Bioassays of the City of 

Homestead Municipal Power Plant 

Miami-Dade County 

NPDES #FL0002721 

Sampled February 13, 2006 

April 2006

Biology Section 

Bureau of Laboratories 

Division of Resource Assessment and Management 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1

City of Homestead Municipal Power Plant, 675 N. Flagler Avenue, Homestead, Miami-Dade County, 

Florida, NPDES #FL0002721. Effluent samples for this facility were collected on February 13, 2006. 

Introduction 

The City of Homestead Municipal Power Plant is an electric power generating plant having a peak electric 

power generating capacity of 53 megawatts. The facility has a design flow of 4.11 million gallons per day 

(MGD). The facility had an average annual daily flow (AADF) of 1.35 MGD from February 2005 through 

January 2006. The facility has 16 generating units, of which two units are cooled by an air cooling system, 

two units are cooled by a mechanical-draft cooling tower system and 12 units are cooled by once-through 

cooling water supplied from on-site wells. Chemical additives are not added to this cooling water. This 

once-through cooling water is passed through an on-site double oil-water separator treatment system 

prior to discharge through Outfall D-001 to a pipe (approximately 624 m [2050 feet] long) which 

discharges into the Class III freshwaters of an unnamed drainage ditch that runs into the C-103S Canal, 

thence into Biscayne Bay. The origin of the outfall pipeline, or D-001, is located approximately at latitude 

25o 28’ 27” N, longitude 80o 28’ 12” W. The point where the pipeline ends at the unnamed ditch is located 

approximately at latitude 25o 28’ 08” N, longitude 80o 28’ 11” W. 

The facility is not currently under any Administrative Orders (AO) or Consent Orders (CO). The facility has 

no toxicity testing requirements or mixing zones. During the past year, the facility had no permit violations. 

(Facility information provided by Terry Davis of FDEP Southeast District). 

Methods 

Samples were collected from the EFF-01 NPDES sampling point for Outfall D-001 by Terry Davis (FDEP 

Southeast District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOP’s are 

available on the web at: http://www.floridadep.org/labs/qa/sops.htm. 

See Appendix 1 for the completed chain of custody form. 

The 48-hour acute screening toxicity tests and algal growth potential test were performed following 

internal DEP SOP’s TA07_01, TA07_02, and TA08_01 through TA08_09. All internal DEP SOP’s are 

available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. The Department uses the 

48-hour acute screening toxicity test to provide reasonable assurance that the facility does not adversely 

affect waters of the state. 

Toxicity Test Results 

Tests were performed February 14 - 16, 2006. 

Ceriodaphnia dubia 48-hour acute screening bioassay – LC50 > 100%, 0% mortality* in 100% sample at 

 48 hours 

Cyprinella leedsi 48-hour acute screening bioassay – LC50 > 100%, 0% mortality* in 100% sample 

 at 48 hours 

The bioassay sample was not acutely toxic to the test organisms Ceriodaphnia dubia and Cyprinella 

leedsi. 

See Appendix 2 for bioassay bench sheets. 

See Appendix 3 for results of the last 20 standard reference toxicant (SRT) tests and bench sheets and 

statistical analyses of the SRT test that corresponds to this facility test. See Appendix 4 for test organism 

receipts. 
2

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides reasonable 

assurance that the effluent meets the acute toxicity criterion of Florida’s Surface Water Quality Standards 

(Rule 62-302.500 (1)(a)4, F.A.C.). Mortality between 20-50% mortality indicates low to moderate levels of 

toxicity, and further action may be required. Mortality of >50% provides reasonable assurance that the 

effluent fails to meet the minimum requirement to discharge to waters of the state (Rule 62-4.244(3)(a), 

F.A.C.). 

Algal Growth Potential 

The effluent Algal Growth Potential (AGP) was less than the laboratory method detection limit (MDL) of 

0.300 mg dry wt/L of the unicellular green alga, Pseudokirchneriella subcapitata, formerly known as 

Selenastrum capricornutum. Raschke and Shultz (1987) found that AGP values above 5.0 mg dry 

weight/L represent a “problem” threshold for fresh receiving waters, implying nutrient enrichment. The 

analytical chemistry suggests that the effluent is phosphorus-limited. The predicted AGP based on 

inorganic phosphorus concentrations was 2.58 (±20%) mg dry wt/L (Table 1; Miller et al. 1978). 

