# Patent

## Title
Recombinant butyrylcholinesterases and truncates thereof

## Abstract

Isolated nucleic acids encoding polypeptides that exhibit butyrylcholinesterase (BChE) enzyme activity are disclosed, along with molecular criteria for preparing such nucleic acids, including codon optimization. Methods of preparing modified and/or truncated BChE molecules having selected properties, especially selective formation of monomers, are also described. Vectors and cells containing and/or expressing the nucleic acids are also disclosed.

## Background
This application is a continuation of U.S. application Ser. No. 13/517,081, filed 19 Jun. 2012, now U.S. Pat. No. 8,729,245, which was a National Phase under 35 U.S.C. 371 of International Application PCT/US2010/03225, filed 21 Dec. 2010, which claims priority of U.S. provisional Application 61/284,444, filed 21 Dec. 2009, the disclosures of all of which are herein incorporated by reference in their entirety.


FIELD OF THE INVENTION
The present invention provides methods for the production of recombinant butyrylcholinesterases using polynucleotides codon-optimized for expression in mammalian, especially human, cells, including truncates thereof.
BACKGROUND OF THE INVENTION
The general term cholinesterase (ChE) refers to a family of enzymes involved in nerve impulse transmission. Cholinesterase-inhibiting substances such as organophosphate compounds or carbamate insecticides or drugs prevent the breakdown of acetylcholine, resulting in a buildup of acetylcholine, thereby causing hyperactivity of the nervous system. When humans breathe or are otherwise exposed to these compounds, which has led to the development of these compounds as “nerve gases” or chemical warfare agents.
Those enzymes which preferentially hydrolyze other types of esters such as butyrylcholine, and whose enzymatic activity is sensitive to the chemical inhibitor tetraisopropylpyrophosphoramide (also known as iso-OMPA), are called butyrylcholinesterases (BChE, EC 3.1.1.8). Butyrylcholinesterase (BChE), also known as plasma, serum, benzoyl, false, or Type II ChE, has more than eleven isoenzyme variants and preferentially uses butyrylcholine and benzoylcholine as in vitro substrates. BChE is found in mammalian blood plasma, liver, pancreas, intestinal mucosa, the white matter of the central nervous system, smooth muscle, and heart. BChE is sometimes referred to as serum cholinesterase as opposed to red cell cholinesterase (AChE).
The use of cholinesterases as pre-treatment drugs has been successfully demonstrated in animals, including non-human primates. For example, pretreatment of rhesus monkeys with fetal bovine serum-derived AChE or horse serum-derived BChE protected them against a challenge of two to five times the LD50 of pinacolyl methylphosphonofluoridate (soman), a highly toxic organophophate compound used as a war-gas [Broomfield, et al. J. Pharmacol. Exp. Ther. (1991) 259:633-638; Wolfe, et al. Toxicol Appl Pharmacol (1992) 117(2):189-193]. In addition to preventing lethality, the pretreatment prevented behavioral incapacitation after the soman challenge, as measured by the serial probe recognition task or the equilibrium platform performance task. Administration of sufficient exogenous human BChE can protect mice, rats, and monkeys from multiple lethal-dose organophosphate intoxication [see for example Raveh, et al. Biochemical Pharmacology (1993) 42:2465-2474; Raveh, et al. Toxicol. Appl. Pharmacol. (1997) 145:43-53; Allon, et al. Toxicol. Sci. (1998) 43:121-128]. Purified human BChE has been used to treat organophosphate poisoning in humans, with no significant adverse immunological or psychological effects (Cascio, et al. Minerva Anestesiol (1998) 54:337).
In addition to its efficacy in hydrolyzing organophosphate toxins, there is strong evidence that BChE is the major detoxifying enzyme of cocaine [Xie, et al. Molec. Pharmacol. (1999) 55:83-91]. Cocaine is metabolized by three major routes: hydrolysis by BChE to form ecgonine methyl ester, N-demethylation from norcocaine, and non-enzymatic hydrolysis to form benzoylcholine. Studies have shown a direct correlation between low BChE levels and episodes of life-threatening cocaine toxicity. A recent study has confirmed that a decrease of cocaine half-life in vitro correlated with the addition of purified human BChE.
In view of the significant pharmaceutical potential of ChE enzymes, research has focused on development of recombinant methods to produce them. Recombinant enzymes, as opposed to those derived from plasma, have a much lower risk of transmission of infectious agents, including viruses such as hepatitis C and HIV.
