13.796
1.00
15.68
C


ATOM
10240
O
ARG
B
108
61.127
42.298
−13.208
1.00
15.93
O


ATOM
10242
N
GLN
B
109
63.134
42.023
−14.228
1.00
15.71
N


ATOM
10243
CA
GLN
B
109
63.177
40.579
−14.012
1.00
16.54
C


ATOM
10245
CB
GLN
B
109
64.498
39.986
−14.522
1.00
16.76
C


ATOM
10248
CG
GLN
B
109
64.609
38.459
−14.387
1.00
17.37
C


ATOM
10251
CD
GLN
B
109
66.016
37.939
−14.642
1.00
17.59
C


ATOM
10252
OE1
GLN
B
109
66.864
38.634
−15.208
1.00
18.95
O


ATOM
10253
NE2
GLN
B
109
66.268
36.709
−14.221
1.00
15.92
N


ATOM
10256
C
GLN
B
109
63.008
40.254
−12.536
1.00
17.26
C


ATOM
10257
O
GLN
B
109
62.312
39.311
−12.184
1.00
19.23
O


ATOM
10259
N
HIS
B
110
63.639
41.040
−11.674
1.00
17.44
N


ATOM
10260
CA
HIS
B
110
63.621
40.755
−10.241
1.00
18.03
C


ATOM
10262
CB
HIS
B
110
65.033
40.933
−9.667
1.00
18.14
C


ATOM
10265
CG
HIS
B
110
65.980
39.860
−10.110
1.00
18.35
C


ATOM
10266
ND1
HIS
B
110
66.176
38.701
−9.390
1.00
19.17
N


ATOM
10268
CE1
HIS
B
110
67.037
37.930
−10.028
1.00
19.08
C


ATOM
10270
NE2
HIS
B
110
67.396
38.540
−11.144
1.00
15.83
N


ATOM
10272
CD2
HIS
B
110
66.744
39.746
−11.222
1.00
15.87
C


ATOM
10274
C
HIS
B
110
62.561
41.554
−9.473
1.00
18.48
C


ATOM
10275
O
HIS
B
110
62.619
41.669
−8.246
1.00
18.21
O


ATOM
10277
N
GLY
B
111
61.588
42.093
−10.207
1.00
18.75
N


ATOM
10278
CA
GLY
B
111
60.413
42.721
−9.613
1.00
18.98
C


ATOM
10281
C
GLY
B
111
60.509
44.196
−9.265
1.00
19.81
C


ATOM
10282
O
GLY
B
111
59.596
44.726
−8.635
1.00
20.39
O


ATOM
10284
N
PHE
B
112
61.597
44.864
−9.655
1.00
20.29
N


ATOM
10285
CA
PHE
B
112
61.740
46.301
−9.398
1.00
20.92
C


ATOM
10287
CB
PHE
B
112
63.200
46.746
−9.510
1.00
20.72
C


ATOM
10290
CG
PHE
B
112
64.079
46.239
−8.400
1.00
23.40
C


ATOM
10291
CD1
PHE
B
112
64.259
46.986
−7.241
1.00
24.03
C


ATOM
10293
CE1
PHE
B
112
65.071
46.521
−6.217
1.00
25.01
C


ATOM
10295
CZ
PHE
B
112
65.718
45.299
−6.348
1.00
24.81
C


ATOM
10297
CE2
PHE
B
112
65.551
44.550
−7.499
1.00
22.54
C


ATOM
10299
CD2
PHE
B
112
64.737
45.018
−8.516
1.00
25.22
C


ATOM
10301
C
PHE
B
112
60.892
47.101
−10.376
1.00
21.54
C


ATOM
10302
O
PHE
B
112
60.670
46.671
−11.505
1.00
22.97
O


ATOM
10304
N
GLU
B
113
60.417
48.261
−9.937
1.00
21.84
N


ATOM
10305
CA
GLU
B
113
59.687
49.176
−10.811
1.00
22.54
C


ATOM
10307
CB
GLU
B
113
58.781
50.129
−10.015
1.00
23.84
C


ATOM
10310
CG
GLU
B
113
57.290
49.807
−10.092
1.00
31.72
C


ATOM
10313
CD
GLU
B
113
56.401
51.051
−9.956
1.00
38.67
C


ATOM
10314
OE1
GLU
B
113
56.886
52.091
−9.453
1.00
35.63
O


ATOM
10315
OE2
GLU
B
113
55.213
50.983
−10.355
1.00
43.29
O


ATOM
10316
C
GLU
B
113
60.655
50.009
−11.651
1.00
20.65
C


ATOM
10317
O
GLU
B
113
61.524
50.701
−11.117
1.00
18.56
O


ATOM
10319
N
VAL
B
114
60.480
49.938
−12.965
1.00
19.18
N


ATOM
10320
CA
VAL
B
114
61.234
50.749
−13.903
1.00
17.42
C


ATOM
10322
CB
VAL
B
114
62.468
50.005
−14.455
1.00
17.60
C


ATOM
10324
CG1
VAL
B
114
63.606
50.049
−13.453
1.00
17.18
C


ATOM
10328
CG2
VAL
B
114
62.119
48.567
−14.827
1.00
14.25
C


ATOM
10332
C
VAL
B
114
60.326
51.133
−15.056
1.00
17.15
C


ATOM
10333
O
VAL
B
114
59.516
50.329
−15.519
1.00
16.77
O


ATOM
10335
N
SER
B
115
60.476
52.370
−15.509
1.00
17.50
N


ATOM
10336
CA
SER
B
115
59.654
52.937
−16.560
1.00
17.82
C


ATOM
10338
CB
SER
B
115
59.297
54.381
−16.201
1.00
18.13
C


ATOM
10341
OG
SER
B
115
58.501
54.983
−17.208
1.00
21.17
O


ATOM
10343
C
SER
B
115
60.410
52.919
−17.878
1.00
17.73
C


ATOM
10344
O
SER
B
115
61.635
52.960
−17.899
1.00
17.41
O


ATOM
10346
N
GLN
B
116
59.674
52.876
−18.984
1.00
18.27
N


ATOM
10347
CA
GLN
B
116
60.288
52.972
−20.304
1.00
17.86
C


ATOM
10349
CB
GLN
B
116
59.264
52.661
−21.388
1.00
17.57
C


ATOM
10352
CG
GLN
B
116
58.150
53.677
−21.508
1.00
17.76
C


ATOM
10355
CD
GLN
B
116
57.264
53.437
−22.707
1.00
17.92
C


ATOM
10356
OE1
GLN
B
116
57.410
52.443
−23.423
1.00
16.41
O


ATOM
10357
NE2
GLN
B
116
56.334
54.354
−22.937
1.00
17.49
N


ATOM
10360
C
GLN
B
116
60.917
54.345
−20.564
1.00
18.48
C


ATOM
10361
O
GLN
B
116
61.710
54.496
−21.490
1.00
18.49
O


ATOM
10363
N
GLU
B
117
60.561
55.340
−19.751
1.00
19.64
N


ATOM
10364
CA
GLU
B
117
61.135
56.688
−19.869
1.00
20.82
C


ATOM
10366
CB
GLU
B
117
60.381
57.694
−18.986
1.00
21.40
C


ATOM
10369
CG
GLU
B
117
58.875
57.811
−19.258
1.00
26.23
C


ATOM
10372
CD
GLU
B
117
58.536
58.649
−20.486
1.00
33.68
C


ATOM
10373
OE1
GLU
B
117
59.219
58.514
−21.526
1.00
37.95
O


ATOM
10374
OE2
GLU
B
117
57.568
59.441
−20.414
1.00
38.43
O


ATOM
10375
C
GLU
B
117
62.617
56.692
−19.500
1.00
20.57
C


ATOM
10376
O
GLU
B
117
63.321
57.674
−19.746
1.00
21.48
O


ATOM
10378
N
ALA
B
118
63.080
55.597
−18.903
1.00
20.14
N


ATOM
10379
CA
ALA
B
118
64.498
55.381
−18.653
1.00
20.12
C


ATOM
10381
CB
ALA
B
118
64.703
54.037
−17.973
1.00
19.71
C


ATOM
10385
C
ALA
B
118
65.356
55.463
−19.921
1.00
20.46
C


ATOM
10386
O
ALA
B
118
66.550
55.721
−19.829
1.00
21.08
O


ATOM
10388
N
PHE
B
119
64.757
55.235
−21.092
1.00
20.70
N


ATOM
10389
CA
PHE
B
119
65.491
55.273
−22.367
1.00
21.14
C


ATOM
10391
CB
PHE
B
119
64.970
54.171
−23.298
1.00
20.40
C


ATOM
10394
CG
PHE
B
119
65.206
52.777
−22.788
1.00
19.10
C


ATOM
10395
CD1
PHE
B
119
66.462
52.198
−22.868
1.00
17.86
C


ATOM
10397
CE1
PHE
B
119
66.679
50.899
−22.401
1.00
17.10
C


ATOM
10399
CZ
PHE
B
119
65.630
50.170
−21.862
1.00
17.94
C


ATOM
10401
CE2
PHE
B
119
64.374
50.733
−21.782
1.00
17.83
C


ATOM
10403
CD2
PHE
B
119
64.163
52.031
−22.244
1.00
20.16
C


ATOM
10405
C
PHE
B
119
65.411
56.626
−23.095
1.00
22.24
C


ATOM
10406
O
PHE
B
119
65.893
56.758
−24.223
1.00
22.20
O


ATOM
10408
N
SER
B
120
64.812
57.625
−22.451
1.00
24.13
N


ATOM
10409
CA
SER
B
120
64.542
58.925
−23.087
1.00
25.19
C


ATOM
10411
CB
SER
B
120
63.829
59.853
−22.105
1.00
25.36
C


ATOM
10414
OG
SER
B
120
64.675
60.154
−21.007
1.00
25.48
O


ATOM
10416
C
SER
B
120
65.800
59.632
−23.581
1.00
25.73
C


ATOM
10417
O
SER
B
120
65.824
60.162
−24.688
1.00
26.49
O


ATOM
10419
N
GLY
B
121
66.839
59.636
−22.753
1.00
26.46
N


ATOM
10420
CA
GLY
B
121
68.092
60.312
−23.081
1.00
27.28
C


ATOM
10423
C
GLY
B
121
68.813
59.815
−24.327
1.00
28.31
C


ATOM
10424
O
GLY
B
121
69.723
60.485
−24.819
1.00
28.79
O


ATOM
10426
N
PHE
B
122
68.421
58.645
−24.834
1.00
29.36
N


ATOM
10427
CA
PHE
B
122
69.051
58.052
−26.022
1.00
30.12
C


ATOM
10429
CB
PHE
B
122
69.321
56.565
−25.779
1.00
29.81
C


ATOM
10432
CG
PHE
B
122
70.054
56.285
−24.499
1.00
27.48
C


ATOM
10433
CD1
PHE
B
122
71.432
56.437
−24.427
1.00
26.82
C


ATOM
10435
CE1
PHE
B
122
72.118
56.179
−23.247
1.00
27.23
C


ATOM
10437
CZ
PHE
B
122
71.424
55.765
−22.119
1.00
27.22
C


ATOM
10439
CE2
PHE
B
122
70.045
55.609
−22.177
1.00
28.12
C


ATOM
10441
CD2
PHE
B
122
69.368
55.870
−23.366
1.00
28.41
C


ATOM
10443
C
PHE
B
122
68.221
58.225
−27.301
1.00
31.15
C


ATOM
10444
O
PHE
B
122
68.514
57.596
−28.320
1.00
30.71
O


ATOM
10446
N
LYS
B
123
67.194
59.073
−27.242
1.00
32.65
N


ATOM
10447
CA
LYS
B
123
66.355
59.370
−28.401
1.00
33.99
C


ATOM
10449
CB
LYS
B
123
64.872
59.295
−28.023
1.00
34.60
C


ATOM
10452
CG
LYS
B
123
64.341
57.859
−27.935
1.00
37.48
C


ATOM
10455
CD
LYS
B
123
63.127
57.727
−27.014
1.00
42.03
C


ATOM
10458
CE
LYS
B
123
61.829
58.160
−27.676
1.00
42.87
C


ATOM
10461
NZ
LYS
B
123
61.236
57.080
−28.516
1.00
44.11
N


ATOM
10465
C
LYS
B
123
66.710
60.743
−28.972
1.00
34.42
C


ATOM
10466
O
LYS
B
123
67.167
61.628
−28.245
1.00
34.43
O


ATOM
10468
N
ASP
B
124
66.500
60.906
−30.277
1.00
35.22
N


ATOM
10469
CA
ASP
B
124
66.917
62.118
−30.999
1.00
36.02
C


ATOM
10471
CB
ASP
B
124
67.408
61.758
−32.424
1.00
35.79
C


ATOM
10474
CG
ASP
B
124
66.304
61.198
−33.327
1.00
35.25
C


ATOM
10475
OD1
ASP
B
124
65.102
61.351
−33.015
1.00
34.10
O


ATOM
10476
OD2
ASP
B
124
66.652
60.592
−34.363
1.00
34.85
O


ATOM
10477
C
ASP
B
124
65.823
63.201
−31.027
1.00
36.71
C


ATOM
10478
O
ASP
B
124
64.827
63.111
−30.305
1.00
36.49
O


ATOM
10480
N
GLN
B
125
66.033
64.223
−31.857
1.00
37.90
N


ATOM
10481
CA
GLN
B
125
65.081
65.331
−32.048
1.00
38.69
C


ATOM
10483
CB
GLN
B
125
65.479
66.158
−33.284
1.00
38.91
C


ATOM
10486
CG
GLN
B
125
66.900
66.755
−33.251
1.00
40.89
C


ATOM
10489
CD
GLN
B
125
66.940
68.279
−33.183
1.00
43.09
C


ATOM
10490
OE1
GLN
B
125
65.978
68.965
−33.531
1.00
44.49
O


ATOM
10491
NE2
GLN
B
125
68.074
68.814
−32.746
1.00
43.53
N


ATOM
10494
C
GLN
B
125
63.626
64.865
−32.222
1.00
38.47
C


ATOM
10495
O
GLN
B
125
62.710
65.437
−31.625
1.00
38.32
O


ATOM
10497
N
ASN
B
126
63.433
63.821
−33.029
1.00
38.16
N


ATOM
10498
CA
ASN
B
126
62.099
63.376
−33.454
1.00
37.55
C


ATOM
10500
CB
ASN
B
126
62.119
63.054
−34.953
1.00
37.83
C


ATOM
10503
CG
ASN
B
126
62.911
64.069
−35.759
1.00
38.62
C


ATOM
10504
OD1
ASN
B
126
62.735
65.278
−35.606
1.00
39.51
O


ATOM
10505
ND2
ASN
B
126
63.794
63.577
−36.620
1.00
37.66
N


ATOM
10508
C
ASN
B
126
61.569
62.158
−32.688
1.00
36.58
C


ATOM
10509
O
ASN
B
126
60.651
61.481
−33.154
1.00
35.56
O


ATOM
10511
N
GLY
B
127
62.147
61.881
−31.520
1.00
36.10
N


ATOM
10512
CA
GLY
B
127
61.726
60.748
−30.692
1.00
35.68
C


ATOM
10515
C
GLY
B
127
62.149
59.386
−31.224
1.00
35.16
C


ATOM
10516
O
GLY
B
127
61.557
58.363
−30.863
1.00
34.95
O


ATOM
10518
N
ASN
B
128
63.168
59.373
−32.082
1.00
34.38
N


ATOM
10519
CA
ASN
B
128
63.747
58.133
−32.594
1.00
33.52
C


ATOM
10521
CB
ASN
B
128
63.949
58.223
−34.108
1.00
33.53
C


ATOM
10524
CG
ASN
B
128
62.639
58.247
−34.872
1.00
33.33
C


ATOM
10525
OD1
ASN
B
128
61.733
57.456
−34.602
1.00
33.76
O


ATOM
10526
ND2
ASN
B
128
62.535
59.152
−35.837
1.00
32.74
N


ATOM
10529
C
ASN
B
128
65.078
57.845
−31.907
1.00
32.50
C


ATOM
10530
O
ASN
B
128
65.786
58.766
−31.502
1.00
32.13
O


ATOM
10532
N
PHE
B
129
65.413
56.566
−31.770
1.00
31.65
N


ATOM
10533
CA
PHE
B
129
66.679
56.173
−31.154
1.00
30.65
C


ATOM
10535
CB
PHE
B
129
66.744
54.650
−30.954
1.00
30.52
C


ATOM
10538
CG
PHE
B
129
65.924
54.155
−29.791
1.00
27.36
C


ATOM
10539
CD1
PHE
B
129
66.433
54.196
−28.502
1.00
26.85
C


ATOM
10541
CE1
PHE
B
129
65.683
53.750
−27.422
1.00
25.98
C


ATOM
10543
CZ
PHE
B
129
64.405
53.255
−27.622
1.00
26.31
C


ATOM
10545
CE2
PHE
B
129
63.882
53.207
−28.899
1.00
26.76
C


ATOM
10547
CD2
PHE
B
129
64.645
53.661
−29.981
1.00
27.19
C


ATOM
10549
C
PHE
B
129
67.845
56.664
−32.012
1.00
30.53
C


ATOM
10550
O
PHE
B
129
67.877
56.421
−33.218
1.00
30.46
O


ATOM
10552
N
LEU
B
130
68.782
57.372
−31.379
1.00
30.69
N


ATOM
10553
CA
LEU
B
130
69.953
57.939
−32.058
1.00
30.52
C


ATOM
10555
CB
LEU
B
130
70.924
58.543
−31.034
1.00
30.31
C


ATOM
10558
CG
LEU
B
130
70.550
59.935
−30.506
1.00
31.18
C


ATOM
10560
CD1
LEU
B
130
71.216
60.226
−29.164
1.00
30.08
C


ATOM
10564
CD2
LEU
B
130
70.905
61.026
−31.528
1.00
30.64
C


ATOM
10568
C
LEU
B
130
70.674
56.903
−32.922
1.00
30.82
C


ATOM
10569
O
LEU
B
130
70.952
55.796
−32.473
1.00
30.38
O


ATOM
10571
N
GLU
B
131
70.977
57.286
−34.159
1.00
32.16
N


ATOM
10572
CA
GLU
B
131
71.503
56.365
−35.168
1.00
33.12
C


ATOM
10574
CB
GLU
B
131
71.568
57.065
−36.538
1.00
33.71
C


ATOM
10577
CG
GLU
B
131
71.897
56.151
−37.733
1.00
36.43
C


ATOM
10580
CD
GLU
B
131
70.710
55.319
−38.223
1.00
40.75
C


ATOM
10581
OE1
GLU
B
131
69.735
55.119
−37.462
1.00
41.45
O


ATOM
10582
OE2
GLU
B
131
70.761
54.855
−39.383
1.00
43.48
O


ATOM
10583
C
GLU
B
131
72.879
55.799
−34.811
1.00
33.09
C


ATOM
10584
O
GLU
B
131
73.115
54.606
−34.992
1.00
33.35
O


ATOM
10586
N
ASN
B
132
73.779
56.641
−34.304
1.00
32.84
N


ATOM
10587
CA
ASN
B
132
75.158
56.209
−34.023
1.00
33.04
C


ATOM
10589
CB
ASN
B
132
76.092
57.424
−33.821
1.00
33.50
C


ATOM
10592
CG
ASN
B
132
75.826
58.187
−32.522
1.00
35.16
C


ATOM
10593
OD1
ASN
B
132
75.156
57.695
−31.610
1.00
39.01
O


ATOM
10594
ND2
ASN
B
132
76.369
59.396
−32.437
1.00
36.19
N


ATOM
10597
C
ASN
B
132
75.312
55.196
−32.869
1.00
32.26
C


ATOM
10598
O
ASN
B
132
76.409
54.699
−32.623
1.00
31.64
O


ATOM
10600
N
LEU
B
133
74.217
54.900
−32.170
1.00
32.21
N


ATOM
10601
CA
LEU
B
133
74.195
53.828
−31.166
1.00
32.15
C


ATOM
10603
CB
LEU
B
133
72.879
53.847
−30.373
1.00
31.75
C


ATOM
10606
CG
LEU
B
133
72.699
54.968
−29.346
1.00
30.29
C


ATOM
10608
CD1
LEU
B
133
71.251
55.031
−28.881
1.00
28.69
C


ATOM
10612
CD2
LEU
B
133
73.638
54.778
−28.164
1.00
25.64
C


ATOM
10616
C
LEU
B
133
74.397
52.434
−31.774
1.00
32.15
C


ATOM
10617
O
LEU
B
133
74.749
51.494
−31.061
1.00
32.64
O


ATOM
10619
N
LYS
B
134
74.174
52.301
−33.079
1.00
31.79
N


ATOM
10620
CA
LYS
B
134
74.377
51.030
−33.773
1.00
32.43
C


ATOM
10622
CB
LYS
B
134
73.833
51.106
−35.206
1.00
32.65
C


ATOM
10625
CG
LYS
B
134
74.690
51.920
−36.177
1.00
36.00
C


ATOM
10628
CD
LYS
B
134
73.923
52.234
−37.451
1.00
36.62
C


ATOM
10631
CE
LYS
B
134
74.789
52.877
−38.511
1.00
36.99
C


ATOM
10634
NZ
LYS
B
134
73.959
53.264
−39.689
1.00
37.62
N


ATOM
10638
C
LYS
B
134
75.842
50.585
−33.793
1.00
32.51
C


ATOM
10639
O
LYS
B
134
76.126
49.406
−33.988
1.00
32.95
O


ATOM
10641
N
GLU
B
135
76.765
51.524
−33.592
1.00
32.81
N


ATOM
10642
CA
GLU
B
135
78.191
51.200
−33.495
1.00
33.04
C


ATOM
10644
CB
GLU
B
135
79.042
52.470
−33.565
1.00
33.88
C


ATOM
10647
CG
GLU
B
135
78.901
53.249
−34.873
1.00
37.74
C


ATOM
10650
CD
GLU
B
135
79.689
54.550
−34.872
1.00
43.53
C


ATOM
10651
OE1
GLU
B
135
80.633
54.690
−34.057
1.00
43.50
O


ATOM
10652
OE2
GLU
B
135
79.365
55.435
−35.698
1.00
47.71
O


ATOM
10653
C
GLU
B
135
78.534
50.442
−32.215
1.00
32.14
C


ATOM
10654
O
GLU
B
135
79.621
49.875
−32.113
1.00
32.81
O


ATOM
10656
N
ASP
B
136
77.621
50.453
−31.239
1.00
30.60
N


ATOM
10657
CA
ASP
B
136
77.782
49.695
−30.000
1.00
29.16
C


ATOM
10659
CB
ASP
B
136
77.489
50.593
−28.794
1.00
29.30
C


ATOM
10662
CG
ASP
B
136
77.839
49.938
−27.468
1.00
31.46
C


ATOM
10663
OD1
ASP
B
136
78.540
48.901
−27.456
1.00
35.77
O


ATOM
10664
OD2
ASP
B
136
77.406
50.466
−26.425
1.00
35.43
O


ATOM
10665
C
ASP
B
136
76.859
48.471
−30.010
1.00
27.89
C


ATOM
10666
O
ASP
B
136
75.735
48.513
−29.506
1.00
27.26
O


ATOM
10668
N
ILE
B
137
77.356
47.379
−30.583
1.00
26.87
N


ATOM
10669
CA
ILE
B
137
76.580
46.148
−30.737
1.00
26.04
C


ATOM
10671
CB
ILE
B
137
77.390
45.066
−31.487
1.00
25.62
C


ATOM
10673
CG1
ILE
B
137
77.815
45.559
−32.877
1.00
27.86
C


ATOM
10676
CD1
ILE
B
137
76.657
45.925
−33.792
1.00
29.16
C


ATOM
10680
CG2
ILE
B
137
76.584
43.792
−31.633
1.00
24.19
C


ATOM
10684
C
ILE
B
137
76.146
45.591
−29.382
1.00
26.70
C


ATOM
10685
O
ILE
B
137
75.042
45.071
−29.242
1.00
27.81
O


ATOM
10687
N
LYS
B
138
77.026
45.706
−28.392
1.00
27.23
N


ATOM
10688
CA
LYS
B
138
76.743
45.284
−27.020
1.00
26.65
C


ATOM
10690
CB
LYS
B
138
77.948
45.605
−26.123
1.00
27.89
C


ATOM
10693
CG
LYS
B
138
78.281
44.564
−25.065
1.00
31.52
C


ATOM
10696
CD
LYS
B
138
79.355
45.109
−24.115
1.00
38.18
C


ATOM
10699
CE
LYS
B
138
79.767
44.111
−23.024
1.00
40.71
C


ATOM
10702
NZ
LYS
B
138
81.165
43.608
−23.172
1.00
39.68
N


ATOM
10706
C
LYS
B
138
75.487
45.990
−26.491
1.00
25.04
C


ATOM
10707
O
LYS
B
138
74.595
45.347
−25.921
1.00
24.33
O


ATOM
10709
N
ALA
B
139
75.425
47.310
−26.685
1.00
23.67
N


ATOM
10710
CA
ALA
B
139
74.258
48.110
−26.280
1.00
23.10
C


ATOM
10712
CB
ALA
B
139
74.537
49.605
−26.450
1.00
22.12
C


ATOM
10716
C
ALA
B
139
72.996
47.715
−27.059
1.00
22.42
C


ATOM
10717
O
ALA
B
139
71.910
47.588
−26.486
1.00
22.30
O


ATOM
10719
N
ILE
B
140
73.150
47.523
−28.364
1.00
21.67
N


ATOM
10720
CA
ILE
B
140
72.043
47.111
−29.216
1.00
20.72
C


ATOM
10722
CB
ILE
B
140
72.481
47.013
−30.693
1.00
20.38
C


ATOM
10724
CG1
ILE
B
140
72.768
48.406
−31.249
1.00
20.37
C


ATOM
10727
CD1
ILE
B
140
71.550
49.299
−31.327
1.00
17.94
C


ATOM
10731
CG2
ILE
B
140
71.410
46.343
−31.535
1.00
20.43
C


ATOM
10735
C
ILE
B
140
71.465
45.781
−28.740
1.00
20.20
C


ATOM
10736
O
ILE
B
140
70.243
45.630
−28.647
1.00
20.13
O


ATOM
10738
N
LEU
B
141
72.341
44.832
−28.419
1.00
19.56
N


ATOM
10739
CA
LEU
B
141
71.900
43.532
−27.903
1.00
19.42
C


ATOM
10741
CB
LEU
B
141
73.076
42.564
−27.736
1.00
18.92
C


ATOM
10744
CG
LEU
B
141
73.594
41.924
−29.025
1.00
20.88
C


ATOM
10746
CD1
LEU
B
141
74.872
41.159
−28.749
1.00
18.48
C


ATOM
10750
CD2
LEU
B
141
72.543
41.010
−29.649
1.00
19.79
C


ATOM
10754
C
LEU
B
141
71.141
43.649
−26.583
1.00
18.99
C


ATOM
10755
O
LEU
B
141
70.174
42.928
−26.372
1.00
18.79
O


ATOM
10757
N
SER
B
142
71.576
44.549
−25.701
1.00
18.99
N


ATOM
10758
CA
SER
B
142
70.907
44.738
−24.409
1.00
18.94
C


ATOM
10760
CB
SER
B
142
71.745
45.601
−23.468
1.00
18.67
C


ATOM
10763
OG
SER
B
142
72.985
44.989
−23.197
1.00
23.20
O


ATOM
10765
C
SER
B
142
69.542
45.377
−24.598
1.00
18.42
C


ATOM
10766
O
SER
B
142
68.583
45.031
−23.903
1.00
18.43
O


ATOM
10768
N
LEU
B
143
69.460
46.314
−25.537
1.00
17.73
N


ATOM
10769
CA
LEU
B
143
68.187
46.919
−25.887
1.00
17.79
C


ATOM
10771
CB
LEU
B
143
68.397
48.034
−26.912
1.00
17.79
C


ATOM
10774
CG
LEU
B
143
67.188
48.927
−27.208
1.00
18.37
C


ATOM
10776
CD1
LEU
B
143
66.690
49.626
−25.948
1.00
15.94
C


ATOM
10780
CD2
LEU
B
143
67.541
49.937
−28.294
1.00
13.43
C


ATOM
10784
C
LEU
B
143
67.240
45.840
−26.426
1.00
17.63
C


ATOM
10785
O
LEU
B
143
66.132
45.662
−25.909
1.00
17.09
O


ATOM
10787
N
TYR
B
144
67.697
45.105
−27.441
1.00
16.96
N


ATOM
10788
CA
TYR
B
144
66.934
43.989
−27.999
1.00
16.71
C


ATOM
10790
CB
TYR
B
144
67.793
43.179
−28.979
1.00
16.77
C


ATOM
10793
CG
TYR
B
144
67.160
41.896
−29.497
1.00
16.66
C


ATOM
10794
CD1
TYR
B
144
66.195
41.920
−30.500
1.00
18.99
C


ATOM
10796
CE1
TYR
B
144
65.617
40.733
−30.981
1.00
18.24
C


ATOM
10798
CZ
TYR
B
144
66.018
39.515
−30.457
1.00
20.28
C


ATOM
10799
OH
TYR
B
144
65.455
38.344
−30.921
1.00
22.86
O


ATOM
10801
CE2
TYR
B
144
66.982
39.470
−29.461
1.00
18.13
C


ATOM
10803
CD2
TYR
B
144
67.544
40.654
−28.990
1.00
20.74
C


ATOM
10805
C
TYR
B
144
66.412
43.093
−26.887
1.00
16.99
C


ATOM
10806
O
TYR
B
144
65.208
42.845
−26.799
1.00
18.37
O


ATOM
10808
N
GLU
B
145
67.309
42.636
−26.019
1.00
16.70
N


ATOM
10809
CA
GLU
B
145
66.928
41.699
−24.956
1.00
17.05
C


ATOM
10811
CB
GLU
B
145
68.162
41.137
−24.248
1.00
17.15
C


ATOM
10814
CG
GLU
B
145
69.093
40.339
−25.162
1.00
18.79
C


ATOM
10817
CD
GLU
B
145
68.601
38.946
−25.483
1.00
23.46
C


ATOM
10818
OE1
GLU
B
145
67.435
38.606
−25.169
1.00
26.98
O


ATOM
10819
OE2
GLU
B
145
69.400
38.176
−26.054
1.00
29.40
O


ATOM
10820
C
GLU
B
145
65.964
42.304
−23.934
1.00
16.69
C


ATOM
10821
O
GLU
B
145
65.086
41.602
−23.434
1.00
17.65
O


ATOM
10823
N
ALA
B
146
66.115
43.594
−23.635
1.00
16.19
N


ATOM
10824
CA
ALA
B
146
65.214
44.278
−22.696
1.00
15.30
C


ATOM
10826
CB
ALA
B
146
65.763
45.640
−22.314
1.00
14.49
C


ATOM
10830
C
ALA
B
146
63.802
44.425
−23.266
1.00
15.06
C


ATOM
10831
O
ALA
B
146
62.816
44.303
−22.531
1.00
14.47
O


ATOM
10833
N
SER
B
147
63.707
44.667
−24.570
1.00
14.70
N


ATOM
10834
CA
SER
B
147
62.422
44.946
−25.205
1.00
15.84
C


ATOM
10836
CB
SER
B
147
62.589
45.147
−26.718
1.00
15.84
C


ATOM
10839
OG
SER
B
147
62.818
43.920
−27.383
1.00
17.77
O


ATOM
10841
C
SER
B
147
61.386
43.851
−24.931
1.00
17.09
C


ATOM
10842
O
SER
B
147
60.203
44.138
−24.734
1.00
18.31
O


ATOM
10844
N
PHE
B
148
61.833
42.600
−24.878
1.00
17.25
N


ATOM
10845
CA
PHE
B
148
60.922
41.484
−24.649
1.00
16.14
C


ATOM
10847
CB
PHE
B
148
61.609
40.154
−24.964
1.00
15.49
C


ATOM
10850
CG
PHE
B
148
61.971
40.012
−26.407
1.00
14.49
C


ATOM
10851
CD1
PHE
B
148
61.050
39.541
−27.324
1.00
14.20
C


ATOM
10853
CE1
PHE
B
148
61.381
39.431
−28.672
1.00
14.58
C


ATOM
10855
CZ
PHE
B
148
62.629
39.814
−29.115
1.00
11.47
C


ATOM
10857
CE2
PHE
B
148
63.553
40.296
−28.208
1.00
15.42
C


ATOM
10859
CD2
PHE
B
148
63.220
40.398
−26.861
1.00
13.40
C


ATOM
10861
C
PHE
B
148
60.314
41.456
−23.255
1.00
16.76
C


ATOM
10862
O
PHE
B
148
59.461
40.612
−22.988
1.00
18.26
O


ATOM
10864
N
LEU
B
149
60.725
42.365
−22.369
1.00
17.06
N


ATOM
10865
CA
LEU
B
149
60.083
42.475
−21.045
1.00
17.69
C


ATOM
10867
CB
LEU
B
149
61.120
42.731
−19.937
1.00
16.45
C


ATOM
10870
CG
LEU
B
149
61.863
41.475
−19.453
1.00
16.92
C


ATOM
10872
CD1
LEU
B
149
62.801
40.939
−20.521
1.00
9.82
C


ATOM
10876
CD2
LEU
B
149
62.634
41.738
−18.158
1.00
14.46
C


ATOM
10880
C
LEU
B
149
58.978
43.545
−21.042
1.00
18.44
C


ATOM
10881
O
LEU
B
149
58.475
43.927
−19.983
1.00
19.20
O


ATOM
10883
N
ALA
B
150
58.585
43.998
−22.232
1.00
18.19
N


ATOM
10884
CA
ALA
B
150
57.581
45.050
−22.368
1.00
17.96
C


ATOM
10886
CB
ALA
B
150
57.379
45.412
−23.832
1.00
16.76
C


ATOM
10890
C
ALA
B
150
56.251
44.653
−21.755
1.00
18.74
C


ATOM
10891
O
ALA
B
150
55.888
43.474
−21.734
1.00
19.06
O


ATOM
10893
N
LEU
B
151
55.541
45.655
−21.248
1.00
18.68
N


ATOM
10894
CA
LEU
B
151
54.165
45.496
−20.819
1.00
19.10
C


ATOM
10896
CB
LEU
B
151
53.954
46.127
−19.442
1.00
19.19
C


ATOM
10899
CG
LEU
B
151
54.793
45.595
−18.277
1.00
19.03
C


ATOM
10901
CD1
LEU
B
151
54.565
46.447
−17.023
1.00
15.48
C


ATOM
10905
CD2
LEU
B
151
54.485
44.130
−18.008
1.00
11.45
C


ATOM
10909
C
LEU
B
151
53.285
46.189
−21.849
1.00
19.15
C


ATOM
10910
O
LEU
B
151
53.782
46.932
−22.702
1.00
19.79
O


ATOM
10912
N
GLU
B
152
51.979
45.952
−21.783
1.00
18.56
N


ATOM
10913
CA
GLU
B
152
51.060
46.650
−22.672
1.00
17.83
C


ATOM
10915
CB
GLU
B
152
49.606
46.302
−22.369
1.00
17.60
C


ATOM
10918
CG
GLU
B
152
49.134
45.017
−23.013
1.00
21.05
C


ATOM
10921
CD
GLU
B
152
47.628
44.969
−23.136
1.00
23.21
C


ATOM
10922
OE1
GLU
B
152
46.987
44.275
−22.317
1.00
24.13
O


ATOM
10923
OE2
GLU
B
152
47.091
45.649
−24.037
1.00
22.06
O


ATOM
10924
C
GLU
B
152
51.253
48.148
−22.540
1.00
17.26
C


ATOM
10925
O
GLU
B
152
51.424
48.665
−21.435
1.00
17.17
O


ATOM
10927
N
GLY
B
153
51.236
48.833
−23.678
1.00
17.21
N


ATOM
10928
CA
GLY
B
153
51.340
50.284
−23.708
1.00
16.98
C


ATOM
10931
C
GLY
B
153
52.751
50.832
−23.609
1.00
17.22
C


ATOM
10932
O
GLY
B
153
52.931
52.043
−23.609
1.00
18.36
O


ATOM
10934
N
GLU
B
154
53.758
49.965
−23.520
1.00
17.26
N


ATOM
10935
CA
GLU
B
154
55.147
50.426
−23.521
1.00
16.95
C


ATOM
10937
CB
GLU
B
154
56.031
49.548
−22.625
1.00
16.80
C


ATOM
10940
CG
GLU
B
154
55.745
49.761
−21.141
1.00
16.73
C


ATOM
10943
CD
GLU
B
154
56.617
48.921
−20.222
1.00
18.67
C


ATOM
10944
OE1
GLU
B
154
56.961
47.776
−20.586
1.00
15.50
O


ATOM
10945
OE2
GLU
B
154
56.946
49.401
−19.116
1.00
16.67
O


ATOM
10946
C
GLU
B
154
55.652
50.482
−24.959
1.00
16.64
C


ATOM
10947
O
GLU
B
154
56.440
49.646
−25.403
1.00
17.07
O


ATOM
10949
N
ASN
B
155
55.168
51.490
−25.673
1.00
16.57
N


ATOM
10950
CA
ASN
B
155
55.489
51.705
−27.087
1.00
17.00
C


ATOM
10952
CB
ASN
B
155
54.658
52.874
−27.647
1.00
16.55
C


ATOM
10955
CG
ASN
B
155
54.862
54.169
−26.862
1.00
17.46
C


ATOM
10956
OD1
ASN
B
155
54.556
54.244
−25.665
1.00
16.21
O


ATOM
10957
ND2
ASN
B
155
55.379
55.195
−27.534
1.00
15.36
N


ATOM
10960
C
ASN
B
155
56.978
51.947
−27.374
1.00
17.12
C


ATOM
10961
O
ASN
B
155
57.455
51.623
−28.464
1.00
17.70
O


ATOM
10963
N
ILE
B
156
57.705
52.514
−26.410
1.00
16.75
N


ATOM
10964
CA
ILE
B
156
59.142
52.751
−26.579
1.00
17.02
C


ATOM
10966
CB
ILE
B
156
59.747
53.516
−25.394
1.00
16.98
C


ATOM
10968
CG1
ILE
B
156
59.209
54.944
−25.344
1.00
18.01
C


ATOM
10971
CD1
ILE
B
156
59.759
55.743
−24.174
1.00
22.11
C


ATOM
10975
CG2
ILE
B
156
61.263
53.528
−25.489
1.00
15.85
C


ATOM
10979
C
ILE
B
156
59.890
51.431
−26.701
1.00
17.81
C


ATOM
10980
O
ILE
B
156
60.736
51.265
−27.584
1.00
18.73
O


ATOM
10982
N
LEU
B
157
59.588
50.502
−25.800
1.00
17.50
N


ATOM
10983
CA
LEU
B
157
60.209
49.189
−25.841
1.00
17.85
C


ATOM
10985
CB
LEU
B
157
59.764
48.326
−24.649
1.00
18.12
C


ATOM
10988
CG
LEU
B
157
60.213
48.793
−23.257
1.00
16.86
C


ATOM
10990
CD1
LEU
B
157
59.785
47.793
−22.187
1.00
15.12
C


ATOM
10994
CD2
LEU
B
157
61.715
49.001
−23.211
1.00
14.35
C


ATOM
10998
C
LEU
B
157
59.886
48.494
−27.159
1.00
18.64
C


ATOM
10999
O
LEU
B
157
60.739
47.810
−27.718
1.00
18.33
O


ATOM
11001
N
ASP
B
158
58.660
48.677
−27.656
1.00
19.51
N


ATOM
11002
CA
ASP
B
158
58.270
48.138
−28.965
1.00
19.82
C


ATOM
11004
CB
ASP
B
158
56.789
48.399
−29.255
1.00
19.82
C


ATOM
11007
CG
ASP
B
158
55.874
47.369
−28.617
1.00
22.44
C


ATOM
11008
OD1
ASP
B
158
56.302
46.678
−27.666
1.00
25.77
O


ATOM
11009
OD2
ASP
B
158
54.713
47.259
−29.068
1.00
24.60
O


ATOM
11010
C
ASP
B
158
59.124
48.732
−30.078
1.00
19.74
C


ATOM
11011
O
ASP
B
158
59.614
48.012
−30.943
1.00
19.23
O


ATOM
11013
N
GLU
B
159
59.300
50.049
−30.044
1.00
20.53
N


ATOM
11014
CA
GLU
B
159
60.181
50.735
−30.989
1.00
21.17
C


ATOM
11016
CB
GLU
B
159
60.149
52.248
−30.754
1.00
22.23
C


ATOM
11019
CG
GLU
B
159
58.810
52.912
−31.059
1.00
25.93
C


ATOM
11022
CD
GLU
B
159
58.660
54.274
−30.385
1.00
32.63
C


ATOM
11023
OE1
GLU
B
159
59.692
54.914
−30.047
1.00
33.46
O


ATOM
11024
OE2
GLU
B
159
57.496
54.703
−30.199
1.00
34.47
O


ATOM
11025
C
GLU
B
159
61.619
50.239
−30.850
1.00
20.42
C


ATOM
11026
O
GLU
B
159
62.309
50.018
−31.846
1.00
20.71
O


ATOM
11028
N
ALA
B
160
62.058
50.070
−29.605
1.00
19.27
N


ATOM
11029
CA
ALA
B
160
63.400
49.585
−29.318
1.00
18.91
C


ATOM
11031
CB
ALA
B
160
63.607
49.463
−27.821
1.00
18.45
C


ATOM
11035
C
ALA
B
160
63.647
48.245
−29.996
1.00
18.88
C


ATOM
11036
O
ALA
B
160
64.690
48.037
−30.600
1.00
19.92
O


ATOM
11038
N
LYS
B
161
62.681
47.342
−29.907
1.00
19.49
N


ATOM
11039
CA
LYS
B
161
62.806
46.044
−30.549
1.00
20.61
C


ATOM
11041
CB
LYS
B
161
61.551
45.185
−30.337
1.00
20.62
C


ATOM
11044
CG
LYS
B
161
61.745
43.722
−30.728
1.00
23.49
C


ATOM
11047
CD
LYS
B
161
60.437
42.937
−30.813
1.00
26.14
C


ATOM
11050
CE
LYS
B
161
59.926
42.500
−29.450
1.00
27.36
C


ATOM
11053
NZ
LYS
B
161
58.817
41.518
−29.583
1.00
28.82
N


ATOM
11057
C
LYS
B
161
63.067
46.246
−32.033
1.00
21.10
C


ATOM
11058
O
LYS
B
161
64.101
45.824
−32.542
1.00
22.42
O


ATOM
11060
N
VAL
B
162
62.142
46.922
−32.711
1.00
21.17
N


ATOM
11061
CA
VAL
B
162
62.239
47.144
−34.156
1.00
20.73
C


ATOM
11063
CB
VAL
B
162
61.091
48.044
−34.675
1.00
21.27
C


ATOM
11065
CG1
VAL
B
162
61.278
48.367
−36.156
1.00
19.53
C


ATOM
11069
CG2
VAL
B
162
59.746
47.371
−34.439
1.00
18.18
C


ATOM
11073
C
VAL
B
162
63.582
47.767
−34.524
1.00
20.33
C


ATOM
11074
O
VAL
B
162
64.262
47.293
−35.431
1.00
20.38
O


ATOM
11076
N
PHE
B
163
63.952
48.819
−33.800
1.00
20.40
N


ATOM
11077
CA
PHE
B
163
65.227
49.517
−34.000
1.00
20.69
C


ATOM
11079
CB
PHE
B
163
65.350
50.654
−32.982
1.00
20.35
C


ATOM
11082
CG
PHE
B
163
66.678
51.367
−33.003
1.00
19.94
C


ATOM
11083
CD1
PHE
B
163
66.935
52.361
−33.938
1.00
18.75
C


ATOM
11085
CE1
PHE
B
163
68.153
53.031
−33.949
1.00
16.42
C


ATOM
11087
CZ
PHE
B
163
69.125
52.716
−33.013
1.00
16.18
C


ATOM
11089
CE2
PHE
B
163
68.879
51.737
−32.068
1.00
17.55
C


ATOM
11091
CD2
PHE
B
163
67.658
51.072
−32.060
1.00
18.96
C


ATOM
11093
C
PHE
B
163
66.400
48.559
−33.861
1.00
21.43
C


ATOM
11094
O
PHE
B
163
67.276
48.495
−34.724
1.00
21.26
O


ATOM
11096
N
ALA
B
164
66.402
47.809
−32.767
1.00
22.69
N


ATOM
11097
CA
ALA
B
164
67.482
46.877
−32.481
1.00
23.59
C


ATOM
11099
CB
ALA
B
164
67.299
46.253
−31.094
1.00
22.57
C


ATOM
11103
C
ALA
B
164
67.571
45.795
−33.558
1.00
24.62
C


ATOM
11104
O
ALA
B
164
68.654
45.541
−34.094
1.00
24.65
O


ATOM
11106
N
ILE
B
165
66.436
45.177
−33.888
1.00
25.49
N


ATOM
11107
CA
ILE
B
165
66.429
44.065
−34.847
1.00
26.70
C


ATOM
11109
CB
ILE
B
165
65.033
43.417
−35.013
1.00
26.48
C


ATOM
11111
CG1
ILE
B
165
64.549
42.810
−33.701
1.00
27.74
C


ATOM
11114
CD1
ILE
B
165
63.187
42.148
−33.801
1.00
29.76
C


ATOM
11118
CG2
ILE
B
165
65.082
42.297
−36.042
1.00
26.39
C


ATOM
11122
C
ILE
B
165
66.931
44.513
−36.222
1.00
28.02
C


ATOM
11123
O
ILE
B
165
67.757
43.833
−36.832
1.00
28.59
O


ATOM
11125
N
SER
B
166
66.451
45.661
−36.698
1.00
28.72
N


ATOM
11126
CA
SER
B
166
66.789
46.123
−38.044
1.00
29.28
C


ATOM
11128
CB
SER
B
166
66.049
47.429
−38.387
1.00
29.30
C


ATOM
11131
OG
SER
B
166
66.376
48.478
−37.493
1.00
28.32
O


ATOM
11133
C
SER
B
166
68.300
46.284
−38.226
1.00
30.17
C


ATOM
11134
O
SER
B
166
68.831
46.042
−39.312
1.00
30.00
O


ATOM
11136
N
HIS
B
167
68.991
46.670
−37.158
1.00
31.31
N


ATOM
11137
CA
HIS
B
167
70.438
46.875
−37.223
1.00
32.64
C


ATOM
11139
CB
HIS
B
167
70.862
48.000
−36.270
1.00
32.70
C


ATOM
11142
CG
HIS
B
167
70.457
49.361
−36.756
1.00
39.52
C


ATOM
11143
ND1
HIS
B
167
71.350
50.400
−36.916
1.00
42.89
N


ATOM
11145
CE1
HIS
B
167
70.711
51.460
−37.381
1.00
42.59
C


ATOM
11147
NE2
HIS
B
167
69.441
51.142
−37.549
1.00
41.80
N


ATOM
11149
CD2
HIS
B
167
69.258
49.833
−37.175
1.00
43.19
C


ATOM
11151
C
HIS
B
167
71.218
45.577
−36.997
1.00
32.89
C


ATOM
11152
O
HIS
B
167
72.192
45.321
−37.702
1.00
33.78
O


ATOM
11154
N
LEU
B
168
70.773
44.754
−36.047
1.00
32.82
N


ATOM
11155
CA
LEU
B
168
71.368
43.432
−35.810
1.00
32.63
C


ATOM
11157
CB
LEU
B
168
70.706
42.752
−34.602
1.00
32.18
C


ATOM
11160
CG
LEU
B
168
70.985
43.375
−33.232
1.00
29.18
C


ATOM
11162
CD1
LEU
B
168
69.986
42.892
−32.195
1.00
22.43
C


ATOM
11166
CD2
LEU
B
168
72.398
43.070
−32.789
1.00
24.79
C


ATOM
11170
C
LEU
B
168
71.251
42.502
−37.020
1.00
33.86
C


ATOM
11171
O
LEU
B
168
72.181
41.759
−37.328
1.00
33.62
O


ATOM
11173
N
LYS
B
169
70.106
42.544
−37.696
1.00
36.00
N


ATOM
11174
CA
LYS
B
169
69.822
41.627
−38.805
1.00
37.86
C


ATOM
11176
CB
LYS
B
169
68.366
41.781
−39.264
1.00
37.94
C


ATOM
11179
CG
LYS
B
169
67.911
40.724
−40.261
1.00
41.68
C


ATOM
11182
CD
LYS
B
169
66.387
40.736
−40.488
1.00
44.82
C


ATOM
11185
CE
LYS
B
169
65.644
39.757
−39.569
1.00
46.91
C


ATOM
11188
NZ
LYS
B
169
64.290
39.386
−40.107
1.00
45.39
N


ATOM
11192
C
LYS
B
169
70.786
41.811
−39.989
1.00
38.99
C


ATOM
11193
O
LYS
B
169
71.134
40.840
−40.665
1.00
38.82
O


ATOM
11195
N
GLU
B
170
71.222
43.047
−40.224
1.00
40.23
N


ATOM
11196
CA
GLU
B
170
72.152
43.340
−41.311
1.00
41.60
C


ATOM
11198
CB
GLU
B
170
71.698
44.582
−42.094
1.00
41.71
C


ATOM
11201
CG
GLU
B
170
70.342
44.437
−42.789
1.00
43.65
C


ATOM
11204
CD
GLU
B
170
70.341
43.390
−43.900
1.00
46.26
C


ATOM
11205
OE1
GLU
B
170
71.147
43.519
−44.846
1.00
48.74
O


ATOM
11206
OE2
GLU
B
170
69.524
42.444
−43.833
1.00
47.40
O


ATOM
11207
C
GLU
B
170
73.565
43.533
−40.766
1.00
42.62
C


ATOM
11208
O
GLU
B
170
74.179
44.584
−40.964
1.00
42.44
O


ATOM
11210
N
LEU
B
171
74.070
42.513
−40.072
1.00
