# Sampling Episode Report

Duke Energy Carolinas’

Allen Steam Station

Belmont, NC

Sampling Episode 6561

Prepared for:



# U.S. Environmental Protection Agency

Engineering and Analysis Division

Office of Water

1200 Pennsylvania Avenue, NW

Washington, D.C. 20460

Prepared by:



# Eastern Research Group, Inc.

14555 Avion Parkway

Suite 200

Chantilly, VA 20151

13 December 2011

EPA Contract No. 68-C-02-095

Work Assignment 9-29

# i CONTENTS

Page

1. I NTRODUCTION 

# ................................................................................................................. 1-1

1.1 Background of Detailed Study ............................................................................. 1-1

1.2 Power Plant Site Selection ................................................................................... 1-1

1.3 Sampling Episode Overview................................................................................ 1-2

2. F ACILITY 

# O VERVIEW 

# ........................................................................................................ 2-1

2.1 FGD Scrubber System ......................................................................................... 2-1

2.2 FGD Wastewater Trea tment System ................................................................... 2-2

2.2.1 Physical/Chemical Separation ................................................................. 2-3

2.2.2 Biological Reactors .................................................................................. 2-4

2.2.3 Sampling Points ....................................................................................... 2-5

3. S AMPLE 

# C OLLECTION 

# ....................................................................................................... 3-9

3.1 Sampling Points, Sample Collection Me thods, and Field Measurements ........... 3-9

3.1.1 Influent to FGD Wastewater Treatment System .................................... 3-10

3.1.2 Influent to Bioreactor System ................................................................ 3-12

3.1.3 Effluent from Bioreactor System ........................................................... 3-14

3.2 List of Analytes and Sample Preservation ......................................................... 3-17

3.3 Plant Operational Data ....................................................................................... 3-18

3.4 Deviations from the Sampling and Analysis Plan ............................................. 3-18

4. L ABORATORY 

# A NALYTICAL 

# R ESULTS AND 

# D ISCUSSION 

# ................................................... 4-1

5. D ATA 

# Q UALITY 

# ................................................................................................................. 5-1

5.1 Analytical Quality Control ................................................................................... 5-1

5.2 Field Quality Control ........................................................................................... 5-1

5.2.1 Bottle Blanks ............................................................................................ 5-1

5.2.2 Equipment Blanks .................................................................................... 5-2

5.2.3 Field Blanks ............................................................................................. 5-2

5.2.4 Field Duplicates ....................................................................................... 5-3

6. R EFERENCES 

# ..................................................................................................................... 6-1

Appendix A: A NALYTICAL 

# R ESULTS



# Appendix B: D ATA 

# R EVIEW 

# N ARRATIVES



# Appendix C: P ICTURES 

# F ROM THE 

# S AMPLING 

# E PISODE

# ii LIST OF TABLES

Page

Table 3-1. Sample Collecti on Information, Allen ..................................................................... 3-20

Table 3-2. Sample Collection at Allen Steam Station ............................................................... 3-22

Table 3-3. Field Measurements, Allen ....................................................................................... 3- 23

Table 3-4. Crosswalk of Sample Number to Sampling Point and Day, Allen ........................... 3-25

Table 3-5. Analytical Methods, Allen ........................................................................................ 3 -26

Table 3-6. Summary of Sample Containe r and Planned Preservation, Allen ............................ 3-27

Table 3-7. Sample Preservation Summary, Allen ...................................................................... 3-28

Table 3-8. Generating Unit Operating Character istics during Sample Collection, Allen ......... 3-31

Table 3-9. FGD Scrubber System Oper ating Characteristics, Allen ......................................... 3-32

Table 3-10. Coal Usage during Sa mple Collection, Allen......................................................... 3-33

Table 3-11. Scrubber Slurry Information during Sample Collection, Allen .............................. 3-34

Table 3-12. FGD Wastewater Treatment System Operation Information, Allen ...................... 3-35

Table 4-1. Influent to FGD Wastew ater Treatment System, Allen ............................................ 4-3

Table 4-2. Influent to the Bioreactor System, Allen ................................................................... 4-6

Table 4-3. Effluent from the Bioreactor System, Allen ............................................................ 4-10

Table 4-4. Classical Polluta nts 4-Day Average Data Summary for FGD Wastewater

Treatment System, Allen ............................................................................................... 4-13

Table 4-5. Metal Pollutants 4-Day Aver age Data Summary fo r FGD Wastewater

Treatment System, Allen ............................................................................................... 4-14

Table 5-1. Field Duplicate Results – Influent to Bior eactor System, Allen ............................... 5-5

Table 5-2. Field Duplicate Results – Effluent from Bi oreactor System, Allen .......................... 5-8

Table A-1. Influent to FGD Wastewater Treatment System, Day 1 Analytical Results,

Allen ......................................................................................................................... ....... A-1

Table A-2. Influent to FGD Wastewater Treatment System, Day 2 Analytical Results,

Allen ......................................................................................................................... ....... A-4

# LIST OF TABLES (Continued)

Page

iii Table A-3. Influent to FGD Wastewater Treatment System, Day 3 Analytical Results,

Allen ......................................................................................................................... ....... A-7

Table A-4. Influent to FGD Wastewater Treatment System, Day 4 Analytical Results,

Allen ......................................................................................................................... ..... A-10

Table A-5. Influent to Bioreactor Syst em, Day 1 Analytical Results, Allen ........................... A-13

Table A-6. Influent to Bioreactor Syst em, Day 2 Analytical Results, Allen ........................... A-16

Table A-7. Influent to Bioreactor Syst em, Day 3 Analytical Results, Allen ........................... A-19

Table A-8. Influent to Bioreactor Syst em, Day 4 Analytical Results, Allen ........................... A-22

Table A-9. Effluent from Bi oreactor System, Day 1 Analyt ical Results, Allen ...................... A-25

Table A-10. Effluent from Bi oreactor System, Day 2 Analyt ical Results, Allen .................... A-28

Table A-11. Effluent from Bi oreactor System, Day 3 Analyt ical Results, Allen .................... A-31

Table A-12. Effluent from Bi oreactor System, Day 4 Analyt ical Results, Allen .................... A-34

Table A-13. Duplicate of Influe nt to Bioreactor System Anal ytical Results, Allen ................ A-37

Table A-14. Duplicate of Effluent from Bior eactor System Analytical Results, Allen .......... A-40

Table A-15. Bottle Blank and Equipment Blank Analytical Data, Allen ................................. A-43

Table A-16. Influent to FGD Wastewater Treatment System Field Blank, Day 1

Analytical Results, Allen .............................................................................................. A-48

Table A-17. Influent to Bioreac tor System Field Blank, Day 1 Analytical Results, Allen ..... A-50

Table A-18. Influent to Bioreac tor System Field Blank, Day 2 Analytical Results, Allen ..... A-52

Table A-19. Influent to Bioreac tor System Field Blank, Day 3 Analytical Results, Allen ..... A-53

Table A-20. Influent to Bioreac tor System Field Blank, Day 4 Analytical Results, Allen ..... A-54

Table A-21. Effluent from Bi oreactor System Field Blank, Day 1 Analytical Results,

Allen ......................................................................................................................... ..... A-55

Table A-22. Effluent from Bi oreactor System Field Blank, Day 2 Analytical Results,

Allen ......................................................................................................................... ..... A-57

Table A-23. Effluent from Bi oreactor System Field Blank, Day 3 Analytical Results,

Allen ......................................................................................................................... ..... A-58

# LIST OF TABLES (Continued)

Page

iv Table A-24. Effluent from Bi oreactor System Field Blank, Day 4 Analytical Results,

Allen ......................................................................................................................... ..... A-59

# v LIST OF FIGURES

Page

Figure 2-1. Simplified Diagram of the FGD Wa stewater Treatment System, Allen ................... 2-6

Figure 2-2. Physical/Chemical Separation and Biological Reactor Portion of FGD

Wastewater Treatment System, Allen .............................................................................. 2-7

Figure C-1. Sampler Working Inside a “Cleanbox” ....................................................................C-1

Figure C-2. Sample Tap and Tubing at the In fluent to the FGD Wastewater Treatment

System ........................................................................................................................ ......C-2

Figure C-3. Sample Collection Set- Up at the Pump-Off Station .................................................C-3

Figure C-4. Sample Collection Set-Up at the Influent to the Bioreactor System ........................C-4

Figure C-5. Sample Tap and Tubing at the E ffluent from the Bioreactor System ......................C-5

# 1-1 1. I NTRODUCTION



# The Engineering and Analysis Division (EAD) of the U.S. Environmental Protection

Agency (EPA) is conducting a sampling program at steam electric power plants. The sampling

program will characterize raw wastewaters generate d by coal-fired steam electric power plants

and evaluate treatment technologi es and best management practic es used to reduce pollutant

discharges. This Sampling Episode Report (SER ) describes the sampling activities that took

place August 1 - 6, 2010, at Duke Energy Carolina s’ Allen Steam Station (Allen), located in

Belmont, North Carolina (Sampling Episode 6561). The focus of Episode 6561 was to

characterize the purge stream entering the flue gas desulfurization (FGD) wastewater treatment

system and the influent to and effluent from the FGD bioreactor treatment system. The influent

to the bioreactor system also represents the e ffluent from the chemical precipitation system.

1.1 Background of Detailed Study

During EPA’s recently completed detailed study of the steam electric industry, EPA

identified metals and certain other constituents pr esent in the wastewater discharges from steam

electric power plants that substantially cont ribute to the toxic load ings for the industry.

Additionally, EPA determined that one of the wa ste streams contributing most of these toxic

loadings are associated with wet flue gas desu lfurization (FGD) systems present at coal-fired

power plants. Based on these findings, EPA ha s focused the sampling program on characterizing

the untreated FGD wastewaters a nd effluent quality from tec hnologies designed to treat the

wastewater.

1.2 Power Plant Site Selection

EPA selected steam electric plants for F GD wastewater sampling based on the process

configurations and characte ristics of the plants.

Allen was selected by EPA for sampling base d on the following site characteristics:

 

# The plant is a coal-fired power plant that burns bituminous coal in each of its five

generating units;

 

# The plant operates two limestone forced oxidation wet FGD systems that service

all five generating units;

 

# The plant operates a segregated FGD wastewater treatment system, which

includes the following steps:

— Equalization,

— pH adjustment (lime addition),

— Ferric chloride addition,

— Polymer addition,

— Clarification,

— Filtration, and

— Biological treatment (nitrogen and metals);

# 1-2  

# The plant operates a selective non-cataly tic reduction (SNCR) system on all five

generating units; and

 

# The plant produces a commerc ial-grade gypsum by-product.

1.3 Sampling Episode Overview

EPA and its technical contractor, Eastern Research Group, Inc. (ERG) conducted a oneday pre-sampling site visit at Allen on Oct ober 22, 2009. The informati on collected during the

pre-sampling site visit and subsequent follow-up telephone calls to Alle n representatives was

used to create Sampling Plan, Duke Energy Carolinas’ Allen Steam Station (Sampling Plan) [1],

which describes the sample collection pro cedures used during the sampling episode.

On Sunday, August 1, 2010, EPA arrived at Allen to meet with plant personnel, receive

on-site safety training, review the sampling poi nts, and determine the set-up for the sample

collection. EPA and ERG collected the sample s specified in the Sampling Plan on Monday

through Friday (August 2 – 6, 2010). The following personnel participated in EPA’s sample

collection activities:

 

# Ron Jordan, EPA/EAD;

 

# Bill Swietlik, EPA/EAD;

 

# TJ Finseth, ERG;

 

# Kavya Kasturi, ERG;

 

# Lydia Lambert, ERG;

 

# Jennifer Lewis, ERG; and

 

# Jessica Wolford, ERG.

In addition, during the sample collection, EPA collected split sample s with the Utility

Water Act Group (UWAG). UWAG is a trade asso ciation for the electric power generating

industry. During the sampling epis ode, UWAG acted as Allen’s representative and collected

samples on behalf of the plant.

# 2-1 2. F ACILITY 

# O VERVIEW



# Allen operates five steam electric units and each has a coal-fired boiler. Units 1 and 2

came on line in 1957, and each has a capacity of 165 megawatts (MW). Units 3, 4, and 5 came

on line in 1959, 1960, and 1961, respectively, and each has a capacity of 275 MW. All five

generating units burn bituminous coal. The electr ic generating units at Allen are operated on a

cycling basis and are not baseload units. The pl ant is located on 1,000 acres adjacent to the

Catawba River.

Allen recently retrofitted all five units with two FGD scr ubber systems (Absorbers 1 and

3) to control the sulfur dioxide (SO 2 

# ) releases from the plant. Units 1, 2, and 5 exhaust to FGD

Absorber 1 (which began operation in Februa ry 2009), and Units 3 and 4 exhaust to FGD

Absorber 3 (which began operation in May 2009). The wet FGD scrubbe rs are designed to

remove more than 95 percent of SO 2 

# from the flue gas. With the recent addition of the FGD

scrubber systems, Allen also installed an FGD wastewater treatment system to control the

discharges of the wastewater generated during the operation of the FGD scrubbers. Sections 2.1

and 2.2 further discuss the FGD scrubber system s and the FGD wastewater treatment system,

respectively.

Allen operates electrostatic precipitators (ESPs) to control fly ash and particulate

emissions from the plant. Allen also opera tes SNCR systems year-round to control nitrogen

oxide (NO

x 

# ) emissions from the plant. Additionall y, the boilers are operated with low-NO

x 

# burners and a separated overfire air system.

The ESPs at Allen are particulate contro l devices that are de signed to remove 99.9

percent of the fly ash and other particulates from the flue gas. Allen utili zes ESPs to remove the

fly ash from all five generating units. The fly as h and other particulates are collected in hoppers

and usually pneumatically transferred to fly ash s ilos. However, Allen also has the capability of

handling the fly ash wet by sluicing the fly as h in the hoppers and tr ansferring the fly ash

transport water to the ash basin. Allen uses the wet fly ash handling system during start ups for

the generating units until the units are burning 100 percent coal. Allen also uses the wet fly ash

handling system if there are issues wi th the dry fly ash handling system.

All five generating units have the following configuration:

 

# Boiler (SNCR system contained in boiler);

 

# Air preheater;

 

# ESP; and

 

# FGD scrubber system.

2.1 FGD Scrubber System

Allen operates two wet FGD scrubber systems that treat the flue gas from all five electric

generating units. The plant uses limestone slurry as the sorbent for su lfur dioxide absorption.

Limestone and service water are fed to on-site ba ll mills, which pulverize the limestone to create

the slurry. From the ball mills, the slurry is tr ansferred to hydrocyclones to collect limestone

particles that are too la rge for use in the FGD system. The underflow from the hydrocyclones is

transferred to the reject tank and then sent back to the ball mill for additional processing. The

# 2-2 overflow from the hydrocyclones, which is approxima tely 30 percent solids, is transferred to the

reagent tank for storage and then used as fresh limestone slurry in the absorber.

The fresh limestone slurry is fed to the r eaction tank of the FGD system and the recycle

pumps send the slurry to the various levels of th e FGD scrubber. Both absorbers have four spray

levels; however, the plant typically operates onl y three levels at one time. The slurry is

pressurized and sprayed downward into the scru bber vessel through spray nozzles. As the slurry

falls downward and the flue gas flows countercurr ent to the slurry, the spray droplets of the

limestone slurry contact the flue gas and absorb the SO 2 

# , which reacts with the limestone

particles and produces calcium sulfite. The spra y droplets continue to flow downward in the

scrubber until they reach the reaction tank. Alle n bubbles air through the reaction tank to oxidize

the calcium sulfite to calci um sulfate (i.e., gypsum).

Allen intermittently blows down the gypsum slu rry from the reaction tank to control the

solids and/or chlorides concentration in the sc rubbers. The chlorides concentration in the

scrubbers is maintained between 3,000 and 10,000 ppm (parts per million), with a design

maximum chlorides concentration of 12,000 ppm. Th e typical percentage of solids within the

scrubber is 14 to 16 percent.

The gypsum slurry is pumped to the prim ary hydrocyclones where the solid gypsum is

separated from the water by centrifugal force. The solid gypsum is forced outward to the walls of

the hydrocyclones and falls downward, while the wa ter exits the top of the hydrocyclones. The

gypsum from the primary hydrocyclones is transf erred to the filter feed tank, which is then

transferred to the vacuum filter belts. Th e overflow from the primary hydrocyclones is

transferred to a holding tank and then pumped to the secondary hydrocyclones. The underflow

from the secondary hydrocyclones is returned to the scrubber. The overflow from the secondary

hydrocyclones is sent to the FGD wastewater treatment system. Th is FGD purge stream contains

0.5 to 0.8 percent solids.

Allen washes the gypsum sent to the vacuum filt er belts with service water as it is applied

to the belts to reduce the chlorides in the gyps um to typically less th an 20 ppm. The vacuum

filter belts dry the gypsum to a moisture conten t of approximately four percent. The gypsum

produced at the plant must have a chloride cont ent of less than 120 ppm and a moisture content

of less than 10 percent to be so ld to a wallboard manufacturer. Allen transfers the dry gypsum to

a storage area and then trucks the gypsum to a wallboard manuf acturer in Mount Holly, North

Carolina.

2.2 FGD Wastewater Treatment System

The FGD wastewater treatment system is de signed to receive intermittent transfers of

FGD wastewater from the FGD sy stem, which consists of seconda ry hydrocyclone overflow, at a

flow rate of approximately 400 gallons per minut e (gpm). The FGD wastew ater treatment system

consists of chemical precipitation and biologi cal treatment technologies. Allen installed the

wastewater treatment system to treat the FGD scrubber purge wa stewater to meet its permit

limits, which include limits for total suspe nded solids (TSS) and selenium. The Allen FGD

wastewater treatment system contains the following treatment operations:

# 2-3  

# Physical/Chemical Separation:

— Equalization,

— pH adjustment (lime addition),

— Ferric chloride addition,

— Polymer addition,

— Clarification, and

— Filtration; and

 

# Biological Reactors:

— Cooling (heat extraction),

— Nutrient addition,

— First-stage biol ogical reaction,

— Nutrient addition, and

— Second-stage biolog ical reaction.

Figures 2-1 and 2-2, located at the end of this section, present proces s flow diagrams of

the FGD wastewater treatment system, as well as the sampling locations for EPA’s sampling

episode. Figure 2-1 is a simplified diagram of the FGD wastewater trea tment system, showing

different portions of the system. Figure 2-2 is a diagram of the chemical precipitation and

biological reactor po rtions of the treatment system.

The secondary hydrocyclone overflow from the scrubbers is transfe rred to the purge tank

and then sent to the equalization tank at the start of the wastewat er treatment syst em. Allen also

has a maintenance tank that is used as either a spare equalization tank or a spare sludge holding

tank. During the sampling episode, the FGD influe nt wastewater was found to contain less than

0.5 percent solids, had a pH between 6.5 and 7.0 S. U., and a temperature between 40 and 50°C.

The equalization tank also receives water from the filtrate sump (which includes filter press

filtrate), gravity filter backwash reject, backfl ush waste from the biological treatment system,

and off-specification biologi cal treatment effluent.

2.2.1 Physical/Chemical Separation

From the equalization tank, the wastewater enters three reac tion tanks in series: reaction

tank 1, in which lime is added to increase th e pH to between 8.6 and 9.2 S.U.; reaction tank 2,

formerly used for organosulfide addition, but wh ich is now used to pr ovide additional mixing;

and reaction tank 3, in which ferric chloride is added.

The overflow from reaction tank 3 flows through a flow splitter, which distributes the

flow evenly to two clarifiers. Allen operates the two clarifiers in parallel, each capable of

handling 100 percent of the waste stream flow. A polymer is added to the wastewater just before

it enters the clarifiers. Hydrochl oric acid is added to the wast ewater at a point between the

clarifiers and the sand filters to ensure that the pH is adjusted closer to neutral prior to the

biological treatment system. Af ter the hydrochloric acid addition, the overflow from the two

clarifiers is split and sent th rough three sand filters in parallel, each of which can handle 75

percent of the waste stream capac ity. The sand filters are continuous backwash gravity filters and

produce an effluent with a TSS concentration of approximately 1 to 2 ppm. The filter backwash

is sent to a sump and then tran sferred to the equalization tank.

# 2-4 The sludge collected in the clarifier is tran sferred to a sludge hol ding tank. A portion of

the clarifier sludge is recycled from the sludge ho lding tank to reaction tank 1, while the majority

is pumped to a filter press for dewatering. The solids are transferred to an off-site landfill and the

filtrate is recycled to the equalization tank.

2.2.2 Biological Reactors

Prior to entering the biological portion of the wastewater treatment system, the

wastewater is cooled if necessary by heat exchan gers to 95o F, the optimal temperature for the

biological treatment system. The he at exchangers were constructed with a titanium alloy that can

withstand the high levels of ch lorides in the FGD wastewater entering the biological treatment

system. When in use, the heat exchanger requires 300 to 350 gpm of cooling water. Allen adds

nutrients (in the form of molasses) to the wastewater as it flows from the heat exchanger to the

biological treatment system. The nutrients prov ide a source of carbon to feed the bacteria.

In the bioreactor portion of th e treatment, Allen treats the wa stewater in two stages. Each

stage contains eight bioreactor cells, which are oper ated in parallel. Each cell contains 20 feet of

activated carbon media. Within the activated carbon bed, specialized microbes fill the voided

areas and reduce selenium to its elemental form, while other metals are precipitated as sulfide

complexes. The microbes also reduce the concentr ation of nitrogen present in the wastewater.

The wastewater is divided evenly among the first stage of eight cells. The flow to each cell is

sent to 16 distribution pipes to create an even di stribution of flow into the top of the cell. The

water level in the cell is designe d to be approximately six inches above the activated carbon bed;

however, at the time of the site visit, the wa ter level was below the top of the carbon bed.

