### Page 1 of 51 OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION 

### UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON, D.C. 20460

May 2 9

, 201 4

PC Code: 078906 MEMORANDUM DP Barcode: D 417911

SUBJECT: Registration Review: Preliminary Problem Formulation for Environmental Fate,

Ecological Risk, Endangered Species, and Human Health Drinking Water Exposure Assessments for Bi spyribac

- Sodium

FROM: Edward Scollon, Ph.D., Toxicologist

Cheryl Sutton, Ph.D., Environmental Scientist Environmental Risk Branch IV Environmental Fate and Effects Division (7507P)

REVIEWED BY: Jim Carleton, Ph.D., Senior Fate Scientist

Thomas Ste eger, Ph.D., Senior Advisor Environmental Risk Branch IV Environmental Fate and Effects Division (7507P)

THROUGH: Sarah Winfield

, Acting Branch Chief

Environmental Risk Branch IV Environmental Fate and Effects Division (7507P)

TO: Moana Appleyard , Che mical Review Manager

Linda Arrington

, Branch Chief Risk Management and Implementation Branch V Pesticide Re

- evaluation Division (7508P)

1. Executive summary

The Environmental Fate and Effects Division (EFED) has completed the preliminary problem formu lation for the ecological risk, environmental fate, endangered species, and drinking water assessments to be conducted as part of the R egistration R eview of bispyribac

- sodium ( CAS No. 125401

- 92

- 5 ; PC code 078906).

Th is problem formulation describes the methods planned to be used during the completion of drinking water and ecological risk assessments in support of R egistration R eview and provides an overview of the environmental fate, ecological effects, and potential risks associated with the use of bisp yribac

- sodium as well as uncertainties unique to risk assessment of bispyribac

- sodium . This document also identifies

### Page 2 of 51

additional studies that would be beneficial to the conduct of an ecological risk assessment. Major findings include:

 The fate database i s incomplete based on the environmental fate data requirements outlined in the Code of Federal Regulations Title 40 Part 158 (40CFR158) Subpart N 1 

### . Data are needed for three additional soils for aerobic soil metabolism (835.4100)

, and for two sediments fo r aerobic aquatic metabolism (835.4300) . The previously submitted aerobic aquatic metabolism study was downgraded to unacceptable based on insufficient study duration. The study was not conducted long enough to reach a half

- life, nor was it conducted for at least 100 days. Because the pesticide has a direct water application, these data are necessary for reducing uncertainty in the understanding of the fate of the compound in aquatic environments.

 

### A minimum retreatment interval is not spec ifically stated as such for each of the multiple rate options stated for use on athletic fields and turf.

 

### The ecological database is also incomplete . Data are needed for a honeybee larval toxicity study OECD 237/non

- guideline

.

2. I ntroduction

Bispyribac

- sodium is a post

- emerg ence pyrimidinyloxybenzoic acid herbicide formulated as a wettable powder or water soluble granule, packaged in water soluble bags

, used to control blue grass and broadleaf weeds. This herbicide is in the Weed Science Society of America (WSSA) group 2 and has a mode of action of inhibiting acetolac e tate synthase (ALS inhibitor). Bispyribac

- sodium was registered in the U.S. on September 24, 2001. In addition to the technical product, there are four Section 3 active registrations and six Section 24c S pecial L ocal N eed (SLN) registrations. End use products include Regiment ®



### , Velocity ® 

### and Tradewind TM 

### Herbicide s : single chemical products containing 80% a.i. A dditional information is presented in Appendix B

. EFED evaluated the most recent ecological risk a ssessments for bispyribac

- sodium in association with any available updated toxicity, exposure, and usage information to determine if sufficient data are available and if further updates are needed to support Registration Review. Recent EFED risk

assessmen ts include an ecological risk assessment and a drinking water assessment for the new aquatic

use (direct application to water) in 2010 (USEPA 2010 a and 2010b ). No new uses have been assessed since that time

. In addition, EFED considered the latest Agency science policies and risk assessment methodologies.

3. Use Characterization

Based on information provided in the Biological and Economic Analysis Division ( BEAD ) C hemical P rofile (BCP ; USEPA 2013 a ) for Registration Review, bispyribac

- sodium is commonly fo rmulated as 

### a wettable powder or water soluble granule, packaged in water soluble bags, and applied using ground or aerial sprayers. It is registered for use on rice

. Bispyribac

- sodium also has non

- agricultural uses and is registered for use on ornamenta l lawns and turf, including golf courses and sod farms, as well

1 CFR. 201 3. Title 40 (Protection of the Environment), Chapter 1 (Environmental Protection Agency), Subchapter E (Pesticide Programs), Part 158 (Data Requirements for Pesticides), Subpart N (Environmental Fate) http://www.ecfr.gov/cgi

- bin/text

idx?c=ecfr&SID=ff5c7fef3645e7cd5abceca0b597bccb&tpl=/ecfrbrowse/Title40/40cfr158_main_02.tpl

### Page 3 of 51

as aquatic sites such as canals, drainage ditches, ma r shes, lakes, ponds, and reservoirs. The Screening Level Usage Analysis (SL UA; USEPA 20 13b ) conducted by BEAD estimates the following agric ultural use during 200 4

- 201 1 : rice at 4000 lbs

. However, based on data for the years 2003

- 2012, the average annual use ( i.e ., 3,510 lbs) during the first half of that time period (2003

- 2007) was lower than the average annual use ( i.e ., 5,196 lbs) for the second half (2008

- 2012) , indicating a trend of increasing use (USEPA 2013a) . Additionally, the average annual use rates and average annual areas treated both increased (approximately by 50% and 20%, respectively) as well during the latter half of the tim e period. Based only on available agricultural use data, bispyribac

- sodium is used mainly in the states of California, Arkansas, Texas and Louisiana, with lower

- level uses in Mississippi and Missouri. Use e stimates are not available for the registered no n

- agricultural uses.

The BCP , located in the docket, lists the use patterns associated with the active registrations of bispyribac

- sodium . EFED will rely on the BCP when estimating environmental exposure s that result from these registered uses. Addition ally, in order to be protective, EFED will utilize the use information that result s in maximum exposure estimates for a given use pattern for the drinking water and ecological risk assessment s

. If the labels (and consequently the B CP ) do not include infor mation needed to estimate
exposure [ e.g ., the maximum single application rate, the application interval, the annual maximum rate allowed (lbs a.i./year), and application method ]
, EFED will make conservative assumptions. T o that end,

a minimum retreatment interval is not specifically stated as such for each of the multiple rate options stated on the label for uses on athletic fields and turf (EPA Registration Number 59639

- 136)

. I n the absence of this information, EFED will use conservative estimates of use and exposure.

4. Conclusions from Previous Risk Ass essments

a. Ecological Risk Assessment

The Agency recently conducted a screening

- level ecological risk assessment (ERA) for a new aquatic use in 2010 ( USEPA 2010a) that serve s as a basis for this problem formulation

. Proposed new u ses include d i ) an aquati c surface weed treatment via aerial or broadcast application and ii ) a subsurface treatment for submerged weeds using a weighted trailing hose to inject bispyribac

- sodium into the lower portions of the water column. T h e ERA indicate d that the proposed new aquatic surface use of bispyribac

- sodium to control aquatic weeds has the potential for direct adverse effects on both Federally listed threatened/endangered (hereafter referred to as “listed”) and non

- listed aquatic and terrestrial plant species ( i.e ., li sted non

- vascular aquatic plants; listed and non

- listed aquatic vascular plants; and listed and non

- listed terrestrial plants). The proposed subsurface applications are not expected to adversely impact terrestrial plants (since spray drift is not expected with subsurface applications); however, non

target aquatic plants may be at risk from this use. Direct adverse effects to terrestrial animals ( i.e
., terrestrial invertebrates, birds, terrestrial

- phase amphibian s , reptiles and mammals ) from acute or chron ic
exposure are not expected. Furthermore, direct adverse effects to aquatic animals ( i.e
., fish, aquatic

- phase amphibians and aquatic invertebrates) from acute or chronic exposure are not expected. Although no direct adverse effects in terrestrial and a quatic animals from bispyribac

- sodium use are expected, given the potential for direct effects on both terrestrial and aquatic plants, indirect effects to all listed animals and plants are considered possible given potential reductions in primary productiv ity and habitat

. For subsurface injection for control of aquatic weeds, the maximum proposed application rate for the aquatic use

, based on the proposed TradewindTM Herbicide label

, is that which will produce an initial maximum treatment concentration of 45 μg a.i./L in the water column [ per label language, subsurface applications must be made such that the actual aqueous concentration may not exceed 45 μg a.i./L ( ppb) at any point in time].

### Page 4 of 51

The major environmental fate and ecological risk conclusions iden tified in the most recent ERA are summarized in Table s 1 a and 1b

.

Table 1 a . Risk Concerns Identified for Bispyribac

- Sodium for Direct and Indirect Effects

Birds Mammals Terr. Plants Terr.

Inverts Fish Aquatic Inverts Aquatic

Plants Non

- listed and Liste d Species Direct No No Yes a No No No Yes b Indirect c Yes a Yes a

- Yes a Yes b Yes b

-

a LOCs for were exceeded in a screening level assessmnt using the TerrPlant model for ground and aerial surface applications

b LOCs for were exceeded in a screening level ass essmnt using the TerrPlant model for surface and subsurface applications

c Given the potential for effects on aquatic and that aquatic plants serve as the basis of primary productivity in aquatic comm unities, reductions in or loss of primary productivity is likely to result in indirect effects on other aquatic taxa.

- Indirect risks are implied when direct risks have been determined Yes = at least one LOC has been exceeded in previous assessments; No = LOCs were not exceeded in previous assessments.



### Table 1b

. Additional Risk Concerns Identified for Bispyribac

- Sodium

BCF Groundwater

Contamination Persistence Degradates of Concern No Yes Yes Yes



### b. Drinking Water Exposure Assessments

A drinking water exposure assessment (DWA) was conducted for bispyribac

- sodi um in 2010 (USEPA, 20 10 ) as part of a Section 3 new use assessment for the direct a pplication to water

.

Estimated d rinking water concentration s (EDWC ) were based on bispyribac

- s odium applied directly to surface water bodies to attain the maximum label target rate of 45 μg/L (parts per billion; ppb) for subsurface injection (USEPA 2010b)

. The application rate for surface application to water would result in a lower concentration. Thus, 45 ppb was recommended as the maximum surface water EDWC for both

s urface application and subsurface injection direct aquatic uses. Prior to that DWA, an assessment was conducted for a new use on turfgrass ( USEPA 2004 a ). In that assessment, the respective acute, chronic, and long

- term chronic (cancer) surface water EDWCs were 4.9, 1.5, and 0.8 ppb. The groundwater EDWC was 0.14 ppb

. All EDWCs are based on the parent compound only, as there are no other residue s of concern for drinking water (USEPA 2004a).

c. Clean Water Act Programs

B ispyribac

- sodium is not specifically identified as a cause of impairment for any water bodies listed as impaired under section 303(d) of the Clean Water Act. No Total Maximum Daily Load s (TMDL s ) or water quality criteria have been developed for bispyribac

- sodium

. Aquatic benchmarks have re cently been established for bispyribac

- sodium , and will be posted in the future at http://www.epa.gov/oppefed1/ecorisk_ders/aquatic_life_benchmark.htm

.
5. Environmental Fate and Transport

Based on the submitted data, bispyribac

- sodium is a moderately persistent (Goring classification ; Goring et al ., 1975 ), moderately mobile (FAO classification ; FAO, 2000 ) compound . In soil, bispyribac

- sodium is stable to abiotic degradation . The primary degradation pathways in soil and aquatic environments are

### Page 5 of 51

aerobic and anaerobic metabolism, with the formation of multiple major metabolites and unextracted residues , and mineralization to carbon dioxide (see Table 3 ).

When applied direct ly to water, bispyribac

- sodium is essentially stable to abiotic degradation by hydrolysis and aqueous photolysis , although hydrolysis occurs slowly at acidic pH level s

. It is subject to microbial degradation under aerobic and anaerobic conditions, with sl ower degradation observed in anaerobic aquatic environments (half

- lives of 88

- 109 days) relative to a single aero bic soil (half

- life of 62 days). In the single aerobic soil metabolism study submitted, up to 57% of the applied was mineralized to CO 2, 

### and h igh levels of unextracted residues were formed (26

- 54% ) by study termination. Recovery of degradates from initially unextracted residues was only accomplished with harsh base extraction. Additional aerobic soil metabolism data are needed to adequately de termine the fate of this compound in soil, and a cceptable aerobic aquatic metabolism data have not been submitted.

