# Bioassays of the City of



# Homestead Municipal Power Plant

Miami-Dade County

NPDES #FL0002721

Sampled February 13, 2006

April 2006

Biology Section

Bureau of Laboratories

Division of Resource Asses sment and Management

Quality Manual No. 870346G

NELAC Certification No. E31780

## 1 City of Homestead Municipal Power Plant, 675 N. Flagler Avenue, Homestead, Miami-Dade County, Florida, NPDES #FL0002721. Effluent samples for this facility were collected on February 13, 2006.



## Introduction
The City of Homestead Municipal Power Plant is an electric power generating plant having a peak electric power generating capacity of 53 megawatts. The facility has a design flow of 4.11 million gallons per day (MGD). The facility had an average annual daily flow (AADF) of 1.35 MGD from February 2005 through January 2006. The facility has 16 gen erating units, of which two units ar e cooled by an air cooling system, two units are cooled by a mechanical-draft cooling to wer system and 12 units ar e cooled by once-through cooling water supplied from on-site wells. Chemical additives are not added to this cooling water. This once-through cooling water is passed through an on-s ite double oil-water separator treatment system prior to discharge through Outfall D-001 to a pipe (approximately 624 m [2050 feet] long) which discharges into the Class III freshw aters of an unnamed drainage ditch t hat runs into the C-103S Canal, thence into Biscayne Bay. The origin of the outfall pi peline, or D-001, is located approximately at latitude 25o 28’ 27” N, longitude 80o 28’ 12” W. The point wher e the pipeline ends at the unnamed ditch is located approximately at latitude 25o 28’ 08” N, longitude 80o 28’ 11” W.

The facility is not currently under any Administrative Or ders (AO) or Consent Orders (CO). The facility has no toxicity testing requirements or mixing zones. Du ring the past year, the facility had no permit violations. (Facility information provided by Terry Davis of FDEP Sout heast District).



## Methods
Samples were collected from the EFF-01 NPDES samp ling point for Outfall D-001 by Terry Davis (FDEP Southeast District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOP’s are available on the web at: http://www.floridadep.org/labs/qa/sops.htm

.

See Appendix 1 for the completed chain of custody form.

The 48-hour acute screening toxicity tests and alga l growth potential test were performed following internal DEP SOP’s TA07_01, TA07_02, and TA08_01 through TA08_09. All internal DEP SOP’s are available on the web at: http://www.flor idadep.org/labs/cgi-bin/sop/biosop.asp . The Department uses the 48-hour acute screening toxicity test to provide re asonable assurance that the facility does not adversely

affect waters of the state.



## Toxicity Test Results
Tests were performed February 14 - 16, 2006.

Ceriodaphnia dubia 48-hour acute screening bioassay – LC 50 

## > 100%, 0% mortality* in 100% sample at

48 hours

Cyprinella leedsi 48-hour acute screening bioassay – LC 50 

## > 100%, 0% mortality* in 100% sample

at 48 hours

The bioassay sample was not acutely toxic to the test organisms Ceriodaphnia dubia and Cyprinella

leedsi

.

See Appendix 2 for bioassay bench sheets.

See Appendix 3 for results of the last 20 standard re ference toxicant (SRT) tests and bench sheets and statistical analyses of the SRT test that corresponds to this facility te st. See Appendix 4 for test organism receipts.

## 2 *NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides reasonable assurance that the effluent meets the acute toxicity criterion of Florida’s Surface Water Quality Standards (Rule 62-302.500 (1)(a)4, F.A.C.). Mo rtality between 20-50% mortality indicates low to moderate levels of toxicity, and further action may be required. Mortality of >50% provides reaso nable assurance that the effluent fails to meet the minimum requirement to disc harge to waters of the state (Rule 62-4.244(3)(a), F.A.C.).



## Algal Growth Potential
The effluent Algal Growth Potential (AGP) was less than the laboratory method detection limit (MDL) of 0.300 mg dry wt/L of the unicellular green alga, Pseudokirchneriella subcapitata , formerly known as Selenastrum capricornutum . Raschke and Shultz (1987) found that AGP values above 5.0 mg dry weight/L represent a “problem” threshold for fresh receiving waters, implying nutrient enrichment. The analytical chemistry suggests that the effluent is phosphorus-limited. The predicted AGP based on inorganic phosphorus concentrations was 2.58 (±20%) mg dry wt/L (Table 1; Miller et al. 1978).

See Table 1 for AGP results.



## Chemistry Results

Total residual chlorine and total ammonia were not de tected in the bioassay sample in the laboratory. Total ammonia was not detected in the sample colle cted and preserved for chemical analysis; thus the unionized ammonia concentration was < 0.02 mg/L.

