## 1 OFFICE OF

PREVENTION, PESTICIDES AND

TOXIC SUBSTANCES 

### UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON D.C., 20460



## PC Code: 129112

Chemical: Trifloxystrobin

DP Barcode : D378918

December 17, 2010

MEMORANDUM

SUBJECT: Ecological risk assessment for the new u se of Trifloxystrobin [ Trifloxystrobin

Flowable Fungicide, EPA Reg. No. 264

- 989] on alfalfa seed.

TO: Tony Kish, RM 22

Janet Whitehurst, Risk Review Manager

Fungicide Branch/ Registration Division (7505P)

FROM: Pamela Hurley, Ph. D., Senior Toxicologist

Tiffany Mason, Environmental Engineer

Melissa Panger, Ph.D., Senior Biologist

James Hetrick, Ph.D., Senior Science Advisor

Dana Spatz, Branch Chief

Environmental Risk B ranch 3

Environmental Fate and Effects Division (7507P)

This memorandum summarizes the ecological risks associated with the proposed new use and

increased rates of the fungicide trifloxystrobin (PC code: 129112) [ Trif loxystrobin Flowable

Fungicide, 22% a.i. (EPA Reg. No. 264

- 989)] for alfalfa. The proposed method of applicat ion for

this new product is standard slurry or mist type seed treatment.

Risk Summary

The proposed new application rate for alfalfa is 0.96 fl oz per 100 lbs of seed (0.0047 lb a.i./acre)

and one application per season.

EFED’s assessment of the new trifloxystrobin use rates indicate that there are no LOC

exceedances for either freshwater/marine/estuarine aquatic organisms or terrestrial organisms on

an acute and chronic basis

. For terrestrial plants, the EC 25 

## is > 0.25 lb a.i./A. Using that value,

the LOC for non

- listed terrestrial plants would not be exceeded; however, neither a NOAEC nor

## 2 an EC 05 

## has been established for terrestrial plants; therefore, risk to endangered terrestrial plant

species cannot be precluded.

Most required studies for trifloxystrobin have been submitted and found to be acceptable.

However, an acceptable anaerobic aquatic metabolism study has not been submitted; therefore,

there is uncertainty asso ciated with the persistence and dissipation of trifloxystrobin in anaerobic

aquatic environments. This uncertainty is greater for low pH aquatic environments because

hydrolysis is slower in acidic waters.

For risk to estuarine/marine invertebrates (mysid ) following chronic exposure, t he mysid shrimp

NOAEC of 0.96 ppb was estimated using an acute to chronic ratio derived for daphnids. If the

acute to chronic ratio is not conserved across species, then risk could be over

- or under

e stimated. Also, trifloxys trobin is expected to degrade relatively rapidly in most environments to

the major degradate CGA

- 321113, which is extremely persistent. Therefore, chronic exposure is

more likely to occur to the degradate CGA

- 321113 than to trifloxystrobin. The only chroni c

study on the degradate that has been submitted is a reproduction study in daphnids , thus leaving

an uncertainty in the chronic risk conclusions to other organisms. Nonetheless, trifloxystrobin

has been shown to be more than 3 orders of magnitude more tox ic than the degradate to aquatic

organisms on an acute basis (see Appendix 1) . A similar magnitude difference in toxicity was

also observed in a daphnid reproduction study. Therefore, risk quotients based on chronic

toxicity values from trifloxystrobin stu dies are expected to be protective of organisms exposed to

the major degradate.

Previous Risk Conclusions

Trifloxystrobin has been previously evaluated by EFED for use on numerous commodities

including pome fruits, grapes, cucurbits, peanuts

, and turf (N ew Chemical Review, D244219,

D245281, D254206, D254285, D254286); almonds, fruiting vegetables, hops, potatoes, sugar

beets, and wheat (D254215, D254220, D254222); root vegetable subgroup 1B (except beet and

radish) and leaf petiole subgroup 4B (D286427); barley

, oats, sweet corn, and soybeans

(D315580, D314187, D318621, D318623 , D363130 ) ; grass grown for seed (D332979,

D332982) ; asparagus, tropical crops, radish, root vegetables , and strawberries (D331915,

D339548, D348876). Detailed summaries of these as sessments can be found elsewhere

(D309489, D331915).

Based on previous reviews, EFED concluded that some uses are expected to present risk on an

acute basis to freshwater and/or estuarine/marine aquatic organisms (fish and invertebrates);

including Feder ally

- listed endangered and threatened species. Those uses include turf, cucurbits,

grasses grown for seed, tropical crops, root vegetables, leaf petiole , almonds, fruiting vegetables,

hops, and potatoes. Ecological risks are not expected for any of the ot her registered uses based

on previous assessments.

## 3 Analysis

Trifloxystrobin is a broad spectrum foliar fungicide that is currently used to control certain

diseases in crops including, barley, corn, oats, peanuts, pecans, rice, soybeans, wheat and others

. It also provides both curative and protective properties against a variety of pathogens in a variety

of crops. Trifloxystrobin is classified as an oximinoacetate fungicide in the strobilurin (sterol

inhibitors) class. It belongs to the Qol (Group 11) ta rget site of action group and acts by

interfering with respiration in plant pathogenic fungi and is a potent inhibitor of spore

germination and mycelial growth. It exhibits no known cross

- resistance to other chemical classes

including anilinopyrimidines (A P). Other products containing the active ingredient

trifloxystrobin are Flint ® 

## (EPA Reg. No 264

- 777), S tratego (EPA Reg. No 264

- 779), and

DistinguishTM (EPA Reg. No. 264

- 1027) fungicides.

The proposed label for trifloxystrobin does not give the application rate for the n ew use on

alfalfa in lbsa.i./A, but in floz of product per 100 lbs of seed. Therefore, this had to be converted

to lba.i./A. Likewise, the number of applications is not specified by the label, but one

application per season was chosen based on crop profiles for alfalfa. The lbs of seeds applied per

acre and the incorporation depth werebased upon information found in the literature.

( http://www.uaex.edu/O ther_Areas/publications/pdf/FSA

- 2002.pdf 

## ).

Table 1. Crop, application rate, maximum seasonal rate, and reapplication interval for proposed new use

of trifloxystrobin.

Crop Application Rate Total (season or crop) Reapplication Interval

lba.i./acre No. Applications (days)

Alfalfa 0.0047 1 NA

1 : The label gives the maximum application rate in oz of product/100 lbs seed. This had to be converted to lba.i./A using the following formulas:

) . . (

4

. 453 1 ) ( 6

. 29 .)

. (% ) ( Pr 3

A i a lbs robin trifloxyst

g lb

oz cm density i a A Floz oduct **≅ ×**  **× ×**  **×**



## The application rate, applications per season, and reapplication intervals are used to estimate

exposure concentrations (EECs) which are then compared to the toxicity data (Appendi ces 2 and

4 , respectively).

The EECs are represent ed by the residues of the fungicide trifloxystrobin

. Although i ts major

degradation product (CGA

- 321113) comprises >50% of applied trifloxystrobin in some

dissipation studies, the available data suggest that CGA

- 321113 is considerably less toxic to

aquatic organisms on an acute a nd chronic basis than trifloxystrobin (see Appendix 1).

Therefore, it is

' 

## anticipated that risk quotients based on chronic studies using trifloxystrobin

would be protective of aquatic organisms exposed to CGA

- 321113 on a chronic basis. The

available data do not allow for a comparison of the relative toxicities of trifloxystrobin and the

major degradate in terrestrial organisms. Therefore, based on available data, we assume that the

degradate CGA

- 321113 is less toxic to non

- target organisms than the parent , and the risk

assessment will be based on exposure to the parent compound alone.

## 4 Aquatic Exposures

The EECs in surface water concentrations were estimated with the Tier II PRZM (Version

3.12.2) and EXAMS ( vers ion 2.98.04 .06) models linked by the program PE5 ( V ers ion 01

compiled 07/23/07 ). PRZM simulates pesticide fate and transport as a result of leaching, direct

spray drift, runoff and erosion from an agricultural field

. The EXAMS model simulates pesticide

loading via runoff, erosion, and spray drift int o a “standard” farm pond. Detailed description,

documentation, and direct links for running these models can be found in:

http://www.epa.gov/oppefed1/models/water/index.htm

.

The standard farm pond scenario is used to estimate EECs for ecological exposure. The pond

scenario, represents a 10

- ha corn (all cropped) field that is adjacent to a one hectare by two meter

deep farm pond, which has neither hydraulic inlets nor outlets ( i.e., pesticide cannot leave by

outflow). Weather and agricultural practices are simulated for 30 y ears so that the 10

- year

exceede nce probability (1

- in

- 10 year) at the site can be estimated. The simulation was generated

using 30 years of meteorological da ta, encompassing the years from 1961 to 1990.Guidance for

using the standard farm pond is located at: http://www.epa.gov/pesticides/trac/science/.

The surface water trifloxystrobin EECs wer e estimated for theseed application , using the

maximum application rates and assuming an annual application for thirty years

. Since alfalfa is

a perennial crop that is reseeded approximately every 3

- 5 years , the generated EECs should be

conservative.

T he maximum peak, 21

- day, 60

- day means for the seed treatment application method is

summarized in Table 1. It should be noted that while the Texas Alfalfa scenario had the highest

peak concentration (0.044 μg/L), the highest 21

- day (0.007 μg/L) and 60

- day ( 0.003 μg/L)

means occurred with the North Carolina Alfalfa scenario.

The chemical properties for trifloxystrobin and input values used for PRZM/EXAMS are

summarized in Appendix 3 and the PRZM/EXAMS output file is in Appendix 5

.

## 5 Table 2. PRZM/EXAMS estimated exposure concentrations (EECs) for the proposed new use.

Crop or

Crop

Group Maximum

Application

Rate Total

(season

or crop) Minimum

Application

Interval Scenario

(State crop)

Scenario 1 st

Application

date

(mn

- day) Application

Method EECs

lba.i./acre lba.i./acre (days) Peak 21 day 60 day



### Alfalfa 0.0047 1 NA Caalfalfa 16

- Jan

Ground

(seed

treatment) 0.0102 0.0014 0.0005

ILalfalfaNMC 1

- Jun 0.0108 0.0018 0.0006

Mnalfalfa 1

- Jun 0.0024 0.0006 0.0002

Ncalfalfa 1

- Apr 0.0229 0.0070 0.0025

Paalfalfa 16

- Apr 0.0117 0.0039 0.0014

Txalfalfa 1

- Sep 0.0440 0.0069 0.0024

*Values in bold are the most conservative values.



## Terrestrial Exposures

Terrestrial wildlife exposure estimates are typically estimated for birds and mammals following

the methods of Hoerger and Kenaga (1972) as modified by Fletcher et al. (1994). The conceptual

framework for this method of estimating terrestrial exposure assu mes that organisms are exposed

to a single pesticide residue via dietary routes in a specific exposure scenario. For seed treatment

applications of trifloxystrobin, terrestrial exposure estimates rely on estimation of a dose (mg a.i.

bw

- 1 

## day

- 1 

## ) and availa ble pesticide (mg a.i. ft

- 2 

## ).

Table 3 summarizes the terrestrial EECs for the proposed new use. An application rate of 0.96

fluid ounces of product per 100 pounds of seed was considered in TREX (v1.4.1); 1 application

with a maximum seeding rate of 25 p ounds/acre.