See Table 1 for AGP results. 

Chemistry Results 

Total residual chlorine and total ammonia were not detected in the bioassay sample in the laboratory. 

Total ammonia was not detected in the sample collected and preserved for chemical analysis; thus the 

unionized ammonia concentration was < 0.02 mg/L. 

The effluent dissolved oxygen (DO) concentration (4.9 mg/L, converted from the field measurement of 

60% saturation at 25.38o C) violated the Class III freshwater quality criterion of ≥ 5 mg/L (62-302.530(31), 

F.A.C.). The DO of the bioassay sample as received in the laboratory was 6.6 mg/L at 25.1o C. 

Copper and zinc were detected in the effluent at levels that comply with Class III freshwater quality 

criteria. Nickel was detected between the laboratory method detection limit (MDL) and practical 

quantitation limit (PQL). 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. 

 

See Appendix 5 for a complete list of chemical analyses performed. 

Conclusion 

The effluent sample collected from D-001 for this facility on February 13, 2006, was not acutely toxic to 

either test species during the acute screen 48-hour bioassays. The effluent AGP result was less than the 

"problem” threshold for fresh receiving waters. The effluent dissolved oxygen concentration measured in 

the field did not meet the Class III freshwater water quality standard. 

Literature Cited 

Miller, W. E., T. E. Maloney, and J. C. Greene. 1978. The Selenastrum capricornutum Printz algal 

assay bottle test. EPA-600/9-78-018. U. S. EPA, Cincinnati, Ohio. 126 p. 

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data assessment. 

J. Wat. Poll. Cont. Fed. 59(4): 222-227. 
3

Location AGP (measured) Predicted AGP (OP) 

± 20% Inorganic N:P ratio Total N:P ratio

Effluent Samples 0.300 U 2.58 ± 0.516 383.33 *

Table 1. Measured and predicted algal growth potential (AGP; mg dry weight/L) of the freshwater species 

Pseudokirchneriella subcapitata for ortho phosphate (OP) limitation and ratios of nitrogen to phosphorus for 

samples collected from City of Homestead Municipal Power Plant Once-Through Non-Contact Cooling Water 

Discharge on February 13, 2006.

U - Material analyzed for but not detected; value reported is the minimum detection limit

* - N:P ratios could not be calculated because total phosphorus was not detected in the effluent sample 
4

City of Homestead Municipal Power 

Plant NPDES# FL0002721

Class III 

Freshwater 

Stds

Effluent Limits Effluent 

Samples

None Detected - - -

Aluminum - - 5 U

Arsenic ≤ 50 - 4 U

Cadmium ≤ 2.3 b - 0.05 U

Calcium (mg/L) - - 86.5 A

Chromium-III ≤ 176.4 b - 2 U

Copper ≤ 19.7 b ≤ 19.7 b 13.1 A

Iron ≤ 1,000 ≤ 1,000 10 U

Lead ≤ 9.7 b - 0.075 U

Magnesium (mg/L) - - 5.8 A

Nickel ≤ 109.4 b - 1.9 I

Selenium ≤ 5 - 0.5 U

Silver ≤ 0.07 - 0.025 U

Zinc ≤ 251.5 b ≤ 251.5 b 14 A

Ortho-phosphate - - 0.006 I

Total Phosphorus - - 0.02 U

Ammonia - - 0.01 U

Unionized Ammonia ≤ 0.02 - < 0.02 c

Nitrate+Nitrite - - 2.3

Total Kjeldahl Nitrogen - - 0.24

Organic Nitrogen - - 0.24 c

Total Nitrogen - - 2.54 c

pH (S.U.) 6-8.5 6.5-8.5 7.3

Conductivity (umhos/cm) ≤ 1,275 - 590

Temperature (C) - ≤ 32.2 c 25.4

Dissolved Oxygen (mg/L) ≥ 5 - 4.9 c

Oil and Grease (mg/L) ≤ 5 ≤ 5 1.7 U

Flow (MGD) - 4.11 a 1.35 a

Chlorophyll a (μg/L) - - 0.85 U

Phaeophytin a (μg/L) - - 1.5 U

Alkalinity (mg/L) ≥ 20 - 214

Hardness (mg/L) - Report 239.9 c

Value exceeds the Class III Fresh Water Quality Criteria (62-302, F.A.C.) and/or Effluent Limits

a - Annual average

b - Value is calculated based on hardness

c - Calculated value

A - Value reported is the mean of two or more determinations

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit.

