# Patent

## Title
Microbial delivery system

## Abstract

The present invention provides methods and compositions for treating or preventing allergic responses, particularly anaphylactic allergic responses, in subjects who are allergic to allergens or susceptible to allergies. Methods of the present invention utilize administration of microorganisms to subjects, where the microorganisms produce allergens and protect the subjects from exposure to the allergens until phagocytosed by antigen-presenting cells. Particularly preferred microorganisms are gram-negative bacteria, gram-positive bacteria, and yeast. Particularly preferred allergens are proteins found in foods, venoms, drugs and latex that elicit allergic reactions and anaphylactic allergic reactions in individuals who are allergic to the proteins or are susceptible to allergies to the proteins. The proteins may also be modified to reduce the ability of the proteins to bind and crosslink IgE antibodies and thereby reduce the risk of eliciting anaphylaxis without affecting T-cell mediated Th1-type immunity.

## Background
PRIORITY INFORMATION
The present application claims priority under 35 U.S.C. 119(e) to the U.S. Provisional Patent Application Ser. No. 60/195,035 entitled “Bacterial Polypeptide Delivery” filed Apr. 6, 2000.


SEQUENCE LISTING
In accordance with 37 C.F.R. §1.52(e)(5), a Sequence Listing in the form of a text file (entitled “Sequence Listing,” created on Feb. 24, 2011 and 36 kilobytes) is incorporated herein by reference in its entirety.
RELATED APPLICATIONS
The present invention is generally in the area of controlled delivery of antigens for use in vaccination or induction of tolerance to allergens, and in particular relates to cellular delivery of proteins and polypeptides. This application is related to U.S. Ser. No. 60/169,330 entitled “Controlled Delivery of Antigens” filed Dec. 6, 1999; U.S. Ser. No. 09/141,220 entitled “Methods and Reagents for Decreasing Clinical Reaction to Allergy” filed Aug. 27, 1998; U.S. Ser. No. 09/455,294 entitled “Peptide Antigens” filed Dec. 6, 1999; U.S. Ser. No. 09/494,096 filed Jan. 28, 2000 entitled “Methods and Reagents for Decreasing Clinical Reaction to Allergy” by Bannon et al.; and U.S. Ser. No. 09/527,083 entitled “Immunostimulatory Nucleic Acids and Antigens” by Caplan filed Mar. 16, 2000; the teachings of which are all incorporated herein by reference in their entirety.
BACKGROUND OF THE INVENTION
Allergic reactions pose serious public health problems worldwide. Pollen allergy alone (allergic rhinitis or hay fever) affects about 10-15% of the population, and generates huge economic costs. For example, reports estimate that pollen allergy generated $1.8 billion of direct and indirect expenses in the United States in 1990 (Fact Sheet, National Institute of Allergy and Infectious Diseases; McMenamin, Annals of Allergy 73:35, 1994). Asthma, which can be triggered by exposure to antigens, is also a serious public health problem, and like anaphylactic allergic reactions, can lead to death in extreme cases. Asthma currently accounts for millions of visits yearly to hospitals and is increasing in frequency. The only treatment currently available is for alleviation of symptoms, for example, to relieve constriction of airways. More serious than the economic costs associated with pollen and other inhaled allergens (e.g., molds, dust mites, animal danders) is the risk of an anaphylactic allergic reaction observed with allergens such as food allergens, insect venoms, drugs, and latex.
Allergic reactions result when an individual's immune system overreacts, or reacts inappropriately, to an encountered antigen. Typically, there is no allergic reaction the first time an individual is exposed to a particular antigen. However, it is the initial response to an antigen that primes the system for subsequent allergic reactions. In particular, the antigen is taken up by antigen presenting cells (APC; e.g., macrophages and dendritic cells) that degrade the antigen and then display antigen fragments to T cells. T cells, in particular CD4+ “helper” T-cells, respond by secreting a collection of cytokines that have effects on other immune system cells. The profile of cytokines secreted by responding CD4+ T cells determines whether subsequent exposures to the antigen will induce allergic reactions. Two classes of CD4+ T cells (Th1 and Th2) influence the type of immune response that is mounted against an antigen.
Th2 cells can secrete a variety of cytokines and interleukins including IL-4, IL-5, IL-6, IL-10 and IL-13. One effect of IL-4 is to stimulate the maturation of B cells that produce IgE antibodies specific for the antigen. Allergic responses to allergens are characterized by the production of antigen-specific IgE antibodies which are dependent on help from IL-4 secreting CD4+ T cells. These antigen-specific IgE antibodies attach to receptors on the surface of mast cells, basophils and eosinophils, where they act as a trigger to initiate a rapid allergic reaction upon the next exposure to antigen. When the individual encounters the antigen a second time, the antigen is quickly bound by these surface-associated IgE molecules. Each antigen typically has more than one IgE binding site, so that the surface-bound IgE molecules quickly become crosslinked to one another through their simultaneous (direct or indirect) associations with antigen. Such cross-linking induces mast cell degranulation, resulting in the release of histamines and other substances that trigger allergic reactions. Individuals with high levels of IgE antibodies are known to be particularly prone to allergies.
Current treatments for allergies involve attempts to “vaccinate” a sensitive individual against a particular allergen by periodically injecting or treating the individual with a crude suspension of the raw allergen. The goal, through controlled administration of known amounts of antigen, is to modulate the IgE response mounted in the individual. If the therapy is successful, the individual's IgE response is diminished, or can even disappear. However, the therapy requires several rounds of vaccination, over an extended time period (3-5 years), and very often does not produce the desired results. Moreover, certain individuals suffer anaphylactic reactions to the vaccines, despite their intentional, controlled administration.
Clearly, there is a need for treatments and preventive methods for patients with allergies to allergens that elicit serious allergic responses including anaphylaxis.
SUMMARY OF THE INVENTION
The present invention provides methods and compositions for modulating the immune response in a subject. It is an aspect of the present invention to provide a method of treating or preventing undesirable allergic reactions and anaphylactic allergic reactions to allergens in a subject. Methods of the present invention involve administering to subjects, microorganisms that express or produce allergens of interest. Without being limited to the proposed mechanism of action, after administration the microorganisms are taken up by antigen-presenting cells in the subject where the expressed antigens are released. After being processed inside the antigen-presenting cells and displayed on the cell surface, the processed antigens activate T-cell mediated immune responses. Use of genetically modified microorganisms to express and deliver allergens to a subject therefore reduces the exposure of the allergens to the subject's IgE antibodies, which lead to allergic reactions and possibly anaphylaxis. The present invention therefore reduces the risk of anaphylaxis during immunotherapy. Furthermore, the microorganisms may act as a natural adjuvant to enhance desirable Th1-type immune responses.
In a preferred embodiment, microorganisms are genetically modified to express selected polypeptides or proteins, and are used as delivery vehicles in accordance with the present invention. Such microorganisms include but are not limited to bacteria, viruses, fungi (including yeast), algae, and protozoa. Generally, preferred microorganisms for use in accordance with the present invention are single cell, single spore or single virion organisms. Additionally, included within the scope of the present invention are cells from multi-cellular organisms which have been modified to produce a polypeptide of interest.
In a particularly preferred embodiment, bacteria or yeast are used as microorganisms to express and deliver allergenic proteins to individuals to treat or prevent allergic responses, including anaphylactic allergic responses, to the allergens. Gram-positive and gram-negative bacteria may be used in the present invention has delivery vehicles. Antigens expressed by the bacteria may be secreted or non-secreted. Secretion of proteins may involve secretion into the cellular medium. For gram-negative bacteria and yeast, secretion may involve secretion into the periplasm. Secretion of polypeptides may be facilitated by secretion signal peptides. In certain preferred embodiments microorganisms expressing allergenic compounds may be administered to subjects in compositions as attenuated microorganisms, non-pathogenic microorganisms, non-infectious microorganisms, or as killed microorganisms. Preferably, the killed microorganisms are killed without degrading the antigenic properties of the polypeptides.
In another preferred embodiment, the allergens utilized are allergens found in foods, venom, drugs and a rubber-based products. Particularly preferred protein allergens are found in foods and venoms that elicit anaphylactic allergic responses in subjects who are allergic to the allergens. Included in the present invention are peptides and polypeptides whose amino acid sequences are found in the proteins allergens in nature. Also included in the present invention are allergens that have modifications that reduce the ability of the peptides, polypeptides and proteins to bind and crosslink IgE antibodies. Also included in the present invention are non-peptide allergens that are produced by microorganisms and include for example antibiotics such as penicillin.
In another aspect of the invention, compositions for use in treating or prevent allergic and anaphylactic allergic responses in a subject comprise microorganisms that have been engineered by the hand of man, and preferably by the introduction of one or more introduced nucleic acids, to produce allergens in accordance with the present invention. In certain preferred embodiments, the produced allergens are peptides, polypeptides, or proteins encoded by the introduced nucleic acids(s).



BRIEF DESCRIPTION OF FIGURES
FIG. 1. Experiments designed to determine the optimal temperature for heat-killing bacteria (E. coli) are depicted in graphic form. The number of surviving colonies in aliquots of samples are shown as a function of temperature (Celsius).
FIG. 2. Determination of protein produce per cell. The optical density (O.D.) of the HIS-tagged Ara h 2 allergen was determined from an immunoblot where different concentrations of E. coli extract has been electrophoresed on SDS-PAGE gels. The allergen O.D. was used to estimate the amount of protein produced by that extract.
FIG. 3. Results of ELISA analysis of Ara h 2-specific IgG antibodies produced in mice following injection of E. coli producing Ara h 2. IgG1 is on the left and IgG2a is on the right.
FIG. 4. Results of ELISA analysis of Ara h 3-specific IgG antibodies produced in mice following injection of E. coli producing Ara h 3. IgG1 is on the left and IgG2a is on the right.
FIG. 5 shows an example of how IgE binding epitopes were mapped to a specific amino acid sequence on the Ara h 1 allergen. In particular, FIG. 5 depicts twenty-two 10-mer peptides (SEQ ID NOs. 45-66) that span amino acid residues 82-133 (SEQ ID NO. 44) of the Ara h 1 allergen (SEQ ID NO. 2). This region of the Ara h 1 allergen includes epitopes 4, 5, 6, and 7, as identified in Table 1.
FIG. 6 shows an example of how IgE binding epitopes were mapped to a specific amino acid sequence on the Ara h 2 allergen. In particular, FIG. 6 depicts seven 10-mer peptides (SEQ ID NOs. 68-74) that span amino acid residues 55-76 (SEQ ID NO. 67) of the Ara h 2 allergen (SEQ ID NO. 4). This region of the Ara h 2 allergen includes epitopes 6 and 7 as identified in Table 2.
FIG. 7 shows an example of how IgE binding epitopes were mapped to a specific amino acid sequence on the Ara h 3 allergen. In particular, FIG. 7 depicts six 15-mer peptides (SEQ ID NOs. 76-81) that span amino acid residues 299-321 (SEQ ID NO. 75) of the Ara h 3 allergen (SEQ ID NO. 6). This region of the Ara h 3 allergen includes epitope 4 as identified in Table 3.
FIG. 8 shows the effect the modified Ara h 2 protein has on IgE binding.
FIG. 9 shows the results of T cell proliferation assays using the wild-type and modified Ara h 2 protein.