Soil

- water partition coefficients for the parent compound (K d 

### ) range from 0.60

– 2.0 L/kg and organic

carbon

- normalized partition coefficien ts (K oc 

### ) range from 143

- 604 L/kg. However, when the parent is present at typical ambient pH level s in the environment , it will be present mainly as an anion (pKa = 1.9)

, and its mobility in soil will be greater than indicated by the K oc 

### . Mobility data fo r the major degradates ( DesMe

- 2023, Me 2 

### Ba, MeBa, DesMe180, an d Bx

- 180 ; Table 3 ) have not been submitted to the Agency

. However, because the se degradates are ionizable compounds, K oc 

### data may not be useful at determining mobility in soil.

In an aquatic fi eld dissipation study conducted in Florida, bispyribac

- sodium dissipated with a half

- life of 25 days after being directly applied to the surface of a lentic pond . It remained mainly in the water column and was only detected once in the sediment. The majo r degradate, and only degradate monitored in the study, was DesMe

- 2023, which was present in the water phase and dissipated with a half

- life of 36 days. DesMe

- 2023 was not detected in sediment samples. In aquatic field dissipation studies conducted
at ri ce pa dd y sites in Arkansas and Louisiana, bispyribac

- sodium dissipated in each study with a half

- life of 11 days in the sediment (0

- 4 cm) and was only detected at very low levels (near the limit of quantification ( LOQ ): 0.001 ppm ) in the water. A total sy stem half

- life is not available for either study but is assumed to be similar to that determined for the sediment. Both the LA and AR studies were for application to dry seeded rice, followed by flooding one day post

- treatment. A fourth aquatic field dis sipation study ( MRID 44889232), conducted in California, remains classified as unacceptable.

In the environment, because it is an ionizable compound (pK a 

### of approximately 1.9, indicating that half of the bispyribac

- sodium molecules will be ionized at or a bove a pH of 1.9, with increasing percentage of the anionic form as pH increases), it should be present primarily as an anion. Based on its mobility and persistence, t here is a potential for both surface runoff and leaching to groundwater , and therefore t here is a potential groundwater concern

. Bispyribac

- sodium is included in the California Department of Pesticide Regulation ’s ( CA DPR) Grou nd Water Protection List (GWPL ; Title 3 California Code of Regulation (CCR), Division 6, § 6800(b) ), which lists pes ticides that have been designated as having the pot ential to pollute groundwater and are to be monitored by CA DPR.

While there are no bioconcentration data, it is not expected to bioaccumulate in fish due to its hydrophilicity ( i.e ., low log octanol

- wate r partition coefficient; log K ow 

### = - 

### 1.02) at most environmental pH values.

A selection of environmental fate and transport properties of bispyribac

- sodium is summarized in Table 2

.

### Page 6 of 51

Table 2

. Chemical Properties and Environmental Fate Parameters of Bispyribac

- sodium

Parameter Value Description /Comments Source

Selected Physical/Chemical Parameters
Molecular weight 452.4 g/mol

− DP Barcode D267285

Vapor pressure (20°C) 1 x 10

- 7 

### Torr Not expected to volatilize f

rom dry surfaces

. DP Barcode

D267285

Aqueous solubility (25  C ; pH 7 ) 73,300 mg/L Readily soluble. DP Barcode D267285

Dissociation Constant pKa (25 o 

### C) 1.9 Estimated

. Compound will exist as an anion at environmental pHs. Toxnet

Log octanol

- to

- water part ition coefficient (Log K OW 

### ) - 1.03 Experimental database value. EPISuite

Persistence Hydrolysis half

- life (22  C) pH 5: 88 days

pH 7: stable pH9: stable Does not hydrolyze at

neutral and alkaline pHs; hydrolyzes very slowly at acidic pHs. MRID 448892

- 26

Aqueous photolysis half

- life ( pH 7; 25  C) Stable Does not photolyze in
water. MRID 448892

- 27

Soil photolysis half

- life (25  C) Stable Does not photodegrade on soil. MRID 448892

- 28

Aerobic soil metabolism half

life (2 5°C) 62.4 days Including unextracte d residues (up to 62%), the t 1/2 

### = 267 days. Data are only available for a single soil. MRID 449299

- 31

Ana erobic soil metabolism half

life (25°C) No data

− −
Anaerobic aquatic metabolism half

- life (20

- 2 5  C) 88 days (silt loam) 109 days (sandy loam) Moderately persistent. MRID 453593

- 01

Aerobic aquatic metabolism half

- life (20

- 25  C) No data

− −
Mobility

Freundlich soil

- water partition coefficients (K F 

### ) ; organic carbon

- normalized Freundlich coefficients (K FOC 

### ) Adsorption 0.60 (sand, 0.2% om) 0.62 (silt loam, 0.4% om) 0.93 (sandy loam, 1% om) 2.0 (clay loam, 2.8% om) 1.6 (silt loam sediment, 1.2% om) MRID 448892

- 31

### Page 7 of 51 Parameter Value Description /Comments Source

Organic carbon

- normalized distribution coefficient (K oc; 

### mL/g) Adsorption 143 (clay loam) 186 (sandy loam) 308 (silt loam) 604 (sand) 270 (silt loam sediment) Compound is expected to be moderately mobile in most soils based on K oc 

### . However, when present as the anion, mobility would be grea ter than expected based on this parameter, as the K oc 

### model would not be appropriate. MRID 448892

- 31

Field Dissipation

Terrestrial field dissipation half

life; leaching depth ; degradates IL : t 1/2 

### = not available; 0

- 3”; DesMe

- 180 at max of 0.03 mg/kg at 0

- 3” day 14; no other degradates

VA : t 1/2 

### = not available; 0

- 3”; MeBa at max of 0.03 mg/kg at 0

- 3” days 10

- 14 only; no other degradates Minimal residues reached soil and residues in turf were not determined. Half

- lives could not be calculated. MRID 459027

- 01 MRID 459027

- 02

Aquatic field dissipation half

life LA: t 1/2 

### = 11 days AR: t 1/2 

### = 11 days FL: t 1/2 

### = 25 days In LA and AR, pesticide was applied to soil, with flooding after 1 day. In FL, applic

. was directly to water and pesticide essentially remai ned in water column. MRID 448892

- 35 MRID 449299

- 32 MRID 476353

- 04

Fish Bioconcentration

Fish bioconcentration factors (steady

- state ); depuration rate No data Not expected to bioconcentrate based on K ow 

### . *Goring et al. 1975 scale was used to describe p ersistence. Nonpersistent: t 1⁄2 

### < 15 days; slightly persistent
t 1⁄2 

### 15

- 45 days; moderately persistent t 1⁄2 

### 45

- 180 days; persistent t 1⁄2 

### >180 days.

a. Transformation Products

Nine degradates of bispyribac

- sodium were observed in submitted environmental fate studies, 7 of which were major ( e.g. , those present at greater than 10% applied radioactivity) , including unextracted residues ( Table 3) . All of the major degradates were observed in the anaerobic aquatic metabolism studi es

. DesMe

- 2023 is a single demethylation product and most similar to the bispyribac

- sodium. DesMe

- 180 is also similar having the loss of a single pyrimidine ring side

- chain. The remaining degradates are either single ring structures, carbon dioxide, or unextracted residues, and were all observed under additional conditions ( e.g ., aerobic soil, anaerobic soil, or hydrolysis in addition to anaerobic aquatic ). The degradates 2,6

- DBA and MeBa were observed at the highest percentage , 42 and 45 % of the appli ed

### Page 8 of 51

radioactivity ( AR ) , with the remaining degradates present between 10 and 25%. All of the major organic degradates will be included in the exposure assessments for ecological risk.

In a meeting on 7/31/01, the OPP Health Effects Division ( HED ) Metaboli te Assessment Review Committee (MARC) concluded that the residue of concern to humans in drinking water is bispyribac

sodium alone (Memo, J. Tyler, 8/20/01; D276560). All metabolites identified were present at low levels, and are not expected to be more to xic to humans than the parent.

### Page 9 of 51



### Table 3. Bispyribac

- sodium and Its Major Environmental Degradates in Laboratory Studies

### Page 10 of 51 Chemical Name Chemical Structure Study Type Ref. (MRID) Maximum

%AR (day) A 

### Final %AR

(study length)
PARENT

bispyribac

- sodium sod ium 2,6

- bis[(4,6

-

dimethoxy

- 2

- pyrimidinyl)oxy]benzoate (CAS)

sodium 2,6

- bis(4,6

- dimethoxypyrimidin

- 2

- yloxy)benzoate (IUPAC)

MAJOR TRANSFORMATION PRODUCTS

DesMe

- 2023 ( Des

- Methyl KIH

- 2023 Na Salt) sodium 2

- (4,6

-

dimethoxypyrimidin

- 2

- yl)oxy

- 6

- (4

- hydrox y

- 6

- methoxypyrimidin

- 2

- yl) benzoate Aerobic Soil Metabolism 449299

- 31 8.7 (14) 0.02

- 0.3 (348)

Anaerobic Aquatic Metabolism 453593

- 01 24.8 (183) 5.7

- 20.3 (367) O C O 2 N

N O

O C H 3

C H 3 N

N OH

O C H 3 O

Na +

### Page 11 of 51 Aquatic Field Dissipation (AK) 449299

- 32 7.1 ppb ( 0.0028 ppm; in sediment; day 3) 7.1 ppb

( 0.0028 ppm; in sediment; day 3) Aquatic Field Dissipation (FL) 476353

- 04 4.3 ppb (28; in water) 1.1 ppb

(120; in water)

Aquatic Field Dissipation (LA) 448892

- 35 0.0051 ppm (3,5 days; in soil)

0.00 12 ppm ( 8 ; in water ) 0.00 39 ppm (7 ;

i n soil)

0.00 12 ppm ( 8 ; in water )

Bx

- 180 2

- [(4,6

-

dimethoxypyrimidin

- 2

- yl)oxy]

- 6

- hydroxybenzoate Hydrolysis (pH 5) 448892

- 26 5.9 (30) 5.9 (30)

Aerobic Soil Metabolism 449299

- 31 11.4 (55) 1.9

- 3.4 (348)

Anaerobic Aquatic Metabolism 453593

- 01 6. 7 (275) nd (367)

DesMe

- 180 2

- hydroxy

- 6

- [(4

- hydroxy

6

- methoxypyrimidin

- 2

- yl)oxy] benzoic acid Aerobic Soil Metabolism 449299

- 31 8.1 (55) 2.5

- 3.3 (348)

Anaerobic Aquatic Metabolism 453593

- 01 15.8 (122) nd

– 13.7 (367) O C OO H N

N O

O C H 3

C H 3 O H

O C OOH
N

N OH

O C H 3 OH

### Page 12 of 51 2,6

- DBA 2,6

- dihydroxybenzoic acid Aerobic Soil Metabolism 44929

- 931 11.8 (187) 9.2 (348)

Anaerobic Aquatic Metabolism 453593

- 01 42.3 (367) 34.2

- 42.3 (367)

MeBa 4,6

- dihydroxy

- 2

-

methoxypyrimidine Aerobic Soil Metabolism 449299

- 31 7.6 (33) 1.4 (348)

Anaerobic Aquatic Met abolism 453593

- 01 44.5 (275) 18.2

- 40.9 (367)

Me 2 Ba 4,6

- dimethoxy

- 2

-

hydroxypyrimidine Hydrolysis (pH 5) 448892

- 26 12.2 (30) 12.2 (30)

Aerobic Soil Metabolism 449299

- 31 12.1 (14) 1.4 (348)

Anaerobic Aquatic Metabolism 453593

- 01 21.9 (367) 2

- 21.9 (367)

Aquatic Field Dissipation (AK) 449299

- 32 9.2 (0.0013 ppm; in water; day 3) 9.2

(0.0013 ppm; in water; day 3) OH C OOH OH

HO N

N OH

OCH 3

O H N

N OCH 3

OCH 3

### Page 13 of 51 2,6

- MDB methyl 2,6

-

dihydroxybenzoate Aerobic Soil Metabolism 449299

- 31 9.6 (257) 5.1 (348)

CO 2 carbon dioxide Aerobic Soil Metabolism 449299

- 31 57 (348) 32

- 57 (348) Anaerobic Aquatic Metabolism 453593

- 01 22.4 (367) 2.6

- 22.4 (367)

Unextracted Residues 

### ̶ Aerobic Soil Metabolism 449299

- 31 28.4

- 55 (257) 26.3

- 54.3 (348) Anaerobic Aquatic Metabolism 453593

- 01 21.8 (367) 9.7

- 21.8 (367)

n.d. means “not detected” Ranges are used when more than one label and/or more than one soil/sediment were studied.