U - Material analyzed for but not detected; value reported is the minimum detection limit

General Physical and Chemical Parameters

Table 2. Effluent limits, Class III Freshwater Criteria, and chemical data for samples 

collected from City of Homestead Municipal Power Plant Once-Through Non-Contact 

Cooling Water Discharge on February 13, 2006.

Organic Constituents (μg/L)

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)
5

Appendix 1. Chain of Custody form 
6

Appendix 2. Bench Sheets 
7

Appendix 2. Bench Sheets (continued) 
8

Appendix 2. Bench Sheets (continued) 
9

Appendix 2. Bench Sheets (continued) 
10

Appendix 2. Bench Sheets (continued) 
11

Appendix 3. SRT Bench Sheets and Statistical Analysis 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - Acute Toxicity

Ceriodaphnia dubia (water flea) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

7/20/04 2.60 2.30 0.20 1.90 2.69

8/10/04 2.47 2.29 0.20 1.89 2.69

9/08/04 2.52 2.28 0.21 1.87 2.69

10/05/04 2.13 2.26 0.20 1.85 2.67

11/02/04 2.50 2.27 0.19 1.89 2.65

12/07/04 2.41 2.26 0.19 1.88 2.64

1/04/05 2.47 2.25 0.18 1.88 2.61

2/01/05 2.22 2.23 0.18 1.87 2.59

3/01/05 2.35 2.23 0.17 1.89 2.57

3/29/05 2.37 2.22 0.16 1.91 2.54

4/26/05 2.00 2.21 0.17 1.87 2.55

5/24/05 1.94 2.23 0.19 1.85 2.61

6/01/05 3.29 2.26 0.29 1.69 2.84

6/07/05 2.12 2.15 0.29 1.57 2.73

7/06/05 2.25 2.15 0.28 1.58 2.72

8/02/05 2.11 2.14 0.29 1.56 2.71

9/12/05 2.06 2.14 0.29 1.55 2.73

10/4/05 2.08 2.33 0.30 1.73 2.93

11/01/05 2.40 2.33 0.30 1.73 2.93

11/29/05 2.09 2.32 0.30 1.72 2.92

01/05/06 2.27 2.30 0.30 1.70 2.90

01/31/06 1.94 2.28 0.30 1.68 2.88

Means are of the last 20 SRT tests.

Lower Confidence Limit = mean - 2 * Std Dev CV = 13.20

Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 29%

Standard Reference Toxicant Data 

Ceriodaphnia dubia LC50

1.50

2.00

2.50

3.00

3.50

9/08/04

10/05/04

11/02/04

12/07/04

1/04/05

2/01/05

3/01/05

3/29/05

4/26/05

5/24/05

6/01/05

6/07/05

7/06/05

8/02/05

9/12/05

10/4/05

11/01/05

11/29/05

01/05/06

01/31/06

Test Date

mg/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit
12

Appendix 3. (continued) 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - Acute Toxicity

Cyprinella leedsi (bannerfin shiner) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

05/24/04 7.42 7.88 1.20 5.49 10.27

06/22/04 9.19 7.91 1.23 5.46 10.36

07/22/04 9.23 7.93 1.24 5.45 10.41

08/10/04 9.65 7.95 1.27 5.41 10.49

09/08/04 8.85 7.91 1.22 5.46 10.36

10/05/04 7.43 7.83 1.20 5.43 10.24

11/02/04 8.31 7.93 1.15 5.63 10.24

11/30/04 8.53 7.94 1.15 5.63 10.24

01/04/05 7.86 7.87 1.11 5.65 10.08

02/01/05 7.22 7.74 1.03 5.68 9.80

03/01/05 9.54 7.87 1.09 5.68 10.05

03/29/05 8.04 7.99 0.96 6.07 9.91

04/26/05 7.38 8.03 0.90 6.22 9.84

05/24/05 8.41 8.11 0.86 6.40 9.83

07/12/05 7.59 8.08 0.86 6.35 9.81

08/02/05 7.53 8.13 0.80 6.53 9.72

09/13/05 7.84 8.16 0.77 6.63 9.70

10/04/05 7.81 8.16 0.77 6.62 9.69

11/01/05 7.74 8.15 0.77 6.61 9.69

11/29/05 7.49 8.15 0.77 6.61 9.69

01/04/06 7.94 8.18 0.75 6.68 9.68

01/24/06 8.39 8.14 0.74 6.66 9.62

Means are of the last 20 SRT tests.

