# Patent

## Title
Control of AAD-1 monocot volunteers in fields of dicot crops

## Abstract

The subject invention relates in part to the control of AAD-1 monocot volunteers in fields planted with dicot crops such as soybeans or cotton. According to some embodiments of the subject invention, cyclohexanedione herbicides are selected as being an effective tool for controlling AAD-1 volunteers, as AAD genes do not impart tolerance to this class of graminicide chemistry. In addition, imidazolinone-class herbicides can be used in some preferred embodiments for selective control of conventional or herbicide-tolerant varieties of volunteer corn. AAD-1 corn comprising Event DAS-40278-9 is used in some particularly preferred embodiments.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a 371 national stage application of International Application No. PCT/US2010/045870, with an International Filing Date of Aug. 18, 2010, which claims priority to U.S. Application No. 61/235,248, filed on Aug. 19,2009, and to U.S. Application No. 61/328,944, filed on Aug. 28, 2010, all of which are incorporated herein by reference in their entirety.


BACKGROUND
Corn (monocot) and soybeans (dicot), for example, can be rotated in various crop rotation cycles in various geographies. Cotton is also a dicot.
“Volunteer” plants are unwanted plants from the prior growing season that emerge in a field planted with crops for the current growing season. Volunteers are basically weeds, and can, like weeds, reduce harvest and yield of the crop of interest for the current growing season. The volunteers divert fertilizer resources and the like from the desired crops.
Unlike plain weeds, volunteers are often specifically engineered to be resistant to some herbicides. Thus, controlling volunteers can be more difficult than controlling naturally occurring weeds.
AAD (aryloxy alkanoate dioxygenase) genes as described herein impart high levels of tolerance to 2,4-D herbicides in plants that are transformed with an AAD gene.
AAD-1 genes (encoding SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, for example, of the attached sequence listing; see WO 2005 107437) also impart high levels of tolerance to phenoxy- and aryloxyphenoxyproplonate herbicides (“fops” such as fluazifop and haloxyfop) to corn and other monocot species transformed with the gene. (Fops are primarily used to control monocots, as dicots tend to have some natural resistance to fops.) Thus, AAD-1 allows the use of some fops as either selection agents or as herbicides on crops where crop destruction would be expected without the AAD-1 gene.
AAD-12 and AAD-13 genes also impart high levels of tolerance to pyridyloxyacetate herbicides (such as triclopyr and fluroxypyr; “pyrs”). Thus, AAD-12 and AAD-13 each allow the use of pyrs as either selection agents or as herbicides on crops where crop destruction would be expected without the AAD-12 or AAD-13 gene.
There are very numerous types of monocot- or grass-only herbicides (that kill monocots).
ACCase inhibitor herbicides include fops and dims.
BRIEF SUMMARY
The subject invention relates in part to the control of AAD-1 monocot volunteers in fields planted with dicot crops such as soybeans or cotton.
The subject invention also relates in part to the recognition that one potential downside to the use of AAD genes is that volunteers can be resistant to 2,4-D, as well as to fops (in the case of AAD-1). In the case of AAD-1, fop herbicides will no longer be effective for control of volunteer AAD-1 corn in fields planted with dicot crops such as soybean or cotton. The subject invention relates in part to the recognition that when an AAD gene is stacked with other herbicide resistance traits (such as glyphosate, glufosinate, and the like) in corn, control of volunteer corn plants in the following year can be an issue.
According to some embodiments of the subject invention, cyclohexanedione (‘dim’) herbicides (such as clethodim, sethoxydim, and the like) are selected, from almost innumerable other options, as being an effective tool for controlling AAD-1 ‘volunteers,’ as AAD genes do not impart tolerance to this class of graminicide chemistry. Dims tend to be moncot-specific.
In addition, the following imidazolinone-class herbicides can be used, in some preferred embodiments, according to the subject invention: imazethapyr, imazamox, and imazaquin. Soy is naturally tolerant to these herbicides, so these imidazolinones can be used for selective control of conventional or herbicide-tolerant varieties of volunteer corn. (Non-commercialized CLEARFIELD cotton and imidazolinone-resistant soy was developed by BASF; cotton is not naturally tolerant to imidazolinones.)
This is assuming that the corn from the previous season was susceptible to imidazolinones. (Soybeans are naturally tolerant to certain imidazolinones.) Corn is generally susceptible to imidazolinones except for CLEARFIELD corn, which is tolerant to imidazolinones.
Use of other types of herbicides, including ALS—(acetolactate synthase) and/or AHAS—(acetohyroxyacid synthase) inhibitors could also be used according to the subject invention for the control of volunteer AAD monocot plants as well.
A third option, according to the subject invention, is to use other herbicides, as described herein, for controlling AAD-1 volunteers. These options include herbicides that provide a relatively fast “burn down,” and non-selective herbicides, such as paraquat, used pre-plant.
AAD-1 corn comprising Event DAS-40278-9 is used in some particularly preferred embodiments.
BRIEF DESCRIPTION OF THE SEQUENCES
SEQ ID NO:1 is the native protein sequence encoded by the AAD-1 v1 gene from Sphingobium herbicidivorans. 
SEQ ID NO:2 is the amino acid sequence encoded by the AAD-1 v2 gene, which removed an internal NotI restriction site.
SEQ ID NO:3 is the amino acid sequence encoded by the AAD-1 v3 gene, which has plant codon usage for expression in a plant.
SEQ ID NO:4 is a sequence for corn Event DAS-40278-9, which includes 5′ and 3′ corn genomic flanking sequences, and the AAD-1 cassette insert sequence.


DETAILED DESCRIPTION
As used herein and unless otherwise specified, preferred dicots are soybeans or cotton.
ACCase inhibitor herbicides include fops and dims.
One aspect of the subject invention includes the use of ‘dim’ herbicides to remove volunteer AAD-1 corn in fields of dicots. In some specific embodiments, the AAD-1 gene is present in the corn as the AAD-1 corn event designated DAS-40278-9 having seed deposited with American Type Culture Collection (ATCC) with Accession No. PTA-10244 (Yellow Dent maize hybrid seed (Zea Mays L.):DAS-40278-9; Deposited in accordance with the Budapest Treaty on behalf of Dow AgroSciences LLC; Date of receipt of seeds/strain(s) by the ATTC: Jul. 10, 2009; viability confirmed Aug. 17, 2009), and progeny derived thereof. See also SEQ ID NO:4. This event is disclosed in U.S. Ser. No. 61/235,248 (filed Aug. 19, 2009). Such AAD-1 monocot volunteers could be present in the following year's soybeans, cotton, or other broadleaf crop. Thus, the subject invention includes the application of a dim (or other as disclosed herein) herbicide to volunteer AAD-1 corn plants, particularly where the corn plant comprises the 40278-9 event.
Because of the specific detoxifying aspects of AAD genes, ‘dim’ herbicides would not be subject to detoxification by AAD-1, and AAD-1 monocot volunteers would remain highly susceptible to dim herbicides. Thus, the subject invention includes the use of one or more dim herbicides, where the AAD-1 gene was used alone or stacked with other herbicide tolerance traits.
In some other preferred embodiments, imidazolinones can be used according to the subject invention to control monocot/corn volunteers in dicot fields. If the corn was susceptible to an imidazolinone and the dicots are resistant to imidazolinones, then a “dim” and/or imidazolinone herbicide could be used in the dicot fields to control the AAD-1 corn volunteers. However, if CLEARFIELD corn (which is resistant to imidazolinones), for example, was planted in the previous season, this would tend to preclude the use of imidazolinones the following season to control corn volunteers in dicot fields.
As alluded to above, selection of herbicides to use on the current planted fields, according to the subject invention, depends in part on the herbicide-tolerant trait(s) that are present in both the volunteers (the monocot crop of the prior season), if any, and the herbicide resistance trait(s) that are present in the field of dicot crops of the current growing season. Thus, additional herbicide chemistries can be selected to provide control of AAD-1 corn depending on the stack, if any, with other herbicide tolerant traits in the corn, and on the tolerance or susceptibility of the dicot (soybeans or cotton) in the field to be treated.
For example, if the volunteer AAD-1 corn was also stacked with a glyphosate- or glufosinate-trait, then glufosinate or glyphosate, respectively, could be used in the soybean field of the current season, assuming that the soybeans also have a resistance trait against glufosinate or glyphosate, respectively.
If the volunteer AAD-1 corn was also stacked with a glyphosate- and glufosinate-traits, then dims and/or imidazolinones could still be used in the soybean fields to control the AAD-1 corn volunteers (again assuming that the corn does not have a resistance trait against an imidazolinone if an imidazolinone is used to control the volunteers).
When an AAD-1 gene was used alone in corn in the previous growing season, imidazolinone, “dims,” glyphosate, and/or glufosinate could be used the following season to control the volunteer AAD-1 corn in planted fields of soybean or cotton that, assuming that the dicots are tolerant/resistant to any one or more of these further herbicides (such as glyphosate and/or glufosinate). That is, glyphosate and/or glufosinate could be used if the soybeans or cotton are engineered or bred to be tolerant to glyphosate and/or glufosinate, respectively.
When an AAD-1 gene was stacked in corn with, for example, a glyphosate or glufosinate resistant trait (such as is found in Roundup Ready [or GAT or other glyphosate-tolerant crops] or Liberty Link [or other glufosinate-tolerant] corn), then imidazolinone and/or a “dim” herbicide could be used to control volunteer AAD-1 corn in the dicot crops (assuming the dicot crop is resistant to imidazolinone if an imidazolinone herbicide is to be used).
Glufosinate or glyphosate, respectively, could also be used, assuming that the dicot crops are resistant to that herbicide and the corn is susceptible. That is, if the soybeans have a trait for glyphosate tolerance but not glufosinate tolerance, for example, then glyphosate could also be used (assuming ROUNDUP READY corn is not the volunteers). If the soybeans have a glufosinate tolerance trait (such as PAT) but not a glyphosate tolerance trait, then glufosinate could also be used (assuming that LIBERTY LINK corn is not the volunteers).
In any of those stacking scenarios (and even if AAD-1, glyphosate tolerance, and glufosinate tolerance, and imidazolinone tolerance traits were all present in the corn of the previous season), dims could be used in the soybean fields to control the AAD-1 corn volunteers.
In some embodiments, AAD-1 monocot volunteers can be controlled, using combinations of herbicides disclosed herein—see Examples 4 and 5, in fields of monocots. These embodiments include control of AAD-1 Event DAS-40278-9 corn volunteers in fields of monocots. Such embodiments include turf-in-turf (AAD-1 turf volunteers in a field of other (non-AAD1) turf.
EXAMPLES
Example 1
Control of Volunteer AAD Maize in a Field Planted with Soybean Using Alternative Herbicides
In one embodiment, volunteer transgenic maize lines containing an AAD expression cassette (AAD-1) are controlled within a field of soybean by the application of a herbicide or combination of herbicides. The specific herbicide used to control the volunteer transgenic AAD maize line is dependent upon the type of soybean seed being planted within the field (e.g., conventional soybean, Round-up Ready Soybean, Liberty Link Soybean, etc.).
Furthermore, the AAD trait may be stacked with other additional herbicide tolerant trait(s) via conventional breeding or a molecular stack. In such an example, the specific herbicide used to control the volunteer AAD maize stacked with another herbicide tolerant trait(s) will be dependent upon the additional herbicide tolerant trait(s) and the type of soybean being planted within the field.
The application of a given herbicide can be made before planting at pre-emergence/burndown or post-emergence after planting to control the volunteer transgenic AAD maize lines. Table 1 lists the herbicides to be used at the different stages of planting (pre-emergence or post-emergence) to control volunteer transgenic AAD maize. At or about a 1× Field Rate concentration of herbicide would be applied, as either a tank mix or alone, to the field for both pre-emergent and post-emergent volunteer control.
The control of transgenic maize plants containing the AAD expression cassette within a field of soybean would be applicable for the control of volunteer AAD transgenic monocot plants (including, but not limited too; corn, rice, sugar cane, switch grass, turf grass species, sorghum, barley, wheat, and oats, and durum) within a field being planted with a dicot crop (including, but not limited too; soybean, cotton, canola, flax, sunflower, legumes, alfalfa, peanut, and tomato). The example described above, in which volunteer transgenic AAD maize plants are controlled in a soybean field, is illustrative of the invention and not intended to restrict the scope of this embodiment.
Example 2
Control of Volunteer Corn (Conventional or Containing Non-AAD Herbicide Tolerance Traits) in a Field Planted with AAD Soybean Using Alternative Herbicides
In an embodiment, volunteer transgenic corn lines (containing the Clear Field trait, Roundup Ready or other Glyphosate Tolerant Trait, Liberty Link Trait, Imidazolonine tolerant trait, or any stacked combination thereof) or volunteer conventional corn lines are controlled within a field of transgenic AAD soybean (AAD-12) by the application of a herbicide or combination of herbicides. The specific herbicide used to control the volunteer corn plants is dependent upon the type of corn seed being planted within the field (e.g., conventional soybean, Round-up Ready Soybean, Liberty Link Soybean, etc.). Additionally, the specific herbicide used to control the volunteer conventional or transgenic corn line is dependent upon the type of AAD transgenic soybean seed (i.e. stacked traits or alone) being planted within the field and the trait possessed by the volunteer corn line.
The application of a given herbicide can be made before planting at pre-emergence/burndown or post-emergence after planting to control the volunteer conventional or transgenic soybean lines. Table 2 and Table 3 list the herbicides to be used at the different stages of planting (pre-emergence or post-emergence) to control volunteer conventional or transgenic corn. At or about a 1× Field Rate concentration of herbicide would be applied, as either a tank mix or alone, to the field for both pre-emergent and post-emergent volunteer control.
The control of conventional or transgenic corn plants containing a herbicide tolerant expression cassette within a field of AAD transgenic soybean (either stacked with other herbicide tolerant traits or alone) would be applicable for the control of a conventional or herbicide tolerant transgenic monocot plant (including, but not limited too; corn, rice, sugar cane, switch grass, turf grass species, sorghum, barley, wheat, and oats, and durum) within a field being planted with an AAD transgenic dicot crop (including, but not limited too; soybean, cotton, canola, flax, sunflower, legumes, alfalfa, peanut, and tomato). The example described above, in which volunteer conventional or transgenic herbicide tolerant corn plants are controlled in a field planted with AAD transgenic soybean, is illustrative of the invention and not intended to restrict the scope of this embodiment.
Example 3
Control of Volunteer AAD-1 Maize in a Field Planted with AAD-12 Cotton Using Alternative Herbicides
In one embodiment, volunteer transgenic maize lines containing an AAD-1 expression cassette are controlled within a field of AAD-12 cotton by the application of a herbicide or combination of herbicides. The specific herbicide used to control the volunteer transgenic AAD-1 maize line is dependent upon the type of AAD-12 cotton seed being planted within the field (e.g., AAD-12 cotton or AAD-12 cotton stacked with Round-up Ready, Liberty Link, or other herbicide tolerant traits).
Furthermore, the AAD-1 trait may be stacked with other additional herbicide tolerant trait(s) via conventional breeding or a molecular stack. In such an example, the specific herbicide used to control the volunteer AAD-1 maize stacked with another herbicide tolerant trait(s) will be dependent upon the additional herbicide tolerant trait(s) and the type of cotton being planted within the field.
The application of a given herbicide can be made before planting at pre-emergence/burndown or post-emergence after planting to control the volunteer transgenic AAD-1 maize lines. Table 4 lists the herbicides to be used at the different stages of planting (pre-emergence or post-emergence) to control volunteer transgenic AAD-1 maize. At or about a 1× Field Rate concentration of herbicide would be applied, as either a tank mix or alone, to the field for both pre-emergent and post-emergent volunteer control.
The control of transgenic maize plants containing the AAD-1 expression cassette within a field of AAD-12 cotton would be applicable for the control of volunteer AAD-1 transgenic monocot plants (including, but not limited too; corn, rice, sugar cane, switch grass, turf grass species, sorghum, barley, wheat, and oats, and durum) within a field being planted with an AAD-12 dicot crop (including, but not limited too; soybean, cotton, canola, flax, sunflower, legumes, alfalfa, peanut, and tomato). The example described above, in which volunteer transgenic AAD-1 maize plants are controlled in an AAD-12 cotton field, is illustrative of the invention and not intended to restrict the scope of this embodiment.