43.83
N


ATOM
11211
CA
LEU
B
171
75.457
42.505
−39.597
1.00
44.73
C


ATOM
11213
CB
LEU
B
171
75.528
42.421
−38.068
1.00
44.06
C


ATOM
11216
CG
LEU
B
171
75.188
43.682
−37.266
1.00
43.28
C


ATOM
11218
CD1
LEU
B
171
75.547
43.471
−35.806
1.00
43.25
C


ATOM
11222
CD2
LEU
B
171
75.885
44.923
−37.801
1.00
40.54
C


ATOM
11226
C
LEU
B
171
76.232
41.345
−40.212
1.00
46.20
C


ATOM
11227
O
LEU
B
171
75.673
40.271
−40.464
1.00
46.15
O


ATOM
11229
N
SER
B
172
77.523
41.585
−40.449
1.00
47.65
N


ATOM
11230
CA
SER
B
172
78.430
40.597
−41.025
1.00
48.88
C


ATOM
11232
CB
SER
B
172
79.135
41.175
−42.256
1.00
49.37
C


ATOM
11235
OG
SER
B
172
78.205
41.711
−43.186
1.00
51.22
O


ATOM
11237
C
SER
B
172
79.477
40.189
−39.996
1.00
49.65
C


ATOM
11238
O
SER
B
172
80.018
41.039
−39.277
1.00
49.85
O


ATOM
11240
N
GLU
B
173
79.780
38.892
−39.950
1.00
50.22
N


ATOM
11241
CA
GLU
B
173
80.777
38.343
−39.023
1.00
50.43
C


ATOM
11243
CB
GLU
B
173
80.913
36.823
−39.238
1.00
50.69
C


ATOM
11246
CG
GLU
B
173
82.007
36.138
−38.405
1.00
51.84
C


ATOM
11249
CD
GLU
B
173
81.921
34.615
−38.424
1.00
53.22
C


ATOM
11250
OE1
GLU
B
173
81.216
34.051
−39.291
1.00
52.39
O


ATOM
11251
OE2
GLU
B
173
82.573
33.982
−37.566
1.00
54.62
O


ATOM
11252
C
GLU
B
173
82.148
39.019
−39.149
1.00
50.15
C


ATOM
11253
O
GLU
B
173
82.967
38.919
−38.241
1.00
49.68
O


ATOM
11255
N
GLU
B
174
82.383
39.707
−40.266
1.00
50.44
N


ATOM
11256
CA
GLU
B
174
83.688
40.301
−40.567
1.00
51.06
C


ATOM
11258
CB
GLU
B
174
83.883
40.405
−42.089
1.00
51.51
C


ATOM
11261
CG
GLU
B
174
83.578
39.113
−42.868
1.00
53.45
C


ATOM
11264
CD
GLU
B
174
84.277
37.887
−42.296
1.00
56.91
C


ATOM
11265
OE1
GLU
B
174
85.513
37.935
−42.112
1.00
57.85
O


ATOM
11266
OE2
GLU
B
174
83.592
36.873
−42.033
1.00
57.98
O


ATOM
11267
C
GLU
B
174
83.886
41.675
−39.918
1.00
50.70
C


ATOM
11268
O
GLU
B
174
84.989
41.999
−39.470
1.00
50.69
O


ATOM
11270
N
LYS
B
175
82.821
42.475
−39.872
1.00
50.21
N


ATOM
11271
CA
LYS
B
175
82.875
43.811
−39.264
1.00
49.68
C


ATOM
11273
CB
LYS
B
175
81.605
44.611
−39.581
1.00
50.24
C


ATOM
11276
CG
LYS
B
175
81.254
44.700
−41.066
1.00
52.51
C


ATOM
11279
CD
LYS
B
175
80.061
45.624
−41.313
1.00
54.12
C


ATOM
11282
CE
LYS
B
175
79.682
45.672
−42.790
1.00
54.57
C


ATOM
11285
NZ
LYS
B
175
80.815
46.127
−43.648
1.00
54.21
N


ATOM
11289
C
LYS
B
175
83.040
43.700
−37.749
1.00
48.49
C


ATOM
11290
O
LYS
B
175
83.704
44.525
−37.125
1.00
48.25
O


ATOM
11292
N
ILE
B
176
82.418
42.673
−37.175
1.00
47.54
N


ATOM
11293
CA
ILE
B
176
82.507
42.375
−35.744
1.00
46.56
C


ATOM
11295
CB
ILE
B
176
81.098
42.319
−35.091
1.00
46.07
C


ATOM
11297
CG1
ILE
B
176
80.266
41.150
−35.642
1.00
44.01
C


ATOM
11300
CD1
ILE
B
176
78.794
41.235
−35.303
1.00
40.86
C


ATOM
11304
CG2
ILE
B
176
80.368
43.638
−35.306
1.00
45.78
C


ATOM
11308
C
ILE
B
176
83.244
41.045
−35.563
1.00
46.35
C


ATOM
11309
O
ILE
B
176
83.653
40.427
−36.541
1.00
46.03
O


ATOM
11311
N
GLY
B
177
83.424
40.610
−34.319
1.00
46.30
N


ATOM
11312
CA
GLY
B
177
84.106
39.341
−34.046
1.00
46.19
C


ATOM
11315
C
GLY
B
177
83.267
38.118
−34.393
1.00
45.84
C


ATOM
11316
O
GLY
B
177
82.070
38.229
−34.664
1.00
46.23
O


ATOM
11318
N
LYS
B
178
83.900
36.948
−34.393
1.00
45.15
N


ATOM
11319
CA
LYS
B
178
83.173
35.686
−34.536
1.00
44.35
C


ATOM
11321
CB
LYS
B
178
84.135
34.510
−34.753
1.00
44.51
C


ATOM
11324
CG
LYS
B
178
83.465
33.144
−34.622
1.00
46.27
C


ATOM
11327
CD
LYS
B
178
84.165
32.063
−35.419
1.00
48.89
C


ATOM
11330
CE
LYS
B
178
83.334
30.785
−35.434
1.00
49.86
C


ATOM
11333
NZ
LYS
B
178
83.989
29.702
−36.218
1.00
50.30
N


ATOM
11337
C
LYS
B
178
82.293
35.427
−33.307
1.00
43.25
C


ATOM
11338
O
LYS
B
178
81.140
35.006
−33.440
1.00
42.98
O


ATOM
11340
N
GLU
B
179
82.836
35.675
−32.117
1.00
42.01
N


ATOM
11341
CA
GLU
B
179
82.088
35.437
−30.880
1.00
41.17
C


ATOM
11343
CB
GLU
B
179
83.010
35.467
−29.647
1.00
41.20
C


ATOM
11346
CG
GLU
B
179
83.403
36.847
−29.138
1.00
42.43
C


ATOM
11349
CD
GLU
B
179
84.257
36.773
−27.883
1.00
44.81
C


ATOM
11350
OE1
GLU
B
179
83.740
37.107
−26.794
1.00
45.22
O


ATOM
11351
OE2
GLU
B
179
85.438
36.367
−27.985
1.00
42.41
O


ATOM
11352
C
GLU
B
179
80.923
36.419
−30.733
1.00
39.82
C


ATOM
11353
O
GLU
B
179
79.893
36.082
−30.151
1.00
40.22
O


ATOM
11355
N
LEU
B
180
81.087
37.627
−31.265
1.00
38.19
N


ATOM
11356
CA
LEU
B
180
80.001
38.601
−31.301
1.00
36.94
C


ATOM
11358
CB
LEU
B
180
80.557
40.005
−31.562
1.00
36.89
C


ATOM
11361
CG
LEU
B
180
79.649
41.226
−31.340
1.00
37.50
C


ATOM
11363
CD1
LEU
B
180
78.489
40.940
−30.393
1.00
39.28
C


ATOM
11367
CD2
LEU
B
180
80.466
42.409
−30.827
1.00
37.31
C


ATOM
11371
C
LEU
B
180
78.961
38.205
−32.359
1.00
35.94
C


ATOM
11372
O
LEU
B
180
77.763
38.429
−32.172
1.00
35.92
O


ATOM
11374
N
ALA
B
181
79.421
37.605
−33.458
1.00
34.89
N


ATOM
11375
CA
ALA
B
181
78.521
37.071
−34.489
1.00
33.89
C


ATOM
11377
CB
ALA
B
181
79.319
36.544
−35.681
1.00
33.20
C


ATOM
11381
C
ALA
B
181
77.616
35.972
−33.932
1.00
33.29
C


ATOM
11382
O
ALA
B
181
76.416
35.970
−34.192
1.00
33.41
O


ATOM
11384
N
GLU
B
182
78.193
35.044
−33.168
1.00
32.95
N


ATOM
11385
CA
GLU
B
182
77.415
33.959
−32.554
1.00
32.47
C


ATOM
11387
CB
GLU
B
182
78.330
32.913
−31.903
1.00
33.23
C


ATOM
11390
CG
GLU
B
182
78.903
31.901
−32.900
1.00
38.76
C


ATOM
11393
CD
GLU
B
182
80.089
31.104
−32.358
1.00
46.12
C


ATOM
11394
OE1
GLU
B
182
80.432
31.251
−31.161
1.00
53.48
O


ATOM
11395
OE2
GLU
B
182
80.683
30.326
−33.140
1.00
48.05
O


ATOM
11396
C
GLU
B
182
76.411
34.497
−31.539
1.00
30.26
C


ATOM
11397
O
GLU
B
182
75.285
34.011
−31.474
1.00
30.91
O


ATOM
11399
N
GLN
B
183
76.820
35.504
−30.766
1.00
27.88
N


ATOM
11400
CA
GLN
B
183
75.934
36.159
−29.797
1.00
26.22
C


ATOM
11402
CB
GLN
B
183
76.662
37.284
−29.042
1.00
26.65
C


ATOM
11405
CG
GLN
B
183
77.538
36.829
−27.875
1.00
28.31
C


ATOM
11408
CD
GLN
B
183
76.759
36.141
−26.769
1.00
30.87
C


ATOM
11409
OE1
GLN
B
183
75.557
36.349
−26.610
1.00
34.80
O


ATOM
11410
NE2
GLN
B
183
77.445
35.311
−25.999
1.00
33.35
N


ATOM
11413
C
GLN
B
183
74.694
36.739
−30.467
1.00
24.60
C


ATOM
11414
O
GLN
B
183
73.576
36.553
−29.972
1.00
23.70
O


ATOM
11416
N
VAL
B
184
74.905
37.443
−31.582
1.00
23.09
N


ATOM
11417
CA
VAL
B
184
73.813
38.062
−32.349
1.00
21.38
C


ATOM
11419
CB
VAL
B
184
74.345
38.953
−33.508
1.00
21.70
C


ATOM
11421
CG1
VAL
B
184
73.195
39.445
−34.392
1.00
19.46
C


ATOM
11425
CG2
VAL
B
184
75.147
40.132
−32.962
1.00
20.43
C


ATOM
11429
C
VAL
B
184
72.877
37.002
−32.924
1.00
20.07
C


ATOM
11430
O
VAL
B
184
71.661
37.092
−32.765
1.00
19.93
O


ATOM
11432
N
ASN
B
185
73.446
36.000
−33.585
1.00
19.38
N


ATOM
11433
CA
ASN
B
185
72.656
34.900
−34.139
1.00
19.54
C


ATOM
11435
CB
ASN
B
185
73.556
33.865
−34.824
1.00
19.91
C


ATOM
11438
CG
ASN
B
185
74.126
34.365
−36.148
1.00
21.54
C


ATOM
11439
OD1
ASN
B
185
73.403
34.916
−36.980
1.00
22.70
O


ATOM
11440
ND2
ASN
B
185
75.429
34.163
−36.349
1.00
21.22
N


ATOM
11443
C
ASN
B
185
71.822
34.226
−33.064
1.00
18.96
C


ATOM
11444
O
ASN
B
185
70.635
33.962
−33.261
1.00
19.39
O


ATOM
11446
N
HIS
B
186
72.453
33.970
−31.922
1.00
18.64
N


ATOM
11447
CA
HIS
B
186
71.778
33.393
−30.761
1.00
18.00
C


ATOM
11449
CB
HIS
B
186
72.771
33.265
−29.606
1.00
18.39
C


ATOM
11452
CG
HIS
B
186
72.250
32.500
−28.428
1.00
20.53
C


ATOM
11453
ND1
HIS
B
186
72.158
31.124
−28.414
1.00
23.41
N


ATOM
11455
CE1
HIS
B
186
71.689
30.728
−27.244
1.00
23.43
C


ATOM
11457
NE2
HIS
B
186
71.481
31.796
−26.496
1.00
22.88
N


ATOM
11459
CD2
HIS
B
186
71.829
32.917
−27.211
1.00
21.33
C


ATOM
11461
C
HIS
B
186
70.581
34.244
−30.340
1.00
16.85
C


ATOM
11462
O
HIS
B
186
69.492
33.718
−30.094
1.00
16.23
O


ATOM
11464
N
ALA
B
187
70.788
35.559
−30.274
1.00
16.03
N


ATOM
11465
CA
ALA
B
187
69.738
36.496
−29.870
1.00
15.40
C


ATOM
11467
CB
ALA
B
187
70.308
37.884
−29.674
1.00
13.81
C


ATOM
11471
C
ALA
B
187
68.583
36.534
−30.866
1.00
16.36
C


ATOM
11472
O
ALA
B
187
67.422
36.515
−30.459
1.00
16.33
O


ATOM
11474
N
LEU
B
188
68.898
36.579
−32.162
1.00
17.87
N


ATOM
11475
CA
LEU
B
188
67.862
36.576
−33.209
1.00
18.60
C


ATOM
11477
CB
LEU
B
188
68.448
36.922
−34.579
1.00
18.53
C


ATOM
11480
CG
LEU
B
188
69.069
38.309
−34.731
1.00
20.29
C


ATOM
11482
CD1
LEU
B
188
69.627
38.474
−36.145
1.00
15.82
C


ATOM
11486
CD2
LEU
B
188
68.066
39.408
−34.401
1.00
18.27
C


ATOM
11490
C
LEU
B
188
67.123
35.242
−33.302
1.00
19.33
C


ATOM
11491
O
LEU
B
188
65.937
35.222
−33.619
1.00
19.98
O


ATOM
11493
N
GLU
B
189
67.817
34.133
−33.041
1.00
19.94
N


ATOM
11494
CA
GLU
B
189
67.163
32.820
−32.974
1.00
21.03
C


ATOM
11496
CB
GLU
B
189
68.180
31.732
−32.612
1.00
21.46
C


ATOM
11499
CG
GLU
B
189
67.636
30.301
−32.648
1.00
26.25
C


ATOM
11502
CD
GLU
B
189
68.581
29.294
−32.010
1.00
31.11
C


ATOM
11503
OE1
GLU
B
189
68.956
29.496
−30.840
1.00
36.55
O


ATOM
11504
OE2
GLU
B
189
68.938
28.294
−32.666
1.00
34.01
O


ATOM
11505
C
GLU
B
189
66.022
32.844
−31.945
1.00
21.47
C


ATOM
11506
O
GLU
B
189
64.921
32.369
−32.210
1.00
21.87
O


ATOM
11508
N
LEU
B
190
66.307
33.408
−30.777
1.00
21.59
N


ATOM
11509
CA
LEU
B
190
65.357
33.505
−29.679
1.00
21.97
C


ATOM
11511
CB
LEU
B
190
65.006
32.114
−29.131
1.00
22.14
C


ATOM
11514
CG
LEU
B
190
63.792
32.034
−28.196
1.00
25.30
C


ATOM
11516
CD1
LEU
B
190
62.506
32.358
−28.950
1.00
19.09
C


ATOM
11520
CD2
LEU
B
190
63.693
30.662
−27.517
1.00
22.75
C


ATOM
11524
C
LEU
B
190
66.034
34.341
−28.590
1.00
22.26
C


ATOM
11525
O
LEU
B
190
67.231
34.160
−28.336
1.00
22.65
O


ATOM
11527
N
PRO
B
191
65.295
35.269
−27.958
1.00
21.56
N


ATOM
11528
CA
PRO
B
191
65.899
36.089
−26.917
1.00
20.80
C


ATOM
11530
CB
PRO
B
191
65.000
37.316
−26.890
1.00
21.08
C


ATOM
11533
CG
PRO
B
191
63.662
36.791
−27.242
1.00
21.92
C


ATOM
11536
CD
PRO
B
191
63.896
35.657
−28.212
1.00
22.13
C


ATOM
11539
C
PRO
B
191
65.885
35.390
−25.562
1.00
21.09
C


ATOM
11540
O
PRO
B
191
65.105
34.455
−25.361
1.00
21.24
O


ATOM
11541
N
LEU
B
192
66.731
35.866
−24.647
1.00
20.11
N


ATOM
11542
CA
LEU
B
192
66.899
35.267
−23.323
1.00
19.67
C


ATOM
11544
CB
LEU
B
192
67.835
36.128
−22.472
1.00
20.37
C


ATOM
11547
CG
LEU
B
192
69.299
36.212
−22.923
1.00
23.99
C


ATOM
11549
CD1
LEU
B
192
70.032
37.371
−22.243
1.00
24.02
C


ATOM
11553
CD2
LEU
B
192
70.011
34.894
−22.663
1.00
23.49
C


ATOM
11557
C
LEU
B
192
65.583
35.085
−22.576
1.00
19.31
C


ATOM
11558
O
LEU
B
192
65.322
34.011
−22.019
1.00
20.25
O


ATOM
11560
N
HIS
B
193
64.755
36.127
−22.577
1.00
18.28
N


ATOM
11561
CA
HIS
B
193
63.482
36.110
−21.858
1.00
18.62
C


ATOM
11563
CB
HIS
B
193
62.751
37.450
−21.992
1.00
18.42
C


ATOM
11566
CG
HIS
B
193
61.609
37.616
−21.038
1.00
18.33
C


ATOM
11567
ND1
HIS
B
193
61.765
37.534
−19.671
1.00
18.12
N


ATOM
11569
CE1
HIS
B
193
60.596
37.734
−19.083
1.00
17.54
C


ATOM
11571
NE2
HIS
B
193
59.688
37.944
−20.018
1.00
17.57
N


ATOM
11573
CD2
HIS
B
193
60.296
37.878
−21.251
1.00
22.36
C


ATOM
11575
C
HIS
B
193
62.554
34.988
−22.302
1.00
19.69
C


ATOM
11576
O
HIS
B
193
61.725
34.536
−21.515
1.00
20.11
O


ATOM
11578
N
ARG
B
194
62.685
34.537
−23.548
1.00
20.56
N


ATOM
11579
CA
ARG
B
194
61.864
33.437
−24.046
1.00
22.15
C


ATOM
11581
CB
ARG
B
194
61.352
33.753
−25.445
1.00
22.16
C


ATOM
11584
CG
ARG
B
194
60.462
34.975
−25.484
1.00
23.96
C


ATOM
11587
CD
ARG
B
194
59.755
35.109
−26.811
1.00
25.22
C


ATOM
11590
NE
ARG
B
194
58.896
36.290
−26.833
1.00
26.33
N


ATOM
11592
CZ
ARG
B
194
58.332
36.806
−27.925
1.00
24.20
C


ATOM
11593
NH1
ARG
B
194
58.518
36.256
−29.121
1.00
25.70
N


ATOM
11596
NH2
ARG
B
194
57.576
37.888
−27.816
1.00
23.60
N


ATOM
11599
C
ARG
B
194
62.570
32.075
−24.053
1.00
23.48
C


ATOM
11600
O
ARG
B
194
61.904
31.053
−24.189
1.00
25.36
O


ATOM
11602
N
ARG
B
195
63.897
32.050
−23.902
1.00
22.67
N


ATOM
11603
CA
ARG
B
195
64.654
30.794
−23.936
1.00
21.30
C


ATOM
11605
CB
ARG
B
195
66.105
31.079
−24.302
1.00
21.39
C


ATOM
11608
CG
ARG
B
195
66.886
29.867
−24.742
1.00
21.47
C


ATOM
11611
CD
ARG
B
195
68.323
30.241
−25.060
1.00
21.44
C


ATOM
11614
NE
ARG
B
195
68.432
31.224
−26.141
1.00
20.11
N


ATOM
11616
CZ
ARG
B
195
68.427
30.936
−27.446
1.00
22.99
C


ATOM
11617
NH1
ARG
B
195
68.311
29.679
−27.883
1.00
21.23
N


ATOM
11620
NH2
ARG
B
195
68.540
31.920
−28.332
1.00
26.18
N


ATOM
11623
C
ARG
B
195
64.602
30.095
−22.590
1.00
20.50
C


ATOM
11624
O
ARG
B
195
64.617
30.752
−21.554
1.00
21.55
O


ATOM
11626
N
THR
B
196
64.552
28.767
−22.593
1.00
20.35
N


ATOM
11627
CA
THR
B
196
64.555
28.011
−21.328
1.00
19.52
C


ATOM
11629
CB
THR
B
196
64.182
26.502
−21.498
1.00
19.58
C


ATOM
11631
OG1
THR
B
196
65.060
25.872
−22.438
1.00
17.84
O


ATOM
11633
CG2
THR
B
196
62.736
26.346
−21.953
1.00
15.41
C


ATOM
11637
C
THR
B
196
65.916
28.128
−20.645
1.00
18.43
C


ATOM
11638
O
THR
B
196
66.919
28.415
−21.293
1.00
18.12
O


ATOM
11640
N
GLN
B
197
65.926
27.899
−19.335
1.00
17.17
N


ATOM
11641
CA
GLN
B
197
67.087
28.172
−18.501
1.00
17.20
C


ATOM
11643
CB
GLN
B
197
66.690
28.085
−17.018
1.00
17.39
C


ATOM
11646
CG
GLN
B
197
67.515
28.969
−16.072
1.00
19.60
C


ATOM
11649
CD
GLN
B
197
68.758
28.289
−15.508
1.00
22.54
C


ATOM
11650
OE1
GLN
B
197
69.183
27.229
−15.978
1.00
22.58
O


ATOM
11651
NE2
GLN
B
197
69.346
28.906
−14.487
1.00
22.18
N


ATOM
11654
C
GLN
B
197
68.285
27.248
−18.803
1.00
17.52
C


ATOM
11655
O
GLN
B
197
69.419
27.727
−18.906
1.00
18.43
O


ATOM
11657
N
ARG
B
198
68.041
25.943
−18.940
1.00
16.18
N


ATOM
11658
CA
ARG
B
198
69.120
24.981
−19.194
1.00
16.69
C


ATOM
11660
CB
ARG
B
198
68.641
23.538
−19.005
1.00
15.67
C


ATOM
11663
CG
ARG
B
198
68.201
23.183
−17.597
1.00
15.18
C


ATOM
11666
CD
ARG
B
198
69.376
23.008
−16.639
1.00
16.81
C


ATOM
11669
NE
ARG
B
198
69.913
24.279
−16.153
1.00
18.44
N


ATOM
11671
CZ
ARG
B
198
70.934
24.393
−15.302
1.00
17.91
C


ATOM
11672
NH1
ARG
B
198
71.546
23.312
−14.820
1.00
17.59
N


ATOM
11675
NH2
ARG
B
198
71.339
25.597
−14.920
1.00
17.06
N


ATOM
11678
C
ARG
B
198
69.710
25.141
−20.595
1.00
18.02
C


ATOM
11679
O
ARG
B
198
70.927
25.033
−20.780
1.00
18.32
O


ATOM
11681
N
LEU
B
199
68.856
25.391
−21.584
1.00
18.62
N


ATOM
11682
CA
LEU
B
199
69.338
25.599
−22.942
1.00
19.22
C


ATOM
11684
CB
LEU
B
199
68.191
25.787
−23.928
1.00
20.05
C


ATOM
11687
CG
LEU
B
199
67.554
24.491
−24.421
1.00
23.38
C


ATOM
11689
CD1
LEU
B
199
66.370
24.822
−25.325
1.00
24.54
C


ATOM
11693
CD2
LEU
B
199
68.588
23.616
−25.139
1.00
22.02
C


ATOM
11697
C
LEU
B
199
70.250
26.802
−23.005
1.00
19.41
C


ATOM
11698
O
LEU
B
199
71.288
26.751
−23.658
1.00
20.28
O


ATOM
11700
N
GLU
B
200
69.868
27.885
−22.333
1.00
19.03
N


ATOM
11701
CA
GLU
B
200
70.716
29.068
−22.296
1.00
19.14
C


ATOM
11703
CB
GLU
B
200
70.025
30.235
−21.592
1.00
18.98
C


ATOM
11706
CG
GLU
B
200
70.877
31.492
−21.484
1.00
17.68
C


ATOM
11709
CD
GLU
B
200
71.430
31.959
−22.816
1.00
23.55
C


ATOM
11710
OE1
GLU
B
200
70.754
31.793
−23.847
1.00
25.99
O


ATOM
11711
OE2
GLU
B
200
72.543
32.516
−22.837
1.00
29.25
O


ATOM
11712
C
GLU
B
200
72.029
28.753
−21.603
1.00
19.49
C


ATOM
11713
O
GLU
B
200
73.086
29.187
−22.060
1.00
20.17
O


ATOM
11715
N
ALA
B
201
71.958
27.998
−20.509
1.00
18.86
N


ATOM
11716
CA
ALA
B
201
73.153
27.617
−19.766
1.00
19.01
C


ATOM
11718
CB
ALA
B
201
72.782
26.801
−18.536
1.00
18.47
C


ATOM
11722
C
ALA
B
201
74.109
26.835
−20.656
1.00
19.34
C


ATOM
11723
O
ALA
B
201
75.266
27.234
−20.838
1.00
20.50
O


ATOM
11725
N
VAL
B
202
73.614
25.742
−21.237
1.00
18.86
N


ATOM
11726
CA
VAL
B
202
74.455
24.855
−22.044
1.00
18.25
C


ATOM
11728
CB
VAL
B
202
73.665
23.622
−22.586
1.00
18.35
C


ATOM
11730
CG1
VAL
B
202
72.750
24.015
−23.711
1.00
16.77
C


ATOM
11734
CG2
VAL
B
202
74.622
22.531
−23.048
1.00
16.57
C


ATOM
11738
C
VAL
B
202
75.121
25.618
−23.185
1.00
17.55
C


ATOM
11739
O
VAL
B
202
76.262
25.334
−23.543
1.00
18.86
O


ATOM
11741
N
TRP
B
203
74.417
26.598
−23.740
1.00
16.56
N


ATOM
11742
CA
TRP
B
203
74.976
27.421
−24.799
1.00
16.59
C


ATOM
11744
CB
TRP
B
203
73.873
28.155
−25.558
1.00
16.92
C


ATOM
11747
CG
TRP
B
203
74.399
28.864
−26.754
1.00
18.88
C


ATOM
11748
CD1
TRP
B
203
74.532
28.364
−28.019
1.00
17.65
C


ATOM
11750
NE1
TRP
B
203
75.090
29.309
−28.842
1.00
15.37
N


ATOM
11752
CE2
TRP
B
203
75.328
30.445
−28.114
1.00
16.30
C


ATOM
11753
CD2
TRP
B
203
74.907
30.197
−26.792
1.00
18.21
C


ATOM
11754
CE3
TRP
B
203
75.048
31.205
−25.837
1.00
17.36
C


ATOM
11756
CZ3
TRP
B
203
75.591
32.410
−26.224
1.00
20.82
C


ATOM
11758
CH2
TRP
B
203
75.997
32.632
−27.549
1.00
20.62
C


ATOM
11760
CZ2
TRP
B
203
75.872
31.662
−28.506
1.00
19.32
C


ATOM
11762
C
TRP
B
203
75.994
28.424
−24.250
1.00
16.78
C


ATOM
11763
O
TRP
B
203
77.085
28.565
−24.808
1.00
16.80
O


ATOM
11765
N
SER
B
204
75.643
29.115
−23.165
1.00
16.20
N


ATOM
11766
CA
SER
B
204
76.510
30.159
−22.615
1.00
15.80
C


ATOM
11768
CB
SER
B
204
75.758
31.040
−21.623
1.00
15.90
C


ATOM
11771
OG
SER
B
204
75.049
32.049
−22.314
1.00
18.88
O


ATOM
11773
C
SER
B
204
77.782
29.613
−21.973
1.00
15.47
C


ATOM
11774
O
SER
B
204
78.836
30.239
−22.075
1.00
15.64
O


ATOM
11776
N
ILE
B
205
77.700
28.457
−21.320
1.00
14.25
N


ATOM
11777
CA
ILE
B
205
78.903
27.830
−20.785
1.00
13.83
C


ATOM
11779
CB
ILE
B
205
78.599
26.525
−20.013
1.00
14.16
C


ATOM
11781
CG1
ILE
B
205
77.851
26.854
−18.711
1.00
13.77
C


ATOM
11784
CD1
ILE
B
205
77.428
25.655
−17.887
1.00
9.36
C


ATOM
11788
CG2
ILE
B
205
79.893
25.778
−19.710
1.00
11.61
C


ATOM
11792
C
ILE
B
205
79.915
27.576
−21.907
1.00
14.14
C


ATOM
11793
O
ILE
B
205
81.103
27.832
−21.739
1.00
13.11
O


ATOM
11795
N
GLU
B
206
79.438
27.110
−23.060
1.00
15.22
N


ATOM
11796
CA
GLU
B
206
80.317
26.851
−24.209
1.00
15.60
C


ATOM
11798
CB
GLU
B
206
79.560
26.067
−25.289
1.00
16.51
C


ATOM
11801
CG
GLU
B
206
80.357
25.729
−26.563
1.00
19.18
C


ATOM
11804
CD
GLU
B
206
81.507
24.764
−26.334
1.00
22.94
C


ATOM
11805
OE1
GLU
B
206
81.580
24.144
−25.248
1.00
27.63
O


ATOM
11806
OE2
GLU
B
206
82.345
24.627
−27.254
1.00
22.01
O


ATOM
11807
C
GLU
B
206
80.921
28.138
−24.794
1.00
14.48
C


ATOM
11808
O
GLU
B
206
82.082
28.147
−25.194
1.00
14.32
O


ATOM
11810
N
ALA
B
207
80.135
29.212
−24.837
1.00
14.37
N


ATOM
11811
CA
ALA
B
207
80.599
30.505
−25.365
1.00
14.47
C


ATOM
11813
CB
ALA
B
207
79.417
31.407
−25.656
1.00
13.19
C


ATOM
11817
C
ALA
B
207
81.570
31.205
−24.407
1.00
15.42
C


ATOM
11818
O
ALA
B
207
82.619
31.719
−24.823
1.00
14.00
O


ATOM
11820
N
TYR
B
208
81.208
31.217
−23.126
1.00
16.83
N


ATOM
11821
CA
TYR
B
208
82.036
31.822
−22.085
1.00
17.39
C


ATOM
11823
CB
TYR
B
208
81.295
31.775
−20.748
1.00
16.80
C


ATOM
11826
CG
TYR
B
208
81.878
32.675
−19.681
1.00
19.11
C


ATOM
11827
CD1
TYR
B
208
81.906
34.059
−19.849
1.00
20.27
C


ATOM
11829
CE1
TYR
B
208
82.435
34.893
−18.870
1.00
21.22
C


ATOM
11831
CZ
TYR
B
208
82.939
34.342
−17.708
1.00
21.24
C


ATOM
11832
OH
TYR
B
208
83.460
35.154
−16.739
1.00
25.72
O


ATOM
11834
CE2
TYR
B
208
82.925
32.974
−17.514
1.00
21.97
C


ATOM
11836
CD2
TYR
B
208
82.393
32.146
−18.496
1.00
20.32
C


ATOM
11838
C
TYR
B
208
83.387
31.109
−21.970
1.00
18.26
C


ATOM
11839
O
TYR
B
208
84.420
31.740
−21.752
1.00
17.72
O


ATOM
11841
N
ARG
B
209
83.361
29.788
−22.123
1.00
19.60
N


ATOM
11842
CA
ARG
B
209
84.554
28.948
−22.032
1.00
20.63
C


ATOM
11844
CB
ARG
B
209
84.149
27.488
−22.263
1.00
20.66
C


ATOM
11847
CG
ARG
B
209
85.258
26.471
−22.151
1.00
23.66
C


ATOM
11850
CD
ARG
B
209
84.896
25.207
−22.904
1.00
24.35
C


ATOM
11853
NE
ARG
B
209
83.848
24.450
−22.231
1.00
21.90
N


ATOM
11855
CZ
ARG
B
209
84.045
23.557
−21.259
1.00
21.36
C


ATOM
11856
NH1
ARG
B
209
85.265
23.282
−20.795
1.00
16.58
N


ATOM
11859
NH2
ARG
B
209
82.998
22.929
−20.743
1.00
26.47
N


ATOM
11862
C
ARG
B
209
85.631
29.362
−23.038
1.00
21.82
C


ATOM
11863
O
ARG
B
209
86.825
29.247
−22.756
1.00
22.67
O


ATOM
11865
N
LYS
B
210
85.208
29.845
−24.204
1.00
22.93
N


ATOM
11866
CA
LYS
B
210
86.138
30.254
−25.263
1.00
23.88
C


ATOM
11868
CB
LYS
B
210
85.450
30.192
−26.631
1.00
23.97
C


ATOM
11871
CG
LYS
B
210
84.944
28.810
−27.024
1.00
24.44
C


ATOM
11874
CD
LYS
B
210
83.911
28.911
−28.133
1.00
27.36
C


ATOM
11877
CE
LYS
B
210
83.281
27.570
−28.444
1.00
28.72
C


ATOM
11880
NZ
LYS
B
210
82.157
27.723
−29.405
1.00
27.56
N


ATOM
11884
C
LYS
B
210
86.732
31.652
−25.060
1.00
25.02
C


ATOM
11885
O
LYS
B
210
87.806
31.935
−25.585
1.00
25.55
O


ATOM
11887
N
LYS
B
211
86.042
32.523
−24.320
1.00
26.93
N


ATOM
11888
CA
LYS
B
211
86.548
33.875
−24.042
1.00
28.85
C


ATOM
11890
CB
LYS
B
211
85.579
34.667
−23.155
1.00
29.58
C


ATOM
11893
CG
LYS
B
211
84.262
35.079
−23.817
1.00
32.78
C


ATOM
11896
CD
LYS
B
211
83.566
36.177
−23.005
1.00
37.23
C


ATOM
11899
CE
LYS
B
211
82.125
36.395
−23.454
1.00
40.81
C


ATOM
11902
NZ
LYS
B
211
82.027
36.756
−24.893
1.00
43.15
N


ATOM
11906
C
LYS
B
211
87.918
33.816
−23.356
1.00
30.15
C


ATOM
11907
O
LYS
B
211
88.113
33.043
−22.411
1.00
30.30
O


ATOM
11909
N
GLU
B
212
88.859
34.628
−23.838
1.00
30.90
N


ATOM
11910
CA
GLU
B
212
90.217
34.637
−23.292
1.00
31.34
C


ATOM
11912
CB
GLU
B
212
91.193
35.334
−24.250
1.00
32.12
C


ATOM
11915
CG
GLU
B
212
92.669
35.267
−23.794
1.00
35.82
C


ATOM
11918
CD
GLU
B
212
93.661
35.001
−24.929
1.00
39.82
C


ATOM
11919
OE1
GLU
B
212
93.233
34.620
−26.041
1.00
42.39
O


ATOM
11920
OE2
GLU
B
212
94.881
35.156
−24.699
1.00
39.88
O


ATOM
11921
C
GLU
B
212
90.267
35.286
−21.909
1.00
30.16
C


ATOM
11922
O
GLU
B
212
91.099
34.917
−21.085
1.00
30.48
O


ATOM
11924
N
ASP
B
213
89.374
36.238
−21.655
1.00
28.63
N


ATOM
11925
CA
ASP
B
213
89.293
36.890
−20.344
1.00
27.81
C


ATOM
11927
CB
ASP
B
213
89.228
38.419
−20.504
1.00
28.13
C


ATOM
11930
CG
ASP
B
213
88.096
38.880
−21.418
1.00
31.91
C


ATOM
11931
OD1
ASP
B
213
87.454
38.028
−22.078
1.00
35.70
O


ATOM
11932
OD2
ASP
B
213
87.853
40.105
−21.479
1.00
36.60
O


ATOM
11933
C
ASP
B
213
88.111
36.363
−19.512
1.00
26.08
C


ATOM
11934
O
ASP
B
213
87.454
37.120
−18.797
1.00
26.21
O


ATOM
11936
N
ALA
B
214
87.854
35.059
−19.598
1.00
23.51
N


ATOM
11937
CA
ALA
B
214
86.750
34.442
−18.866
1.00
21.13
C


ATOM
11939
CB
ALA
B
214
86.362
33.133
−19.511
1.00
21.07
C


ATOM
11943
C
ALA
B
214
87.158
34.208
−17.419
1.00
19.11
C


ATOM
11944
O
ALA
B
214
88.244
33.692
−17.166
1.00
18.32
O


ATOM
11946
N
ASN
B
215
86.293
34.595
−16.479
1.00
17.00
N


ATOM
11947
CA
ASN
B
215
86.528
34.338
−15.060
1.00
15.97
C


ATOM
11949
CB
ASN
B
215
85.507
35.084
−14.189
1.00
15.62
C


ATOM
11952
CG
ASN
B
215
85.790
34.945
−12.690
1.00
16.18
C


ATOM
11953
OD1
ASN
B
215
85.766
33.841
−12.134
1.00
12.71
O


ATOM
11954
ND2
ASN
B
215
86.049
36.071
−12.031
1.00
17.08
N


ATOM
11957
C
ASN
B
215
86.480
32.830
−14.791
1.00
15.80
C


ATOM
11958
O
ASN
B
215
85.444
32.187
−14.964
1.00
15.66
O


ATOM
11960
N
GLN
B
216
87.609
32.275
−14.368
1.00
15.71
N


ATOM
11961
CA
GLN
B
216
87.751
30.832
−14.231
1.00
16.29
C


ATOM
11963
CB
GLN
B
216
89.220
30.461
−14.040
1.00
16.24
C


ATOM
11966
CG
GLN
B
216
90.104
30.863
−15.220
1.00
19.78
C


ATOM
11969
CD
GLN
B
216
89.645
30.249
−16.534
1.00
23.16
C


ATOM
11970
OE1
GLN
B
216
90.015
29.123
−16.871
1.00
25.74
O


ATOM
11971
NE2
GLN
B
216
88.837
30.993
−17.286
1.00
23.59
N


ATOM
11974
C
GLN
B
216
86.902
30.273
−13.090
1.00
16.43
C


ATOM
11975
O
GLN
B
216
86.283
29.211
−13.238
1.00
16.99
O


ATOM
11977
N
VAL
B
217
86.857
30.991
−11.971
1.00
15.28
N


ATOM
11978
CA
VAL
B
217
86.033
30.579
−10.838
1.00
14.30
C


ATOM
11980
CB
VAL
B
217
86.155
31.559
−9.654
1.00
14.53
C


ATOM
11982
CG1
VAL
B
217
85.180
31.169
−8.536
1.00
13.56
C


ATOM
11986
CG2
VAL
B
217
87.591
31.607
−9.144
1.00
9.92
C


ATOM
11990
C
VAL
B
217
84.563
30.473
−11.256
1.00
13.94
C


ATOM
11991
O
VAL
B
217
83.919
29.447
−11.051
1.00
14.19
O


ATOM
11993
N
LEU
B
218
84.049
31.539
−11.857
1.00
13.24
N


ATOM
11994
CA
LEU
B
218
82.668
31.573
−12.303
1.00
12.51
C


ATOM
11996
CB
LEU
B
218
82.345
32.938
−12.904
1.00
11.55
C


ATOM
11999
CG
LEU
B
218
80.940
33.178
−13.471
1.00
12.38
C


ATOM
12001
CD1
LEU
B
218
79.829
32.666
−12.538
1.00
6.13
C


ATOM
12005
CD2
LEU
B
218
80.771
34.672
−13.777
1.00
5.08
C


ATOM
12009
C
LEU
B
218
82.398
30.464
−13.316
1.00
13.27
C


ATOM
12010
O
LEU
B
218
81.343
29.819
−13.262
1.00
13.54
O


ATOM
12012
N
LEU
B
219
83.357
30.245
−14.221
1.00
13.34
N


ATOM
12013
CA
LEU
B
219
83.257
29.209
−15.254
1.00
12.59
C


ATOM
12015
CB
LEU
B
219
84.433
29.301
−16.223
1.00
12.96
C


ATOM
12018
CG
LEU
B
219
84.587
28.183
−17.265
1.00
12.77
C


ATOM
12020
CD1
LEU
B
219
83.293
27.926
−18.024
1.00
5.60
C


ATOM
12024
CD2
LEU
B
219
85.725
28.533
−18.224
1.00
10.15
C


ATOM
12028
C
LEU
B
219
83.225
27.818
−14.659
1.00
12.92
C


ATOM
12029
O
LEU
B
219
82.365
27.005
−15.008
1.00
13.24
O


ATOM
12031
N
GLU
B
220
84.174
27.531
−13.775
1.00
12.89
N


ATOM
12032
CA
GLU
B
220
84.237
26.211
−13.172
1.00
13.07
C


ATOM
12034
CB
GLU
B
220
85.442
26.074
−12.258
1.00
13.54
C


ATOM
12037
CG
GLU
B
220
85.602
24.663
−11.719
1.00
14.51
C


ATOM
12040
CD
GLU
B
220
86.971
24.382
−11.155
1.00
13.18
C


ATOM
12041
OE1
GLU
B
220
87.748
25.338
−10.917
1.00
11.03
O


ATOM
12042
OE2
GLU
B
220
87.258
23.184
−10.952
1.00
15.01
O


ATOM
12043
C
GLU
B
220
82.973
25.884
−12.394
1.00
13.97
C


ATOM
12044
O
GLU
B
220
82.505
24.754
−12.438
1.00
15.47
O


ATOM
12046
N
LEU
B
221
82.423
26.865
−11.682
1.00
13.71
N


ATOM
12047
CA
LEU
B
221
81.206
26.645
−10.911
1.00
13.62
C


ATOM
12049
CB
LEU
B
221
80.883
27.874
−10.056
1.00
13.55
C


ATOM
12052
CG
LEU
B
221
79.673
27.780
−9.112
1.00
13.36
C


ATOM
12054
CD1
LEU
B
221
79.907
26.790
−7.974
1.00
5.36
C


ATOM
12058
CD2
LEU
B
221
79.341
29.163
−8.561
1.00
11.48
C


ATOM
12062
C
LEU
B
221
80.046
26.313
−11.850
1.00
14.45
C


ATOM
12063
O
LEU
B
221
79.260
25.407
−11.576
1.00
15.34
O


ATOM
12065
N
ALA
B
222
79.963
27.038
−12.964
1.00
14.56
N


ATOM
12066
CA
ALA
B
222
78.929
26.822
−13.979
1.00
14.23
C


ATOM
12068
CB
ALA
B
222
79.114
27.809
−15.126
1.00
13.99
C


ATOM
12072
C
ALA
B
222
78.919
25.391
−14.513
1.00
14.13
C


ATOM
12073
O
ALA
B
222
77.869
24.770
−14.605
1.00
13.50
O


ATOM
12075
N
ILE
B
223
80.093
24.871
−14.856
1.00
15.00
N


ATOM
12076
CA
ILE
B
223
80.209
23.498
−15.356
1.00
15.35
C


ATOM
12078
CB
ILE
B
223
81.631
23.181
−15.854
1.00
15.31
C


ATOM
12080
CG1
ILE
B
223
82.022
24.094
−17.018
1.00
15.24
C


ATOM
12083
CD1
ILE
B
223
83.500
24.052
−17.330
1.00
11.25
C


ATOM
12087
CG2
ILE
B
223
81.730
21.720
−16.296
1.00
13.41
C


ATOM
12091
C
ILE
B
223
79.881
22.498
−14.252
1.00
15.97
C


ATOM
12092
O
ILE
B
223
79.180
21.498
−14.477
1.00
16.87
O


ATOM
12094
N
LEU
B
224
80.393
22.781
−13.061
1.00
15.24
N


ATOM
12095
CA
LEU
B
224
80.202
21.914
−11.905
1.00
16.03
C


ATOM
12097
CB
LEU
B
224
81.012
22.450
−10.720
1.00
16.87
C


ATOM
12100
CG
LEU
B
224
81.398
21.486
−9.604
1.00
19.31
C


ATOM
12102
CD1
LEU
B
224
82.677
21.947
−8.915
1.00
18.36
C


ATOM
12106
CD2
LEU
B
224
80.252
21.354
−8.602
1.00
22.58
C


ATOM
12110
C
LEU
B
224
78.718
21.810
−11.558
1.00
16.27
C


ATOM
12111
O
LEU
B
224
78.178
20.709
−11.448
1.00
17.89
O


ATOM
12113
N
ASP
B
225
78.053
22.957
−11.432
1.00
15.39
N


ATOM
12114
CA
ASP
B
225
76.632
22.994
−11.082
1.00
14.07
C


ATOM
12116
CB
ASP
B
225
76.175
24.445
−10.849
1.00
13.66
C


ATOM
12119
CG
ASP
B
225
74.860
24.540
−10.078
1.00
11.65
C


ATOM
12120
OD1
ASP
B
225
74.844
24.220
−8.879
1.00
13.94
O


ATOM
12121
OD2
ASP
B
225
73.841
24.951
−10.664
1.00
12.79
O


ATOM
12122
C
ASP
B
225
75.771
22.327
−12.159
1.00
14.25
C


ATOM
12123
O
ASP
B
225
74.913
21.513
−11.846
1.00
15.30
O


ATOM
12125
N
TYR
B
226
76.003
22.667
−13.424
1.00
14.55
N


ATOM
12126
CA
TYR
B
226
75.221
22.100
−14.528
1.00
15.33
C


ATOM
12128
CB
TYR
B
226
75.718
22.615
−15.880
1.00
15.41
C


ATOM
12131
CG
TYR
B
226
74.866
22.201
−17.064
1.00
16.23
C


ATOM
12132
CD1
TYR
B
226
75.035
20.957
−17.678
1.00
17.02
C


ATOM
12134
CE1
TYR
B
226
74.255
20.580
−18.768
1.00
15.10
C


ATOM
12136
CZ
TYR
B
226
73.294
21.452
−19.250
1.00
17.43
C


ATOM
12137
OH
TYR
B
226
72.511
21.096
−20.319
1.00
15.92
O


ATOM
12139
CE2
TYR
B
226
73.110
22.686
−18.661
1.00
16.10
C


ATOM
12141
CD2
TYR
B
226
73.892
23.055
−17.577
1.00
16.82
C


ATOM
12143
C
TYR
B
226
75.268
20.577
−14.510
1.00
16.96
C


ATOM
12144
O
TYR
B
226
74.231
19.921
−14.593
1.00
19.18
O


ATOM
12146
N
ASN
B
227
76.467
20.014
−14.395
1.00
17.75
N


ATOM
12147
CA
ASN
B
227
76.614
18.561
−14.371
1.00
17.68
C


ATOM
12149
CB
ASN
B
227
78.086
18.139
−14.488
1.00
16.82
C


ATOM
12152
CG
ASN
B
227
78.632
18.303
−15.900
1.00
14.72
C


ATOM
12153
OD1
ASN
B
227
77.883
18.512
−16.855
1.00
16.14
O


ATOM
12154
ND2
ASN
B
227
79.942
18.209
−16.037
1.00
11.36
N


ATOM
12157
C
ASN
B
227
75.970
17.948
−13.138
1.00
18.57
C


ATOM
12158
O
ASN
B
227
75.389
16.864
−13.220
1.00
19.90
O


ATOM
12160
N
MET
B
228
76.055
18.638
−12.003
1.00
18.84
N


ATOM
12161
CA
MET
B
228
75.392
18.173
−10.793
1.00
19.65
C


ATOM
12163
CB
MET
B
228
75.653
19.126
−9.639
1.00
20.86
C


ATOM
12166
CG
MET
B
228
74.939
18.739
−8.352
1.00
24.77
C


ATOM
12169
SD
MET
B
228
74.627
20.171
−7.322
1.00
34.08
S


ATOM
12170
CE
MET
B
228
73.252
20.947
−8.177
1.00
21.79
C


ATOM
12174
C
MET
B
228
73.886
18.057
−11.029
1.00
20.36
C


ATOM
12175
O
MET
B
228
73.289
17.011
−10.747
1.00
19.80
O


ATOM
12177
N
ILE
B
229
73.283
19.131
−11.549
1.00
19.71
N


ATOM
12178
CA
ILE
B
229
71.856
19.139
−11.852
1.00
19.13
C


ATOM
12180
CB
ILE
B
229
71.360
20.532
−12.330
1.00
19.77
C


ATOM
12182
CG1
ILE
B
229
71.412
21.544
−11.176
1.00
18.86
C


ATOM
12185
CD1
ILE
B
229
71.104
22.968
−11.591
1.00
18.21
C


ATOM
12189
CG2
ILE
B
229
69.936
20.448
−12.883
1.00
14.42
C


ATOM
12193
C
ILE
B
229
71.537
18.080
−12.901
1.00
20.72
C


ATOM
12194
O
ILE
B
229
70.537
17.368
−12.778
1.00
21.03
O


ATOM
12196
N
GLN
B
230
72.389
17.951
−13.918
1.00
21.54
N


ATOM
12197
CA
GLN
B
230
72.172
16.916
−14.928
1.00
21.86
C


ATOM
12199
CB
GLN
B
230
73.239
16.936
−16.026
1.00
21.39
C


ATOM
12202
CG
GLN
B
230
72.760
16.232
−17.284
1.00
22.27
C


ATOM
12205
CD
GLN
B
230
73.845
16.005
−18.316
1.00
26.28
C


ATOM
12206
OE1
GLN
B
230
74.848
15.325
−18.054
1.00
27.63
O


ATOM
12207
NE2
GLN
B
230
73.633
16.540
−19.517
1.00
23.18
N


ATOM
12210
C
GLN
B
230
72.103
15.526
−14.285
1.00
22.16
C


ATOM
12211
O
GLN
B
230
71.338
14.678
−14.725
1.00
