Test Material: Propyzamide (Pronamide, RH-23315) 

MRID: 48599207 

Title: 

Method Validation Study for the Determination of Residues of 

Propyzamide and its Metabolites in Surface Water, Ground Water and 

Drinking Water by Liquid Chromatography with Tandem Mass 

Spectrometry 

MRID: 48690302 

Title: 

Independent Laboratory Validation of Enforcement Method for the 

Determination of Propyzamide and its RH-24644, RH-24655, RH24580, RH-26059, and UK1 Metabolites in Water (surface water, ground 

water and drinking water) by Liquid Chromatography with Tandem 

Mass Spectrometry 

EPA PC Code: 101701 

OCSPP Guideline: 850.6100 

For CDM Smith 

 

Primary Reviewer: Lynne Binari Signature: 

 Date: 8/11/14 

Secondary Reviewer: Lisa Muto Signature: 

Date: 8/11/14 

 

QC/QA Manager: Joan Gaidos Signature: 

Date: 8/11/14 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 2 of 12 

Analytical method for propyzamide and its transformation products RH-24644, RH-24655, 

RH-24580, RH-26059 and UK1 in water 

Reports: ECM: EPA MRID No.: 48599207. Grotenhuis, A. and Q. Li. 2011. Method 

Validation Study for the Determination of Residues of Propyzamide and its 

Metabolites in Surface Water, Ground Water and Drinking Water by Liquid 

Chromatography with Tandem Mass Spectrometry. Dow AgroSciences 

Study No.: 110587. Report prepared, sponsored, and submitted by Dow 

AgroSciences LLC, Indianapolis, Indiana; 81 pages. Final report issued 

September 6, 2011. 

ILV: EPA MRID No. 48690302. Robaugh, D. 2011. Independent Laboratory 

Validation of Enforcement Method for the Determination of Propyzamide 

and its RH-24644, RH-24655, RH-24580, RH-26059, and UK1 Metabolites 

in Water (surface water, ground water and drinking water) by Liquid 

Chromatography with Tandem Mass Spectrometry. Pyxant Labs Study No.: 

2340. Dow AgroSciences Study No.: 110676. Report prepared by Pyxant 

Labs Inc., Colorado Springs, Colorado, sponsored and submitted by Dow 

AgroSciences LLC, Indianapolis, Indiana; 226 pages. Final report issued 

September 12, 2011. 

Document No.: MRIDs 48599207 & 48690302 

Guideline: 850.6100 

Statements: ECM: The study was conducted in accordance with USEPA FIFRA Good 

Laboratory Practice (GLP) standards, with the exception that GLP 

characterizations of test substances RH-24655 and RH-26059 occurred 

during the course of the study rather than prior to experiment initiation (p. 3 

of MRID 48599207). Signed and dated No Data Confidentiality, GLP, and 

Quality Assurance statements were provided (pp. 2-4). A statement of the 

authenticity of the study report was included with the quality assurance 

statement (p. 4). 

ILV: The study was conducted in accordance with USEPA FIFRA GLP 

standards (p. 3 of MRID 48690302). Signed and dated No Data 

Confidentiality, GLP, and Quality Assurance statements were provided (pp. 

2-4). A statement of the authenticity of the study report was included with 

the quality assurance statement (p. 4). 

Classification: This analytical method is classified as supplemental. For all analytes, 

performance data to validate the method at 10x LOQ were not reported. 

Sufficient results to validate the confirmatory method were not provided. 

Recommended modifications to the ECM were not implemented in an 

amended final ECM report. ILV performance data for 0.05 μg/L (LOQ) 

fortified UK1 (RH-23801) in ground water did not meet OCSPP Guideline 

850.6100 criteria. The waters used in the ILV were not characterized. 