Lower Confidence Limit = mean - 2 * Std Dev CV = 9.09

Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 19%

Standard Reference Toxicant Data 

Cyprinella leedsi LC50

4

6

8

10

12

07/22/0408/10/0409/08/0410/05/0411/02/0411/30/0401/04/0502/01/0503/01/0503/29/0504/26/0505/24/0507/12/0508/02/0509/13/0510/04/0511/01/0511/29/0501/04/0601/24/06

Test Date

mg/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit
13

Appendix 3. (continued) 
14

Appendix 3. (continued) 
15

Appendix 3. (continued) 
16

Appendix 3. (continued) 
17

Appendix 3. (continued) 

Ceriodaphnia dubia Acute Standard Reference Toxicant Test 

START DATE: 01/31/06 END DATE: 02/04/06 DURATION: 96 H 

TOXICANT : NaCl 

 RAW DATA: Concentration Number Mortalities 

 --- ---- (ug/L) Exposed 

 0.00 20 0 

 1.50 20 0 

 2.00 20 11 

 2.50 20 20 

 3.00 20 20 

 4.00 20 20 

 SPEARMAN-KARBER TRIM: .00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 1.94* 

 95% LOWER CONFIDENCE: 1.84 

 95% UPPER CONFIDENCE: 2.06 

________________________________________________________________ 

Cyprinella leedsi Acute Standard Reference Toxicant Test 

 START DATE: 01/24/06 END DATE: 01/28/06 DURATION: 96 H 

 TOXICANT : NaCl 

 RAW DATA: Concentration Number Mortalities 

 (ug/L) Exposed 

 0.00 20 0 

 6.00 20 1 

 7.00 20 1 

 8.00 20 7 

 9.00 20 14 

 10.00 20 19 

 SPEARMAN-KARBER TRIM: 5.00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 8.39* 

 95% LOWER CONFIDENCE: 8.06 

 95% UPPER CONFIDENCE: 8.74 

*LC50 calculated at 48 hours to match environmental test duration 
18

Appendix 4. Test Organism Receipts 
19

Appendix 4. (continued) 
20

Appendix 5. Chemical Analyses performed on the effluent from the City of Homestead MunicipaOnce-Through Non-Contact Cooling Water Discharge Outfall D-001, sampled on February 13, 20

Date Sampled Field ID Analysis Component Result Units 2/13/2006 10:00 51-HPP-1 Bio-AGP/LimNut Algal Growth Potential 0.3 mg DryWt/L 2/13/2006 10:00 51-HPP-1 Bio-Chl-a Chlorophyll-A, Monochromatic, Water 0.85 ug/L 2/13/2006 10:00 51-HPP-1 Bio-Chl-a Phaeophytin-A, Monochromatic, Water 1.5 ug/L 2/13/2006 10:00 51-HPP-1 Bio-Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 LC50 2/13/2006 10:00 51-HPP-1 Bio-Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 100 LC50 2/13/2006 10:00 51-HPP-1 BNA-Water 1,2,4-Trichlorobenzene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 1,2-Dichlorobenzene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 1,3-Dichlorobenzene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 1,4-Dichlorobenzene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2,4,6-Trichlorophenol 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2,4-Dichlorophenol 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2,4-Dimethylphenol 48 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2,4-Dinitrophenol 14 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2,4-Dinitrotoluene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2,6-Dinitrotoluene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2-Chloronaphthalene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2-Chlorophenol 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2-Methyl-4,6-dinitrophenol 2.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 2-Nitrophenol 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 3,3'-Dichlorobenzidine 38 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 4,4'-DDD 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 4,4'-DDE 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 4,4'-DDT 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 4-Bromophenyl phenyl ether 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 4-Chloro-3-methylphenol 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 4-Chlorophenyl phenyl ether 1.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water 4-Nitrophenol 14 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Acenaphthene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Acenaphthylene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Aldrin 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water alpha-BHC 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Anthracene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Benzidine 96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Benzo(a)anthracene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Benzo(a)pyrene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Benzo(b)fluoranthene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Benzo(g,h,i)perylene 0.96 ug/L 
21