TABLE 1







Control of volunteer AAD-1 (trait alone or stacked with other HT traits)


corn (or other monocot) in soybean (or other dicot crops)











Current year




Previous year
Soybean variety
Herbicides option -


Corn hybrid
being planted
Burndown/PRE
Herbicides option - POST










Application: alone and tank mixes










AAD-1
Soybean, conventional
Glyphosate, Dims, IMI,
Dims, IMI



RR Soybean
glufosinate, paraquat,
Dims, Glyphosate, IMI



LL Soybean
other ALS herbicides not
Dims, Glufosinate, IMI



GAT + ALS
recommended for corn
Dims, IMI, ALS inhibitors not




(need to be aware of
selective for in-corn




planting restrictions)
application



AAD-12
enabled by “ALS gene”
Dims, IMI



AAD-12 + GT

Dims, Glyphosate, IMI



AAD-12 + PAT

Dims, Glufosinate, IMI



AAD-12 + AHAS

Dims, IMI



AAD-12 + PAT + GT

Dims, Glyphosate,





glufosinate, IMI



AAD-12 + PAT + AHAS

Dims, glufosinate, IMI



AAD-12 + GAT

Dims, IMI, glyphosate, ALS





inhibitors not selective for in-





corn application



AAD-12 + PAT + GAT

Dims, IMI, glufosinate





glyphosate, ALS inhibitors





not selective for in-corn





application


AAD-1 + PAT
Soybean, conventional
Glyphosate, Dims, IMI,
Dims, IMI



RR Soybean
paraquat,
Dims, Glyphosate, IMI



LL Soybean
other ALS herbicides not
Dims, IMI



GAT
recommended for corn
Dims, IMI, ALS inhibitors not




(need to be aware of
selective for in-corn




planting restrictions)
application



AAD-12
enabled by “ALS gene”
Dims, IMI



AAD-12 + GT

Dims, Glyphosate, IMI



AAD-12 + PAT

Dims, IMI



AAD-12 + AHAS

Dims, IMI



AAD-12 + PAT + GT

Dims, Glyphosate, IMI



AAD-12 + PAT + AHAS

Dims, IMI



AAD-12 + GAT

Dims, IMI, glyphosate, ALS





inhibitors not selective for in-





corn application



AAD-12 + PAT + GAT

Dims, IMI, glyphosate, ALS





inhibitors not selective for in-





corn application


AAD-1 + GT
Soybean, conventional
Dims, IMI, glufosinate,
Dims, IMI



RR Soybean
paraquat,
Dims, IMI



LL Soybean
other ALS herbicides not
Dims, Glufosinate IMI



GAT
recommended for corn
Dims, IMI, ALS inhibitors not




(need to be aware of
selective for in-corn




planting restrictions)
application



AAD-12
enabled by “ALS gene”
Dims, IMI



AAD-12 + GT

Dims, IMI



AAD-12 + PAT

Dims, Glufosinate, IMI



AAD-12 + AHAS

Dims, IMI



AAD-12 + PAT + GT

Dims, glufosinate, IMI



AAD-12 + PAT + AHAS

Dims, glufosinate, IMI



AAD-12 + GAT

Dims, IMI, ALS inhibitors not





selective for in-corn





application



AAD-12 + PAT + GAT

Dims, IMI, glufosinate, ALS





inhibitors not selective for in-





corn application


AAD-1 + AHAS
Soybean, conventional
Glyphosate, Dims,
Dims



RR Soybean
glufosinate, paraquat,
Dims, Glyphosate



LL Soybean
other ALS herbicides not
Dims, Glufosinate



GAT
recommended for corn
Dims, ALS inhibitors not




(need to be aware of
selective for in-corn




planting restrictions)
application



AAD-12
enabled by “ALS gene”
Dims



AAD-12 + GT

Dims, Glyphosate



AAD-12 + PAT

Dims, Glufosinate



AAD-12 + AHAS

Dims



AAD-12 + PAT + GT

Dims, Glyphosate, glufosinate



AAD-12 + PAT + AHAS

Dims, glufosinate



AAD-12 + GAT

Dims, glyphosate, ALS





inhibitors not selective for in-





corn application



AAD-12 + PAT + GAT

Dims, glufosinate glyphosate,





ALS inhibitors not selective





for in-corn application


AAD-1 + PAT + GT
Soybean, conventional
Dims, IMI, paraquat,
Dims, IMI



RR Soybean
other ALS herbicides not
Dims, IMI



LL Soybean
recommended for corn
Dims, Glufosinate, IMI



GAT
(need to be aware of
Dims, IMI, ALS inhibitors not




planting restrictions)
selective for in-corn




enabled by “ALS gene”
application



AAD-12

Dims, IMI



AAD-12 + GT

Dims, IMI



AAD-12 + PAT

Dims, IMI



AAD-12 + AHAS

Dims, IMI



AAD-12 + PAT + GT

Dims, IMI



AAD-12 + PAT + AHAS

Dims, IMI



AAD-12 + GAT

Dims, IMI, ALS inhibitors not





selective for in-corn





application



AAD-12 + PAT + GAT

Dims, IMI, ALS inhibitors not





selective for in-corn





application


AAD-
Soybean, conventional
Glyphosate, Dims,
Dims


1 + PAT + AHAS
RR Soybean
paraquat,
Dims, Glyphosate



LL Soybean
other ALS herbicides not
Dims



GAT
recommended for corn
Dims, glyphosate, ALS




(need to be aware of
inhibitors not selective for in-




planting restrictions)
corn application



AAD-12
enabled by “ALS gene”
Dims



AAD-12 + GT

Dims, Glyphosate



AAD-12 + PAT

Dims



AAD-12 + AHAS

Dims



AAD-12 + PAT + GT

Dims, Glyphosate



AAD-12 + PAT + AHAS

Dims



AAD-12 + GAT

Dims



AAD-12 + PAT + GAT

Dims, glyphosate, ALS





inhibitors not selective for in-





corn application


AAD-1 + GAT + ALS
Soybean, conventional
Dims, glufosinate,
Dims



RR Soybean
paraquat,
Dims



LL Soybean
other ALS herbicides not
Dims, Glufosinate



GAT + ALS
recommended for corn
Dims



AAD-12
(need to be aware of
Dims



AAD-12 + GT
planting restrictions)
Dims



AAD-12 + PAT
enabled by “ALS gene”
Dims, Glufosinate



AAD-12 + AHAS

Dims



AAD-12 + PAT + GT

Dims, Glufosinate



AAD-12 + PAT + AHAS

Dims, Glufosinate



AAD-12 + GAT

Dims



AAD-12 + PAT + GAT

Dims, Glufosinate


AAD-1 + PAT +
Soybean, conventional
Dims, paraquat,
Dims


GAT + ALS
RR Soybean
other ALS herbicides not
Dims



LL Soybean
recommended for corn
Dims



GAT + ALS
(need to be aware of
Dims



AAD-12
planting restrictions)
Dims



AAD-12 + GT
enabled by “ALS gene”
Dims



AAD-12 + PAT

Dims



AAD-12 + AHAS

Dims



AAD-12 + PAT + GT

Dims



AAD-12 + PAT + AHAS

Dims



AAD-12 + GAT

Dims



AAD-12 + PAT + GAT

Dims





Gene and trait footnotes:


GT = any glyphosate specific tolerance trait including Roundup Ready (CP4), TIPS EPSPS (GA21, Glytol, DMMG), Athenix's EPSPS, GAT only without ALS, GOX, glyphosate decarboxylase, etc.


ALS = double mutant ALS gene insensitive to all ALS herbicide chemistries including IMI herbicides.


AAD-1 = Aryloxyalkanoate dioxygenase gene providing tolerance to all commercial phenoxy auxin and all aryloxyphenoxypropionate (fop) herbicides.


AAD-12 = Aryloxyalkanoate dioxygenase gene providing tolerance to phenoxyacetic auxin and pyridyloxyacetic auxin herbicides.


PAT = phosphinothricin acetyltransferase gene providing tolerance to glutamine synthetase inhibitors including, but not limited to, glufosinate. Similar phenotype is provided by genes such as BAR, DSM1, DSM2, et al.