PC Code: 101701 

Reviewer: Karen Milians, Chemist Signature: 

Date: 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 3 of 12 

Executive Summary

This analytical method, Dow AgroSciences Study No. 110587, is designed for the quantitative 

determination of propyzamide and its transformation products RH-24644, RH-24655, RH-24580, 

RH-26059, and UK1 (RH-23801) in water using LC/MS/MS. For all analytes, performance data 

to validate the method at 10x LOQ were not reported. The LOQ is less than the lowest 

toxicological level of concern in water. The ILV validated the quantitation ion method after two 

trials, with the exception of 0.05 μg/L (LOQ) UK1 fortified ground water (RSD 22%). Sufficient 

results to validate the confirmatory method were not provided. Modifications to improve 

precision and repeatability of the method were made to the ECM by the independent laboratory; 

however, the recommended modifications were not implemented in an amended final ECM 

report. The waters used in the ILV were not characterized. 

Table 1. Analytical Method Summary 

Analyte(s) 

by Pesticide

MRID

EPA 

Review Matrix Method Date(dd/mm/yyyy) Registrant Analysis

Limit of 

Quantitation

(LOQ)

Environmental 

Chemistry 

Method

Independent 

Laboratory 

Validation

Propyzamide 

48599207 48690302 Water 06/09/2011 

Dow 

AgroSciences 

LLC 

LC/MS/MS 

0.05 μg/L 

RH-24644 0.05 μg/L 

RH-24655 0.05 μg/L 

RH-24580 0.05 μg/L 

RH-26059 0.05 μg/L 

UK1 

(RH-23801) 0.05 μg/L 

I. Principle of the Method 

Water (4.0 mL) was combined with acetonitrile (1.0 mL), vortexed for a few seconds to mix, 

then centrifuged (2,000 rpm, 10 minutes; p. 20 of MRID 48599207). An aliquot (1.0 mL) was 

transferred to an autosampler vial and analyzed by LC/MS/MS. 

Samples were analyzed for propyzamide (RH-23315) and its products RH-24644, RH-24655, 

RH-24580, RH-26059 and UK1 by HPLC (Zorbax SB-C8, 4.6 mm x 75 mm, 3.5 μm column) 

using a mobile phase of (A) acetonitrile with 0.1% formic acid and (B) water with 0.1% formic 

acid [percent A:B at 0 min. 10:90, 3-5 min. 100:0, 5:15-7 min. 10:90) with MS/MS-ESI 

(electrospray ionization) detection and multiple reaction monitoring (MRM); positive ion mode 

for propyzamide, RH-24644, RH-24655, RH-24580 and UK1, and negative ion mode for RH26059 (pp. 11, 15-18 of MRID 48599207). Injection volume was 30 μL. Analytes were 

identified using two ion transitions; one for quantitation (Q) and one for confirmation (C; p. 21 

of MRID 48599207). Ion transitions monitored were as follows: m/z 256→190 (Q) and m/z

258→192 (C) for propyzamide, m/z 256→173 (Q) and m/z 258→109 (C) for RH-24644, m/z

258→190 (Q) and m/z 258→173 (C) for RH-24655, m/z 274→173 (Q) and m/z 274→109 (C) 

for RH-24580, m/z 288→188 (Q) and m/z 290→190 (C) for RH-26059, and m/z 222→156 (Q) 

and m/z 222→139 (C) for UK1. 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 4 of 12 

In the ILV, the autosampler temperature was reduced from ambient to ca. 15°C due to possible 

stability issues with the analytes (p. 34; Appendix B, p. 218 of MRID 48690302); no other 

modifications were reported. However, the ILV considered, in addition to autosampler 

temperature, the use of syringes and glass pipettes to fortify samples (analytes may adhere to 

plastic) and daily preparation of the mixed calibration standards to be critical method steps (p. 34 

of MRID 48690302). 

The LOQ and LOD for all analytes were the same in the ECM and ILV at 0.05 μg/L (0.05 

ng/mL) and 0.015 μg/L (0.015 ng/mL), respectively (p. 24 of MRID 48599207; p. 33 of MRID 

48690302). 

II. Recovery Findings 

ECM (MRID 48599207): Mean recoveries and relative standard deviations (RSDs) were within 

guideline requirements (mean 70-120%; RSD ≤20%) for analysis of propyzamide, RH-24580, 

RH-24644, RH-24655, RH-26059 and UK1 in ground water, surface water and drinking water at 

fortification levels of 0.05 μg/L (LOQ) and 1.0 μg/L (20x LOQ; Tables 20-22, pp. 48-50). 