The cDNA sequences have been cloned for both human AChE (see U.S. Pat. No. 5,595,903) and human BChE [see U.S. Pat. No. 5,215,909 to Soreq; Prody, et al. Proc. Natl. Acad. Sci. USA (1987) 84:3555-3559; McTiernan, et al. Proc. Natl. Acad. Sci USA (1987) 84:6682-6686]. The amino acid sequence of wild-type human BChE, as well as of several BChE variants with single amino acid changes, is set forth in U.S. Pat. No. 6,001,625.
Notably, none of the recombinant expression systems reported to date have the ability to produce BChE in quantities sufficient to allow development of the enzyme as a drug to treat such conditions as organophosphate poisoning, post-surgical apnea, or cocaine intoxication. However, an additional problem is longevity. Thus, the longer the BChE remains in the system of a person treated, the longer it is available for detoxification. Such lifespan is referred to as the “mean residence time” (MRT) in the system.
The current state of art for BChE is directed to making the tetramer form because it is the “native form” and is thus considered to be more stable with a longer “mean residence time” (MRT). However, due to the very large size of the tetramer, it is difficult to prepare. In addition, such preparation usually results in a mixture of tetramer, dimer and monomer forms with low yield. Such preparation has proven both very cumbersome and very expensive to purify and characterize. As a result, it is probably too expensive to make as a useful therapeutic product. In view of the foregoing, more powerful methods of producing BChE are needed.
In sum, the current obstacles in the manufacture of the native BChE molecule as a bioscavenger product which are: 1) low yield, 2) complex manufacturing process (milk), 3) short half-life (thus requiring pegylation), 4) highly heterogeneous product (difficult to characterize and obtain FDA approval) and 5) high cost of the product.
The present invention addresses at least some of these problems by providing inter alia a truncated monomeric form of BChE. While the monomer form is just as active as the tetrameric form, it has been considered to be less stable (i.e., have a lower “MRT”) than the tetramer. This may be because the protein made is not properly glycosylated and/or sialylated. Applicants have identified a cell line and clone to accomplish this result. Furthermore, if the full length BChE is made, the cells produce a mixture of monomer, dimer and tetramer so that the present invention also provides a means of producing preferably the monomeric form.
BRIEF SUMMARY OF THE INVENTION
In one aspect, the present invention relates to an isolated nucleic acid, which may be DNA, such as a cDNA, or RNA, that encodes a polypeptide having BChE enzyme activity (as determined, for example, using the well known Ellman assay), wherein the nucleic acid has been codon-optimized, such as where the percentage of guanine plus cytosine (G+C) nucleotides in the coding region of the nucleic acid is greater than about 40%, or is greater than 45%, or is greater than 50%, or is greater than 55%, or is at least 60%, or is greater than 60% but not greater than 80%.
In specific embodiments, the isolated nucleic acid does not contain internal structural elements that reduce expression levels of the subject genetic construct, including an internal TATA-box, an internal ribosomal entry site, or a splice donor or acceptor site.
In one embodiment, the isolated nucleic acid of the invention contains or encodes at least one Kozak sequence, preferably upstream of the start site.
The isolated nucleic acid of the invention also encodes one or more glycosylation and/or sialylation sites on the synthesized polypeptide. In a preferred embodiment, these are sufficient in number to permit full glycosylation and/or sialylation of the encoded BChE.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the codon optimized nucleotide (SEQ ID NO: 1) and corresponding amino acid (SEQ ID NO: 2) sequences of a BChE of the invention. Such sequences contain inserted restriction and other sites, such as the human signal or leader sequence made up of amino acids 1 to 21, where full length BChE begins with the sequence EDD starting at amino acid residue 22.
FIG. 2 shows the nucleotide (SEQ ID NO: 5) and corresponding amino acid (SEQ ID NO: 6) sequences of native human BChE (i.e., without codon optimization), containing a goat casein leader or signal polypeptide (amino acids 1 to 15) for transgenic expression in goat's milk, and where the BChE native polypeptide begins with the sequence EDD starting at amino acid residue 17 (the glutamic acid numbered 1 and inserted arginine as 1′).
FIG. 3 shows the codon-optimized nucleotide (SEQ ID NO: 3) and amino acid sequence (SEQ ID NO: 4) of a BChE truncate of the invention, containing a human signal or leader sequence made up of amino acids 1 to 21, where the BChE truncate begins with the sequence EDD starting at amino acid residue 22.