AHAS = imidazolonine specific tolerance gene associated with point mutation at S623 of ALS gene (maize sequence) or equivalent amino acid in other spp (e.g., S653 in Arabidopsis).


RR = Roundup Ready trait, implies utility of CP4 gene as commercially deployed either alone or in combination with other genes but imparting glyphosate only tolerance.


CL = Clearfield crops, tolerant by nontransgenic means. Primary tolerance is to imidazolinone class of ALS-inhibiting chemistry with some partial tolerance to specific other herbicides with this mode of action. Use of CL designation is intended to distinguish from transgenic use of the AHAS gene.


LL = Liberty Link trait, implies utility of either PAT or BAR gene as commercially deployed either alone or in combination with other genes but imparting only tolerance to glutamine synthetase inhibitors such as glufosinate.


STS = designates resistance to sulfonylurea herbicide chemistry with use of ALS1 gene.


Herbicide footnotes:


IMI = any imidazolinone herbicide including, but not limited to, imazapyr, imazethapyr, imazamox, imazaquin.


DIMS = cyclohexanedione class of herbicides (dims) including, but not limited to, sethoxydim, clethodim, and for the purposes of this demonstration poinoxaden.


Fops = aryloxyphenoxypropionate herbicides (fops) including, but not limited to, quizalofop, haloxyfop, fenoxaprop, fluazifop, et al., their stereospecific isomers or racemic mixtures, and esters, acid, or salts thereof.


ALS inhibitors = any ALS inhibitor to the exclusion of IMI's for the sake of this demonstration (i.e., sulfonylureas, triazolopyrimidine sulfonanalides, sulfonylaminocarbonyltriazolinone).


HPPD = p-Hydroxyphenyl pyruvate dioxygenase inhibitor class of chemistry including but not limited to mesotrione, sulcotrione, isoxaflutole, and pyrazolynate.


MSMA and DSMA = herbicides from the organoarsenicals chemistry family.


N/A = No suitable options available postemergence.













TABLE 2







Control of volunteer corn (or other monocot) [trait alone or stacked with


other HT traits] in AAD-1 Soybean or other dicot crops)











Current year




Previous year
Soybean variety (or


Corn hybrid (or
other dicot) being
Herbicides option - 


other monocot)
planted
Burndown/PRE
Herbicides option - POST










Appl: alone and tank mixes










Conventional corn
AAD-1
Glufosinate, Glyphosate,
2,4-D, Dichloprop, Fops,




Fops, Dims, IMI, Paraquat,
MCPA, MCPP (Mecoprop),




2,4-D, Dichlorprop, MCPA,
Dims, IMI



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, Glyphosate, IMI



AAD-1 + PAT
other ALS herbicides not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




(need to be aware of
Dims, Glufosinate, IMI



AAD-1 + AHAS
planting restrictions)
2,4-D, Dichloprop, Fops,




enabled by “ALS gene”
MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate, IMI



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate, IMI



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application


CL Corn
AAD-1
Glufosinate, Glyphosate,
2,4-D, Dichloprop, Fops,




Fops, Dims, Paraquat, 2,4-
MCPA, MCPP (Mecoprop),




D, Dichlorprop, MCPA,
Dims



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, Glyphosate,



AAD-1 + PAT
other ALS herbicides not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




(need to be aware of
Dims, Glufosinate,



AAD-1 + AHAS
planting restrictions)
2,4-D, Dichloprop, Fops,




enabled by “ALS gene”
MCPA, MCPP (Mecoprop),





Dims,



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate,



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate,



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glyphosate, ALS





inhibitors not selective for in-





corn application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate, ALS inhibitors





not selective for in-corn





application


RR or GT Corn
AAD-1
Glufosinate, Fops, Dims,
2,4-D, Dichloprop, Fops,




IMI, Paraquat, 2,4-D,
MCPA, MCPP (Mecoprop),




Dichlorprop, MCPA,
Dims, IMI



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, IMI



AAD-1 + PAT
other ALS herbicides not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




(need to be aware of
Dims, Glufosinate, IMI



AAD-1 + AHAS
planting restrictions)
2,4-D, Dichloprop, Fops,




enabled by “ALS gene”
MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate, IMI



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate, IMI



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, IMI, ALS inhibitors not





selective for in-corn





application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glufosinate, IMI, ALS





inhibitors not selective for in-





corn application


LL Corn
AAD-1
, Glyphosate, Fops, Dims,
2,4-D, Dichloprop, Fops,




IMI, Paraquat, 2,4-D,
MCPA, MCPP (Mecoprop),




Dichlorprop, MCPA,
Dims, IMI



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, Glyphosate, IMI



AAD-1 + PAT
other ALS herbicides not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




(need to be aware of
Dims,, IMI



AAD-1 + AHAS
planting restrictions)
2,4-D, Dichloprop, Fops,




enabled by “ALS gene”
MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims,, Glyphosate, IMI



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims,, IMI



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims,, Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application


GAT + ALS
AAD-1
Glufosinate, Fops, Dims,
2,4-D, Dichloprop, Fops,




Paraquat, 2,4-D,
MCPA, MCPP (Mecoprop),




Dichlorprop, MCPA,
Dims



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims,,



AAD-1 + PAT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate



AAD-1 + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glufosinate


CL (or AHAS) +
AAD-1
Glyphosate, Fops, Dims,,
2,4-D, Dichloprop, Fops,


PAT

Paraquat, 2,4-D,
MCPA, MCPP (Mecoprop),




Dichlorprop, MCPA,
Dims



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, Glyphosate



AAD-1 + PAT
other ALS herbicides not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




(need to be aware of
Dims,



AAD-1 + AHAS
planting restrictions)
2,4-D, Dichloprop, Fops,




enabled by “ALS gene”
MCPA, MCPP (Mecoprop),





Dims



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glyphosate



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glyphosate, ALS





inhibitors not selective for in-





corn application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glyphosate, ALS





inhibitors not selective for in-





corn application


CL (or AHAS) +
AAD-1
Glufosinate, Fops, Dims,,
2,4-D, Dichloprop, Fops,


GT

Paraquat, 2,4-D,
MCPA, MCPP (Mecoprop),




Dichlorprop, MCPA,
Dims



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, Glufosinate



AAD-1 + PAT
other ALS herbicides not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




(need to be aware of
Dims,



AAD-1 + AHAS
planting restrictions)
2,4-D, Dichloprop, Fops,




enabled by “ALS gene”
MCPA, MCPP (Mecoprop),





Dims



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate, ALS





inhibitors not selective for in-





corn application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glufosinate, ALS





inhibitors not selective for in-





corn application


GT + PAT
AAD-1
Fops, Dims, IMI, Paraquat,
2,4-D, Dichloprop, Fops,




2,4-D, Dichlorprop, MCPA,
MCPA, MCPP (Mecoprop),




MCPP (Mecoprop),
Dims, IMI



AAD-1 + GT
Dicamba, HPPD not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




other ALS herbicides not
Dims, IMI



AAD-1 + PAT
recommended for corn
2,4-D, Dichloprop, Fops,




(need to be aware of
MCPA, MCPP (Mecoprop),




planting restrictions)
Dims, IMI



AAD-1 + AHAS
enabled by “ALS gene”
2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, IMI, ALS inhibitors not





selective for in-corn





application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, IMI, ALS inhibitors not





selective for in-corn





application


GT + CL (or
AAD-1
Fops, Dims, Paraquat, 2,4-
2,4-D, Dichloprop, Fops,


AHAS) + PAT

D, Dichlorprop, MCPA,
MCPA, MCPP (Mecoprop),




MCPP (Mecoprop),
Dims



AAD-1 + GT
Dicamba, HPPD not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




other ALS herbicides not
Dims



AAD-1 + PAT
recommended for corn
2,4-D, Dichloprop, Fops,




(need to be aware of
MCPA, MCPP (Mecoprop),




planting restrictions)
Dims



AAD-1 + AHAS
enabled by “ALS gene”
2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, ALS inhibitors not





selective for in-corn





application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, ALS inhibitors not





selective for in-corn





application


GAT + ALS + PAT
AAD-1
Fops, Dims, Paraquat, 2,4-
2,4-D, Dichloprop, Fops,




D, Dichlorprop, MCPA,
MCPA, MCPP (Mecoprop),




MCPP (Mecoprop),
Dims,



AAD-1 + GT
Dicamba, HPPD not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),





Dims,



AAD-1 + PAT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims,



AAD-1 + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims,



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims,



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims,



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims,



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims,


Dicamba
AAD-1
Glufosinate, Glyphosate,
2,4-D, Dichloprop, Fops,




Fops, Dims, IMI, Paraquat,
MCPA, MCPP (Mecoprop),




2,4-D, Dichlorprop, MCPA,
Dims, IMI



AAD-1 + GT
MCPP (Mecoprop)
2,4-D, Dichloprop, Fops,




other ALS herbicides not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, Glyphosate, IMI



AAD-1 + PAT
(need to be aware of
2,4-D, Dichloprop, Fops,




planting restrictions)
MCPA, MCPP (Mecoprop),




enabled by “ALS gene”
Dims, Glufosinate, IMI



AAD-1 + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate, IMI



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate, IMI



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application


HPPD
AAD-1
Glufosinate, Glyphosate,
2,4-D, Dichloprop, Fops,




Fops, Dims, IMI, Paraquat,
MCPA, MCPP (Mecoprop),




2,4-D, Dichlorprop, MCPA,
Dims, IMI



AAD-1 + GT
MCPP (Mecoprop),
2,4-D, Dichloprop, Fops,




Dicamba, HPPD not
MCPA, MCPP (Mecoprop),




recommended for corn
Dims, Glyphosate, IMI



AAD-1 + PAT
other ALS herbicides not
2,4-D, Dichloprop, Fops,




recommended for corn
MCPA, MCPP (Mecoprop),




(need to be aware of
Dims, Glufosinate, IMI



AAD-1 + AHAS
planting restrictions)
2,4-D, Dichloprop, Fops,




enabled by “ALS gene”
MCPA, MCPP (Mecoprop),





Dims, IMI



AAD-1 + PAT + GT

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate, IMI



AAD-1 + PAT + AHAS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glufosinate, IMI



AAD-1 + GAT + ALS

2,4-D, Dichloprop, Fops,





MCPA, MCPP (Mecoprop),





Dims, Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application



AAD-1 + PAT + GAT +

2,4-D, Dichloprop, Fops,



ALS

MCPA, MCPP (Mecoprop),





Dims, Glufosinate,





Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application





Gene and trait footnotes:


GT = any glyphosate specific tolerance trait including Roundup Ready (CP4), TIPS EPSPS (GA21, Glytol, DMMG), Athenix's EPSPS, GAT only without ALS, GOX, glyphosate decarboxylase, etc.


ALS = double mutant ALS gene insensitive to all ALS herbicide chemistries including IMI herbicides.


AAD-1 = Aryloxyalkanoate dioxygenase gene providing tolerance to all commercial phenoxy auxin and all aryloxyphenoxypropionate (fop) herbicides.


AAD-12 = Aryloxyalkanoate dioxygenase gene providing tolerance to phenoxyacetic auxin and pyridyloxyacetic auxin herbicides.


PAT = phosphinothricin acetyltransferase gene providing tolerance to glutamine synthetase inhibitors including, but not limited to, glufosinate. Similar phenotype is provided by genes such as BAR, DSM1, DSM2, et al.


AHAS = imidazolonine specific tolerance gene associated with point mutation at S623 of ALS gene (maize sequence) or equivalent amino acid in other spp (e.g., S653 in Arabidopsis).


RR = Roundup Ready trait, implies utility of CP4 gene as commercially deployed either alone or in combination with other genes but imparting glyphosate only tolerance.


CL = Clearfield crops, tolerant by nontransgenic means. Primary tolerance is to imidazolinone class of ALS-inhibiting chemistry with some partial tolerance to specific other herbicides with this mode of action. Use of CL designation is intended to distinguish from transgenic use of the AHAS gene.


LL = Liberty Link trait, implies utility of either PAT or BAR gene as commercially deployed either alone or in combination with other genes but imparting only tolerance to glutamine synthetase inhibitors such as glufosinate.


STS = designates resistance to sulfonylurea herbicide chemistry with use of ALS1 gene.


