# Patent

## Title
Droplet-based nucleic acid amplification in a temperature gradient

## Abstract

A method of amplifying a nucleic acid, the method comprising cycling an amplification-ready droplet through a temperature gradient to locations within the temperature gradient suitable for effecting steps in an amplification reaction.

## Background
1 GOVERNMENT INTEREST
This invention was made with government support under AI066590-02, awarded by the National Institutes of Health of the United States. The United States Government has certain rights in the invention.


2 BACKGROUND
Compared to stationary formats, “flow-through” polymerase chain reaction (PCR) offers advantages in terms of speed, sample throughput, ease of thermal control and integration with other microfluidic operations. However, current flow-through PCR approaches that are based on continuous flow of reactants in fixed channels lack flexibility because the available thermal profiles are determined by the physical layout of the channels. There is a need for droplet-based approaches to flow-through PCR.
3 SUMMARY OF THE INVENTION
The invention provides a method of amplifying a nucleic acid. The method involves cycling an amplification-ready droplet through a temperature gradient to locations within the temperature gradient suitable for effecting steps in an amplification reaction. The cycling may be accomplished using droplet operations on a droplet operations surface mediated by electrodes associated with the surface. The droplet is shuttled through an oil-based filler fluid on a droplet actuator to the locations.
The locations may include locations having temperatures suitable for: (a) adjusting the droplet to a temperature selected to denature double stranded nucleic acid in the droplet into single-stranded DNA (typically about 90-100° C.); and (b) adjusting the droplet temperature selected to permit primers to anneal to their complementary sequences on the nucleic acid template strands (typically about 50-75° C.); and (c) adjusting the droplet temperature selected to facilitate extension of the double stranded segment of the nucleic acid to be extended by incorporation of additional nucleotides (typically about 70-75° C.).
In some cases, the droplet is shuttled or transported to different locations within the temperature gradient to identify improved amplification patterns based on the results of varying the locations. For example, the method may be used to independently varying anneal, extension and/or denaturation location within the thermal gradient to identify an improved anneal location or temperature. The method may further involve executing an improved PCR amplification protocol using the newly identified locations. In some cases, the improvement may involve minimizing denaturation and annealing/extension times to achieve minimum analysis time.
The temperature gradient may, for example, be established using a heating element arranged to heat filler fluid at a locus of an electrode path. In other cases, the temperature gradient is established (a) using a heating element arranged to heat filler fluid at a first locus of an electrode array or path; and (b) a heat sink element arranged to heat filler fluid at a second locus of an electrode array or path. Any number of heating elements and/or heat sinks may be used.
In some cases, the method also involves mapping the temperature gradient of the droplet actuator. Further, the mapped temperature gradient may be associated with droplet operations electrodes at specific temperatures in order to select locations within the gradient for conducting the various amplification steps.
4 DEFINITIONS
As used herein, the following terms have the meanings indicated.
“Activate” with reference to one or more electrodes means effecting a change in the electrical state of the one or more electrodes which results in a droplet operation.
“Droplet” means a volume of liquid on a droplet actuator that is at least partially bounded by filler fluid. For example, a droplet may be completely surrounded by filler fluid or may be bounded by filler fluid and one or more surfaces of the droplet actuator. Droplets may take a wide variety of shapes; nonlimiting examples include generally disc shaped, slug shaped, truncated sphere, ellipsoid, spherical, partially compressed sphere, hemispherical, ovoid, cylindrical, and various shapes formed during droplet operations, such as merging or splitting or formed as a result of contact of such shapes with one or more surfaces of a droplet actuator.
“Droplet operation” means any manipulation of a droplet on a droplet actuator. A droplet operation may, for example, include: loading a droplet into the droplet actuator; dispensing one or more droplets from a source droplet; splitting, separating or dividing a droplet into two or more droplets; transporting a droplet from one location to another in any direction; merging or combining two or more droplets into a single droplet; diluting a droplet; mixing a droplet; agitating a droplet; deforming a droplet; retaining a droplet in position; incubating a droplet; heating a droplet; vaporizing a droplet; cooling a droplet; disposing of a droplet; transporting a droplet out of a droplet actuator; other droplet operations described herein; and/or any combination of the foregoing. The terms “merge,” “merging,” “combine,” “combining” and the like are used to describe the creation of one droplet from two or more droplets. It should be understood that when such a term is used in reference to two or more droplets, any combination of droplet operations that are sufficient to result in the combination of the two or more droplets into one droplet may be used. For example, “merging droplet A with droplet B,” can be achieved by transporting droplet A into contact with a stationary droplet B, transporting droplet B into contact with a stationary droplet A, or transporting droplets A and B into contact with each other. The terms “splitting,” “separating” and “dividing” are not intended to imply any particular outcome with respect to size of the resulting droplets (i.e., the size of the resulting droplets can be the same or different) or number of resulting droplets (the number of resulting droplets may be 2, 3, 4, 5 or more). The term “mixing” refers to droplet operations which result in more homogenous distribution of one or more components within a droplet. Examples of “loading” droplet operations include microdialysis loading, pressure assisted loading, robotic loading, passive loading, and pipette loading. Droplet operations may be electrode-mediated. In some cases, droplet operations are further facilitated by the use of hydrophilic and/or hydrophobic regions on surfaces and/or by physical obstacles.
The terms “top” and “bottom,” when used, e.g., to refer to the top and bottom substrates of the droplet actuator, are used for convenience only; the droplet actuator is functional regardless of its position in space.
When a given component, such as a layer, region or substrate, is referred to herein as being disposed or formed “on” another component, that given component can be directly on the other component or, alternatively, intervening components (for example, one or more coatings, layers, interlayers, electrodes or contacts) can also be present. It will be further understood that the terms “disposed on” and “formed on” are used interchangeably to describe how a given component is positioned or situated in relation to another component. Hence, the terms “disposed on” and “formed on” are not intended to introduce any limitations relating to particular methods of material transport, deposition, or fabrication.
When a liquid in any form (e.g., a droplet or a continuous body, whether moving or stationary) is described as being “on”, “at”, or “over” an electrode, array, matrix or surface, such liquid could be either in direct contact with the electrode/array/matrix/surface, or could be in contact with one or more layers or films that are interposed between the liquid and the electrode/array/matrix/surface.
When a droplet is described as being “on” or “loaded on” a droplet actuator, it should be understood that the droplet is arranged on the droplet actuator in a manner which facilitates using the droplet actuator to conduct droplet operations on the droplet, the droplet is arranged on the droplet actuator in a manner which facilitates sensing of a property of or a signal from the droplet, and/or the droplet has been subjected to a droplet operation on the droplet actuator, e.g., a layer of filler fluid.