Appendix 5. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 2/13/2006 10:00 51-HPP-1 BNA-Water Benzo(k)fluoranthene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water beta-BHC 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-chloroethoxy)methane 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-chloroethyl)ether 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-chloroisopropyl)ether 2.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Bis(2-ethylhexyl)phthalate 14 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Butyl benzyl phthalate 4.8 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Chrysene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water delta-BHC 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Dibenzo(a,h)anthracene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Dieldrin 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Diethyl phthalate 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Dimethyl phthalate 48 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Di-n-butyl phthalate 4.8 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Di-n-octyl phthalate 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Endosulfan I 3.8 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Endosulfan II 3.8 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Endosulfan sulfate 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Endrin 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Endrin aldehyde 3.8 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Fluoranthene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Fluorene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water gamma-BHC 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Heptachlor 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Heptachlor epoxide 1.4 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Hexachlorobenzene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Hexachlorobutadiene 2.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Hexachlorocyclopentadiene 2.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Hexachloroethane 2.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Indeno(1,2,3-cd)pyrene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Isophorone 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Naphthalene 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Nitrobenzene 1.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water N-Nitrosodimethylamine 1.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water N-Nitrosodi-n-propylamine 1.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water N-Nitrosodiphenylamine 2.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Pentachlorophenol 2.9 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Phenanthrene 0.96 ug/L 
22

Appendix 5. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 2/13/2006 10:00 51-HPP-1 BNA-Water Phenol 0.96 ug/L 2/13/2006 10:00 51-HPP-1 BNA-Water Pyrene 0.96 ug/L 2/13/2006 10:15 BLANK BNA-Water 1,2,4-Trichlorobenzene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 1,2-Dichlorobenzene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 1,3-Dichlorobenzene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 1,4-Dichlorobenzene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 2,4,6-Trichlorophenol 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 2,4-Dichlorophenol 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 2,4-Dimethylphenol 49 ug/L 2/13/2006 10:15 BLANK BNA-Water 2,4-Dinitrophenol 15 ug/L 2/13/2006 10:15 BLANK BNA-Water 2,4-Dinitrotoluene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 2,6-Dinitrotoluene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 2-Chloronaphthalene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 2-Chlorophenol 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 2-Methyl-4,6-dinitrophenol 2.9 ug/L 2/13/2006 10:15 BLANK BNA-Water 2-Nitrophenol 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 3,3'-Dichlorobenzidine 39 ug/L 2/13/2006 10:15 BLANK BNA-Water 4,4'-DDD 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water 4,4'-DDE 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water 4,4'-DDT 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water 4-Bromophenyl phenyl ether 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 4-Chloro-3-methylphenol 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water 4-Chlorophenyl phenyl ether 1.9 ug/L 2/13/2006 10:15 BLANK BNA-Water 4-Nitrophenol 15 ug/L 2/13/2006 10:15 BLANK BNA-Water Acenaphthene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Acenaphthylene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Aldrin 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water alpha-BHC 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Anthracene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Benzidine 97 ug/L 2/13/2006 10:15 BLANK BNA-Water Benzo(a)anthracene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Benzo(a)pyrene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Benzo(b)fluoranthene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Benzo(g,h,i)perylene 0.97 ug/L 

2/13/2006 10:15 BLANK BNA-Water Benzo(k)fluoranthene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water beta-BHC 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Bis(2-chloroethoxy)methane 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Bis(2-chloroethyl)ether 0.97 ug/L 
23