Herbicide footnotes:


IMI = any imidazolinone herbicide including, but not limited to, imazapyr, imazethapyr, imazamox, imazaquin.


DIMS = cyclohexanedione class of herbicides (dims) including, but not limited to, sethoxydim, clethodim, and for the purposes of this demonstration poinoxaden.


Fops = aryloxyphenoxypropionate herbicides (fops) including, but not limited to, quizalofop, haloxyfop, fenoxaprop, fluazifop, et al., their stereospecific isomers or racemic mixtures, and esters, acid, or salts thereof.


ALS inhibitors = any ALS inhibitor to the exclusion of IMI's for the sake of this demonstration (i.e., sulfonylureas, triazolopyrimidine sulfonanalides, sulfonylaminocarbonyltriazolinone).


HPPD = p-Hydroxyphenyl pyruvate dioxygenase inhibitor class of chemistry including but not limited to mesotrione, sulcotrione, isoxaflutole, and pyrazolynate.


MSMA and DSMA = herbicides from the organoarsenicals chemistry family.


N/A = No suitable options available postemergence.













TABLE 3







Control of volunteer corn (or other monocot) [trait alone or stacked with


other HT traits] in AAD-12 Soybean (or other dicot crops)











Current year




Previous year
Soybean variety (or


Corn hybrid (or
other dicot) being
Herbicides option -


other monocot)
planted
Burndown/PRE
Herbicides option - POST










Appl: alone and tank mixes










Conventional corn
AAD-12
Glufosinate, Glyphosate,
2,4-D, Dims, IMI



AAD-12 + GT
Fops, Dims, IMI, Paraquat,
2,4-D, Dims, Glyphosate, IMI



AAD-12 + PAT
2,4-D, Dichlorprop, MCPA,
2,4-D, Dims, Glufosinate, IMI



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims, IMI



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims, Glufosinate,




recommended for corn
Glyphosate, IMI



AAD-12 + PAT + AHAS
other ALS herbicides not
2,4-D, Dims, Glufosinate, IMI



AAD-12 + GAT + ALS
recommended for corn
2,4-D, Dims, Glyphosate, IMI,




(need to be aware of
ALS inhibitors not selective




planting restrictions)
for in-corn application



AAD-12 + PAT + GAT +
enabled by “ALS gene”
2,4-D, Dims, Glufosinate,



ALS

Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application


CL Corn
AAD-12
Glufosinate, Glyphosate,
2,4-D, Dims



AAD-12 + GT
Fops, Dims, Paraquat, 2,4-
2,4-D, Dims, Glyphosate,



AAD-12 + PAT
D, Dichlorprop, MCPA,
2,4-D, Dims, Glufosinate,



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims,



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims, Glufosinate,




recommended for corn
Glyphosate,



AAD-12 + PAT + AHAS
other ALS herbicides not
2,4-D, Dims, Glufosinate,



AAD-12 + GAT + ALS
recommended for corn
2,4-D, Dims, Glyphosate,




(need to be aware of
ALS inhibitors not selective




planting restrictions)
for in-corn application



AAD-12 + PAT + GAT +
enabled by “ALS gene”
2,4-D, Dims, Glufosinate,



ALS

Glyphosate, ALS inhibitors





not selective for in-corn





application


RR or GT Corn
AAD-12
Glufosinate, Fops, Dims,
2,4-D, Dims, IMI



AAD-12 + GT
IMI, Paraquat, 2,4-D,
2,4-D, Dims, IMI



AAD-12 + PAT
Dichlorprop, MCPA,
2,4-D, Dims, Glufosinate, IMI



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims, IMI



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims, Glufosinate, IMI



AAD-12 + PAT + AHAS
recommended for corn
2,4-D, Dims, Glufosinate, IMI



AAD-12 + GAT + ALS
other ALS herbicides not
2,4-D, Dims, IMI, ALS




recommended for corn
inhibitors not selective for in-




(need to be aware of
corn application



AAD-12 + PAT + GAT +
planting restrictions)
2,4-D, Dims, Glufosinate,



ALS
enabled by “ALS gene”
IMI, ALS inhibitors not





selective for in-corn





application


LL Corn
AAD-12
, Glyphosate, Fops, Dims,
2,4-D, Dims, IMI



AAD-12 + GT
IMI, Paraquat, 2,4-D,
2,4-D, Dims, Glyphosate, IMI



AAD-12 + PAT
Dichlorprop, MCPA,
2,4-D, Dims,, IMI



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims, IMI



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims,, Glyphosate, IMI



AAD-12 + PAT + AHAS
recommended for corn
2,4-D, Dims,, IMI



AAD-12 + GAT + ALS
other ALS herbicides not
2,4-D, Dims, Glyphosate, IMI,




recommended for corn
ALS inhibitors not selective




(need to be aware of
for in-corn application



AAD-12 + PAT + GAT +
planting restrictions)
2,4-D, Dims,, Glyphosate,



ALS
enabled by “ALS gene”
IMI, ALS inhibitors not





selective for in-corn





application


GAT + ALS
AAD-12
Glufosinate, Fops, Dims,
2,4-D, Dims



AAD-12 + GT
Paraquat, 2,4-D,
2,4-D, Dims,,



AAD-12 + PAT
Dichlorprop, MCPA,
2,4-D, Dims, Glufosinate



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims, Glufosinate



AAD-12 + PAT + AHAS
recommended for corn
2,4-D, Dims, Glufosinate



AAD-12 + GAT + ALS

2,4-D, Dims



AAD-12 + PAT + FGAT +

2,4-D, Dims, Glufosinate



ALS


CL (or AHAS) +
AAD-12
Glyphosate, Fops, Dims,,
2,4-D, Dims


PAT
AAD-12 + GT
Paraquat, 2,4-D,
2,4-D, Dims, Glyphosate



AAD-12 + PAT
Dichlorprop, MCPA,
2,4-D, Dims,



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims, Glyphosate



AAD-12 + PAT + AHAS
recommended for corn
2,4-D, Dims



AAD-12 + GAT + ALS
other ALS herbicides not
2,4-D, Dims, Glyphosate,




recommended for corn
ALS inhibitors not selective




(need to be aware of
for in-corn application



AAD-12 + PAT + GAT +
planting restrictions)
2,4-D, Dims, Glyphosate,



ALS
enabled by “ALS gene”
ALS inhibitors not selective





for in-corn application


CL (or AHAS) +
AAD-12
Glufosinate, Fops, Dims,,
2,4-D, Dims


GT
AAD-12 + GT
Paraquat, 2,4-D,
2,4-D, Dims, Glufosinate



AAD-12 + PAT
Dichlorprop, MCPA,
2,4-D, Dims,



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims, Glufosinate



AAD-12 + PAT + AHAS
recommended for corn
2,4-D, Dims



AAD-12 + GAT + ALS
other ALS herbicides not
2,4-D, Dims, Glufosinate,




recommended for corn
ALS inhibitors not selective




(need to be aware of
for in-corn application



AAD-12 + PAT + GAT +
planting restrictions)
2,4-D, Dims, Glufosinate,



ALS
enabled by “ALS gene”
ALS inhibitors not selective





for in-corn application


GT + PAT
AAD-12
Fops, Dims, IMI, Paraquat,
2,4-D, Dims, IMI



AAD-12 + GT
2,4-D, Dichlorprop, MCPA,
2,4-D, Dims, IMI



AAD-12 + PAT
MCPP (Mecoprop),
2,4-D, Dims, IMI



AAD-12 + AHAS
Dicamba, HPPD not
2,4-D, Dims, IMI



AAD-12 + PAT + GT
recommended for corn
2,4-D, Dims, IMI



AAD-12 + PAT + AHAS
other ALS herbicides not
2,4-D, Dims, IMI



AAD-12 + GAT + ALS
recommended for corn
2,4-D, Dims, IMI, ALS




(need to be aware of
inhibitors not selective for in-




planting restrictions)
corn application



AAD-12 + PAT + GAT +
enabled by “ALS gene”
2,4-D, Dims, IMI, ALS



ALS

inhibitors not selective for in-





corn application


GT + CL (or
AAD-12
Fops, Dims, Paraquat, 2,4-
2,4-D, Dims


AHAS) + PAT
AAD-12 + GT
D, Dichlorprop, MCPA,
2,4-D, Dims



AAD-12 + PAT
MCPP (Mecoprop),
2,4-D, Dims



AAD-12 + AHAS
Dicamba, HPPD not
2,4-D, Dims



AAD-12 + PAT + GT
recommended for corn
2,4-D, Dims



AAD-12 + PAT + AHAS
other ALS herbicides not
2,4-D, Dims



AAD-12 + GAT + ALS
recommended for corn
2,4-D, Dims, ALS inhibitors




(need to be aware of
not selective for in-corn




planting restrictions)
application



AAD-12 + PAT + GAT +
enabled by “ALS gene”
2,4-D, Dims, ALS inhibitors



ALS

not selective for in-corn





application


GAT + ALS + PAT
AAD-12
Fops, Dims, Paraquat, 2,4-
2,4-D, Dims,



AAD-12 + GT
D, Dichlorprop, MCPA,
2,4-D, Dims,



AAD-12 + PAT
MCPP (Mecoprop),
2,4-D, Dims,



AAD-12 + AHAS
Dicamba, HPPD not
2,4-D, Dims,



AAD-12 + PAT + GT
recommended for corn
2,4-D, Dims,



AAD-12 + PAT + AHAS

2,4-D, Dims,



AAD-12 + GAT + ALS

2,4-D, Dims,



AAD-12 + PAT + GAT +

2,4-D, Dims,



ALS


Dicamba
AAD-12
Glufosinate, Glyphosate,
2,4-D, Dims, IMI



AAD-12 + GT
Fops, Dims, IMI, Paraquat,
2,4-D, Dims, Glyphosate, IMI



AAD-12 + PAT
2,4-D, Dichlorprop, MCPA,
2,4-D, Dims, Glufosinate, IMI



AAD-12 + AHAS
MCPP (Mecoprop)
2,4-D, Dims, IMI



AAD-12 + PAT + GT
other ALS herbicides not
2,4-D, Dims, Glufosinate,




recommended for corn
Glyphosate, IMI



AAD-12 + PAT + AHAS
(need to be aware of
2,4-D, Dims, Glufosinate, IMI



AAD-12 + GAT + ALS
planting restrictions)
2,4-D, Dims, Glyphosate, IMI,




enabled by “ALS gene”
ALS inhibitors not selective





for in-corn application



AAD-12 + PAT + GAT +

2,4-D, Dims, Glufosinate,



ALS

Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application


HPPD
AAD-12
Glufosinate, Glyphosate,
2,4-D, Dims, IMI



AAD-12 + GT
Fops, Dims, IMI, Paraquat,
2,4-D, Dims, Glyphosate, IMI



AAD-12 + PAT
2,4-D, Dichlorprop, MCPA,
2,4-D, Dims, Glufosinate, IMI



AAD-12 + AHAS
MCPP (Mecoprop),
2,4-D, Dims, IMI



AAD-12 + PAT + GT
Dicamba, HPPD not
2,4-D, Dims, Glufosinate,




recommended for corn
Glyphosate, IMI



AAD-12 + PAT + AHAS
other ALS herbicides not
2,4-D, Dims, Glufosinate, IMI



AAD-12 + GAT + ALS
recommended for corn
2,4-D, Dims, Glyphosate, IMI,




(need to be aware of
ALS inhibitors not selective




planting restrictions)
for in-corn application



AAD-12 + PAT + GAT +
enabled by “ALS gene”
2,4-D, Dims, Glufosinate,



ALS

Glyphosate, IMI, ALS





inhibitors not selective for in-





corn application





Gene and trait footnotes:


GT = any glyphosate specific tolerance trait including Roundup Ready (CP4), TIPS EPSPS (GA21, Glytol, DMMG), Athenix's EPSPS, GAT only without ALS, GOX, glyphosate decarboxylase, etc.


ALS = double mutant ALS gene insensitive to all ALS herbicide chemistries including IMI herbicides.


AAD-1 = Aryloxyalkanoate dioxygenase gene providing tolerance to all commercial phenoxy auxin and all aryloxyphenoxypropionate (fop) herbicides.