A P ercent of Applied Radioactivity OH C O 2 CH 3 OH

### Page 14 of 51

6. Receptors

Table 4 and Table 5 provide a summary of the most sensitive acute and chronic toxicity values for bispyribac

- sodium across aquatic and terrestrial taxonomic groups, res pectively. Based on the available acute toxicity data, aquatic and terrestrial plants appear to be the most sensitive taxa, which is expected for an herbicide. B ispyribac

- sodium is practically nontoxic to the remaining taxa evaluated including freshwater and estuarine/marine fish and aquatic invertebrates, birds, mammals, and terrestrial insects. F reshwater fish are used as surrogate for aquatic

- phase amphibians and birds are used as surrogates for terrestrial

- phase amphibians and reptiles.

a. Effects to A quatic Organisms

Table 4 . Summary of the Most Sensitive Endpoints from Aquatic Toxicity Studies for B ispyribac

- Sodium

.

Taxonomic Group Study

Type Test Species Toxicity Value Acute Toxicity Classificati

on Source

(MRID)

Freshwater fish 1 Acute Rainbow t rou t Oncorhynchus mykiss LC 50 > 102 mg a.i./L

[TGAI (95.2% a.i.)] Practically nontoxic 448891

- 14

Bluegill sunfish Lepomis macrochiru s LC 50 > 102 mg a.i./L

[TGAI (95.2% a.i.)] Practically nontoxic 448891

- 15

Chronic Fathead minnow

Pimephales promelas NOA EC = 9.2 mg a.i./L

[TGAI (99.6%)] -- 481142

- 01

Freshwater invertebrates Acute Waterflea Daphnia magna EC 50 > 99.2 mg a.i./L [TGAI (95.2%)] Practically nontoxic 448891

- 16

Chronic Waterflea

Daphnia magna EC 50 >99.2 mg a.i./L [TGAI (95.2%)] -- 448891

- 20

Estuarine/marine fish Acute Sheepshead minnow Cyprinodon variegatus LC 50 > 120 mg a.i. /L

[Bispyribac

- sodium 80 S (79% a.i.)] Practically

nontoxic 448891

- 18

Chronic (none)

-- -- --

Estuarine/marine shrimp Acute Mysid shrimp Americamysis bahia EC 50 >130 mg a.i./L

[Bispyribac

- sodium 80 S (79% a.i.)] Practically

nontoxic 448891

- 19

### Page 15 of 51 Taxonomic Group Study

Type Test Species Toxicity Value Acute Toxicity Classificati

on Source

(MRID)

Estuarine/marine mollusk Acute Eastern oyster Crassostrea virginica EC 50 >110 mg a.i./L

[Bispyribac

- sodium 80 S (79% a.i.)] Practically

nontoxic 448891

- 17

Aquatic plants and algae Vascular Duckweed Lemna gibba EC 50 = 0.012 mg a.i./L

based on reduced frond density and biomass NOAEC = 0.0066 mg a.i./L ; [Bispyribac

- sodium 80 S (79% a.i.)] Very

highly toxic 448892

- 23

Non

vascular Green Algae

Pseudokirchneriell a subcapitata forme rly Selenastrum capricornutum EC 50 = 0.25 mg a.i./L

NOAEC = 0.031 mg a.i./L Highly

toxic 460766

- 02

1 f reshwater fish act as surrogates for aquatic phase amphibians

### Page 16 of 51 b. Effects to Terrestrial Organisms

Table 5 . Summary of the Most Sensitive Endpoints from Terrestrial Toxicity Studies for Bispyribac

- sodium

.

### Page 17 of 51

Taxonomic Group Study Type Test Species Toxicity Value Acute Toxicity Classifica tion Source

Birds 1 

### Acute Northern bobwhite quail Colinus virginianus LD 50 > 2,250 mg/kg

- bw

[TGAI (99.9%)] 

### Practically

n ontoxic 449299

- 03

Sub

- acute dietary Northern

bobwhite quail Colinus virginianus LC 50 >5,620 mg/kg

- diet

[TGAI (99.9%)] 

### Practically

nontoxic 449299

- 05

Chronic Northern

bobwhite quail Colinus virginianus NOAEC = 1,000 mg

a.i./kg

- diet [TGAI (99.9%)] 

### - - 451973

- 02

Mammals Acute R at Rattus norvegicus LD 50 = 3,565 mg a.i./kg

bw [TGAI (95.2%)] 

### Practically

nontoxic 448891

- 27

Chronic R at

Rattus norvegicus NOAEC = 1,000 mg

a.i./kg

- diet [TGAI (98.2%)] 

### -- 449299

- 23

Invertebrates Acute Honey bee Apis me l lifer

a LD 50 > 25 μg/bee

( Acute contact ) [TGAI (99.9%)] 

### Practically

nontoxic 448892

- 24

Sub

Chronic Earthworm

Eisenia foetida LC50 > 1,000 ppm

[TGAI (95.7%)] 

### Slightly

toxic 448892

- 25

Terrestrial plants Seedling

Emergence Dicot (cabbage) EC 25 = 0.0019 lb a.i./a cre based on reduced dry weights NOAEC = 0.0004 lb a.i./acre (80% WP Formulation) 

### -- 448892

- 22

Seedling Emergence Monocot

(onion) EC 25 = 0.0013 lb

a.i./acre based on reduced dry weights NOAEC = 0.0004 lb a.i./acre (80% WP Formulation) 

### -- 448892

- 22

### Page 18 of 51 Vege tative Vigor Monocot

(onion) EC 25 = 0.0021 lb

a.i./acre based on reduced plant height NOAEC = 0.0007 lb a.i./acre (80% WP Formulation) 

### -- 448892

- 21

Dicot (cabbage) EC 25 = 0.001 lb a.i./acre

NOAEC = 0.0007 lb a.i./acre based on reduced dry weight (80% WP Formulation) 

### -- 448892

- 21

1 Birds a re surrogates for terrestrial

- phase amphibians and reptiles.



### c. Ecological Incidents

The ecological incident information system (EIIS) is an OPP database that houses ecological incidents that have been reported to the Ag ency. When available, EIIS includes date and location of an incident, type and magnitude of effects observed in various species, use(s) of pesticides known or suspected of contributing to the incident, and results of any chemical residue analysis or other analyses conducted during incident investigation. EIIS incidents are categorized according to the certainty that the incident resulted from pesticide exposure. The Avian Monitoring System (AIMS) is a database administered by the American Bird Conservanc y that contains publicly available data on reported avian incidents involving pesticides. Many of the incidents listed in this database are also in the EIIS. As of March 25, 2014, there were n o inciden ts reported for bispyribac

- sodium in either database.

7. Exposure Pathways of Concern

The environmental fate properties and use patterns of bispyribac

- sodium indicate that direct spray, spray drift, and direct application to surface water represent potential transport and/or
exposure mechanisms of bispyribac

- sodium to non

- target aquatic and terrestrial organisms. Drinking water and inhalation exposure pathways were screened using the SIP (Screening Imbibition Program) and STIR (Screening Tool for Inhalation Risk) screening m odels . STIR indicated the inhalati on pathway is not likely to be a significant pathway for exposure for birds and mammals

. Screening

- level calculations using SIP have suggested that drinking water exposure alone may be significant for acute and chronic risks to birds and mammals ( Appendix C ). However, as indicted in Table 5 , bispyribac

- sodium is practically nontoxic to birds and mammals, therefore, it is the high solubility of bispyribac

- sodium (73,300 mg/L) which is likely driving this concern. This pathway should be further evaluated d uring risk assessment

. SIP and STIR are described in detail at http://www.epa.gov/oppefed1/models/terrestrial/index.htm

.

8. Analysis Plan

a. Stressors of Concern

i. Ecological Risk Asse ssment

### Page 19 of 51

S everal degradates were identified in the bispyribac

- sodium environmental fate studies (Table 3)

. Major degradates

, those that exceed ed 10% of the AR, include DesMe

- 2023 (25%), Bx

- 180 (11%), DesMe

- 180 (16%), 2,6

- DBA (42%), MeBa (45%), and Me 2 

### Ba (2 2%)

. QSAR analysis ( ECOSAR : ECOlogical Structure Activity Relationship, v1.11) indicates these degradates are likely to be more toxic than the parent for several taxa, however, plants, the tax o n of most
concern, were not included in the estimates (Appendi x D)

. Because of the uncertainty regarding the toxicity to plants, all major degradates will be included in the EECs. Any additional information on the degradates would likely reduce the uncertainty, but the current approach will adequately characterize potential risk.

ii. Drinking Water

Per discussion, HED does not currently anticipate updating the dietary exposure estimates from food, given that it is not likely to have a significant impact on aggregate risk. Additionally, EFED believes recent changes in the drinking water exposure models will not impact the previously estimated EDWCs in a way that would significantly impact the human dietary risk picture. Thus, an updated drinking water assessment will not likely be needed as part of the Registration Review process.

b. Measures of Exposure

EFED will use standard available models (identified in Table 7 ) to evaluate potential exposures to aquatic and terrestrial organisms as described at http://www.epa.gov/pesticides/science/models_db.htm

. Aquatic exposure estimates will be based on estimated environmental concentrations (EECs) generated using the Pesticide Root Zone Model (PRZM 3.12.2 dated May 12, 2005) coupled with the Exposure Ana lysis Model System (EXAMS 2.98.04.06 dated April 25, 2005) to simulate a waterbody that is considered representative of vulnerable aquatic environments ( e.g ., ponds and streams in headwater watersheds). Terrestrial exposure will be estimated using a model (T

- REX version 1.5) that assumes direct application to a variety of avian and mammalian food items. Exposure to terrestrial plants will be estimated using the terrestrial plant model TerrPlant ( version 1.2.2) that assumes bispyribac

- sodium drifts or move s with runoff to adjacent habitats. For more information on the various models that will be used in the risk assessment see the links as outlined in Table 7

. Two spray drift models, AgDISP and/or AgDRIFT, will be used to assess exposures of aquatic and
t errestrial organisms to bispyribac

- sodium deposited in terrestrial and aquatic habitats by spray drift. The AgDRIFT (version 2.1.1; dated 03/07/2012) model is most commonly used by EFED to simulate spray drift into terrestrial and aquatic environments. Th e AgDISP model (version 8.26; dated 03/07/2012) is used when a parameter needs to be modeled that is not available in AgDRIFT. Spray drift analysis will be an important part of the analysis in defining the potential area of effects for listed species.

S I P (version 1.0; dated Aug. 19, 2010) and STIR (version 1.0; dated Nov. 23, 2010) are used to calculate screening

- level estimates of toxicity via the drinking water and inhalation pathways

, respectively . The models produce toxicity estimates for mammals an d birds under acute and chronic exposure scenarios.

### Page 20 of 51

Table 7 . List of the various models and the related taxa for which the models will be used.

Environment Taxa of

Concern Model(s) Link

Aquatic Fish/

Invertebrates/ Plants PRZM, EXAMS http://www.epa.gov/oppefed1/models/water /

Terrestrial Vertebrate T

- REX (dietary item) http://www.epa.gov/oppefed1/models /terres trial/trex/trex_v1_5_webversion.xlsm

AGDISP (Spray drift) http://www.continuum

- dynamics.com/pr

agdisp.html
SIP (imbibition) 

### http://www.epa.gov/oppefed1/models/te

rrestrial/
STIR (Inhalation) 

### http://www.epa.gov/oppefed1/models/te

rrestrial/

Plant TerrPlant (Run

- off) http://www.epa.gov/oppefed1/models/terres trial/index.htm#terrplant AgDRIFT (Spray drift) http://www.agdrift.com/AgDRIFt2/Downlo

a dAgDrift2_0.htm AGDISP (Spray drift) http://www.continuum

- dynamics.com/pr

agdisp.html

i. Available Monitoring Data

The Agency is aware of monitoring conducted by federal and /or state a gencies and this information will be considered in the assessment to the extent that data on bispyribac

- sodium or other residues of concern are available.

c. Measures of Effect

The most sensitive toxicity endpoints presented in Section 6 of this problem fo rmulation will be used to calculate risk quotients for the respective taxa. Any additional information submitted by the registrant or found in the open literature prior to conduct of the risk assessment will also be considered. The open literature studie s will be identified using EPA’s ECOTOXicology database ( ECOTOX ; http://cfpub.epa.gov/ecotox/ ) , which employs a literature search engine for locating chemical toxicity data for aquatic life, terrestrial plants, and wildlife. D ata that meet the Agency’s standard for inclusion in risk assessments can also provide insight into the direct and indirect effects of pesticides on biotic communities . Such effects could include loss of species that are sensitive to the c hemicals and from changes in structure and functional characteristics of the affected communities.

9. Endangered Species Assessments

Consistent with the Agency’s responsibility under the Endangered Species Act (ESA), the Agency will evaluate risks to listed species from registered uses of pesticides in Registration Review . The process for evaluating potential risks to listed species is further described at

### Page 21 of 51

http://www.epa.gov/oppfead 1/endanger/litstatus/riskasses.htm

.