Allen operates the bioreactors to achieve a negative oxidation re duction potential (ORP)

within the system, which provides the optimum e nvironment to reduce selenium to its elemental

form. This ORP is controlled by the amount of nutr ient that is fed to the system. The plant also

controls the pH in the bioreac tor cells to between 6.0 and 9.0 S.U. because extreme high or low

pH levels could affect the performance of the microbes a nd potentially allow nondesirable

microbes to propagate.

The effluent from all eight of the first-stage bi oreactor cells is combined in the first-stage

effluent storage area. The wastewater is then transferred to the eight second-stage bioreactor

cells, which are operated in parallel. Nutrients are added to the wastewater as feed for the

microbes as the wastewater is tran sferred. As in the first stage, the wastewater is divided evenly

among the eight second-stage cells and each cell has 16 distributor pi pes to evenly distribute the

flow into the cell.

Occasionally the bioreactor cells must be b ackflushed to remove accumulated solids. The

plant pumps the effluent from the first-stage bi oreactor cells backward s through each cell and it

overflows into a spillover chamber. The plan t then returns the backwash overflow to the

equalization tank at the start of th e FGD wastewater treatment system.

Because the first-stage bioreac tor cells denitrify the waste stream, they produce nitrogen

and carbon dioxide gas. To remove the gas from the cells, the plant oc casionally performs a

degassing operation where water is sent backward s through the cells, like a backflush, but the

flush is only long enough for the gas to “burp” out of the system. The second-stage bioreactor

# 2-5 cells are primarily anoxic and do not produce as much gas as the first-stage cells, requiring less

frequent degassing than the first-stage cells..

From the second stage of the bioreactor trea tment system, the wastew ater is commingled

with the heat exchanger cooling water and transfe rred to the ash basin. The effluent from the ash

basin is discharged to the Catawba River via Outfall 002.

Because the steam electric generating units at Allen are operated on a cycling basis and

are not baseload units, there are times when the units are shut down for a few days. During these

shut-down periods, the wastewat er treatment system does not receive any scrubber purge

wastewater and, therefore, the plan t has to find a way to feed the microbes to keep them alive. If

Allen knows ahead of time that all units will be shut down for a couple of days, they can build up

additional scrubber purge in the equalization a nd maintenance tanks and then operate the FGD

wastewater treatment system at a reduced flow rate until they begin opera ting again. If the plant

is down for several days without advanced know ledge, the plant can run service water through

the system and feed nutrients to the bi oreactors to sustain biological growth.

2.2.3 Sampling Points

Figure 2-2 presents the sampling points for the Allen sampling episode; they are

summarized below:

 

# A sample of the purge stream entering the FGD wastewater treatment system

downstream of the purge tank and upstr eam of the equalization tank (i.e., the

sample was collected prior to any chem ical addition or mixing with recycle

streams);

 

# An in-process sample of the influent to the bioreactor system, downstream of the

sand filters and the holding tank and upstream of the heat exchanger; and

 

# A sample of the effluent from the bi oreactor system, downstream of the secondstage effluent storage area and upstream of where the FGD wastewater treatment

effluent is commingled with the heat exchanger cooling water.

# 2-6Figure 2-1. Simplified Diagram of theFGD Wastewater Treatment System, Allen

# 2-7Figure 2-2. Physical/ChemicalSeparation and Biological Reactor Portionof FGD Wastewater Treatment System, Allen

# 2-8[QUESTION FOR ALLEN: Please confirm that the sampling points and the flow path for theheat exchanger cooling water are depictedaccurately on the process flow diagram.]

# 3-9 3. S AMPLE 

# C OLLECTION



# During the Allen sampling episode, the samp ling team followed the collection protocols

specified in the Sampling Plan. Section 3.1 desc ribes the sampling points, sample collection

methods, and field measurements. Section 3.2 desc ribes the list of analytes and the sample

preservation procedures. Secti on 3.3 discusses the plan t operational data collected during the

sampling episode. Finally, Section 3.4 discusse s any deviations from the planned sampling

activities.

3.1 Sampling Points, Sample Collectio n Methods, and Field Measurements

This section describes the sampling point s, sample collection methods, and field

measurements. Additional details are available in Section 3 of the Sampling Plan. Figures 2-1

and 2-2 of this report present flow diagrams of the plant’s wastewater treatment system,

including sampling point locations.

Table 3-1 lists the sampling points, sampli ng point descriptions, type of sample

collection, and sample collection dates and times. Table 3-2 list s the sampling point numbers and

descriptions, the number of samples collected, and the pollutant parameters for which the

samples were analyzed. Table 3-3 presents th e field measurement data collected from each

sampling point. Table 3-4 presents a crosswalk be tween the sample numbers used for laboratory

sample identification and the sample points for each day.

The sampling points for this episode were:

 

# SP-1 Influent to FGD Wastewater Treatment System;

 

# SP-2 Influent to Bioreactor System;

 

# SP-3 Effluent from Bioreactor System;

 

# SP-4 Duplicate of Influent to Bioreactor System (SP-2);

 

# SP-5 Duplicate of Effluent fr om Bioreactor System (SP-3);

 

# SP-6 Influent to FGD Wastewater Treat ment System (SP-1) Field Blank;

 

# SP-7 Influent to Bioreactor Syst em (SP-2) Field Blank; and

 

# SP-8 Effluent from Bioreactor System (SP-3) Field Blank.

For all the sampling points, EPA collected sp lit samples with Allen’s representative by

inserting a “Y” splitter into the sample tubing configuration at each sampling point to allow for

split sample collection. EPA collected all sample s (other than mercury and cyanide) at all

sampling points as “true” splits (i.e., samp les for the same analyte were collected

simultaneously). The mercury and cyanide samples were collected directly from a sample tap;

therefore, for these samples, EPA and Allen’s re presentative collected duplicate samples, with

one filling the bottle immediately after the other.

At each of the sampling points, EPA collected metals sample fractions following EPA

Method 1669 collection protocols. To collect these samples, EPA samplers donned Tyvek®

coveralls and dust masks (dust masks were worn until all mercury samples had been collected).

In addition, EPA set up a “cleanbox,” which consists of a portable plastic rectangular box with a

shallow lip and a flip-top lid th at secures itself ope n with clips. The “cleanbox” is double-bagged

with a clear inner bag, enclosed in a translu cent outer bag. The “cleanbo x” is handled using

“clean hands / dirty hands” techniques, where by the inner bag is ha ndled only by the “clean

# 3-10 hands” sampler. When the “cleanbox” is opened, the sampler is able to position his/her hands in

the box and pull the bag over the open ing of the box, but is still able to see the sample bottles and

tubing. The “cleanbox” minimizes potential at mospheric contamination during sample

collection. Figure C-1, in Appendix C, is a pictur e of a sampler working inside a “cleanbox.”

The metals sample bottles were also double-bagged. To collect the metals samples, EPA

placed the sample bottles (while still in its seal ed inner bag) into the “cleanbox” following the

protocols specified in Method 1669, also referred to as “clean hands / dirty hands” protocols.

Sampling staff filled the sample container, then cl osed the sample bottle lid and sealed the inner

bag as quickly as possible with in the “cleanbox.” The bagged sample bottles were then removed

from the “cleanbox” and placed into the outer bag following the “clean hands / dirty hands”

protocols.

3.1.1 Influent to FGD Wastewater Treatment System

The influent to FGD wastewater treatment syst em (SP-1) was collected from a sample tap

located on the wastewater piping downstream of the purge tank and upstream of the equalization

tank. Figure 2-2 identifies the sampling location for SP-1. This sample was collected as a grab

sample because, according to plant representatives, the wastewater characteristics of the purge to

the wastewater treatment system are fairly cons istent during the purge. In addition, due to the

relatively high concentration of suspended solids , the FGD purge was not amenable to collecting

representative split samples usi ng composite sampling techniques.

The sampling point was located outside, near the equalization tank. The sampling team

set up a tent for the sample collection to protect the samplers from the heat. There was no rain

during the sample collection. The sampling team did note, however, that on Day 1, there was a

slight breeze during sample collection and on Da y 4, there was some gypsum dust in the air near

the sampling point due to truck traffic.

On Day 1 only, prior to collecting the sample s at the influent to the FGD wastewater

treatment system (SP-1), the sampling team collected field blanks (SP-6) for total mercury, total

and dissolved metals, and dissolved hexavalent chromium. See Section 5 .2.3 for a description of

the field blank sample collection procedures at the influent to the FGD wastewater treatment

system.

To collect the wastewater samples at the influent to the FGD wastewater treatment

system, the samplers attached a 10-foot length of silicone tubing to the sample tap on the

wastewater piping. The end of th e silicone tubing was fitted w ith a “Y” splitter, with two

additional lengths of silicone tubi ng attached to the “Y” splitter. Figure C-2, in Appendix C, is a

picture of the tubing attached to the sample tap at the influent to the FGD wastewater treatment

system. The samplers attached the silicone tubi ng to the sample tap, opened the valve, and

allowed sample to flow through the tubing for two minutes before beginning to fill sample

containers. The samplers did not close the valv e until all samples were collected (except the

dissolved metals samples).

The samplers collected the following samples using a “cleanbox,” as described in Section

3.1:

 

# Total and dissolved mercury;

# 3-11  

# Total metals by 200.8; and

 

# Total metals by 200.7.

Samplers then filled a 10-liter container with sample to be pumped off later for collection

of dissolved metals sample fractions. After all samples were collected at the sampling point for

the day, the filled 10-liter container was transported to the staging area, put on ice, and then left

untouched to allow the solids in the wastewater to settle.

The sampling team collected an aliquot of sample for field testing. Sample pH and

temperature were measured immediately at the sa mpling point using four-color pH paper, a pH

meter, and a thermometer. Table 3-3 presents the field measurement data for the influent to the

FGD wastewater treatment system.

The samplers then filled containe rs for biochemical oxygen demand (BOD 5 

# ), Group I,

Group II, and total cyanide samples directly fr om the tubing without using a “cleanbox.” See

Table 3-6 for a list of Group I and Group II analytes. BOD 5 

# , Group I, Group II, and total cyanide

samples were immediately placed on ice after sa mple collection.

The total cyanide sample was immediately preserved. Lead acetate test strips and

potassium-iodide starch test stri ps were used to measure for su lfides and oxidants, respectively.

No sulfides or oxidants were detected during th e sampling episode. The samplers then preserved

the total cyanide sample with sodium hydroxide to a pH >10 S.U. and placed it on ice.

Samples for the dissolved metals analyses we re pumped from the previously filled 10-

liter container. The pump-off station consisted of a length of Teflon® tubing attached to a length

of silicone tubing, which was threaded through an ISCO peristaltic pump. The sampling team

attached a capsule filter to th e silicone tubing, which was a s hort distance downstream of the

pump. They then attached another short length of silicone tubing to the capsule filter effluent,

which was attached to a “Y” spli tter and two lengths of silicone tubing. This set-up allowed EPA

to collect “true” split samples with Allen’s representatives. Fi gure C-3, in Appendix C, is a

picture of the set-up at the pump-off station.

The sampling team placed the end of the Teflon® tubing into the 10-lit er container filled

with wastewater from the influent to the FGD wastewater treatment system. By this time, the

solids in the wastewater had b een settling for approximately 70 minutes. Approximately 0.5- 1

liters of sample were pumped through the tubi ng/filter to purge the line before collecting

samples.

Samplers collected the following dissolved sa mples using a “cleanbox,” as described in

Section 3.1:

 

# Dissolved metals by 200.8;

 

# Dissolved metals by 200.7; and

 

# Dissolved hexavalent chromium.

Hexavalent chromium samples were immedi ately placed on ice after sample collection.

# 3-12 3.1.2 Influent to Bioreactor System

The influent to the bioreactor system (SP-2) was collected downstream of the sand filters

after the wastewater from the th ree filters is commingled. This sample tap is located downstream

of the holding tank and upstream of the heat exchanger. Allen installed a three-way splitter on

the sample tap, which had three separate taps to collect samples from the sampling point. The

sample tap was located inside the FGD wastewat er treatment building (see Figure 2-2 for the

sampling location for SP-2). Because the sampling point was located inside, there were no wind

or rain concerns during the sample collection.

ERG collected the influent from the biorea ctor system composite samples using two

automatic compositors which were programmed to collect individual samp le volumes into two

20-liter containers. The compositors were progr ammed to collect 830 mL every hour during the

24-hour composite period. Following the composite period, ERG pumped off the specific sample

volumes into the individual sample containers.

ERG used one automatic compositor to collect sample volume for the metals analyses

(excluding mercury) and another automatic compos itor to collect sample volume for all classical

analytes (i.e., BOD 5 

# , Group I, and Group II, excluding cy anide). In addition to collecting

automatic composite samples for the analytes listed above, ERG collected total and dissolved

mercury grab samples every six hours during ea ch compositing period. ERG also collected one

grab sample for cyanide during each compositing period.

On day 1 only, ERG collected field blank samp les (SP-7) for hexavalent chromium, total

and dissolved metals by 200.7, and total and di ssolved metals by 200.8. See Section 5.2.3 for a

description of the field blank sa mple collection procedures at th e influent to the bioreactor

system.

Samplers set up the compositors by attaching tw o T-splitter tubing sets to two of the taps

on the three-way splitter. The T-splitter tubing set consisted of three silicone tubes attached to a

T-splitter. The tubing configura tion was set up such that one e nd was connected to the sample

tap, one end was threaded through the automatic compositor, and connected to the large

composite container, and one end was placed next to a sump/drain near the sampling point. The

samplers attached the silicone tubing to the samp le tap, opened the valve, and allowed sample to

flow through both sets of tubing before calibrati ng either of the automatic compositors. This

ensured that the flow through the tubing during calibration would be the expected flow through

the tubing during sample collection. The automatic compositors were calibrated such that they

collected 830 mL every hour, so that the project ed complete volume would be 20 liters. After

calibration, the tubing was attached to a vented cap on a 20-liter composite container. The

sample tap was left open during the entire sample collection time period a nd flowed directly to

the sump/drain. The automatic compositor pulled sa mple from the flowing stream as dictated by

the sampling program and was capable of purging th e line back to the flowing stream between

each individual sample aliquot.

Both 20-liter composite containers were placed into plastic trash cans, and maintained on

ice for the entire 24-hour compositing period. A plas tic bag was placed on top of the trash cans

to minimize contamination. Figure C-4, in Appendix C, is a picture of the composite sampling

set-up at the influent to the bioreactor system.

# 3-13 Immediately after setting up the compositors, ERG collected the first set of the total and

dissolved mercury grab samples. At the first gr ab sample every day, ERG collected field blanks

for total mercury samples. See Section 5.2.3 for a de scription of the field blank sample collection

procedures at the influent to the bioreactor system.

For mercury collection, samplers shrouded one of the three taps on the three-way splitter

(the other two taps were being used by the two compositors) with a clean plastic bag by

puncturing a hole in the bottom of the bag with the end of the tap and then securing it to the tap

with a ziptie. After pur ging the tap for two minutes, the samples were collected directly from the

tap into the sample bottles. Becau se total mercury and dissolved mercury samples were collected

directly from the tap, ERG was not able to coll ect split samples with Allen’s representatives;

therefore, duplicate samples were collected. ER G collected another set of total and dissolved

mercury samples every six hours thereafter over the 24-hour period. The plastic shroud was

replaced for each set of mercury grab samples. After collecting each set of mercury grabs, ERG

filled a field test bottle and tested for pH and temperature using four-colored pH paper and a

thermometer and meter. Table 3-3 presents the field measurement data for the influent to the

bioreactor system.

After the collection of the thir d set of mercury grabs, ERG collected a total cyanide grab

sample from the same tap on the three-way splitt er that was used for mercury sample collection.

Because total cyanide samples were collected directly from the ta p, ERG was not able to collect

split samples with Allen’s representatives; theref ore, duplicate samples were collected. The total

cyanide sample was immediately preserved. Lead acetate test strips and potassium-iodide starch

test strips were used to measure for sulfides and oxidants, respectively. No sulfides or oxidants

were detected during the sampling episode at the influent to the bi oreactor system. ERG

samplers then preserved the total cyanide sample s with sodium hydroxide to a pH >10 S.U. and

placed the samples on ice.

After the twenty-fourth aliquots were coll ected in the two composite containers, ERG

followed “clean hands / dirty hands” sampling tec hniques to disassemble the compositors from

the sampling set up. Closed caps were placed on the two composite containers and the

composite containers were moved to the pump-off area where they remained on ice until

individual sample bottles were filled. The samplers then began setting up the compositors for the

next day of samples using new tubing sets and composite containers. The automatic compositor

was programmed to collect the same volum e on each day of sample collection.

After completing the set up for the next day of composite samples, the sampling team

transferred the wastewater coll ected in the 20-liter containe rs into the individual sample

containers at the pump-off area. For this samp ling point, ERG used a sample pump-off approach

to transfer the composite samples to the individual sample bottles. For this sample pump-off

procedure, the dissolved metals samples were collected before the total metals and classical

samples to prevent cross-contamination with in the tubing, which could lead to greater

concentrations in the dissolved metals fractions.

Samples for the dissolved and total metals analyses were pumped from one of the

previously filled 20-liter cont ainers. The pump-off station cons isted of a length of Teflon®

tubing attached to a length of silicone tubing, which was threaded throu gh an ISCO peristaltic

pump. The sampling team attached a capsule fi lter to the silicone t ubing, which was a short

distance downstream of the pump. They then attached another short length of silicone tubing to

# 3-14 the capsule filter effluent, which was attached to a “Y” splitter and two lengths of silicone

tubing. This set-up allowed EPA to collect “tru e” split samples with A llen’s representative.

Figure C-3, in Appendix C, is a picture of the set-up at the pump-off station.

On Day 1 only, prior to collec ting any samples of the influent to the bioreactor system,

the samplers collected a field blank sample (SP- 7) for dissolved and total metals. See Section

5.2.3 for a description of the dissolved metals field blank sample collection at the influent to the

bioreactor system.

After collecting the field blank samples, the sampling team placed the end of the Teflon®

tubing into one of the 20-liter composite containers filled with wastewater from the influent to

the bioreactor system. After the tubing and filter we re purged with two liters of sample, samplers

collected the following samples using a “cl eanbox,” as described in Section 3.1:

 

# Dissolved metals by 200.8;

 

# Dissolved metals by 200.7; and

 

# Dissolved hexavalent chromium.

Hexavalent chromium samples were immediately placed on ice after sample

collection. After the dissolved metals had b een collected, the filter was removed. Once the

tubing was purged with two liters of sample, sa mplers collected the following samples using a

“cleanbox,” as described in Section 3.1:

 

# Total metals by 200.8; and

 

# Total metals by 200.7.

After the metal samples had been collected, the sampling team placed the end of the

Teflon® tubing into the other pr eviously filled 20-liter cont ainer. Once the tubing was purged

with 1-2 liters of sample, samplers fille d containers for biochemical oxygen demand (BOD 5 

# ),

Group I, and Group II samples directly from the tubing without using a “cleanbox.” See Table 3-

6 for a list of Group I and Group II analytes. BOD 5 

# , Group I, and Group II samples were

immediately placed on ice af ter sample collection.

For all analytes, ERG collected QC samples at the influent to the bi oreactor system (SP2) on Day 4 of the sampling episode. In additi on, for all analytes, ERG collected a duplicate

sample of the influent from bioreactor system (SP-4) on Day 3 of the sampling episode.

3.1.3 Effluent from Bioreactor System

The effluent from bioreactor system (SP-3) was collected downstream of the secondstage effluent storage tank from the second-stag e bioreactor cells. Alle n installed a three-way

splitter on the sample tap that had three separate taps to collect samples from the sampling point.

The sample tap was located inside the FGD wastewater treatment building (see Figure 2-2 for the

sampling location for SP-3). Because the sampling point was located inside, there were no wind

or rain concerns during the sample collection.

ERG collected the effluent from the biorea ctor system composite samples using two

automatic compositors which were programmed to collect individual samp le volumes into two

20-liter containers. The compositors were progr ammed to collect 830 mL every hour during the

# 3-15 24-hour composite period. Following the composite period, ERG pumped off the specific sample

volumes into the individual sample containers.

ERG used one automatic compositor to collect sample volume for the metals analyses

(excluding mercury) and another automatic compos itor to collect sample volume for all classical

analytes (i.e., BOD 5 

# , Group I, and Group II, excluding cy anide). In addition to collecting

automatic composite samples for the analytes listed above, ERG collected total and dissolved

mercury grab samples every six hours during ea ch compositing period. ERG also collected one

grab sample for cyanide during each compositing period.

On day 1 only, ERG collected field blank samp les (SP-8) for hexavalent chromium, total

and dissolved metals by 200.7, and total and di ssolved metals by 200.8. See Section 5.2.3 for a

description of the field blank sa mple collection procedures at th e effluent from the bioreactor

system.

Samplers set up the compositors by attaching tw o T-splitter tubing sets to two of the taps

on the three-way splitter. The T-splitter tubing set consisted of three silicone tubes attached to a

T-splitter. The tubing configura tion was set up such that one e nd was connected to the sample

tap, one end was threaded through the automatic compositor, and connected to the large

composite container, and one end was placed next to a sump/drain near the sampling point. The

samplers attached the silicone tubing to the samp le tap, opened the valve, and allowed sample to

flow through both sets of tubing before calibrati ng either of the automatic compositors. This

ensured that the flow through the tubing during calibration would be the expected flow through

the tubing during sample collection. The automatic compositors were calibrated such that they

collected 830 mL every hour, so that the project ed complete volume would be 20 liters. After

calibration, the tubing was attached to a vented cap on a 20-liter composite container. The

sample tap was left open during the entire sample collection time period a nd flowed directly to

the sump/drain. The automatic compositor pulled sa mple from the flowing stream as dictated by

the sampling program and was capable of purging th e line back to the flowing stream between

each individual sample aliquot.

Both 20-liter composite containers were placed into plastic trash cans, and maintained on

ice for the entire 24-hour compositing period. A plas tic bag was placed on t op of the trash cans

to minimize contamination. Figure C-5, in Appendi x C, is a picture of the composite sampling

set-up at the effluent from the bioreactor system.