Appendix 5. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 2/13/2006 10:15 BLANK BNA-Water Bis(2-chloroisopropyl)ether 2.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Bis(2-ethylhexyl)phthalate 15 ug/L 2/13/2006 10:15 BLANK BNA-Water Butyl benzyl phthalate 4.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Chrysene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water delta-BHC 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Dibenzo(a,h)anthracene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Dieldrin 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Diethyl phthalate 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Dimethyl phthalate 49 ug/L 2/13/2006 10:15 BLANK BNA-Water Di-n-butyl phthalate 4.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Di-n-octyl phthalate 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Endosulfan I 3.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Endosulfan II 3.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Endosulfan sulfate 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Endrin 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Endrin aldehyde 3.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Fluoranthene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Fluorene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water gamma-BHC 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Heptachlor 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Heptachlor epoxide 1.5 ug/L 2/13/2006 10:15 BLANK BNA-Water Hexachlorobenzene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Hexachlorobutadiene 2.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Hexachlorocyclopentadiene 2.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Hexachloroethane 2.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Indeno(1,2,3-cd)pyrene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Isophorone 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Naphthalene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Nitrobenzene 1.9 ug/L 2/13/2006 10:15 BLANK BNA-Water N-Nitrosodimethylamine 1.9 ug/L 2/13/2006 10:15 BLANK BNA-Water N-Nitrosodi-n-propylamine 1.9 ug/L 2/13/2006 10:15 BLANK BNA-Water N-Nitrosodiphenylamine 2.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Pentachlorophenol 2.9 ug/L 2/13/2006 10:15 BLANK BNA-Water Phenanthrene 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Phenol 0.97 ug/L 2/13/2006 10:15 BLANK BNA-Water Pyrene 0.97 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Alachlor 0.6 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Ametryn 0.05 ug/L 
24

Appendix 5. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 2/13/2006 10:00 51-HPP-1 GC-Water Atrazine 0.05 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Azinphos Methyl 0.2 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Bromacil 0.2 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Butylate 0.2 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Chlorpyrifos Ethyl 0.05 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Chlorpyrifos Methyl 0.1 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Diazinon 0.05 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Ethion 0.05 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Ethoprop 0.1 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Fenamiphos 0.2 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Fonofos 0.1 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Hexazinone 0.1 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Malathion 0.15 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Metalaxyl 0.25 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Metolachlor 0.5 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Metribuzin 0.1 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Mevinphos 0.2 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Naled 0.8 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Norflurazon 0.15 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Parathion Ethyl 0.15 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Parathion Methyl 0.1 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Phorate 0.05 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Prometryn 0.15 ug/L 2/13/2006 10:00 51-HPP-1 GC-Water Simazine 0.05 ug/L 2/13/2006 10:15 BLANK GC-Water Alachlor 0.58 ug/L 2/13/2006 10:15 BLANK GC-Water Ametryn 0.048 ug/L 2/13/2006 10:15 BLANK GC-Water Atrazine 0.048 ug/L 2/13/2006 10:15 BLANK GC-Water Azinphos Methyl 0.19 ug/L 2/13/2006 10:15 BLANK GC-Water Bromacil 0.19 ug/L 2/13/2006 10:15 BLANK GC-Water Butylate 0.19 ug/L 2/13/2006 10:15 BLANK GC-Water Chlorpyrifos Ethyl 0.048 ug/L 2/13/2006 10:15 BLANK GC-Water Chlorpyrifos Methyl 0.096 ug/L 2/13/2006 10:15 BLANK GC-Water Diazinon 0.048 ug/L 2/13/2006 10:15 BLANK GC-Water Ethion 0.048 ug/L 2/13/2006 10:15 BLANK GC-Water Ethoprop 0.096 ug/L 2/13/2006 10:15 BLANK GC-Water Fenamiphos 0.19 ug/L 2/13/2006 10:15 BLANK GC-Water Fonofos 0.096 ug/L 2/13/2006 10:15 BLANK GC-Water Hexazinone 0.096 ug/L 
25