Recoveries from samples fortified at 0.015 μg/L (LOD) ranged (matrices combined) from 83-

112% for propyzamide, 87-119% for RH-24580, 74-103% for RH-24644, 90-104% for RH24655, 87-99% for RH-26059, and 87-106% for UK1 (DER Attachment 2). Performance data 

(recovery results) from confirmation ion analyses were not reported. Confirmation ratio 

differences [comparison of the confirmation ratio (confirmation ion peak area/quantitation ion 

peak area) of the recovery sample with the average confirmation ratio of the standards, p. 22] 

were ≤20% for all analytes at both fortification levels in the three water matrices, with the 

exception of one 0.05 μg/L RH-24655 fortified surface water sample (Tables 2-19, pp. 30-47). 

The water matrices were well characterized (p. 19). 

ILV (MRID 48690302): Mean recoveries and relative standard deviations (RSDs) were within 

guideline requirements (mean 70-120%; RSD ≤20%) for analysis of propyzamide, RH-24580, 

RH-24644, RH-24655, RH-26059 and UK1 in ground water, surface water and drinking water at 

fortification levels of 0.05 μg/L (LOQ) and 1.0 μg/L (20x LOQ), with the exception of 0.05 μg/L 

(LOQ) UK1 fortified ground water (RSD 22%; Tables 19-36, pp. 55-72). Performance data 

(recovery results) from confirmation ion analyses were not reported. The water matrices were not 

characterized, but were reported as obtained from Dow AgroSciences (p. 23). The method for 

quantitation ion analyses was validated, with modifications, after two trials, with the exception of 

0.05 μg/L (LOQ) UK1 fortified ground water (pp. 31-33). 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 5 of 12 

Table 2. Initial Validation Method Recoveries for Propyzamide and Its Transformation 

Products in Water

Analyte Fortification 

Level (μg/L)

Number 

of Tests

Recovery 

Range (%) 

Mean 

Recovery (%)

Standard 

Deviation (%) 

Relative Standard 

Deviation (%)

Ground (Well) Water 

Propyzamide 

(RH-23315) 

0.015 (LOD) 2 94, 101 * * * 

0.05 (LOQ) 6 85-116 99 13.3 13.5 

1.0 6 96-115 103 7.5 7.3 

RH-24580 

0.015 (LOD) 2 105, 117 * * * 

0.05 (LOQ) 6 95-111 102 5.4 5.3 

1.0 6 100-108 103 2.8 2.7 

RH-24644 

0.015 (LOD) 2 91, 93 * * * 

0.05 (LOQ) 6 81-115 96 14.5 15.1 

1.0 6 96-120 107 8.4 7.8 

RH-24655 

0.015 (LOD) 2 92, 100 * * * 

0.05 (LOQ) 6 96-105 101 2.9 2.9 

1.0 6 98-104 101 2.1 2.1 

RH-26059 

0.015 (LOD) 2 96, 99 * * * 

0.05 (LOQ) 6 92-96 93 1.6 1.7 

1.0 6 97-99 98 0.8 0.8 

UK1 

(RH-23801) 

0.015 (LOD) 2 99, 103 * * * 

0.05 (LOQ) 6 95-111 100 5.7 5.7 

1.0 6 99-107 103 3.1 3.0 

Surface (Pond) Water 

Propyzamide 

(RH-23315) 

0.015 (LOD) 2 83, 112 * * * 

0.05 (LOQ) 6 92-118 100 9.0 9.0 

1.0 6 98-118 107 7.8 7.3 

RH-24580 

0.015 (LOD) 2 103, 119 * * * 

0.05 (LOQ) 6 84-112 100 9.7 9.7 

1.0 6 97-104 101 2.4 2.4 

RH-24644 

0.015 (LOD) 2 77, 99 * * * 

0.05 (LOQ) 6 91-114 98 8.2 8.4 

1.0 6 101-122 108 8.9 8.2 

RH-24655 

0.015 (LOD) 2 96, 104 * * * 

0.05 (LOQ) 6 88-103 95 5.1 5.3 

1.0 6 95-102 97 2.1 2.2 

RH-26059 

0.015 (LOD) 2 92, 99 * * * 

0.05 (LOQ) 6 91-95 93 1.8 1.9 

1.0 6 93-98 96 1.6 1.7 

UK1 

(RH-23801) 

