1

OFFICE OF

PREVENTION, PESTICIDES AND

TOXIC SUBSTANCES

 UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON D.C., 20460

PC Code: 129112

Chemical: Trifloxystrobin

DP Barcode: D378918 

December 17, 2010

MEMORANDUM

SUBJECT: Ecological risk assessment for the new use of Trifloxystrobin [Trifloxystrobin

Flowable Fungicide, EPA Reg. No. 264-989] on alfalfa seed.

TO: Tony Kish, RM 22

Janet Whitehurst, Risk Review Manager

Fungicide Branch/ Registration Division (7505P)

FROM: Pamela Hurley, Ph. D., Senior Toxicologist

Tiffany Mason, Environmental Engineer

Melissa Panger, Ph.D., Senior Biologist

James Hetrick, Ph.D., Senior Science Advisor

Dana Spatz, Branch Chief

Environmental Risk Branch 3

Environmental Fate and Effects Division (7507P)

This memorandum summarizes the ecological risks associated with the proposed new use and 

increased rates of the fungicide trifloxystrobin (PC code: 129112) [Trifloxystrobin Flowable 

Fungicide, 22% a.i. (EPA Reg. No. 264-989)] for alfalfa. The proposed method of application for 

this new product is standard slurry or mist type seed treatment. 

Risk Summary

The proposed new application rate for alfalfa is 0.96 fl oz per 100 lbs of seed (0.0047 lb a.i./acre)

and one application per season.

EFED’s assessment of the new trifloxystrobin use rates indicate that there are no LOC 

exceedances for either freshwater/marine/estuarine aquatic organisms or terrestrial organisms on 

an acute and chronic basis. For terrestrial plants, the EC25 is > 0.25 lb a.i./A. Using that value, 

the LOC for non-listed terrestrial plants would not be exceeded; however, neither a NOAEC nor 
2

an EC05 has been established for terrestrial plants; therefore, risk to endangered terrestrial plant 

species cannot be precluded.

Most required studies for trifloxystrobin have been submitted and found to be acceptable. 

However, an acceptable anaerobic aquatic metabolism study has not been submitted; therefore, 

there is uncertainty associated with the persistence and dissipation of trifloxystrobin in anaerobic 

aquatic environments. This uncertainty is greater for low pH aquatic environments because 

hydrolysis is slower in acidic waters. 

For risk to estuarine/marine invertebrates (mysid) following chronic exposure, the mysid shrimp 

NOAEC of 0.96 ppb was estimated using an acute to chronic ratio derived for daphnids. If the 

acute to chronic ratio is not conserved across species, then risk could be over- or underestimated. Also, trifloxystrobin is expected to degrade relatively rapidly in most environments to 

the major degradate CGA-321113, which is extremely persistent. Therefore, chronic exposure is 

more likely to occur to the degradate CGA-321113 than to trifloxystrobin. The only chronic 

study on the degradate that has been submitted is a reproduction study in daphnids, thus leaving 

an uncertainty in the chronic risk conclusions to other organisms. Nonetheless, trifloxystrobin 

has been shown to be more than 3 orders of magnitude more toxic than the degradate to aquatic 

organisms on an acute basis (see Appendix 1). A similar magnitude difference in toxicity was 

also observed in a daphnid reproduction study. Therefore, risk quotients based on chronic 

toxicity values from trifloxystrobin studies are expected to be protective of organisms exposed to 

the major degradate.

Previous Risk Conclusions

Trifloxystrobin has been previously evaluated by EFED for use on numerous commodities 

including pome fruits, grapes, cucurbits, peanuts, and turf (New Chemical Review, D244219, 

D245281, D254206, D254285, D254286); almonds, fruiting vegetables, hops, potatoes, sugar 

beets, and wheat (D254215, D254220, D254222); root vegetable subgroup 1B (except beet and 

radish) and leaf petiole subgroup 4B (D286427); barley, oats, sweet corn, and soybeans 

(D315580, D314187, D318621, D318623, D363130); grass grown for seed (D332979, 

D332982); asparagus, tropical crops, radish, root vegetables, and strawberries (D331915, 

D339548, D348876). Detailed summaries of these assessments can be found elsewhere

(D309489, D331915). 

Based on previous reviews, EFED concluded that some uses are expected to present risk on an 

acute basis to freshwater and/or estuarine/marine aquatic organisms (fish and invertebrates); 

including Federally-listed endangered and threatened species. Those uses include turf, cucurbits, 

grasses grown for seed, tropical crops, root vegetables, leaf petiole, almonds, fruiting vegetables, 

hops, and potatoes. Ecological risks are not expected for any of the other registered uses based 

on previous assessments.
3

Analysis

Trifloxystrobin is a broad spectrum foliar fungicide that is currently used to control certain 

diseases in crops including, barley, corn, oats, peanuts, pecans, rice, soybeans, wheat and others. 

It also provides both curative and protective properties against a variety of pathogens in a variety 

of crops. Trifloxystrobin is classified as an oximinoacetate fungicide in the strobilurin (sterol 

inhibitors) class. It belongs to the Qol (Group 11) target site of action group and acts by 

interfering with respiration in plant pathogenic fungi and is a potent inhibitor of spore 

germination and mycelial growth. It exhibits no known cross-resistance to other chemical classes 

including anilinopyrimidines (AP). Other products containing the active ingredient 

trifloxystrobin are Flint® (EPA Reg. No 264-777), Stratego (EPA Reg. No 264-779), and 

DistinguishTM (EPA Reg. No. 264-1027) fungicides. 

The proposed label for trifloxystrobin does not give the application rate for the new use on 

alfalfa in lbsa.i./A, but in floz of product per 100 lbs of seed. Therefore, this had to be converted 

to lba.i./A. Likewise, the number of applications is not specified by the label, but one 

application per season was chosen based on crop profiles for alfalfa. The lbs of seeds applied per 

acre and the incorporation depth werebased upon information found in the literature. 

(http://www.uaex.edu/Other_Areas/publications/pdf/FSA-2002.pdf).

Table 1. Crop, application rate, maximum seasonal rate, and reapplication interval for proposed new use 

of trifloxystrobin.

Crop Application Rate Total (season or crop) Reapplication Interval

lba.i./acre No. Applications (days)

Alfalfa 0.0047 1 NA

1

: The label gives the maximum application rate in oz of product/100 lbs seed. This had to be converted to lba.i./A using the following formulas: 

) . . ( 453.4Pr ( ) (% . .) 29.6( ) 1

3

A

lbs a i trifloxystrobin

g

lb

oz

cm a i density AFloz oduct × × × × ≅

The application rate, applications per season, and reapplication intervals are used to estimate 

exposure concentrations (EECs) which are then compared to the toxicity data (Appendices 2 and 

4, respectively).

The EECs are represented by the residues of the fungicide trifloxystrobin. Although its major 

degradation product (CGA-321113) comprises >50% of applied trifloxystrobin in some 

dissipation studies, the available data suggest that CGA-321113 is considerably less toxic to 

aquatic organisms on an acute and chronic basis than trifloxystrobin (see Appendix 1). 

Therefore, it is 'anticipated that risk quotients based on chronic studies using trifloxystrobin 

would be protective of aquatic organisms exposed to CGA-321113 on a chronic basis. The 

available data do not allow for a comparison of the relative toxicities of trifloxystrobin and the 

major degradate in terrestrial organisms. Therefore, based on available data, we assume that the 

degradate CGA-321113 is less toxic to non-target organisms than the parent, and the risk 

assessment will be based on exposure to the parent compound alone.
4

Aquatic Exposures

The EECs in surface water concentrations were estimated with the Tier II PRZM (Version 

3.12.2) and EXAMS (version 2.98.04.06) models linked by the program PE5 (Version 01

compiled 07/23/07). PRZM simulates pesticide fate and transport as a result of leaching, direct 

spray drift, runoff and erosion from an agricultural field.The EXAMS model simulates pesticide 

loading via runoff, erosion, and spray drift into a “standard” farm pond. Detailed description, 

documentation, and direct links for running these models can be found in: 

http://www.epa.gov/oppefed1/models/water/index.htm.

The standard farm pond scenario is used to estimate EECs for ecological exposure. The pond 

scenario, represents a 10-ha corn (all cropped) field that is adjacent to a one hectare by two meter 

deep farm pond, which has neither hydraulic inlets nor outlets (i.e., pesticide cannot leave by 

outflow). Weather and agricultural practices are simulated for 30 years so that the 10-year 

exceedence probability (1-in-10 year) at the site can be estimated. The simulation was generated 

using 30 years of meteorological data, encompassing the years from 1961 to 1990.Guidance for 

using the standard farm pond is located at: http://www.epa.gov/pesticides/trac/science/. 

The surface water trifloxystrobin EECs were estimated for theseed application, using the 

maximum application rates and assuming an annual application for thirty years. Since alfalfa is 

a perennial crop that is reseeded approximately every 3-5 years, the generated EECs should be 

conservative. 

The maximum peak, 21-day, 60-day means for the seed treatment application method is 

summarized in Table 1. It should be noted that while the Texas Alfalfa scenario had the highest 

peak concentration (0.044 μg/L), the highest 21-day (0.007 μg/L) and 60-day (0.003 μg/L) 

means occurred with the North Carolina Alfalfa scenario. 

The chemical properties for trifloxystrobin and input values used for PRZM/EXAMS are

summarized in Appendix 3 and the PRZM/EXAMS output file is in Appendix 5.
5

Table 2. PRZM/EXAMS estimated exposure concentrations (EECs) for the proposed new use.

Crop or 

Crop 

Group

Maximum 

Application 

Rate

Total 

(season 

or crop)

Minimum 

Application 

Interval

Scenario

(State crop)

Scenario

1st 

Application 

date 

(mn-day)

Application 

Method

EECs

lba.i./acre lba.i./acre (days) Peak 21 day 60 day

Alfalfa 0.0047 1 NA

Caalfalfa 16-Jan

Ground

(seed 

treatment)

0.0102 0.0014 0.0005

ILalfalfaNMC 1-Jun 0.0108 0.0018 0.0006

Mnalfalfa 1-Jun 0.0024 0.0006 0.0002

Ncalfalfa 1-Apr 0.0229 0.0070 0.0025

Paalfalfa 16-Apr 0.0117 0.0039 0.0014

Txalfalfa 1-Sep 0.0440 0.0069 0.0024

*Values in bold are the most conservative values.

Terrestrial Exposures

Terrestrial wildlife exposure estimates are typically estimated for birds and mammals following 

the methods of Hoerger and Kenaga (1972) as modified by Fletcher et al. (1994). The conceptual 

framework for this method of estimating terrestrial exposure assumes that organisms are exposed 

to a single pesticide residue via dietary routes in a specific exposure scenario. For seed treatment

applications of trifloxystrobin, terrestrial exposure estimates rely on estimation of a dose (mg a.i. 

bw-1 day-1

) and available pesticide (mg a.i. ft-2). 

