Effluent Quality Assessment 

Kinder Morgan Ammonia Facility 

Hillsborough County 

NPDES #FL0000264 

Sampled July 28, 2008 

January 2009

Biology Section 

Bureau of Laboratories 

Division of Environmental Assessment and Restoration 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1

Kinder Morgan Ammonia Facility (formerly IMC Port Sutton Ammonia Terminal), 4801 Port Sutton 

Rd., Tampa, Hillsborough County, Florida, 33619. NPDES #FL0000264. Effluent samples for this 

facility were collected on July 28, 2008. 

Introduction 

Kinder Morgan Ammonia Facility is a bulk anhydrous ammonia storage facility. The terminal 

receives, stores, and distributes anhydrous ammonia used in the offsite manufacture of chemical 

fertilizers (principally ammonium phosphate products) for agricultural use. The facility receives 

anhydrous ammonia by ship, stores the refrigerated liquid product in a 50,000 ton tank, and 

distributes the product by pipeline, truck, and rail. 

The facility utilizes a new water intake system, which uses the Tampa Electric Company’s 

(TECO) effluent discharge from the Port Sutton Channel, to provide once-through non-contact 

heating water for the ammonia. The once-through, non-contact heating water and stormwater 

runoff are continuously discharged through Outfall D-006, a concrete stilling-basin flowing over a 

rectangular weir, to Port Sutton Channel, a Class III marine water body. The D-006 outfall line is 

approximately 0.76 m (2.5 feet) in length, and the point of discharge is located approximately at 

latitude 27°54’14” N, longitude 82°24’34” W. The facility does not have a permitted discharge 

limit. The average annual daily discharge from July 2007 through June 2008 was 5.15 million 

gallons per day. 

On rare, emergency-only conditions, groundwater will be pumped from the two deep wells for use 

as once-through, non-contact heating water, which shall then be discharged through Outfall D006. 

The facility has no Administrative Orders, Consent Orders or mixing zones. A review of Discharge 

Monitoring Report (DMR) data for the past year, revealed only one permit violation (Feb. 2007 – 

Radium 226+228 value 7.90 pCi/L; permitted value 5.0 pCi/L). Facility inspections have 

questioned the calibration of the flow meter, and a warning letter has been issued for failure to 

perform acute toxicity tests when using groundwater pumping and the emergency outfall. DMR 

trends over the past 5 years have proven to be stable. 

Previous FYI results are not relevant because the heating water source was changed from 

groundwater pumping to neighboring power plant discharge. The change occurred in May 2005. 

(Facility information provided by Jacquelyn Champion of FDEP BMMR Phosphate Management, 

Southwest District). 

Methods 

Samples were collected from the EFF-06 NPDES sampling point for Outfall D-006 by Jacquelyn 

Champion, Scott Rose, and Mike Lynch (FDEP BMMR Phosphate Management SW District) 

following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOPs are available on the 

web at: http://www.floridadep.org/labs/qa/sops.htm. 

See Appendix A for the completed chain of custody form. 

The 48-hour acute screening toxicity tests and algal growth potential test were performed 

following internal DEP SOPs TA07_03, TA07_04, and TA08_01 through TA08_09. All internal 

DEP SOPs are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. 

Toxicity Test Results 

Tests were performed July 29 - 31, 2008. 
2

Americamysis bahia 48-hour acute screening bioassay – LC50 > 100%, 5%* mortality in 100% 

 sample at 48 hours 

Menidia beryllina 48-hour acute screening bioassay – LC50 > 100%, 10%* mortality in 100% 

 sample at 48 hours 

The bioassay sample was not acutely toxic to the test organisms. 

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 standard 

reference toxicant (SRT) tests and the bench sheets and statistical analyses of the SRT tests that 

correspond to these facility tests. See Appendix D for test organism receipts. 

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides 

confidence that the effluent meets the acute toxicity criterion of Florida’s Surface Water Quality 

Standards (Rule 62-302.500 (1)(a)4, F.A.C.). Mortality between 20-50% indicates low to 

moderate levels of toxicity, and further action may be required. Mortality of >50% indicates that 

the effluent fails to meet the minimum requirement to discharge to waters of the state (Rule 62-

4.244(3)(a), F.A.C.). 