0.015 (LOD) 2 89, 106 * * * 

0.05 (LOQ) 6 100-104 102 1.3 1.3 

1.0 6 96-101 98 1.7 1.7 

Drinking (Tap) Water 

Propyzamide 

(RH-23315) 

0.015 (LOD) 2 86, 104 * * * 

0.05 (LOQ) 6 87-104 97 7.7 7.9 

1.0 6 95-120 111 10.1 9.1 

RH-24580 0.015 (LOD) 2 87, 93 * * * 

0.05 (LOQ) 6 95-108 102 5.1 5.0 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 6 of 12 

Analyte Fortification 

Level (μg/L)

Number 

of Tests

Recovery 

Range (%) 

Mean 

Recovery (%)

Standard 

Deviation (%) 

Relative Standard 

Deviation (%)

1.0 6 98-108 102 3.7 3.6 

RH-24644 

0.015 (LOD) 2 74, 103 * * * 

0.05 (LOQ) 6 79-100 93 8.8 9.5 

1.0 6 100-120 113 10.2 9.1 

RH-24655 

0.015 (LOD) 2 90, 102 * * * 

0.05 (LOQ) 6 91-104 96 4.6 4.8 

1.0 6 96-106 101 3.7 3.7 

RH-26059 

0.015 (LOD) 2 87, 97 * * * 

0.05 (LOQ) 6 92-97 95 2.1 2.2 

1.0 6 98-102 100 1.5 1.5 

UK1 

(RH-23801) 

0.015 (LOD) 2 87, 92 * * * 

0.05 (LOQ) 6 91-115 104 9.7 9.3 

1.0 6 99-102 100 1.2 1.2 

Data (quantitation ion results) were obtained from Tables 20-22, pp. 48-50 of MRID 48599207 and DER 

Attachment 2 (LOD recovery results). Confirmation ion recovery results were not reported. 

* Not calculated, n = 2. 

Table 3. Independent Validation Method Recoveries for Propyzamide and Its 

Transformation Products in Water1 

Analyte Fortification 

Level (μg/L)

Number 

of Tests

Recovery 

Range (%) 

Mean 

Recovery (%)

Standard 

Deviation (%) 

Relative Standard 

Deviation (%)

Ground Water 

Propyzamide 

(RH-23315) 

0.05 (LOQ) 5 78-117 99 15 16 

1.0 5 73-107 89 12 14 

RH-24580 0.05 (LOQ) 5 91-131 112 15 13 

1.0 5 92-102 97 4.2 4.4 

RH-24644 0.05 (LOQ) 5 89-98 93 3.7 4.0 

1.0 5 96-100 98 1.8 1.8 

RH-24655 0.05 (LOQ) 5 88-112 101 9.0 8.9 

1.0 5 85-105 97 7.5 7.7 

RH-26059 0.05 (LOQ) 5 103-111 107 3.2 3.0 

1.0 5 103-105 104 0.84 0.80 

UK1 

(RH-23801) 

0.05 (LOQ) 5 55-103 85 19 22 

1.0 5 82-107 95 11 12 

Surface Water 

Propyzamide 

(RH-23315) 

0.05 (LOQ) 5 86-98 88 6.5 7.4 

1.0 5 98-108 102 4.8 4.7 

RH-24580 0.05 (LOQ) 5 96-114 107 7.6 7.2 

1.0 5 95-100 98 2.3 2.3 

RH-24644 0.05 (LOQ) 5 99-105 103 2.4 2.3 

1.0 5 90-98 95 3.2 3.4 

RH-24655 0.05 (LOQ) 5 93-101 96 3.3 3.4 

1.0 5 94-103 97 3.5 3.6 

RH-26059 0.05 (LOQ) 5 112-116 114 1.6 1.4 

1.0 5 100-107 104 2.9 2.8 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 7 of 12 

