DRAFT 

Endocrine Disruptor Screening Program 

Contract No: EP-W-11-063 

Task Order No: 2 

Statistical Support for Technical Directive #5 

Data Analysis Support for Avian Tier 2 Test and Generation of Integrated Summary 

Report 

Inter-Laboratory Statistical Analysis 

U.S. Environmental Protection Agency 

May 20, 2013 

 

BATTELLE 

505 King Avenue 

Columbus, Ohio 43201 
i 

Table of Contents 

1. Introduction ...................................................................................................................................... 1 

2. Questions to be addressed. Locations of answers. .......................................................................... 1 

3. Study data ........................................................................................................................................ 3 

4. Inter-laboratory analyses .................................................................................................................. 6 

4.1 Inter-laboratory Analyses of Control Data ........................................................................ 7 

4.2 Comparisons of Vinclozolin and the Control for Each Generation .................................. 8 

4.3 Comparison of Generations .............................................................................................. 8 

5. Results .............................................................................................................................................. 9 

6. Comparison of Inter-laboratory CVs and Within-study Sensitivity across Endpoints ................ 116 

6.1 Comparison of Inter-laboratory CVs across Endpoints ................................................ 116 

6.2 Comparison of Within-study Power Sensitivity across Endpoints ............................... 117 

Appendices 

Appendix A – Figures for Avian Control Data

Appendix B - Figures for Avian Inter-Laboratory Analysis: Comparisons of Vinclozolin vs Control

Tables 

Table 1. Variables and Description .............................................................................................................. 3 

Table 2. Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors 

with Random Laboratory Effect, F0 Generation ........................................................................ 10 

Table 3. Inter-laboratory Analysis for Avian Control Data across All Studies: Variance 

Component Analysis with Random Laboratory, F0 Generation ................................................ 14 

Table 4. Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction 

Intervals within Laboratory and Between Laboratories, F0 Generation ..................................... 18 

Table 5. Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors 

with Random Laboratory Effect, F1 Generation ........................................................................ 22 

Table 6. Inter-laboratory Analysis for Avian Control Data across All Studies: Variance 

Component Analysis with Random Laboratory, F1 Generation ................................................ 28 

Table 7. Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction 

Intervals within Laboratory and Between Laboratories, F1 Generation ..................................... 36 

Table 8. Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors 

with Random Laboratory Effect, F2 Generation ........................................................................ 42 
 

ii 

Table 9. Inter-laboratory Analysis for Avian Control Data across All Studies: Variance 

Component Analysis with Random Laboratory, F2 Generation ................................................ 45 

Table 10. Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction 

Intervals within Laboratory and Between Laboratories, F2 Generation ..................................... 48 

Table 11. Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) 

Significance Level. Avian Study, F0 Generation. ..................................................................... 51 

Table 12. Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) 

Significance Level. Avian Study, F1 Generation. ..................................................................... 54 

Table 13. Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) 

Significance Level. Avian Study, F2 Generation. ..................................................................... 59 

Table 14. Mean and Standard Errors and Comparisons of Avian Vinclozolin Concentrations 

to Control across Laboratories, F0 Generation ........................................................................... 61 

Table 15. Mean and Standard Errors and Comparisons of Avian Vinclozolin Concentrations 

to Control across Laboratories, F1 Generation ........................................................................... 76 

Table 16. Mean and Standard Errors and Comparisons of Avian Vinclozolin Concentrations 

to Control across Laboratories, F2 Generation ......................................................................... 100 

Table 17. Differences between Generations across Laboratories. Chemical Vincolozolin, 

Avian Study. ............................................................................................................................. 109 

Table 18. CV (%) and Significance Levels across Laboratories.............................................................. 118

Table 19. Ranges of CV% across Laboratories. By Category. ............................................................... 121

Table 20. Percent Deviations from Control Group Means for Detections with 80% Power and 

the Ratios Between Gyrations. ................................................................................................. 122 

Table 21. Ranges of Percent Deviations from Control Group Means for Detections with 

80% Power and the Ratios Between Generations. By Category. ............................................ 127 

Figures 

Figure 1. F0 CV% vs. F1 CV% for Corresponding Parameters. Body Weight. ..................................... 128 

Figure 2. F1 CV% vs. F2 CV% for Corresponding Parameters. Body Weight. ..................................... 129 

Figure 3. F0 CV% vs. F1 CV% for Corresponding Parameters. Feed Consumption. ............................ 130 

Figure 4. F0 CV% vs. F1 CV% for Corresponding Parameters. Reproduction. ..................................... 131 

Figure 5. F0 CV% vs. F1 CV% for Corresponding Parameters. Egg Shell and Weight. ....................... 132 

Figure 6. F1 CV% vs. F2 CV% for Corresponding Parameters. Mating Behavior. ............................... 133 

Figure 7. F0 CV% vs. F1 CV% for Corresponding Parameters. Thyroid Weight. ................................. 134 

Figure 8. F1 CV% vs. F2 CV% for Corresponding Parameters. Thyroid Weight. ................................. 135 
 

iii 

Figure 9. F1 CV% vs. F2 CV% for Corresponding Parameters. Sex Ratio. ........................................... 136 

Figure 10. F0 CV% vs. F1 CV% for Corresponding Parameters. Bio Chem. ........................................ 137 

Figure 11. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Body Weight. ................................................ 138 

Figure 12. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Body Weight. ................................................ 139 

Figure 13. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Feed Consumption. ....................................... 140 

Figure 14. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Reproduction. ............................................... 141 

Figure 15. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Egg Shell and Wight. .................................... 142 

Figure 16. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Mating Behavior. .......................................... 143 

Figure 17. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Thyroid Weight. ............................................ 144 

Figure 18. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Thyroid Weight. ............................................ 145 

Figure 19. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Sex Ratio. ..................................................... 146 

Figure 20. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding 

Parameters to Detect Effects with Power 80%. Bio Chem. ..................................................... 147 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

1

1. Introduction 

To evaluate the effects of endocrine disrupting chemicals, EPA is currently developing and 

validating a Tier 2, two-generation avian toxicity test to characterize the adverse consequences of 

exposure to putative endocrine active substances. Tests were carried out by EPA in three laboratories: 

Smithers Viscient LLC (SV), Bayer CropScience Inc (ST), and Wildlife International (WLI). All three 

laboratories carried out tests with vincolozline at 64, 160, 400, and 1000 ppm concentration levels. The 

intra-laboratory analyses were performed for each laboratory data by WLI and were presented in the inlaboratory analysis reports. This report presented only the inter-laboratory analysis as carried out at the 

three laboratories. Individual summary statistics, such as mean and standard errors may be presented in 

this report; however, the significant test results for individual laboratories were not presented nor 

summarized in this report. 

2. Questions to be addressed. Locations of answers. 

EPA requested that inter-laboratory analyses be carried out to answer a series of questions. The 

questions raised by EPA and the report tables that pertain to those questions are given below: 

 Is the behavior of controls similar among labs? 

o What are the ranges of control values within a single laboratory? 

Table 2, Table 3, and Table 4 display the control group mean values (averaged across all 

samples) for each laboratory and pooled across laboratories, as well as the CV% across 

laboratories. The CV% incorporates laboratory-to-laboratory variability. These tables 

corespond to generation F0, F1, and F2, respectively. The means ± 2standard errors of 

means were also displayed in Appendix A. 

Table 5, Table 6, and Table 7 display the within-laboratory variances of the control group 

means within each laboratory, the pooled within-laboratrory variance across laboratories, 

the random laboratory-to-laboratory variance, and the variance for the averages across 

laboratories. One table corresponds to one of the three generations F0, F1, and F2. 

Table 8, Table 9, and Table 10 present within-laboratory 95% prediction intervals that 

give the anticipated range of control group mean values about the overall mean with 95% 

confidence within a single laboratory. One table corresponds to one of the three 

generations F0, F1, and F2. 

o What are the ranges of control values between multiple laboratories? 

Table 8, Table 9, and Table 10 present 95% prediction intervals that give the anticipated 

range of control group mean values about the overall mean with 95% confidence across 

independent laboratories. One table corresponds to one of the three generations F0, F1, 

and F2. 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

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o Are there differences in the technical proficiency (i.e. variability of controls) among 

laboratories? 

Table 5, Table 6, and Table 7 also display the p-values of tests of homogeneity of withinlaboratory variances. This indicates whether there is evidence of differences in the 

within-laboratory variability among laboratories. One table corresponds to one of the 

three generations F0, F1, and F2. 

o What is the power for detecting treatment-related effects based on the variability of 

endpoints measured in controls? 

Table 11, Table 12, and Table 13 display the distances from the control group means and 

the upper and lower alternative values that can be detected with power 80% at 5% 

significance level (two-sided). One table corresponds to one of the three generations F0, 

F1, and F2. 

 Are dose-response relationships similar among labs, in terms of: 

o Sensitivity (chemical concentrations that results in differences between treatment and 

controls): 

Table 14, Table 15, and Table 16 display the means and standard errors for each 

concentration group, within laboratories and pooled across laboratories, and the 

significant differences identified between test group and control group responses for 

vinclozolin pooled across laboratories. Both overall individually significant groups and 

significant trends across concentration groups are indicated, based on the results of 

overall ANOVA F-tests, tests for differences between concentration and control groups, 

and step-down tests for linear concentration trends. The individual laboratory significant 

test results are not presented in this inter-laboratory report, and are presented elsewhere in 

the intra-laboratory report. One table corresponds to one of the three generations F0, F1, 

and F2. These tables can be used to determine the lowest effect concentration as the 

lowest concentration at which a significant difference between test group and control 

group is observed. The means ± 2 standard errors of means were also displayed in 

Appendix B. 

o Slope (requires fitting the same model to data from all labs involved in comparison): 

Table 14, Table 15, and Table 16 also show significant trends across concentration 

groups among all laboratories, when present. Individual slope values were not 

determined. 

 Is there value added to >1 generation (may be within multigenerational studies, between 1 

generation and >1 generation studies)? 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

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Table 17 displays the differences of averages between each of pair of generations F0, F1, 

and F2 and their standard errors for each concentration group, pooled across laboratories. 

This table also displays the summary of the significant differences identified between 

each two generations among the three generations F0, F1, and F2 for vinclozolin, pooled 

across laboratories. Both individual significant groups and significant trends across 

concentration groups are indicated. Table 17 can be used to determine the lowest effect 

concentration as the lowest concentration at which a significant difference between 

generations is observed. The significant results between generations for individual 

laboratory were not presented in this report. 

3. Study data 

The study endpoints that are included in the inter-laboratory analyses are the same as those that 

were included in the intra-laboratory analyses. 

The test method specifies various endpoints (variables) as listed in Table 1. Some endpoints were 

recorded for more than one generation, while others were only recorded in one generation. These 

endpoints are divided among 9 categories. The study data were provided to Battelle in CVS (comma 

separated values) formats by WLI, and the data files names were also listed in Table 1. The data values 

for each endpoint can be found in the corresponding data files. 

Table 1. Variables and Description 

Variable 

Name Description of Variable Data Filesa

 Generation

Body Weight 

bwm0 Adult body weight - males (g) Day 0 X1_XX_abwm.csv F0, F1 

bwm56 Adult body weight - males (g) Final X1_XX_abwm.csv F0, F1 

bwF0 Adult body weight - females (g) Day 0 X1_XX_abwF.csv F0, F1 

bwF56 Adult body weight - females (g) Final X1_XX_abwF.csv F0, F1 

offbw0 Mean offspring (F1 & F2) body weight by pen (g) Day 0 X1_XX_OffBW.csv F1, F2 

offbw14 Mean offspring (F1 & F2) body weight by pen (g) Day 14 X1_XX_OffBW.csv F1, F2 

Feed Consumption 

AdultFC1 Adult feed consumption (g/bird/day) - Week 1 X2_XX_AdultFC.csv F0, F1 

AdultFC2 Adult feed consumption (g/bird/day) - Week 2 X2_XX_AdultFC.csv F0, F1 

AdultFC3 Adult feed consumption (g/bird/day) - Week 3 X2_XX_AdultFC.csv F0, F1 

AdultFC4 Adult feed consumption (g/bird/day) - Week 4 X2_XX_AdultFC.csv F0, F1 

AdultFC5 Adult feed consumption (g/bird/day) - Week 5 X2_XX_AdultFC.csv F0, F1 

AdultFC6 Adult feed consumption (g/bird/day) - Week 6 X2_XX_AdultFC.csv F0, F1 

AdultFC7 Adult feed consumption (g/bird/day) - Week 7 X2_XX_AdultFC.csv F0, F1 

AdultFC8 b Adult feed consumption (g/bird/day) - Week 8 X2_XX_AdultFC.csv F0, F1 

AdultFC9 c Adult feed consumption (g/bird/day) - Week 9 X2_XX_AdultFC.csv F0, F1 

AdultFC10 Adult feed consumption (g/bird/day) - Week 10 X2_SV_AdultFC.csv F1 

AdultFC11 Adult feed consumption (g/bird/day) - Week 11 X2_SV_AdultFC.csv F1 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

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Reproduction 

Laid Eggs Laid X3_XX_Repro.csv F0, F1 

Crack Eggs Crack X3_XX_Repro.csv F0, F1 

Abnormal Eggs Abnormal X3_XX_Repro.csv F0, F1 

FGSet FG Eggs Set X3_XX_Repro.csv F0, F1 

EFGSet EFG Eggs Set X3_XX_Repro.csv F0, F1 

FGFert FG Eggs Fertile X3_XX_Repro.csv F0, F1 

EFGFert EFG Eggs Fertile X3_XX_Repro.csv F0, F1 

FGViable FG Eggs Viable X3_XX_Repro.csv F0, F1 

EFGVia EFG Eggs Viable X3_XX_Repro.csv F0, F1 

FG2Wk FG 2Wk Via X3_XX_Repro.csv F0, F1 

EFG2Wk EFG 2Wk Via X3_XX_Repro.csv F0, F1 

FGHatch FG Hatch X3_XX_Repro.csv F0, F1 

FG14Day FG 14-Day Survival X3_XX_Repro.csv F0, F1 

Laid_Hen_Day Laid / Hen / Day (49) X3_XX_Repro.csv F0, F1 

Crack_Laid Crack / Laid X3_XX_Repro.csv F0, F1 

Abnorm_Laid Abnormal / Laid X3_XX_Repro.csv F0, F1 

FGFert_Set F&G Fert / F&G Set X3_XX_Repro.csv F0, F1 

EFGFert_Set EF&G Fert / EF&G Set X3_XX_Repro.csv F0, F1 

FGVia_Set F&G Viable / F&G Set X3_XX_Repro.csv F0, F1 

EFGVia_Set EF&G Viable / EF&G Set X3_XX_Repro.csv F0, F1 

FG2Wk_Via F&G 2-Wk / F&G Viable X3_XX_Repro.csv F0, F1 

EFG2Wk_Via EF&G 2-Wk / EF&G Viable X3_XX_Repro.csv F0, F1 

FGHatch_2Wk F&G Hatch / F&G 2-Wk X3_XX_Repro.csv F0, F1 

FG14Day_Hatch F&G 14-Day / F&G Hatch X3_XX_Repro.csv F0, F1 

FGHatch_Set F&G Hatch / F&G Set X3_XX_Repro.csv F0, F1 

FGHatch_Fert F&G Hatch / F&G Fert X3_XX_Repro.csv F0, F1 

FG14Day_Set F&G 14-Day / F&G Set X3_XX_Repro.csv F0, F1 

FGHatch_H_D F&G Hatch / Hen / Day (14) X3_XX_Repro.csv F0, F1 

FG14Day_H_D F&G 14-Day / Hen / Day (14) X3_XX_Repro.csv F0, F1 

Egg Shell and Weight

EggShellThick Pen mean Egg Shell Thickness (mm) X4_XX_EggShell_Wt.csv F0, F1 

EggWtAlb Pen mean Egg Content Weight - Albumin (g) X4_XX_EggShell_Wt.csv F0, F1 

EggWtYolk Pen mean Egg Content Weight - Yolk (g) X4_XX_EggShell_Wt.csv F0, F1 

EggWtComb Pen mean Egg Contents Weight - Combined (g) X4_XX_EggShell_Wt.csv F0, F1 

First Foam First Egg Cloacal Size 

AgeFoam Age in days at first foam - males X5_XX_AgeFoamEgg.csv F1 

AgeEgg Age in days at first egg - females X5_XX_AgeFoamEgg.csv F1 

CloacaM4d Cloacal area (cm2) at 4 weeks - Males X5_XX_CloacaMale.csv F1 

CloacaM5d Cloacal area (cm2) at 5 weeks - Males X5_XX_CloacaMale.csv F1 

CloacaM6d Cloacal area (cm2) at 6 weeks - Males X5_XX_CloacaMale.csv F1 

CloacaM7 Cloacal area (cm2) at 7 weeks - Males X5_WIL_CloacaMale.csv F1 

CloacaM8 Cloacal area (cm2) at 8 weeks - Males X5_WIL_CloacaMale.csv F1 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

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CloacaM9 Cloacal area (cm2) at 9 weeks - Males X5_WIL_CloacaMale.csv F1 