Algal Growth Potential 

The effluent Algal Growth Potential (AGP) was 3.0 mg dry wt/L U (U - Material analyzed for but 

not detected; value reported is the method detection limit) of the saltwater species, Dunaliella 

tertiolecta. Raschke and Shultz found that AGP values above 10.0 mg dry weight/L represent a 

“problem” threshold for marine receiving waters, implying nutrient enrichment (personal 

communication, Marshall Faircloth, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). The 

analytical chemistry suggests that the effluent is nitrogen-limited (Table 1; Maloney et al. 1974). 

See Table 1 for AGP results. 

Chemistry Results 

Total residual chlorine and total ammonia were not detected in the bioassay sample in the 

laboratory. The total ammonia concentration in the sample collected and preserved for chemical 

analysis was 0.023 mg/L. Based on the pH, salinity, and temperature of the effluent as collected, 

the calculated unionized ammonia concentration was ≤0.02 mg/L. 

Fluoride, total alpha particles and combined radium 226+228 were detected in the effluent at 

levels that comply with Class III marine quality criteria (62-302.530, F.A.C.) and/or permit limits. 

Arsenic and oils and greases were detected between the laboratory method detection limits 

(MDL) and practical quantitation limits (PQL). 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See 

Appendix E for a complete list of chemical analyses performed. See Appendix F for bioassay lab 

equipment. 

Conclusion 

The effluent sample collected from Outfall D-006 for this facility on July 28, 2008, was not acutely 

toxic to either test species during the 48-hour acute screening bioassays. The effluent AGP result 

was less than the "problem” threshold for marine receiving waters. Effluent water quality samples 

did not exceed any applicable water quality standards or violate any permit conditions. 
3

Literature Cited 

Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: 

Bottle Test. National Environmental Research Center Office of Research and 

Development. U. S. EPA, Corvallis, Oregon. 43 p. 

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data 

assessment. J. Wat. Poll. Cont. Fed. 59(4): 222-227. 
4

Inorganic Total

Location N:P ratio N:P ratio

Effluent 3.0 A U 0.1 2.4

A - Value reported is the mean of two or more determinations

U - Material analyzed for but not detected; value reported is the method detection limit

Kinder Morgan Ammonia Facility NPDES# FL0000264

Table 1. Measured algal growth potential (AGP; mg dry weight/L) of the 

marine species Dunaliella tertiolecta and ratios of nitrogen to 

phosphorus for samples collected from Kinder Morgan Ammonia Facility 

on July 28, 2008.