The EECs on seed were converted to an oral dose and compared with the oral toxicity data. The

risk assessment for trifloxystrobin uses upper bound ( i.e ., 90 th 

## percentile) predicted residues as

the measure of exposure. The predicted upper boun d residues that may be expected to occur

immediately following application to alfalfa seed are presented in Table 3.

Table 3. Dose

- based Seed Residue Estimates for Proposed Alfalfa Use Rates

Crop Maximum

Application Rate

lbsa.i./A Maximum Seed

Application Rate

mg a.i./kg seed Avian Nagy

Dose

mg a.i./kg

bw/day Mammalian

Nagy Dose

mg a.i./kg

bw/day Available a.i. mg

a.i. ft

- 2

Alfalfa <0.01 187.28 47.40 39.68 0.05



## TerrPlant (Version 1.1.2) is used to calculate EECs for non

- target plant species inhabiting dry

and semi

- aquatic areas. Parameter values for application rate, drift assumption and incorporation

depth are based upon the use and related application method. For seed treatment, assumption of

drift alone is not used. A runoff value of 0.01 is utilized based on trifloxystrobin’s solubility,

which is classified by TerrPlant as <10 mg/L. These EECs are listed in Table 4.

## 6 Table 4. EECs for Trifloxy strobin. Un its in lbs/A.

Description EEC

Runoff to dry areas 0.000047

Runoff to semi

- aquatic areas 0.00047

Total for dry areas 0.000094

Total for semi

- aquatic areas 0.000517

Effects Data

A tabular summary of the ecotoxicity data for trifloxystrobin is provided in Appendix 1.

Trifloxystrobin is very highly toxic to aquatic organisms on an acute basis. Fish LC 50 

## values

range from 14 to 78 ppb, and invertebrate EC 50 

## values range from 8.6 to 29 ppb. Trifloxystrobin

has been shown to affect survival in fi sh chronic studies at 7.7 ppb and survival and reproduction

in invertebrate reproduction studies at 5.9 ppb. No effects were observed in fish and

invertebrates at 4.3 ppb and 2.8 ppb, respectively.

Trifloxystrobin is highly toxic to aquatic plants base d on the green algae EC 50 

## . The green algae

and duckweed EC 50 

## values were 37 ppb and >1930 ppb, respectively, and the NOAECs were 10

ppb and 410 ppb, respectively.

Trifloxystrobin is practically non

- toxic to birds (LD 50 

## >2000 mg/kg

- bw) and mammals

(LD 50 

## > 5000 mg/kg

- bw) on an acute oral basis. No mortality or signs of toxicity occurred in

these acute studies. Exposure to trifloxystrobin did not induce reproductive effects at the

concentrations evaluated in submitted studies in birds (NOAEC = 320

- 500 ppm ) or mammals

(NOAEC = 1500 ppm).

Trifloxystrobin did not cause >25% inhibition in tier I terrestrial plant toxicity studies at 0.249

lbsa.i./Acre, which is above the maximum proposed application rate being assessed in this

evaluation (0.0047 lbsa.i./Acr e). Therefore, Tier II studies are not required. However, because

inhibition was observed, and the data from a Tier I study are not sufficient to allow for

calculation of a slope, an EC 05 

## , or a NOAEC, risk to endangered plant species cannot be assessed

a nd, therefore, cannot be precluded.

## 7 Risk Estimation

Risk characterization integrates EECs and toxicity estimates to determine whether the proposed

uses pose risk to non

- target species at levels of concern (LOC) to the Agency. In a deterministic

approach, a single point estimate of toxicity is divided by an exposure estimate to calculate a risk

quotient (RQ). The RQ is then compared to Agency LOCs, which serve as criteria for

categorizing potential risk to non

- target organisms (See Appendix 4)

.

None of the acute or chronic RQs calculated for fish (freshwater and estuarine/marine) or aquatic

invertebrates (freshwater and estuarine/marine) or RQs for aquatic plants (vascular and non

vascular) exceed 0.01 for any of the scenarios modeled (see Appendices 2 and 6). Therefore,

none of the aquatic RQs exceeded any of the Agency’s LOCs for aquatic organisms.

Acute terrestrial animal RQs were not calculated for the proposed new uses of trifloxystrobin

since the available acute oral and dietary did not determine definitive acute toxicity thresholds

( i.e., bird LD 50 

## > 2000 mg/kg; bird LC 50 

## > 5050 ppm; mammalian LD 50 

## > 5000 mg/kg). There

were no treatment

- related mortalities in any of the avian or mammalian acute toxicity studies. If

acute RQs were calculated using these data, all avian and mammalian acu te RQs would be l ess

than LOCs. Additionally, the chronic RQs for birds and mammals are 0.59 and 0.12,

respectively (see Appendix 6), which are both below the Agency’s chronic risk LOC for these

taxa.

The acute contact toxicity value for honeybees is >200 ug/bee , which i s practically non

- toxic.

Risk estimations for terrestrial invertebrates is not routinely conducted. Based on the acute

contact toxicity study to honeybees, risk to terrestrial invertebrates is presumed to be negligible.

Using Terrplant and the endpoint of >0.25 lb/A for terrestrial plants, the LOC for non

- listed

terrestrial plants would not be exceeded (See Appendix 6); however, neither a NOAEC nor an

EC 05 

## has been established for terrestrial plants; therefore, risk to endangered terrestrial plant

specie s cannot be precluded.

Risk Description

Non

- target Aquatic Animals and Plants

The acute and chronic LOCs are not exceeded for either freshwater fish or invertebrates

( Appendix 6 ). Minimal risk is presumed for non

- endangered and endangered freshwater fish and

invertebrates from the parent material. Based on the toxicity of CGA

- 321113 (EC 50 

## > 95,000 ppb,

NOAEC = 3,200 ppb for the most sensitive organism, Daphnia magna ), minimal risk to either

freshwater fish or invertebrates is also expected from the primary degradate (MRIDs 44527503

and 44869701 ) . No additional degradate testing is required at this time.

LOCs were not exceeded for acute or chronic endangered species for estuarine/marine

invertebrates or fish for the proposed alfalfa use. Likewise, no chronic LOCs were exceeded.

T he mysid shrimp NOAEC of 0.96 ppb was estimated using an acute to chronic ratio derived

## 8 using acute and chronic studies in daphnids. If the acute to chronic ratio is not conserved across

species, then risk could be over

- or u nder

- estimated.

Aquatic Plants

The LOC is not exceeded for either vascular or non

- vascular aquatic plants (see Appendix 6)

. Minimal risk is presumed for non

- endangered and endangered aquatic plants from the parent

material. Based on the toxicity of CGA

- 321113 (EC 50 

## = 77.1 ppm), minimal risk to aquatic

plants also is expected from the primary degradate (MRID 44527504 ) . No additional degradate

testing is required at this time.

T rifloxystrobin is expected to degrade relatively rapidly in most environments to the major

degradate CGA

- 321113, which is extremely persistent ; therefore, chronic exposure is more

likely to occur with the degradate CGA

- 321113 than with trifloxystrobin. However, the

degradate has been shown to be considerably less toxic than triflox ystrobin in aquatic plants,

fish, and invertebrates on an acute basis, and in aquatic invertebrates on a chronic basis.

Therefore, exposure to CGA

- 321113 is not expected to pose either acute or chronic risk to

aquatic organisms at the Agency’s LOCs.

Non

- target Terrestrial Animals and Plants

As stated previously, a cute terrestrial animal RQs were not calculated for the proposed new uses

of trifloxystrobin since the available acute oral and dietary did not determine definitive acute

toxicity thresholds . When compared to the estimated terrestrial EECs, the acute toxicity

endpoints were all greater than 1/10 of the EECs; therefore, there is minimal concern for listed

birds, reptiles, terrestrial

- phase amphibians or mammals on an acute exposure basis. Si milarly,

following chronic exposure, the LOCs for both birds and mammals were below the LOC.

Therefore, there is minimal concern for listed birds, reptiles, terrestrial

- phase amphibians and

mammals following chronic exposure to trifloxistrobin with the cu rrently proposed seed use.

Again, as stated previously, risk to terrestrial invertebrates is presumed to be negligible.

Risk to non

- listed terrestrial plants is not expected for this use; however, due to the fact that

neither a NOAEC nor an EC 05 

## has be en established for terrestrial plants

, risk to listed terrestrial

plant species cannot be precluded. In addition, EFED notes that another strobilurin fungicide has

been reported to be phytotoxic to fruit and fruit trees at low levels. Therefore, there is a concern

that trifloxystrobin may also be phytotoxic to fruit trees. EFED's current plant toxicity database

does not provide information that would allow an assessment of such damage. The registrant

would need to submit available studies or information on potential phytotoxicity to fruit trees or

other agronomic crops to allow for an analysis of such risks. Finally, there are a number of

incident reports indicating damage to terrestrial plants following application of a trifloxistrobin

formulation (see below and Appendix 7).

## 9 Incident Reports

Sixteen incident reports are available in the Ecological Incident Information System (EIIS, v.

2.1), ranging from the year 2000 to 2004. All are incidents with terrestrial plants. One was

classified as unlikely, 3 were classified as possible and the rest were classified as probable. Six

were with registered uses and the remainder were undetermined. Two reports were with wheat

and grape, 10 with ornamentals and the last two were with a tree farm and with bell pe ppers.

The majority of the incidents were in Florida. The remaining reports were in California, Virginia

and North Dakota. See Appendix 7. In the Aggregate Incident Report, there are 4 minor

incidents with terrestrial plants, ranging from 2006

- 2008. N o incidents were reported in the

AIMS database (American Bird Conservancy).