Immediately after setting up the compositors, ERG collected the first set of the total and

dissolved mercury grab samples. At the first gr ab sample every day, ERG collected field blanks

for total mercury samples. See Section 5.2.3 for a de scription of the field blank sample collection

procedures at the effluent fr om the bioreactor system.

For mercury collection, samplers shrouded one of the three taps on the three-way splitter

(the other two taps were being used by the two compositors) with a clean plastic bag by

puncturing a hole in the bottom of the bag with the end of the tap and then securing it to the tap

with a ziptie. After purging the ta p for two minutes, the samples were collected directly from the

tap into the sample bottles. Becau se total mercury and dissolved mercury samples were collected

directly from the tap, ERG was not able to coll ect split samples with Allen’s representatives;

therefore, duplicate samples were collected. ER G collected another set of total and dissolved

mercury samples every six hours thereafter over the 24-hour period. The plastic shroud was

# 3-16 replaced for each set of mercury grab samples. After collecting each set of mercury grabs, ERG

filled a field test bottle and tested for pH and temperature using four-colored pH paper, a pH

meter, and a thermometer. Table 3-3 presents th e field measurement data for the effluent from

the bioreactor system.

After the collection of the thir d set of mercury grabs, ERG collected a total cyanide grab

sample from the same tap on the three-way splitt er that was used for mercury sample collection.

Because total cyanide samples were collected directly from the ta p, ERG was not able to collect

split samples with Allen’s representatives; theref ore, duplicate samples were collected. The total

cyanide sample was immediately preserved. Lead acetate test strips and potassium-iodide starch

test strips were used to measure for sulfides and oxidants, respectively. No sulfides or oxidants

were detected during this sampling episode at the effluent from the bioreactor system. ERG

samplers then preserved the total cyanide sample s with sodium hydroxide to a pH >10 S.U. and

placed the samples on ice.

After the twenty-fourth aliquots were coll ected in the two composite containers, ERG

followed “clean hands / dirty hands” sampling tec hniques to disassemble the compositors from

the sampling set up. Closed caps were placed on the two composite containers and the

composite containers were moved to the pump-off area where they remained on ice until

individual sample bottles were filled. The samplers then began setting up the compositors for the

next day of samples using new tubing sets and composite containers. The automatic compositor

was programmed to collect the same volum e on each day of sample collection.

After completing the set up for the next day of composite samples, the sampling team

transferred the wastewater coll ected in the 20-liter containe rs into the individual sample

containers at the pump-off area. For this samp ling point, ERG used a sample pump-off approach

to transfer the composite samples to the individual sample bottles. For this sample pump-off

procedure, the dissolved metals samples were collected before the total metals and classical

samples to prevent cross-contamination with in the tubing, which could lead to greater

concentrations in the dissolved metals fractions.

Samples for the dissolved and total metals analyses were pumped from one of the

previously filled 20-liter cont ainers. The pump-off station cons isted of a length of Teflon®

tubing attached to a length of silicone tubing, which was threaded throu gh an ISCO peristaltic

pump. The sampling team attached a capsule fi lter to the silicone t ubing, which was a short

distance downstream of the pump. They then attached another short length of silicone tubing to

the capsule filter effluent, which was attached to a “Y” splitter and two lengths of silicone

tubing. This set-up allowed EPA to collect “tru e” split samples with A llen’s representatives.

Figure C-3, in Appendix C, is a picture of the set-up at the pump-off station.

On Day 1 only, prior to collec ting any samples of the effluent from the bioreactor system,

the samplers collected a field blank sample (SP- 8) for dissolved and total metals. See Section

5.2.3 for a description of the dissolved metals fiel d blank sample collection at the effluent from

the bioreactor system.

After collecting the field blank samples, the sampling team placed the end of the Teflon®

tubing into one of the 20-liter composite containers filled with wastewater from the effluent from

the bioreactor system. After the tubing and filter were purged with 1-2 liters of sample, samplers

collected the following samples using a “cl eanbox,” as described in Section 3.1:

# 3-17  

# Dissolved metals by 200.8;

 

# Dissolved metals by 200.7; and

 

# Dissolved hexavalent chromium.

Hexavalent chromium samples were immedi ately placed on ice after sample collection.

After the dissolved metals had been collected, the filter was removed. Once the tubing was

purged with 1-2 liters of sample, samplers coll ected the following samples using a “cleanbox,” as

described in Section 3.1:

 

# Total metals by 200.8; and

 

# Total metals by 200.7.

After the metal samples had been collected, the sampling team placed the end of the

Teflon® tubing into the other prev iously filled 20-liter contai ner. After the tubing was purged

with 1-2 liters of sample, samplers fille d containers for biochemical oxygen demand (BOD 5 

# ),

Group I, and Group II samples directly from the tubing without using a “cleanbox.” See Table 3-

6 for a list of Group I and Group II analytes. BOD 5 

# , Group I, and Group II samples were

immediately placed on ice af ter sample collection.

For all analytes, ERG collected QC samples at the effluent from th e bioreactor system

(SP-3) on Day 3 of the sampling episode. In add ition, for all analytes, ER G collected a duplicate

sample of the effluent from bioreactor syst em (SP-5) on Day 2 of the sampling episode.

3.2 List of Analytes and Sample Preservation

Samples collected at Allen were an alyzed for the following pollutants:

 

# Classicals:

— Biochemical oxygen demand, 5-day (BOD5),

— Chemical oxygen demand (COD),

— Total suspended solids (TSS),

— Total dissolved solids (TDS),

— Sulfate,

— Chloride,

— Total Kjeldahl nitrogen (TKN),

— Ammonia as nitrogen,

— Nitrate/nitrite as nitrogen,

— Total phosphorus,

— Total cyanide;

 

# Total Metals:

— Aluminum, antimony, arsenic, barium, beryllium, boron, cadmium,

calcium, chromium, cobalt, copper , iron, lead, magnesium, manganese,

mercury, molybdenum, nickel, selenium, silver, sodium, thallium, tin,

titanium, vanadium, and zinc.

 

# Dissolved Metals:

— Same parameters as listed for total metals, plus hexavalent chromium.

# 3-18 The analytes selected reflect the current understanding of coal-fired power plant air

pollution control wastewaters, in cluding contributions from coal , scrubber sorb ents, treatment

chemicals, and other sources. Table 3-5 lists the analytical methods used to determine pollutant

concentrations.

Table 3-6 lists the containers used for sample collection as well as the preservation

requirements for each sample. Table 3-7 summarizes the chemical preservation for each of the

samples that were preserved on site. The sampli ng team preserved samples in accordance with

procedures described in Section 3.4 of the Sampling Plan. Immediately following sample

collection, the samplers placed the BOD 5 

# , Group I, Group II, dissolved hexavalent chromium,

and total cyanide samples on ice to cool the sa mples to <6°C. The sampling team did not place

the total and dissolved metals or the total and dissolved mercury samples on ice, which comports

with the requirements of Methods 200.7, 200.8, and 1631E. All dissolved metals samples, except

for mercury, were filtered in the field. The hexavalent chromium samples were preserved in the

field, but all other metals samples were acid-pre served prior to extraction at the laboratory. The

hexavalent chromium samples were preserve d with ammonium sulfate buffer solution and

ammonium hydroxide to a pH between 9.3 and 9.5 S.U. The Group II samples were preserved

with sulfuric acid to a pH of <2 S.U. in the staging area, and the pH was confirmed with fourcolor pH strips. Each total cyanide sample was te sted for sulfides and oxidants immediately after

sample collection. Because sulfides and oxidants we re not detected at any of the sampling points

during the sampling episode, the samplers did not need to dilute the samples or add sodium

arsenite to the samples. After testing for sulf ides and oxidants, the total cyanide samples were

preserved with sodium hydroxide to a pH >10 S.U. and placed on ice.

3.3 Plant Operational Data

During the EPA sampling episode, Allen pl ant personnel provided the sampling team

with operational data for each of the generating units and the F GD scrubber system, as well as

operational and flow data for the FGD wastewater treatment system. Tabl e 3-8 presents the unit

operating characteristics, Table 3-9 presents th e FGD scrubber system operating characteristics,

Table 3-10 presents information on the coal burned during the sampling episode, Table 3-11

presents information on the FGD scrubber slu rry generation during th e sampling episode, and

Table 3-12 presents the FGD wastewater tr eatment system operating characteristics.

3.4 Deviations from the Sampling and Analysis Plan

The sampling episode proceeded as specified in the Sampling Plan with the exceptions

described below:

 

# Preservation of hexavalent chromium: EPA planned to preserve the hexavalent

chromium samples to a pH between 9.3 and 9.7 S.U. using an ammonium sulfate

buffer solution; however, since the amm onium sulfate buffer solution was not

achieving the desired pH, the samplers added only 100 drops of ammonium

sulfate buffer solution, followed by as mu ch ammonium hydroxide as necessary

to reach the desired pH.

 

# Collection of composite metals and cl assicals samples at SP-2 (Day 4): On

day 4 of sample collection at the influent to the bioreactor system (SP-2), the

power failed resulting in missing sample al iquot number 14 for both the classicals

# 3-19 and metals compositors. Both compositors collected sample aliquot 13 as

scheduled at 20:59 for the metals and 21:01 for the classicals. The power failed at

21:44 and was not restored until 23:28, at which time sample aliquot 15 was

collected by the metals compositor followed immediately by the classicals

compositor. Sample aliquot 16 was collect ed as scheduled at 23:59 for the metals

and 0:01 for the classicals.

 

# Collection of composite metals and cl assicals samples at SP-3 (Day 4): On day

4 of sample collection at the effluent of the bioreactor system (SP-3), the power

failed resulting in sample aliquot number 16 not being taken at the predetermined

time. Both compositors collected sample aliquot 15 as scheduled, at 21:30 for

metals and 21:32 for classicals. The powe r failed at 21:45 and was not restored

until 23:26, at which time sample al iquot 16 was collected by the metals

compositor followed immediately by the classicals compositor. Sample aliquot

17 was collected as scheduled at 23:30 fo r the metals and 23:32 for the classicals.

 

# Collection of total mercury sample at SP-1 (Day 4): On day 4 of sample

collection at the influent to the F GD wastewater system (SP-1), Allen’s

representatives collected the total metals volumes before collecting total and

dissolved mercury samples. Allen’s re presentatives were collecting metals

samples while ERG was collecting the total and dissolved mercury samples and

vice versa.

# 3-20 Table 3-1. Sample Collection Information, Allen

Sampling

Point

Number Sampling Point Description Type of

Sample Sample

Collection Date Sample Collection Time

SP-1 Influent to FGD Wastewater Treatment

System – Day 1 Grab 8/2/10 1022 a 

# and 1148 b



# Influent to FGD Wa stewater Treatment

System – Day 2 Grab 8/3/10 1136 a 

# and 1312 b



# Influent to FGD Wa stewater Treatment

System – Day 3 Grab 8/4/10 1127 a 

# and 1311 b



# Influent to FGD Wa stewater Treatment

System – Day 4 Grab 8/5/10 1139 a 

# and 1323 b



# SP-2 Influent to Bioreactor System – Day 1 Composite 8/2/10

8/3/10 0900 c



# 1040 d



# Grab 8/2/10

8/3/10 0913 e 

# , 1519 f 

# , and 2121 g



# 0319 h



# Influent to Bioreactor System – Day 2 Composite 8/3/10

8/4/10 0901 c



# 1031 d



# Grab 8/3/10

8/4/10 0915 e 

# , 1516 f 

# , and 2119 g



# 0319 h



# Influent to Bioreactor System – Day 3 Composite 8/4/10

8/5/10 0900 c



# 1017 d



# Grab 8/4/10

8/5/10 0915 e 

# , 1519 f 

# , and 2121 g



# 0319 h



# Influent to Bioreactor System – Day 4 Composite 8/5/10

8/6/10 0900 c



# 0827 d



# Grab 8/5/10

8/6/10 0914 e 

# , 1517 f 

# , and 2118 g



# 0323 h



# SP-3 Effluent from Bioreactor System – Day 1 Composite 8/2/10

8/3/10 0730 c



# 0952 d



# Grab 8/2/10

8/3/10 0742 e 

# , 1349 f 

# , and 1948 g



# 0149 h



# Effluent from Bioreactor System – Day 2 Composite 8/3/10

8/4/10 0729 c



# 0945 d



# Grab 8/3/10

8/4/10 0745 e 

# , 1351 f 

# , and 1951 g



# 0149 h



# Effluent from Bioreactor System – Day 3 Composite 8/4/10

8/5/10 0731 c



# 0941 d



# Grab 8/4/10

8/5/10 0744 e 

# , 1349 f 

# , and 1953 g



# 0150 h



# Effluent from Bioreactor System – Day 4 Composite 8/5/10

8/6/10 0730 c



# 0655 d



# Grab 8/5/10

8/6/10 0742 e 

# , 1346 f 

# , and 1946 g



# 0146 h



# SP-4 Duplicate of Influent to Bioreactor System

– Day 3 Composite 8/4/10

8/5/10 0900 c



# 1018 d

# 3-21 Table 3-1. Sample Collection Information, Allen

Sampling

Point

Number Sampling Point Description Type of

Sample Sample

Collection Date Sample Collection Time

Grab 8/4/10

8/5/10 0916 e 

# , 1520 f 

# , and 2123 g



# 0321 h



# SP-5 Duplicate of Effl uent from Bioreactor

System – Day 2 Composite 8/3/10

8/4/10 0729 c



# 0945 d



# Grab 8/3/10

8/4/10 0745 e 

# , 1351 f 

# , and 1951 g



# 0149 h



# SP-6 Influent to FGD Wastewater Treatment

System Field Blank Grab 8/2/10 1006 i 

# and 1141 j



# SP-7 Influent to Bioreact or System Field Blank

– Day 1 Composite 8/2/10

8/3/10 0810 k



# 1028 l



# Grab 8/2/10 0907 m



# Influent to Bioreactor System Field Blank

– Day 2 Grab 8/3/10 0909 m



# Influent to Bioreactor System Field Blank

– Day 3 Grab 8/4/10 0910 m



# Influent to Bioreactor System Field Blank

– Day 4 Grab 8/5/10 0908 m



# SP-8 Effluent from Bi oreactor System Field

Blank – Day 1 Composite 8/2/10

8/3/10 0649 k



# 0940 l



# Grab 8/2/10 0737 m



# Effluent from Bior eactor System Field

Blank – Day 2 Grab 8/3/10 0738 m



# Effluent from Bior eactor System Field

Blank – Day 3 Grab 8/4/10 0740 m



# Effluent from Bior eactor System Field

Blank – Day 4 Grab 8/5/10 0737 m

a 

# Start time for the collection of the total metals samples (total metals by 200.7 and total metals by 200.8), total and

dissolved mercury, and classicals (BOD 5 

# , Group I, Group II, and total cyanide).

b 

# Start time for the collection of the dissolved metals samples (dissolved metals by 200.7, dissolved metals by 200.8,

and dissolved hexavalent chromium). Does not include dissolved mercury.

c 

# Started metals compositor. The classical compositor wa s started approximately two minutes after the metals

compositor.

d 

# Start time for the collection of dissolved metals samples (dissolved metals by 200.7, dissolved metals by 200.8,

and dissolved hexavalent chromium), total metals samples (total metals by 200.7 and total metals by 200.8), and

classicals (BOD 5 

# , Group I, and Group II).

e 

# Start time for the collection of the total and dissolved mercury Grab 1 samples.

f 

# Start time for the collection of the total and dissolved mercury Grab 2 samples.

g 

# Start time for the collection of the total and dissolved mercury Grab 3 and total cyanide samples.

h 

# Start time for the collection of the total and dissolved mercury Grab 4 samples.

i 

# Start time for the collection of total mercury, total metals by 200.7, and total metals by 200.8 field blank samples.

j 

# Start time for the collection of dissolved metals by 200.7, dissolved metals by 200.8, and hexavalent chromium

field blank samples.

k 

# Filled 20-liter field blank composite container with blank water.

l 

# Start time for the collection of total metals (total metals by 200.7 and total metals by 200.8) and dissolved metals

(dissolved metals by 200.7, dissolved metals by 200.8, and hexavalent chromium) field blank samples.

m



# Start time for the collection of total mercury field blank samples.3-22Table 3-2. Sample Collection at Allen Steam StationSampling PointNumberSampling Point Name Total Metals (EPA Method 200.8 with collision cell) Total Metals (EPA Method 200.7) Dissolved Metals (EPA Method 200.8 with collision cell) Dissolved Metals (EPA Method 200.7) Total Mercury Dissolved Mercury Hexavalent Chromium BOD 5 Group I

a Group II

b Total CyanideSP-1 Influent to FGD Wastewater Treatment System 4 4 4 4 4 4 4 4 4 4 4SP-2 Influent to Bioreactor System4+1QC 4+1QC 4+1QC4+1QC 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC4+1QCSP-3 Effluent from Bioreactor System 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC 4+1QC4+1QCSP-4 Duplicate of Influent to Bioreactor System 1 1 1 1 1 1 1 1 1 1 1SP-5 Duplicate of Effluent from Bioreactor System 1 1 1 1 1 1 1 1 1 11SP-6 Influent to FGD Wastewater Treatment SystemField Blank1 1 1 1 1 1SP-7 Influent to Bioreactor SystemField Blank 1 1 1 1 4 1SP-8 Effluent from Bioreactor System Field Blank 1 1 1 1 4 1Total Number of Samples 17+2QC17+2QC 17+2QC 17+2QC23+2QC 14+2QC 17+2QC 14+2QC 14+2QC 14+2QC 14+2QCaGroup I includes total suspended solids (TSS), total dissolved solids (TDS), sulfate, and chloride.bGroup II includes ammonia as nitrogen, nitrate/nitrite as nitrogen, total Kjeldahlnitrogen (TKN), chemical oxygen demand (COD), and total phosphorus.3-23Table 3-3. Field Measurements, AllenSamplingPointNumberSampling Point DescriptionDateTimeTemperature (°C)pHWasteStream FlowRate (GPM)NotesThermometerProbeMeteraStripsSP-1 Influent to FGD WastewaterTreatment System – Day 18/2/1010344441.56.87 383bNo detected sulfides or oxidants intotal cyanide grab.Influent to FGD WastewaterTreatment System – Day 28/3/10 115044.548.56.727 375cInfluent to FGD WastewaterTreatment System – Day 38/4/10 114444476.777 374dInfluent to FGD WastewaterTreatment System – Day 48/5/10 115944.547.06.727 377eSP-2 Influent toBioreactor System– Day 18/2/10 091738366.887 297No detected sulfides or oxidants intotal cyanide grab.8/2/10 15223937.46.907 3118/2/10 21253836.66.957 3048/3/10 03243836.96.797 299Influent to Bioreactor System– Day 28/3/10 092536.9386.997 2878/3/10 152037.9396.747 3348/3/10 212237.9396.697 2818/4/10 032537.6396.667 285Influent to Bioreactor System– Day 38/4/10 092038396.607 2928/4/10 152538.639.56.587 3138/4/10 212938.739.56.90NA 2458/5/10 032637.739.56.62NA 233Influent to Bioreactor System– Day 48/5/10 091739NA6.65NA 2008/5/10 15203938.66.576 2008/5/10 21223836.76.886 2218/6/10 032638.537.26.966 2213-24Table 3-3. Field Measurements, AllenSamplingPointNumberSampling Point DescriptionDateTimeTemperature (°C)pHWasteStream FlowRate (GPM)NotesThermometerProbeMeteraStripsSP-3 Effluent from BioreactorSystem – Day 18/2/10 07453331.86.887 324On day 4, it was noted that the 3rdgrab sample taken on 8/5/10 at1950 contained suspended whiteparticles. The particles appeared tobe fibrous in nature and measured 1to 3 mm in length No detectedsulfides or oxidants in total cyanidegrab.8/2/10 13523332.66.807 3678/2/10 19543332.66.797 2838/3/10 01523332.36.827 345Effluent from BioreactorSystem – Day 28/3/10 075232.133.56.767 2678/3/10 135533.3346.667 3448/3/10 195833.3346.687 2928/4/10 015632.8346.727 210Effluent from BioreactorSystem – Day 38/4/10 074932.8346.828 2368/4/10 135533.734.56.737 3398/4/10 195733.834.56.71NA 3158/5/10 015333.4346.78NA 226Effluent from BioreactorSystem – Day 48/5/10 074334NA6.8NA 1908/5/10 13503534.26.866 2278/5/10 19503433.26.837 2418/6/10 015033.532.86.827 225aThe pH meter was calibrated prior to the sampling episode and once every day during the sampling episode using prepackaged buffer solutions at pH 4.0, 7.0, and 10.0.bBased on average of flow meter measurements collected every minute from 1022 to 1036 on 8/2/10 (time of sample collection).cBased on average of flow meter measurements collected every minute from1136 to 1149 on 8/3/10 (time of sample collection).dBased on average of flow meter measurements collected every minute from 1127 to 1143 on 8/4/10 (time of sample collection).eBased on average of flow meter measurements collected every minute from 1139 to 1159 on 8/5/10 (time of sample collection).NA – Not analyzed.