AAD-12 = Aryloxyalkanoate dioxygenase gene providing tolerance to phenoxyacetic auxin and pyridyloxyacetic auxin herbicides.


PAT = phosphinothricin acetyltransferase gene providing tolerance to glutamine synthetase inhibitors including, but not limited to, glufosinate. Similar phenotype is provided by genes such as BAR, DSM1, DSM2, et al.


AHAS = imidazolonine specific tolerance gene associated with point mutation at S623 of ALS gene (maize sequence) or equivalent amino acid in other spp (e.g., S653 in Arabidopsis).


RR = Roundup Ready trait, implies utility of CP4 gene as commercially deployed either alone or in combination with other genes but imparting glyphosate only tolerance.


CL = Clearfield crops, tolerant by nontransgenic means. Primary tolerance is to imidazolinone class of ALS-inhibiting chemistry with some partial tolerance to specific other herbicides with this mode of action. Use of CL designation is intended to distinguish from transgenic use of the AHAS gene.


LL = Liberty Link trait, implies utility of either PAT or BAR gene as commercially deployed either alone or in combination with other genes but imparting only tolerance to glutamine synthetase inhibitors such as glufosinate.


STS = designates resistance to sulfonylurea herbicide chemistry with use of ALS1 gene.


Herbicide footnotes:


IMI = any imidazolinone herbicide including, but not limited to, imazapyr, imazethapyr, imazamox, imazaquin.


DIMS = cyclohexanedione class of herbicides (dims) including, but not limited to, sethoxydim, clethodim, and for the purposes of this demonstration poinoxaden.


Fops = aryloxyphenoxypropionate herbicides (fops) including, but not limited to, quizalofop, haloxyfop, fenoxaprop, fluazifop, et al., their stereospecific isomers or racemic mixtures, and esters, acid, or salts thereof.


ALS inhibitors = any ALS inhibitor to the exclusion of IMI's for the sake of this demonstration (i.e., sulfonylureas, triazolopyrimidine sulfonanalides, sulfonylaminocarbonyltriazolinone).


HPPD = p-Hydroxyphenyl pyruvate dioxygenase inhibitor class of chemistry including but not limited to mesotrione, sulcotrione, isoxaflutole, and pyrazolynate.


MSMA and DSMA = herbicides from the organoarsenicals chemistry family.


N/A = No suitable options available postemergence.













TABLE 4







Control of volunteer AAD-1 corn (or other monocot crop) (alone or


stacked with other HT traits) in AAD-12-based Cotton (or other dicot crop)











Previous
Current year





year Corn
Cotton hybrid
Herbicides option -
Herbicides option -
Herbicides option -


hybrid
being planted
Burndown/PRE/PPI
POST DIRECTED
POST BROADCAST










Appl: alone and tank mixes











AAD-1
Cotton, CL
Glyphosate, Dims,
Prometryn, Lactofen,
Pyrithiobac, Dims,



Cotton, STS
Tribenuron,
Fluometuron, DSMA,
Imazapyr + Imazethapyr,



Cotton
Trifloxysulfuron,
MSMA, Trifloxysulfuron,
imazamox, imazaquin




Paraquat,
Oxyfluorfen, Paraquat,




Glufosinate, MSMA,
Dimethipin, Diuron,




Flumioxazin,
Linuron, Fomesafen,




Pendimethalin,
Flumioxazin, Dims,




Trifluralin, Prometryn,
Imazapyr + Imazethapyr,




Clomazone,
imazamox, imazaquin



RR Cotton
Fluometuron, Diuron,
Dims, Glyphosate,
Dims, Glyphosate,




Fomesafen,
Prometryn, Lactofen,
Pyrithiobac




Pyrithiobac
Fluometuron, DSMA,





MSMA, Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



LL Cotton

Dims,
Dims,





Glufosinate, Prometryn,
Glufosinate, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



GAT

Dims, Glyphosate, ALS
Dims, Glyphosate,





herbicides
ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Dims,
Dims, Glyphosate,





Glyphosate, Prometryn,
Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + PAT

Dims,
Dims, Glufosinate,





Glufosinate, Prometryn,
Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,
Sulfonylureas,





DSMA, MSMA,





Sulfonylureas,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Glyphosate,
Dims, Glyphosate,



12 + PAT + TIPS

glufosinate, Prometryn,
glufosinate,





Lactofen, Fluometuron,
Pyrithiobac





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-

Dims,
Dims, glufosinate,



12 + PAT + AHAS

glufosinate, Prometryn,
Sulfonylureas,





Lactofen, Fluometuron,
Pyrithiobac





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + GAT

Dims, Glyphosate, ALS
Dims, Glyphosate,





herbicides
ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin


AAD-1 + PAT
CL Cotton
Glyphosate, Dims,
Dims,
Pyrithiobac, Dims,




Tribenuron,
Imazapyr + Imazethapyr,
Imazapyr + Imazethapyr,




Trifloxysulfuron,
imazamox, imazaquin,
imazamox, imazaquin




Paraquat, MSMA,
Prometryn, Lactofen,




Flumioxazin,
Fluometuron, DSMA,




Pendimethalin,
MSMA, Trifloxysulfuron,




Trifluralin, Prometryn,
Oxyfluorfen, Paraquat,




Clomazone,
Dimethipin, Diuron,




Fluometuron, Diuron,
Linuron, Fomesafen,




Fomesafen,
Flumioxazin,



RR Cotton
Pyrithiobac
Dims,
Dims, Glyphosate,





Glyphosate, Prometryn,
Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



LL Cotton

Dims, Prometryn,
Dims,, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



GAT

Dims, Glyphosate, ALS
Dims, Glyphosate,





herbicides
ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones)
triazolinones),






Pyrithiobac



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Glyphosate, Dims,
Dims,





Prometryn, Lactofen,
Glyphosate, Pyrithiobac





Fluometuron, DSMA,





MSMA, Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-12 + PAT

Dims, Prometryn,
Dims,, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,
Sulfonylureas,





DSMA, MSMA,





Sulfonylureas,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-

Dims, Glyphosate,
Dims, Glyphosate,,



12 + PAT + TIPS

Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-

Dims, Prometryn,
Dims,, Sulfonylureas,



12 + PAT + AHAS

Lactofen, Fluometuron,
Pyrithiobac





DSMA, MSMA,





Sulfonylureas,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-12 + GAT

Dims, Glyphosate, ALS
Dims, Glyphosate,





herbicides
ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin


AAD-1 + TIPS
CL Cotton
Dims, Tribenuron,
Prometryn, Lactofen,
Pyrithiobac, Dims,




Trifloxysulfuron,
Fluometuron, DSMA,
Imazapyr + Imazethapyr,




Paraquat,
MSMA, Trifloxysulfuron,
imazamox, imazaquin




Glufosinate, MSMA,
Oxyfluorfen, Paraquat,




Flumioxazin,
Dimethipin, Diuron,




Pendimethalin,
Linuron, Fomesafen,




Trifluralin, Prometryn,
Flumioxazin, Dims,




Clomazone,
Imazapyr + Imazethapyr,




Fluometuron, Diuron,
imazamox, imazaquin



RR Cotton
Fomesafen,
Dims, Glyphosate,
Dims, Pyrithiobac




Pyrithiobac
Prometryn, Lactofen,





Fluometuron, DSMA,





MSMA, Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



LL Cotton

Dims,
Dims,





Glufosinate, Prometryn,
Glufosinate, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



GAT

Dims, ALS herbicides
Dims, ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + PAT

Dims,
Dims,





Glufosinate, Prometryn,
Glufosinate, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,
Sulfonylureas,





DSMA, MSMA,





Sulfonylureas,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, glufosinate,
Dims, glufosinate,



12 + PAT + TIPS

Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Glufosinate,
Dims, glufosinate,



12 + PAT + AHAS

Prometryn, Lactofen,
Sulfonylureas,





Fluometuron, DSMA,
Pyrithiobac





MSMA, Sulfonylureas,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + GAT

Dims, ALS herbicides
Dims, ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin


AAD-
CL Cotton
Glyphosate, Dims,
Dims,
Pyrithiobac, Dims,


1 + AHAS

Tribenuron,
Imazapyr + Imazethapyr,
Imazapyr + Imazethapyr,




Trifloxysulfuron,
imazamox, imazaquin,
imazamox, imazaquin




Paraquat,
Prometryn, Lactofen,




Glufosinate, MSMA,
Fluometuron, DSMA,




Flumioxazin,
MSMA, Oxyfluorfen,




Pendimethalin,
Paraquat, Dimethipin,




Trifluralin, Prometryn,
Diuron, Linuron,




Clomazone,
Fomesafen, Flumioxazin,



RR Cotton
Fluometuron, Diuron,
Dims,
Dims, Glyphosate,




Fomesafen,
Glyphosate, Prometryn,
Pyrithiobac




Pyrithiobac
Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



LL Cotton

Dims, Prometryn,
Dims,





Lactofen, Fluometuron,
Glufosinate, Pyrithiobac





Glufosinate, DSMA,





MSMA, Oxyfluorfen,





Paraquat Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



GAT

Dims, ALS herbicides
Dims, Glyphosate,





(imidazolinones,
ALS herbicides





triazolopyrimidine
(imidazolinones,





sulfonanalides,
triazolopyrimidine





sulfobylamino carbonyl
sulfonanalides,





triazolinones),
sulfobylamino carbonyl





Prometryn, Lactofen,
triazolinones),





Fluometuron,
Pyrithiobac





Glufosinate, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Glyphosate, Dims,
Dims,





Prometryn, Lactofen,
Glyphosate, Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



AAD-12 + PAT

Dims, Prometryn,
Dims,





Lactofen, Fluometuron,
Glufosinate, Pyrithiobac





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-

Dims, Glyphosate,
Dims, Glyphosate,



12 + PAT + TIPS

Prometryn, Lactofen,
glufosinate,





Fluometuron, DSMA,
Pyrithiobac





MSMA, Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-

Dims, Glufosinate,
Dims, glufosinate,



12 + PAT + AHAS

Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



AAD-12 + GAT

Dims, Glyphosate, ALS
Dims, Glyphosate,





herbicides
ALS herbicides





(imidazolinones,
(imidazolinones,





triazolopyrimidine
triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin


AAD-
CL Cotton
Dims, Tribenuron,
Prometryn, Lactofen,
Pyrithiobac, Dims,


1 + PAT + TIPS

Trifloxysulfuron,
Fluometuron, DSMA,
Imazapyr + Imazethapyr,




Paraquat, MSMA,
MSMA, Trifloxysulfuron,
imazamox, imazaquin




Flumioxazin,
Oxyfluorfen, Paraquat,




Pendimethalin,
Dimethipin, Diuron,




Trifluralin, Prometryn,
Linuron, Fomesafen,




Clomazone,
Flumioxazin, Dims,




Fluometuron, Diuron,
Imazapyr + Imazethapyr,




Fomesafen,
imazamox, imazaquin



RR Cotton
Pyrithiobac
Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



LL Cotton

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



GAT

Dims, ALS herbicides
Dims, ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + PAT

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,
Sulfonylureas,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Prometryn,
Dims, Pyrithiobac



12 + PAT + TIPS

Lactofen, Fluometuron,





DSMA, MSMA,





Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Prometryn,
Dims, Sulfonylureas,



12 + PAT + AHAS

Lactofen, Fluometuron,
Pyrithiobac





DSMA, MSMA,





Sulfonylureas,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + GAT

Dims, ALS herbicides
Dims, ALS herbicides





(imidazolinones,
(imidazolinones,





sulfonylureas, triazolopyrimidine
sulfonylureas, triazolopyrimidine





sulfonanalides,
sulfonanalides,





sulfobylamino carbonyl
sulfobylamino carbonyl





triazolinones),
triazolinones),





Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin


AAD-
CL Cotton
Glyphosate, Dims,
Prometryn, Lactofen,
Pyrithiobac, Dims,


1 + PAT + AHAS

Tribenuron,
Fluometuron, DSMA,
Imazapyr + Imazethapyr,




Trifloxysulfuron,
MSMA, Oxyfluorfen,
imazamox, imazaquin




Paraquat, MSMA,
Paraquat, Dimethipin,




Flumioxazin,
Diuron, Linuron,




Pendimethalin,
Fomesafen, Flumioxazin,




Trifluralin, Prometryn,
Dims,




Clomazone,
Imazapyr + Imazethapyr,




Fluometuron, Diuron,
imazamox, imazaquin



RR Cotton
Fomesafen,
Dims, Glyphosate,
Dims, Glyphosate,




Pyrithiobac
Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin,



LL Cotton

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



GAT

Dims, ALS herbicides
Dims, Glyphosate,





(imidazolinones,
ALS herbicides





triazolopyrimidine
(imidazolinones,





sulfonanalides,
triazolopyrimidine





sulfobylamino carbonyl
sulfonanalides,





triazolinones),
sulfobylamino carbonyl





Prometryn, Lactofen,
triazolinones),





Fluometuron, DSMA,
Pyrithiobac





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Dims, Glyphosate,
Dims,





Prometryn, Lactofen,
Glyphosate, Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin,