## 10 A PPENDIX 1.

Ecotoxicity Profile of Trifloxystrobin

Appendix 1. Table 1. Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk

Assessment Comments MRID

Terrestrial Toxicity

Rat acute oral LD 50 

### >5000 mg/kg

- bw Acceptable study. 44496622

Rat 2

- generation

reproduction NOAEL 1500 mg/kg

feed Acceptable study. No treatment

- related

effects were observed at any concentration

tested (up to 1500 ppm feed). 44496710

Bird acute oral LD 50 

### (Mallard Duck, Bobwhite

Quail) LD 50 

### : >2000

- >2250

mg/kg

- bw Acceptable studies. No mortality or

treatment

- related toxic effects occurred in

either species. 44496606

44496608

Bird dietary LC 50 

### (Mallard

duck and Bobwhite quail) >5050 mg/kg

- feed Acceptable study. No mortality or

treatment

- related toxic effects occurred in

either species. 44496607

44496605

Reproductive Toxicity

NOAEL (Bobwhite) 320 mg/kg

- feed Acceptable study. No treatment

- related

effects were observed at any concentration

tested (up to 320 ppm feed). 44496609

Reproductive Toxicity

NOAEL (Mallard duck) 500 mg/kg

- feed Acceptable study. No treatment

- related

effects were observed at any concentration

tested (up to 500 ppm feed). 44496610

Honey Bee Contact LD 50 

### >200 ug/bee Acceptable study. No mortality was

observed. 44496726

Terrestrial Plants (Tier I) None used. EC 25 

### is

greater than the highest

concentration tested

(0.25 lbsa.i./Acre) Supplemental Study. The study is

acceptable; however, it does not allow for

an assessment of risk to endangered species

because a 13% effect was observed in

cabbage. The data do not a llow for

derivation of a NOAEC or an EC 05



### . 44496723



## Aquatic Toxicity

Rainbow Trout LC 50 

### Degradate CGA

- 321113

Bluegill Sunfish LC 50 

### 14 ppb

>106,000 ppb

54 ppb Acceptable study

Supplemental study (insufficient number

of test organisms)

Acceptable study 44502803

44527502

44502802

Daphnia magna EC 50 

### 25 ppb Acceptable studies 44496611

## 11 Appendix 1. Table 1. Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk

Assessment Comments MRID

Degradate CGA

- 321113 >95,000 ppb Acceptable studies 44527503

Sheepshead minnow LC 50 

### 78 ppb Acceptable study 44496612

Oyster shell deposition

EC 50 

### 29 ppb Acceptable study 44496613

Mysid shrimp LC 50 

### 8.6 ppb Acceptable study 44496614

Mysid shrimp NOAEC 0.96 ppb No study submitted. The estuarine/marine

chronic NOAEC was estimated using acute

to chronic ratios (ACRs) derived from

daphnid acute (EC50=25 ppb) and chronic

(NOAEC = 2.8 ppb) toxicity values. The

ACR was 8.9. Therefore, the estimated

NOAEC in mysid shrimp would be 8.6/8.9

= 0.96 ppb. Not

applicable

Rainbow Trout Early Life

Stage NOAEC: 4.3 ppb Acceptable study. The LOAEC was 7.7

ppb based on reduced survival. 44502804

Daphnia magna life cycle

Degradate CGA

- 321113 NOAEC: 2.8 ppb

NOAEC: 3,200 ppb Acceptable study. The LOAEC was 5.9

ppb based on reduced survival and

reproduction.

Supplemental study. Water parameter

measurements and growth endpoints of

weight and length were not measured 44527503

44869701

Green algae

Degradate CGA

- 321113 EC 50 

### : 37 ppb

EC 50 

### : 78,800 ppb Acceptable study. The NOAEC was 10

ppb.

Supplemental study. Test duration was

only 72 hours; a definitive EC50 was not

determined 44496724

44527504

Duckweed ( Lemnagibba ) EC 50 

### : >1930 ppb Acceptable study. The NOAEC was 410

ppb. 44496725

## 12 A PPENDIX2

.

Toxicological Endpoints and Summary of Risks

Appendix 2 . Table 1. Summary of Risks Identified in this Assessment from the Proposed Trifloxystrobin Uses

Ecosystem at

Risk Taxonomic group

(surrogate

species) Assessment

Endpoints a 

### Toxicity Value Used to

Evaluate Assessment

Endpoints b 

### Conclusions Regarding

Potential for the Assessment

Endpoints to be Affected by the

Proposed New Trifloxystrobin

Uses

Terrestrial

ecosystems:

Animals . Treated

and adjacent fields

Plants Birds and Reptiles

. Run

- off or

drift onto an

adjacent field and

runoff onto a low

lying semi

- aquatic

or wetland area (Mallard duck

Bobwhite quail) Survival,

reproduction,

and

growth Survival

LD50: >2000

- >2250 mg/kg

- bw :

Dietary LC50: >5050 ppm (both

species)

Growth and Reproduction

NOAEC: 320 ppm to 500 ppm : Risk is below the Agency’s LOCs

for the assessed endpoints.

Mammals

(Laboratory Rat) Survival

LD50: >5000 mg/kg

- bw :

Growth and Reproduction Risk is below the Agency’s LOCs

for the assessed endpoints.

:

NOAEC: 1500 ppm

Terrestrial plants c 

### Survival and

growth Growth and survival

EC 25 

### : >0.25 lbsa.i./Acre : Because neither a NOAEC nor an

EC 05 

### has been established, risk to

endangered plant species cannot

be precluded.

Insects Survival (honey

bees) Survival

LD 50 

### : >200 ug/bee : Risk to pollinators appears to be

lower than levels that are of

concern to the Agency.

Aquatic

ecosystems: Tier

II environment is a

1 hectare, 2

– m

deep pond Fresh Water Fish

and Amphibians

(Rainbow trout) Survival,

growth, and

reproduction of

individuals and

communities Survival : LC50: 14 ppb

Growth and Reproduction Risk is below the Agency’s LOCs

for the assessed endpoints.

:

NOAEC: 4.3 ppb

## 13 Appendix 2 . Table 1. Summary of Risks Identified in this Assessment from the Proposed Trifloxystrobin Uses

Ecosystem at

Risk Taxonomic group

(surrogate

species) Assessment

Endpoints a 

### Toxicity Value Used to

Evaluate Assessment

Endpoints b 

### Conclusions Regarding

Potential for the Assessment

Endpoints to be Affected by the

Proposed New Trifloxystrobin

Uses

Freshwater

invertebrates

(Water fleas) Survival

LC 50 

### : 25 ppb :

Growth and Reproduction Risk is below the Agency’s LOCs

for the assessed endpoints.

:

NOAEC: 2.8 ppb

Estuarine/ marine

fish and

amphibians

(Sheepshead

minnow) Survival Risk is below the Agency’s LOCs

for the assessed endpoints. : LC 50 

### : 78 ppb

Estuarine/ marine

invertebrates

(mysid shrimp) Survival

LC 50 

### : 8.6 ppb :

Growth and Reproduction Risk is below the Agency’s LOCs

for the assessed endpoints.

However, the chronic assessment

was based on an acute to chronic

ratio (ACR) calculated from

daphnid studies. If the ACR is

not conserved across species,

then risk could be under

- or over

estimated. :

NOAEC: 0.96 ppb (estimated

using acute to chronic ratio)

Aquatic plants and

algae

(green algae) Growth and

reproduction of

individual and

communities of

aquatic plants EC 50 

### : 37 ppb; NOAEC: 10 ppb Growth and Reproduction: Risk is below the Agency’s LOCs

for the assessed endpoints.

a 

### The assessment endpoints measure effects at the individual level; however, they provide insight into potential risks at highe r levels

(i.e., populations).

b 

### See Tables III

- 9 and III

- 10 for additional information on the toxicity studies used to derive the se values.

c 

### Four species of two families of monocots, of which one is corn; six species of at least four dicot families, of which one is soybeans.

## 14 A PPENDIX3

.

Summary of Environmental Fate Data and Model Input Parameters for Trifloxystrobin

.

Environmental Fate Characterization

The major routes of degradation and dissipation for trifloxystrobin are associated with alkaline

catalyzed hydrolysis, microbial

- mediated metabolism, photodegradation, and soil binding.

Hydrolysis of trifloxystrobin is most important under alkaline conditions. Dissipation of

trifloxystrobin will also occur in the environment by spray drift, in runoff water and eroded soil.

The major degradate in hydrolysis, metabolism and photodegradation is CGA

- 321113, the free

acid de gradation product of trifloxystrobin. (D309489). However, CGA

- 321113 is not

considered in this assessment due to the fact that the parent is more toxic than the degradate.

The decline of trifloxystrobin (half

- life < 3 day) appears to be quite rapid (D254286). The decline

pattern does not appear to follow a first order kin etics based upon visual inspection

, but tended

to follow a “hockey stick” degradation pattern (a rapid initial decline followed by a slower

decline), thus the first

- order half

- life was does not accurately describe the decline of

trifloxystrobin. Due to the rapid decline of trifloxystrobin and disappearance, residues degrade

more rapidly than estimated by first order kinetics.

Trifloxystrobin is expected to have low mobility in the environment. The range for the

Freundlich sorption coefficients (1/N) for trifloxystrobin, show there is no a correlation between

soil organic matter and K f 

## . Therefore, the lack of correlation suggests that sorptio n is controlled

by factors other than soil organic carbon content. Please see Table 1 below for values of sorption

coefficients.

Appendix 3

. Table 1. Sorption Coefficient Values for Trifloxystrobin

Parameter Trifloxystorbin

K f 

### (mL/g) 6.8 to 91.8 (Average 26.9)

1/N 0.88 to 1.30

K OC 

### (mL/g

oc 

### ) 48 to 235



## Due to the rapid degradation, low solubility (0.61 mg/L), and relatively low mobility of

trifloxystrobin, trifloxystrobin is thought to have a limited potential to contaminate water.

Likewise

, due to the low vapor pressure (2.55e

- 08 

## mm Hg @ 20 ◦ 

## C) and calculated Henry’s Law

constant (2.2e

- 08 

## atm m 3 

## mol

- 1 

## ), volatilization from both dry and moist soils and water surfaces is

not expected. Therefore, volatilization is not an important dissipation pathway.

Chemical names and chemical structure for trifloxystrobin are summarized in Figure 1.

## 15 Appendix 3. Figure 1. The co mmon name, chemical name, structure for trifloxystrobin,

and some additional information.

Common Name

Chemical Name Structure

Trifloxystrobin

(E,E)

- α

- (methoxyimino)

- 2

- [[[[1

- [3

- (trifluoromethyl) phenyl] ethylidene]

amino] methyl]

- methyl ester)

Chemical Abstracts #: 141517

- 21

- 7 N O

C H

3

O

C H

3 O O

N C H

3

C F

3



### Appendix 3. Table 2 . Summary environmental fate data and model input parameters for trifloxystrobin

which were used to estimate the EECs for the aquatic exposure assessment.

Parameter Value Modeling Input Value Source

Molecular Formula C 20 

### H 19 

### F 3 

### N 2 

### O 4 

### DP Barcode D254920

Molecular Weight 408.4 408.4 MRID 44052801

Solubility @ 20 ◦ 

### C 0.61 mg/L @ pH7 0.61 mg/L MRID 44052801

Vapor Pressure 2.55e

- 08 

### mm Hg @ 20 ◦ 

### C 2.55e

- 08 

### mm Hg @ 20 ◦ 

### C DP Barcode D254920

Henry’s Law Constant 2.2e

- 08 

### atm m 3 

### mol

- 1 

### Calculated

Hydrolysis Half

- life Stable @ pH 4

55.2 days @ pH7

0.83 days @ pH 9 Stable (0 days)

55.2 days

0.83 days MRID 44496727

Aquatic Photolysis Half

life 14.8 days @pH 5 14.8 days MRID 44496727

Photolysis on soil Half

life 2.1 days 2.1 days MRID 44496729

Aerobic Soil

Metabolism Half

- life

(T 1⁄2 

### ) 0.74, 0.41, and 0.91 days

mean = 0.69 d, std = 0.25

d 90 th 

### percent upper bound

of mean =

0.96 days MRID 44496732

MRID 44496730

MRID 44496731

EFED Guidance

Anaerobic Aquatic

Metabolism Half

- life 0.72 days 2.16 days (3 x 0.72) MRID 44496733

## 1 6 Appendix 3. Table 2 . Summary environmental fate data and model input parameters for trifloxystrobin

which were used to estimate the EECs for the aquatic exposure assessment.