Table 3 summarizes the terrestrial EECs for the proposed new use. An application rate of 0.96 

fluid ounces of product per 100 pounds of seed was considered in TREX (v1.4.1); 1 application 

with a maximum seeding rate of 25 pounds/acre.

The EECs on seed were converted to an oral dose and compared with the oral toxicity data. The 

risk assessment for trifloxystrobin uses upper bound (i.e., 90th percentile) predicted residues as 

the measure of exposure. The predicted upper bound residues that may be expected to occur 

immediately following application to alfalfa seed are presented in Table 3.

Table 3. Dose-based Seed Residue Estimates for Proposed Alfalfa Use Rates

Crop Maximum 

Application Rate

lbsa.i./A

Maximum Seed 

Application Rate

mg a.i./kg seed

Avian Nagy 

Dose

mg a.i./kgbw/day

Mammalian 

Nagy Dose

mg a.i./kgbw/day

Available a.i. mg 

a.i. ft-2

Alfalfa <0.01 187.28 47.40 39.68 0.05

TerrPlant (Version 1.1.2) is used to calculate EECs for non-target plant species inhabiting dry 

and semi-aquatic areas. Parameter values for application rate, drift assumption and incorporation 

depth are based upon the use and related application method. For seed treatment, assumption of 

drift alone is not used. A runoff value of 0.01 is utilized based on trifloxystrobin’s solubility, 

which is classified by TerrPlant as <10 mg/L. These EECs are listed in Table 4.
6

Table 4. EECs for Trifloxystrobin. Units in lbs/A.

Description EEC

Runoff to dry areas 0.000047

Runoff to semi-aquatic areas 0.00047

Total for dry areas 0.000094

Total for semi-aquatic areas 0.000517

Effects Data

A tabular summary of the ecotoxicity data for trifloxystrobin is provided in Appendix 1. 

Trifloxystrobin is very highly toxic to aquatic organisms on an acute basis. Fish LC50 values 

range from 14 to 78 ppb, and invertebrate EC50 values range from 8.6 to 29 ppb. Trifloxystrobin 

has been shown to affect survival in fish chronic studies at 7.7 ppb and survival and reproduction 

in invertebrate reproduction studies at 5.9 ppb. No effects were observed in fish and 

invertebrates at 4.3 ppb and 2.8 ppb, respectively. 

Trifloxystrobin is highly toxic to aquatic plants based on the green algae EC50. The green algae 

and duckweed EC50 values were 37 ppb and >1930 ppb, respectively, and the NOAECs were 10 

ppb and 410 ppb, respectively. 

Trifloxystrobin is practically non-toxic to birds (LD50>2000 mg/kg-bw) and mammals 

(LD50>5000 mg/kg-bw) on an acute oral basis. No mortality or signs of toxicity occurred in 

these acute studies. Exposure to trifloxystrobin did not induce reproductive effects at the 

concentrations evaluated in submitted studies in birds (NOAEC = 320 - 500 ppm) or mammals 

(NOAEC = 1500 ppm). 

Trifloxystrobin did not cause >25% inhibition in tier I terrestrial plant toxicity studies at 0.249 

lbsa.i./Acre, which is above the maximum proposed application rate being assessed in this 

evaluation (0.0047 lbsa.i./Acre). Therefore, Tier II studies are not required. However, because 

inhibition was observed, and the data from a Tier I study are not sufficient to allow for 

calculation of a slope, an EC05, or a NOAEC, risk to endangered plant species cannot be assessed 

and, therefore, cannot be precluded. 
7

Risk Estimation 

Risk characterization integrates EECs and toxicity estimates to determine whether the proposed 

uses pose risk to non-target species at levels of concern (LOC) to the Agency. In a deterministic 

approach, a single point estimate of toxicity is divided by an exposure estimate to calculate a risk 

quotient (RQ). The RQ is then compared to Agency LOCs, which serve as criteria for 

categorizing potential risk to non-target organisms (See Appendix 4).

None of the acute or chronic RQs calculated for fish (freshwater and estuarine/marine) or aquatic 

invertebrates (freshwater and estuarine/marine) or RQs for aquatic plants (vascular and nonvascular) exceed 0.01 for any of the scenarios modeled (see Appendices 2 and 6). Therefore, 

none of the aquatic RQs exceeded any of the Agency’s LOCs for aquatic organisms.

Acute terrestrial animal RQs were not calculated for the proposed new uses of trifloxystrobin 

since the available acute oral and dietary did not determine definitive acute toxicity thresholds 

(i.e., bird LD50> 2000 mg/kg; bird LC50> 5050 ppm; mammalian LD50> 5000 mg/kg). There 

were no treatment-related mortalities in any of the avian or mammalian acute toxicity studies. If 

acute RQs were calculated using these data, all avian and mammalian acute RQs would be less 

than LOCs. Additionally, the chronic RQs for birds and mammals are 0.59 and 0.12, 

respectively (see Appendix 6), which are both below the Agency’s chronic risk LOC for these 

taxa.

The acute contact toxicity value for honeybees is >200 ug/bee, which is practically non-toxic. 

Risk estimations for terrestrial invertebrates is not routinely conducted. Based on the acute 

contact toxicity study to honeybees, risk to terrestrial invertebrates is presumed to be negligible.

Using Terrplant and the endpoint of >0.25 lb/A for terrestrial plants, the LOC for non-listed 

terrestrial plants would not be exceeded (See Appendix 6); however, neither a NOAEC nor an 

EC05 has been established for terrestrial plants; therefore, risk to endangered terrestrial plant 

species cannot be precluded.

Risk Description

Non-target Aquatic Animals and Plants

The acute and chronic LOCs are not exceeded for either freshwater fish or invertebrates

(Appendix 6). Minimal risk is presumed for non-endangered and endangered freshwater fish and 

invertebrates from the parent material. Based on the toxicity of CGA-321113 (EC50> 95,000 ppb, 

NOAEC = 3,200 ppb for the most sensitive organism, Daphnia magna), minimal risk to either 

freshwater fish or invertebrates is also expected from the primary degradate (MRIDs 44527503

and 44869701). No additional degradate testing is required at this time.

LOCs were not exceeded for acute or chronic endangered species for estuarine/marine 

invertebrates or fish for the proposed alfalfa use. Likewise, no chronic LOCs were exceeded. 

The mysid shrimp NOAEC of 0.96 ppb was estimated using an acute to chronic ratio derived 
8

using acute and chronic studies in daphnids. If the acute to chronic ratio is not conserved across 

species, then risk could be over- or under-estimated. 

Aquatic Plants

The LOC is not exceeded for either vascular or non-vascular aquatic plants (see Appendix 6). 

Minimal risk is presumed for non-endangered and endangered aquatic plants from the parent 

material. Based on the toxicity of CGA-321113 (EC50 = 77.1 ppm), minimal risk to aquatic 

plants also is expected from the primary degradate (MRID 44527504). No additional degradate 

testing is required at this time.

Trifloxystrobin is expected to degrade relatively rapidly in most environments to the major 

degradate CGA-321113, which is extremely persistent; therefore, chronic exposure is more 

likely to occur with the degradate CGA-321113 than with trifloxystrobin. However, the 

degradate has been shown to be considerably less toxic than trifloxystrobin in aquatic plants, 

fish, and invertebrates on an acute basis, and in aquatic invertebrates on a chronic basis. 

Therefore, exposure to CGA-321113 is not expected to pose either acute or chronic risk to 

aquatic organisms at the Agency’s LOCs.

Non-target Terrestrial Animals and Plants

As stated previously, acute terrestrial animal RQs were not calculated for the proposed new uses 

of trifloxystrobin since the available acute oral and dietary did not determine definitive acute 

toxicity thresholds. When compared to the estimated terrestrial EECs, the acute toxicity 

endpoints were all greater than 1/10 of the EECs; therefore, there is minimal concern for listed 

birds, reptiles, terrestrial-phase amphibians or mammals on an acute exposure basis. Similarly, 

following chronic exposure, the LOCs for both birds and mammals were below the LOC. 

Therefore, there is minimal concern for listed birds, reptiles, terrestrial-phase amphibians and 

mammals following chronic exposure to trifloxistrobin with the currently proposed seed use. 

Again, as stated previously, risk to terrestrial invertebrates is presumed to be negligible.

Risk to non-listed terrestrial plants is not expected for this use; however, due to the fact that 

neither a NOAEC nor an EC05 has been established for terrestrial plants, risk to listed terrestrial 

plant species cannot be precluded. In addition, EFED notes that another strobilurin fungicide has 

been reported to be phytotoxic to fruit and fruit trees at low levels. Therefore, there is a concern 

that trifloxystrobin may also be phytotoxic to fruit trees. EFED's current plant toxicity database 

does not provide information that would allow an assessment of such damage. The registrant 

would need to submit available studies or information on potential phytotoxicity to fruit trees or 

other agronomic crops to allow for an analysis of such risks. Finally, there are a number of 

incident reports indicating damage to terrestrial plants following application of a trifloxistrobin 

formulation (see below and Appendix 7).
9

Incident Reports

Sixteen incident reports are available in the Ecological Incident Information System (EIIS, v. 

2.1), ranging from the year 2000 to 2004. All are incidents with terrestrial plants. One was 

classified as unlikely, 3 were classified as possible and the rest were classified as probable. Six 

were with registered uses and the remainder were undetermined. Two reports were with wheat 

and grape, 10 with ornamentals and the last two were with a tree farm and with bell peppers. 

The majority of the incidents were in Florida. The remaining reports were in California, Virginia 

and North Dakota. See Appendix 7. In the Aggregate Incident Report, there are 4 minor 

incidents with terrestrial plants, ranging from 2006-2008. No incidents were reported in the 

AIMS database (American Bird Conservancy).
10

APPENDIX 1.

Ecotoxicity Profile of Trifloxystrobin

Appendix 1. Table 1. Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk 

Assessment

Comments MRID

Terrestrial Toxicity

Rat acute oral LD50 >5000 mg/kg-bw Acceptable study. 44496622

Rat 2-generation 

reproduction 

NOAEL 1500 mg/kgfeedAcceptable study. No treatment-related 

effects were observed at any concentration 

tested (up to 1500 ppm feed).

44496710

Bird acute oral LD50

(Mallard Duck, Bobwhite 

Quail)

LD50: >2000 - >2250 

mg/kg-bw

Acceptable studies. No mortality or 

treatment-related toxic effects occurred in 

either species.

44496606

44496608 

Bird dietary LC50 (Mallard 

duck and Bobwhite quail)

>5050 mg/kg-feed Acceptable study. No mortality or 

treatment-related toxic effects occurred in 

either species.