DEFINITIONS
Unless expressly stated otherwise elsewhere herein, each of the following terms has the stated meaning:
The term “butyrylcholinesterase enzyme” or “BChE enzyme” means a polypeptide capable of hydrolyzing acetylcholine and butyrylcholine, and whose catalytic activity is inhibited by the chemical inhibitor iso-OMPA. Preferred BChE enzymes to be produced by the present invention are mammalian BChE enzymes. The term “BChE enzyme” also encompasses pharmaceutically acceptable salts of such a polypeptide.
The term “recombinant butyrylcholinesterase” or “recombinant BChE” means a BChE enzyme produced by a transiently transfected, stably transfected, or transgenic host cell or animal as directed by one of the expression constructs of the invention as well as by direct chemical synthesis. The term “recombinant BChE” also encompasses pharmaceutically acceptable salts of such a polypeptide.
The term “vector sequences” means any of several nucleic acid sequences established in the art which have utility in the recombinant DNA technologies of the invention to facilitate the cloning and/or propagation of the expression constructs including (but not limited to) plasmids, cosmids, phage vectors, viral vectors, and yeast artificial chromosomes.
The term “expression construct” or “construct” means a nucleic acid sequence comprising a target nucleic acid sequence or sequences whose expression is desired, operably linked to sequence elements which provide for the proper transcription and translation of the target nucleic acid sequence(s) within the chosen host cells. Such sequence elements may include a promoter, a signal sequence for secretion, a polyadenylation signal, intronic sequences, insulator sequences, and other elements described in the invention. The “expression construct” or “construct” may further comprise vector sequences.
The term “operably linked” means that a target nucleic acid sequence and one or more regulatory sequences (e.g., promoter or signal sequences) are physically linked so as to permit expression of the polypeptide encoded by the target nucleic acid sequence within a host cell.
The term “promoter” means a region of DNA involved in binding of RNA polymerase to initiate transcription.
The term “signal sequence” means a nucleic acid sequence which, when incorporated into a nucleic acid sequence encoding a polypeptide, directs secretion of the translated polypeptide (e.g., a BChE enzyme and/or a glycosyltransferase) from cells which express said polypeptide. The signal sequence is preferably located at the 5′-end of the nucleic acid sequence encoding the polypeptide, such that the polypeptide sequence encoded by the signal sequence is located at the N-terminus of the translated polypeptide, and is commonly a leader sequence. The term “signal peptide” means the peptide sequence resulting from translation of a signal sequence.
The term “host cell” means a cell which has been transfected with one or more expression constructs of the invention. Such host cells include mammalian cells in in vitro culture and cells found in vivo in an animal. Preferred in vitro cultured mammalian host cells include Per.C6
The term “transfection” means the process of introducing one or more of the expression constructs of the invention into a host cell by any of the methods well established in the art, including (but not limited to) microinjection, electroporation, liposome-mediated transfection, calcium phosphate-mediated transfection, or virus-mediated transfection. A host cell into which an expression construct of the invention has been introduced by transfection is “transfected”. The term “transiently transfected cell” means a host cell wherein the introduced expression construct is not permanently integrated into the genome of the host cell or its progeny, and therefore may be eliminated from the host cell or its progeny over time. The term “stably transfected cell” means a host cell wherein the introduced expression construct has integrated into the genome of the host cell and its progeny.
In accordance with the present invention, the term “DNA segment” refers to a DNA polymer, in the form of a separate fragment or as a component of a larger DNA construct, which has been derived from DNA isolated at least once in substantially pure form, i.e., free of contaminating endogenous materials and in a quantity or concentration enabling identification, manipulation, and recovery of the segment and its component nucleotide sequences by standard biochemical methods, for example, using a cloning vector. Such segments are provided in the form of an open reading frame uninterrupted by internal non-translated sequences, or introns, which are typically present in eukaryotic genes. Sequences of non-translated DNA may be present downstream from the open reading frame, where the same do not interfere with manipulation or expression of the coding regions.
“Isolated” in the context of the present invention with respect to polypeptides (or polynucleotides) means that the material is removed from its original environment (e.g., the natural environment if it is naturally occurring). For example, a naturally-occurring polynucleotide or polypeptide present in a living organism is not isolated, but the same polynucleotide or polypeptide, separated from some or all of the co-existing materials in the natural system, is isolated. Such polynucleotides could be part of a vector and/or such polynucleotides or polypeptides could be part of a composition, and still be isolated in that such vector or composition is not part of its natural environment. The polypeptides and polynucleotides of the present invention are preferably provided in an isolated form, and preferably are purified to homogeneity.
The term “coding region” refers to that portion of a gene which either naturally or normally codes for the expression product of that gene in its natural genomic environment, i.e., the region coding in vivo for the native expression product of the gene. The coding region can be from a normal, mutated or altered gene, or can even be from a DNA sequence, or gene, wholly synthesized in the laboratory using methods well known to those of skill in the art of DNA synthesis.
In accordance with the present invention, the term “nucleotide sequence” refers to a heteropolymer of deoxyribonucleotides. Generally, DNA segments encoding the proteins provided by this invention are assembled from cDNA fragments and short oligonucleotide linkers, or from a series of oligonucleotides, to provide a synthetic gene which is capable of being expressed in a recombinant transcriptional unit comprising regulatory elements derived from a microbial or viral operon.
The term “expression product” means that polypeptide or protein that is the natural translation product of the gene and any nucleic acid sequence coding equivalents resulting from genetic code degeneracy and thus coding for the same amino acid(s).
As used herein, the terms “portion,” “segment,” “truncate” and “fragment,” when used in relation to polypeptides, refer to a continuous sequence of residues, such as amino acid residues, which sequence forms a subset of a larger sequence. For example, if a polypeptide were subjected to treatment with any of the common endopeptidases, such as trypsin or chymotrypsin, the oligopeptides resulting from such treatment would represent portions, segments or fragments of the starting polypeptide. When used in relation to a polynucleotides, such terms refer to the products produced by treatment of said polynucleotides with any of the common endonucleases.
The term “fragment,” when referring to a coding sequence, means a portion of DNA comprising less than the complete coding region whose expression product retains essentially the same biological function or activity as the expression product of the complete coding region.
The term “open reading frame (ORF)” means a series of triplets coding for amino acids without any termination codons and is a sequence (potentially) translatable into protein.
As used herein, reference to a DNA sequence includes both single stranded and double stranded DNA. Thus, the specific sequence, unless the context indicates otherwise, refers to the single strand DNA of such sequence, the duplex of such sequence with its complement (double stranded DNA) and the complement of such sequence.
In accordance with the present invention, the term “percent identity” or “percent identical,” when referring to a nucleotide or amino acid sequence, means that the sequence is compared to a claimed or described sequence after alignment of the sequence to be compared (the “Compared Sequence”) with the described or claimed sequence (the “Reference Sequence”). The Percent Identity is then determined according to the following formula:

Percent Identity=100[1−(C/R)]

wherein C is the number of differences between the Reference Sequence and the Compared Sequence over the length of alignment between these sequences wherein (i) each base or amino acid in the Reference Sequence that does not have a corresponding aligned base or amino acid in the Compared Sequence and (ii) each gap in the Reference Sequence and (iii) each aligned base or amino acid in the Reference Sequence that is different from an aligned base or amino acid in the Compared Sequence, constitutes a difference; and R is the number of bases or amino acids in the Reference Sequence over the length of the alignment with the Compared Sequence with any gap created in the Reference Sequence also being counted as a base or amino acid.

If an alignment exists between the Compared Sequence and the Reference Sequence for which the percent identity as calculated above is about equal to or greater than a specified minimum Percent Identity then the Compared Sequence has the specified minimum percent identity to the Reference Sequence even though alignments may exist in which the hereinabove calculated Percent Identity is less than the specified Percent Identity.
DETAILED DESCRIPTION OF THE INVENTION
Butyrylcholinesterase derived from human serum is a globular, tetrameric molecule with a molecular mass of approximately 340 kDa. Nine Asn-linked carbohydrate chains are found on each 574-amino acid subunit (which subunit begins with amino acid 17 in SEQ ID NO: 6). The tetrameric form of BChE is stable and has been preferred in the art for therapeutic uses. BChE enzymes produced according to the instant invention have the ability to bind and/or hydrolyze organophosphate, such as pesticides, and war gases, succinylcholine, or cocaine.
The BChE enzyme of the present invention comprises an amino acid sequence that is substantially identical to a sequence found in a mammalian BChE, more preferably, human BChE, and may be produced as a tetramer, a trimer, a dimer, or a monomer. In a preferred embodiment, the synthesized BChE of the invention has a glycosylation and/or sialylation profile that is substantially similar, if not identical, to that of native human BChE.
The BChE produced according to the present invention is preferably in monomeric form with high MRT, thus reducing the need for expensive post-synthetic modification to increase MRT, such as pegylation (i.e., attachment of one or more molecules of polyethylene glycol of varying molecular weight and structure). Conversely, BChE expressed recombinantly in CHO (Chinese hamster ovary) cells was found not to be mostly in the more stable tetrameric form, but rather consisted of approximately 55% dimers, 10-30% tetramers and 15-40% monomers (Blong, et al. Biochem. J., Vol. 327, pp 747-757 (1997)).
Recent studies have shown that a proline-rich amino acid sequence from the N-terminus of the collagen-tail protein caused acetylcholinesterase to assemble into the tetrameric form (Bon, et al. J. Biol. Chem. (1997) 272(5):3016-3021 and Krejci, et al. J. Biol. Chem. (1997) 272:22840-22847). To greatly increase the amount of monomeric BChE enzyme formed according to the invention, the DNA sequence encoding the BChE enzyme of the invention preferably does not comprise a proline-rich attachment domain (PRAD), which otherwise recruits recombinant BChE subunits (e.g., monomers, dimers and trimers) to form tetrameric associations.
The non-tetrameric forms of BChE are also useful in applications which do not require in vivo administration, such as the clean-up of lands used to store organophosphate compounds, as well as decontamination of military equipment exposed to organophosphates. For ex vivo use, these non-tetrameric forms of BChE may be incorporated into sponges, sprays, cleaning solutions or other materials useful for the topical application of the enzyme to equipment and personnel. These forms of the enzyme may also be applied externally to the skin and clothes of human patients who have been exposed to organophosphate compounds. The non-tetrameric forms of the enzyme may also find applications as barriers and sealants applied to the seams and closures of military clothing and gas masks used in chemical warfare situations.
The present invention also provides vectors that include polynucleotides of the present invention, host cells which are genetically engineered with vectors of the invention and the production of polypeptides of the invention by recombinant techniques.
Host cells are genetically engineered (i.e., transduced or transformed or transfected) with the vectors of this invention which may be, for example, a cloning vector or an expression vector, preferably in the form of a plasmid, a viral particle, a phage, etc. The engineered host cells can be cultured in conventional nutrient media modified as required for activating promoters, selecting transformants or amplifying the genes of the present invention. The culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to the ordinarily skilled artisan.
The polynucleotides of the present invention are preferably employed for producing polypeptides by recombinant techniques. Such a polynucleotide may be included in any one of a variety of expression vectors for expressing a polypeptide. Such vectors include chromosomal, nonchromosomal and synthetic DNA sequences, e.g., derivatives of SV40; bacterial plasmids; phage DNA; baculovirus; yeast plasmids; vectors derived from combinations of plasmids and phage DNA, viral DNA such as vaccinia, adenovirus, fowl pox virus, and pseudorabies. However, any other vector may be used as long as it is replicable and viable in the host cell.
The appropriate DNA sequence may be inserted into the vector by a variety of procedures. In general, the DNA sequence is inserted into an appropriate restriction endonuclease site(s) by procedures known in the art. Such procedures and others are deemed to be within the scope of those skilled in the art.
The DNA sequence in the expression vector is operatively linked to an appropriate expression control sequence(s) (such as a promoter and/or enhancer, in either cis or trans location) to direct mRNA synthesis. As representative examples of such promoters, there may be mentioned: LTR or SV40 promoter, the phage lambda PL promoter and other promoters known to control expression of genes in eukaryotic, preferably human, cells. The expression vector also contains a ribosome binding site for translation initiation and a transcription terminator. The vector may also include appropriate sequences for amplifying expression, especially where these are designed to work optimally in human cells, for example, Per.C6 cells.
The vector containing the appropriate DNA sequence as herein described, as well as an appropriate promoter or control sequence, may be employed to transform an appropriate host to permit the host to express the protein.
As representative examples of appropriate hosts, there may be mentioned human cells, preferably Per.C6 cells (available from Percivia, Cambridge, Mass.).
More particularly, the present invention also includes recombinant constructs comprising one or more of the sequences as broadly described above. The constructs comprise a vector, such as a plasmid or viral vector, into which a sequence of the invention has been inserted, in a forward or reverse orientation. In a preferred aspect of this embodiment, the construct further comprises regulatory sequences, including, for example, a promoter, operably linked to the sequence. Large numbers of suitable vectors and promoters are known to those of skill in the art and are commercially available.