The effluent dissolved oxygen (DO) concentration (4.9 mg/L, converted from the field measurement of 60% saturation at 25.38o C) violated the Class III freshwater quality criterion of ≥ 5 mg/L (62-302.530(31), F.A.C.). The DO of the bioassay sample as rece ived in the laboratory was 6.6 mg/L at 25.1o C.

Copper and zinc were detected in the effluent at levels that comply with Class III freshwater quality

criteria. Nickel was detected between the labor atory method detection limit (MDL) and practical quantitation limit (PQL).

See Table 2 for results of analytes detected in the effluent, and corresponding limits.

See Appendix 5 for a complete list of chemical analyses performed.



## Conclusion
The effluent sample collected from D-001 for this fac ility on February 13, 2006, was not acutely toxic to either test species during the acute screen 48-hour bi oassays. The effluent AGP result was less than the "problem” threshold for fresh receiving waters. The effluent dissolved oxygen concentration measured in

the field did not meet the Class III freshwater water quality standard.



## Literature Cited
Miller, W. E., T. E. Maloney, and J. C. Greene. 1978. The Selenastrum capricornutum Printz algal assay bottle test. EPA-600/9-78-018. U. S. EPA, Cincinnati, Ohio. 126 p.

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data assessment. J. Wat. Poll. Cont. Fed. 59(4): 222-227.

## 3 Location AGP (measured) Predicted AGP (OP)

± 20% Inorganic N:P ratio Total N:P ratio

Effluent Samples 0.300 U 2.58 ± 0.516 383.33 * Table 1. Measured and predicted algal growth potential (AGP; mg dry weight/L) of the freshwater species
Pseudokirchneriella subcapitata for ortho phosphate (OP) limitation and ratios of nitrogen to phosphorus for samples collected from City of Homestead Municipal Power Plant Once-Through Non-Contact Cooling Water
Discharge on February 13, 2006.

U - Material analyzed for but not detected; value reported is the minimum detection limit
* - N:P ratios could not be calculated because total phosphorus was not detected in the effluent sample

## 4 City of Homestead Municipal Power

Plant NPDES# FL0002721 Class III

Freshwater

Stds Effluent Limits Effluent Samples

None Detected - - -

Aluminum - - 5 U

Arsenic ≤ 50 - 4 U

Cadmium ≤ 2.3 b - 0.05 U
Calcium (mg/L) - - 86.5 A

Chromium-III ≤ 176.4 b - 2 U
Copper ≤ 19.7 b ≤ 19.7 b 13.1 A

Iron ≤ 1,000 ≤ 1,000 10 U

Lead ≤ 9.7 b - 0.075 U
Magnesium (mg/L) - - 5.8 A

Nickel ≤ 109.4 b - 1.9 I

Selenium ≤ 5 - 0.5 U

Silver ≤ 0.07 - 0.025 U

Zinc ≤ 251.5 b ≤ 251.5 b 14 A

Ortho-phosphate - - 0.006 I
Total Phosphorus - - 0.02 U

Ammonia - - 0.01 U

Unionized Ammonia ≤ 0.02 - < 0.02 c

Nitrate+Nitrite - - 2.3
Total Kjeldahl Nitrogen - - 0.24
Organic Nitrogen - - 0.24 c
Total Nitrogen - - 2.54 c

pH (S.U.) 6-8.5 6.5-8.5 7.3
Conductivity (umhos/cm) ≤ 1,275 - 590
Temperature (C) - ≤ 32.2 c 25.4
Dissolved Oxygen (mg/L) ≥ 5 - 4.9 c
Oil and Grease (mg/L) ≤ 5 ≤ 5 1.7 U
Flow (MGD) - 4.11 a 1.35 a
Chlorophyll a (μg/L) - - 0.85 U
Phaeophytin a (μg/L) - - 1.5 U
Alkalinity (mg/L) ≥ 20 - 214
Hardness (mg/L) - Report 239.9 c

Value exceeds the Class III Fresh Water Quality Cr iteria (62-302, F.A.C.) and/or Effluent Limits
a - Annual average

b - Value is calculated based on hardness

c - Calculated value
A - Value reported is the mean of two or more determinations
I - The reported value is between the laboratory method det ection limit and the laboratory practical quantitation limit.
U - Material analyzed for but not detected; value reported is the minimum detection limit General Physical and Chemical Parameters Table 2. Effluent limits, Class III Freshwater Criteria, and chemical data for samples collected from City of Homestead Municipal Power Plant Once-Through Non-Contact Cooling Water Discharge on February 13, 2006.