# 3-25 Table 3-4. Crosswalk of Sample Numb er to Sampling Point and Day, Allen

Sample Number Sample Point Sample Description

71791 SP-1, Day 1 Influent to FGD Wastewater Treatment System - Day 1

71792 SP-1, Day 2 Influent to FGD Wastewater Treatment System - Day 2

71793 SP-1, Day 3 Influent to FGD Wastewater Treatment System - Day 3

71794 SP-1, Day 4 Influent to FGD Wastewater Treatment System - Day 4

71795 SP-2, Day 1 Influent to Bioreactor System - Day 1

71796 SP-2, Day 2 Influent to Bioreactor System - Day 2

71797 SP-2, Day 3 Influent to Bioreactor System - Day 3

71798 SP-2, Day 4 Influent to Bioreactor System - Day 4

71799 SP-3, Day 1 Effluent from Bioreactor System - Day 1

71800 SP-3, Day 2 Effluent from Bioreactor System - Day 2

71801 SP-3, Day 3 Effluent from Bioreactor System - Day 3

71802 SP-3, Day 4 Effluent from Bioreactor System - Day 4

71803 SP-4, Day 3 Duplicate of Influent to Bioreactor System - Day 3

71804 SP-5, Day 2 Duplicate of Efflue nt from Bioreactor System - Day 2

71805 SP-6, Day 1 Influent to FGD Wastewat er Treatment System Field Blank - Day 1

71806 SP-7, Day 1 Influent to Bior eactor System Fiel d Blank - Day 1

71807 SP-7, Day 2 Influent to Bior eactor System Fiel d Blank - Day 2

71808 SP-7, Day 3 Influent to Bior eactor System Fiel d Blank - Day 3

71809 SP-7, Day 4 Influent to Bior eactor System Fiel d Blank - Day 4

71810 SP-8, Day 1 Effluent from Bi oreactor System Field Blank - Day 1

71811 SP-8, Day 2 Effluent from Bi oreactor System Field Blank - Day 2

71812 SP-8, Day 3 Effluent from Bi oreactor System Field Blank - Day 3

71813 SP-8, Day 4 Effluent from Bi oreactor System Field Blank - Day 4

# 3-26 Table 3-5. Analytical Methods, Allen

Parameter Method Number

Classicals

Biochemical oxygen demand (BOD 5 

# ) SM 5210 B

Chemical oxygen demand (COD) EPA 410.4

Total suspended solids (TSS) SM 2540 D

Total dissolved solids (TDS) SM 2540 C

Sulfate EPA 300.0

Chloride EPA 300.0

Ammonia as nitrogen EPA 350.1

Nitrate/nitrate as nitrogen EPA 353.2

Total Kjeldahl nitrogen (TKN) EPA 351.2

Total phosphorus EPA 365.1

Total cyanide SM 4500 CN E

Total and Dissolved Metals

Mercury EPA 1631E

Hexavalent chromium (dissolved only) EPA 218.6

Antimony, arsenic, cadmium, chromium, copper, lead, manganese,

nickel, selenium, silver, thallium, vanadium, and zinc EPA 200.8 with collision cell

Aluminum, barium, beryllium, boron, calcium, cobalt, iron, magnesium,

molybdenum, sodium, tin, and titanium EPA 200.7

# 3-27 Table 3-6. Summary of Sample Contai ner and Planned Preservation, Allen

Parameter Sample

Container and

Volume QC Sample

Container and

Volume On-Site Preservation

Classicals

BOD 5 

# One 1-L plastic

bottle One 1-L plastic

bottle ≤ 6  C

Group I a 

# One 1-L plastic

bottles One 1-L plastic

bottles ≤ 6  C

Group II b 

# Two 1-L plastic

bottles Two 1-L plastic

bottles H 2 

# SO 4 

# to pH <2 S.U., ≤ 6  C

Total cyanide One 1-L plastic

bottle One 1-L plastic

bottles Dilution until sulfide is not detected,

NaAsO 2 

# until total chlorine is not

present, NaOH to pH >10 S.U.,

≤ 6  C

Metals

Total metals (EPA Method 200.8 with

collision cell) One 500-mL

plastic bottle No additional

volume needed None

(acid preserve at laboratory)

Total metals (EPA Method 200.7) One 500-mL

plastic bottle No additional

volume needed None

(acid preserve at laboratory)

Dissolved metals (EPA Method 200.8

with collision cell) One 500-mL

plastic bottle No additional

volume needed 0.45-μm filter (performed in field)

(acid preserve at laboratory)

Dissolved metals (EPA Method 200.7) One 500-mL

plastic bottle No additional

volume needed 0.45-μm filter (performed in field)

(acid preserve at laboratory)

Total mercury: None

(acid preserve at laboratory) Manual composite grab samples Four 125-mL

glass bottle No additional

volume needed

Influent samples and field blank

samples Two 250-mL

glass bottle No additional

volume needed

Dissolved mercury: None

(filter and acid preserve at

laboratory) Manual composite grab samples Four 125-mL

glass bottle No additional

volume needed

Influent samples One 250-mL

glass bottle No additional

volume needed

Hexavalent chromium One 250-mL

plastic bottle No additional

volume needed 0.45-μm filter (performed in field)

Ammonium sulfate buffer to

pH 9.3 – 9.7 S.U., ≤ 6  C

(a) Group I includes TSS, TDS, sulfate, and chloride.

(b) Group II includes ammonia as nitrogen, COD, nitr ate/nitrite as nitrogen, TKN, and total phosphorus.

# 3-28 Table 3-7. Sample Preservation Summary, Allen

Sampling Point

Number Analysis Date Chemical

Added Initial pH Final pH Amount

Added

(drops)

71791 Group II Volume 1 8/2/10 H 2 

# SO 4 

# 7 2 28

71791 Group II Volume 2 8/2/10 H 2 

# SO 4 

# 7 2 28

71791 Hexavalent Chromium 8/2/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6.0 8.3 100

NH 4 

# OH 8.3 9.5 8

71805 Hexavalent Chromium 8/2/10 (NH 4 

# ) 2 

# SO 4



# Buffer 8.7 8.7 100

NH 4 

# OH 8.7 9.5 6

71792 Total Cyanide 8/2/10 NaOH 7 10 9

71795 Total Cyanide 8/2/10 NaOH 7 10 10

71799 Total Cyanide 8/2/10 NaOH 7 10 10

71792 Group II Volume 1 8/3/10 H 2 

# SO 4 

# 7 2 24

71792 Group II Volume 2 8/3/10 H 2 

# SO 4 

# 7 2 24

71795 Group II Volume 1 8/3/10 H 2 

# SO 4 

# 6 2 22

71795 Group II Volume 2 8/3/10 H 2 

# SO 4 

# 6 2 22

71799 Group II Volume 1 8/3/10 H 2 

# SO 4 

# 6 2 30

71799 Group II Volume 2 8/3/10 H 2 

# SO 4 

# 6 2 30

71792 Hexavalent Chromium 8/3/10 (NH 4 

# ) 2 

# SO 4



# Buffer Not

Measured 8.5 100

NH 4 

# OH 8.5 9.5 12

71795 Hexavalent Chromium 8/3/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.5 100

NH 4 

# OH 8.5 9.5 10

71799 Hexavalent Chromium 8/3/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.7 100

NH 4 

# OH 8.7 9.5 8

71806 Hexavalent Chromium 8/3/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.5 100

NH 4 

# OH 8.5 9.5 8

71810 Hexavalent Chromium 8/3/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.7 100

NH 4 

# OH 8.7 9.5 8

71792 Total Cyanide 8/3/10 NaOH 7 10 10

71796 Total Cyanide 8/3/10 NaOH 7 10 10

71800 Total Cyanide 8/3/10 NaOH 7 10 15

71804 Total Cyanide 8/3/10 NaOH 7 10 15

71793 Group II Volume 1 8/4/10 H 2 

# SO 4 

# 7 2 28

71793 Group II Volume 2 8/4/10 H 2 

# SO 4 

# 7 2 28

# 3-29 Table 3-7. Sample Preservation Summary, Allen

Sampling Point

Number Analysis Date Chemical

Added Initial pH Final pH Amount

Added

(drops)

71796 Group II Volume 1 8/4/10 H 2 

# SO 4 

# 6 2 28

71796 Group II Volume 2 8/4/10 H 2 

# SO 4 

# 6 2 28

71800 Group II Volume 1 8/4/10 H 2 

# SO 4 

# 7 2 24

71800 Group II Volume 2 8/4/10 H 2 

# SO 4 

# 7 2 24

71804 Group II Volume 1 8/4/10 H 2 

# SO 4 

# 7 2 24

71804 Group II Volume 2 8/4/10 H 2 

# SO 4 

# 7 2 24

71793 Hexavalent Chromium-QC 8/4/10 (NH 4 

# ) 2 

# SO 4



# Buffer Not

Measured 8.1 100

NH 4 

# OH 8.1 9.5 12

71796 Hexavalent Chromium 8/4/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.5 100

NH 4 

# OH 8.5 9.5 10

71800 Hexavalent Chromium 8/4/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.5 100

NH 4 

# OH 8.5 9.5 10

71804 Hexavalent Chromium 8/4/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.5 100

NH 4 

# OH 8.5 9.5 10

71793 Total Cyanide 8/4/10 NaOH 7 10 15

71797 Total Cyanide 8/4/10 NaOH 7 10 10

71801 Total Cyanide 8/4/10 NaOH 6.7 10 15

71801 Total Cyanide-QC 8/4/10 NaOH 6.7 10 15

71803 Total Cyanide 8/4/10 NaOH 7 10 10

71794 Group II Volume 1 8/5/10 H 2 

# SO 4 

# 6 2 28

71794 Group II Volume 2 8/5/10 H 2 

# SO 4 

# 6 2 28

71797 Group II Volume 1 8/5/10 H 2 

# SO 4 

# 5 2 20

71797 Group II Volume 2 8/5/10 H 2 

# SO 4 

# 5 2 20

71801 Group II Volume 1 8/5/10 H 2 

# SO 4 

# 5 2 26

71801 Group II Volume 2 8/5/10 H 2 

# SO 4 

# 5 2 26

71801 Group II Volume 1-QC 8/5/10 H 2 

# SO 4 

# 5 2 26

71801 Group II Volume 2-QC 8/5/10 H 2 

# SO 4 

# 5 2 26

71803 Group II Volume 1-QC 8/5/10 H 2 

# SO 4 

# 5 2 20

71803 Group II Volume 2-QC 8/5/10 H 2 

# SO 4 

# 5 2 20

71794 Hexavalent Chromium 8/5/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.1 100

NH 4 

# OH 8.1 9.5 14

# 3-30 Table 3-7. Sample Preservation Summary, Allen

Sampling Point

Number Analysis Date Chemical

Added Initial pH Final pH Amount

Added

(drops)

71797 Hexavalent Chromium 8/5/10 (NH 4 

# ) 2 

# SO 4



# Buffer 5 8.7 100

NH 4 

# OH 8.7 9.5 8

71801 Hexavalent Chromium 8/5/10 (NH 4 

# ) 2 

# SO 4



# Buffer 5 8.7 100

NH 4 

# OH 8.7 9.5 10

71803 Hexavalent Chromium 8/5/10 (NH 4 

# ) 2 

# SO 4



# Buffer 5 8.7 100

NH 4 

# OH 8.7 9.5 8

71797 Total Cyanide 8/5/10 NaOH 7 10 10

71798 Total Cyanide 8/5/10 NaOH 7 10 10

71798 Total Cyanide-QC 8/5/10 NaOH 7 10 10

71802 Total Cyanide 8/5/10 NaOH 7 10 15

71798 Group II Volume 1 8/6/10 H 2 

# SO 4 

# 5 2 24

71798 Group II Volume 2 8/6/10 H 2 

# SO 4 

# 5 2 24

71798 Group II Volume 1-QC 8/6/10 H 2 

# SO 4 

# 5 2 24

71798 Group II Volume 2-QC 8/6/10 H 2 

# SO 4 

# 5 2 24

71802 Group II Volume 2 8/6/10 H 2 

# SO 4 

# 6 2 28

71802 Group II Volume 1 8/6/10 H 2 

# SO 4 

# 6 2 28

71798 Hexavalent Chromium 8/6/10 (NH 4 

# ) 2 

# SO 4



# Buffer 5 8.5 100

NH 4 

# OH 8.5 9.5 8

71802 Hexavalent Chromium 8/6/10 (NH 4 

# ) 2 

# SO 4



# Buffer 6 8.5 100

NH 4 

# OH 8.5 9.5 8

# 3-31 Table 3-8. Generating Unit Operating Charac teristics during Sample Collection, Allen

Unit ID Boiler Type Capacity

(MW) Electricity Production

(MW)

(or Percent Capacity %) SCR

(No, On, Off) Particulate Control System

(HS/CS ESP, or BH)

1 Tangential Fired 185 65.9% On CS ESP

2 Tangential Fired 185 64.3% On CS ESP

3 Tangential Fired 300 62.3% On CS ESP

4 Tangential Fired 300 75.3% On CS ESP

5 Tangential Fired 300 60.3% On CS ESP



# CS ESP – Cold-side elect rostatic precipitator.

# 3-32Table 3-9. FGD Scrubber System Operating Characteristics, AllenUnit IDWet FGDSystem (Yes/No)Type ofScrubberForcedOxidation(Yes/No)SorbentAdditives (DBA,formic acid, etc.)FGD Make-upWater SourceSO2RemovalPercentageType of SolidsSeparation1 Yes Spray Yes LimestoneNone Catawba River 96%SecondaryHydrocyclones3 Yes Spray Yes LimestoneNone Catawba River 97%SecondaryHydrocyclones

# 3-33 Table 3-10. Coal Usage during Sample Collection, Allen

Day of

Sampling Unit ID Coal Type Amount of Coal

Used During Day

of Sampling

(tpd) Source of Coal

(Coal Region

and/or State) Percent

Sulfur in

Coal

(%) Chlorine Content of

Coal

(ppm)

1 1 CAPP 1,335 WV/V 0.96 1,200 a



# 2 CAPP 1,378 WV/V 0.96 1,200 a



# 3 CAPP 736 WV/V 0.96 1,200 a



# 4 CAPP 1,212 WV/V 0.96 1,200 a



# 5 CAPP 1,097 WV/V 0.97 1,200 a



# 2 1 CAPP 662 KY 0.98 1,289

2 CAPP 500 KY 0.98 1,289

3 CAPP 1,799 KY 0.98 1,317

4 CAPP 2,386 KY 0.98 1,317

5 CAPP 1,221 KY 0.96 1,266

3 1 CAPP 1,645 KY 1.0 1,327

2 CAPP 1,750 KY 1.0 1,327

3 CAPP 3,081 KY 0.99 1,353

4 CAPP 2,805 KY 0.99 1,353

5 CAPP 3,221 KY 0.99 1,297

4 1 CAPP 1,228 KY 0.98 1,069

2 CAPP 1,137 KY 0.98 1,069

3 CAPP 2,260 KY 0.96 1,092

4 CAPP 2,445 KY 0.96 1,092

5 CAPP 2,102 KY 1.01 1,061

a 

# – Estimated value.

CAPP – Central Appalachian Coal.

[QUESTION FOR ALLEN: Please confirm that “WV/V” stands for West Virginia and Virginia.]

# 3-34 Table 3-11. Scrubber Slurry Information during Sample Collection, Allen

Day of

Sampling FGD

System ID Associated

Unit IDs Scrubber Slurry

Blowdown Flow Rate 

# Scrubber

Slurry

Percent

Solids Time of

Dewatering

During

Sampling Day Percent

Filtrate

Recycled

Back to

Scrubber Flow Rate

(gpm) Duration

1 1 1/2/5 URE a 

# 13 hrs 13% dewatered 5 times 100%

3 3/4 URE a 

# 13 hrs 12% dewatered 5 times 100%

2 1 1/2/5 URE a 

# 12 hrs 14% dewatered 5 times 100%

3 3/4 URE a 

# 9 hrs 14% dewatered 4 times 100%

3 1 1/2/5 URE a 

# 9 hrs 15% dewatered 4 times 100%

3 3/4 URE a 

# 8 hrs 15% dewatered 5 times 100%

4 1 1/2/5 URE a 

# 7 hrs 15% dewatered 3 times 100%

3 3/4 URE a 

# 6 hrs 15% dewatered 3 times 100%

a 

# – Unable to reasonably estimate.

# 3-35Table 3-12. FGD Wastewater Treatment System Operation Information, AllenDay ofSamplingScrubber PurgeChemical UsageFlow Rate(gpm)Duration(hr)Lime35% FerricChlorideOrganosulfide:NAPolymer:Nalco 7130730 % HCl1 375 17pH – 8.5 S.U.1,200 lbs40 ppm19.6 gpdNA6.5 ppm3.3 gpdpH – 6.8 S.U.390 gpd2 371 16pH – 8.5 S.U.1,200 lbs40 ppm19.2 gpdNA6.5 ppm3.1 gpdpH – 6.8 S.U.400 gpd3 371 14pH – 8.5 S.U.1,200 lbs40 ppm19.3 gpdNA6.5 ppm3.1 gpdpH – 6.8 S.U.400 gpd4 366 10pH – 8.5 S.U.1,200 lbs40 ppm14.7 gpdNA6.5 ppm2.3 gpdpH – 6.8 S.U.390 gpd

# 4-1 4. L ABORATORY 

# A NALYTICAL 

# R ESULTS AND 

# D ISCUSSION



# This section summarizes the laboratory results of analyses of samples for the influent to

the FGD wastewater treatment system and the in fluent to and effluent from the bioreactor

system.

Appendix A provides all analytical results for all samples collected at Allen, including all

qualified results and all results that were meas ured above the method detection limit, but below

the quantitation limit (i.e., J-values).

Table 4-1 presents the analytical results for all classical and metal analytes for the four

days of sampling at the influent to the FGD wast ewater treatment system (SP-1), as well as the

calculated 4-day average result for each analyt e for the sampling point. Tables 4-2 and 4-3

present the analytical results for all classical and metal analytes fo r the four days of sampling at

the influent to the bioreactor system (SP-2) and the effluent from the bi oreactor system (SP-3),

respectively.

Tables 4-1 through 4-3 include the analytical results for all analytes for which EPA

conducted analyses. Results are rounded to thre e significant digits. If an analyte was not

detected in a sample above the method detection limit, the result is reported as “ND” followed by

the sample-specific quantitation limit in parenthe ses. If an analyte was measured above the

method detection limit, but below the quantitation limit, the result is reported as “NQ” followed

by the sample-specific quantitation limit in parentheses.

For Table 4-2 (influent to the bioreactor system), a duplicate sample was collected on

Day 3 of the sampling episode. The Day 3 result s presented in Table 4-2 represent the average

of the original and the duplicate sample results. Table 5-1 presents the analytical results for the

original and duplicate results and the calculated re lative percent difference (RPD). For Table 4-3

(effluent from the bioreactor system), a duplicat e sample was collected on Day 2 of the sampling

episode. The Day 2 results presented in Table 4- 3 represent the average of the original and the

duplicate sample results. Table 5-2 presents the analytical results for the original and duplicate

results and the calculated RPD.

When calculating the average concentrations for the duplicate samples or for the 4-day

averages, ERG used the following methodology:

 

# If all results were above the quantitati on limit, then all results were averaged

together;

 

# If at least one result was above the quan titation limit, then 1⁄2 the quantitation limit

was used in the average calculation for all results that were less than the

quantitation limit and the result is repo rted with a less than sign (“<”);

 

# If all results were below the quantitation limit, but at least one result was above

the method detection limit (i.e., J-value), then the sample-specific quantitation

limits for all results were averaged and the result is reported as “NQ” followed by

the average of the sample-specific quantitation limits; and

 

# If all results were below the method de tection limit, then the sample-specific

quantitation limits for all results were aver aged and the result is reported as “ND”

followed by the average of the sample-specific quantitation limits.

# 4-2 Table 4-4 presents the 4-day average results fo r all classical analytes for the influent to

the FGD wastewater treatment system (SP-1), the influent to the bioreact or system (SP-2), and

the effluent from the bioreactor system (SP-3). Table 4-5 presen ts the 4-day average results for

all metal analytes, including total and dissolved an alyses, for the influent to the FGD wastewater

treatment system (SP-1), the influent to the bior eactor system (SP-2), and the effluent from the

bioreactor system (SP-3). The re sults presented in this report are not paired results, meaning that

the sample collection across the treatment system was not timed to reflect the amount of time it

takes the wastewater to pass through the system.