Analyte Fortification 

Level (μg/L)

Number 

of Tests

Recovery 

Range (%) 

Mean 

Recovery (%)

Standard 

Deviation (%) 

Relative Standard 

Deviation (%)

UK1 

(RH-23801) 

0.05 (LOQ) 5 105-124 112 7.4 6.6 

1.0 5 93-105 99 4.2 4.3 

Drinking Water 

Propyzamide 

(RH-23315) 

0.05 (LOQ) 5 80-102 93 9.3 10 

1.0 5 95-103 100 3.3 3.3 

RH-24580 0.05 (LOQ) 5 96-119 110 8.6 7.8 

1.0 5 102-110 107 3.6 3.3 

RH-24644 0.05 (LOQ) 5 93-100 97 2.9 3.0 

1.0 5 91-101 97 4.7 4.8 

RH-24655 0.05 (LOQ) 5 84-107 97 9.0 9.3 

1.0 5 99-104 102 2.0 2.0 

RH-26059 0.05 (LOQ) 5 102-110 105 3.4 3.3 

1.0 5 103-108 105 2.3 2..2 

UK1 

(RH-23801) 

0.05 (LOQ) 5 104-119 108 6.4 5.9 

1.0 5 93-100 96 3.0 3.1 

Data (quantitation ion results) were obtained from Tables 19-36, pp. 55-72 of MRID 48690302. Confirmation ion 

recovery results were not reported. 

1 Water matrices were not characterized; all three control matrices were obtained from Dow AgroSciences (p. 23 of 

MRID 48690302). 

III. Method Characteristics 

In the ECM and ILV, the LOQ and LOD values for propyzamide, RH-24580, RH-24644, RH24655, RH-26059 and UK1 in water were 0.05 μg/L and 0.015 μg/L, respectively (p. 24 of 

MRID 48599207; p. 25 of MRID 48690302). Following the method of Keith, L. H., et al. (see 

section V. References below), the LOD and LOQ for determination of propyzamide and its 

transformation products in water were calculated using the standard deviation from the 0.05 μg/L 

recovery results (p. 24 of MRID 48599207). The LOD was calculated as three times the standard 

deviation (3s), and the LOQ was calculated as ten times the standard deviation (10s) of the 

recovery results (Table 23, p. 51 of MRID 48599207). The calculated values support the LOQ 

and LOD established for the study. 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 8 of 12 

Table 4. Method Characteristics 

Propyzamide RH-24580 RH-24644 RH-24655 RH-26059 UK1 

Limit of Quantitation 

(LOQ) 0.05 μg/L 0.05 μg/L 0.05 μg/L 0.05 μg/L 0.05 μg/L 0.05 μg/L

Limit of Detection 

(LOD) 0.015 μg/L 0.015 μg/L 0.015 μg/L 0.015 μg/L 0.015 μg/L 0.015 μg/L

Linearity (Power fit 

calibration curve r 

and concentration 

range)1

Surface Water 

r = 0.9991 

(0.015-2.0 

μg/L) 

r = 0.9968 

(0.015-2.0 

μg/L) 

r = 0.9978 

(0.015-2.0 

μg/L) 

r = 0.9984 

(0.015-2.0 

μg/L) 

r = 0.9999 

(0.05-2.0 

μg/L) 

r = 0.9927 

(0.015-2.0 

μg/L) 

Ground Water 

r = 0.9963 

(0.015-2.0 

μg/L) 

r = 0.9936 

(0.015-2.0 

μg/L) 

r = 0.9986 

(0.015-2.0 

μg/L) 

r = 0.9955 

(0.015-2.0 

μg/L) 

r = 0.9999 

(0.015-2.0 

μg/L) 

r = 0.9995 

(0.015-2.0 

μg/L) 