DEFINITIONS
“Allergen”: An “allergen” is an antigen that (i) elicits an IgE response in an individual; and/or (ii) elicits an asthmatic reaction (e.g., chronic airway inflammation characterized by eosinophilia, airway hyperresponsiveness, and excess mucus production), whether or not such a reaction includes a detectable IgE response). Preferred allergens for the purpose of the present invention are peptide, polypeptide and protein allergens. An exemplary list of protein allergens is presented as an Appendix. This list was adapted from ftp://biobase.dk/pub/who-iuis/allergen.list (updated on Mar. 1, 2000), which provides lists of known allergens. Other preferred allergens are chemical compounds such as small molecules that are produced by proteins. In some embodiments, an allergen is a subset of antigens which elicits IgE production in addition to other isotypes of antibodies.
“Allergic reaction”: An allergic reaction is a clinical response by an individual to an antigen. Symptoms of allergic reactions can affect cutaneous (e.g., urticaria, angioedema, pruritus), respiratory (e.g., wheezing, coughing, laryngeal edema, rhinorrhea, watery/itching eyes) gastrointestinal (e.g., vomiting, abdominal pain, diarrhea), and/or cardiovascular (if a systemic reaction occurs) systems. For the purposes of the present invention, an asthmatic reaction is considered to be a form of allergic reaction. A decreased allergic reaction is characterized by a decrease in clinical symptoms following treatment of symptoms associated with exposure to an allergen, which can involve respiratory, gastrointestinal, skin, eyes, ears and mucosal surfaces in general.
“Anaphylactic antigen”: An “anaphylactic antigen” according to the present invention is an antigen (or allergen) that is recognized to present a risk of anaphylactic reaction in allergic individuals when encountered in its natural state, under natural conditions. For example, for the purposes of the present invention, pollens and animal danders or excretions (e.g., saliva, urine) are not considered to be anaphylactic antigens. On the other hand, food antigens, insect antigens, drugs, and rubber (e.g., latex) antigens latex are generally considered to be anaphylactic antigens. Food antigens are particularly preferred anaphylactic antigens for use in the practice of the present invention. Particularly interesting anaphylactic antigens are those (e.g., nuts, seeds, and fish) to which reactions are commonly so severe as to create a risk of death.
“Anaphylaxis” or “anaphylactic reaction”, as used herein, refers to an immune response characterized by mast cell degranulation secondary to antigen-induced cross-linking of the high-affinity IgE receptor on mast cells and basophils with subsequent mediator release and the production of pathological responses in target organs, e.g., airway, skin digestive tract and cardiovascular system. As is known in the art, the severity of an anaphylactic reaction may be monitored, for example, by assaying cutaneous reactions, puffiness around the eyes and mouth, and/or diarrhea, followed by respiratory reactions such as wheezing and labored respiration. The most severe anaphylactic reactions can result in loss of consciousness and/or death.
“Antigen”: An “antigen” is (i) any compound or composition that elicits an immune response; and/or (ii) any compound that binds to a T cell receptor (e.g., when presented by an MHC molecule) or to an antibody produced by a B-cell. Those of ordinary skill in the art will appreciate that an antigen may be collection of different chemical compounds (e.g., a crude extract or preparation) or a single compound (e.g., a protein). Preferred antigens are peptide, polypeptide or protein antigens. In some embodiments, an antigen is a molecule that elicits production of antibody (a humoral response) or an antigen-specific reaction with T cells (a cellular response).
“Antigen presenting cells”: “Antigen presenting cells” or APCs” include known APCs such as Langerhans cells, veiled cells of afferent lymphatics, dendritic cells and interdigitating cells of lymphoid organs. The term also includes mononuclear cells such as lymphocytes and macrophages which take up polypeptides and proteins according to the invention. In some embodiments, an antigen presenting cell (an APC) is a cell which processes and presents peptides to T cells to elicit an antigen-specific response.
“Attenuation”: “Attenuation” of microorganisms as used herein refers to the manipulation of the microorganisms so that the microorganisms do not induce significant toxic reactions in individuals or laboratory test animals. The manipulations include genetic methods and are well known in the art.
An epitope is a binding site including an amino acid motif of between approximately six and fifteen amino acids which can be bound by either an immunoglobulin or recognized by a T cell receptor when presented by an antigen presenting cell in conjunction with the major histocompatibility complex (MHC). A linear epitope is one where the amino acids are recognized in the context of a simple linear sequence. A conformational epitope is one where the amino acids are recognized in the context of a particular three dimensional structure. An immunodominant epitope is one which is bound by antibody in a large percentage of the sensitized population or where the titer of the antibody is high, relative to the percentage or titer of antibody reaction to other epitopes present in the same protein.
“IgE binding site”: An IgE binding site is a region of an antigen that is recognized by an anti-antigen IgE molecule. Such a region is necessary and/or sufficient to result in (i) binding of the antigen to IgE; (ii) cross-linking of anti-antigen IgE; (iii) degranulation of mast cells containing surface-bound anti-antigen IgE; and/or (iv) development of allergic symptoms (e.g., histamine release). In general, IgE binding sites are defined for a particular antigen or antigen fragment by exposing that antigen or fragment to serum from allergic individuals (preferably of the species to whom inventive compositions are to be administered). It will be recognized that different individuals may generate IgE that recognize different epitopes on the same antigen. Thus, it is typically desirable to expose antigen or fragment to a representative pool of serum samples. For example, where it is desired that sites recognized by human IgE be identified in a given antigen or fragment, serum is preferably pooled from at least 5-10, preferably at least 15, individuals with demonstrated allergy to the antigen. Those of ordinary skill in the art will be well aware of useful pooling strategy in other contexts.
“Immunologic inducing agents”: The term “immunological inducing agents” is used herein as agents that prompt the expression of Th1 stimulating cytokines by T-cells and include factors such as, CD40, CD40 ligand, oligonucleotides containing CpG motifs, TNF, and microbial extracts such as preparations of Staphylococcus aureus, heat killed Listeria, and modified cholera toxin.
Immunostimulatory sequences are oligodeoxynucleotides of bacterial, viral or invertebrate origin that are taken-up by APCs and activate them to express certain membrane receptors (e.g., B7-1 and B7-2) and secrete various cytokines (e.g., IL-1, IL-6, IL-12, TNF). These oligodeoxynucleotides containing unmethylated CpG motifs cause brisk activation and when injected into animals in conjunction with antigen, appear to skew the immune response to a Th1-type response. See, for example, Yamamoto, et al., Microbiol. Immunol. 36, 983 (1992); Krieg, et al., Nature 374, 546-548 (1995); Pisetsky, Immunity 5, 303 (1996); and Zimmerman, et al., J. Immunol. 160, 3627-3630 (1998).
“Inducible promoter”: The term “inducible promoter”, as used herein, means a promoter site which is activated directly by the presence or absence of a chemical agent or indirectly by an environmental stimulus such as temperature changes. A promoter is the region of DNA at which the enzyme RNA polymerase binds and initiates the process of gene transcription.
“Mast cell”: As will be apparent from context, the term “mast cell” is often used herein to refer to one or more of mast cells, basophils, and other cells having IgE receptors, which when activated by crosslinking bound IgE molecules, releases histamines, vasodilators, and/or other mediators of allergic responses.
“Microorganisms”: “Microorganisms” as used herein are cells, bacteria, fungi, viruses, algae, and protozoa. Preferred microorganisms can be genetically manipulated to produce a desired polypeptide(s).
“Peptide”: According to the present invention, a “peptide” comprises a string of at least three amino acids linked together by peptide bonds. Inventive peptides preferably contain only natural amino acids, although non-natural amino acids (i.e., compounds that do not occur in nature but that can be incorporated into a polypeptide chain; see, for example, http://www.cco.caltech.edu/ ̃dadgrp/Unnatstruct.gif, which displays structures of non-natural amino acids that have been successfully incorporated into functional ion channels) and/or amino acid analogs as are known in the art may alternatively be employed. Also, one or more of the amino acids in an inventive peptide may be modified, for example, by the addition of a chemical entity such as a carbohydrate group, a phosphate group, a farnesyl group, an isofarnesyl group, a fatty acid group, a linker for conjugation, functionalization, or other modification, etc.
A peptide or polypeptide is derived from a protein if the amino acid sequence of the peptide or polypeptide is found within the amino acid sequence of the protein. The sequences are preferably identical but may have a sequence homology between approximately 80-100%. It is also recognized that amino acid residues may be replaced with other amino acids residues with similar physical properties such as hydrophobicity, hydrophilicity, charge, aromatic structures and polarity.
“Reduced IgE binding”: An inventive composition or antigen is considered to have “reduced IgE binding” if it demonstrates a lower level of interaction with IgE when compared with unmodified antigen in any available assay. For example, a modified antigen is considered to have reduced IgE binding if (i) its affinity for anti-antigen IgE (assayed, for example, using direct binding studies or indirect competition studies) is reduced at least about 2-5 fold, preferably at least about 10, 20, 50, or 100 fold as compared with intact antigen; (ii) ability of the modified antigen to support cross-linking of anti-antigen IgE is reduced at least about 2-fold, preferably at least about 5, 10, 20, 50, or 100 fold as compared with intact antigen; (iii) mast cells containing surface-bound anti-antigen IgE degranulate less (at least about 2 fold, preferably at least about 3, 5, 10, 20, 50, or 100 fold less) when contacted with modified as compared with unmodified antigen; and/or (iv) individuals contacted with modified antigen develop fewer (at least about 2 fold, preferably at least about 3, 5, 10, 20, 50, or 100 fold fewer) allergic symptoms, or developed symptoms are reduced in intensity when exposed to modified antigens as compared with unmodified antigens.
“Secretion signals”: A secretion signal is any amino acid sequence which when conjugated to a peptide, polypeptide or protein facilitates the transport of the conjugate fusion proteins across cell membranes. For uses of secretion signals in microorganisms, transport of fusion proteins involves crossing an inner membrane into the periplasm. It is preferred that secretion signals also facilitate transport of fusion proteins across an outer membrane into an extracellular medium. Secretion of proteins into the extracellular medium is considered “excretion”.
“Sensitized mast cell”: A “sensitized” mast cell is a mast cell that has surface-bound antigen specific IgE molecules. The term is necessarily antigen specific. That is, at any given time, a particular mast cell will be “sensitized” to certain antigens (those that are recognized by the IgE on its surface) but will not be sensitized to other antigens.
“Small molecules”: As used herein, the term “small molecule” refers to a compound either synthesized in the laboratory or found in nature. Typically, a small molecule is organic and is characterized in that it contains several carbon-carbon bonds, and has a molecular weight of less than 1500 daltons, although this characterization is not intended to be limiting for the purposes of the present invention. Examples of “small molecules” that are allergens include without limitation penicillin, alcohols, and aspirin. Non-organic small molecule allergens include sulfites present in wine, for example.
“Susceptible individual”: According to the present invention, a person is susceptible to an allergic reaction if (i) that person has ever displayed symptoms of allergy after exposure to a given antigen; (ii) members of that person's genetic family have displayed symptoms of allergy against the allergen, particularly if the allergy is known to have a genetic component; and/or (iii) antigen-specific IgE are found in the individual, whether in serum or on mast cells.
“Th1 response” and “Th2 response”: Certain preferred peptides, polypeptides, proteins and compositions of the present invention are characterized by their ability to suppress a Th2 response and/or to stimulate a Th1 response preferentially as compared with their ability to stimulate a Th2 response. Th1 and Th2 responses are well-established alternative immune system responses that are characterized by the production of different collections of cytokines and/or cofactors. For example, Th1 responses are generally associated with production of cytokines such as IL-1β, IL-2, IL-12, IL-18, IFNα, IFNγ, TNF13, etc; Th2 responses are generally associated with the production of cytokines such as IL-4, IL-5, IL-10, etc. The extent of T cell subset suppression or stimulation may be determined by any available means including, for example, intra-cytoplasmic cytokine determination. In preferred embodiments of the invention, Th2 suppression is assayed, for example, by quantitation of IL-4, IL-5, and/or IL-13 in stimulated T cell culture supernatant or assessment of T cell intra-cytoplasmic (e.g., by protein staining or analysis of mRNA) IL-4, IL-5, and/or IL-13; Th1 stimulation is assayed, for example, by quantitation of IFNα, IFNγ, IL-2, IL-12, and/or IL-18 in activated T cell culture supernatant or assessment of intra-cytoplasmic levels of these cytokines.
DESCRIPTION OF CERTAIN PREFERRED EMBODIMENTS
The present invention provides compositions and methods for modulating the immune response in a subject. It is an aspect of the present invention that undesirable allergic immune responses to antigens in a subject are treated or prevented by administering modified cells, virions, or spores (“microorganisms”) that express allergens of interest. By using genetically modified microorganisms to express and deliver allergens, exposure of the allergens to the subject's IgE-mediated allergic immune response is reduced or eliminated. Without limitation to the mechanisms proposed, it is expected that the modified microorganisms of the present invention are engulfed by antigen-presenting cells (APCs) such as macrophages and dendritic cells without exposing allergens to IgE antibodies. Once inside the APCs, the expressed allergens are released by lysis of the microorganisms or secretion of the antigen by the microorganisms. The allergens are then processed, for example through partial digestion by the APCs, and displayed on the cell surface.
Once the processed antigens are displayed on the cell surface, activation of the cytotoxic T cell response and helper T cell response promotes cellular immune response and Th1-mediated B cell response to protein allergens. In addition, the processed antigens have a reduced ability (or no ability) to bind and crosslink IgE antibodies located on the surface of mast cells and basophils leading to the release of histamines and other vasodilators responsible for allergic and sometimes fatal anaphylactic responses.
Host Microorganisms
Any microorganism capable of expressing (e.g., by expression of polypeptide or protein allergens, or by expression of polypeptide or protein enzymes involved in synthesis of small molecule allergens) allergens may be used as delivery vehicles in accordance with the present invention. Such microorganisms include but are not limited to bacteria, viruses, fungi (including yeast), algae, and protozoa. Generally, microorganisms are single cell, single spore or single virion organisms. Additionally, included within the scope of the present invention are cells from multi-cellular organisms which have been modified to produce a polypeptide of interest. Microorganisms that can be genetically manipulated to produce a desired polypeptide are preferred. (Ausubel et al. Current Protocols in Molecular Biology. Wiley and Sons, Inc. 1999, incorporated herein by reference) Genetic manipulation includes mutation of the host genome, insertion of genetic material into the host genome, deletion of genetic material of the host genome, transformation of the host with extrachromosomal genetic material, transformation with linear plasmids, transformation with circular plasmids, insertion of genetic material into the host (e.g., injection of mRNA), insertion of transposons, and chemical modification of genetic material. Methods for constructing nucleic acids (including an expressible gene), and introducing such nucleic acids into an expression system to express the encoded protein are well established in the art (see, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989, incorporated herein by reference).
Use of microorganisms such as bacteria and yeast for allergen delivery in accordance with the present invention offers many advantages over delivery of allergens that are not encapsulated inside microorganisms for immunotherapy. Generally, microorganisms, such as bacteria, are known to act as an adjuvant (for a review, see for example, Freytag et al. Curr Top Microbiol Immunol 236:215-36, 1999). Therefore, use of microorganisms to delivery allergens to subjects, and APCs of subjects, provides protection of the allergen from IgE-mediated allergic responses and also provides an adjuvant effect which elicits a Th1-type immune response from an individual susceptible to allergic responses. In addition, use of non-pathogenic, non-infectious, attenuated and/or killed microorganisms reduces or eliminates toxicity which may be associated with allergen delivery vehicles.
In a preferred embodiment, bacteria are used as protein delivery microorganisms. Generally, bacteria are classified as gram-negative or gram-positive depending on the structure of the cell walls. Those skilled in the art are capable of identifying gram-negative and gram-positive bacteria which may be used to express proteins in accordance with the present invention. Non-limiting examples of genera and species of gram-negative bacteria include Escherichia coli, Vibro cholera, Salmonella, Listeria, Legionella, Shigella, Yersenia, Citrobacter, Enterobacter, Klebsiella, Morganella, Proteus, Providencia, Serratia, Plesiomonas, Aeromonas. Non-limiting examples of genera and species of gram-positive bacteria which may be used in the present invention include Bacillus subtilis, Sporolactobacillus, Clostridium, Arthrobacter, Micrococcus, Mycobacterium, Peptococcus, Peptostreptococcus, and Lactococcus. 
Gram-negative bacterial systems for use as delivery vehicles are known and may be used in the present invention. For example, E. coli is a well-studied bacteria, and methods of protein expression in E. coli are well-established. Most strains of E. coli have the advantage of being non-pathogenic since E. coli is found naturally in the gut. Therefore, E. coli is preferred as a delivery vehicle in the present invention. In addition, Calderwood et al. (U.S. Pat. No. 5,747,028) utilize Vibrio cholerae as a delivery vehicle for production of antigens for use as a live vaccine against infectious organisms. Miller and Mekalanos (U.S. Pat. No. 5,731,196) utilize Salmonella as delivery vehicle for production of antigens for use as a live vaccine against infectious organisms. Hess et al. (Proc. Natl. Acad. Sci. USA 93:1458-1463, 1996) utilize recombinant attenuated Salmonella which secretes antigenic determinants of Listeria as a live vaccine to protect against listeriosis. Donner et al. (WO 98/50067) utilize attenuated Salmonella typhimurium as a gram-negative host for secretion of polypeptides for controlling fertility and also teach that other attenuated gram-negative strains including Yersinia may be used to express and secrete such polypeptides.
Gram-positive bacteria have also been studied as delivery vehicles for proteins to modulate an immune response in a subject. WO 97/14806 describes the use of Lactococcus to deliver polypeptides into a body to enhance the immune response to the polypeptides. However, WO 97/14806 does not teach the use of Lactococcus to treat patients with food allergies and venom allergies which may result in anaphylaxis
In another preferred embodiment, yeast are used as protein delivery microorganisms. It is well known that yeast are amenable to genetic manipulation to express a protein or proteins of choice (Ausubel et al. supra). Furthermore, in general most yeast are non-pathogenic. Without limitation to these species, two well-characterized species of yeast are the budding yeast Saccharomyces cerevisiae, and the fission yeast, Schizosaccharomyces pombe. Moreover, the administration of yeast that express protein antigens to alter an immune response has been studied. Duke et al. (U.S. Pat. No. 5,830,463; “Duke”) describe the use of yeast to express proteins after administration of the yeast to a mammal. However, Duke does not teach the use of yeast to treat patients with food allergies and venom allergies which may result in anaphylaxis.