Appendix 5. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 2/13/2006 10:15 BLANK GC-Water Malathion 0.14 ug/L 2/13/2006 10:15 BLANK GC-Water Metalaxyl 0.24 ug/L 2/13/2006 10:15 BLANK GC-Water Metolachlor 0.48 ug/L 2/13/2006 10:15 BLANK GC-Water Metribuzin 0.096 ug/L 2/13/2006 10:15 BLANK GC-Water Mevinphos 0.19 ug/L 2/13/2006 10:15 BLANK GC-Water Naled 0.77 ug/L 2/13/2006 10:15 BLANK GC-Water Norflurazon 0.14 ug/L 2/13/2006 10:15 BLANK GC-Water Parathion Ethyl 0.14 ug/L 2/13/2006 10:15 BLANK GC-Water Parathion Methyl 0.096 ug/L 2/13/2006 10:15 BLANK GC-Water Phorate 0.048 ug/L 2/13/2006 10:15 BLANK GC-Water Prometryn 0.14 ug/L 2/13/2006 10:15 BLANK GC-Water Simazine 0.048 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Aluminum 5 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Arsenic 4 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Cadmium 0.05 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Calcium 86.5 mg/L 2/13/2006 10:00 51-HPP-1 Metals-Water Chromium 2 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Copper 13.1 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Iron 10 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Lead 0.075 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Magnesium 5.8 mg/L 2/13/2006 10:00 51-HPP-1 Metals-Water Nickel 1.9 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Selenium 0.5 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Silver 0.025 ug/L 2/13/2006 10:00 51-HPP-1 Metals-Water Zinc 14 ug/L 2/13/2006 10:15 BLANK Metals-Water Aluminum 5 ug/L 2/13/2006 10:15 BLANK Metals-Water Arsenic 4 ug/L 2/13/2006 10:15 BLANK Metals-Water Cadmium 0.05 ug/L 2/13/2006 10:15 BLANK Metals-Water Calcium 0.05 mg/L 2/13/2006 10:15 BLANK Metals-Water Chromium 2 ug/L 2/13/2006 10:15 BLANK Metals-Water Copper 0.25 ug/L 2/13/2006 10:15 BLANK Metals-Water Iron 10 ug/L 2/13/2006 10:15 BLANK Metals-Water Lead 0.075 ug/L 2/13/2006 10:15 BLANK Metals-Water Magnesium 0.01 mg/L 2/13/2006 10:15 BLANK Metals-Water Nickel 1 ug/L 2/13/2006 10:15 BLANK Metals-Water Selenium 0.5 ug/L 2/13/2006 10:15 BLANK Metals-Water Silver 0.025 ug/L 2/13/2006 10:15 BLANK Metals-Water Zinc 3 ug/L 
26

Appendix 5. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Component Result Units 2/13/2006 10:00 51-HPP-1 Nutrients-Liquid Alkalinity 214 mg CaCO3/L 2/13/2006 10:00 51-HPP-1 Nutrients-Liquid Ammonia-N 0.01 mg N/L 2/13/2006 10:00 51-HPP-1 Nutrients-Liquid Kjeldahl Nitrogen 0.24 mg N/L 2/13/2006 10:00 51-HPP-1 Nutrients-Liquid NO2NO3-N 2.3 mg N/L 2/13/2006 10:00 51-HPP-1 Nutrients-Liquid O-Phosphate-P 0.006 mg P/L 2/13/2006 10:00 51-HPP-1 Nutrients-Liquid Total-P 0.02 mg P/L 2/13/2006 10:15 BLANK Nutrients-Liquid Ammonia-N 0.01 mg N/L 2/13/2006 10:15 BLANK Nutrients-Liquid Kjeldahl Nitrogen 0.08 mg N/L 2/13/2006 10:15 BLANK Nutrients-Liquid NO2NO3-N 0.005 mg N/L 2/13/2006 10:15 BLANK Nutrients-Liquid O-Phosphate-P 0.004 mg P/L 2/13/2006 10:15 BLANK Nutrients-Liquid Total-P 0.02 mg P/L 2/13/2006 10:00 51-HPP-1 Overflow Oil and Grease 1.7 mg/L 2/13/2006 10:15 BLANK Overflow Oil and Grease 1.7 mg/L 
27

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 7 2 1 11 12 0 6 0 2 1 3 18 B 19 S 20 1

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 7 2 1 11 12 0 6 0 2 1 3 18 X 19 S 20 1

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 2 7 2 1 11 12 0 6 0 2 1 3 18 S 19 S 20 1

66

Biological Analyses of City of Homestead Municipal Power Plant 

Once-Through Non-Contact Cooling Water Discharge effluent 

sampled on February 13, 2006. NPDES #FL0002721.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of City of Homestead Municipal Power Plant OnceThrough Non-Contact Cooling Water Discharge effluent sampled 

on February 13, 2006. NPDES #FL0002721.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of City of Homestead Municipal 

Power Plant Once-Through Non-Contact Cooling Water Discharge 

effluent sampled on February 13, 2006. NPDES #FL0002721.