23.81
O


ATOM
12213
N
SER
B
231
72.883
15.300
−13.234
1.00
21.57
N


ATOM
12214
CA
SER
B
231
72.842
14.023
−12.537
1.00
21.30
C


ATOM
12216
CB
SER
B
231
73.993
13.908
−11.543
1.00
21.05
C


ATOM
12219
OG
SER
B
231
73.694
14.627
−10.360
1.00
28.21
O


ATOM
12221
C
SER
B
231
71.509
13.810
−11.818
1.00
19.77
C


ATOM
12222
O
SER
B
231
71.044
12.677
−11.707
1.00
20.50
O


ATOM
12224
N
VAL
B
232
70.909
14.888
−11.320
1.00
18.70
N


ATOM
12225
CA
VAL
B
232
69.572
14.818
−10.712
1.00
17.74
C


ATOM
12227
CB
VAL
B
232
69.177
16.144
−10.008
1.00
17.54
C


ATOM
12229
CG1
VAL
B
232
67.734
16.092
−9.533
1.00
16.93
C


ATOM
12233
CG2
VAL
B
232
70.106
16.434
−8.837
1.00
12.46
C


ATOM
12237
C
VAL
B
232
68.507
14.454
−11.756
1.00
17.57
C


ATOM
12238
O
VAL
B
232
67.603
13.671
−11.476
1.00
16.98
O


ATOM
12240
N
TYR
B
233
68.627
15.010
−12.957
1.00
17.65
N


ATOM
12241
CA
TYR
B
233
67.708
14.676
−14.038
1.00
18.54
C


ATOM
12243
CB
TYR
B
233
67.971
15.536
−15.281
1.00
18.25
C


ATOM
12246
CG
TYR
B
233
67.759
17.024
−15.109
1.00
16.48
C


ATOM
12247
CD1
TYR
B
233
66.982
17.532
−14.076
1.00
16.87
C


ATOM
12249
CE1
TYR
B
233
66.791
18.898
−13.927
1.00
19.52
C


ATOM
12251
CZ
TYR
B
233
67.362
19.774
−14.823
1.00
16.87
C


ATOM
12252
OH
TYR
B
233
67.151
21.126
−14.664
1.00
16.45
O


ATOM
12254
CE2
TYR
B
233
68.128
19.295
−15.869
1.00
16.67
C


ATOM
12256
CD2
TYR
B
233
68.320
17.927
−16.008
1.00
16.37
C


ATOM
12258
C
TYR
B
233
67.837
13.203
−14.409
1.00
20.38
C


ATOM
12259
O
TYR
B
233
66.841
12.512
−14.627
1.00
19.61
O


ATOM
12261
N
GLN
B
234
69.077
12.732
−14.484
1.00
22.17
N


ATOM
12262
CA
GLN
B
234
69.344
11.335
−14.790
1.00
23.14
C


ATOM
12264
CB
GLN
B
234
70.850
11.104
−14.985
1.00
22.82
C


ATOM
12267
CG
GLN
B
234
71.334
11.599
−16.355
1.00
25.57
C


ATOM
12270
CD
GLN
B
234
72.841
11.795
−16.451
1.00
29.72
C


ATOM
12271
OE1
GLN
B
234
73.605
11.309
−15.611
1.00
33.47
O


ATOM
12272
NE2
GLN
B
234
73.275
12.512
−17.489
1.00
26.11
N


ATOM
12275
C
GLN
B
234
68.738
10.410
−13.728
1.00
23.92
C


ATOM
12276
O
GLN
B
234
68.224
9.343
−14.063
1.00
24.41
O


ATOM
12278
N
ARG
B
235
68.761
10.825
−12.464
1.00
24.63
N


ATOM
12279
CA
ARG
B
235
68.102
10.053
−11.415
1.00
25.50
C


ATOM
12281
CB
ARG
B
235
68.514
10.523
−10.017
1.00
26.04
C


ATOM
12284
CG
ARG
B
235
67.894
9.695
−8.893
1.00
31.26
C


ATOM
12287
CD
ARG
B
235
68.614
9.854
−7.551
1.00
37.28
C


ATOM
12290
NE
ARG
B
235
68.672
11.251
−7.115
1.00
43.87
N


ATOM
12292
CZ
ARG
B
235
69.755
12.034
−7.154
1.00
48.76
C


ATOM
12293
NH1
ARG
B
235
70.929
11.582
−7.603
1.00
49.24
N


ATOM
12296
NH2
ARG
B
235
69.664
13.291
−6.729
1.00
49.39
N


ATOM
12299
C
ARG
B
235
66.590
10.134
−11.592
1.00
25.89
C


ATOM
12300
O
ARG
B
235
65.905
9.114
−11.507
1.00
26.65
O


ATOM
12302
N
ASP
B
236
66.076
11.335
−11.857
1.00
25.46
N


ATOM
12303
CA
ASP
B
236
64.645
11.517
−12.108
1.00
25.83
C


ATOM
12305
CB
ASP
B
236
64.321
12.983
−12.439
1.00
25.43
C


ATOM
12308
CG
ASP
B
236
64.495
13.925
−11.258
1.00
26.34
C


ATOM
12309
OD1
ASP
B
236
64.577
13.468
−10.094
1.00
25.45
O


ATOM
12310
OD2
ASP
B
236
64.552
15.147
−11.508
1.00
24.68
O


ATOM
12311
C
ASP
B
236
64.160
10.642
−13.270
1.00
26.07
C


ATOM
12312
O
ASP
B
236
63.139
9.964
−13.165
1.00
25.47
O


ATOM
12314
N
LEU
B
237
64.896
10.676
−14.376
1.00
26.92
N


ATOM
12315
CA
LEU
B
237
64.510
9.968
−15.594
1.00
27.78
C


ATOM
12317
CB
LEU
B
237
65.410
10.383
−16.767
1.00
27.40
C


ATOM
12320
CG
LEU
B
237
65.195
9.726
−18.137
1.00
25.46
C


ATOM
12322
CD1
LEU
B
237
63.730
9.638
−18.503
1.00
24.69
C


ATOM
12326
CD2
LEU
B
237
65.949
10.486
−19.212
1.00
23.42
C


ATOM
12330
C
LEU
B
237
64.545
8.453
−15.421
1.00
30.07
C


ATOM
12331
O
LEU
B
237
63.699
7.753
−15.985
1.00
30.83
O


ATOM
12333
N
ARG
B
238
65.516
7.945
−14.660
1.00
31.75
N


ATOM
12334
CA
ARG
B
238
65.608
6.500
−14.406
1.00
32.98
C


ATOM
12336
CB
ARG
B
238
66.862
6.137
−13.592
1.00
33.58
C


ATOM
12339
CG
ARG
B
238
68.159
6.099
−14.397
1.00
36.70
C


ATOM
12342
CD
ARG
B
238
69.265
5.306
−13.681
1.00
40.82
C


ATOM
12345
NE
ARG
B
238
69.652
5.857
−12.371
1.00
44.46
N


ATOM
12347
CZ
ARG
B
238
70.579
6.802
−12.158
1.00
43.76
C


ATOM
12348
NH1
ARG
B
238
71.248
7.367
−13.166
1.00
42.21
N


ATOM
12351
NH2
ARG
B
238
70.835
7.198
−10.912
1.00
41.18
N


ATOM
12354
C
ARG
B
238
64.363
6.005
−13.678
1.00
33.04
C


ATOM
12355
O
ARG
B
238
63.812
4.963
−14.025
1.00
34.30
O


ATOM
12357
N
GLU
B
239
63.928
6.765
−12.678
1.00
33.01
N


ATOM
12358
CA
GLU
B
239
62.771
6.412
−11.861
1.00
33.59
C


ATOM
12360
CB
GLU
B
239
62.672
7.355
−10.657
1.00
35.06
C


ATOM
12363
CG
GLU
B
239
61.711
6.896
−9.561
1.00
42.92
C


ATOM
12366
CD
GLU
B
239
61.683
7.843
−8.362
1.00
53.62
C


ATOM
12367
OE1
GLU
B
239
62.683
8.567
−8.141
1.00
57.64
O


ATOM
12368
OE2
GLU
B
239
60.660
7.861
−7.637
1.00
57.88
O


ATOM
12369
C
GLU
B
239
61.486
6.464
−12.679
1.00
31.91
C


ATOM
12370
O
GLU
B
239
60.657
5.553
−12.614
1.00
32.53
O


ATOM
12372
N
THR
B
240
61.326
7.526
−13.459
1.00
29.79
N


ATOM
12373
CA
THR
B
240
60.177
7.652
−14.342
1.00
28.34
C


ATOM
12375
CB
THR
B
240
60.079
9.077
−14.916
1.00
27.73
C


ATOM
12377
OG1
THR
B
240
59.964
10.005
−13.833
1.00
27.37
O


ATOM
12379
CG2
THR
B
240
58.864
9.224
−15.820
1.00
25.05
C


ATOM
12383
C
THR
B
240
60.208
6.598
−15.463
1.00
28.19
C


ATOM
12384
O
THR
B
240
59.155
6.136
−15.905
1.00
28.32
O


ATOM
12386
N
SER
B
241
61.402
6.213
−15.911
1.00
28.18
N


ATOM
12387
CA
SER
B
241
61.535
5.111
−16.873
1.00
28.98
C


ATOM
12389
CB
SER
B
241
62.983
4.919
−17.322
1.00
29.08
C


ATOM
12392
OG
SER
B
241
63.289
5.768
−18.407
1.00
31.47
O


ATOM
12394
C
SER
B
241
61.005
3.797
−16.311
1.00
29.33
C


ATOM
12395
O
SER
B
241
60.295
3.078
−17.010
1.00
29.19
O


ATOM
12397
N
ARG
B
242
61.348
3.484
−15.061
1.00
30.11
N


ATOM
12398
CA
ARG
B
242
60.841
2.269
−14.409
1.00
31.50
C


ATOM
12400
CB
ARG
B
242
61.344
2.149
−12.966
1.00
32.60
C


ATOM
12403
CG
ARG
B
242
62.679
1.395
−12.830
1.00
39.21
C


ATOM
12406
CD
ARG
B
242
62.898
0.897
−11.394
1.00
46.39
C


ATOM
12409
NE
ARG
B
242
62.812
1.988
−10.415
1.00
52.27
N


ATOM
12411
CZ
ARG
B
242
63.847
2.686
−9.938
1.00
55.67
C


ATOM
12412
NH1
ARG
B
242
63.632
3.660
−9.055
1.00
55.49
N


ATOM
12415
NH2
ARG
B
242
65.095
2.424
−10.327
1.00
57.25
N


ATOM
12418
C
ARG
B
242
59.315
2.228
−14.433
1.00
30.76
C


ATOM
12419
O
ARG
B
242
58.713
1.273
−14.928
1.00
32.00
O


ATOM
12421
N
TRP
B
243
58.696
3.274
−13.904
1.00
29.80
N


ATOM
12422
CA
TRP
B
243
57.248
3.428
−13.980
1.00
28.69
C


ATOM
12424
CB
TRP
B
243
56.852
4.819
−13.486
1.00
28.10
C


ATOM
12427
CG
TRP
B
243
55.458
5.196
−13.808
1.00
24.46
C


ATOM
12428
CD1
TRP
B
243
54.346
4.874
−13.101
1.00
28.58
C


ATOM
12430
NE1
TRP
B
243
53.231
5.411
−13.702
1.00
29.43
N


ATOM
12432
CE2
TRP
B
243
53.619
6.092
−14.823
1.00
19.73
C


ATOM
12433
CD2
TRP
B
243
55.017
5.978
−14.921
1.00
19.48
C


ATOM
12434
CE3
TRP
B
243
55.668
6.587
−15.995
1.00
21.43
C


ATOM
12436
CZ3
TRP
B
243
54.913
7.286
−16.923
1.00
18.77
C


ATOM
12438
CH2
TRP
B
243
53.522
7.377
−16.799
1.00
22.46
C


ATOM
12440
CZ2
TRP
B
243
52.859
6.785
−15.755
1.00
21.65
C


ATOM
12442
C
TRP
B
243
56.734
3.213
−15.409
1.00
28.58
C


ATOM
12443
O
TRP
B
243
55.820
2.422
−15.632
1.00
29.05
O


ATOM
12445
N
TRP
B
244
57.338
3.917
−16.365
1.00
27.34
N


ATOM
12446
CA
TRP
B
244
56.883
3.900
−17.755
1.00
26.52
C


ATOM
12448
CB
TRP
B
244
57.659
4.940
−18.557
1.00
26.19
C


ATOM
12451
CG
TRP
B
244
57.218
5.115
−19.975
1.00
23.64
C


ATOM
12452
CD1
TRP
B
244
57.972
4.925
−21.083
1.00
24.42
C


ATOM
12454
NE1
TRP
B
244
57.238
5.200
−22.211
1.00
24.86
N


ATOM
12456
CE2
TRP
B
244
55.978
5.578
−21.840
1.00
22.00
C


ATOM
12457
CD2
TRP
B
244
55.925
5.532
−20.436
1.00
24.65
C


ATOM
12458
CE3
TRP
B
244
54.728
5.871
−19.795
1.00
24.31
C


ATOM
12460
CZ3
TRP
B
244
53.648
6.241
−20.565
1.00
21.89
C


ATOM
12462
CH2
TRP
B
244
53.733
6.273
−21.964
1.00
22.49
C


ATOM
12464
CZ2
TRP
B
244
54.889
5.950
−22.617
1.00
22.73
C


ATOM
12466
C
TRP
B
244
57.027
2.532
−18.415
1.00
26.35
C


ATOM
12467
O
TRP
B
244
56.131
2.086
−19.119
1.00
24.28
O


ATOM
12469
N
ARG
B
245
58.154
1.871
−18.187
1.00
28.23
N


ATOM
12470
CA
ARG
B
245
58.378
0.549
−18.771
1.00
30.73
C


ATOM
12472
CB
ARG
B
245
59.841
0.106
−18.608
1.00
31.78
C


ATOM
12475
CG
ARG
B
245
60.727
0.494
−19.799
1.00
37.60
C


ATOM
12478
CD
ARG
B
245
62.147
−0.077
−19.693
1.00
44.15
C


ATOM
12481
NE
ARG
B
245
63.034
0.795
−18.915
1.00
49.07
N


ATOM
12483
CZ
ARG
B
245
63.314
0.666
−17.613
1.00
53.61
C


ATOM
12484
NH1
ARG
B
245
64.140
1.534
−17.033
1.00
53.90
N


ATOM
12487
NH2
ARG
B
245
62.788
−0.314
−16.878
1.00
55.27
N


ATOM
12490
C
ARG
B
245
57.412
−0.504
−18.219
1.00
30.43
C


ATOM
12491
O
ARG
B
245
56.966
−1.371
−18.963
1.00
30.12
O


ATOM
12493
N
ARG
B
246
57.079
−0.413
−16.934
1.00
30.99
N


ATOM
12494
CA
ARG
B
246
56.122
−1.343
−16.312
1.00
31.60
C


ATOM
12496
CB
ARG
B
246
56.188
−1.288
−14.774
1.00
33.70
C


ATOM
12499
CG
ARG
B
246
57.010
−2.434
−14.159
1.00
42.26
C


ATOM
12502
CD
ARG
B
246
57.582
−2.098
−12.776
1.00
53.96
C


ATOM
12505
NE
ARG
B
246
56.564
−1.651
−11.817
1.00
63.57
N


ATOM
12507
CZ
ARG
B
246
56.753
−1.544
−10.498
1.00
69.41
C


ATOM
12508
NH1
ARG
B
246
57.921
−1.866
−9.941
1.00
70.68
N


ATOM
12511
NH2
ARG
B
246
55.757
−1.123
−9.722
1.00
71.34
N


ATOM
12514
C
ARG
B
246
54.698
−1.109
−16.791
1.00
28.27
C


ATOM
12515
O
ARG
B
246
53.949
−2.061
−16.987
1.00
28.49
O


ATOM
12517
N
VAL
B
247
54.325
0.148
−16.987
1.00
25.55
N


ATOM
12518
CA
VAL
B
247
53.032
0.460
−17.595
1.00
24.33
C


ATOM
12520
CB
VAL
B
247
52.780
1.978
−17.638
1.00
23.92
C


ATOM
12522
CG1
VAL
B
247
51.526
2.303
−18.429
1.00
22.50
C


ATOM
12526
CG2
VAL
B
247
52.675
2.526
−16.226
1.00
23.13
C


ATOM
12530
C
VAL
B
247
52.964
−0.153
−19.004
1.00
24.00
C


ATOM
12531
O
VAL
B
247
51.929
−0.693
−19.406
1.00
23.41
O


ATOM
12533
N
GLY
B
248
54.080
−0.071
−19.729
1.00
23.31
N


ATOM
12534
CA
GLY
B
248
54.277
−0.778
−20.994
1.00
23.13
C


ATOM
12537
C
GLY
B
248
53.256
−0.487
−22.074
1.00
23.62
C


ATOM
12538
O
GLY
B
248
52.871
−1.379
−22.826
1.00
23.94
O


ATOM
12540
N
LEU
B
249
52.840
0.767
−22.176
1.00
25.00
N


ATOM
12541
CA
LEU
B
249
51.691
1.123
−23.013
1.00
26.64
C


ATOM
12543
CB
LEU
B
249
51.045
2.413
−22.495
1.00
26.22
C


ATOM
12546
CG
LEU
B
249
49.518
2.511
−22.519
1.00
25.67
C


ATOM
12548
CD1
LEU
B
249
48.839
1.210
−22.074
1.00
22.73
C


ATOM
12552
CD2
LEU
B
249
49.081
3.691
−21.646
1.00
20.40
C


ATOM
12556
C
LEU
B
249
52.059
1.250
−24.495
1.00
28.64
C


ATOM
12557
O
LEU
B
249
51.299
0.815
−25.360
1.00
28.54
O


ATOM
12559
N
ALA
B
250
53.224
1.830
−24.785
1.00
30.89
N


ATOM
12560
CA
ALA
B
250
53.702
1.970
−26.170
1.00
31.80
C


ATOM
12562
CB
ALA
B
250
54.894
2.900
−26.222
1.00
32.53
C


ATOM
12566
C
ALA
B
250
54.071
0.625
−26.790
1.00
32.71
C


ATOM
12567
O
ALA
B
250
53.944
0.437
−28.007
1.00
34.22
O


ATOM
12569
N
THR
B
251
54.543
−0.295
−25.949
1.00
32.25
N


ATOM
12570
CA
THR
B
251
54.864
−1.651
−26.373
1.00
31.90
C


ATOM
12572
CB
THR
B
251
55.533
−2.449
−25.241
1.00
31.39
C


ATOM
12574
OG1
THR
B
251
56.767
−1.825
−24.883
1.00
32.89
O


ATOM
12576
CG2
THR
B
251
55.808
−3.873
−25.674
1.00
31.67
C


ATOM
12580
C
THR
B
251
53.616
−2.405
−26.784
1.00
32.40
C


ATOM
12581
O
THR
B
251
53.611
−3.087
−27.811
1.00
34.18
O


ATOM
12583
N
LYS
B
252
52.562
−2.281
−25.981
1.00
32.28
N


ATOM
12584
CA
LYS
B
252
51.356
−3.090
−26.164
1.00
32.62
C


ATOM
12586
CB
LYS
B
252
50.634
−3.289
−24.827
1.00
32.87
C


ATOM
12589
CG
LYS
B
252
51.444
−4.094
−23.797
1.00
35.03
C


ATOM
12592
CD
LYS
B
252
51.356
−5.599
−24.043
1.00
37.32
C


ATOM
12595
CE
LYS
B
252
52.373
−6.367
−23.209
1.00
39.70
C


ATOM
12598
NZ
LYS
B
252
52.202
−7.840
−23.336
1.00
40.83
N


ATOM
12602
C
LYS
B
252
50.409
−2.494
−27.197
1.00
32.21
C


ATOM
12603
O
LYS
B
252
49.682
−3.225
−27.859
1.00
33.55
O


ATOM
12605
N
LEU
B
253
50.420
−1.172
−27.330
1.00
31.92
N


ATOM
12606
CA
LEU
B
253
49.608
−0.484
−28.327
1.00
32.16
C


ATOM
12608
CB
LEU
B
253
48.999
0.785
−27.730
1.00
32.43
C


ATOM
12611
CG
LEU
B
253
48.096
0.552
−26.509
1.00
32.13
C


ATOM
12613
CD1
LEU
B
253
47.870
1.842
−25.735
1.00
29.32
C


ATOM
12617
CD2
LEU
B
253
46.773
−0.064
−26.920
1.00
31.21
C


ATOM
12621
C
LEU
B
253
50.462
−0.155
−29.553
1.00
32.55
C


ATOM
12622
O
LEU
B
253
51.409
0.639
−29.475
1.00
32.24
O


ATOM
12624
N
HIS
B
254
50.123
−0.783
−30.678
1.00
32.82
N


ATOM
12625
CA
HIS
B
254
50.958
−0.743
−31.882
1.00
34.02
C


ATOM
12627
CB
HIS
B
254
50.508
−1.819
−32.885
1.00
34.99
C


ATOM
12630
CG
HIS
B
254
49.203
−1.517
−33.562
1.00
40.81
C


ATOM
12631
ND1
HIS
B
254
48.003
−1.473
−32.884
1.00
46.40
N


ATOM
12633
CE1
HIS
B
254
47.030
−1.188
−33.733
1.00
47.23
C


ATOM
12635
NE2
HIS
B
254
47.554
−1.046
−34.937
1.00
45.87
N


ATOM
12637
CD2
HIS
B
254
48.911
−1.249
−34.858
1.00
45.87
C


ATOM
12639
C
HIS
B
254
50.988
0.638
−32.546
1.00
33.59
C


ATOM
12640
O
HIS
B
254
51.977
1.004
−33.175
1.00
33.49
O


ATOM
12642
N
PHE
B
255
49.906
1.397
−32.399
1.00
33.23
N


ATOM
12643
CA
PHE
B
255
49.822
2.750
−32.954
1.00
32.76
C


ATOM
12645
CB
PHE
B
255
48.352
3.162
−33.115
1.00
32.23
C


ATOM
12648
CG
PHE
B
255
47.639
3.375
−31.806
1.00
34.07
C


ATOM
12649
CD1
PHE
B
255
47.714
4.598
−31.149
1.00
35.73
C


ATOM
12651
CE1
PHE
B
255
47.078
4.793
−29.932
1.00
36.53
C


ATOM
12653
CZ
PHE
B
255
46.358
3.758
−29.356
1.00
35.90
C


ATOM
12655
CE2
PHE
B
255
46.281
2.531
−30.000
1.00
34.97
C


ATOM
12657
CD2
PHE
B
255
46.919
2.346
−31.216
1.00
35.32
C


ATOM
12659
C
PHE
B
255
50.569
3.795
−32.100
1.00
32.70
C


ATOM
12660
O
PHE
B
255
50.889
4.879
−32.587
1.00
33.05
O


ATOM
12662
N
ALA
B
256
50.846
3.466
−30.837
1.00
32.71
N


ATOM
12663
CA
ALA
B
256
51.324
4.451
−29.855
1.00
32.49
C


ATOM
12665
CB
ALA
B
256
51.100
3.931
−28.435
1.00
31.46
C


ATOM
12669
C
ALA
B
256
52.791
4.857
−30.031
1.00
32.88
C


ATOM
12670
O
ALA
B
256
53.655
4.023
−30.300
1.00
31.57
O


ATOM
12672
N
ARG
B
257
53.047
6.155
−29.863
1.00
34.38
N


ATOM
12673
CA
ARG
B
257
54.405
6.705
−29.842
1.00
34.26
C


ATOM
12675
CB
ARG
B
257
54.406
8.155
−30.345
1.00
34.10
C


ATOM
12678
CG
ARG
B
257
53.944
8.342
−31.792
1.00
36.63
C


ATOM
12681
CD
ARG
B
257
53.800
9.830
−32.132
1.00
38.52
C


ATOM
12684
NE
ARG
B
257
52.619
10.425
−31.501
1.00
41.28
N


ATOM
12686
CZ
ARG
B
257
52.429
11.732
−31.304
1.00
46.94
C


ATOM
12687
NH1
ARG
B
257
53.344
12.624
−31.678
1.00
49.83
N


ATOM
12690
NH2
ARG
B
257
51.311
12.154
−30.714
1.00
47.41
N


ATOM
12693
C
ARG
B
257
54.952
6.678
−28.416
1.00
33.80
C


ATOM
12694
O
ARG
B
257
54.274
7.127
−27.484
1.00
33.65
O


ATOM
12696
N
ASP
B
258
56.166
6.147
−28.253
1.00
33.38
N


ATOM
12697
CA
ASP
B
258
56.916
6.253
−26.992
1.00
32.84
C


ATOM
12699
CB
ASP
B
258
57.869
5.060
−26.821
1.00
32.27
C


ATOM
12702
CG
ASP
B
258
58.615
5.079
−25.484
1.00
33.79
C


ATOM
12703
OD1
ASP
B
258
58.403
6.016
−24.682
1.00
27.94
O


ATOM
12704
OD2
ASP
B
258
59.416
4.151
−25.230
1.00
34.26
O


ATOM
12705
C
ASP
B
258
57.701
7.580
−26.970
1.00
32.65
C


ATOM
12706
O
ASP
B
258
58.719
7.723
−27.655
1.00
32.02
O


ATOM
12708
N
ARG
B
259
57.226
8.532
−26.169
1.00
32.74
N


ATOM
12709
CA
ARG
B
259
57.801
9.880
−26.112
1.00
33.33
C


ATOM
12711
CB
ARG
B
259
56.780
10.881
−26.663
1.00
34.08
C


ATOM
12714
CG
ARG
B
259
56.680
10.902
−28.179
1.00
39.83
C


ATOM
12717
CD
ARG
B
259
57.587
11.969
−28.800
1.00
47.10
C


ATOM
12720
NE
ARG
B
259
57.378
12.074
−30.243
1.00
52.94
N


ATOM
12722
CZ
ARG
B
259
57.833
11.203
−31.149
1.00
57.88
C


ATOM
12723
NH1
ARG
B
259
58.545
10.137
−30.782
1.00
58.91
N


ATOM
12726
NH2
ARG
B
259
57.573
11.396
−32.439
1.00
58.39
N


ATOM
12729
C
ARG
B
259
58.219
10.286
−24.688
1.00
31.97
C


ATOM
12730
O
ARG
B
259
58.035
11.444
−24.289
1.00
31.74
O


ATOM
12732
N
LEU
B
260
58.787
9.345
−23.930
1.00
29.99
N


ATOM
12733
CA
LEU
B
260
59.111
9.599
−22.523
1.00
28.89
C


ATOM
12735
CB
LEU
B
260
59.407
8.294
−21.760
1.00
28.53
C


ATOM
12738
CG
LEU
B
260
59.795
8.420
−20.273
1.00
27.86
C


ATOM
12740
CD1
LEU
B
260
58.752
9.159
−19.462
1.00
23.05
C


ATOM
12744
CD2
LEU
B
260
60.025
7.057
−19.673
1.00
31.60
C


ATOM
12748
C
LEU
B
260
60.292
10.552
−22.408
1.00
27.72
C


ATOM
12749
O
LEU
B
260
60.210
11.582
−21.729
1.00
28.38
O


ATOM
12751
N
ILE
B
261
61.382
10.216
−23.085
1.00
25.46
N


ATOM
12752
CA
ILE
B
261
62.592
11.029
−23.012
1.00
24.56
C


ATOM
12754
CB
ILE
B
261
63.741
10.422
−23.854
1.00
24.32
C


ATOM
12756
CG1
ILE
B
261
64.113
9.023
−23.328
1.00
26.66
C


ATOM
12759
CD1
ILE
B
261
64.701
8.083
−24.377
1.00
24.42
C


ATOM
12763
CG2
ILE
B
261
64.954
11.334
−23.817
1.00
20.66
C


ATOM
12767
C
ILE
B
261
62.277
12.467
−23.460
1.00
23.83
C


ATOM
12768
O
ILE
B
261
62.652
13.432
−22.788
1.00
21.34
O


ATOM
12770
N
GLU
B
262
61.562
12.595
−24.578
1.00
23.36
N


ATOM
12771
CA
GLU
B
262
61.151
13.902
−25.087
1.00
23.51
C


ATOM
12773
CB
GLU
B
262
60.390
13.764
−26.413
1.00
24.09
C


ATOM
12776
CG
GLU
B
262
61.253
13.340
−27.603
1.00
28.89
C


ATOM
12779
CD
GLU
B
262
61.363
11.821
−27.788
1.00
36.03
C


ATOM
12780
OE1
GLU
B
262
61.521
11.079
−26.784
1.00
35.08
O


ATOM
12781
OE2
GLU
B
262
61.309
11.372
−28.958
1.00
41.14
O


ATOM
12782
C
GLU
B
262
60.283
14.628
−24.053
1.00
22.99
C


ATOM
12783
O
GLU
B
262
60.440
15.832
−23.827
1.00
21.89
O


ATOM
12785
N
SER
B
263
59.374
13.888
−23.421
1.00
22.63
N


ATOM
12786
CA
SER
B
263
58.494
14.468
−22.409
1.00
22.10
C


ATOM
12788
CB
SER
B
263
57.418
13.472
−21.970
1.00
22.50
C


ATOM
12791
OG
SER
B
263
56.408
13.370
−22.961
1.00
24.94
O


ATOM
12793
C
SER
B
263
59.286
14.958
−21.209
1.00
20.61
C


ATOM
12794
O
SER
B
263
58.994
16.009
−20.658
1.00
18.48
O


ATOM
12796
N
PHE
B
264
60.302
14.205
−20.810
1.00
21.00
N


ATOM
12797
CA
PHE
B
264
61.139
14.645
−19.703
1.00
20.52
C


ATOM
12799
CB
PHE
B
264
62.095
13.548
−19.246
1.00
19.25
C


ATOM
12802
CG
PHE
B
264
62.627
13.797
−17.888
1.00
18.67
C


ATOM
12803
CD1
PHE
B
264
61.911
13.396
−16.771
1.00
17.30
C


ATOM
12805
CE1
PHE
B
264
62.373
13.658
−15.513
1.00
15.98
C


ATOM
12807
CZ
PHE
B
264
63.557
14.354
−15.356
1.00
20.53
C


ATOM
12809
CE2
PHE
B
264
64.266
14.781
−16.461
1.00
18.60
C


ATOM
12811
CD2
PHE
B
264
63.793
14.513
−17.717
1.00
19.97
C


ATOM
12813
C
PHE
B
264
61.934
15.906
−20.055
1.00
21.18
C


ATOM
12814
O
PHE
B
264
62.070
16.811
−19.235
1.00
21.13
O


ATOM
12816
N
TYR
B
265
62.464
15.946
−21.273
1.00
22.16
N


ATOM
12817
CA
TYR
B
265
63.206
17.098
−21.773
1.00
23.19
C


ATOM
12819
CB
TYR
B
265
63.668
16.832
−23.210
1.00
23.48
C


ATOM
12822
CG
TYR
B
265
64.017
18.064
−24.015
1.00
29.92
C


ATOM
12823
CD1
TYR
B
265
65.240
18.714
−23.835
1.00
35.18
C


ATOM
12825
CE1
TYR
B
265
65.574
19.839
−24.583
1.00
37.23
C


ATOM
12827
CZ
TYR
B
265
64.684
20.320
−25.530
1.00
38.30
C


ATOM
12828
OH
TYR
B
265
65.016
21.436
−26.269
1.00
42.78
O


ATOM
12830
CE2
TYR
B
265
63.462
19.687
−25.729
1.00
35.17
C


ATOM
12832
CD2
TYR
B
265
63.139
18.565
−24.977
1.00
32.59
C


ATOM
12834
C
TYR
B
265
62.326
18.337
−21.701
1.00
22.75
C


ATOM
12835
O
TYR
B
265
62.764
19.406
−21.267
1.00
23.58
O


ATOM
12837
N
TRP
B
266
61.078
18.166
−22.119
1.00
21.59
N


ATOM
12838
CA
TRP
B
266
60.050
19.190
−21.990
1.00
20.37
C


ATOM
12840
CB
TRP
B
266
58.734
18.643
−22.526
1.00
20.30
C


ATOM
12843
CG
TRP
B
266
57.601
19.570
−22.424
1.00
20.24
C


ATOM
12844
CD1
TRP
B
266
56.763
19.729
−21.363
1.00
20.28
C


ATOM
12846
NE1
TRP
B
266
55.814
20.679
−21.651
1.00
20.11
N


ATOM
12848
CE2
TRP
B
266
56.028
21.145
−22.922
1.00
22.59
C


ATOM
12849
CD2
TRP
B
266
57.145
20.464
−23.438
1.00
21.18
C


ATOM
12850
CE3
TRP
B
266
57.573
20.754
−24.736
1.00
22.79
C


ATOM
12852
CZ3
TRP
B
266
56.885
21.706
−25.464
1.00
25.95
C


ATOM
12854
CH2
TRP
B
266
55.776
22.368
−24.925
1.00
24.56
C


ATOM
12856
CZ2
TRP
B
266
55.332
22.104
−23.659
1.00
25.55
C


ATOM
12858
C
TRP
B
266
59.854
19.605
−20.547
1.00
19.47
C


ATOM
12859
O
TRP
B
266
59.797
20.787
−20.244
1.00
20.67
O


ATOM
12861
N
ALA
B
267
59.736
18.623
−19.660
1.00
18.53
N


ATOM
12862
CA
ALA
B
267
59.499
18.882
−18.240
1.00
17.60
C


ATOM
12864
CB
ALA
B
267
59.270
17.585
−17.505
1.00
17.46
C


ATOM
12868
C
ALA
B
267
60.631
19.664
−17.585
1.00
17.74
C


ATOM
12869
O
ALA
B
267
60.398
20.395
−16.633
1.00
18.27
O


ATOM
12871
N
VAL
B
268
61.850
19.505
−18.096
1.00
18.33
N


ATOM
12872
CA
VAL
B
268
63.018
20.236
−17.592
1.00
17.43
C


ATOM
12874
CB
VAL
B
268
64.332
19.613
−18.123
1.00
17.15
C


ATOM
12876
CG1
VAL
B
268
65.522
20.542
−17.903
1.00
13.64
C


ATOM
12880
CG2
VAL
B
268
64.566
18.259
−17.464
1.00
15.60
C


ATOM
12884
C
VAL
B
268
62.943
21.717
−17.968
1.00
17.91
C


ATOM
12885
O
VAL
B
268
63.488
22.575
−17.265
1.00
18.01
O


ATOM
12887
N
GLY
B
269
62.282
22.006
−19.087
1.00
18.16
N


ATOM
12888
CA
GLY
B
269
62.007
23.381
−19.496
1.00
17.58
C


ATOM
12891
C
GLY
B
269
61.069
24.062
−18.523
1.00
17.55
C


ATOM
12892
O
GLY
B
269
61.250
25.239
−18.206
1.00
19.16
O


ATOM
12894
N
VAL
B
270
60.090
23.310
−18.022
1.00
16.05
N


ATOM
12895
CA
VAL
B
270
59.058
23.870
−17.169
1.00
15.65
C


ATOM
12897
CB
VAL
B
270
57.799
22.998
−17.154
1.00
15.67
C


ATOM
12899
CG1
VAL
B
270
56.784
23.542
−16.158
1.00
12.64
C


ATOM
12903
CG2
VAL
B
270
57.194
22.939
−18.546
1.00
14.23
C


ATOM
12907
C
VAL
B
270
59.532
24.105
−15.741
1.00
16.19
C


ATOM
12908
O
VAL
B
270
59.205
25.133
−15.142
1.00
17.24
O


ATOM
12910
N
ALA
B
271
60.282
23.156
−15.192
1.00
16.15
N


ATOM
12911
CA
ALA
B
271
60.764
23.247
−13.806
1.00
16.22
C


ATOM
12913
CB
ALA
B
271
59.785
22.566
−12.858
1.00
14.70
C


ATOM
12917
C
ALA
B
271
62.161
22.621
−13.702
1.00
16.92
C


ATOM
12918
O
ALA
B
271
62.299
21.406
−13.577
1.00
16.47
O


ATOM
12920
N
PHE
B
272
63.188
23.464
−13.756
1.00
17.73
N


ATOM
12921
CA
PHE
B
272
64.564
22.998
−13.927
1.00
19.08
C


ATOM
12923
CB
PHE
B
272
65.321
23.942
−14.861
1.00
19.69
C


ATOM
12926
CG
PHE
B
272
65.744
25.224
−14.213
1.00
20.99
C


ATOM
12927
CD1
PHE
B
272
66.957
25.309
−13.544
1.00
24.32
C


ATOM
12929
CE1
PHE
B
272
67.353
26.488
−12.947
1.00
24.57
C


ATOM
12931
CZ
PHE
B
272
66.535
27.599
−13.014
1.00
25.03
C


ATOM
12933
CE2
PHE
B
272
65.324
27.530
−13.679
1.00
22.70
C


ATOM
12935
CD2
PHE
B
272
64.934
26.343
−14.272
1.00
22.88
C


ATOM
12937
C
PHE
B
272
65.344
22.850
−12.625
1.00
19.52
C


ATOM
12938
O
PHE
B
272
66.277
22.051
−12.553
1.00
19.57
O


ATOM
12940
N
GLU
B
273
64.971
23.624
−11.608
1.00
20.16
N


ATOM
12941
CA
GLU
B
273
65.741
23.675
−10.371
1.00
20.65
C


ATOM
12943
CB
GLU
B
273
65.181
24.727
−9.405
1.00
20.99
C


ATOM
12946
CG
GLU
B
273
65.316
26.176
−9.883
1.00
22.85
C


ATOM
12949
CD
GLU
B
273
64.088
26.682
−10.628
1.00
27.28
C


ATOM
12950
OE1
GLU
B
273
63.673
27.837
−10.391
1.00
31.35
O


ATOM
12951
OE2
GLU
B
273
63.530
25.929
−11.450
1.00
31.83
O


ATOM
12952
C
GLU
B
273
65.750
22.299
−9.711
1.00
20.45
C


ATOM
12953
O
GLU
B
273
64.729
21.625
−9.670
1.00
21.36
O


ATOM
12955
N
PRO
B
274
66.904
21.873
−9.188
1.00
20.01
N


ATOM
12956
CA
PRO
B
274
67.015
20.492
−8.703
1.00
19.83
C


ATOM
12958
CB
PRO
B
274
68.357
20.478
−7.951
1.00
19.83
C


ATOM
12961
CG
PRO
B
274
68.845
21.897
−7.936
1.00
19.28
C


ATOM
12964
CD
PRO
B
274
68.165
22.615
−9.037
1.00
19.35
C


ATOM
12967
C
PRO
B
274
65.872
20.024
−7.799
1.00
19.29
C


ATOM
12968
O
PRO
B
274
65.471
18.867
−7.878
1.00
21.29
O


ATOM
12969
N
GLN
B
275
65.340
20.908
−6.967
1.00
19.08
N


ATOM
12970
CA
GLN
B
275
64.287
20.532
−6.007
1.00
19.66
C


ATOM
12972
CB
GLN
B
275
64.018
21.677
−5.021
1.00
19.32
C


ATOM
12975
CG
GLN
B
275
63.679
23.030
−5.650
1.00
21.51
C


ATOM
12978
CD
GLN
B
275
64.905
23.899
−5.919
1.00
26.18
C


ATOM
12979
OE1
GLN
B
275
65.947
23.405
−6.351
1.00
25.97
O


ATOM
12980
NE2
GLN
B
275
64.777
25.204
−5.677
1.00
27.95
N


ATOM
12983
C
GLN
B
275
62.959
20.077
−6.641
1.00
20.56
C


ATOM
12984
O
GLN
B
275
62.171
19.376
−5.992
1.00
20.08
O


ATOM
12986
N
TYR
B
276
62.723
20.444
−7.903
1.00
20.07
N


ATOM
12987
CA
TYR
B
276
61.442
20.181
−8.554
1.00
20.27
C


ATOM
12989
CB
TYR
B
276
61.105
21.332
−9.508
1.00
20.86
C


ATOM
12992
CG
TYR
B
276
60.869
22.660
−8.810
1.00
22.46
C


ATOM
12993
CD1
TYR
B
276
59.986
22.761
−7.738
1.00
21.03
C


ATOM
12995
CE1
TYR
B
276
59.762
23.969
−7.108
1.00
21.89
C


ATOM
12997
CZ
TYR
B
276
60.410
25.098
−7.550
1.00
25.18
C


ATOM
12998
OH
TYR
B
276
60.176
26.299
−6.921
1.00
31.83
O


ATOM
13000
CE2
TYR
B
276
61.284
25.032
−8.611
1.00
23.66
C


ATOM
13002
CD2
TYR
B
276
61.507
23.819
−9.238
1.00
24.15
C


ATOM
13004
C
TYR
B
276
61.360
18.821
−9.278
1.00
20.12
C


ATOM
13005
O
TYR
B
276
60.750
18.697
−10.344
1.00
19.49
O


ATOM
13007
N
SER
B
277
61.948
17.791
−8.682
1.00
19.98
N


ATOM
13008
CA
SER
B
277
61.841
16.447
−9.234
1.00
19.57
C


ATOM
13010
CB
SER
B
277
62.556
15.437
−8.338
1.00
18.90
C


ATOM
13013
OG
SER
B
277
63.950
15.509
−8.547
1.00
19.52
O


ATOM
13015
C
SER
B
277
60.382
16.041
−9.424
1.00
20.31
C


ATOM
13016
O
SER
B
277
60.011
15.551
−10.495
1.00
21.30
O


ATOM
13018
N
ASP
B
278
59.559
16.251
−8.394
1.00
20.02
N


ATOM
13019
CA
ASP
B
278
58.149
15.879
−8.460
1.00
20.05
C


ATOM
13021
CB
ASP
B
278
57.404
16.258
−7.178
1.00
21.33
C


ATOM
13024
CG
ASP
B
278
57.767
15.380
−6.002
1.00
24.28
C


ATOM
13025
OD1
ASP
B
278
58.485
14.374
−6.188
1.00
32.22
O


ATOM
13026
OD2
ASP
B
278
57.330
15.707
−4.876
1.00
30.56
O


ATOM
13027
C
ASP
B
278
57.479
16.551
−9.642
1.00
19.68
C


ATOM
13028
O
ASP
B
278
56.819
15.893
−10.447
1.00
19.85
O


ATOM
13030
N
CYS
B
279
57.652
17.863
−9.754
1.00
19.27
N


ATOM
13031
CA
CYS
B
279
57.076
18.586
−10.871
1.00
19.01
C


ATOM
13033
CB
CYS
B
279
57.382
20.076
−10.783
1.00
19.07
C


ATOM
13036
SG
CYS
B
279
56.476
21.038
−11.994
1.00
21.99
S


ATOM
13038
C
CYS
B
279
57.562
18.010
−12.203
1.00
18.56
C


ATOM
13039
O
CYS
B
279
56.762
17.799
−13.108
1.00
18.25
O


ATOM
13041
N
ARG
B
280
58.859
17.730
−12.310
1.00
18.41
N


ATOM
13042
CA
ARG
B
280
59.416
17.197
−13.561
1.00
18.94
C


ATOM
13044
CB
ARG
B
280
60.945
17.080
−13.505
1.00
18.75
C


ATOM
13047
CG
ARG
B
280
61.656
18.382
−13.810
1.00
18.50
C


ATOM
13050
CD
ARG
B
280
63.150
18.188
−13.957
1.00
17.74
C


ATOM
13053
NE
ARG
B
280
63.753
17.708
−12.721
1.00
13.97
N


ATOM
13055
CZ
ARG
B
280
64.014
18.462
−11.661
1.00
15.77
C


ATOM
13056
NH1
ARG
B
280
63.726
19.756
−11.660
1.00
18.71
N


ATOM
13059
NH2
ARG
B
280
64.562
17.909
−10.582
1.00
20.38
N


ATOM
13062
C
ARG
B
280
58.821
15.851
−13.902
1.00
19.15
C


ATOM
13063
O
ARG
B
280
58.471
15.601
−15.052
1.00
18.90
O


ATOM
13065
N
ASN
B
281
58.708
14.991
−12.895
1.00
20.06
N


ATOM
13066
CA
ASN
B
281
58.178
13.648
−13.090
1.00
20.75
C


ATOM
13068
CB
ASN
B
281
58.464
12.784
−11.860
1.00
20.99
C


ATOM
13071
CG
ASN
B
281
59.972
12.554
−11.641
1.00
25.66
C


ATOM
13072
OD1
ASN
B
281
60.746
12.419
−12.596
1.00
28.16
O


ATOM
13073
ND2
ASN
B
281
60.385
12.520
−10.381
1.00
25.36
N


ATOM
13076
C
ASN
B
281
56.689
13.693
−13.408
1.00
20.49
C


ATOM
13077
O
ASN
B
281
56.209
12.991
−14.296
1.00
19.74
O


ATOM
13079
N
SER
B
282
55.970
14.555
−12.702
1.00
20.45
N


ATOM
13080
CA
SER
B
282
54.537
14.701
−12.901
1.00
20.12
C


ATOM
13082
CB
SER
B
282
53.956
15.651
−11.854
1.00
20.65
C


ATOM
13085
OG
SER
B
282
52.595
15.359
−11.618
1.00
22.82
O


ATOM
13087
C
SER
B
282
54.215
15.190
−14.312
1.00
19.43
C


ATOM
13088
O
SER
B
282
53.323
14.652
−14.974
1.00
19.76
O


ATOM
13090
N
VAL
B
283
54.953
16.195
−14.775
1.00
18.29
N


ATOM
13091
CA
VAL
B
283
54.768
16.726
−16.127
1.00
16.97
C


ATOM
13093
CB
VAL
B
283
55.511
18.073
−16.319
1.00
16.72
C


ATOM
13095
CG1
VAL
B
283
55.569
18.478
−17.788
1.00
16.10
C


ATOM
13099
CG2
VAL
B
283
54.837
19.148
−15.509
1.00
11.99
C


ATOM
13103
C
VAL
B
283
55.190
15.716
−17.196
1.00
17.36
C


ATOM
13104
O
VAL
B
283
54.528
15.593
−18.229
1.00
17.90
O


ATOM
13106
N
ALA
B
284
56.275
14.988
−16.947
1.00
17.45
N


ATOM
13107
CA
ALA
B
284
56.758
13.992
−17.906
1.00
17.41
C


ATOM
13109
CB
ALA
B
284
58.094
13.417
−17.466
1.00
15.89
C


ATOM
13113
C
ALA
B
284
55.732
12.878
−18.107
1.00
18.47
C


ATOM
13114
O
ALA
B
284
55.487
12.456
−19.237
1.00
19.32
O


ATOM
13116
N
LYS
B
285
55.128
12.422
−17.009
1.00
19.17
N


ATOM
13117
CA
LYS
B
285
54.095
11.380
−17.050
1.00
19.22
C


ATOM
13119
CB
LYS
B
285
53.741
10.919
−15.630
1.00
19.40
C


ATOM
13122
CG
LYS
B
285
54.872
10.185
−14.896
1.00
21.47
C


ATOM
13125
CD
LYS
B
285
54.543
10.003
−13.422
1.00
26.37
C


ATOM
13128
CE
LYS
B
285
55.594
9.183
−12.694
1.00
30.54
C


ATOM
13131
NZ
LYS
B
285
55.085
8.669
−11.388
1.00
33.42
N


ATOM
13135
C
LYS
B
285
52.829
11.855
−17.767
1.00
18.79
C


ATOM
13136
O
LYS
B
285
52.285
11.159
−18.627
1.00
17.49
O


ATOM
13138
N
MET
B
286
52.364
13.046
−17.416
1.00
18.67
N


ATOM
13139
CA
MET
B
286
51.183
13.603
−18.066
1.00
17.87
C


ATOM
13141
CB
MET
B
286
50.803
14.943
−17.441
1.00
17.79
C


ATOM
13144
CG
MET
B
286
50.283
14.840
−16.008
1.00
15.56
C


ATOM
13147
SD
MET
B
286
49.110
13.495
−15.755
1.00
17.16
S


ATOM
13148
CE
MET
B
286
50.186
12.126
−15.346
1.00
13.71
C


ATOM
13152
C
MET
B
286
51.409
13.752
−19.558
1.00
18.49
C


ATOM
13153
O
MET
B
286
50.586
13.306
−20.354
1.00
20.49
O


ATOM
13155
N
PHE
B
287
52.537
14.336
−19.946
1.00
18.40
N


ATOM
13156
CA
PHE
B
287
52.808
14.567
−21.362
1.00
18.66
C


ATOM
13158
CB
PHE
B
287
54.040
15.469
−21.547
1.00
20.00
C


ATOM
13161
CG
PHE
B
287
54.026
16.310
−22.817
1.00
24.97
C


ATOM
13162
CD1
PHE
B
287
52.947
16.300
−23.705
1.00
30.68
C


ATOM
13164
CE1
PHE
B
287
52.954
17.078
−24.852
1.00
30.97
C


ATOM
13166
CZ
PHE
B
287
54.019
17.892
−25.119
1.00
32.06
C


ATOM
13168
CE2
PHE
B
287
55.089
17.933
−24.243
1.00
31.07
C


ATOM
13170
CD2
PHE
B
287
55.087
17.151
−23.097
1.00
28.48
C


ATOM
13172
C
PHE
B
287
52.978
13.252
−22.116
1.00
17.87
C


ATOM
13173
O
PHE
B
287
52.718
13.175
−23.315
1.00
16.55
O


ATOM
13175
N
SER
B
288
53.403
12.206
−21.413
1.00
18.39
N


ATOM
13176
CA
SER
B
288
53.520
10.894
−22.037
1.00
18.35
C


ATOM
13178
CB
SER
B
288
54.306
9.946
−21.154
1.00
17.99
C


ATOM
13181
OG
SER
B
288
55.617
10.437
−20.969
1.00
22.94
O


ATOM
13183
C
SER
B
288
52.136
10.333
−22.343
1.00
18.48
C


ATOM
13184
O
SER
B
288
51.889
9.873
−23.451
1.00
19.11
O


ATOM
13186
N
PHE
B
289
51.232
10.396
−21.372
1.00
18.18
N


ATOM
13187
CA
PHE
B
289
49.855
9.965
−21.594
1.00
18.11
C


ATOM
13189
CB
PHE
B
289
49.042
9.977
−20.295
1.00
18.57
C


ATOM
13192
CG
PHE
B
289
49.139
8.711
−19.504
1.00
18.20
C