Microorganisms of the present invention may be administered to a subject as live or dead microorganisms. Preferably if the microorganisms are administered as live microorganisms, they are non-pathogenic or attenuated pathogenic microorganisms. For applications of the invention where live microorganisms are administered to individuals, preferably the microorganisms are attenuated and/or are administered in suitable encapsulation materials and/or as pharmaceutical compositions as vaccines to decrease an individuals immune response to the microorganism and/or allergenic compounds. Generally, attenuation involves genetically modifying the infectious pathogenic microorganism to reduce or eliminate the infectious ability of the microorganism. Preferably, the microorganism is attenuated such that an individual inoculated with the microorganism does not suffer any cytotoxic effects from the presence of the microorganism. Particularly preferred attenuated microorganisms are infectious intracellular pathogens which are phagocytosed by antigen-presenting cells in individuals who are exposed to the microorganism. Examples of microorganisms which are intracellular pathogens include Salmonella, Mycobacterium, Leishmania, Legionella, Listeria, and Shigella. 
Microorganisms of the present invention may be administered to subjects after killing the microorganisms. Any method of killing the microorganisms may be utilized that does not greatly alter the antigenicity of the expressed polypeptides. Methods of killing microorganism include but are not limited to using heat, antibiotics, chemicals such as iodine, bleach, ozone, and alcohols, radioactivity (i.e. irradiation), UV light, electricity, and pressure. Preferred methods of killing microorganisms are reproducible and kill at least 99% of the microorganisms. Particularly preferred is the use of heat above 50 degrees Celsius for a period of time that kills greater than 99% of the cells and preferably 100% of the cells.
Inducible Systems
In another preferred embodiment, the inventive expression of allergens by microorganisms is regulated so that synthesis occurs at a controlled time after the live microorganism is administered to an individual. Preferably the induction of protein synthesis is regulated so that activation occurs after the microorganism(s) is taken up by antigen-presenting cells (APCs) and phagocytosed into the endosome. A desirable result of this regulation is that production of the allergen of interest occurs inside the APCs and therefore reduces or eliminates the exposure of the allergen to IgE molecules bound to the surface of histamine-releasing mast cells and basophils. This reduces or eliminates the risk of anaphylaxis during administration of microorganisms that produce anaphylactic antigens.
Any method of controlling protein synthesis in the microorganism may be used in accordance with the present invention. Preferably, the method of controlling protein synthesis utilizes an inducible promoter operatively-linked to the gene of interest (e.g., a gene which encodes a signal peptide and protein antigen). Many systems for controlling transcription of a gene using an inducible promoter are known (Ausubel et al. Current Protocols in Molecular Biology. Wiley and Sons. New York. 1999). Generally, inducible systems either utilize activation of the gene or derepression of the gene. It is preferred that the present invention utilizes activation of a gene to induces transcription. However, inducible systems using derepression of a gene may also be used in the present invention. Systems using activation are preferred because these systems are able to tightly control inactivation (and hence basal level synthesis) since derepression may result in low levels of transcription if the derepression is not tight.
Methods of inducing transcription include but are not limited to induction by the presence or absence of a chemical agent, induction using a nutrient starvation inducible promoter, induction using a phosphate starvation inducible promoter and induction using a temperature sensitive inducible promoter. A particularly preferred system for regulating gene expression utilizes tetracycline controllable expression system. Systems which utilize the tetracycline controllable expression system are commercially available (see for example, Clontech, Palo Alto, Calif.).
Another particularly preferred system for regulating gene expression utilizes an ecdysone-inducible expression system which is also commercially available (Invitrogen, Carlsbad Calif.). The ecdysone-inducible expression system is based on the ability of ecdysone which is an insect hormone, to activate gene expression by binding to the ecdysone receptor. The expression system utilizes a modified heterologous protein containing the ecdysone receptor, a viral transactivation domain (from VP16) and the retinoid X receptor derived from mammalian cells to bind to a modified ecdysone response element in the presence of a ligand such as ecdysone or an analog (e.g. muristerone A, ponasterone A).
It is preferred that inducible systems for use in the present invention utilize inducing agents that are non-toxic to mammalians cells including humans. Furthermore, it is preferred that transcriptional inducing agents permeate cells membranes. More specifically for activation of protein synthesis in microorganisms after phagocytosis by APCs, transcriptional inducing agents must be able to pass through cells membranes of the APC and cell membranes of the microorganism to activate the expression of genes encoding protein allergens in accordance with the present invention. Since both tetracycline and ecdysone are able to pass through cell membranes and are non-toxic, tetracycline-inducible systems and ecdysone-inducible systems are ideally suited for use in the present invention. However, the use of inducible systems in the present invention is not limited to those systems.
It is also preferred that bacteria that have not been phagocytosed are killed before induction of genes expressing polypeptide allergens of interest. A preferred method of killing bacteria is to use antibiotics which are not permeable to mammalian cell membranes such that only bacteria that are not phagocytosed are killed. The use of antibiotics in accordance with the present embodiment reduces or eliminates the production of polypeptides by bacteria outside antigen presenting cells. It is important to reduce or eliminate exposure of allergen-producing bacteria to the immune system, especially bacteria that secrete polypeptides, which could elicit a potentially lethal anaphylactic reaction in an individual. Those having ordinary skill in the art are readily aware of antibiotics which may be used. Such antibiotics include but are not limited to penicillin, ampicillin, cephalosporin, griseofulvin, bacitracin, polymyxin b, amphotericin b, erythromycin, neomycin, streptomycin, tetracycline, vancomycin, gentamicin, and rifamycin
Secretion Signals
In another embodiment of the present invention, expressed allergens (and/or immunomodulatory molecules, such as cytokines; see below) are secreted by the microorganisms. Preferably, secretion of the allergens occurs inside a mammalian cell to reduce or eliminate exposure of allergens to a subject's allergic immune response. Secretion of polypeptides includes secretion into the extracellular medium and secretion of polypeptides into the periplasm of microorganisms such as gram-negative bacteria and yeast. Advantages of secreting allergens into the periplasm include reducing leakage of the allergens prior to phagocytosis of the microorganism. This advantage is most applicable in non-inducible systems. Advantages of secreting allergens into the extracellular medium in inducible systems include maximizing the amount of allergens available for processing by antigen-presenting cells after phagocytosis of the microorganisms of the present invention.
To express secreted polypeptides in bacteria, a variety of bacterial secretion signals known in the art may be used. For example, the Sec-dependent process in E. coli is one which is well known (for a review see Driessen et al. Curr. Opin. Microbiology 1:216-22). In addition, the OmpA signal peptide in E. coli has been described by Wong and Sutherland (U.S. Pat. No. 5,223,407). Fusion proteins containing either of these secretion signal peptides are not fully secreted by the bacteria, but rather transported across the inner membrane of the gram-negative bacteria into the periplasm. These secretion signals may be used in the present invention to transport allergenic or immunomodulatory polypeptides into the periplasm of bacteria. After administration of the genetically engineered bacteria to an individual and subsequent phagocytosis by APCs, the allergenic or immunomodulatory polypeptides in the periplasm are released after degradation of the outer membrane by enzymes in the endosome of the APCs. Preferably, the bacteria synthesize and secrete the polypeptides into the periplasm and are killed, preferably heat-killed, before administration. However, it is recognized that attenuated bacteria may be used to secrete inventive allergens into the periplasm and administered to individuals.
In another preferred embodiment of secreted proteins or polypeptides, fusion proteins containing secretion signal sequences and allergenic or immunomodulatory sequences are fully secreted into the extracellular medium by a microorganism after synthesis of the protein. Such secretion signals include those found in hemolysin and listeriolysin. In a particularly preferred embodiment, the hemolysin complex of E. coli is used to transport allergenic or immunomodulatory polypeptides across the inner and outer membrane of a microorganism (e.g. E. coli, Salmonella, Shigella, Vibrio, Yersinia, Citrobacter, Serratia, Pseudomonas) into the extracellular medium (Spreng et al. Mol. Microbiol. 31:1589-1601, 1999, and references therein all of which are incorporated herein by reference). Fusion of HlyAs to proteins and polypeptides has been shown to result in secretion of these fusion proteins utilizing the hemolysin secretion system (Blight and Holland, Trends Biotechnol. 1994 November; 12(11):450-5; Gentschev et al., Behring Inst Mitt. 1994 December; (95):57-66)
The hemolysin protein (HlyA) contains a C-terminal transport signal (HlyAs) which is approximately 50-60 amino acids in length (Hess et al., Mol Gen Genet. 1990 November; 224(2):201-8; Jarchau et al., Mol Gen Genet. 1994 Oct. 17; 245(1):53-60). The HlyA protein is secreted across the inner and outer cellular membranes by the hemolysin secretion system. This complex contains three membrane proteins. Two of these proteins, HlyB and HlyD, are located in the inner membrane, and the third TolC, is located at the outer membrane. Genes encoding these proteins are part of the hemolysin operon which consists of four genes hlyC, hlyA, hlyB, and hlyD (Wagner et al., J Bacteriol. 1983 April; 154(1):200-10; Gentschev. Gene. 1996 Nov. 7; 179(1):133-40).
In a preferred embodiment for use of the Hly secretion system, DNA plasmids (vectors) are used to express fusion proteins containing the HlyAs signal peptide and allergenic or immunomodulatory polypeptides. The genes encoding the transport complex (hlyB, and hlyD) are encoded by the same vector. It is recognized that multiple vectors can be used to encode and express these genes, or that sequences encoding these genes can be inserted into the host genome for expression. Preferably, a single vector contains the complete hemolysin operon including the hly specific promoter and an enhancer-type regulator hlyR; the HlyA gene where only the minimal polypeptide sequence necessary to transport a fusion protein is present; and the antigen of interest. TolC protein is generally produced by the host E. coli system. However, in systems where tolC DNA is not encoded by a host organism, tolC can be encoded by a vector.
In a particularly preferred embodiment, the secretion plasmid pMOhly1 described in WO 98/50067 (“Donner”) is used to express fusion proteins containing secretion signal sequences and polypeptides related to inducing anaphylaxis in individuals. The secretion vector pMOhly1 contains the complete hemolysin operon including the hly specific promoter and an enhancer-type regulator hlyR. A majority of the hlyA gene has been deleted so that HlyA encodes only the 34 amino terminal and 61 carboxyl terminal amino acids (HlyAs). A unique Nsi restriction enzyme site between the amino terminal and carboxyl terminal residues of HlyA facilitates the insertion of heterologous genes or gene fragments into the reading frame of HlyAs. The genetic information for antigens the size of 10-1000 amino acids can be inserted into this secretion vector pMOhly1, which facilitates the secretion of these antigens in attenuated Salmonella and other gram-negative attenuated inoculation strains (e.g. E. coli, Vibrio cholera, Yersina enterocolitica). In contrast to other secretion systems, the secretion of fusion proteins using a single plasmid is described by Donner. An advantage of the hemolysin secretion system in comparison to conventional transport systems is the larger size of the fusion proteins synthesized and secreted according to the methods taught in Donner. Conventional secretion systems for the presentation of antigens are only capable of secreting relatively short peptides to the outer part of the bacterial cell (Cardenas and Clements, Clin Microbiol Rev. 1992 July; 5(3):328-42).
Antigens and Allergens
In general, any allergen may be produced by microorganisms in accordance with the present invention. Preferred allergens are found in certain foods, venom, drugs or rubber and are capable of eliciting allergic responses, and in particular anaphylactic allergic responses in an individual. Particularly preferred allergens are protein or polypeptide allergens.
In a preferred embodiment, microorganisms of the present invention produce allergenic proteins that elicit allergies, possibly anaphylaxis, and are found in foods, venoms, drugs, and rubber-based products. Particularly preferred allergenic proteins that induce anaphylaxis, such as several protein allergens found in food (peanut, milk, egg, wheat), insect venom (i.e. bees, reptiles), drugs, and latex. Non-limiting examples of protein allergens found in food include proteins found in nuts (e.g., peanut walnut, almond, pecan, cashew, hazelnut, pistachio, pine nut, brazil nut), seafood (e.g. shrimp, crab, lobster, clams), fruit (e.g. plums, peaches, nectarines; Ann Allergy Asthma Immunol 7(6):504-8 (1996); cherries, Allergy 51(10):756-7 (1996)), seeds (sesame, poppy, mustard), and soy and dairy products (e.g., egg, milk).
Some protein allergens found in nuts are related to legume allergies and may be used instead of the legume proteins (e.g. peanuts, soybeans, lentils; Ann Allergy Asthma Immunol 77(6): 480-2 (1996). Also, protein antigens found in pollen-related food allergies may be used (e.g. birch pollen related to apple allergies). Other protein allergens found in foods include those found in young garlic (Allergy 54(6):626-9 (1999), and for children allergic to house dust mites, allergens found in snails (Arch Pediatr 4(8):767-9 (1997)). Protein allergens in wheat are known to cause exercise-induced allergies (J Allergy Clin Immunol 1999 May; 103(5 Pt 1):912-7).
Stings from organisms that inject venoms, such as insect stings are known to cause anaphylaxis in individuals with allergies to the venom. In general, insect venom includes venom from Hymenoptera such as bees, hornets, wasps, yellow jackets, velvet ants, and fire ants. In particular for example, venom from honey bees of the genus Apis can cause anaphylaxis in stung victims who are allergic (Weber et al. Allergy 42:464-470). The venom from honey bees contains numerous compounds which have been extensively studied and characterized (see for a reference, Banks and Shipolini. Chemistry and Pharmacology of Honey-bee Venom. Chapter 7 of Venoms of the Hymenoptera. Ed. T. Piek. Academic Press. London. 1986). The two main components of bee venom are phospholipase A2 and melittin and are preferred protein allergens for use in the present invention for treating and preventing allergies to bee venom.
In certain uses of the present invention, it will be desirable to work in systems in which a single compound (e.g., a single protein) is responsible for most observed allergy. In other cases, the invention can be applied to more complex allergens. Therefore, collections of more than one antigen can be used so that immune responses to multiple antigens may be modulated simultaneously.
Appendix A presents a representative list of certain known protein antigens. As indicated, the amino acid sequence is known for many or all of these proteins, either through knowledge of the sequence of their cognate genes or through direct knowledge of protein sequence, or both. Of particular interest are anaphylactic antigens.
In another embodiment of allergenic antigens, microorganisms are genetically engineered to synthesize and secrete modified allergenic polypeptides that elicit anaphylaxis when exposed to individuals who are susceptible to anaphylactic shock. Preferably, the allergens are modified such that the ability to elicit anaphylaxis is reduced or eliminated. As previously discussed allergens elicit allergic responses which are sometimes severe enough to induce anaphylactic shock by crosslinking IgE antibodies bound to the surface of mast cells and basophils. The IgE crosslinking releases compounds such as histamines which causes symptoms related to allergies and anaphylactic shock. In accordance with the present invention, microorganisms are used to synthesize and secrete antigens which are modified to reduce or eliminate IgE binding sites while still maintaining antigenicity or immunomodulatory activity (U.S. Ser. No. 09/141,220 incorporated herein by reference). This reduces the risk of allergic or anaphylactic responses in individuals treated with vaccines containing these engineered microorganisms.
The amount of antigen to be employed in any particular composition or application will depend on the nature of the particular antigen and of the application for which it is being used, as will readily be appreciated by those of ordinary skill in the art. The experiments described in Examples 1-4 suggest that larger amounts of polypeptides are useful for inducing Th1 responses. The amount of antigen can be controlled by a variety of factors including but not limited to expression systems, inducible expression systems, levels of secretion and excretion, methods of killing bacteria before delivery. Those of ordinary skill in the art are capable of determining the desired levels of antigens to be produced by bacteria and delivered to individuals.
It is recognized that multiple antigenic molecules may be delivered by bacteria simultaneously in accordance with the methods of the present invention. Without limitation, different antigenic determinants for one antigenic protein may be delivered. Different antigenic determinants from different antigenic proteins may also be delivered. Further, multiple antigenic polypeptides and proteins may be delivered in accordance with the present invention. It is also recognized that single or multiple antigenic polypeptides and single or multiple cytokines may be delivered to individuals by bacteria in accordance with the present invention. For example but without limitation, allergenic antigens of the present invention and immunomodulatory molecules such as interleukins may be delivered by bacteria using secreted or non-secreted methods in accordance with the present invention.
Diagnostic and Therapeutic Reagents
The first step in making the modified allergen is to identify IgE binding sites and/or immunodominant IgE binding sites. The second step is to mutate one or more of the IgE binding sites, preferably including at a minimum one of the immunodominant sites, or to react the allergen with a compound that selectively blocks binding to one or more of the IgE binding sites. The third step is to make sufficient amounts of the modified allergen for administration to persons or animals in need of tolerance to the allergen, where the modified allergen is administered in a dosage and for a time to induce tolerance, or for diagnostic purposes. The modified allergen can be administered by injection, or in some cases, by ingestion or inhalation.
A. Allergens.
Many allergens are known that elicit allergic responses, which may range in severity from mildly irritating to life-threatening. Food allergies are mediated through the interaction of IgE to specific proteins contained within the food. Examples of common food allergens include proteins from peanuts, milk, grains such as wheat and barley, soybeans, eggs, fish, crustaceans, and mollusks. These account for greater than 90% of the food allergies (Taylor, Food Techn. 39, 146-152 (1992). The IgE binding epitopes from the major allergens of cow milk (Ball, et al. (1994) Clin. Exp. Allergy, 24, 758-764), egg (Cooke, S. K. and Sampson, H. R. (1997) J. Immunol., 159, 2026-2032), codfish (Aas, K., and Elsayed, S. (1975) Dev. Biol. Stand. 29, 90-98), hazel nut (Elsayed, et al. (1989) Int. Arch. Allergy Appl. Immunol. 89, 410-415), peanut (Burks et al., (1997) Eur. J. Biochemistry, 245:334-339; Stanley et al., (1997) Archives of Biochemistry and Biophysics, 342:244-253), soybean (Herein, et al. (1990) Int. Arch. Allergy Appl. Immunol. 92, 193-198) and shrimp (Shanty, et al. (1993) J. Immunol. 151, 5354-5363) have all been elucidated, as have others Other allergens include proteins from insects such as flea, tick, mite, fire ant, cockroach, and bee as well as molds, dust, grasses, trees, weeds, and proteins from mammals including horses, dogs, cats, etc.