44496607 

44496605

Reproductive Toxicity 

NOAEL (Bobwhite)

320 mg/kg-feed Acceptable study. No treatment-related 

effects were observed at any concentration 

tested (up to 320 ppm feed).

44496609

Reproductive Toxicity 

NOAEL (Mallard duck)

500 mg/kg-feed Acceptable study. No treatment-related 

effects were observed at any concentration 

tested (up to 500 ppm feed).

44496610

Honey Bee Contact LD50 >200 ug/bee Acceptable study. No mortality was 

observed.

44496726

Terrestrial Plants (Tier I) None used. EC25 is 

greater than the highest 

concentration tested 

(0.25 lbsa.i./Acre)

Supplemental Study. The study is 

acceptable; however, it does not allow for 

an assessment of risk to endangered species 

because a 13% effect was observed in 

cabbage. The data do not allow for 

derivation of a NOAEC or an EC05.

44496723

Aquatic Toxicity

Rainbow Trout LC50

Degradate CGA-321113

Bluegill Sunfish LC50

14 ppb

>106,000 ppb

54 ppb

Acceptable study

Supplemental study (insufficient number 

of test organisms)

Acceptable study

44502803

44527502

44502802

Daphnia magna EC50 25 ppb Acceptable studies 44496611
11

Appendix 1. Table 1. Toxicity Profile for Trifloxystrobin

Test Type Value Used in Risk 

Assessment

Comments MRID

Degradate CGA-321113 >95,000 ppb Acceptable studies 44527503

Sheepshead minnow LC50 78 ppb Acceptable study 44496612

Oyster shell deposition 

EC50

29 ppb Acceptable study 44496613

Mysid shrimp LC50 8.6 ppb Acceptable study 44496614

Mysid shrimp NOAEC 0.96 ppb No study submitted. The estuarine/marine 

chronic NOAEC was estimated using acute 

to chronic ratios (ACRs) derived from 

daphnid acute (EC50=25 ppb) and chronic 

(NOAEC = 2.8 ppb) toxicity values. The 

ACR was 8.9. Therefore, the estimated 

NOAEC in mysid shrimp would be 8.6/8.9 

= 0.96 ppb.

Not 

applicable

Rainbow Trout Early Life 

Stage

NOAEC: 4.3 ppb Acceptable study. The LOAEC was 7.7 

ppb based on reduced survival. 

44502804

Daphnia magna life cycle

Degradate CGA-321113

NOAEC: 2.8 ppb

NOAEC: 3,200 ppb

Acceptable study. The LOAEC was 5.9 

ppb based on reduced survival and 

reproduction. 

Supplemental study. Water parameter 

measurements and growth endpoints of 

weight and length were not measured

44527503

44869701

Green algae 

Degradate CGA-321113

EC50: 37 ppb

EC50: 78,800 ppb

Acceptable study. The NOAEC was 10 

ppb.

Supplemental study. Test duration was 

only 72 hours; a definitive EC50 was not 

determined

44496724

44527504

Duckweed (Lemnagibba) EC50: >1930 ppb Acceptable study. The NOAEC was 410 

ppb.

44496725
12

APPENDIX2.

Toxicological Endpoints and Summary of Risks

Appendix 2. Table 1. Summary of Risks Identified in this Assessment from the Proposed Trifloxystrobin Uses

Ecosystem at 

Risk

Taxonomic group

(surrogate 

species)

Assessment 

Endpointsa

Toxicity Value Used to 

Evaluate Assessment 

Endpointsb

Conclusions Regarding 

Potential for the Assessment 

Endpoints to be Affected by the 

Proposed New Trifloxystrobin 

Uses

Terrestrial 

ecosystems:

Animals. Treated 

and adjacent fields

Plants

Birds and Reptiles

. Run-off or 

drift onto an 

adjacent field and 

runoff onto a lowlying semi-aquatic 

or wetland area

(Mallard duck

Bobwhite quail)

Survival,

reproduction, 

and 

growth

Survival

LD50: >2000 - >2250 mg/kg-bw

: 

Dietary LC50: >5050 ppm (both 

species)

Growth and Reproduction

NOAEC: 320 ppm to 500 ppm

:

Risk is below the Agency’s LOCs 

for the assessed endpoints. 

Mammals

(Laboratory Rat)

Survival

LD50: >5000 mg/kg-bw

: 

Growth and Reproduction

Risk is below the Agency’s LOCs 

for the assessed endpoints. 

: 

NOAEC: 1500 ppm

Terrestrial plants c Survival and 

growth

Growth and survival

EC25: >0.25 lbsa.i./Acre

: Because neither a NOAEC nor an 

EC05 has been established, risk to 

endangered plant species cannot 

be precluded.

Insects Survival (honey 

bees)

Survival

LD50: >200 ug/bee

: Risk to pollinators appears to be 

lower than levels that are of 

concern to the Agency.

Aquatic 

ecosystems: Tier 

II environment is a 

1 hectare, 2 – m 

deep pond 

 

 

Fresh Water Fish 

and Amphibians

(Rainbow trout)

Survival, 

growth, and 

reproduction of 

individuals and 

communities

Survival: LC50: 14 ppb

Growth and Reproduction

Risk is below the Agency’s LOCs 

for the assessed endpoints.

: 

NOAEC: 4.3 ppb
13

Appendix 2. Table 1. Summary of Risks Identified in this Assessment from the Proposed Trifloxystrobin Uses

Ecosystem at 

Risk

Taxonomic group

(surrogate 

species)

Assessment 

Endpointsa

Toxicity Value Used to 

Evaluate Assessment 

Endpointsb

Conclusions Regarding 

Potential for the Assessment 

Endpoints to be Affected by the 

Proposed New Trifloxystrobin 

Uses

Freshwater 

invertebrates

(Water fleas)

Survival

LC50: 25 ppb

: 

Growth and Reproduction

Risk is below the Agency’s LOCs 

for the assessed endpoints. 

: 

NOAEC: 2.8 ppb

Estuarine/ marine 

fish and 

amphibians

(Sheepshead 

minnow)

Survival Risk is below the Agency’s LOCs 

for the assessed endpoints. 

: LC50: 78 ppb

Estuarine/ marine 

invertebrates

(mysid shrimp)

Survival

LC50: 8.6 ppb

: 

Growth and Reproduction

Risk is below the Agency’s LOCs 

for the assessed endpoints. 

However, the chronic assessment 

was based on an acute to chronic 

ratio (ACR) calculated from 

daphnid studies. If the ACR is 

not conserved across species, 

then risk could be under- or overestimated.

: 

NOAEC: 0.96 ppb (estimated 

using acute to chronic ratio)

Aquatic plants and 

algae

(green algae)

Growth and 

reproduction of 

individual and 

communities of 

aquatic plants 

EC50: 37 ppb; NOAEC: 10 ppb

Growth and Reproduction: Risk is below the Agency’s LOCs 

for the assessed endpoints. 

a

The assessment endpoints measure effects at the individual level; however, they provide insight into potential risks at higher levels 

(i.e., populations). 

b See Tables III-9 and III-10 for additional information on the toxicity studies used to derive these values. c Four species of two families of monocots, of which one is corn; six species of at least four dicot families, of which one is soybeans.
14

APPENDIX3. 

Summary of Environmental Fate Data and Model Input Parameters for Trifloxystrobin.

Environmental Fate Characterization

The major routes of degradation and dissipation for trifloxystrobin are associated with alkalinecatalyzed hydrolysis, microbial-mediated metabolism, photodegradation, and soil binding. 

Hydrolysis of trifloxystrobin is most important under alkaline conditions. Dissipation of 

trifloxystrobin will also occur in the environment by spray drift, in runoff water and eroded soil. 

The major degradate in hydrolysis, metabolism and photodegradation is CGA-321113, the free 

acid degradation product of trifloxystrobin. (D309489). However, CGA-321113 is not 

considered in this assessment due to the fact that the parent is more toxic than the degradate. 

The decline of trifloxystrobin (half-life <3 day) appears to be quite rapid (D254286). The decline 

pattern does not appear to follow a first order kinetics based upon visual inspection, but tended 

to follow a “hockey stick” degradation pattern (a rapid initial decline followed by a slower 

decline), thus thefirst-order half-life was does not accurately describe the decline of 

trifloxystrobin. Due to the rapid decline of trifloxystrobin and disappearance, residues degrade 

more rapidly than estimated by first order kinetics. 

Trifloxystrobin is expected to have low mobility in the environment. The range for the 

Freundlich sorption coefficients (1/N) for trifloxystrobin, show there is no a correlation between 

soil organic matter and Kf. Therefore, the lack of correlation suggests that sorption is controlled 

by factors other than soil organic carbon content. Please see Table 1 below for values of sorption 

coefficients.

Appendix 3. Table 1. Sorption Coefficient Values for Trifloxystrobin 

Parameter Trifloxystorbin

Kf (mL/g) 6.8 to 91.8 (Average 26.9)

1/N 0.88 to 1.30

KOC (mL/goc) 48 to 235

Due to the rapid degradation, low solubility (0.61 mg/L), and relatively low mobility of 

trifloxystrobin, trifloxystrobin is thought to have a limited potential to contaminate water. 

Likewise, due to the low vapor pressure (2.55e-08 mm Hg @ 20 ◦C) and calculated Henry’s Law 

constant (2.2e-08atm m3 mol-1), volatilization from both dry and moist soils and water surfaces is 

not expected. Therefore, volatilization is not an important dissipation pathway.

Chemical names and chemical structure for trifloxystrobin are summarized in Figure 1.
15

Appendix 3. Figure 1. The common name, chemical name, structure for trifloxystrobin, 

and some additional information. 

Common Name

Chemical Name Structure

Trifloxystrobin

(E,E)-α-(methoxyimino)-2-[[[[1-[3-

(trifluoromethyl) phenyl] ethylidene] 

amino] methyl] -methyl ester)

Chemical Abstracts #: 141517-21-7

N

O

H C3

O

CH3

O

O

N

CH3

CF3

Appendix 3. Table 2. Summary environmental fate data and model input parameters for trifloxystrobin 

which were used to estimate the EECs for the aquatic exposure assessment.