ATOM
13193
CD1
PHE
B
289
48.556
7.545
−19.968
1.00
18.92
C


ATOM
13195
CE1
PHE
B
289
48.646
6.365
−19.241
1.00
21.23
C


ATOM
13197
CZ
PHE
B
289
49.309
6.347
−18.025
1.00
22.20
C


ATOM
13199
CE2
PHE
B
289
49.890
7.506
−17.546
1.00
23.50
C


ATOM
13201
CD2
PHE
B
289
49.800
8.684
−18.286
1.00
24.62
C


ATOM
13203
C
PHE
B
289
49.168
10.844
−22.630
1.00
18.51
C


ATOM
13204
O
PHE
B
289
48.442
10.339
−23.484
1.00
19.51
O


ATOM
13206
N
VAL
B
290
49.387
12.153
−22.559
1.00
18.16
N


ATOM
13207
CA
VAL
B
290
48.790
13.074
−23.534
1.00
17.57
C


ATOM
13209
CB
VAL
B
290
49.261
14.532
−23.312
1.00
17.57
C


ATOM
13211
CG1
VAL
B
290
48.855
15.404
−24.498
1.00
17.88
C


ATOM
13215
CG2
VAL
B
290
48.700
15.097
−22.002
1.00
13.02
C


ATOM
13219
C
VAL
B
290
49.134
12.653
−24.970
1.00
17.90
C


ATOM
13220
O
VAL
B
290
48.286
12.683
−25.866
1.00
18.78
O


ATOM
13222
N
THR
B
291
50.384
12.259
−25.176
1.00
17.74
N


ATOM
13223
CA
THR
B
291
50.855
11.837
−26.483
1.00
17.95
C


ATOM
13225
CB
THR
B
291
52.333
11.447
−26.420
1.00
18.41
C


ATOM
13227
OG1
THR
B
291
53.124
12.629
−26.260
1.00
21.17
O


ATOM
13229
CG2
THR
B
291
52.762
10.727
−27.680
1.00
17.99
C


ATOM
13233
C
THR
B
291
50.055
10.651
−26.998
1.00
18.29
C


ATOM
13234
O
THR
B
291
49.633
10.632
−28.159
1.00
19.30
O


ATOM
13236
N
ILE
B
292
49.840
9.661
−26.138
1.00
17.08
N


ATOM
13237
CA
ILE
B
292
49.097
8.480
−26.549
1.00
16.79
C


ATOM
13239
CB
ILE
B
292
49.217
7.343
−25.528
1.00
17.07
C


ATOM
13241
CG1
ILE
B
292
50.680
6.927
−25.377
1.00
15.64
C


ATOM
13244
CD1
ILE
B
292
50.876
5.738
−24.488
1.00
16.03
C


ATOM
13248
CG2
ILE
B
292
48.374
6.138
−25.976
1.00
15.60
C


ATOM
13252
C
ILE
B
292
47.619
8.794
−26.788
1.00
16.39
C


ATOM
13253
O
ILE
B
292
47.049
8.387
−27.801
1.00
15.98
O


ATOM
13255
N
ILE
B
293
47.007
9.516
−25.856
1.00
16.87
N


ATOM
13256
CA
ILE
B
293
45.576
9.813
−25.931
1.00
16.97
C


ATOM
13258
CB
ILE
B
293
45.038
10.467
−24.635
1.00
17.10
C


ATOM
13260
CG1
ILE
B
293
45.298
9.575
−23.415
1.00
15.92
C


ATOM
13263
CD1
ILE
B
293
44.447
8.382
−23.354
1.00
19.33
C


ATOM
13267
CG2
ILE
B
293
43.545
10.770
−24.769
1.00
12.01
C


ATOM
13271
C
ILE
B
293
45.272
10.737
−27.109
1.00
18.45
C


ATOM
13272
O
ILE
B
293
44.232
10.608
−27.746
1.00
19.12
O


ATOM
13274
N
ASP
B
294
46.176
11.666
−27.402
1.00
20.04
N


ATOM
13275
CA
ASP
B
294
46.022
12.503
−28.589
1.00
20.87
C


ATOM
13277
CB
ASP
B
294
47.143
13.550
−28.676
1.00
21.91
C


ATOM
13280
CG
ASP
B
294
46.999
14.474
−29.880
1.00
25.51
C


ATOM
13281
OD1
ASP
B
294
45.859
14.741
−30.330
1.00
37.55
O


ATOM
13282
OD2
ASP
B
294
48.037
14.933
−30.384
1.00
32.86
O


ATOM
13283
C
ASP
B
294
45.990
11.621
−29.842
1.00
20.09
C


ATOM
13284
O
ASP
B
294
45.084
11.748
−30.655
1.00
20.67
O


ATOM
13286
N
ASP
B
295
46.967
10.725
−29.985
1.00
18.78
N


ATOM
13287
CA
ASP
B
295
46.981
9.780
−31.096
1.00
18.93
C


ATOM
13289
CB
ASP
B
295
48.031
8.683
−30.888
1.00
20.45
C


ATOM
13292
CG
ASP
B
295
49.444
9.154
−31.119
1.00
24.00
C


ATOM
13293
OD1
ASP
B
295
49.649
10.115
−31.892
1.00
25.19
O


ATOM
13294
OD2
ASP
B
295
50.357
8.531
−30.525
1.00
30.29
O


ATOM
13295
C
ASP
B
295
45.636
9.087
−31.235
1.00
18.09
C


ATOM
13296
O
ASP
B
295
45.128
8.928
−32.340
1.00
19.33
O


ATOM
13298
N
ILE
B
296
45.079
8.649
−30.112
1.00
16.55
N


ATOM
13299
CA
ILE
B
296
43.816
7.925
−30.125
1.00
15.98
C


ATOM
13301
CB
ILE
B
296
43.411
7.463
−28.706
1.00
15.69
C


ATOM
13303
CG1
ILE
B
296
44.357
6.359
−28.231
1.00
16.99
C


ATOM
13306
CD1
ILE
B
296
44.121
5.906
−26.801
1.00
13.46
C


ATOM
13310
CG2
ILE
B
296
41.995
6.922
−28.701
1.00
14.84
C


ATOM
13314
C
ILE
B
296
42.700
8.764
−30.756
1.00
15.21
C


ATOM
13315
O
ILE
B
296
42.007
8.290
−31.653
1.00
14.11
O


ATOM
13317
N
TYR
B
297
42.546
9.999
−30.279
1.00
15.44
N


ATOM
13318
CA
TYR
B
297
41.499
10.921
−30.755
1.00
15.69
C


ATOM
13320
CB
TYR
B
297
41.322
12.098
−29.788
1.00
15.76
C


ATOM
13323
CG
TYR
B
297
40.545
11.798
−28.524
1.00
14.67
C


ATOM
13324
CD1
TYR
B
297
41.143
11.154
−27.454
1.00
16.18
C


ATOM
13326
CE1
TYR
B
297
40.443
10.898
−26.286
1.00
18.37
C


ATOM
13328
CZ
TYR
B
297
39.128
11.294
−26.178
1.00
19.71
C


ATOM
13329
OH
TYR
B
297
38.430
11.036
−25.022
1.00
20.24
O


ATOM
13331
CE2
TYR
B
297
38.512
11.943
−27.227
1.00
19.56
C


ATOM
13333
CD2
TYR
B
297
39.221
12.190
−28.391
1.00
15.92
C


ATOM
13335
C
TYR
B
297
41.807
11.484
−32.132
1.00
15.56
C


ATOM
13336
O
TYR
B
297
40.906
11.663
−32.943
1.00
17.25
O


ATOM
13338
N
ASP
B
298
43.080
11.761
−32.384
1.00
15.97
N


ATOM
13339
CA
ASP
B
298
43.518
12.312
−33.660
1.00
17.44
C


ATOM
13341
CB
ASP
B
298
44.999
12.733
−33.596
1.00
18.62
C


ATOM
13344
CG
ASP
B
298
45.455
13.513
−34.832
1.00
19.01
C


ATOM
13345
OD1
ASP
B
298
44.685
14.343
−35.345
1.00
25.00
O


ATOM
13346
OD2
ASP
B
298
46.599
13.311
−35.279
1.00
23.84
O


ATOM
13347
C
ASP
B
298
43.321
11.326
−34.804
1.00
16.87
C


ATOM
13348
O
ASP
B
298
42.796
11.709
−35.846
1.00
18.14
O


ATOM
13350
N
VAL
B
299
43.727
10.070
−34.609
1.00
15.93
N


ATOM
13351
CA
VAL
B
299
43.800
9.115
−35.721
1.00
16.58
C


ATOM
13353
CB
VAL
B
299
45.257
8.977
−36.235
1.00
17.12
C


ATOM
13355
CG1
VAL
B
299
45.771
10.325
−36.708
1.00
21.04
C


ATOM
13359
CG2
VAL
B
299
46.159
8.404
−35.161
1.00
15.17
C


ATOM
13363
C
VAL
B
299
43.254
7.699
−35.496
1.00
16.10
C


ATOM
13364
O
VAL
B
299
42.716
7.098
−36.436
1.00
15.92
O


ATOM
13366
N
TYR
B
300
43.394
7.138
−34.297
1.00
15.08
N


ATOM
13367
CA
TYR
B
300
43.108
5.712
−34.147
1.00
14.75
C


ATOM
13369
CB
TYR
B
300
43.954
5.070
−33.046
1.00
15.03
C


ATOM
13372
CG
TYR
B
300
43.879
3.561
−33.089
1.00
16.13
C


ATOM
13373
CD1
TYR
B
300
44.717
2.830
−33.921
1.00
17.83
C


ATOM
13375
CE1
TYR
B
300
44.642
1.440
−33.985
1.00
17.70
C


ATOM
13377
CZ
TYR
B
300
43.713
0.765
−33.216
1.00
17.51
C


ATOM
13378
OH
TYR
B
300
43.640
−0.613
−33.285
1.00
19.11
O


ATOM
13380
CE2
TYR
B
300
42.854
1.471
−32.391
1.00
18.38
C


ATOM
13382
CD2
TYR
B
300
42.940
2.867
−32.333
1.00
17.63
C


ATOM
13384
C
TYR
B
300
41.630
5.410
−33.915
1.00
13.88
C


ATOM
13385
O
TYR
B
300
41.070
4.529
−34.559
1.00
12.62
O


ATOM
13387
N
GLY
B
301
41.005
6.140
−33.000
1.00
14.29
N


ATOM
13388
CA
GLY
B
301
39.658
5.798
−32.539
1.00
14.39
C


ATOM
13391
C
GLY
B
301
38.506
6.381
−33.347
1.00
13.86
C


ATOM
13392
O
GLY
B
301
38.567
7.520
−33.803
1.00
13.36
O


ATOM
13394
N
THR
B
302
37.447
5.590
−33.495
1.00
13.78
N


ATOM
13395
CA
THR
B
302
36.217
6.035
−34.142
1.00
13.66
C


ATOM
13397
CB
THR
B
302
35.290
4.857
−34.477
1.00
13.51
C


ATOM
13399
OG1
THR
B
302
34.843
4.251
−33.254
1.00
16.25
O


ATOM
13401
CG2
THR
B
302
35.999
3.809
−35.355
1.00
7.50
C


ATOM
13405
C
THR
B
302
35.473
6.940
−33.180
1.00
14.64
C


ATOM
13406
O
THR
B
302
35.662
6.844
−31.966
1.00
15.85
O


ATOM
13408
N
LEU
B
303
34.608
7.798
−33.713
1.00
15.25
N


ATOM
13409
CA
LEU
B
303
33.882
8.773
−32.883
1.00
15.04
C


ATOM
13411
CB
LEU
B
303
32.968
9.647
−33.739
1.00
14.21
C


ATOM
13414
CG
LEU
B
303
32.494
10.948
−33.092
1.00
17.04
C


ATOM
13416
CD1
LEU
B
303
33.670
11.897
−32.842
1.00
12.42
C


ATOM
13420
CD2
LEU
B
303
31.429
11.617
−33.984
1.00
15.09
C


ATOM
13424
C
LEU
B
303
33.070
8.117
−31.766
1.00
15.41
C


ATOM
13425
O
LEU
B
303
32.981
8.669
−30.675
1.00
16.38
O


ATOM
13427
N
ASP
B
304
32.482
6.951
−32.036
1.00
16.29
N


ATOM
13428
CA
ASP
B
304
31.761
6.196
−31.003
1.00
17.01
C


ATOM
13430
CB
ASP
B
304
31.129
4.923
−31.574
1.00
17.38
C


ATOM
13433
CG
ASP
B
304
29.925
5.198
−32.452
1.00
19.47
C


ATOM
13434
OD1
ASP
B
304
29.499
6.370
−32.577
1.00
20.88
O


ATOM
13435
OD2
ASP
B
304
29.401
4.216
−33.023
1.00
20.92
O


ATOM
13436
C
ASP
B
304
32.706
5.800
−29.878
1.00
17.83
C


ATOM
13437
O
ASP
B
304
32.355
5.892
−28.694
1.00
18.36
O


ATOM
13439
N
GLU
B
305
33.898
5.341
−30.257
1.00
18.03
N


ATOM
13440
CA
GLU
B
305
34.901
4.905
−29.285
1.00
17.38
C


ATOM
13442
CB
GLU
B
305
36.086
4.230
−29.983
1.00
17.32
C


ATOM
13445
CG
GLU
B
305
35.729
2.871
−30.592
1.00
17.07
C


ATOM
13448
CD
GLU
B
305
36.903
2.187
−31.281
1.00
16.86
C


ATOM
13449
OE1
GLU
B
305
37.901
2.867
−31.610
1.00
11.15
O


ATOM
13450
OE2
GLU
B
305
36.815
0.956
−31.499
1.00
15.96
O


ATOM
13451
C
GLU
B
305
35.375
6.071
−28.437
1.00
17.24
C


ATOM
13452
O
GLU
B
305
35.583
5.917
−27.228
1.00
16.52
O


ATOM
13454
N
LEU
B
306
35.517
7.237
−29.069
1.00
17.03
N


ATOM
13455
CA
LEU
B
306
36.012
8.421
−28.374
1.00
17.64
C


ATOM
13457
CB
LEU
B
306
36.419
9.515
−29.374
1.00
17.28
C


ATOM
13460
CG
LEU
B
306
37.545
9.153
−30.359
1.00
16.34
C


ATOM
13462
CD1
LEU
B
306
37.842
10.288
−31.342
1.00
10.85
C


ATOM
13466
CD2
LEU
B
306
38.817
8.737
−29.624
1.00
14.09
C


ATOM
13470
C
LEU
B
306
34.987
8.941
−27.359
1.00
19.06
C


ATOM
13471
O
LEU
B
306
35.369
9.435
−26.296
1.00
19.45
O


ATOM
13473
N
GLU
B
307
33.697
8.818
−27.682
1.00
20.45
N


ATOM
13474
CA
GLU
B
307
32.620
9.204
−26.757
1.00
21.22
C


ATOM
13476
CB
GLU
B
307
31.245
9.134
−27.438
1.00
22.86
C


ATOM
13479
CG
GLU
B
307
30.811
10.407
−28.204
1.00
27.10
C


ATOM
13482
CD
GLU
B
307
30.340
11.554
−27.293
1.00
34.91
C


ATOM
13483
OE1
GLU
B
307
30.292
11.394
−26.049
1.00
38.72
O


ATOM
13484
OE2
GLU
B
307
30.021
12.638
−27.830
1.00
42.10
O


ATOM
13485
C
GLU
B
307
32.622
8.313
−25.519
1.00
20.59
C


ATOM
13486
O
GLU
B
307
32.476
8.796
−24.396
1.00
21.69
O


ATOM
13488
N
LEU
B
308
32.790
7.011
−25.719
1.00
19.50
N


ATOM
13489
CA
LEU
B
308
32.883
6.086
−24.594
1.00
19.04
C


ATOM
13491
CB
LEU
B
308
33.016
4.635
−25.079
1.00
19.13
C


ATOM
13494
CG
LEU
B
308
31.740
3.918
−25.533
1.00
18.67
C


ATOM
13496
CD1
LEU
B
308
32.073
2.546
−26.139
1.00
19.50
C


ATOM
13500
CD2
LEU
B
308
30.769
3.768
−24.374
1.00
17.20
C


ATOM
13504
C
LEU
B
308
34.059
6.430
−23.684
1.00
18.68
C


ATOM
13505
O
LEU
B
308
33.962
6.302
−22.469
1.00
19.37
O


ATOM
13507
N
PHE
B
309
35.172
6.859
−24.272
1.00
18.05
N


ATOM
13508
CA
PHE
B
309
36.386
7.110
−23.504
1.00
17.15
C


ATOM
13510
CB
PHE
B
309
37.605
7.172
−24.432
1.00
17.28
C


ATOM
13513
CG
PHE
B
309
38.920
7.113
−23.708
1.00
14.61
C


ATOM
13514
CD1
PHE
B
309
39.495
5.901
−23.396
1.00
12.78
C


ATOM
13516
CE1
PHE
B
309
40.698
5.844
−22.724
1.00
15.12
C


ATOM
13518
CZ
PHE
B
309
41.340
7.013
−22.355
1.00
12.95
C


ATOM
13520
CE2
PHE
B
309
40.772
8.222
−22.654
1.00
11.41
C


ATOM
13522
CD2
PHE
B
309
39.569
8.270
−23.328
1.00
12.83
C


ATOM
13524
C
PHE
B
309
36.245
8.404
−22.716
1.00
16.87
C


ATOM
13525
O
PHE
B
309
36.602
8.475
−21.549
1.00
16.52
O


ATOM
13527
N
THR
B
310
35.714
9.429
−23.368
1.00
17.61
N


ATOM
13528
CA
THR
B
310
35.441
10.686
−22.695
1.00
17.56
C


ATOM
13530
CB
THR
B
310
34.800
11.696
−23.631
1.00
16.99
C


ATOM
13532
OG1
THR
B
310
35.560
11.767
−24.843
1.00
15.50
O


ATOM
13534
CG2
THR
B
310
34.749
13.051
−22.964
1.00
15.81
C


ATOM
13538
C
THR
B
310
34.500
10.455
−21.526
1.00
18.58
C


ATOM
13539
O
THR
B
310
34.669
11.052
−20.462
1.00
20.74
O


ATOM
13541
N
ASP
B
311
33.518
9.580
−21.726
1.00
18.13
N


ATOM
13542
CA
ASP
B
311
32.553
9.275
−20.684
1.00
17.51
C


ATOM
13544
CB
ASP
B
311
31.429
8.389
−21.219
1.00
17.83
C


ATOM
13547
CG
ASP
B
311
30.258
8.314
−20.275
1.00
18.25
C


ATOM
13548
OD1
ASP
B
311
29.480
9.277
−20.208
1.00
26.72
O


ATOM
13549
OD2
ASP
B
311
30.105
7.288
−19.597
1.00
29.05
O


ATOM
13550
C
ASP
B
311
33.257
8.580
−19.540
1.00
16.69
C


ATOM
13551
O
ASP
B
311
33.103
8.976
−18.389
1.00
17.50
O


ATOM
13553
N
ALA
B
312
34.039
7.555
−19.867
1.00
15.64
N


ATOM
13554
CA
ALA
B
312
34.816
6.819
−18.871
1.00
15.21
C


ATOM
13556
CB
ALA
B
312
35.721
5.812
−19.540
1.00
14.61
C


ATOM
13560
C
ALA
B
312
35.645
7.756
−18.007
1.00
15.22
C


ATOM
13561
O
ALA
B
312
35.733
7.563
−16.798
1.00
16.10
O


ATOM
13563
N
VAL
B
313
36.246
8.770
−18.624
1.00
14.13
N


ATOM
13564
CA
VAL
B
313
37.075
9.700
−17.881
1.00
14.22
C


ATOM
13566
CB
VAL
B
313
37.978
10.538
−18.804
1.00
15.19
C


ATOM
13568
CG1
VAL
B
313
38.749
11.572
−17.997
1.00
11.03
C


ATOM
13572
CG2
VAL
B
313
38.945
9.627
−19.568
1.00
10.79
C


ATOM
13576
C
VAL
B
313
36.211
10.593
−16.998
1.00
15.13
C


ATOM
13577
O
VAL
B
313
36.531
10.796
−15.830
1.00
14.94
O


ATOM
13579
N
GLU
B
314
35.108
11.101
−17.547
1.00
16.20
N


ATOM
13580
CA
GLU
B
314
34.193
11.950
−16.781
1.00
16.05
C


ATOM
13582
CB
GLU
B
314
33.036
12.419
−17.653
1.00
16.20
C


ATOM
13585
CG
GLU
B
314
33.422
13.497
−18.652
1.00
18.20
C


ATOM
13588
CD
GLU
B
314
32.325
13.808
−19.654
1.00
21.40
C


ATOM
13589
OE1
GLU
B
314
31.197
13.296
−19.491
1.00
27.84
O


ATOM
13590
OE2
GLU
B
314
32.598
14.567
−20.610
1.00
23.02
O


ATOM
13591
C
GLU
B
314
33.652
11.243
−15.540
1.00
17.02
C


ATOM
13592
O
GLU
B
314
33.656
11.806
−14.444
1.00
17.47
O


ATOM
13594
N
ARG
B
315
33.198
10.008
−15.709
1.00
17.74
N


ATOM
13595
CA
ARG
B
315
32.628
9.249
−14.598
1.00
18.10
C


ATOM
13597
CB
ARG
B
315
31.817
8.066
−15.121
1.00
18.91
C


ATOM
13600
CG
ARG
B
315
30.589
8.473
−15.915
1.00
24.08
C


ATOM
13603
CD
ARG
B
315
29.633
7.310
−16.138
1.00
30.60
C


ATOM
13606
NE
ARG
B
315
30.214
6.283
−17.002
1.00
37.06
N


ATOM
13608
CZ
ARG
B
315
30.788
5.147
−16.593
1.00
43.47
C


ATOM
13609
NH1
ARG
B
315
30.881
4.833
−15.298
1.00
44.16
N


ATOM
13612
NH2
ARG
B
315
31.269
4.303
−17.505
1.00
45.37
N


ATOM
13615
C
ARG
B
315
33.685
8.755
−13.617
1.00
17.47
C


ATOM
13616
O
ARG
B
315
33.374
8.508
−12.457
1.00
18.44
O


ATOM
13618
N
TRP
B
316
34.923
8.609
−14.092
1.00
17.26
N


ATOM
13619
CA
TRP
B
316
36.069
8.177
−13.272
1.00
16.33
C


ATOM
13621
CB
TRP
B
316
36.580
9.323
−12.393
1.00
15.45
C


ATOM
13624
CG
TRP
B
316
38.014
9.122
−11.965
1.00
14.66
C


ATOM
13625
CD1
TRP
B
316
38.454
8.750
−10.734
1.00
14.27
C


ATOM
13627
NE1
TRP
B
316
39.818
8.647
−10.724
1.00
13.62
N


ATOM
13629
CE2
TRP
B
316
40.293
8.949
−11.970
1.00
14.25
C


ATOM
13630
CD2
TRP
B
316
39.182
9.247
−12.783
1.00
13.26
C


ATOM
13631
CE3
TRP
B
316
39.396
9.587
−14.123
1.00
15.54
C


ATOM
13633
CZ3
TRP
B
316
40.702
9.625
−14.598
1.00
16.04
C


ATOM
13635
CH2
TRP
B
316
41.788
9.321
−13.757
1.00
16.70
C


ATOM
13637
CZ2
TRP
B
316
41.603
8.980
−12.447
1.00
16.20
C


ATOM
13639
C
TRP
B
316
35.762
6.947
−12.423
1.00
16.99
C


ATOM
13640
O
TRP
B
316
36.080
6.881
−11.236
1.00
17.00
O


ATOM
13642
N
ASP
B
317
35.150
5.964
−13.058
1.00
18.49
N


ATOM
13643
CA
ASP
B
317
34.668
4.786
−12.365
1.00
19.92
C


ATOM
13645
CB
ASP
B
317
33.141
4.721
−12.514
1.00
19.90
C


ATOM
13648
CG
ASP
B
317
32.564
3.356
−12.237
1.00
18.95
C


ATOM
13649
OD1
ASP
B
317
32.961
2.692
−11.264
1.00
16.57
O


ATOM
13650
OD2
ASP
B
317
31.667
2.957
−13.002
1.00
28.92
O


ATOM
13651
C
ASP
B
317
35.381
3.591
−12.974
1.00
21.12
C


ATOM
13652
O
ASP
B
317
35.173
3.274
−14.137
1.00
21.51
O


ATOM
13654
N
VAL
B
318
36.256
2.960
−12.195
1.00
23.35
N


ATOM
13655
CA
VAL
B
318
36.988
1.787
−12.666
1.00
25.52
C


ATOM
13657
CB
VAL
B
318
38.007
1.290
−11.630
1.00
25.08
C


ATOM
13659
CG1
VAL
B
318
37.305
0.608
−10.476
1.00
23.89
C


ATOM
13663
CG2
VAL
B
318
39.001
0.351
−12.280
1.00
22.25
C


ATOM
13667
C
VAL
B
318
36.053
0.628
−13.015
1.00
28.28
C


ATOM
13668
O
VAL
B
318
36.313
−0.098
−13.967
1.00
26.89
O


ATOM
13670
N
ASN
B
319
34.958
0.487
−12.261
1.00
32.28
N


ATOM
13671
CA
ASN
B
319
34.049
−0.668
−12.379
1.00
35.37
C


ATOM
13673
CB
ASN
B
319
33.052
−0.731
−11.196
1.00
35.86
C


ATOM
13676
CG
ASN
B
319
33.746
−0.763
−9.810
1.00
40.22
C


ATOM
13677
OD1
ASN
B
319
34.413
−1.742
−9.450
1.00
42.18
O


ATOM
13678
ND2
ASN
B
319
33.557
0.304
−9.025
1.00
34.94
N


ATOM
13681
C
ASN
B
319
33.272
−0.690
−13.694
1.00
37.16
C


ATOM
13682
O
ASN
B
319
32.409
−1.539
−13.874
1.00
38.69
O


ATOM
13684
N
ALA
B
320
33.559
0.251
−14.594
1.00
38.94
N


ATOM
13685
CA
ALA
B
320
33.027
0.223
−15.957
1.00
40.53
C


ATOM
13687
CB
ALA
B
320
31.974
1.304
−16.140
1.00
41.10
C


ATOM
13691
C
ALA
B
320
34.164
0.383
−16.972
1.00
41.77
C


ATOM
13692
O
ALA
B
320
34.085
1.157
−17.926
1.00
42.42
O


ATOM
13694
N
ILE
B
321
35.236
−0.358
−16.729
1.00
42.81
N


ATOM
13695
CA
ILE
B
321
36.306
−0.547
−17.695
1.00
42.93
C


ATOM
13697
CB
ILE
B
321
37.560
−1.128
−16.988
1.00
43.23
C


ATOM
13699
CG1
ILE
B
321
38.804
−0.999
−17.852
1.00
44.69
C


ATOM
13702
CD1
ILE
B
321
40.018
−1.619
−17.201
1.00
45.95
C


ATOM
13706
CG2
ILE
B
321
37.354
−2.597
−16.591
1.00
44.13
C


ATOM
13710
C
ILE
B
321
35.798
−1.512
−18.773
1.00
43.65
C


ATOM
13711
O
ILE
B
321
36.136
−1.377
−19.949
1.00
45.47
O


ATOM
13713
N
ASN
B
322
34.946
−2.454
−18.355
1.00
42.07
N


ATOM
13714
CA
ASN
B
322
34.412
−3.510
−19.215
1.00
40.07
C


ATOM
13716
CB
ASN
B
322
33.561
−4.472
−18.377
1.00
41.01
C


ATOM
13719
CG
ASN
B
322
34.387
−5.295
−17.398
1.00
43.10
C


ATOM
13720
OD1
ASN
B
322
35.468
−5.800
−17.731
1.00
44.97
O


ATOM
13721
ND2
ASN
B
322
33.868
−5.451
−16.187
1.00
42.25
N


ATOM
13724
C
ASN
B
322
33.578
−3.069
−20.423
1.00
37.76
C


ATOM
13725
O
ASN
B
322
33.256
−3.897
−21.273
1.00
38.08
O


ATOM
13727
N
ASP
B
323
33.212
−1.794
−20.498
1.00
35.07
N


ATOM
13728
CA
ASP
B
323
32.456
−1.280
−21.642
1.00
33.38
C


ATOM
13730
CB
ASP
B
323
31.575
−0.091
−21.228
1.00
34.99
C


ATOM
13733
CG
ASP
B
323
30.816
−0.333
−19.927
1.00
38.70
C


ATOM
13734
OD1
ASP
B
323
30.318
−1.463
−19.723
1.00
44.24
O


ATOM
13735
OD2
ASP
B
323
30.715
0.616
−19.113
1.00
39.65
O


ATOM
13736
C
ASP
B
323
33.392
−0.832
−22.766
1.00
30.44
C


ATOM
13737
O
ASP
B
323
33.028
−0.877
−23.935
1.00
31.42
O


ATOM
13739
N
LEU
B
324
34.589
−0.383
−22.402
1.00
27.27
N


ATOM
13740
CA
LEU
B
324
35.561
0.136
−23.363
1.00
23.75
C


ATOM
13742
CB
LEU
B
324
36.727
0.808
−22.620
1.00
23.04
C


ATOM
13745
CG
LEU
B
324
36.451
2.117
−21.869
1.00
20.44
C


ATOM
13747
CD1
LEU
B
324
37.676
2.588
−21.089
1.00
16.65
C


ATOM
13751
CD2
LEU
B
324
36.016
3.192
−22.837
1.00
17.76
C


ATOM
13755
C
LEU
B
324
36.129
−0.956
−24.275
1.00
22.15
C


ATOM
13756
O
LEU
B
324
36.216
−2.123
−23.876
1.00
21.79
O


ATOM
13758
N
PRO
B
325
36.522
−0.581
−25.506
1.00
20.45
N


ATOM
13759
CA
PRO
B
325
37.323
−1.486
−26.334
1.00
19.14
C


ATOM
13761
CB
PRO
B
325
37.505
−0.725
−27.651
1.00
18.46
C


ATOM
13764
CG
PRO
B
325
36.926
0.614
−27.466
1.00
19.98
C


ATOM
13767
CD
PRO
B
325
36.088
0.621
−26.241
1.00
20.76
C


ATOM
13770
C
PRO
B
325
38.684
−1.791
−25.704
1.00
18.87
C


ATOM
13771
O
PRO
B
325
39.215
−0.973
−24.943
1.00
17.70
O


ATOM
13772
N
ASP
B
326
39.237
−2.958
−26.037
1.00
18.03
N


ATOM
13773
CA
ASP
B
326
40.427
−3.486
−25.368
1.00
17.58
C


ATOM
13775
CB
ASP
B
326
40.916
−4.767
−26.054
1.00
18.21
C


ATOM
13778
CG
ASP
B
326
39.932
−5.924
−25.922
1.00
17.05
C


ATOM
13779
OD1
ASP
B
326
38.906
−5.773
−25.224
1.00
18.68
O


ATOM
13780
OD2
ASP
B
326
40.188
−6.987
−26.520
1.00
14.18
O


ATOM
13781
C
ASP
B
326
41.570
−2.482
−25.293
1.00
17.83
C


ATOM
13782
O
ASP
B
326
42.147
−2.277
−24.219
1.00
18.93
O


ATOM
13784
N
TYR
B
327
41.899
−1.843
−26.413
1.00
16.28
N


ATOM
13785
CA
TYR
B
327
43.030
−0.919
−26.418
1.00
14.41
C


ATOM
13787
CB
TYR
B
327
43.369
−0.440
−27.839
1.00
14.21
C


ATOM
13790
CG
TYR
B
327
42.441
0.598
−28.434
1.00
12.82
C


ATOM
13791
CD1
TYR
B
327
41.266
0.232
−29.083
1.00
11.59
C


ATOM
13793
CE1
TYR
B
327
40.428
1.194
−29.629
1.00
14.08
C


ATOM
13795
CZ
TYR
B
327
40.773
2.536
−29.532
1.00
12.34
C


ATOM
13796
OH
TYR
B
327
39.965
3.529
−30.060
1.00
10.34
O


ATOM
13798
CE2
TYR
B
327
41.933
2.902
−28.894
1.00
10.85
C


ATOM
13800
CD2
TYR
B
327
42.755
1.946
−28.362
1.00
8.17
C


ATOM
13802
C
TYR
B
327
42.757
0.234
−25.460
1.00
14.32
C


ATOM
13803
O
TYR
B
327
43.667
0.699
−24.760
1.00
14.81
O


ATOM
13805
N
MET
B
328
41.495
0.661
−25.403
1.00
13.55
N


ATOM
13806
CA
MET
B
328
41.074
1.712
−24.481
1.00
14.08
C


ATOM
13808
CB
MET
B
328
39.728
2.298
−24.905
1.00
13.92
C


ATOM
13811
CG
MET
B
328
39.828
3.143
−26.140
1.00
14.37
C


ATOM
13814
SD
MET
B
328
38.290
3.955
−26.573
1.00
12.26
S


ATOM
13815
CE
MET
B
328
38.909
5.421
−27.417
1.00
13.00
C


ATOM
13819
C
MET
B
328
40.998
1.238
−23.030
1.00
14.20
C


ATOM
13820
O
MET
B
328
41.250
2.021
−22.115
1.00
14.05
O


ATOM
13822
N
LYS
B
329
40.643
−0.028
−22.821
1.00
15.10
N


ATOM
13823
CA
LYS
B
329
40.630
−0.606
−21.478
1.00
15.89
C


ATOM
13825
CB
LYS
B
329
40.363
−2.116
−21.532
1.00
17.28
C


ATOM
13828
CG
LYS
B
329
38.896
−2.473
−21.634
1.00
20.84
C


ATOM
13831
CD
LYS
B
329
38.664
−3.931
−21.969
1.00
24.16
C


ATOM
13834
CE
LYS
B
329
37.232
−4.336
−21.638
1.00
27.79
C


ATOM
13837
NZ
LYS
B
329
36.674
−5.298
−22.613
1.00
26.15
N


ATOM
13841
C
LYS
B
329
41.963
−0.348
−20.806
1.00
15.46
C


ATOM
13842
O
LYS
B
329
42.024
0.235
−19.729
1.00
15.21
O


ATOM
13844
N
LEU
B
330
43.027
−0.762
−21.484
1.00
15.37
N


ATOM
13845
CA
LEU
B
330
44.385
−0.697
−20.954
1.00
15.24
C


ATOM
13847
CB
LEU
B
330
45.323
−1.404
−21.933
1.00
15.47
C


ATOM
13850
CG
LEU
B
330
46.642
−1.982
−21.444
1.00
16.80
C


ATOM
13852
CD1
LEU
B
330
46.427
−3.085
−20.410
1.00
16.57
C


ATOM
13856
CD2
LEU
B
330
47.406
−2.511
−22.654
1.00
18.67
C


ATOM
13860
C
LEU
B
330
44.818
0.758
−20.733
1.00
15.71
C


ATOM
13861
O
LEU
B
330
45.311
1.124
−19.657
1.00
16.15
O


ATOM
13863
N
ACYS
B
331
44.615
1.583
−21.754
0.50
15.25
N


ATOM
13864
N
BCYS
B
331
44.628
1.583
−21.760
0.50
15.88
N


ATOM
13865
CA
ACYS
B
331
44.936
3.001
−21.681
0.50
14.26
C


ATOM
13866
CA
BCYS
B
331
44.911
3.012
−21.675
0.50
15.48
C


ATOM
13869
CB
ACYS
B
331
44.556
3.683
−22.995
0.50
14.04
C


ATOM
13870
CB
BCYS
B
331
44.424
3.717
−22.936
0.50
15.49
C


ATOM
13875
SG
ACYS
B
331
45.108
5.391
−23.131
0.50
11.76
S


ATOM
13876
SG
BCYS
B
331
45.579
3.645
−24.291
0.50
17.45
S


ATOM
13879
C
ACYS
B
331
44.225
3.684
−20.510
0.50
14.40
C


ATOM
13880
C
BCYS
B
331
44.232
3.650
−20.476
0.50
15.03
C


ATOM
13881
O
ACYS
B
331
44.838
4.461
−19.774
0.50
14.06
O


ATOM
13882
O
BCYS
B
331
44.867
4.363
−19.697
0.50
14.71
O


ATOM
13885
N
PHE
B
332
42.936
3.393
−20.338
1.00
14.55
N


ATOM
13886
CA
PHE
B
332
42.161
3.984
−19.255
1.00
15.52
C


ATOM
13888
CB
PHE
B
332
40.673
3.660
−19.386
1.00
16.28
C


ATOM
13891
CG
PHE
B
332
39.845
4.208
−18.256
1.00
19.29
C


ATOM
13892
CD1
PHE
B
332
39.407
5.523
−18.274
1.00
19.62
C


ATOM
13894
CE1
PHE
B
332
38.655
6.031
−17.227
1.00
18.67
C


ATOM
13896
CZ
PHE
B
332
38.353
5.231
−16.142
1.00
16.97
C


ATOM
13898
CE2
PHE
B
332
38.790
3.931
−16.108
1.00
18.43
C


ATOM
13900
CD2
PHE
B
332
39.536
3.421
−17.156
1.00
20.41
C


ATOM
13902
C
PHE
B
332
42.675
3.538
−17.885
1.00
14.48
C


ATOM
13903
O
PHE
B
332
42.924
4.357
−17.013
1.00
14.07
O


ATOM
13905
N
LEU
B
333
42.860
2.239
−17.711
1.00
14.61
N


ATOM
13906
CA
LEU
B
333
43.276
1.720
−16.418
1.00
14.22
C


ATOM
13908
CB
LEU
B
333
43.239
0.185
−16.400
1.00
13.21
C


ATOM
13911
CG
LEU
B
333
43.521
−0.459
−15.040
1.00
13.37
C


ATOM
13913
CD1
LEU
B
333
42.680
0.164
−13.930
1.00
10.22
C


ATOM
13917
CD2
LEU
B
333
43.293
−1.955
−15.103
1.00
8.11
C


ATOM
13921
C
LEU
B
333
44.656
2.240
−16.036
1.00
14.28
C


ATOM
13922
O
LEU
B
333
44.897
2.528
−14.871
1.00
15.54
O


ATOM
13924
N
ALA
B
334
45.550
2.371
−17.014
1.00
13.82
N


ATOM
13925
CA
ALA
B
334
46.874
2.952
−16.769
1.00
13.62
C


ATOM
13927
CB
ALA
B
334
47.718
2.889
−18.025
1.00
13.34
C


ATOM
13931
C
ALA
B
334
46.777
4.393
−16.288
1.00
14.17
C


ATOM
13932
O
ALA
B
334
47.452
4.779
−15.338
1.00
15.62
O


ATOM
13934
N
LEU
B
335
45.947
5.184
−16.963
1.00
14.35
N


ATOM
13935
CA
LEU
B
335
45.723
6.580
−16.607
1.00
14.33
C


ATOM
13937
CB
LEU
B
335
44.861
7.256
−17.682
1.00
15.56
C


ATOM
13940
CG
LEU
B
335
44.541
8.748
−17.512
1.00
17.65
C


ATOM
13942
CD1
LEU
B
335
45.787
9.613
−17.669
1.00
14.16
C


ATOM
13946
CD2
LEU
B
335
43.447
9.172
−18.495
1.00
14.60
C


ATOM
13950
C
LEU
B
335
45.037
6.665
−15.248
1.00
13.80
C


ATOM
13951
O
LEU
B
335
45.472
7.396
−14.372
1.00
13.76
O


ATOM
13953
N
TYR
B
336
43.973
5.886
−15.082
1.00
13.24
N


ATOM
13954
CA
TYR
B
336
43.235
5.812
−13.826
1.00
12.46
C


ATOM
13956
CB
TYR
B
336
42.188
4.706
−13.911
1.00
12.60
C


ATOM
13959
CG
TYR
B
336
41.174
4.701
−12.790
1.00
14.72
C


ATOM
13960
CD1
TYR
B
336
40.079
5.566
−12.810
1.00
16.98
C


ATOM
13962
CE1
TYR
B
336
39.133
5.553
−11.793
1.00
13.34
C


ATOM
13964
CZ
TYR
B
336
39.274
4.670
−10.743
1.00
16.89
C


ATOM
13965
OH
TYR
B
336
38.348
4.649
−9.729
1.00
18.69
O


ATOM
13967
CE2
TYR
B
336
40.345
3.798
−10.704
1.00
17.64
C


ATOM
13969
CD2
TYR
B
336
41.286
3.814
−11.730
1.00
13.75
C


ATOM
13971
C
TYR
B
336
44.158
5.562
−12.636
1.00
12.04
C


ATOM
13972
O
TYR
B
336
44.120
6.296
−11.652
1.00
12.01
O


ATOM
13974
N
ASN
B
337
44.995
4.534
−12.730
1.00
11.13
N


ATOM
13975
CA
ASN
B
337
45.918
4.212
−11.646
1.00
10.21
C


ATOM
13977
CB
ASN
B
337
46.635
2.889
−11.913
1.00
9.74
C


ATOM
13980
CG
ASN
B
337
45.706
1.693
−11.840
1.00
10.79
C


ATOM
13981
OD1
ASN
B
337
44.601
1.791
−11.311
1.00
16.13
O


ATOM
13982
ND2
ASN
B
337
46.153
0.550
−12.369
1.00
8.28
N


ATOM
13985
C
ASN
B
337
46.937
5.319
−11.415
1.00
10.08
C


ATOM
13986
O
ASN
B
337
47.159
5.728
−10.282
1.00
11.29
O


ATOM
13988
N
THR
B
338
47.547
5.803
−12.491
1.00
10.00
N


ATOM
13989
CA
THR
B
338
48.571
6.848
−12.403
1.00
10.09
C


ATOM
13991
CB
THR
B
338
49.077
7.253
−13.809
1.00
10.88
C


ATOM
13993
OG1
THR
B
338
49.627
6.102
−14.465
1.00
10.50
O


ATOM
13995
CG2
THR
B
338
50.137
8.365
−13.727
1.00
5.80
C


ATOM
13999
C
THR
B
338
48.041
8.089
−11.694
1.00
10.55
C


ATOM
14000
O
THR
B
338
48.674
8.592
−10.757
1.00
10.03
O


ATOM
14002
N
ILE
B
339
46.881
8.574
−12.140
1.00
10.19
N


ATOM
14003
CA
ILE
B
339
46.253
9.735
−11.514
1.00
10.96
C


ATOM
14005
CB
ILE
B
339
45.007
10.233
−12.286
1.00
10.74
C


ATOM
14007
CG1
ILE
B
339
45.343
10.556
−13.754
1.00
10.45
C


ATOM
14010
CD1
ILE
B
339
46.679
11.224
−13.953
1.00
10.85
C


ATOM
14014
CG2
ILE
B
339
44.436
11.465
−11.613
1.00
9.41
C


ATOM
14018
C
ILE
B
339
45.893
9.478
−10.035
1.00
11.92
C


ATOM
14019
O
ILE
B
339
46.194
10.313
−9.173
1.00
13.70
O


ATOM
14021
N
ASN
B
340
45.286
8.335
−9.725
1.00
10.85
N


ATOM
14022
CA
ASN
B
340
44.963
8.024
−8.330
1.00
10.25
C


ATOM
14024
CB
ASN
B
340
44.146
6.739
−8.203
1.00
9.53
C


ATOM
14027
CG
ASN
B
340
42.775
6.845
−8.830
1.00
12.20
C


ATOM
14028
OD1
ASN
B
340
42.251
7.943
−9.039
1.00
13.10
O


ATOM
14029
ND2
ASN
B
340
42.186
5.694
−9.153
1.00
13.08
N


ATOM
14032
C
ASN
B
340
46.222
7.902
−7.485
1.00
11.03
C


ATOM
14033
O
ASN
B
340
46.181
8.110
−6.269
1.00
11.05
O


ATOM
14035
N
GLU
B
341
47.335
7.543
−8.124
1.00
11.88
N


ATOM
14036
CA
GLU
B
341
48.619
7.433
−7.434
1.00
12.69
C


ATOM
14038
CB
GLU
B
341
49.650
6.745
−8.329
1.00
14.26
C


ATOM
14041
CG
GLU
B
341
50.972
6.373
−7.653
1.00
24.71
C


ATOM
14044
CD
GLU
B
341
51.950
5.703
−8.617
1.00
38.29
C


ATOM
14045
OE1
GLU
B
341
53.119
6.149
−8.686
1.00
46.78
O


ATOM
14046
OE2
GLU
B
341
51.546
4.743
−9.320
1.00
46.75
O


ATOM
14047
C
GLU
B
341
49.117
8.809
−7.020
1.00
11.22
C


ATOM
14048
O
GLU
B
341
49.564
8.989
−5.898
1.00
10.21
O


ATOM
14050
N
ILE
B
342
49.031
9.773
−7.934
1.00
11.58
N


ATOM
14051
CA
ILE
B
342
49.442
11.145
−7.658
1.00
11.56
C


ATOM
14053
CB
ILE
B
342
49.443
12.001
−8.949
1.00
12.11
C


ATOM
14055
CG1
ILE
B
342
50.595
11.582
−9.860
1.00
13.42
C


ATOM
14058
CD1
ILE
B
342
50.452
12.080
−11.287
1.00
12.74
C


ATOM
14062
CG2
ILE
B
342
49.575
13.481
−8.646
1.00
7.54
C


ATOM
14066
C
ILE
B
342
48.532
11.760
−6.591
1.00
12.57
C


ATOM
14067
O
ILE
B
342
48.996
12.505
−5.734
1.00
14.17
O


ATOM
14069
N
ALA
B
343
47.243
11.437
−6.635
1.00
12.30
N


ATOM
14070
CA
ALA
B
343
46.301
11.910
−5.623
1.00
12.11
C


ATOM
14072
CB
ALA
B
343
44.881
11.496
−5.981
1.00
11.62
C


ATOM
14076
C
ALA
B
343
46.688
11.380
−4.243
1.00
12.90
C


ATOM
14077
O
ALA
B
343
46.656
12.119
−3.248
1.00
14.47
O


ATOM
14079
N
TYR
B
344
47.073
10.104
−4.196
1.00
12.86
N


ATOM
14080
CA
TYR
B
344
47.562
9.480
−2.964
1.00
11.90
C


ATOM
14082
CB
TYR
B
344
47.878
7.989
−3.165
1.00
11.32
C


ATOM
14085
CG
TYR
B
344
48.448
7.352
−1.923
1.00
5.70
C


ATOM
14086
CD1
TYR
B
344
47.614
6.860
−0.928
1.00
4.60
C


ATOM
14088
CE1
TYR
B
344
48.138
6.301
0.228
1.00
4.71
C


ATOM
14090
CZ
TYR
B
344
49.504
6.244
0.395
1.00
2.00
C


ATOM
14091
OH
TYR
B
344
50.028
5.692
1.532
1.00
6.52
O


ATOM
14093
CE2
TYR
B
344
50.345
6.732
−0.572
1.00
2.00
C


ATOM
14095
CD2
TYR
B
344
49.819
7.287
−1.719
1.00
2.87
C


ATOM
14097
C
TYR
B
344
48.798
10.184
−2.422
1.00
12.46
C


ATOM
14098
O
TYR
B
344
48.893
10.413
−1.224
1.00
12.96
O


ATOM
14100
N
ASP
B
345
49.747
10.509
−3.296
1.00
13.52
N


ATOM
14101
CA
ASP
B
345
50.954
11.230
−2.878
1.00
14.39
C


ATOM
14103
CB
ASP
B
345
51.839
11.583
−4.082
1.00
14.28
C


ATOM
14106
CG
ASP
B
345
52.569
10.380
−4.659
1.00
17.59
C


ATOM
14107
OD1
ASP
B
345
52.720
9.361
−3.951
1.00
21.20
O


ATOM
14108
OD2
ASP
B
345
53.009
10.466
−5.830
1.00
24.42
O


ATOM
14109
C
ASP
B
345
50.592
12.511
−2.138
1.00
14.27
C


ATOM
14110
O
ASP
B
345
51.136
12.784
−1.079
1.00
13.47
O


ATOM
14112
N
ASN
B
346
49.672
13.292
−2.705
1.00
15.07
N


ATOM
14113
CA
ASN
B
346
49.266
14.562
−2.099
1.00
15.37
C


ATOM
14115
CB
ASN
B
346
48.500
15.430
−3.100
1.00
15.43
C


ATOM
14118
CG
ASN
B
346
49.413
16.064
−4.114
1.00
16.36
C


ATOM
14119
OD1
ASN
B
346
49.869
17.194
−3.929
1.00
19.33
O


ATOM
14120
ND2
ASN
B
346
49.721
15.330
−5.174
1.00
15.63
N


ATOM
14123
C
ASN
B
346
48.453
14.376
−0.826
1.00
15.35
C


ATOM
14124
O
ASN
B
346
48.601
15.141
0.128
1.00
14.31
O


ATOM
14126
N
LEU
B
347
47.601
13.355
−0.801
1.00
15.31
N


ATOM
14127
CA
LEU
B
347
46.898
13.026
0.428
1.00
14.92
C


ATOM
14129
CB
LEU
B
347
45.934
11.868
0.205
1.00
14.76
C


ATOM
14132
CG
LEU
B
347
45.049
11.490
1.398
1.00
16.42
C


ATOM
14134
CD1
LEU
B
347
44.343
12.715
1.979
1.00
14.04
C


ATOM
14138
CD2
LEU
B
347
44.045
10.416
0.999
1.00
10.08
C


ATOM
14142
C
LEU
B
347
47.926
12.708
1.519
1.00
15.54
C


ATOM
14143
O
LEU
B
347
47.884
13.278
2.601
1.00
17.24
O


ATOM
14145
N
LYS
B
348
48.881
11.840
1.211
1.00
15.74
N


ATOM
14146
CA
LYS
B
348
49.935
11.467
2.158
1.00
16.65
C


ATOM
14148
CB
LYS
B
348
50.886
10.468
1.490
1.00
16.49
C


ATOM
14151
CG
LYS
B
348
51.943
9.856
2.391
1.00
17.97
C


ATOM
14154
CD
LYS
B
348
52.819
8.851
1.621
1.00
21.19
C


ATOM
14157
CE
LYS
B
348
54.204
9.399
1.256
1.00
22.84
C


ATOM
14160
NZ
LYS
B
348
54.260
10.176
−0.004
1.00
24.37
N


ATOM
14164
C
LYS
B
348
50.734
12.673
2.657
1.00
17.77
C


ATOM
14165
O
LYS
B
348
50.910
12.866
3.862
1.00
18.58
O


ATOM
14167
N
ASP
B
349
51.221
13.479
1.724
1.00
18.87
N


ATOM
14168
CA
ASP
B
349
52.179
14.534
2.044
1.00
19.87
C


ATOM
14170