Parameter Value Modeling Input Value Source

Aerobic Aquatic

Metabolism Half

- life 0.60 days (supplemental)

2 x aerobic soil

metabolism (0.96 days) 1.92 MRID 44496734

EFED Guidance

Organic Carbon

Partition Coefficient

(Koc) (mL/g) 2354, 951, 3064, 3927,

9871, 951 coefficient of

determination (r 2 

### ) = (K f 

### vs. organic matter = 0.03) Use mean K f 

### MRID 44496802

Soil Partition

Coefficient (K f 

### )

(mL/g) (K f 

### ) (texture class)

6.8 Loamy sand

7.7 Sandy loam

16.0 (Low Org Matter)

59.4 (High Org Matter

sand)

91.8 (Clay loam)

33.2 (Silt loam

- 6%OM)

Mean = 35.82; Std = 33.8 mean K f 

### = 35.82 1 

### mL/g MRID 44496802

Ground Spray Drift

Application Efficiency 0.01

0.99 0.01

0.99 EFED Input Parameter

Guidance

FEXTRA

- foliar apps PRZM Foliar Extraction 0.5 (frac/cm rain) EFED Guidance

PLDKRT

- foliar apps PRZM Decay rate of

Foliage 0 EFED Guidance

## 17 A PPENDIX4

.

Risk Characterization

Risk characterization integrates EECs and toxicity estimates to determine whether the proposed

trifloxystrobin uses pose risk to non

- target species at levels of concern to the Agency. In a

deterministic approach, a single point estima te of toxicity is divided by an exposure estimate to

calculate a risk quotient (RQ). The RQ is then compared to Agency LOCs, which serve as

criteria for categorizing potential risk to non

- target organisms. LOCs currently address the

following risk presumpt ion categories:

Risk characterization integrates EECs and toxicity estimates to determine whether the proposed

trifloxystrobin uses pose risk to non

- target species at levels of concern to the Agency. In a

deterministic approach, a single point estim ate of toxicity is divided by an exposure estimate to

calculate a risk quotient (RQ). The RQ is then compared to Agency LOCs, which serve as

criteria for categorizing potential risk to non

- target organisms. LOCs currently address the

following risk presump tion categories:

Risk presumptions for terrestrial animals

Risk Presumption Risk Quotient (RQ) Level of Concern

(LOC)

Birds

Acute Risk EEC 1 /LC 50 0.5

Acute Restricted Use EEC/LC 50 or LD 50 < 50 mg/kg 0.2

Acute Endangered Species EEC/LC 50 0.1

Chronic Risk EEC/NOAEC 1

Wild Mammals

Acute Risk EEC/LC 50 0.5

Acute Restricted Use EEC/LC 50 or LD 50 < 50 mg/kg 0.2

Acute Endangered Species EEC/LC 50 0.1

Chronic Risk EEC/NOAEC 1

1 abbreviation for Estimated Environmental Concentration (ppm) on avian/mammalian food items



### Risk presumptions for aquatic animals

Risk Presumption RQ LOC

Acute Risk EEC 1 /LC 50 or EC 50 0.5

Acute Restricted Use EEC/LC 50 or EC 50 0.1

Acute Endangered Species EEC/LC 50 or EC 50 0.05

Chronic Risk EEC/NOAEC 1

1 EEC = (ppm or ppb) in water

## 18 Risk presumptions for plants

Risk Presumption RQ LOC

Plant Inhabiting Terrestrial and Semi

- Aquatic Areas

Acute Risk EEC 1 /EC 25 1

Acute Endangered Species EEC/EC 05 or NOAEC 1

Aquatic Plants

Acute Risk EEC 2 /EC 50 1

Acute Endangered Species EEC/EC 05 or NOAEC 1

1 EEC = lbsa.i./A ; 2 EEC = (ppb or ppm) in water



## The risk estimation presents risk quotients calculated for trifloxystrobin and the LOCs (if any)

that are exceeded. Further discussion of the risk quotients and uncertainties in the assessment is

presented in the risk description for each taxa assessed.

The acute and chronic LOCs are not exceeded for either freshwater fish or invertebrates.

Minimal risk is presumed for non

- endangered and endangered freshwater fish or invertebrates

from the parent material. Based on the toxicity of CGA

- 321113 (EC50 > 95,0 00 ppb, NOAEC =

3,200 ppb for the most sensitive organism, Daphnia magna ), minimal risk to either freshwater

fish or invertebrates is also expected from the primary degradate. No additional degradate testing

is required at this time.

## 19 APPENDIX 5

.

PRZM/EXAMS output files

stored as Alfalfa

- CA.out

Chemical: trifloxystrobin

PRZM environment: CAalfalfa_WirrigOP.txt modified Thuday, 14 June 2007 at 10:20:44

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w93193.dvf mo dified Wedday, 3 July 2002 at 08:04:24

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.005626 0.00305 0.0008228 0.000288 0.000192 4.735e

- 005

1962 0.006002 0.003356 0.0008877 0.0003108 0.0002072 5.109e

- 005

1963 0.00691 2 0.004312 0.001213 0.0004249 0.0002833 6.985e

- 005

1964 0.006994 0.004125 0.001117 0.000391 0.0002607 6.41e

- 005

1965 0.002985 0.002065 0.0006323 0.0002215 0.0001476 3.641e

- 005

1966 0.01105 0.005966 0.00151 0.0005287 0.0003525 8.691e

- 005

1967 0.004768 0.002 842 0.0008296 0.0002904 0.0001936 4.774e

- 005

1968 0.00761 0.004114 0.00104 0.0003641 0.0002427 5.968e

- 005

1969 0.003656 0.002345 0.0006977 0.0002443 0.0001629 4.017e

- 005

1970 0.004061 0.002411 0.0006695 0.0002344 0.0001563 3.853e

- 005

1971 0.007107 0.00434 0.001184 0.0004145 0.0002763 6.814e

- 005

1972 0.009255 0.005054 0.001279 0.0004476 0.0002984 7.337e

- 005

1973 0.003622 0.002179 0.0005904 0.0002067 0.0001378 3.398e

- 005

1974 0.008285 0.004439 0.001108 0.0003879 0.0002586 6.376e

- 005

1975 0.00894 0.00507 0.001 314 0.0004601 0.0003068 7.564e

- 005

1976 0.005953 0.003448 0.001017 0.0003561 0.0002374 5.837e

- 005

1977 0.005545 0.002919 0.0007594 0.0002658 0.0001772 4.37e

- 005

1978 0.00852 0.00477 0.00123 0.0004306 0.0002871 7.078e

- 005

1979 0.00821 0.004401 0.001097 0.00 03839 0.000256 6.311e

- 005

1980 0.004501 0.002778 0.0008372 0.0002931 0.0001954 4.805e

- 005

1981 0.00475 0.002536 0.0006497 0.0002274 0.0001516 3.738e

- 005

1982 0.007192 0.004462 0.001239 0.0004339 0.0002893 7.133e

- 005

1983 0.01138 0.006268 0.001604 0.0005617 0.0003745 9.234e

- 005

1984 0.004499 0.002629 0.0007318 0.0002562 0.0001708 4.2e

- 005

1985 0.004824 0.002587 0.0006666 0.0002333 0.0001555 3.835e

- 005

1986 0.003561 0.00215 0.0005813 0.0002035 0.0001357 3.345e

- 005

1987 0.006459 0.003487 0.0008738 0.0003059 0. 0002039 5.029e

- 005

1988 0.004334 0.002506 0.0007049 0.0002468 0.0001645 4.045e

- 005

1989 0.01025 0.005704 0.001461 0.0005116 0.000341 8.409e

- 005

1990 0.004889 0.002898 0.0007714 0.00027 0.00018 4.439e

- 005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Da y Yearly

0.032258064516129 0.01138 0.006268 0.001604 0.0005617 0.0003745 9.234e

- 005

0.0645161290322581 0.01105 0.005966 0.00151 0.0005287 0.0003525 8.691e

- 005

0.0967741935483871 0.01025 0.005704 0.001461 0.0005116 0.000341 8.409e

- 005

0.129032258064516 0.00 9255 0.00507 0.001314 0.0004601 0.0003068 7.564e

- 005

0.161290322580645 0.00894 0.005054 0.001279 0.0004476 0.0002984 7.337e

- 005

0.193548387096774 0.00852 0.00477 0.001239 0.0004339 0.0002893 7.133e

- 005

0.225806451612903 0.008285 0.004462 0.00123 0.0004306 0.0002871 7.078e

- 005

0.258064516129032 0.00821 0.004439 0.001213 0.0004249 0.0002833 6.985e

- 005

0.290322580645161 0.00761 0.004401 0.001184 0.0004145 0.0002763 6.814e

- 005

0.32258064516129 0.007192 0.00434 0.001117 0.000391 0.0002607 6.41e

- 005

0.35483870967 7419 0.007107 0.004312 0.001108 0.0003879 0.0002586 6.376e

- 005

0.387096774193548 0.006994 0.004125 0.001097 0.0003839 0.000256 6.311e

- 005

0.419354838709677 0.006912 0.004114 0.00104 0.0003641 0.0002427 5.968e

- 005

0.451612903225806 0.006459 0.003487 0.00101 7 0.0003561 0.0002374 5.837e

- 005

0.483870967741936 0.006002 0.003448 0.0008877 0.0003108 0.0002072 5.109e

- 005

0.516129032258065 0.005953 0.003356 0.0008738 0.0003059 0.0002039 5.029e

- 005

0.548387096774194 0.005626 0.00305 0.0008372 0.0002931 0.0001954 4.80 5e

- 005

0.580645161290323 0.005545 0.002919 0.0008296 0.0002904 0.0001936 4.774e

- 005

0.612903225806452 0.004889 0.002898 0.0008228 0.000288 0.000192 4.735e

- 005

0.645161290322581 0.004824 0.002842 0.0007714 0.00027 0.00018 4.439e

- 005

## 20 0.67741935483871 0.00476 8 0.002778 0.0007594 0.0002658 0.0001772 4.37e

- 005

0.709677419354839 0.00475 0.002629 0.0007318 0.0002562 0.0001708 4.2e

- 005

0.741935483870968 0.004501 0.002587 0.0007049 0.0002468 0.0001645 4.045e

- 005

0.774193548387097 0.004499 0.002536 0.0006977 0.000244 3 0.0001629 4.017e

- 005

0.806451612903226 0.004334 0.002506 0.0006695 0.0002344 0.0001563 3.853e

- 005

0.838709677419355 0.004061 0.002411 0.0006666 0.0002333 0.0001555 3.835e

- 005

0.870967741935484 0.003656 0.002345 0.0006497 0.0002274 0.0001516 3.738e

- 005

0. 903225806451613 0.003622 0.002179 0.0006323 0.0002215 0.0001476 3.641e

- 005

0.935483870967742 0.003561 0.00215 0.0005904 0.0002067 0.0001378 3.398e

- 005

0.967741935483871 0.002985 0.002065 0.0005813 0.0002035 0.0001357 3.345e

- 005

0.1 0.0101505 0.0056406 0.0 014463 0.00050645 0.00033758 8.3245e

- 005

Average of yearly averages: 5.58266666666667e

- 005

Inputs generated by pe5.pl

- Novemeber 2006

Data used for this run:

Output File: Alfalfa

- CA

Metfile: w93193.dvf

PRZM scenario: CAalfalfa_WirrigOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E

- 8 atm

- m^3/mol

Vapor Pressure vapr 2.55E

- 8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

K oc Koc 35.82 mg/L

Photolysis half

- life kdp 2.1 days Half

- life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half

- life

Hydrolys is: pH 7 55.2 days Half

- life

Hydrolysis: pH 9 0.83 days Half

- life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of ap plication rate applied to pond

Application Date Date 16

- 6 dd/mm or dd/mmm or dd

- mm or dd

- mmm

Record 17: FILTRA

IPSCND 1

UPTKF

Record 18: PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa

- IL.out

Chemical: trifloxystrobin

PRZM environment: ILalfalfaNMC.txt modified Thuday, 14 June 2007 at 10:16:12

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w14842.dvf mo dified Wedday, 3 July 2002 at 08:04:38

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.00235 0.001408 0.0003923 0.0001376 9.176e

- 005 2.263e

- 005

1962 0.00235 0.001367 0.0003725 0.0001306 8.706e

- 005 2.147e

- 005

1963 0.00 3086 0.001736 0.0007032 0.0002467 0.0001645 4.056e

- 005

1964 0.00235 0.001334 0.0003506 0.0001229 8.191e

- 005 2.014e

- 005

1965 0.00235 0.00137 0.0003705 0.0001299 8.659e

- 005 2.135e

- 005

1966 0.00235 0.001373 0.0003722 0.0001305 8.701e

- 005 2.145e

- 005

1967 0.002 35 0.001352 0.0003674 0.0001288 8.589e

- 005 2.118e

- 005

1968 0.00235 0.001332 0.0003501 0.0001227 8.179e

- 005 2.011e

- 005

1969 0.009279 0.005798 0.0017 0.0005975 0.0003983 9.822e

- 005

## 21 1970 0.01092 0.006524 0.001811 0.0006353 0.0004235 0.0001044

1971 0.00235 0.001243 0.000307 0.0001075 7.166e

- 005 1.767e

- 005

1972 0.00235 0.001436 0.0004922 0.000173 0.0001154 2.837e

- 005

1973 0.006542 0.004158 0.001297 0.000455 0.0003033 7.479e

- 005

1974 0.00353 0.002381 0.0009841 0.0003475 0.0002316 5.712e

- 005

1975 0.00235 0.0013 49 0.0004448 0.000156 0.000104 2.565e

- 005

1976 0.00235 0.001375 0.0003733 0.0001309 8.726e

- 005 2.146e

- 005

1977 0.00235 0.001399 0.0003877 0.000136 9.067e

- 005 2.236e

- 005

1978 0.00235 0.001359 0.0003644 0.0001277 8.516e

- 005 2.1e

- 005

1979 0.00235 0.001374 0.0 003765 0.000132 8.802e

- 005 2.17e

- 005

1980 0.1706 0.1114 0.03312 0.01162 0.007749 0.001905

1981 0.00235 0.001313 0.0003407 0.0001194 7.957e

- 005 1.962e

- 005

1982 0.00235 0.001459 0.0004247 0.0001492 9.947e

- 005 2.453e

- 005

1983 0.00235 0.00167 0.0004846 0.00016 98 0.0001132 2.792e

- 005

1984 0.00235 0.001292 0.000347 0.0001216 8.105e

- 005 1.993e

- 005

1985 0.00235 0.001516 0.0004725 0.000166 0.0001107 2.728e

- 005

1986 0.003084 0.001891 0.0007977 0.0002802 0.0001868 4.607e

- 005

1987 0.05267 0.02904 0.007524 0.002636 0.00 1757 0.0004333

1988 0.00235 0.001299 0.0003329 0.0001166 7.774e

- 005 1.912e

- 005

1989 0.00235 0.00139 0.0003816 0.0001338 8.923e

- 005 2.2e

- 005

1990 0.00235 0.001375 0.0003759 0.0001318 8.786e

- 005 2.167e

- 005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Da y Yearly

0.032258064516129 0.1706 0.1114 0.03312 0.01162 0.007749 0.001905

0.0645161290322581 0.05267 0.02904 0.007524 0.002636 0.001757 0.0004333

0.0967741935483871 0.01092 0.006524 0.001811 0.0006353 0.0004235 0.0001044

0.129032258064516 0.009279 0.00579 8 0.0017 0.0005975 0.0003983 9.822e

- 005

0.161290322580645 0.006542 0.004158 0.001297 0.000455 0.0003033 7.479e

- 005

0.193548387096774 0.00353 0.002381 0.0009841 0.0003475 0.0002316 5.712e

- 005

0.225806451612903 0.003086 0.001891 0.0007977 0.0002802 0.0001868 4.607e

- 005

0.258064516129032 0.003084 0.001736 0.0007032 0.0002467 0.0001645 4.056e

- 005

0.290322580645161 0.00235 0.00167 0.0004922 0.000173 0.0001154 2.837e

- 005

0.32258064516129 0.00235 0.001516 0.0004846 0.0001698 0.0001132 2.792e

- 005

0.354838709677419 0.00235 0.001459 0.0004725 0.000166 0.0001107 2.728e

- 005

0.387096774193548 0.00235 0.001436 0.0004448 0.000156 0.000104 2.565e

- 005

0.419354838709677 0.00235 0.001408 0.0004247 0.0001492 9.947e

- 005 2.453e

- 005

0.451612903225806 0.00235 0.001399 0.0003923 0.0 001376 9.176e

- 005 2.263e

- 005

0.483870967741936 0.00235 0.00139 0.0003877 0.000136 9.067e

- 005 2.236e

- 005

0.516129032258065 0.00235 0.001375 0.0003816 0.0001338 8.923e

- 005 2.2e

- 005

0.548387096774194 0.00235 0.001375 0.0003765 0.000132 8.802e

- 005 2.17e

- 005

0. 580645161290323 0.00235 0.001374 0.0003759 0.0001318 8.786e

- 005 2.167e

- 005

0.612903225806452 0.00235 0.001373 0.0003733 0.0001309 8.726e

- 005 2.147e

- 005

0.645161290322581 0.00235 0.00137 0.0003725 0.0001306 8.706e

- 005 2.146e

- 005

0.67741935483871 0.00235 0.0 01367 0.0003722 0.0001305 8.701e

- 005 2.145e

- 005

0.709677419354839 0.00235 0.001359 0.0003705 0.0001299 8.659e

- 005 2.135e

- 005

0.741935483870968 0.00235 0.001352 0.0003674 0.0001288 8.589e

- 005 2.118e

- 005

0.774193548387097 0.00235 0.001349 0.0003644 0.0001277 8.516e

- 005 2.1e

- 005

0.806451612903226 0.00235 0.001334 0.0003506 0.0001229 8.191e

- 005 2.014e

- 005

0.838709677419355 0.00235 0.001332 0.0003501 0.0001227 8.179e

- 005 2.011e

- 005

0.870967741935484 0.00235 0.001313 0.000347 0.0001216 8.105e

- 005 1.993e

- 005

0.903 225806451613 0.00235 0.001299 0.0003407 0.0001194 7.957e

- 005 1.962e

- 005

0.935483870967742 0.00235 0.001292 0.0003329 0.0001166 7.774e

- 005 1.912e

- 005

0.967741935483871 0.00235 0.001243 0.000307 0.0001075 7.166e

- 005 1.767e

- 005

0.1 0.0107559 0.0064514 0.0017 999 0.00063152 0.00042098 0.000103782

Average of yearly averages: 0.000108269

Inputs generated by pe5.pl

- Novemeber 2006

Data used for this run:

Output File: Alfalfa

- IL

Metfile: w14842.dvf

PRZM scenario: ILalfalfaNMC.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

## 22 Henry's Law Const. henry 2.2E

- 8 atm

- m^3/mol

Vapor Pressure vapr 2.55E

- 8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half

- life kdp 2.1 days Half

- life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half

- life

Hydrolysis: pH 7 55.2 days Half

- life

Hydrolysis: pH 9 0.83 days Half

- life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate app lied to pond

Application Date Date 1

- 6 dd/mm or dd/mmm or dd

- mm or dd

- mmm

Record 17: FILTRA

IPSCND 1

UPTKF

Record 18: PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa

- MN.out

Chemical: trifloxystrobin

PRZM environment: MNalfalfaOP.txt modified Thuday, 14 June 2007 at 10:21:44

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w14914.dvf modified Wedday, 3 J uly 2002 at 08:05:52

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.00235 0.00141 0.0003937 0.0001381 9.208e

- 005 2.271e

- 005

1962 0.00235 0.001473 0.0004527 0.0001593 0.0001062 2.618e

- 005

1963 0.00235 0.001417 0.00039 76 0.0001395 9.303e

- 005 2.294e

- 005

1964 0.00235 0.001455 0.0004215 0.0001481 9.872e

- 005 2.428e

- 005

1965 0.003579 0.002452 0.0008084 0.000285 0.00019 4.685e

- 005

1966 0.00235 0.001479 0.0004437 0.000156 0.000104 2.565e

- 005

1967 0.00235 0.001554 0.0005125 0.0 001811 0.0001207 2.976e

- 005

1968 0.00235 0.001528 0.0004861 0.0001714 0.0001143 2.81e

- 005

1969 0.00235 0.001647 0.0005764 0.0002052 0.0001368 3.374e

- 005

1970 0.00235 0.001429 0.0004051 0.0001422 9.481e

- 005 2.338e

- 005

1971 0.00235 0.001486 0.000449 0.0001577 0.0001051 2.592e

- 005

1972 0.00235 0.001431 0.0004063 0.0001426 9.51e

- 005 2.338e

- 005

1973 0.00235 0.001499 0.0004516 0.0001589 0.0001059 2.612e

- 005

1974 0.00235 0.001495 0.0004487 0.0001579 0.0001052 2.595e

- 005

1975 0.00235 0.001499 0.0004519 0.00 0159 0.000106 2.614e

- 005

1976 0.00235 0.001429 0.000405 0.0001422 9.479e

- 005 2.331e

- 005

1977 0.00235 0.001475 0.0004354 0.0001531 0.000102 2.516e

- 005

1978 0.00235 0.001502 0.0004544 0.00016 0.0001066 2.629e

- 005

1979 0.00235 0.00148 0.0004385 0.0001542 0.00 01028 2.534e

- 005

1980 0.00235 0.00149 0.0004453 0.0001567 0.0001044 2.568e

- 005

1981 0.00235 0.001549 0.0004899 0.0001729 0.0001152 2.842e

- 005

1982 0.00235 0.001606 0.0005378 0.0001906 0.0001271 3.134e

- 005

1983 0.00235 0.001489 0.0004445 0.0001564 0.0001042 2.57e

- 005

1984 0.00235 0.001489 0.0005778 0.0002041 0.0001361 3.346e

- 005

1985 0.00235 0.0016 0.000533 0.0001889 0.0001259 3.104e

- 005

1986 0.00235 0.001446 0.0004166 0.0001463 9.754e

- 005 2.405e

- 005

1987 0.00235 0.001395 0.0003843 0.0001348 8.987e

- 005 2.216 e

- 005

1988 0.00235 0.001287 0.0003272 0.0001146 7.64e

- 005 1.879e

- 005

1989 0.00235 0.001507 0.0004578 0.0001611 0.0001074 2.649e

- 005

1990 0.03843 0.02409 0.007089 0.002492 0.001661 0.0004097

Sorted results

## 23 Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032 258064516129 0.03843 0.02409 0.007089 0.002492 0.001661 0.0004097