10. Endocrine Disruptor Screening Program

As required by FIFRA and FFDCA, EPA reviews numerous studies to assess potential adverse outcomes from exposure to chemicals. Collectively, these studies include acute, subchron ic and chronic toxicity, including assessments of carcinogenicity, neurotoxicity, developmental, reproductive, and general or systemic toxicity. These studies include endpoints which may be susceptible to endocrine influence, including effects on endocrine target organ histopathology, organ weights, estrus cyclicity, sexual maturation, fertility, pregnancy rates, reproductive loss, and sex ratios in offspring. For ecological hazard assessments, EPA evaluates acute tests and chronic studies that assess grow th, developmental and reproductive effects in different taxonomic groups. As part of i ts reregistration decision

, EPA reviewed these data and selected the most sensitive endpoints for relevant risk assessment scenarios from the existing hazard database. However, as required by FFDCA section 408(p), bispyribac

- sodium is subject to the endocrine screening part of the Endocrine Disruptor Screening Program (EDSP).

EPA has developed the EDSP to determine whether certain substances (including pesticide active and other ingredients) may have an effect in humans or wildlife similar to an effect produced by a “naturally occurring estrogen, or other such endocrine effects as the Administrator may designate.” The EDSP employs a two

- tiered approach to making the st atutorily required determinations. Tier 1 consists of a battery of 11 screening assays to identify the potential of a chemical substance to interact with the estrogen, androgen, or thyroid (E, A, or T) hormonal systems. Chemicals that go through Tier 1 sc reening and are found to have the potential to interact with E, A, or T hormonal systems will proceed to the next stage of the EDSP where EPA will determine which, if any, of the Tier 2 tests are necessary based on the available data. Tier 2 testing is des igned to identify any adverse endocrine

- related effects caused by the substance, and establish a dose

- response relationship between the dose and the E, A, or T effect.

Under FFDCA section 408(p), the Agency must screen all pesticide chemicals. Between O ctober 2009 and February 2010, EPA issued test orders/data call

- ins for the first group of 67 chemicals, which contains 58 pesticide active ingredients and 9 inert ingredients. A second list of chemicals identified for EDSP screening was published on June 14, 2013 2 

### and includes some pesticides scheduled for registration review and chemicals found in water

. Neither of these lists should be construed as a list of known or likely endocrine disruptors. For further information on the status of the EDSP, the pol icies and procedures, the lists of chemicals, future lists, the test guidelines and the Tier 1 screening battery, please visit our website: http://www.epa.gov/endo/

.
11. Preliminary Identification of Data Gaps

a. Environm ental Fate

Table 8 identifies environmental fate studies by MRID that offer data for each guideline requirement, as well as study classifications and whether or not further data are needed in order to support risk assessment.

2 See http://www.regulations.gov/#!documentDetail;D=EPA

- HQ

- OPPT

- 2009

- 0477

- 0074 for the final second list of chemicals.

### Page 22 of 51

Table 8 . Environmental Fate Data and Study Classifications for Bispyribac

- Sodium

.

OCSPP Guideline Data

Requirement Submitted

Studies (MRID) Study

Classification Are data

needed to conduct risk assessment? Justification and

Assumptions EPA wil l Make in Absence of Data

835.2120 Hydrolysis 44889226 Acceptable No

−

835.2240 Aqueous

photolysis 44889227 Acceptable No

−

835.2410 Soil photolysis 44889228 Acceptable No

−

835.2370 Photodegradation

in Air Not required

− No

−

835.4100 Aerobic soi l

metabolism 44929931 Acceptable Yes. Need data for 3

more soils

. The single available half

- life

will be multiplied by 3 to account for uncertainty in using a single value.

835.4200 Anaerobic soil

metabolism No data

− No Acceptable anaerobic aquatic metab olism data will be used.

835.4300 Aerobic aquatic

metabolism 44889230 Downgraded to Not A cceptable Yes The half

- life input will be estimated using 2X the aerobic soil half

- life input value.

835.4400 Anaerobic

aquatic metabolism 45359301 Acceptable No

−

835.1230 835.1240 Adsorption/

desorption and leaching 44889231 Acceptable No

−

835.1410 Volatility

–

laboratory Not required

− No

−

835.6100 Terrestrial field

dissipation 45902701 Supplemental No

−

45902702 Supplemental

835.6 2 00 Aquatic field

dissi pation 44889235 Acceptable

No

−

44929932 Acceptable 47635304 Acceptable 44889232 Not Acceptable

### Page 23 of 51

OCSPP Guideline Data

Requirement Submitted

Studies (MRID) Study

Classification Are data

needed to conduct risk assessment? Justification and

Assumptions EPA wil l Make in Absence of Data

850.6100 Analytical

method in soil 45243501

452435 02 (Me 2 Ba)

45243503 ( MeBa, Des Me

- 2023 ) Acceptable

Acceptable

Acceptable 

### No

−

Analyti cal method in water 45243503

( MeBa, Des Me

- 2023 ) Acceptable


### No

−

850.1730 Fish

bioconcentration Waived

− No

−

b. Ecological Effects

Da ta for passerine species of birds are required under the new 40 CFR Part 158 (Oct. 26, 2007). Part 158 data requirem ents specify that avian acute oral toxicity data (OCSPP Guideline 850.21 00) be submitted for either a mallard duck (Anas platyrhynchos) or bobwhite quail (Colinus virginianus) and a passerine species. Based on existing data, bispyribac

- sodium is characteri zed as practically non

- toxic to birds on an acute oral (bobwhite quail LD 50 

### >2250 mg/kg bw) and subacute dietary (bobwhite quail and mallard duck LC 50 

### >5620 mg/kg diet) exposure basis. According to the Avian Incident Monitoring System (AIMS) and the EPA Eco logical Incident Information System (E II S) there have been no incidents involving avian species or other taxa associated with the use of bispyribac

- sodium. Additionally, prior risk assessments have not identified any risk to avian species, and we do not ex pect this risk conclusion to change for currently proposed uses

. Therefore, we are not requesting these data at this time

.

A h oney bee larval toxicity test (OECD 237) is being required at this time to assess the toxicity of bispyribac

- sodium to developing bee larva

. Although a number of studies were conducted with technical end

- use product ( TEP ) instead of technical grade active ingredient ( TGAI ) as required by the regulatory guidelines, a thorough review by EFED indicated that the percentage of TGAI is su fficiently high that new studies using TGAI are not required ( USEPA, 2010c).

Table 9 and 10 identify ecological effects studies by MRID that provide data for each guideline requirement, as well as study classifica tions and whether or not further data are needed in order to support risk assessment. Additional r ationale for the required studies is also presented

.

### Page 24 of 51

Table 9 . Aquatic Ecological Effects Data and Study Classifications for Bispyribac

- S odium.

OCSPP Guidel ine Data

Requirement Study

Type Submitted

Studies (MRID) Study

Classification Are data

needed to conduct risk assessment Justification and

Assumptions EPA will Make in Absence of Data

- Some Example texts are include

850.1010 Freshwater

invertebrate toxic ity Acute 448891

- 16 Supplemental No

--

850.1025 850.1035 850.1045 850.1055 Saltwater

invertebrate toxicity Acute 448891

- 17 Supplemental

No

-- 448891

- 19 Supplemental

850.1075 Freshwater fish Acute 448891

- 14 Supplemental No

-- 448891

- 15 Supplemen tal

850.1075 Saltwater fish Acute 448891

- 18 Acceptable No

--

850.1300 Freshwater

invertebrates life cycle Chronic 448891

- 20 Acceptable No

--

850.1350 Saltwater

invertebrates life cycle Chronic (None) ( N / A ) No

--

850.1400 Freshwater fish

early

- life st age Chronic 481142

- 01 Acceptable No

--

850.1400 Saltwater fish

early

- life stage Chronic NA NA No

--

850.1500 Freshwater fish

full life cycle Chronic (None) (N/A) No

--

850.1735 850.1740 10

- day

Sediment Toxicity Test Subacute (None) (NA) No

850.4400 Aq uatic plant

Toxicity Test using Lemna minor

. -- 448892

- 23 Acceptable No

--

850.4500 Algal toxicity -- 460766

- 02 Acceptable

No

-- -- 448892

- 17 Supplemental

-- 448892

- 19 Acceptable

-- 448892

- 20 Acceptable

850.4550 Cyanobacteria 448892

- 18 Ac ceptable No

--

### Page 25 of 51

Table 10. Terrestrial Ecological Effects Data for Bispyribac

- S odium

.

OCSPP Guideline Data

Requirement Study

Type Submitted

Studies (MRID) Study

Classifications Are data

needed for risk assessment Current Additional Data Need

850.2100 Avi an oral

toxicity Acute 449299

- 03 Acceptable No

--

Passerine Acute (None) ( N / A ) No Acute avian oral toxicity data are required for either one waterfowl or one upland game species and one passerine species under the 40 CFR Part 158 (2010) data requirements for conventional pesticides with outdoor uses. However, there were no mortalities in acute or subchronic dietary studies including bobwhite quail and mallard duck and it is likely that results of a passerine species will be similar. Therefore, this stud y is not required at this time.

850.2200 Avian dietary

toxicity Subacute 449299

- 04 Acceptable

No

-- 449299

- 05 Acceptable

850.2300 Avian

reproduction Chronic 451973

- 01 Acceptable
No

-- 451973

- 02 Acceptable

Non

guideline Avian

inhalation Acute (None) ( N / A ) No Bispyribac

- sodium has a vapor point of 1 x10

- 7 Torr and is not expected to volatilize. EFED’s STIR model further indicated that inhalation is not likely to be a significant pathway for exposure. Therefore, this study is not required at

t his time.

850.3020 Honey bee

acute contact toxicity Acute 448892

- 24 Acceptable No

--

Non

Guideline Honey bee

larval toxicity test (OECD 237) Acute (None) (NA) Yes This study is being required to assess the toxicity of bispyribac

sodium to developing bee

larva

### Page 26 of 51 OCSPP Guideline Data

Requirement Study

Type Submitted

Studies (MRID) Study

Classifications Are data

needed for risk assessment Current Additional Data Need

Non

Guideline Earthworm

toxicity test Acute 448892

- 25 Supplemental No

--

850.3030 Honey bee

residue on foliage -- (None) ( N / A ) No The LD 50 for the honey bee acute contact study was > 25 μg a.i./bee, above the trigger for the honey bee residue on fo liage study of 11 μg/bee. Furthermore, bispyribac

- sodium has not been highlighted as a pervasive pesticide in bee colonies and oral exposure will be negligible based on the use pattern. Therefore, this study is not being required at this time.

850.3040 Field testing

for pollinators -- (None) ( N / A ) No See explanation for Honey bee residue on foliage.

850.4100 Seedling

Emergence and Seedling Growth -- 448892

- 22 Acceptable No

--

850.4250 Vegetative

Vigor -- 448892

- 21 Acceptable No

--



### 12. References

Food a nd Agriculture Organization of the United Nations. 2000. FAO PESTICIDE DISPOSAL SERIES 8. Assessing Soil Contamination: A Reference Manual. Appendix 2. Parameters of pesticides that influence processes in the soil. Editorial Group, FAO Information Divisio n. Rome.

Goring, C.A.I., D.A. Laskowki, J.H. Hamaker, and R.W. Meikle. 1975. Principles of pesticide degradation in soil. Pages 135

- 172 in (R. Haque and V.H. Freed, eds.) Environmental dynamics of pesticides . Plenum Press, New York.

USEPA. 2002a. Guidanc e for Selecting Input Parameters in Modeling the Environmental Fate and Transport of Pesticides, Version II (February 28, 2002). Office of Pesticide Programs, Environmental Fate and Effects Division, U.S. Environmental Protection Agency. Arlington, VA.

U SEPA . 2002b. Pesticide Root Zone Model (PRZM) Field and Orchard Crop Scenarios: Standard Procedures for Conducting Quality Control and Quality Assurance. Office of Pesticide Programs, Environmental Fate and Effects Division, U.S. Environmental Protection A gency. Arlington, VA.

### Page 27 of 51

USEPA. 2004a. 

### Risk Assessment for Proposed Use of Bispyribac

- Sodium (Velocity ® ) on Turf

. DP Barcode D305493

. United States Environmental Protection Agency (USEPA). Environmental Fate and Effects Division. Office of Pesticide Progra ms. August 5 , 2004.

USEPA. 2004b. Overview of the Ecological Risk Assessment Process in the Office of Pesticide Programs . United States Environmental Protection Agency (USEPA). Environmental Fate and Effects Division. Office of Pesticide Programs. Availa ble at http://www.epa.gov/espp/consultation/ecorisk

- overview.pdf

.