Promoter regions can be selected from any desired gene using CAT (chloramphenicol transferase) vectors or other vectors with selectable markers. Two appropriate vectors are pKK232-8 and pCM7. Eukaryotic promoters include CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein-I. Selection of the appropriate vector and promoter is well within the level of ordinary skill in the art.
In a further embodiment, the present invention relates to host cells containing the above-described constructs. The host cell is preferably a human cell, since the nucleic acids of the invention have been optimized for such human expression. Introduction of the construct into the host cell can be effected by calcium phosphate transfection, DEAE-Dextran mediated transfection, or electroporation (Davis, L., Dibner, M., Battey, I., Basic Methods in Molecular Biology, (1986)). The constructs in host cells can be used in a conventional manner to produce the gene product encoded by the recombinant sequence. Alternatively, the polypeptides of the invention can be synthetically produced by conventional peptide synthesizers.
Appropriate cloning and expression vectors for use with eukaryotic hosts are described by Sambrook, et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y., (1989), the disclosure of which is hereby incorporated by reference.
Transcription of the DNA encoding the polypeptides of the present invention by higher eukaryotes, especially human cells, is increased by inserting an enhancer sequence into the vector. Enhancers are cis-acting elements of DNA, usually from about 10 to about 300 bp that act on a promoter to increase its transcription. Examples include the SV40 enhancer on the late side of the replication origin by 100 to 270, a cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and adenovirus enhancers.
Generally, recombinant expression vectors will include origins of replication and selectable markers permitting transformation of the host cell and a promoter derived from a highly-expressed gene to direct transcription of a downstream structural sequence. Such promoters can be derived from operons encoding glycolytic enzymes such as 3-phosphoglycerate kinase (PGK), α-factor, acid phosphatase, or heat shock proteins, among others. The heterologous structural sequence is assembled in appropriate phase with translation initiation and termination sequences, and preferably, a leader sequence capable of directing secretion of translated protein into the periplasmic space or extracellular medium. Optionally, the heterologous sequence can encode a fusion protein including an N-terminal identification peptide imparting desired characteristics, e.g., stabilization or simplified purification of expressed recombinant product.
Following transformation of a suitable host strain and growth of the host strain to an appropriate cell density, the selected promoter is induced by appropriate means (e.g., temperature shift or chemical induction) and cells are cultured for an additional period. Cells are typically harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract retained for further purification.
Mammalian, especially human, cell expression vectors will comprise an origin of replication, a suitable promoter and enhancer, and also any necessary ribosome binding sites, polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5′ flanking non-transcribed sequences. DNA sequences derived from the SV40 splice, and polyadenylation sites may be used to provide the required non-transcribed genetic elements.
The polypeptide can be recovered and purified from recombinant cell cultures by methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography and lectin chromatography. Protein refolding steps can be used, as necessary, in completing configuration of the mature protein. Finally, high performance liquid chromatography (HPLC) can be employed for final purification steps.
Because of the degeneracy of the genetic code, more than one codon may be employed to encode a particular amino acid. However, not all codons encoding the same amino acid are utilized equally. For optimal expression in a cell, e.g. a human cell, the nucleic acid, e.g. DNA, to be expressed may be codon optimized so as to contain a coding region utilizing the codons most commonly employed by that species or that particular type of cell. Codon optimization is a technique which is now well known and used in the design of synthetic genes. Different organisms preferentially utilize one or other of these different codons. By optimizing codons, it is possible to greatly increase expression levels of the particular protein in a selected cell type.
In accordance with the foregoing, embodiments of an isolated nucleic acid of the invention have been codon-optimized, which codon optimization is expressed as a Codon Adaptation Index (CAI), wherein such CAI for nucleic acids of the invention is at least 0.7, preferably at least 0.8, more preferably at least 0.9, and most preferably at least 0.97. For wild-type (non-optimized human BChE gene), the CAI is at or about 0.69. Such Codon Adaptation Index is determined according to methods known in the art by setting the quality value of the most frequently used codon for a given amino acid in the desired expression system to 100 with the remaining codons scaled accordingly (see Sharp and Li, Nucleic Acids Research, Vol. 15(3), 99. 1281-1295 (1987)). The CAI uses a reference set of highly expressed genes from a species to determine relative merit of each codon to calculate a score for a gene from the frequency of use of all codons in said gene. This index is useful for predicting the level of expression of a gene and indicate likely success of heterologous gene expression in a given cell system.
In some embodiments, the nucleic acids, for example, a DNA, of the present invention have also been optimized using additional parameters. For example, analysis of the wild-type human BChE gene has been found to contain an average G+C content of about 40% by determining the GC content in a 40 bp window centered about various nucleotide positions. Conversely, the GC content of nucleic acids according to the present invention, such as the codon optimized nucleic acids disclosed herein, have an average GC content of about 60%. For example, in producing nucleic acids of the present invention, very high or low GC content has been avoided, so that any GC content less than 30% or above 80% has been avoided.
The present invention relates to an isolated nucleic acid, DNA or RNA, that encodes a polypeptide having BChE enzyme activity (for example, using the well known Ellman assay—Ellman, G. L., et al, Biochem. Pharmacol., Vol. 7, pp. 88-95 (1961)), wherein the percentage of guanine plus cytosine (G+C) nucleotides in the coding region of said nucleic acid is greater than 40%, or is greater than 45%, or is greater than 50%, or is greater than 55%, or is at least 60%, or is greater than 60% but is less than 80%. In all cases, codon usage has been adapted to the codon bias of human (Homo sapiens) genes.