CB
ASP
B
349
53.155
14.718
0.879
1.00
20.01
C


ATOM
14173
CG
ASP
B
349
54.010
13.479
0.633
1.00
21.35
C


ATOM
14174
OD1
ASP
B
349
54.069
12.608
1.523
1.00
23.56
O


ATOM
14175
OD2
ASP
B
349
54.626
13.377
−0.447
1.00
25.12
O


ATOM
14176
C
ASP
B
349
51.544
15.871
2.420
1.00
19.82
C


ATOM
14177
O
ASP
B
349
52.149
16.645
3.139
1.00
21.01
O


ATOM
14179
N
LYS
B
350
50.336
16.143
1.950
1.00
20.14
N


ATOM
14180
CA
LYS
B
350
49.688
17.424
2.227
1.00
20.29
C


ATOM
14182
CB
LYS
B
350
49.479
18.190
0.923
1.00
20.04
C


ATOM
14185
CG
LYS
B
350
50.760
18.489
0.170
1.00
23.22
C


ATOM
14188
CD
LYS
B
350
50.433
19.125
−1.168
1.00
31.38
C


ATOM
14191
CE
LYS
B
350
51.681
19.508
−1.954
1.00
32.47
C


ATOM
14194
NZ
LYS
B
350
51.313
19.744
−3.370
1.00
29.78
N


ATOM
14198
C
LYS
B
350
48.356
17.310
2.972
1.00
20.08
C


ATOM
14199
O
LYS
B
350
47.831
18.315
3.441
1.00
21.09
O


ATOM
14201
N
GLY
B
351
47.806
16.104
3.081
1.00
19.11
N


ATOM
14202
CA
GLY
B
351
46.551
15.899
3.797
1.00
18.35
C


ATOM
14205
C
GLY
B
351
45.336
16.452
3.080
1.00
18.36
C


ATOM
14206
O
GLY
B
351
44.336
16.763
3.714
1.00
18.14
O


ATOM
14208
N
GLU
B
352
45.423
16.566
1.757
1.00
19.29
N


ATOM
14209
CA
GLU
B
352
44.339
17.104
0.939
1.00
20.17
C


ATOM
14211
CB
GLU
B
352
44.776
18.404
0.251
1.00
20.76
C


ATOM
14214
CG
GLU
B
352
45.102
19.567
1.196
1.00
23.95
C


ATOM
14217
CD
GLU
B
352
43.875
20.161
1.878
1.00
28.83
C


ATOM
14218
OE1
GLU
B
352
42.754
20.032
1.333
1.00
28.49
O


ATOM
14219
OE2
GLU
B
352
44.040
20.762
2.966
1.00
35.13
O


ATOM
14220
C
GLU
B
352
43.943
16.097
−0.130
1.00
20.14
C


ATOM
14221
O
GLU
B
352
44.802
15.409
−0.685
1.00
20.62
O


ATOM
14223
N
ASN
B
353
42.641
16.012
−0.404
1.00
19.53
N


ATOM
14224
CA
ASN
B
353
42.140
15.254
−1.532
1.00
18.31
C


ATOM
14226
CB
ASN
B
353
40.779
14.641
−1.236
1.00
18.23
C


ATOM
14229
CG
ASN
B
353
40.241
13.851
−2.414
1.00
19.94
C


ATOM
14230
OD1
ASN
B
353
40.927
13.679
−3.422
1.00
18.91
O


ATOM
14231
ND2
ASN
B
353
39.017
13.364
−2.293
1.00
23.60
N


ATOM
14234
C
ASN
B
353
42.023
16.150
−2.751
1.00
17.97
C


ATOM
14235
O
ASN
B
353
41.043
16.877
−2.896
1.00
19.13
O


ATOM
14237
N
ILE
B
354
43.012
16.072
−3.634
1.00
16.21
N


ATOM
14238
CA
ILE
B
354
43.015
16.859
−4.852
1.00
14.55
C


ATOM
14240
CB
ILE
B
354
44.415
17.426
−5.129
1.00
15.27
C


ATOM
14242
CG1
ILE
B
354
45.388
16.315
−5.545
1.00
15.94
C


ATOM
14245
CD1
ILE
B
354
46.671
16.828
−6.126
1.00
19.64
C


ATOM
14249
CG2
ILE
B
354
44.930
18.140
−3.902
1.00
12.01
C


ATOM
14253
C
ILE
B
354
42.554
16.066
−6.072
1.00
14.06
C


ATOM
14254
O
ILE
B
354
42.687
16.536
−7.208
1.00
12.64
O


ATOM
14256
N
LEU
B
355
42.007
14.871
−5.846
1.00
13.89
N


ATOM
14257
CA
LEU
B
355
41.636
13.996
−6.955
1.00
14.02
C


ATOM
14259
CB
LEU
B
355
41.099
12.641
−6.464
1.00
13.01
C


ATOM
14262
CG
LEU
B
355
40.891
11.570
−7.552
1.00
12.70
C


ATOM
14264
CD1
LEU
B
355
42.184
11.255
−8.292
1.00
8.91
C


ATOM
14268
CD2
LEU
B
355
40.328
10.293
−6.959
1.00
11.46
C


ATOM
14272
C
LEU
B
355
40.629
14.677
−7.881
1.00
15.15
C


ATOM
14273
O
LEU
B
355
40.824
14.687
−9.103
1.00
15.76
O


ATOM
14275
N
PRO
B
356
39.567
15.277
−7.310
1.00
15.69
N


ATOM
14276
CA
PRO
B
356
38.588
15.911
−8.193
1.00
15.84
C


ATOM
14278
CB
PRO
B
356
37.643
16.626
−7.216
1.00
15.92
C


ATOM
14281
CG
PRO
B
356
37.785
15.879
−5.934
1.00
14.63
C


ATOM
14284
CD
PRO
B
356
39.215
15.450
−5.885
1.00
14.65
C


ATOM
14287
C
PRO
B
356
39.213
16.906
−9.175
1.00
15.91
C


ATOM
14288
O
PRO
B
356
38.775
16.986
−10.320
1.00
17.51
O


ATOM
14289
N
TYR
B
357
40.236
17.636
−8.743
1.00
15.73
N


ATOM
14290
CA
TYR
B
357
40.881
18.631
−9.610
1.00
16.23
C


ATOM
14292
CB
TYR
B
357
41.723
19.615
−8.784
1.00
15.93
C


ATOM
14295
CG
TYR
B
357
40.999
20.092
−7.540
1.00
20.66
C


ATOM
14296
CD1
TYR
B
357
39.804
20.803
−7.633
1.00
26.03
C


ATOM
14298
CE1
TYR
B
357
39.118
21.225
−6.495
1.00
23.97
C


ATOM
14300
CZ
TYR
B
357
39.627
20.933
−5.252
1.00
27.38
C


ATOM
14301
OH
TYR
B
357
38.962
21.350
−4.118
1.00
32.89
O


ATOM
14303
CE2
TYR
B
357
40.808
20.220
−5.134
1.00
26.43
C


ATOM
14305
CD2
TYR
B
357
41.482
19.801
−6.276
1.00
24.51
C


ATOM
14307
C
TYR
B
357
41.711
17.973
−10.721
1.00
16.38
C


ATOM
14308
O
TYR
B
357
41.704
18.441
−11.867
1.00
17.20
O


ATOM
14310
N
LEU
B
358
42.396
16.877
−10.398
1.00
16.21
N


ATOM
14311
CA
LEU
B
358
43.185
16.150
−11.395
1.00
15.98
C


ATOM
14313
CB
LEU
B
358
44.086
15.105
−10.725
1.00
16.16
C


ATOM
14316
CG
LEU
B
358
45.086
15.561
−9.655
1.00
15.44
C


ATOM
14318
CD1
LEU
B
358
45.836
14.356
−9.073
1.00
9.30
C


ATOM
14322
CD2
LEU
B
358
46.066
16.601
−10.202
1.00
12.13
C


ATOM
14326
C
LEU
B
358
42.278
15.480
−12.440
1.00
15.92
C


ATOM
14327
O
LEU
B
358
42.495
15.614
−13.639
1.00
14.71
O


ATOM
14329
N
THR
B
359
41.252
14.777
−11.974
1.00
16.60
N


ATOM
14330
CA
THR
B
359
40.301
14.127
−12.862
1.00
17.22
C


ATOM
14332
CB
THR
B
359
39.284
13.291
−12.084
1.00
16.72
C


ATOM
14334
OG1
THR
B
359
38.617
14.125
−11.138
1.00
17.96
O


ATOM
14336
CG2
THR
B
359
39.972
12.154
−11.355
1.00
17.03
C


ATOM
14340
C
THR
B
359
39.525
15.119
−13.722
1.00
19.12
C


ATOM
14341
O
THR
B
359
39.177
14.806
−14.863
1.00
20.95
O


ATOM
14343
N
LYS
B
360
39.233
16.301
−13.183
1.00
20.20
N


ATOM
14344
CA
LYS
B
360
38.542
17.340
−13.958
1.00
20.41
C


ATOM
14346
CB
LYS
B
360
38.157
18.526
−13.080
1.00
20.61
C


ATOM
14349
CG
LYS
B
360
37.581
19.736
−13.839
1.00
24.40
C


ATOM
14352
CD
LYS
B
360
36.233
19.445
−14.489
1.00
28.55
C


ATOM
14355
CE
LYS
B
360
35.611
20.716
−15.054
1.00
30.13
C


ATOM
14358
NZ
LYS
B
360
34.583
20.436
−16.094
1.00
29.00
N


ATOM
14362
C
LYS
B
360
39.410
17.819
−15.108
1.00
20.46
C


ATOM
14363
O
LYS
B
360
38.913
18.001
−16.215
1.00
22.09
O


ATOM
14365
N
ALA
B
361
40.701
18.025
−14.845
1.00
19.21
N


ATOM
14366
CA
ALA
B
361
41.618
18.479
−15.885
1.00
18.03
C


ATOM
14368
CB
ALA
B
361
43.014
18.667
−15.333
1.00
17.16
C


ATOM
14372
C
ALA
B
361
41.633
17.499
−17.048
1.00
17.75
C


ATOM
14373
O
ALA
B
361
41.536
17.907
−18.206
1.00
19.49
O


ATOM
14375
N
TRP
B
362
41.739
16.210
−16.735
1.00
16.54
N


ATOM
14376
CA
TRP
B
362
41.714
15.161
−17.760
1.00
16.29
C


ATOM
14378
CB
TRP
B
362
42.037
13.796
−17.153
1.00
15.85
C


ATOM
14381
CG
TRP
B
362
43.484
13.580
−17.128
1.00
14.86
C


ATOM
14382
CD1
TRP
B
362
44.306
13.657
−16.053
1.00
11.87
C


ATOM
14384
NE1
TRP
B
362
45.605
13.435
−16.440
1.00
15.32
N


ATOM
14386
CE2
TRP
B
362
45.630
13.213
−17.792
1.00
15.07
C


ATOM
14387
CD2
TRP
B
362
44.312
13.306
−18.257
1.00
12.81
C


ATOM
14388
CE3
TRP
B
362
44.060
13.119
−19.614
1.00
13.50
C


ATOM
14390
CZ3
TRP
B
362
45.118
12.849
−20.453
1.00
13.63
C


ATOM
14392
CH2
TRP
B
362
46.421
12.761
−19.967
1.00
14.21
C


ATOM
14394
CZ2
TRP
B
362
46.700
12.938
−18.640
1.00
16.67
C


ATOM
14396
C
TRP
B
362
40.406
15.085
−18.541
1.00
16.06
C


ATOM
14397
O
TRP
B
362
40.426
14.901
−19.760
1.00
16.19
O


ATOM
14399
N
ALA
B
363
39.283
15.224
−17.840
1.00
15.51
N


ATOM
14400
CA
ALA
B
363
37.974
15.244
−18.485
1.00
15.12
C


ATOM
14402
CB
ALA
B
363
36.854
15.331
−17.452
1.00
13.50
C


ATOM
14406
C
ALA
B
363
37.895
16.413
−19.465
1.00
15.66
C


ATOM
14407
O
ALA
B
363
37.372
16.255
−20.575
1.00
15.88
O


ATOM
14409
N
ASP
B
364
38.427
17.568
−19.063
1.00
14.27
N


ATOM
14410
CA
ASP
B
364
38.424
18.748
−19.923
1.00
15.12
C


ATOM
14412
CB
ASP
B
364
38.874
20.003
−19.162
1.00
15.25
C


ATOM
14415
CG
ASP
B
364
37.838
20.497
−18.168
1.00
15.91
C


ATOM
14416
OD1
ASP
B
364
36.677
20.034
−18.198
1.00
20.98
O


ATOM
14417
OD2
ASP
B
364
38.197
21.346
−17.336
1.00
22.28
O


ATOM
14418
C
ASP
B
364
39.311
18.564
−21.143
1.00
15.25
C


ATOM
14419
O
ASP
B
364
38.992
19.056
−22.220
1.00
16.41
O


ATOM
14421
N
LEU
B
365
40.428
17.868
−20.977
1.00
16.10
N


ATOM
14422
CA
LEU
B
365
41.332
17.656
−22.086
1.00
16.42
C


ATOM
14424
CB
LEU
B
365
42.672
17.104
−21.607
1.00
15.96
C


ATOM
14427
CG
LEU
B
365
43.677
16.852
−22.738
1.00
15.86
C


ATOM
14429
CD1
LEU
B
365
43.892
18.104
−23.536
1.00
11.79
C


ATOM
14433
CD2
LEU
B
365
44.997
16.314
−22.210
1.00
16.31
C


ATOM
14437
C
LEU
B
365
40.673
16.705
−23.077
1.00
17.01
C


ATOM
14438
O
LEU
B
365
40.628
16.969
−24.282
1.00
17.06
O


ATOM
14440
N
CYS
B
366
40.143
15.607
−22.559
1.00
17.19
N


ATOM
14441
CA
CYS
B
366
39.452
14.646
−23.400
1.00
17.88
C


ATOM
14443
CB
CYS
B
366
39.016
13.438
−22.574
1.00
18.28
C


ATOM
14446
SG
CYS
B
366
40.448
12.465
−22.017
1.00
19.51
S


ATOM
14448
C
CYS
B
366
38.278
15.288
−24.131
1.00
16.95
C


ATOM
14449
O
CYS
B
366
38.055
15.001
−25.300
1.00
17.64
O


ATOM
14451
N
ASN
B
367
37.559
16.186
−23.463
1.00
17.32
N


ATOM
14452
CA
ASN
B
367
36.461
16.913
−24.115
1.00
16.82
C


ATOM
14454
CB
ASN
B
367
35.572
17.626
−23.099
1.00
14.42
C


ATOM
14457
CG
ASN
B
367
34.459
16.719
−22.571
1.00
16.19
C


ATOM
14458
OD1
ASN
B
367
33.560
16.329
−23.313
1.00
19.75
O


ATOM
14459
ND2
ASN
B
367
34.529
16.366
−21.294
1.00
16.43
N


ATOM
14462
C
ASN
B
367
36.930
17.866
−25.208
1.00
17.41
C


ATOM
14463
O
ASN
B
367
36.222
18.077
−26.185
1.00
19.06
O


ATOM
14465
N
ALA
B
368
38.124
18.428
−25.058
1.00
17.51
N


ATOM
14466
CA
ALA
B
368
38.687
19.283
−26.095
1.00
16.21
C


ATOM
14468
CB
ALA
B
368
39.837
20.091
−25.542
1.00
15.07
C


ATOM
14472
C
ALA
B
368
39.140
18.424
−27.276
1.00
16.54
C


ATOM
14473
O
ALA
B
368
38.943
18.787
−28.433
1.00
16.90
O


ATOM
14475
N
PHE
B
369
39.750
17.280
−26.983
1.00
16.71
N


ATOM
14476
CA
PHE
B
369
40.063
16.318
−28.025
1.00
16.46
C


ATOM
14478
CB
PHE
B
369
40.770
15.083
−27.458
1.00
17.02
C


ATOM
14481
CG
PHE
B
369
42.182
15.320
−26.992
1.00
19.76
C


ATOM
14482
CD1
PHE
B
369
42.980
16.313
−27.547
1.00
22.26
C


ATOM
14484
CE1
PHE
B
369
44.279
16.496
−27.114
1.00
21.94
C


ATOM
14486
CZ
PHE
B
369
44.807
15.679
−26.145
1.00
22.55
C


ATOM
14488
CE2
PHE
B
369
44.039
14.675
−25.601
1.00
21.91
C


ATOM
14490
CD2
PHE
B
369
42.735
14.496
−26.026
1.00
21.14
C


ATOM
14492
C
PHE
B
369
38.799
15.864
−28.740
1.00
15.48
C


ATOM
14493
O
PHE
B
369
38.787
15.763
−29.953
1.00
15.19
O


ATOM
14495
N
LEU
B
370
37.739
15.582
−27.986
1.00
15.87
N


ATOM
14496
CA
LEU
B
370
36.506
15.070
−28.577
1.00
15.80
C


ATOM
14498
CB
LEU
B
370
35.478
14.717
−27.500
1.00
16.05
C


ATOM
14501
CG
LEU
B
370
34.194
14.042
−28.015
1.00
15.11
C


ATOM
14503
CD1
LEU
B
370
34.509
12.770
−28.799
1.00
11.25
C


ATOM
14507
CD2
LEU
B
370
33.272
13.740
−26.857
1.00
13.79
C


ATOM
14511
C
LEU
B
370
35.906
16.086
−29.524
1.00
16.00
C


ATOM
14512
O
LEU
B
370
35.404
15.729
−30.598
1.00
17.16
O


ATOM
14514
N
GLN
B
371
35.958
17.349
−29.113
1.00
15.48
N


ATOM
14515
CA
GLN
B
371
35.486
18.454
−29.932
1.00
15.64
C


ATOM
14517
CB
GLN
B
371
35.634
19.777
−29.179
1.00
15.46
C


ATOM
14520
CG
GLN
B
371
35.206
21.006
−29.971
1.00
16.71
C


ATOM
14523
CD
GLN
B
371
33.746
20.985
−30.320
1.00
13.91
C


ATOM
14524
OE1
GLN
B
371
33.375
20.698
−31.450
1.00
15.41
O


ATOM
14525
NE2
GLN
B
371
32.904
21.275
−29.343
1.00
17.06
N


ATOM
14528
C
GLN
B
371
36.244
18.505
−31.259
1.00
15.65
C


ATOM
14529
O
GLN
B
371
35.633
18.714
−32.305
1.00
16.37
O


ATOM
14531
N
GLU
B
372
37.561
18.305
−31.215
1.00
15.73
N


ATOM
14532
CA
GLU
B
372
38.372
18.260
−32.435
1.00
16.08
C


ATOM
14534
CB
GLU
B
372
39.860
18.167
−32.110
1.00
16.01
C


ATOM
14537
CG
GLU
B
372
40.462
19.474
−31.687
1.00
19.10
C


ATOM
14540
CD
GLU
B
372
41.909
19.363
−31.252
1.00
23.05
C


ATOM
14541
OE1
GLU
B
372
42.475
18.251
−31.273
1.00
32.06
O


ATOM
14542
OE2
GLU
B
372
42.485
20.401
−30.881
1.00
27.32
O


ATOM
14543
C
GLU
B
372
37.970
17.103
−33.343
1.00
16.13
C


ATOM
14544
O
GLU
B
372
37.835
17.281
−34.550
1.00
17.33
O


ATOM
14546
N
ALA
B
373
37.760
15.928
−32.761
1.00
15.96
N


ATOM
14547
CA
ALA
B
373
37.333
14.772
−33.535
1.00
16.64
C


ATOM
14549
CB
ALA
B
373
37.282
13.518
−32.659
1.00
15.39
C


ATOM
14553
C
ALA
B
373
35.979
15.025
−34.198
1.00
17.35
C


ATOM
14554
O
ALA
B
373
35.781
14.658
−35.358
1.00
17.83
O


ATOM
14556
N
LYS
B
374
35.052
15.649
−33.471
1.00
18.32
N


ATOM
14557
CA
LYS
B
374
33.701
15.903
−34.010
1.00
19.70
C


ATOM
14559
CB
LYS
B
374
32.754
16.428
−32.929
1.00
20.53
C


ATOM
14562
CG
LYS
B
374
32.259
15.355
−31.976
1.00
25.09
C


ATOM
14565
CD
LYS
B
374
31.376
15.934
−30.869
1.00
28.97
C


ATOM
14568
CE
LYS
B
374
30.781
14.829
−30.006
1.00
29.47
C


ATOM
14571
NZ
LYS
B
374
30.201
15.348
−28.737
1.00
30.82
N


ATOM
14575
C
LYS
B
374
33.734
16.884
−35.176
1.00
18.71
C


ATOM
14576
O
LYS
B
374
33.002
16.723
−36.148
1.00
17.84
O


ATOM
14578
N
TRP
B
375
34.581
17.901
−35.065
1.00
18.32
N


ATOM
14579
CA
TRP
B
375
34.749
18.864
−36.139
1.00
17.88
C


ATOM
14581
CB
TRP
B
375
35.672
20.019
−35.724
1.00
16.84
C


ATOM
14584
CG
TRP
B
375
35.029
21.093
−34.901
1.00
15.31
C


ATOM
14585
CD1
TRP
B
375
33.709
21.445
−34.887
1.00
17.15
C


ATOM
14587
NE1
TRP
B
375
33.509
22.496
−34.031
1.00
13.26
N


ATOM
14589
CE2
TRP
B
375
34.707
22.857
−33.480
1.00
10.19
C


ATOM
14590
CD2
TRP
B
375
35.690
21.997
−34.007
1.00
13.16
C


ATOM
14591
CE3
TRP
B
375
37.020
22.161
−33.600
1.00
12.02
C


ATOM
14593
CZ3
TRP
B
375
37.319
23.167
−32.681
1.00
14.69
C


ATOM
14595
CH2
TRP
B
375
36.315
23.997
−32.170
1.00
16.14
C


ATOM
14597
CZ2
TRP
B
375
35.003
23.857
−32.558
1.00
13.73
C


ATOM
14599
C
TRP
B
375
35.314
18.177
−37.373
1.00
18.55
C


ATOM
14600
O
TRP
B
375
34.883
18.469
−38.487
1.00
19.56
O


ATOM
14602
N
LEU
B
376
36.284
17.285
−37.179
1.00
19.65
N


ATOM
14603
CA
LEU
B
376
36.853
16.530
−38.292
1.00
21.62
C


ATOM
14605
CB
LEU
B
376
38.060
15.700
−37.857
1.00
23.00
C


ATOM
14608
CG
LEU
B
376
38.856
15.074
−39.015
1.00
29.39
C


ATOM
14610
CD1
LEU
B
376
39.681
16.142
−39.751
1.00
34.17
C


ATOM
14614
CD2
LEU
B
376
39.758
13.934
−38.529
1.00
30.95
C


ATOM
14618
C
LEU
B
376
35.791
15.629
−38.920
1.00
20.99
C


ATOM
14619
O
LEU
B
376
35.558
15.694
−40.126
1.00
21.94
O


ATOM
14621
N
TYR
B
377
35.132
14.817
−38.103
1.00
19.88
N


ATOM
14622
CA
TYR
B
377
34.070
13.935
−38.594
1.00
20.68
C


ATOM
14624
CB
TYR
B
377
33.384
13.228
−37.430
1.00
21.02
C


ATOM
14627
CG
TYR
B
377
32.350
12.194
−37.827
1.00
22.29
C


ATOM
14628
CD1
TYR
B
377
32.714
10.866
−38.056
1.00
25.15
C


ATOM
14630
CE1
TYR
B
377
31.759
9.908
−38.407
1.00
24.92
C


ATOM
14632
CZ
TYR
B
377
30.428
10.278
−38.521
1.00
24.98
C


ATOM
14633
OH
TYR
B
377
29.489
9.340
−38.866
1.00
28.20
O


ATOM
14635
CE2
TYR
B
377
30.042
11.585
−38.295
1.00
22.14
C


ATOM
14637
CD2
TYR
B
377
31.005
12.534
−37.945
1.00
23.27
C


ATOM
14639
C
TYR
B
377
33.020
14.689
−39.389
1.00
21.44
C


ATOM
14640
O
TYR
B
377
32.677
14.290
−40.496
1.00
22.45
O


ATOM
14642
N
ASN
B
378
32.508
15.774
−38.814
1.00
22.31
N


ATOM
14643
CA
ASN
B
378
31.422
16.545
−39.427
1.00
22.28
C


ATOM
14645
CB
ASN
B
378
30.632
17.294
−38.349
1.00
22.49
C


ATOM
14648
CG
ASN
B
378
29.792
16.369
−37.490
1.00
22.55
C


ATOM
14649
OD1
ASN
B
378
29.269
15.360
−37.962
1.00
27.91
O


ATOM
14650
ND2
ASN
B
378
29.634
16.728
−36.227
1.00
21.74
N


ATOM
14653
C
ASN
B
378
31.892
17.543
−40.483
1.00
22.22
C


ATOM
14654
O
ASN
B
378
31.081
18.265
−41.054
1.00
20.28
O


ATOM
14656
N
LYS
B
379
33.199
17.585
−40.730
1.00
23.59
N


ATOM
14657
CA
LYS
B
379
33.783
18.496
−41.711
1.00
25.11
C


ATOM
14659
CB
LYS
B
379
33.382
18.077
−43.135
1.00
25.86
C


ATOM
14662
CG
LYS
B
379
33.990
16.735
−43.560
1.00
29.75
C


ATOM
14665
CD
LYS
B
379
33.339
16.169
−44.820
1.00
37.47
C


ATOM
14668
CE
LYS
B
379
34.249
15.148
−45.512
1.00
41.99
C


ATOM
14671
NZ
LYS
B
379
34.924
14.228
−44.545
1.00
43.15
N


ATOM
14675
C
LYS
B
379
33.416
19.954
−41.409
1.00
24.70
C


ATOM
14676
O
LYS
B
379
33.180
20.749
−42.316
1.00
24.74
O


ATOM
14678
N
SER
B
380
33.391
20.291
−40.117
1.00
23.98
N


ATOM
14679
CA
SER
B
380
33.058
21.637
−39.666
1.00
22.30
C


ATOM
14681
CB
SER
B
380
32.828
21.671
−38.154
1.00
22.07
C


ATOM
14684
OG
SER
B
380
31.557
21.141
−37.813
1.00
24.80
O


ATOM
14686
C
SER
B
380
34.160
22.615
−40.035
1.00
21.05
C


ATOM
14687
O
SER
B
380
35.304
22.230
−40.254
1.00
18.77
O


ATOM
14689
N
THR
B
381
33.797
23.890
−40.096
1.00
20.77
N


ATOM
14690
CA
THR
B
381
34.729
24.940
−40.480
1.00
20.78
C


ATOM
14692
CB
THR
B
381
34.436
25.427
−41.921
1.00
21.72
C


ATOM
14694
OG1
THR
B
381
33.019
25.577
−42.102
1.00
22.85
O


ATOM
14696
CG2
THR
B
381
34.954
24.420
−42.932
1.00
20.93
C


ATOM
14700
C
THR
B
381
34.639
26.079
−39.470
1.00
19.47
C


ATOM
14701
O
THR
B
381
34.331
27.215
−39.828
1.00
19.25
O


ATOM
14703
N
PRO
B
382
34.930
25.778
−38.192
1.00
18.98
N


ATOM
14704
CA
PRO
B
382
34.801
26.794
−37.149
1.00
18.70
C


ATOM
14706
CB
PRO
B
382
35.165
26.033
−35.875
1.00
19.04
C


ATOM
14709
CG
PRO
B
382
36.047
24.915
−36.338
1.00
18.25
C


ATOM
14712
CD
PRO
B
382
35.543
24.534
−37.679
1.00
18.70
C


ATOM
14715
C
PRO
B
382
35.739
27.987
−37.348
1.00
18.01
C


ATOM
14716
O
PRO
B
382
36.781
27.865
−37.989
1.00
18.37
O


ATOM
14717
N
THR
B
383
35.357
29.131
−36.797
1.00
17.46
N


ATOM
14718
CA
THR
B
383
36.167
30.338
−36.876
1.00
17.12
C


ATOM
14720
CB
THR
B
383
35.445
31.535
−36.243
1.00
18.32
C


ATOM
14722
OG1
THR
B
383
35.274
31.293
−34.839
1.00
17.62
O


ATOM
14724
CG2
THR
B
383
34.075
31.785
−36.908
1.00
14.15
C


ATOM
14728
C
THR
B
383
37.460
30.139
−36.104
1.00
17.10
C


ATOM
14729
O
THR
B
383
37.595
29.183
−35.335
1.00
18.78
O


ATOM
14731
N
PHE
B
384
38.404
31.051
−36.277
1.00
15.89
N


ATOM
14732
CA
PHE
B
384
39.644
30.986
−35.520
1.00
15.57
C


ATOM
14734
CB
PHE
B
384
40.625
32.062
−35.984
1.00
15.29
C


ATOM
14737
CG
PHE
B
384
41.838
32.174
−35.120
1.00
15.96
C


ATOM
14738
CD1
PHE
B
384
42.932
31.347
−35.334
1.00
13.73
C


ATOM
14740
CE1
PHE
B
384
44.054
31.442
−34.527
1.00
14.08
C


ATOM
14742
CZ
PHE
B
384
44.088
32.370
−33.480
1.00
14.22
C


ATOM
14744
CE2
PHE
B
384
42.997
33.194
−33.250
1.00
13.73
C


ATOM
14746
CD2
PHE
B
384
41.878
33.093
−34.070
1.00
17.46
C


ATOM
14748
C
PHE
B
384
39.406
31.090
−34.007
1.00
15.87
C


ATOM
14749
O
PHE
B
384
40.029
30.369
−33.238
1.00
16.32
O


ATOM
14751
N
ASP
B
385
38.503
31.969
−33.577
1.00
17.06
N


ATOM
14752
CA
ASP
B
385
38.250
32.138
−32.141
1.00
17.47
C


ATOM
14754
CB
ASP
B
385
37.252
33.267
−31.860
1.00
16.73
C


ATOM
14757
CG
ASP
B
385
37.820
34.650
−32.148
1.00
13.98
C


ATOM
14758
OD1
ASP
B
385
39.040
34.807
−32.288
1.00
13.45
O


ATOM
14759
OD2
ASP
B
385
37.025
35.597
−32.240
1.00
20.82
O


ATOM
14760
C
ASP
B
385
37.761
30.847
−31.494
1.00
18.08
C


ATOM
14761
O
ASP
B
385
38.224
30.494
−30.416
1.00
18.18
O


ATOM
14763
N
ASP
B
386
36.839
30.149
−32.158
1.00
18.96
N


ATOM
14764
CA
ASP
B
386
36.289
28.894
−31.641
1.00
19.56
C


ATOM
14766
CB
ASP
B
386
35.018
28.487
−32.386
1.00
19.25
C


ATOM
14769
CG
ASP
B
386
33.848
29.394
−32.086
1.00
25.69
C


ATOM
14770
OD1
ASP
B
386
33.968
30.349
−31.275
1.00
29.37
O


ATOM
14771
OD2
ASP
B
386
32.782
29.142
−32.680
1.00
37.04
O


ATOM
14772
C
ASP
B
386
37.289
27.759
−31.726
1.00
18.80
C


ATOM
14773
O
ASP
B
386
37.462
27.016
−30.762
1.00
18.56
O


ATOM
14775
N
TYR
B
387
37.939
27.617
−32.875
1.00
18.41
N


ATOM
14776
CA
TYR
B
387
38.939
26.563
−33.035
1.00
18.76
C


ATOM
14778
CB
TYR
B
387
39.518
26.546
−34.449
1.00
19.09
C


ATOM
14781
CG
TYR
B
387
40.560
25.476
−34.630
1.00
21.22
C


ATOM
14782
CD1
TYR
B
387
40.191
24.152
−34.861
1.00
25.12
C


ATOM
14784
CE1
TYR
B
387
41.139
23.157
−35.012
1.00
24.90
C


ATOM
14786
CZ
TYR
B
387
42.476
23.478
−34.932
1.00
29.40
C


ATOM
14787
OH
TYR
B
387
43.432
22.495
−35.087
1.00
32.17
O


ATOM
14789
CE2
TYR
B
387
42.865
24.789
−34.698
1.00
28.78
C


ATOM
14791
CD2
TYR
B
387
41.907
25.776
−34.547
1.00
23.54
C


ATOM
14793
C
TYR
B
387
40.063
26.752
−32.030
1.00
18.02
C


ATOM
14794
O
TYR
B
387
40.387
25.852
−31.267
1.00
17.96
O


ATOM
14796
N
PHE
B
388
40.658
27.933
−32.033
1.00
18.09
N


ATOM
14797
CA
PHE
B
388
41.744
28.224
−31.109
1.00
17.94
C


ATOM
14799
CB
PHE
B
388
42.326
29.600
−31.405
1.00
18.49
C


ATOM
14802
CG
PHE
B
388
43.524
29.923
−30.582
1.00
20.80
C


ATOM
14803
CD1
PHE
B
388
44.731
29.301
−30.834
1.00
15.90
C


ATOM
14805
CE1
PHE
B
388
45.838
29.587
−30.068
1.00
18.77
C


ATOM
14807
CZ
PHE
B
388
45.752
30.499
−29.037
1.00
21.03
C


ATOM
14809
CE2
PHE
B
388
44.556
31.122
−28.770
1.00
23.90
C


ATOM
14811
CD2
PHE
B
388
43.443
30.831
−29.538
1.00
22.37
C


ATOM
14813
C
PHE
B
388
41.312
28.153
−29.642
1.00
16.94
C


ATOM
14814
O
PHE
B
388
42.097
27.758
−28.790
1.00
17.81
O


ATOM
14816
N
GLY
B
389
40.068
28.534
−29.353
1.00
16.08
N


ATOM
14817
CA
GLY
B
389
39.525
28.474
−28.000
1.00
14.92
C


ATOM
14820
C
GLY
B
389
39.608
27.070
−27.447
1.00
15.74
C


ATOM
14821
O
GLY
B
389
39.961
26.868
−26.287
1.00
17.64
O


ATOM
14823
N
ASN
B
390
39.282
26.101
−28.297
1.00
15.28
N


ATOM
14824
CA
ASN
B
390
39.427
24.699
−27.978
1.00
14.62
C


ATOM
14826
CB
ASN
B
390
38.534
23.872
−28.897
1.00
14.84
C


ATOM
14829
CG
ASN
B
390
38.448
22.421
−28.472
1.00
14.88
C


ATOM
14830
OD1
ASN
B
390
37.918
22.112
−27.413
1.00
19.79
O


ATOM
14831
ND2
ASN
B
390
38.967
21.526
−29.296
1.00
8.97
N


ATOM
14834
C
ASN
B
390
40.877
24.224
−28.105
1.00
15.00
C


ATOM
14835
O
ASN
B
390
41.323
23.397
−27.322
1.00
16.30
O


ATOM
14837
N
ALA
B
391
41.603
24.741
−29.092
1.00
15.37
N


ATOM
14838
CA
ALA
B
391
42.967
24.287
−29.385
1.00
14.99
C


ATOM
14840
CB
ALA
B
391
43.468
24.877
−30.690
1.00
14.64
C


ATOM
14844
C
ALA
B
391
43.953
24.580
−28.278
1.00
15.29
C


ATOM
14845
O
ALA
B
391
44.803
23.758
−27.996
1.00
17.57
O


ATOM
14847
N
TRP
B
392
43.871
25.732
−27.634
1.00
16.03
N


ATOM
14848
CA
TRP
B
392
44.813
25.982
−26.546
1.00
17.23
C


ATOM
14850
CB
TRP
B
392
44.912
27.476
−26.167
1.00
18.07
C


ATOM
14853
CG
TRP
B
392
43.728
28.128
−25.525
1.00
17.09
C


ATOM
14854
CD1
TRP
B
392
42.868
29.015
−26.111
1.00
20.34
C


ATOM
14856
NE1
TRP
B
392
41.923
29.427
−25.208
1.00
18.34
N


ATOM
14858
CE2
TRP
B
392
42.171
28.826
−24.003
1.00
14.64
C


ATOM
14859
CD2
TRP
B
392
43.311
28.006
−24.163
1.00
15.29
C


ATOM
14860
CE3
TRP
B
392
43.778
27.270
−23.063
1.00
16.69
C


ATOM
14862
CZ3
TRP
B
392
43.107
27.385
−21.857
1.00
19.38
C


ATOM
14864
CH2
TRP
B
392
41.972
28.220
−21.732
1.00
18.81
C


ATOM
14866
CZ2
TRP
B
392
41.494
28.939
−22.792
1.00
15.59
C


ATOM
14868
C
TRP
B
392
44.603
25.073
−25.330
1.00
17.36
C


ATOM
14869
O
TRP
B
392
45.524
24.889
−24.545
1.00
19.29
O


ATOM
14871
N
LYS
B
393
43.421
24.480
−25.195
1.00
17.67
N


ATOM
14872
CA
LYS
B
393
43.154
23.466
−24.162
1.00
18.16
C


ATOM
14874
CB
LYS
B
393
41.652
23.391
−23.843
1.00
19.24
C


ATOM
14877
CG
LYS
B
393
41.018
24.718
−23.352
1.00
24.75
C


ATOM
14880
CD
LYS
B
393
39.556
24.527
−22.950
1.00
31.62
C


ATOM
14883
CE
LYS
B
393
38.894
25.820
−22.454
1.00
35.24
C


ATOM
14886
NZ
LYS
B
393
38.513
26.723
−23.584
1.00
41.45
N


ATOM
14890
C
LYS
B
393
43.642
22.081
−24.598
1.00
17.55
C


ATOM
14891
O
LYS
B
393
44.168
21.318
−23.796
1.00
16.86
O


ATOM
14893
N
SER
B
394
43.460
21.754
−25.872
1.00
18.02
N


ATOM
14894
CA
SER
B
394
43.863
20.444
−26.389
1.00
18.30
C


ATOM
14896
CB
SER
B
394
43.247
20.183
−27.759
1.00
18.56
C


ATOM
14899
OG
SER
B
394
43.723
21.113
−28.708
1.00
19.78
O


ATOM
14901
C
SER
B
394
45.369
20.307
−26.485
1.00
18.26
C


ATOM
14902
O
SER
B
394
45.872
19.196
−26.626
1.00
18.34
O


ATOM
14904
N
SER
B
395
46.085
21.432
−26.420
1.00
18.15
N


ATOM
14905
CA
SER
B
395
47.545
21.415
−26.340
1.00
17.82
C


ATOM
14907
CB
SER
B
395
48.105
22.841
−26.221
1.00
18.00
C


ATOM
14910
OG
SER
B
395
47.883
23.400
−24.931
1.00
15.24
O


ATOM
14912
C
SER
B
395
47.996
20.594
−25.132
1.00
18.16
C


ATOM
14913
O
SER
B
395
49.087
20.028
−25.142
1.00
18.62
O


ATOM
14915
N
SER
B
396
47.135
20.548
−24.110
1.00
17.42
N


ATOM
14916
CA
SER
B
396
47.403
19.930
−22.808
1.00
17.40
C


ATOM
14918
CB
SER
B
396
48.154
18.588
−22.919
1.00
17.74
C


ATOM
14921
OG
SER
B
396
49.556
18.761
−23.041
1.00
16.91
O


ATOM
14923
C
SER
B
396
48.138
20.885
−21.878
1.00
16.63
C


ATOM
14924
O
SER
B
396
48.479
20.515
−20.765
1.00
16.92
O


ATOM
14926
N
GLY
B
397
48.356
22.120
−22.320
1.00
16.76
N


ATOM
14927
CA
GLY
B
397
49.013
23.133
−21.487
1.00
16.96
C


ATOM
14930
C
GLY
B
397
48.332
23.369
−20.146
1.00
16.88
C


ATOM
14931
O
GLY
B
397
48.968
23.309
−19.100
1.00
17.22
O


ATOM
14933
N
PRO
B
398
47.027
23.662
−20.162
1.00
16.83
N


ATOM
14934
CA
PRO
B
398
46.339
23.817
−18.884
1.00
16.78
C


ATOM
14936
CB
PRO
B
398
44.885
24.081
−19.294
1.00
16.50
C


ATOM
14939
CG
PRO
B
398
44.985
24.636
−20.671
1.00
17.53
C


ATOM
14942
CD
PRO
B
398
46.141
23.943
−21.304
1.00
16.58
C


ATOM
14945
C
PRO
B
398
46.447
22.590
−17.972
1.00
16.11
C


ATOM
14946
O
PRO
B
398
46.615
22.746
−16.774
1.00
16.39
O


ATOM
14947
N
LEU
B
399
46.360
21.387
−18.529
1.00
17.24
N


ATOM
14948
CA
LEU
B
399
46.457
20.162
−17.722
1.00
17.58
C


ATOM
14950
CB
LEU
B
399
46.142
18.910
−18.550
1.00
17.05
C


ATOM
14953
CG
LEU
B
399
46.180
17.581
−17.784
1.00
20.38
C


ATOM
14955
CD1
LEU
B
399
45.007
16.668
−18.156
1.00
21.31
C


ATOM
14959
CD2
LEU
B
399
47.525
16.843
−17.966
1.00
19.29
C


ATOM
14963
C
LEU
B
399
47.841
20.035
−17.090
1.00
18.34
C


ATOM
14964
O
LEU
B
399
47.950
19.741
−15.897
1.00
18.31
O


ATOM
14966
N
GLN
B
400
48.888
20.255
−17.886
1.00
17.51
N


ATOM
14967
CA
GLN
B
400
50.257
20.166
−17.387
1.00
17.30
C


ATOM
14969
CB
GLN
B
400
51.264
20.494
−18.485
1.00
17.79
C


ATOM
14972
CG
GLN
B
400
51.391
19.417
−19.548
1.00
20.06
C


ATOM
14975
CD
GLN
B
400
52.402
19.758
−20.624
1.00
21.76
C


ATOM
14976
OE1
GLN
B
400
53.447
20.341
−20.348
1.00
25.47
O


ATOM
14977
NE2
GLN
B
400
52.106
19.373
−21.858
1.00
25.16
N


ATOM
14980
C
GLN
B
400
50.464
21.104
−16.212
1.00
17.25
C


ATOM
14981
O
GLN
B
400
51.066
20.731
−15.205
1.00
18.72
O


ATOM
14983
N
LEU
B
401
49.939
22.311
−16.325
1.00
16.70
N


ATOM
14984
CA
LEU
B
401
50.162
23.318
−15.300
1.00
17.29
C


ATOM
14986
CB
LEU
B
401
49.900
24.714
−15.861
1.00
16.74
C


ATOM
14989
CG
LEU
B
401
50.960
25.122
−16.894
1.00
17.79
C


ATOM
14991
CD1
LEU
B
401
50.728
26.535
−17.421
1.00
16.38
C


ATOM
14995
CD2
LEU
B
401
52.378
24.984
−16.308
1.00
12.70
C


ATOM
14999
C
LEU
B
401
49.350
23.069
−14.034
1.00
18.12
C


ATOM
15000
O
LEU
B
401
49.826
23.339
−12.926
1.00
18.82
O


ATOM
15002
N
VAL
B
402
48.135
22.545
−14.187
1.00
17.92
N


ATOM
15003
CA
VAL
B
402
47.360
22.108
−13.031
1.00
17.06
C


ATOM
15005
CB
VAL
B
402
46.025
21.493
−13.444
1.00
16.81
C


ATOM
15007
CG1
VAL
B
402
45.356
20.820
−12.249
1.00
16.61
C


ATOM
15011
CG2
VAL
B
402
45.128
22.560
−14.039
1.00
13.84
C


ATOM
15015
C
VAL
B
402
48.169
21.077
−12.246
1.00
17.74
C


ATOM
15016
O
VAL
B
402
48.323
21.194
−11.040
1.00
18.28
O


ATOM
15018
N
PHE
B
403
48.707
20.082
−12.946
1.00
17.77
N


ATOM
15019
CA
PHE
B
403
49.545
19.079
−12.316
1.00
17.93
C


ATOM
15021
CB
PHE
B
403
49.859
17.941
−13.287
1.00
18.14
C


ATOM
15024
CG
PHE
B
403
48.789
16.884
−13.344
1.00
17.67
C


ATOM
15025
CD1
PHE
B
403
47.728
16.995
−14.229
1.00
18.37
C


ATOM
15027
CE1
PHE
B
403
46.745
16.023
−14.279
1.00
19.30
C


ATOM
15029
CZ
PHE
B
403
46.817
14.920
−13.436
1.00
20.55
C


ATOM
15031
CE2
PHE
B
403
47.878
14.795
−12.556
1.00
19.05
C


ATOM
15033
CD2
PHE
B
403
48.851
15.772
−12.511
1.00
17.45
C


ATOM
15035
C
PHE
B
403
50.828
19.680
−11.755
1.00
18.51
C


ATOM
15036
O
PHE
B
403
51.283
19.268
−10.684
1.00
18.72
O


ATOM
15038
N
ALA
B
404
51.405
20.649
−12.465
1.00
18.05
N


ATOM
15039
CA
ALA
B
404
52.610
21.328
−11.975
1.00
17.65
C


ATOM
15041
CB
ALA
B
404
53.228
22.195
−13.060
1.00
16.65
C


ATOM
15045
C
ALA
B
404
52.313
22.159
−10.723
1.00
17.63
C


ATOM
15046
O
ALA
B
404
53.128
22.211
−9.804
1.00
17.86
O


ATOM
15048
N
TYR
B
405
51.146
22.797
−10.676
1.00
17.74
N


ATOM
15049
CA
TYR
B
405
50.737
23.536
−9.478
1.00
17.66
C


ATOM
15051
CB
TYR
B
405
49.301
24.061
−9.606
1.00
16.56
C


ATOM
15054
CG
TYR
B
405
48.763
24.664
−8.334
1.00
18.44
C


ATOM
15055
CD1
TYR
B
405
49.020
25.994
−8.005
1.00
21.48
C


ATOM
15057
CE1
TYR
B
405
48.528
26.552
−6.829
1.00
22.32
C


ATOM
15059
CZ
TYR
B
405
47.777
25.776
−5.966
1.00
24.60
C


ATOM
15060
OH
TYR
B
405
47.293
26.318
−4.794
1.00
24.67
O


ATOM
15062
CE2
TYR
B
405
47.505
24.454
−6.277
1.00
20.72
C


ATOM
15064
CD2
TYR
B
405
47.997
23.906
−7.450
1.00
19.91
C


ATOM
15066
C
TYR
B
405
50.870
22.651
−8.234
1.00
17.91
C


ATOM
15067
O
TYR
B
405
51.516
23.035
−7.265
1.00
18.91
O


ATOM
15069
N
PHE
B
406
50.285
21.459
−8.271
1.00
17.90
N


ATOM
15070
CA
PHE
B
406
50.287
20.585
−7.092
1.00
18.27
C


ATOM
15072
CB
PHE
B
406
49.269
19.452
−7.250
1.00
17.96
C


ATOM
15075
CG
PHE
B
406
47.847
19.930
−7.236
1.00
17.04
C


ATOM
15076
CD1
PHE
B
406
47.254
20.334
−6.050
1.00
18.42
C


ATOM
15078
CE1
PHE
B
406
45.944
20.789
−6.029
1.00
17.34
C


ATOM
15080
CZ
PHE
B
406
45.207
20.846
−7.210
1.00
16.34
C


ATOM
15082
CE2
PHE
B
406
45.787
20.457
−8.394
1.00
14.13
C


ATOM
15084
CD2
PHE
B
406
47.110
20.009
−8.406
1.00
17.40
C


ATOM
15086
C
PHE
B
406
51.673
20.045
−6.735
1.00
18.49
C


ATOM
15087
O
PHE
B
406
51.958
19.827
−5.568
1.00
19.16
O


ATOM
15089
N
ALA
B
407
52.537
19.868
−7.729
1.00
19.29
N


ATOM
15090
CA
ALA
B
407
53.919
19.457
−7.488
1.00
20.20
C


ATOM
15092
CB
ALA
B
407
54.481
18.799
−8.728
1.00
20.07
C


ATOM
15096
C
ALA
B
407
54.844
20.601
−7.042
1.00
22.13
C


ATOM
15097
O
ALA
B
407
55.879
20.348
−6.435
1.00
22.29
O


ATOM
15099
N
VAL
B
408
54.487
21.847
−7.353
1.00
24.41
N


ATOM
15100
CA
VAL
B
408
55.353
22.999
−7.072
1.00
25.78
C


ATOM
15102
CB
VAL
B
408
55.368
23.991
−8.254
1.00
25.81
C


ATOM
15104
CG1
VAL
B
408
56.010
25.309
−7.853
1.00
25.93
C


ATOM
15108
CG2
VAL
B
408
56.095
23.385
−9.447
1.00
25.72
C


ATOM
15112
C
VAL
B
408
54.933
23.748
−5.809
1.00
28.22
C


ATOM
15113
O
VAL
B
408
55.776
24.151
−5.017
1.00
28.39
O


ATOM
15115
N
VAL
B
409
53.632
23.936
−5.626
1.00
31.39
N


ATOM
15116
CA
VAL
B
409
53.120
24.679
−4.484
1.00
33.83
C


ATOM
15118
CB
VAL
B
409
51.655
25.106
−4.702
1.00
33.75
C


ATOM
15120
CG1
VAL
B
409
51.042
25.639
−3.421
1.00
35.27
C


ATOM
15124
CG2
VAL
B
409
51.582
26.156
−5.785
1.00
34.78
C


ATOM
15128
C
VAL
B
409
53.239
23.851
−3.210
1.00
36.82
C


ATOM
15129
O
VAL
B
409
52.872
22.680
−3.182
1.00
37.37
O


ATOM
15131
N
GLN
B
410
53.762
24.481
−2.161
1.00
39.92
N


ATOM
15132
CA
GLN
B
410
53.922
23.848
−0.857
1.00
41.63
C


ATOM
15134
CB
GLN
B
410
54.907
24.669
−0.016
1.00
43.82
C


ATOM
15137
CG
GLN
B
410
55.557
23.922
1.151
1.00
49.70
C


ATOM
15140
CD
GLN
B
410
56.469
24.829
1.971
1.00
56.21
C


ATOM
15141
OE1