Appendix Tables A-1 through A-14 present all the analytical results from the influent to

the FGD wastewater treatment system (SP-1), the influent to the bioreact or system (SP-2), the

effluent from the bioreactor syst em (SP-3), the duplicate of the in fluent to the bioreactor system

(SP-4), and the duplicate of the effluent from th e bioreactor system ( SP-5) sample analyses,

including J-values.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 4-3Table 4-1. Influent to FGD Wastewater Treatment System, AllenAnalyteUnitsMethodInfluent to FGD Wastewater Treatment System (SP-1)Day 1Day 2Day 3Day 44-DayAverageClassicalsAmmoniamg/L350.11.407.3014.09.007.93Nitrate Nitrite as Nmg/L353.214.017.020.022.018.3Nitrogen, Total Kjeldahlmg/L351.216.021.026.045.027.0Biochemical Oxygen Demandmg/L5210B40.0B9.70B9.406.6016.4Chemical Oxygen Demandmg/L410.4110*NQ (200)*130140< 120Chloridemg/L300.02,8003,4004,2005,2003,900Sulfatemg/L300.01,4001,3001,3001,3001,330Cyanide, Totalmg/L4500 CN E0.2500.3400.1900.240B0.255Total Dissolved Solidsmg/L2540C7,0008,90011,00011,0009,480Total Suspended Solidsmg/L2540D1,8003,9002,0002,2002,480Phosphorus, Totalmg/L365.10.350NQ (0.0500)NQ (0.0500)NQ (0.0500)< 0.106Total MetalsAluminumug/L200.770,00076,00072,00071,00072,300Antimonyug/L200.89.3011.011.012.010.8Arsenicug/L200.8120140140140135Bariumug/L200.7750880930990888Berylliumug/L200.710.012.012.012.011.5Boronug/L200.754,000B62,000B80,000B100,000B74,000Cadmiumug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)4.00< 2.50Calciumug/L200.71,900,0002,300,0002,400,0002,700,0002,330,000Chromiumug/L200.8120^140^130^140^133Cobaltug/L200.752.059.064.067.060.5Copperug/L200.8140160160180160Ironug/L200.764,00069,00068,00070,00067,800Leadug/L200.885.099.0100120101< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 4-4Table 4-1. Influent to FGD Wastewater Treatment System, AllenAnalyteUnitsMethodInfluent to FGD Wastewater Treatment System (SP-1)Day 1Day 2Day 3Day 44-DayAverageMagnesiumug/L200.7440,000470,000520,000590,000505,000Manganeseug/L200.73,3003,7004,1004,6003,930Mercuryng/L1631E50,30049,60044,60052,10049,200Molybdenumug/L200.7NQ (40.0)NQ (40.0)NQ (40.0)41.0< 25.3Nickelug/L200.8160190190210188Seleniumug/L200.81,5001,7001,8001,8001,700Silverug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Sodiumug/L200.729,00032,00034,00038,00033,300Thalliumug/L200.82.503.203.303.103.03Tinug/L200.7NQ (200)NQ (200)NQ (200)NQ (200)NQ (200)Titaniumug/L200.71,4001,5001,6001,6001,530Vanadiumug/L200.8130150160180155Zincug/L200.8220280280330278Dissolved MetalsAluminumug/L200.7NQ (200)NQ (200)ND (200)ND (200)NQ (200)Antimonyug/L200.82.002.803.504.003.08Arsenicug/L200.88.7010.010.07.108.95Bariumug/L200.7250310410500368Berylliumug/L200.7ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Boronug/L200.750,000B59,000B80,000B100,000B72,300Cadmiumug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Calciumug/L200.71,500,0001,700,0002,000,0002,500,0001,930,000Chromiumug/L200.8ND (8.00)ND (8.00)ND (8.00)ND (8.00)ND (8.00)Chromium (VI)ug/L218.6ND (10.0)ND (10.0)ND (10.0)ND (10.0)ND (10.0)Cobaltug/L200.7NQ (20.0)20.022.025.0< 19.3Copperug/L200.812.012.011.08.9011.0< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 4-5Table 4-1. Influent to FGD Wastewater Treatment System, AllenAnalyteUnitsMethodInfluent to FGD Wastewater Treatment System (SP-1)Day 1Day 2Day 3Day 44-DayAverageIronug/L200.7ND (200)ND (200)ND (200)ND (200)ND (200)Leadug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Magnesiumug/L200.7400,000440,000500,000550,000473,000Manganeseug/L200.72,8003,1003,6004,0003,380Mercuryng/L1631E15.221.016.475.031.9Molybdenumug/L200.7NQ (40.0)NQ (40.0)NQ (40.0)NQ (40.0)NQ (40.0)Nickelug/L200.871.077.080.093.080.3Seleniumug/L200.8230290340400315Silverug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Sodiumug/L200.728,00031,00036,00040,00033,800Thalliumug/L200.8NQ (2.00)NQ (2.00)NQ (2.00)NQ (2.00)NQ (2.00)Tinug/L200.7ND (200)ND (200)ND (200)ND (200)ND (200)Titaniumug/L200.7NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)Vanadiumug/L200.8ND (10.0)NQ (10.0)NQ (10.0)NQ (10.0)NQ (10.0)Zincug/L200.830.043.032.043.037.0Note: The Day 3 results represent the average of the original sample and the field duplicate (SP-4). See Section 5.2.4 for theanalytical results for the individual samples.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.E – MS and/or MSD exceeded the calibration range.F – MS and/or MSD not within laboratory control limits.



# 4-6Table 4-2. Influent to the Bioreactor System, AllenAnalyteUnitsMethodInfluent to the Bioreactor System (SP-2)Day 1Day 2Day 3Day 44-DayAverageClassicalsAmmoniamg/L350.15.806.808.8511.08.11Nitrate Nitrite as Nmg/L353.214.013.013.013.0E,F13.3Nitrogen, Total Kjeldahlmg/L351.27.708.6010.53.20F7.50Biochemical Oxygen Demandmg/L5210B7.40B5.203.653.905.04Chemical Oxygen Demandmg/L410.4NQ (200)*79.0< 72.084.0F< 83.8Chloridemg/L300.03,1003,2003,3003,6003,300Sulfatemg/L300.01,4001,4001,4001,4001,400Cyanide, Totalmg/L4500 CN ENQ (0.100)NQ (0.100)0.1300.110B< 0.0850Total Dissolved Solidsmg/L2540C7,5007,8007,1507,8007,560Total Suspended Solidsmg/L2540DNQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Phosphorus, Totalmg/L365.10.0610NQ (0.0500)NQ (0.0500)NQ (0.0500)< 0.0340Total MetalsAluminumug/L200.7ND (200)ND (200)ND (200)NQ (200)BNQ (200)Antimonyug/L200.82.202.202.302.902.40Arsenicug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Bariumug/L200.7220200220250223Berylliumug/L200.7ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Boronug/L200.755,000B54,000B59,00064,000B,458,000Cadmiumug/L200.8ND (4.00)ND (4.00)ND (4.00)ND (4.00)ND (4.00)Calciumug/L200.71,600,0001,600,0001,700,0001,800,00041,680,000Chromiumug/L200.8ND (8.00)^ND (8.00)^ND (8.00)ND (8.00)^ND (8.00)Note: The Day 3 results represent the average of the original sample and the field duplicate (SP-4). See Section 5.2.4 for theanalytical results for the individual samples.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.E – MS and/or MSD exceeded the calibration range.F – MS and/or MSD not within laboratory control limits.



# 4-7Table 4-2. Influent to the Bioreactor System, AllenAnalyteUnitsMethodInfluent to the Bioreactor System (SP-2)Day 1Day 2Day 3Day 44-DayAverageCobaltug/L200.7ND (20.0)ND (20.0)ND (20.0)ND (20.0)ND (20.0)Copperug/L200.810.09.3011.012.010.6Ironug/L200.7NQ (200)ND (200)NQ (200)NQ (200)BNQ (200)Leadug/L200.8ND (2.00)ND (2.00)ND (2.00)NQ (2.00)NQ (2.00)Magnesiumug/L200.7420,000400,000420,000420,0004415,000Manganeseug/L200.7400340440520B425Mercuryng/L1631E7111,3209911,1201,040Molybdenumug/L200.7NQ (40.0)NQ (40.0)NQ (40.0)NQ (40.0)NQ (40.0)Nickelug/L200.88.207.908.107.908.03Seleniumug/L200.811094.088.587.094.9Silverug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Sodiumug/L200.731,00029,00030,50031,00030,400Thalliumug/L200.8NQ (2.00)NQ (2.00)NQ (2.00)NQ (2.00)NQ (2.00)Tinug/L200.7ND (200)ND (200)ND (200)ND (200)ND (200)Titaniumug/L200.7NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)Vanadiumug/L200.8ND (10.0)ND (10.0)ND (10.0)ND (10.0)ND (10.0)Zincug/L200.8NQ (10.0)NQ (10.0)NQ (10.0)NQ (10.0)NQ (10.0)Dissolved MetalsAluminumug/L200.7ND (200)ND (200)ND (200)ND (200)ND (200)Antimonyug/L200.82.402.302.252.602.39Arsenicug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Bariumug/L200.7220210220250225Note: The Day 3 results represent the average of the original sample and the field duplicate (SP-4). See Section 5.2.4 for theanalytical results for the individual samples.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.E – MS and/or MSD exceeded the calibration range.F – MS and/or MSD not within laboratory control limits.



# 4-8Table 4-2. Influent to the Bioreactor System, AllenAnalyteUnitsMethodInfluent to the Bioreactor System (SP-2)Day 1Day 2Day 3Day 44-DayAverageBerylliumug/L200.7ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Boronug/L200.757,000B56,000B58,50063,000B,458,600Cadmiumug/L200.8ND (4.00)ND (4.00)ND (4.00)ND (4.00)ND (4.00)Calciumug/L200.71,700,0001,700,0001,700,0001,900,00041,750,000Chromiumug/L200.8ND (8.00)ND (8.00)ND (8.00)ND (8.00)ND (8.00)Chromium (VI)ug/L218.6ND (10.0)ND (10.0)ND (10.0)ND (10.0)ND (10.0)Cobaltug/L200.7ND (20.0)NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)Copperug/L200.810.08.9011.012.010.5Ironug/L200.7ND (200)ND (200)ND (200)ND (200)ND (200)Leadug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Magnesiumug/L200.7410,000370,000405,000400,0004396,000Manganeseug/L200.7370320410470393Mercuryng/L1631E219483304361342Molybdenumug/L200.7NQ (40.0)NQ (40.0)NQ (40.0)NQ (40.0)NQ (40.0)Nickelug/L200.89.207.408.308.208.28Seleniumug/L200.898.093.086.587.091.1Silverug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Sodiumug/L200.731,00031,00032,00031,00031,300Thalliumug/L200.8NQ (2.00)NQ (2.00)NQ (2.00)NQ (2.00)NQ (2.00)Tinug/L200.7ND (200)ND (200)ND (200)ND (200)ND (200)Titaniumug/L200.7NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)Vanadiumug/L200.8ND (10.0)ND (10.0)NQ (10.0)ND (10.0)ND (10.0)Note: The Day 3 results represent the average of the original sample and the field duplicate (SP-4). See Section 5.2.4 for theanalytical results for the individual samples.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.E – MS and/or MSD exceeded the calibration range.F – MS and/or MSD not within laboratory control limits.



# 4-9Table 4-2. Influent to the Bioreactor System, AllenAnalyteUnitsMethodInfluent to the Bioreactor System (SP-2)Day 1Day 2Day 3Day 44-DayAverageZincug/L200.812.0NQ (10.0)NQ (10.0)NQ (10.0)< 6.75Note: The Day 2 results represent the average of the original sample and the field duplicate (SP-5). See Section 5.2.4 for theanalytical results for the individual samples.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.



# 4-10Table 4-3. Effluent from the Bioreactor System, AllenAnalyteUnitsMethodEffluent from the Bioreactor System (SP-3)Day 1Day 2Day 3Day 44-DayAverageClassicalsAmmoniamg/L350.113.0F11.011.012.011.8Nitrate Nitrite as Nmg/L353.2NQ (0.100)0.130ND (0.100)NQ (0.100)< 0.0700Nitrogen, Total Kjeldahlmg/L351.214.0412.512.013.012.9Biochemical Oxygen Demandmg/L5210BNQ (2.50)BNQ (2.50)ND (2.00)NQ (2.50)NQ (2.38)Chemical Oxygen Demandmg/L410.4NQ (200)*67.0NQ (100)F36.0< 63.3Chloridemg/L300.03,4003,2503,2003,2003,260Sulfatemg/L300.01,5001,5001,4001,4001,450Cyanide, Totalmg/L4500 CN E0.110< 0.08000.120B0.100B< 0.103Total Dissolved Solidsmg/L2540C8,0008,4007,1007,5007,750Total Suspended Solidsmg/L2540DNQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Phosphorus, Totalmg/L365.10.1100.1600.190F0.1500.153Total MetalsAluminumug/L200.7NQ (200)NQ (200)NQ (200)NQ (200)BNQ (200)Antimonyug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Arsenicug/L200.8ND (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Bariumug/L200.7210215210220214Berylliumug/L200.7ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Boronug/L200.765,000B,466,00063,000B,461,000B63,800Cadmiumug/L200.8ND (4.00)ND (4.00)ND (4.00)ND (4.00)ND (4.00)Calciumug/L200.71,700,00041,650,0001,600,00041,600,0001,640,000Chromiumug/L200.8ND (8.00)^ND (8.00)ND (8.00)^ND (8.00)^ND (8.00)Cobaltug/L200.7ND (20.0)ND (20.0)ND (20.0)ND (20.0)ND (20.0)Note: The Day 2 results represent the average of the original sample and the field duplicate (SP-5). See Section 5.2.4 for theanalytical results for the individual samples.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.



# 4-11Table 4-3. Effluent from the Bioreactor System, AllenAnalyteUnitsMethodEffluent from the Bioreactor System (SP-3)Day 1Day 2Day 3Day 44-DayAverageCopperug/L200.8ND (4.00)ND (4.00)ND (4.00)ND (4.00)ND (4.00)Ironug/L200.7NQ (200)NQ (200)210NQ (200)B< 128Leadug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Magnesiumug/L200.7450,0004435,000410,0004420,000429,000Manganeseug/L200.7470435400440B436Mercuryng/L1631E12.630.027.717.021.8Molybdenumug/L200.7NQ (40.0)NQ (40.0)NQ (40.0)ND (40.0)NQ (40.0)Nickelug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Seleniumug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Silverug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Sodiumug/L200.733,00032,50031,00031,00031,900Thalliumug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Tinug/L200.7ND (200)ND (200)ND (200)ND (200)ND (200)Titaniumug/L200.7NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)Vanadiumug/L200.8ND (10.0)ND (10.0)ND (10.0)ND (10.0)ND (10.0)Zincug/L200.8NQ (10.0)NQ (10.0)15.0NQ (10.0)< 7.50Dissolved MetalsAluminumug/L200.7NQ (200)NQ (200)NQ (200)NQ (100)NQ (175)Antimonyug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Arsenicug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Bariumug/L200.7220220210110190Berylliumug/L200.7ND (2.00)ND (2.00)ND (2.00)ND (1.00)ND (1.75)Boronug/L200.771,000B,466,50063,000B,457,000B64,400Note: The Day 2 results represent the average of the original sample and the field duplicate (SP-5). See Section 5.2.4 for theanalytical results for the individual samples.< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.



# 4-12Table 4-3. Effluent from the Bioreactor System, AllenAnalyteUnitsMethodEffluent from the Bioreactor System (SP-3)Day 1Day 2Day 3Day 44-DayAverageCadmiumug/L200.8ND (4.00)ND (4.00)ND (4.00)ND (4.00)ND (4.00)Calciumug/L200.71,900,00041,700,0001,600,00041,700,0001,730,000Chromiumug/L200.8ND (8.00)ND (8.00)ND (8.00)ND (8.00)ND (8.00)Chromium (VI)ug/L218.6ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Cobaltug/L200.7ND (20.0)ND (20.0)ND (20.0)ND (10.0)ND (17.5)Copperug/L200.8ND (4.00)ND (4.00)ND (4.00)ND (4.00)ND (4.00)Ironug/L200.7NQ (200)BNQ (200)NQ (200)BNQ (100)BNQ (175)Leadug/L200.8ND (2.00)NQ (2.00)ND (2.00)ND (2.00)NQ (2.00)Magnesiumug/L200.7440,0004425,000400,0004190,000364,000Manganeseug/L200.7440405380190354Mercuryng/L1631E10.433.428.315.922.0Molybdenumug/L200.7NQ (40.0)ND (40.0)ND (40.0)NQ (20.0)NQ (35.0)Nickelug/L200.8NQ (4.00)NQ (4.00)ND (4.00)NQ (4.00)NQ (4.00)Seleniumug/L200.8NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Silverug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Sodiumug/L200.734,00033,50032,00016,00028,900Thalliumug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Tinug/L200.7ND (200)ND (200)ND (200)ND (100)ND (175)Titaniumug/L200.7NQ (20.0)NQ (20.0)NQ (20.0)NQ (10.0)NQ (17.5)Vanadiumug/L200.8ND (10.0)ND (10.0)ND (10.0)ND (10.0)ND (10.0)Zincug/L200.8NQ (10.0)NQ (10.0)NQ (10.0)NQ (10.0)NQ (10.0)< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.



# 4-13Table 4-4. Classical Pollutants 4-Day Average DataSummary for FGD Wastewater Treatment System, AllenAnalyteUnitsMethodInfluent to FGDWastewater TreatmentSystem (SP-1)4-Day AverageInfluent toBioreactorSystem (SP-2)4-Day AverageEffluent fromBioreactorSystem (SP-3)4-Day AverageClassicalsAmmoniamg/L350.17.938.1111.8Nitrate Nitrite as Nmg/L353.218.313.3< 0.0700Nitrogen, Total Kjeldahlmg/L351.227.07.5012.9Biochemical Oxygen Demandmg/L5210B16.45.04NQ (2.38)Chemical Oxygen Demandmg/L410.4< 120< 83.8< 63.3Chloridemg/L300.03,9003,3003,260Sulfatemg/L300.01,3301,4001,450Cyanide, Totalmg/L4500 CN E0.255< 0.0850< 0.103Total Dissolved Solidsmg/L2540C9,4807,5607,750Total Suspended Solidsmg/L2540D2,480NQ (4.00)NQ (4.00)Phosphorus, Totalmg/L365.1< 0.106< 0.03400.153< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.



# 4-14Table 4-5. Metal Pollutants 4-Day Average Data Summary for FGD Wastewater Treatment System, AllenAnalyteUnitsMethodInfluent to FGDWastewater TreatmentSystem (SP-1)4-Day AverageInfluent toBioreactorSystem (SP-2)4-Day AverageEffluent fromBioreactorSystem (SP-3)4-Day AverageTotalDissolvedTotalDissolvedTotalDissolvedMetalsAluminumug/L200.772,300NQ (200)NQ (200)ND (200)NQ (200)NQ (175)Antimonyug/L200.810.83.082.402.39ND (2.00)ND (2.00)Arsenicug/L200.81358.95NQ (4.00)NQ (4.00)NQ (4.00)NQ (4.00)Bariumug/L200.7888368223225214190Berylliumug/L200.711.5ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (1.75)Boronug/L200.774,00072,30058,00058,60063,80064,400Cadmiumug/L200.8< 2.50NQ (4.00)ND (4.00)ND (4.00)ND (4.00)ND (4.00)Calciumug/L200.72,330,0001,930,0001,680,0001,750,0001,640,0001,730,000Chromiumug/L200.8133ND (8.00)ND (8.00)ND (8.00)ND (8.00)ND (8.00)Chromium (VI)ug/L218.6NAND (10.0)NAND (10.0)NAND (2.00)Cobaltug/L200.760.5< 19.3ND (20.0)NQ (20.0)ND (20.0)ND (17.5)Copperug/L200.816011.010.610.5ND (4.00)ND (4.00)Ironug/L200.767,800ND (200)NQ (200)ND (200)< 128NQ (175)Leadug/L200.8101ND (2.00)NQ (2.00)ND (2.00)ND (2.00)NQ (2.00)Magnesiumug/L200.7505,000473,000415,000396,000429,000364,000Manganeseug/L200.73,9303,380425393436354Mercuryng/L1631E49,20031.91,04034221.822.0Molybdenumug/L200.7< 25.3NQ (40.0)NQ (40.0)NQ (40.0)NQ (40.0)NQ (35.0)Nickelug/L200.818880.38.038.28NQ (4.00)NQ (4.00)Seleniumug/L200.81,70031594.991.1NQ (4.00)NQ (4.00)Silverug/L200.8ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)ND (2.00)Sodiumug/L200.733,30033,80030,40031,30031,90028,900Thalliumug/L200.83.03NQ (2.00)NQ (2.00)NQ (2.00)ND (2.00)ND (2.00)Tinug/L200.7NQ (200)ND (200)ND (200)ND (200)ND (200)ND (175)Titaniumug/L200.71,530NQ (20.0)NQ (20.0)NQ (20.0)NQ (20.0)NQ (17.5)< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.



# 4-15Table 4-5. Metal Pollutants 4-Day Average Data Summary for FGD Wastewater Treatment System, AllenAnalyteUnitsMethodInfluent to FGDWastewater TreatmentSystem (SP-1)4-Day AverageInfluent toBioreactorSystem (SP-2)4-Day AverageEffluent fromBioreactorSystem (SP-3)4-Day AverageTotalDissolvedTotalDissolvedTotalDissolvedVanadiumug/L200.8155NQ (10.0)ND (10.0)ND (10.0)ND (10.0)ND (10.0)Zincug/L200.827837.0NQ (10.0)< 6.75< 7.50NQ (10.0)

# 5-1 5. D ATA 

# Q UALITY



# Quality assurance/quality control (QA/QC) pr ocedures applicable to the Allen sampling

episode are outlined in the Quality Assurance Project Plan for the Steam Electric Rulemaking

(QAPP) [2]. This section describes the QC procedur es used to assess the precision and accuracy

of the analytical data presented in Section 4 and Appendix A. QC practices used for this

sampling episode include the analysis of matr ix spikes, blanks, duplicate samples, and QC

standard checks.

5.1 Analytical Quality Control

ERG contracted directly with analytical labor atories for the analysis of samples collected

during this episode. ERG verified that the pe rformance of the laboratories selected was

acceptable by conducting QC checks of the analytical data. Data review chemists at ERG

prepared written data review narr atives describing any qualifications of the analytical data from

this episode. The data review narrat ives are included in Appendix B.

5.2 Field Quality Control

This subsection discusses the bottle blanks, equipment blanks, field blanks, and field

duplicate sample results. Section 3.8 of the Samp ling Plan discusses fiel d QC specifications.

5.2.1 Bottle Blanks

ERG collects bottle blanks to evaluate possibl e contamination from the sample collection

bottles. Bottle blanks were collected and analyzed for metals (EPA Method 200.8, 200.7, and

1631E) by the supplier of the precleane d bottles, Frontier Global Sciences.

Frontier Global Sciences collected the bottl e blanks for the metals sample bottles by

filling the precleaned bottle with reagent water, pres erving the sample with nitric acid to a pH <2

S.U. Frontier Global Sciences then an alyzed the samples using EPA Method 1638.

Frontier Global Sciences collected the bottl e blanks for the mercury sample bottles by

filling the precleaned bottle with reagent wa ter, preserving the sample with bromine

monochloride. Frontier Global Sciences then analyzed the samples using EPA Method 1631E.

Albion Environmental collected the bottle bl anks for the 10- and 20-liter composite

containers by filling the container with approx imately two liters of ASTM Type 1 water and

allowing it to soak for approximately 24 hours. During this period, th e laboratory shook the

container periodically and changed its orientation to expose all surfaces of the container to the

ASTM Type 1 water. Albion Environmental th en shook the 10- and 20-liter containers and

poured a representative sample into a precleaned LDPE sample bottle. The blank samples were

then preserved and analyzed according to the analytical methods (i.e., EPA Method 1638).

No metals were detected in the bottle bl anks, except for aluminum in the 10-liter

composite containers. Aluminum was detected in the 10-L container at a concentration of 4.71

ug/L, which is well below the quantitation limit us ed for the sample analyses (i.e., 200 ug/L);

therefore, this contamination di d not affect the resu lts of the sampling episode. Appendix Table

A-15 presents all of the analytical results from the bottle blank an alyses for the bottles used to

collect the metals and mercury samples.