Specific embodiments of an isolated nucleic acid of the invention include nucleic acids comprising a nucleotide sequence having at least 80% identity, or at least 90% identity, preferably at least 95% identity, more preferably at least 98% identity to the nucleotide sequence of SEQ ID NO: 1 or the complement thereof. In a preferred embodiment, the nucleic acid of the invention comprises, more preferably consists essentially of, and most preferably consists of, the nucleotide sequence of SEQ ID NO: 1 or the complement thereof. In all cases, such nucleic acids, in addition to SEQ ID NO: 1 itself, meet the other requirements of the invention regarding GC content and/or CAI and like parameters.
In one embodiment, the BChE polypeptide encoded by the nucleic acid of the invention comprises, preferably consists of, amino acids 22-564 of SEQ ID NO: 2. In some embodiments, the encoded BChE polypeptide contains fewer than the number of amino acids in SEQ ID NO: 2 (i.e., is a truncated, variant or modified form of said sequence, such as the truncate shown as SEQ ID NO: 4 and/or in FIG. 3, with or without the signal sequence). Such modification may affect the overall 3-dimensional structure or shape of the resulting BChE enzyme. Consequently, the resulting encoded polypeptide does not readily form tetramers or even dimers, but remains in a monomeric state.
In accordance with the invention, such monomeric BChE polypeptides are achieved by producing a BChE polypeptide that differs from the native BChE of SEQ ID NO: 6, or is a variant of such polypeptide, such as a polypeptide having less than 100% identity to the sequence of SEQ ID NO: 2 or 4 or amino acids 22-564 of said sequences but retaining substantially all of the BChE enzyme activity of said polypeptide, or where one or more amino acids present in such polypeptides are either different or not included in said polypeptide, which is thus a variant or shortened or truncated polypeptide and which forms mostly, or all, or only, monomers.
For example, such a truncate commonly differs from native, or full length, BChE (such as SEQ ID NO: 6 starting at amino acid 17) in that a specific domain is not present. In a preferred embodiment, such a domain (referred to in the art as the WAT domain) comprises the C-terminal portion of the BChE polypeptide, preferably the C-terminal 20 to 40 amino acids of a native BChE, more preferably the C-terminal 25 to 35 amino acids of native BChE, most preferably the C-terminal 31 amino acids of such BChE. To produce a truncate within the invention, any amino acid segment after the tryptophan residue at residue 564 of SEQ ID NO: 2 (FIG. 1) may be deleted from the sequence of the mature protein. In one embodiment, said BChE polypeptide does not form monomers because all or a portion of said WAT domain has been deleted or because one or more amino acid substitutions have been introduced into said domain to render it non-functional for the purpose of inducing multimer formation of the resulting mature polypeptide.
For expression from DNA, this is accomplished by insertion of a termination codon following the codon encoding such tryptophan, either immediately following it or following a codon 3′ of said tryptophan codon so as to subsequently shorten the resulting encoded amino acid sequence of the BChE protein. Where the latter is to be synthesized by direct chemical synthesis, the sequence from the N-terminus of SEQ ID NO: 2 up to or exceeding the tryptophan at residue 564 is included in the synthetic product but not some, most or all of the amino acids C-terminal of said tryptophan to form a truncate. BChE truncates of the present invention may or may not include a signal sequence, such as that shown in FIG. 1, 2 or 3. Thus, a truncate of the mature polypeptide is contemplated by the invention. One such example would consist of amino acids 22-564 of SEQ ID NO: 4.
Such a monomer, especially one composed of such a truncate, has the advantage of a much better defined amino acid sequence and is capable of being synthesized in amounts up to 10 times, 20 times or even 40 times, or more, the amounts normally synthesized of the tetrameric form of BChE and at greatly reduced cost, thereby making it a much more desirable therapeutic agent from both a clinical and commercial viewpoint.
The sequence KAGFHRWNNYMMDWKNQFNDYTSKKESCVGL (SEQ ID NO: 7), located at amino acid residues 565-595 of SEQ ID NO: 2, has been found to be involved in formation of multimeric BChE molecules, such as in the dimerization and/or tetramerization of BChE (see, for example, Blong et al., supra, which contains additional structural information concerning such domains). By the deletion of part or all of this domain, most, if not all, of the resulting BChE product is rendered not capable of forming multimers and so remains in monomic form. In forming such a truncate of the invention, only so much of this domain need be removed to prevent dimer or tetramer formation from the synthesized monomer. In one embodiment, such as SEQ ID NO: 4 (FIG. 3), all of it is missing. For such a product, the expression of the monomer is higher, the purification and characterization easier, and the cost of the product substantially lower.
In one preferred embodiment, this truncated form of BChE is expressed in Per.C6 cells with the optimal clone selected. This truncated BChE has a long MRT, making it the preferable form as a drug product. In other embodiments, such truncate is also prepared by direct synthesis and other means, such as using recombinant cells, preferably mammalian cells, more preferably human cells, most preferably Per.C6 (or PerC6) cells, that achieve high levels of glycosylation and/or sialylation of a heterologous protein, or where synthesis, either in vitro or in vivo, is followed by in vitro glycosylation and/or sialylation.
In a preferred embodiment, a BChE truncate of the invention comprises the amino acid sequence of SEQ ID NO: 4 (shown in FIG. 3), which comprises a human signal sequence at the N-terminus.
A BChE truncate of the present invention is thus a BChE molecule with part or all of the WAT domain removed. Without a functioning WAT domain, the molecule does not form multimers, such as tetramers and/or dimers, but forms mostly, if not only, monomers. The selection of the truncation site, for example, after W at 564 (of SEQ ID NO: 2, for example) facilitates a more uniform C-terminal region. Use of Per.C6 cells coupled with the selection of high glycosylation and high sialylation clone(s) ensures long serum half-life and is a preferred embodiment of the invention. In sum, such a truncated BChE construct is simple, results in higher yield, longer serum half-life and a more homogeneous product, meeting all the requirements needed for an effective pharmaceutical agent.
The BChE truncate of the present invention can be produced by any means known in the art, including by direct chemical synthesis and the sequence of such truncate, where prepared by expression of an encoding DNA, need not be derived from a codon-optimized DNA. Standard references are available that contain procedures well known in the art of molecular biology and genetic engineering for producing the nucleic acids and polypeptides of the present invention. Useful references include Sambrook, et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y., (1989), Wu et al, Methods in Gene Biotechnology (CRC Press, New York, N.Y., 1997), and Recombinant Gene Expression Protocols, in Methods in Molecular Biology, Vol. 62, (Tuan, ed., Humana Press, Totowa, N.J., 1997), the disclosures of which are hereby incorporated by reference.