Organic Constituents ( μ g/L)

Metals ( μ g/L unless otherwise noted)

Nutrients (mg/L)

## 5 Appendix 1. Chai n of Custody form

## 6 Appendix 2. Bench Sheets

## 7 Appendix 2. Bench Sheets (continued)

## 8 Appendix 2. Bench Sheets (continued)

## 9 Appendix 2. Bench Sheets (continued)

## 10 Appendix 2. Bench Sheets (continued)

## 11 Appendix 3. SRT Bench Sheet s and Statistical Analysis

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - Acute Toxicity Ceriodaphnia dubia ( water flea ) - Sodium Chloride ( NaCl )

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

7/20/04 2.60 2.30 0.20 1.90 2.69

8/10/04 2.47 2.29 0.20 1.89 2.69

9/08/04 2.52 2.28 0.21 1.87 2.69

10/05/04 2.13 2.26 0.20 1.85 2.67

11/02/04 2.50 2.27 0.19 1.89 2.65

12/07/04 2.41 2.26 0.19 1.88 2.64

1/04/05 2.47 2.25 0.18 1.88 2.61

2/01/05 2.22 2.23 0.18 1.87 2.59

3/01/05 2.35 2.23 0.17 1.89 2.57

3/29/05 2.37 2.22 0.16 1.91 2.54

4/26/05 2.00 2.21 0.17 1.87 2.55

5/24/05 1.94 2.23 0.19 1.85 2.61

6/01/05 3.29 2.26 0.29 1.69 2.84

6/07/05 2.12 2.15 0.29 1.57 2.73

7/06/05 2.25 2.15 0.28 1.58 2.72

8/02/05 2.11 2.14 0.29 1.56 2.71

9/12/05 2.06 2.14 0.29 1.55 2.73

10/4/05 2.08 2.33 0.30 1.73 2.93

11/01/05 2.40 2.33 0.30 1.73 2.93

11/29/05 2.09 2.32 0.30 1.72 2.92

01/05/06 2.27 2.30 0.30 1.70 2.90

01/31/06 1.94 2.28 0.30 1.68 2.88

Means are of the last 20 SRT tests.

Lower Confidence Limit = mean - 2 * Std Dev CV = 13.20
Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 29%



## Standard Reference Toxicant Data Ceriodaphnia dubia LC50

1.50 2.00 2.50 3.00 3.50

9/08/04 10/05/0 4

1 1 / 02

/ 0 4

1 2 /0

7 /04

1/0

4 /0

5

2 / 0 1/

0 5

3 /01/05 3/2

9 /0

5

4 / 2 6/

0 5

5 /24/05 6/01/05 6/0

7 /0

5

7 / 0 6/

0 5 8/02/05 9/1

2 /0

5

1 0 / 4/

0 5

1 1 /0

1 /05 11/29/05 01/05/0 6

0 1 / 31

/ 0 6



## Test Datemg/L NaCl



### LC50 Mean LC50 Lower Control Limit Upper Control Limit

## 12 Appendix 3. (continued)

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant ( SRT ) Test Data - A cute Toxicit y C

yp rinella leedsi ( bannerfin shiner ) - Sodium Chloride ( NaCl )

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

05/24/04 7.42 7.88 1.20 5.49 10.27

06/22/04 9.19 7.91 1.23 5.46 10.36

07/22/04 9.23 7.93 1.24 5.45 10.41

08/10/04 9.65 7.95 1.27 5.41 10.49

09/08/04 8.85 7.91 1.22 5.46 10.36

10/05/04 7.43 7.83 1.20 5.43 10.24

11/02/04 8.31 7.93 1.15 5.63 10.24

11/30/04 8.53 7.94 1.15 5.63 10.24

01/04/05 7.86 7.87 1.11 5.65 10.08

02/01/05 7.22 7.74 1.03 5.68 9.80

03/01/05 9.54 7.87 1.09 5.68 10.05

03/29/05 8.04 7.99 0.96 6.07 9.91

04/26/05 7.38 8.03 0.90 6.22 9.84

05/24/05 8.41 8.11 0.86 6.40 9.83

07/12/05 7.59 8.08 0.86 6.35 9.81

08/02/05 7.53 8.13 0.80 6.53 9.72

09/13/05 7.84 8.16 0.77 6.63 9.70

10/04/05 7.81 8.16 0.77 6.62 9.69

11/01/05 7.74 8.15 0.77 6.61 9.69

11/29/05 7.49 8.15 0.77 6.61 9.69

01/04/06 7.94 8.18 0.75 6.68 9.68

01/24/06 8.39 8.14 0.74 6.66 9.62

Means are of the last 20 SRT tests.