USEPA. 2010a. Ecological Risk Assessment for the Bispyribac Sodium Section 3 New Use as an Aquatic Herbicide 

### . DP Barcode D36 1120

. United States Environmental Protection Agency (USEPA). E nv ironmental Fate and Effects Division. Office of Pesticide Programs. October 26 , 2010.

USEPA. 2010 b

. 

### Revised Tier I Drinking Water Assessment in Support of the Proposed Section 3 New Use Registration for Bispyribac Sodium for Aquatic Use . DP Barcode D362 285

. United States Environmental Protection Agency (USEPA). E nvironmental Fate and Effects Division. Office of Pesticide Programs. May 20, 2010.

USEPA. 2010c. EFED Review of “ 

### Waiver Request: Toxicity of Bispyribac

- Sodium Typical End

- Use Product to an Aqu atic Plant ( Lemna spp.)” (MRID: 480606

- 04); and “Waiver request: Freshwater Fish and Invertebrate Acute Toxicity to Bispyribac

Sodium Typical End

- Use Product” (MRID: 480606

- 03). DP Barcode D3 77610

. United States Environmental Protection Agency (USEPA). E nv ironmental Fate and Effects Division. Office of Pesticide Programs. June 4, 2010.

USEPA. 2014

. BEAD Chemical Profile for Registration Review: Bispyribac

- sodium (078906)

. Memorandum From M. Kaul, C

. Doucoure , and A. Lee to Moana Appleyard

. Biological

an d Economic Analysis Division to Pesticide Re

- evaluation Division. March 12 , 201 4

.

USEPA. 2013. Bispyribac

- sodium (078906) Screening Level Usage Analysis (SLUA)

. United States Environmental Protection Agency (USEPA). Biological and Economic Analysis Div ision , Office of Pesticide Programs. April 22, 2013

.

### Page 28 of 51

Appendix A

. Bibliography of Submitted Studies

ENVIRONMENTAL FATE

44889226 2017179 Velagaleti, R.; Williamson, K.; Williams, M. (1998) Hydrolysis of KIH

2023 as a Function of pH at 25 (degrees) C: Lab Project Number: 39267: 9900030: N

- 161

- 1. Unpublished study prepared by ABC Laboratories, Inc. 157 p.

44889227 2017180 Velagaleti, R.; Williamson, K.; Williams, M. (1998) Determination of the Aqueous Photolysis Rate of KIH

- 2023: Lab Project Number: 39268 : 9900031: N

- 161

- 2. Unpublished study prepared by ABC Laboratories, Inc. 107 p.

44889228 2017181 Reynolds, J. (1999) Photolysis of (carbon

- 14) Bispyribac

- Sodium on Soil: Lab Project Number: RPT00461: 9900255: XBL98082. Unpublished study prepared by Xenob iotic Laboratories, Inc. 158 p.

44929931 2017182 Assaf, N. (1999) Metabolism of (Ph

- (carbon

- 14)) Bispyribac

- sodium and (Py

- (carbon

- 14)) Bispyribac

- sodium in Aerobic Soil: Lab Project Number: VP

- 12114: 9900243: V

- 98

- 12114. Unpublished study prepared by Va lent U.S.A. Corporation. 154 p.

44889230 2017184 Velagaleti, Ranga. November 3, 1998. Aerobic Aquatic Metabolism of 14C

- KIH

- 2023. Report Number: 39637. Unpublished study performed by ABC Laboratories, Inc., 7200 East ABC Lane, P.O. Box 1097, Columbia, Mi ssouri 65205; and submitted by Valent USA Co., Walnut Creek, California

44889229 2017185 Velagaleti, R.; Gorman, M.; Adkins, T. (1998) Anaerobic Aquatic Metabolism of (carbon

- 14)

- KIH

- 2023: Lab Project Number: 39638: 9900049: N162

- 3. Unpublished study prep ared by ABC Laboratories, Inc. 292 p.

45359301 2017189

Same Project number different citations Heim, D.; Gohre, K.; Rose, A. (2001) Anaerobic Aquatic Metabolism

of (carbon 14)

- KIH

- 2023: Lab Project Number: 39638 : 200100044. Unpublished study prepared by ABC Labs., Inc. 553 p.

45359301 2017189



# **** Velagaleti, Ranga. October 16, 1998. Anaerobic Aquatic Metabolism

of 14C

- KIH

- 2023. Report Number: 39638 . Unpublished study performed by ABC Laboratories, Inc., 7200 East ABC Lane, P.O. Box 1097, Columbia, Miss ouri 65205; and submitted by Valent USA Co., Walnut Creek, California.

4889231 2017183 Velagaleti, R.; Laster, W.; McGowan, N. (1991) Soil/Sediment Adsorption

- Desorption of (carbon

- 14)

- KIH

- 2023: Lab Project Number: 39165: 9800433: N

- 163

- 1. Unpublished stu dy prepared by ABC Laboratories, Inc. 112 p.

45902701 2016930

2016929

- memo Schreier, T. (2003) Terrestrial Field Soil Dissipation of Bispyribac

Sodium on Turf in Illinois: Lab Project Number: 23780: VP

- 23780: V

02

- 23780. Unpublished study prepared by Va lent U.S.A. Corporation. 399 p.

### Page 29 of 51

45902702 2016931

2016929

- memo Schreier, T. (2003) Terrestrial Field Soil Dissipation of Bispyribac

Sodium on Turf in Virginia: Lab Project Number: VP

- 23798: V

23798

- 02

- A: RM

- 35S4. Unpublished study prepared by Valent U.S. A. Corporation. 402 p.

44889232 2017188 Schreier, T. (1999) Aquatic Dissipation of Bispyribac

- Sodium Following Application of Regiment 80 S to a Flooded Rice Field: Lab Project Number: VP

- 11750: 9900253: V

- 97

- 11750. Unpublished study prepared by Valent U SA Corporation. 480 p.

44889233 2085193

-

upgraded Judy, D.; Lochhaas, C. (1993) Aquatic Field Dissipation Study for

KIH

- 2023, Arkansas Site: Lab Project Number: 394711: 9800314: FS

- 900501. Unpublished study prepared by ABC Laboratories, Inc. 162 p.

448 89234 2085192

-

upgrade Judy, D.; Lochhaas, C. (1994) Aquatic Field Dissipation Study for

KIH

- 2023, Louisiana Site: Lab Project Number: 394721: 9800315: FS

- 900502. Unpublished study prepared by ABC Laboratories, Inc. 163 p.

44889235 2085192

-

upgrade Judy, D.; Lochhaas, C. (1993) Aquatic Field Dissipation Study for

KIH

- 2023, Louisiana Site: Lab Project Number: 39472: 9800317: FS

900502. Unpublished study prepared by ABC Laboratories, Inc. 195 p.

44889236 2085928 Rice, F.; Judy, D.; Lochhaas, C. (1992) Com bined Magnitude of the Residue in Rice Raw Agricultural Commodities and Preliminary Aquatic Dissipation Study for KIH

- 2023: Lab Project Number: 38709: 9900050: FS

- 901510. Unpublished study prepared by ABC Laboratories, Inc. 206 p.

44929932 2085193

-

upgra de Judy, D.; Lochhaas, C. (1993) Aquatic Field Dissipation for KIH

2023, Arkansas Site: Lab Project Number: 39471: 9800316. Unpublished study prepared by ABC Laboratories, Inc. 207 p.

45243501 Supplement

1 to MRID #44889232 Schreier, T. (2000) Supplement 1 to MRID #44889232 Determination

of V

- 10029 in Soil and Soil Sediment, RM

- 35S(Bispyribac

- sodium Technical): Lab Project Number: RM

- 35S. Unpublished study prepared by Valent U.S.A. Corporation. 36 p. {OPPTS 850.7100}

D270077 2075906 Bispyribac Sodium La b Method Evaluation Report No. ECM 0183~11

- RM

- 35s: Determination of V

- 10029 in Soil and Soil Sediment

- BEAD Lab report

45243502 Supplement

1 to MRID #44889233 Schreier, T. (2000) Supplement 1 to MRID #44889233 Liquid

Chromatographic Method for Determinati on of Me2BA in Arkansas Soil (Bispyribac

- Sodium Technical): Lab Project Number: 39471. Unpublished study prepared by ABC Laboratories, Inc. 25 p. {OPPTS 850.7100}

ECM 0186S1

- 2 2063259

2063260 Environmental Chemistry Method Validation Report Number ECM

01 86S1

- 2 Bispyribac Sodium Metabolites: MeBA and DesMe

- 2023 in Soil

45243503 Supplement

2 to MRID #44889233 Schreier, T. (2000) Supplement 2 to MRID #44889233 Determination

of MeBA and DesMe

- 2023 in Water, RM

- 35WM (Bispyribac

- Sodium Technical): Lab Project Number: RM

- 35WM. Unpublished study prepared by Valent U.S.A. Corporation. 26 p. {OPPTS 850.7100}

### Page 30 of 51

ECM0186 W1

- 2 2063261 Environmental Chemistry Method Validation Report Number ECM 0186W1

- 2 Bispyribac Sodium Metabolites: MeBA and Des Me

- 2023 in Surface Water

45243504 Supplement

2 to MRID #44889233 Schreier, T. (2000) Supplement 2 to MRID #44889233 Liquid

Chromatographic Method for Determination of Me2BA in Water (Bispyribac

- Sodium Technical): Lab Project Number: 39471. Unpublished study prepared by ABC Labor atories, Inc. 24 p. {OPPTS 850.7100}

45243505 Supplement

3 to MRID #44889232 Schreier, T. (2000) Supplement 3 to MRID #44889232 Determination

of V

- 10029 in Water, RM

- 35WM (Bispyribac

- Sodium Technical): Lab Project Number: RM

- 35W. Unpublished study prepar ed by Valent U.S.A. Corporation. 35 p. {OPPTS 850.7100}

45243506 Supplement

4 to MRID #44889232 Schreier, T. (2000) Supplement 4 to MRID #44889232 Determination

of MeBA and DesMe

- 2023 in Soil, RM

- 35SM

- 1 (Bispyribac

- Sodium Technical): Lab Project Number: RM

- 35SM

- 1. Unpublished study prepared by Valent U.S.A. Corporation. 48 p. {OPPTS 850.7100}

48026101 2085270

memo re Valent Response Charlton, R. (2010) Valent Response to Data Evaluation Reports for

Aquatic Dissipation and Residue Studies for Bispyribac

- Sodium. Project Number: VP/37275, 201000091. Unpublished study prepared by Valent U.S.A. Corporation. 53 p.

45330101 2085192

-

upgraded Judy, D.; Lochhaas, C. (2001) Aquatic Field Dissipation Study for

KIH

- 2023, Louisiana Site: Lab Project Number: 394721. Unpublished study prepared by ABC Laboratories, Inc. 165 p.

45354501 2085193

-

upgraded 2085928 Pg.34 Judy, D.; Lochhaas, C. (1993) Aquatic Field Dissipation Study for

KIH

- 2023, Arkansas Site: Lab Project Number: 394711: 9800314. Unpublished study prepare d by ABC Labs., Inc. 162 p.

48026102 2085928

Pg.34 Judy, D. (2001) Aquatic Field Dissipation Study for KIH

- 2023,

Arkansas Site: Final Report. Project Number: 394712, 200200166, 394712/10. Unpublished study prepared by ABC Laboratories, Inc. 39 p.

48026 103 2085192

-

supplementa l data Judy, D. (2001) Aquatic Field Dissipation Study for KIH

- 2023,

Lousiana Site: Final Report. Project Number: 394722, 394722/3, 200200137. Unpublished study prepared by ABC Laboratories, Inc. 41 p.

47635304 2085194 White, S.M . and Barker, W. 2008. Aquatic dissipation of bispyribac

sodium in Florida during 2007. Unpublished study performed by Eurofins

- Agrisearch Americas Inc. Mebane,North Carolina (test facility), Research Options, Inc., Montezuma, Georgia (fieldphase) and En

- Ca s Analytical Laboratories, Winston

- Salem, North Carolina (analytical phase)and submitted by Valent U.S.A. Corporation, Walnut Creek, California (pp. 1, 5 and 6).Laboratory Project ID: Valent Study No. AU/11929/VL. Experiment initiation July 51, 2007 (test application; p. 15) and completion March 4,2008 (analytical phase; Appendix 3, p. 85). Finalreport issued December 19,2008. Page 3

44889006 Hewitt, A. (1999) Aerial Atomization Droplet Size Spectra for Rice Herbicides: Lab Project Number: V99

- 001: 9900236 . Unpublished

### Page 31 of 51 study prepared by Stewart Agricultural Research Services, Inc. 157 p.

47635300 Petition Valent U.S.A Corporation (2008) Submission of Residue Data in Support of the Petition for Tolerance of Bispyribac

- Sodium and its Metabolite Des

- Methyl K IH

- 2023 for Use on Fish, the Application for Registration of Tradewind Herbicide and the Amended Registration of Bispyribac

- Sodium Technical. Transmittal of 4 Studies.