5 DESCRIPTION
Digital microfluidic systems that are based on the electrowetting effect allow individual droplets to be rapidly and programmably transported between discrete positions on a surface using an array of electrodes. The invention provides a PCR chip assembly that has a thermal gradient that is generated across the surface of an electrode array. The thermal gradient permits thermal cycling to be performed by transporting individual droplets between different positions within the thermal gradient. Other Embodiments of the invention will be apparent from the ensuing discussion.
FIG. 1 illustrates a perspective view of a PCR chip assembly 100, which includes a digital microfluidic PCR chip and top plate. The invention provides a PCR droplet actuator that has a thermal gradient that is generated across the surface of the electrode array, which allows thermal cycling to be performed by transporting individual droplets between different positions within the thermal gradient.
PCR chip assembly 100 may be used to perform flow-through real-time PCR in microfluidic droplets by shuttling droplets along electrode path 105 along the temperature gradient to locations within the gradient suitable for conducting various steps in the amplification process, such as annealing, extension, and denaturation. Because the flow is programmable, thermal programs may be highly flexible, while the rapidity of droplet transport allows rapid cycling of temperatures. In one example, 40 cycles of real-time PCR is performed in less than about 5, 4, 3, or 2 minutes.
Referring again to FIG. 1, microfluidic devices, such as PCR chip assembly 100, may be fabricated by patterning chrome electrodes on glass slides using standard photolithographic techniques. A spacer/gasket (e.g., plastic) may be sandwiched between the patterned plate and a second glass containing an indium-tin-oxide (ITO) ground electrode. In one example, the electrode array may consist of a single linear array of 20 square electrodes at about 1.5 mm pitch. The unit droplet volume may, for example, be about  ̃100,000, 10,000, 2000, 1500, 1000 or 500 nL or less or volumes intermediate to such volumes, and other parameters of the droplet actuator, such electrode size, pitch and gap height may be adjusted accordingly.
FIG. 2 illustrates a perspective view of a custom-made chip connector/heater assembly 200, which is an example mechanism for providing thermal control to, for example, PCR chip assembly 100 of FIG. 1. In one example, chip connector/heater assembly 200 includes an aluminum plate that contains two separate heater blocks each contacting a different area of PCR chip assembly 100 and each connected to independently controlled Peltier thermoelectric devices. A thermistor embedded in each block may be used for PID temperature control of the block. The internal temperature gradient of the chip may be calibrated with respect to the heater block and spatially mapped using both miniature thermocouples and melting point analysis of pure organic compounds.
Referring again to FIGS. 1 and 2, real-time fluorescence detection may be performed using a conventional fluorescence microscope, e.g., using a SYBR Green reaction. During the fluorescence detection operation, chip connector/heater assembly 200 may be mounted on the microscope stage and the fluorescence inside the droplet may be quantified using a sensitive photomultiplier tube (PMT). As an example, the PCR model assay was used to amplify a 273 bp fragment of Candida albicans 18S ribosomal RNA gene. The final reagent concentrations in the PCR reaction were be about 50 mM KCl, about 20 mM Tris-HCl pH 8.4, about 200 μM each dNTPs, about 25 U/mL iTaq polymerase, about 3 mM MgCl2, and about 0.2 M oligonucleotide primers.
FIG. 3 illustrates a top view of an electrode array 300 that shows the relative location and position of the Peltier heater and PCR droplet (top) and thermal map of the electrode array (bottom). On-chip PCR experiments may be performed, for example, by manually pipetting or otherwise loading a 1000 mL reaction droplet into a chip that is filled with hexadecane in order to prevent evaporation. The droplet may then be cyclically transported between the 94° C. and 60° C. positions according to a user program. Additionally, the apparatus may be mounted on a fluorescence microscope that is focused on an electrode in the “extension” region to allow fluorescence measurement at the end of each cycle.
FIG. 4 illustrates a plot 400 of a comparison of an example of real-time PCR results for the chip-based system of the invention and a commercial benchtop thermocycler (e.g., BioRad iQ5) for varying concentrations of starting DNA. The results may be validated by agarose gel electrophoresis of the droplets that are collected at the end of each real-time experiment.