The majority of allergens discussed above elicit a reaction when ingested, inhaled, or injected. Allergens can also elicit a reaction based solely on contact with the skin. Latex is a well known example. Latex products are manufactured from a milky fluid derived from the rubber tree, Hevea brasiliensis and other processing chemicals. A number of the proteins in latex can cause a range of allergic reactions. Many products contain latex, such as medical supplies and personal protective equipment. Three types of reactions can occur in persons sensitive to latex: irritant contact dermatitis, and immediate systemic hypersensitivity. Additionally, the proteins responsible for the allergic reactions can fasten to the powder of latex gloves This powder can be inhaled, causing exposure through the lungs. Proteins found in latex that interact with IgE antibodies were characterized by two-dimensional electrophoresis. Protein fractions of 56, 45, 30, 20, −14, and less than 6.5 kd were detected (Posch A. et al., (1997) J. Allergy Clin. Immunol. 99(3), 385-395). Acidic proteins in the 8-14 kd and 22-24 kd range that reacted with IgE antibodies were also identified (Posch A. et al., (1997) J. Allergy Clin. Immunol. 99(3), 385-395. The proteins prohevein and hevein, from hevea brasiliensis, are known to be major latex allergens and to interact with IgE (Alenius, H., et al., Clin. Exp. Allergy 25(7), 659-665; Chen Z., et al., (1997). J. Allergy Clin. Immunol. 99(3), 402-409). Most of the IgE binding domains have been shown to be in the hevein domain rather than the domain specific for prohevein (Chen Z., et al., (1997) J. Allergy Clin. Immunol. 99(3), 402-409). The main IgE-binding epitope of prohevein is thought to be in the N-terminal, 43 amino acid fragment (Alenius H., et al., (1996) J. Immunol. 156(4), 1618-1625). The hevein lectin family of proteins has been shown to have homology with potato lectin and snake venom disintegrins (platelet aggregation inhibitors) (Kielisqewski, M. L., et al., (1994) Plant J. 5(6), 849-861).
B. Identification of IgE Binding Sites.
Allergens typically have both IgE and IgG binding sites and are recognized by T cells. The binding sites can be determined either by using phage display libraries to identify conformational epitopes (Eichler and Houghten, (1995) Molecular Medicine Today 1, 174-180; Jensen-Jarolim et al., (1997) J. Appl. Clin. Immunol. 101, 5153a) or by using defined peptides derived from the known amino acid sequence of an allergen (see examples below), or by binding of whole protein or protein fragments to antibodies, typically antibodies obtained from a pooled patient population known to be allergic to the allergen. It is desirable to modify allergens to diminish binding to IgE while retaining their ability to activate T cells and in some embodiments by not significantly altering or decreasing IgG binding capacity. This requires modification of one or more IgE binding sites in the allergen.
A preferred modified allergen is one that can be used with a majority of patients having a particular allergy. Use of pooled sera from allergic patients allows determination of one or more immunodominant epitopes in the allergen. Once some or all of the IgE binding sites are known, it is possible to modify the gene encoding the allergen, using site directed mutagenesis by any of a number of techniques, to produce a modified allergen as described below, and thereby express modified allergens. It is also possible to react the allergen with a compound that achieves the same result as the selective mutation, by making the IgE binding sites inaccessible, but not preventing the modified allergen from activating T cells, and, in some embodiments, by not significantly altering or decreasing IgG binding.
Assays to assess an immunologic change after the administration of the modified allergen are known to those skilled in the art. Conventional assays include RAST (Sampson and Albergo, 1984), ELISAs (Burks, et al. 1986) immunoblotting (Burks, et al. 1988), and in vivo skin tests (Sampson and Albergo 1984). Objective clinical symptoms can be monitored before and after the administration of the modified allergen to determine any change in the clinical symptoms.
It may be of value to identify IgEs which interact with conformational rather than linear epitopes. Due to the complexity and heterogeneity of patient serum, it may be difficult to employ a standard immobilized allergen affinity-based approach to directly isolate these IgEs in quantities sufficient to permit their characterization. These problems can be avoided by isolating some or all of the IgEs which interact with conformational epitopes from a combinatorial IgE phage display library.
Steinberger et al. (Steinberger, P., Kraft D. and Valenta R. (1996) “Construction of a combinatorial IgE library from an allergic patient: Isolation and characterization of human IgE Fabs with specificity for the major Timothy Grass pollen antigen,” Phl p. 5 J. Biol. Chem. 271, 10967-10972) prepared a combinatorial IgE phage display library from mRNA isolated from the peripheral blood mononuclear cells of a grass allergic patient. Allergen-specific IgEs were selected by panning filamentous phage expressing IgE Fabs on their surfaces against allergen immobilized on the wells of 96 well microtiter plates. The cDNAs were than isolated from allergen-binding phage and transformed into E coli for the production of large quantities of monoclonal, recombinant, allergen-specific IgE Fabs.
If native allergen or full length recombinant allergen is used in the panning step to isolate phage, then Fabs corresponding to IgEs specific for conformational epitopes should be included among the allergen-specific clones identified. By screening the individual recombinant IgE Fabs against denatured antigen or against the relevant linear epitopes identified for a given antigen, the subset of conformation-specific clones which do not bind to linear epitopes can be defined.
To determine whether the library screening has yielded a complete inventory of the allergen-specific IgEs present in patient serum, an immunocompetition assay can be performed. Pooled recombinant Fabs would be preincubated with immobilized allergen. After washing to remove unbound Fab, the immobilized allergen would then be incubated with patient serum. After washing to remove unbound serum proteins, an incubation with a reporter-coupled secondary antibody specific for IgE Fc domain would be performed. Detection of bound reporter would allow quantitation of the extent to which serum IgE was prevented from binding to allergen by recombinant Fab. Maximal, uncompeted serum IgE binding would be determined using allergen which had not been preincubated with Fab or had been incubated with nonsense Fab. If IgE binding persists in the face of competition from the complete set of allergen-specific IgE Fab clones, this experiment can be repeated using denatured antigen to determine whether the epitopes not represented among the cloned Fabs are linear or conformational.
Production of Recombinant or Modified Allergens
A modified allergen will typically be made using recombinant techniques. Expression in a procaryotic or eucaryotic host including bacteria, yeast, and baculovirus-insect cell systems are typically used to produce large (mg) quantities of the modified allergen. It is also possible to make the allergen synthetically, if the allergen is not too large, for example, less than about 25-40 amino acids in length.
Adjuvants and Immunostimulatory Agents
Compositions and methods of the present invention include the use of adjuvants and immunomodulatory polypeptides or immunostimulatory factors to modulate an individual's immune response. Immunologic adjuvants are agents that enhance specific immune responses to vaccines. Formulation of vaccines with potent adjuvants is desirable for improving the performance of vaccines composed of antigens. Adjuvants may have diverse mechanisms of action and should be selected for use based on the route of administration and the type of immune response (antibody, cell-mediated, or mucosal immunity) that is desired for a particular vaccine
In general, immunomodulatory polypeptides include cytokines which are small proteins or biological factors (in the range of 5-20 kD) that are released by cells and have specific effects on cell-cell interaction, communication and behavior of other cells. As previously described, cytokines in accordance with the present invention are proteins that are secreted to T-cells to induce a Th1 or Th2 response. Preferably, the cytokine(s) to be administered is/are selected to reduce production of a Th2 response to antigens associated with anaphylaxis. One preferred method of reducing a Th2 response is through induction of the alternative response. Cytokines that, when expressed during antigen delivery into cells, induce a Th1 response in T cells (i.e., “Th1 stimulating cytokines”) include IL-12, IL-2, I-18, IL-1 or fragments thereof, IFN, and/or IFNγ.
Other compounds that are immunomodulatory include immunological inducing agents. These inducing agents prompt the expression of Th1 stimulating cytokines by T-cells and include factors such as, CD40, CD40 ligand, oligonucleotides containing CpG motifs, TNF, and microbial extracts such as preparations of Staphylococcus aureus, heat killed Listeria, and modified cholera toxin, etc.
Those of ordinary skill in the art readily appreciate the preferred types of adjuvants for use with particular antigen compositions. In general, immunologic adjuvant include gel-type adjuvants (e.g. aluminum hydroxide/aluminum phosphate, calcium phosphate), microbial adjuvants (e.g. DNA such as CpG motifs; endotoxin such as monophosphoryl lipid A; exotoxins such as cholera toxin, E. coli heat labile toxin, and pertussis toxin; and muramyl dipeptide), oil-emulsion and emulsifier-based adjuvants (e.g. Freund's Incomplete Adjuvant, MF59, and SAF), particulate adjuvants (e.g. liposomes, biodegradable microspheres, and saponins), and synthetic adjuvants (e.g. nonionic block copolymers, muramyl peptide analogues, polyphosphazene, and synthetic polynucleotides).
Adjuvants that are known to stimulate Th2 responses are preferably avoided. Particularly preferred adjuvants include, for example, preparations (including heat-killed samples, extracts, partially purified isolates, or any other preparation of a microorganism or macroorganism component sufficient to display adjuvant activity) of microorganisms such as Listeria monocytogenes or others (e.g., Bacille Calmette-Guerin [BCG], Corynebacterium species, Mycobacterium species, Rhodococcus species, Eubacteria species, Bortadella species, and Nocardia species), and preparations of nucleic acids that include unmethylated CpG motifs (see, for example, U.S. Pat. No. 5,830,877; and published PCT applications WO 96/02555, WO 98/18810, WO 98/16247, and WO 98/40100, each of which is incorporated herein by reference). Other preferred adjuvants reported to induce Th1-type responses and not Th2-type responses include, for example, Aviridine (N,N-dioctadecyl-N′N′-bis(2-hydroxyethyl) propanediamine) and CRL 1005. Particularly preferred are ones that induce IL-12 production, including microbial extracts such as fixed Staphylococcus aureus, Streptococcal preparations, Mycobacterium tuberculosis, lipopolysaccharide (LPS), monophosphoryl lipid A (MPLA) from gram negative bacterial lipopolysaccharides (Richards et al. Infect Immun 1998 June; 66(6):2859-65), listeria monocytogenes, toxoplasma gondii, leishmania major. Some polymers are also adjuvants. For example, polyphosphazenes are described in U.S. Pat. No. 5,500,161 to Andriavnov, et al. These can be used not only to encapsulate the microorganisms but also to enhance the immune response to the antigen.
If adjuvants are not synthesized by microorganisms in accordance with the present invention, adjuvants which are cytokines may be provided as impure preparations (e.g., isolates of cells expressing a cytokine gene, either endogenous or exogenous to the cell), but are preferably provided in purified form. Purified preparations are preferably at least about 90% pure, more preferably at least about 95% pure, and most preferably at least about 99% pure. Alternatively, genes encoding the cytokines or immunological inducing agents may be provided, so that gene expression results in cytokine or immunological inducing agent production either in the individual being treated or in another expression system (e.g., an in vitro transcription/translation system or a host cell) from which expressed cytokine or immunological inducing agent can be obtained for administration to the individual. It is recognized that microorganisms utilized to synthesize and deliver allergenic and/or immunomodulatory proteins according to the present invention can act as an adjuvant, and that preferred microorganisms are immunostimulatory adjuvants.
It will be appreciated by those of ordinary skill in the art that the inventive administration of microorganisms expressing cytokines and/or allergens may optionally be combined with the administration of any other desired immune system modulatory factor such as, for example, an adjuvant or other immunomodulatory compound.
Methods of Administration
Formulations can be delivered to a patient by any available route including for example enteral, parenteral, topical (including nasal, pulmonary or other mucosal route), oral or local administration. The compositions are preferably administered in an amount effective to elicit cellular immunity and production of Th1-related IgG while minimizing IgE mediated responses. Also preferred are compositions administered in an effective amount to active T-cell response, preferably Th1-type responses. For compositions of the present invention containing bacteria, administration is preferably delivered parenterally.
It is important to administer the modified allergen to an individual (human or animal) to decrease the clinical symptoms of allergic disease by using a method, dosage, and carrier which are effective. Allergen will typically be administered in an appropriate carrier, such as saline or a phosphate saline buffer. Allergen can be administered by injection subcutaneously, intramuscularly, or intraperitoneally (most humans would be treated by subcutaneous injection), by aerosol, inhaled powder, or by ingestion.
Pharmaceutical Compositions
Pharmaceutical compositions for use in accordance with the present invention may include a pharmaceutically acceptable excipient or carrier. As used herein, the term “pharmaceutically acceptable carrier” means a non-toxic, inert solid, semi-solid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type. Some examples of materials which can serve as pharmaceutically acceptable carriers are sugars such as lactose, glucose, and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil; safflower oil; sesame oil; olive oil; corn oil and soybean oil; glycols; such as propylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol, and phosphate buffer solutions, as well as other non-toxic compatible lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the composition, according to the judgment of the formulator. The pharmaceutical compositions of this invention can be administered to humans and/or to other animals, orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, or as an oral or nasal spray.
Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include agents such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.
In order to prolong the effect of an agent, it is often desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the agent then depends upon its rate of dissolution which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the drug in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of agent to polymer and the nature of the particular polymer employed, the rate of release of the agent can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides) Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions which are compatible with body tissues.
Compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents.
Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes.
Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
The compounds can also be in micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes.
Dosage forms for topical or transdermal administration of an inventive pharmaceutical composition include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, ear drops, eye drops are also contemplated as being within the scope of this invention.
The ointments, pastes, creams and gels may contain, in addition to an active compound of this invention, excipients such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.
Powders and sprays can contain, in addition to the compounds of this invention, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances. Sprays can additionally contain customary propellants such as chlorofluorohydrocarbons.
Transdermal patches have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms can be made by dissolving or dispensing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.
Encapsulation
In a preferred embodiment, inventive compositions comprising live microorganisms are provided in association with an encapsulation device (see, for example, U.S. Ser. No. 60/169,330 entitled “Controlled Delivery of Antigens” filed Dec. 6, 1999, incorporated by reference herewith). Preferred encapsulation devices are biocompatible, are stable inside the body so that microorganisms are not released until after the encapsulation device reaches its intended destination (e.g. mucosal lining of the gut, endocytosis by antigen-presenting cells (APC)). For example, preferred systems of encapsulation are stable at physiological pH and degrade at acidic pH levels comparable to those found in the digestive tract or endosomes of APCs. Particularly preferred encapsulation compositions include but are not limited to ones containing liposomes, polylactide-co-glycolide (PLGA), chitosan, synthetic biodegradable polymers, environmentally responsive hydrogels, and gelatin PLGA nanoparticles. Inventive compositions may be encapsulated in combination with one or more adjuvants, targeting entities, or other agents including, for example, pharmaceutical carriers, diluents, excipients, oils, etc. Alternatively or additionally the encapsulation device itself may be associated with a targeting entity and/or an adjuvant.
Methods of encapsulating live cells are known and may also be used in accordance with the present invention for delivering antigen-secreting microorganisms to individuals. The following references are provided as examples of encapsulation of live cells. However, any method of encapsulating live cells may be used in the present invention. U.S. Pat. No. 5,084,350; U.S. Pat. No. 4,680,174; and U.S. Pat. No. 4,352,883 (all of which are incorporated herein by reference) describe the encapsulation of a prokaryotic or eukaryotic cell or cell culture in microcapsules. Briefly, U.S. Pat. Nos. 5,084,350; 4,680,174; and 4,352,883 disclose that a tissue sample, cell, or cell culture to be encapsulated is first prepared in finely divided form in accordance with well-known techniques and suspended in an aqueous medium suitable for maintenance and for supporting the ongoing metabolic processes of the particular cells involved. Media suitable for this purpose generally are available commercially. Thereafter, a water-soluble substance which is physiologically compatible with the cells and which can be rendered water-insoluble to form a shape-retaining coherent spheroidal mass or other shape is added to the medium. The solution is then formed into droplets containing cells together with their maintenance or growth medium and is immediately rendered water-insoluble and gelled to form shape-retaining, typically spheroidal coherent masses.
The material used to induce gelation of the culture medium may be any non-toxic water-soluble material which, by a change in the surrounding temperature, pH, ionic environment, or concentration, can be converted to shape-retaining masses. Preferably, the material also is one which comprises plural, easily ionized groups, e.g., carboxyl or amino groups, which can react by salt formation with polymers containing plural groups which ionize to form species of the opposite charge. Use of this type of material enables the deposition of a membrane of a selected porosity range without damage to the labile cells. The presently preferred materials for forming the gelled masses are water-soluble natural or synthetic polysaccharides. Many such commercially available materials are typically extracted from vegetable matter and are often used as additives in various foods. Sodium alginate is the presently preferred water-soluble polysaccharide. Other usable materials include acidic fractions of guar gum, gum arabic, carrageenan, pectin, tragacanth gum or xanthan gums. These materials may be gelled when multivalent ions are exchanged for the acidic hydrogen or alkali metal ion normally associated with the carboxyl groups.
Uses
The compositions of the present invention may be employed to treat or prevent allergic reactions in a subject. Subjects are animal and human patients in need of treatment for allergies. Preferably, the animal is a domesticated mammal (e.g., a dog, a cat, a horse, a sheep, a pig, a goat, a cow, etc). Animals also include laboratory animals such as mice, rats, hamsters, monkeys, and rabbits. Any individual who suffers from allergy, or who is susceptible to allergy, may be treated. It will be appreciated that an individual can be considered susceptible to allergy without having suffered an allergic reaction to the particular antigen in question. For example, if the individual has suffered an allergic reaction to a related antigen (e.g., one from the same source or one for which shared allergies are common), that individual will be considered susceptible to allergy to the relevant antigen. Similarly, if members of an individual's family are allergic to a particular antigen, the individual may be considered to be susceptible to allergy to that antigen. More preferably, any individual who is susceptible to anaphylactic shock upon exposure to food allergens, venom allergens or rubber allergens may be treated according to the present invention.