AGP

(measured)
5

Kinder Morgan Ammonia Facility 

NPDES #FL0000264

Class III 

Criteria

Effluent 

Limits

Aluminum ≤ 1,500 - 180 U

Arsenic ≤ 50 - 3 I

Cadmium ≤ 8.8 - 0.06 U

Copper ≤ 3.7 - 1.5 UJ

Iron ≤ 300 - 90 U

Lead ≤ 8.5 - 0.6 U

Nickel ≤ 8.3 - 0.75 U

Selenium ≤ 71 - 1.5 U

Silver ≤ 2.3 - 0.075 U

Zinc ≤ 86 - 15 U

Ortho-phosphate - - 0.22 A

Total Phosphorus - Report 0.32

Total Ammonia - Report 0.023

Un-ionized Ammonia - Report ≤ 0.02 c

Nitrate and Nitrite - - 0.004 U

Total Kjeldahl Nitrogen - - 0.75 J

Organic Nitrogen - - 0.727 c

Total Nitrogen - Report 0.752 c

Dissolved Oxygen (mg/L) ≥ 4.0 ≥ 4.0 d 7.0

pH (SU) 6.5 - 8.5 6.5 - 8.5 s 7.7

Conductivity (μmhos/cm) - Report 42,890

Temperature °C - Report 19.6

Salinity - - 31.2 c

Total Residual Chlorine (mg/L) ≤ 0.01 - ≤ 0.03 £

Chlorophyll a (μg/L) - Corrected - - 13

Phaeophytin (μg/L) - - 3.3

Total Suspended Solids (mg/L) - Report 4 U

Total Dissolved Solids (mg/L) - - 28,200 A

CBOD, 5 day (mg/L), N - inhibited - - 1.3 AI

Oils and Greases (mg/L) ≤ 5.0 - 1.5 I F 1.4 I *

Chloride (mg/L) - - 15,000

Fluoride (mg/L) ≤ 5.0 ≤ 5.0 d 1.2

Sulfate (mg/L) - - 2,100

Alpha, Total (pCi/L) ≤ 15 - 1.8

Alpha-Counting Error (pCi/L) - - 0.9

Radium 226 (pCi/L) - - 1.1

Radium 226-Counting Error (pCi/L) - - 0.2

Radium 228 (pCi/L) - - 0.8

Radium 228-Counting Error (pCi/L) - - 0.5

Radium 226 + 228 (pCi/L) ≤ 5.0 d ≤ 5.0 d 1.9 c

Flow (MGD) - Report 5.15 a

Hardness (mg CaCO3) - - 5224.05 c

Value exceeds the Class III Water Quality Criteria or permit limits

a - Annual average

c - Value is calculated

d - Daily maximum

s - Single sample

A - Value reported is the mean of two or more determinations

F - Analyte was detected in both sample and field blank

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit

J - Estimated value

U - Material analyzed for but not detected; value reported is the method detection limit

 # - If 10 or fewer samples are taken within a month, the single sample limit is 400 CFU (62-302.530(6), F.A.C.)

£ - Measured and entered in Central Lab tox sheets

* - Material detected in field blanks

Nutrients (mg/L)

General Physical and Chemical Parameters

Table 2. Effluent limits, Class III Criteria for predominantly marine waters and 

chemical data for samples collected from Kinder Morgan Ammonia Facility on July 

28, 2008.

Effluent

Metals (μg/L unless otherwise noted)
6

Appendix A. Chain of Custody Form 
7

Appendix B. Bench Sheets 
8

Appendix B. (continued) 
9

Appendix B. (continued) 
10

Appendix B. (continued) 
11

Appendix B. (continued) 
12

Appendix C. SRT Bench Sheets and Statistical Analysis 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity

Americamysis bahia (mysid shrimp) - Cadmium Chloride (CdCl2) 

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

01/16/07 38.23 54.18 11.66 30.86 77.50

02/13/07 56.06 54.27 11.36 31.55 76.99

03/14/07 70.07 55.86 11.23 33.40 78.32

04/10/07 42.91 56.00 11.04 33.91 78.08

05/08/07 39.00 55.42 11.63 32.16 78.68

05/30/07 49.64 54.95 11.67 31.62 78.29

07/03/07 49.64 55.04 11.62 31.80 78.27

07/31/07 43.54 54.34 11.88 30.58 78.10

09/05/07 45.79 54.15 11.99 30.17 78.13

10/02/07 41.44 53.25 12.24 28.76 77.73

11/06/07 35.65 52.06 12.76 26.55 77.57

12/11/07 50.27 52.86 12.06 28.75 76.98

01/08/08 64.65 53.42 12.03 29.36 77.48

2/5/2008 * 23.52 50.68 13.02 24.64 76.72

02/12/08 36.63 49.45 13.14 23.17 75.74

03/11/08 38.21 47.42 11.38 24.66 70.19

04/01/08 53.27 46.90 10.82 25.26 68.53

05/06/08 35.65 45.82 10.81 24.19 67.45

06/03/08 58.28 46.36 11.17 24.03 68.69

07/08/08 47.67 46.00 10.99 24.02 67.98

Lower Confidence Limit = mean - 2 * Std Dev CV = 23.89

Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 26%

* Test result exceeded control limits on 2/5/2008. Test repeated on 2/12/2008.

Standard Reference Toxicant Data 

Americamysis bahia 48-hour LC50

20

30

40

50

60

70

80

90

01/16/0702/13/0703/14/0704/10/0705/08/0705/30/0707/03/0707/31/0709/05/0710/02/0711/06/0712/11/0701/08/08

2/5/2008 *

02/12/0803/11/0804/01/0805/06/0806/03/0807/08/08

Test Date

μg/L CdCl2

LC50 Mean LC50 Lower Control Limit Upper Control Limit
13

Appendix C. (continued) 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - Acute Toxicity

Menidia beryllina (Inland silverside) - Copper Chloride (CuCl2)