GLN
B
410
57.165
25.686
1.417
1.00
60.99
O


ATOM
15142
NE2
GLN
B
410
56.465
24.651
3.293
1.00
55.19
N


ATOM
15145
C
GLN
B
410
52.559
23.749
−0.168
1.00
40.91
C


ATOM
15146
O
GLN
B
410
52.087
22.648
0.131
1.00
41.39
O


ATOM
15148
N
ASN
B
411
51.919
24.898
0.055
1.00
39.55
N


ATOM
15149
CA
ASN
B
411
50.586
24.941
0.671
1.00
38.41
C


ATOM
15151
CB
ASN
B
411
50.572
25.915
1.847
1.00
38.05
C


ATOM
15154
CG
ASN
B
411
51.384
25.412
3.018
1.00
37.70
C


ATOM
15155
OD1
ASN
B
411
51.090
24.359
3.583
1.00
34.08
O


ATOM
15156
ND2
ASN
B
411
52.421
26.160
3.384
1.00
38.90
N


ATOM
15159
C
ASN
B
411
49.486
25.315
−0.317
1.00
37.54
C


ATOM
15160
O
ASN
B
411
49.443
26.437
−0.824
1.00
37.31
O


ATOM
15162
N
ILE
B
412
48.587
24.369
−0.561
1.00
37.00
N


ATOM
15163
CA
ILE
B
412
47.490
24.550
−1.501
1.00
37.00
C


ATOM
15165
CB
ILE
B
412
46.743
23.229
−1.708
1.00
37.15
C


ATOM
15167
CG1
ILE
B
412
47.648
22.208
−2.390
1.00
39.56
C


ATOM
15170
CD1
ILE
B
412
47.120
20.791
−2.291
1.00
40.67
C


ATOM
15174
CG2
ILE
B
412
45.496
23.439
−2.539
1.00
41.23
C


ATOM
15178
C
ILE
B
412
46.506
25.588
−0.971
1.00
36.72
C


ATOM
15179
O
ILE
B
412
46.360
25.743
0.242
1.00
36.62
O


ATOM
15181
N
LYS
B
413
45.844
26.299
−1.883
1.00
36.30
N


ATOM
15182
CA
LYS
B
413
44.857
27.309
−1.519
1.00
36.38
C


ATOM
15184
CB
LYS
B
413
45.453
28.708
−1.700
1.00
36.79
C


ATOM
15187
CG
LYS
B
413
46.673
28.932
−0.818
1.00
40.58
C


ATOM
15190
CD
LYS
B
413
47.210
30.339
−0.877
1.00
46.29
C


ATOM
15193
CE
LYS
B
413
48.338
30.512
0.139
1.00
47.89
C


ATOM
15196
NZ
LYS
B
413
48.983
31.846
0.044
1.00
49.06
N


ATOM
15200
C
LYS
B
413
43.622
27.134
−2.375
1.00
36.15
C


ATOM
15201
O
LYS
B
413
43.727
27.011
−3.591
1.00
37.20
O


ATOM
15203
N
LYS
B
414
42.451
27.117
−1.748
1.00
35.96
N


ATOM
15204
CA
LYS
B
414
41.207
26.837
−2.467
1.00
35.71
C


ATOM
15206
CB
LYS
B
414
40.017
26.794
−1.503
1.00
36.25
C


ATOM
15209
CG
LYS
B
414
38.715
26.292
−2.139
1.00
40.45
C


ATOM
15212
CD
LYS
B
414
37.639
25.973
−1.090
1.00
45.77
C


ATOM
15215
CE
LYS
B
414
37.054
27.234
−0.451
1.00
46.82
C


ATOM
15218
NZ
LYS
B
414
36.316
28.078
−1.437
1.00
47.29
N


ATOM
15222
C
LYS
B
414
40.940
27.854
−3.576
1.00
34.67
C


ATOM
15223
O
LYS
B
414
40.404
27.506
−4.627
1.00
34.64
O


ATOM
15225
N
GLU
B
415
41.304
29.110
−3.344
1.00
33.56
N


ATOM
15226
CA
GLU
B
415
41.075
30.142
−4.346
1.00
33.01
C


ATOM
15228
CB
GLU
B
415
41.354
31.536
−3.776
1.00
33.31
C


ATOM
15231
CG
GLU
B
415
40.982
32.664
−4.740
1.00
38.15
C


ATOM
15234
CD
GLU
B
415
41.040
34.045
−4.102
1.00
44.38
C


ATOM
15235
OE1
GLU
B
415
42.046
34.354
−3.421
1.00
48.38
O


ATOM
15236
OE2
GLU
B
415
40.082
34.826
−4.297
1.00
46.55
O


ATOM
15237
C
GLU
B
415
41.939
29.883
−5.581
1.00
30.64
C


ATOM
15238
O
GLU
B
415
41.467
29.987
−6.715
1.00
29.03
O


ATOM
15240
N
GLU
B
416
43.198
29.533
−5.347
1.00
28.88
N


ATOM
15241
CA
GLU
B
416
44.134
29.287
−6.433
1.00
28.18
C


ATOM
15243
CB
GLU
B
416
45.512
28.927
−5.879
1.00
27.80
C


ATOM
15246
CG
GLU
B
416
46.251
30.110
−5.281
1.00
28.18
C


ATOM
15249
CD
GLU
B
416
47.632
29.748
−4.785
1.00
30.77
C


ATOM
15250
OE1
GLU
B
416
47.831
28.618
−4.304
1.00
38.12
O


ATOM
15251
OE2
GLU
B
416
48.532
30.597
−4.867
1.00
39.33
O


ATOM
15252
C
GLU
B
416
43.635
28.194
−7.372
1.00
28.47
C


ATOM
15253
O
GLU
B
416
43.665
28.366
−8.590
1.00
27.41
O


ATOM
15255
N
ILE
B
417
43.169
27.081
−6.803
1.00
28.87
N


ATOM
15256
CA
ILE
B
417
42.705
25.946
−7.609
1.00
29.70
C


ATOM
15258
CB
ILE
B
417
42.712
24.589
−6.825
1.00
30.57
C


ATOM
15260
CG1
ILE
B
417
41.792
24.611
−5.612
1.00
34.16
C


ATOM
15263
CD1
ILE
B
417
42.218
23.603
−4.535
1.00
37.78
C


ATOM
15267
CG2
ILE
B
417
44.122
24.239
−6.351
1.00
30.55
C


ATOM
15271
C
ILE
B
417
41.349
26.216
−8.258
1.00
28.90
C


ATOM
15272
O
ILE
B
417
41.078
25.728
−9.350
1.00
29.33
O


ATOM
15274
N
GLU
B
418
40.506
27.008
−7.609
1.00
29.32
N


ATOM
15275
CA
GLU
B
418
39.269
27.470
−8.244
1.00
29.58
C


ATOM
15277
CB
GLU
B
418
38.429
28.291
−7.270
1.00
29.92
C


ATOM
15280
CG
GLU
B
418
37.592
27.423
−6.339
1.00
35.12
C


ATOM
15283
CD
GLU
B
418
36.912
28.207
−5.228
1.00
40.09
C


ATOM
15284
OE1
GLU
B
418
37.154
29.433
−5.101
1.00
39.64
O


ATOM
15285
OE2
GLU
B
418
36.134
27.581
−4.474
1.00
42.62
O


ATOM
15286
C
GLU
B
418
39.570
28.281
−9.506
1.00
29.38
C


ATOM
15287
O
GLU
B
418
38.905
28.118
−10.532
1.00
29.16
O


ATOM
15289
N
ASN
B
419
40.584
29.139
−9.430
1.00
28.62
N


ATOM
15290
CA
ASN
B
419
40.996
29.925
−10.578
1.00
27.70
C


ATOM
15292
CB
ASN
B
419
41.862
31.103
−10.139
1.00
27.76
C


ATOM
15295
CG
ASN
B
419
41.027
32.329
−9.789
1.00
30.05
C


ATOM
15296
OD1
ASN
B
419
40.426
32.946
−10.670
1.00
33.99
O


ATOM
15297
ND2
ASN
B
419
40.974
32.678
−8.505
1.00
28.62
N


ATOM
15300
C
ASN
B
419
41.692
29.085
−11.638
1.00
28.30
C


ATOM
15301
O
ASN
B
419
41.631
29.414
−12.826
1.00
28.75
O


ATOM
15303
N
LEU
B
420
42.343
28.000
−11.221
1.00
28.29
N


ATOM
15304
CA
LEU
B
420
42.918
27.050
−12.173
1.00
27.94
C


ATOM
15306
CB
LEU
B
420
43.743
25.964
−11.468
1.00
28.03
C


ATOM
15309
CG
LEU
B
420
45.145
26.316
−10.953
1.00
27.38
C


ATOM
15311
CD1
LEU
B
420
45.698
25.181
−10.100
1.00
23.69
C


ATOM
15315
CD2
LEU
B
420
46.089
26.618
−12.097
1.00
23.35
C


ATOM
15319
C
LEU
B
420
41.800
26.406
−12.987
1.00
29.01
C


ATOM
15320
O
LEU
B
420
41.906
26.290
−14.202
1.00
28.72
O


ATOM
15322
N
GLN
B
421
40.724
26.006
−12.314
1.00
30.82
N


ATOM
15323
CA
GLN
B
421
39.575
25.395
−12.990
1.00
32.50
C


ATOM
15325
CB
GLN
B
421
38.576
24.834
−11.970
1.00
32.85
C


ATOM
15328
CG
GLN
B
421
39.116
23.657
−11.189
1.00
36.59
C


ATOM
15331
CD
GLN
B
421
38.050
22.914
−10.420
1.00
40.68
C


ATOM
15332
OE1
GLN
B
421
37.934
21.689
−10.524
1.00
44.45
O


ATOM
15333
NE2
GLN
B
421
37.265
23.646
−9.636
1.00
42.01
N


ATOM
15336
C
GLN
B
421
38.865
26.354
−13.955
1.00
32.96
C


ATOM
15337
O
GLN
B
421
38.269
25.910
−14.937
1.00
33.31
O


ATOM
15339
N
LYS
B
422
38.935
27.658
−13.686
1.00
32.66
N


ATOM
15340
CA
LYS
B
422
38.340
28.655
−14.573
1.00
32.68
C


ATOM
15342
CB
LYS
B
422
37.799
29.841
−13.768
1.00
33.55
C


ATOM
15345
CG
LYS
B
422
36.676
29.502
−12.786
1.00
36.94
C


ATOM
15348
CD
LYS
B
422
36.399
30.688
−11.850
1.00
42.73
C


ATOM
15351
CE
LYS
B
422
35.518
30.302
−10.653
1.00
46.86
C


ATOM
15354
NZ
LYS
B
422
35.616
31.295
−9.523
1.00
46.07
N


ATOM
15358
C
LYS
B
422
39.350
29.151
−15.606
1.00
32.09
C


ATOM
15359
O
LYS
B
422
39.128
30.168
−16.254
1.00
31.90
O


ATOM
15361
N
TYR
B
423
40.461
28.435
−15.758
1.00
32.07
N


ATOM
15362
CA
TYR
B
423
41.509
28.796
−16.719
1.00
32.08
C


ATOM
15364
CB
TYR
B
423
41.056
28.540
−18.159
1.00
32.37
C


ATOM
15367
CG
TYR
B
423
40.711
27.106
−18.444
1.00
34.30
C


ATOM
15368
CD1
TYR
B
423
41.698
26.182
−18.739
1.00
35.24
C


ATOM
15370
CE1
TYR
B
423
41.383
24.866
−19.005
1.00
38.80
C


ATOM
15372
CZ
TYR
B
423
40.059
24.460
−18.982
1.00
39.78
C


ATOM
15373
OH
TYR
B
423
39.732
23.153
−19.244
1.00
43.38
O


ATOM
15375
CE2
TYR
B
423
39.061
25.361
−18.697
1.00
39.46
C


ATOM
15377
CD2
TYR
B
423
39.389
26.677
−18.430
1.00
39.47
C


ATOM
15379
C
TYR
B
423
41.963
30.242
−16.576
1.00
30.88
C


ATOM
15380
O
TYR
B
423
41.962
31.003
−17.542
1.00
31.01
O


ATOM
15382
N
HIS
B
424
42.365
30.610
−15.366
1.00
30.24
N


ATOM
15383
CA
HIS
B
424
42.907
31.939
−15.112
1.00
29.00
C


ATOM
15385
CB
HIS
B
424
43.327
32.066
−13.646
1.00
28.70
C


ATOM
15388
CG
HIS
B
424
43.837
33.423
−13.284
1.00
32.16
C


ATOM
15389
ND1
HIS
B
424
45.181
33.717
−13.210
1.00
31.41
N


ATOM
15391
CE1
HIS
B
424
45.333
34.988
−12.888
1.00
33.21
C


ATOM
15393
NE2
HIS
B
424
44.137
35.530
−12.752
1.00
33.13
N


ATOM
15395
CD2
HIS
B
424
43.183
34.575
−13.003
1.00
33.86
C


ATOM
15397
C
HIS
B
424
44.091
32.221
−16.035
1.00
27.68
C


ATOM
15398
O
HIS
B
424
44.805
31.315
−16.439
1.00
27.22
O


ATOM
15400
N
ASP
B
425
44.288
33.493
−16.358
1.00
27.93
N


ATOM
15401
CA
ASP
B
425
45.369
33.932
−17.250
1.00
27.06
C


ATOM
15403
CB
ASP
B
425
45.588
35.441
−17.108
1.00
27.55
C


ATOM
15406
CG
ASP
B
425
44.468
36.259
−17.703
1.00
29.33
C


ATOM
15407
OD1
ASP
B
425
43.702
35.740
−18.547
1.00
34.93
O


ATOM
15408
OD2
ASP
B
425
44.362
37.440
−17.324
1.00
35.04
O


ATOM
15409
C
ASP
B
425
46.700
33.243
−17.017
1.00
24.99
C


ATOM
15410
O
ASP
B
425
47.443
32.998
−17.954
1.00
26.19
O


ATOM
15412
N
THR
B
426
47.005
32.956
−15.761
1.00
24.27
N


ATOM
15413
CA
THR
B
426
48.288
32.380
−15.379
1.00
23.04
C


ATOM
15415
CB
THR
B
426
48.342
32.107
−13.861
1.00
22.71
C


ATOM
15417
OG1
THR
B
426
48.157
33.339
−13.150
1.00
26.44
O


ATOM
15419
CG2
THR
B
426
49.665
31.504
−13.468
1.00
20.26
C


ATOM
15423
C
THR
B
426
48.588
31.107
−16.154
1.00
21.99
C


ATOM
15424
O
THR
B
426
49.718
30.910
−16.583
1.00
22.57
O


ATOM
15426
N
ILE
B
427
47.585
30.253
−16.343
1.00
21.32
N


ATOM
15427
CA
ILE
B
427
47.768
29.044
−17.147
1.00
21.60
C


ATOM
15429
CB
ILE
B
427
47.187
27.788
−16.449
1.00
21.56
C


ATOM
15431
CG1
ILE
B
427
45.659
27.799
−16.420
1.00
21.85
C


ATOM
15434
CD1
ILE
B
427
45.077
26.512
−15.877
1.00
18.12
C


ATOM
15438
CG2
ILE
B
427
47.720
27.677
−15.021
1.00
20.49
C


ATOM
15442
C
ILE
B
427
47.244
29.185
−18.596
1.00
22.63
C


ATOM
15443
O
ILE
B
427
47.764
28.523
−19.511
1.00
23.44
O


ATOM
15445
N
SER
B
428
46.257
30.055
−18.828
1.00
21.73
N


ATOM
15446
CA
SER
B
428
45.691
30.185
−20.178
1.00
21.85
C


ATOM
15448
CB
SER
B
428
44.376
30.959
−20.174
1.00
21.48
C


ATOM
15451
OG
SER
B
428
44.582
32.335
−19.958
1.00
22.89
O


ATOM
15453
C
SER
B
428
46.667
30.818
−21.158
1.00
21.72
C


ATOM
15454
O
SER
B
428
46.800
30.360
−22.285
1.00
21.98
O


ATOM
15456
N
ARG
B
429
47.359
31.865
−20.731
1.00
21.99
N


ATOM
15457
CA
ARG
B
429
48.264
32.576
−21.634
1.00
22.80
C


ATOM
15459
CB
ARG
B
429
48.783
33.875
−21.005
1.00
23.65
C


ATOM
15462
CG
ARG
B
429
48.145
35.141
−21.575
1.00
29.02
C


ATOM
15465
CD
ARG
B
429
47.172
35.807
−20.639
1.00
38.00
C


ATOM
15468
NE
ARG
B
429
45.769
35.651
−21.032
1.00
44.68
N


ATOM
15470
CZ
ARG
B
429
45.137
36.391
−21.947
1.00
45.10
C


ATOM
15471
NH1
ARG
B
429
45.778
37.332
−22.623
1.00
43.52
N


ATOM
15474
NH2
ARG
B
429
43.853
36.169
−22.204
1.00
46.57
N


ATOM
15477
C
ARG
B
429
49.426
31.725
−22.145
1.00
21.92
C


ATOM
15478
O
ARG
B
429
49.659
31.680
−23.348
1.00
21.67
O


ATOM
15480
N
PRO
B
430
50.159
31.049
−21.240
1.00
21.35
N


ATOM
15481
CA
PRO
B
430
51.228
30.146
−21.692
1.00
20.56
C


ATOM
15483
CB
PRO
B
430
51.730
29.495
−20.405
1.00
20.61
C


ATOM
15486
CG
PRO
B
430
51.221
30.310
−19.299
1.00
22.82
C


ATOM
15489
CD
PRO
B
430
50.072
31.130
−19.775
1.00
21.56
C


ATOM
15492
C
PRO
B
430
50.715
29.064
−22.627
1.00
19.69
C


ATOM
15493
O
PRO
B
430
51.420
28.630
−23.543
1.00
17.76
O


ATOM
15494
N
SER
B
431
49.486
28.630
−22.367
1.00
20.10
N


ATOM
15495
CA
SER
B
431
48.818
27.637
−23.190
1.00
19.87
C


ATOM
15497
CB
SER
B
431
47.523
27.185
−22.508
1.00
20.63
C


ATOM
15500
OG
SER
B
431
47.810
26.550
−21.265
1.00
20.83
O


ATOM
15502
C
SER
B
431
48.552
28.166
−24.601
1.00
19.02
C


ATOM
15503
O
SER
B
431
48.728
27.425
−25.574
1.00
18.43
O


ATOM
15505
N
HIS
B
432
48.157
29.441
−24.705
1.00
17.73
N


ATOM
15506
CA
HIS
B
432
48.016
30.115
−25.999
1.00
17.66
C


ATOM
15508
CB
HIS
B
432
47.720
31.612
−25.842
1.00
18.48
C


ATOM
15511
CG
HIS
B
432
46.387
31.922
−25.242
1.00
21.18
C


ATOM
15512
ND1
HIS
B
432
45.569
30.959
−24.693
1.00
29.61
N


ATOM
15514
CE1
HIS
B
432
44.471
31.529
−24.230
1.00
28.07
C


ATOM
15516
NE2
HIS
B
432
44.558
32.827
−24.442
1.00
24.46
N


ATOM
15518
CD2
HIS
B
432
45.747
33.100
−25.071
1.00
23.89
C


ATOM
15520
C
HIS
B
432
49.297
30.003
−26.801
1.00
17.43
C


ATOM
15521
O
HIS
B
432
49.256
29.672
−27.973
1.00
16.84
O


ATOM
15523
N
ILE
B
433
50.427
30.303
−26.158
1.00
18.30
N


ATOM
15524
CA
ILE
B
433
51.743
30.309
−26.817
1.00
19.13
C


ATOM
15526
CB
ILE
B
433
52.859
30.822
−25.874
1.00
19.72
C


ATOM
15528
CG1
ILE
B
433
52.601
32.263
−25.414
1.00
22.36
C


ATOM
15531
CD1
ILE
B
433
52.455
33.237
−26.513
1.00
24.58
C


ATOM
15535
CG2
ILE
B
433
54.225
30.718
−26.536
1.00
17.43
C


ATOM
15539
C
ILE
B
433
52.139
28.898
−27.252
1.00
20.08
C


ATOM
15540
O
ILE
B
433
52.630
28.686
−28.366
1.00
20.63
O


ATOM
15542
N
PHE
B
434
51.942
27.945
−26.347
1.00
19.69
N


ATOM
15543
CA
PHE
B
434
52.188
26.534
−26.617
1.00
19.00
C


ATOM
15545
CB
PHE
B
434
51.711
25.736
−25.398
1.00
19.70
C


ATOM
15548
CG
PHE
B
434
51.864
24.238
−25.500
1.00
22.29
C


ATOM
15549
CD1
PHE
B
434
52.639
23.624
−26.477
1.00
21.87
C


ATOM
15551
CE1
PHE
B
434
52.756
22.239
−26.519
1.00
22.23
C


ATOM
15553
CZ
PHE
B
434
52.116
21.456
−25.569
1.00
20.94
C


ATOM
15555
CE2
PHE
B
434
51.357
22.054
−24.582
1.00
24.34
C


ATOM
15557
CD2
PHE
B
434
51.233
23.432
−24.549
1.00
24.47
C


ATOM
15559
C
PHE
B
434
51.474
26.130
−27.899
1.00
18.88
C


ATOM
15560
O
PHE
B
434
52.104
25.644
−28.840
1.00
19.79
O


ATOM
15562
N
ARG
B
435
50.171
26.377
−27.962
1.00
18.36
N


ATOM
15563
CA
ARG
B
435
49.401
25.989
−29.133
1.00
18.31
C


ATOM
15565
CB
ARG
B
435
47.905
26.221
−28.907
1.00
18.44
C


ATOM
15568
CG
ARG
B
435
47.030
26.060
−30.162
1.00
18.66
C


ATOM
15571
CD
ARG
B
435
47.243
24.735
−30.870
1.00
20.84
C


ATOM
15574
NE
ARG
B
435
46.750
23.601
−30.095
1.00
20.54
N


ATOM
15576
CZ
ARG
B
435
46.962
22.327
−30.410
1.00
18.63
C


ATOM
15577
NH1
ARG
B
435
47.681
22.001
−31.481
1.00
15.31
N


ATOM
15580
NH2
ARG
B
435
46.464
21.370
−29.634
1.00
19.18
N


ATOM
15583
C
ARG
B
435
49.870
26.693
−30.409
1.00
18.19
C


ATOM
15584
O
ARG
B
435
49.975
26.058
−31.456
1.00
18.26
O


ATOM
15586
N
LEU
B
436
50.149
27.991
−30.319
1.00
18.18
N


ATOM
15587
CA
LEU
B
436
50.529
28.778
−31.498
1.00
18.60
C


ATOM
15589
CB
LEU
B
436
50.426
30.279
−31.194
1.00
18.65
C


ATOM
15592
CG
LEU
B
436
49.013
30.828
−30.933
1.00
18.58
C


ATOM
15594
CD1
LEU
B
436
49.070
32.272
−30.443
1.00
14.53
C


ATOM
15598
CD2
LEU
B
436
48.128
30.715
−32.167
1.00
12.32
C


ATOM
15602
C
LEU
B
436
51.932
28.431
−32.028
1.00
19.03
C


ATOM
15603
O
LEU
B
436
52.153
28.400
−33.232
1.00
19.41
O


ATOM
15605
N
CYS
B
437
52.875
28.171
−31.131
1.00
20.14
N


ATOM
15606
CA
CYS
B
437
54.214
27.732
−31.532
1.00
20.76
C


ATOM
15608
CB
CYS
B
437
55.127
27.622
−30.318
1.00
20.35
C


ATOM
15611
SG
CYS
B
437
55.604
29.223
−29.661
1.00
22.24
S


ATOM
15613
C
CYS
B
437
54.174
26.395
−32.258
1.00
21.65
C


ATOM
15614
O
CYS
B
437
54.779
26.239
−33.323
1.00
21.72
O


ATOM
15616
N
ASN
B
438
53.457
25.440
−31.671
1.00
22.25
N


ATOM
15617
CA
ASN
B
438
53.293
24.116
−32.255
1.00
22.51
C


ATOM
15619
CB
ASN
B
438
52.428
23.246
−31.332
1.00
23.13
C


ATOM
15622
CG
ASN
B
438
52.157
21.859
−31.895
1.00
22.55
C


ATOM
15623
OD1
ASN
B
438
52.742
21.440
−32.885
1.00
31.37
O


ATOM
15624
ND2
ASN
B
438
51.270
21.139
−31.248
1.00
23.98
N


ATOM
15627
C
ASN
B
438
52.672
24.212
−33.640
1.00
23.19
C


ATOM
15628
O
ASN
B
438
53.166
23.597
−34.597
1.00
24.74
O


ATOM
15630
N
ASP
B
439
51.600
24.991
−33.753
1.00
22.68
N


ATOM
15631
CA
ASP
B
439
50.900
25.125
−35.030
1.00
22.77
C


ATOM
15633
CB
ASP
B
439
49.510
25.726
−34.836
1.00
22.55
C


ATOM
15636
CG
ASP
B
439
48.531
24.723
−34.262
1.00
21.72
C


ATOM
15637
OD1
ASP
B
439
48.964
23.857
−33.476
1.00
22.53
O


ATOM
15638
OD2
ASP
B
439
47.336
24.791
−34.603
1.00
23.74
O


ATOM
15639
C
ASP
B
439
51.710
25.903
−36.053
1.00
22.77
C


ATOM
15640
O
ASP
B
439
51.631
25.613
−37.244
1.00
22.42
O


ATOM
15642
N
LEU
B
440
52.498
26.871
−35.589
1.00
23.21
N


ATOM
15643
CA
LEU
B
440
53.408
27.605
−36.471
1.00
23.98
C


ATOM
15645
CB
LEU
B
440
54.140
28.709
−35.705
1.00
23.92
C


ATOM
15648
CG
LEU
B
440
53.480
30.084
−35.649
1.00
23.77
C


ATOM
15650
CD1
LEU
B
440
54.222
30.982
−34.676
1.00
27.08
C


ATOM
15654
CD2
LEU
B
440
53.471
30.712
−37.016
1.00
22.99
C


ATOM
15658
C
LEU
B
440
54.436
26.679
−37.140
1.00
24.88
C


ATOM
15659
O
LEU
B
440
54.763
26.853
−38.316
1.00
25.63
O


ATOM
15661
N
ALA
B
441
54.932
25.698
−36.387
1.00
24.62
N


ATOM
15662
CA
ALA
B
441
55.981
24.793
−36.862
1.00
24.00
C


ATOM
15664
CB
ALA
B
441
56.486
23.931
−35.709
1.00
23.61
C


ATOM
15668
C
ALA
B
441
55.515
23.907
−38.011
1.00
23.92
C


ATOM
15669
O
ALA
B
441
56.243
23.696
−38.974
1.00
24.43
O


ATOM
15671
N
SER
B
442
54.295
23.398
−37.904
1.00
24.56
N


ATOM
15672
CA
SER
B
442
53.730
22.507
−38.915
1.00
24.82
C


ATOM
15674
CB
SER
B
442
52.907
21.418
−38.218
1.00
25.09
C


ATOM
15677
OG
SER
B
442
51.899
21.997
−37.398
1.00
29.33
O


ATOM
15679
C
SER
B
442
52.850
23.239
−39.944
1.00
24.46
C


ATOM
15680
O
SER
B
442
52.336
22.613
−40.882
1.00
24.20
O


ATOM
15682
N
ALA
B
443
52.692
24.555
−39.773
1.00
23.79
N


ATOM
15683
CA
ALA
B
443
51.751
25.352
−40.561
1.00
22.33
C


ATOM
15685
CB
ALA
B
443
51.939
26.827
−40.264
1.00
21.29
C


ATOM
15689
C
ALA
B
443
51.876
25.112
−42.054
1.00
22.54
C


ATOM
15690
O
ALA
B
443
50.948
24.616
−42.689
1.00
22.37
O


ATOM
15692
N
SER
B
444
53.034
25.449
−42.607
1.00
23.67
N


ATOM
15693
CA
SER
B
444
53.186
25.505
−44.053
1.00
24.77
C


ATOM
15695
CB
SER
B
444
54.483
26.227
−44.438
1.00
24.33
C


ATOM
15698
OG
SER
B
444
55.600
25.382
−44.310
1.00
29.42
O


ATOM
15700
C
SER
B
444
53.091
24.122
−44.704
1.00
25.09
C


ATOM
15701
O
SER
B
444
52.620
24.001
−45.843
1.00
25.49
O


ATOM
15703
N
ALA
B
445
53.510
23.086
−43.980
1.00
25.41
N


ATOM
15704
CA
ALA
B
445
53.411
21.717
−44.482
1.00
26.23
C


ATOM
15706
CB
ALA
B
445
54.244
20.774
−43.640
1.00
25.83
C


ATOM
15710
C
ALA
B
445
51.952
21.253
−44.535
1.00
27.75
C


ATOM
15711
O
ALA
B
445
51.514
20.694
−45.547
1.00
28.82
O


ATOM
15713
N
GLU
B
446
51.200
21.496
−43.461
1.00
28.44
N


ATOM
15714
CA
GLU
B
446
49.766
21.168
−43.435
1.00
29.32
C


ATOM
15716
CB
GLU
B
446
49.205
21.244
−42.007
1.00
29.63
C


ATOM
15719
CG
GLU
B
446
49.636
20.080
−41.125
1.00
32.37
C


ATOM
15722
CD
GLU
B
446
49.175
20.209
−39.680
1.00
39.10
C


ATOM
15723
OE1
GLU
B
446
48.071
20.744
−39.438
1.00
45.26
O


ATOM
15724
OE2
GLU
B
446
49.913
19.758
−38.777
1.00
40.42
O


ATOM
15725
C
GLU
B
446
48.932
22.043
−44.389
1.00
29.18
C


ATOM
15726
O
GLU
B
446
47.978
21.560
−44.997
1.00
28.80
O


ATOM
15728
N
ILE
B
447
49.290
23.317
−44.524
1.00
29.38
N


ATOM
15729
CA
ILE
B
447
48.598
24.208
−45.462
1.00
29.77
C


ATOM
15731
CB
ILE
B
447
49.071
25.676
−45.312
1.00
29.91
C


ATOM
15733
CG1
ILE
B
447
48.562
26.259
−43.990
1.00
30.26
C


ATOM
15736
CD1
ILE
B
447
49.269
27.519
−43.566
1.00
29.22
C


ATOM
15740
CG2
ILE
B
447
48.583
26.534
−46.475
1.00
25.60
C


ATOM
15744
C
ILE
B
447
48.784
23.747
−46.907
1.00
31.06
C


ATOM
15745
O
ILE
B
447
47.897
23.927
−47.734
1.00
31.19
O


ATOM
15747
N
ALA
B
448
49.937
23.152
−47.200
1.00
32.75
N


ATOM
15748
CA
ALA
B
448
50.216
22.607
−48.526
1.00
33.94
C


ATOM
15750
CB
ALA
B
448
51.694
22.279
−48.654
1.00
34.47
C


ATOM
15754
C
ALA
B
448
49.367
21.366
−48.825
1.00
35.48
C


ATOM
15755
O
ALA
B
448
48.876
21.217
−49.939
1.00
36.06
O


ATOM
15757
N
ARG
B
449
49.197
20.486
−47.835
1.00
37.10
N


ATOM
15758
CA
ARG
B
449
48.324
19.305
−47.969
1.00
38.26
C


ATOM
15760
CB
ARG
B
449
48.489
18.339
−46.787
1.00
39.28
C


ATOM
15763
CG
ARG
B
449
49.867
17.725
−46.625
1.00
44.41
C


ATOM
15766
CD
ARG
B
449
49.858
16.573
−45.603
1.00
48.39
C


ATOM
15769
NE
ARG
B
449
50.939
16.714
−44.620
1.00
50.95
N


ATOM
15771
CZ
ARG
B
449
50.781
16.813
−43.297
1.00
50.45
C


ATOM
15772
NH1
ARG
B
449
49.579
16.749
−42.733
1.00
48.57
N


ATOM
15775
NH2
ARG
B
449
51.851
16.956
−42.520
1.00
50.04
N


ATOM
15778
C
ARG
B
449
46.848
19.668
−48.040
1.00
37.74
C


ATOM
15779
O
ARG
B
449
46.030
18.837
−48.418
1.00
38.10
O


ATOM
15781
N
GLY
B
450
46.506
20.887
−47.635
1.00
37.69
N


ATOM
15782
CA
GLY
B
450
45.124
21.352
−47.653
1.00
37.69
C


ATOM
15785
C
GLY
B
450
44.357
20.983
−46.396
1.00
37.78
C


ATOM
15786
O
GLY
B
450
43.141
20.809
−46.446
1.00
38.32
O


ATOM
15788
N
GLU
B
451
45.060
20.866
−45.267
1.00
37.11
N


ATOM
15789
CA
GLU
B
451
44.413
20.592
−43.983
1.00
36.64
C


ATOM
15791
CB
GLU
B
451
45.261
19.660
−43.111
1.00
37.70
C


ATOM
15794
CG
GLU
B
451
45.064
18.181
−43.442
1.00
41.62
C


ATOM
15797
CD
GLU
B
451
46.092
17.283
−42.775
1.00
46.25
C


ATOM
15798
OE1
GLU
B
451
46.040
17.121
−41.532
1.00
47.91
O


ATOM
15799
OE2
GLU
B
451
46.948
16.732
−43.501
1.00
49.29
O


ATOM
15800
C
GLU
B
451
44.115
21.891
−43.248
1.00
34.73
C


ATOM
15801
O
GLU
B
451
44.908
22.834
−43.274
1.00
34.16
O


ATOM
15803
N
THR
B
452
42.964
21.909
−42.583
1.00
32.80
N


ATOM
15804
CA
THR
B
452
42.411
23.115
−41.989
1.00
31.18
C


ATOM
15806
CB
THR
B
452
40.911
23.255
−42.355
1.00
31.64
C


ATOM
15808
OG1
THR
B
452
40.165
22.173
−41.791
1.00
32.65
O


ATOM
15810
CG2
THR
B
452
40.730
23.277
−43.878
1.00
29.23
C


ATOM
15814
C
THR
B
452
42.573
23.174
−40.469
1.00
29.72
C


ATOM
15815
O
THR
B
452
42.567
24.259
−39.893
1.00
29.47
O


ATOM
15817
N
ALA
B
453
42.718
22.020
−39.819
1.00
28.62
N


ATOM
15818
CA
ALA
B
453
42.876
21.971
−38.364
1.00
27.16
C


ATOM
15820
CB
ALA
B
453
42.593
20.573
−37.853
1.00
26.02
C


ATOM
15824
C
ALA
B
453
44.275
22.443
−37.929
1.00
26.32
C


ATOM
15825
O
ALA
B
453
45.119
21.646
−37.530
1.00
26.61
O


ATOM
15827
N
ASN
B
454
44.497
23.751
−38.000
1.00
24.18
N


ATOM
15828
CA
ASN
B
454
45.764
24.357
−37.606
1.00
23.29
C


ATOM
15830
CB
ASN
B
454
46.843
24.077
−38.665
1.00
22.87
C


ATOM
15833
CG
ASN
B
454
48.181
24.741
−38.349
1.00
22.94
C


ATOM
15834
OD1
ASN
B
454
48.369
25.930
−38.602
1.00
24.63
O


ATOM
15835
ND2
ASN
B
454
49.127
23.962
−37.827
1.00
21.89
N


ATOM
15838
C
ASN
B
454
45.542
25.859
−37.404
1.00
21.96
C


ATOM
15839
O
ASN
B
454
45.028
26.541
−38.287
1.00
22.37
O


ATOM
15841
N
SER
B
455
45.922
26.369
−36.242
1.00
20.32
N


ATOM
15842
CA
SER
B
455
45.627
27.753
−35.870
1.00
19.88
C


ATOM
15844
CB
SER
B
455
46.399
28.121
−34.609
1.00
19.55
C


ATOM
15847
OG
SER
B
455
45.987
27.295
−33.539
1.00
19.87
O


ATOM
15849
C
SER
B
455
45.937
28.762
−36.975
1.00
19.31
C


ATOM
15850
O
SER
B
455
45.166
29.696
−37.210
1.00
18.96
O


ATOM
15852
N
VAL
B
456
47.059
28.563
−37.657
1.00
18.38
N


ATOM
15853
CA
VAL
B
456
47.493
29.488
−38.689
1.00
17.04
C


ATOM
15855
CB
VAL
B
456
48.949
29.247
−39.087
1.00
17.08
C


ATOM
15857
CG1
VAL
B
456
49.373
30.233
−40.169
1.00
13.96
C


ATOM
15861
CG2
VAL
B
456
49.839
29.366
−37.870
1.00
13.64
C


ATOM
15865
C
VAL
B
456
46.624
29.343
−39.905
1.00
17.23
C


ATOM
15866
O
VAL
B
456
46.222
30.331
−40.501
1.00
18.52
O


ATOM
15868
N
SER
B
457
46.338
28.101
−40.271
1.00
17.87
N


ATOM
15869
CA
SER
B
457
45.474
27.813
−41.410
1.00
17.55
C


ATOM
15871
CB
SER
B
457
45.343
26.303
−41.598
1.00
17.69
C


ATOM
15874
OG
SER
B
457
44.401
26.015
−42.611
1.00
17.14
O


ATOM
15876
C
SER
B
457
44.095
28.399
−41.196
1.00
17.12
C


ATOM
15877
O
SER
B
457
43.455
28.863
−42.120
1.00
16.99
O


ATOM
15879
N
CYS
B
458
43.652
28.358
−39.951
1.00
18.30
N


ATOM
15880
CA
CYS
B
458
42.337
28.823
−39.568
1.00
18.96
C


ATOM
15882
CB
CYS
B
458
42.030
28.310
−38.159
1.00
19.74
C


ATOM
15885
SG
CYS
B
458
40.316
28.094
−37.856
1.00
21.97
S


ATOM
15887
C
CYS
B
458
42.280
30.345
−39.602
1.00
17.64
C


ATOM
15888
O
CYS
B
458
41.287
30.930
−40.011
1.00
17.38
O


ATOM
15890
N
TYR
B
459
43.356
30.978
−39.157
1.00
17.81
N


ATOM
15891
CA
TYR
B
459
43.463
32.427
−39.203
1.00
18.66
C


ATOM
15893
CB
TYR
B
459
44.752
32.888
−38.512
1.00
18.82
C


ATOM
15896
CG
TYR
B
459
44.745
34.329
−38.046
1.00
19.88
C


ATOM
15897
CD1
TYR
B
459
45.002
35.367
−38.936
1.00
21.14
C


ATOM
15899
CE1
TYR
B
459
45.008
36.692
−38.516
1.00
22.60
C


ATOM
15901
CZ
TYR
B
459
44.764
36.990
−37.190
1.00
22.72
C


ATOM
15902
OH
TYR
B
459
44.771
38.304
−36.790
1.00
30.20
O


ATOM
15904
CE2
TYR
B
459
44.510
35.979
−36.279
1.00
20.11
C


ATOM
15906
CD2
TYR
B
459
44.506
34.653
−36.708
1.00
20.19
C


ATOM
15908
C
TYR
B
459
43.410
32.903
−40.658
1.00
18.23
C


ATOM
15909
O
TYR
B
459
42.671
33.825
−40.969
1.00
17.86
O


ATOM
15911
N
MET
B
460
44.171
32.257
−41.542
1.00
18.77
N


ATOM
15912
CA
MET
B
460
44.127
32.549
−42.983
1.00
19.43
C


ATOM
15914
CB
MET
B
460
44.895
31.502
−43.787
1.00
20.18
C


ATOM
15917
CG
MET
B
460
46.397
31.555
−43.697
1.00
22.86
C


ATOM
15920
SD
MET
B
460
47.095
30.222
−44.688
1.00
27.17
S


ATOM
15921
CE
MET
B
460
46.492
30.645
−46.323
1.00
13.86
C


ATOM
15925
C
MET
B
460
42.720
32.516
−43.522
1.00
19.28
C


ATOM
15926
O
MET
B
460
42.278
33.450
−44.185
1.00
19.45
O


ATOM
15928
N
ARG
B
461
42.032
31.410
−43.259
1.00
19.47
N


ATOM
15929
CA
ARG
B
461
40.690
31.192
−43.784
1.00
20.04
C


ATOM
15931
CB
ARG
B
461
40.285
29.733
−43.598
1.00
20.96
C


ATOM
15934
CG
ARG
B
461
38.857
29.383
−44.071
1.00
30.90
C


ATOM
15937
CD
ARG
B
461
38.546
27.881
−43.927
1.00
38.84
C


ATOM
15940
NE
ARG
B
461
39.169
27.321
−42.724
1.00
45.44
N


ATOM
15942
CZ
ARG
B
461
40.371
26.735
−42.677
1.00
49.44
C


ATOM
15943
NH1
ARG
B
461
40.841
26.292
−41.509
1.00
45.70
N


ATOM
15946
NH2
ARG
B
461
41.110
26.591
−43.782
1.00
49.92
N


ATOM
15949
C
ARG
B
461
39.666
32.116
−43.135
1.00
17.59
C


ATOM
15950
O
ARG
B
461
38.758
32.586
−43.805
1.00
17.29
O


ATOM
15952
N
THR
B
462
39.813
32.370
−41.837
1.00
16.54
N


ATOM
15953
CA
THR
B
462
38.921
33.284
−41.126
1.00
15.49
C


ATOM
15955
CB
THR
B
462
39.222
33.309
−39.609
1.00
15.13
C


ATOM
15957
OG1
THR
B
462
38.948
32.021
−39.058
1.00
15.06
O


ATOM
15959
CG2
THR
B
462
38.371
34.337
−38.879
1.00
9.62
C


ATOM
15963
C
THR
B
462
39.024
34.687
−41.711
1.00
15.44
C


ATOM
15964
O
THR
B
462
38.003
35.316
−41.987
1.00
16.12
O


ATOM
15966
N
LYS
B
463
40.256
35.146
−41.929
1.00
14.96
N


ATOM
15967
CA
LYS
B
463
40.526
36.494
−42.431
1.00
15.15
C


ATOM
15969
CB
LYS
B
463
41.889
36.976
−41.930
1.00
15.42
C


ATOM
15972
CG
LYS
B
463
42.000
37.194
−40.417
1.00
19.93
C


ATOM
15975
CD
LYS
B
463
40.751
37.850
−39.816
1.00
24.60
C


ATOM
15978
CE
LYS
B
463
41.060
38.829
−38.699
1.00
26.69
C


ATOM
15981
NZ
LYS
B
463
40.981
40.225
−39.225
1.00
24.76
N


ATOM
15985
C
LYS
B
463
40.502
36.639
−43.951
1.00
14.57
C


ATOM
15986
O
LYS
B
463
40.312
37.743
−44.462
1.00
14.03
O


ATOM
15988
N
GLY
B
464
40.712
35.539
−44.667
1.00
13.99
N


ATOM
15989
CA
GLY
B
464
40.841
35.580
−46.120
1.00
13.37
C


ATOM
15992
C
GLY
B
464
42.142
36.231
−46.542
1.00
13.27
C


ATOM
15993
O
GLY
B
464
42.172
37.025
−47.471
1.00
13.56
O


ATOM
15995
N
ILE
B
465
43.221
35.898
−45.847
1.00
13.64
N


ATOM
15996
CA
ILE
B
465
44.529
36.476
−46.127
1.00
15.05
C


ATOM
15998
CB
ILE
B
465
45.049
37.332
−44.935
1.00
15.19
C


ATOM
16000
CG1
ILE
B
465
45.365
36.451
−43.710
1.00
16.18
C


ATOM
16003
CD1
ILE
B
465
45.741
37.224
−42.451
1.00
13.60
C


ATOM
16007
CG2
ILE
B
465
44.042
38.419
−44.592
1.00
11.63
C


ATOM
16011
C
ILE
B
465
45.531
35.375
−46.451
1.00
16.34
C


ATOM
16012
O
ILE
B
465
45.257
34.188
−46.258
1.00
16.93
O


ATOM
16014
N
SER
B
466
46.700
35.779
−46.934
1.00
17.84
N


ATOM
16015
CA
SER
B
466
47.764
34.835
−47.268
1.00
18.68
C


ATOM
16017
CB
SER
B
466
48.867
35.535
−48.057
1.00
18.26
C


ATOM
16020
OG
SER
B
466
49.572
36.448
−47.233
1.00
18.06
O


ATOM
16022
C
SER
B
466
48.376
34.202
−46.022
1.00
20.07
C


ATOM
16023
O
SER
B
466
48.154
34.652
−44.894
1.00
20.37
O


ATOM
16025
N
GLU
B
467
49.169
33.161
−46.246
1.00
21.18
N


ATOM
16026
CA
GLU
B
467
49.906
32.507
−45.176
1.00
21.39
C


ATOM
16028
CB
GLU
B
467
50.611
31.260
−45.707
1.00
21.20
C


ATOM
16031
CG
GLU
B
467
51.204
30.391
−44.621
1.00
23.32
C


ATOM
16034
CD
GLU
B
467
52.011
29.225
−45.150
1.00
27.21
C


ATOM
16035
OE1
GLU
B
467
51.874
28.868
−46.343
1.00
30.36
O


ATOM
16036
OE2
GLU
B
467
52.790
28.663
−44.352
1.00
30.37
O


ATOM
16037
C
GLU
B
467
50.925
33.444
−44.527
1.00
22.15
C


ATOM
16038
O
GLU
B
467
51.182
33.347
−43.330
1.00
23.83
O


ATOM
16040
N
GLU
B
468
51.506
34.346
−45.310
1.00
22.98
N


ATOM
16041
CA
GLU
B
468
52.544
35.236
−44.799
1.00
23.24
C


ATOM
16043
CB
GLU
B
468
53.245
35.981
−45.938
1.00
24.25
C


ATOM
16046
CG
GLU
B
468
54.555
36.643
−45.502
1.00
29.56
C


ATOM
16049
CD
GLU
B
468
55.013
37.781
−46.413
1.00
35.60
C


ATOM
16050
OE1
GLU
B
468
54.396
38.020
−47.479
1.00
36.06
O


ATOM
16051
OE2
GLU
B
468
56.009
38.442
−46.046
1.00
37.98
O


ATOM
16052
C
GLU
B
468
51.966
36.234
−43.801
1.00
22.22
C


ATOM
16053
O
GLU
B
468
52.568
36.486
−42.758
1.00
22.43
O


ATOM
16055
N
LEU
B
469
50.806
36.806
−44.118
1.00
21.75
N


ATOM
16056
CA
LEU
B
469
50.136
37.719
−43.184
1.00
21.45
C


ATOM
16058
CB
LEU
B
469
49.000
38.494
−43.858
1.00
22.36
C


ATOM
16061
CG
LEU
B
469
49.365
39.834
−44.496
1.00
27.45
C


ATOM
16063
CD1
LEU
B
469
49.466
39.726
−46.033
1.00
30.95
C


ATOM
16067
CD2
LEU
B
469
48.322
40.873
−44.095
1.00
31.35
C


ATOM
16071
C
LEU
B
469
49.596
36.969
−41.967
1.00
18.63
C


ATOM
16072
O
LEU
B
469
49.701
37.453
−40.846
1.00
18.64
O


ATOM
16074
N
ALA
B
470
49.023
35.792
−42.194
1.00
15.97
N


ATOM
16075
CA
ALA
B
470
48.534
34.959
−41.101
1.00
15.14
C


ATOM
16077
CB
ALA
B
470
47.952
33.667
−41.637
1.00
15.02
C


ATOM
16081
C
ALA
B
470
49.653
34.658
−40.118
1.00
14.59
C


ATOM
16082
O
ALA
B
470
49.449
34.702
−38.900
1.00
12.83
O


ATOM
16084
N
THR
B
471
50.835
34.364
−40.657
1.00
14.22
N


ATOM
16085
CA
THR
B
471
51.995
34.038
−39.836
1.00
14.86
C


ATOM
16087
CB
THR
B
471
53.167
33.512
−40.706
1.00
14.55
C


ATOM
16089
OG1
THR
B
471
52.731
32.360
−41.437
1.00
13.22
O


ATOM
16091
CG2
THR
B
471
54.361
33.127
−39.846
1.00
8.88
C


ATOM
16095
C
THR
B
471
52.416
35.255
−39.004
1.00
15.80
C


ATOM
16096
O
THR
B
471
52.566
35.172
−37.781
1.00
15.84
O


ATOM
16098
N
GLU
B
472
52.566
36.386
−39.677
1.00
16.48
N


ATOM
16099
CA
GLU
B
472
52.859
37.656
−39.019
1.00
17.64
C


ATOM
16101
CB
GLU
B
472
52.844
38.782
−40.061
1.00
18.33
C


ATOM
16104
CG
GLU
B
472
53.379
40.109
−39.564
1.00
25.22
C


ATOM
16107
CD
GLU
B
472
53.249
41.204
−40.605
1.00
33.82
C


ATOM
16108
OE1
GLU
B
472
53.894
41.092
−41.674
1.00
37.62
O


ATOM
16109
OE2
GLU
B
472
52.496
42.172
−40.352
1.00
36.62
O


ATOM
16110
C
GLU
B
472
51.861
37.955
−37.888
1.00
16.57
C


ATOM
16111
O
GLU
B
472
52.259
38.242
−36.762
1.00
16.22
O


ATOM
16113
N
SER
B
473
50.569
37.880
−38.201
1.00
16.11
N


ATOM
16114
CA
SER
B
473
49.511
38.127
−37.222
1.00
15.94
C


ATOM
16116
CB
SER
B
473
48.135
37.974
−37.872
1.00
16.19
C


ATOM
16119
OG
SER
B
473
47.989
38.830
−38.993
1.00
18.75
O


ATOM
16121
C
SER
B
473
49.599
37.193
−36.014
1.00
15.73
C


ATOM
16122
O
SER
B
473
49.345
37.611
−34.887
1.00
14.53
O


ATOM
16124
N
VAL
B
474
49.951
35.931
−36.253
1.00