The compositions of the present invention may be formulated for delivery by any route. Preferably, the compositions are formulated for injection, ingestion, or inhalation.
Therapy or desensitization with the modified allergens can be used in combination with other therapies, such as allergen-non-specific anti-IgE antibodies to deplete the patient of allergen-specific IgE antibodies (Boulet, et al. (1997) 155:1835-1840; Fahy, et al. (1997) American J Respir. Crit. Care Med. 155:1828-1834; Demoly, P. and Bousquet (1997) J Am J. Resp. Crit. Care Med. 155:1825-1827), or by the pan specific anti-allergy therapy described in U.S. Ser. No. 08/090,375 filed Jun. 4, 1998, by M. Caplan and H. Sosin. Therapy with the modified allergen can also be administered in combination with an adjuvant such as IL-12, IL-16, IL-18, IFNγ.
The nucleotide molecule encoding the modified allergen can also be administered directly to the patient, for example, in a suitable expression vector such as a plasmid, which is injected directly into the muscle or dermis, or through administration of genetically engineered cells.
In general, effective dosages will be in the picogram to milligram range, more typically microgram to milligram. Treatment will typically be between twice/weekly and once a month, continuing for up to three to five years, although this is highly dependent on the individual patient response.
The modified allergen can also be used as a diagnostic to characterize the patient's allergies, using techniques such as those described in the examples.
Modifications and variations of the methods and compositions described herein are intended to be within the scope of the following claims.
Other Embodiments
Those of ordinary skill in the art will readily appreciate that the foregoing represents merely certain preferred embodiments of the invention. Various changes and modifications to the procedures and compositions described above can be made without departing from the spirit or scope of the present invention, as set forth in the following claims.
EXAMPLES
Material and Methods
For general methods used to express proteins in microorganisms see Ausubel et al. (supra) and Sambrook et al. (supra) both of which are incorporated herein by reference. In addition, expression vectors for use in the present invention are widely available from commercial sources (see for example, Clontech, Palo Alto, Calif.; Invitrogen, Carlsbad, Calif.; Promega Corporation, Madison, Wis.; New England Biolabs, Beverly, Mass.).
The following experiments describe the encapsulation of allergens in bacteria for use as a delivery vehicle and/or adjuvant in immunotherapy in accordance with the teachings of the present invention. Recombinant peanut allergen proteins (Ara h 1, Ara h 2, and Ara h 3; Burks et al. J Allergy Clin Immunol. 88(2):172-9, 1991; Burks et al. J Allergy Clin Immunol. 90(6 Pt 1):962-9, 1992; Rabjohn et al. J Clin Invest. 103(4):535-42, 1999; incorporated herein by reference) were produced in E. coli BL21 cells by transforming the bacterial cells with cDNA clones encoding the proteins (see Appendix B; sequences cloned into pET24, Novagen, Madison, Wis.). The transformed cells were then injected into C3H/HEJ mice to determine if the allergen-expressing E. coli elicited an immune response.
Example 1
Methods of Killing Allergen-Producing E. coli 
Several methods of killing allergen-producing E. coli were tested. Preferably, the method of killing bacteria does not denature or proteolyze the recombinant allergen(s) produced by the bacteria. As non-limiting examples, E. coli were killed by heat (at temperatures ranging from 37° C. to 95° C.), by using ethanol (0.1% to 10%), and by using solutions containing iodine (0.1% to 10%). Survival was determined by plating 100 μl of cells onto the appropriate agar plates, and subsequently counting the resulting colonies. The most reproducible method was heat killing. Therefore, the preferred method of killing allergen-producing E. coli is to incubate the cells at 60° C. for 20 minutes which results in 100% death (i.e. no colonies formed; see FIG. 1).
Example 2
Growth of Bacteria
The following protocol was developed for the preparation of allergen-producing E. coli cells for inoculation of mice.
Day 1
Five milliliters (ml) of liquid cultures of LB (Luria-Bertani broth) containing kanamycin (30 micrograms/ml per each cell line used) were prepared in 50 ml sterile tubes or flasks. Cultures were inoculated with approximately 10 microliters from a frozen stock of the desired bacterial cell line containing the desired expression vectors. The inoculated cultures were incubated with shaking overnight at 37° C.
Day 2
The following morning, 100 ml of liquid LB (500 ml Erlenmeyer flask) containing kanamycin (30 micrograms/ml) were inoculated using a 1 ml aliquot from the 5 ml culture grown from the previous day. (The remaining 4 mls of culture were frozen. Optionally, the remaining 4 milliliters of culture can be stored at 4° C. for several weeks for inoculating subsequent cultures) The inoculated cultures were incubate with shaking at 37° C. until the optical density of the solution measured at 600 nM (OD600) reached approximately 0.6 to 0.9.
Day 3
To induce production of recombinant proteins, the cultures from the previous day were induced by adding isopropyl-beta-D-thiogalactopyranoside (IPTG; Sigma-Aldrich, St. Louis, Mo.) from a 1 M stock to a final concentration of 1 mM (100 microliters of 1 M IPTG per 100 mls of culture) when the OD600 of the culture reached approximately 0.6-0.9. The induced cultures were incubated overnight.
Day 4
1.4 ml of culture from the previous day were aliquoted into each of five 1.5 ml microfuge tubes for each culture and heat killed at 60° C. in a water bath for 20 minutes. The tubes were centrifuged at 16,000×g for 5 minutes at room temperature and the supernatant discarded. The pellets were washed with 1× phosphate buffer saline (PBS) and centrifuged at 16,000×g for 5 minutes at room temperature. Again, the supernatant was discarded and the pellets were resuspended in 250 microliters of 1×PBS. The resuspended pellets from the same original samples were combined. The OD600 were determined for each sample and diluted to the desired OD600 using 1×PBS.
Example 3
Production and Release of Allergen
Release of Allergen by Heat-Killed Bacteria
In order to determine if the cells remained intact after heat-killing we measured the amount of allergen released into the media. A dot-blot assay was developed that utilized as controls, purified recombinant allergens applied to a filter at known concentrations and serum IgE from peanut sensitive patients. The assay detected and quantified the amount of allergen present in 100 microliters of supernatant after pelleting heat-killed bacteria. The level of allergen released varied and was dependent on the expression vector and protein tested. In general, more Ara h 2 was released than Ara h 1 and Ara h 3 (Ara h 2>>Ara h 1>Ara h 3).
Production of Allergen
In order to measure amounts of allergen in E. coli, we developed an immunoblot assay that utilizes a six histidine tag (HIS tag) that is present on all of our purified recombinant allergens and a HIS tag antibody to build a standard curve that could then be used to estimate amounts of allergen produced. The amount of allergen produced on a per cell basis varied depending on which clone was tested. In general, more Ara h 3 was produced than Ara h 2 and Ara h 1 (Ara h 3>Ara h 2>>Ara h1).
Our best estimates for amounts of allergen delivered in 100 μl of a 2.0 O.D. inoculum of E. coli varies from about 1 μg of Ara h 1 to about 20 μg of Ara h 3.
FIG. 2 is an example of a standard curve generated for Ara h 2. The optical density (O.D.) of the HIS-tagged Ara h 2 allergen is then determined from an immunoblot where different concentrations of E. coli extract has been electrophoresed on SDS-PAGE gels. The allergen O.D. is then used to estimate the amount of protein produced by that extract.
Example 4
Immune Response of Mice
The following protocol was utilized to determine the immune response of mice injected with allergen-producing bacteria. Blood was collected from the tail vein of each mouse used before the first injection. Enough blood was collected for antibody ELISA for each allergen and E. coli proteins. On Day Zero each mouse was injected with 100 microliters of the killed E. coli samples subcutaneously in the left hind flank. The mice were injected for the second time on Day 14 using the same procedure as Day Zero. On Day 21, a second blood sample was collected from each mouse. Blood samples at Day 0 and Day 21 were assayed for IgG1 and IgG2a antibodies to either Ara h 1, Ara h 2, or Ara h 3 by an ELISA assay.
Mice injected with E. coli producing Ara h 1 did not give detectable levels of any immunoglobulin to the Ara h 1 allergen and therefore, that data are not shown. Without limitation to theory, we speculate that this may be due to the relatively small amounts of Ara h 1 produced by these cells (see previous discussion). Mice injected with E. coli producing Ara h 2 contained relatively high levels of IgG1 and IgG2a. Again, without limitation to the cause, we speculated that this may be due to the amount of Ara h 2 released from these cells (see discussion above). Mice injected with E. coli producing Ara h 3 contained relatively high levels of IgG2a (indicative of a Th1-type response) and elicited relatively low levels of IgG1 (indicative of a Th2-type response.
Interpretation of Results
The present data should be cautiously interpreted. The data in the Figures only represent O.D. levels and do not represent absolute amounts of immunoglobulin. Therefore comparisons between groups should take into consideration the data presented as O.D. However, the general trend suggests that for example, more mice exhibited an IgG2a response to Ara h 3 than mice that exhibit an IgG1 response to Ara h 3.
Examples 5-9
Peanut allergy is one of the most common and serious of the immediate hypersensitivity reactions to foods in terms of persistence and severity of reaction. Unlike the clinical symptoms of many other food allergies, the reactions to peanuts are rarely outgrown, therefore, most diagnosed children will have the disease for a lifetime (Sampson, H. A., and Burks, A. W. (1996) Annu. Rev. Nutr. 16, 161-77; Bock, S. A. (1985). J. Pediatr. 107, 676-680). The majority of cases of fatal food-induced anaphylaxis involve ingestion of peanuts (Sampson et al., (1992) NEJM 327, 380-384; Kaminogawa, S. (1996) Biosci. Biotech. Biochem. 60, 1749-1756). The only effective therapeutic option currently available for the prevention of a peanut hypersensitivity reaction is food avoidance. Unfortunately, for a ubiquitous food such as a peanut, the possibility of an inadvertent ingestion is great.
The examples described below demonstrate identification, modification, and assessment of allergenicity of the major peanut allergens, Ara h 1, Ara h 2, and Ara h 3. Detailed experimental procedures are included for Example 1. These same procedures were used for Examples 2-5. The nucleotide sequences of Ara h 1, Ara h 2, and Ara h 3, are shown in SEQ ID NOs. 1, 3, and 5, respectively. The amino acid sequences of Ara h 1, Ara h 2, and Ara h 3 are shown in SEQ ID NOs. 2, 4, and 6 respectively.
Example 5
Identification of Linear IgE Binding Epitopes
Due to the significance of the allergic reaction and the widening use of peanuts as protein extenders in processed foods, there is increasing interest in defining the allergenic proteins and exploring ways to decrease the risk to the peanut-sensitive individual. Various studies over the last several years have identified the major allergens in peanuts as belonging to different families of seed storage proteins (Burks, et al. (1997) Eur. J. Biochem. 245, 334-339; Stanley, et al. (1997) Arch. Biochem. Biophys. 342, 244-253). The major peanut allergens Ara h 1, Ara h 2, and Ara h 3 belong to the vicilin, conglutin and glycinin families of seed storage proteins, respectively. These allergens are abundant proteins found in peanuts and are recognized by serum IgE from greater than 95% of peanut sensitive individuals, indicating that they are the major allergens involved in the clinical etiology of this disease (Burks, et al. (1995). J. Clinical Invest., 96, 1715-1721). The genes encoding Ara h 1 (SEQ ID NO. 1), Ara h 2 (SEQ ID NO. 3), and Ara h 3 (SEQ ID NO. 5) and the proteins encoded by these genes (SEQ ID NOs. 2, 4, 6) have been isolated and characterized. The following studies were conducted to identify the IgE epitopes of these allergens recognized by a population of peanut hypersensitive patients and a means for modifying their affinity for IgE.
Experimental Procedures
Serum IgE. Serum from 15 patients with documented peanut hypersensitivity reactions (mean age, 25 yrs) was used to determine relative binding affinities between wild-type and mutant Ara h 1 synthesized epitopes. The patients had either a positive double-blind, placebo-controlled, food challenge or a convincing history of peanut anaphylaxis (laryngeal edema, severe wheezing, and/or hypotension; Burks, et al. (1988) J. Pediatr. 113, 447-451). At least 5 ml of venous blood was drawn from each patient, allowed to clot, and serum was collected A serum pool from 12 to 15 patients was made by mixing equal aliquots of serum IgE from each patient. The pools were then used in immunoblot analysis.
Peptide synthesis. Individual peptides were synthesized on a derivatized cellulose membrane using 9-fluorenyllmethoxycarbonyl (Fmoc) amino acid active esters according to the manufacturer's instructions (Geriosys Biotechnologies, Woodlands, Tex.; Fields, G. B and Noble, R. L. (1990) Int. J. Peptide Protein Res. 35, 161-214). Fmoc-amino acids (N-terminal blocked) with protected side chains were coupled in the presence of 1-methyl-2-pyrrolidone to a derivatized cellulose membrane. Following washing with dimethylformamide (DMF), unreacted terminal amino groups were blocked from further reactions by acetylation with acetic anhydride. The N-terminal Fmoc blocking group was then removed by reaction with 20% piperidine and 80% DMF, v/v. The membrane was washed in DMF followed by methanol, the next reactive Fmoc-amino acid was then coupled as before, and the sequence of reactions was repeated with the next amino acid. When peptide synthesis was complete, the side chains were deprotected with a mixture of dichloromethane (DCM), trifluoroacetic acid, and triisobutylsilane (1.0:1.0:0.5), followed by successive washes in DCM, DMF, and methanol. Peptides synthesis reactions were monitored by bromophenol blue color reactions during certain steps of synthesis. Cellulose derivitised membranes and Fmoc-amino acids were supplied by Genosys Biotechnologies. All other chemical were purchased from Aldrich Chemical Company, Inc. (Milwaukee, Wis.) or Fluka (Bucks, Switzerland). Membranes were either probed immediately or stored at −20° C. until needed.
IgE binding assays. Cellulose membranes containing synthesized peptides were washed 3 times in Tris-buffered saline (TBS; 136 mM NaCl, 2.7 mM KCl, and 50 mM trizma base pH 8.0) for 10 min at room temperature (RT) and then incubated overnight in blocking buffer: [TBS, 0.05% TweenTM 20; concentrated membrane blocking buffer supplied by Genosys; and sucrose (0.0:1.0:0.5)]. The membrane was then incubated in pooled sera diluted in 1:5 in 20 mM Tris-Cl pH7.5, 150 mM NaCl, and 1% bovine serum albumin overnight at 4° C. Primary antibody was detected with 125I-labeled equine anti-human IgE (Kallestad, Chaska, Minn.).
Quantitation of IgE binding. Relative amounts of IgE binding to individual peptides were determined by a Bio-Rad (Hercules, Calif.) model GS-700 imaging laser densitometer and quantitated with Bio-Rad molecular analyst software. A background area was scanned and subtracted from the obtained values. Following quantitation, wild-type intensities were normalized to a value of one and the mutants were calculated as percentages relative to the wild-type.
Synthesis and purification of recombinant Ara h 2 protein. cDNA encoding Ara h 2 was placed in the pET-24b expression vector. The pET-24 expression vector places a 6× histidine tag at the carboxyl end of the inserted protein. The histidine tag allows the recombinant protein to be purified by affinity purification on a nickel column (HisBind resin). Recombinant Ara h 2 was expressed and purified according to the instructions of the pET system manual. Briefly, expression of the recombinant Ara h 2 was induced in 200 ml cultures of strain BL21(DE3) E. coli with 1 mM IPTG at mid log phase. Cultures were allowed to continue for an additional 3 hours at 36° C. Cells were harvested by centrifugation at 2000×g for 15 minutes and then lysed in denaturing binding buffer (6 M urea, 5 mM imidazole, 0.5 M NaCl, 20 mM Tris-HCl, pH 7.9). Lysates were cleared by centrifugation at 39,000×g for 20 minutes followed by filtration though 0.45 micron filters. The cleared lysate was applied to a 10 ml column of HisBind resin, washed with imidazole wash buffer (20 mM imidazole, 6 M urea, 0.5 M NaCl, 20 mM Tris-HCl, pH 7.9). The recombinant Ara h 2 was then released from the column using elution buffer (1 M imidazole, 0.5 M NaCl, 20 mM Tris-HCl, pH 7.9). The elution buffer was replaced with phosphate buffered saline by dialysis. The purification of recombinant Ara h 2 was followed by SIDS PAGE and immunoblots. Peanut specific serum IgE was used as a primary antibody.
Skin prick tests. The ability of purified native and recombinant Ara h 2 to elicit the IgE mediated degranulation of mast cells was evaluated using prick skin tests in a peanut allergic individual. An individual meeting the criteria for peanut allergy (convincing history or positive double blind placebo controlled food challenge) and a non-allergic control were selected for the testing. Purified native and recombinant Ara h 2 and whole peanut extract (Greer Laboratories, Lenoir, N.C.) were tested. Twenty microliters of the test solution were applied to the forearm of the volunteer and the skin beneath pricked with a sterile needle. Testing was started at the lowest concentration (less than or equal to 1 mg/ml) and increased ten fold each round to the highest concentration or until a positive reaction was observed. Mean diameters of the wheal and erythema were measured and compared to the negative saline control. A positive reaction was defined as a wheal 3 mm larger then the negative control. Histamine was used as the positive control.
Results
Identification of the linear IgE-binding epitopes of Ara h 1, Ara h 2 and Ara h 3 allergens. Epitope mapping was performed on the Ara h 1, Ara h 2 and Ara h 3 allergens by synthesizing each of these proteins in 15 amino acid long overlapping peptides that were offset from each other by 8 amino acids. The peptides were then probed with a pool of serum IgE from 15 patients with documented peanut hypersensitivity. This analysis resulted in multiple IgE binding regions being identified for each allergen. The exact position of each IgE binding epitope was then determined by re-synthesizing these IgE reactive regions as 10 or 15 amino acid long peptides that were offset from each other by two amino acids. These peptides were probed with the same pool of serum IgE from peanut sensitive patients as used before. An example of this procedure for each of the peanut allergens is shown in FIGS. 5-7. FIG. 5 depicts twenty-two 10-mer peptides (SEQ ID NOs. 45-66) that span amino acid residues 82-133 (SEQ ID NO. 44) of the Ara h 1 allergen (SEQ ID NO. 2). This region of the Ara h 1 allergen includes epitopes 4, 5, 6, and 7, as identified in Table 1. FIG. 6 depicts seven 10-mer peptides (SEQ ID NOs. 68-74) that span amino acid residues 55-76 (SEQ ID NO. 67) of the Ara h 2 allergen (SEQ ID NO. 4). This region of the Ara h 2 allergen includes epitopes 6 and 7 as identified in Table 2. FIG. 7 depicts six 15-mer peptides (SEQ ID NOs. 76-81) that span amino acid residues 299-321 (SEQ ID NO. 75) of the Ara h 3 allergen (SEQ ID NO. 6). This region of the Ara h 3 allergen includes epitope 4 as identified in Table 3. This analysis revealed that there were 23 linear IgE binding epitopes on Ara h 1, 10 epitopes on Ara h 2, and 4 epitopes on Ara h 3.
In an effort to determine which, if any, of the epitopes were recognized by the majority of patients with peanut hypersensitivity, each set of epitopes identified for the peanut allergens were synthesized and then probed individually with serum IgE from 10 different patients. All of the patient sera tested recognized multiple epitopes.
Table 1 shows the amino acid sequence and position of each epitope within the Ara h 1 protein (SEQ ID NO. 2) of all 23 IgE binding epitopes mapped to this molecule. Table 2 shows the amino acid sequence and position of each epitope within the Ara h 2 protein (SEQ ID NO. 4) of all 10 IgE binding epitopes mapped to this molecule. Table 3 shows the amino acid sequence and position of each epitope within the Ara h 3 protein (SEQ ID NO. 6) of all 4 IgE binding epitopes mapped to this molecule.
Four epitopes of the Ara h 1 allergen (peptides 1, 3, 4, 17 of Table 1), three epitopes of the Ara h 2 allergen (peptides 3, 6, 7 of Table 2), and one epitope of the Ara h 3 allergen (peptide 2 of Table 3) were immunodominant.