Date LC50 Mean LC50

Lower 

control limit

Lower 

warning limit

Upper 

warning limit

Upper 

control limit

11/28/06 140.31 292.22 201.0 221.3 341.2 391.4

01/16/07 301.29 291.93 201.0 221.3 341.2 391.4

02/14/07 278.00 291.27 201.0 221.3 341.2 391.4

03/14/07 316.16 287.74 201.0 221.3 333.3 391.4

04/10/07 338.35 291.67 201.0 221.3 334.8 391.4

05/22/07 257.44 293.42 201.0 247.5 334.8 391.4

05/30/07 247.36 289.10 201.0 239.9 334.4 391.4

07/03/07 357.65 288.79 201.0 239.9 334.4 391.4

07/31/07 350.68 290.88 201.0 239.9 341.4 391.4

09/05/07 338.18 288.38 201.0 239.9 338.2 363.8

10/02/07 338.90 289.15 201.0 239.9 338.4 363.8

11/06/07 419.55 289.15 201.0 239.9 338.4 363.8

12/04/07 356.07 285.50 201.0 239.9 338.4 356.2

01/08/08 329.03 285.29 200.9 239.9 338.5 356.2

02/05/08 357.47 294.07 210.0 254.9 341.9 357.5

03/04/08 295.52 295.31 210.0 254.9 341.9 357.5

04/01/08 379.50 304.05 216.2 258.5 352.0 359.8

05/06/08 272.50 304.73 216.2 268.7 352.0 359.8

06/03/08 373.47 312.52 243.9 276.6 356.4 347.1

07/08/08 275.20 316.13 256.4 277.3 356.4 347.1

Means are of the last 20 SRT tests.

Upper warning and control limits are the 75th and 90th percentiles, respectively, of the past 20 LC50s.

Lower warning and control limits are the 25th and 10th percentiles, respectively, of the past 20 LC50s.

EPA reference 75th quartile CV = 21% CV = 19.29

Standard Reference Toxicant Data 

Menidia beryllina 48 hour LC50

0

50

100

150

200

250

300

350

400

450

11/28/0601/16/0702/14/0703/14/0704/10/0705/22/0705/30/0707/03/0707/31/0709/05/0710/02/0711/06/0712/04/0701/08/0802/05/0803/04/0804/01/0805/06/0806/03/0807/08/08

Test Date

μg/L CuCl2

Lower warning limit LC50 Mean LC50

Upper warning limit Lower control limit Upper control limit
14

Appendix C. (continued) 
15

Appendix C. (continued) 
16

Appendix C. (continued) 

Americamysis bahia Acute Standard Reference Toxicant Test 

 DATE: 7-8-08 DURATION: 48 hr TOXICANT: CdCl2 

 SPECIES: Americamysis bahia

 RAW DATA: Concentration Number Mortalities 

 (μg/L) Exposed 

 0.00 20 0 

 20.00 20 0 

 30.00 20 1 

 45.00 20 10 

 67.00 20 16 

 100.00 20 20 

 SPEARMAN-KARBER TRIM: .00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 47.67 

 95% LOWER CONFIDENCE: 42.22 

 95% UPPER CONFIDENCE: 53.83 

Menidia beryllina Standard Reference Toxicant Test 

 DATE: 7-8-08 DURATION: 48 hr TOXICANT: CuCl2 

 SPECIES: Menidia beryllina

 RAW DATA: Concentration Number Mortalities 

 (μg/L) Exposed 

 0.00 20 0 

 100.00 20 0 

 200.00 20 3 

 300.00 20 10 

 400.00 20 18 

 500.00 20 20 

 SPEARMAN-KARBER TRIM: .00% 

 SPEARMAN-KARBER ESTIMATES: LC50: 275.20 

 95% LOWER CONFIDENCE: 243.60 

 95% UPPER CONFIDENCE: 310.89 
17

Appendix D. Test Organism Receipts 
18

Appendix D. (continued) 
19

Appendix E. Chemical Analyses performed on the effluent from the Kinder Morgan Ammonia FaD-006, sampled on July 28, 2008.