0.0645161290322581 0.003579 0.002452 0.0008084 0.000285 0.00019 4.685e

- 005

0.0967741935483871 0.00235 0.001647 0.0005778 0.0002052 0.0001368 3.374e

- 005

0.129032258064516 0.00235 0.001606 0.0005764 0.0002041 0.0001361 3.346e

- 005

0.161290322580645 0.00235 0.0016 0.0005378 0.0001906 0.0001271 3.134e

- 005

0.193548387096774 0.00235 0.001554 0.000533 0.0001889 0.0001259 3.104e

- 005

0.225806451612903 0.00235 0.001549 0.0005125 0.0001811 0.0001207 2 .976e

- 005

0.258064516129032 0.00235 0.001528 0.0004899 0.0001729 0.0001152 2.842e

- 005

0.290322580645161 0.00235 0.001507 0.0004861 0.0001714 0.0001143 2.81e

- 005

0.32258064516129 0.00235 0.001502 0.0004578 0.0001611 0.0001074 2.649e

- 005

0.354838709677419 0. 00235 0.001499 0.0004544 0.00016 0.0001066 2.629e

- 005

0.387096774193548 0.00235 0.001499 0.0004527 0.0001593 0.0001062 2.618e

- 005

0.419354838709677 0.00235 0.001495 0.0004519 0.000159 0.000106 2.614e

- 005

0.451612903225806 0.00235 0.00149 0.0004516 0.000158 9 0.0001059 2.612e

- 005

0.483870967741936 0.00235 0.001489 0.000449 0.0001579 0.0001052 2.595e

- 005

0.516129032258065 0.00235 0.001489 0.0004487 0.0001577 0.0001051 2.592e

- 005

0.548387096774194 0.00235 0.001486 0.0004453 0.0001567 0.0001044 2.57e

- 005

0.58064 5161290323 0.00235 0.00148 0.0004445 0.0001564 0.0001042 2.568e

- 005

0.612903225806452 0.00235 0.001479 0.0004437 0.000156 0.000104 2.565e

- 005

0.645161290322581 0.00235 0.001475 0.0004385 0.0001542 0.0001028 2.534e

- 005

0.67741935483871 0.00235 0.001473 0.00 04354 0.0001531 0.000102 2.516e

- 005

0.709677419354839 0.00235 0.001455 0.0004215 0.0001481 9.872e

- 005 2.428e

- 005

0.741935483870968 0.00235 0.001446 0.0004166 0.0001463 9.754e

- 005 2.405e

- 005

0.774193548387097 0.00235 0.001431 0.0004063 0.0001426 9.51e

- 005 2 .338e

- 005

0.806451612903226 0.00235 0.001429 0.0004051 0.0001422 9.481e

- 005 2.338e

- 005

0.838709677419355 0.00235 0.001429 0.000405 0.0001422 9.479e

- 005 2.331e

- 005

0.870967741935484 0.00235 0.001417 0.0003976 0.0001395 9.303e

- 005 2.294e

- 005

0.90322580645161 3 0.00235 0.00141 0.0003937 0.0001381 9.208e

- 005 2.271e

- 005

0.935483870967742 0.00235 0.001395 0.0003843 0.0001348 8.987e

- 005 2.216e

- 005

0.967741935483871 0.00235 0.001287 0.0003272 0.0001146 7.64e

- 005 1.879e

- 005

0.1 0.00235 0.0016429 0.00057766 0.0002050 9 0.00013673 3.3712e

- 005

Average of yearly averages: 3.9601e

- 005

Inputs generated by pe5.pl

- Novemeber 2006

Data used for this run:

Output File: Alfalfa

- MN

Metfile: w14914.dvf

PRZM scenario: MNalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E

- 8 atm

- m^3/mol

Vapor Pressure vapr 2.55E

- 8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis h alf

- life kdp 2.1 days Half

- life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half

- life

Hydrolysis: pH 7 55.2 days Half

- life

H ydrolysis: pH 9 0.83 days Half

- life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 1

- 6 dd/mm or dd/mmm or dd

- mm or dd

- mmm

Record 17: FILTRA

IPSCND 1

UPTKF

Record 18: PLVKRT

PLDKRT

## 24 FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa

- NC.out

Chemical: trifloxystrobin

PRZM environment: NCalfalfaOP.txt modified Thuday, 14 June 2007 at 10:21:52

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w03812.dvf modified Wedday, 3 July 2002 at 0 8:05:50

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1965 0.00235 0.001681 0.0006107 0.0002185 0.0001457 3.593e

- 005

1966 0.00235 0.001769 0.0007132 0.00026 0.0001733 4.274e

- 005

1967 0.00235 0.001659 0.0005885 0.0002101 0. 0001401 3.453e

- 005

1968 0.003244 0.002362 0.001133 0.0004111 0.000274 6.739e

- 005

1969 0.00235 0.001686 0.0006165 0.0002209 0.0001472 3.63e

- 005

1970 0.03884 0.02858 0.01033 0.003693 0.002462 0.0006071

1971 0.00235 0.001698 0.0006293 0.000226 0.0001506 3.714e

- 005

1972 0.00235 0.001701 0.000633 0.0002275 0.0001516 3.729e

- 005

1973 0.00235 0.001748 0.0007794 0.0002847 0.0001898 4.68e

- 005

1974 0.01729 0.01401 0.005535 0.002012 0.001341 0.0003307

1975 0.00235 0.001726 0.0006606 0.0002383 0.0001588 3.917e

- 005

1976 0.00235 0.001701 0.0006326 0.0002273 0.0001516 3.727e

- 005

1977 0.01023 0.007189 0.002767 0.0009974 0.0006649 0.000164

1978 0.00235 0.001672 0.0006012 0.000215 0.0001433 3.534e

- 005

1979 0.01676 0.01273 0.004851 0.001754 0.001169 0.0002883

1980 0.00235 0.001674 0.0006364 0.0002282 0.0001522 3.742e

- 005

1981 0.00235 0.001618 0.0005485 0.0001948 0.0001299 3.202e

- 005

1982 0.00235 0.001746 0.000685 0.000248 0.0001653 4.077e

- 005

1983 0.0361 0.02762 0.01155 0.004247 0.002831 0.0006982

1984 0.004481 0.003378 0.0 0161 0.0005957 0.0003972 9.766e

- 005

1985 0.00235 0.00168 0.00079 0.0002843 0.0001896 4.674e

- 005

1986 0.00235 0.001678 0.0006071 0.0002172 0.0001448 3.571e

- 005

1987 0.00235 0.001749 0.000777 0.000283 0.0001887 4.652e

- 005

1988 0.004834 0.003532 0.001513 0.00 0549 0.000366 9.001e

- 005

1989 0.00235 0.001792 0.0007431 0.0002689 0.0001793 4.42e

- 005

1990 0.00235 0.001726 0.0006608 0.0002384 0.0001589 3.919e

- 005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.037037037037037 0.03884 0.02858 0.01155 0.0 04247 0.002831 0.0006982

0.0740740740740741 0.0361 0.02762 0.01033 0.003693 0.002462 0.0006071

0.111111111111111 0.01729 0.01401 0.005535 0.002012 0.001341 0.0003307

0.148148148148148 0.01676 0.01273 0.004851 0.001754 0.001169 0.0002883

0.185185185185185 0 .01023 0.007189 0.002767 0.0009974 0.0006649 0.000164

0.222222222222222 0.004834 0.003532 0.00161 0.0005957 0.0003972 9.766e

- 005

0.259259259259259 0.004481 0.003378 0.001513 0.000549 0.000366 9.001e

- 005

0.296296296296296 0.003244 0.002362 0.001133 0.0004111 0.000274 6.739e

- 005

0.333333333333333 0.00235 0.001792 0.00079 0.0002847 0.0001898 4.68e

- 005

0.37037037037037 0.00235 0.001769 0.0007794 0.0002843 0.0001896 4.674e

- 005

0.407407407407407 0.00235 0.001749 0.000777 0.000283 0.0001887 4.652e

- 005

0.44 4444444444444 0.00235 0.001748 0.0007431 0.0002689 0.0001793 4.42e

- 005

0.481481481481481 0.00235 0.001746 0.0007132 0.00026 0.0001733 4.274e

- 005

0.518518518518518 0.00235 0.001726 0.000685 0.000248 0.0001653 4.077e

- 005

0.555555555555556 0.00235 0.001726 0. 0006608 0.0002384 0.0001589 3.919e

- 005

0.592592592592593 0.00235 0.001701 0.0006606 0.0002383 0.0001588 3.917e

- 005

0.62962962962963 0.00235 0.001701 0.0006364 0.0002282 0.0001522 3.742e

- 005

0.666666666666667 0.00235 0.001698 0.000633 0.0002275 0.0001516 3. 729e

- 005

0.703703703703704 0.00235 0.001686 0.0006326 0.0002273 0.0001516 3.727e

- 005

0.740740740740741 0.00235 0.001681 0.0006293 0.000226 0.0001506 3.714e

- 005

0.777777777777778 0.00235 0.00168 0.0006165 0.0002209 0.0001472 3.63e

- 005

0.814814814814815 0.00 235 0.001678 0.0006107 0.0002185 0.0001457 3.593e

- 005

0.851851851851852 0.00235 0.001674 0.0006071 0.0002172 0.0001448 3.571e

- 005

0.888888888888889 0.00235 0.001672 0.0006012 0.000215 0.0001433 3.534e

- 005

0.925925925925926 0.00235 0.001659 0.0005885 0.0002 101 0.0001401 3.453e

- 005

0.962962962962963 0.00235 0.001618 0.0005485 0.0001948 0.0001299 3.202e

- 005

## 25 0.1 0.022933 0.018093 0.0069735 0.0025163 0.0016773 0.00041362

Average of yearly averages: 0.000117247692307692

Inputs generated by pe5.pl

- Novemeb er 2006

Data used for this run:

Output File: Alfalfa

- NC

Metfile: w03812.dvf

PRZM scenario: NCalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E

- 8 atm

- m^3/mol

Vapor Pressure vapr 2.55E

- 8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half

- life kdp 2.1 days Half

- life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half

- life

Hydrolysis: pH 7 55.2 days Half

- life

Hydrolysis: pH 9 0.83 days Half

- life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Ap plication Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 1

- 4 dd/mm or dd/mmm or dd

- mm or dd

- mmm

Record 17: FILTRA

IPSCND 1

UPTKF

Record 18: P LVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa

- PA.out

Chemical: trifloxystrobin

PRZM environment: PAalfalfaOP.txt modified Thuday, 14 June 2007 at 10:23:40

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w14751.dvf modified Wedday, 3 July 2002 at 08:06:14