TABLE 1







Ara h 1 IgE binding epitopes










EPITOPE
AA SEQUENCE
POSITION
SEQ ID NO.













1
AKSSPYQKKT
25-34
7


2
QEPDDLKQKA
48-57
8


3
LEYDPRLVYD
65-74
9


4
GERTRGRQPG
89-98
10


5
PGDYDDDRRQ
 97-106
11


6
PRREEGGRWG
107-116
12


7
REREEDWRQP
123-132
13


8
EDWRRPSHQQ
134-143
14


9
QPRKIRPEGR
143-152
15


10
TPGQFEDFFP
294-303
16


11
SYLQEFSRNT
311-320
17


12
FNAEFNEIRR
325-334
18


13
EQEERGQRRW
344-353
19


14
DITNPINLRE
393-402
20


15
NNFGKLFEVK
409-418
21


16
GTGNLELVAV
461-470
22


17
RRYTARLKEG
498-507
23


18
ELHLLGFGIN
525-534
24


19
HRIFLAGDKD
539-548
25


20
IDQIEKQAKD
551-560
26


21
KDLAFPGSGE
559-568
27


22
KESHFVSARP
578-587
28


23
PEKESPEKED
597-606
29









The underlined portions of each peptide are the smallest IgE binding sequences as determined by this analysis. All of these sequences can be found in SEQ ID NO. 2.







TABLE 2







Ara h 2 IgE binding epitopes










EPITOPE
AA SEQUENCE
POSITION
SEQ ID NO.













1
HASARQQWEL
15-24
30


2
QWELQGDRRC
21-30
31


3
DRRCQSQLER
27-36
32


4
LRPCEQHLMQ
39-48
33


5
KIQRDEDSYE
49-58
34


6
YERDPYSPSQ
57-66
35


7
SQDPYSPSPY
65-74
36


8
DRLQGRQQEQ
115-124
37


9
KRELRNLPQQ
127-136
38


10
QRCDLDVESG
143-152
39









The underlined portions of each peptide are the smallest IgE binding sequences as determined by this analysis. All of these sequences can be found in SEQ ID NO. 4.







TABLE 3







Ara h 3 IgE binding epitopes










EPITOPE
AA SEQUENCE
POSITION
SEQ ID NO.













1
IETWNPNNQEFECAG
33-47
40


2
GNIFSGFTPEFLEQA
240-254
41


3
VTVRGGLRILSPDRK
279-293
42


4
DEDEYEYDEEDRRRG
303-317
43









The underlined portions of each peptide are the smallest IgE binding sequences as determined by this analysis. All of these sequences can be found in SEQ ID NO. 6.
Example 6
Modification of Peanut Allergens to Decrease Allergenicity
The major linear IgE binding epitopes of the peanut allergens were mapped using overlapping peptides synthesized on an activated cellulose membrane and pooled serum IgE from 15 peanut sensitive patients, as described in Example 1. The size of the epitopes ranged from six to fifteen amino acids in length. The amino acids essential to IgE binding in each of the epitopes were determined by synthesizing duplicate peptides with single amino acid changes at each position. These peptides were then probed with pooled serum IgE from 15 patients with peanut hypersensitivity to determine if the changes affected peanut-specific IgE binding. For example, epitope 9 in Table 1 was synthesized with an alanine or methionine residue substituted for one of the amino acids and probed. The following amino acids were substituted (first letter is the one-letter amino acid code for the residue normally at the position, the residue number, followed by the amino acid that was substituted for this residue; the numbers indicate the position of each residue in the Ara h 1 protein, SEQ ID NO. 2): □143A, P144A; R145A; K146A; 1147A; R148A; P149A; E150A; G151A; R152A; Q143M; P144M; R145M; K146M; 1147M; R148M; P149M; E150M; G151M; R152M. The immunoblot strip containing the wild-type and mutated peptides of epitope 9 showed that binding of pooled serum IgE to individual peptides was dramatically reduced when either alanine or methionine was substituted for each of the amino acids at positions 144, 145, and 147-150 of Ara h 1 shown in SEQ ID NO. 2. Changes at positions 144, 145, 147, and 148 of Ara h 1 shown in SEQ ID NO. 2 had the most dramatic effect when methionine was substituted for the wild-type amino acid, resulting in less than 1% of peanut specific IgE binding to these peptides. In contrast, the substitution of an alanine for arginine at position 152 of Ara h 1 shown in SEQ ID NO. 2 resulted in increased IgE binding. The remaining Ara h 1 epitopes, and the Ara h 2 and Ara h 3 epitopes, were tested in the same manner and the intensity of IgE binding to each spot was determined as a percentage of IgE binding to the wild-type peptide. Any amino acid substitution that resulted in less than 1% of IgE binding when compared to the wild-type peptide was noted and is indicated in Tables 4-6. Table 4 shows the amino acids that were determined to be critical to IgE binding in each of the Ara h 1 epitopes. Table 5 shows the amino acids that were determined to be critical to IgE binding in each of the Ara h 2 epitopes. Table 6 shows the amino acids that were determined to be critical to IgE binding in each of the Ara h 3 epitopes.
This analysis indicated that each epitope could be mutated to a non-IgE binding-peptide by the substitution of a single amino acid residue.
The results discussed above for Ara h 1, Ara h 2, and Ara h 3 demonstrate that once an IgE binding site has been identified, it is possible to reduce IgE binding to this site by altering a single amino acid of the epitope. The observation that alteration of a single amino acid leads to the loss of IgE binding in a population of peanut-sensitive individuals is significant because it suggests that while each patient may display a polyclonal IgE reaction to a particular allergen, IgE from different patients that recognize the same epitope must interact with that epitope in a similar fashion. Besides finding that many epitopes contained more than one residue critical for IgE binding, it was also determined that more than one residue type (ala or met) could be substituted at certain positions in an epitope with similar results. This allows for the design of a hypoallergenic protein that would be effective at blunting allergic reactions for a population of peanut sensitive individuals. Furthermore, the creation of a plant producing a peanut where the IgE binding epitopes of the major allergens have been removed should prevent the development of peanut hypersensitivity in individuals genetically predisposed to this food allergy.







TABLE 4







Amino acids critical to IgE binding of Ara h 1










EPITOPE
AA SEQUENCE
POSITION
SEQ ID NO.













1
AKSSPYQKKT
23-34
7


2
QEPDDLKQKA
48-57
8


3
LEYDPRLVYD
65-74
9


4
GERTRGRQPG
89-98
10


5
PGDYDDDRRQ
 97-106
11


6
PRREEGGRWG
107-116
12


7
REREEDWRQP
123-132
13


8
EDWRRPSHQQ
134-143
14


9
QPRKIRPEGR
143-152
15


10
TPGQFEDFFP
294-303
16


11
SYLQEFSRNT
311-320
17


12
FNAEFNEIRR
325-334
18


13
EQEERGQRRW
344-353
19


14
DITNPINLRE
393-402
20


15
NNFGKLFEVK
409-418
21


17
RRYTARLKEG
498-507
23


18
ELHLLGFGIN
525-534
24


19
HRIFLAGDKD
539-548
25


20
IDQIEKQAKD
551-560
26


21
KDLAFPGSGE
559-568
27


22
KESHFVSARP
578-587
28









The Ara h 1 IgE binding epitopes are indicated as the single letter amino acid code. The position of each peptide with respect to the Ara h 1 protein (SEQ ID NO. 2) is indicated. The amino acids that, when altered, lead to loss of IgE binding are shown as the bold, underlined residues. Epitopes 16 and 23 were not included in this study because they were recognized by a single patient who was no longer available to the study. All of these sequences can be found in SEQ ID NO. 2.







TABLE 5







Amino acids critical to IgE binding of Ara h 2










EPITOPE
AA SEQUENCE
POSITION
SEQ ID NO.













1
HASARQQWEL
15-24
30


2
QWELQGDRRC
21-30
31


3
DRRCQSQLER
27-36
32


4
LRPCEQHLMQ
39-48
33


5
KIQRDEDSYE
49-58
34


6
YERDPYSPSQ
57-66
35


7
SQDPYSPSPY
65-74
36


8
DRLQGRQQEQ
115-124
37


9
KRELRNLPQQ
127-136
38


10
QRCDLDVESG
143-152
39









The Ara h 2 IgE binding epitopes are indicated as the single letter amino acid code. The position of each peptide with respect to the Ara h 2 protein (SEQ ID NO. 4) is indicated. The amino acids that, when altered, lead to loss of IgE binding are shown as the bold, underlined residues. All of these sequences can be found in SEQ ID NO. 4.







TABLE 6







Amino acids critical to IgE binding of Ara h 3










EPITOPE
AA SEQUENCE
POSITION
SEQ ID NO.













1
IETWNPNNQEFECAG
33-47
40


2
GNIFSGFTPEFLEQA
240-254
41


3
VTVRGGLRILSPDRK
279-293
42


4
DEDEYEYDEEDRRRG
303-317
43









The Ara h 3 IgE binding epitopes are indicated as the single letter amino acid code. The position of each peptide with respect to the Ara h 3 protein (SEQ ID NO. 6) is indicated. The amino acids that, when altered, lead to loss of IgE binding are shown as the bold, underlined. All of these sequences can be found in SEQ ID NO. 6.
Example 7
A Modified Ara H 2 Protein Binds Less IgE but Similar Amounts of IgG
In order to determine the effect of changes to multiple epitopes within the context of the intact allergen, four epitopes (including the three immunodominant epitopes) of the Ara h 2 allergen were mutagenized and the protein produced recombinantly. The amino acids at position 20, 31, 60, and 67 of the Ara h 2 protein (shown in SEQ ID NO. 4) were changed to alanine by mutagenizing the gene encoding this protein by standard techniques. These residues are located in epitopes 1, 3, 6, and 7 and represent amino acids critical to IgE binding that were determined in Example 2. The modified and wild-type versions of this protein were produced and immunoblot analysis performed using serum from peanut sensitive patients. These results showed that the modified version of this allergen bound significantly less IgE than the wild-type version of these recombinant proteins but bound similar amounts of IgG.
Example 8
A Modified Ara H 2 Protein Retains the Ability to Stimulate T Cells to Proliferate
The modified recombinant Ara h 2 protein described in Example 3 was used in T cell proliferation assays to determine if it retained the ability to activate T cells from peanut sensitive individuals. Proliferation assays were performed on T cell lines grown in short-term culture developed from six peanut sensitive patients. T cells lines were stimulated with either 50 μg of crude peanut extract, 10 μg of native Ara h 2, 10 μg of recombinant wild-type Ara h 2, or 10 μg of modified recombinant Ara h 2 protein and the amount of 3H-thymidine determined for each cell line. Results were expressed as the average stimulation index (SI) which reflected the fold increase in 3H-thymidine incorporation exhibited by cells challenged with allergen when compared with media treated controls (FIG. 8).
Example 9
A Modified Ara H 2 Protein Elicits a Smaller Wheal and Flare in Skin Prick Tests of a Peanut Sensitive Individual
The modified recombinant Ara h 2 protein described in Example 3 and the wild-type version of this recombinant protein were used in a skin prick test of a peanut sensitive individual. Ten micrograms of these proteins were applied separately to the forearm of a peanut sensitive individual, the skin pricked with a sterile needle, and 10 minutes later any wheal and flare that developed was measured. The wheal and flare produced by the wild-type Ara h 2 protein (8 mm×7 mm) was approximately twice as large as that produced by the modified Ara h 2 protein (4 mm×3 mm). A control subject (no peanut hypersensitivity) tested with the same proteins had no visible wheal and flare but, as expected, gave positive results when challenged with histamine. In addition, the test subject gave no positive results when tested with PBS alone. These results indicate that an allergen with only 40% of its IgE binding epitopes modified (4/10) can give measurable reduction in reactivity in an in vivo test of a peanut sensitive patient.
These same techniques can be used with the other known peanut allergens, Ara h 1 (SEQ ID NOs. 1 and 2), Ara h 3 (SEQ ID NOs. 5 and 6), or any other allergen.
Modifications and variations of the methods and materials described herein will be obvious to those skilled in the art. Such modifications and variations are intended to come within the scope of the appended claims.











APPENDIX A





ALLERGEN 
SYSTEMATIC AND 


ACCESSION NO. 