CloacaF4e Cloacal area (cm2) at 4 weeks - Females X5_XX_CloacaFemale.csv F1 

CloacaF5e Cloacal area (cm2) at 5 weeks - Females X5_XX_CloacaFemale.csv F1 

CloacaF6e Cloacal area (cm2) at 6 weeks - Females X5_XX_CloacaFemale.csv F1 

CloacaF7 Cloacal area (cm2) at 7 weeks - Females X5_WIL_CloacaFemale.csv F1 

CloacaF8 Cloacal area (cm2) at 8 weeks - Females X5_WIL_CloacaFemale.csv F1 

CloacaF9 Cloacal area (cm2) at9 weeks - Females X5_WIL_CloacaFemale.csv F1 

Mating Behaviorg

NeckGrab1 Length of time to first neck grab (sec) - Day 1 X6_XX_Neck_Grab.csv F1, F2 

NeckGrab2 Length of time to first neck grab (sec) - Day 2 X6_XX_Neck_Grab.csv F1, F2 

NeckGrab3 Length of time to first neck grab (sec) - Day 3 X6_XX_Neck_Grab.csv F1, F2 

ChangeNGf Change in length of time to first neck grab (sec) X6_XX_Neck_Grab.csv F1, F2 

Mount1 Length of time to first mount (sec) - Day 1 X6_XX_Mount.csv F1, F2 

Mount2 Length of time to first mount (sec) - Day 2 X6_XX_Mount.csv F1, F2 

Mount3 Length of time to first mount (sec) - Day 3 X6_XX_Mount.csv F1, F2 

ChangeMtf Change in length of time to first mount (sec) X6_XX_Mount.csv F1, F2 

Attempts1 Number of mount attempts (count) - Day 1 X6_XX_NAttempts.csv F1, F2 

Attempts2 Number of mount attempts (count) - Day 2 X6_XX_NAttempts.csv F1, F2 

Attempts3 Number of mount attempts (count) - Day 3 X6_XX_NAttempts.csv F1, F2 

ChangeAtf Change in number of mount attempts (count) X6_XX_NAttempts.csv F1, F2 

CloCon1 Number of cloacal contacts (count) - Day 1 X6_XX_CloacalCon.csv F1, F2 

CloCon2 Number of cloacal contacts (count) - Day 2 X6_XX_CloacalCon.csv F1, F2 

CloCon3 Number of cloacal contacts (count) - Day 3 X6_XX_CloacalCon.csv F1, F2 

ChangeCCf Change in number of cloacal contacts (count) X6_XX_CloacalCon.csv F1, F2 

CrowFluff1 Number of crows (cock) or fluffs (hen) (count) - Day 1 X6_XX_CrowFluff.csv F1, F2 

CrowFluff2 Number of crows (cock) or fluffs (hen) (count) - Day 2 X6_XX_CrowFluff.csv F1, F2 

CrowFluff3 Number of crows (cock) or fluffs (hen) (count) - Day 2 X6_XX_CrowFluff.csv F1, F2 

ChangeCFf Change in number of crows (cock) or fluffs (hen) (count) X6_XX_CrowFluff.csv F1, F2 

Thyroid Weight 

ThyF214DayM 14-Day Old left thyroid weight (mg) – Males X5_WIL_14DayThy.csv 

X7_SV_14DayThy.csv F2 

ThyF214DayF 14-Day Old left thyroid weight (mg) - Females X5_WIL_14DayThy.csv 

X7_SV_14DayThy.csv F2 

ThyF214Day 14-Day Old left thyroid weight (mg) h X5_ST_14DayThy.csv F2 

ThyWtED15 15 Day Embryo (ED15) Thyroid Weights (mg) X7_XX_ED15Thy.csv F1, F2 

ThyAdultMale Adult left thyroid weight (mg) - Males X7_XX_AdultThy.csv F0, F1 

ThyAdultFemale Adult left thyroid weight (mg) - Females X7_XX_AdultThy.csv F0, F1 

Sex Ratio 

SexRatio Ratio of number of males to total number of offspring (%) X8_XX_SexRatio.csv F1, F2 

Bio Chem 

YolkE2 Egg Yolk Estradiol (pg/g) X9_XX_YolksE2.csv F0, F1 

YolkT Egg Yolk Testosterone (pg/g) X9_XX_YolksT.csv F0, F1 

AmT3 Plasma Thyroid Hormone T3 - Adult Males (ng/mL) X9_XX_AdultMaleT3.csv F0, F1 

AfT3 Plasma Thyroid Hormone T3 -Adult Females (ng/mL) X9_XX_AdultFemaleT3.csv F0, F1 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

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Day14mT3 Plasma Thyroid Hormone T3 -14-Day Old Males (ng/mL) X9_XX_14DayMaleT3.csv F2 

Day14fT3 Plasma Thyroid Hormone T3 -14-Day Old Females (ng/mL) X9_XX_14DayFemaleT3.csv F2 

AmT4 Plasma Thyroid Hormone T4 -Adult Males (ng/mL) X9_XX_AdultMaleT4.csv F0, F1 

AfT4 Plasma Thyroid Hormone T4 -Adult Females (ng/mL) X9_XX_AdultFemaleT4.csv F0, F1 

Day14mT4 Plasma Thyroid Hormone T4 -14-Day Old Males (ng/mL) X9_XX_14DayMaleT4.csv F2 

Day14fT4 Plasma Thyroid Hormone T4 -14-Day Old Females (ng/mL) X9_XX_14DayFemaleT4.csv F2 

AmE2 Plasma Sex Hormone Estradiol - Adult Males (pg/mL) X9_XX_AdultMaleE2.csv F0, F1 

AfE2 Plasma Sex Hormone Estradiol - Adult Females (pg/mL) X9_XX_AdultFemaleE2.csv F0, F1 

Day14mE2 Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) X9_XX_14DayMaleE2.csv F2 

Day14fE2 Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) X9_XX_14DayFemaleE2.csv F2 

AmT Plasma Sex Hormone Testosterone - Adult Males (pg/mL) X9_XX_AdultMaleT.csv F0, F1 

AfT Plasma Sex Hormone Testosterone - Adult Females (pg/mL) X9_XX_AdultFemaleT.csv F0, F1 

Day14mT Plasma Sex Hormone Testosterone -14-Day Old Males (pg/mL) X9_XX_14DayMaleT.csv F2 

Day14fT Plasma Sex Hormone Testosterone -14-Day Old Females (pg/mL) X9_XX_14DayFemaleT.csv F2 

a. XX – Laboratory ID: ST, SV or WIL, unless otherwise noted. 

b. Laboratory ST has only generation F1 data for AdultFC8. 

c. Laboratory ST has only generation F1 data for AdultFC9. Laboratory WIL has no data for AdultFC9. 

d. Measurements for CloacaM4, CloacaM5, and CloacaM6 were recorded in mm2

 for Laboratory ST. They were 

converted to cm2 to be consistent with other two laboratories in the inter-laboratory analysis. 

e. Measurements for CloacaF4, CloacaF5, and CloacaF6 were recorded in mm2

 for Laboratory ST. They were converted 

to cm2 to be consistent with other two laboratories in the inter-laboratory analysis. 

f. Variables ChangeNG, ChangeMt, ChangeAt, ChangeCC, and ChangeCF were renamed to NeckChange, MountChange, 

AttChange, CloChange, and CrowChange, respectively, in the inter-laboratory analysis. 

g. Laboratory ST had only generation F1 data for mating behavior endpoints, while Laboratories SV and WIL had both 

generations F1 and F2 data for mating behavior endpoints. 

h. Laboratories SV and WIL recorded generation F2 14-Day Old left thyroid weight (mg) separately for males and 

females, while Laboratory ST did not separate this measurement by sex. 

 These endpoints were analyzed across laboratories and their analysis results were discussed in 

this report. This inter-laboratory report did not involve any data handling, except for unit conversion as 

listed in the footnotes under the above table. Data handing, such as the handling of data with “N/D” entry 

for below detection limits, had been conducted by the individual laboratory or by the WLI, which 

performed the intra-laboratory analysis. Any necessary data changes have been included in the CSV data 

files that were sent to Battelle. These data files were read into SAS and used in this inter-laboratory 

analysis. 

4. Inter-laboratory analyses 

The inter-laboratory analysis is composed of three parts. The first part pertains to the comparison 

of the control group results among the three laboratories to assess the range of variation within laboratory 

and among laboratories and the power analyses based on the control groups to detect alternatives from the 

control groups within a laboratory that can be detected with high power, based on average withinlaboratory variability. The second part pertains to the comparisons of the treatment group and control 

group results across laboratories. The third part pertains to the comparisons of generations across 

laboratories. For endpoints with only valid data available for a single laboratory, the inter-laboratory 

analysis was not performed. 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

7

4.1 Inter-laboratory Analyses of Control Data 

Comparisons among control groups were carried out for the F0, F1, and F2 generation results 

separately. The variation in control results was compared among laboratories. Heterogeneous variance 

random effects analysis of variance models with random laboratory effects were fitted to the control 

results from each of the three laboratories, where heterogeneous within-laboratory variance were assumed. 

The averages across the three laboratories were then computed based on the model fits. 

The individual laboratory means, standard errors of means, and within-laboratory variances are 

the unweighted results, as calculated across all the samples within the laboratory, and the averages mean 

and standard error are the model-based least square estimates. Variances for the averages were calculated 

as the square of the standard error. Degrees of freedom for the average across laboratories were estimated 

as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the random laboratory variance, Si

2

and dfi are the reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

,

and K is the total number of laboratories (Hartung and Makambi, 20011

). Pooled within-laboratory 

variance was determined as the unweighted averages of the individual within-laboratory variances with 

degrees of freedom based on Satterthwaite’s approximation. The laboratory-to-laboratory variation was 

estimated by the random effect model. 

The average variance, the pooled within-laboratory variation, and random laboratory variation 

were used to construct 95% two-sided prediction intervals about the average within the control group 

mean. Two levels of prediction intervals were constructed: 

 Within-laboratory variation 

avg 0.975, wi Varavg Varwi ˆ  t  

 Laboratory-to-laboratory variation 

avg 0.975, tot Varavg Varwi Varlab ˆ  t   

Varavg is the variance of the average response pooled across laboratries. Varwi is the withinlaboratory variance. Varlab is the laboratory variance. Degrees of freedom νwi, νlab, and νtotal were 

determined by Satterthwaite’s method. The prediction intervals estimate the ranges of control group 

mean values that may be expected with 95% confidence: within a laboratory and among laboratories. 

Power determinations were carried out for each endpoint based on the control group within run 

variance. Alternatives that can be distinguished from the control group mean with power 80% with a 5% 

(two-sided) significance level within a laboratory were determined. These alternatives are based on the 

non-central t-distribution with noncentrality parameter |δ|/(2swi) where swi is the pooled within labaratory 

standard error, and δ is the deviation from the control, to be determined. Degrees of freedom are twice 

that for the control group and vary with endpoint. Table 11, Table 12, and Table 13 display the control 

group means, the control group nominal sample sizes, and the generation distances from the control group 

mean that can be detected with power 80%; each table presents the results for a generation. 

 

1

 Hartung, J. and Makambi, K.H. (2001), Simple non-iterative t-distribution based tests for meta-analysis, 

South African Statist. J., 35, 1-17. 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

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4.2 Comparisons of Vinclozolin and the Control for Each Generation 

The inter-laboratory analysis output includes numerous tables and figures that display numerous 

summary characteristics for each endpoint. Table 14, Table 15, and Table 16 pertain to comparisons of 

vinclozolin and the controls across laboratories. In these tables, the mean and standard error of means of 

each concentration groups within each individual laboratory were calculated by averaging all sample data 

in the concentration group within the laboratory. The concentration groups means and standard errors 

across the three laboratories were the model-based estimates. 

To compare the vinclozolin concentration groups to the control across laboratories, a 

heterogeneous variance random effects analysis of variance model with random laboratory effect and 

random group within-laboratory effect were fitted to the concentration groups mean results from each of 

the three laboratories, where heterogeneous within group variances of each laboratory were assumed. The 

concentration group was a fixed effect in the model. The average for each concentration group across the 

three laboratories was then computed based on the model fits. Degrees of freedom for the average across 

laboratories were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total 

random variance, as the sum of the random laboratory and random laboratory by concentration variances, 

Si

2

 and dfi are the reported variance and degrees of freedom for a combination of laboratory and 

concentration group, var(SR

2

) is the variance of SR

2

, and K is the number of combinations of laboratory 

and concentration groups (Hartung and Makambi, 2001). The concentration trend tests were performed 

within the fitted model following a step-down fashion: if the linear concentration trend including all five 

concentration groups is significant, then the linear concentration trend after excluding the highest 

concentration groups is tested; following this procedure, the linear concentration trend test after excluding 

the highest concentration group among the remain groups was performed until the linear concentration 

trend is first found to be not significant, then stops. Also, if the overall concentration effect is significant, 

then concentration groups of vinclozolin were each compared to the control to identify which 

concentration groups were significantly different from the control using the Dunnett’s tests. Significant 

differences of vinclozolin concentration group from the control were also indentified using unadjusted ttests. 

 The comparisons of vinclozolin concentration to the control were performed across the three 

laboratories for each generation, and the results were presented in Table 14, Table 15, and Table 16 

separately for each generation. 

4.3 Comparison of Generations 

The comparisons of the generations were performed for each two generations separately for those 

endpoints where data were measured for the two generation to be compared. For each two generations, a 

heterogeneous variance random effects analysis of variance model with laboratory, laboratory by 

generation effect, and laboratory by generation by concentration groups as random effect was fitted to the 

concentration groups mean results from each of the three laboratories for the two generations to be 

compared, where heterogeneous within group variance of a laboratory for a generation were assumed. 

The generation, group, and group by generation were fixed effect in the model. The average of 

differences between the two generations for each conentration group across the three laboratories was 

then computed based on the model fits. Degrees of freedom for the average across laboratories were 

estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

9

as the sum of all random effects in the model, Si

2

 and dfi are the reported variance and degrees of freedom 

for a combination of generation, laboratory, and concentration group, var(SR

2

) is the variance of SR

2

, and 

K is the number of combinations of generation, laboratory, and concentration group (Hartung and 

Makambi, 2001). Differences of the means between the two generations along with the associated 

standard errors were estimates based on the model fits. The concentration trend tests were performed in 

the differences between the two generations within the fitted model following a step-down fashion similar 

to that described in Section 4.2. Also if the overall generation effect or generation by concentration group 

effect is significant, then comparisons of each two generations at each concentration group were 

performed to identify those concentration groups where the two generations were significantly different. 

The differences and their standard errors between each two generations were estimated based on 

the model fits and presented in Table 17. The significant results were also presented in the table. 

5. Results 

Comparisons between the vinclozolin and control showed that: 

 A decreasing concentration trend at the highest concentration level (1000 ppm) is 

observed for Plasma Thyroid Hormone T3 in Adult Females of generation F0 (Table 2). 

 An increasing concentration trend at the highest concentration level (1000 ppm) is 

observed for Plasma Sex Hormone Testosterone in 14-Day Old Females of generation 

F2 (Table 8). 

The differences between the generations are: 

 Adult Body Weight in females on day 0 were significantly lower in F1 generation than in 

F0 generation in all four vinclozolin concentration groups and control group. 

 The differences between generations F1 and F2 in Change in Length of Time to First 

Neck Grab (sec) from Day 1 to Day 3 have a significant decreasing concentration trend at 

the highest concentration level (a significantly negative slope when all five concentration 

groups are included). 

 The differences between generations F1 and F2 in Change in Length of Time to First 

Mount (sec) from Day 1 to Day 3 have a significantly decreasing concentration trend at 

the highest concentration level (a significantly negative slope when all five concentration 

groups are included). 