# 5-2 5.2.2 Equipment Blanks

EPA collects equipment blanks to evaluate possible contamination from the equipment

used for sample collection. The techniques used to collect field blanks (a s described in Section

5.2.3) include the sampling equipment and ther efore would identify whether the sampling

equipment was introducing contamination. In a ddition, Frontier Global Sciences collected and

analyzed equipment blanks for metals (EPA Method 1638 and 1631E) for each type of

precleaned equipment supp lied for the episode.

Frontier Global Sciences collected the equipment blanks for the capsule filters and

fabricated tubing sets by pumping reagent wa ter through the cleaned and fully assembled

equipment sets (with the filter attached) into a cleaned bottle (an HDPE bottle for metals and a

glass bottle for mercury). The blank samples were then preserved and analyzed according to the

analytical methods (EPA Method 1638 and 1631E), as described in Section 5.2.1 for the bottle

blank samples.

No other metals were detected in the e quipment blanks, indicating that there was no

contamination from the sampling equipment. Appe ndix Table A-15 presents all of the analytical

results from the bottle blank and equipment blank analyses for all of the equipment used for the

collection of metals and mercury samples.

5.2.3 Field Blanks

The sampling team collected field blanks (SP-6, SP-7, and SP-8) to evaluate possible

contamination from sampling equipment, samp ling equipment decontamination procedures,

sample collection procedures, or atmospheric contamination. EPA analyzed the field blank

samples for the total and dissolved metals and total and dissolved mercury samples 1



# .

The total mercury, total metals by 200.8, and total metals by 200.7 field blank samples

for the influent to the FGD wastewater treatm ent system (SP-6) were collected by pouring

ASTM Type 1 water through a precle aned funnel attached to the si licone tubing used for sample

collection. To prevent potential atmospheric deposition, the sampling team shrouded the blank

water and funnel with a plastic bag while pouring the blank water into the funnel and placed the

sample bottles in a “cleanbox.” Prior to filli ng the sample bottles, the tubing was purged with

approximately 1 liter of blank water. The ASTM Type 1 water flowed through the funnel, tubing,

and “Y” splitter, and was poured into the sample bottles. EPA collected the field blank samples

as “true” split samples with Allen’s representati ve, using the same procedure discussed in the

sample collection description (Section 3.1).

The dissolved metals by 200.8, dissolved metals by 200.7, and dissolved hexavalent

chromium field blank samples for the influent to the FGD wastew ater treatment system (SP-6)

were collected by pouring ASTM Type 1 water th rough the funnel and tubi ng and into a 10-liter

composite container. After approximately 90 mi nutes, the sampling team began pumping off the

ASTM Type 1 water from the 10-liter composite c ontainer. Prior to filling the sample bottles, the

1 

# EPA did not collect a dissolved mercury field blank samp le because the sample filtration was performed at the

laboratory to minimize potential mercury contamination. The laboratory performed a filtration blank prior to

filtering the sample, which is assessed as the field bl ank for the dissolved mercury sample. Mercury was not

detected above the method detection limit (0.24 ng/L) in the filtration blanks performed fo r the first, second, third,

fourth, or fifth day of samples.

# 5-3 tubing was purged with approximately 0.75 liters of blank water. The sample bottles were placed

in a “cleanbox” and the ASTM Type 1 water wa s pumped through the tubing, capsule filter, and

“Y” splitter, and into the sample bottles. EPA co llected the field blank samples as “true” split

samples with Allen’s representative, using the sa me procedure discussed in the sample collection

description (Section 3.1).

The total mercury field blank sample for the in fluent to the bioreactor system (SP-7) and

for the effluent from the biorea ctor system (SP-8) were collect ed by shrouding one of the three

taps on the sample tap splitter with a clear pl astic bag. Before purging the sample tap, ASTM

Type 1 water was poured directly into the sample bottles under th e clear plastic bag.

The total and dissolved metals by 200.7 and metals by 200.8 field blank samples for the

influent to the bioreactor system (SP-7) and the effluent from th e bioreactor system (SP-8) were

collected by filling a 20-liter composite container with ASTM Type 1 water. The same T-splitter

tubing used to fill the metals composite container at each point was used with a pump to fill the

20-liter field blank composite cont ainer. Prior to attaching one e nd of the tubing to the sample

tap, it was placed directly into the container hol ding ASTM Type 1 water. The other end of the

tubing was attached to the vented cap on top of the 20-liter field blank composite container.

After enough volume was pumped into the 20-liter field blank composite container, the vented

cap was replaced with a closed cap and the contai ner was placed on ice in a covered plastic trash

can. The vented cap used for the field blank coll ection was the same cap that was later used for

the metals composite container.

After 24 hours, the ASTM Type 1 water in th e 20-liter field bla nk composite container

was pumped off into the individual sample bottles. The sample bottles were placed in a

“cleanbox” and the ASTM Type 1 water was pump ed through the tubing and “Y” splitter, and

into the sample bottles. EPA collected the fiel d blank samples as “true” split samples with

Allen’s representative, using the same procedure discussed in the sample collection description

(Section 3.1). Prior to filling the sample bottles, the tubing wa s purged with approximately one

liter of ASTM Type 1 water. First the sample rs collected the total metals by 200.8 and total

metals by 200.7 field blank samples. Then the samp ling team attached a f ilter to the tubing set

and purged the tubing with approximately one liter of ASTM Type 1 water. The samplers then

collected the dissolved metals by 200.8, dissolv ed metals by 200.7, and dissolved hexavalent

chromium field blank samples.

No metals were detected in the field blank samples above the quantitation limit,

indicating that there was no cont amination from the sample collection procedures. Appendix

Tables A-16 through A-24 present al l the analytical results for the field blanks from each of the

sampling points, including all qua lified results and all results th at were measured above the

method detection limit, but below the quantitation limit (i.e., J-values).

5.2.4 Field Duplicates

The sampling team collected field duplicate samples to assess the sampling measurement

precision for the episode. Field dupl icate samples are separate samp les collected from the same

location as the original sample. The duplicate is filled immediately after the original sample and

is stored and analyzed independently. The RPD be tween the two sample results is examined to

determine the precision of the data. EPA used the following equation to calculate the RPD for

the duplicate sample results.

# 5-4 100

2 D S D S (%) RPD 

       

 

# (5-1)

where:

S = concentration of the analyte from original sample analysis;

D = concentration of the analyte from the duplicate sample analysis; and

RPD = relative percent difference between the original and duplicate sample

result.

During this sampling episode, EPA collected duplicate samples for the influent to the

bioreactor system (SP-4) and th e effluent from the bioreactor system (SP-5) during day 3 and

day 2 of the sampling episode, respectively. Th e duplicate samples were analyzed for all

analytes. Tables 5-1 and 5-2 present the anal ytical results and the RPD for SP-4 and SP-5,

respectively. Results are rounded to three sign ificant digits. Appendix Tables A-7 and A-13

present all the analytical results for the influent to the bioreactor syst em (SP-2) Day 3 and the

duplicate of the influent to the bioreactor system (SP-4) sample analyses , including all qualified

results and all results that were measured a bove the method detection limit, but below the

quantitation limit (i.e., J-values). Appendix Tabl es A-10 and A-14 present all the analytical

results for the effluent from the bioreactor system (SP-3) Day 2 and the du plicate of the effluent

from the bioreactor system (SP-5) sample analys es, including all qualified results and all results

that were measured above the method detectio n limit, but below the quantitation limit (i.e., Jvalues).< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).NC – Not calculated.ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 5-5 5-5Table 5-1. Field Duplicate Results – Influent to Bioreactor System, AllenAnalyteUnitsMethodOriginal Influentto BioreactorSystem, Day 3Concentration (SP-2)Duplicate Influentto BioreactorSystemConcentration (SP-4)AverageResultRelativePercentDifferenceClassicalsAmmonia350.1mg/L8.908.808.851.13Nitrate Nitrite as N353.2mg/L13.013.013.00.00Nitrogen, Total Kjeldahl351.2mg/L10.011.010.59.52Biochemical Oxygen Demand5210Bmg/L3.603.703.652.74Chemical Oxygen Demand410.4mg/LNQ (100)94.0< 72.0NCChloride300.0mg/L3,2003,4003,3006.06Sulfate300.0mg/L1,4001,4001,4000.00Cyanide, Total4500 CN Emg/L0.120B0.140B0.13015.4Total Dissolved Solids2540Cmg/L7,2007,1007,1501.40Total Suspended Solids2540Dmg/LNQ (4.00)NQ (4.00)NQ (4.00)NCPhosphorus, Total365.1mg/LNQ (0.0500)NQ (0.0500)NQ (0.0500)NCTotal MetalsAluminum200.7ug/LND (200)ND (200)ND (200)NCAntimony200.8ug/L2.302.302.300.00Arsenic200.8ug/LNQ (4.00)NQ (4.00)NQ (4.00)NCBarium200.7ug/L2202202200.00Beryllium200.7ug/LND (2.00)ND (2.00)ND (2.00)NCBoron200.7ug/L59,000B59,000B59,0000.00Cadmium200.8ug/LND (4.00)ND (4.00)ND (4.00)NCCalcium200.7ug/L1,700,0001,700,0001,700,0000.00Chromium200.8ug/LND (8.00)^ND (8.00)^ND (8.00)NCCobalt200.7ug/LND (20.0)ND (20.0)ND (20.0)NCCopper200.8ug/L11.011.011.00.00< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).NC – Not calculated.ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 5-6 5-6Table 5-1. Field Duplicate Results – Influent to Bioreactor System, AllenAnalyteUnitsMethodOriginal Influentto BioreactorSystem, Day 3Concentration (SP-2)Duplicate Influentto BioreactorSystemConcentration (SP-4)AverageResultRelativePercentDifferenceIron200.7ug/LND (200)NQ (200)NQ (200)NCLead200.8ug/LND (2.00)ND (2.00)ND (2.00)NCMagnesium200.7ug/L430,000410,000420,0004.76Manganese200.7ug/L4404404400.00Mercury1631Eng/L9621,0209915.85Molybdenum200.7ug/LNQ (40.0)NQ (40.0)NQ (40.0)NCNickel200.8ug/L8.807.408.1017.3Selenium200.8ug/L91.086.088.55.65Silver200.8ug/LND (2.00)ND (2.00)ND (2.00)NCSodium200.7ug/L30,00031,00030,5003.28Thallium200.8ug/LNQ (2.00)NQ (2.00)NQ (2.00)NCTin200.7ug/LND (200)ND (200)ND (200)NCTitanium200.7ug/LNQ (20.0)NQ (20.0)NQ (20.0)NCVanadium200.8ug/LND (10.0)ND (10.0)ND (10.0)NCZinc200.8ug/LNQ (10.0)NQ (10.0)NQ (10.0)NCDissolved MetalsAluminum200.7ug/LND (200)ND (200)ND (200)NCAntimony200.8ug/L2.102.402.2513.3Arsenic200.8ug/LNQ (4.00)NQ (4.00)NQ (4.00)NCBarium200.7ug/L2202202200.00Beryllium200.7ug/LND (2.00)ND (2.00)ND (2.00)NCBoron200.7ug/L59,000B58,000B58,5001.71Cadmium200.8ug/LND (4.00)ND (4.00)ND (4.00)NCCalcium200.7ug/L1,700,0001,700,0001,700,0000.00< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).NC – Not calculated.ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 5-7 5-7Table 5-1. Field Duplicate Results – Influent to Bioreactor System, AllenAnalyteUnitsMethodOriginal Influentto BioreactorSystem, Day 3Concentration (SP-2)Duplicate Influentto BioreactorSystemConcentration (SP-4)AverageResultRelativePercentDifferenceChromium200.8ug/LND (8.00)ND (8.00)ND (8.00)NCChromium (VI)218.6ug/LND (10.0)ND (10.0)ND (10.0)NCCobalt200.7ug/LNQ (20.0)NQ (20.0)NQ (20.0)NCCopper200.8ug/L11.011.011.00.00Iron200.7ug/LND (200)ND (200)ND (200)NCLead200.8ug/LND (2.00)ND (2.00)ND (2.00)NCMagnesium200.7ug/L420,000390,000405,0007.41Manganese200.7ug/L4204004104.88Mercury1631Eng/L3063013041.65Molybdenum200.7ug/LNQ (40.0)NQ (40.0)NQ (40.0)NCNickel200.8ug/L8.608.008.307.23Selenium200.8ug/L86.087.086.51.16Silver200.8ug/LND (2.00)ND (2.00)ND (2.00)NCSodium200.7ug/L32,00032,00032,0000.00Thallium200.8ug/LNQ (2.00)NQ (2.00)NQ (2.00)NCTin200.7ug/LND (200)ND (200)ND (200)NCTitanium200.7ug/LNQ (20.0)NQ (20.0)NQ (20.0)NCVanadium200.8ug/LND (10.0)NQ (10.0)NQ (10.0)NCZinc200.8ug/LNQ (10.0)NQ (10.0)NQ (10.0)NC< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).NC – Not calculated.ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 5-8Table 5-2. Field Duplicate Results – Effluent from Bioreactor System, AllenAnalyteUnitsMethodOriginal Effluentfrom BioreactorSystem, Day 2Concentration (SP-3)DuplicateEffluent fromBioreactorSystemConcentration (SP-5)AverageResultRelativePercentDifferenceClassicalsAmmonia350.1mg/L11.011.011.00.00Nitrate Nitrite as N353.2mg/L0.1300.1300.1300.00Nitrogen, Total Kjeldahl351.2mg/L12.013.012.58.00Biochemical Oxygen Demand5210Bmg/LNQ (2.50)NQ (2.50)NQ (2.50)NCChemical Oxygen Demand410.4mg/L79.055.067.035.8Chloride300.0mg/L3,2003,3003,2503.08Sulfate300.0mg/L1,5001,5001,5000.00Cyanide, Total4500 CN Emg/L0.110NQ (0.100)< 0.0800NCTotal Dissolved Solids2540Cmg/L8,1008,7008,4007.14Total Suspended Solids2540Dmg/LNQ (4.00)NQ (4.00)NQ (4.00)NCPhosphorus, Total365.1mg/L0.1600.1600.1600.00Total MetalsAluminum200.7ug/LNQ (200)NQ (200)NQ (200)NCAntimony200.8ug/LND (2.00)ND (2.00)ND (2.00)NCArsenic200.8ug/LNQ (4.00)NQ (4.00)NQ (4.00)NCBarium200.7ug/L2202102154.65Beryllium200.7ug/LND (2.00)ND (2.00)ND (2.00)NCBoron200.7ug/L66,000B66,000B66,0000.00Cadmium200.8ug/LND (4.00)ND (4.00)ND (4.00)NCCalcium200.7ug/L1,700,0001,600,0001,650,0006.06Chromium200.8ug/LND (8.00)^ND (8.00)^ND (8.00)NCCobalt200.7ug/LND (20.0)ND (20.0)ND (20.0)NC< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).NC – Not calculated.ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 5-9Table 5-2. Field Duplicate Results – Effluent from Bioreactor System, AllenAnalyteUnitsMethodOriginal Effluentfrom BioreactorSystem, Day 2Concentration (SP-3)DuplicateEffluent fromBioreactorSystemConcentration (SP-5)AverageResultRelativePercentDifferenceCopper200.8ug/LND (4.00)ND (4.00)ND (4.00)NCIron200.7ug/LNQ (200)NQ (200)NQ (200)NCLead200.8ug/LND (2.00)ND (2.00)ND (2.00)NCMagnesium200.7ug/L440,000430,000435,0002.30Manganese200.7ug/L4404304352.30Mercury1631Eng/L28.431.530.010.4Molybdenum200.7ug/LNQ (40.0)NQ (40.0)NQ (40.0)NCNickel200.8ug/LND (4.00)NQ (4.00)NQ (4.00)NCSelenium200.8ug/LNQ (4.00)NQ (4.00)NQ (4.00)NCSilver200.8ug/LND (2.00)ND (2.00)ND (2.00)NCSodium200.7ug/L33,00032,00032,5003.08Thallium200.8ug/LND (2.00)ND (2.00)ND (2.00)NCTin200.7ug/LND (200)ND (200)ND (200)NCTitanium200.7ug/LNQ (20.0)NQ (20.0)NQ (20.0)NCVanadium200.8ug/LND (10.0)ND (10.0)ND (10.0)NCZinc200.8ug/LNQ (10.0)NQ (10.0)NQ (10.0)NCDissolved MetalsAluminum200.7ug/LNQ (200)NQ (200)NQ (200)NCAntimony200.8ug/LND (2.00)ND (2.00)ND (2.00)NCArsenic200.8ug/LNQ (4.00)NQ (4.00)NQ (4.00)NCBarium200.7ug/L2202202200.00Beryllium200.7ug/LND (2.00)ND (2.00)ND (2.00)NCBoron200.7ug/L66,000B67,000B66,5001.50< - Average result includes at least one value measured belowthe quantitation limit. (Calculation uses 1⁄2 the quantitation limit for values below the quantitation limit).NC – Not calculated.ND – Not detected (number in parentheses is the quantitation limit).NQ – Analyte was measured above the method detection limit, butbelow the quantitation limit (number shown in parentheses is the quantitation limit). The analytical result ispresented in Appendix A with a J flag designation. A “NQ” for the 4-day average result indicates that all results were below the quantitation limit, but at least one result is a Jvalue.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.



# 5-10Table 5-2. Field Duplicate Results – Effluent from Bioreactor System, AllenAnalyteUnitsMethodOriginal Effluentfrom BioreactorSystem, Day 2Concentration (SP-3)DuplicateEffluent fromBioreactorSystemConcentration (SP-5)AverageResultRelativePercentDifferenceCadmium200.8ug/LND (4.00)ND (4.00)ND (4.00)NCCalcium200.7ug/L1,700,0001,700,0001,700,0000.00Chromium200.8ug/LND (8.00)ND (8.00)ND (8.00)NCChromium (VI)218.6ug/LND (2.00)ND (2.00)ND (2.00)NCCobalt200.7ug/LND (20.0)ND (20.0)ND (20.0)NCCopper200.8ug/LND (4.00)ND (4.00)ND (4.00)NCIron200.7ug/LNQ (200)BNQ (200)BNQ (200)NCLead200.8ug/LNQ (2.00)ND (2.00)NQ (2.00)NCMagnesium200.7ug/L430,000420,000425,0002.35Manganese200.7ug/L4104004052.47Mercury1631Eng/L33.133.733.41.80Molybdenum200.7ug/LND (40.0)ND (40.0)ND (40.0)NCNickel200.8ug/LNQ (4.00)ND (4.00)NQ (4.00)NCSelenium200.8ug/LNQ (4.00)NQ (4.00)NQ (4.00)NCSilver200.8ug/LND (2.00)ND (2.00)ND (2.00)NCSodium200.7ug/L34,00033,00033,5002.99Thallium200.8ug/LND (2.00)ND (2.00)ND (2.00)NCTin200.7ug/LND (200)ND (200)ND (200)NCTitanium200.7ug/LNQ (20.0)NQ (20.0)NQ (20.0)NCVanadium200.8ug/LND (10.0)ND (10.0)ND (10.0)NCZinc200.8ug/LNQ (10.0)NQ (10.0)NQ (10.0)NC

# 6-1 6. R EFERENCES



# 1. Eastern Research Group, Inc., 2010. Sampling Plan, Duke Energy Carolinas’ Allen

Steam Station. July 1. DCN SE00501.

2. Eastern Research Group, Inc., 2010. Quality Assurance Project Plan for the Steam

Electric Effluent Guidelines Rulemaking . (October). DCN SE00058.