Parameter Value Modeling Input Value Source

Molecular Formula C20H19F3N2O4 DP Barcode D254920

Molecular Weight 408.4 408.4 MRID 44052801

Solubility @ 20 ◦C 0.61 mg/L @ pH7 0.61 mg/L MRID 44052801

Vapor Pressure 2.55e-08 mm Hg @ 20 ◦C 2.55e-08 mm Hg @ 20 ◦C DP Barcode D254920

Henry’s Law Constant 2.2e-08atm m3 mol-1 Calculated

Hydrolysis Half-life Stable @ pH 4

55.2 days @ pH7

0.83 days @ pH 9

Stable (0 days)

55.2 days

0.83 days

MRID 44496727

Aquatic Photolysis Halflife14.8 days @pH 5 14.8 days MRID 44496727

Photolysis on soil Halflife2.1 days 2.1 days MRID 44496729

Aerobic Soil 

Metabolism Half-life

(T1⁄2)

0.74, 0.41, and 0.91 days

mean = 0.69 d, std = 0.25 

d

90th percent upper bound 

of mean =

0.96 days

MRID 44496732

MRID 44496730

MRID 44496731

EFED Guidance

Anaerobic Aquatic 

Metabolism Half-life

0.72 days 2.16 days (3 x 0.72) MRID 44496733
16

Appendix 3. Table 2. Summary environmental fate data and model input parameters for trifloxystrobin 

which were used to estimate the EECs for the aquatic exposure assessment.

Parameter Value Modeling Input Value Source

Aerobic Aquatic 

Metabolism Half-life

0.60 days (supplemental)

2 x aerobic soil 

metabolism (0.96 days)

1.92 MRID 44496734

EFED Guidance

Organic Carbon 

Partition Coefficient 

(Koc) (mL/g)

2354, 951, 3064, 3927, 

9871, 951 coefficient of 

determination (r2

) = (Kf vs. 

organic matter = 0.03)

Use mean Kf MRID 44496802

Soil Partition 

Coefficient (Kf )

(mL/g)

(Kf) (texture class) 

 6.8 Loamy sand

 7.7 Sandy loam

16.0 (Low Org Matter)

59.4 (High Org Mattersand)

91.8 (Clay loam) 

33.2 (Silt loam -

6%OM)

Mean = 35.82; Std = 33.8 

mean Kf= 35.821 mL/g

MRID 44496802

Ground Spray Drift

Application Efficiency

0.01

0.99

0.01

0.99

EFED Input Parameter 

Guidance

FEXTRA - foliar apps PRZM Foliar Extraction 0.5 (frac/cm rain) EFED Guidance

PLDKRT - foliar apps PRZM Decay rate of 

Foliage

0 EFED Guidance
17

APPENDIX4.

Risk Characterization

Risk characterization integrates EECs and toxicity estimates to determine whether the proposed 

trifloxystrobin uses pose risk to non-target species at levels of concern to the Agency. In a 

deterministic approach, a single point estimate of toxicity is divided by an exposure estimate to 

calculate a risk quotient (RQ). The RQ is then compared to Agency LOCs, which serve as 

criteria for categorizing potential risk to non-target organisms. LOCs currently address the 

following risk presumption categories:

Risk characterization integrates EECs and toxicity estimates to determine whether the proposed 

trifloxystrobin uses pose risk to non-target species at levels of concern to the Agency. In a 

deterministic approach, a single point estimate of toxicity is divided by an exposure estimate to 

calculate a risk quotient (RQ). The RQ is then compared to Agency LOCs, which serve as 

criteria for categorizing potential risk to non-target organisms. LOCs currently address the 

following risk presumption categories:

Risk presumptions for terrestrial animals

Risk Presumption Risk Quotient (RQ) Level of Concern 

(LOC)

 Birds

Acute Risk EEC1/LC50 0.5

Acute Restricted Use EEC/LC50 or LD50< 50 mg/kg 0.2

Acute Endangered Species EEC/LC50 0.1

Chronic Risk EEC/NOAEC 1

 Wild Mammals

Acute Risk EEC/LC50 0.5

Acute Restricted Use EEC/LC50 or LD50< 50 mg/kg 0.2

Acute Endangered Species EEC/LC50 0.1

Chronic Risk EEC/NOAEC 1

1 abbreviation for Estimated Environmental Concentration (ppm) on avian/mammalian food items 

Risk presumptions for aquatic animals

Risk Presumption RQ LOC

Acute Risk EEC1/LC50 or EC50 0.5

Acute Restricted Use EEC/LC50 or EC50 0.1

Acute Endangered Species EEC/LC50 or EC50 0.05

Chronic Risk EEC/NOAEC 1

1

 EEC = (ppm or ppb) in water
18

Risk presumptions for plants

Risk Presumption RQ LOC

 Plant Inhabiting Terrestrial and Semi-Aquatic Areas 

Acute Risk EEC1/EC25 1

Acute Endangered Species EEC/EC05 or NOAEC 1

Aquatic Plants

Acute Risk EEC2/EC50 1

Acute Endangered Species EEC/EC05 or NOAEC 1

1

 EEC = lbsa.i./A ; 2 EEC = (ppb or ppm) in water 

The risk estimation presents risk quotients calculated for trifloxystrobin and the LOCs (if any) 

that are exceeded. Further discussion of the risk quotients and uncertainties in the assessment is 

presented in the risk description for each taxa assessed. 

The acute and chronic LOCs are not exceeded for either freshwater fish or invertebrates. 

Minimal risk is presumed for non-endangered and endangered freshwater fish or invertebrates 

from the parent material. Based on the toxicity of CGA-321113 (EC50 > 95,000 ppb, NOAEC = 

3,200 ppb for the most sensitive organism, Daphnia magna), minimal risk to either freshwater 

fish or invertebrates is also expected from the primary degradate. No additional degradate testing 

is required at this time.
19

APPENDIX 5.

PRZM/EXAMS output files

stored as Alfalfa-CA.out

Chemical: trifloxystrobin

PRZM environment: CAalfalfa_WirrigOP.txt modified Thuday, 14 June 2007 at 10:20:44

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w93193.dvf modified Wedday, 3 July 2002 at 08:04:24

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.005626 0.00305 0.0008228 0.000288 0.000192 4.735e-005

1962 0.006002 0.003356 0.0008877 0.0003108 0.0002072 5.109e-005

1963 0.006912 0.004312 0.001213 0.0004249 0.0002833 6.985e-005

1964 0.006994 0.004125 0.001117 0.000391 0.0002607 6.41e-005

1965 0.002985 0.002065 0.0006323 0.0002215 0.0001476 3.641e-005

1966 0.01105 0.005966 0.00151 0.0005287 0.0003525 8.691e-005

1967 0.004768 0.002842 0.0008296 0.0002904 0.0001936 4.774e-005

1968 0.00761 0.004114 0.00104 0.0003641 0.0002427 5.968e-005

1969 0.003656 0.002345 0.0006977 0.0002443 0.0001629 4.017e-005

1970 0.004061 0.002411 0.0006695 0.0002344 0.0001563 3.853e-005

1971 0.007107 0.00434 0.001184 0.0004145 0.0002763 6.814e-005

1972 0.009255 0.005054 0.001279 0.0004476 0.0002984 7.337e-005

1973 0.003622 0.002179 0.0005904 0.0002067 0.0001378 3.398e-005

1974 0.008285 0.004439 0.001108 0.0003879 0.0002586 6.376e-005

1975 0.00894 0.00507 0.001314 0.0004601 0.0003068 7.564e-005

1976 0.005953 0.003448 0.001017 0.0003561 0.0002374 5.837e-005

1977 0.005545 0.002919 0.0007594 0.0002658 0.0001772 4.37e-005

1978 0.00852 0.00477 0.00123 0.0004306 0.0002871 7.078e-005

1979 0.00821 0.004401 0.001097 0.0003839 0.000256 6.311e-005

1980 0.004501 0.002778 0.0008372 0.0002931 0.0001954 4.805e-005

1981 0.00475 0.002536 0.0006497 0.0002274 0.0001516 3.738e-005

1982 0.007192 0.004462 0.001239 0.0004339 0.0002893 7.133e-005

1983 0.01138 0.006268 0.001604 0.0005617 0.0003745 9.234e-005

1984 0.004499 0.002629 0.0007318 0.0002562 0.0001708 4.2e-005

1985 0.004824 0.002587 0.0006666 0.0002333 0.0001555 3.835e-005

1986 0.003561 0.00215 0.0005813 0.0002035 0.0001357 3.345e-005

1987 0.006459 0.003487 0.0008738 0.0003059 0.0002039 5.029e-005

1988 0.004334 0.002506 0.0007049 0.0002468 0.0001645 4.045e-005

1989 0.01025 0.005704 0.001461 0.0005116 0.000341 8.409e-005

1990 0.004889 0.002898 0.0007714 0.00027 0.00018 4.439e-005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.01138 0.006268 0.001604 0.0005617 0.0003745 9.234e-005

0.0645161290322581 0.01105 0.005966 0.00151 0.0005287 0.0003525 8.691e-005

0.0967741935483871 0.01025 0.005704 0.001461 0.0005116 0.000341 8.409e-005

0.129032258064516 0.009255 0.00507 0.001314 0.0004601 0.0003068 7.564e-005

0.161290322580645 0.00894 0.005054 0.001279 0.0004476 0.0002984 7.337e-005

0.193548387096774 0.00852 0.00477 0.001239 0.0004339 0.0002893 7.133e-005

0.225806451612903 0.008285 0.004462 0.00123 0.0004306 0.0002871 7.078e-005

0.258064516129032 0.00821 0.004439 0.001213 0.0004249 0.0002833 6.985e-005

0.290322580645161 0.00761 0.004401 0.001184 0.0004145 0.0002763 6.814e-005

0.32258064516129 0.007192 0.00434 0.001117 0.000391 0.0002607 6.41e-005

0.354838709677419 0.007107 0.004312 0.001108 0.0003879 0.0002586 6.376e-005

0.387096774193548 0.006994 0.004125 0.001097 0.0003839 0.000256 6.311e-005

0.419354838709677 0.006912 0.004114 0.00104 0.0003641 0.0002427 5.968e-005

0.451612903225806 0.006459 0.003487 0.001017 0.0003561 0.0002374 5.837e-005

0.483870967741936 0.006002 0.003448 0.0008877 0.0003108 0.0002072 5.109e-005

0.516129032258065 0.005953 0.003356 0.0008738 0.0003059 0.0002039 5.029e-005

0.548387096774194 0.005626 0.00305 0.0008372 0.0002931 0.0001954 4.805e-005

0.580645161290323 0.005545 0.002919 0.0008296 0.0002904 0.0001936 4.774e-005

0.612903225806452 0.004889 0.002898 0.0008228 0.000288 0.000192 4.735e-005

0.645161290322581 0.004824 0.002842 0.0007714 0.00027 0.00018 4.439e-005
20

0.67741935483871 0.004768 0.002778 0.0007594 0.0002658 0.0001772 4.37e-005

0.709677419354839 0.00475 0.002629 0.0007318 0.0002562 0.0001708 4.2e-005

0.741935483870968 0.004501 0.002587 0.0007049 0.0002468 0.0001645 4.045e-005

0.774193548387097 0.004499 0.002536 0.0006977 0.0002443 0.0001629 4.017e-005

0.806451612903226 0.004334 0.002506 0.0006695 0.0002344 0.0001563 3.853e-005

0.838709677419355 0.004061 0.002411 0.0006666 0.0002333 0.0001555 3.835e-005

0.870967741935484 0.003656 0.002345 0.0006497 0.0002274 0.0001516 3.738e-005

0.903225806451613 0.003622 0.002179 0.0006323 0.0002215 0.0001476 3.641e-005

0.935483870967742 0.003561 0.00215 0.0005904 0.0002067 0.0001378 3.398e-005

0.967741935483871 0.002985 0.002065 0.0005813 0.0002035 0.0001357 3.345e-005

0.1 0.0101505 0.0056406 0.0014463 0.00050645 0.00033758 8.3245e-005

Average of yearly averages: 5.58266666666667e-005

Inputs generated by pe5.pl - Novemeber 2006

Data used for this run:

Output File: Alfalfa-CA

Metfile: w93193.dvf

PRZM scenario: CAalfalfa_WirrigOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E-8 atm-m^3/mol

Vapor Pressure vapr 2.55E-8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half-life kdp 2.1 days Half-life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half-life

Hydrolysis: pH 7 55.2 days Half-life

Hydrolysis: pH 9 0.83 days Half-life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 16-6 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17:FILTRA

IPSCND 1

UPTKF

Record 18:PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa-IL.out

Chemical: trifloxystrobin

PRZM environment: ILalfalfaNMC.txt modified Thuday, 14 June 2007 at 10:16:12

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w14842.dvf modified Wedday, 3 July 2002 at 08:04:38

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.00235 0.001408 0.0003923 0.0001376 9.176e-005 2.263e-005

1962 0.00235 0.001367 0.0003725 0.0001306 8.706e-005 2.147e-005

1963 0.003086 0.001736 0.0007032 0.0002467 0.0001645 4.056e-005

1964 0.00235 0.001334 0.0003506 0.0001229 8.191e-005 2.014e-005

1965 0.00235 0.00137 0.0003705 0.0001299 8.659e-005 2.135e-005

1966 0.00235 0.001373 0.0003722 0.0001305 8.701e-005 2.145e-005

1967 0.00235 0.001352 0.0003674 0.0001288 8.589e-005 2.118e-005

1968 0.00235 0.001332 0.0003501 0.0001227 8.179e-005 2.011e-005

1969 0.009279 0.005798 0.0017 0.0005975 0.0003983 9.822e-005
21

1970 0.01092 0.006524 0.001811 0.0006353 0.0004235 0.0001044

1971 0.00235 0.001243 0.000307 0.0001075 7.166e-005 1.767e-005

1972 0.00235 0.001436 0.0004922 0.000173 0.0001154 2.837e-005

1973 0.006542 0.004158 0.001297 0.000455 0.0003033 7.479e-005

1974 0.00353 0.002381 0.0009841 0.0003475 0.0002316 5.712e-005

1975 0.00235 0.001349 0.0004448 0.000156 0.000104 2.565e-005

1976 0.00235 0.001375 0.0003733 0.0001309 8.726e-005 2.146e-005

1977 0.00235 0.001399 0.0003877 0.000136 9.067e-005 2.236e-005

1978 0.00235 0.001359 0.0003644 0.0001277 8.516e-005 2.1e-005

1979 0.00235 0.001374 0.0003765 0.000132 8.802e-005 2.17e-005

1980 0.1706 0.1114 0.03312 0.01162 0.007749 0.001905

1981 0.00235 0.001313 0.0003407 0.0001194 7.957e-005 1.962e-005

1982 0.00235 0.001459 0.0004247 0.0001492 9.947e-005 2.453e-005

1983 0.00235 0.00167 0.0004846 0.0001698 0.0001132 2.792e-005

1984 0.00235 0.001292 0.000347 0.0001216 8.105e-005 1.993e-005

1985 0.00235 0.001516 0.0004725 0.000166 0.0001107 2.728e-005

1986 0.003084 0.001891 0.0007977 0.0002802 0.0001868 4.607e-005

1987 0.05267 0.02904 0.007524 0.002636 0.001757 0.0004333

1988 0.00235 0.001299 0.0003329 0.0001166 7.774e-005 1.912e-005

1989 0.00235 0.00139 0.0003816 0.0001338 8.923e-005 2.2e-005

1990 0.00235 0.001375 0.0003759 0.0001318 8.786e-005 2.167e-005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.1706 0.1114 0.03312 0.01162 0.007749 0.001905

0.0645161290322581 0.05267 0.02904 0.007524 0.002636 0.001757 0.0004333

0.0967741935483871 0.01092 0.006524 0.001811 0.0006353 0.0004235 0.0001044

0.129032258064516 0.009279 0.005798 0.0017 0.0005975 0.0003983 9.822e-005

0.161290322580645 0.006542 0.004158 0.001297 0.000455 0.0003033 7.479e-005

0.193548387096774 0.00353 0.002381 0.0009841 0.0003475 0.0002316 5.712e-005

0.225806451612903 0.003086 0.001891 0.0007977 0.0002802 0.0001868 4.607e-005

0.258064516129032 0.003084 0.001736 0.0007032 0.0002467 0.0001645 4.056e-005

0.290322580645161 0.00235 0.00167 0.0004922 0.000173 0.0001154 2.837e-005

0.32258064516129 0.00235 0.001516 0.0004846 0.0001698 0.0001132 2.792e-005

0.354838709677419 0.00235 0.001459 0.0004725 0.000166 0.0001107 2.728e-005

0.387096774193548 0.00235 0.001436 0.0004448 0.000156 0.000104 2.565e-005

0.419354838709677 0.00235 0.001408 0.0004247 0.0001492 9.947e-005 2.453e-005

0.451612903225806 0.00235 0.001399 0.0003923 0.0001376 9.176e-005 2.263e-005

0.483870967741936 0.00235 0.00139 0.0003877 0.000136 9.067e-005 2.236e-005

0.516129032258065 0.00235 0.001375 0.0003816 0.0001338 8.923e-005 2.2e-005

0.548387096774194 0.00235 0.001375 0.0003765 0.000132 8.802e-005 2.17e-005

0.580645161290323 0.00235 0.001374 0.0003759 0.0001318 8.786e-005 2.167e-005

0.612903225806452 0.00235 0.001373 0.0003733 0.0001309 8.726e-005 2.147e-005

0.645161290322581 0.00235 0.00137 0.0003725 0.0001306 8.706e-005 2.146e-005

0.67741935483871 0.00235 0.001367 0.0003722 0.0001305 8.701e-005 2.145e-005

0.709677419354839 0.00235 0.001359 0.0003705 0.0001299 8.659e-005 2.135e-005

0.741935483870968 0.00235 0.001352 0.0003674 0.0001288 8.589e-005 2.118e-005

0.774193548387097 0.00235 0.001349 0.0003644 0.0001277 8.516e-005 2.1e-005

0.806451612903226 0.00235 0.001334 0.0003506 0.0001229 8.191e-005 2.014e-005

0.838709677419355 0.00235 0.001332 0.0003501 0.0001227 8.179e-005 2.011e-005

0.870967741935484 0.00235 0.001313 0.000347 0.0001216 8.105e-005 1.993e-005

0.903225806451613 0.00235 0.001299 0.0003407 0.0001194 7.957e-005 1.962e-005

0.935483870967742 0.00235 0.001292 0.0003329 0.0001166 7.774e-005 1.912e-005

0.967741935483871 0.00235 0.001243 0.000307 0.0001075 7.166e-005 1.767e-005

0.1 0.0107559 0.0064514 0.0017999 0.00063152 0.00042098 0.000103782

Average of yearly averages: 0.000108269

Inputs generated by pe5.pl - Novemeber 2006

Data used for this run:

Output File: Alfalfa-IL

Metfile: w14842.dvf

PRZM scenario: ILalfalfaNMC.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol
22

Henry's Law Const. henry 2.2E-8 atm-m^3/mol

Vapor Pressure vapr 2.55E-8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half-life kdp 2.1 days Half-life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half-life

Hydrolysis: pH 7 55.2 days Half-life

Hydrolysis: pH 9 0.83 days Half-life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 1-6 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17:FILTRA

IPSCND 1

UPTKF

Record 18:PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa-MN.out

Chemical: trifloxystrobin

PRZM environment: MNalfalfaOP.txt modified Thuday, 14 June 2007 at 10:21:44

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w14914.dvf modified Wedday, 3 July 2002 at 08:05:52

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.00235 0.00141 0.0003937 0.0001381 9.208e-005 2.271e-005

1962 0.00235 0.001473 0.0004527 0.0001593 0.0001062 2.618e-005

1963 0.00235 0.001417 0.0003976 0.0001395 9.303e-005 2.294e-005

1964 0.00235 0.001455 0.0004215 0.0001481 9.872e-005 2.428e-005

1965 0.003579 0.002452 0.0008084 0.000285 0.00019 4.685e-005

1966 0.00235 0.001479 0.0004437 0.000156 0.000104 2.565e-005

1967 0.00235 0.001554 0.0005125 0.0001811 0.0001207 2.976e-005

1968 0.00235 0.001528 0.0004861 0.0001714 0.0001143 2.81e-005

1969 0.00235 0.001647 0.0005764 0.0002052 0.0001368 3.374e-005

1970 0.00235 0.001429 0.0004051 0.0001422 9.481e-005 2.338e-005

1971 0.00235 0.001486 0.000449 0.0001577 0.0001051 2.592e-005

1972 0.00235 0.001431 0.0004063 0.0001426 9.51e-005 2.338e-005

1973 0.00235 0.001499 0.0004516 0.0001589 0.0001059 2.612e-005

1974 0.00235 0.001495 0.0004487 0.0001579 0.0001052 2.595e-005

1975 0.00235 0.001499 0.0004519 0.000159 0.000106 2.614e-005

1976 0.00235 0.001429 0.000405 0.0001422 9.479e-005 2.331e-005

1977 0.00235 0.001475 0.0004354 0.0001531 0.000102 2.516e-005

1978 0.00235 0.001502 0.0004544 0.00016 0.0001066 2.629e-005

1979 0.00235 0.00148 0.0004385 0.0001542 0.0001028 2.534e-005

1980 0.00235 0.00149 0.0004453 0.0001567 0.0001044 2.568e-005

1981 0.00235 0.001549 0.0004899 0.0001729 0.0001152 2.842e-005

1982 0.00235 0.001606 0.0005378 0.0001906 0.0001271 3.134e-005

1983 0.00235 0.001489 0.0004445 0.0001564 0.0001042 2.57e-005

1984 0.00235 0.001489 0.0005778 0.0002041 0.0001361 3.346e-005

1985 0.00235 0.0016 0.000533 0.0001889 0.0001259 3.104e-005

1986 0.00235 0.001446 0.0004166 0.0001463 9.754e-005 2.405e-005

1987 0.00235 0.001395 0.0003843 0.0001348 8.987e-005 2.216e-005

1988 0.00235 0.001287 0.0003272 0.0001146 7.64e-005 1.879e-005

1989 0.00235 0.001507 0.0004578 0.0001611 0.0001074 2.649e-005

1990 0.03843 0.02409 0.007089 0.002492 0.001661 0.0004097

Sorted results
23

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.03843 0.02409 0.007089 0.002492 0.001661 0.0004097