Lower Confidence Limit = mean - 2 * Std Dev CV = 9.09
Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 19%



## Standard Reference Toxicant Data Cyprinella leedsi LC50

4 6 8 10 1207/22/0408/10/0409/08/0410/05/0411/02/0411/30/0401/04/0502/01/0503/01/0503/29/0504/26/0505/24/0507/12/0508/02/0509/13/0510/04/0511/01/0511/29/0501/04/0601/24/06



### Test Datemg/L NaCl



### LC50 Mean LC50 Lower Control Limit Upper Control Limit

## 13 Appendix 3. (continued)

## 14 Appendix 3. (continued)

## 15 Appendix 3. (continued)

## 16 Appendix 3. (continued)

## 17 Appendix 3. (continued)

Ceriodaphnia dubia Acute Standard Reference Toxicant Test

START DATE: 01/31/06 END DATE: 02/04/06 DURATION: 96 H

TOXICANT : NaCl

RAW DATA: Concentration Number Mortalities --- ---- (ug/L) Exposed

0.00 20 0

1.50 20 0

2.00 20 11

2.50 20 20

3.00 20 20

4.00 20 20

SPEARMAN-KARBER TRIM: .00%

SPEARMAN-KARBER ESTIMATES: LC50: 1.94*

95% LOWER CONFIDENCE: 1.84

95% UPPER CONFIDENCE: 2.06

________________________ __________________ ______________________

Cyprinella leedsi Acute Standard Reference Toxicant Test

START DATE: 01/24/06 EN D DATE: 01/28/06 DURATION: 96 H

TOXICANT : NaCl

RAW DATA: Concentration Number Mortalities (ug/L) Exposed

0.00 20 0

6.00 20 1

7.00 20 1

8.00 20 7

9.00 20 14

10.00 20 19

SPEARMAN-KARBER TRIM: 5.00%

SPEARMAN-KARBER ESTIMATES: LC50: 8.39*

95% LOWER CONFIDENCE: 8.06

95% UPPER CONFIDENCE: 8.74

* LC50 calculated at 48 hours to match environmental test duration

## 18 Appendix 4. Test Organism Receipts

## 19 Appendix 4. (continued)20Appendix 5. Chemical Analyses performed on the effluent from the Cityof Homestead Municipal Power PlantOnce-Through Non-Contact Cooling Water DischargeOutfall D-001, sampled on February 13, 2006.Date Sampled Field ID Analysis Component Result Units Remark MDL PQL2/13/2006 10:00 51-HPP-1 Bio-AGP/LimNut AlgalGrowth Potential 0.3 mg DryWt/L U 0.3 0.92/13/2006 10:00 51-HPP-1 Bio-Chl-a Chlorophyll-A, Monochromatic, Water 0.85 ug/L U 0.85 2.62/13/2006 10:00 51-HPP-1 Bio-Chl-a Phaeophytin-A,Monochromatic, Water 1.5 ug/L U 1.5 4.52/13/2006 10:00 51-HPP-1 Bio-Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 LC50 L2/13/2006 10:00 51-HPP-1 Bio-Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 100 LC50 L2/13/2006 10:00 51-HPP-1 BNA-Water 1,2,4-Trichlorobenzene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 1,2-Dichlorobenzene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 1,3-Dichlorobenzene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 1,4-Dichlorobenzene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 2,4,6-Trichlorophenol 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 2,4-Dichlorophenol 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 2,4-Dimethylphenol 48 ug/L U 48 1902/13/2006 10:00 51-HPP-1 BNA-Water2,4-Dinitrophenol 14 ug/L U 14 582/13/2006 10:00 51-HPP-1 BNA-Water 2,4-Dinitrotoluene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 2,6-Dinitrotoluene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 2-Chloronaphthalene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 2-Chlorophenol 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 2-Methyl-4,6-dinitrophenol 2.9 ug/L U 2.9 122/13/2006 10:00 51-HPP-1 BNA-Water2-Nitrophenol 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 3,3'-Dichlorobenzidine 38 ug/L U 38 1502/13/2006 10:00 51-HPP-1 BNA-Water 4,4'-DDD 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water 4,4'-DDE 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water 4,4'-DDT 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water 4-Bromophenyl phenyl ether 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 4-Chloro-3-methylphenol 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water 4-Chlorophenyl phenyl ether 1.9 ug/L U 1.9 7.72/13/2006 10:00 51-HPP-1 BNA-Water 4-Nitrophenol 14 ug/L U 14 582/13/2006 10:00 51-HPP-1 