In another aspect, the present invention relates to a vector comprising a nucleic acid of the invention, as well as to a recombinant cell containing such a vector and expressing a BChE polypeptide by expressing a nucleic acid of the invention, preferably where that nucleic acid is present in a vector of the invention. The present invention also relates to a method of preparing such a polypeptide having BChE enzyme activity, comprising expressing the polypeptide from a recombinant cell as described herein, preferably where the polypeptide comprises the amino acid sequence of SEQ ID NO: 4 (for example, SEQ ID NO: 2), including where the polypeptide forms only monomers, for example, where such polypeptide does not contain portions of one or more domains that promote formation of such supra-molecular structures, including where the entire domain is absent or sequence altered.
As noted above, monomers were thought to be less active that tetramers but this is likely due to improper glycosylation. The present invention provides a codon optimized nucleic acid encoding BChE and appropriate glycosylation sites coupled with a cell line especially useful for expressing the properly glycosylated truncated molecule as a fully active monomer possessing good retention time.
A nucleic acid of the invention used to transform the cells useful in practicing the invention is a synthetic gene of which the nucleic acid of SEQ ID NO 1 is only a specific yet preferred example. Such nucleic acids include modified forms of SEQ ID NO: 1. The expression “modified form” refers to other nucleic acid sequences which encode BChE (including fragments or variants thereof) and have BChE enzyme activity but which utilize different codons, provided the requirement for the percentage GC content and other criteria recited in accordance with the invention are met. Suitable modified forms include those that comprise at least 80% identity, preferably at least 90% identity, more preferably at least 95% identity and even more preferably at least 98% identity to SEQ ID NO 1.
In one embodiment, the nucleic acid sequence of SEQ ID NO: 1 and/or SEQ ID NO: 3 have been codon-optimized for expression in human cells, such as Per.C6 cells (a fully characterized human cell line for use with recombinant adenoviral vectors (available from Crucell L.V., Leiden, The Netherlands). This cell line has the advantage of producing heterologous proteins in good yield. It has also been found to be free of prions, is easily transfected with exogenous genes and grows well in commercially available media free of animal and/or human derived proteins.
In addition to codon optimization, the different embodiments of the invention have been achieved by further optimizing the nucleic acids of the invention to avoid inclusion of polynucleotide sequence elements that would otherwise reduce expression of the nucleic acid, and subsequent synthesis of BChE. In particular said sequence elements may be selected from the group comprising; negative elements or repeat sequences, cis-acting motifs such as splice sites, internal TATA-boxes and ribosomal entry sites.
A TATA box (or TATA) site is well known in the art and generally represents a consensus sequence found in the promoter region of genes that are transcribed by the RNA polymerase II found in mammalian, such as human, cells. It is often located about 25 nucleotides upstream of the transcription start site (often having the sequence 5′ TATAAAA 3′ (SEQ ID NO: 11)). It is relevant in determining the initiation site for gene transcription. However, when such a site is present internally within the coding region of a gene it can adversely affect (i.e., slow) gene expression and is thus to be avoided where efficient high level expression is sought. Where possible, such sequences have been avoided in the nucleic acids of the present invention.
Gene expression is also slowed by other structural motifs found in coding regions of genes. One such motif is the chi-site, which can induce homologous recombination, thereby disrupting the cloned gene. For example, the enzyme RecBCD (a heterotrimeric helicase that initiates homologous recombination at double-stranded DNA breaks) can be modulated by the DNA sequence denoted “chi” (i.e., 5′-GCTGGTGG-3′ (SEQ ID NO: 8)). Such chi-sites have been avoided, where possible, in achieving the nucleic acids of the present invention, which preferably neither contain nor encode such chi sites.
In eukaryotes, the Kozak sequences 5′-ACCACCAUGG-3′ (SEQ ID NO: 9) or 5′-GCCACCAUGG-3 (SEQ ID NO: 10), which lie within a short 5′ untranslated region, direct translation of mRNA and are thus upstream of the transcription start site (the AUG codon that begins transcription). These sequences are effectively recognized by the ribosome as a translation start site and are different from the internal ribosomal binding site (RBS), which includes an internal ribosomal entry site (IRES) or the 5′cap of the mRNA molecule. The strength of the Kozak sequence can determine the extent of translation of the mRNA and thus the amount of protein produced. Internal ribosomal entry sites have been avoided, where feasible, in achieving the nucleic acids of the invention. However, the nucleic acids, or genetic constructs, of the invention, for purposes of expression from recombinant cells of the invention, preferably do encode a Kozak site upstream of the start site.
Protein-coding genes of mammals may also contain introns that ate involved in RNA splicing events that take place after transcription is complete but prior to translation at the ribosome. Sequences of such sites are known in the art and have, where feasible, been avoided in designing the sequences of the nucleic acids of the invention, which are made up mostly of coding sequence and are thus cDNA in nature. For example, such a splice site may contain a an almost invariant GT sequence at the 5′ end of the intron as part of a larger less conserved region. The 3′ splice site or splice acceptor site terminates the intron with an almost always present AG sequence. Upstream of this AG site is often found a sequence in pyrimidine content (i.e., C and T nucleotides). Such structural motifs are well known in the art and, where feasible, have been likewise avoided in achieving the nucleic acids, or DNA constructs, of the present invention.
Recombinant butyrylcholinesterase forms often exhibit variation in the type of sugar residues found within the different sugars attached to the molecule. Such variation can negatively affect the mean retention time (MRT) of the BChE molecule in vivo. Among the factors that can determine such variability are the number and arrangement of non-sialylated galactose and mannose residues as well as the host cell used to produce the glycosylated final product in BChE expression, since different expression systems may glycosylate the BChE molecule differently. Processes of in vitro glycosylation after synthesis have been attempted by those in the art to avoid such problems. For example, it has been shown that the stability of BChE is affected by capping of the terminal carbohydrate residues with sialic acid since uncapped galactose residues bind to receptors on hepatocytes and thereby clear the protein from the system. The present invention preferably utilizes the Per.C6 expression system so as to achieve as close a similarity to the native human glycosylation patent for BChE as is possible.