0.0645161290322581 0.003579 0.002452 0.0008084 0.000285 0.00019 4.685e-005

0.0967741935483871 0.00235 0.001647 0.0005778 0.0002052 0.0001368 3.374e-005

0.129032258064516 0.00235 0.001606 0.0005764 0.0002041 0.0001361 3.346e-005

0.161290322580645 0.00235 0.0016 0.0005378 0.0001906 0.0001271 3.134e-005

0.193548387096774 0.00235 0.001554 0.000533 0.0001889 0.0001259 3.104e-005

0.225806451612903 0.00235 0.001549 0.0005125 0.0001811 0.0001207 2.976e-005

0.258064516129032 0.00235 0.001528 0.0004899 0.0001729 0.0001152 2.842e-005

0.290322580645161 0.00235 0.001507 0.0004861 0.0001714 0.0001143 2.81e-005

0.32258064516129 0.00235 0.001502 0.0004578 0.0001611 0.0001074 2.649e-005

0.354838709677419 0.00235 0.001499 0.0004544 0.00016 0.0001066 2.629e-005

0.387096774193548 0.00235 0.001499 0.0004527 0.0001593 0.0001062 2.618e-005

0.419354838709677 0.00235 0.001495 0.0004519 0.000159 0.000106 2.614e-005

0.451612903225806 0.00235 0.00149 0.0004516 0.0001589 0.0001059 2.612e-005

0.483870967741936 0.00235 0.001489 0.000449 0.0001579 0.0001052 2.595e-005

0.516129032258065 0.00235 0.001489 0.0004487 0.0001577 0.0001051 2.592e-005

0.548387096774194 0.00235 0.001486 0.0004453 0.0001567 0.0001044 2.57e-005

0.580645161290323 0.00235 0.00148 0.0004445 0.0001564 0.0001042 2.568e-005

0.612903225806452 0.00235 0.001479 0.0004437 0.000156 0.000104 2.565e-005

0.645161290322581 0.00235 0.001475 0.0004385 0.0001542 0.0001028 2.534e-005

0.67741935483871 0.00235 0.001473 0.0004354 0.0001531 0.000102 2.516e-005

0.709677419354839 0.00235 0.001455 0.0004215 0.0001481 9.872e-005 2.428e-005

0.741935483870968 0.00235 0.001446 0.0004166 0.0001463 9.754e-005 2.405e-005

0.774193548387097 0.00235 0.001431 0.0004063 0.0001426 9.51e-005 2.338e-005

0.806451612903226 0.00235 0.001429 0.0004051 0.0001422 9.481e-005 2.338e-005

0.838709677419355 0.00235 0.001429 0.000405 0.0001422 9.479e-005 2.331e-005

0.870967741935484 0.00235 0.001417 0.0003976 0.0001395 9.303e-005 2.294e-005

0.903225806451613 0.00235 0.00141 0.0003937 0.0001381 9.208e-005 2.271e-005

0.935483870967742 0.00235 0.001395 0.0003843 0.0001348 8.987e-005 2.216e-005

0.967741935483871 0.00235 0.001287 0.0003272 0.0001146 7.64e-005 1.879e-005

0.1 0.00235 0.0016429 0.00057766 0.00020509 0.00013673 3.3712e-005

Average of yearly averages: 3.9601e-005

Inputs generated by pe5.pl - Novemeber 2006

Data used for this run:

Output File: Alfalfa-MN

Metfile: w14914.dvf

PRZM scenario: MNalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E-8 atm-m^3/mol

Vapor Pressure vapr 2.55E-8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half-life kdp 2.1 days Half-life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half-life

Hydrolysis: pH 7 55.2 days Half-life

Hydrolysis: pH 9 0.83 days Half-life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 1-6 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17:FILTRA

IPSCND 1

UPTKF

Record 18:PLVKRT

PLDKRT
24

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa-NC.out

Chemical: trifloxystrobin

PRZM environment: NCalfalfaOP.txt modified Thuday, 14 June 2007 at 10:21:52

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w03812.dvf modified Wedday, 3 July 2002 at 08:05:50

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1965 0.00235 0.001681 0.0006107 0.0002185 0.0001457 3.593e-005

1966 0.00235 0.001769 0.0007132 0.00026 0.0001733 4.274e-005

1967 0.00235 0.001659 0.0005885 0.0002101 0.0001401 3.453e-005

1968 0.003244 0.002362 0.001133 0.0004111 0.000274 6.739e-005

1969 0.00235 0.001686 0.0006165 0.0002209 0.0001472 3.63e-005

1970 0.03884 0.02858 0.01033 0.003693 0.002462 0.0006071

1971 0.00235 0.001698 0.0006293 0.000226 0.0001506 3.714e-005

1972 0.00235 0.001701 0.000633 0.0002275 0.0001516 3.729e-005

1973 0.00235 0.001748 0.0007794 0.0002847 0.0001898 4.68e-005

1974 0.01729 0.01401 0.005535 0.002012 0.001341 0.0003307

1975 0.00235 0.001726 0.0006606 0.0002383 0.0001588 3.917e-005

1976 0.00235 0.001701 0.0006326 0.0002273 0.0001516 3.727e-005

1977 0.01023 0.007189 0.002767 0.0009974 0.0006649 0.000164

1978 0.00235 0.001672 0.0006012 0.000215 0.0001433 3.534e-005

1979 0.01676 0.01273 0.004851 0.001754 0.001169 0.0002883

1980 0.00235 0.001674 0.0006364 0.0002282 0.0001522 3.742e-005

1981 0.00235 0.001618 0.0005485 0.0001948 0.0001299 3.202e-005

1982 0.00235 0.001746 0.000685 0.000248 0.0001653 4.077e-005

1983 0.0361 0.02762 0.01155 0.004247 0.002831 0.0006982

1984 0.004481 0.003378 0.00161 0.0005957 0.0003972 9.766e-005

1985 0.00235 0.00168 0.00079 0.0002843 0.0001896 4.674e-005

1986 0.00235 0.001678 0.0006071 0.0002172 0.0001448 3.571e-005

1987 0.00235 0.001749 0.000777 0.000283 0.0001887 4.652e-005

1988 0.004834 0.003532 0.001513 0.000549 0.000366 9.001e-005

1989 0.00235 0.001792 0.0007431 0.0002689 0.0001793 4.42e-005

1990 0.00235 0.001726 0.0006608 0.0002384 0.0001589 3.919e-005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.037037037037037 0.03884 0.02858 0.01155 0.004247 0.002831 0.0006982

0.0740740740740741 0.0361 0.02762 0.01033 0.003693 0.002462 0.0006071

0.111111111111111 0.01729 0.01401 0.005535 0.002012 0.001341 0.0003307

0.148148148148148 0.01676 0.01273 0.004851 0.001754 0.001169 0.0002883

0.185185185185185 0.01023 0.007189 0.002767 0.0009974 0.0006649 0.000164

0.222222222222222 0.004834 0.003532 0.00161 0.0005957 0.0003972 9.766e-005

0.259259259259259 0.004481 0.003378 0.001513 0.000549 0.000366 9.001e-005

0.296296296296296 0.003244 0.002362 0.001133 0.0004111 0.000274 6.739e-005

0.333333333333333 0.00235 0.001792 0.00079 0.0002847 0.0001898 4.68e-005

0.37037037037037 0.00235 0.001769 0.0007794 0.0002843 0.0001896 4.674e-005

0.407407407407407 0.00235 0.001749 0.000777 0.000283 0.0001887 4.652e-005

0.444444444444444 0.00235 0.001748 0.0007431 0.0002689 0.0001793 4.42e-005

0.481481481481481 0.00235 0.001746 0.0007132 0.00026 0.0001733 4.274e-005

0.518518518518518 0.00235 0.001726 0.000685 0.000248 0.0001653 4.077e-005

0.555555555555556 0.00235 0.001726 0.0006608 0.0002384 0.0001589 3.919e-005

0.592592592592593 0.00235 0.001701 0.0006606 0.0002383 0.0001588 3.917e-005

0.62962962962963 0.00235 0.001701 0.0006364 0.0002282 0.0001522 3.742e-005

0.666666666666667 0.00235 0.001698 0.000633 0.0002275 0.0001516 3.729e-005

0.703703703703704 0.00235 0.001686 0.0006326 0.0002273 0.0001516 3.727e-005

0.740740740740741 0.00235 0.001681 0.0006293 0.000226 0.0001506 3.714e-005

0.777777777777778 0.00235 0.00168 0.0006165 0.0002209 0.0001472 3.63e-005

0.814814814814815 0.00235 0.001678 0.0006107 0.0002185 0.0001457 3.593e-005

0.851851851851852 0.00235 0.001674 0.0006071 0.0002172 0.0001448 3.571e-005

0.888888888888889 0.00235 0.001672 0.0006012 0.000215 0.0001433 3.534e-005

0.925925925925926 0.00235 0.001659 0.0005885 0.0002101 0.0001401 3.453e-005

0.962962962962963 0.00235 0.001618 0.0005485 0.0001948 0.0001299 3.202e-005
25

0.1 0.022933 0.018093 0.0069735 0.0025163 0.0016773 0.00041362

Average of yearly averages: 0.000117247692307692

Inputs generated by pe5.pl - Novemeber 2006

Data used for this run:

Output File: Alfalfa-NC

Metfile: w03812.dvf

PRZM scenario: NCalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E-8 atm-m^3/mol

Vapor Pressure vapr 2.55E-8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half-life kdp 2.1 days Half-life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half-life

Hydrolysis: pH 7 55.2 days Half-life

Hydrolysis: pH 9 0.83 days Half-life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 1-4 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17:FILTRA

IPSCND 1

UPTKF

Record 18:PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa-PA.out

Chemical: trifloxystrobin

PRZM environment: PAalfalfaOP.txt modified Thuday, 14 June 2007 at 10:23:40

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w14751.dvf modified Wedday, 3 July 2002 at 08:06:14

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.01274 0.009912 0.004262 0.001538 0.001026 0.0002529