Drinking Water 

r = 0.9999 

(0.05-2.0 

μg/L) 

r = 0.9937 

(0.015-2.0 

μg/L) 

r = 0.9996 

(0.015-2.0 

μg/L) 

r = 0.9974 

(0.015-2.0 

μg/L) 

r = 0.9999 

(0.015-2.0 

μg/L) 

r = 0.9982 

(0.015-2.0 

μg/L) 

Repeatable 

Yes Yes Yes Yes Yes 

Yes, except at 

LOQ in 

ground water 

(RSD 22%) 

Reproducible 

Yes Yes Yes Yes Yes 

Yes, except in 

ground water 

(RSD 22%) 

Specific Could not be determined; recovery results from confirmation ion analyses were not provided. 

Data were obtained from p. 25; Figures 1-18, pp. 91-108 of MRID 48690302. 

1 Reviewer-calculated r2

 values for the provided ILV calibration curves (matrices combined) were 0.9926-0.999 for 

propyzamide, 0.9873-0.9936 for RH-24580, 0.9955-0.9991 for RH-24644, 0.9909-0.9968 for RH-24655, 0.9998 

(n = 3) for RH-26059, and 0.9855-0.9989 for UK1 (DER Attachment 2). The study authors reported calculated r2

values (quantitation ion analyses) of 0.9991, 0.9985, 0.9998, 0.9997, 0.9998, 0.9998 for propyzamide, RH-24644, 

RH-24655, RH-24580, RH-26059 and UK1, respectively (p. 23 of MRID 48599207). Reported ECM r2

 values 

could not be verified by reviewer because complete calibration data were not reported (Figures 7-13, pp. 58-62 of 

MRID 48599207). 

IV. Method Deficiencies and Reviewer’s Comments 

1. For both the ECM and ILV, performance data at 10x LOQ were not reported to validate 

the method. A validation sample set should consist of, at a minimum, a reagent blank, 

two unspiked matrix control samples, five matrix control samples spike at the LOQ, and 

five matrix control samples spiked at 10x LOQ for each analyte and matrix. 

2. For the ILV, the following fortifications using the quantitation ion method did not meet 

OCSPP Guideline 850.6100 criteria for precision and accuracy (mean recoveries for 

replicates at each spiking level between 70% and 120% and relative standard deviations 

(RSD) ≤20%): 0.05 μg/L (LOQ) fortified UK1 in ground water (RSD 22%; Table 30, p. 

66 of MRID 48690302). 

3. For both the ECM and ILV, insufficient performance data (no recovery results) were 

provided to validate the confirmation ion analyses. For the ECM, only confirmation ratio 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 9 of 12 

difference results, i.e. comparison of the confirmation ratio (confirmation ion peak 

area/quantitation ion peak area) of the recovery sample with the average confirmation 

ratio of the calibration standards, were reported (pp. 21-22; Tables 2-19, pp. 30-47 of 

MRID 48599207). Chromatograms from confirmation ion analyses of 0.05 μg/L (LOQ) 

and 1.0 μg/L (20x LOQ) fortified samples were provided; however, the respective 

calibration curves to allow calculation of recoveries were not provided (Figures 15-16, 

pp. 65-68; Figures 18-19, pp. 71-74; Figures 21-22, pp. 77-80 of MRID 48599207). 

For the ILV, no results (recovery results or chromatograms) from confirmation ion 

analyses were reported. 

4. The ILV implemented several modifications to the ECM to improve precision and 

repeatability of the method (p. 34; Appendix B, pp. 217-218 of MRID 48690302). The 

autosampler temperature was reduced from ambient to ca. 15°C due to possible stability 

issues with the analytes. Additionally, the ILV considered the use of syringes and glass 

pipettes to fortify samples (analytes may adhere to plastic) and daily preparation of the 

mixed calibration standards to be critical method steps. The modifications recommended 

by the independent laboratory were not implemented in an amended final ECM report. 

5. The waters used in the ILV were not characterized. The water matrices were reported as 

obtained from Dow AgroSciences (p. 23 of MRID 48690302). 