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:35 Effluent AGP/LN Algal Growth Potential 3 A U mg DryWt/L 7/28/2008 9:35 Effluent Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 1.3 AI mg/L 7/28/2008 9:35 Effluent Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 13 ug/L 7/28/2008 9:35 Effluent Chlorophyll/Grain Size/BOD Phaeophytin-a 3.3 ug/L 7/28/2008 9:35 Effluent Toxicology Bioassay-Acute-Screen-SW-Fish, LC50 100 L LC50 7/28/2008 9:35 Effluent Toxicology Bioassay-Acute-Screen-SW-Mysid, LC50 100 L LC50 7/28/2008 9:35 Effluent Metals Chromium 1.5 U ug/L 7/28/2008 9:35 Effluent Metals Iron 90 U ug/L 7/28/2008 9:35 Effluent Metals Zinc 15 U ug/L 7/28/2008 9:35 Effluent Metals Magnesium 1060 A mg/L 7/28/2008 9:35 Effluent Metals Nickel 0.75 U ug/L 7/28/2008 9:35 Effluent Metals Lead 0.6 U ug/L 7/28/2008 9:35 Effluent Metals Cadmium 0.06 U ug/L 7/28/2008 9:35 Effluent Metals Aluminum 180 U ug/L 7/28/2008 9:35 Effluent Metals Selenium 1.5 U ug/L 7/28/2008 9:35 Effluent Metals Sodium 8590 A mg/L 7/28/2008 9:35 Effluent Metals Arsenic 3 I ug/L 7/28/2008 9:35 Effluent Metals Calcium 344 A mg/L 7/28/2008 9:35 Effluent Metals Silver 0.075 U ug/L 7/28/2008 9:35 Effluent Metals Copper 1.5 UJ ug/L 7/28/2008 9:35 Effluent Nutrients NO2NO3-N 0.004 U mg N/L 7/28/2008 9:35 Effluent Nutrients Fluoride 1.2 mg F/L 7/28/2008 9:35 Effluent Nutrients Kjeldahl Nitrogen 0.75 J mg N/L 7/28/2008 9:35 Effluent Nutrients Sulfate 2100 mg SO4/L 7/28/2008 9:35 Effluent Nutrients Chloride 15000 mg Cl/L 7/28/2008 9:35 Effluent Nutrients O-Phosphate-P 0.22 A mg P/L 7/28/2008 9:35 Effluent Nutrients Ammonia-N 0.023 mg N/L 7/28/2008 9:35 Effluent Nutrients TDS 28200 A mg/L 7/28/2008 9:35 Effluent Nutrients Total-P 0.32 mg P/L 7/28/2008 9:35 Effluent Nutrients TSS 4 U mg/L 7/28/2008 9:35 Effluent Overflow Oil and Grease 1.5 I mg/L 7/28/2008 9:35 Effluent Overflow Radium 228 0.8 pCi/L 7/28/2008 9:35 Effluent Overflow Radium 228-Counting Error 0.5 pCi/L 
20

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:35 Effluent Overflow Alpha, Total 1.8 pCi/L 7/28/2008 9:35 Effluent Overflow Radium 226-Counting Error 0.2 pCi/L 7/28/2008 9:35 Effluent Overflow Alpha-Counting Error 0.9 pCi/L 7/28/2008 9:35 Effluent Overflow Radium 226 1.1 pCi/L 7/28/2008 9:35 Effluent Pesticides Metolachlor 0.51 U ug/L 7/28/2008 9:35 Effluent Pesticides Metribuzin 0.1 U ug/L 7/28/2008 9:35 Effluent Pesticides Mevinphos 0.2 U ug/L 7/28/2008 9:35 Effluent Pesticides Phorate 0.051 U ug/L 7/28/2008 9:35 Effluent Pesticides Ametryn 0.051 U ug/L 7/28/2008 9:35 Effluent Pesticides Bromacil 0.2 U ug/L 7/28/2008 9:35 Effluent Pesticides Fonofos 0.1 U ug/L 7/28/2008 9:35 Effluent Pesticides Hexazinone 0.1 U ug/L 7/28/2008 9:35 Effluent Pesticides Naled 0.81 UJ ug/L 7/28/2008 9:35 Effluent Pesticides Norflurazon 0.15 U ug/L 7/28/2008 9:35 Effluent Pesticides Ethion 0.051 U ug/L 7/28/2008 9:35 Effluent Pesticides Malathion 0.15 U ug/L 7/28/2008 9:35 Effluent Pesticides Metalaxyl 0.25 U ug/L 7/28/2008 9:35 Effluent Pesticides Parathion Ethyl 0.15 U ug/L 7/28/2008 9:35 Effluent Pesticides Parathion Methyl 0.1 U ug/L 7/28/2008 9:35 Effluent Pesticides Chlorpyrifos Ethyl 0.051 U ug/L 7/28/2008 9:35 Effluent Pesticides Ethoprop 0.1 U ug/L 7/28/2008 9:35 Effluent Pesticides Fenamiphos 0.2 U ug/L 7/28/2008 9:35 Effluent Pesticides Alachlor 0.61 U ug/L 7/28/2008 9:35 Effluent Pesticides Atrazine 0.051 U ug/L 7/28/2008 9:35 Effluent Pesticides Azinphos Methyl 0.2 U ug/L 7/28/2008 9:35 Effluent Pesticides Butylate 0.2 U ug/L 7/28/2008 9:35 Effluent Pesticides Simazine 0.051 U ug/L 7/28/2008 9:35 Effluent Pesticides Chlorpyrifos Methyl 0.1 U ug/L 7/28/2008 9:35 Effluent Pesticides Diazinon 0.051 UJ ug/L 7/28/2008 9:35 Effluent Pesticides Prometryn 0.15 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 4-Chlorophenyl phenyl ether 1.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 4-Nitrophenol 14 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Dieldrin 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Endrin aldehyde 3.8 UJ ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.95 U ug/L 
21