1962 0.00235 0.001761 0.0006949 0.0002472 0.0001648 4.063e-005

1963 0.00235 0.001714 0.0006437 0.0002293 0.0001528 3.769e-005

1964 0.00235 0.001796 0.0007551 0.0002689 0.0001793 4.408e-005

1965 0.00235 0.001794 0.0007337 0.0002606 0.0001737 4.284e-005

1966 0.00235 0.001805 0.0007534 0.0002698 0.0001799 4.435e-005

1967 0.00235 0.001751 0.0006892 0.0002489 0.0001659 4.092e-005

1968 0.00235 0.001723 0.0006938 0.0002497 0.0001665 4.094e-005

1969 0.00235 0.001721 0.0006498 0.0002308 0.0001539 3.794e-005

1970 0.00235 0.001773 0.0007439 0.0002646 0.0001764 4.35e-005

1971 0.00235 0.001783 0.000726 0.0002603 0.0001735 4.279e-005

1972 0.0143 0.01097 0.004558 0.00163 0.001087 0.0002673

1973 0.00235 0.001779 0.0007277 0.0002627 0.0001751 4.318e-005

1974 0.00235 0.001678 0.0006046 0.0002145 0.000143 3.526e-005

1975 0.00235 0.001834 0.0008092 0.0002895 0.000193 4.759e-005

1976 0.00235 0.00171 0.00064 0.0002286 0.0001524 3.747e-005

1977 0.00235 0.001726 0.0006725 0.000239 0.0001593 3.928e-005

1978 0.00235 0.001876 0.0008403 0.0003008 0.0002006 4.945e-005
26

1979 0.00235 0.001774 0.0007128 0.0002545 0.0001697 4.184e-005

1980 0.00235 0.001724 0.000654 0.0002322 0.0001548 3.807e-005

1981 0.00235 0.001701 0.0006289 0.0002237 0.0001492 3.678e-005

1982 0.00235 0.00183 0.0007866 0.000282 0.000188 4.636e-005

1983 0.00235 0.001806 0.0008124 0.0002943 0.0001962 4.837e-005

1984 0.00235 0.001826 0.0007829 0.0002824 0.0001882 4.629e-005

1985 0.00235 0.001736 0.0006681 0.0002384 0.0001589 3.919e-005

1986 0.1051 0.08226 0.03301 0.01176 0.00784 0.001933

1987 0.00235 0.001767 0.0007031 0.0002506 0.0001671 4.119e-005

1988 0.00235 0.001791 0.0007337 0.0002623 0.0001748 4.299e-005

1989 0.00235 0.001778 0.0007193 0.000258 0.000172 4.241e-005

1990 0.00235 0.001737 0.0006709 0.0002401 0.0001601 3.947e-005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.1051 0.08226 0.03301 0.01176 0.00784 0.001933

0.0645161290322581 0.0143 0.01097 0.004558 0.00163 0.001087 0.0002673

0.0967741935483871 0.01274 0.009912 0.004262 0.001538 0.001026 0.0002529

0.129032258064516 0.00235 0.001876 0.0008403 0.0003008 0.0002006 4.945e-005

0.161290322580645 0.00235 0.001834 0.0008124 0.0002943 0.0001962 4.837e-005

0.193548387096774 0.00235 0.00183 0.0008092 0.0002895 0.000193 4.759e-005

0.225806451612903 0.00235 0.001826 0.0007866 0.0002824 0.0001882 4.636e-005

0.258064516129032 0.00235 0.001806 0.0007829 0.000282 0.000188 4.629e-005

0.290322580645161 0.00235 0.001805 0.0007551 0.0002698 0.0001799 4.435e-005

0.32258064516129 0.00235 0.001796 0.0007534 0.0002689 0.0001793 4.408e-005

0.354838709677419 0.00235 0.001794 0.0007439 0.0002646 0.0001764 4.35e-005

0.387096774193548 0.00235 0.001791 0.0007337 0.0002627 0.0001751 4.318e-005

0.419354838709677 0.00235 0.001783 0.0007337 0.0002623 0.0001748 4.299e-005

0.451612903225806 0.00235 0.001779 0.0007277 0.0002606 0.0001737 4.284e-005

0.483870967741936 0.00235 0.001778 0.000726 0.0002603 0.0001735 4.279e-005

0.516129032258065 0.00235 0.001774 0.0007193 0.000258 0.000172 4.241e-005

0.548387096774194 0.00235 0.001773 0.0007128 0.0002545 0.0001697 4.184e-005

0.580645161290323 0.00235 0.001767 0.0007031 0.0002506 0.0001671 4.119e-005

0.612903225806452 0.00235 0.001761 0.0006949 0.0002497 0.0001665 4.094e-005

0.645161290322581 0.00235 0.001751 0.0006938 0.0002489 0.0001659 4.092e-005

0.67741935483871 0.00235 0.001737 0.0006892 0.0002472 0.0001648 4.063e-005

0.709677419354839 0.00235 0.001736 0.0006725 0.0002401 0.0001601 3.947e-005

0.741935483870968 0.00235 0.001726 0.0006709 0.000239 0.0001593 3.928e-005

0.774193548387097 0.00235 0.001724 0.0006681 0.0002384 0.0001589 3.919e-005

0.806451612903226 0.00235 0.001723 0.000654 0.0002322 0.0001548 3.807e-005

0.838709677419355 0.00235 0.001721 0.0006498 0.0002308 0.0001539 3.794e-005

0.870967741935484 0.00235 0.001714 0.0006437 0.0002293 0.0001528 3.769e-005

0.903225806451613 0.00235 0.00171 0.00064 0.0002286 0.0001524 3.747e-005

0.935483870967742 0.00235 0.001701 0.0006289 0.0002237 0.0001492 3.678e-005

0.967741935483871 0.00235 0.001678 0.0006046 0.0002145 0.000143 3.526e-005

0.1 0.011701 0.0091084 0.00391983 0.00141428 0.00094346 0.000232555

Average of yearly averages: 0.000119469

Inputs generated by pe5.pl - Novemeber 2006

Data used for this run:

Output File: Alfalfa-PA

Metfile: w14751.dvf

PRZM scenario: PAalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E-8 atm-m^3/mol

Vapor Pressure vapr 2.55E-8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half-life kdp 2.1 days Half-life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife
27

Hydrolysis: pH 5 500 days Half-life

Hydrolysis: pH 7 55.2 days Half-life

Hydrolysis: pH 9 0.83 days Half-life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 16-4 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17:FILTRA

IPSCND 1

UPTKF

Record 18:PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)