# Appendix A

ANALYTICAL RESULTSND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-1Table A-1. Influent to FGD Wastewater Treatment System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L10.1111.4Nitrate Nitrite as N353.2mg/L20.0380.214Nitrogen, Total Kjeldahl351.2mg/L10.38516Biochemical Oxygen Demand5210Bmg/L12.82440BChemical Oxygen Demand410.4mg/L520100110*Chloride300.0mg/L200516002,800Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.25Total Dissolved Solids2540Cmg/L1471007,000Total Suspended Solids2540Dmg/L1281001,800Phosphorus, Total365.1mg/L10.0050.050.35Total MetalsAluminum200.7ug/L29.420070,000Antimony200.8ug/L20.7229.3Arsenic200.8ug/L21.24120Barium200.7ug/L22.520750Beryllium200.7ug/L20.71210Boron200.7ug/L10331,00054,000BCadmium200.8ug/L21.241.9JCalcium200.7ug/L103402,0001,900,000Chromium200.8ug/L23.38120^Cobalt200.7ug/L21.12052Copper200.8ug/L20.964140Iron200.7ug/L23220064,000Lead200.8ug/L20.73285Magnesium200.7ug/L221400440,000Manganese200.7ug/L20.51203,300ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-2Table A-1. Influent to FGD Wastewater Treatment System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMercury1631Eng/L4,0009652,02050,300Molybdenum200.7ug/L21.64032JNickel200.8ug/L20.894160Selenium200.8ug/L20.6241,500Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00029,000Thallium200.8ug/L20.4722.5Tin200.7ug/L24.82008.9JTitanium200.7ug/L21.6201,400Vanadium200.8ug/L21.710130Zinc200.8ug/L24.110220Dissolved MetalsAluminum200.7ug/L29.420024JAntimony200.8ug/L20.7222Arsenic200.8ug/L21.248.7Barium200.7ug/L22.520250Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00050,000BCadmium200.8ug/L21.241.5JCalcium200.7ug/L501,70010,0001,500,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)UCobalt200.7ug/L21.12017JCopper200.8ug/L20.96412Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400400,000Manganese200.7ug/L20.51202,800ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-3Table A-1. Influent to FGD Wastewater Treatment System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMercury1631Eng/L102.415.0515.2Molybdenum200.7ug/L21.64013JNickel200.8ug/L20.89471Selenium200.8ug/L20.624230Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00028,000Thallium200.8ug/L20.4720.93JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6202.5JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.11030ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-4Table A-2. Influent to FGD Wastewater Treatment System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L10.1117.3Nitrate Nitrite as N353.2mg/L20.0380.217Nitrogen, Total Kjeldahl351.2mg/L10.38521Biochemical Oxygen Demand5210Bmg/L10.32.59.7BChemical Oxygen Demand410.4mg/L1041200130J,*Chloride300.0mg/L200516003,400Sulfate300.0mg/L50122501,300Cyanide, Total4500 CN Emg/L10.020.10.34Total Dissolved Solids2540Cmg/L119408,900Total Suspended Solids2540Dmg/L1281003,900Phosphorus, Total365.1mg/L10.0050.050.015JTotal MetalsAluminum200.7ug/L29.420076,000Antimony200.8ug/L20.72211Arsenic200.8ug/L21.24140Barium200.7ug/L22.520880Beryllium200.7ug/L20.71212Boron200.7ug/L10331,00062,000BCadmium200.8ug/L21.242.9JCalcium200.7ug/L103402,0002,300,000Chromium200.8ug/L23.38140^Cobalt200.7ug/L21.12059Copper200.8ug/L20.964160Iron200.7ug/L23220069,000Lead200.8ug/L20.73299Magnesium200.7ug/L221400470,000Manganese200.7ug/L20.51203,700ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-5Table A-2. Influent to FGD Wastewater Treatment System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMercury1631Eng/L4,0009652,02049,600Molybdenum200.7ug/L21.64034JNickel200.8ug/L20.894190Selenium200.8ug/L20.6241,700Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00032,000Thallium200.8ug/L20.4723.2Tin200.7ug/L24.82008.9JTitanium200.7ug/L21.6201,500Vanadium200.8ug/L21.710150Zinc200.8ug/L24.110280Dissolved MetalsAluminum200.7ug/L29.420029JAntimony200.8ug/L20.7222.8Arsenic200.8ug/L21.2410Barium200.7ug/L22.520310Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00059,000BCadmium200.8ug/L21.241.6JCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)UCobalt200.7ug/L21.12020Copper200.8ug/L20.96412Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400440,000Manganese200.7ug/L20.51203,100ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-6Table A-2. Influent to FGD Wastewater Treatment System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMercury1631Eng/L204.8310.121Molybdenum200.7ug/L21.64018JNickel200.8ug/L20.89477Selenium200.8ug/L20.624290Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.4721.3JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.1JVanadium200.8ug/L21.7102JZinc200.8ug/L24.11043ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-7Table A-3. Influent to FGD Wastewater Treatment System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L20.11214Nitrate Nitrite as N353.2mg/L50.0950.520Nitrogen, Total Kjeldahl351.2mg/L41.52026Biochemical Oxygen Demand5210Bmg/L10.32.59.4Chemical Oxygen Demand410.4mg/L28.140130Chloride300.0mg/L200516004,200Sulfate300.0mg/L50122501,300Cyanide, Total4500 CN Emg/L10.020.10.19Total Dissolved Solids2540Cmg/L14710011,000Total Suspended Solids2540Dmg/L1281002,000Phosphorus, Total365.1mg/L10.0050.050.024JTotal MetalsAluminum200.7ug/L29.420072,000Antimony200.8ug/L20.72211Arsenic200.8ug/L21.24140Barium200.7ug/L22.520930Beryllium200.7ug/L20.71212Boron200.7ug/L10331,00080,000BCadmium200.8ug/L21.243.2JCalcium200.7ug/L103402,0002,400,000Chromium200.8ug/L23.38130^Cobalt200.7ug/L21.12064Copper200.8ug/L20.964160Iron200.7ug/L23220068,000Lead200.8ug/L20.732100Magnesium200.7ug/L221400520,000Manganese200.7ug/L20.51204,100Mercury1631Eng/L4,0009652,02044,600ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-8Table A-3. Influent to FGD Wastewater Treatment System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.64035JNickel200.8ug/L20.894190Selenium200.8ug/L20.6241,800Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00034,000Thallium200.8ug/L20.4723.3Tin200.7ug/L24.82009.4JTitanium200.7ug/L21.6201,600Vanadium200.8ug/L21.710160Zinc200.8ug/L24.110280Dissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7223.5Arsenic200.8ug/L21.2410Barium200.7ug/L22.520410Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00080,000BCadmium200.8ug/L21.242JCalcium200.7ug/L501,70010,0002,000,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)Cobalt200.7ug/L21.12022Copper200.8ug/L20.96411Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400500,000Manganese200.7ug/L20.51203,600Mercury1631Eng/L204.8310.116.4Molybdenum200.7ug/L21.64019JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-9Table A-3. Influent to FGD Wastewater Treatment System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.89480Selenium200.8ug/L20.624340Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00036,000Thallium200.8ug/L20.4721.7JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.2JVanadium200.8ug/L21.7101.7JZinc200.8ug/L24.11032ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-10Table A-4. Influent to FGD Wastewater Treatment System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L10.05519Nitrate Nitrite as N353.2mg/L50.0950.522Nitrogen, Total Kjeldahl351.2mg/L41.52045Biochemical Oxygen Demand5210Bmg/L10.32.56.6Chemical Oxygen Demand410.4mg/L28.140140Chloride300.0mg/L200516005,200Sulfate300.0mg/L50122501,300Cyanide, Total4500 CN Emg/L10.020.10.24BTotal Dissolved Solids2540Cmg/L14710011,000Total Suspended Solids2540Dmg/L1281002,200Phosphorus, Total365.1mg/L10.0050.050.046JTotal MetalsAluminum200.7ug/L29.420071,000Antimony200.8ug/L20.72212Arsenic200.8ug/L21.24140Barium200.7ug/L22.520990Beryllium200.7ug/L20.71212Boron200.7ug/L10331,000100,000BCadmium200.8ug/L21.244Calcium200.7ug/L103402,0002,700,000Chromium200.8ug/L23.38140^Cobalt200.7ug/L21.12067Copper200.8ug/L20.964180Iron200.7ug/L23220070,000Lead200.8ug/L20.732120Magnesium200.7ug/L221400590,000Manganese200.7ug/L20.51204,600Mercury1631Eng/L4,0009652,02052,100ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-11Table A-4. Influent to FGD Wastewater Treatment System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.64041Nickel200.8ug/L20.894210Selenium200.8ug/L20.6241,800Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00038,000Thallium200.8ug/L20.4723.1Tin200.7ug/L24.82009JTitanium200.7ug/L21.6201,600Vanadium200.8ug/L21.710180Zinc200.8ug/L24.110330Dissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7224Arsenic200.8ug/L21.247.1Barium200.7ug/L22.520500Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,000100,000BCadmium200.8ug/L21.242.3JCalcium200.7ug/L501,70010,0002,500,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)Cobalt200.7ug/L21.12025Copper200.8ug/L20.9648.9Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400550,000Manganese200.7ug/L20.51204,000Mercury1631Eng/L204.8310.175Molybdenum200.7ug/L21.64023JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-12Table A-4. Influent to FGD Wastewater Treatment System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.89493Selenium200.8ug/L20.624400Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00040,000Thallium200.8ug/L20.4721.7JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204.1JVanadium200.8ug/L21.7102.3JZinc200.8ug/L24.11043ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-13Table A-5. Influent to Bioreactor System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L10.1115.8Nitrate Nitrite as N353.2mg/L20.0380.214Nitrogen, Total Kjeldahl351.2mg/L20.1527.7Biochemical Oxygen Demand5210Bmg/L10.32.57.4BChemical Oxygen Demand410.4mg/L104120096J,*Chloride300.0mg/L200516003,100Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.084JTotal Dissolved Solids2540Cmg/L119407,500Total Suspended Solids2540Dmg/L11.142.4JPhosphorus, Total365.1mg/L10.0050.050.061Total MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.2Arsenic200.8ug/L21.242.3JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00055,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,600,000Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.96410Iron200.7ug/L23220036JLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400420,000Manganese200.7ug/L20.5120400ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-14Table A-5. Influent to Bioreactor System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMercury1631Eng/L500121252711Molybdenum200.7ug/L21.64020JNickel200.8ug/L20.8948.2Selenium200.8ug/L20.624110Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.4720.78JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6205.3JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1105.3JDissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.4Arsenic200.8ug/L21.242.9JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00057,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.96410Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400410,000Manganese200.7ug/L20.5120370ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-15Table A-5. Influent to Bioreactor System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMercury1631Eng/L204.8310.1219Molybdenum200.7ug/L21.64018JNickel200.8ug/L20.8949.2Selenium200.8ug/L20.62498Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.4720.87JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6202.9JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.11012ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-16Table A-6. Influent to Bioreactor System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L10.05516.8Nitrate Nitrite as N353.2mg/L20.0380.213Nitrogen, Total Kjeldahl351.2mg/L20.1528.6Biochemical Oxygen Demand5210Bmg/L10.32.55.2Chemical Oxygen Demand410.4mg/L28.14079Chloride300.0mg/L200516003,200Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.082JTotal Dissolved Solids2540Cmg/L119407,800Total Suspended Solids2540Dmg/L11.141.2JPhosphorus, Total365.1mg/L10.0050.050.033JTotal MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.2Arsenic200.8ug/L21.242.2JBarium200.7ug/L22.520200Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00054,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,600,000Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.9649.3Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400400,000Manganese200.7ug/L20.5120340Mercury1631Eng/L10024.150.51,320ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-17Table A-6. Influent to Bioreactor System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.64019JNickel200.8ug/L20.8947.9Selenium200.8ug/L20.62494Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00029,000Thallium200.8ug/L20.4720.87JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204.5JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1104.9JDissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.3Arsenic200.8ug/L21.242.9JBarium200.7ug/L22.520210Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00056,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)UCobalt200.7ug/L21.1201.2JCopper200.8ug/L20.9648.9Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400370,000Manganese200.7ug/L20.5120320Mercury1631Eng/L10024.150.5483Molybdenum200.7ug/L21.64018JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-18Table A-6. Influent to Bioreactor System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.8947.4Selenium200.8ug/L20.62493Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.4720.91JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.1JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1108JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-19Table A-7. Influent to Bioreactor System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L10.05518.9Nitrate Nitrite as N353.2mg/L20.0380.213Nitrogen, Total Kjeldahl351.2mg/L20.15210Biochemical Oxygen Demand5210Bmg/L10.32.53.6Chemical Oxygen Demand410.4mg/L52010072JChloride300.0mg/L200516003,200Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.12BTotal Dissolved Solids2540Cmg/L119407,200Total Suspended Solids2540Dmg/L11.141.6JPhosphorus, Total365.1mg/L10.0050.050.027JTotal MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.3Arsenic200.8ug/L21.242.2JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00059,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,700,000Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.96411Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400430,000Manganese200.7ug/L20.5120440Mercury1631Eng/L10024.150.5962ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-20Table A-7. Influent to Bioreactor System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.64020JNickel200.8ug/L20.8948.8Selenium200.8ug/L20.62491Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00030,000Thallium200.8ug/L20.4720.96JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204.1JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1106.8JDissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.1Arsenic200.8ug/L21.242.4JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00059,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)Cobalt200.7ug/L21.1201.3JCopper200.8ug/L20.96411Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400420,000Manganese200.7ug/L20.5120420Mercury1631Eng/L500121252306Molybdenum200.7ug/L21.64019JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-21Table A-7. Influent to Bioreactor System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.8948.6Selenium200.8ug/L20.62486Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00032,000Thallium200.8ug/L20.4720.94JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.3JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1106.3JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.E – MS and/or MSD exceeded the calibration range.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-22Table A-8. Influent to Bioreactor System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L20.11211Nitrate Nitrite as N353.2mg/L20.0380.213E,FNitrogen, Total Kjeldahl351.2mg/L10.07713.2FBiochemical Oxygen Demand5210Bmg/L10.32.53.9Chemical Oxygen Demand410.4mg/L28.14084FChloride300.0mg/L200516003,600Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.11BTotal Dissolved Solids2540Cmg/L119407,800Total Suspended Solids2540Dmg/L11.141.6JPhosphorus, Total365.1mg/L10.0050.050.02JTotal MetalsAluminum200.7ug/L29.420013J,BAntimony200.8ug/L20.7222.9Arsenic200.8ug/L21.241.8JBarium200.7ug/L22.520250Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00064,000B,4Cadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,800,0004Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.96412Iron200.7ug/L23220046J,BLead200.8ug/L20.7321.3JMagnesium200.7ug/L221400420,0004ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.E – MS and/or MSD exceeded the calibration range.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-23Table A-8. Influent to Bioreactor System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationManganese200.7ug/L20.5120520BMercury1631Eng/L5001212521,120Molybdenum200.7ug/L21.64021JNickel200.8ug/L20.8947.9Selenium200.8ug/L20.62487Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.4721.1JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6205.7JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1109JDissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.6Arsenic200.8ug/L21.241.5JBarium200.7ug/L22.520250Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00063,000B,4Cadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,900,0004Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)UCobalt200.7ug/L21.1201.2JCopper200.8ug/L20.96412Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.E – MS and/or MSD exceeded the calibration range.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-24Table A-8. Influent to Bioreactor System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMagnesium200.7ug/L221400400,0004Manganese200.7ug/L20.5120470Mercury1631Eng/L500121252361Molybdenum200.7ug/L21.64018JNickel200.8ug/L20.8948.2Selenium200.8ug/L20.62487Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.4721JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.4JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1106.6JND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-25Table A-9. Effluent from Bioreactor System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L20.22213FNitrate Nitrite as N353.2mg/L10.0190.10.026JNitrogen, Total Kjeldahl351.2mg/L40.314144Biochemical Oxygen Demand5210Bmg/L10.32.51.7J,BChemical Oxygen Demand410.4mg/L1041200120J,*Chloride300.0mg/L200516003,400Sulfate300.0mg/L50122501,500Cyanide, Total4500 CN Emg/L10.020.10.11Total Dissolved Solids2540Cmg/L119408,000Total Suspended Solids2540Dmg/L11.141.6JPhosphorus, Total365.1mg/L10.0050.050.11Total MetalsAluminum200.7ug/L29.420039JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.24ND (4)UBarium200.7ug/L22.520210Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00065,000B,4Cadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,700,0004Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200160JLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400450,0004ND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-26Table A-9. Effluent from Bioreactor System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationManganese200.7ug/L20.5120470Mercury1631Eng/L204.8310.112.6Molybdenum200.7ug/L21.6404.6JNickel200.8ug/L20.8941.1JSelenium200.8ug/L20.6241.2JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00033,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.8JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1104.7JDissolved MetalsAluminum200.7ug/L29.420032JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.242JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00071,000B,4Cadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,900,0004Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200140J,BLead200.8ug/L20.732ND (2)UND – Not detected (number in parentheses is the quantitation limit).* - LCS or LCSD is outside the laboratory control limits.^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-27Table A-9. Effluent from Bioreactor System, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMagnesium200.7ug/L221400440,0004Manganese200.7ug/L20.5120440Mercury1631Eng/L204.8310.110.4Molybdenum200.7ug/L21.6403.2JNickel200.8ug/L20.8941.3JSelenium200.8ug/L20.6240.8JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00034,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.4JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1106JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-28Table A-10. Effluent from Bioreactor System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L20.11211Nitrate Nitrite as N353.2mg/L10.0190.10.13Nitrogen, Total Kjeldahl351.2mg/L40.31412Biochemical Oxygen Demand5210Bmg/L10.32.51.8JChemical Oxygen Demand410.4mg/L28.14079Chloride300.0mg/L200516003,200Sulfate300.0mg/L50122501,500Cyanide, Total4500 CN Emg/L10.020.10.11Total Dissolved Solids2540Cmg/L119408,100Total Suspended Solids2540Dmg/L11.141.2JPhosphorus, Total365.1mg/L10.0050.050.16Total MetalsAluminum200.7ug/L29.420038JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.241.7JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00066,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,700,000Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200190JLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400440,000Manganese200.7ug/L20.5120440Mercury1631Eng/L204.8310.128.4ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-29Table A-10. Effluent from Bioreactor System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.6402.1JNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.6241.7JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00033,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204.2JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1105.4JDissolved MetalsAluminum200.7ug/L29.420040JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.241.8JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00066,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200120J,BLead200.8ug/L20.7321JMagnesium200.7ug/L221400430,000Manganese200.7ug/L20.5120410Mercury1631Eng/L204.8310.133.1Molybdenum200.7ug/L21.640ND (40)UND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-30Table A-10. Effluent from Bioreactor System, Day 2 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.8941.4JSelenium200.8ug/L20.6241.3JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00034,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6202.8JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1107.3JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-31Table A-11. Effluent from Bioreactor System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L20.11211Nitrate Nitrite as N353.2mg/L10.0190.1ND (0.1)UNitrogen, Total Kjeldahl351.2mg/L40.31412Biochemical Oxygen Demand5210Bmg/L10.242ND (2)UChemical Oxygen Demand410.4mg/L52010046J,FChloride300.0mg/L200516003,200Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.12BTotal Dissolved Solids2540Cmg/L119407,100Total Suspended Solids2540Dmg/L11.142.8JPhosphorus, Total365.1mg/L10.0050.050.19FTotal MetalsAluminum200.7ug/L29.420041JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.241.3JBarium200.7ug/L22.520210Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00063,000B,4Cadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,600,0004Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200210Lead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400410,0004ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-32Table A-11. Effluent from Bioreactor System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationManganese200.7ug/L20.5120400Mercury1631Eng/L204.8310.127.7Molybdenum200.7ug/L21.6401.6JNickel200.8ug/L20.8940.92JSelenium200.8ug/L20.6241.3JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.11015Dissolved MetalsAluminum200.7ug/L29.420025JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.242.2JBarium200.7ug/L22.520210Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00063,000B,4Cadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,600,0004Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L23220039J,BLead200.8ug/L20.732ND (2)UND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.4 – MS and/or MSD did not meet laboratorycriteria, but sample concentration is fourtimes greater thanthe matrix spike amount, so control limits are not applicable.B – Analyte was found in the blank and sample.F – MS and/or MSD not within laboratory control limits.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-33Table A-11. Effluent from Bioreactor System, Day 3 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMagnesium200.7ug/L221400400,0004Manganese200.7ug/L20.5120380Mercury1631Eng/L102.415.0528.3Molybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.6241JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00032,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.2JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1109.3JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-34Table A-12. Effluent from Bioreactor System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L20.11212Nitrate Nitrite as N353.2mg/L10.0190.10.097JNitrogen, Total Kjeldahl351.2mg/L20.15213Biochemical Oxygen Demand5210Bmg/L10.32.52.2JChemical Oxygen Demand410.4mg/L14.12036Chloride300.0mg/L200516003,200Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.1BTotal Dissolved Solids2540Cmg/L119407,500Total Suspended Solids2540Dmg/L11.141.2JPhosphorus, Total365.1mg/L10.0050.050.15Total MetalsAluminum200.7ug/L29.420035J,BAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.241.6JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00061,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,600,000Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200140J,BLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400420,000Manganese200.7ug/L20.5120440BMercury1631Eng/L204.8310.117ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-35Table A-12. Effluent from Bioreactor System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.8941.2JSelenium200.8ug/L20.6241.4JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204.4JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1105.7JDissolved MetalsAluminum200.7ug/L14.710011JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.241.5JBarium200.7ug/L11.210110Beryllium200.7ug/L10.361ND (1)UBoron200.7ug/L501705,00057,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L10.5710ND (10)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L11610046J,BLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L111200190,000Manganese200.7ug/L10.2510190Mercury1631Eng/L204.8310.115.9Molybdenum200.7ug/L10.81202.8JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-36Table A-12. Effluent from Bioreactor System, Day 4 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.8941.1JSelenium200.8ug/L20.6241.2JSilver200.8ug/L20.592ND (2)USodium200.7ug/L1925,00016,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L12.4100ND (100)UTitanium200.7ug/L10.82101.6JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1105.6JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-37Table A-13. Duplicate of Influent to Bioreactor SystemAnalytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L10.05518.8Nitrate Nitrite as N353.2mg/L20.0380.213Nitrogen, Total Kjeldahl351.2mg/L20.15211Biochemical Oxygen Demand5210Bmg/L10.32.53.7Chemical Oxygen Demand410.4mg/L28.14094Chloride300.0mg/L200516003,400Sulfate300.0mg/L50122501,400Cyanide, Total4500 CN Emg/L10.020.10.14BTotal Dissolved Solids2540Cmg/L119407,100Total Suspended Solids2540Dmg/L11.141.2JPhosphorus, Total365.1mg/L10.0050.050.035JTotal MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.3Arsenic200.8ug/L21.241.4JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00059,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,700,000Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.96411Iron200.7ug/L23220034JLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400410,000Manganese200.7ug/L20.5120440Mercury1631Eng/L10024.150.51,020ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-38Table A-13. Duplicate of Influent to Bioreactor SystemAnalytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.64022JNickel200.8ug/L20.8947.4Selenium200.8ug/L20.62486Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00031,000Thallium200.8ug/L20.4721.1JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204.4JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1104.9JDissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.7222.4Arsenic200.8ug/L21.242.9JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00058,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L51.9610ND (10)UCobalt200.7ug/L21.1201.1JCopper200.8ug/L20.96411Iron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400390,000Manganese200.7ug/L20.5120400Mercury1631Eng/L500121252301Molybdenum200.7ug/L21.64019JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-39Table A-13. Duplicate of Influent to Bioreactor SystemAnalytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.8948Selenium200.8ug/L20.62487Silver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00032,000Thallium200.8ug/L20.4721.1JTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.3JVanadium200.8ug/L21.7101.7JZinc200.8ug/L24.1105.9JND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-40Table A-14. Duplicate of Effluent from Bioreactor System Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationClassicalsAmmonia350.1mg/L20.11211Nitrate Nitrite as N353.2mg/L10.0190.10.13Nitrogen, Total Kjeldahl351.2mg/L40.31413Biochemical Oxygen Demand5210Bmg/L10.32.51.9JChemical Oxygen Demand410.4mg/L28.14055Chloride300.0mg/L200516003,300Sulfate300.0mg/L50122501,500Cyanide, Total4500 CN Emg/L10.020.10.089JTotal Dissolved Solids2540Cmg/L119408,700Total Suspended Solids2540Dmg/L11.141.6JPhosphorus, Total365.1mg/L10.0050.050.16Total MetalsAluminum200.7ug/L29.420038JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.241.7JBarium200.7ug/L22.520210Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L10331,00066,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L103402,0001,600,000Chromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200190JLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400430,000Manganese200.7ug/L20.5120430Mercury1631Eng/L204.8310.131.5ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-41Table A-14. Duplicate of Effluent from Bioreactor System Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationMolybdenum200.7ug/L21.6402.4JNickel200.8ug/L20.8940.94JSelenium200.8ug/L20.6241.5JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00032,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6204.7JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1104.7JDissolved MetalsAluminum200.7ug/L29.420028JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.242JBarium200.7ug/L22.520220Beryllium200.7ug/L20.712ND (2)UBoron200.7ug/L501705,00067,000BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L501,70010,0001,700,000Chromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200110J,BLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400420,000Manganese200.7ug/L20.5120400Mercury1631Eng/L204.8310.133.7Molybdenum200.7ug/L21.640ND (40)UND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).