SOURCE
ORIGINAL NAMES
MW KDA
SEQ 
OR REFERENCES















WEED POLLENS











Asterales






Ambrosia
Amb a 1; antigen E
38
C
8, 20


artemisiifolia
Amb a 2; antigen K
38
C
8, 21


(short ragweed)
Amb a 3; Ra3
11
C
22



Amb a 5; Ra5
5
C
11, 23



Amb a 6; Ra6
10
C
24, 25



Amb a 7; Ra7
12
P
26



Amb a ?
11
C
27


Ambrosia trifida
Amb t 5; Ra5G
4.4
C
9, 10, 28


(giant ragweed)






Artemisia vulgaris
Art v 1
27-29 
C
28A


(mugwort)
Art v 2
35
P
29


Helianthus annuus
Hel a 1
34
—
29a


(sunflower)
Hel a 2; profilin
15.7
C
Y15210


Mercurialis annua
Mer a 1; profilin
14-15 
C
Y13271







GRASS POLLENS











Poales






Cynodon dactylon
Cyn d 1
32
C
30, S83343


(Bermuda grass)
Cyn d 7

C
31, X91256



Cyn d 12; profilin
14
C
31a, Y08390


Dactylis glomerata
Dac g 1; AgDg1
32
P
32


(orchard grass)
Dac g 2
11
C
33, S45354



Dac g 3

C
33a, U25343



Dac g 5
31
P
34


Holcus lanatus
Hol l 1

C
Z27084, Z68893


(velvet grass)






Lolium perenne
Lol p 1; group I
27
C
35, 36


(rye grass)
Lol p 2; group II
11
C
37, 37a, X73363



Lol p 3; group III
11
C
38



Lol p 5; Lol p IX,
31/35

34, 39



Lol p Ib






Lol p 11; trypsin
16

39a



inh. Related





Phalaris aquatica
Pha a 1

C
40, S80654


(canary grass)






Phleum pratense
Phl p 1
27
C
X78813


(timothy grass)
Phl p 2

C
41, X75925



Phl p 4

P
41A



Phl p 5; Ag25
32
C
42



Phl p 6

C
43, Z27082



Phl p 12; profilin

C
44, X77583



Phl p 13; polygalacturonase
55-60
C
AJ238848


Poa pratensis
Poa p 1; group I
33
P
46


(Kentucky blue
Poa p 5
31/34
C
34, 47


grass)






Sorghum halepense
Sor h 1

C
48


(Johnson grass)











TREE POLLENS











Fagales






Alnus glutinosa
Aln g 1
17
C
S50892


(alder)






Betula verrucosa
Bet v 1
17
C
see list of


(birch)



isoallergens






M65179



Bet v 2; profilin
15
C
X79267



Bet v 3
8
C
X87153/S54819



Bet v 4

C
AF135127



Bet v 5; isoflavone reductase
33.5
C




homologue






Bet v 7; cyclophilin
18
C
P P81531


Carpinus betulus
Car b 1
17
C
51


(hornbeam)






Castanea sativa
Cas s 1; Bet v 1 homologue
22
P
52


(chestnut)
Cas s5; chitinase





Corylus avelana
Cor a 1
17
C
53


(hazel)






Quercus alba
Que a 1
17
P
54


(white oak)






Cryptomeria japonica
Cry j 1
41-45
C
55, 56


(sugi)
Cry j 2

C
57, D29772


Juniperus ashei
Jun a 1
43
P
P81294


(mountain cedar)
Jun a 3
30
P
P81295


Juniperus oxycedrus
Jun o 2; calmodulin-like
29
C
AF031471


(prickly juniper)






Juniperus sabinoides
Jun s 1
50
P
58


(mountain cedar)






Juniperus virginiana
Jun v 1
43
P
P81825


(eastern red cedar)






Oleales






Fraxinus excelsior
Fra e 1
20
P
58A


(ash)






Ligustrum vulgare
Lig v 1
20
P
58A


(privet)






Olea europea
Ole e 1;
16
C
59, 60


(olive)
Ole e 2; profilin
15-18
C
60A



Ole e 3;
9.2

60B



Ole e 4;
32
P
P80741



Ole e 5; superoxide dismutase
16
P
P80740



Ole e 6;
10
C
U86342


Syringa vulgaris
Syr v 1
20
P
58A


(lilac)











MITES











Acarus siro
Aca s 13; fatty acid-bind.prot.
14*
C
AJ006774


(mite)






Blomia tropicalis
Blo t 5;

C
U59102


(mite)
Blo t 12; Bt1 1a

C
U27479



Blo t 13; Bt6 fatty acid-binding prot

C
U58106


Dermatophagoides
Der p 1; antigen P1
25
C
61


pteronyssinus
Der p 2;
14
C
62


(mite)
Der p 3; trypsin
28/30
C
63



Der p 4; amylase
60
C
64



Der p 5;
14
P
65



Der p 6; chymotrypsin
25
C
66



Der p 7;
22-28
C
67



Der p 8; glutathione transferase

P
67A



Der p 9; collagenolytic serine prot.

C
67B



Der p 10; tropomyosin
36

Y14906



Der p 14; apolipophorin like p

C
Epton p.c.


Dermatophagoides
Der m 1;
25
P
68


microceras (mite)






Dermatophagoides
Der f 1;
25
C
69


farinae (mite)
Der f 2;
14
C
70, 71



Der f 3;
30
C
63



Der f 10; tropomyosin

C
72



Der f 11; paramyosin
98
C
72a



Der f 14; Mag3, apolipophorin

C
D17686


Euroglyphus maynei
Eur m 14; apolipophorin
177
C
AF149827


(mite)






Lepidoglyphus
Lep d 2.0101;
15
C
73, 74, 75


destructor
Lep d 2.0102;
15
C
75


(storage mite)











ANIMALS











Bos domesticus
Bos d 2; Ag3, lipocalin
20
C
76, L42867


(domestic cattle)
Bos d 4; alpha-lactalbumin
14.2
C
M18780


(see also foods)
Bos d 5; beta-lactoglobulin
18.3
C
X14712



Bos d 6; serum albumin
67
C
M73993



Bos d 7; immunoglobulin
160

77



Bos d 8; caseins
20-30

77


Canis familiaris
Can f 1;
25
C
78, 79


(Canis domesticus
Can f 2;
27
C
78, 79


(dog)
Can f ?; albumin

C
S72946


Equus caballus
Equ c 1; lipocalin
25
C
U70823


(domestic horse)
Equ c 2; lipocali
18.5
P
79A, 79B


Felis domesticus
Fel d 1; cat-1
38
C
15


(cat saliva)






Mus musculus
Mus m 1; MUP
19
C
80, 81


(mouse urine)






Rattus norvegius
Rat n 1
17
C
82, 83


(rat urine)











FUNGI


Ascomycota











Dothidiales






Alternaria alternata
Alt a 1;
28
C
U82633



Alt a 2;
25
C
U87807, U87808



Alt a 3; heat shock protein
70
C
X78222,



Alt a 6; ribosomal protein
11
C
U87806



Alt a 7; YCP4 protein
22
C
X78225



Alt a 10; aldehyde dehydrogenase
53
C
X78227, P42041



Alt a 11; enolase
45
C
U82437



Alt a 12; acid.ribosomal prot P1
11
C
X84216


Cladosporium
Cla h 1;
13

83a, 83b


herbarum
Cla h 2;
23

83a, 83b



Cla h 3; aldehyde dehydrogenase
53
C
X78228



Cla h 4; ribosomal protein
11
C
X78223



Cla h 5; YCP4 protein
22
C
X78224



Cla h 6; enolase
46
C
X78226



Cla h 12; acid.ribosomal prot P1
11
C
X85180


Eurotiales







Asp fl 13; alkaline
34

84



serine proteinase





Aspergillus
Asp f 1;
18
C
83781,S39330


Fumigatus
Asp f 2;
37
C
U56938



Asp f 3; peroxisomal protein
19
C
U20722



Asp f 4;
30
C
AJ001732



Asp f 5; metalloprotease
42
C
Z30424



Asp f 6; Mn superoxide dismutase
26.5
C
U53561



Asp f 7;
12
C
AJ223315



Asp f 8; ribosomal protein P2
11
C
AJ224333



Asp f 9;
34
C
AJ223327



Asp f 10; aspartic protease
34

X85092



Asp f 11; peptidyl-prolyl isom
24

84a



Asp f 12; heat shock prot. P70
65
C
U92465



Asp f 13; alkaline serine proteinase
34

84b



Asp f 15;
16
C
AJ002026



Asp f 16;
43
C
g3643813



Asp f 17;
34
C
AJ224865



Asp f 18; vacuolar serine
90

84c



Asp f ?;
55
P
85



Asp f ?;

P
86


Aspergillus niger
Asp n 14; beta-xylosidase
105
C
AF108944



Asp n 18;
34
C
84b



vacuolar serine






proteinase






Asp n ?;
85
C
Z84377


Aspergillus oryzae
Asp o 2; TAKA-amylase A
53
C
D00434, M33218



Asp o 13; alkaline serine proteinase 
34
C
X17561


Penicillium
Pen b 13; alkaline serine Proteinase 
33

86a


brevicompactum






Penicillium citrinum
Pen c 1; heat shock protein P70
70
C
U64207



Pen c 3; peroxisomal membrane


86b



protein






Pen c 13; alkaline serine proteinase 
33

86a


Penicillium notatum
Pen n 1; N-acetyl
68

87



glucosaminidase






Pen n 13; alkaline serine proteinase 
34

89



Pen n 18; vacuolar serine proteinase 
32

89


Penicillium oxalicum
Pen o 18; vacuolar serine proteinase 
34

89


Onygenales






Trichophyton rubrum
Tri r 2;

C
90



Tri r 4; serine protease

C
90


Trichophyton
Tri t 1;
30
P
91


tonsurans
Tri t 4; serine protease
83
C
90


Saccharomycetales






Candida albicans
Cand a 1
40
C
88


Candida boidinii
Cand b 2
20
C
J04984, J04985







Basidiomycota











Basidiolelastomycetes






Malassezia furfur
Mal f 1;


91a



Mal f 2; MF1 peroxisomal
21
C
AB011804



membrane protein






Mal f 3; MF2 peroxisomal
20
C
AB011805



membrane protein






Mal f 4,
35
C
Takesako, p.c.



Mal f 5;
18* 
C
AJ011955



Mal f 6; cyclophilin homologue
17* 
C
AJ011956


Basidiomycetes






Psilocybe cubensis
Psi c 1;
16

91b



Psi c 2; cyclophilin





Coprinus comatus
Cop c 1;
11
C
AJ132235


(shaggy cap)
Cop c 2;






Cop c 3;


Brander, p.c.



Cop c 5;


Brander, p.c.



Cop c 7;


Brander, p.c.







INSECTS











Aedes aegyptii
Aed a 1; apyrase
68
C
L12389


(mosquito)
Aed a 2;
37
C
M33157


Apis mellifera
Api m 1; phospholipase A2
16
C
92


(honey bee)
Api m 2; hyaluronidase
44
C
93



Api m 4; melittin
3
C
94



Api m 6;
7-8
P
Kettner, p.c.


Bombus
Bom p 1; phospholipase
16
P
95


pennsylvanicus
Bom p 4; protease

P
95


(bumble bee)






Blattella germanica
Bla g 1; Bd90k

C
96


(German cockroach)
Bla g 2; aspartic protease
36
C




Bla g 4; calycin
21
C
97



Bla g 5; glutathione transf.
22
C
98



Bla g 6; troponin C
27
C
98


Periplaneta americana
Per a 1; Cr-PII
72-78
C
98A


(American cockroach)
Per a 3; Cr-PI

C




Per a 7; tropomyosin
37
C
Y14854


Chironomus thummi
Chi t 1-9; hemoglobin
16
C
99


thummi (midges)
Chi t 1.01; component III
16
C
P02229



Chi t 1.02; component IV
16
C
P02230



Chi t 2.0101; component I
16
C
P02221



Chi t 2.0102; component IA
16
C
P02221



Chi t 3; component II-beta
16
C
P02222



Chi t 4; component IIIA
16
C
P02231



Chi t 5; component VI
16
C
P02224



Chi t 6.01; component VIIA
16
C
P02226



Chi t 6.02; component IX
16
C
P02223



Chi t 7; component VIIB
16
C
P02225



Chi t 8; component VIII
16
C
P02227



Chi t 9; component X
16
C
P02228


Dolichovespula
Dol m 1; phospholipase A1
35
C
100


maculata
Dol m 2; hyaluronidase
44
C
101


(white face hornet)
Dol m 5; antigen 5
23
C
102, 103


Dolichovespula
Dol a 5; antigen 5
23
C
104


arenaria






(yellow hornet)






Polistes annularies
Pol a 1; phospholipase A1
35
P
105


(wasp)
Pol a 2; hyaluronidase
44
P
105



Pol a 5; antigen 5
23
C
104


Polistes dominulus
Pol d 1;
32-34
C
DR Hoffman


(Mediterranean paper
Pol d 4; serine protease


DR Hoffman


wasp)
Pol d 5;


P81656


Polistes exclamans
Pol e 1; phospholipase A1
34
P
107


(wasp)
Pol e 5; antigen 5
23
C
104


Polistes fuscatus
Pol f 5; antigen 5
23
C
106


(wasp)






Polistes metricus
Pol m 5; antigen 5
23
P
106


(wasp)






Vespa crabo
Vesp c 1; phospholipase
34
P
107


(European hornet)
Vesp c 5.0101; antigen 5
23
C
106



Vesp c 5.0102; antigen 5
23
C
106


Vespa mandarina
Vesp m 1.01;


DR Hoffman


(giant asian hornet)
Vesp m 1.02;


DR Hoffman



Vesp m 5;


P81657


Vespula flavopilosa
Ves f 5; antigen 5
23
C
106


(yellowjacket)






Vespula germanica
Ves g 5; antigen 5
23
C
106


(yellowjacket)






Vespula maculifrons
Ves m 1; phospholipase A1
33.5
C
108


(yellowjacket)
Ves m 2; hyaluronidase
44
P
109



Ves m 5; antigen 5
23
23
104


Vespula
Ves p 5; antigen 5
23
C
106


pennsylvanica






(yellowjacket)






Vespula squamosa
Ves s 5; antigen 5
23
C
106


(yellowjacket)






Vespula vidua
Ves vi 5;
23
C
106


(wasp)






Vespula vulgaris
Ves v 1; phopholipase Al
35
C
105A


(yellowjacket)
Ves v 2; hyaluronidase
44
P
105A



Ves v 5; antigen 5
23
C
104


Myrmecia pilosula
Myr p 1,

C
X70256


(Australian jumper ant)
Myr p 2;

C
S81785


Solenopsis geminata
Sol g 2;


DR Hoffman


(tropical fire ant)
Sol g 4


DR Hoffman


Solenopsis invicta
Sol i 2;
13
C
110, 111


(fire ant)
Sol i 3;
24
C
110



Soli 4;
13
C
110


Solenopsis saevissima
Sols 2;


DR Hoffman


(brazilian fire ant)











FOODS











Gadus callarias
Gad c 1; allergen M
12
C
112, 113


(cod)






Salmo salar
Sals 1; parvalbumin
12
C
X97824, X97825


(Atlantic salmon)






Bos domesticus
Bos d 4; alpha-lactalbumin
14.2
C
M18780


(domestic cattle)
Bos d 5; beta-lactoglobulin
18.3
C
X14712



Bos d 6; serum albumin
67
C
M73993



Bos d 7; immunoglobulin
160

77



Bos d 8; caseins
20-30

77


Gallus domesticus
Gal d 1; ovomucoid
28
C
114, 115


(chicken)
Gald 2; ovalbumin
44
C
114, 115



Gald 3; conalbumin (Ag22)
78
C
114, 115



Gald 4; lysozyme
14
C
114, 115



Gal d 5; serum albumin
69
C
X60688


Metapenaeus ensis
Met e 1; tropomyosin

C
U08008


(shrimp)






Penaeus aztecus
Pen a 1; tropomyosin
36
P
116


(shrimp)






Penaeus indicus
Pen i 1; tropomyosin
34
C
117


(shrimp)






Todarodes pacificus
Tod p 1; tropomyosin
38
P
117A


(squid)






Haliotis Midae
Hal m 1
49
—
117B


(abalone)






Apium graveolens
Api g 1; Bet v 1 homologue
 16*
C
Z48967


(celery)
Api g 4; profilin


AF129423



Api g 5;
55/58
P
P81943


Brassica juncea
Bra j 1; 2S albumin
14
C
118


(oriental mustard)






Brassica rapa
Bra r 2; prohevein-like protein
25
?
P81729


(turnip)






Hordeum vulgare
Hor v 1; BMAI-1
15
C
119


(barley)






Zea mays
Zea m 14; lipid transfer prot. 
9
P
P19656


(maize, corn)