BNA-WaterAcenaphthene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-WaterAcenaphthylene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Aldrin 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Wateralpha-BHC 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-WaterAnthracene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Benzidine 96 ug/L U 96 3802/13/2006 10:00 51-HPP-1 BNA-Water Benzo(a)anthracene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Benzo(a)pyrene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Benzo(b)fluoranthene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Benzo(g,h,i)perylene 0.96 ug/L U 0.96 3.821Appendix 5. Chemical Analyses (continued)Date Sampled Field ID Analysis Component Result Units Remark MDL PQL2/13/2006 10:00 51-HPP-1 BNA-Water Benzo(k)fluoranthene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water beta-BHC 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-chloroethoxy)methane 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-chloroethyl)ether 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-chloroisopropyl)ether 2.9 ug/L U 2.9 122/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-ethylhexyl)phthalate 14 ug/L UJ 14 582/13/2006 10:00 51-HPP-1 BNA-Water Butyl benzyl phthalate 4.8 ug/L U 4.8 192/13/2006 10:00 51-HPP-1 BNA-WaterChrysene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water delta-BHC 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water Dibenzo(a,h)anthracene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-WaterDieldrin 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water Diethyl phthalate 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Dimethyl phthalate 48 ug/L U 48 1902/13/2006 10:00 51-HPP-1 BNA-Water Di-n-butyl phthalate 4.8 ug/L U 4.8 192/13/2006 10:00 51-HPP-1 BNA-Water Di-n-octyl phthalate 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-WaterEndosulfan I 3.8 ug/L U 3.8 152/13/2006 10:00 51-HPP-1 BNA-WaterEndosulfan II 3.8 ug/L U 3.8 152/13/2006 10:00 51-HPP-1 BNA-Water Endosulfan sulfate 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water Endrin 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-WaterEndrin aldehyde 3.8 ug/L U 3.8 152/13/2006 10:00 51-HPP-1 BNA-WaterFluoranthene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Fluorene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water gamma-BHC 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-WaterHeptachlor 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water Heptachlor epoxide 1.4 ug/L U 1.4 5.82/13/2006 10:00 51-HPP-1 BNA-Water Hexachlorobenzene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Hexachlorobutadiene 2.9 ug/L U 2.9 122/13/2006 10:00 51-HPP-1 BNA-Water Hexachlorocyclopentadiene 2.9 ug/L U 2.9 122/13/2006 10:00 51-HPP-1 BNA-WaterHexachloroethane 2.9 ug/L U 2.9 122/13/2006 10:00 51-HPP-1 BNA-Water Indeno(1,2,3-cd)pyrene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-WaterIsophorone 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-WaterNaphthalene 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-WaterNitrobenzene 1.9 ug/L U 1.9 7.72/13/2006 10:00 51-HPP-1 BNA-Water N-Nitrosodimethylamine 1.9 ug/L U 1.9 7.72/13/2006 10:00 51-HPP-1 BNA-Water N-Nitrosodi-n-propylamine 1.9 ug/L U 1.9 7.72/13/2006 10:00 51-HPP-1 BNA-Water N-Nitrosodiphenylamine 2.9 ug/L U 2.9 122/13/2006 10:00 51-HPP-1 BNA-Water Pentachlorophenol 2.9 ug/L U 2.9 122/13/2006 10:00 51-HPP-1 BNA-WaterPhenanthrene 0.96 ug/L U 0.96 3.822Appendix 5. Chemical Analyses (continued)Date Sampled Field ID Analysis Component Result Units Remark MDL PQL2/13/2006 10:00 51-HPP-1 BNA-Water Phenol 0.96 ug/L U 0.96 3.82/13/2006 10:00 51-HPP-1 BNA-Water Pyrene 0.96 ug/L U 0.96 3.82/13/2006 10:15 BLANK BNA-Water 1,2,4-Trichlorobenzene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 1,2-Dichlorobenzene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 1,3-Dichlorobenzene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 