47635302 ECM

Method review not located Smith, C. (2008) Analytical Method Validation fo r the Determination of

Bispyribac

- Sodium and Des

- Methyl KIH

- 2023 (Bispyribac

- Na Metabolite) Fish Tissue and in Tank Water. Project Number: 080288, 200800354. Unpublished study prepared by Golden Pacific Laboratories, LLC (GPL). 85 p.

D339872 2063653 Revie w of proposed aquatic field dissipation study protocol for bispyribac

- sodium



### ECOLOGICAL EFFECTS

44929903 2054068 Campbell, S.; Grimes, J.; Jaber, M. (1993) KIH

- 2023: An Acute Oral Toxicity Study with the Northern Bobwhite: Lab Project Number: 267

- 108 : 9900069. Unpublished study prepared by Wildlife International, Ltd. 21 p.

44929904 2054069 Campbell, S.; Beavers, J. (1993) KIH

- 2023: A Dietary LC50 Study with the Mallard: Lab Project Number: 267

- 110: 9900070. Unpublished study prepared by Wildlife In ternational, Ltd. 38 p.

44929905 2054070 Campbell, S.; Beavers, J. (1993) KIH

- 2023: A Dietary LC50 Study with the Northern Bobwhite: Lab Project Number: 267

- 109: 9900085. Unpublished study prepared by Wildlife International, Ltd. 36 p.

45197301 2054071 Frey, L.; Martin, K.; Beavers, J.; et al. (2000) Bispyribac

- Sodium: A Reproduction Study with the Mallard: Lab Project Number: 263

- 144. Unpublished study prepared by Wildlife International, Ltd. 158 p. {OPPTS 850.2300}

45197302 2054072 Frey, L.; Martin, K.; Beavers, J.; et al. (2000) Bispyribac

- Sodium: A Reproduction Study with the Northern Bobwhite: Lab Project Number: 263

- 143. Unpublished study prepared by Wildlife International, Ltd. 159 p. {OPPTS 850.2300}

44889114 2054054 Douglas, M.; Stonehewer, R.; Macdonald, I. (1998) The Acute Toxicity of KIH

- 2023 to Rainbow Trout (Oncorhynchus mykiss): Lab Project Number: KCI 45(C)/901717: 9900078. Unpublished study prepared by Huntingdon Life Sciences Ltd. 23 p.

44889115 2054055 Douglas, M.; Stonehewer, R.; Macdonald, I. (1998) The Acute Toxicity of KIH

- 2023 to Bluegill Sunfish (Lepomis macrochirus): Lab Project Number: KCI45(D)/901718: 9900079. Unpublished study prepared by Huntingdon Life Sciences Ltd. 22 p.

48060603 Waiver

request Rose, A. (2010) Waiver Request: Freshwater Fish and Invertebrate

Acute Toxicity to Bispyribac

- Sodium Typical End

- Use Product. Project Number: VP/37483, 201000122. Unpublished study prepared by Valent U.S.A. Corporation. 4 p.

### Page 32 of 51

44889116 2054056 Douglas, M.; Stonehewer, R.; Macdon ald, I. (1998) The Acute Toxicity of KIH

- 2023 to Daphnia magna: Lab Project Number: KCI45(B)/901716: 9900077. Unpublished study prepared by Huntingdon Life Sciences Ltd. 22 p.

48060603 Waiver

request Rose, A. (2010) Waiver Request: Freshwater Fish and In vertebrate

Acute Toxicity to Bispyribac

- Sodium Typical End

- Use Product. Project Number: VP/37483, 201000122. Unpublished study prepared by Valent U.S.A. Corporation. 4 p.

44889117 2083159 Dionne, E. (1998) Bispyribac

- Sodium 80S

-- Acute Toxicity to Eastern Oyster (Crassostrea virginica) Under Flow

- Through Conditions: Lab Project Number: 98

- 1

- 7229: 9900052. Unpublished study prepared by Springborn Laboratories, Inc. 73 p.

44889118 2054057 Sousa, J. (1998) Bispyribac

- Sodium 80S

-- Acute Toxicity to Sheepshead Minnow (Cyprinodon variegatus) Under Flow

- Through Conditions: Lab Project Number: 9800361: 98

- 5

- 7333. Unpublished study prepared by Springborn Laboratories, Inc. 72 p.

44889119 2054058 Putt, A. (1998) Bispyribac

- Sodium 80S

-- Acute Toxicity to Mysids (Mysi dopsis bahia) Under Flow

- Through Conditions: Lab Project Number: 98

- 3

- 7270: 9800360. Unpublished study prepared by Springborn Laboratories, Inc. 74 p.

44889120 2054059 Putt, A. (1999) Bispyribac

- Sodium

-- Full Life Cycle Toxicity Test with Water Fleas, Dap hnia magna Under Flow

- Through Conditions: Lab Project Number: VP

- 1200: 9900199. Unpublished study prepared by Springborn Laboratories, Inc. 67 p. {OPPTS 850.1300}

48114201 2085780

2085804 2085795 and 2085803=

memos Dorgerloh, M. (2001) Bispyribac

- Sodium (KIH 2023): Early Life

- Stage

Limit Test with Fathead Minnow (Pimephales promelas) under Flow

Through Conditions: Final Report. Project Number: 1022/010/122. Unpublished study prepared by Springborn Laboratories (Europe) Ag. 54 p.

44889221 2054063 R. S. Chetram, (March 4, 1998) Tier 2 Vegetative Vigor Nontarget Phytotoxicity Study using an 80% WP Formulation of V

- 10029. Valent U.S.A. Corporation Dublin Laboratory, ABC Laboratories California



### 44889222 2054064 R. Chetram, (August 4, 1998) Tier 2 Seedlin g Emergence Nontarget Phytotoxicity Study Using an 80% WP Formulation of V

- 10029 Valent U.S.A. Corporation, Dublin Laboratory ABC Laboratories California.

44889217 2054060 Douglas, M.; Stonehewer, R.; Macdonald, I. (1998) The Algistatic Activity of KIH

- 20 23: Lab Project Number: KCI 45A/901715: 9900076. Unpublished study prepared by Huntingdon Life Sciences Ltd. 26 p.

44889218 2083141

-

signed copy 2085458 Hoberg, J. (1998) Bispyribac

- Sodium

-- Toxicity to the Freshwater Blue

Green Alga, Anabaena flos

- aquae: Lab Project Number: 98

- 3

- 7263: 9800359. Unpublished study prepared by Springborn Laboratories, Inc. 65 p.

44889219 2054061 Hoberg, J. (1998) Bispyribac

- Sodium

-- Toxicity to the Freshwater Diatom, Navicula pelliculosa: Lab Project Number: 98

- 1

- 7226: 98003 70. Unpublished study prepared by Springborn Laboratories, Inc. 64 p.

### Page 33 of 51

44889220 2054062 Hoberg, J. (1998) Bispyribac

- Sodium

-- Toxicity to the Marine Diatom, Skeletonema costatum: Lab Project Number: 98

- 2

- 7251: 9800371. Unpublished study prepared by Springb orn Laboratories, Inc. 66 p.

44889223 2054065 Hoberg, J. (1998) Bispyribac

- Sodium

-- Toxicity to the Duckweed, Lemna gibba: Lab Project Number: 98

- 3

- 7275: 9800296. Unpublished study prepared by Springborn Laboratories, Inc. 78 p.

48060604 Waiver Rose, A. (2010) Waiver Request: Toxicity of Bispyribac

- Sodium Typical End

- Use Product to an Aquatic Plant (Lemna spp.). Project Number: VP/37488, 201000123. Unpublished study prepared by Valent U.S.A. Corporation. 4 p.

46076602 2016958

2016934=

memo Sayers, L. (2 003) Bispribac

- Sodium (V

- 10029)

- Toxicity to the

Freshwater Green Alga, Pseudokirchneriella subcapitata. Project Number: 12709/6220, 200300268, VP/26067. Unpublished study prepared by Springborn Smithers Laboratories. 54 p.

47954502 Open Lit Ma, J.; Lian g, W.; Xu, L.; et al. (2000) Acute Toxicity of 33 Herbicides to the Green Alga Chlorella pyrenoidosa. Bulletin of Environmental Contamination and Toxicology 66:536

- 541.

47954503 Open Lit Ma, J. (2001) Differential Sensitivity to 30 Herbicides Among Popula tions of Two Green Algae Scenedesmus obliquus and Chlorella pyrenoidosa. Bulletin of Environmental Contamination and Toxicology 68:275

- 281.

47954504 Open Lit Ma, J.; Xu, L.; Wang, S.; et al. (2001) Toxicity of 40 Herbicides to the Green Alga Chlorella vulgaris. Exotoxicology and Environmental Safety 51:128

- 132.

47954505 Open Lit Ma, J.; Lin, F.; Wang, S.; et al. (2004) Acute Toxicity Assessment of 20 Herbicides to the Green Alga Scenedesmus quadricauda (Turp.) Breb. Bulletin of Environmental Contaminat ion and Toxicology 72: 1164

- 1171.

44889224 2054066 Collins, M. (1999) Bispyribac

- Sodium

-- Acute Contact Toxicity Test with Honey Bees (Apis mellifera): Lab Project Number: 98

- 10

- 7523: 9900203. Unpublished study prepared by Springborn Laboratories, Inc. 54 p.

44889225 2054067 Johnson, A. (1997) KIH

- 2023 Acute Toxicity (LC50) to the Earthworm (Eisenia foetida): Lab Project Number: KCI 64/942475: 9900080. Unpublished study prepared by Huntingdon Life Sciences Ltd. 18 p.

45830104 Not sure if

compared to 07 8906 Rosser, S.; Kramer, V.; Krieger, M. (2002) Penoxsulam (XDE

- 638)

Comparative Ecological Risk Assessment Based on Use in U.S. Rice: Lab Project Number: GH

- C 5518. Unpublished study prepared by Dow AgroSciences LLC. 67 p.

N.A. 2075902 2001 Memo re Data review status

### Page 34 of 51

Appendix B. Pending and Active Registrations for Bispyribac

- Sodium



### Registration # Registration

Name Company # Company Name % of Active Ingredient

59639

- 105 REGIMENT

HERBICIDE 59639 VALENT U.S.A. CORP

. 80

59639

- 130 VELOCITY

HERBICI DE 59639 VALENT U.S.A. CORP

. 80

59639

- 136 VELOCITY SG

HERBICIDE 59639 VALENT U.S.A. CORP

. 17.6

59639

- 165 TRADEWIND

HERBICIDE 59639 VALENT U.S.A. CORP

. 80

63588

- 12 BISPYRIBAC

SODIUM TECHNICAL 63588 K

- I CHEMICAL U.S.A. INC 98

FL030002 REGIMENT

HERBICIDE 59639 VALENT U.S.A. CORP

. 80

GA020006 REGIMENT

HERBICIDE 59639 VALENT U.S.A. CORPORATIO N 80

MI030002 VELOCITY

HERBICIDE 59639 VALENT U.S.A. CORP

. 80

NC030007 REGIMENT

HERBICIDE 59639 VALENT U.S.A. CORP

. 80

SC030001 REGIMENT

HERBICIDE 59639 VALENT U.S.A . CORP

. 80

TX030014 REGIMENT

HERBICIDE 59639 VALENT U.S.A. CORP

. 80

59639

- EUP

13 59639 VALENT U.S.A. CORP

. 80

### Page 35 of 51

Appendix C. Ecological Model Results

A. SIP (version 1.0)

Input

Application and Chemical Information

Enter Chemical Name bisp yribac

- sodium
Enter Chemical Use herbicide Is the Application a Spray? (enter y or n) y If Spray What Type (enter ground or air) ground Enter Chemical Molecular Weight (g/mole) 452.4 Enter Chemical Vapor Pressure (mmHg) 1.00E

- 07 Enter App lication Rate (lb a.i./acre) 0.1

Toxicity Properties

Bird
Enter Lowest Bird Oral LD 50 (mg/kg bw) 2250 Enter Mineau Scaling Factor 1.15 Enter Tested Bird Weight (kg) 0.178
Mammal

Enter Lowest Rat Oral LD 50 (mg/kg bw) 1000

Enter Lowest Rat Inhalation LC 50 (mg/L) 3.35 Duration of Rat Inhalation Study (hrs) 4 Enter Rat Weight (kg) 0.35