AAD-12 + PAT

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Glyphosate,
Dims, Glyphosate,



12 + PAT + TIPS

Prometryn, Lactofen,
Pyrithiobac





Fluometuron, DSMA,





MSMA, Trifloxysulfuron,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Prometryn,
Dims, Pyrithiobac



12 + PAT + AHAS

Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + GAT

Dims, ALS herbicides
Dims, Glyphosate,





(imidazolinones,
ALS herbicides





triazolopyrimidine
(imidazolinones,





sulfonanalides,
triazolopyrimidine





sulfobylamino carbonyl
sulfonanalides,





triazolinones),
sulfobylamino carbonyl





Prometryn, Lactofen,
triazolinones),





Fluometuron, DSMA,
Pyrithiobac





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin


AAD-1 + GAT
CL Cotton
Dims, Tribenuron,
Prometryn, Lactofen,
Pyrithiobac, Dims,




Trifloxysulfuron,
Fluometuron, DSMA,
Imazapyr + Imazethapyr,




Paraquat,
MSMA, Oxyfluorfen,
imazamox, imazaquin




Glufosinate, MSMA,
Paraquat, Dimethipin,




Flumioxazin,
Diuron, Linuron,




Pendimethalin,
Fomesafen, Flumioxazin,




Trifluralin, Prometryn,
Dims,



RR Cotton
Clomazone,
Dims, Prometryn,
Dims, Pyrithiobac




Fluometuron, Diuron,
Lactofen, Fluometuron,




Fomesafen,
DSMA, MSMA,




Pyrithiobac
Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



LL Cotton

Dims, Glufosinate,
Dims,





Prometryn, Lactofen,
Glufosinate, Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin,



GAT

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + PAT

Dims, Glufosinate,
Dims,





Prometryn, Lactofen,
Glufosinate, Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin,



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Prometryn,
Dims, glufosinate,



12 + PAT + TIPS

Lactofen, Fluometuron,
Pyrithiobac





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Prometryn,
Dims, glufosinate,



12 + PAT + AHAS

Lactofen, Fluometuron,
Pyrithiobac





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + GAT

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin


AAD-
CL Cotton
Dims, Tribenuron,
Prometryn, Lactofen,
Pyrithiobac, Dims,


1 + PAT + GAT

Trifloxysulfuron,
Fluometuron, DSMA,
Imazapyr + Imazethapyr,




Paraquat,
MSMA, Oxyfluorfen,
imazamox, imazaquin




Glufosinate, MSMA,
Paraquat, Dimethipin,




Flumioxazin,
Diuron, Linuron,




Pendimethalin,
Fomesafen, Flumioxazin,




Trifluralin, Prometryn,
Dims,



RR Cotton
Clomazone,
Dims, Prometryn,
Dims, Pyrithiobac




Fluometuron, Diuron,
Lactofen, Fluometuron,




Fomesafen,
DSMA, MSMA,




Pyrithiobac
Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



LL Cotton

Dims, Glufosinate,
Dims,





Prometryn, Lactofen,
Glufosinate, Pyrithiobac





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin,



GAT

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin



AAD-12

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + TIPS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + PAT

Dims, Glufosinate,
Dims, Pyrithiobac





Prometryn, Lactofen,





Fluometuron, DSMA,





MSMA, Oxyfluorfen,





Paraquat, Dimethipin,





Diuron, Linuron,





Fomesafen, Flumioxazin,



AAD-12 + AHAS

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Prometryn,
Dims, Pyrithiobac



12 + PAT + TIPS

Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-

Dims, Prometryn,
Dims, Pyrithiobac



12 + PAT + AHAS

Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin,



AAD-12 + GAT

Dims, Prometryn,
Dims, Pyrithiobac





Lactofen, Fluometuron,





DSMA, MSMA,





Oxyfluorfen, Paraquat,





Dimethipin, Diuron,





Linuron, Fomesafen,





Flumioxazin





Gene and trait footnotes:


GT = any glyphosate specific tolerance trait including Roundup Ready (CP4), TIPS EPSPS (GA21, Glytol, DMMG), Athenix's EPSPS, GAT only without ALS, GOX, glyphosate decarboxylase, etc.


ALS = double mutant ALS gene insensitive to all ALS herbicide chemistries including IMI herbicides.


AAD-1 = Aryloxyalkanoate dioxygenase gene providing tolerance to all commercial phenoxy auxin and all aryloxyphenoxypropionate (fop) herbicides.


AAD-12 = Aryloxyalkanoate dioxygenase gene providing tolerance to phenoxyacetic auxin and pyridyloxyacetic auxin herbicides.


PAT = phosphinothricin acetyltransferase gene providing tolerance to glutamine synthetase inhibitors including, but not limited to, glufosinate. Similar phenotype is provided by genes such as BAR, DSM1, DSM2, et al.


AHAS = imidazolonine specific tolerance gene associated with point mutation at S623 of ALS gene (maize sequence) or equivalent amino acid in other spp (e.g., S653 in Arabidopsis).


RR = Roundup Ready trait, implies utility of CP4 gene as commercially deployed either alone or in combination with other genes but imparting glyphosate only tolerance.


CL = Clearfield crops, tolerant by nontransgenic means. Primary tolerance is to imidazolinone class of ALS-inhibiting chemistry with some partial tolerance to specific other herbicides with this mode of action. Use of CL designation is intended to distinguish from transgenic use of the AHAS gene.


LL = Liberty Link trait, implies utility of either PAT or BAR gene as commercially deployed either alone or in combination with other genes but imparting only tolerance to glutamine synthetase inhibitors such as glufosinate.


STS = designates resistance to sulfonylurea herbicide chemistry with use of ALS1 gene.


Herbicide footnotes:


IMI = any imidazolinone herbicide including, but not limited to, imazapyr, imazethapyr, imazamox, imazaquin.


DIMS = cyclohexanedione class of herbicides (dims) including, but not limited to, sethoxydim, clethodim, and for the purposes of this demonstration poinoxaden.


Fops = aryloxyphenoxypropionate herbicides (fops) including, but not limited to, quizalofop, haloxyfop, fenoxaprop, fluazifop, et al., their stereospecific isomers or racemic mixtures, and esters, acid, or salts thereof.


ALS inhibitors = any ALS inhibitor to the exclusion of IMI's for the sake of this demonstration (i.e., sulfonylureas, triazolopyrimidine sulfonanalides, sulfonylaminocarbonyltriazolinone).


HPPD = p-Hydroxyphenyl pyruvate dioxygenase inhibitor class of chemistry including but not limited to mesotrione, sulcotrione, isoxaflutole, and pyrazolynate.


MSMA and DSMA = herbicides from the organoarsenicals chemistry family.


N/A = No suitable options available postemergence.






Example 4
Control of Volunteer AAD Maize in a Field Planted with Maize Using Alternative Herbicides
In another embodiment, volunteer transgenic maize lines containing the AAD expression cassette (AAD-1) are controlled within a field of maize by the application of a herbicide or combination of herbicides. The specific herbicide used to control the volunteer transgenic AAD maize line is dependent upon the type of maize seed being planted within the field (e.g., Clear Field Maize, Round-up Ready Maize, Liberty Link Maize, etc.).
Furthermore, the AAD trait may be stacked with other additional herbicide tolerant trait(s) via conventional breeding or a molecular stack. In such an example, the specific herbicide used to control the volunteer AAD maize stacked with another herbicide tolerant trait(s) will be dependent upon the additional herbicide tolerant trait(s) and the type of maize being planted within the field.
The application of a given herbicide can be made before planting at pre-emergence/burndown or post-emergence after planting to control the volunteer transgenic AAD maize lines. Table 5 lists the herbicides to be used at the different stages of planting (pre-emergence or post-emergence) to control volunteer transgenic AAD maize. At or about a 1× Field Rate concentration of herbicide would be applied, as either a tank mix or alone, to the field for both pre-emergent and post-emergent volunteer control.
The control of transgenic maize plants containing the AAD expression cassette within a field of maize would be applicable for the control of an AAD transgenic monocot plant (including, but not limited too; corn, rice, sugar cane, switch grass, turf grass species, sorghum, barley, wheat, and oats, and durum) within a field being planted with a monocot crop (including, but not limited too; corn, rice, sugar cane, switch grass, turf grass species, sorghum, barley, wheat, and oats, and durum). The example described above, in which volunteer transgenic AAD maize plants are controlled in a maize field, is illustrative of the invention and not intended to restrict the scope of this embodiment.







TABLE 5







Control of volunteer AAD-1 (trait alone or stacked with other HT traits)


corn (or other monocot) in corn (or other monocot)