16.02
N


ATOM
16125
CA
VAL
B
474
50.108
34.963
−35.165
1.00
16.14
C


ATOM
16127
CB
VAL
B
474
50.140
33.502
−35.684
1.00
15.37
C


ATOM
16129
CG1
VAL
B
474
50.594
32.561
−34.601
1.00
13.08
C


ATOM
16133
CG2
VAL
B
474
48.767
33.087
−36.191
1.00
11.54
C


ATOM
16137
C
VAL
B
474
51.358
35.278
−34.335
1.00
17.47
C


ATOM
16138
O
VAL
B
474
51.377
35.038
−33.130
1.00
17.05
O


ATOM
16140
N
MET
B
475
52.386
35.842
−34.962
1.00
18.50
N


ATOM
16141
CA
MET
B
475
53.573
36.251
−34.217
1.00
20.29
C


ATOM
16143
CB
MET
B
475
54.699
36.692
−35.143
1.00
22.08
C


ATOM
16146
CG
MET
B
475
55.071
35.707
−36.231
1.00
28.44
C


ATOM
16149
SD
MET
B
475
56.648
34.919
−35.969
1.00
42.67
S


ATOM
16150
CE
MET
B
475
57.367
35.163
−37.600
1.00
30.81
C


ATOM
16154
C
MET
B
475
53.218
37.417
−33.316
1.00
19.92
C


ATOM
16155
O
MET
B
475
53.605
37.442
−32.152
1.00
19.67
O


ATOM
16157
N
ASN
B
476
52.494
38.390
−33.865
1.00
20.28
N


ATOM
16158
CA
ASN
B
476
52.129
39.587
−33.107
1.00
21.78
C


ATOM
16160
CB
ASN
B
476
51.343
40.578
−33.972
1.00
22.56
C


ATOM
16163
CG
ASN
B
476
52.223
41.316
−34.981
1.00
27.45
C


ATOM
16164
OD1
ASN
B
476
53.423
41.517
−34.765
1.00
34.13
O


ATOM
16165
ND2
ASN
B
476
51.617
41.737
−36.086
1.00
32.95
N


ATOM
16168
C
ASN
B
476
51.308
39.202
−31.895
1.00
21.47
C


ATOM
16169
O
ASN
B
476
51.488
39.739
−30.802
1.00
21.39
O


ATOM
16171
N
LEU
B
477
50.426
38.236
−32.107
1.00
21.52
N


ATOM
16172
CA
LEU
B
477
49.550
37.741
−31.072
1.00
21.65
C


ATOM
16174
CB
LEU
B
477
48.575
36.737
−31.688
1.00
21.80
C


ATOM
16177
CG
LEU
B
477
47.298
36.403
−30.921
1.00
29.34
C


ATOM
16179
CD1
LEU
B
477
46.655
37.641
−30.283
1.00
31.33
C


ATOM
16183
CD2
LEU
B
477
46.323
35.697
−31.869
1.00
33.64
C


ATOM
16187
C
LEU
B
477
50.364
37.123
−29.934
1.00
21.00
C


ATOM
16188
O
LEU
B
477
50.038
37.292
−28.758
1.00
21.26
O


ATOM
16190
N
ILE
B
478
51.437
36.425
−30.285
1.00
20.88
N


ATOM
16191
CA
ILE
B
478
52.343
35.874
−29.286
1.00
20.29
C


ATOM
16193
CB
ILE
B
478
53.399
34.962
−29.912
1.00
20.06
C


ATOM
16195
CG1
ILE
B
478
52.766
33.659
−30.399
1.00
19.03
C


ATOM
16198
CD1
ILE
B
478
53.701
32.844
−31.280
1.00
18.48
C


ATOM
16202
CG2
ILE
B
478
54.495
34.670
−28.906
1.00
19.59
C


ATOM
16206
C
ILE
B
478
53.066
36.986
−28.538
1.00
20.55
C


ATOM
16207
O
ILE
B
478
53.236
36.890
−27.322
1.00
20.34
O


ATOM
16209
N
ASP
B
479
53.501
38.027
−29.256
1.00
19.49
N


ATOM
16210
CA
ASP
B
479
54.169
39.154
−28.607
1.00
20.02
C


ATOM
16212
CB
ASP
B
479
54.598
40.246
−29.606
1.00
20.61
C


ATOM
16215
CG
ASP
B
479
55.757
39.820
−30.510
1.00
21.25
C


ATOM
16216
OD1
ASP
B
479
56.768
39.298
−30.012
1.00
22.48
O


ATOM
16217
OD2
ASP
B
479
55.664
40.030
−31.737
1.00
29.16
O


ATOM
16218
C
ASP
B
479
53.227
39.755
−27.573
1.00
19.47
C


ATOM
16219
O
ASP
B
479
53.639
40.102
−26.469
1.00
18.58
O


ATOM
16221
N
GLU
B
480
51.954
39.850
−27.938
1.00
19.80
N


ATOM
16222
CA
GLU
B
480
50.950
40.490
−27.091
1.00
20.52
C


ATOM
16224
CB
GLU
B
480
49.684
40.776
−27.913
1.00
21.52
C


ATOM
16227
CG
GLU
B
480
49.022
42.120
−27.642
1.00
28.59
C


ATOM
16230
CD
GLU
B
480
49.917
43.311
−27.957
1.00
36.32
C


ATOM
16231
OE1
GLU
B
480
50.734
43.236
−28.904
1.00
39.57
O


ATOM
16232
OE2
GLU
B
480
49.802
44.330
−27.242
1.00
40.57
O


ATOM
16233
C
GLU
B
480
50.635
39.609
−25.877
1.00
19.60
C


ATOM
16234
O
GLU
B
480
50.439
40.111
−24.762
1.00
19.00
O


ATOM
16236
N
THR
B
481
50.602
38.296
−26.093
1.00
18.24
N


ATOM
16237
CA
THR
B
481
50.408
37.352
−25.001
1.00
17.14
C


ATOM
16239
CB
THR
B
481
50.282
35.920
−25.517
1.00
17.51
C


ATOM
16241
OG1
THR
B
481
49.198
35.852
−26.454
1.00
17.24
O


ATOM
16243
CG2
THR
B
481
50.038
34.943
−24.357
1.00
12.60
C


ATOM
16247
C
THR
B
481
51.560
37.436
−24.000
1.00
17.65
C


ATOM
16248
O
THR
B
481
51.330
37.380
−22.790
1.00
18.63
O


ATOM
16250
N
TRP
B
482
52.788
37.592
−24.500
1.00
16.03
N


ATOM
16251
CA
TRP
B
482
53.949
37.758
−23.623
1.00
15.61
C


ATOM
16253
CB
TRP
B
482
55.267
37.743
−24.412
1.00
16.18
C


ATOM
16256
CG
TRP
B
482
55.862
36.380
−24.503
1.00
12.24
C


ATOM
16257
CD1
TRP
B
482
55.688
35.486
−25.505
1.00
17.04
C


ATOM
16259
NE1
TRP
B
482
56.373
34.329
−25.237
1.00
18.27
N


ATOM
16261
CE2
TRP
B
482
57.006
34.460
−24.033
1.00
16.69
C


ATOM
16262
CD2
TRP
B
482
56.706
35.748
−23.542
1.00
13.90
C


ATOM
16263
CE3
TRP
B
482
57.239
36.141
−22.309
1.00
19.79
C


ATOM
16265
CZ3
TRP
B
482
58.048
35.239
−21.609
1.00
19.61
C


ATOM
16267
CH2
TRP
B
482
58.335
33.963
−22.134
1.00
19.45
C


ATOM
16269
CZ2
TRP
B
482
57.820
33.557
−23.339
1.00
16.58
C


ATOM
16271
C
TRP
B
482
53.844
39.020
−22.775
1.00
15.85
C


ATOM
16272
O
TRP
B
482
54.053
38.972
−21.566
1.00
16.02
O


ATOM
16274
N
LYS
B
483
53.508
40.143
−23.403
1.00
16.63
N


ATOM
16275
CA
LYS
B
483
53.289
41.382
−22.666
1.00
16.51
C


ATOM
16277
CB
LYS
B
483
52.685
42.459
−23.555
1.00
16.85
C


ATOM
16280
CG
LYS
B
483
53.622
43.028
−24.597
1.00
17.06
C


ATOM
16283
CD
LYS
B
483
53.017
44.249
−25.266
1.00
17.24
C


ATOM
16286
CE
LYS
B
483
53.793
44.650
−26.519
1.00
19.91
C


ATOM
16289
NZ
LYS
B
483
53.361
45.964
−27.077
1.00
17.47
N


ATOM
16293
C
LYS
B
483
52.371
41.135
−21.479
1.00
18.03
C


ATOM
16294
O
LYS
B
483
52.665
41.569
−20.367
1.00
20.06
O


ATOM
16296
N
LYS
B
484
51.275
40.415
−21.703
1.00
18.71
N


ATOM
16297
CA
LYS
B
484
50.311
40.144
−20.624
1.00
18.89
C


ATOM
16299
CB
LYS
B
484
48.990
39.626
−21.201
1.00
19.16
C


ATOM
16302
CG
LYS
B
484
48.181
40.722
−21.895
1.00
18.50
C


ATOM
16305
CD
LYS
B
484
46.984
40.151
−22.621
1.00
20.68
C


ATOM
16308
CE
LYS
B
484
46.186
41.211
−23.370
1.00
23.96
C


ATOM
16311
NZ
LYS
B
484
45.464
40.617
−24.542
1.00
28.47
N


ATOM
16315
C
LYS
B
484
50.854
39.205
−19.533
1.00
18.92
C


ATOM
16316
O
LYS
B
484
50.587
39.417
−18.352
1.00
19.71
O


ATOM
16318
N
MET
B
485
51.618
38.185
−19.926
1.00
18.18
N


ATOM
16319
CA
MET
B
485
52.302
37.315
−18.965
1.00
17.49
C


ATOM
16321
CB
MET
B
485
53.084
36.211
−19.673
1.00
17.28
C


ATOM
16324
CG
MET
B
485
52.227
35.249
−20.462
1.00
19.16
C


ATOM
16327
SD
MET
B
485
53.072
33.713
−20.826
1.00
16.74
S


ATOM
16328
CE
MET
B
485
54.442
34.310
−21.800
1.00
16.94
C


ATOM
16332
C
MET
B
485
53.280
38.111
−18.123
1.00
18.05
C


ATOM
16333
O
MET
B
485
53.380
37.899
−16.917
1.00
19.44
O


ATOM
16335
N
ASN
B
486
54.012
39.013
−18.768
1.00
17.71
N


ATOM
16336
CA
ASN
B
486
54.960
39.865
−18.071
1.00
17.79
C


ATOM
16338
CB
ASN
B
486
55.627
40.842
−19.045
1.00
18.13
C


ATOM
16341
CG
ASN
B
486
56.576
40.153
−20.024
1.00
18.10
C


ATOM
16342
OD1
ASN
B
486
57.168
39.115
−19.717
1.00
16.50
O


ATOM
16343
ND2
ASN
B
486
56.742
40.751
−21.203
1.00
9.57
N


ATOM
16346
C
ASN
B
486
54.294
40.635
−16.935
1.00
18.50
C


ATOM
16347
O
ASN
B
486
54.877
40.774
−15.859
1.00
17.83
O


ATOM
16349
N
LYS
B
487
53.072
41.117
−17.173
1.00
19.57
N


ATOM
16350
CA
LYS
B
487
52.323
41.883
−16.164
1.00
20.63
C


ATOM
16352
CB
LYS
B
487
51.088
42.531
−16.791
1.00
20.49
C


ATOM
16355
CG
LYS
B
487
50.358
43.492
−15.862
1.00
21.89
C


ATOM
16358
CD
LYS
B
487
49.409
44.401
−16.629
1.00
24.87
C


ATOM
16361
CE
LYS
B
487
48.680
45.379
−15.704
1.00
29.00
C


ATOM
16364
NZ
LYS
B
487
47.418
45.917
−16.325
1.00
28.97
N


ATOM
16368
C
LYS
B
487
51.893
41.014
−14.981
1.00
21.34
C


ATOM
16369
O
LYS
B
487
52.018
41.416
−13.822
1.00
20.66
O


ATOM
16371
N
GLU
B
488
51.384
39.827
−15.287
1.00
22.31
N


ATOM
16372
CA
GLU
B
488
50.997
38.858
−14.268
1.00
23.77
C


ATOM
16374
CB
GLU
B
488
50.461
37.592
−14.940
1.00
24.98
C


ATOM
16377
CG
GLU
B
488
49.890
36.543
−13.991
1.00
31.46
C


ATOM
16380
CD
GLU
B
488
48.678
37.032
−13.224
1.00
37.56
C


ATOM
16381
OE1
GLU
B
488
47.955
37.915
−13.739
1.00
41.02
O


ATOM
16382
OE2
GLU
B
488
48.449
36.518
−12.105
1.00
41.97
O


ATOM
16383
C
GLU
B
488
52.171
38.508
−13.345
1.00
22.92
C


ATOM
16384
O
GLU
B
488
52.000
38.420
−12.133
1.00
24.11
O


ATOM
16386
N
LYS
B
489
53.354
38.308
−13.919
1.00
21.63
N


ATOM
16387
CA
LYS
B
489
54.539
37.972
−13.133
1.00
22.22
C


ATOM
16389
CB
LYS
B
489
55.699
37.552
−14.051
1.00
21.37
C


ATOM
16392
CG
LYS
B
489
57.023
37.253
−13.338
1.00
21.61
C


ATOM
16395
CD
LYS
B
489
56.891
36.139
−12.298
1.00
23.04
C


ATOM
16398
CE
LYS
B
489
58.166
35.927
−11.489
1.00
23.96
C


ATOM
16401
NZ
LYS
B
489
59.345
35.655
−12.343
1.00
26.89
N


ATOM
16405
C
LYS
B
489
54.975
39.145
−12.257
1.00
22.89
C


ATOM
16406
O
LYS
B
489
55.447
38.951
−11.138
1.00
22.65
O


ATOM
16408
N
LEU
B
490
54.797
40.354
−12.780
1.00
24.17
N


ATOM
16409
CA
LEU
B
490
55.322
41.566
−12.174
1.00
25.54
C


ATOM
16411
CB
LEU
B
490
55.631
42.565
−13.287
1.00
25.27
C


ATOM
16414
CG
LEU
B
490
56.331
43.868
−12.950
1.00
25.47
C


ATOM
16416
CD1
LEU
B
490
57.620
43.616
−12.200
1.00
29.26
C


ATOM
16420
CD2
LEU
B
490
56.605
44.607
−14.243
1.00
27.84
C


ATOM
16424
C
LEU
B
490
54.342
42.143
−11.143
1.00
27.20
C


ATOM
16425
O
LEU
B
490
54.669
42.254
−9.970
1.00
26.26
O


ATOM
16427
N
SER
B
491
53.142
42.497
−11.592
1.00
30.29
N


ATOM
16428
CA
SER
B
491
52.039
42.867
−10.706
1.00
32.69
C


ATOM
16430
CB
SER
B
491
51.104
43.840
−11.416
1.00
32.26
C


ATOM
16433
OG
SER
B
491
51.842
44.739
−12.226
1.00
34.32
O


ATOM
16435
C
SER
B
491
51.325
41.560
−10.390
1.00
35.71
C


ATOM
16436
O
SER
B
491
51.961
40.513
−10.370
1.00
38.56
O


ATOM
16438
N
GLY
B
492
50.016
41.586
−10.163
1.00
37.35
N


ATOM
16439
CA
GLY
B
492
49.259
40.328
−10.115
1.00
38.82
C


ATOM
16442
C
GLY
B
492
49.400
39.660
−8.765
1.00
39.55
C


ATOM
16443
O
GLY
B
492
50.502
39.521
−8.243
1.00
39.41
O


ATOM
16445
N
SER
B
493
48.274
39.227
−8.211
1.00
41.09
N


ATOM
16446
CA
SER
B
493
48.170
38.994
−6.772
1.00
41.98
C


ATOM
16448
CB
SER
B
493
47.344
40.129
−6.155
1.00
41.97
C


ATOM
16451
OG
SER
B
493
46.005
40.082
−6.622
1.00
41.03
O


ATOM
16453
C
SER
B
493
47.532
37.669
−6.365
1.00
42.25
C


ATOM
16454
O
SER
B
493
47.214
37.486
−5.187
1.00
43.88
O


ATOM
16456
N
LEU
B
494
47.336
36.748
−7.302
1.00
41.48
N


ATOM
16457
CA
LEU
B
494
46.654
35.509
−6.960
1.00
41.11
C


ATOM
16459
CB
LEU
B
494
45.665
35.113
−8.052
1.00
42.32
C


ATOM
16462
CG
LEU
B
494
44.604
34.100
−7.610
1.00
46.31
C


ATOM
16464
CD1
LEU
B
494
43.966
34.473
−6.272
1.00
50.47
C


ATOM
16468
CD2
LEU
B
494
43.549
33.969
−8.685
1.00
49.74
C


ATOM
16472
C
LEU
B
494
47.635
34.381
−6.653
1.00
38.89
C


ATOM
16473
O
LEU
B
494
47.708
33.920
−5.513
1.00
39.50
O


ATOM
16475
N
PHE
B
495
48.399
33.951
−7.653
1.00
36.02
N


ATOM
16476
CA
PHE
B
495
49.338
32.845
−7.472
1.00
33.50
C


ATOM
16478
CB
PHE
B
495
49.496
32.044
−8.765
1.00
33.01
C


ATOM
16481
CG
PHE
B
495
48.244
31.366
−9.213
1.00
30.25
C


ATOM
16482
CD1
PHE
B
495
47.968
30.070
−8.824
1.00
26.23
C


ATOM
16484
CE1
PHE
B
495
46.812
29.435
−9.244
1.00
29.38
C


ATOM
16486
CZ
PHE
B
495
45.911
30.099
−10.063
1.00
30.71
C


ATOM
16488
CE2
PHE
B
495
46.174
31.397
−10.458
1.00
31.82
C


ATOM
16490
CD2
PHE
B
495
47.339
32.025
−10.033
1.00
30.88
C


ATOM
16492
C
PHE
B
495
50.701
33.340
−7.020
1.00
31.74
C


ATOM
16493
O
PHE
B
495
51.091
34.465
−7.308
1.00
29.96
O


ATOM
16495
N
ALA
B
496
51.426
32.474
−6.319
1.00
31.59
N


ATOM
16496
CA
ALA
B
496
52.781
32.785
−5.884
1.00
31.49
C


ATOM
16498
CB
ALA
B
496
53.264
31.747
−4.882
1.00
31.51
C


ATOM
16502
C
ALA
B
496
53.719
32.855
−7.092
1.00
30.52
C


ATOM
16503
O
ALA
B
496
53.483
32.208
−8.115
1.00
29.70
O


ATOM
16505
N
LYS
B
497
54.779
33.649
−6.967
1.00
29.89
N


ATOM
16506
CA
LYS
B
497
55.707
33.861
−8.075
1.00
30.53
C


ATOM
16508
CB
LYS
B
497
56.777
34.902
−7.707
1.00
31.65
C


ATOM
16511
CG
LYS
B
497
56.223
36.336
−7.603
1.00
37.13
C


ATOM
16514
CD
LYS
B
497
57.329
37.397
−7.715
1.00
43.09
C


ATOM
16517
CE
LYS
B
497
56.742
38.806
−7.904
1.00
48.67
C


ATOM
16520
NZ
LYS
B
497
57.647
39.736
−8.665
1.00
50.69
N


ATOM
16524
C
LYS
B
497
56.348
32.566
−8.613
1.00
28.82
C


ATOM
16525
O
LYS
B
497
56.487
32.417
−9.834
1.00
28.95
O


ATOM
16527
N
PRO
B
498
56.713
31.619
−7.724
1.00
26.35
N


ATOM
16528
CA
PRO
B
498
57.282
30.362
−8.229
1.00
25.10
C


ATOM
16530
CB
PRO
B
498
57.481
29.525
−6.957
1.00
24.98
C


ATOM
16533
CG
PRO
B
498
57.611
30.517
−5.873
1.00
24.86
C


ATOM
16536
CD
PRO
B
498
56.685
31.641
−6.252
1.00
25.79
C


ATOM
16539
C
PRO
B
498
56.383
29.619
−9.219
1.00
23.70
C


ATOM
16540
O
PRO
B
498
56.887
29.017
−10.162
1.00
23.87
O


ATOM
16541
N
PHE
B
499
55.069
29.659
−9.006
1.00
22.74
N


ATOM
16542
CA
PHE
B
499
54.141
29.015
−9.928
1.00
21.67
C


ATOM
16544
CB
PHE
B
499
52.787
28.746
−9.271
1.00
21.16
C


ATOM
16547
CG
PHE
B
499
51.789
28.128
−10.209
1.00
20.10
C


ATOM
16548
CD1
PHE
B
499
51.976
26.845
−10.681
1.00
17.88
C


ATOM
16550
CE1
PHE
B
499
51.084
26.281
−11.570
1.00
18.25
C


ATOM
16552
CZ
PHE
B
499
49.992
27.002
−11.997
1.00
16.84
C


ATOM
16554
CE2
PHE
B
499
49.798
28.282
−11.536
1.00
18.24
C


ATOM
16556
CD2
PHE
B
499
50.698
28.844
−10.655
1.00
19.01
C


ATOM
16558
C
PHE
B
499
53.955
29.831
−11.215
1.00
21.85
C


ATOM
16559
O
PHE
B
499
53.889
29.266
−12.311
1.00
20.84
O


ATOM
16561
N
VAL
B
500
53.875
31.154
−11.080
1.00
22.14
N


ATOM
16562
CA
VAL
B
500
53.711
32.033
−12.244
1.00
20.94
C


ATOM
16564
CB
VAL
B
500
53.614
33.507
−11.833
1.00
21.12
C


ATOM
16566
CG1
VAL
B
500
53.488
34.401
−13.066
1.00
17.61
C


ATOM
16570
CG2
VAL
B
500
52.425
33.717
−10.887
1.00
21.31
C


ATOM
16574
C
VAL
B
500
54.869
31.843
−13.217
1.00
21.15
C


ATOM
16575
O
VAL
B
500
54.672
31.808
−14.429
1.00
22.48
O


ATOM
16577
N
GLU
B
501
56.074
31.689
−12.683
1.00
21.08
N


ATOM
16578
CA
GLU
B
501
57.246
31.446
−13.510
1.00
20.88
C


ATOM
16580
CB
GLU
B
501
58.513
31.652
−12.677
1.00
22.06
C


ATOM
16583
CG
GLU
B
501
59.818
31.655
−13.477
1.00
23.77
C


ATOM
16586
CD
GLU
B
501
60.053
32.939
−14.246
1.00
25.66
C


ATOM
16587
OE1
GLU
B
501
59.226
33.870
−14.167
1.00
25.29
O


ATOM
16588
OE2
GLU
B
501
61.089
33.019
−14.934
1.00
29.65
O


ATOM
16589
C
GLU
B
501
57.213
30.037
−14.118
1.00
19.93
C


ATOM
16590
O
GLU
B
501
57.621
29.832
−15.256
1.00
20.86
O


ATOM
16592
N
THR
B
502
56.725
29.063
−13.364
1.00
19.13
N


ATOM
16593
CA
THR
B
502
56.559
27.720
−13.904
1.00
18.29
C


ATOM
16595
CB
THR
B
502
55.943
26.768
−12.870
1.00
17.85
C


ATOM
16597
OG1
THR
B
502
56.822
26.654
−11.745
1.00
18.95
O


ATOM
16599
CG2
THR
B
502
55.708
25.395
−13.475
1.00
18.10
C


ATOM
16603
C
THR
B
502
55.657
27.788
−15.130
1.00
18.19
C


ATOM
16604
O
THR
B
502
56.004
27.285
−16.200
1.00
18.59
O


ATOM
16606
N
ALA
B
503
54.507
28.435
−14.961
1.00
17.01
N


ATOM
16607
CA
ALA
B
503
53.574
28.662
−16.051
1.00
16.79
C


ATOM
16609
CB
ALA
B
503
52.397
29.513
−15.572
1.00
16.20
C


ATOM
16613
C
ALA
B
503
54.269
29.315
−17.254
1.00
16.84
C


ATOM
16614
O
ALA
B
503
54.203
28.794
−18.362
1.00
17.02
O


ATOM
16616
N
ILE
B
504
54.955
30.434
−17.035
1.00
15.79
N


ATOM
16617
CA
ILE
B
504
55.655
31.104
−18.129
1.00
16.42
C


ATOM
16619
CB
ILE
B
504
56.375
32.388
−17.663
1.00
16.84
C


ATOM
16621
CG1
ILE
B
504
55.353
33.465
−17.279
1.00
20.25
C


ATOM
16624
CD1
ILE
B
504
55.903
34.533
−16.379
1.00
15.32
C


ATOM
16628
CG2
ILE
B
504
57.283
32.920
−18.757
1.00
15.95
C


ATOM
16632
C
ILE
B
504
56.669
30.172
−18.797
1.00
16.44
C


ATOM
16633
O
ILE
B
504
56.842
30.214
−20.019
1.00
15.28
O


ATOM
16635
N
ASN
B
505
57.324
29.319
−18.010
1.00
16.41
N


ATOM
16636
CA
ASN
B
505
58.317
28.396
−18.574
1.00
17.85
C


ATOM
16638
CB
ASN
B
505
59.014
27.562
−17.486
1.00
18.33
C


ATOM
16641
CG
ASN
B
505
60.048
28.353
−16.700
1.00
16.57
C


ATOM
16642
OD1
ASN
B
505
60.589
29.350
−17.171
1.00
18.65
O


ATOM
16643
ND2
ASN
B
505
60.333
27.898
−15.500
1.00
16.08
N


ATOM
16646
C
ASN
B
505
57.736
27.470
−19.638
1.00
17.74
C


ATOM
16647
O
ASN
B
505
58.438
27.104
−20.579
1.00
18.71
O


ATOM
16649
N
LEU
B
506
56.463
27.102
−19.496
1.00
17.83
N


ATOM
16650
CA
LEU
B
506
55.774
26.300
−20.517
1.00
18.37
C


ATOM
16652
CB
LEU
B
506
54.326
26.030
−20.125
1.00
19.29
C


ATOM
16655
CG
LEU
B
506
53.554
25.067
−21.033
1.00
20.58
C


ATOM
16657
CD1
LEU
B
506
53.607
23.668
−20.465
1.00
18.95
C


ATOM
16661
CD2
LEU
B
506
52.110
25.515
−21.179
1.00
17.98
C


ATOM
16665
C
LEU
B
506
55.796
26.998
−21.871
1.00
17.25
C


ATOM
16666
O
LEU
B
506
56.022
26.363
−22.884
1.00
17.95
O


ATOM
16668
N
ALA
B
507
55.565
28.304
−21.876
1.00
16.97
N


ATOM
16669
CA
ALA
B
507
55.693
29.099
−23.089
1.00
16.34
C


ATOM
16671
CB
ALA
B
507
55.272
30.538
−22.831
1.00
14.72
C


ATOM
16675
C
ALA
B
507
57.130
29.048
−23.595
1.00
17.17
C


ATOM
16676
O
ALA
B
507
57.373
28.848
−24.784
1.00
17.32
O


ATOM
16678
N
ARG
B
508
58.089
29.212
−22.692
1.00
17.41
N


ATOM
16679
CA
ARG
B
508
59.487
29.194
−23.091
1.00
17.12
C


ATOM
16681
CB
ARG
B
508
60.397
29.443
−21.902
1.00
17.38
C


ATOM
16684
CG
ARG
B
508
60.275
30.838
−21.354
1.00
21.02
C


ATOM
16687
CD
ARG
B
508
61.325
31.143
−20.323
1.00
18.85
C


ATOM
16690
NE
ARG
B
508
61.271
32.565
−19.993
1.00
21.55
N


ATOM
16692
CZ
ARG
B
508
60.993
33.082
−18.797
1.00
20.04
C


ATOM
16693
NH1
ARG
B
508
60.760
32.313
−17.734
1.00
16.21
N


ATOM
16696
NH2
ARG
B
508
60.967
34.401
−18.665
1.00
21.31
N


ATOM
16699
C
ARG
B
508
59.836
27.874
−23.740
1.00
17.83
C


ATOM
16700
O
ARG
B
508
60.387
27.850
−24.841
1.00
17.31
O


ATOM
16702
N
GLN
B
509
59.501
26.779
−23.062
1.00
19.12
N


ATOM
16703
CA
GLN
B
509
59.777
25.440
−23.578
1.00
20.34
C


ATOM
16705
CB
GLN
B
509
59.297
24.377
−22.590
1.00
21.03
C


ATOM
16708
CG
GLN
B
509
59.548
22.947
−23.033
1.00
21.80
C


ATOM
16711
CD
GLN
B
509
60.998
22.676
−23.344
1.00
23.32
C


ATOM
16712
OE1
GLN
B
509
61.875
22.927
−22.528
1.00
27.09
O


ATOM
16713
NE2
GLN
B
509
61.261
22.158
−24.534
1.00
31.09
N


ATOM
16716
C
GLN
B
509
59.109
25.235
−24.941
1.00
22.09
C


ATOM
16717
O
GLN
B
509
59.669
24.563
−25.819
1.00
22.75
O


ATOM
16719
N
SER
B
510
57.922
25.824
−25.114
1.00
21.19
N


ATOM
16720
CA
SER
B
510
57.225
25.782
−26.392
1.00
20.82
C


ATOM
16722
CB
SER
B
510
55.862
26.469
−26.289
1.00
21.05
C


ATOM
16725
OG
SER
B
510
55.029
25.799
−25.358
1.00
19.96
O


ATOM
16727
C
SER
B
510
58.056
26.428
−27.501
1.00
20.73
C


ATOM
16728
O
SER
B
510
58.278
25.816
−28.550
1.00
20.43
O


ATOM
16730
N
HIS
B
511
58.528
27.649
−27.265
1.00
20.87
N


ATOM
16731
CA
HIS
B
511
59.375
28.343
−28.243
1.00
21.91
C


ATOM
16733
CB
HIS
B
511
59.892
29.675
−27.704
1.00
21.61
C


ATOM
16736
CG
HIS
B
511
58.862
30.754
−27.673
1.00
22.41
C


ATOM
16737
ND1
HIS
B
511
58.474
31.439
−28.803
1.00
22.39
N


ATOM
16739
CE1
HIS
B
511
57.558
32.333
−28.479
1.00
22.43
C


ATOM
16741
NE2
HIS
B
511
57.343
32.255
−27.178
1.00
25.82
N


ATOM
16743
CD2
HIS
B
511
58.147
31.275
−26.649
1.00
23.81
C


ATOM
16745
C
HIS
B
511
60.566
27.488
−28.630
1.00
22.41
C


ATOM
16746
O
HIS
B
511
60.896
27.380
−29.802
1.00
22.66
O


ATOM
16748
N
CYS
B
512
61.198
26.880
−27.632
1.00
23.19
N


ATOM
16749
CA
CYS
B
512
62.396
26.079
−27.850
1.00
24.69
C


ATOM
16751
CB
CYS
B
512
63.135
25.891
−26.530
1.00
24.68
C


ATOM
16754
SG
CYS
B
512
63.677
27.455
−25.811
1.00
27.11
S


ATOM
16756
C
CYS
B
512
62.121
24.715
−28.490
1.00
25.89
C


ATOM
16757
O
CYS
B
512
62.927
24.241
−29.280
1.00
25.89
O


ATOM
16759
N
THR
B
513
61.000
24.087
−28.140
1.00
27.54
N


ATOM
16760
CA
THR
B
513
60.627
22.795
−28.718
1.00
28.97
C


ATOM
16762
CB
THR
B
513
59.455
22.148
−27.962
1.00
28.82
C


ATOM
16764
OG1
THR
B
513
59.949
21.546
−26.763
1.00
29.92
O


ATOM
16766
CG2
THR
B
513
58.780
21.074
−28.805
1.00
26.03
C


ATOM
16770
C
THR
B
513
60.255
22.903
−30.193
1.00
31.24
C


ATOM
16771
O
THR
B
513
60.662
22.065
−30.996
1.00
30.43
O


ATOM
16773
N
TYR
B
514
59.496
23.939
−30.544
1.00
34.06
N


ATOM
16774
CA
TYR
B
514
58.957
24.083
−31.900
1.00
35.61
C


ATOM
16776
CB
TYR
B
514
57.484
24.473
−31.825
1.00
34.62
C


ATOM
16779
CG
TYR
B
514
56.666
23.420
−31.122
1.00
30.11
C


ATOM
16780
CD1
TYR
B
514
56.364
22.220
−31.750
1.00
30.04
C


ATOM
16782
CE1
TYR
B
514
55.617
21.237
−31.110
1.00
29.71
C


ATOM
16784
CZ
TYR
B
514
55.170
21.447
−29.824
1.00
31.94
C


ATOM
16785
OH
TYR
B
514
54.433
20.472
−29.193
1.00
34.49
O


ATOM
16787
CE2
TYR
B
514
55.461
22.634
−29.169
1.00
32.21
C


ATOM
16789
CD2
TYR
B
514
56.211
23.610
−29.821
1.00
30.80
C


ATOM
16791
C
TYR
B
514
59.770
25.053
−32.763
1.00
38.62
C


ATOM
16792
O
TYR
B
514
59.471
26.241
−32.859
1.00
38.41
O


ATOM
16794
N
HIS
B
515
60.796
24.502
−33.400
1.00
43.15
N


ATOM
16795
CA
HIS
B
515
61.773
25.263
−34.181
1.00
46.70
C


ATOM
16797
CB
HIS
B
515
63.159
25.123
−33.535
1.00
46.77
C


ATOM
16800
CG
HIS
B
515
63.598
23.700
−33.358
1.00
49.82
C


ATOM
16801
ND1
HIS
B
515
63.411
23.005
−32.181
1.00
50.36
N


ATOM
16803
CE1
HIS
B
515
63.884
21.779
−32.316
1.00
52.07
C


ATOM
16805
NE2
HIS
B
515
64.366
21.650
−33.539
1.00
52.64
N


ATOM
16807
CD2
HIS
B
515
64.196
22.835
−34.213
1.00
52.54
C


ATOM
16809
C
HIS
B
515
61.802
24.734
−35.622
1.00
48.71
C


ATOM
16810
O
HIS
B
515
60.968
23.898
−35.998
1.00
48.30
O


ATOM
16812
N
ASN
B
516
62.751
25.236
−36.417
1.00
51.12
N


ATOM
16813
CA
ASN
B
516
63.000
24.745
−37.779
1.00
53.12
C


ATOM
16815
CB
ASN
B
516
62.211
25.576
−38.807
1.00
53.90
C


ATOM
16818
CG
ASN
B
516
60.700
25.320
−38.754
1.00
56.25
C


ATOM
16819
OD1
ASN
B
516
60.234
24.209
−39.024
1.00
58.97
O


ATOM
16820
ND2
ASN
B
516
59.932
26.356
−38.417
1.00
56.55
N


ATOM
16823
C
ASN
B
516
64.494
24.791
−38.125
1.00
53.78
C


ATOM
16824
O
ASN
B
516
65.343
24.290
−37.379
1.00
54.33
O


ATOM
16826
N
HIS
B
520
74.438
24.782
−40.199
1.00
69.52
N


ATOM
16827
CA
HIS
B
520
73.028
24.665
−39.835
1.00
69.72
C


ATOM
16829
CB
HIS
B
520
72.146
25.329
−40.904
1.00
70.13
C


ATOM
16832
CG
HIS
B
520
71.042
26.175
−40.343
1.00
72.46
C


ATOM
16833
ND1
HIS
B
520
70.208
25.744
−39.332
1.00
75.25
N


ATOM
16835
CE1
HIS
B
520
69.335
26.694
−39.046
1.00
74.23
C


ATOM
16837
NE2
HIS
B
520
69.569
27.724
−39.839
1.00
74.51
N


ATOM
16839
CD2
HIS
B
520
70.629
27.425
−40.661
1.00
72.94
C


ATOM
16841
C
HIS
B
520
72.646
23.188
−39.656
1.00
69.01
C


ATOM
16842
O
HIS
B
520
73.382
22.295
−40.084
1.00
68.65
O


ATOM
16844
N
THR
B
521
71.502
22.942
−39.018
1.00
68.37
N


ATOM
16845
CA
THR
B
521
71.023
21.583
−38.746
1.00
68.19
C


ATOM
16847
CB
THR
B
521
71.182
21.227
−37.248
1.00
68.16
C


ATOM
16849
OG1
THR
B
521
72.440
21.714
−36.763
1.00
68.25
O


ATOM
16851
CG2
THR
B
521
71.108
19.719
−37.034
1.00
67.64
C


ATOM
16855
C
THR
B
521
69.550
21.433
−39.131
1.00
68.06
C


ATOM
16856
O
THR
B
521
68.817
22.422
−39.202
1.00
67.99
O


ATOM
16858
N
SER
B
522
69.128
20.194
−39.380
1.00
68.09
N


ATOM
16859
CA
SER
B
522
67.731
19.886
−39.699
1.00
68.28
C


ATOM
16861
CB
SER
B
522
67.643
18.538
−40.420
1.00
68.20
C


ATOM
16864
OG
SER
B
522
68.133
17.489
−39.602
1.00
67.15
O


ATOM
16866
C
SER
B
522
66.870
19.857
−38.424
1.00
68.80
C


ATOM
16867
O
SER
B
522
67.408
19.708
−37.325
1.00
68.89
O


ATOM
16869
N
PRO
B
523
65.532
19.999
−38.567
1.00
69.27
N


ATOM
16870
CA
PRO
B
523
64.611
19.985
−37.410
1.00
69.42
C


ATOM
16872
CB
PRO
B
523
63.221
20.001
−38.067
1.00
69.45
C


ATOM
16875
CG
PRO
B
523
63.430
20.682
−39.371
1.00
69.63
C


ATOM
16878
CD
PRO
B
523
64.820
20.313
−39.824
1.00
69.25
C


ATOM
16881
C
PRO
B
523
64.752
18.776
−36.458
1.00
69.17
C


ATOM
16882
O
PRO
B
523
64.897
18.969
−35.245
1.00
68.88
O


ATOM
16883
N
ASP
B
524
64.698
17.556
−37.002
1.00
68.83
N


ATOM
16884
CA
ASP
B
524
64.872
16.324
−36.208
1.00
68.18
C


ATOM
16886
CB
ASP
B
524
63.995
15.184
−36.749
1.00
68.01
C


ATOM
16889
CG
ASP
B
524
62.530
15.349
−36.388
1.00
69.08
C


ATOM
16890
OD1
ASP
B
524
62.227
15.878
−35.293
1.00
68.36
O


ATOM
16891
OD2
ASP
B
524
61.680
14.934
−37.202
1.00
69.43
O


ATOM
16892
C
ASP
B
524
66.341
15.896
−36.175
1.00
66.99
C


ATOM
16893
O
ASP
B
524
66.754
14.981
−36.891
1.00
67.26
O


ATOM
16895
N
GLU
B
525
67.097
16.568
−35.309
1.00
65.16
N


ATOM
16896
CA
GLU
B
525
68.556
16.430
−35.155
1.00
64.15
C


ATOM
16898
CB
GLU
B
525
69.310
16.361
−36.495
1.00
64.53
C


ATOM
16901
CG
GLU
B
525
69.815
14.955
−36.862
1.00
65.79
C


ATOM
16904
CD
GLU
B
525
70.828
14.966
−37.994
1.00
68.11
C


ATOM
16905
OE1
GLU
B
525
71.916
15.553
−37.813
1.00
68.91
O


ATOM
16906
OE2
GLU
B
525
70.540
14.377
−39.060
1.00
68.89
O


ATOM
16907
C
GLU
B
525
69.086
17.596
−34.313
1.00
62.63
C


ATOM
16908
O
GLU
B
525
70.042
17.434
−33.553
1.00
62.41
O


ATOM
16910
N
LEU
B
526
68.476
18.772
−34.474
1.00
60.84
N


ATOM
16911
CA
LEU
B
526
68.624
19.861
−33.503
1.00
59.29
C


ATOM
16913
CB
LEU
B
526
68.054
21.175
−34.051
1.00
59.08
C


ATOM
16916
CG
LEU
B
526
68.377
22.446
−33.255
1.00
58.48
C


ATOM
16918
CD1
LEU
B
526
69.872
22.756
−33.298
1.00
57.97
C


ATOM
16922
CD2
LEU
B
526
67.567
23.628
−33.773
1.00
56.17
C


ATOM
16926
C
LEU
B
526
67.921
19.479
−32.194
1.00
57.75
C


ATOM
16927
O
LEU
B
526
68.363
19.864
−31.111
1.00
57.36
O


ATOM
16929
N
THR
B
527
66.826
18.727
−32.307
1.00
56.15
N


ATOM
16930
CA
THR
B
527
66.132
18.165
−31.148
1.00
55.07
C


ATOM
16932
CB
THR
B
527
64.801
17.495
−31.562
1.00
54.60
C


ATOM
16934
OG1
THR
B
527
63.918
18.487
−32.102
1.00
54.27
O


ATOM
16936
CG2
THR
B
527
64.128
16.810
−30.373
1.00
51.53
C


ATOM
16940
C
THR
B
527
67.012
17.148
−30.420
1.00
55.32
C


ATOM
16941
O
THR
B
527
67.201
17.246
−29.205
1.00
55.42
O


ATOM
16943
N
ARG
B
528
67.551
16.183
−31.164
1.00
55.16
N


ATOM
16944
CA
ARG
B
528
68.395
15.140
−30.574
1.00
55.45
C


ATOM
16946
CB
ARG
B
528
68.781
14.084
−31.622
1.00
56.04
C


ATOM
16949
CG
ARG
B
528
69.600
12.906
−31.070
1.00
59.35
C


ATOM
16952
CD
ARG
B
528
69.957
11.888
−32.155
1.00
64.75
C


ATOM
16955
NE
ARG
B
528
70.689
12.485
−33.278
1.00
69.31
N


ATOM
16957
CZ
ARG
B
528
71.986
12.804
−33.273
1.00
72.03
C


ATOM
16958
NH1
ARG
B
528
72.741
12.600
−32.195
1.00
71.44
N


ATOM
16961
NH2
ARG
B
528
72.536
13.342
−34.361
1.00
72.52
N


ATOM
16964
C
ARG
B
528
69.649
15.726
−29.911
1.00
54.78
C


ATOM
16965
O
ARG
B
528
70.075
15.238
−28.863
1.00
55.19
O


ATOM
16967
N
LYS
B
529
70.230
16.765
−30.513
1.00
53.55
N


ATOM
16968
CA
LYS
B
529
71.410
17.426
−29.936
1.00
52.74
C


ATOM
16970
CB
LYS
B
529
72.099
18.339
−30.961
1.00
52.37
C


ATOM
16973
CG
LYS
B
529
72.943
17.560
−31.967
1.00
54.13
C


ATOM
16976
CD
LYS
B
529
73.914
18.429
−32.750
1.00
54.48
C


ATOM
16979
CE
LYS
B
529
74.734
17.567
−33.708
1.00
55.21
C


ATOM
16982
NZ
LYS
B
529
75.867
18.301
−34.338
1.00
55.62
N


ATOM
16986
C
LYS
B
529
71.061
18.201
−28.664
1.00
51.97
C


ATOM
16987
O
LYS
B
529
71.798
18.142
−27.679
1.00
51.98
O


ATOM
16989
N
ARG
B
530
69.934
18.911
−28.687
1.00
51.17
N


ATOM
16990
CA
ARG
B
530
69.453
19.645
−27.513
1.00
50.47
C


ATOM
16992
CB
ARG
B
530
68.253
20.534
−27.874
1.00
50.29
C


ATOM
16995
CG
ARG
B
530
68.628
21.833
−28.587
1.00
49.82
C


ATOM
16998
CD
ARG
B
530
67.393
22.633
−29.003
1.00
48.99
C


ATOM
17001
NE
ARG
B
530
67.732
23.924
−29.607
1.00
47.15
N


ATOM
17003
CZ
ARG
B
530
66.839
24.803
−30.067
1.00
49.12
C


ATOM
17004
NH1
ARG
B
530
65.537
24.547
−30.002
1.00
50.15
N


ATOM
17007
NH2
ARG
B
530
67.247
25.950
−30.595
1.00
50.51
N


ATOM
17010
C
ARG
B
530
69.088
18.694
−26.366
1.00
50.04
C


ATOM
17011
O
ARG
B
530
69.489
18.921
−25.217
1.00
49.90
O


ATOM
17013
N
VAL
B
531
68.342
17.633
−26.684
1.00
49.06
N


ATOM
17014
CA
VAL
B
531
67.954
16.627
−25.687
1.00
48.01
C


ATOM
17016
CB
VAL
B
531
67.043
15.520
−26.288
1.00
48.13
C


ATOM
17018
CG1
VAL
B
531
66.850
14.371
−25.295
1.00
46.58
C


ATOM
17022
CG2
VAL
B
531
65.695
16.096
−26.698
1.00
47.13
C


ATOM
17026
C
VAL
B
531
69.193
15.985
−25.066
1.00
46.96
C


ATOM
17027
O
VAL
B
531
69.256
15.795
−23.850
1.00
46.96
O


ATOM
17029
N
LEU
B
532
70.176
15.666
−25.906
1.00
45.51
N


ATOM
17030
CA
LEU
B
532
71.425
15.063
−25.439
1.00
44.29
C


ATOM
17032
CB
LEU
B
532
72.322
14.660
−26.624
1.00
44.69
C


ATOM
17035
CG
LEU
B
532
72.216
13.227
−27.179
1.00
45.27
C


ATOM
17037
CD1
LEU
B
532
70.780
12.702
−27.262
1.00
45.14
C


ATOM
17041
CD2
LEU
B
532
72.889
13.161
−28.540
1.00
44.64
C


ATOM
17045
C
LEU
B
532
72.185
15.994
−24.488
1.00
42.95
C


ATOM
17046
O
LEU
B
532
72.652
15.562
−23.432
1.00
42.21
O


ATOM
17048
N
SER
B
533
72.288
17.270
−24.860
1.00
41.47
N


ATOM
17049
CA
SER
B
533
73.056
18.252
−24.087
1.00
40.59
C


ATOM
17051
CB
SER
B
533
73.207
19.550
−24.884
1.00
40.46
C


ATOM
17054
OG
SER
B
533
71.951
20.188
−25.072
1.00
42.07
O


ATOM
17056
C
SER
B
533
72.425
18.559
−22.725
1.00
39.43
C


ATOM
17057
O
SER
B
533
73.117
18.985
−21.792
1.00
38.30
O


ATOM
17059
N
VAL
B
534
71.114
18.341
−22.628
1.00
38.04
N


ATOM
17060
CA
VAL
B
534
70.351
18.636
−21.420
1.00
37.19
C


ATOM
17062
CB
VAL
B
534
68.938
19.181
−21.773
1.00
36.30
C


ATOM
17064
CG1
VAL
B
534
67.966
18.984
−20.620
1.00
31.83
C


ATOM
17068
CG2
VAL
B
534
69.022
20.648
−22.172
1.00
32.17
C


ATOM
17072
C
VAL
B
534
70.226
17.429
−20.488
1.00
38.15
C


ATOM
17073
O
VAL
B
534
70.283
17.589
−19.269
1.00
38.33
O


ATOM
17075
N
ILE
B
535
70.059
16.233
−21.053
1.00
39.60
N


ATOM
17076
CA
ILE
B
535
69.784
15.035
−20.249
1.00
40.91
C


ATOM
17078
CB
ILE
B
535
68.569
14.222
−20.787
1.00
41.23
C


ATOM
17080
CG1
ILE
B
535
67.311
15.090
−20.903
1.00
40.90
C


ATOM
17083
CD1
ILE
B
535
66.884
15.730
−19.603
1.00
41.66
C


ATOM
17087
CG2
ILE
B
535
68.288
13.030
−19.874
1.00
41.53
C


ATOM
17091
C
ILE
B
535
70.969
14.084
−20.179
1.00
41.86
C


ATOM
17092
O
ILE
B
535
71.482
13.800
−19.094
1.00
42.32
O


ATOM
17094
N
THR
B
536
71.397
13.585
−21.334
1.00
42.66
N


ATOM
17095
CA
THR
B
536
72.316
12.446
−21.373
1.00
43.85
C


ATOM
17097
CB
THR
B
536
72.004
11.531
−22.584
1.00
44.49
C


ATOM
17099
OG1
THR
B
536
71.755
12.333
−23.745
1.00
47.98
O


ATOM
17101
CG2
THR
B
536
70.764
10.671
−22.301
1.00
44.85
C


ATOM
17105
C
THR
B
536
73.810
12.821
−21.340
1.00
43.27
C


ATOM
17106
O
THR
B
536
74.572
12.217
−20.588
1.00
43.08
O


ATOM
17108
N
GLU
B
537
74.220
13.811
−22.134
1.00
42.95
N


ATOM
17109
CA
GLU
B
537
75.645
14.184
−22.253
1.00
42.39
C


ATOM
17111
CB
GLU
B
537
75.965
14.643
−23.678
1.00
42.99
C


ATOM
17114
CG
GLU
B
537
76.089
13.519
−24.691
1.00
46.08
C


ATOM
17117
CD
GLU
B
537
76.491
14.026
−26.068
1.00
51.01
C


ATOM
17118
OE1
GLU
B
537
75.987
15.098
−26.479
1.00
52.31
O


ATOM
17119
OE2
GLU
B
537
77.309
13.354
−26.738
1.00
51.28
O


ATOM
17120
C
GLU
B
537
76.064
15.297
−21.281
1.00
40.65
C


ATOM
17121
O
GLU
B
537
75.546
16.414
−21.364
1.00
40.26
O


ATOM
17123
N
PRO
B
538
77.019
15.007
−20.377
1.00
38.40
N


ATOM
17124
CA
PRO
B
538
77.535
16.062
−19.502
1.00
36.97
C


ATOM
17126
CB
PRO
B
538
78.424