 The differences between generations F0 and F1 in Plasma Thyroid Hormone T3 in Adult 

Females have a significantly increasing concentration trend at the three highest 

concentration levels (a significantly positive slope when at least the first three 

concentration groups are included). In particular, generation F1 is significantly higher 

than F0 in concentration groups 3 and 4. 
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Table 2. Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Random Laboratory 

Generationd

Parameter 

Individual Laboratory Mean (Standard Error)a Average Acros

Lab1_ST Lab2_SV Lab3_WLI 

Mean 

(Standard Error) b 

Body Weight 

Adult Body Weight (g) Day 0 - Males 190.661 (3.360) 202.739 (4.357) 113.444 (1.828) 168.849 (28.009) 

Adult Body Weight (g) Day 56 - Males 210.625 (3.920) 221.482 (3.807) 122.059 (1.657) 184.637 (31.530) 

Adult Body Weight (g) Day 0 - Females 233.567 (6.594) 247.556 (5.955) 143.222 (2.529) 207.898 (32.810) 

Adult Body Weight (g) Day 56 - Females 249.444 (5.737) 276.924 (6.406) 157.353 (2.598) 227.716 (36.232) 

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 29.611 (0.600) 31.033 (0.722) 18.556 (0.706) 26.402 (3.941) Adult Feed Consumption (g/bird/day) - Week 2 29.667 (0.828) 28.278 (1.198) 20.765 (0.694) 26.199 (2.794) Adult Feed Consumption (g/bird/day) - Week 3 28.944 (0.782) 27.556 (1.022) 19.235 (0.433) 25.203 (3.057) Adult Feed Consumption (g/bird/day) - Week 4 30.824 (1.340) 28.524 (0.693) 21.294 (0.452) 26.791 (2.883) Adult Feed Consumption (g/bird/day) - Week 5 33.438 (1.235) 31.418 (0.696) 22.882 (0.469) 29.183 (3.249) Adult Feed Consumption (g/bird/day) - Week 6 35.750 (1.078) 29.382 (0.708) 21.941 (0.458) 28.979 (3.987) Adult Feed Consumption (g/bird/day) - Week 7 32.375 (0.758) 30.282 (0.674) 22.941 (0.650) 28.524 (2.862) Adult Feed Consumption (g/bird/day) - Week 8 NA 30.076 (0.625) 23.647 (0.477) 26.849 (3.215) Adult Feed Consumption (g/bird/day) - Week 9 NA 34.418 (0.838) NA NA 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 

d. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were av
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Table 2. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F0 Generation 

Parameter 

Individual Laboratory Mean (Standard Error)a Average 

Lab1_ST Lab2_SV Lab3_WLI 

Mean 

(Standard Error) b 

Reproduction 

Eggs Laid 39.750 (1.445) 44.056 (2.509) 44.941 (0.949) 42.897 (1.756) 

Eggs Crack 1.500 (0.532) 1.611 (0.776) 0.118 (0.118) 0.907 (0.540) 

Eggs Abnormal 1.938 (0.710) 2.444 (1.161) 0.235 (0.235) 1.269 (0.725) 

Eggs Set From Weeks 7 and 8 10.438 (0.626) 11.941 (0.397) 12.235 (0.182) 11.678 (0.493) 

Eggs Set From Weeks 6 7 and 8 15.375 (0.957) 18.059 (0.532) 18.294 (0.306) 17.440 (0.830) 

Eggs Fertile From Weeks 7 and 8 9.813 (0.634) 11.706 (0.435) 11.941 (0.303) 11.249 (0.628) 

Eggs Fertile From Weeks 6 7 and 8 14.375 (0.999) 17.824 (0.570) 17.941 (0.415) 16.867 (1.087) 

Eggs Viable From Weeks 7 and 8 9.188 (0.621) 11.647 (0.428) 11.706 (0.318) 10.915 (0.794) 

Eggs Viable From Weeks 6 7 and 8 13.000 (0.979) 17.647 (0.555) 17.647 (0.461) 16.202 (1.494) 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 9.188 (0.621) 11.529 (0.412) 11.588 (0.333) 10.841 (0.752) 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 13.000 (0.979) 17.529 (0.550) 17.471 (0.478) 16.106 (1.444) 

Eggs Hatched 7.750 (0.668) 10.882 (0.469) 10.941 (0.466) 9.907 (1.029) 

Number of 14-Day-Old Surviving Chicks 7.688 (0.663) 9.706 (0.400) 8.706 (0.835) 8.790 (0.639) 

Eggs Laid Per Hen Per Day 0.811 (0.029) 0.980 (0.033) 0.917 (0.019) 0.902 (0.048) 

Cracked Eggs as A Percentage of Eggs Laid 0.039 (0.013) 0.037 (0.019) 0.003 (0.003) 0.022 (0.013) 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 
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Table 2. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F0 Generation 

Parameter 

Individual Laboratory Mean (Standard Error)a Averag

Lab1_ST Lab2_SV Lab3_WLI 

Mean 

(Standard Erro

Reproduction (Continued) 

Abnormal Eggs as A Percentage of Eggs Laid 0.055 (0.022) 0.056 (0.028) 0.005 (0.005) 0.032 (0.019

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.938 (0.022) 0.979 (0.012) 0.976 (0.020) 0.969 (0.011

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.929 (0.022) 0.986 (0.008) 0.980 (0.014) 0.970 (0.015

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.883 (0.031) 0.975 (0.013) 0.957 (0.021) 0.944 (0.026

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.847 (0.034) 0.977 (0.010) 0.964 (0.018) 0.935 (0.039

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as A Percentage 

of Viable Eggs From Weeks 7 and 8 1.000 (0.000) 0.991 (0.006) 0.990 (0.007) 0.995 (0.004

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 6 7 and 8 1.000 (0.000) 0.994 (0.004) 0.990 (0.006) 0.996 (0.003

Eggs Hatched as A Percentage of Number of Viable Eggs from Weeks 7 and 8 

after Two Weeks of Incubation 0.834 (0.035) 0.942 (0.020) 0.940 (0.025) 0.910 (0.033

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs Hatched 0.993 (0.007) 0.896 (0.016) 0.771 (0.062) 0.899 (0.060

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 and 8 0.739 (0.042) 0.909 (0.020) 0.895 (0.037) 0.852 (0.053

Eggs Hatched as A Percentage of Number of Fertile Eggs from Weeks 7 and 8 0.785 (0.039) 0.929 (0.021) 0.916 (0.030) 0.881 (0.044

Number of 14-Day-Old Surviving Chicks as A Percentage of Number of Eggs Set 

from Weeks 7 and 8 0.733 (0.041) 0.814 (0.021) 0.711 (0.067) 0.768 (0.035

Eggs Hatched Per Hen Per Day 0.554 (0.048) 0.778 (0.034) 0.782 (0.033) 0.708 (0.074

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.549 (0.047) 0.692 (0.029) 0.622 (0.060) 0.627 (0.045

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 
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Table 2. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F0 Generation 

Parameter 

Individual Laboratory Mean (Standard Error) a Average 

Lab1_ST Lab2_SV Lab3_WLI 

Mean 

(Standard Error) b 

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.206 (0.002) 0.205 (0.004) 0.234 (0.004) 0.215 (0.009) 

Pen Mean Egg Content Weight - Albumin (g) 5.761 (0.192) 3.913 (0.178) 4.565 (0.112) 4.743 (0.537) 

Pen Mean Egg Content Weight - Yolk (g) 3.660 (0.136) 4.065 (0.171) 3.257 (0.062) 3.640 (0.234) 

Pen Mean Egg Content Weight - Combined (g) 9.421 (0.287) 7.978 (0.316) 7.823 (0.148) 8.394 (0.507) 

Thyroid Weight 

Adult Left Thyroid Weight (mg) - Males 6.113 (0.830) 11.800 (1.407) 4.641 (0.407) 7.348 (2.114) 

Adult Left Thyroid Weight (mg) - Females 10.138 (0.897) 11.362 (1.150) 6.140 (0.535) 9.096 (1.610) 

Bio Chem 

Egg Yolk Estradiol (pg/g) 17.934 (2.934) 47.766 (4.137) 68.882 (6.611) 44.421 (14.757) 

Egg Yolk Testosterone (pg/g) 2077.057 (331.443) 2685.199 (698.976) 1812.517 (393.307) 2050.636 (238.269) 

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.497 (0.080) 1.016 (0.171) 1.131 (0.096) 0.871 (0.204) 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.658 (0.088) 1.030 (0.059) 0.869 (0.094) 0.860 (0.110) 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 3.764 (1.235) 3.178 (0.675) 3.109 (0.364) 3.164 (0.310) 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 1.910 (0.548) 3.328 (1.066) 1.138 (0.237) 1.763 (0.519) 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 4.988 (0.531) 3.796 (0.605) 6.042 (1.501) 4.611 (0.540) 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 12.022 (1.721) 18.032 (3.786) 25.273 (1.824) 18.452 (4.099) 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 1242.501 (317.745) 1151.095 (184.708) 2942.393 (518.354) 1694.232 (538.020) 

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 327.461 (63.129) 317.664 (185.839) 606.982 (293.482) 337.621 (58.572) 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 
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 Table 3. Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component Analysis with 

F0 Generationf

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Variance 

Average/

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Body Weight 

Adult Body Weight (g) Day 0 - Males 11.288/17.0 18.986/17.0 3.341/17.0 11.205/38.5 2342.263(<0.001) 784.484/2

Adult Body Weight (g) Day 56 - Males 15.366/15.0 14.493/16.0 2.746/16.0 10.868/36.2 2971.612(<0.001) 994.156/2

Adult Body Weight (g) Day 0 - Females 43.486/17.0 35.463/17.0 6.396/17.0 28.448/38.8 3201.087(<0.001) 1076.486/

Adult Body Weight (g) Day 56 - Females 32.908/15.0 41.031/16.0 6.750/16.0 26.896/36.1 3911.403(<0.001) 1312.748/

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 0.360/17.0 0.521/17.0 0.498/17.0 0.460/49.8 46.140(<0.001) 15.533/2Adult Feed Consumption (g/bird/day) - Week 2 0.686/17.0 1.436/17.0 0.482/16.0 0.868/41.5 22.560(<0.001) 7.807/2Adult Feed Consumption (g/bird/day) - Week 3 0.611/17.0 1.044/17.0 0.188/16.0 0.614/38.5 27.430(<0.001) 9.347/2Adult Feed Consumption (g/bird/day) - Week 4 1.796/16.0 0.480/16.0 0.204/16.0 0.827/28.1 24.132(<0.001) 8.313/2Adult Feed Consumption (g/bird/day) - Week 5 1.525/15.0 0.484/16.0 0.220/16.0 0.743/28.8 30.931(<0.001) 10.555/2Adult Feed Consumption (g/bird/day) - Week 6 1.163/15.0 0.501/16.0 0.209/16.0 0.624/32.3 47.072(<0.001) 15.898/2Adult Feed Consumption (g/bird/day) - Week 7 0.574/15.0 0.454/16.0 0.423/16.0 0.483/45.7 24.088(<0.001) 8.190/2Adult Feed Consumption (g/bird/day) - Week 8 NA 0.391/16.0 0.228/16.0 0.309/29.9 20.360(<0.001) 10.334/1Adult Feed Consumption (g/bird/day) - Week 9 NA 0.703/16.0 NA NA NA NA 

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 

f. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were ava
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Table 3. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F0 Generation

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Varia

Avera

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Reproduction 

Eggs Laid 2.088/15.0 6.297/17.0 0.901/16.0 3.095/32.2 6.643(0.030) 3.08

Eggs Crack 0.283/15.0 0.602/17.0 0.014/16.0 0.300/30.3 0.637(0.020) 0.29

Eggs Abnormal 0.504/15.0 1.348/17.0 0.055/16.0 0.636/29.3 1.095(0.046) 0.52

Eggs Set From Weeks 7 and 8 0.391/15.0 0.158/16.0 0.033/16.0 0.194/28.7 0.564(0.117) 0.24

Eggs Set From Weeks 6 7 and 8 0.916/15.0 0.283/16.0 0.094/16.0 0.431/27.2 1.688(0.105) 0.68

Eggs Fertile From Weeks 7 and 8 0.402/15.0 0.189/16.0 0.092/16.0 0.228/34.5 0.968(0.056) 0.39

Eggs Fertile From Weeks 6 7 and 8 0.999/15.0 0.325/16.0 0.173/16.0 0.499/29.9 3.077(0.028) 1.18

Eggs Viable From Weeks 7 and 8 0.385/15.0 0.183/16.0 0.101/16.0 0.223/35.5 1.676(0.007) 0.63

Eggs Viable From Weeks 6 7 and 8 0.958/15.0 0.308/16.0 0.213/16.0 0.493/31.3 6.223(<0.001) 2.23

Viable Eggs From Weeks 7 and 8 after Two Weeks of 

Incubation 0.385/15.0 0.170/16.0 0.111/16.0 0.222/35.6 1.484(0.013) 0.56

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of 

Incubation 0.958/15.0 0.302/16.0 0.229/16.0 0.496/31.6 5.773(<0.001) 2.08

Eggs Hatched 0.446/15.0 0.220/16.0 0.217/16.0 0.294/40.5 2.883(<0.001) 1.05

Number of 14-Day-Old Surviving Chicks 0.439/15.0 0.160/16.0 0.697/16.0 0.432/37.5 0.833(0.084) 0.40

Eggs Laid Per Hen Per Day 0.001/15.0 0.001/17.0 0.000/16.0 0.001/42.0 0.006(0.003) 0.00

Cracked Eggs as A Percentage of Eggs Laid 0.000/15.0 0.000/17.0 0.000/16.0 0.000/30.4 0.000(0.016) 0.00

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 
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Table 3. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F0 Generation

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (pvalues)c

Va

Av

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Reproduction (Continued) 

Abnormal Eggs as A Percentage of Eggs Laid 0.000/15.0 0.001/17.0 0.000/16.0 0.000/32.6 0.001(0.062) 0

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.000/15.0 0.000/16.0 0.000/16.0 0.000/39.7 0.000(0.785) 0

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.000/15.0 0.000/16.0 0.000/16.0 0.000/30.7 0.001(0.259) 0

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.001/15.0 0.000/16.0 0.000/16.0 0.001/32.9 0.002(0.100) 0

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.001/15.0 0.000/16.0 0.000/16.0 0.001/25.7 0.004(0.010) 0

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as 

A Percentage of Viable Eggs From Weeks 7 and 8 0.000/15.0 0.000/16.0 0.000/16.0 0.000/38.1 0.000(0.576) 0

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation 

as A Percentage of Viable Eggs From Weeks 6 7 and 8 0.000/15.0 0.000/16.0 0.000/16.0 0.000/40.5 0.000(0.613) 0

Eggs Hatched as A Percentage of Number of Viable Eggs from 

Weeks 7 and 8 after Two Weeks of Incubation 0.001/15.0 0.000/16.0 0.001/16.0 0.001/37.6 0.003(0.104) 0

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs 

Hatched 0.000/15.0 0.000/16.0 0.004/16.0 0.001/18.5 0.010(<0.001) 0

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 

7 and 8 0.002/15.0 0.000/16.0 0.001/16.0 0.001/37.3 0.007(0.007) 0

Eggs Hatched as A Percentage of Number of Fertile Eggs from 

Weeks 7 and 8 0.002/15.0 0.000/16.0 0.001/16.0 0.001/37.9 0.005(0.025) 0

Number of 14-Day-Old Surviving Chicks as A Percentage of 

Number of Eggs Set from Weeks 7 and 8 0.002/15.0 0.000/16.0 0.005/16.0 0.002/29.8 0.002(0.243) 0

Eggs Hatched Per Hen Per Day 0.002/15.0 0.001/16.0 0.001/16.0 0.002/40.7 0.015(<0.001) 0

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.002/15.0 0.001/16.0 0.004/16.0 0.002/37.6 0.004(0.093) 0

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 
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Table 3. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F0 Generation

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Varian

Averag

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.000/17.0 0.000/16.0 0.000/16.0 0.000/43.0 0.000(<0.001) 0.000/

Pen Mean Egg Content Weight - Albumin (g) 0.037/16.0 0.032/16.0 0.012/16.0 0.027/41.7 0.837(<0.001) 0.288/

Pen Mean Egg Content Weight - Yolk (g) 0.019/16.0 0.029/16.0 0.004/16.0 0.017/35.1 0.148(<0.001) 0.055/

Pen Mean Egg Content Weight - Combined (g) 0.082/16.0 0.100/16.0 0.022/16.0 0.068/38.7 0.704(<0.001) 0.257/

Thyroid Weight 

Adult Left Thyroid Weight (mg) - Males 0.689/15.0 1.979/13.0 0.166/16.0 0.945/24.0 12.499(<0.001) 4.467/

Adult Left Thyroid Weight (mg) - Females 0.804/15.0 1.323/12.0 0.287/14.0 0.805/29.9 6.996(<0.001) 2.592/

Bio Chem 

Egg Yolk Estradiol (pg/g) 8.606/4.0 17.119/4.0 43.712/4.0 23.145/8.5 630.494(<0.001) 217.76

Egg Yolk Testosterone (pg/g) 109854.4/4.0 488567.5/4.0 154690.0/4.0 251037.3/8.3 0.000(1.000) 56772.0

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.006/4.0 0.029/4.0 0.009/3.0 0.015/8.0 0.111(<0.001) 0.042/

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.008/4.0 0.003/4.0 0.009/4.0 0.007/10.7 0.030(0.008) 0.012/

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 1.525/4.0 0.456/4.0 0.132/4.0 0.704/7.0 0.000(1.000) 0.096/

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 0.301/4.0 1.137/4.0 0.056/4.0 0.498/6.4 0.489(0.270) 0.269/

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 0.282/4.0 0.366/4.0 2.254/4.0 0.967/6.4 0.335(0.554) 0.291/

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 2.962/4.0 14.337/4.0 3.327/4.0 6.875/7.6 44.047(<0.001) 16.804

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 100961.6/4.0 34117.10/4.0 268690.6/4.0 134589.8/7.8 744365.0(0.034) 289465

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 3985.248/4.0 34536.09/4.0 86131.77/4.0 41551.04/7.2 0.000(1.000) 3430.64

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 
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Table 4. Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Intervals within Lab

Laboratories, F0 Generationf

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Body Weight 

Adult Body Weight (g) Day 0 - Males 168.849(28.009) 11.205/38.5 2342.263/2.0 (50.675,287.023

Adult Body Weight (g) Day 56 - Males 184.637(31.530) 10.868/36.2 2971.612/2.0 (51.007,318.267

Adult Body Weight (g) Day 0 - Females 207.898(32.810) 28.448/38.8 3201.087/2.0 (71.621,344.176

Adult Body Weight (g) Day 56 - Females 227.716(36.232) 26.896/36.1 3911.403/2.0 (76.083,379.350