1,4-Dichlorobenzene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 2,4,6-Trichlorophenol 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 2,4-Dichlorophenol 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 2,4-Dimethylphenol 49 ug/L U 49 1902/13/2006 10:15 BLANK BNA-Water 2,4-Dinitrophenol 15 ug/L U 15 582/13/2006 10:15 BLANK BNA-Water 2,4-Dinitrotoluene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 2,6-Dinitrotoluene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 2-Chloronaphthalene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 2-Chlorophenol 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 2-Methyl-4,6-dinitrophenol 2.9 ug/L U 2.9 122/13/2006 10:15 BLANK BNA-Water 2-Nitrophenol 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 3,3'-Dichlorobenzidine 39 ug/L U 39 1602/13/2006 10:15 BLANK BNA-Water4,4'-DDD 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water4,4'-DDE 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water4,4'-DDT 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water 4-Bromophenyl phenyl ether 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 4-Chloro-3-methylphenol 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water 4-Chlorophenyl phenyl ether 1.9 ug/L U 1.9 7.82/13/2006 10:15 BLANK BNA-Water 4-Nitrophenol 15 ug/L U 15 582/13/2006 10:15 BLANK BNA-Water Acenaphthene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Acenaphthylene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Aldrin 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water alpha-BHC 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Anthracene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Benzidine 97 ug/L U 97 3902/13/2006 10:15 BLANK BNA-Water Benzo(a)anthracene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Benzo(a)pyrene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Benzo(b)fluoranthene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Benzo(g,h,i)perylene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Benzo(k)fluoranthene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water beta-BHC 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Bis(2-chloroethoxy)methane 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Bis(2-chloroethyl)ether 0.97 ug/L U 0.97 3.923Appendix 5. Chemical Analyses (continued)Date Sampled Field ID Analysis Component Result Units Remark MDL PQL2/13/2006 10:15 BLANK BNA-Water Bis(2-chloroisopropyl)ether 2.9 ug/L U 2.9 122/13/2006 10:15 BLANK BNA-Water Bis(2-ethylhexyl)phthalate 15 ug/L UJ 15 582/13/2006 10:15 BLANK BNA-Water Butyl benzyl phthalate 4.9 ug/L U 4.9 192/13/2006 10:15 BLANK BNA-WaterChrysene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water delta-BHC 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Dibenzo(a,h)anthracene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Dieldrin 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Diethyl phthalate 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Dimethyl phthalate 49 ug/L U 49 1902/13/2006 10:15 BLANK BNA-Water Di-n-butyl phthalate 4.9 ug/L U 4.9 192/13/2006 10:15 BLANK BNA-Water Di-n-octyl phthalate 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-WaterEndosulfan I 3.9 ug/L U 3.9 162/13/2006 10:15 BLANK BNA-WaterEndosulfan II 3.9 ug/L U 3.9 162/13/2006 10:15 BLANK BNA-Water Endosulfan sulfate 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Endrin 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Endrin aldehyde 3.9 ug/L U 3.9 162/13/2006 10:15 BLANK BNA-Water Fluoranthene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Fluorene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water gamma-BHC 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Heptachlor 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Heptachlor epoxide 1.5 ug/L U 1.5 5.82/13/2006 10:15 BLANK BNA-Water Hexachlorobenzene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Hexachlorobutadiene 2.9 ug/L U 2.9 122/13/2006 10:15 BLANK BNA-Water