stored as Alfalfa-TX.out

Chemical: trifloxystrobin

PRZM environment: TXalfalfaOP.txt modified Thuday, 14 June 2007 at 10:23:54

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 05:14:07

Metfile: w13958.dvf modified Wedday, 3 July 2002 at 08:06:24

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly

1961 0.00235 0.001216 0.0003101 0.0001086 7.243e-005 1.786e-005

1962 0.00235 0.001184 0.0003455 0.000121 8.066e-005 1.989e-005

1963 0.00235 0.001137 0.0002637 9.231e-005 6.154e-005 1.517e-005

1964 0.00235 0.00119 0.000289 0.0001012 6.745e-005 1.659e-005

1965 0.00235 0.001168 0.0002754 9.641e-005 6.427e-005 1.585e-005

1966 0.00235 0.00123 0.0003558 0.0001247 8.312e-005 2.05e-005

1967 0.04422 0.02639 0.007109 0.002491 0.001661 0.0004095

1968 0.01428 0.007978 0.002227 0.0007807 0.0005205 0.000128

1969 0.00235 0.001558 0.0004113 0.000144 9.6e-005 2.367e-005

1970 0.07344 0.03791 0.009187 0.003216 0.002144 0.0005287

1971 0.00235 0.001202 0.0002925 0.0001024 6.827e-005 1.683e-005

1972 0.00235 0.001129 0.0002607 9.125e-005 6.083e-005 1.496e-005

1973 0.00235 0.001195 0.0002979 0.0001043 6.953e-005 1.714e-005

1974 0.00235 0.001364 0.0003672 0.0001287 8.582e-005 2.116e-005

1975 0.00235 0.001292 0.0003297 0.0001155 7.699e-005 1.898e-005

1976 0.07821 0.04101 0.01015 0.003555 0.00237 0.0005828

1977 0.00235 0.001102 0.0002524 8.837e-005 5.891e-005 1.453e-005

1978 0.0291 0.01505 0.003662 0.001282 0.0008548 0.0002108

1979 0.00235 0.001245 0.0003077 0.0001078 7.184e-005 1.771e-005

1980 0.00235 0.001137 0.0004375 0.0001532 0.0001021 2.512e-005

1981 0.04155 0.02092 0.005132 0.001797 0.001198 0.0002954

1982 0.003986 0.002147 0.0005938 0.0002079 0.0001386 3.417e-005

1983 0.00235 0.001229 0.0003009 0.0001054 7.023e-005 1.732e-005

1984 0.006377 0.003469 0.001039 0.0003641 0.0002427 5.968e-005

1985 0.00235 0.001194 0.0003121 0.0001093 7.286e-005 1.796e-005

1986 0.0315 0.01542 0.003895 0.001364 0.0009091 0.0002242

1987 0.00235 0.001199 0.0003288 0.0001152 7.682e-005 1.894e-005

1988 0.00235 0.001134 0.0002625 9.189e-005 6.126e-005 1.506e-005

1989 0.00235 0.001197 0.0002874 0.0001006 6.707e-005 1.654e-005

1990 0.00235 0.001164 0.0002757 9.651e-005 6.434e-005 1.587e-005

Sorted results

Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly

0.032258064516129 0.07821 0.04101 0.01015 0.003555 0.00237 0.0005828

0.0645161290322581 0.07344 0.03791 0.009187 0.003216 0.002144 0.0005287

0.0967741935483871 0.04422 0.02639 0.007109 0.002491 0.001661 0.0004095

0.129032258064516 0.04155 0.02092 0.005132 0.001797 0.001198 0.0002954

0.161290322580645 0.0315 0.01542 0.003895 0.001364 0.0009091 0.0002242

0.193548387096774 0.0291 0.01505 0.003662 0.001282 0.0008548 0.0002108

0.225806451612903 0.01428 0.007978 0.002227 0.0007807 0.0005205 0.000128

0.258064516129032 0.006377 0.003469 0.001039 0.0003641 0.0002427 5.968e-005
28

0.290322580645161 0.003986 0.002147 0.0005938 0.0002079 0.0001386 3.417e-005

0.32258064516129 0.00235 0.001558 0.0004375 0.0001532 0.0001021 2.512e-005

0.354838709677419 0.00235 0.001364 0.0004113 0.000144 9.6e-005 2.367e-005

0.387096774193548 0.00235 0.001292 0.0003672 0.0001287 8.582e-005 2.116e-005

0.419354838709677 0.00235 0.001245 0.0003558 0.0001247 8.312e-005 2.05e-005

0.451612903225806 0.00235 0.00123 0.0003455 0.000121 8.066e-005 1.989e-005

0.483870967741936 0.00235 0.001229 0.0003297 0.0001155 7.699e-005 1.898e-005

0.516129032258065 0.00235 0.001216 0.0003288 0.0001152 7.682e-005 1.894e-005

0.548387096774194 0.00235 0.001202 0.0003121 0.0001093 7.286e-005 1.796e-005

0.580645161290323 0.00235 0.001199 0.0003101 0.0001086 7.243e-005 1.786e-005

0.612903225806452 0.00235 0.001197 0.0003077 0.0001078 7.184e-005 1.771e-005

0.645161290322581 0.00235 0.001195 0.0003009 0.0001054 7.023e-005 1.732e-005

0.67741935483871 0.00235 0.001194 0.0002979 0.0001043 6.953e-005 1.714e-005

0.709677419354839 0.00235 0.00119 0.0002925 0.0001024 6.827e-005 1.683e-005

0.741935483870968 0.00235 0.001184 0.000289 0.0001012 6.745e-005 1.659e-005

0.774193548387097 0.00235 0.001168 0.0002874 0.0001006 6.707e-005 1.654e-005

0.806451612903226 0.00235 0.001164 0.0002757 9.651e-005 6.434e-005 1.587e-005

0.838709677419355 0.00235 0.001137 0.0002754 9.641e-005 6.427e-005 1.585e-005

0.870967741935484 0.00235 0.001137 0.0002637 9.231e-005 6.154e-005 1.517e-005

0.903225806451613 0.00235 0.001134 0.0002625 9.189e-005 6.126e-005 1.506e-005

0.935483870967742 0.00235 0.001129 0.0002607 9.125e-005 6.083e-005 1.496e-005

0.967741935483871 0.00235 0.001102 0.0002524 8.837e-005 5.891e-005 1.453e-005

0.1 0.043953 0.025843 0.0069113 0.0024216 0.0016147 0.00039809

Average of yearly averages: 9.503e-005

Inputs generated by pe5.pl - Novemeber 2006

Data used for this run:

Output File: Alfalfa-TX

Metfile: w13958.dvf

PRZM scenario: TXalfalfaOP.txt

EXAMS environment file: pond298.exv

Chemical Name: trifloxystrobin

Description Variable Name Value Units Comments

Molecular weight mwt 408.4 g/mol

Henry's Law Const. henry 2.2E-8 atm-m^3/mol

Vapor Pressure vapr 2.55E-8 torr

Solubility sol 0.61 mg/L

Kd Kd mg/L

Koc Koc 35.82 mg/L

Photolysis half-life kdp 2.1 days Half-life

Aerobic Aquatic Metabolism kbacw 1.92 days Halfife

Anaerobic Aquatic Metabolism kbacs 2.16 days Halfife

Aerobic Soil Metabolism asm 0.96 days Halfife

Hydrolysis: pH 5 500 days Half-life

Hydrolysis: pH 7 55.2 days Half-life

Hydrolysis: pH 9 0.83 days Half-life

Method: CAM 5 integer See PRZM manual

Incorporation Depth: DEPI 0.635 cm

Application Rate: TAPP 0.0047 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 1-9 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17:FILTRA

IPSCND 1

UPTKF

Record 18:PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none none, monthly or total(average of entire run)
29

APPENDIX 6. 

Risk Quotient Tables for Aquatic and Terrestrial Animals and Plants 

Freshwater Fish and Invertebrates, acute risk 

Table 6-1. Acute RQs for Trifloxystrobin for Freshwater Fish and 

Invertebrates from use on alfalfa

Scenario Peak EEC 

( μg/L)

Fish RQ1

(EEC/LC50)

Invertebrate RQ2

(EEC/EC50)

Caalfalfa 0.0102 0.001 <0.001

ILalfalfaNMC 0.0108 0.001 <0.001

Mnalfalfa 0.0024 <0.001 <0.001

Ncalfalfa 0.0229 0.002 0.001

Paalfalfa 0.0117 0.001 <0.001

Txalfalfa 0.0440 0.003 0.002 1 based on the rainbow trout EC50 of 14 ppb 2 based on the water flea EC50 of 25 ppb *

Exceeds the LOC for Acute endangered species (RQ ≥ 0.05)

Freshwater Fish and Invertebrates, chronic risk

Table 6-2. Chronic RQs for Trifloxystrobin for Freshwater Fish and Invertebrates from use on alfalfa

Scenario 60-day Avg

EEC

( μg/L)

21-day

Avg EEC

( μg/L)

Fish RQ1

(EEC/NOEC)

Invertebrate RQ2 (EEC/NOEC)

Caalfalfa 0.0005 0.0014 <0.001 0.001

ILalfalfaNMC 0.0006 0.0018 <0.001 0.001

Mnalfalfa 0.0002 0.0006 <0.001 <0.001

Ncalfalfa 0.0025 0.0070 0.001 0.002

Paalfalfa 0.0014 0.0039 <0.001 0.001

Txalfalfa 0.0024 0.0069 0.001 0.002 1 based on the rainbow trout early life-stage NOAEC of 4.3 ppb 2 based on the water flea life stage NOAEC of 2.8 ppb

Marine/Estuarine Fish and Invertebrates Acute and Chronic Risk

Table 6-3. Acute and Chronic RQsfor Trifloxystrobin for Estuarine/Marine Organisms from useon 

alfalfa

EEC

Modeled

Peak 

EEC 

(μg/L)

60-day 

Avg

EEC

ppb

21-day

Avg EEC

ppb

Acute Fish 

RQ1

(EEC/LC50)

Acute 

Invertebrate 

RQ2

(EEC/LC50)

Chronic Fish 

RQ 

(EEC/NOEC)

Chronic 

Invertebrate 

RQ3

(EEC/NOEC)

Caalfalfa 0.0102 <0.001 0.001 No Data 0.002

ILalfalfaNMC 0.0108 0.0005 0.0014 <0.001 0.001 No Data 0.002

Mnalfalfa 0.0024 0.0006 0.0018 <0.001 <0.001 No Data 0.001

Ncalfalfa 0.0229 0.0002 0.0006 <0.001 0.003 No Data 0.007

Paalfalfa 0.0117 0.0025 0.0070 <0.001 0.001 No Data 0.004

Txalfalfa 0.0440 0.0014 0.0039 0.001 0.005 No Data 0.007 1 based on the sheepshead minnow LC50 of 78 ppb

2 based on the mysid shrimp LC50 of 8.6 ppb
30

3

The estuarine/marine chronic NOAEC was estimated using acute to chronic ratios (ACRs) derived from daphnid acute (EC50=25 ppb) and 

chronic (NOAEC = 2.8 ppb) toxicity values. The ACR was 8.9. Therefore, the estimated NOAEC in mysid shrimp would be 8.6/8.9 = 0.96 

ppb.

* exceeds the LOC for Acute endangered species (RQ >0.05) ** Exceeds the LOC for Chronic Risk (RQ > 1.0)

Aquatic Plants

Table 6-4. RQs for Trifloxystrobin for Aquatic Plants from use on alfalfa

Scenario Peak EEC

(ppb)

Species

group

Non-endangered

species RQ1

(EEC/EC50)

Endangered 

species. RQ2

(EEC/NOEC)

Caalfalfa 0.0102

vascular

non-vascular

0.005

<0.001 

<0.01

n/a

ILalfalfaNMC 0.0108

Vascular

non-vascular

0.006

<0.001

<0.01

n/a

Mnalfalfa 0.0024

Vascular

non-vascular

0.001

<0.001

<0.01

n/a

Ncalfalfa 0.0229

Vascular

non-vascular

0.012

0.001

0.01

n/a

Paalfalfa 0.0117

Vascular

non-vascular

0.006

<0.001 

<0.01

n/a

Txalfalfa 0.0440

Vascular

non-vascular

0.023

0.001

0.01

n/a

1 based on the duckweed EC50 of >1.93 ppm and the green algae EC50 of 0.037 ppm

2 based on the duckweed NOAEC of 0.41 ppm

Birds and Mammals following Chronic Exposure

Table 6-5. Chronic RQs for Mammals and Birds Following Seed Treatment on Alflalfa

Crop

Risk Quotientsa

Avian (20 g) Mammalian (15 g)

Chronic Chronic

Alfalfa 0.59 0.12

a

Chronic RQ = mg kg-1 seed / NOAEC

Terrestrial Plants

Table 6-6. RQ values for plants in dry and semi-aquatic areas exposed to Trifloxistrobin through 

runoff and/or spray drift.*

Plant Type Listed Status Dry Semi-Aquatic

Monocot non-listed <0.1 <0.1

Monocot listed Cannot be estimated Cannot be estimated

Dicot non-listed <0.1 <0.1

Dicot listed Cannot be estimated Cannot be estimated

*If RQ > 1.0, the LOC is exceeded, resulting in potential for risk to that plant group.
31

APPENDIX 7

EIIS Pesticide Summary Report: General Information

Trifloxystrobin (129112)

Incident # Date County State Certainty Legal. Formul. Appl. Method Total Magnitude

PLANTS

Bell peppers

I016036-004 5/17/2004 Stanislaus CA 1 RU Spray 40 acres

Grape

I010837-005 4/4/2000 YOLO CA 3 RU DF Air blast 44acres

I010837-011 5/10/2000 SAN JOAQUIN CA 3 RU DF Air blast 343 acres

Ornamental

I010837-010 4/27/2000 VOLUSIA FL 3 RU DF AIRBLAST; ALL

BROADCAST

I010837-080 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837-085 10/26/2000 LAKE FL 3 UN DF N/R ALL

I010837-083 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837-081 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837-079 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837-078 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837-077 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

I010837-084 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

Wednesday, December 15, Certainty Code: 0=Unrelated, 1=Unlikely, 2=Possible, 3=Probable, 4=Highly Probable. Page 1 of 2

Legality Code: RU=Registered Use, M=Misuse, MA=Misuse (Accidental), MI=Misuse (Intentional), U=Unknown.
32

Incident # Date County State Certainty Legal. Formul. Appl. Method Total Magnitude

I010837-082 10/26/2000 VOLUSIA FL 3 UN DF N/R ALL

Tree farm/plantation

I012110-001 8/13/2001 DADE FL 2 RU N/R N/R UNKNOWN

Wheat, spring

I015291-045 Cass ND 2 RU EC Broadcast 120 acres

Wheat, winter

I014029-006 4/5/2003 King William VA 2 RU Broadcast 51 acres

Wednesday, December 15, Certainty Code: 0=Unrelated, 1=Unlikely, 2=Possible, 3=Probable, 4=Highly Probable. Page 2 of 2

Legality Code: RU=Registered Use, M=Misuse, MA=Misuse (Accidental), MI=Misuse (Intentional), U=Unknown.