The time required for the sample droplet to transit between any two electrode positions of, for example, electrode array 300 of FIG. 3 is less than about 1 second, which allows much faster thermal programs to be performed on-chip than can be performed on the commercial system. An optimization may be performed using the chip system in which both the denaturation and annealing/extension times may be systematically reduced in order to determine the minimum analysis time. A different primer set that produces an 80-bp amplicon of the same gene may be used in these experiments, but the primer length remains substantially constant so that specificity is not lost in the transition.
FIGS. 5A, 5B, and 5C illustrate a plot 500, a plot 510, and a plot 520, respectively, which shows a progression of speed optimization operations. Plots 500, 510, and 520 show real-time curves for optimization of time held at both the denaturing and annealing/extension temperature. The target amplicon was about 80-bp in length, allowing for a shorter extension period compared to the 273-bp amplicon. The results indicate that the fastest successful condition for amplification of the 80-bp target from a genomic template was 1.5 s @ 94° C. and 4 s @ 60° C. Combined with 2 s total transport time, this results in 7.5 s per cycle with 40 cycles of PCR performed in 5 minutes.
Aspects of performing rapid, programmable, real-time PCR using digital microfluidics include, but are not limited to (1) rapid thermal cycling—due to the low thermal mass and speed at which droplets can be transported; (2) precise control—programmably position droplets anywhere within a gradient; (3) flexibility—modify or change thermal programs in real-time through software; (4) scalability—multiple samples can be processed in parallel (with independent thermal programs if required); (5) integration—PCR can be integrated with other microfluidic operations (i.e. sample prep) on a single platform, (6) energy efficiency—heaters are not cycled and electrowetting consumes very little power, which is ideal for portable applications; and (7) multiplexed detection—multiple droplets can be scheduled for detection at a single fixed detection spot.
5.1 Droplet Microactuator
For examples of droplet microactuator architectures and PCR reagents and protocols that are suitable for use with the present invention, see U.S. Pat. No. 6,911,132, entitled, “Apparatus for Manipulating Droplets by Electrowetting-Based Techniques,” issued on Jun. 28, 2005 to Pamula et al.; U.S. patent application Ser. No. 11/343,284, entitled, “Apparatuses and Methods for Manipulating Droplets on a Printed Circuit Board,” filed on filed on Jan. 30, 2006; U.S. Pat. No. 6,773,566, entitled, “Electrostatic Actuators for Microfluidics and Methods for Using Same,” issued on Aug. 10, 2004 and U.S. Pat. No. 6,565,727, entitled, “Actuators for Microfluidics Without Moving Parts,” issued on Jan. 24, 2000, both to Shenderov et al.; Pollack et al., International Patent Application No. PCT/US 06/47486, entitled, “Droplet-Based Biochemistry,” filed on Dec. 11, 2006, the disclosures of which are incorporated herein by reference. The term “chip” is used herein to refer to a “droplet actuator” chip.
5.2 Fluids
For examples of fluids that may be subjected to droplet operations using the approach of the invention, see the patents listed in section 5.1, especially International Patent Application No. PCT/US 06/47486, entitled, “Droplet-Based Biochemistry,” filed on Dec. 11, 2006. In some embodiments, the fluid that is loaded includes a biological sample, such as whole blood, lymphatic fluid, serum, plasma, sweat, tear, saliva, sputum, cerebrospinal fluid, amniotic fluid, seminal fluid, vaginal excretion, serous fluid, synovial fluid, pericardial fluid, peritoneal fluid, pleural fluid, transudates, exudates, cystic fluid, bile, urine, gastric fluid, intestinal fluid, fecal samples, fluidized tissues, fluidized organisms, biological swabs and biological washes. In some embodiment, the fluid that is loaded includes a reagent, such as water, deionized water, saline solutions, acidic solutions, basic solutions, detergent solutions and/or buffers. In some embodiments, the fluid that is loaded includes a reagent, such as a reagent for a biochemical protocol, such as a nucleic acid amplification protocol, an affinity-based assay protocol, a sequencing protocol, and/or a protocol for analyses of biological fluids.
5.3 Filler Fluids
The gap is typically filled with a filler fluid. The filler fluid may, for example, be a low-viscosity oil, such as silicone oil. Other examples of filler fluids are provided in International Patent Application No. PCT/US 06/47486, entitled, “Droplet-Based Biochemistry,” filed on Dec. 11, 2006.