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 26.402(3.941) 0.460/49.8 46.140/2.0 (10.097,42.708) Adult Feed Consumption (g/bird/day) - Week 2 26.199(2.794) 0.868/41.5 22.560/1.9 (15.575,36.823) Adult Feed Consumption (g/bird/day) - Week 3 25.203(3.057) 0.614/38.5 27.430/1.9 (13.066,37.340) Adult Feed Consumption (g/bird/day) - Week 4 26.791(2.883) 0.827/28.1 24.132/1.9 (15.719,37.864) Adult Feed Consumption (g/bird/day) - Week 5 29.183(3.249) 0.743/28.8 30.931/1.9 (16.349,42.016) Adult Feed Consumption (g/bird/day) - Week 6 28.979(3.987) 0.624/32.3 47.072/1.9 (12.676,45.282) Adult Feed Consumption (g/bird/day) - Week 7 28.524(2.862) 0.483/45.7 24.088/1.9 (17.080,39.969) Adult Feed Consumption (g/bird/day) - Week 8 26.849(3.215) 0.309/29.9 20.360/1.0 (0.000,63.096) Adult Feed Consumption (g/bird/day) - Week 9 NA NA NA NA 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 

f. “NA” indicates that averages across laboratories were not calculated because data were available for only one laboratory. 
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Table 4. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

and Between Laboratories, F0 Generation

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Reproduction 

Eggs Laid 42.897(1.756) 3.095/32.2 6.643/1.0 (37.182,48.612) 

Eggs Crack 0.907(0.540) 0.300/30.3 0.637/1.0 (0.000,2.667) 

Eggs Abnormal 1.269(0.725) 0.636/29.3 1.095/0.9 (0.000,3.669) 

Eggs Set From Weeks 7 and 8 11.678(0.493) 0.194/28.7 0.564/1.2 (10.089,13.267) 

Eggs Set From Weeks 6 7 and 8 17.440(0.830) 0.431/27.2 1.688/1.3 (14.771,20.110) 

Eggs Fertile From Weeks 7 and 8 11.249(0.628) 0.228/34.5 0.968/1.3 (9.217,13.280) 

Eggs Fertile From Weeks 6 7 and 8 16.867(1.087) 0.499/29.9 3.077/1.5 (13.288,20.446) 

Eggs Viable From Weeks 7 and 8 10.915(0.794) 0.223/35.5 1.676/1.6 (8.252,13.578) 

Eggs Viable From Weeks 6 7 and 8 16.202(1.494) 0.493/31.3 6.223/1.7 (10.928,21.476) 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 10.841(0.752) 0.222/35.6 1.484/1.5 (8.343,13.340) 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 16.106(1.444) 0.496/31.6 5.773/1.7 (11.056,21.157) 

Eggs Hatched 9.907(1.029) 0.294/40.5 2.883/1.6 (6.367,13.447) 

Number of 14-Day-Old Surviving Chicks 8.790(0.639) 0.432/37.5 0.833/0.9 (6.689,10.892) 

Eggs Laid Per Hen Per Day 0.902(0.048) 0.001/42.0 0.006/1.6 (0.741,1.064) 

Cracked Eggs as A Percentage of Eggs Laid 0.022(0.013) 0.000/30.4 0.000/1.1 (0.000,0.065) 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 
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Table 4. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

and Between Laboratories, F0 Generation 

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Reproduction (Continued)

Abnormal Eggs as A Percentage of Eggs Laid 0.032(0.019) 0.000/32.6 0.001/0.9 (0.000,0.095) 

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.969(0.011) 0.000/39.7 0.000/0.1 (0.924,1.014) 

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.970(0.015) 0.000/30.7 0.001/1.0 (0.920,1.021) 

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.944(0.026) 0.001/32.9 0.002/1.2 (0.860,1.027) 

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.935(0.039) 0.001/25.7 0.004/1.6 (0.806,1.064) 

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 7 and 8 0.995(0.004) 0.000/38.1 0.000/0.3 (0.980,1.011) 

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 6 7 and 8 0.996(0.003) 0.000/40.5 0.000/0.3 (0.983,1.008) 

Eggs Hatched as A Percentage of Number of Viable Eggs from Weeks 7 and 8 

after Two Weeks of Incubation 0.910(0.033) 0.001/37.6 0.003/1.2 (0.803,1.018) 

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs Hatched 0.899(0.060) 0.001/18.5 0.010/1.6 (0.701,1.096) 

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 and 8 0.852(0.053) 0.001/37.3 0.007/1.5 (0.677,1.026) 

Eggs Hatched as A Percentage of Number of Fertile Eggs from Weeks 7 and 8 0.881(0.044) 0.001/37.9 0.005/1.4 (0.738,1.024) 

Number of 14-Day-Old Surviving Chicks as A Percentage of Number of Eggs 

Set from Weeks 7 and 8 0.768(0.035) 0.002/29.8 0.002/0.6 (0.644,0.892) 

Eggs Hatched Per Hen Per Day 0.708(0.074) 0.002/40.7 0.015/1.6 (0.455,0.961) 

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.627(0.045) 0.002/37.6 0.004/0.9 (0.478,0.776) 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 
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Table 4. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

and Between Laboratories, F0 Generation

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.215(0.009) 0.000/43.0 0.000/1.8 (0.168,0.262) 

Pen Mean Egg Content Weight - Albumin (g) 4.743(0.537) 0.027/41.7 0.837/1.9 (2.671,6.815) 

Pen Mean Egg Content Weight - Yolk (g) 3.640(0.234) 0.017/35.1 0.148/1.6 (2.845,4.434) 

Pen Mean Egg Content Weight - Combined (g) 8.394(0.507) 0.068/38.7 0.704/1.7 (6.642,10.147) 

Thyroid Weight 

Adult Left Thyroid Weight (mg) - Males 7.348(2.114) 0.945/24.0 12.499/1.7 (0.000,14.842) 

Adult Left Thyroid Weight (mg) - Females 9.096(1.610) 0.805/29.9 6.996/1.6 (3.621,14.571) 

Bio Chem 

Egg Yolk Estradiol (pg/g) 44.421(14.757) 23.145/8.5 630.494/1.9 (0.000,100.852) 

Egg Yolk Testosterone (pg/g) 2050.636(238.269) 251037.3/8.3 0.000/1.0 (839.691,3261.58

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.871(0.204) 0.015/8.0 0.111/1.6 (0.180,1.563) 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.860(0.110) 0.007/10.7 0.030/1.3 (0.496,1.223) 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 3.164(0.310) 0.704/7.0 0.000/1.0 (1.136,5.193) 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 1.763(0.519) 0.498/6.4 0.489/0.7 (0.000,3.753) 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 4.611(0.540) 0.967/6.4 0.335/0.3 (2.078,7.144) 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 18.452(4.099) 6.875/7.6 44.047/1.5 (4.722,32.182) 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 1694.232(538.020) 134589.8/7.8 744365.0/1.5 (0.000,3482.223

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 337.621(58.572) 41551.04/7.2 0.000/1.0 (0.000,822.809) 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 
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Table 5. Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Random Laboratory 

Generationd

Parameter 

Individual Laboratory Mean (Standard Error)a Average A

Lab1_ST Lab2_SV Lab3_WLI 

Mean 

(Standard Error) b 

Body Weight 

Adult Body Weight (g) Day 0 - Males 165.311 (1.914) 155.483 (3.072) 94.063 (2.950) 138.313 (22.289) 

Adult Body Weight (g) Day 56 - Males 213.494 (4.988) 216.300 (3.746) 119.133 (3.027) 182.906 (31.969) 

Adult Body Weight (g) Day 0 - Females 184.256 (4.289) 168.706 (3.977) 96.875 (3.657) 149.912 (26.924) 

Adult Body Weight (g) Day 56 - Females 267.650 (6.162) 266.482 (7.608) 153.313 (2.650) 228.910 (38.039) 

Mean Offspring Body Weight by Pen (g) Day 0 8.756 (0.191) 9.571 (0.173) 6.776 (0.147) 8.365 (0.831) 

Mean Offspring Body Weight by Pen (g) Day 14 82.688 (0.869) 78.929 (1.611) 48.529 (0.908) 70.035 (10.826) 

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 31.278 (0.587) 30.228 (0.551) 20.250 (0.704) 27.262 (3.508) Adult Feed Consumption (g/bird/day) - Week 2 31.444 (0.622) 36.561 (0.873) 21.250 (0.413) 29.728 (4.503) Adult Feed Consumption (g/bird/day) - Week 3 32.889 (0.911) 31.500 (1.156) 21.750 (0.413) 28.659 (3.535) Adult Feed Consumption (g/bird/day) - Week 4 32.333 (0.904) 25.844 (0.949) 22.500 (0.387) 26.854 (2.894) Adult Feed Consumption (g/bird/day) - Week 5 30.222 (0.794) 26.678 (1.010) 22.438 (0.695) 26.431 (2.273) Adult Feed Consumption (g/bird/day) - Week 6 32.611 (1.039) 27.444 (0.902) 22.467 (0.506) 27.453 (2.934) Adult Feed Consumption (g/bird/day) - Week 7 32.667 (0.878) 30.022 (0.685) 22.600 (0.388) 28.391 (3.023) Adult Feed Consumption (g/bird/day) - Week 8 32.556 (1.500) 29.117 (0.549) 21.600 (0.505) 27.657 (3.229) Adult Feed Consumption (g/bird/day) - Week 9 31.944 (1.259) 31.267 (0.622) NA 31.400 (0.557) 

Adult Feed Consumption (g/bird/day) - Week 10 NA 30.978 (0.536) NA NA Adult Feed Consumption (g/bird/day) - Week 11 NA 26.756 (1.416) NA NA 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 

d. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were av
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Table 5. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F1 Generation 

Parameter 

Individual Laboratory Mean (Standard Error)a Average 

Lab1_ST Lab2_SV Lab3_WLI 

Mean 

(Standard Error) b 

Reproduction 

Eggs Laid 46.222 (1.086) 39.444 (2.211) 43.688 (1.036) 43.527 (1.786) 

Eggs Crack 0.889 (0.342) 1.833 (0.480) 0.563 (0.302) 1.014 (0.346) 

Eggs Abnormal 0.722 (0.226) 3.389 (0.879) 0.375 (0.155) 1.304 (0.845) 

Eggs Set From Weeks 7 and 8 10.944 (0.563) 10.611 (0.696) 12.438 (0.316) 11.447 (0.599) 

Eggs Set From Weeks 6 7 and 8 16.722 (0.646) 16.167 (1.058) 39.188 (1.188) 24.007 (7.573) 

Eggs Fertile From Weeks 7 and 8 10.278 (0.722) 10.389 (0.701) 12.063 (0.622) 10.954 (0.598) 

Eggs Fertile From Weeks 6 7 and 8 15.667 (0.836) 15.611 (1.094) 37.875 (1.408) 23.020 (7.396) 

Eggs Viable From Weeks 7 and 8 10.222 (0.721) 10.167 (0.742) 17.250 (0.854) 12.528 (2.343) 

Eggs Viable From Weeks 6 7 and 8 15.333 (0.856) 15.278 (1.157) 36.375 (1.502) 22.290 (7.003) 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 10.222 (0.721) 10.056 (0.747) 16.938 (0.910) 12.377 (2.252) 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 15.333 (0.856) 15.167 (1.153) 16.938 (0.910) 15.873 (0.585) 

Eggs Hatched 9.333 (0.780) 9.389 (0.837) 11.438 (0.707) 10.100 (0.716) 

Number of 14-Day-Old Surviving Chicks 8.944 (0.769) 9.111 (0.808) 10.875 (0.724) 9.676 (0.635) 

Eggs Laid Per Hen Per Day 0.943 (0.022) 0.807 (0.045) 0.892 (0.021) 0.889 (0.036) 

Cracked Eggs as A Percentage of Eggs Laid 0.020 (0.008) 0.049 (0.014) 0.014 (0.008) 0.025 (0.009) 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 
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Table 5. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F1 Generation 

Parameter 

Individual Laboratory Mean (Standard Error)a Averag

Lab1_ST Lab2_SV Lab3_WLI 

Mean 

(Standard Erro

Reproduction (Continued) 

Abnormal Eggs as A Percentage of Eggs Laid 0.017 (0.005) 0.092 (0.026) 0.009 (0.004) 0.033 (0.023

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.925 (0.040) 0.979 (0.009) 0.956 (0.039) 0.970 (0.014

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.932 (0.028) 0.965 (0.017) 0.963 (0.017) 0.959 (0.011

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.920 (0.039) 0.956 (0.021) 0.935 (0.033) 0.945 (0.016

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.913 (0.031) 0.943 (0.029) 0.924 (0.021) 0.927 (0.015

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as A Percentage 

of Viable Eggs From Weeks 7 and 8 1.000 (0.000) 0.989 (0.008) 0.981 (0.016) 0.995 (0.005

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 6 7 and 8 1.000 (0.000) 0.993 (0.005) 0.981 (0.016) 0.997 (0.003

Eggs Hatched as A Percentage of Number of Viable Eggs from Weeks 7 and 8 

after Two Weeks of Incubation 0.905 (0.033) 0.917 (0.040) 0.983 (0.009) 0.944 (0.028

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs Hatched 0.956 (0.019) 0.976 (0.012) 0.949 (0.017) 0.964 (0.009

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 and 8 0.830 (0.047) 0.869 (0.045) 0.904 (0.050) 0.866 (0.027

Eggs Hatched as A Percentage of Number of Fertile Eggs from Weeks 7 and 8 0.901 (0.034) 0.888 (0.045) 0.936 (0.030) 0.913 (0.020

Number of 14-Day-Old Surviving Chicks as A Percentage of Number of Eggs Set 

from Weeks 7 and 8 0.796 (0.049) 0.846 (0.044) 0.860 (0.051) 0.834 (0.028

Eggs Hatched Per Hen Per Day 0.667 (0.056) 0.671 (0.060) 0.817 (0.051) 0.722 (0.051

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.639 (0.055) 0.651 (0.058) 0.777 (0.052) 0.691 (0.045

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 
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Table 5. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F1 Generation 

Parameter 

Individual Laboratory Mean (Standard Error) a Average 

Lab1_ST Lab2_SV Lab3_WLI Mean (Standard Error) b 

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.204 (0.003) 0.207 (0.004) 0.229 (0.003) 0.213 (0.008) 

Pen Mean Egg Content Weight - Albumin (g) 6.475 (0.131) 4.456 (0.208) 4.825 (0.103) 5.257 (0.621) 

Pen Mean Egg Content Weight - Yolk (g) 3.821 (0.085) 3.784 (0.106) 3.275 (0.091) 3.626 (0.177) 

Pen Mean Egg Content Weight - Combined (g) 10.295 (0.193) 8.239 (0.297) 8.100 (0.133) 8.881 (0.713) 

First Foam First Egg Cloacal Size 

Age in Days at First Foam - Males 34.500 (0.283) 42.611 (3.422) 37.688 (0.810) 37.227 (1.912) 

Age in Days at First Egg - Females 42.722 (0.666) 48.278 (1.885) 45.063 (1.047) 44.964 (1.506) 

Cloacal Area (cm2) at 4 Weeks - Males 0.292 (0.020) 0.222 (0.018) 0.253 (0.021) 0.255 (0.021) Cloacal Area (cm2) at 5 Weeks - Males 0.372 (0.030) 0.279 (0.029) 0.539 (0.073) 0.384 (0.070) Cloacal Area (cm2) at 6 Weeks - Males 0.558 (0.029) 0.423 (0.029) 0.664 (0.041) 0.546 (0.069) Cloacal Area (cm2) at 7 Weeks - Males NA NA 0.593 (0.035) NA Cloacal Area (cm2) at 8 Weeks - Males NA NA 0.582 (0.025) NA Cloacal Area (cm2) at 9 Weeks - Males NA NA 0.589 (0.023) NA 

Cloacal Area (cm2) at 4 Weeks - Females 0.274 (0.019) 0.213 (0.029) 0.244 (0.021) 0.249 (0.017) Cloacal Area (cm2) at 5 Weeks - Females 0.402 (0.051) 0.111 (0.014) 0.524 (0.086) 0.336 (0.124) Cloacal Area (cm2) at 6 Weeks - Females 0.521 (0.040) 0.103 (0.010) 0.956 (0.120) 0.515 (0.244) Cloacal Area (cm2) at 7 Weeks - Females NA NA 0.652 (0.044) NA Cloacal Area (cm2) at 8 Weeks - Females NA NA 0.638 (0.035) NA Cloacal Area (cm2) at 9 Weeks - Females NA NA 0.679 (0.033) NA 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 

d. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were av
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Table 5. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F1 Generation 

Parameter 

Individual Laboratory Mean (Standard Error) a Average 

Lab1_ST Lab2_SV Lab3_WLI Mean (Standard Error) b 

Mating Behavior 

Length of Time to First Neck Grab (sec) - Day 1 21.286 (11.720) 15.143 (6.780) 7.571 (1.571) 10.208 (3.500) Length of Time to First Neck Grab (sec) - Day 2 6.571 (2.707) 8.286 (1.848) 5.286 (1.304) 6.393 (1.087) Length of Time to First Neck Grab (sec) - Day 3 3.000 (0.309) 4.286 (1.063) 3.143 (0.340) 3.118 (0.223) 

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 -18.286 (11.478) -10.857 (5.881) -4.429 (1.412) -6.676 (3.072) 

Length of Time to First Mount (sec) - Day 1 61.571 (40.199) 39.571 (16.959) 57.143 (40.865) 44.713 (14.595) Length of Time to First Mount (sec) - Day 2 13.143 (5.157) 15.000 (5.196) 51.286 (41.543) 14.351 (3.646) Length of Time to First Mount (sec) - Day 3 5.714 (1.392) 15.714 (11.401) 5.000 (0.900) 5.256 (0.754) 

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 -55.857 (40.219) -23.857 (14.175) -52.143 (40.716) -29.801 (12.702) 