## Claims

1. An isolated nucleic acid that encodes a polypeptide having butyrylcholinesterase—(BChE) enzyme activity, wherein said nucleic acid has at least 90% identity to the nucleotide sequence of SEQ ID NO: 1, or the complement of said isolated nucleic acid.

2. The isolated nucleic acid of claim 1, wherein said percent identity is at least 95%.

3. The isolated nucleic acid of claim 1, wherein said percent identity is at least 98%.

4. The isolated nucleic acid of claim 1, wherein said nucleic acid is SEQ ID NO: 1.

5. The isolated nucleic acid of claim 1, wherein the percentage of guanine plus cytosine (G+C) nucleotides in the coding region of said nucleic acid is greater than 40% but not greater than 80%.

6. The isolated nucleic acid of claim 1, wherein the percentage of guanine plus cytosine (G+C) nucleotides in the coding region of said nucleic acid is greater than 45% but not greater than 80%.

7. The isolated nucleic acid of claim 1, wherein the percentage of guanine plus cytosine (G+C) nucleotides in the coding region of said nucleic acid is greater than 50% but not greater than 80%.

8. The isolated nucleic acid of claim 1, wherein the percentage of guanine plus cytosine (G+C) nucleotides in the coding region of said nucleic acid is greater than 55% but not greater than 80%.

9. The isolated nucleic acid of claim 1, wherein the percentage of guanine plus cytosine (G+C) nucleotides in the coding region of said nucleic acid is greater than 60% but not greater than 80%.

10. The isolated nucleic acid of claim 1, wherein said nucleic acid does not contain or encode an internal TATA-box.

11. The isolated nucleic acid of claim 1, wherein said nucleic acid encodes one or more sialylation sites on said polypeptide.

12. The isolated nucleic acid of claim 1, wherein said nucleic acid does not contain or encode an internal ribosomal entry site.

13. The isolated nucleic acid of claim 1, wherein said nucleic acid does not contain or encode a splice donor or acceptor site.

14. The isolated nucleic acid of claim 1, wherein said nucleic acid contains or encodes at least one Kozak sequence upstream of the start site.

15. The isolated nucleic acid of claim 1, wherein the sequence of said isolated nucleic acid has a Codon Adaptation Index (CAI) of at least 0.7.

16. The isolated nucleic acid of claim 15, wherein said CAI is at least 0.8.

17. The isolated nucleic acid of claim 15, wherein said CAI is at least 0.9.

18. A vector comprising the nucleic acid of claim 1.

19. A recombinant cell containing the vector of claim 18.

20. The recombinant cell of claim 19, wherein said cell is a mammalian cell.

21. The recombinant cell of claim 19, wherein said cell is a human cell.

22. The recombinant cell of claim 19, wherein said cell is a Per.C6 cell.

23. A method of preparing a polypeptide having BChE enzyme activity, comprising expressing said polypeptide from the cell of claim 19.

24. The method of claim 23, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 2.

25. The isolated nucleic acid of claim 1, wherein said isolated nucleic acid is a DNA.

26. The isolated nucleic acid of claim 1, wherein said isolated nucleic acid is a cDNA.

27. The isolated nucleic acid of claim 1, wherein said isolated nucleic acid is an RNA.