## Claims

1. A method of amplifying a nucleic acid, the method comprising:
(a) providing an amplification-ready droplet loaded operations gap of a droplet actuator, wherein the droplet actuator comprises:
(i) two substrates separated to provide the droplet operations gap therebetween;
(ii) electrodes associated with the substrates and arranged for transporting droplets in the droplet operations gap by electrowetting, the electrodes including an electrode path comprising electrodes arranged to transport droplets along the electrode path; and
(iii) an oil-based filler fluid in the droplet operations gap at least partially surrounding the amplification-ready droplet;

(b) applying heat to the droplet actuator to establish a temperature gradient of gradually varying temperature in the droplet operations gap, wherein the temperature gradient is established using a heating element arranged to heat filler fluid at a locus of the electrode path; and
(c) cycling the amplification-ready droplet by using the electrodes to transport the amplification-ready droplet through the temperature gradient to locations within the temperature gradient having temperatures suitable for effecting steps in an amplification reaction, wherein the droplet is shuttled along the electrode path to different locations within the temperature gradient, and improved amplification patterns based on the results of varying the locations are identified.

2. The method of claim 1 wherein the droplet is shuttled through the oil-based filler fluid along the electrode path to the locations by using the electrodes to conduct electrowetting-mediated droplet operations on the droplet.

3. The method of claim 1 wherein the locations comprise locations having temperatures suitable for:
(a) adjusting the droplet to a temperature selected to denature double stranded nucleic acid in the droplet into single-stranded DNA;
(b) adjusting the droplet temperature selected to permit primers to anneal to their complementary sequences on the nucleic acid template strands; and
(c) adjusting the droplet temperature selected to facilitate extension of the double stranded segment of the nucleic acid to be extended by incorporation of additional nucleotides.

4. The method of claim 1 comprising independently varying anneal location to identify an improved anneal location or temperature.

5. The method of claim 1 comprising independently varying extension location to identify an improved extension location or temperature.

6. The method of claim 1 comprising independently varying denaturation location to identify an improved denaturation location or temperature.

7. The method of claim 1 further comprising executing an improved PCR amplification protocol using the newly identified locations.

8. The method of claim 1 further comprising minimizing denaturation and annealing/extension times to reduce analysis time.

9. The method of claim 1 wherein the temperature gradient is established comprising:
(a) using a heating element arranged to heat filler fluid at a first locus of the electrode path; and
(b) a heat sink element arranged to heat filler fluid at a second locus of the electrode path.

10. The method of claim 1 further comprising mapping the temperature gradient.

11. The method of claim 1 further comprising mapping the temperature gradient to specific electrodes of the electrode path.