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.01274 0.009912 0 .004262 0.001538 0.001026 0.0002529

1962 0.00235 0.001761 0.0006949 0.0002472 0.0001648 4.063e

- 005

1963 0.00235 0.001714 0.0006437 0.0002293 0.0001528 3.769e

- 005

1964 0.00235 0.001796 0.0007551 0.0002689 0.0001793 4.408e

- 005

1965 0.00235 0.001794 0.0007337 0.0002606 0.0001737 4.284e

- 005

1966 0.00235 0.001805 0.0007534 0.0002698 0.0001799 4.435e

- 005

1967 0.00235 0.001751 0.0006892 0.0002489 0.0001659 4.092e

- 005

1968 0.00235 0.001723 0.0006938 0.0002497 0.0001665 4.094e

- 005

1969 0.00235 0.001721 0.0006498 0.0 002308 0.0001539 3.794e

- 005

1970 0.00235 0.001773 0.0007439 0.0002646 0.0001764 4.35e

- 005

1971 0.00235 0.001783 0.000726 0.0002603 0.0001735 4.279e

- 005

1972 0.0143 0.01097 0.004558 0.00163 0.001087 0.0002673

1973 0.00235 0.001779 0.0007277 0.0002627 0.0001751 4.318e

- 005

1974 0.00235 0.001678 0.0006046 0.0002145 0.000143 3.526e

- 005

1975 0.00235 0.001834 0.0008092 0.0002895 0.000193 4.759e

- 005

1976 0.00235 0.00171 0.00064 0.0002286 0.0001524 3.747e

- 005

1977 0.00235 0.001726 0.0006725 0.000239 0.0001593 3.928e

- 005

1978 0.00235 0.001876 0.0008403 0.0003008 0.0002006 4.945e

- 005

## 26 1979 0.00235 0.001774 0.0007128 0.0002545 0.0001697 4.184e

- 005

1980 0.00235 0.001724 0.000654 0.0002322 0.0001548 3.807e

- 005

1981 0.00235 0.001701 0.0006289 0.0002237 0.0001492 3.678 e

- 005

1982 0.00235 0.00183 0.0007866 0.000282 0.000188 4.636e

- 005

1983 0.00235 0.001806 0.0008124 0.0002943 0.0001962 4.837e

- 005

1984 0.00235 0.001826 0.0007829 0.0002824 0.0001882 4.629e

- 005

1985 0.00235 0.001736 0.0006681 0.0002384 0.0001589 3.919e

- 005

1 986 0.1051 0.08226 0.03301 0.01176 0.00784 0.001933

1987 0.00235 0.001767 0.0007031 0.0002506 0.0001671 4.119e

- 005

1988 0.00235 0.001791 0.0007337 0.0002623 0.0001748 4.299e

- 005

1989 0.00235 0.001778 0.0007193 0.000258 0.000172 4.241e

- 005

1990 0.00235 0.00 1737 0.0006709 0.0002401 0.0001601 3.947e

- 005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.1051 0.08226 0.03301 0.01176 0.00784 0.001933

0.0645161290322581 0.0143 0.01097 0.004558 0.00163 0.001087 0.0002673

0.09677419354 83871 0.01274 0.009912 0.004262 0.001538 0.001026 0.0002529

0.129032258064516 0.00235 0.001876 0.0008403 0.0003008 0.0002006 4.945e

- 005

0.161290322580645 0.00235 0.001834 0.0008124 0.0002943 0.0001962 4.837e

- 005

0.193548387096774 0.00235 0.00183 0.0008092 0.0002895 0.000193 4.759e

- 005

0.225806451612903 0.00235 0.001826 0.0007866 0.0002824 0.0001882 4.636e

- 005

0.258064516129032 0.00235 0.001806 0.0007829 0.000282 0.000188 4.629e

- 005

0.290322580645161 0.00235 0.001805 0.0007551 0.0002698 0.0001799 4.435e

- 005

0.32258064516129 0.00235 0.001796 0.0007534 0.0002689 0.0001793 4.408e

- 005

0.354838709677419 0.00235 0.001794 0.0007439 0.0002646 0.0001764 4.35e

- 005

0.387096774193548 0.00235 0.001791 0.0007337 0.0002627 0.0001751 4.318e

- 005

0.419354838709677 0.00235 0.00 1783 0.0007337 0.0002623 0.0001748 4.299e

- 005

0.451612903225806 0.00235 0.001779 0.0007277 0.0002606 0.0001737 4.284e

- 005

0.483870967741936 0.00235 0.001778 0.000726 0.0002603 0.0001735 4.279e

- 005

0.516129032258065 0.00235 0.001774 0.0007193 0.000258 0.000 172 4.241e

- 005

0.548387096774194 0.00235 0.001773 0.0007128 0.0002545 0.0001697 4.184e

- 005

0.580645161290323 0.00235 0.001767 0.0007031 0.0002506 0.0001671 4.119e

- 005

0.612903225806452 0.00235 0.001761 0.0006949 0.0002497 0.0001665 4.094e

- 005

0.64516129032 2581 0.00235 0.001751 0.0006938 0.0002489 0.0001659 4.092e

- 005

0.67741935483871 0.00235 0.001737 0.0006892 0.0002472 0.0001648 4.063e

- 005

0.709677419354839 0.00235 0.001736 0.0006725 0.0002401 0.0001601 3.947e

- 005

0.741935483870968 0.00235 0.001726 0.00067 09 0.000239 0.0001593 3.928e

- 005

0.774193548387097 0.00235 0.001724 0.0006681 0.0002384 0.0001589 3.919e

- 005

0.806451612903226 0.00235 0.001723 0.000654 0.0002322 0.0001548 3.807e

- 005

0.838709677419355 0.00235 0.001721 0.0006498 0.0002308 0.0001539 3.794e

- 005

0.870967741935484 0.00235 0.001714 0.0006437 0.0002293 0.0001528 3.769e

- 005

0.903225806451613 0.00235 0.00171 0.00064 0.0002286 0.0001524 3.747e

- 005

0.935483870967742 0.00235 0.001701 0.0006289 0.0002237 0.0001492 3.678e

- 005

0.967741935483871 0.00235 0 .001678 0.0006046 0.0002145 0.000143 3.526e

- 005

0.1 0.011701 0.0091084 0.00391983 0.00141428 0.00094346 0.000232555

Average of yearly averages: 0.000119469

Inputs generated by pe5.pl

- Novemeber 2006

Data used for this run:

Output File: Alfalfa

- PA

Metfile: w14751.dvf

PRZM scenario: PAalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E

- 8 atm

- m^3/mol

Vapor Pressure vapr 2.55E

- 8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half

- life kdp 2.1 days Half

- life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

## 27 Hydrolysis: pH 5 500 days Half

- life

Hydrolysis: pH 7 55.2 days Half

- life

Hydrolysis: pH 9 0.83 days Half

- life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 16

- 4 dd/mm or dd/mmm or dd

- mm or dd

- mmm

Record 17: FILTRA

IPSCND 1

UPTKF

Record 18: PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa

- TX.out

Chemical: trifloxystrobin

PRZM environment: TXalfalfaOP.txt modified Thuday, 14 June 2007 at 10:23:54

EXAMS environment: pond298.exv modifie d Tueday, 26 August 2008 at 05:14:07

Metfile: w13958.dvf modified Wedday, 3 July 2002 at 08:06:24

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.00235 0.001216 0.0003101 0.0001086 7.243e

- 005 1.786e

- 005

1962 0.00235 0 .001184 0.0003455 0.000121 8.066e

- 005 1.989e

- 005

1963 0.00235 0.001137 0.0002637 9.231e

- 005 6.154e

- 005 1.517e

- 005

1964 0.00235 0.00119 0.000289 0.0001012 6.745e

- 005 1.659e

- 005

1965 0.00235 0.001168 0.0002754 9.641e

- 005 6.427e

- 005 1.585e

- 005

1966 0.00235 0. 00123 0.0003558 0.0001247 8.312e

- 005 2.05e

- 005

1967 0.04422 0.02639 0.007109 0.002491 0.001661 0.0004095

1968 0.01428 0.007978 0.002227 0.0007807 0.0005205 0.000128

1969 0.00235 0.001558 0.0004113 0.000144 9.6e

- 005 2.367e

- 005

1970 0.07344 0.03791 0.009187 0.003216 0.002144 0.0005287

1971 0.00235 0.001202 0.0002925 0.0001024 6.827e

- 005 1.683e

- 005

1972 0.00235 0.001129 0.0002607 9.125e

- 005 6.083e

- 005 1.496e

- 005

1973 0.00235 0.001195 0.0002979 0.0001043 6.953e

- 005 1.714e

- 005

1974 0.00235 0.001364 0.0003672 0.0 001287 8.582e

- 005 2.116e

- 005

1975 0.00235 0.001292 0.0003297 0.0001155 7.699e

- 005 1.898e

- 005

1976 0.07821 0.04101 0.01015 0.003555 0.00237 0.0005828

1977 0.00235 0.001102 0.0002524 8.837e

- 005 5.891e

- 005 1.453e

- 005

1978 0.0291 0.01505 0.003662 0.001282 0.00 08548 0.0002108

1979 0.00235 0.001245 0.0003077 0.0001078 7.184e

- 005 1.771e

- 005

1980 0.00235 0.001137 0.0004375 0.0001532 0.0001021 2.512e

- 005

1981 0.04155 0.02092 0.005132 0.001797 0.001198 0.0002954

1982 0.003986 0.002147 0.0005938 0.0002079 0.0001386 3. 417e

- 005

1983 0.00235 0.001229 0.0003009 0.0001054 7.023e

- 005 1.732e

- 005

1984 0.006377 0.003469 0.001039 0.0003641 0.0002427 5.968e

- 005

1985 0.00235 0.001194 0.0003121 0.0001093 7.286e

- 005 1.796e

- 005

1986 0.0315 0.01542 0.003895 0.001364 0.0009091 0.000224 2

1987 0.00235 0.001199 0.0003288 0.0001152 7.682e

- 005 1.894e

- 005

1988 0.00235 0.001134 0.0002625 9.189e

- 005 6.126e

- 005 1.506e

- 005

1989 0.00235 0.001197 0.0002874 0.0001006 6.707e

- 005 1.654e

- 005

1990 0.00235 0.001164 0.0002757 9.651e

- 005 6.434e

- 005 1.587e

- 005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.07821 0.04101 0.01015 0.003555 0.00237 0.0005828

