### SAT Report PMN Number: P-15-0025 SAT Date: 10/21/2014

Print Date: 2/3/2015

Related cases : CBI : Yes Health related cases:

Concern levels : Type of Concern: Health Eco Comments Level of Concern: 1-2 3 Health: ; Eco: chronic concerns only

Persistence Bioaccum Toxicity Comments 1 21

Exposure Based Review : Health: N o Ecotox: Yes
Routes of exposure : Health: Dermal Inhalation Ecotox: All releases to water Fate: ; Keywords : Keywords: IRR-S,E,MM,L; SENS-S (UNCERT); AQUATOX
Summary of Assessment :

Fate : Fate Summary: P-15-0025 FATE: Liquid with MP < 25 C (E) log Kow = 7.49 (E) S = 0.005 mg/L at 25 C (E) VP = 2.8E-4 torr at 25 C (E) BP = 337 C (E) H = 1.17E-2 (E) log Koc = 4.65 (E) log Fish BCF = 3.29 (E)log Fish BAF = 4.25 (E) POTW removal (%) = 90 via sorption and possible biodeg; Analog: OECD 301D(Closed Btl): 85%/28d. Time for complete ultimate aerobic biodeg = wk-mo Sorption to soils/sediments = strong Volatilization half-life from a standard river = 2 hrs Volatilization half-life from a standard lake = 6 da Atmospheric Oxidation Half-life = 6 hr via OH radical PBT Potential: P1B2 *CEB FATE: Migration to ground water = slow

Health : Health Summary: Absorption is poor all routes ( p che m ). Concern for irritation to all tissues and uncertain concern for dermal sensitization based on test data for

Ecotox :
Test Organism Test Type Test End Point Predicted Measured Comments

fish 96-h LC50 * daphnid 48-h LC50 * 0.030 green algal 96-h EC50 * fish

_ chronic value 0.001 daphnid

_ chronic value 0.001 0.003

algal

_ chronic value 0.014

Sewage Sludge 3-h EC50

_ Sewage Sludge

_ Chronic Value _

Ecotox Values Comments: Predictions are based on ; liquid with mp unknown (P); log Kow = 7.34 (EPI); S = 0.005 mg/L at 25 C (P); p H7; effective concentrations based on 100% active ingredients and mean measured concentrations; hardness < 150.0 mg/L as CaCO3; and TOC <2.0 mg/L;
Ecotoxicity Test Data Results for P-15-0025:
An acute daphnid toxicity study was conducted on an analog of the PMN (P-15-0025). The analog was ; . EPA classified the PMN and the analog as a . Based on structural depictions, the difference between the analog and the PMN is the presence of a double bond that is not nearby the group (i.e., not in the alpha or beta position) and minimal branching, which makes it a good analog. The PMN was predicted to have a water solubility of 0.005 mg/L and a Log Kow of 7.5.Daphnid Toxicity Summary: conducted a 48-hour acute immobilization test in Daphnia magna with an analog test substance (101.1% (w/w) active content ) under semi-static conditions with 24-hour renewal. This study was reported to follow OECD test guideline No. 202 (2004). Following a range-finding test, four replicates of five D. magna were exposed to a dilution water control (filtered natural river water) or the test substance prepared as a water soluble fraction (WSF) at nominal loading levels of 0.032, 0.1, 0.32, 1.0, 3.2 or 10.0 mg/L. Corresponding geometric mean initially measured concentrations were 0.0160, 0.0569, 0.155, 0.542, 1.90 and 5.91 mg/L (48

–59% of nominal), as determined by GC-MS analysis (LOQ = 2.0 μg/L, enrichment factor taken into account). EPA prefers analytically confirmed test concentrations at study termination for all exposures, but the submitter demonstrated that loss was minimal during the renewal period. The nominal 10 mg/L WSF was prepared by shaking with 20 rpm for 24 hours at room temperature, followed by a separation phase of at least 24 hours. Afterwards, the WSF was taken from the homogenous liquid phase. N o filtration was applied to prepare the WSF. The 10 mg/L WSF was diluted to prepare the desired nominal loading levels. The concentrations of test substance were analyzed at all concentration levels from freshly prepared media and at two concentration levels (nominal 0.1 and 1.0 mg/L) from old media. Recoveries of fresh media ranged from 44

–62% of nominal values; recoveries of old media ranged from 34

–66% of nominal values. Over the course of testing, temperature was maintained at 18

–22°C, ±1°C, p H ranged fro m 7.53

–7.87 and dissolved oxygen ranged from 8.24

–9.63 mg/L. Dilution water hardness was 154 mg CaCO-3/L; was 3.9 mg/L. A loading rate of 250 daphnids/L was calculated, which is considered high and may result in crowding. The % immobilization at geometric mean initially measured concentrations of 0 (control), 0.0160, 0.0569, 0.155, 0.542, 1.90 and 5.91 mg/L was 0, 10, 90, 100, 100, 100 and 100%, respectively at 48 hours. Based on geometric mean initially measured concentrations, the 48-hour EC50 was 0.0302 mg/L. Each test concentration is supposed to be prepared independently for water soluble fractions; however, analytic monitoring confirmed test concentrations. 48-hour EC50 = 0.0302 mg/L
Conclusion: The acute daphnid study was acceptable. N o fish and algae data were provided. An acute of 0.006 mg/L (6 p pb) was concluded from the experimental daphnid 48-hour EC50 of 0.0302 mg/L with application of an assessment factor of 5 to account for species sensitivity distributions. A chronic of 0.001 mg/L (1 p pb) was concluded based on the predicted fish ChV of 0.001 mg/L and application of an assessment factor of 10 to account for species sensitivity distributions.

Acute : 6 p pb Chronic : 1 p pb

Reviewer: K.Moran

Factors Values Comments Assessment Factor 10 Concentration of Concern ( p pb) 1SARs SAR Class Ecotox Category

Ecotox Factors Comments:

SAT Chair: L Keifer 564-8916