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:35 Effluent Priority Organic Pollutants 2,6-Dinitrotoluene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2-Chlorophenol 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Benzidine 95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Benzo(a)anthracene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Benzo(k)fluoranthene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Dimethyl phthalate 9.5 UJ ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Endosulfan I 3.8 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Fluorene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Naphthalene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants delta-BHC 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 4-Chloro-3-methylphenol 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Benzo(g,h,i)perylene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Di-n-butyl phthalate 4.8 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Endosulfan sulfate 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Heptachlor epoxide 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2-Chloronaphthalene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 4,4'-DDD 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Aldrin 1.4 UJ ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Anthracene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Benzo(b)fluoranthene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Chrysene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Endrin 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Hexachlorobenzene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2,4-Dimethylphenol 9.5 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2,4-Dinitrophenol 14 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Acenaphthene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Bis(2-chloroethyl)ether 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Di-n-octyl phthalate 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Diethyl phthalate 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Pyrene 0.95 U ug/L 
22

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:35 Effluent Priority Organic Pollutants gamma-BHC 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 4,4'-DDT 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Benzo(a)pyrene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Butyl benzyl phthalate 4.8 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Fluoranthene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Isophorone 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants N-Nitrosodimethylamine 1.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants alpha-BHC 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2,4-Dichlorophenol 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 3,3'-Dichlorobenzidine 38 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 14 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Dibenzo(a,h)anthracene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Endosulfan II 3.8 UJ ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Heptachlor 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Nitrobenzene 1.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Pentachlorophenol 2.9 UJ ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Phenanthrene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2,4-Dinitrotoluene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Acenaphthylene 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants N-Nitrosodi-n-propylamine 1.9 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants Phenol 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants beta-BHC 1.4 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2,4,6-Trichlorophenol 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 2-Nitrophenol 0.95 U ug/L 7/28/2008 9:35 Effluent Priority Organic Pollutants 4,4'-DDE 1.4 U ug/L 7/28/2008 9:35 Effluent Field Parameter Dissolved Oxygen 6.96 mg/L 7/28/2008 9:35 Effluent Field Parameter Specific Conductance 42890 umhos/cm 7/28/2008 9:35 Effluent Field Parameter pH 7.72 7/28/2008 9:35 Effluent Field Parameter Temperature 19.56 C 7/28/2008 9:45 Blank Metals Iron 30 U ug/L 7/28/2008 9:45 Blank Metals Magnesium 0.04 U mg/L 7/28/2008 9:45 Blank Metals Selenium 0.5 U ug/L 
23