0.0645161290322581 0.07344 0.03791 0.009187 0.003216 0.002144 0.0005287

0.0967741935483871 0.04422 0.02639 0.007109 0.0024 91 0.001661 0.0004095

0.129032258064516 0.04155 0.02092 0.005132 0.001797 0.001198 0.0002954

0.161290322580645 0.0315 0.01542 0.003895 0.001364 0.0009091 0.0002242

0.193548387096774 0.0291 0.01505 0.003662 0.001282 0.0008548 0.0002108

0.225806451612903 0.01428 0.007978 0.002227 0.0007807 0.0005205 0.000128

0.258064516129032 0.006377 0.003469 0.001039 0.0003641 0.0002427 5.968e

- 005

## 28 0.290322580645161 0.003986 0.002147 0.0005938 0.0002079 0.0001386 3.417e

- 005

0.32258064516129 0.00235 0.001558 0.0004375 0.00 01532 0.0001021 2.512e

- 005

0.354838709677419 0.00235 0.001364 0.0004113 0.000144 9.6e

- 005 2.367e

- 005

0.387096774193548 0.00235 0.001292 0.0003672 0.0001287 8.582e

- 005 2.116e

- 005

0.419354838709677 0.00235 0.001245 0.0003558 0.0001247 8.312e

- 005 2.05e

- 005

0. 451612903225806 0.00235 0.00123 0.0003455 0.000121 8.066e

- 005 1.989e

- 005

0.483870967741936 0.00235 0.001229 0.0003297 0.0001155 7.699e

- 005 1.898e

- 005

0.516129032258065 0.00235 0.001216 0.0003288 0.0001152 7.682e

- 005 1.894e

- 005

0.548387096774194 0.00235 0.0 01202 0.0003121 0.0001093 7.286e

- 005 1.796e

- 005

0.580645161290323 0.00235 0.001199 0.0003101 0.0001086 7.243e

- 005 1.786e

- 005

0.612903225806452 0.00235 0.001197 0.0003077 0.0001078 7.184e

- 005 1.771e

- 005

0.645161290322581 0.00235 0.001195 0.0003009 0.0001054 7.023e

- 005 1.732e

- 005

0.67741935483871 0.00235 0.001194 0.0002979 0.0001043 6.953e

- 005 1.714e

- 005

0.709677419354839 0.00235 0.00119 0.0002925 0.0001024 6.827e

- 005 1.683e

- 005

0.741935483870968 0.00235 0.001184 0.000289 0.0001012 6.745e

- 005 1.659e

- 005

0.774 193548387097 0.00235 0.001168 0.0002874 0.0001006 6.707e

- 005 1.654e

- 005

0.806451612903226 0.00235 0.001164 0.0002757 9.651e

- 005 6.434e

- 005 1.587e

- 005

0.838709677419355 0.00235 0.001137 0.0002754 9.641e

- 005 6.427e

- 005 1.585e

- 005

0.870967741935484 0.00235 0. 001137 0.0002637 9.231e

- 005 6.154e

- 005 1.517e

- 005

0.903225806451613 0.00235 0.001134 0.0002625 9.189e

- 005 6.126e

- 005 1.506e

- 005

0.935483870967742 0.00235 0.001129 0.0002607 9.125e

- 005 6.083e

- 005 1.496e

- 005

0.967741935483871 0.00235 0.001102 0.0002524 8.837 e

- 005 5.891e

- 005 1.453e

- 005

0.1 0.043953 0.025843 0.0069113 0.0024216 0.0016147 0.00039809

Average of yearly averages: 9.503e

- 005

Inputs generated by pe5.pl

- Novemeber 2006

Data used for this run:

Output File: Alfalfa

- TX

Metfile: w13958.dvf

PRZM scenario: TXalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E

- 8 atm

- m^3/mol

Vapor Pressure vapr 2.55E

- 8 torr

Solubi lity sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half

- life kdp 2.1 days Half

- life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half

- life

Hydrolysis: pH 7 55.2 days Half

- life

Hydrolysis: pH 9 0.83 days Half

- life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spra y Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 1

- 9 dd/mm or dd/mmm or dd

- mm or dd

- mmm

Record 17: FILTRA

IPSCND 1

UPTKF

Record 18: PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

## 29 A PPENDIX 6.

Risk Quotient Tables for Aquatic and Terrestrial Animals and Plants

Freshwater Fish and Invertebrates, acute r isk

Table 6

- 1 . Acute RQs for Trifloxystrobin for Freshwater Fish and

Invertebrates from use on alfalfa

Scenario Peak EEC

( μg/L) Fish RQ 1



### (EEC/LC50) Invertebrate RQ 2



### (EEC/EC50)

Caalfalfa 0.0102 0.001 <0.001

ILalfalfaNMC 0.0108 0.001 <0.001

Mnalfalfa 0.0024 <0.001 <0.001

Ncalfalfa 0.0229 0.002 0.001

Paalfalfa 0.0117 0.001 <0.001

Txalfalfa 0.0440 0.003 0.002

1 

### based on the rainbow trout EC 50 

### of 14 ppb

2 

### based on the water flea EC 50 

### of 25 ppb

* 

### Exceeds the LOC for Acute endangered species (RQ ≥ 0.05)



## Freshwater Fish and Invertebrates, chronic risk

Table 6

- 2 . Chronic RQs for Trifloxystrobin for Freshwater Fish and Invertebrates from use on alfalfa

Scenario 60

- day Avg

EEC

( μg/L) 21

- day

Avg EEC

( μg/L) Fish RQ 1



### (EEC/NOEC) Invertebrate RQ 2 

### (EEC/NOEC)

Caalfalfa 0.0005 0.0014 <0.001 0.001

ILalfalfaNMC 0.0006 0.0018 <0.001 0.001

Mnalfalfa 0.0002 0.0006 <0.001 <0.001

Ncalfalfa 0.0025 0.0070 0.001 0.002

Paalfalfa 0.0014 0.0039 <0.001 0.001

Txalfalfa 0.0024 0.0069 0.001 0.002

1 

### based on the rainbow trout early life

- stage NOAEC of 4.3 ppb

2 

### based on the water flea life stage NOAEC of 2.8 ppb



## Marine/Estuarine Fish and Invertebrates Acute and Chronic Risk

Table 6

- 3 . Acute and Chronic RQs for Trifloxystrobin for Estuarine/Marine Organisms from useon

alfalfa

EEC

Modeled Peak

EEC

(μg/L) 60

- day

Avg

EEC

ppb 21

- day

Avg EEC

ppb 

### Acute Fish

RQ 1



### (EEC/LC 50 

### ) Acute

Invertebrate

RQ 2



### (EEC/LC 50 

### ) Chronic Fish

RQ

(EEC/NOEC) Chronic

Invertebrate

RQ 3



### (EEC/NOEC)

Caalfalfa 0.0102 <0.001 0.001 No Data 0.002

ILalfalfaNMC 0.0108 0.0005 0.0014 <0.001 0.001 No Data 0.002

Mnalfalfa 0.0024 0.0006 0.0018 <0.001 <0.001 No Data 0.001

Ncalfalfa 0.0229 0.0002 0.0006 <0.001 0.003 No Data 0.007

Paalfalfa 0.0117 0.0025 0.0070 <0.001 0.001 No Data 0.004

Txalfalfa 0.0440 0.0014 0.0039 0.001 0.005 No Data 0.007

1 

### based on the sheepshead minnow LC50 of 78 ppb

2 

### based on the mysid shrimp LC50 of 8.6 ppb

## 30 3 The estuarine/marine chronic NOAEC was estimated using acute to chronic ratios (ACRs) derived from daphnid acute (EC50=25 ppb) and

chronic (NOAEC = 2.8 ppb) toxicity values. The ACR was 8.9. Therefore, the estimated NOAEC in mysid shrimp would be 8.6/8.9 = 0.96

ppb.

* 

### exceeds the LOC for Acute endangered species (RQ > 0.05)

** 

### E xceeds the LOC for Chronic Risk (RQ > 1.0)



## Aquatic Plants

Table 6

- 4 . RQs for Trifloxystrobin for Aquatic Plants from use on alfalfa

Scenario Peak EEC

(ppb) Species

group Non

- endangered

species RQ 1



### (EEC/EC50) Endangered

species. RQ 2



### (EEC/NOEC)

Caalfalfa 0.0102 vascular

non

- vascular 0.005

<0.001 <0.01

n/a

ILalfalfaNMC 0.0108 Vascular

non

- vascular 0.006

<0.001 <0.01

n/a

Mnalfalfa 0.0024 Vascular

non

- vascular 0.001

<0.001 <0.01

n/a

Ncalfalfa 0.0229 Vascular

non

- vascular 0.012

0.001 0.01

n/a

Paalfalfa 0.0117 Vascular

non

- vascular 0.006

<0.001 <0.01

n/a

Txalfalfa 0.0440 Vascular

non

- vascular 0.023

0.001 0.01

n/a

1 

### based on the duckweed EC50 of >1.93 ppm and the green algae EC50 of 0.037 ppm

2 

### based on the duckweed NOAEC of 0.41 ppm



## Birds and Mammals following Chronic Exposure

Table 6

- 5. Chronic RQs for Mammals and Birds Following Seed Treatment on Alflalfa

Crop Risk Quotients a



### Avian (20 g) Mammalian (15 g)

Chronic Chronic

Alfalfa 0.59 0.12

a 

### Chronic RQ = mg kg

- 1 seed / NOAEC



## Terrestrial Plants

Table 6

- 6. RQ values for plants in dry and semi

- aquatic areas exposed to Trifloxistrobin through

runoff and/or spray drift.*

Plant Type Listed Status Dry Semi

- Aquatic

Monocot non

- listed <0.1 <0.1

Monocot listed Cannot be estimated Cannot be estimated

Dicot non

- listed <0.1 <0.1

Dicot listed Cannot be estimated Cannot be estimated

*If RQ > 1.0, the LOC is exceeded, resulting in potential for risk to that plant group.

## 31 

# APPENDIX 7



# EIIS Pesticide Summary Report: General Information

Trifloxystrobin (129112)

Incident # Date County State Certainty Legal. Formul. Appl. Method Total Magnitude



## PLANTS

Bell peppers

I016036

- 004 5/17/2004 Stanislaus CA 1 RU Spray 40 acres



## Grape

I010837

- 005 4/4/2000 YOLO CA 3 RU DF Air blast 44acres

I010837

- 011 5/10/2000 SAN JOAQUIN CA 3 RU DF Air blast 343 acres



## Ornamental

I010837

- 010 4/27/2000 VOLUSIA FL 3 RU DF AIRBLAST; ALL

BROADCAST

I010837

- 080 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837

- 085 10/26/2000 LAKE FL 3 UN DF N/R ALL

I010837

- 083 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837

- 081 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837

- 079 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837

- 078 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837

- 077 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837

- 084 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

Wednesday, December 15, 

### Certainty Code: 0=Unrelated, 1=Unlikely, 2=Possible, 3=Probable, 4=Highly Probable. Page 1 of 2



### Legality Code: RU=Registered Use, M=Misuse, MA=Misuse (Accidental), MI=Misuse (Intentional), U=Unknown.

## 32 Incident # Date County State Certainty Legal. Formul. Appl. Method Total Magnitude



### I010837

- 082 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL



## Tree farm/plantation

I012110

- 001 8/13/2001 DADE FL 2 RU N/R N/R UNKNOWN



## Wheat, spring

I015291

- 045 Cass ND 2 RU EC Broadcast 120 acres



## Wheat, winter

I014029

- 006 4/5/2003 King William VA 2 RU Broadcast 51 acres

Wednesday, December 15, 

### Certainty Code: 0=Unrelated, 1=Unlikely, 2=Possible, 3=Probable, 4=Highly Probable. Page 2 of 2



### Legality Code: RU=Registered Use, M=Misuse, MA=Misuse (Accidental), MI=Misuse (Intentional), U=Unknown.