Output
Results Avian (0.020 kg ) Maximum Vapor Concentration in Air at Saturation (mg/m 3 ) 2.43E

- 03
Maximum 1

- hou r Vapor Inhalation Dose (mg/kg) 3.06E

- 04
Adjusted Inhalation LD 50 4.20E+01

Ratio of Vapor Dose to Adjusted Inhalation LD 50 7.28E

- 06 Exposure not Likely Significant
Maximum Post

- treatment Spray Inhalation Dose (mg/kg) 1.06E

- 02

Ratio of Droplet I nhalation Dose to Adjusted Inhalation LD 50 2.51E

- 04 Exposure not Likely Significant

Results Mammalian (0.015 kg ) Maximum Vapor Concentration in Air at Saturation (mg/m 3 ) 2.43E

- 03
Maximum 1

- hour Vapor Inhalation Dose (mg/kg) 3.85E

- 04

Ad justed Inhalation LD 50 1.99E+02

Ratio of Vapor Dose to Adjusted Inhalation LD 50 1.93E

- 06 Exposure not Likely Significant
Maximum Post

- treatment Spray Inhalation Dose (mg/kg) 1.33E

- 02

Ratio of Droplet Inhalation Dose to Adjusted Inhalation LD 50 6. 66E

- 05 Exposure not Likely Significant

### Page 36 of 51

STIR (version 1.0)

Table 1. Inputs
Parameter Value
Chemical name bispyribac

- sodium Solubility (in water at 25 o C; mg/L) 73300

Mammalian LD 50 (mg/kg

- bw) 3565
Mammalian te st species laboratory rat Body weight (g) of "other" mammalian species

Mammalian NOAEL (mg/kg

- bw) 1000 Mammalian test species laboratory rat Body weight (g) of "other" mammalian species

Avian LD 50 (mg/kg

- bw) 2 250 Avian test species northern bobwhite quail Body weight (g) of "other" avian species Mineau scaling factor 1.15

Mallard NOAEC (mg/kg

- diet) 1000 Bobwhite quail NOAEC (mg/kg

- diet) 1000 NOAEC (mg/kg

- diet) for other bird species Body weight (g) of other avian species NOAEC (mg/kg

- diet) for 2nd other bird species Body weight (g) of 2nd other avian species

Table 2. Mammalian Results
Parameter Acute Chronic Upper bo und exposure (mg/kg

- bw) 12607.6000 12607.6000 Adjusted toxicity value (mg/kg

- bw) 2742.0574 769.1606 Ratio of exposure to toxicity 4.5979 16.3914

Conclusion Exposure through drinking water alone is a potential concern for mammals Exposure through d rinking water alone is a potential concern for mammals

Table 3. Avian Results
Parameter Acute Chronic Upper bound exposure (mg/kg

- bw) 59373.0000 59373.0000 Adjusted toxicity value (mg/kg

- bw) 1620.9664 49.6126 Ratio of exposure t o acute toxicity 36.6281 1196.7332

Conclusion Exposure through drinking water alone is a potential concern for birds Exposure through drinking water alone is a potential concern for birds

### Page 37 of 51

Appenidx D. EcoSAR estimates of physical chemical pro perties and toxicity for bispyribac

sodium and its major degradate s .



### A. EcoSAR estimates for bispyribac

- sodium properties

ECOSAR Version 1.11 Results Page

SMILES : n1c(OC)cc(OC)nc1Oc2cccc(Oc3nc(OC)cc(OC)n3)c2C(=O)O User SMILES: n1c(OC)cc(OC)nc1Oc2cccc(O c3nc(OC)cc(OC)n3)c2C(=O)[O

- ].[Na+] CHEM : KIH

- 2023 (sodium salt) CAS Num: 125401

- 92

- 5 ChemID1: MOL FOR: C19 H18 N4 O8 MOL WT : 430.38 Log Kow: 1.245 (EPISuite Kowwin v1.68 Estimate) Log Kow: (User Entered) Log Kow: (PhysProp DB exp value

- for comparison only) Melt Pt: (User Entered for Wat Sol estimate) Melt Pt: (deg C, PhysProp DB exp value for Wat Sol estimate) Wat Sol: 171.1 (mg/L, EPISuite WSKowwin v1.43 Estimate) Wat Sol: (User Enter ed) Wat Sol: (PhysProp DB exp value)

-------------------------------------- Values used to Generate ECOSAR Profile

-------------------------------------- Log Kow: 1.245 (EPISuite Kowwin v1.68 Estimate) Wat Sol: 171.1 (mg/L, EPISuit e WSKowwin v1.43 Estimate)

------------------------------------------------ Available Measured Data from ECOSAR Training Set

------------------------------------------------

No Data Available

-------------------------------------- ECOSAR v1.1 Clas s
- specific Estimations

--------------------------------------

********************************************************************

********** | Not Related to an Existing ECOSAR Class Definition | | | | Estimates provided below use the Neutral Organics QSAR equations which |

### Page 38 of 51

| represent baseline toxicity potential (minimum toxicity) assuming a simple | | non

- polar narcosis model. Without empirical d ata on structurally similar | | chemicals, it is uncertain if this substance will present significantly | | higher toxicity above baseline estimates. |

****************************************************************  

** ********

********************************************************************

******* * NOTE: METAL ([Na], [Li] or [K]) HAS BEEN REMOVED FOR PROPER ESTIMATION. * * SALTS SHOULD BE ENTERED IN ECOSAR AS THE NEUTRAL NON

- SALT FORM OF THE * * MOLEC ULE CORRESPONDING TO EITHER THE FREE BASE OR CONJUGATE ACID.

* * SEE HELP MENU FOR MORE INFORMATION

*

********************************************************************

*******

Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

=========================== ================== ======== ======

==========

-- > Acid moeity found: Predicted values multiplied b y 10

Neutral Organics

- acid : Fish 96

- hr LC50 16832.758 * Neutral Organics

- acid : Daphnid 48

- hr LC50 8914.475 * Neutral Organics

- acid : Green Algae 96

- hr EC50 4978.323 * Neutral Organ ics

- acid : Fish ChV 1515.524 * Neutral Organics

- acid : Daphnid ChV 716.114 * Neutral Organics

- acid : Green Algae ChV 1116.342 * Neutral Organics

- acid : Fis h (SW) 96

- hr LC50 21095.672 * Neutral Organics

- acid : Mysid 96

- hr LC50 26139.461 * Neutral Organics

- acid : Fish (SW) ChV 1444.353 *

### Page 39 of 51

Neutral Organics

- acid : Mysid (SW) ChV 2825.619 * Neutral Organics

- acid : Earthworm 14

- day LC50 8959.854 *

Note: * = asterisk designates: Chemical may not be soluble enough
to measure this predicted effect. If the effect level exceeds the water solubility by 10X, typically no effects at saturation (NES) are reported.

------------------------------ Class Specific LogKow Cut

- Offs

------------------------------ If the log Kow of the chemical is greater than the endpoint specific
cut

- offs presented below, then no effects at saturation are expected for those endpoints.

Neutral Organics:

---------------- Maximum LogKow: 5.0 (Fish 96

- hr LC50; Daphnid LC50, Mysid LC50) Maximum LogKow: 6.0 (Earthworm LC50) Maximum LogKow: 6.4 (Green A lgae EC50) Maximum LogKow: 8.0 (ChV)

--------------------------------------------------------------------

----------

### Page 40 of 51



### B. EcoSAR estimates DesMe

- 2023 properties

ECOSAR Version 1.11 Results Page

SMILES : O=C(c3c(Oc2nc(O)cc(OC)n2)cccc3Oc1nc(OC)cc(OC)n1 )O User SMILES: C(=O)(c1c(Oc2nc(O)cc(OC)n2)cccc1Oc1nc(OC)cc(OC)n1)O{

- }.[Na]{+} CHEM : KIH

- 2023 (sodium salt) CAS Num: 125401

- 92

- 5 ChemID1: MOL FOR: C18 H16 N4 O8 MOL WT : 416.35 Log Kow: 0.979 (EPISuite Kowwin v1.68 Estimate) Log Kow: ( User Entered) Log Kow: (PhysProp DB exp value

- for comparison only) Melt Pt: (User Entered for Wat Sol estimate) Melt Pt: (deg C, PhysProp DB exp value for Wat Sol estimate) Wat Sol: 353.2 (mg/L, EPISuite WSKowwin v1. 43 Estimate) Wat Sol: (User Entered) Wat Sol: (PhysProp DB exp value)

-------------------------------------- Values used to Generate ECOSAR Profile

-------------------------------------- Log Kow: 0.979 (EPISuite Kowwin v1.68 Estimate) Wat Sol: 353.2 (mg/L, EPISuite WSKowwin v1.43 Estimate)

------------------------------------------------ Available Measured Data from ECOSAR Training Set

------------------------------------------------

No Data Available

----------- --------------------------- ECOSAR v1.1 Class

- specific Estimations

-------------------------------------- Phenols

- acid

********************************************************************

******* * NOTE: METAL ([Na], [Li] or [K]) HAS BEEN REMOVED FOR PRO PER ESTIMATION. * * SALTS SHOULD BE ENTERED IN ECOSAR AS THE NEUTRAL NON

- SALT FORM OF THE * * MOLECULE CORRESPONDING TO EITHER THE FREE BASE OR CONJUGATE ACID.

* * SEE HELP MENU FOR MORE INFORMATION

*

### Page 41 of 51

****** **************************************************************

*******

Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

=========================== ================== ======== ======

==========

-- > Acid moeity found: Predicted values multiplied by 10

Phenols

- acid : Fish 96

- hr LC50 4315.354 * Phenols

- acid : Daphnid 48

- hr LC50 824.769 * Phenols

- acid : Green Algae 96

- hr EC50 4188.917 * Phenols

- acid : Fish ChV 375.151 * Phenols

- acid : Daphnid ChV 157.162 Phenols

- ac id : Green Algae ChV 1993.494 * Phenols

- acid : Fish (SW) 96

- hr LC50 2345.656 * Phenols

- acid : Earthworm 14

- day LC50 10110.711 * Phenols

- acid : Lemna gibba 7

- day EC50 3976.868 *

=========================== ================== ======== ======

========== Neutral Organic SAR : Fish 96

- hr LC50 2822.665 * (Baseline Toxicity) : Daphnid 48

- hr LC50 1458.590 * : Green Algae 96

- hr EC50 735.898 * : Fish ChV 246.879 : Daphnid ChV 109.429 : Green Algae ChV 156.235

Note: * = asterisk designates: Chemical may not be soluble enough
to measure this predicted effect. If the effect level exceeds the water solubility by 10X, typically no effects at saturation (NES) are reported.

### Page 42 of 51

------------------------------ Class Specific LogKow Cut

- Offs

------------------------------ If the log Kow of the chemical is greater than the endpoint specific
cut

- offs presented be low, then no effects at saturation are expected for those endpoints.

Phenols:

------- Maximum LogKow: 7.0 (Fish 96

- hr LC50, Daphnid LC50) Maximum LogKow: 6.4 (Earthworm, Lemna) Maximum LogKow: 7.0 (Green Algae EC50 Maximum LogKow: 8.0 (ChV) Maximum LogKo w: 5.0 (Fish (SW) 96

- hr LC50, Mysid)

Baseline Toxicity SAR Limitations:

--------------------------------- Maximum LogKow: 5.0 (Fish 96

- hr LC50; Daphnid LC50) Maximum LogKow: 6.4 (Green Algae EC50) Maximum LogKow: 8.0 (ChV)

--------------------------- -----------------------------------------

----------



### C. 

### EcoSAR estimates DesMe

- 180 properties ECOSAR Version 1.11 Results Page

SMILES : O=C(c2c(O)cccc2Oc1nc(O)cc(OC)n1)O User SMILES: C(=O)(c1c(O)cccc1Oc1nc(O)cc(OC)n1)O{

- }.[Na]{+} CHEM : DesMe

- 180 CAS Num: ChemID1: MOL FOR: C12 H10 N2 O6 MOL WT : 278.22 Log Kow: 1.457 (EPISuite Kowwin v1.68 Estimate) Log Kow: (User Entered) Log Kow: (PhysProp DB exp value

- for comparison only) Melt Pt: (User Entered for Wat Sol estimate) Melt Pt: (deg C, PhysProp DB exp value for Wat Sol estimate) Wat Sol: 3556 (mg/L, EPISuite WSKowwin v1.43 Estimate) Wat Sol: (User Entered) Wat Sol: (PhysProp DB exp value)

----------------------------- --------- Values used to Generate ECOSAR Profile

-------------------------------------- Log Kow: 1.457 (EPISuite Kowwin v1.68 Estimate)

### Page 43 of 51

Wat Sol: 3556 (mg/L, EPISuite WSKowwin v1.43 Estimate)

------------------------------------------------ Av ailable Measured Data from ECOSAR Training Set

------------------------------------------------

No Data Available

-------------------------------------- ECOSAR v1.1 Class

- specific Estimations

-------------------------------------- Phenols, Poly

- acid

********************************************************************

******* * NOTE: METAL ([Na], [Li] or [K]) HAS BEEN REMOVED FOR PROPER ESTIMATION. * * SALTS SHOULD BE ENTERED IN ECOSAR AS THE NEUTRAL NON

- SALT FORM OF THE * * MOLECULE CORRESPONDI NG TO EITHER THE FREE BASE OR CONJUGATE ACID.