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:45 Blank Metals Silver 0.025 U ug/L 7/28/2008 9:45 Blank Metals Sodium 0.5 U mg/L 7/28/2008 9:45 Blank Metals Zinc 5 U ug/L 7/28/2008 9:45 Blank Metals Copper 0.5 U ug/L 7/28/2008 9:45 Blank Metals Nickel 0.25 U ug/L 7/28/2008 9:45 Blank Metals Arsenic 0.5 U ug/L 7/28/2008 9:45 Blank Metals Aluminum 60 U ug/L 7/28/2008 9:45 Blank Metals Lead 0.2 U ug/L 7/28/2008 9:45 Blank Metals Cadmium 0.02 U ug/L 7/28/2008 9:45 Blank Metals Chromium 0.5 U ug/L 7/28/2008 9:45 Blank Metals Calcium 0.075 U mg/L 7/28/2008 9:45 Blank Nutrients Sulfate 0.2 U mg SO4/L 7/28/2008 9:45 Blank Nutrients Fluoride 0.05 U mg F/L 7/28/2008 9:45 Blank Nutrients Total-P 0.02 U mg P/L 7/28/2008 9:45 Blank Nutrients TSS 4 U mg/L 7/28/2008 9:45 Blank Nutrients Chloride 0.2 U mg Cl/L 7/28/2008 9:45 Blank Nutrients TDS 15 U mg/L 7/28/2008 9:45 Blank Nutrients Ammonia-N 0.01 U mg N/L 7/28/2008 9:45 Blank Nutrients NO2NO3-N 0.004 U mg N/L 7/28/2008 9:45 Blank Nutrients Kjeldahl Nitrogen 0.08 U mg N/L 7/28/2008 9:45 Blank Nutrients O-Phosphate-P 0.004 U mg P/L 7/28/2008 9:45 Blank Overflow Radium 228-Counting Error 0.5 pCi/L 7/28/2008 9:45 Blank Overflow Radium 228 0.8 U pCi/L 7/28/2008 9:45 Blank Overflow Radium 226-Counting Error 0.1 pCi/L 7/28/2008 9:45 Blank Overflow Alpha-Counting Error 0.5 pCi/L 7/28/2008 9:45 Blank Overflow Oil and Grease 1.4 I mg/L 7/28/2008 9:45 Blank Overflow Alpha, Total 0.8 U pCi/L 7/28/2008 9:45 Blank Overflow Radium 226 0.1 U pCi/L 7/28/2008 9:45 Blank Pesticides Fenamiphos 0.19 U ug/L 7/28/2008 9:45 Blank Pesticides Chlorpyrifos Methyl 0.097 U ug/L 7/28/2008 9:45 Blank Pesticides Metalaxyl 0.24 U ug/L 7/28/2008 9:45 Blank Pesticides Mevinphos 0.19 U ug/L 7/28/2008 9:45 Blank Pesticides Phorate 0.049 U ug/L 7/28/2008 9:45 Blank Pesticides Prometryn 0.15 U ug/L 7/28/2008 9:45 Blank Pesticides Alachlor 0.58 U ug/L 
24

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:45 Blank Pesticides Butylate 0.19 U ug/L 7/28/2008 9:45 Blank Pesticides Parathion Methyl 0.097 U ug/L 7/28/2008 9:45 Blank Pesticides Simazine 0.049 U ug/L 7/28/2008 9:45 Blank Pesticides Bromacil 0.19 U ug/L 7/28/2008 9:45 Blank Pesticides Diazinon 0.049 UJ ug/L 7/28/2008 9:45 Blank Pesticides Hexazinone 0.097 U ug/L 7/28/2008 9:45 Blank Pesticides Malathion 0.15 U ug/L 7/28/2008 9:45 Blank Pesticides Metolachlor 0.49 U ug/L 7/28/2008 9:45 Blank Pesticides Naled 0.78 UJ ug/L 7/28/2008 9:45 Blank Pesticides Azinphos Methyl 0.19 U ug/L 7/28/2008 9:45 Blank Pesticides Chlorpyrifos Ethyl 0.049 U ug/L 7/28/2008 9:45 Blank Pesticides Parathion Ethyl 0.15 U ug/L 7/28/2008 9:45 Blank Pesticides Metribuzin 0.097 U ug/L 7/28/2008 9:45 Blank Pesticides Fonofos 0.097 U ug/L 7/28/2008 9:45 Blank Pesticides Ethoprop 0.097 U ug/L 7/28/2008 9:45 Blank Pesticides Ethion 0.049 U ug/L 7/28/2008 9:45 Blank Pesticides Norflurazon 0.15 U ug/L 7/28/2008 9:45 Blank Pesticides Ametryn 0.049 U ug/L 7/28/2008 9:45 Blank Pesticides Atrazine 0.049 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants alpha-BHC 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2,4-Dinitrophenol 15 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2-Chloronaphthalene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 4-Chlorophenyl phenyl ether 2 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Anthracene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Bis(2-chloroisopropyl)ether 2.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Diethyl phthalate 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Fluorene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Hexachlorobutadiene 2.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants delta-BHC 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Benzo(a)pyrene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Di-n-butyl phthalate 4.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Di-n-octyl phthalate 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Hexachloroethane 2.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants N-Nitrosodi-n-propylamine 2 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Nitrobenzene 2 U ug/L 
25