Number of Mount Attempts (count) - Day 1 7.857 (1.262) 5.286 (1.040) 5.429 (1.088) 6.055 (0.761) Number of Mount Attempts (count) - Day 2 8.286 (2.212) 12.429 (3.351) 3.714 (1.085) 7.405 (2.483) Number of Mount Attempts (count) - Day 3 6.000 (1.195) 13.429 (3.123) 5.286 (1.554) 7.425 (2.083) 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 -1.857 (1.945) 8.143 (2.272) -0.143 (1.388) 1.896 (2.982) 

Number of Cloacal Contacts (count) - Day 1 1.714 (0.565) 2.857 (0.595) 2.714 (1.017) 2.339 (0.439) Number of Cloacal Contacts (count) - Day 2 2.143 (0.769) 5.429 (1.631) 3.143 (0.986) 3.075 (0.760) Number of Cloacal Contacts (count) - Day 3 2.857 (0.769) 7.571 (1.462) 3.000 (0.488) 4.203 (1.391) 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 1.143 (0.508) 4.714 (1.459) 0.286 (1.085) 1.811 (1.178) 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 6.429 (0.922) 7.714 (1.340) 0.857 (0.261) 4.859 (2.149) Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 3.286 (0.474) 5.000 (1.327) 1.000 (0.436) 2.863 (1.108) Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 2.429 (0.429) 7.571 (1.212) 0.714 (0.286) 3.425 (1.994) 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 

to Day 3 -4.000 (1.024) -0.143 (1.779) -0.143 (0.340) -1.460 (1.331) 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 
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Table 5. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F1 Generation 

Parameter 

Individual Laboratory Mean (Standard Error) a Average 

Lab1_ST Lab2_SV Lab3_WLI Mean (Standard Error) b 

Thyroid Weight 

15 Day Embryo (ED15) Thyroid Weight (mg) 0.991 (0.143) 2.048 (0.146) 2.371 (0.208) 1.795 (0.417) 

Adult Left Thyroid Weight (mg) - Males 10.022 (1.061) 6.807 (0.681) 5.533 (0.679) 7.345 (1.284) 

Adult Left Thyroid Weight (mg) - Females 13.322 (1.203) 15.460 (1.893) 6.275 (0.743) 11.506 (2.812) 

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) 48.413 (6.569) 54.965 (4.077) 52.979 (4.702) 53.085 (2.789) 

Bio Chem 

Egg Yolk Estradiol (pg/g) 92.541 (13.371) 43.908 (13.558) 66.995 (10.430) 67.845 (13.405) 

Egg Yolk Testosterone (pg/g) 5170.843 (672.003) 1971.184 (257.929) 2221.775 (372.648) 3041.891 (987.311) 

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.718 (0.075) 0.620 (0.044) 0.992 (0.129) 0.748 (0.099) 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.761 (0.098) 0.755 (0.064) 0.927 (0.076) 0.815 (0.059) 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 6.385 (1.309) 1.829 (0.517) 3.574 (1.131) 3.722 (1.322) 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 2.843 (0.730) 2.590 (0.779) 1.930 (0.365) 2.195 (0.310) 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 5.385 (0.764) 10.140 (4.754) 6.391 (2.691) 5.569 (0.727) 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 22.013 (4.460) 14.819 (1.889) 17.685 (3.163) 16.668 (1.810) 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 3237.805 (273.449) 478.438 (127.383) 1596.371 (549.294) 1759.598 (688.531) 

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 531.493 (198.570) 392.223 (57.767) 276.222 (138.471) 385.548 (51.490) 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 
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Table 6. Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component Analysis with 

F1 Generationf

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Variance 

Average/

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Body Weight 

Adult Body Weight (g) Day 0 - Males 3.663/17.0 9.438/17.0 8.704/15.0 7.268/42.9 1483.097(<0.001) 496.787/2

Adult Body Weight (g) Day 56 - Males 24.879/17.0 14.033/17.0 9.161/14.0 16.024/42.8 3050.007(<0.001) 1022.006/

Adult Body Weight (g) Day 0 - Females 18.394/17.0 15.813/17.0 13.374/15.0 15.860/48.7 2158.778(<0.001) 724.879/2

Adult Body Weight (g) Day 56 - Females 37.968/17.0 57.884/16.0 7.023/15.0 34.292/35.6 4306.620(<0.001) 1446.937/

Mean Offspring Body Weight by Pen (g) Day 0 0.036/15.0 0.030/16.0 0.022/16.0 0.029/44.5 2.044(<0.001) 0.691/2.

Mean Offspring Body Weight by Pen (g) Day 14 0.755/15.0 2.595/16.0 0.824/16.0 1.392/34.8 350.216(<0.001) 117.202/2

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 0.345/17.0 0.304/17.0 0.496/15.0 0.382/45.5 36.545(<0.001) 12.309/2Adult Feed Consumption (g/bird/day) - Week 2 0.387/17.0 0.762/17.0 0.171/15.0 0.440/38.8 60.393(<0.001) 20.277/2Adult Feed Consumption (g/bird/day) - Week 3 0.829/17.0 1.337/17.0 0.171/15.0 0.779/37.0 36.719(<0.001) 12.497/2Adult Feed Consumption (g/bird/day) - Week 4 0.817/17.0 0.901/17.0 0.150/15.0 0.623/39.4 24.500(<0.001) 8.373/2.Adult Feed Consumption (g/bird/day) - Week 5 0.631/17.0 1.020/17.0 0.483/15.0 0.711/45.5 14.792(<0.001) 5.167/2.Adult Feed Consumption (g/bird/day) - Week 6 1.079/17.0 0.814/17.0 0.256/14.0 0.716/41.2 25.105(<0.001) 8.606/2.Adult Feed Consumption (g/bird/day) - Week 7 0.771/17.0 0.469/17.0 0.150/14.0 0.464/39.0 26.956(<0.001) 9.139/2.Adult Feed Consumption (g/bird/day) - Week 8 2.250/17.0 0.302/17.0 0.255/14.0 0.936/25.6 30.378(<0.001) 10.429/2Adult Feed Consumption (g/bird/day) - Week 9 1.585/17.0 0.386/17.0 NA 0.986/24.8 0.000(1.000) 0.311/24

Adult Feed Consumption (g/bird/day) - Week 10 NA 0.288/17.0 NA NA NA NA Adult Feed Consumption (g/bird/day) - Week 11 NA 2.006/17.0 NA NA NA NA 

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 

f. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were ava
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Table 6. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F1 Generation 

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Variance 

Average/

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Reproduction 

Eggs Laid 1.180/17.0 4.890/17.0 1.073/15.0 2.381/32.6 7.477(0.085) 3.191/2.

Eggs Crack 0.117/17.0 0.230/17.0 0.091/15.0 0.146/42.9 0.221(0.337) 0.119/2.

Eggs Abnormal 0.051/17.0 0.772/17.0 0.024/15.0 0.282/20.4 1.907(0.032) 0.713/2.

Eggs Set From Weeks 7 and 8 0.317/17.0 0.485/17.0 0.100/15.0 0.300/39.8 0.801(0.032) 0.359/2.

Eggs Set From Weeks 6 7 and 8 0.417/17.0 1.119/17.0 1.410/15.0 0.982/40.1 171.052(<0.001) 57.344/2

Eggs Fertile From Weeks 7 and 8 0.522/17.0 0.491/17.0 0.387/15.0 0.467/48.8 0.608(0.301) 0.357/2.

Eggs Fertile From Weeks 6 7 and 8 0.699/17.0 1.197/17.0 1.982/15.0 1.293/40.1 162.811(<0.001) 54.701/2

Eggs Viable From Weeks 7 and 8 0.520/17.0 0.551/17.0 0.729/15.0 0.600/46.8 15.863(<0.001) 5.487/2.

Eggs Viable From Weeks 6 7 and 8 0.732/17.0 1.339/17.0 2.257/15.0 1.443/39.3 145.682(<0.001) 49.041/2

Viable Eggs From Weeks 7 and 8 after Two Weeks of 

Incubation 0.520/17.0 0.559/17.0 0.829/15.0 0.636/45.4 14.582(<0.001) 5.072/2.

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of 

Incubation 0.732/17.0 1.328/17.0 0.829/15.0 0.963/46.1 0.118(0.896) 0.342/2.

Eggs Hatched 0.608/17.0 0.700/17.0 0.500/15.0 0.603/48.6 0.938(0.234) 0.512/2.

Number of 14-Day-Old Surviving Chicks 0.591/17.0 0.653/17.0 0.524/15.0 0.589/48.9 0.623(0.389) 0.403/2.

Eggs Laid Per Hen Per Day 0.000/17.0 0.002/17.0 0.000/15.0 0.001/32.6 0.003(0.092) 0.001/2.

Cracked Eggs as A Percentage of Eggs Laid 0.000/17.0 0.000/17.0 0.000/15.0 0.000/37.6 0.000(0.399) 0.000/1.

Abnormal Eggs as A Percentage of Eggs Laid 0.000/17.0 0.001/17.0 0.000/15.0 0.000/19.1 0.001(0.080) 0.001/2.

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 
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Table 6. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F1 Generation 

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Variance 

Average/

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Reproduction (Continued) 

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 

and 8 0.002/17.0 0.000/17.0 0.002/15.0 0.001/33.7 0.000(0.805) 0.000/3.

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 

and 8 0.001/17.0 0.000/17.0 0.000/15.0 0.000/39.5 0.000(1.000) 0.000/3.

Viable Eggs as A Percentage of Eggs Set From Weeks 7 

and 8 0.002/17.0 0.000/17.0 0.001/15.0 0.001/40.9 0.000(1.000) 0.000/13

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 

and 8 0.001/17.0 0.001/17.0 0.000/15.0 0.001/46.2 0.000(1.000) 0.000/9.

Viable Eggs from Weeks 7 and 8 after Two Weeks of 

Incubation as A Percentage of Viable Eggs From Weeks 7 

and 8 

0.000/17.0 0.000/17.0 0.000/15.0 0.000/23.4 0.000(0.529) 0.000/1.

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of 

Incubation as A Percentage of Viable Eggs From Weeks 6 

7 and 8 

0.000/17.0 0.000/17.0 0.000/15.0 0.000/19.0 0.000(0.747) 0.000/1.

Eggs Hatched as A Percentage of Number of Viable Eggs 

from Weeks 7 and 8 after Two Weeks of Incubation 0.001/17.0 0.002/17.0 0.000/15.0 0.001/35.0 0.002(0.067) 0.001/2.

Number of 14-Day-Old Surviving Chicks as A Percentage 

of Eggs Hatched 0.000/17.0 0.000/17.0 0.000/15.0 0.000/43.4 0.000(0.875) 0.000/1.

Eggs Hatched as A Percentage of Number of Eggs Set 

from Weeks 7 and 8 0.002/17.0 0.002/17.0 0.002/15.0 0.002/47.9 0.000(1.000) 0.001/32

Eggs Hatched as A Percentage of Number of Fertile Eggs 

from Weeks 7 and 8 0.001/17.0 0.002/17.0 0.001/15.0 0.001/44.6 0.000(1.000) 0.000/20

Number of 14-Day-Old Surviving Chicks as A Percentage 

of Number of Eggs Set from Weeks 7 and 8 0.002/17.0 0.002/17.0 0.003/15.0 0.002/47.6 0.000(1.000) 0.001/33

Eggs Hatched Per Hen Per Day 0.003/17.0 0.004/17.0 0.003/15.0 0.003/48.6 0.005(0.235) 0.003/2.

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.003/17.0 0.003/17.0 0.003/15.0 0.003/48.9 0.003(0.387) 0.002/2.

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 
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31 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among laboratory 

for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory variance, Si2 and d

degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 
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Table 6. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F1 Generationf 

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Variance 

Average/

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.000/17.0 0.000/17.0 0.000/15.0 0.000/45.7 0.000(<0.001) 0.000/1.

Pen Mean Egg Content Weight - Albumin (g) 0.017/17.0 0.043/17.0 0.011/15.0 0.024/37.4 1.133(<0.001) 0.385/2.

Pen Mean Egg Content Weight - Yolk (g) 0.007/17.0 0.011/17.0 0.008/15.0 0.009/47.5 0.085(<0.001) 0.031/2.

Pen Mean Egg Content Weight - Combined (g) 0.037/17.0 0.088/17.0 0.018/15.0 0.048/36.7 1.478(<0.001) 0.508/2.

First Foam First Egg Cloacal Size

Age in Days at First Foam - Males 0.080/17.0 11.707/17.0 0.656/15.0 4.148/19.1 8.558(<0.001) 3.655/2.

Age in Days at First Egg - Females 0.443/17.0 3.554/17.0 1.096/15.0 1.698/31.1 5.337(0.036) 2.268/2.

Cloacal Area (cm2) at 4 Weeks - Males 0.000/17.0 0.000/17.0 0.000/15.0 0.000/47.8 0.001(0.113) 0.000/2.Cloacal Area (cm2) at 5 Weeks - Males 0.001/17.0 0.001/17.0 0.005/15.0 0.002/25.2 0.013(0.011) 0.005/2.Cloacal Area (cm2) at 6 Weeks - Males 0.001/17.0 0.001/17.0 0.002/15.0 0.001/41.7 0.013(<0.001) 0.005/2.Cloacal Area (cm2) at 7 Weeks - Males NA NA 0.001/15.0 NA NA NA Cloacal Area (cm2) at 8 Weeks - Males NA NA 0.001/15.0 NA NA NA Cloacal Area (cm2) at 9 Weeks - Males NA NA 0.001/15.0 NA NA NA 

Cloacal Area (cm2) at 4 Weeks - Females 0.000/17.0 0.001/17.0 0.000/15.0 0.001/43.5 0.000(0.633) 0.000/2.Cloacal Area (cm2) at 5 Weeks - Females 0.003/17.0 0.000/17.0 0.007/15.0 0.003/25.5 0.043(<0.001) 0.015/2.Cloacal Area (cm2) at 6 Weeks - Females 0.002/17.0 0.000/17.0 0.014/15.0 0.005/18.5 0.173(<0.001) 0.059/2.Cloacal Area (cm2) at 7 Weeks - Females NA NA 0.002/15.0 NA NA NA Cloacal Area (cm2) at 8 Weeks - Females NA NA 0.001/15.0 NA NA NA Cloacal Area (cm2) at 9 Weeks - Females NA NA 0.001/15.0 NA NA NA 

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 

f. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were ava
DRAFT 

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Table 6. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F1 Generation 

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (pvalues)c

Va

Av

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Mating Behavior 

Length of Time to First Neck Grab (sec) - Day 1 137.367/6.0 45.973/6.0 2.469/6.0 61.937/9.9 14.616(0.656) 12Length of Time to First Neck Grab (sec) - Day 2 7.327/6.0 3.415/6.0 1.701/6.0 4.147/13.6 0.471(0.870) 1Length of Time to First Neck Grab (sec) - Day 3 0.095/6.0 1.129/6.0 0.116/6.0 0.447/8.3 0.000(1.000) 0

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 131.748/6.0 34.592/6.0 1.993/6.0 56.111/9.2 10.991(0.677) 9

Length of Time to First Mount (sec) - Day 1 1615.946/6.0 287.612/6.0 1669.925/6.0 1191.161/14.0 0.000(1.000) 213Length of Time to First Mount (sec) - Day 2 26.592/6.0 27.000/6.0 1725.796/6.0 593.129/6.4 0.000(1.000) 13Length of Time to First Mount (sec) - Day 3 1.939/6.0 129.986/6.0 0.810/6.0 44.245/6.3 0.000(1.000) 0

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 1617.544/6.0 200.925/6.0 1657.782/6.0 1158.751/13.4 0.000(1.000) 161

Number of Mount Attempts (count) - Day 1 1.592/6.0 1.082/6.0 1.184/6.0 1.286/17.5 0.479(0.764) 0Number of Mount Attempts (count) - Day 2 4.891/6.0 11.231/6.0 1.177/6.0 5.766/11.9 13.582(0.051) 6Number of Mount Attempts (count) - Day 3 1.429/6.0 9.755/6.0 2.415/6.0 4.533/10.8 9.331(0.433) 4

Change in Number of Mount Attempts (count) from Day 1 to Day 3 3.782/6.0 5.163/6.0 1.925/6.0 3.624/15.9 23.118(0.010) 8

Number of Cloacal Contacts (count) - Day 1 0.320/6.0 0.354/6.0 1.034/6.0 0.569/13.5 0.125(0.761) 0Number of Cloacal Contacts (count) - Day 2 0.592/6.0 2.660/6.0 0.973/6.0 1.408/12.8 0.644(0.682) 0Number of Cloacal Contacts (count) - Day 3 0.592/6.0 2.136/6.0 0.238/6.0 0.989/10.6 4.921(0.081) 1

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 0.259/6.0 2.129/6.0 1.177/6.0 1.188/12.7 3.113(0.242) 1

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 0.850/6.0 1.796/6.0 0.068/6.0 0.905/11.2 12.987(<0.001) 4Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 0.224/6.0 1.762/6.0 0.190/6.0 0.726/8.9 3.081(<0.001) 1Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 0.184/6.0 1.469/6.0 0.082/6.0 0.578/8.2 11.380(<0.001) 3

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 

to Day 3 1.048/6.0 3.163/6.0 0.116/6.0 1.442/10.1 4.142(0.005) 1

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 
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Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

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d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 
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Table 6. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F1 Generation 

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Variance 

Average/

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Thyroid Weight 

15 Day Embryo (ED15) Thyroid Weight (mg) 0.020/10.0 0.021/16.0 0.043/16.0 0.028/38.6 0.494(<0.001) 0.174/2.

Adult Left Thyroid Weight (mg) - Males 1.126/17.0 0.463/13.0 0.461/14.0 0.684/39.6 4.285(0.011) 1.650/2.