15.311
−18.493
1.00
36.84
C


ATOM
17129
CG
PRO
B
538
78.198
13.862
−18.723
1.00
38.72
C


ATOM
17132
CD
PRO
B
538
77.652
13.705
−20.107
1.00
39.09
C


ATOM
17135
C
PRO
B
538
78.370
17.087
−20.251
1.00
35.22
C


ATOM
17136
O
PRO
B
538
78.875
16.802
−21.334
1.00
34.58
O


ATOM
17137
N
ILE
B
539
78.505
18.268
−19.656
1.00
34.24
N


ATOM
17138
CA
ILE
B
539
79.364
19.312
−20.186
1.00
33.91
C


ATOM
17140
CB
ILE
B
539
79.039
20.688
−19.559
1.00
33.35
C


ATOM
17142
CG1
ILE
B
539
77.627
21.119
−19.946
1.00
33.50
C


ATOM
17145
CD1
ILE
B
539
77.251
22.499
−19.461
1.00
33.21
C


ATOM
17149
CG2
ILE
B
539
80.022
21.745
−20.023
1.00
31.40
C


ATOM
17153
C
ILE
B
539
80.808
18.925
−19.895
1.00
35.11
C


ATOM
17154
O
ILE
B
539
81.137
18.532
−18.772
1.00
34.38
O


ATOM
17156
N
LEU
B
540
81.654
19.016
−20.920
1.00
36.65
N


ATOM
17157
CA
LEU
B
540
83.070
18.673
−20.803
1.00
38.13
C


ATOM
17159
CB
LEU
B
540
83.811
19.047
−22.093
1.00
38.67
C


ATOM
17162
CG
LEU
B
540
83.700
18.017
−23.225
1.00
42.34
C


ATOM
17164
CD1
LEU
B
540
83.619
18.686
−24.610
1.00
45.32
C


ATOM
17168
CD2
LEU
B
540
84.862
17.015
−23.154
1.00
40.46
C


ATOM
17172
C
LEU
B
540
83.703
19.383
−19.603
1.00
38.81
C


ATOM
17173
O
LEU
B
540
83.588
20.603
−19.482
1.00
38.96
O


ATOM
17175
N
PRO
B
541
84.378
18.626
−18.717
1.00
39.33
N


ATOM
17176
CA
PRO
B
541
84.871
19.216
−17.476
1.00
40.17
C


ATOM
17178
CB
PRO
B
541
85.641
18.066
−16.813
1.00
39.97
C


ATOM
17181
CG
PRO
B
541
85.952
17.130
−17.903
1.00
39.37
C


ATOM
17184
CD
PRO
B
541
84.811
17.226
−18.857
1.00
39.33
C


ATOM
17187
C
PRO
B
541
85.785
20.412
−17.694
1.00
41.36
C


ATOM
17188
O
PRO
B
541
86.351
20.586
−18.777
1.00
41.39
O


ATOM
17189
N
PHE
B
542
85.902
21.228
−16.654
1.00
42.74
N


ATOM
17190
CA
PHE
B
542
86.712
22.434
−16.686
1.00
43.92
C


ATOM
17192
CB
PHE
B
542
86.505
23.217
−15.388
1.00
43.15
C


ATOM
17195
CG
PHE
B
542
87.402
24.405
−15.246
1.00
40.84
C


ATOM
17196
CD1
PHE
B
542
87.347
25.444
−16.163
1.00
39.06
C


ATOM
17198
CE1
PHE
B
542
88.182
26.548
−16.034
1.00
39.49
C


ATOM
17200
CZ
PHE
B
542
89.076
26.619
−14.974
1.00
37.53
C


ATOM
17202
CE2
PHE
B
542
89.132
25.591
−14.052
1.00
36.94
C


ATOM
17204
CD2
PHE
B
542
88.301
24.491
−14.192
1.00
38.64
C


ATOM
17206
C
PHE
B
542
88.196
22.106
−16.885
1.00
46.63
C


ATOM
17207
O
PHE
B
542
88.735
21.202
−16.244
1.00
46.58
O


ATOM
17209
N
GLU
B
543
88.838
22.841
−17.790
1.00
49.74
N


ATOM
17210
CA
GLU
B
543
90.273
22.697
−18.056
1.00
52.67
C


ATOM
17212
CB
GLU
B
543
90.500
22.140
−19.467
1.00
53.00
C


ATOM
17215
CG
GLU
B
543
89.851
20.773
−19.716
1.00
54.45
C


ATOM
17218
CD
GLU
B
543
89.450
20.557
−21.170
1.00
56.08
C


ATOM
17219
OE1
GLU
B
543
88.675
21.377
−21.711
1.00
55.47
O


ATOM
17220
OE2
GLU
B
543
89.904
19.557
−21.767
1.00
56.19
O


ATOM
17221
C
GLU
B
543
90.958
24.062
−17.902
1.00
54.66
C


ATOM
17222
O
GLU
B
543
90.371
25.100
−18.228
1.00
55.40
O


ATOM
17224
N
ARG
B
544
92.196
24.052
−17.411
1.00
56.34
N


ATOM
17225
CA
ARG
B
544
92.930
25.282
−17.094
1.00
57.83
C


ATOM
17227
CB
ARG
B
544
93.526
25.175
−15.685
1.00
58.69
C


ATOM
17230
CG
ARG
B
544
92.489
24.914
−14.599
1.00
61.11
C


ATOM
17233
CD
ARG
B
544
93.098
24.984
−13.205
1.00
65.32
C


ATOM
17236
NE
ARG
B
544
92.069
25.106
−12.167
1.00
68.58
N


ATOM
17238
CZ
ARG
B
544
91.674
24.138
−11.337
1.00
70.53
C


ATOM
17239
NH1
ARG
B
544
92.219
22.922
−11.371
1.00
71.79
N


ATOM
17242
NH2
ARG
B
544
90.720
24.395
−10.447
1.00
70.07
N


ATOM
17245
C
ARG
B
544
94.033
25.586
−18.119
1.00
58.07
C


ATOM
17246
O
ARG
B
544
93.768
25.795
−19.305
1.00
58.07
O


ATOM
17248
OXT
ARG
B
544
95.223
25.640
−17.797
1.00
58.05
O


ATOM
17249
O2
EDO
C
1
77.661
−43.007
−16.443
1.00
43.00
O


ATOM
17251
C2
EDO
C
1
76.783
−42.969
−15.309
1.00
44.22
C


ATOM
17254
C1
EDO
C
1
77.576
−42.916
−14.007
1.00
41.25
C


ATOM
17257
O1
EDO
C
1
78.695
−42.041
−14.162
1.00
43.89
O


ATOM
17259
O2
EDO
D
1
50.330
−35.935
6.828
1.00
44.73
O


ATOM
17261
C2
EDO
D
1
49.060
−36.295
7.378
1.00
45.21
C


ATOM
17264
C1
EDO
D
1
49.137
−37.691
7.987
1.00
45.66
C


ATOM
17267
O1
EDO
D
1
50.152
−37.735
8.995
1.00
39.86
O


ATOM
17269
O2
EDO
E
1
56.102
−25.006
−6.675
1.00
35.20
O


ATOM
17271
C2
EDO
E
1
55.115
−24.022
−6.312
1.00
39.87
C


ATOM
17274
C1
EDO
E
1
55.627
−22.972
−5.321
1.00
36.36
C


ATOM
17277
O1
EDO
E
1
56.896
−23.345
−4.763
1.00
38.87
O


ATOM
17279
O2
EDO
F
1
61.382
26.747
−13.014
1.00
37.16
O


ATOM
17281
C2
EDO
F
1
60.319
26.976
−12.075
1.00
39.91
C


ATOM
17284
C1
EDO
F
1
60.295
28.440
−11.630
1.00
42.58
C


ATOM
17287
O1
EDO
F
1
60.843
28.589
−10.311
1.00
42.11
O


ATOM
17289
O2
EDO
G
1
36.330
8.565
−39.005
1.00
47.10
O


ATOM
17291
C2
EDO
G
1
35.245
7.630
−39.096
1.00
49.42
C


ATOM
17294
C1
EDO
G
1
34.960
6.978
−37.743
1.00
48.88
C


ATOM
17297
O1
EDO
G
1
34.932
7.961
−36.704
1.00
45.94
O


ATOM
17299
O2
EDO
H
1
41.479
22.293
−17.292
1.00
35.35
O


ATOM
17301
C2
EDO
H
1
42.167
22.883
−16.188
1.00
36.60
C


ATOM
17304
C1
EDO
H
1
41.528
22.474
−14.866
1.00
36.89
C


ATOM
17307
O1
EDO
H
1
40.146
22.180
−15.068
1.00
40.64
O


ATOM
17309
O2
EDO
I
1
63.131
34.770
−14.264
1.00
54.30
O


ATOM
17311
C2
EDO
I
1
62.720
34.806
−12.892
1.00
53.62
C


ATOM
17314
C1
EDO
I
1
63.896
35.136
−11.984
1.00
51.08
C


ATOM
17317
O1
EDO
I
1
63.368
35.673
−10.768
1.00
48.31
O


ATOM
17319
O2
EDO
J
1
59.151
−30.232
2.286
1.00
44.49
O


ATOM
17321
C2
EDO
J
1
58.706
−30.950
1.125
1.00
42.28
C


ATOM
17324
C1
EDO
J
1
58.774
−30.052
−0.105
1.00
40.11
C


ATOM
17327
O1
EDO
J
1
59.229
−28.751
0.291
1.00
36.58
O


ATOM
17329
O2
EDO
K
1
57.235
−48.102
−15.555
1.00
69.20
O


ATOM
17331
C2
EDO
K
1
56.504
−48.728
−14.489
1.00
68.39
C


ATOM
17334
C1
EDO
K
1
55.610
−47.707
−13.786
1.00
67.00
C


ATOM
17337
O1
EDO
K
1
56.359
−46.520
−13.502
1.00
63.83
O


ATOM
17339
O2
EDO
L
1
62.259
−27.697
−29.101
1.00
41.14
O


ATOM
17341
C2
EDO
L
1
61.689
−26.383
−29.134
1.00
47.27
C


ATOM
17344
C1
EDO
L
1
60.177
−26.441
−28.906
1.00
47.64
C


ATOM
17347
O1
EDO
L
1
59.752
−27.796
−28.701
1.00
50.95
O


ATOM
17349
O2
EDO
M
1
81.050
−42.461
−15.315
1.00
47.87
O


ATOM
17351
C2
EDO
M
1
81.860
−42.153
−16.466
1.00
48.16
C


ATOM
17354
C1
EDO
M
1
81.056
−41.499
−17.586
1.00
47.34
C


ATOM
17357
O1
EDO
M
1
79.693
−41.937
−17.575
1.00
48.08
O


ATOM
17359
O2
EDO
N
1
28.354
−3.375
4.402
1.00
51.07
O


ATOM
17361
C2
EDO
N
1
29.104
−3.578
5.608
1.00
54.76
C


ATOM
17364
C1
EDO
N
1
28.967
−5.027
6.081
1.00
56.35
C


ATOM
17367
O1
EDO
N
1
29.372
−5.939
5.050
1.00
53.02
O


ATOM
17369
O2
EDO
O
1
48.766
0.661
−13.390
1.00
45.65
O


ATOM
17371
C2
EDO
O
1
50.117
1.103
−13.583
1.00
52.86
C


ATOM
17374
C1
EDO
O
1
50.184
2.630
−13.577
1.00
54.50
C


ATOM
17377
O1
EDO
O
1
50.984
3.091
−12.475
1.00
57.04
O


ATOM
17379
O
HOH
W
1
60.687
−41.700
3.860
1.00
11.23
O


ATOM
17382
O
HOH
W
2
57.035
−34.744
−10.325
1.00
19.60
O


ATOM
17385
O
HOH
W
3
57.953
−36.264
5.870
1.00
10.26
O


ATOM
17388
O
HOH
W
4
70.638
29.975
−18.112
1.00
18.09
O


ATOM
17391
O
HOH
W
5
84.932
24.597
−26.740
1.00
11.78
O


ATOM
17394
O
HOH
W
6
68.858
−38.920
7.795
1.00
19.90
O


ATOM
17397
O
HOH
W
7
39.088
11.823
10.564
1.00
10.95
O


ATOM
17400
O
HOH
W
8
48.412
−9.808
11.114
1.00
24.66
O


ATOM
17403
O
HOH
W
9
61.169
7.818
−25.284
1.00
31.12
O


ATOM
17406
O
HOH
W
10
47.599
7.531
13.059
1.00
18.61
O


ATOM
17409
O
HOH
W
11
64.039
−29.833
−3.258
1.00
16.41
O


ATOM
17412
O
HOH
W
12
42.935
−38.107
10.309
1.00
22.38
O


ATOM
17415
O
HOH
W
13
32.087
6.522
−8.854
1.00
18.71
O


ATOM
17418
O
HOH
W
14
31.936
−16.886
−1.578
1.00
27.02
O


ATOM
17421
O
HOH
W
15
37.784
29.459
−21.498
1.00
33.19
O


ATOM
17424
O
HOH
W
16
53.861
−30.927
12.149
1.00
27.93
O


ATOM
17427
O
HOH
W
17
70.063
31.639
−14.308
1.00
12.62
O


ATOM
17430
O
HOH
W
18
84.074
36.299
−9.580
1.00
12.24
O


ATOM
17433
O
HOH
W
19
39.692
35.076
−6.963
1.00
22.90
O


ATOM
17436
O
HOH
W
20
41.191
−34.300
−1.248
1.00
22.27
O


ATOM
17439
O
HOH
W
21
43.143
32.516
−47.841
1.00
10.32
O


ATOM
17442
O
HOH
W
22
30.933
−18.194
10.415
1.00
23.53
O


ATOM
17445
O
HOH
W
23
51.413
16.562
−9.613
1.00
17.16
O


ATOM
17448
O
HOH
W
24
53.453
−31.542
−1.575
1.00
27.60
O


ATOM
17451
O
HOH
W
25
34.185
9.497
9.218
1.00
20.29
O


ATOM
17454
O
HOH
W
26
70.482
−44.878
−22.511
1.00
19.16
O


ATOM
17457
O
HOH
W
27
61.126
−25.351
−11.285
1.00
30.79
O


ATOM
17460
O
HOH
W
28
82.202
46.630
−18.365
1.00
19.68
O


ATOM
17463
O
HOH
W
29
42.016
9.773
11.682
1.00
5.49
O


ATOM
17466
O
HOH
W
30
52.552
9.222
12.917
1.00
20.96
O


ATOM
17469
O
HOH
W
31
53.315
−4.004
7.707
1.00
22.13
O


ATOM
17472
O
HOH
W
32
64.807
−47.148
18.948
1.00
28.71
O


ATOM
17475
O
HOH
W
33
87.987
23.967
−22.136
1.00
15.14
O


ATOM
17478
O
HOH
W
34
61.924
43.652
−5.371
1.00
24.37
O


ATOM
17481
O
HOH
W
35
49.569
−32.537
13.686
1.00
27.90
O


ATOM
17484
O
HOH
W
36
77.969
23.424
−22.801
1.00
17.03
O


ATOM
17487
O
HOH
W
37
80.807
47.407
−31.377
1.00
8.36
O


ATOM
17490
O
HOH
W
38
46.696
−14.204
10.301
1.00
20.38
O


ATOM
17493
O
HOH
W
39
32.536
−20.230
2.767
1.00
30.33
O


ATOM
17496
O
HOH
W
40
67.794
−37.856
19.719
1.00
25.28
O


ATOM
17499
O
HOH
W
41
47.941
20.855
−36.616
1.00
23.14
O


ATOM
17502
O
HOH
W
42
62.977
54.761
−32.676
1.00
18.97
O


ATOM
17505
O
HOH
W
43
52.751
−50.583
−4.033
1.00
22.75
O


ATOM
17508
O
HOH
W
44
31.039
32.019
−32.115
1.00
31.94
O


ATOM
17511
O
HOH
W
45
42.029
26.557
1.145
1.00
29.11
O


ATOM
17514
O
HOH
W
46
31.869
4.285
−20.561
1.00
28.89
O


ATOM
17517
O
HOH
W
47
74.093
−42.929
−1.070
1.00
16.27
O


ATOM
17520
O
HOH
W
48
40.858
−34.802
−4.714
1.00
15.94
O


ATOM
17523
O
HOH
W
49
37.278
−5.018
−27.726
1.00
12.76
O


ATOM
17526
O
HOH
W
50
83.275
−36.108
−3.577
1.00
8.23
O


ATOM
17529
O
HOH
W
51
80.764
20.265
−23.409
1.00
16.65
O


ATOM
17532
O
HOH
W
52
31.595
3.922
2.971
1.00
23.66
O


ATOM
17535
O
HOH
W
53
57.415
−26.762
−3.471
1.00
28.77
O


ATOM
17538
O
HOH
W
54
72.526
10.018
−12.123
1.00
27.04
O


ATOM
17541
O
HOH
W
55
87.476
49.890
0.772
1.00
2.00
O


ATOM
17544
O
HOH
W
56
33.973
−23.668
7.434
1.00
24.56
O


ATOM
17547
O
HOH
W
57
52.409
−44.924
−11.036
1.00
25.63
O


ATOM
17550
O
HOH
W
58
51.617
−24.152
16.093
1.00
29.65
O


ATOM
17553
O
HOH
W
59
60.767
−28.228
−12.288
1.00
29.39
O


ATOM
17556
O
HOH
W
60
53.918
−56.125
0.008
1.00
28.78
O


ATOM
17559
O
HOH
W
61
33.210
−6.327
−3.576
1.00
34.39
O


ATOM
17562
O
HOH
W
62
41.640
14.903
3.307
1.00
15.68
O


ATOM
17565
O
HOH
W
63
58.660
−57.902
16.689
1.00
12.91
O


ATOM
17568
O
HOH
W
64
51.057
−36.200
−2.118
1.00
26.86
O


ATOM
17571
O
HOH
W
65
66.814
−24.153
22.063
1.00
35.44
O


ATOM
17574
O
HOH
W
66
29.022
−28.733
26.291
1.00
17.80
O


ATOM
17577
O
HOH
W
67
90.189
−22.222
4.963
1.00
19.58
O


ATOM
17580
O
HOH
W
68
78.682
−42.748
19.875
1.00
28.90
O


ATOM
17583
O
HOH
W
69
56.469
−31.804
−2.878
1.00
31.63
O


ATOM
17586
O
HOH
W
70
41.401
20.399
−1.163
1.00
29.63
O


ATOM
17589
O
HOH
W
71
53.598
−11.238
8.837
1.00
28.06
O


ATOM
17592
O
HOH
W
72
75.612
−15.475
−6.124
1.00
23.91
O


ATOM
17595
O
HOH
W
73
68.376
−34.458
−18.543
1.00
16.52
O


ATOM
17598
O
HOH
W
74
69.339
−31.552
−15.520
1.00
17.52
O


ATOM
17601
O
HOH
W
75
69.286
−41.965
−19.351
1.00
24.29
O


ATOM
17604
O
HOH
W
76
75.463
−46.593
−7.759
1.00
19.91
O


ATOM
17607
O
HOH
W
77
63.711
−35.486
−0.007
1.00
5.12
O


ATOM
17610
O
HOH
W
78
62.685
−33.036
0.733
1.00
15.71
O


ATOM
17613
O
HOH
W
79
65.190
−28.298
0.653
1.00
17.26
O


ATOM
17616
O
HOH
W
80
74.263
−38.930
−2.404
1.00
12.17
O


ATOM
17619
O
HOH
W
81
76.242
−38.951
−0.599
1.00
14.40
O


ATOM
17622
O
HOH
W
82
65.958
−51.251
6.374
1.00
21.04
O


ATOM
17625
O
HOH
W
83
64.794
−53.762
7.147
1.00
16.67
O


ATOM
17628
O
HOH
W
84
69.920
−48.364
6.636
1.00
23.24
O


ATOM
17631
O
HOH
W
85
59.007
−35.681
0.128
1.00
21.30
O


ATOM
17634
O
HOH
W
86
47.141
−47.469
0.295
1.00
8.84
O


ATOM
17637
O
HOH
W
87
32.623
28.895
−35.533
1.00
4.99
O


ATOM
17640
O
HOH
W
88
49.914
−40.645
8.973
1.00
22.44
O


ATOM
17643
O
HOH
W
89
43.107
−38.127
1.800
1.00
14.68
O


ATOM
17646
O
HOH
W
90
42.398
−41.782
7.963
1.00
17.43
O


ATOM
17649
O
HOH
W
91
41.442
−48.239
5.460
1.00
2.00
O


ATOM
17652
O
HOH
W
92
62.411
−31.955
8.352
1.00
20.44
O


ATOM
17655
O
HOH
W
93
60.871
−20.177
4.347
1.00
20.57
O


ATOM
17658
O
HOH
W
94
58.610
−23.989
0.527
1.00
15.61
O


ATOM
17661
O
HOH
W
95
77.170
−36.427
−8.109
1.00
17.54
O


ATOM
17664
O
HOH
W
96
69.010
−23.558
−4.094
1.00
5.17
O


ATOM
17667
O
HOH
W
97
67.976
−19.417
−1.601
1.00
27.30
O


ATOM
17670
O
HOH
W
98
55.120
−15.695
−9.971
1.00
15.80
O


ATOM
17673
O
HOH
W
99
40.221
−8.919
14.184
1.00
24.31
O


ATOM
17676
O
HOH
W
100
75.311
−46.315
−17.890
1.00
12.75
O


ATOM
17679
O
HOH
W
101
36.689
12.147
−8.452
1.00
13.56
O


ATOM
17682
O
HOH
W
102
49.409
4.411
−3.957
1.00
20.53
O


ATOM
17685
O
HOH
W
103
51.208
−3.216
−6.796
1.00
27.29
O


ATOM
17688
O
HOH
W
104
50.423
−11.431
−14.044
1.00
25.17
O


ATOM
17691
O
HOH
W
105
35.050
−4.248
−9.227
1.00
15.36
O


ATOM
17694
O
HOH
W
106
40.522
−39.592
8.121
1.00
13.31
O


ATOM
17697
O
HOH
W
107
44.392
−41.412
−4.710
1.00
25.85
O


ATOM
17700
O
HOH
W
108
90.651
−25.293
0.822
1.00
20.51
O


ATOM
17703
O
HOH
W
109
75.686
25.603
−1.188
1.00
19.35
O


ATOM
17706
O
HOH
W
110
79.161
27.994
−0.849
1.00
24.23
O


ATOM
17709
O
HOH
W
111
30.975
6.748
−35.336
1.00
22.85
O


ATOM
17712
O
HOH
W
112
81.633
50.321
0.940
1.00
16.36
O


ATOM
17715
O
HOH
W
113
81.501
41.972
−14.894
1.00
5.20
O


ATOM
17718
O
HOH
W
114
77.799
33.970
−22.161
1.00
19.08
O


ATOM
17721
O
HOH
W
115
69.029
32.450
−3.346
1.00
27.97
O


ATOM
17724
O
HOH
W
116
67.333
54.975
−36.644
1.00
5.32
O


ATOM
17727
O
HOH
W
117
71.581
36.620
−26.421
1.00
26.94
O


ATOM
17730
O
HOH
W
118
68.657
33.764
−26.041
1.00
20.83
O


ATOM
17733
O
HOH
W
119
57.118
40.798
−25.352
1.00
22.78
O


ATOM
17736
O
HOH
W
120
57.109
43.195
−30.729
1.00
20.51
O


ATOM
17739
O
HOH
W
121
64.944
22.448
−22.022
1.00
23.64
O


ATOM
17742
O
HOH
W
122
65.055
24.670
−18.404
1.00
17.02
O


ATOM
17745
O
HOH
W
123
73.576
24.897
−13.394
1.00
9.07
O


ATOM
17748
O
HOH
W
124
50.432
18.994
−27.696
1.00
23.95
O


ATOM
17751
O
HOH
W
125
42.778
15.381
−31.371
1.00
23.30
O


ATOM
17754
O
HOH
W
126
43.937
16.439
−33.443
1.00
40.87
O


ATOM
17757
O
HOH
W
127
30.460
12.584
−23.332
1.00
27.55
O


ATOM
17760
O
HOH
W
128
31.399
25.056
−38.256
1.00
17.13
O


ATOM
17763
O
HOH
W
129
45.704
22.675
−34.062
1.00
34.00
O


ATOM
17766
O
HOH
W
130
35.949
19.963
−9.142
1.00
30.59
O


ATOM
17769
O
HOH
W
131
40.354
36.564
−35.731
1.00
19.72
O


ATOM
17772
O
HOH
W
132
38.318
34.494
−35.413
1.00
7.26
O


ATOM
17775
O
HOH
W
133
57.188
36.811
−31.845
1.00
24.17
O


ATOM
17778
O
HOH
W
134
58.146
38.433
−16.715
1.00
27.12
O


ATOM
17781
O
HOH
W
135
92.188
21.084
−22.452
1.00
21.22
O


ATOM
17784
O
HOH
W
136
50.818
−42.619
−4.261
1.00
23.46
O


ATOM
17787
O
HOH
W
137
41.418
−14.439
3.492
1.00
24.49
O


ATOM
17790
O
HOH
W
138
60.691
−22.131
0.873
1.00
26.59
O


ATOM
17793
O
HOH
W
139
63.812
−50.432
−16.555
1.00
18.97
O


ATOM
17796
O
HOH
W
140
66.799
−43.436
−15.556
1.00
26.39
O


ATOM
17799
O
HOH
W
141
36.831
23.369
−20.144
1.00
24.83
O


ATOM
17802
O
HOH
W
142
69.526
−39.429
2.654
1.00
17.65
O


ATOM
17805
O
HOH
W
143
76.413
42.942
0.826
1.00
32.70
O


ATOM
17808
O
HOH
W
144
43.360
−29.899
−8.145
1.00
33.98
O


ATOM
17811
O
HOH
W
145
49.576
35.961
−9.667
1.00
26.92
O


ATOM
17814
O
HOH
W
146
47.057
−32.984
−9.280
1.00
33.16
O


ATOM
17817
O
HOH
W
147
54.276
−39.999
−12.465
1.00
31.06
O


ATOM
17820
O
HOH
W
148
49.138
−11.535
−7.738
1.00
21.23
O


ATOM
17823
O
HOH
W
149
51.464
33.092
−16.231
1.00
39.45
O


ATOM
17826
O
HOH
W
150
37.234
23.654
−4.671
1.00
29.14
O


ATOM
17829
O
HOH
W
151
54.632
−49.110
−8.260
1.00
27.54
O


ATOM
17832
O
HOH
W
152
32.712
−14.896
2.375
1.00
24.37
O


ATOM
17835
O
HOH
W
153
40.313
31.980
−26.077
1.00
18.77
O


ATOM
17838
O
HOH
W
154
63.860
−40.924
4.353
1.00
27.96
O


ATOM
17841
O
HOH
W
155
47.606
−39.199
11.721
1.00
31.76
O


ATOM
17844
O
HOH
W
156
46.777
−7.365
16.313
1.00
32.28
O


ATOM
17847
O
HOH
W
157
39.606
−17.036
−19.091
1.00
24.83
O


ATOM
17850
O
HOH
W
158
45.372
−31.614
−12.501
1.00
30.91
O


ATOM
17853
O
HOH
W
159
59.419
−24.763
13.202
1.00
40.00
O


ATOM
17856
O
HOH
W
160
86.758
−24.726
6.593
1.00
15.69
O


ATOM
17859
O
HOH
W
161
78.705
45.595
5.075
1.00
26.92
O


ATOM
17862
O
HOH
W
162
75.448
26.421
−14.430
1.00
18.50
O


ATOM
17865
O
HOH
W
163
59.126
47.335
−13.817
1.00
25.69
O


ATOM
17868
O
HOH
W
164
57.117
51.918
−18.780
1.00
21.01
O


ATOM
17871
O
HOH
W
165
68.807
35.000
−18.130
1.00
18.00
O


ATOM
17874
O
HOH
W
166
65.330
37.902
−18.096
1.00
30.56
O


ATOM
17877
O
HOH
W
167
53.156
15.720
−3.604
1.00
22.56
O


ATOM
17880
O
HOH
W
168
58.196
19.642
−7.395
1.00
22.98
O


ATOM
17883
O
HOH
W
169
55.559
23.000
−41.753
1.00
16.76
O


ATOM
17886
O
HOH
W
170
64.909
32.691
−17.810
1.00
40.95
O

## Claims

1. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to K134, K138, L143, I156, E159, F163, S166, H167, E170, K414, or Q421 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of K134P, K138C, L143F, L143V, I156G, E159G, E159Q, F163C, F163E, F163Q, F163V, F163Y, S166C, S166D, S166G, S166P, S166V, H167M, E170G, E170H, E170K, E170N, E170R, E170S, E170W, K414F, K414G, K414N, K414P, and Q421R, and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 under similar growth conditions.

2. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to E29, N47, S86, K94, E131, K134, I156, V162, K169, K178, E179, S231, R242, F369, K414, or Q421 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of E29N, N47V, S86C, K94A, E131F, K134E, K134P, I156G, V162P, K169C, K178E, E179T, S231D, S231K, S231R, S231T, S231V, R242N, R242I, F369C, K414C, K414F, K414G, K414N, and Q421D, and wherein a host cell expressing the polypeptide has at least 50% more growth compared to the growth of a host cell expressing a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 under similar growth conditions.

3. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to K50, D81, K134, I137, L143, I156, E159, S166, H167, K169, E170, or K414 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of K50S, D81F, K134E, K134P, I137N, L143V, I156G, E159D, E159G, E159Q, S166C, S166W, H167M, H167N, K169C, E170H, E170K, E170W, K414C, K414F, K414G, K414N, and K414P, and wherein a host cell expressing the polypeptide has at least 30% more growth compared to the growth of a host cell expressing a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 under similar growth conditions.

4. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to V30, V84, K134, I140, L143, F163, S166, K169, E170 or S172 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of V30K, V84T, K134C, K134D, K134E, I140S, I140T, L143F, L143I, L143M, L143V, F163I, F163M, S166P, S166V, K169Q, E170H, E170K, and S172V, and wherein the polypeptide has at least 20% increase in specific activity of isoprene synthase compared to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO: 1 and at least 20% more growth in a host cell expressing the polypeptide compared to the growth of a host cell expressing a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO: 1 under similar growth conditions.

5. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide with isoprene synthase properties wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to N47, G87, I156, V162, E170, S231, R242, V409, or K414 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of N47V, G87R, I156G, V162P, E170H, S231T, R242N, V409T, and K414F, and wherein the polypeptide has at least 30% increase in specific activity of isoprene synthase as compared to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 and at least 30% more growth when the polypeptide is expressed in a host cell, as compared to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 under similar growth conditions.

6. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide with isoprene synthase properties wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to E2, Y18, L19, S21, T24, E26, S27, E29, K37, V42, N47, N48, E49, L56, D81, R82, V84, T93, K94, T95, S120, K123, N126, E131, N132, K134, I137, A139, L143, L151, N155, S166, H167, K169, E170, L171, K175, E179, L180, Q197, I229, S231, T240, R242, R245, R246, V247, T251, A271, S282, L306, D317, N319, F369, Q371, L376, K379, S380, G389, W392, K393, V408, V409, Q421, K422, Y423, R429, C437, S444, I447, S455, C458, R461, G464, S466, A470, S473, V500, T502, L506, T513, E525 or V531 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of E2A or K or P, Y18D or E or K or S, L19Y, S21W, T24L or V, E26C, S27D or N, E29N, K37C or D or Por Q or S, V42M, N47D or S, N48D or G or T, E49L or V, L56E or F or G or I or K or T or V or Y, D81Q, R82N or T or V or Y, V84M, T93C or F or R or S, K94G or P, T95D or F or G or I or N or W, S120C or G or M or Q, K123V, N126E, E131H or K or L or M or T or W or Y, N132I or P, K134A, I137T, A139C or Q, L143C or D or E or H or K or M or Q or T or V or Y, L151A or F, N155A or C or G or H or Q or R or S or W, S166N, H167F or I or N or Q or V, K169A or C or H or N or Q or V, E170L or S or W or Y, L171A or N or Q or T or V or Y, K175C or F or I or Q or R, E179D, L180A or I, Q197C or D or N, I229C, S231A, T240C, R242G, R245C or K or M or Q or T or V, R246N, V247L or M, T251D or E or N or P or Q or S, A271T, S282Y, L306C, D317N, N319M, F369C or D or E or G or S, Q371F, L376I or M, K379G or Q, S380E, G389A or D or E or K or N or Q or S or V, W392Y, K393C or I or T or V, V408T, V409T, Q421H, K422D, Y423N or S, R429E or F or Q, C437M, S444D or E, I447T, S455A, C458T, R461A, G464C or M or N or Q or S, S466D, A470I or L, S473I, V500A or C, T502M, L506M, T513C or G or K or N, E525F or R, and V531E or H or K or Q or R or S, and wherein the polypeptide has (i) minimum performance indices (PI) relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO: 1 for specific activity and expression are greater than or equal to a PI of 0.9 and where at least one PI relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.0; or (ii) minimum performance indices (PI) relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.8 and where at least one PI relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.2; or (iii) minimum performance indices (PI) relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.5 and where at least one PI relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.5.

7. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide with improved isoprene synthase properties wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to E2, S6, Y18, L20, S22, D23, T24, D25, E26, S27, I28, E29, V30, Y31, K32, K36, K37, V42, R44, N47, N48, E49, K50, F53, L54, T55, L56, E58, L59, L68, R71, S74, R77, G78, A79, D81, R82, F83, V84, S86, G87, A91, T93, K94, T95, L97, H98, G99, Q109, S115, Q116, E117, A118, S120, K123, Q125, N126, G127, N128, L130, E131, N132, L133, K134, D136, I137, K138, A139, I140, L143, L151, G153, N155, I156, E159, A160, K161, V162, F163, A164, S166, H167, K169, E170, L171, S172, K175, I176, G177, K178, E179, L180, A181, E182, L190, R194, Q197, S204, K211, N215, V217, L219, L221, M228, I229, S231, V232, R235, S241, R242, R245, R246, V247, T251, H254, A271, F272, D278, C279, S282, I296, T302, D317, N319, A320, Y327, C331, K348, G351, Y357, A361, D364, L365, A368, F369, L370, Q371, A373, Y377, S380, T383, D386, G389, W392, K393, A407, V408, V409, Q410, N411, K414, K422, Y423, H424, S428, R429, H432, L436, C437, L440, S444, I447, A448, S457, M460, R461, T462, K463, G464, I465, S466, E468, A470, T471, E472, S473, M475, E480, L490, G492, L494, A496, V500, E501, T502, A503, S510, T513, H515, A519, E525, V531, T536, E537, L540, P541, F542, or R544 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of E2C or D or N or T or V, S6N or T, Y18A or Q or R, L20T, S22Q, D23N, T24C, D25T, E26D or H or K or M or R or S or V, S27A or C or G or H or I or L or M or P or Q, 128D or N, E29Q, V30A or D or E or M or R or T, Y31N, K32E, K36A or C or D or E or M or N or P or Q, K37A or E or G or H or M or N or R or T, V42F or I, R44N or Q, N47A or G or H or M or Q or T or W, N48H or I or K, E49A or C, K50A or D or E or F or H or S or Y, F53E or H or N or P or Q or V, L54M, T55C or D or E, L56C or N, E58N, L59H or T, L68I, R71K or M, S74D or E or N or Y, R77L, G78A or D or F or L or M, A79Q or T, D81A or F or G or M or R or S or T or V, R82A or E or H or I or K or M or Q or S, F83W, V84A, S86A or D or M, G87D or P, A91K or W, T93A or D or E or G or L or N or P or Y, K94A or D or E or H or I or L or M or N or R or S or T, T95A or E or P or Q or S or V or Y, L97F, H98A or D or F or G or I or L or M or N or Q, G99E or F or M, Q109E, S115A, Q116A or C or D or E or I or P, E117C or F or L or M or V, A118M, S120H or T or V, K123L or T, Q125E or I or Y, N126A or C or D or M or T or V, G127C, N128C or D or P or Q, L130E, E131A or C or P or Q or S or V, N132C or D or F or H or L or R or W or Y, L133D, K134E or M or Q or S or T or V, D136E, I137E or H or N, K138I or N, A139N, I140M or W, L143S, L151C or H or I, G153C, N155I or T or V or Y, I156D or N or T, E159M, A160I, K161A or C or N or Q, V162S, F163E or Q, A164T, S166A or D or G, H167A or E or G or K or M or R or S or T or W, K169D or I or M or S or T, E170H or K or M or Q or T or V, L171H or K or R or S, S172A or C, K175S, I176M, G177A or C, K178A or F or R or S or T, E179A or C or L or M or N, L180C or Q or T, A181H or Q or S or V, E182S, L190I or M, R194L, Q197S, S204C, K211A or N or Q, N215C or H, V217I, L219C, L221M, M228F or Y, I229V, S231K or Q or T, V232I, R235K, S241A or M or T, R242A or D or E or H or I or M or N or Q or S or T, R245I or L, R246D or K, V247T, T251A or G or K or R, H254D, A271C or V, F272D or G or P or W, D278A or E or N or Q or S or T or V or W, C279A, S282A or Q, I296V, T302H, D317E or Q, N319F, A320C, Y327M, C331P, K348R or Y, G351D or N, Y357M, A361T, D364E or V, L365C or M, A368N, F369M or N or R or T or V, L370G or Q, Q371C or S, A373G, Y377W, S380A or C or D or Q or T or V, T383S, D386E or N, G389H or I, W392I or S or T or V, K393Q, A407G, V408I, V409H or I, Q410C or D or K or L or M or T, N411G, K414E or G or L or N or P, K422A or N or T, Y423Q, H424E or P or Q or V, S428E or Q, R429I or L or T or W or Y, H432E, L436M or Y, C437K or T, L440I, S444P, I447A or E or M or Q or S, A448E or M or N or P or Q or V, S457N or T, M460Q or R or S, R461D or E or G or Q or S or T, T462Q, K463A or D or E, G464L or R, I465A or C or G or S or T, S466P, E468D, A470M, T471E or H or Q, E472D or S, S473L or V, M475T, E480N, L490A or D or E or F or H or M, G492C, L494D, A496P or T, V500L or M, E501D, T502A or C or R or V, A503I, S510C or V, T513V, H515N, A519S or T, E525A or C or P or Q or S, V531A or M or T, T536A or F or G, E537K or T, L540A or P, P541M, F542P, and R544C, and wherein the polypeptide has (i) minimum performance indices (P1) relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.9 and where at least one PI relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.0; or (ii) minimum performance indices (P1) relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity and expression are greater than or equal to a PI of 0.8 and where at least one PI relative to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 for specific activity or growth is greater than or equal to a PI of 1.2.

8. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to S22, K36, R43, E58, G87, F89, A118, L151, G153, Q234, V247, H254, S282, A391, W392, C437, I447, T481, E488, T502 or F542 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of S22K or R, K36H or W, R43E, E58F, G87S or R, F89D, A118E, L151Y, G153P, Q234R, V247I, H254C, S282H or W, or T or Y, A391G, W392C, C437L, I447V, T481Y, E488L, T502F and F542N, and wherein the polypeptide has at least 30% increase in specific activity of isoprene synthase compared to a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1.

9. A recombinant microbial host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity and providing improved growth activity when the polypeptide is expressed in a host cell, wherein the polypeptide has at least 90% sequence identity to SEQ ID NO:1 and comprises a substitution at a residue corresponding to V30, K134, L143, I156, E159, S172, K414, or Q421 of SEQ ID NO:1, wherein the substitution is selected from the group consisting of V30K, K134C or P, L143I, I156G, E159D, S172V, K414F, and Q421R or D, and wherein a host cell expressing the polypeptide has at least 20% more growth compared to the growth of a host cell expressing a parent polypeptide which does not comprise said substitution at a residue corresponding to SEQ ID NO:1 under similar growth conditions.

10. The host cell of any one of claims 1-9 wherein the host cell is selected from the group consisting of a bacterial, algal, fungal, yeast, cyanobacterial, and Clostridial cell.

11. The host cell of claim 10 wherein the host cell is a bacterial cell.

12. The host cell of claim 11 wherein the bacterial cell is a gram-positive bacterial cell or gram-negative bacterial cell.

13. The host cell of claim 12 wherein the bacterial cell is selected from the group consisting of a E. coli, L. acidophilus, P. citrea, B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans, B. lautus, B. thuringiensis, S. albus, S. lividans, S. coelicolor, S. griseus, Pseudomonas sp., P. alcaligenes, Clostridium sp., Corynebacterium sp., and C. glutamicum cell.

14. The host cell of claim 10 wherein the host cell is an algal cell.

15. The host cell of claim 14 wherein the algal cell is selected from the group consisting of green alga, red alga, glaucophyte, chlorarachniophyte, euglenid, chromista, and dinoflagellate cell.

16. The host cell of claim 15 wherein the host cell is a fungal cell.

17. The host cell of claim 16 wherein the fungal cell is a filamentous fungi.

18. The host cell of claim 10 wherein the host cell is a yeast cell.

19. The host cell of claim 18 wherein the yeast cell is selected from the group consisting of a Saccharomyces sp., Schizosaccharomyces sp., Pichia sp., and Candida sp. cell.

20. The host cell of claim 19 wherein the yeast cell is a Saccharomyces cerevisiae cell.