# A-42Table A-14. Duplicate of Effluent from Bioreactor System Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.6241JSilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,00033,000Thallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.6203.1JVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1104.8J

# a 

# Aluminum was detected in two equipment blanks for the 10-liter composite container at 2.53 ug/L and 4.71 ug/L.

ND – Not detected (number in parenthesis is the quantitation limit).



# A-43 Table A-15. Bottle Blank and Equipmen t Blank Analytical Data, Allen

Analyte Method Process Units Concentration

HDPE 250 ml Plastic Bottles – Bottle Blank (for metals and hexavalent chromium samples)

Aluminum EPA 1638 Total Recoverable ug/L ND (4.0)

Antimony EPA 1638 Total Recoverable ug/L ND (0.020)

Arsenic EPA 1638 Total Recoverable ug/L ND (0.15)

Barium EPA 1638 Total Recoverable ug/L ND (0.20)

Beryllium EPA 1638 Total Recoverable ug/L ND (0.060)

Boron EPA 1638 Total Recoverable ug/L ND (3.0)

Cadmium EPA 1638 Total Recoverable ug/L ND (0.020)

Calcium EPA 1638 Total Recoverable ug/L ND (40)

Chromium EPA 1638 Total Recoverable ug/L ND (0.10)

Cobalt EPA 1638 Total Recoverable ug/L ND (0.10)

Copper EPA 1638 Total Recoverable ug/L ND (0.10)

Iron EPA 1638 Total Recoverable ug/L ND (10)

Lead EPA 1638 Total Recoverable ug/L ND (0.040)

Magnesium EPA 1638 Total Recoverable ug/L ND (2.5)

Manganese EPA 1638 Total Recoverable ug/L ND (0.10)

Molybdenum EPA 1638 Total Recoverable ug/L ND (0.060)

Nickel EPA 1638 Total Recoverable ug/L ND (0.10)

Selenium EPA 1638 Total Recoverable ug/L ND (0.60)

Silver EPA 1638 Total Recoverable ug/L ND (0.020)

Sodium EPA 1638 Total Recoverable ug/L ND (20)

Thallium EPA 1638 Total Recoverable ug/L ND (0.0050)

Tin EPA 1638 Total Recoverable ug/L ND (0.10)

Titanium EPA 1638 Total Recoverable ug/L ND (0.50)

Vanadium EPA 1638 Total Recoverable ug/L ND (0.10)

Zinc EPA 1638 Total Recoverable ug/L ND (0.20)

HDPE 500 ml Plastic Bottles – Bottle Blank (for metals samples)

Aluminum EPA 1638 Total Recoverable ug/L ND (4.0)

Antimony EPA 1638 Total Recoverable ug/L ND (0.020)

Arsenic EPA 1638 Total Recoverable ug/L ND (0.15)

Barium EPA 1638 Total Recoverable ug/L ND (0.20)

Beryllium EPA 1638 Total Recoverable ug/L ND (0.060)

Boron EPA 1638 Total Recoverable ug/L ND (3.0)

Cadmium EPA 1638 Total Recoverable ug/L ND (0.020)

Calcium EPA 1638 Total Recoverable ug/L ND (40)

Chromium EPA 1638 Total Recoverable ug/L ND (0.10)

Cobalt EPA 1638 Total Recoverable ug/L ND (0.10)

Copper EPA 1638 Total Recoverable ug/L ND (0.10)

Iron EPA 1638 Total Recoverable ug/L ND (10)

# a 

# Aluminum was detected in two equipment blanks for the 10-liter composite container at 2.53 ug/L and 4.71 ug/L.

ND – Not detected (number in parenthesis is the quantitation limit).



# A-44 Table A-15. Bottle Blank and Equipmen t Blank Analytical Data, Allen

Analyte Method Process Units Concentration

Lead EPA 1638 Total Recoverable ug/L ND (0.040)

Magnesium EPA 1638 Total Recoverable ug/L ND (2.5)

Manganese EPA 1638 Total Recoverable ug/L ND (0.10)

Molybdenum EPA 1638 Total Recoverable ug/L ND (0.060)

Nickel EPA 1638 Total Recoverable ug/L ND (0.10)

Selenium EPA 1638 Total Recoverable ug/L ND (0.60)

Silver EPA 1638 Total Recoverable ug/L ND (0.020)

Sodium EPA 1638 Total Recoverable ug/L ND (20)

Thallium EPA 1638 Total Recoverable ug/L ND (0.0050)

Tin EPA 1638 Total Recoverable ug/L ND (0.10)

Titanium EPA 1638 Total Recoverable ug/L ND (0.50)

Vanadium EPA 1638 Total Recoverable ug/L ND (0.10)

Zinc EPA 1638 Total Recoverable ug/L ND (0.20)

125 ml Glass Bottles – Bottle Blank (for mercury composite samples)

Mercury EPA 1631E Total Recoverable ug/L ND (0.00050)

250 ml Glass Bottles – Bottle Bl ank (for mercury grab samples)

Mercury EPA 1631E Total Recoverable ug/L ND (0.00050)

LDPE 10 L Composite Bottles – Bottle Blank (sample composite bottle for dissolved/filtration pump-off)

Aluminum a 

# EPA 1638 Dissolved ug/L 4.71

Antimony EPA 1638 Dissolved ug/L ND (0.50)

Arsenic EPA 1638 Dissolved ug/L ND (0.50)

Barium EPA 1638 Dissolved ug/L ND (0.10)

Beryllium EPA 1638 Dissolved ug/L ND (0.10)

Cadmium EPA 1638 Dissolved ug/L ND (0.10)

Chromium EPA 1638 Dissolved ug/L ND (1.0)

Cobalt EPA 1638 Dissolved ug/L ND (0.10)

Copper EPA 1638 Dissolved ug/L ND (0.30)

Lead EPA 1638 Dissolved ug/L ND (0.050)

Manganese EPA 1638 Dissolved ug/L ND (0.50)

Molybdenum EPA 1638 Dissolved ug/L ND (0.50)

Nickel EPA 1638 Dissolved ug/L ND (0.50)

Selenium EPA 1638 Dissolved ug/L ND (2.0)

Silver EPA 1638 Dissolved ug/L ND (0.10)

Thallium EPA 1638 Dissolved ug/L ND (0.050)

Vanadium EPA 1638 Dissolved ug/L ND (1.0)

Zinc EPA 1638 Dissolved ug/L ND (0.50)

LDPE 20 L Composite Bottles – Bottle Blank (sample composite bottle for dissolved/filtration pump-off)

Aluminum EPA 1638 Dissolved ug/L ND (2.0)

Antimony EPA 1638 Dissolved ug/L ND (0.50)

# a 

# Aluminum was detected in two equipment blanks for the 10-liter composite container at 2.53 ug/L and 4.71 ug/L.

ND – Not detected (number in parenthesis is the quantitation limit).



# A-45 Table A-15. Bottle Blank and Equipmen t Blank Analytical Data, Allen

Analyte Method Process Units Concentration

Arsenic EPA 1638 Dissolved ug/L ND (0.50)

Barium EPA 1638 Dissolved ug/L ND (0.10)

Beryllium EPA 1638 Dissolved ug/L ND (0.10)

Cadmium EPA 1638 Dissolved ug/L ND (0.10)

Chromium EPA 1638 Dissolved ug/L ND (1.0)

Cobalt EPA 1638 Dissolved ug/L ND (0.10)

Copper EPA 1638 Dissolved ug/L ND (0.30)

Lead EPA 1638 Dissolved ug/L ND (0.050)

Manganese EPA 1638 Dissolved ug/L ND (0.50)

Molybdenum EPA 1638 Dissolved ug/L ND (0.50)

Nickel EPA 1638 Dissolved ug/L ND (0.50)

Selenium EPA 1638 Dissolved ug/L ND (2.0)

Silver EPA 1638 Dissolved ug/L ND (0.10)

Thallium EPA 1638 Dissolved ug/L ND (0.050)

Vanadium EPA 1638 Dissolved ug/L ND (1.0)

Zinc EPA 1638 Dissolved ug/L ND (0.50)

Sample Tap Tubing Set – Equipment Blank (seven feet of #73 silicone tubing, Y-sp litter, and two pieces of 5

feet of #24 silicone tubing for sample collect ion for mercury and metals from sample taps)

Aluminum EPA 1638 Total Recoverable ug/L ND (4.0)

Antimony EPA 1638 Total Recoverable ug/L ND (0.020)

Arsenic EPA 1638 Total Recoverable ug/L ND (0.15)

Barium EPA 1638 Total Recoverable ug/L ND (0.20)

Beryllium EPA 1638 Total Recoverable ug/L ND (0.060)

Boron EPA 1638 Total Recoverable ug/L ND (3.0)

Cadmium EPA 1638 Total Recoverable ug/L ND (0.020)

Calcium EPA 1638 Total Recoverable ug/L ND (40)

Chromium EPA 1638 Total Recoverable ug/L ND (0.10)

Cobalt EPA 1638 Total Recoverable ug/L ND (0.10)

Copper EPA 1638 Total Recoverable ug/L ND (0.10)

Iron EPA 1638 Total Recoverable ug/L ND (10)

Lead EPA 1638 Total Recoverable ug/L ND (0.040)

Magnesium EPA 1638 Total Recoverable ug/L ND (2.5)

Manganese EPA 1638 Total Recoverable ug/L ND (0.10)

Mercury EPA 1631E Total Recoverable ug/L ND (0.00050)

Molybdenum EPA 1638 Total Recoverable ug/L ND (0.060)

Nickel EPA 1638 Total Recoverable ug/L ND (0.10)

Selenium EPA 1638 Total Recoverable ug/L ND (0.60)

Silver EPA 1638 Total Recoverable ug/L ND (0.020)

Sodium EPA 1638 Total Recoverable ug/L ND (20)

# a 

# Aluminum was detected in two equipment blanks for the 10-liter composite container at 2.53 ug/L and 4.71 ug/L.

ND – Not detected (number in parenthesis is the quantitation limit).



# A-46 Table A-15. Bottle Blank and Equipmen t Blank Analytical Data, Allen

Analyte Method Process Units Concentration

Thallium EPA 1638 Total Recoverable ug/L ND (0.0050)

Tin EPA 1638 Total Recoverable ug/L ND (0.10)

Titanium EPA 1638 Total Recoverable ug/L ND (0.50)

Vanadium EPA 1638 Total Recoverable ug/L ND (0.10)

Zinc EPA 1638 Total Recoverable ug/L ND (0.20)

Pump-Off Tubing Set – Equipment Blan k (Three feet of Teflon® tubing, 10 feet of #73 silicone tubing, Ysplitter, and two pieces of 5 feet of #24 silicone tubing for total and di ssolved sample collection for metals)

Aluminum EPA 1638 Total Recoverable ug/L ND (4.0)

Antimony EPA 1638 Total Recoverable ug/L ND (0.020)

Arsenic EPA 1638 Total Recoverable ug/L ND (0.15)

Barium EPA 1638 Total Recoverable ug/L ND (0.20)

Beryllium EPA 1638 Total Recoverable ug/L ND (0.060)

Boron EPA 1638 Total Recoverable ug/L ND (3.0)

Cadmium EPA 1638 Total Recoverable ug/L ND (0.020)

Calcium EPA 1638 Total Recoverable ug/L ND (40)

Chromium EPA 1638 Total Recoverable ug/L ND (0.10)

Cobalt EPA 1638 Total Recoverable ug/L ND (0.10)

Copper EPA 1638 Total Recoverable ug/L ND (0.10)

Iron EPA 1638 Total Recoverable ug/L ND (10)

Lead EPA 1638 Total Recoverable ug/L ND (0.040)

Magnesium EPA 1638 Total Recoverable ug/L ND (2.5)

Manganese EPA 1638 Total Recoverable ug/L ND (0.10)

Mercury EPA 1631E Total Recoverable ug/L ND (0.00050)

Molybdenum EPA 1638 Total Recoverable ug/L ND (0.060)

Nickel EPA 1638 Total Recoverable ug/L ND (0.10)

Selenium EPA 1638 Total Recoverable ug/L ND (0.60)

Silver EPA 1638 Total Recoverable ug/L ND (0.020)

Sodium EPA 1638 Total Recoverable ug/L ND (20)

Thallium EPA 1638 Total Recoverable ug/L ND (0.0050)

Tin EPA 1638 Total Recoverable ug/L ND (0.10)

Titanium EPA 1638 Total Recoverable ug/L ND (0.50)

Vanadium EPA 1638 Total Recoverable ug/L ND (0.10)

Zinc EPA 1638 Total Recoverable ug/L ND (0.20)

T-Splitter Tubing Set – Equipment Blank (Ten feet of #7 3 silicone tubing, T-splitter, six feet of #73 silicone

tubing, and 15 feet of #73 silicone tubing for total and dissolved sample collect ion for metal composites)

Aluminum EPA 1638 Total Recoverable ug/L ND (4.0)

Antimony EPA 1638 Total Recoverable ug/L ND (0.020)

Arsenic EPA 1638 Total Recoverable ug/L ND (0.15)

Barium EPA 1638 Total Recoverable ug/L ND (0.20)

Beryllium EPA 1638 Total Recoverable ug/L ND (0.060)

# a 

# Aluminum was detected in two equipment blanks for the 10-liter composite container at 2.53 ug/L and 4.71 ug/L.

ND – Not detected (number in parenthesis is the quantitation limit).



# A-47 Table A-15. Bottle Blank and Equipmen t Blank Analytical Data, Allen

Analyte Method Process Units Concentration

Boron EPA 1638 Total Recoverable ug/L ND (3.0)

Cadmium EPA 1638 Total Recoverable ug/L ND (0.020)

Calcium EPA 1638 Total Recoverable ug/L ND (40)

Chromium EPA 1638 Total Recoverable ug/L ND (0.10)

Cobalt EPA 1638 Total Recoverable ug/L ND (0.10)

Copper EPA 1638 Total Recoverable ug/L ND (0.10)

Iron EPA 1638 Total Recoverable ug/L ND (10)

Lead EPA 1638 Total Recoverable ug/L ND (0.040)

Magnesium EPA 1638 Total Recoverable ug/L ND (2.5)

Manganese EPA 1638 Total Recoverable ug/L ND (0.10)

Mercury EPA 1631E Total Recoverable ug/L ND (0.00050)

Molybdenum EPA 1638 Total Recoverable ug/L ND (0.060)

Nickel EPA 1638 Total Recoverable ug/L ND (0.10)

Selenium EPA 1638 Total Recoverable ug/L ND (0.60)

Silver EPA 1638 Total Recoverable ug/L ND (0.020)

Sodium EPA 1638 Total Recoverable ug/L ND (20)

Thallium EPA 1638 Total Recoverable ug/L ND (0.0050)

Tin EPA 1638 Total Recoverable ug/L ND (0.10)

Titanium EPA 1638 Total Recoverable ug/L ND (0.50)

Vanadium EPA 1638 Total Recoverable ug/L ND (0.10)

Zinc EPA 1638 Total Recoverable ug/L ND (0.20)

# A-48ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-16. Influent to FGD Wastewater Treatment System Field Blank, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsAluminum200.7ug/L29.420018JAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.24ND (4)UBarium200.7ug/L22.520ND (20)UBeryllium200.7ug/L20.712ND (2)UBoron200.7ug/L26.720043J,BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L26940097JChromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L23220092JLead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400ND (400)UManganese200.7ug/L20.51201.2JMercury1631Eng/L10.240.5ND (0.5)UMolybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.624ND (4)USilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,000ND (10,000)UThallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.620ND (20)UVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.110ND (10)U

# A-49ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-16. Influent to FGD Wastewater Treatment System Field Blank, Day 1 Analytical Results, AllenAnalyteUnitsMethodDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationDissolved MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.24ND (4)UBarium200.7ug/L22.520ND (20)UBeryllium200.7ug/L20.712ND (2)UBoron200.7ug/L26.72009J,BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L269400ND (400)UChromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.9642.1JIron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400ND (400)UManganese200.7ug/L20.5120ND (20)UMolybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.624ND (4)USilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,000ND (10,000)UThallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.620ND (20)UVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1108J

# A-50ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-17. Influent to Bioreactor System Field Blank, Day1 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.24ND (4)UBarium200.7ug/L22.520ND (20)UBeryllium200.7ug/L20.712ND (2)UBoron200.7ug/L26.720039J,BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L269400ND (400)UChromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400ND (400)UManganese200.7ug/L20.5120ND (20)UMercury1631Eng/L10.240.5ND (0.5)UMolybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.624ND (4)USilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,000ND (10,000)UThallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.620ND (20)UVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1106.9JDissolved MetalsAluminum200.7ug/L29.4200ND (200)U

# A-51ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-17. Influent to Bioreactor System Field Blank, Day1 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.24ND (4)UBarium200.7ug/L22.520ND (20)UBeryllium200.7ug/L20.712ND (2)UBoron200.7ug/L26.7200ND (200)UCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L269400ND (400)UChromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400ND (400)UManganese200.7ug/L20.5120ND (20)UMolybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.624ND (4)USilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,000ND (10,000)UThallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.620ND (20)UVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1107J

# A-52ND – Not detected (number in parentheses is the quantitation limit).U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-18. Influent to Bioreactor System Field Blank, Day2 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsMercury1631Eng/L10.240.5ND (0.5)U

# A-53ND – Not detected (number in parentheses is the quantitation limit).U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-19. Influent to Bioreactor System Field Blank, Day3 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsMercury1631Eng/L10.240.5ND (0.5)U

# A-54ND – Not detected (number in parentheses is the quantitation limit).U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-20. Influent to Bioreactor System Field Blank, Day4 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsMercury1631Eng/L10.240.5ND (0.5)U

# A-55ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-21. Effluent from Bioreactor System Field Blank, Day 1 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsAluminum200.7ug/L29.4200ND (200)UAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.24ND (4)UBarium200.7ug/L22.520ND (20)UBeryllium200.7ug/L20.712ND (2)UBoron200.7ug/L26.720032J,BCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L269400ND (400)UChromium200.8ug/L23.38ND (8)U,^Cobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400ND (400)UManganese200.7ug/L20.5120ND (20)UMercury1631Eng/L10.240.5ND (0.5)UMolybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.624ND (4)USilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,000ND (10,000)UThallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.620ND (20)UVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1106.1JDissolved MetalsAluminum200.7ug/L29.4200ND (200)U

# A-56ND – Not detected (number in parentheses is the quantitation limit).^ - ICV, CCV, ICB, CCB, ISA, ISB, CRI, or MRL standard: Instrument related QC exceeds the control limits.B – Analyte was found in the blank and sample.J – Result measured above the MDL, but less than the quantitation limit.U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-21. Effluent from Bioreactor System Field Blank, Day 1 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationAntimony200.8ug/L20.722ND (2)UArsenic200.8ug/L21.24ND (4)UBarium200.7ug/L22.520ND (20)UBeryllium200.7ug/L20.712ND (2)UBoron200.7ug/L26.7200ND (200)UCadmium200.8ug/L21.24ND (4)UCalcium200.7ug/L269400ND (400)UChromium200.8ug/L23.38ND (8)UChromium (VI)218.6ug/L10.392ND (2)UCobalt200.7ug/L21.120ND (20)UCopper200.8ug/L20.964ND (4)UIron200.7ug/L232200ND (200)ULead200.8ug/L20.732ND (2)UMagnesium200.7ug/L221400ND (400)UManganese200.7ug/L20.5120ND (20)UMolybdenum200.7ug/L21.640ND (40)UNickel200.8ug/L20.894ND (4)USelenium200.8ug/L20.624ND (4)USilver200.8ug/L20.592ND (2)USodium200.7ug/L218010,000ND (10,000)UThallium200.8ug/L20.472ND (2)UTin200.7ug/L24.8200ND (200)UTitanium200.7ug/L21.620ND (20)UVanadium200.8ug/L21.710ND (10)UZinc200.8ug/L24.1109.5J

# A-57ND – Not detected (number in parentheses is the quantitation limit).U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-22. Effluent from Bioreactor System Field Blank, Day 2 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsMercury1631Eng/L10.240.5ND (0.5)U

# A-58ND – Not detected (number in parentheses is the quantitation limit).U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-23. Effluent from Bioreactor System Field Blank, Day 3 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsMercury1631Eng/L10.240.5ND (0.5)U

# A-59ND – Not detected (number in parentheses is the quantitation limit).U – Result below the MDL. (Number shown in parentheses is the quantitation limit).Table A-24. Effluent from Bioreactor System Field Blank, Day 4 Analytical Results, AllenAnalyteMethodUnitsDilutionFactorSampleSpecific MDLSampleSpecific QLConcentrationTotal MetalsMercury1631Eng/L10.240.5ND (0.5)U

# Appendix B

DATA REVIEW NARRATIVES

# Appendix C

PICTURES FROM THE SAMPLING EPISODE

# C-1 Figure C-1. Sampler Working Inside a “Cleanbox”

# C-2 Figure C-2. Sample Tap and Tubing at the Influent to the FGD Wastewater Treatment

System

# C-3 Figure C-3. Sample Collection Set-Up at the Pump-Off Station

# C-4 Figure C-4. Sample Collection Set-Up at th e Influent to the Bioreactor System

# C-5 Figure C-5. Sample Tap and Tubing at the Effluent from the Bioreactor System