Adult Left Thyroid Weight (mg) - Females 1.448/17.0 3.583/14.0 0.552/15.0 1.861/29.4 21.932(<0.001) 7.909/2.

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring 

(%) 43.147/15.0 16.622/16.0 22.105/16.0 27.291/39.0 0.000(1.000) 7.777/39

Bio Chem 

Egg Yolk Estradiol (pg/g) 178.782/4.0 183.809/4.0 108.779/4.0 157.123/11.5 384.257(0.163) 179.705/2

Egg Yolk Testosterone (pg/g) 451587.9/3.0 66527.31/4.0 138866.2/4.0 218993.8/5.8 2714558(<0.001) 974782.9/

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.006/4.0 0.002/3.0 0.017/4.0 0.008/7.5 0.023(0.102) 0.010/2.

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.010/4.0 0.004/3.0 0.006/4.0 0.006/10.3 0.004(0.521) 0.003/2.

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 1.714/4.0 0.267/4.0 1.280/4.0 1.087/9.2 4.230(0.014) 1.748/2.

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 0.533/4.0 0.607/4.0 0.133/4.0 0.424/9.7 0.011(0.971) 0.096/2.

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 0.584/4.0 22.605/4.0 7.244/4.0 10.144/6.6 0.000(1.000) 0.528/6.

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 19.888/4.0 3.569/4.0 10.003/4.0 11.153/8.8 2.081(0.820) 3.276/2.

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 74774.41/4.0 16226.49/4.0 301724.0/4.0 130908.3/6.4 1301403(<0.001) 474075.0/

Plasma Sex Hormone Testosterone - Adult Females 

(pg/mL) 39429.91/4.0 3336.991/4.0 19174.31/4.0 20647.07/7.9 0.000(1.000) 2651.214/

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 
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Table 7. Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Intervals within Lab

Laboratories, F1 Generationf

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Body Weight 

Adult Body Weight (g) Day 0 - Males 138.313(22.289) 7.268/42.9 1483.097/2.0 (44.316,232.311

Adult Body Weight (g) Day 56 - Males 182.906(31.969) 16.024/42.8 3050.007/2.0 (48.272,317.540

Adult Body Weight (g) Day 0 - Females 149.912(26.924) 15.860/48.7 2158.778/2.0 (37.435,262.389

Adult Body Weight (g) Day 56 - Females 228.910(38.039) 34.292/35.6 4306.620/2.0 (70.370,387.451

Mean Offspring Body Weight by Pen (g) Day 0 8.365(0.831) 0.029/44.5 2.044/1.9 (4.978,11.752) 

Mean Offspring Body Weight by Pen (g) Day 14 70.035(10.826) 1.392/34.8 350.216/2.0 (24.211,115.859

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 27.262(3.508) 0.382/45.5 36.545/2.0 (12.772,41.752) Adult Feed Consumption (g/bird/day) - Week 2 29.728(4.503) 0.440/38.8 60.393/2.0 (10.912,48.544) Adult Feed Consumption (g/bird/day) - Week 3 28.659(3.535) 0.779/37.0 36.719/1.9 (14.575,42.744) Adult Feed Consumption (g/bird/day) - Week 4 26.854(2.894) 0.623/39.4 24.500/1.9 (15.469,38.240) Adult Feed Consumption (g/bird/day) - Week 5 26.431(2.273) 0.711/45.5 14.792/1.8 (17.969,34.893) Adult Feed Consumption (g/bird/day) - Week 6 27.453(2.934) 0.716/41.2 25.105/1.9 (16.012,38.895) Adult Feed Consumption (g/bird/day) - Week 7 28.391(3.023) 0.464/39.0 26.956/1.9 (16.193,40.590) Adult Feed Consumption (g/bird/day) - Week 8 27.657(3.229) 0.936/25.6 30.378/1.9 (15.146,40.168) Adult Feed Consumption (g/bird/day) - Week 9 31.400(0.557) 0.986/24.8 0.000/1.0 (29.097,33.702) 

Adult Feed Consumption (g/bird/day) - Week 10 NA NA NA NA Adult Feed Consumption (g/bird/day) - Week 11 NA NA NA NA 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 

f. “NA” denotes that that averages across laboratories were not calculated because data were available for only one laboratory. 
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Table 7. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

 Laboratory and Between Laboratories, F1 Generation 

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Reproduction 

Eggs Laid 43.527(1.786) 2.381/32.6 7.477/1.2 (37.787,49.267) 

Eggs Crack 1.014(0.346) 0.146/42.9 0.221/0.8 (0.000,2.170) 

Eggs Abnormal 1.304(0.845) 0.282/20.4 1.907/1.6 (0.000,4.081) 

Eggs Set From Weeks 7 and 8 11.447(0.599) 0.300/39.8 0.801/1.1 (9.508,13.386) 

Eggs Set From Weeks 6 7 and 8 24.007(7.573) 0.982/40.1 171.052/2.0 (0.000,55.838) 

Eggs Fertile From Weeks 7 and 8 10.954(0.598) 0.467/48.8 0.608/0.6 (8.930,12.979) 

Eggs Fertile From Weeks 6 7 and 8 23.020(7.396) 1.293/40.1 162.811/2.0 (0.000,53.848) 

Eggs Viable From Weeks 7 and 8 12.528(2.343) 0.600/46.8 15.863/1.9 (3.605,21.451) 

Eggs Viable From Weeks 6 7 and 8 22.290(7.003) 1.443/39.3 145.682/2.0 (0.000,51.269) 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 12.377(2.252) 0.636/45.4 14.582/1.8 (3.911,20.842) 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 15.873(0.585) 0.963/46.1 0.118/0.0 (13.507,18.239) 

Eggs Hatched 10.100(0.716) 0.603/48.6 0.938/0.7 (7.710,12.490) 

Number of 14-Day-Old Surviving Chicks 9.676(0.635) 0.589/48.9 0.623/0.5 (7.485,11.868) 

Eggs Laid Per Hen Per Day 0.889(0.036) 0.001/32.6 0.003/1.2 (0.773,1.004) 

Cracked Eggs as A Percentage of Eggs Laid 0.025(0.009) 0.000/37.6 0.000/0.8 (0.000,0.057) 

Abnormal Eggs as A Percentage of Eggs Laid 0.033(0.023) 0.000/19.1 0.001/1.5 (0.000,0.107) 

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.970(0.014) 0.001/33.7 0.000/0.1 (0.898,1.042) 

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.959(0.011) 0.000/39.5 0.000/1.0 (0.911,1.007) 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 
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Table 7. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

 Laboratory and Between Laboratories, F1 Generation 

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Reproduction (Continued)

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.945(0.016) 0.001/40.9 0.000/1.0 (0.873,1.017) 

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.927(0.015) 0.001/46.2 0.000/1.0 (0.864,0.989) 

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 7 and 8 0.995(0.005) 0.000/23.4 0.000/0.3 (0.970,1.020) 

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 6 7 and 8 0.997(0.003) 0.000/19.0 0.000/0.1 (0.975,1.018) 

Eggs Hatched as A Percentage of Number of Viable Eggs from Weeks 7 and 8 

after Two Weeks of Incubation 0.944(0.028) 0.001/35.0 0.002/0.9 (0.852,1.037) 

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs Hatched 0.964(0.009) 0.000/43.4 0.000/0.0 (0.925,1.003) 

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 and 8 0.866(0.027) 0.002/47.9 0.000/1.0 (0.758,0.974) 

Eggs Hatched as A Percentage of Number of Fertile Eggs from Weeks 7 and 8 0.913(0.020) 0.001/44.6 0.000/1.0 (0.830,0.997) 

Number of 14-Day-Old Surviving Chicks as A Percentage of Number of Eggs 

Set from Weeks 7 and 8 0.834(0.028) 0.002/47.6 0.000/1.0 (0.723,0.945) 

Eggs Hatched Per Hen Per Day 0.722(0.051) 0.003/48.6 0.005/0.7 (0.551,0.893) 

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.691(0.045) 0.003/48.9 0.003/0.5 (0.534,0.848) 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 
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Table 7. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

and Between Laboratories, F1 Generation

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.213(0.008) 0.000/45.7 0.000/1.8 (0.149,0.277) 

Pen Mean Egg Content Weight - Albumin (g) 5.257(0.621) 0.024/37.4 1.133/1.9 (2.781,7.733) 

Pen Mean Egg Content Weight - Yolk (g) 3.626(0.177) 0.009/47.5 0.085/1.6 (3.019,4.233) 

Pen Mean Egg Content Weight - Combined (g) 8.881(0.713) 0.048/36.7 1.478/1.9 (6.128,11.635) 

First Foam First Egg Cloacal Size

Age in Days at First Foam - Males 37.227(1.912) 4.148/19.1 8.558/1.2 (31.006,43.448) 

Age in Days at First Egg - Females 44.964(1.506) 1.698/31.1 5.337/1.2 (40.129,49.800) 

Cloacal Area (cm2) at 4 Weeks - Males 0.255(0.021) 0.000/47.8 0.001/1.0 (0.187,0.323) Cloacal Area (cm2) at 5 Weeks - Males 0.384(0.070) 0.002/25.2 0.013/1.5 (0.155,0.613) Cloacal Area (cm2) at 6 Weeks - Males 0.546(0.069) 0.001/41.7 0.013/1.7 (0.302,0.790) Cloacal Area (cm2) at 7 Weeks - Males NA NA NA NA Cloacal Area (cm2) at 8 Weeks - Males NA NA NA NA Cloacal Area (cm2) at 9 Weeks - Males NA NA NA NA 

Cloacal Area (cm2) at 4 Weeks - Females 0.249(0.017) 0.001/43.5 0.000/0.3 (0.187,0.310) Cloacal Area (cm2) at 5 Weeks - Females 0.336(0.124) 0.003/25.5 0.043/1.7 (0.000,0.773) Cloacal Area (cm2) at 6 Weeks - Females 0.515(0.244) 0.005/18.5 0.173/1.9 (0.000,1.459) Cloacal Area (cm2) at 7 Weeks - Females NA NA NA NA Cloacal Area (cm2) at 8 Weeks - Females NA NA NA NA Cloacal Area (cm2) at 9 Weeks - Females NA NA NA NA 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 
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Table 7. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

and Between Laboratories, F1 Generation

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborato

Mating Behavior 

Length of Time to First Neck Grab (sec) - Day 1 10.208(3.500) 61.937/9.9 14.616/0.2 (0.000,28.847Length of Time to First Neck Grab (sec) - Day 2 6.393(1.087) 4.147/13.6 0.471/0.0 (1.490,11.296Length of Time to First Neck Grab (sec) - Day 3 3.118(0.223) 0.447/8.3 0.000/1.0 (1.551,4.686) 

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 -6.676(3.072) 56.111/9.2 10.991/0.2 (-24.354,11.003

Length of Time to First Mount (sec) - Day 1 44.713(14.595) 1191.161/14.0 0.000/1.0 (0.000,123.195Length of Time to First Mount (sec) - Day 2 14.351(3.646) 593.129/6.4 0.000/1.0 (0.000,73.188Length of Time to First Mount (sec) - Day 3 5.256(0.754) 44.245/6.3 0.000/1.0 (0.000,21.383

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 -29.801(12.702) 1158.751/13.4 0.000/1.0 (-106.430,46.82

Number of Mount Attempts (count) - Day 1 6.055(0.761) 1.286/17.5 0.479/0.2 (3.103,9.007) Number of Mount Attempts (count) - Day 2 7.405(2.483) 5.766/11.9 13.582/1.1 (0.000,15.613Number of Mount Attempts (count) - Day 3 7.425(2.083) 4.533/10.8 9.331/1.0 (0.470,14.380

Change in Number of Mount Attempts (count) from Day 1 to Day 3 1.896(2.982) 3.624/15.9 23.118/1.5 (-8.041,11.832

Number of Cloacal Contacts (count) - Day 1 2.339(0.439) 0.569/13.5 0.125/0.1 (0.478,4.201) Number of Cloacal Contacts (count) - Day 2 3.075(0.760) 1.408/12.8 0.644/0.3 (0.042,6.108) Number of Cloacal Contacts (count) - Day 3 4.203(1.391) 0.989/10.6 4.921/1.4 (0.000,8.766) 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 1.811(1.178) 1.188/12.7 3.113/1.1 (-2.061,5.683) 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 4.859(2.149) 0.905/11.2 12.987/1.8 (0.000,12.567Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 2.863(1.108) 0.726/8.9 3.081/1.4 (0.000,6.476) Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 3.425(1.994) 0.578/8.2 11.380/1.8 (0.000,10.809

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 to Day 3 -1.460(1.331) 1.442/10.1 4.142/1.2 (-5.819,2.900) 

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. For variables other than the change in length or change in number, the lower limits of the intervals are truncated at 0 since no measurement could be negative
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Table 7. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Interv

and Between Laboratories, F1 Generation 

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Thyroid Weight

15 Day Embryo (ED15) Thyroid Weight (mg) 1.795(0.417) 0.028/38.6 0.494/1.8 (0.269,3.320) 

Adult Left Thyroid Weight (mg) - Males 7.345(1.284) 0.684/39.6 4.285/1.5 (3.098,11.592) 

Adult Left Thyroid Weight (mg) - Females 11.506(2.812) 1.861/29.4 21.932/1.7 (1.654,21.358) 

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) 53.085(2.789) 27.291/39.0 0.000/1.0 (41.238,64.933) 

Bio Chem 

Egg Yolk Estradiol (pg/g) 67.845(13.405) 157.123/11.5 384.257/1.0 (23.204,112.486

Egg Yolk Testosterone (pg/g) 3041.891(987.311) 218993.8/5.8 2714558/1.7 (0.000,6547.689

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.748(0.099) 0.008/7.5 0.023/1.2 (0.417,1.078) 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.815(0.059) 0.006/10.3 0.004/0.3 (0.593,1.038) 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 3.722(1.322) 1.087/9.2 4.230/1.3 (0.000,8.078) 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 2.195(0.310) 0.424/9.7 0.011/0.0 (0.621,3.768) 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 5.569(0.727) 10.144/6.6 0.000/1.0 (0.000,13.240) 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 16.668(1.810) 11.153/8.8 2.081/0.1 (8.326,25.011) 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 1759.598(688.531) 130908.3/6.4 1301403/2.5 (0.000,3785.229

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 385.548(51.490) 20647.07/7.9 0.000/1.0 (45.184,725.912

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 
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Table 8. Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Random Laboratory 

Generationd

Parameter 

Individual Laboratory Mean (Standard Error)a Average A

Lab1_ST Lab2_SV Lab3_WLI Mean (Standard Error) b 

Body Weight 

Mean Offspring Body Weight by Pen (g) Day 0 8.494 (0.177) 9.150 (0.175) 6.544 (0.147) 8.060 (0.784) 

Mean Offspring Body Weight by Pen (g) Day 14 82.617 (1.543) 70.372 (1.357) 49.563 (1.045) 67.491 (9.653) 

Mating Behavior 

Length of Time to First Neck Grab (sec) - Day 1 NA 52.143 (41.928) 48.286 (42.004) 50.218 (29.674) Length of Time to First Neck Grab (sec) - Day 2 NA 44.714 (42.549) 33.286 (29.624) 37.017 (24.312) Length of Time to First Neck Grab (sec) - Day 3 NA 44.857 (42.525) 46.571 (42.272) 45.719 (29.980) 

Change in Length of Time to First Neck Grab (sec) from Day 1 

to Day 3 NA -7.286 (7.495) -1.714 (2.427) -2.243 (2.309) 

Length of Time to First Mount (sec) - Day 1 NA 57.143 (41.130) 54.286 (41.221) 55.717 (29.115) Length of Time to First Mount (sec) - Day 2 NA 45.857 (42.358) 89.286 (54.415) 62.243 (33.425) Length of Time to First Mount (sec) - Day 3 NA 45.571 (42.406) 48.143 (42.043) 46.868 (29.856) 

Change in Length of Time to First Mount (sec) from Day 1 to 

Day 3 NA -11.571 (7.662) -6.143 (2.807) -6.785 (2.635) 

Number of Mount Attempts (count) - Day 1 NA 9.143 (2.176) 3.286 (0.778) 5.862 (2.907) Number of Mount Attempts (count) - Day 2 NA 11.571 (3.184) 4.714 (1.248) 7.517 (3.371) Number of Mount Attempts (count) - Day 3 NA 8.714 (1.948) 5.000 (1.558) 6.673 (1.848) 

Change in Number of Mount Attempts (count) from Day 1 to 

Day 3 NA -0.429 (2.298) 1.714 (1.672) 0.972 (1.352) 

Number of Cloacal Contacts (count) - Day 1 NA 3.143 (0.829) 2.000 (0.787) 2.542 (0.571) Number of Cloacal Contacts (count) - Day 2 NA 5.143 (1.487) 2.429 (0.751) 3.482 (1.323) Number of Cloacal Contacts (count) - Day 3 NA 4.429 (0.972) 2.286 (0.522) 3.200 (1.060) 