6. In the ECM, the stability of the analytes prepared in acetonitrile and stored at -20°C was 

investigated (pp. 24-25 of MRID 48599207). Quantitation ion analyses found 

propyzamide (RH-23315, 96.4% recovered) and RH-24580 (100.2%) were stable up to 

57 days, RH-24644 (96.2%), RH-24655 (96.4%) and RH-26059 (110.2%) up to 56 days 

and UK1 (97.8%) up to 41 days. Recoveries from confirmation ion analyses were also 

determined, but not reported. 

In addition, stability of the sample extracts was investigated (p. 25 of MRID 48599207). 

Following initial analyses, the samples were stored at 4°C for 3-6 days, then re-analyzed. 

Quantitation ion analyses determined mean recoveries for all analytes ranged from 91-

107% with RSDs of 1.3-7.0%. Propyzamide (RH-23315), RH-24644, RH-24655, RH24580 and UK1 were found to be stable up to 3 days and RH-26059 up to 6 days after 

sample preparation. 

7. It was reported for the ILV that one batch of thirty-eight samples required approximately 

four person hours over one calendar day to complete the preparation (p. 34 of MRID 

48690302). Instrument analysis of water samples was three hours. Data manipulation 

required an additional one to two person hours. Initial solution preparation required 

approximately three person hours. 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 10 of 12 

V. References 

Keith, L. H.; Crummett, W.; Deegan, J., Jr.; Libby, R. A.; Taylor, J. K.; Wentler, G. Anal. Chem.

1983, 55, 2210-2218 (p. 27 of MRID 48599207). 

U.S. Environmental Protection Agency. 2012. Ecological Effects Test Guidelines, OCSPP 

850.6100, Environmental Chemistry Methods and Associated Independent Laboratory 

Validation. Office of Chemical Safety and Pollution Prevention, Washington, DC. EPA 

712-C-001. 

40 CFR Part 136. Appendix B. Definition and Procedure for the Determination of the Method 

Detection Limit-Revision 1.11, pp. 317-319. 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 11 of 12 

Attachment 1: Chemical Names and Structures 

Propyzamide (Kerb, pronamide, RH-23315, RH-3315, RH-315) 

 

IUPAC Name: 3,5-Dichloro-N-(1,1-dimethylprop-2-ynyl)benzamide 

CAS Name: 3,5-Dichloro-N-(1,1-dimethyl-2-propynyl)-benzamide 

CAS Number: 23950-58-5 

SMILES String: CC(C)(NC(=O)c1cc(Cl)cc(Cl)c1)C#C (EPI Suite, v3.12 SMILES).

RH-24644 (RH-4644) 

IUPAC Name: 3,5-Dichloro-N-(1,1,2-trimethylprop-2-enyl)benzamide 

CAS Name: 2-(3,5-Dichlorophenyl)-4,4-dimethyl-5-methylene oxozoline 

CAS Number: 29918-40-9 

SMILES String: Not reported. 

RH-24655

IUPAC Name: 3,5-Dichloro-N-(1,1-dimethylpropenyl)benzamide 

CAS Name: Not reported. 

CAS Number: Not reported. 

SMILES String: Not reported. 
Propyzamide (PC 101701) MRIDs 48599207 (ECM)/ 48690302 (ILV) 

Page 12 of 12 

RH-24580 (RH-4580) 

IUPAC Name: 3,5-Dichloro-N-(1,1-dimethyl-2-oxopropyl)benzamide 

CAS Name: N-(1,1-Dimethyl acetonyl)-3,5-dichloro-benzamide 

CAS Number: 29918-41-0 

SMILES String: Not reported. 

 

RH-26059

IUPAC Name: 3-[(3,5-Dichlorobenzoyl)amino]-3-methylbutanoic acid 

CAS Name: Not reported. 

CAS Number: Not reported. 

SMILES String: Not reported. 

RH-23801 (UK1, 3-chloro kerb) 

IUPAC Name: 3-Chloro-N-(1,1-dimethylpropynyl)benzamide 

CAS Name: Not reported. 

CAS Number: Not reported. 

SMILES String: Not reported.