Hexachlorocyclopentadiene 2.9 ug/L U 2.9 122/13/2006 10:15 BLANK BNA-Water Hexachloroethane 2.9 ug/L U 2.9 122/13/2006 10:15 BLANK BNA-Water Indeno(1,2,3-cd)pyrene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Isophorone 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Naphthalene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Nitrobenzene 1.9 ug/L U 1.9 7.82/13/2006 10:15 BLANK BNA-Water N-Nitrosodimethylamine 1.9 ug/L U 1.9 7.82/13/2006 10:15 BLANK BNA-Water N-Nitrosodi-n-propylamine 1.9 ug/L U 1.9 7.82/13/2006 10:15 BLANK BNA-Water N-Nitrosodiphenylamine 2.9 ug/L U 2.9 122/13/2006 10:15 BLANK BNA-Water Pentachlorophenol 2.9 ug/L U 2.9 122/13/2006 10:15 BLANK BNA-WaterPhenanthrene 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Phenol 0.97 ug/L U 0.97 3.92/13/2006 10:15 BLANK BNA-Water Pyrene 0.97 ug/L U 0.97 3.92/13/2006 10:00 51-HPP-1 GC-WaterAlachlor 0.6 ug/L U 0.6 2.42/13/2006 10:00 51-HPP-1 GC-WaterAmetryn 0.05 ug/L U 0.05 0.224Appendix 5. Chemical Analyses (continued)Date Sampled Field ID Analysis Component Result Units Remark MDL PQL2/13/2006 10:00 51-HPP-1 GC-WaterAtrazine 0.05 ug/L U 0.05 0.22/13/2006 10:00 51-HPP-1 GC-Water Azinphos Methyl 0.2 ug/L U 0.2 0.82/13/2006 10:00 51-HPP-1 GC-WaterBromacil 0.2 ug/L U 0.2 0.82/13/2006 10:00 51-HPP-1 GC-WaterButylate 0.2 ug/L U 0.2 0.82/13/2006 10:00 51-HPP-1 GC-Water Chlorpyrifos Ethyl 0.05 ug/L U 0.05 0.22/13/2006 10:00 51-HPP-1 GC-Water Chlorpyrifos Methyl 0.1 ug/L U 0.1 0.42/13/2006 10:00 51-HPP-1 GC-WaterDiazinon 0.05 ug/L U 0.05 0.22/13/2006 10:00 51-HPP-1 GC-WaterEthion 0.05 ug/L U 0.05 0.22/13/2006 10:00 51-HPP-1 GC-WaterEthoprop 0.1 ug/L U 0.1 0.42/13/2006 10:00 51-HPP-1 GC-WaterFenamiphos 0.2 ug/L U 0.2 0.82/13/2006 10:00 51-HPP-1 GC-WaterFonofos 0.1 ug/L U 0.1 0.42/13/2006 10:00 51-HPP-1 GC-WaterHexazinone 0.1 ug/L U 0.1 0.42/13/2006 10:00 51-HPP-1 GC-WaterMalathion 0.15 ug/L UJ 0.15 0.62/13/2006 10:00 51-HPP-1 GC-WaterMetalaxyl 0.25 ug/L U 0.25 12/13/2006 10:00 51-HPP-1 GC-WaterMetolachlor 0.5 ug/L U 0.5 22/13/2006 10:00 51-HPP-1 GC-WaterMetribuzin 0.1 ug/L U 0.1 0.42/13/2006 10:00 51-HPP-1 GC-WaterMevinphos 0.2 ug/L U 0.2 0.82/13/2006 10:00 51-HPP-1 GC-WaterNaled 0.8 ug/L U 0.8 3.22/13/2006 10:00 51-HPP-1 GC-WaterNorflurazon 0.15 ug/L U 0.15 0.62/13/2006 10:00 51-HPP-1 GC-Water Parathion Ethyl 0.15 ug/L U 0.15 0.62/13/2006 10:00 51-HPP-1 GC-Water Parathion Methyl 0.1 ug/L U 0.1 0.42/13/2006 10:00 51-HPP-1 GC-WaterPhorate 0.05 ug/L U 0.05 0.22/13/2006 10:00 51-HPP-1 GC-WaterPrometryn 0.15 ug/L U 0.15 0.62/13/2006 10:00 51-HPP-1 GC-WaterSimazine 0.05 ug/L U 0.05 0.22/13/2006 10:15 BLANK GC-WaterAlachlor 0.58 ug/L U 0.58 2.32/13/2006 10:15 BLANK GC-WaterAmetryn 0.048 ug/L U 0.048 0.192/13/2006 10:15 BLANK GC-WaterAtrazine 0.048 ug/L U 0.048 0.192/13/2006 10:15 BLANK GC-Water Azinphos Methyl 0.19 ug/L U 0.19 0.762/13/2006 10:15 BLANK GC-WaterBromacil 0.19 ug/L U 0.19 0.762/13/2006 10:15 BLANK GC-WaterButylate 0.19 ug/L U 0.19 0.762/13/2006 10:15 BLANK GC-Water Chlorpyrifos Ethyl 0.048 ug/L U 0.048 0.192/13/2006 10:15 BLANK GC-Water Chlorpyrifos Methyl 0.096 ug/L U 0.096 0.382/13/2006 10:15 BLANK GC-WaterDiazinon 0.048 ug/L U 0.048 0.192/13/2006 10:15 BLANK GC-WaterEthion 0.048 ug/L U 0.048 0.192/13/2006 10:15 BLANK GC-WaterEthoprop 0.096 ug/L U 0.096 0.382/13/2006 10:15 BLANK GC-WaterFenamiphos 0.19 ug/L U 0.19 0.762/13/2006 10:15 BLANK GC-WaterFonofos 0.096 ug/L U 0.096 0.382/13/2006 10:15 BLANK GC-WaterHexazinone 0.096 ug/L U 0.096 0.3825Appendix 5. Chemical Analyses (continued)Date Sampled Field ID Analysis Component Result Units Remark MDL PQL2/13/2006 10:15 BLANK GC-Water Malathion 0.14 ug/L UJ 0.14 0.562/13/2006 10:15 BLANK GC-WaterMetalaxyl 0.24 ug/L U 0.24 0.962/13/2006 10:15 BLANK GC-Water Metolachlor 0.48 ug/L U 0.48 1.92/13/2006 10:15 BLANK GC-Water Metribuzin 0.096 ug/L U 0.096 0.382/13/2006 10:15 BLANK GC-WaterMevinphos 0.19 ug/L U 0.19 0.762/13/2006 10:15 BLANK GC-WaterNaled 0.77 ug/L U 0.77 3.12/13/2006 10:15 