Change in Number of Cloacal Contacts (count) from Day 1 to 

Day 3 NA 1.286 (0.421) 0.286 (0.778) 1.000 (0.452) 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 NA 6.857 (2.262) 1.000 (0.309) 3.500 (2.897) Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 NA 6.000 (0.951) 0.714 (0.421) 3.288 (2.642) Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 NA 4.714 (0.865) 1.286 (0.522) 2.931 (1.713) 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from 

Day 1 to Day 3 NA -2.143 (2.632) 0.286 (0.360) 0.241 (0.356) 
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a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 

d. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were av
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Table 8. (Continued). Inter-laboratory Analysis for Avian Control Data: Means and Standard Errors with Rando

F2 Generationd

Parameter 

Individual Laboratory Mean (Standard Error)a Average A

Lab1_ST Lab2_SV Lab3_WLI Mean (Standard Error) b 

Thyroid Weight 

14-Day Old Left Thyroid Weight (mg) 4.794 (0.239) NA NA NA 

14-Day Old Left Thyroid Weight (mg) - Males NA 4.029 (0.344) 1.913 (0.218) 2.954 (1.057) 

14-Day Old Left Thyroid Weight (mg) - Females NA 2.567 (0.370) 2.080 (0.248) 2.245 (0.231) 

15 Day Embryo (ED15) Thyroid Weight (mg) 1.106 (0.088) 1.869 (0.218) 3.286 (0.324) 2.057 (0.632) 

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) 51.580 (3.791) 53.701 (4.432) 41.527 (4.203) 48.939 (3.690) 

Bio Chem 

Plasma Thyroid Hormone T3 - 14-Day Old Males (ng/mL) 0.769 (0.061) 0.572 (0.092) 1.503 (0.114) 0.943 (0.280) 

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL) 0.817 (0.241) 1.232 (0.159) 2.333 (0.551) 1.320 (0.364) 

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL) 4.082 (0.833) 2.989 (1.293) 1.215 (0.292) 2.597 (0.956) 

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL) 3.760 (1.076) 2.660 (1.029) 2.449 (0.845) 2.864 (0.558) 

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) 2.884 (0.287) 5.747 (1.701) 4.918 (1.218) 4.016 (0.931) 

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) 12.116 (1.552) 9.570 (2.030) 14.803 (2.333) 11.992 (1.250) 

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL) 46.288 (11.184) 6.625 (1.167) 24.139 (4.080) 23.526 (10.830) 

Plasma Sex Hormone Testosterone - 14-Day Old Females 

(pg/mL) 26.237 (4.314) 4.680 (0.780) 31.051 (13.215) 18.332 (8.628) 

a. Means and their associated standard errors for each laboratory were pooled across all samples within the control groups for the laboratory. 

b. Averages and standard errors across laboratories were based on random effects model with laboratory treated as a random effect, incorporating heterogeneous 

c. CV = [(std err)K/Average]×100%, K is the number of laboratories with measurements. 

d. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were av
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Table 9. Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component Analysis with 

F2 Generationf

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (pvalues)c

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Body Weight 

Mean Offspring Body Weight by Pen (g) Day 0 0.031/17.0 0.031/17.0 0.022/15.0 0.028/48.5 1.816(<0.001) 

Mean Offspring Body Weight by Pen (g) Day 14 2.382/17.0 1.841/17.0 1.091/15.0 1.772/46.1 277.768(<0.001) 

Mating Behavior 

Length of Time to First Neck Grab (sec) - Day 1 NA 1757.925/6.0 1764.367/6.0 1761.146/12.0 0.000(1.000) Length of Time to First Neck Grab (sec) - Day 2 NA 1810.415/6.0 877.558/6.0 1343.986/10.7 0.000(1.000) Length of Time to First Neck Grab (sec) - Day 3 NA 1808.401/6.0 1786.946/6.0 1797.673/12.0 0.000(1.000) 

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 NA 56.177/6.0 5.891/6.0 31.034/7.2 0.000(1.000) 

Length of Time to First Mount (sec) - Day 1 NA 1691.687/6.0 1699.177/6.0 1695.432/12.0 0.000(1.000) Length of Time to First Mount (sec) - Day 2 NA 1794.163/6.0 2960.939/6.0 2377.551/11.3 0.000(1.000) Length of Time to First Mount (sec) - Day 3 NA 1798.279/6.0 1767.592/6.0 1782.935/12.0 0.000(1.000) 

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 NA 58.707/6.0 7.878/6.0 33.293/7.6 0.000(1.000) 

Number of Mount Attempts (count) - Day 1 NA 4.735/6.0 0.605/6.0 2.670/7.5 14.483(0.059) Number of Mount Attempts (count) - Day 2 NA 10.136/6.0 1.558/6.0 5.847/7.8 17.663(0.202) Number of Mount Attempts (count) - Day 3 NA 3.796/6.0 2.429/6.0 3.112/11.4 3.786(0.517) 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 NA 5.279/6.0 2.796/6.0 4.037/11.0 0.000(1.000) 

Number of Cloacal Contacts (count) - Day 1 NA 0.687/6.0 0.619/6.0 0.653/12.0 0.000(1.000) Number of Cloacal Contacts (count) - Day 2 NA 2.211/6.0 0.565/6.0 1.388/8.9 2.296(0.410) Number of Cloacal Contacts (count) - Day 3 NA 0.946/6.0 0.272/6.0 0.609/9.2 1.687(0.230) 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 NA 0.177/6.0 0.605/6.0 0.391/9.2 0.109(0.856) 

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 

d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance).

f. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were ava
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Table 9. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: Variance Component A

Laboratory, F2 Generationf

Parameter 

within Study Variance/Degree of Freedom (df)a Random Laboratoy 

Variances (p-values)c

Variance 

Average/d

Lab1_ST Lab2_SV Lab3_WLI Pooled withinb

Mating Behavior (Continued) 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 NA 5.116/6.0 0.095/6.0 2.605/6.2 14.548(0.054) 8.393/1.Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 NA 0.905/6.0 0.177/6.0 0.541/8.3 13.429(<0.001) 6.980/1.Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 NA 0.748/6.0 0.272/6.0 0.510/9.9 5.367(0.004) 2.934/1.

Change in Number of Crows (Cock) or Fluffs (Hen) (count) 

from Day 1 to Day 3 NA 6.925/6.0 0.129/6.0 3.527/6.2 0.000(1.000) 0.127/6.

Thyroid Weight

14-Day Old Left Thyroid Weight (mg) 0.057/16.0 NA NA NA NA NA 

14-Day Old Left Thyroid Weight (mg) - Males NA 0.118/13.0 0.048/14.0 0.083/22.2 2.154(<0.001) 1.118/1.

14-Day Old Left Thyroid Weight (mg) - Females NA 0.137/14.0 0.061/14.0 0.099/24.4 0.019(0.898) 0.053/1.

15 Day Embryo (ED15) Thyroid Weight (mg) 0.008/17.0 0.047/12.0 0.105/13.0 0.053/24.6 1.150(<0.001) 0.401/2.

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) 14.370/17.0 19.646/17.0 17.666/15.0 17.227/48.0 23.741(0.323) 13.617/2

Bio Chem 

Plasma Thyroid Hormone T3 - 14-Day Old Males (ng/mL) 0.004/4.0 0.008/4.0 0.013/4.0 0.008/10.0 0.227(<0.001) 0.079/2.

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL) 0.058/4.0 0.025/4.0 0.304/2.0 0.129/3.2 0.300(0.248) 0.133/2.

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL) 0.694/4.0 1.671/4.0 0.085/4.0 0.817/7.3 2.070(0.007) 0.915/2.

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL) 1.157/4.0 1.058/4.0 0.714/4.0 0.976/11.6 0.000(1.000) 0.312/11

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) 0.082/4.0 2.895/4.0 1.482/4.0 1.487/7.5 1.577(0.184) 0.867/2.

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) 2.408/4.0 4.122/4.0 5.443/4.0 3.991/10.9 1.027(0.843) 1.563/1.

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL) 125.088/3.0 1.361/4.0 16.646/4.0 47.698/3.9 311.835(<0.001) 117.289/2

Plasma Sex Hormone Testosterone - 14-Day Old Females 

(pg/mL) 18.609/4.0 0.608/4.0 174.635/4.0 64.617/4.9 179.356(<0.001) 74.443/2

a. The within study variance for each laboratory is the square of the standard error associated with the means, which was reported in Table 2. 

b. Pooled within is the unweighted average of the within-laboratory variances among the laboratories. Associated degrees of freedom is based on Satterthwaite’

c. Random laboratory variance is estimated using a random effects model with laboratory treated as a random effect, incorporating heterogeneous within-laborat

based on likelihood ratio test. 
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d. Variances for the averages were calculated as the square of the standard error of the pooled weighted averages value. It includes both within and among labor

freedom for the (mean) overall effect variance were estimated as 2*((1/K)*∑(SR2 + Si2))2/(var(SR2)+(2/K2)*∑(Si4/dfi)), where SR2 is the random laboratory vari

variance and degrees of freedom for laboratory i, var(SR2) is the variance of SR2, and K is the number of laboratories (Hartung and Makambi, 2001) 

e. P-value of the test of homogeneity of the within-laboratory variances based on an ANOVA model, applied to the log (within-laboratory variance). 

f. “NA” denotes that endpoint is not included in the study dataset for a laboratory, or that averages across laboratories were not calculated because data were ava
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Table 10. Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Intervals within La

Laboratories, F2 Generation 

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % 

Pooled withina Random Laboratoryb within Labora

Body Weight

Mean Offspring Body Weight by Pen (g) Day 0 8.060(0.784) 0.028/48.5 1.816/1.9 (4.877,11.2

Mean Offspring Body Weight by Pen (g) Day 14 67.491(9.653) 1.772/46.1 277.768/2.0 (27.015,107.

Mating Behavior

Length of Time to First Neck Grab (sec) - Day 1 50.218(29.674) 1761.146/12.0 0.000/1.0 (0.000,156.9Length of Time to First Neck Grab (sec) - Day 2 37.017(24.312) 1343.986/10.7 0.000/1.0 (0.000,129.2Length of Time to First Neck Grab (sec) - Day 3 45.719(29.980) 1797.673/12.0 0.000/1.0 (0.000,153.5

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 -2.243(2.309) 31.034/7.2 0.000/1.0 (-15.744,11.2

Length of Time to First Mount (sec) - Day 1 55.717(29.115) 1695.432/12.0 0.000/1.0 (0.000,160.4Length of Time to First Mount (sec) - Day 2 62.243(33.425) 2377.551/11.3 0.000/1.0 (0.000,185.5Length of Time to First Mount (sec) - Day 3 46.868(29.856) 1782.935/12.0 0.000/1.0 (0.000,154.2

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 -6.785(2.635) 33.293/7.6 0.000/1.0 (-20.809,7.2

Number of Mount Attempts (count) - Day 1 5.862(2.907) 2.670/7.5 14.483/0.7 (0.000,22.8Number of Mount Attempts (count) - Day 2 7.517(3.371) 5.847/7.8 17.663/0.6 (0.000,23.7Number of Mount Attempts (count) - Day 3 6.673(1.848) 3.112/11.4 3.786/0.3 (0.000,14.2

Change in Number of Mount Attempts (count) from Day 1 to Day 3 0.972(1.352) 4.037/11.0 0.000/1.0 (-4.093,6.03

Number of Cloacal Contacts (count) - Day 1 2.542(0.571) 0.653/12.0 0.000/0.0 (0.185,4.89Number of Cloacal Contacts (count) - Day 2 3.482(1.323) 1.388/8.9 2.296/0.4 (0.000,9.11Number of Cloacal Contacts (count) - Day 3 3.200(1.060) 0.609/9.2 1.687/0.5 (0.000,8.20

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 1.000(0.452) 0.391/9.2 0.109/0.0 (-0.881,2.8

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 3.500(2.897) 2.605/6.2 14.548/0.7 (0.000,20.5Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 3.288(2.642) 0.541/8.3 13.429/0.9 (0.000,28.5Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 2.931(1.713) 0.510/9.9 5.367/0.8 (0.000,15.6

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 to Day 3 0.241(0.356) 3.527/6.2 0.000/1.0 (-4.324,4.80

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 
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c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. For variables other than the change in length or change in number, the lower limits of the intervals are truncated at 0 since no measurement could be negative
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Table 10. (Continued). Inter-laboratory Analysis for Avian Control Data across All Studies: 95% Prediction Inter

Laboratory and Between Laboratories, F2 Generationf 

Parameter Average Mean (Std. Error) Variance (Degree of Freedom) 95 % P

Pooled withina Random Laboratoryb within Laborator

Thyroid Weight

14-Day Old Left Thyroid Weight (mg) NA NA NA NA 

14-Day Old Left Thyroid Weight (mg) - Males 2.954(1.057) 0.083/22.2 2.154/0.9 (0.000,13.179

14-Day Old Left Thyroid Weight (mg) - Females 2.245(0.231) 0.099/24.4 0.019/0.0 (1.327,3.164

15 Day Embryo (ED15) Thyroid Weight (mg) 2.051(0.633) 0.053/24.6 1.150/1.8 (0.000,4.414

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) 48.939(3.690) 17.227/48.0 23.741/0.7 (36.493,61.38

Bio Chem 

Plasma Thyroid Hormone T3 - 14-Day Old Males (ng/mL) 0.943(0.280) 0.008/10.0 0.227/1.9 (0.000,2.015

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL) 1.320(0.364) 0.129/3.2 0.300/1.1 (0.011,2.630

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL) 2.597(0.956) 0.817/7.3 2.070/1.1 (0.000,5.799

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL) 2.864(0.558) 0.976/11.6 0.000/1.0 (0.482,5.246

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) 4.016(0.931) 1.487/7.5 1.577/0.7 (0.540,7.491

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) 11.992(1.250) 3.991/10.9 1.027/0.1 (6.826,17.15

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL) 23.526(10.830) 47.698/3.9 311.835/1.6 (0.000,60.213

Plasma Sex Hormone Testosterone - 14-Day Old Females (pg/mL) 18.332(8.628) 64.617/4.9 179.356/1.3 (0.000,47.375

a. Pooled within is the unweighted average of the within-laboratory variances among the studies. Associated degrees of freedom is based on Satterthwaite’s app

b. Random laboratory variance is estimated using a random effects model with study and laboratory treated as random effects, incorporating heterogeneous with

model is applied to all six studies. Degree of freedom is calculated based on chi-square distribution. If random lab+study is smaller than random study, then 

c. The prediction interval for within-laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within )), where υ is degree of freedom as calc

approximation. 

d. The prediction interval for between laboratory is (average mean) ± t0.975,υ √((standard error of mean)^2 + (pooled within ) + random laboratory), where υ is de

using Satterthwaite’s approximation. 

e. The lower limits of the intervals are truncated at 0 since no measurement could be negative. 

f. “NA” denotes that averages across laboratories were not calculated because data were available for only one laboratory. 
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Table 11. Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Level. Avian Stud

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Body Weight 

Adult Body Weight (g) Day 0 - Males 168.849 3.347 18 13.652 

Adult Body Weight (g) Day 56 - Males 184.637 3.297 18 13.445 

Adult Body Weight (g) Day 0 - Females 207.898 5.334 18 21.753 

Adult Body Weight (g) Day 56 - Females 227.716 5.186 18 21.151 

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 26.402 0.678 18 2.766 Adult Feed Consumption (g/bird/day) - Week 2 26.199 0.932 18 3.800 Adult Feed Consumption (g/bird/day) - Week 3 25.203 0.784 18 3.197 Adult Feed Consumption (g/bird/day) - Week 4 26.791 0.909 18 3.708 Adult Feed Consumption (g/bird/day) - Week 5 29.183 0.862 18 3.515 Adult Feed Consumption (g/bird/day) - Week 6 28.979 0.790 18 3.222 Adult Feed Consumption (g/bird/day) - Week 7 28.524 0.695 18 2.836 Adult Feed Consumption (g/bird/day) - Week 8 26.849 0.556 18 2.268 

Reproduction 

Eggs Laid 42.897 1.759 18 7.175 

Eggs Crack 0.907 0.548 18 2.233 

Eggs Abnormal 1.269 0.797 18 3.252 

Eggs Set From Weeks 7 and 8 11.678 0.441 18 1.797 

Eggs Set From Weeks 6 7 and 8 17.440 0.656 18 2.677 

Eggs Fertile From Weeks 7 and 8 11.249 0.477 18 1.946 

Eggs Fertile From Weeks 6 7 and 8 16.867 0.706 18 2.881 

Eggs Viable From Weeks 7 and 8 10.915 0.472 18 1.927 

Eggs Viable From Weeks 6 7 and 8 16.202 0.702 18 2.864 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for ontrol group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 11. (Continued). Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Lev

Generation. 

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Reproduction (Continued) 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 10.841 0.471 18 1.921 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 16.106 0.705 18 2.874 

Eggs Hatched 9.907 0.542 18 2.212 

Number of 14-Day-Old Surviving Chicks 8.790 0.657 18 2.681 

Eggs Laid Per Hen Per Day 0.902 0.028 18 0.115 

Cracked Eggs as A Percentage of Eggs Laid 0.022 0.013 18 0.054 

Abnormal Eggs as A Percentage of Eggs Laid 0.032 0.021 18 0.085 

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.969 0.018 18 0.075 

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.970 0.016 18 0.064 

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.944 0.023 18 0.093 

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.935 0.023 18 0.093 

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as A Percentage of 

Viable Eggs From Weeks 7 and 8 0.995 0.006 18 0.023 

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as A Percentage of 

Viable Eggs From Weeks 6 7 and 8 0.996 0.004 18 0.018 

Eggs Hatched as A Percentage of Number of Viable Eggs from Weeks 7 and 8 after 

Two Weeks of Incubation 0.910 0.027 18 0.111 

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs Hatched 0.899 0.037 18 0.152 

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 and 8 0.852 0.034 18 0.140 

Eggs Hatched as A Percentage of Number of Fertile Eggs from Weeks 7 and 8 0.881 0.031 18 0.126 

Number of 14-Day-Old Surviving Chicks as A Percentage of Number of Eggs Set from 

Weeks 7 and 8 0.768 0.047 18 0.192 

Eggs Hatched Per Hen Per Day 0.708 0.039 18 0.158 

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.627 0.047 18 0.192 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 11. (Continued). Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Lev

Generation. 