Previous year
2008 Corn hybrid
Herbicides option -



Corn hybrid
being planted
Burndown/PRE
Herbicides option - POST










Application: alone and tank mixes










AAD-1
CL Corn
Glyphosate, Dims,
IMI



RR Corn
glufosinate, paraquat
glyphosate



LL Corn
IMI or other ALS
glufosinate



GAT + ALS
herbicides not
glyphosate, IMI, ALS




recommended for corn
inhibitors not usually




(need to be aware of
recommended for in-corn




planting restrictions)
application



AAD-1 + PAT

glufosinate



AAD-1 + GT

glyphosate



AAD-1 + AHAS

IMI



AAD-1 + PAT + GT

glufosinate, glyphosate



AAD-1 + PAT + AHAS

glufosinate, glyphosate,





IMI



AAD-1 + PAT + GAT

Glyphosate, glufosinate,





IMI, ALS inhibitors not





usually recommended for





in-corn application



AAD-1 + GAT

glyphosate, ALS inhibitors





not usually recommended





for in-corn application


AAD-1 + PAT
CL Corn
Glyphosate, Dims,
IMI



RR Corn
paraquat
glyphosate



LL Corn
IMI or other ALS
N/A



GAT
herbicides not
glyphosate, Imazapyr +




recommended for corn
imazethapyr, imazamox,




(need to be aware of
imazaquin, ALS inhibitors




planting restrictions)
not usually recommended





for in-corn application



AAD-1 Corn

N/A



AAD-1 + GT

glyphosate



AAD-1 + AHAS

IMI



AAD-1 + PAT + GT

glyphosate



AAD-1 + PAT + AHAS

IMI



AAD-1 + PAT + GAT

glufosinate, IMI, ALS





inhibitors not usually





recommended for in-corn





application



AAD-1 + GAT

glyphosate, IMI, ALS





inhibitors not usually





recommended for in-corn





application


AAD-1 + GT
CL Corn
Dims, glufosinate,
IMI



RR Corn
paraquat
N/A



LL Corn
IMI or other ALS
glufosinate



GAT
herbicides not
IMI, ALS inhibitors not




recommended for corn
usually recommended for




(need to be aware of
in-corn application



AAD-1 Corn
planting restrictions)
N/A



AAD-1 + PAT

glufosinate



AAD-1 + AHAS

IMI



AAD-1 + PAT + GT

glufosinate



AAD-1 + PAT + AHAS

Glufosinate, IMI



AAD-1 + GAT

IMI, ALS inhibitors not





usually recommended for





in-corn application



AAD-1 + PAT + GAT

glufosinate, IMI, ALS





inhibitors not usually





recommended for in-corn





application


AAD-1 + AHAS
CL Corn
Glyphosate, Dims,
N/A



RR Corn
glufosinate, paraquat
glyphosate



LL Corn
ALS herbicides not
glufosinate



GAT
recommended for corn
IMI, ALS inhibitors not




(need to be aware of
usually recommended for




planting restrictions)
in-corn application



AAD-1 Corn

N/A



AAD-1 + PAT

glufosinate



AAD-1 + GT

glyphosate



AAD-1 + PAT + GT

glufosinate, glyphosate



AAD-1 + PAT + AHAS

glufosinate



AAD-1 + GAT

Glyphosate, ALS inhibitors





not usually recommended





for in-corn application



AAD-1 + PAT + GAT

Glyphosate, glufosinate,





ALS inhibitors not usually





recommended for in-corn





application


AAD-1 + PAT + GT
CL Corn
Dims, paraquat
IMI



RR Corn
IMI or other ALS
N/A



LL Corn
herbicides not
N/A



GAT
recommended for corn
IMI, ALS inhibitors not




(need to be aware of
usually recommended for




planting restrictions)
in-corn application



AAD-1

N/A



AAD-1 + PAT

N/A



AAD-1 + GT

N/A



AAD-1 + AHAS

IMI



AAD-1 + PAT + AHAS

IMI



AAD-1 + GAT

ALS inhibitors not usually





recommended for in-corn





application



AAD-1 + PAT + GAT

ALS inhibitors not usually





recommended for in-corn





application


AAD-
CL Corn
Glyphosate, Dims,
N/A


1 + PAT + AHAS
RR Corn
paraquat
glyphosate



LL Corn
IMI or other ALS
N/A



GAT
herbicides not
Glyphosate, ALS inhibitors




recommended for corn
not usually recommended




(need to be aware of
for in-corn application



AAD-1
planting restrictions)
N/A



AAD-1 + PAT

N/A



AAD-1 + GT

glyphosate



AAD-1 + AHAS

N/A



AAD-1 + PAT + GT

glyphosate



AAD-1 + GAT

glyphosate, ALS inhibitors





not usually recommended





for in-corn application



AAD-

glyphosate, ALS inhibitors



1 + PAT + GAT + ALS

not usually recommended





for in-corn application


AAD-1 + GAT + ALS
CL Corn
Dims, glufosinate,
N/A



RR Corn
paraquat
N/A



LL Corn
IMI or other ALS
glufosinate



GAT + ALS
herbicides not
N/A



AAD-1
recommended for corn
N/A



AAD-1 + PAT
(need to be aware of
glufosinate



AAD-1 + GT
planting restrictions)
N/A



AAD-1 + AHAS

N/A



AAD-1 + PAT + GT

glufosinate



AAD-1 + PAT + AHAS

glufosinate



AAD-

glufosinate



1 + PAT + GAT + ALS


AAD-1 + PAT +
CL Corn
Dims, paraquat
N/A


GAT + ALS
RR Corn
IMI or other ALS
N/A



LL Corn
herbicides not
N/A



GAT + ALS
recommended for corn
N/A



AAD-1
(need to be aware of
N/A



AAD-1 + PAT
planting restrictions)
N/A



AAD-1 + GT

N/A



AAD-1 + AHAS

N/A



AAD-1 + PAT + GT

N/A



AAD-1 + PAT + AHAS

N/A





Gene and trait footnotes:


GT = any glyphosate specific tolerance trait including Roundup Ready (CP4), TIPS EPSPS (GA21, Glytol, DMMG), Athenix's EPSPS, GAT only without ALS, GOX, glyphosate decarboxylase, etc.


ALS = double mutant ALS gene insensitive to all ALS herbicide chemistries including IMI herbicides.


AAD-1 = Aryloxyalkanoate dioxygenase gene providing tolerance to all commercial phenoxy auxin and all aryloxyphenoxypropionate (fop) herbicides.


AAD-12 = Aryloxyalkanoate dioxygenase gene providing tolerance to phenoxyacetic auxin and pyridyloxyacetic auxin herbicides.


PAT = phosphinothricin acetyltransferase gene providing tolerance to glutamine synthetase inhibitors including, but not limited to, glufosinate. Similar phenotype is provided by genes such as BAR, DSM1, DSM2, et al.


AHAS = imidazolonine specific tolerance gene associated with point mutation at S623 of ALS gene (maize sequence) or equivalent amino acid in other spp (e.g., S653 in Arabidopsis).


RR = Roundup Ready trait, implies utility of CP4 gene as commercially deployed either alone or in combination with other genes but imparting glyphosate only tolerance.


CL = Clearfield crops, tolerant by nontransgenic means. Primary tolerance is to imidazolinone class of ALS-inhibiting chemistry with some partial tolerance to specific other herbicides with this mode of action. Use of CL designation is intended to distinguish from transgenic use of the AHAS gene.


LL = Liberty Link trait, implies utility of either PAT or BAR gene as commercially deployed either alone or in combination with other genes but imparting only tolerance to glutamine synthetase inhibitors such as glufosinate.


STS = designates resistance to sulfonylurea herbicide chemistry with use of ALS1 gene.


Herbicide footnotes:


IMI = any imidazolinone herbicide including, but not limited to, imazapyr, imazethapyr, imazamox, imazaquin.


DIMS = cyclohexanedione class of herbicides (dims) including, but not limited to, sethoxydim, clethodim, and for the purposes of this demonstration poinoxaden.


Fops = aryloxyphenoxypropionate herbicides (fops) including, but not limited to, quizalofop, haloxyfop, fenoxaprop, fluazifop, et al., their stereospecific isomers or racemic mixtures, and esters, acid, or salts thereof.


ALS inhibitors = any ALS inhibitor to the exclusion of IMI's for the sake of this demonstration (i.e., sulfonylureas, triazolopyrimidine sulfonanalides, sulfonylaminocarbonyltriazolinone).


HPPD = p-Hydroxyphenyl pyruvate dioxygenase inhibitor class of chemistry including but not limited to mesotrione, sulcotrione, isoxaflutole, and pyrazolynate.


MSMA and DSMA = herbicides from the organoarsenicals chemistry family.


N/A = No suitable options available postemergence.






Example 5
Control of Volunteer Maize in a Field Planted with AAD-Maize Using Alternative Herbicides
In an embodiment, volunteer transgenic maize lines (containing the Clear Field trait, Roundup Ready or other Glyphosate Tolerant Trait, Liberty Link Trait, Imidazolonine tolerant trait, or any stacked combination thereof) or volunteer conventional maize lines are controlled within a field of transgenic AAD maize (AAD-1) by the application of aryloxyphenoxypropionate herbicide (Fops) including, but not limited to; quizalofop, haloxyfop, fenoxaprop, fluazifop, et al., their stereospecific isomers or racemic mixtures, and esters, acid, or salts thereof. In addition, the Fops herbicide may be applied with another herbicide(s) to control the volunteer maize lines described above.
The specific herbicide used to control the volunteer conventional or transgenic maize line is dependent upon the type of AAD transgenic maize seed (i.e. stacked traits or alone) being planted within the field and the trait possessed by the volunteer maize line. For example an AAD-1 transgenic maize line that has been stacked with another herbicide tolerant trait such as PAT could be sprayed with a herbicide mixture containing a Fop and glufosinate, but only where the preceding volunteer plants do not contain PAT (or other glufosinate tolerant trait) and AAD-1.
The application of a given herbicide can be made before planting at pre-emergence/burndown or post-emergence after planting to control the volunteer conventional or transgenic maize lines. Table 6 lists the herbicides to be used at the different stages of planting (pre-emergence or post-emergence) to control volunteer conventional or transgenic maize. At or about a 1× Field Rate concentration of herbicide would be applied, as either a tank mix or alone, to the field for both pre-emergent and post-emergent volunteer control.
The control of conventional maize plants or transgenic maize plants containing a herbicide tolerant expression cassette within a field of AAD transgenic maize (either stacked with other herbicide tolerant traits or alone) would be applicable for the control of a herbicide tolerant transgenic monocot plant (including, but not limited too; corn, rice, sugar cane, switch grass, turf grass species, sorghum, barley, wheat, and oats, and durum) within a field being planted with an AAD monocot crop (including, but not limited too; corn, rice, sugar cane, switch grass, turf grass species, sorghum, barley, wheat, and oats, and durum). The example described above, in which volunteer transgenic herbicide tolerant maize plants are controlled in a field planted with AAD transgenic maize, is illustrative of the invention and not intended to restrict the scope of this embodiment.







TABLE 6







Control of volunteer corn (or other monocots) in AAD-1 (alone or


stacked with other HT traits) corn (or other monocots)