BLANK GC-Water Norflurazon 0.14 ug/L U 0.14 0.562/13/2006 10:15 BLANK GC-Water Parathion Ethyl 0.14 ug/L U 0.14 0.562/13/2006 10:15 BLANK GC-Water Parathion Methyl 0.096 ug/L U 0.096 0.382/13/2006 10:15 BLANK GC-WaterPhorate 0.048 ug/L U 0.048 0.192/13/2006 10:15 BLANK GC-WaterPrometryn 0.14 ug/L U 0.14 0.562/13/2006 10:15 BLANK GC-WaterSimazine 0.048 ug/L U 0.048 0.192/13/2006 10:00 51-HPP-1 Metals-Water Aluminum 5 ug/L U 5 202/13/2006 10:00 51-HPP-1 Metals-Water Arsenic 4 ug/L U 4 162/13/2006 10:00 51-HPP-1 Metals-Water Cadmium 0.05 ug/L U 0.05 0.22/13/2006 10:00 51-HPP-1 Metals-Water Calcium 86.5 mg/L A 0.05 0.22/13/2006 10:00 51-HPP-1 Metals-Water Chromium 2 ug/L U 2 82/13/2006 10:00 51-HPP-1 Metals-Water Copper 13.1 ug/L A 0.25 12/13/2006 10:00 51-HPP-1 Metals-Water Iron 10 ug/L U 10 402/13/2006 10:00 51-HPP-1 Metals-Water Lead 0.075 ug/L U 0.075 0.32/13/2006 10:00 51-HPP-1 Metals-WaterMagnesium 5.8 mg/L A 0.01 0.042/13/2006 10:00 51-HPP-1 Metals-Water Nickel 1.9 ug/L I 1 42/13/2006 10:00 51-HPP-1 Metals-Water Selenium 0.5 ug/L U 0.5 22/13/2006 10:00 51-HPP-1 Metals-Water Silver 0.025 ug/L U 0.025 0.12/13/2006 10:00 51-HPP-1 Metals-Water Zinc 14 ug/L A 3 122/13/2006 10:15 BLANK Metals-Water Aluminum 5 ug/L U 5 202/13/2006 10:15 BLANK Metals-Water Arsenic 4 ug/L U 4 162/13/2006 10:15 BLANK Metals-WaterCadmium 0.05 ug/L U 0.05 0.22/13/2006 10:15 BLANK Metals-WaterCalcium 0.05 mg/L U 0.05 0.22/13/2006 10:15 BLANK Metals-Water Chromium 2 ug/L U 2 82/13/2006 10:15 BLANK Metals-Water Copper 0.25 ug/L U 0.25 12/13/2006 10:15 BLANK Metals-Water Iron 10 ug/L U 10 402/13/2006 10:15 BLANK Metals-Water Lead 0.075 ug/L U 0.075 0.32/13/2006 10:15 BLANK Metals-WaterMagnesium 0.01 mg/L U 0.01 0.042/13/2006 10:15 BLANK Metals-Water Nickel 1 ug/L U 1 42/13/2006 10:15 BLANK Metals-WaterSelenium 0.5 ug/L U 0.5 22/13/2006 10:15 BLANK Metals-WaterSilver 0.025 ug/L U 0.025 0.12/13/2006 10:15 BLANK Metals-Water Zinc 3 ug/L U 3 1226Appendix 5. Chemical Analyses (continued)Date Sampled Field ID Analysis Component Result Units Remark MDL PQL2/13/2006 10:00 51-HPP-1 Nutrients-LiquidAlkalinity 214 mg CaCO3/L 0.65 2.52/13/2006 10:00 51-HPP-1 Nutrients-LiquidAmmonia-N 0.01 mg N/L U 0.01 0.022/13/2006 10:00 51-HPP-1 Nutrients-LiquidKjeldahl Nitrogen 0.24 mg N/L 0.08 0.22/13/2006 10:00 51-HPP-1 Nutrients-Liquid NO2NO3-N 2.3 mg N/L 0.04 0.12/13/2006 10:00 51-HPP-1 Nutrients-LiquidO-Phosphate-P 0.006 mg P/L I 0.004 0.012/13/2006 10:00 51-HPP-1 Nutrients-Liquid Total-P 0.02 mg P/L U 0.02 0.062/13/2006 10:15 BLANK Nutrients-LiquidAmmonia-N 0.01 mg N/L U 0.01 0.022/13/2006 10:15 BLANK Nutrients-Liquid Kjeldahl Nitrogen 0.08 mg N/L U 0.08 0.22/13/2006 10:15 BLANK Nutrients-Liquid NO2NO3-N 0.005 mg N/L I 0.004 0.012/13/2006 10:15 BLANK Nutrients-Liquid O-Phosphate-P 0.004 mg P/L U 0.004 0.012/13/2006 10:15 BLANK Nutrients-Liquid Total-P 0.02 mg P/L U 0.02 0.062/13/2006 10:00 51-HPP-1 Overflow Oil and Grease 1.7 mg/L U2/13/2006 10:15 BLANK Overflow Oil and Grease 1.7 mg/L U

## 27 Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 7 2 1 11 12 0 6 0 2 1 3 18 B 19 S 20 1

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 7 2 1 11 12 0 6 0 2 1 3 18 X 19 S 20 1

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 7 2 1 11 12 0 6 0 2 1 3 18 S 19 S 20 1

66 

# Biological Analyses of City of Homestead Municipal Power Plant Once-Through Non-Contact Coolin g Water Discharge effluent
sampled on February 13 , 2006. NPDES #FL0002721.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

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# The Bioassay of City of Homestead Municipal Power Plant OnceThrough Non-Contact Cooling Water Discharge effluent sampled
on February 13, 20 06. NPDES #FL0002721.

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# The Priority Pollutants Analysis of City of Homestead Municipal Power Plant Once-Through Non-Co ntact Cooling Water Discharge effluent sampled on February 13, 2006. NP DES #FL0002721.