* * SEE HELP MENU FOR MORE INFORMATION

*

********************************************************************

*******

Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

=========================== ================== ======== ======

==========

-- > Acid moeity found: Predicted values multiplied by 10

Phenols, Poly

- acid : Fish 96

- hr LC50 313.933 Phenols, Poly

- acid : Daphnid 48

- hr LC50 3052.885 Phenols, Poly

- acid : Green Algae 96

- hr EC50 88.978 Phenols, Poly

- acid : Fish ChV 187.693 Phenols, Poly

- acid : Daphnid ChV 1180.047 Phenols, Poly

- acid : Green Algae ChV 10.986

=========================== ================== ======== ======

==========

### Page 44 of 51

Neutral Organic SAR : Fish 96

- hr LC50 702.960 (Baseline Toxicity) : Daphnid 48

- hr LC50 379.616 : Green Algae 96

- hr EC50 229.808 : Fish ChV 64.764 : Daphnid ChV 32.197 : Green Algae ChV 53.821

Note: * = asterisk designa tes: Chemical may not be soluble enough
to measure this predicted effect. If the effect level exceeds the water solubility by 10X, typically no effects at saturation (NES) are reported.

------------------------------ Class Speci fic LogKow Cut

- Offs

------------------------------ If the log Kow of the chemical is greater than the endpoint specific
cut

- offs presented below, then no effects at saturation are expected for those endpoints.

Phenols, Poly :

-------------- Maximum LogKo w: 7.0 (Fish LC50) Maximum LogKow: 5.5 (Daphnid LC50) Maximum LogKow: 6.4 (EC50) Maximum LogKow: 8.0 (ChV)

Baseline Toxicity SAR Limitations:

--------------------------------- Maximum LogKow: 5.0 (Fish 96

- hr LC50; Daphnid LC50) Maximum LogKow: 6.4 (Green Algae EC50) Maximum LogKow: 8.0 (ChV)

--------------------------------------------------------------------

----------



### D. 

### EcoSAR estimates Bx

- 180 properties

### Page 45 of 51

ECOSAR Version 1.11 Results Page

SMILES : O=C(c2c(O)cccc2Oc1nc(OC)cc(OC)n1)O User SMILES : C(=O)(c1c(O)cccc1Oc1nc(OC)cc(OC)n1)O{

- }.[Na]{+} CHEM : Bx

- 180 CAS Num: ChemID1: MOL FOR: C13 H12 N2 O6 MOL WT : 292.25 Log Kow: 1.723 (EPISuite Kowwin v1.68 Estimate) Log Kow: (User Entered) Log Kow: (PhysProp DB exp value

- for comparison only) Melt Pt: (User Entered for Wat Sol estimate) Melt Pt: (deg C, PhysProp DB exp value for Wat Sol estimate) Wat Sol: 1750 (mg/L, EPISuite WSKowwin v1.43 Estimate) Wat Sol: (User Entered) Wat Sol: (PhysProp DB exp value)

-------------------------------------- Values used to Generate ECOSAR Profile

-------------------------------------- Log Kow: 1.723 (EPISuite Kowwin v1.68 Estimate) Wat Sol: 1750 (mg/L, EPISuite WSKowwin v 1.43 Estimate)

------------------------------------------------ Available Measured Data from ECOSAR Training Set

------------------------------------------------

No Data Available

-------------------------------------- ECOSAR v1.1 Class

- specific E stimations

-------------------------------------- Phenols

- acid

********************************************************************

******* * NOTE: METAL ([Na], [Li] or [K]) HAS BEEN REMOVED FOR PROPER ESTIMATION. * * SALTS SHOULD BE ENTERED IN ECOSAR A S THE NEUTRAL NON

- SALT FORM OF THE * * MOLECULE CORRESPONDING TO EITHER THE FREE BASE OR CONJUGATE ACID.

* * SEE HELP MENU FOR MORE INFORMATION

*

************************************************************** ******

*******

### Page 46 of 51

Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

=========================== ================== ======== ======

==========

-- > A cid moeity found: Predicted values multiplied by 10

Phenols

- acid : Fish 96

- hr LC50 824.765 Phenols

- acid : Daphnid 48

- hr LC50 219.483 Phenols

- acid : Green Algae 96

- hr EC50 1037.057 Phenols

- acid : Fish ChV 79.946 Phenols

- acid : Daphnid ChV 41.773 Phenols

- acid : Green Algae ChV 488.9 09 Phenols

- acid : Fish (SW) 96

- hr LC50 390.944 Phenols

- acid : Earthworm 14

- day LC50 3524.866 * Phenols

- acid : Lemna gibba 7

- day EC50 631.799

=================== ======== ================== ======== ======

========== Neutral Organic SAR : Fish 96

- hr LC50 425.988 (Baseline Toxicity) : Daphnid 48

- hr LC50 235.764 : Green Algae 96

- hr EC50 157.980 : Fish ChV 40.400 : Daphnid ChV 21.411 : Green Algae ChV 39.079

Note: * = asterisk designates: Chemical may not be soluble enough
to measure this predicted effect. If the effect level exceeds the water solubility by 10X, typically no effects at saturation (NES) are reported.

--- --------------------------- Class Specific LogKow Cut

- Offs

### Page 47 of 51

------------------------------ If the log Kow of the chemical is greater than the endpoint specific
cut

- offs presented below, then no effects at saturation are expected for those endpoints.

Pheno ls:

------- Maximum LogKow: 7.0 (Fish 96

- hr LC50, Daphnid LC50) Maximum LogKow: 6.4 (Earthworm, Lemna) Maximum LogKow: 7.0 (Green Algae EC50 Maximum LogKow: 8.0 (ChV) Maximum LogKow: 5.0 (Fish (SW) 96

- hr LC50, Mysid)

Baseline Toxicity SAR Limitations:

-- ------------------------------- Maximum LogKow: 5.0 (Fish 96

- hr LC50; Daphnid LC50) Maximum LogKow: 6.4 (Green Algae EC50) Maximum LogKow: 8.0 (ChV)

--------------------------------------------------------------------

----------



### E. 

### EcoSAR estimates 2,6

- DBA properties

ECOSAR Version 1.11 Results Page

SMILES : O=C(O)c1c(O)cccc1O CHEM : 26DBA CAS Num: ChemID1: MOL FOR: C7 H6 O4 MOL WT : 154.12 Log Kow: 1.765 (EPISuite Kowwin v1.68 Estimate) Log Kow: (User Entered) Log Kow: 2.20 (PhysProp DB exp value

- for comparison only) Melt Pt: (User Entered for Wat Sol estimate) Melt Pt: 165.00 (deg C, PhysProp DB exp value for Wat Sol est, 165 dec) Wat Sol: 7177 (mg/L, EPISuite WSKowwin v1.43 Estimate) Wat Sol: (User Entered) Wat Sol: 9560 (mg/L, PhysProp DB exp value)

-------------------------------------- Values used to Generate ECOSAR Profile

-------------------------------------- Log Kow: 1.765 (EPISuite Kowwin v1.68 Estimate) Wat Sol: 9560 (mg/L, PhysProp DB exp value)

### Page 48 of 51

------------------------------------------------ Available Measured Data from ECOSAR Training Set

------------------------------------------------

No Data Available

-------------------------------------- ECOSAR v1.1 Class

- specific Estimations

-------------------------------------- Phenols, Poly

- acid

Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

======= ==================== ================== ======== ======

==========

-- > Acid moeity found: Predicted values multiplied by 10

Phenols, Poly

- acid : Fish 96

- hr LC50 114.013 Phenols, Poly

- acid : Daphnid 48

- hr LC50 981.077 Phenols, Poly

- acid : Green Algae 96

- hr EC50 38.787 Phenols, Poly

- acid : Fish ChV 65.046 Phenols, Poly

- acid : Daphnid ChV 372.2 17 Phenols, Poly

- acid : Green Algae ChV 5.012

=========================== ================== ======== ======

========== Neutral Organic SAR : Fish 96

- hr LC50 206.049 (Baseline Toxicity) : Daphnid 48

- hr LC50 114.479 : Green Algae 96

- hr EC50 77.944 : Fish ChV 19.631 : Daphnid ChV 10.509 : Green Algae ChV 19.447

Note: * = asterisk designates: Chemical may not be soluble enough
to measure this predicted effect. If the effect level exceeds the water solubility by 10X, typically no effects at saturation (NES)

### Page 49 of 51 are reported.

------------------------------ Class Specific LogKow Cut

- Offs

------------------------------ If the log Kow of the chemical is greater than the endpoint specif ic
cut

- offs presented below, then no effects at saturation are expected for those endpoints.

Phenols, Poly :

-------------- Maximum LogKow: 7.0 (Fish LC50) Maximum LogKow: 5.5 (Daphnid LC50) Maximum LogKow: 6.4 (EC50) Maximum LogKow: 8.0 (ChV)

Baseline Toxicity SAR Limitations:

--------------------------------- Maximum LogKow: 5.0 (Fish 96

- hr LC50; Daphnid LC50) Maximum LogKow: 6.4 (Green Algae EC50) Maximum LogKow: 8.0 (ChV)

--------------------------------------------------------------------

------ ----



### F. 

### EcoSAR estimates MeBa

ECOSAR Version 1.11 Results Page

SMILES : c1(O)cc(OC)nc(O)n1 CHEM : MeBa CAS Num: ChemID1: MOL FOR: C5 H6 N2 O3 MOL WT : 142.12 Log Kow: 1.016 (EPISuite Kowwin v1.68 Estimate) Log Kow: (User Entered)
L og Kow: (PhysProp DB exp value

- for comparison only) Melt Pt: (User Entered for Wat Sol estimate) Melt Pt: (deg C, PhysProp DB exp value for Wat Sol estimate) Wat Sol: 1.114E+004 (mg/L, EPISuite WSKowwin v1.43 Estimate)
Wa t Sol: (User Entered) Wat Sol: (PhysProp DB exp value)

### Page 50 of 51

-------------------------------------- Values used to Generate ECOSAR Profile

-------------------------------------- Log Kow: 1.016 (EPISuite Kowwin v1.68 Estimate) Wat S ol: 1.114E+004 (mg/L, EPISuite WSKowwin v1.43 Estimate)

------------------------------------------------ Available Measured Data from ECOSAR Training Set

------------------------------------------------

No Data Available

-------------------------- ------------ ECOSAR v1.1 Class

- specific Estimations

-------------------------------------- Phenols, Poly

Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

=========================== ================== ======== ======

========== Phenols, Poly : Fish 96

- hr LC50 29.366 Phenols, Poly : Daphnid 48

- hr LC50 340.294 Phenols, Poly : Green Algae 96

- hr EC50 6.407 Phenols, Poly : Fish ChV 18.777 Phenols, Poly : Daphnid ChV 135.097 Phenols, Poly : Green Algae ChV 0.741

=========================== ================== ======== ======

========== Neutral Organic SAR : Fish 96

- hr LC50 893.984 (Baseline Toxicity) : Daphnid 48

- hr LC50 463.506 : Green Algae 96

- hr EC50 237.106 : Fish ChV 78.500 : Daphnid ChV 35.099 : Green Algae ChV 50.715

Note: * = asterisk designates: Chemical may not be soluble enough
to

### Page 51 of 51 measure this predicted effect. If the effect level exceeds the water solubil ity by 10X, typically no effects at saturation (NES) are reported.

------------------------------ Class Specific LogKow Cut

- Offs

------------------------------ If the log Kow of the chemical is greater than the endpoint specific
cut

- offs
presen ted below, then no effects at saturation are expected for those endpoints.

Phenols, Poly :

-------------- Maximum LogKow: 7.0 (Fish LC50) Maximum LogKow: 5.5 (Daphnid LC50) Maximum LogKow: 6.4 (EC50) Maximum LogKow: 8.0 (ChV)

Baseline Toxicity SAR Limi tations:

--------------------------------- Maximum LogKow: 5.0 (Fish 96

- hr LC50; Daphnid LC50) Maximum LogKow: 6.4 (Green Algae EC50) Maximum LogKow: 8.0 (ChV)

--------------------------------------------------------------------

----------