Previous year
2008 Corn hybrid being
Herbicides option -
Herbicides option -


Corn hybrid
planted
Burndown/PRE
POST










Application: alone and tank mixes










Conventional
AAD-1
Glyphosate, Dims, Fops,
Fops


corn
AAD-1 + PAT
glufosinate, paraquat
Fops, glufosinate



AAD-1 + GT
IMI or other ALS
Fops, glyphosate



AAD-1 + GAT + ALS
herbicides not
Fops, glyphosate, ALS




recommended for corn
inhibitors not selective




(need to be aware of
for in-corn application



AAD-1 + AHAS
planting restrictions)
Fops, glyphosate, IMI



AAD-1 + CL

Fops, IMI



AAD-1 + PAT + GT

Fops, glufosinate,





glyphosate



AAD-1 + PAT + GAT + ALS

Fops, glufosinate,





glyphosate, IMI, ALS





inhibitors not selective





for in-corn application



AAD-1 + PAT + AHAS

Fops, glufosinate,





glyphosate, IMI


CL Corn
AAD-1
Glyphosate, Dims, Fops,
Fops



AAD-1 + PAT
glufosinate, paraquat
Fops, glufosinate



AAD-1 + GT
other ALS herbicides not
Fops, glyphosate



AAD-1 + GAT + ALS
recommended for corn
Fops, glyphosate, ALS




(need to be aware of
inhibitors not selective




planting restrictions)
for in-corn application



AAD-1 + AHAS
Dims, Fops, glufosinate,
Fops, glyphosate



AAD-1 + CL
paraquat
Fops



AAD-1 + PAT + GT
IMI or other ALS
Fops, glufosinate,




herbicides not
glyphosate



AAD-1 + PAT + GAT + ALS
recommended for corn
Fops, glufosinate,




(need to be aware of
glyphosate, ALS




planting restrictions)
inhibitors not selective





for in-corn application



AAD-1 + PAT + AHAS

Fops, glufosinate,





glyphosate


RR or GT Corn
AAD-1

Fops



AAD-1 + PAT

Fops, glufosinate



AAD-1 + GT

Fops



AAD-1 + GAT + ALS

Fops, ALS inhibitors not





selective for in-corn





application



AAD-1 + AHAS

Fops, IMI



AAD-1 + CL

Fops, IMI



AAD-1 + PAT + GT

Fops, glufosinate



AAD-1 + PAT + GAT + ALS

Fops, glufosinate, IMI,





ALS inhibitors not





selective for in-corn





application



AAD-1 + PAT + AHAS

Fops, glufosinate, IMI


LL Corn
AAD-1
Glyphosate, Dims, Fops,
Fops



AAD-1 + PAT
paraquat
Fops



AAD-1 + GT
IMI or other ALS
Fops, glyphosate



AAD-1 + GAT + ALS
herbicides not
Fops, glyphosate, ALS




recommended for corn
inhibitors not selective




(need to be aware of
for in-corn application



AAD-1 + AHAS
planting restrictions)
Fops, glyphosate, IMI



AAD-1 + CL

Fops, IMI



AAD-1 + PAT + GT

Fops, glyphosate



AAD-1 + PAT + GAT + ALS

Fops, glyphosate, IMI,





ALS inhibitors not





selective for in-corn





application



AAD-1 + PAT + AHAS

Fops, glyphosate, IMI


GAT + ALS
AAD-1
Glyphosate, Dims, Fops,
Fops



AAD-1 + PAT
glufosinate, paraquat
Fops, glufosinate



AAD-1 + GT

Fops



AAD-1 + GAT + ALS

Fops, ALS inhibitors not





selective for in-corn





application



AAD-1 + AHAS

Fops



AAD-1 + CL

Fops



AAD-1 + PAT + GT

Fops, glufosinate



AAD-1 + PAT + GAT + ALS

Fops, glufosinate



AAD-1 + PAT + AHAS

Fops, glufosinate


CL (or AHAS) +
AAD-1
Glyphosate, Dims, Fops,
Fops


PAT
AAD-1 + PAT
glufosinate, paraquat
Fops



AAD-1 + GT
other ALS herbicides not
Fops, glyphosate



AAD-1 + GAT + ALS
recommended for corn
Fops, glyphosate, ALS




(need to be aware of
inhibitors not selective




planting restrictions)
for in-corn application



AAD-1 + AHAS

Fops, glyphosate



AAD-1 + CL

Fops, IMI



AAD-1 + PAT + GT

Fops, glyphosate



AAD-1 + PAT + GAT + ALS

Fops, glyphosate, ALS





inhibitors not selective





for in-corn application



AAD-1 + PAT + AHAS

Fops, glyphosate


CL (or AHAS) +
AAD-1
Dims, Fops, glufosinate,
Fops


GT
AAD-1 + PAT
paraquat
Fops, glufosinate



AAD-1 + GT
other ALS herbicides not
Fops



AAD-1 + GAT + ALS
recommended for corn
Fops, ALS inhibitors not




(need to be aware of
selective for in-corn




planting restrictions)
application



AAD-1 + AHAS

Fops



AAD-1 + CL

Fops



AAD-1 + PAT + GT

Fops, glufosinate



AAD-1 + PAT + GAT + ALS

Fops, glufosinate, ALS





inhibitors not selective





for in-corn application



AAD-1 + PAT + AHAS

Fops, glufosinate


GT + PAT
AAD-1
Dims, Fops, paraquat
Fops



AAD-1 + PAT
IMI or other ALS
Fops



AAD-1 + GT
herbicides not
Fops



AAD-1 + GAT + ALS
recommended for corn
Fops, ALS inhibitors not




(need to be aware of
selective for in-corn




planting restrictions)
application



AAD-1 + AHAS

Fops, IMI



AAD-1 + CL

Fops, IMI



AAD-1 + PAT + GT

Fops



AAD-1 + PAT + GAT + ALS

Fops, IMI, ALS





inhibitors not selective





for in-corn application



AAD-1 + PAT + AHAS

Fops, IMI


GT + CL (or
AAD-1
Dims, Fops, paraquat
Fops


AHAS) + PAT
AAD-1 + PAT
other ALS herbicides not
Fops



AAD-1 + GT
recommended for corn
Fops



AAD-1 + GAT + ALS
(need to be aware of
Fops, ALS inhibitors not




planting restrictions)
selective for in-corn





application



AAD-1 + AHAS

Fops



AAD-1 + CL

Fops



AAD-1 + PAT + GT

Fops



AAD-1 + PAT + GAT + ALS

Fops, ALS inhibitors not





selective for in-corn





application



AAD-1 + PAT + AHAS

Fops


GAT + ALS + PAT
AAD-1
Dims, Fops, paraquat
Fops



AAD-1 + PAT

Fops



AAD-1 + GT

Fops



AAD-1 + GAT + ALS

Fops



AAD-1 + AHAS

Fops



AAD-1 + CL

Fops



AAD-1 + PAT + GT

Fops



AAD-1 + PAT + GAT + ALS

Fops



AAD-1 + PAT + AHAS

Fops





Gene and trait footnotes:


GT = any glyphosate specific tolerance trait including Roundup Ready (CP4), TIPS EPSPS (GA21, Glytol, DMMG), Athenix's EPSPS, GAT only without ALS, GOX, glyphosate decarboxylase, etc.


ALS = double mutant ALS gene insensitive to all ALS herbicide chemistries including IMI herbicides.


AAD-1 = Aryloxyalkanoate dioxygenase gene providing tolerance to all commercial phenoxy auxin and all aryloxyphenoxypropionate (fop) herbicides.


AAD-12 = Aryloxyalkanoate dioxygenase gene providing tolerance to phenoxyacetic auxin and pyridyloxyacetic auxin herbicides.


PAT = phosphinothricin acetyltransferase gene providing tolerance to glutamine synthetase inhibitors including, but not limited to, glufosinate. Similar phenotype is provided by genes such as BAR, DSM1, DSM2, et al.


AHAS = imidazolonine specific tolerance gene associated with point mutation at S623 of ALS gene (maize sequence) or equivalent amino acid in other spp (e.g., S653 in Arabidopsis).


RR = Roundup Ready trait, implies utility of CP4 gene as commercially deployed either alone or in combination with other genes but imparting glyphosate only tolerance.


CL = Clearfield crops, tolerant by nontransgenic means. Primary tolerance is to imidazolinone class of ALS-inhibiting chemistry with some partial tolerance to specific other herbicides with this mode of action. Use of CL designation is intended to distinguish from transgenic use of the AHAS gene.


LL = Liberty Link trait, implies utility of either PAT or BAR gene as commercially deployed either alone or in combination with other genes but imparting only tolerance to glutamine synthetase inhibitors such as glufosinate.


STS = designates resistance to sulfonylurea herbicide chemistry with use of ALS1 gene.


Herbicide footnotes:


IMI = any imidazolinone herbicide including, but not limited to, imazapyr, imazethapyr, imazamox, imazaquin.


DIMS = cyclohexanedione class of herbicides (dims) including, but not limited to, sethoxydim, clethodim, and for the purposes of this demonstration poinoxaden.


Fops = aryloxyphenoxypropionate herbicides (fops) including, but not limited to, quizalofop, haloxyfop, fenoxaprop, fluazifop, et al., their stereospecific isomers or racemic mixtures, and esters, acid, or salts thereof.


ALS inhibitors = any ALS inhibitor to the exclusion of IMI's for the sake of this demonstration (i.e., sulfonylureas, triazolopyrimidine sulfonanalides, sulfonylaminocarbonyltriazolinone).


HPPD = p-Hydroxyphenyl pyruvate dioxygenase inhibitor class of chemistry including but not limited to mesotrione, sulcotrione, isoxaflutole, and pyrazolynate.


MSMA and DSMA = herbicides from the organoarsenicals chemistry family.


N/A = No suitable options available postemergence.






Example 5
Testing of Post-Emergent Herbicide Application on Transgenic Maize Containing AAD-1 and PAT
Transgenic Hi-II maize plants containing the AAD-1 and PAT expression cassette (RB7 MAR v3::Zea Maize Ubiquitin Promoter v2::AAD-1 v3::Zea Maize Per5 3′UTR v2::Rice Actin1 Promoter v2::PAT v3::Zea Maize Lipase 3′UTR v1) from pDAB3404 and Hi-II non-transgenic control plants were propagated for testing. Seed were planted into Metro Mix Media (Sun Gro Horticulture Inc., Bellevue, Wash.) and subirrigated with Hoagland's solution until wet, then allowed to gravity drain. Pots were placed in a glasshouse environment set to 27° C. until the germinated plants reached the V1-V3 growth stage. At or about, the V1-V3 growth stage, the maize plants were spayed using a track sprayer and a drop down nozzle calibrated to deliver a187 L/ha solution of herbicide at the 1× Field Rate (Table 7). After treatment the plants were placed in the greenhouse and scored via visual injury assessment after 14 days. Table 7 also lists the tolerance of the plants to the applied herbicides as determined by the visual injury assessment.







TABLE 7







Graminicidal active ingredients on AAD-1 + PAT + glyphosate tol


Tolerance (−/+)











Trt
Chemical Name
WT
3404
Rate (1x)/Comment





Select
clethodim
−
−
26.25-52.5 g ai/ha


Poast
sethoxydim
−
−
105-210 g ai/ha


Achieve Liquid
tralkoxydim
−
−
100 g ai/ha


Pursuit
imazethapyr
−/+
−/+
Need higher than 70 g ai/ha rate (50% injury @






70 g ai/ha)


Raptor
imazamox
−
−
44 g ai/ha


Scepter
imazaquin
−/+
−/+
Need higher than 50 g ai/ha rate (50% injury @






50 g ai/ha)


Assure II
quizalofop
−
+
35-70 g ai/ha


Discover
clodinafop
−
+
28-56 g ai/ha


Hoelon
diclofop-methyl
−
−
Not active on grasses


Fusilade DX
fluazifop-P-butyl
−
+
105 g ai/ha


Gallant Super
haloxyfop-methyl R
−
+
17.5-35 g ai/ha


Puma Super
fenoxaprop-P-ethyl
−
+
23-46 g ai/ha


Clincher SF
cyhalofop-butyl
−
+
280 g ai/ha


Axial
pinoxaden
+/−
+/−
Not active but small amt of control on both WT






and AAD-1


Glyphomax XRT
glyphosate-
−
+
420-840 g ai/ha



isopropylammonium


Liberty
glufosinate-ammonium
−
+
240-480 g ai/ha


Hoelon
diclofop-methyl
−
−
Not active on grasses


Fusilade DX
fluazifop-P-butyl
−
+
105 g ai/ha


Gallant Super
haloxyfop-methyl R
−
+
17.5-35 g ai/ha


Puma Super
fenoxaprop-P-ethyl
−
+
23-46 g ai/ha


Clincher SF
cyhalofop-butyl
−
+
280 g ai/ha


Axial
pinoxaden
+/−
+/−
Not active but small amt of control on both WT






and AAD-1


Glyphomax XRT
glyphosate-
−
+
420-840 g ai/ha



isopropylammonium


Liberty
glufosinate-ammonium
−
+
240-480 g ai/ha





Note:


3404 column contains molecular stack of AAD-1, and PAT crossed conentionally with CP4 for glyphosate tole

## Claims

1. A method of controlling Aryloxy Alkanoate Dioxygenase (AAD-1) volunteer corn plants, comprising Event DAS-40278-9 as available in seed deposited under ATCC deposit number PTA-10244, in a field comprising dicot plants, said volunteer corn plants comprising an AAD-1 gene, wherein said method comprises applying a herbicide to said volunteer corn plants.

2. The method of claim 1, wherein said dicot plants are selected from the group consisting of soybeans and cotton.

3. The method of claim 1, wherein said AAD-1 gene encodes a protein selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3.

4. The method of claim 1, wherein said herbicide is selected from the group consisting of a cyclohexanedione and an imidazolinone.

5. The method of claim 4, wherein said herbicide is a cyclohexanedione selected from the group consisting of clethodim and sethosydim.

6. The method of claim 4, wherein said herbicide is an imidazolinone selected from the group consisting of imazethapyr, imazamox, and imazaquin.

7. The method of claim 6, wherein said dicot plants are soybeans, and said volunteer corn plants are other than imidazolinone-tolerant corn.

8. The method of claim 1, wherein said volunteer corn plants comprise a glyphosate- and/or a glufosinate-tolerance gene.

9. A method of controlling AAD-1 volunteer corn plants in a field comprising dicot plants, said volunteer corn plants comprising SEQ ID NO:4 of corn Event DAS-40278-9, wherein said method comprises applying a herbicide to said volunteer corn plants, wherein said volunteer corn plants are susceptible to said herbicide, and said dicot plants are tolerant to said herbicide.

10. The method of claim 9, wherein said herbicide is selected from the group consisting of glyphosate and glufosinate.

11. The method of claim 9, wherein said dicot plants are selected from the group consisting of soybeans and cotton.

12. The method of claim 9, wherein said herbicide is selected from the group consisting of a cyclohexanedione and an imidazolinone.

13. The method of claim 12, wherein said volunteer corn plants comprise a glyphosate- and/or a glufosinate-tolerance gene.

14. The method of claim 13, wherein said herbicide is selected from the group consisting of a cyclohexanedione and an imidazolinone.

15. The method of claim 9, wherein said herbicide is selected from the group consisting of acetolactate synthase inhibitors and acetohydroxyacid synthase inhibitors.

16. The method of claim 9, wherein said AAD-1 gene encodes a protein selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3.

17. The method of claim 9, wherein said herbicide is a cyclohexanedione selected from the group consisting of clethodim and sethoxydim.

18. The method of claim 9, wherein said herbicide is an imidazolinone selected from the group consisting of imazethapyr, imazamox, and imazaquin.

19. The method of claim 18, wherein said dicot plants are soybeans, and said corn is other than imidazolinone-tolerant corn.

20. The method of claim 1, wherein said herbicide is selected from the group consisting of glyphosate and glufosinate.

21. The method of claim 1, wherein said herbicide is selected from the group consisting of acetolactate synthase inhibitors and acetohydroxyacid synthase inhibitors.