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:45 Blank Priority Organic Pollutants 2,4,6-Trichlorophenol 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2,6-Dinitrotoluene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Aldrin 1.5 UJ ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Endosulfan I 3.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants N-Nitrosodimethylamine 2 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Pyrene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Benzo(k)fluoranthene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Bis(2-chloroethyl)ether 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 15 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Butyl benzyl phthalate 4.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Dimethyl phthalate 9.8 UJ ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Phenol 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2,4-Dimethylphenol 9.8 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 2.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Benzidine 98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Benzo(a)anthracene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Bis(2-chloroethoxy)methane 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Dieldrin 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Endosulfan II 3.9 UJ ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Fluoranthene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Heptachlor epoxide 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Phenanthrene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 1,2,4-Trichlorobenzene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2,4-Dichlorophenol 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2-Chlorophenol 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2-Nitrophenol 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 4,4'-DDE 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 4-Bromophenyl phenyl ether 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Acenaphthylene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Benzo(g,h,i)perylene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Chrysene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Endrin aldehyde 3.9 UJ ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Hexachlorocyclopentadiene 2.9 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Isophorone 0.98 U ug/L 
26

Appendix E. (continued)

Date Sampled Field ID Analysis Group Components Result Remarks Units 

7/28/2008 9:45 Blank Priority Organic Pollutants Naphthalene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Pentachlorophenol 2.9 UJ ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants beta-BHC 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 3,3'-Dichlorobenzidine 39 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 4,4'-DDD 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 4,4'-DDT 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Acenaphthene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Dibenzo(a,h)anthracene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Endrin 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Hexachlorobenzene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants gamma-BHC 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 2,4-Dinitrotoluene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 4-Chloro-3-methylphenol 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants 4-Nitrophenol 15 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Benzo(b)fluoranthene 0.98 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Endosulfan sulfate 1.5 U ug/L 7/28/2008 9:45 Blank Priority Organic Pollutants Heptachlor 1.5 U ug/L 
27

Appendix F. Toxicity Instrumentation 

 FDEP Biology Laboratory - Bioassay Instrument Identification

Test Dates: July 29 - 31, 2008

METER / DEVICE ID SOP Parameter Method √ used

YSI Model 58 Dissolved Oxygen 

Meter 90H018262 TA06_04

Dissolved 

Oxygen SM 4500 O G √

Accumet AR-10 pH Meter 93312105 TA06_23 pH SM 4500 H+ √

Mettler-Toledo SevenMulti 

Conductivity Meter

SN 1225117049 

Probe H04257 TA06_25

Conductivity 

Salinity

SM 2510 B 

SM 2520 B

√ 

√

Denver Model 225 ISE with 

ammonia probe SN K01377 TA06_17 Ammonia SM 4500 NH3 F √

Thermometer (DO probe)

YSI 5750 

SN 05J1720 TA06_05 Temperature

SM-2530B 

modified 

DEP SOP001/01 FT1400 √

Hach Pocket Colorimeter II 05050C026378 TA06_22

Total Residual 

Chlorine

EPA 330.5 

SM 4500 CL G √

Alkalinity (Hach Kit) No SN TA06_08 Alkalinity SM 2320 B √

Hardness (Hach Kit) No SN TA06_09 Hardness SM 2340 C √

OHAUS Balance 250D 1125481084

TA06_21 

TA06_27 Weight - √

S-Weights BIO-S-WEIGHTS-01 NA Balance check - √

SM-2530B modified to allow use electronic and non-mercury filled thermometers.

Insert other device information in blank spaces(if used). Test ID = Facility or SRTs (acute SRT--Chronic SRT)

Comments:

V 1.4 8/21/08 BA

Test ID: Kinder Morgan Ammonia Facility SRT Dates: July 8 - 12, 2008

 
28

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 2 6 4 11 12 0 8 0 7 2 8 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 2 6 4 11 12 0 8 0 7 2 8 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 0 2 6 4 11 12 0 8 0 7 2 8 18 S 19 S 20 2

66

Biological Analyses of Kinder Morgan Ammonia Facility effluent 

sampled on July 28, 2008. NPDES #FL0000264.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of Kinder Morgan Ammonia Facility effluent 

sampled on July 28, 2008. NPDES #FL0000264.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of Kinder Morgan Ammonia 

Facility effluent sampled on July 28, 2008. NPDES #FL0000264.