Corylus avellana
Cor a 1.0401; Bet v 1
17
C
AF136945


(hazelnut)
homologue





Malus domestica
Mal d 1; Bet v 1 homologue

C
X83672


(apple)
Mal d 3; lipid transfer protein
9
C
Pastorello


Pyrus communis
Pyr c 1; Bet v 1 homologue
18
C
AF05730


(pear)
Pyr c 4; profilin
14
C
AF129424



Pyr c 5; isoflavone reductase
33.5
C
AF071477



homologue





Oryza sativa
Ory s 1;

C
U31771


(rice)






Persea americana
Pers a 1; endochitinase
32
C
Z78202


(avocado)






Prunus armeniaca
Pm ar 1; Bet v 1 homologue

C
U93165


(apricot)
Pm ar 3; lipid transfer protein
9
P



Prunus avium
Pm av 1; Bet v 1 homologue

C
U66076


(sweet cherry)
Pm av 2; thaumatin homologue

C
U32440



Pm av 4; profilin
15
C
AF129425


Prunus persica
Pm p 3;lipid transfer protein
10
P
P81402


(peach)






Sinapis alba
Sin a 1; 2S albumin
14
C
120


(yellow mustard)






Glycine max
Gly m 1.0101; HPS
7.5
P
121


(soybean)
Gly m 1.0102; HPS
7
P
121



Gly m 2
8
P
A57106



Gly m 3; profilin
14
C
AJ223982


Arachis hypogaea
Ara h 1; vicilin
63.5
C
L34402


(peanut)
Ara h 2; conglutin
17
C
L77197



Ara h 3; glycinin
14
C
AF093541



Ara h 4; glycinin
37
C
AF086821



Ara h 5; profilin
15
C
AF059616



Ara h 6; conglutin homolog
15
C
AF092846



Ara h 7; conglutin homolog
15
C
AF091737


Actinidia chinensis
Act c 1; cysteine protease
30
P
P00785


(kiwi)






Solanum tuberosum
Sol t 1; patatin
43
P
P15476


(potato)






Bertholletia excelsa
Ber e 1; 2S albumin
9
C
P04403, M17146


(Brazil nut)






Juglans regia
Jug r 1; 2S albumin
44
C
U66866


(English walnut)
Jug r 2; vicilin

C
AF066055


Ricinus communis
Ric c 1; 2S albumin

C
P01089


(Castor bean)











OTHERS











Anisakis simplex
Ani s 1
24
P
A59069


(nematode)
Ani s 2; paramyosin
97
C
AF173004


Ascaris suum
Asc s 1;
10
P
122


(worm)






Aedes aegyptii
Aed a 1; apyrase
68
C
L12389


(mosquito)
Aed a 2;
37
C
M33157


Hevea brasiliensis
Hev b 1; elongation factor
58
P
123, 124


(rubber)
Hev b 2; ( 1,3-glucanase
58
P
123, 124



Hev b 2; ( 1,3-glucanase
34/36
C
125



Hev b 3
24
P
126, 127



Hev b 4; component of
100/110/115
P
128



microhelix protein complex






Hev b 5
16
C
U42640



Hev b 6.01 hevein precursor
20
C
M36986/p02877



Hev b 6.02 hevein
5
C
M36986/p02877



Hev b 6.03 C-terminal
14
C
M36986/p02877



fragment


U80598



Hev b 7; patatin homologue
46
C
Y15042



Hev b 8; profilin
14
C
AJ132580/AJ1



Hev b 9; enolase
51
C
32581






AJ249148



Hev b 10; Mn-superoxide
26
C




dismut





Ctenocephalides felis
Cte f 1;
—
—
—


felis
Cte f 2; M1b
27
C
AF231352


(cat flea)






Homo sapiens
Hom s 1;
73*
C
Y14314


(human
Hom s 2;
10.3*
C
X80909


autoallergens)
Hom s 3;
20.1*
C
X89985



Hom s 4;
36*
C
Y17711



Hom s 5;
42.6*
C 
P02538









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## Claims

1. A method of treating peanut allergy in a subject susceptible to an anaphylactic allergic response to a peanut allergen protein, the method comprising a step of:
administering to the subject via topical, oral, or local administration, a composition comprising:
dead E. coli cells that have expressed a recombinant version of a modified food allergen protein whose amino acid sequence differs from that of a corresponding wild-type food allergen protein in that at least one IgE epitope has a substitution in the modified food allergen protein such that the modified food allergen protein has a reduced ability to bind to or cross-link IgE as compared with unmodified wild-type food allergen protein, the at least one IgE epitope being one that is recognized when the unmodified wild-type food allergen protein is contacted with serum IgE from an individual that is allergic to the unmodified wild-type food allergen protein, wherein:
when unmodified wild-type Ara h 1 protein has the amino acid sequence of SEQ ID NO: 2, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 25 and 34 of SEQ ID NO: 2;
an epitope found between amino acids 48 and 57 of SEQ ID NO: 2;
an epitope found between amino acids 65 and 74 of SEQ ID NO: 2;
an epitope found between amino acids 89 and 98 of SEQ ID NO: 2;
an epitope found between amino acids 97 and 106 of SEQ ID NO: 2;
an epitope found between amino acids 107 and 116 of SEQ ID NO: 2;
an epitope found between amino acids 123 and 132 of SEQ ID NO: 2;
an epitope found between amino acids 134 and 143 of SEQ ID NO: 2;
an epitope found between amino acids 294 and 303 of SEQ ID NO: 2;
an epitope found between amino acids 311 and 320 of SEQ ID NO: 2;
an epitope found between amino acids 325 and 334 of SEQ ID NO: 2;
an epitope found between amino acids 344 and 353 of SEQ ID NO: 2;
an epitope found between amino acids 393 and 402 of SEQ ID NO: 2;
an epitope found between amino acids 409 and 418 of SEQ ID NO: 2;
an epitope found between amino acids 461 and 470 of SEQ ID NO: 2;
an epitope found between amino acids 498 and 507 of SEQ ID NO: 2;
an epitope found between amino acids 525 and 534 of SEQ ID NO: 2;
an epitope found between amino acids 539 and 548 of SEQ ID NO: 2;
an epitope found between amino acids 551 and 560 of SEQ ID NO: 2;
an epitope found between amino acids 559 and 568 of SEQ ID NO: 2;
an epitope found between amino acids 578 and 587 of SEQ ID NO: 2;
an epitope found between amino acids 597 and 606 of SEQ ID NO: 2;
and a combination thereof; and

when unmodified wild-type Ara h 2 protein has the amino acid sequence of SEQ ID NO: 4, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 15 and 24 of SEQ ID NO: 4;
an epitope found between amino acids 21 and 30 of SEQ ID NO: 4;
an epitope found between amino acids 27 and 36 of SEQ ID NO: 4;
an epitope found between amino acids 39 and 48 of SEQ ID NO: 4;
an epitope found between amino acids 49 and 58 of SEQ ID NO: 4;
an epitope found between amino acids 57 and 66 of SEQ ID NO: 4;
an epitope found between amino acids 65 and 74 of SEQ ID NO: 4;
an epitope found between amino acids 115 and 124 of SEQ ID NO: 4;
an epitope found between amino acids 127 and 136 of SEQ ID NO: 4;
an epitope found between amino acids 143 and 152 of SEQ ID NO: 4;
and a combination thereof; and

when unmodified wild-type Ara h 3 protein has the amino acid sequence of SEQ ID NO: 6, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 33 and 47 of SEQ ID NO: 6;
an epitope found between amino acids 240 and 254 of SEQ ID NO: 6;
an epitope found between amino acids 279 and 293 of SEQ ID NO: 6;
an epitope found between amino acids 303 and 317 of SEQ ID NO: 6;
and a combination thereof:

the expressed recombinant version of the food allergen protein being encapsulated within the dead E. coli cells, so that the encapsulated recombinant version of the food allergen protein is not exposed to the subject's IgE antibodies during administration;
and a pharmaceutically acceptable carrier,

so that one or more symptoms of the subject's allergy to the food allergen protein is reduced or decreased.

2. A method of treating peanut allergy in a subject susceptible to an anaphylactic allergic response to a peanut allergen protein, the method comprising a step of:
administering to the subject via topical, oral, or local administration a composition comprising:
dead E. coli cells that have expressed a non-secreted, recombinant version of a modified food allergen protein whose amino acid sequence differs from that of a corresponding wild-type food allergen protein in that at least one IgE epitope has a substitution in the modified food allergen protein such that the modified food allergen protein has a reduced ability to bind to or cross-link IgE as compared with unmodified wild-type food allergen protein, the at least one IgE epitope being one that is recognized when the unmodified wild-type food allergen protein is contacted with serum IgE from an individual that is allergic to the unmodified wild-type food allergen protein, wherein:
when unmodified wild-type Ara h 1 protein has the amino acid sequence of SEQ ID NO: 2, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 25 and 34 of SEQ ID NO: 2;
an epitope found between amino acids 48 and 57 of SEQ ID NO: 2;
an epitope found between amino acids 65 and 74 of SEQ ID NO: 2;
an epitope found between amino acids 89 and 98 of SEQ ID NO: 2;
an epitope found between amino acids 97 and 106 of SEQ ID NO: 2;
an epitope found between amino acids 107 and 116 of SEQ ID NO: 2;
an epitope found between amino acids 123 and 132 of SEQ ID NO: 2;
an epitope found between amino acids 134 and 143 of SEQ ID NO: 2;
an epitope found between amino acids 294 and 303 of SEQ ID NO: 2;
an epitope found between amino acids 311 and 320 of SEQ ID NO: 2;
an epitope found between amino acids 325 and 334 of SEQ ID NO: 2;
an epitope found between amino acids 344 and 353 of SEQ ID NO: 2;
an epitope found between amino acids 393 and 402 of SEQ ID NO: 2;
an epitope found between amino acids 409 and 418 of SEQ ID NO: 2;
an epitope found between amino acids 461 and 470 of SEQ ID NO: 2;
an epitope found between amino acids 498 and 507 of SEQ ID NO: 2;
an epitope found between amino acids 525 and 534 of SEQ ID NO: 2;
an epitope found between amino acids 539 and 548 of SEQ ID NO: 2;
an epitope found between amino acids 551 and 560 of SEQ ID NO: 2;
an epitope found between amino acids 559 and 568 of SEQ ID NO: 2;
an epitope found between amino acids 578 and 587 of SEQ ID NO: 2;
an epitope found between amino acids 597 and 606 of SEQ ID NO: 2;
and a combination thereof; and

when unmodified wild-type Ara h 2 protein has the amino acid sequence of SEQ ID NO: 4, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 15 and 24 of SEQ ID NO: 4;
an epitope found between amino acids 21 and 30 of SEQ ID NO: 4;
an epitope found between amino acids 27 and 36 of SEQ ID NO: 4;
an epitope found between amino acids 39 and 48 of SEQ ID NO: 4;
an epitope found between amino acids 49 and 58 of SEQ ID NO: 4;
an epitope found between amino acids 57 and 66 of SEQ ID NO: 4;
an epitope found between amino acids 65 and 74 of SEQ ID NO: 4;
an epitope found between amino acids 115 and 124 of SEQ ID NO: 4;
an epitope found between amino acids 127 and 136 of SEQ ID NO: 4;
an epitope found between amino acids 143 and 152 of SEQ ID NO: 4;
and a combination thereof; and

when unmodified wild-type Ara h 3 protein has the amino acid sequence of SEQ ID NO: 6, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 33 and 47 of SEQ ID NO: 6;
an epitope found between amino acids 240 and 254 of SEQ ID NO: 6;
an epitope found between amino acids 279 and 293 of SEQ ID NO: 6;
an epitope found between amino acids 303 and 317 of SEQ ID NO: 6;
and a combination thereof:


the expressed recombinant version of the food allergen protein being encapsulated within the dead E. coli cells; and
a pharmaceutically acceptable carrier,

so that one or more symptoms of the subject's allergy to the food allergen protein is reduced or decreased.

3. A method of treating peanut allergy in a subject susceptible to an anaphylactic allergic response to a food allergen protein, the method comprising a step of:
administering to the subject via topical, oral, or local administration a composition comprising:
dead E. coli cells that have expressed a recombinant version of a modified food allergen protein whose amino acid sequence differs from that of a corresponding wild-type food allergen protein in that at least one IgE epitope has a substitution in the modified food allergen protein such that the modified food allergen protein has a reduced ability to bind to or cross-link IgE as compared with unmodified wild-type food allergen protein, the at least one IgE epitope being one that is recognized when the unmodified wild-type food allergen protein is contacted with serum IgE from an individual that is allergic to the unmodified wild-type food allergen protein, wherein:
when unmodified wild-type Ara h 1 protein has the amino acid sequence of SEQ ID NO: 2, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 25 and 34 of SEQ ID NO: 2;
an epitope found between amino acids 48 and 57 of SEQ ID NO: 2;
an epitope found between amino acids 65 and 74 of SEQ ID NO: 2;
an epitope found between amino acids 89 and 98 of SEQ ID NO: 2;
an epitope found between amino acids 97 and 106 of SEQ ID NO: 2;
an epitope found between amino acids 107 and 116 of SEQ ID NO: 2;
an epitope found between amino acids 123 and 132 of SEQ ID NO: 2;
an epitope found between amino acids 134 and 143 of SEQ ID NO: 2;
an epitope found between amino acids 294 and 303 of SEQ ID NO: 2;
an epitope found between amino acids 311 and 320 of SEQ ID NO: 2;
an epitope found between amino acids 325 and 334 of SEQ ID NO: 2;
an epitope found between amino acids 344 and 353 of SEQ ID NO: 2;
an epitope found between amino acids 393 and 402 of SEQ ID NO: 2;
an epitope found between amino acids 409 and 418 of SEQ ID NO: 2;
an epitope found between amino acids 461 and 470 of SEQ ID NO: 2;
an epitope found between amino acids 498 and 507 of SEQ ID NO: 2;
an epitope found between amino acids 525 and 534 of SEQ ID NO: 2;
an epitope found between amino acids 539 and 548 of SEQ ID NO: 2;
an epitope found between amino acids 551 and 560 of SEQ ID NO: 2;
an epitope found between amino acids 559 and 568 of SEQ ID NO: 2;
an epitope found between amino acids 578 and 587 of SEQ ID NO: 2;
an epitope found between amino acids 597 and 606 of SEQ ID NO: 2;
and a combination thereof; and

when unmodified wild-type Ara h 2 protein has the amino acid sequence of SEQ ID NO: 4, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 15 and 24 of SEQ ID NO: 4;
an epitope found between amino acids 21 and 30 of SEQ ID NO: 4;
an epitope found between amino acids 27 and 36 of SEQ ID NO: 4;
an epitope found between amino acids 39 and 48 of SEQ ID NO: 4;
an epitope found between amino acids 49 and 58 of SEQ ID NO: 4;
an epitope found between amino acids 57 and 66 of SEQ ID NO: 4;
an epitope found between amino acids 65 and 74 of SEQ ID NO: 4;
an epitope found between amino acids 115 and 124 of SEQ ID NO: 4;
an epitope found between amino acids 127 and 136 of SEQ ID NO: 4;
an epitope found between amino acids 143 and 152 of SEQ ID NO: 4;
and a combination thereof; and

when unmodified wild-type Ara h 3 protein has the amino acid sequence of SEQ ID NO: 6, the substitution is in an IgE epitope selected from the group consisting of:
an epitope found between amino acids 33 and 47 of SEQ ID NO: 6;
an epitope found between amino acids 240 and 254 of SEQ ID NO: 6;
an epitope found between amino acids 279 and 293 of SEQ ID NO: 6;
an epitope found between amino acids 303 and 317 of SEQ ID NO: 6;
and a combination thereof:


the expressed recombinant version of the food allergen protein being encapsulated within the dead E. coli cells, wherein the administered dead E. coli are taken up by antigen presenting cells in the subject, the recombinant version of the food allergen protein is released and processed inside the antigen presenting cells, and the processed protein is displayed on the antigen presenting cell surface; and
a pharmaceutically acceptable carrier,

so that one or more symptoms of the subject's allergy to the food allergen protein is reduced or decreased.

4. The method of any one of claims 1, 2, and 3, wherein in the step of administering, the recombinant version of the food allergen protein has a reduced number of IgE binding sites as compared to the unmodified wild-type food allergen protein.

5. The method of any one of claims 1, 2, and 3, wherein the topical administration is selected from the group consisting of nasal, pulmonary, and other mucosal routes.