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.215 0.003 18 0.014 

Pen Mean Egg Content Weight - Albumin (g) 4.743 0.164 18 0.670 

Pen Mean Egg Content Weight - Yolk (g) 3.640 0.131 18 0.535 

Pen Mean Egg Content Weight - Combined (g) 8.394 0.261 18 1.065 

Thyroid Weight 

Adult Left Thyroid Weight (mg) – Males 7.348 0.972 18 3.964 

Adult Left Thyroid Weight (mg) – Females 9.096 0.897 18 3.658 

Bio Chem 

Egg Yolk Estradiol (pg/g) 44.421 4.811 5 21.778 

Egg Yolk Testosterone (pg/g) 2050.636 501.036 5 2268.08

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.871 0.122 5 0.554 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.860 0.082 5 0.370 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 3.164 0.839 5 3.799 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 1.763 0.706 5 3.194 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 4.611 0.984 5 4.452 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 18.452 2.622 5 11.870 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 1694.232 366.865 5 1660.71

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 337.621 203.841 5 922.74

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 12. Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Level. Avian Stud

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Body Weight 

Adult Body Weight (g) Day 0 - Males 138.313 2.696 18 10.995 

Adult Body Weight (g) Day 56 - Males 182.906 4.003 18 16.326 

Adult Body Weight (g) Day 0 - Females 149.912 3.982 18 16.242 

Adult Body Weight (g) Day 56 - Females 228.910 5.856 18 23.883 

Mean Offspring Body Weight by Pen (g) Day 0 8.365 0.171 18 0.698 

Mean Offspring Body Weight by Pen (g) Day 14 70.035 1.180 18 4.811 

Feed Consumption

Adult Feed Consumption (g/bird/day) - Week 1 27.262 0.618 18 2.520 Adult Feed Consumption (g/bird/day) - Week 2 29.728 0.663 18 2.706 Adult Feed Consumption (g/bird/day) - Week 3 28.659 0.883 18 3.600 Adult Feed Consumption (g/bird/day) - Week 4 26.854 0.789 18 3.218 Adult Feed Consumption (g/bird/day) - Week 5 26.431 0.843 18 3.440 Adult Feed Consumption (g/bird/day) - Week 6 27.453 0.846 18 3.452 Adult Feed Consumption (g/bird/day) - Week 7 28.391 0.681 18 2.777 Adult Feed Consumption (g/bird/day) - Week 8 27.657 0.967 18 3.945 Adult Feed Consumption (g/bird/day) - Week 9 31.400 0.993 18 4.049 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 12. (Continued). Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Lev

Generation. 

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Reproduction 

Eggs Laid 43.527 1.543 18 6.293 

Eggs Crack 1.014 0.382 18 1.560 

Eggs Abnormal 1.304 0.531 18 2.167 

Eggs Set From Weeks 7 and 8 11.447 0.548 18 2.235 

Eggs Set From Weeks 6 7 and 8 24.007 0.991 18 4.042 

Eggs Fertile From Weeks 7 and 8 10.954 0.683 18 2.786 

Eggs Fertile From Weeks 6 7 and 8 23.020 1.137 18 4.637 

Eggs Viable From Weeks 7 and 8 12.528 0.775 18 3.159 

Eggs Viable From Weeks 6 7 and 8 22.290 1.201 18 4.899 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 12.377 0.797 18 3.252 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 15.873 0.981 18 4.003 

Eggs Hatched 10.100 0.776 18 3.166 

Number of 14-Day-Old Surviving Chicks 9.676 0.768 18 3.131 

Eggs Laid Per Hen Per Day 0.889 0.031 18 0.128 

Cracked Eggs as A Percentage of Eggs Laid 0.025 0.010 18 0.041 

Abnormal Eggs as A Percentage of Eggs Laid 0.033 0.015 18 0.063 

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.970 0.033 18 0.133 

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.959 0.021 18 0.086 

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.945 0.032 18 0.131 

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.927 0.027 18 0.112 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 12. (Continued). Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Lev

Generation. 

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Reproduction (Continued) 

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as A Percentage of 

Viable Eggs From Weeks 7 and 8 0.995 0.010 18 0.042 

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as A Percentage of 

Viable Eggs From Weeks 6 7 and 8 0.997 0.010 18 0.039 

Eggs Hatched as A Percentage of Number of Viable Eggs from Weeks 7 and 8 after 

Two Weeks of Incubation 0.944 0.030 18 0.124 

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs Hatched 0.964 0.016 18 0.066 

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 and 8 0.866 0.047 18 0.192 

Eggs Hatched as A Percentage of Number of Fertile Eggs from Weeks 7 and 8 0.913 0.037 18 0.150 

Number of 14-Day-Old Surviving Chicks as A Percentage of Number of Eggs Set from 

Weeks 7 and 8 0.834 0.048 18 0.197 

Eggs Hatched Per Hen Per Day 0.722 0.056 18 0.227 

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.691 0.055 18 0.224 

Egg Shell and Weight 

Pen Mean Egg Shell Thickness (mm) 0.213 0.003 18 0.013 

Pen Mean Egg Content Weight - Albumin (g) 5.257 0.154 18 0.626 

Pen Mean Egg Content Weight - Yolk (g) 3.626 0.094 18 0.385 

Pen Mean Egg Content Weight - Combined (g) 8.881 0.218 18 0.890 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 12. (Continued). Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Lev

Generation. 

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

First Foam First Egg Cloacal Size 

Age in Days at First Foam - Males 37.227 2.037 18 8.306 

Age in Days at First Egg - Females 44.964 1.303 18 5.314 

Cloacal Area (cm2) at 4 Weeks - Males 0.255 0.020 18 0.080 Cloacal Area (cm2) at 5 Weeks - Males 0.384 0.049 18 0.198 Cloacal Area (cm2) at 6 Weeks - Males 0.546 0.033 18 0.135 

Cloacal Area (cm2) at 4 Weeks - Females 0.249 0.024 18 0.097 Cloacal Area (cm2) at 5 Weeks - Females 0.336 0.058 18 0.237 Cloacal Area (cm2) at 6 Weeks - Females 0.515 0.073 18 0.299 

Mating Behavior 

Length of Time to First Neck Grab (sec) - Day 1 10.208 7.870 7 33.976 Length of Time to First Neck Grab (sec) - Day 2 6.393 2.037 7 8.792 Length of Time to First Neck Grab (sec) - Day 3 3.118 0.668 7 2.885 

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 -6.676 7.491 7 32.338 

Length of Time to First Mount (sec) - Day 1 44.713 34.513 7 148.99Length of Time to First Mount (sec) - Day 2 14.351 24.354 7 105.14Length of Time to First Mount (sec) - Day 3 5.256 6.652 7 28.716 

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 -29.801 34.040 7 146.95

Number of Mount Attempts (count) - Day 1 6.055 1.134 7 4.895 Number of Mount Attempts (count) - Day 2 7.405 2.401 7 10.367 Number of Mount Attempts (count) - Day 3 7.425 2.129 7 9.191 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 1.896 1.904 7 8.218 

Number of Cloacal Contacts (count) - Day 1 2.339 0.754 7 3.257 Number of Cloacal Contacts (count) - Day 2 3.075 1.187 7 5.123 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 12. (Continued). Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Lev

Generation. 

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Mating Behavior (Continued) 

Number of Cloacal Contacts (count) - Day 3 4.203 0.994 7 4.293 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 1.811 1.090 7 4.706 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 4.859 0.951 7 4.106 Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 2.863 0.852 7 3.677 Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 3.425 0.760 7 3.283 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 to Day 3 -1.460 1.201 7 5.184 

Thyroid Weight 

15 Day Embryo (ED15) Thyroid Weight (mg) 1.795 0.168 18 0.687 

Adult Left Thyroid Weight (mg) - Males 7.345 0.827 18 3.372 

Adult Left Thyroid Weight (mg) - Females 11.506 1.364 18 5.564 

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) 53.085 5.224 18 21.306 

Bio Chem 

Egg Yolk Estradiol (pg/g) 67.845 12.535 5 56.743 

Egg Yolk Testosterone (pg/g) 3041.891 467.968 5 2118.39

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.748 0.090 5 0.407 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.815 0.080 5 0.364 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 3.722 1.043 5 4.720 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 2.195 0.652 5 2.949 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 5.569 3.185 5 14.418 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 16.668 3.340 5 15.118 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 1759.598 361.813 5 1637.84

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 385.548 143.691 5 650.45

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
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Table 13. Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Level. Avian Stud

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Body Weight 

Mean Offspring Body Weight by Pen (g) Day 0 8.060 0.167 18 0.681 

Mean Offspring Body Weight by Pen (g) Day 14 67.491 1.331 18 5.428 

Mating Behavior 

Length of Time to First Neck Grab (sec) - Day 1 50.218 41.966 7 181.17Length of Time to First Neck Grab (sec) - Day 2 37.017 36.660 7 158.26Length of Time to First Neck Grab (sec) - Day 3 45.719 42.399 7 183.04

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 -2.243 5.571 7 24.050 

Length of Time to First Mount (sec) - Day 1 55.717 41.176 7 177.76Length of Time to First Mount (sec) - Day 2 62.243 48.760 7 210.50Length of Time to First Mount (sec) - Day 3 46.868 42.225 7 182.28

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 -6.785 5.770 7 24.910 

Number of Mount Attempts (count) - Day 1 5.862 1.634 7 7.054 Number of Mount Attempts (count) - Day 2 7.517 2.418 7 10.439 Number of Mount Attempts (count) - Day 3 6.673 1.764 7 7.616 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 0.972 2.009 7 8.675 

Number of Cloacal Contacts (count) - Day 1 2.542 0.808 7 3.489 Number of Cloacal Contacts (count) - Day 2 3.482 1.178 7 5.086 Number of Cloacal Contacts (count) - Day 3 3.200 0.780 7 3.369 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 1.000 0.625 7 2.700 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 3.500 1.614 7 6.968 Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 3.288 0.735 7 3.175 Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 2.931 0.714 7 3.084 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 to Day 3 0.241 1.878 7 8.108 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

60 

Table 13. (Continued). Alternatives that can be Detected with 80% Power at the 5% (Two-Sided) Significance Lev

Generation. 

Parameter Mean (Control)a Standard Error (Control)b Sample Size Per Groupc Detectio

Distanc

Thyroid Weight 

14-Day Old Left Thyroid Weight (mg) - Males 2.954 0.288 18 1.175 

14-Day Old Left Thyroid Weight (mg) - Females 2.245 0.315 18 1.285 

15 Day Embryo (ED15) Thyroid Weight (mg) 2.051 0.231 18 0.941 

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) 48.939 4.151 18 16.928 

Bio Chem 

Plasma Thyroid Hormone T3 - 14-Day Old Males (ng/mL) 0.943 0.091 5 0.413 

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL) 1.320 0.359 5 1.626 

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL) 2.597 0.904 5 4.091 

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL) 2.864 0.988 5 4.473 

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) 4.016 1.219 5 5.519 

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) 11.992 1.998 5 9.044 

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL) 23.526 6.906 5 31.264 

Plasma Sex Hormone Testosterone - 14-Day Old Females (pg/mL) 18.332 8.038 5 36.389 

a. Weighted mean across studies for control group as presented in the interlaboratory analysis of control data. b. Square root of pooled within run variances for control group as presented in the inter-lab analysis of control data. 

c. Control group sample size and projected sample sizes per concentration group. 

d. Detection distance for declaring a significant difference from the control group at the 5% level of sgnioficance with 80% power. 

e. A concentration group mean value below the lower limit or above the upper limit will be declared to be significantly different from control group at the 5% le
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

61 

Table 14. Mean and Standard Errors and Comparisons of Avian Vinclozolin Concentrations to 

Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Body Weight

Adult Body Weight (g) 

Day 0 - Males 

Lab1_ST 190.661 

(3.360) 

188.739 

(3.562) 

185.778 

(3.460) 

185.978 

(4.310) 

188.839 

(3.113) 

Lab2_SV 202.739 

(4.357) 

216.361 

(4.995) 

207.539 

(5.094) 

213.744 

(4.201) 

210.300 

(4.679) 

Lab3_WLI 113.444 

(1.828) 

112.889 

(1.569) 

112.389 

(1.966) 

111.944 

(1.850) 

113.000 

(1.768) 

Average 170.418 

(29.524) 

170.995 

(29.520) 

169.294 

(29.530) 

169.966 

(29.528) 

170.660 

(29.522) 

Adult Body Weight (g) 

Day 56 - Males 

Lab1_ST 210.625 

(3.920) 

211.728 

(4.982) 

198.656 

(5.079) 

200.324 

(4.438) 

208.712 

(4.390) 

Lab2_SV 221.482 

(3.807) 

230.844 

(4.685) 

229.672 

(4.657) 

227.856 

(4.939) 

227.583 

(3.917) 

Lab3_WLI 122.059 

(1.657) 

118.000 

(2.121) 

120.059 

(3.239) 

116.824 

(2.187) 

118.667 

(2.071) 

Average 185.552 

(33.100) 

185.168 

(33.121) 

183.514 

(33.145) 

181.587 

(33.120) 

184.483 

(33.110) 

Adult Body Weight (g) 

Day 0 - Females 

Lab1_ST 233.567 

(6.594) 

234.439 

(5.726) 

240.533 

(4.037) 

227.017 

(3.876) 

224.583 

(3.003) 

Lab2_SV 247.556 

(5.955) 

243.661 

(3.550) 

239.289 

(5.168) 

248.828 

(3.597) 

247.478 

(4.699) 

Lab3_WLI 143.222 

(2.529) 

140.611 

(2.493) 

138.611 

(2.193) 

144.222 

(2.495) 

139.222 

(2.385) 

Average 208.083 

(32.851) 

205.676 

(32.815) 

206.070 

(32.812) 

207.094 

(32.800) 

203.177 

(32.799) 

Adult Body Weight (g) 

Day 56 - Females 

Lab1_ST 249.444 

(5.737) 

265.483 

(6.425) 

256.788 

(6.023) 

260.335 

(4.804) 

258.141 

(5.463) 

Lab2_SV 276.924 

(6.406) 

266.183 

(5.181) 

270.422 

(5.779) 

274.394 

(4.995) 

277.317 

(6.331) 

Lab3_WLI 157.353 

(2.598) 

154.000 

(1.843) 

154.176 

(1.770) 

159.471 

(5.204) 

153.278 

(3.481) 

Average 229.101 

(36.786) 

227.055 

(36.764) 

227.067 

(36.763) 

231.372 

(36.822) 

228.150 

(36.810) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

62 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Feed Consumption

Adult Feed Consumption 

(g/bird/day) - Week 1

Lab1_ST 29.611 

(0.600) 

29.111 

(0.804) 

28.778 

(1.119) 

29.000 

(0.820) 

26.389 

(1.088) 

Lab2_SV 31.033 

(0.722) 

32.144 

(1.014) 

32.661 

(1.042) 

31.978 

(0.734) 

30.706 

(0.953) 

Lab3_WLI 18.556 

(0.706) 

19.529 

(0.576) 

17.944 

(0.574) 

18.056 

(0.375) 

17.667 

(0.388) 

Average 26.482 

(4.052) 

27.066 

(4.057) 

26.199 

(4.062) 

26.155 

(4.048) 

25.343 

(4.052) 

Adult Feed Consumption 

(g/bird/day) - Week 2 

Lab1_ST 29.667 

(0.828) 

31.611 

(1.004) 

30.824 

(0.744) 

29.056 

(0.898) 

27.941 

(1.016) 

Lab2_SV 28.278 

(1.198) 

30.800 

(0.761) 

30.594 

(1.062) 

30.128 

(0.716) 

29.161 

(1.141) 

Lab3_WLI 20.765 

(0.694) 

20.765 

(0.407) 

19.611 

(0.662) 

19.611 

(0.421) 

19.944 

(0.347) 

Average 26.558 

(3.300) 

27.506 

(3.284) 

26.896 

(3.294) 

26.255 

(3.283) 

26.088 

(3.287) 

Adult Feed Consumption 

(g/bird/day) - Week 3 

Lab1_ST 28.944 

(0.782) 

30.667 

(0.929) 

28.059 

(1.005) 

29.056 

(0.850) 

27.118 

(0.624) 

Lab2_SV 27.556 

(1.022) 

28.256 

(0.538) 

29.550 

(1.358) 

28.906 

(0.689) 

27.239 

(0.756) 

Lab3_WLI 19.235 

(0.433) 

19.294 

(0.444) 

19.111 

(0.599) 

19.389 

(0.293) 

19.778 

(0.367) 

Average 25.278 

(3.058) 

25.798 

(3.051) 

25.323 

(3.076) 

25.690 

(3.050) 

24.880 

(3.048) 

Adult Feed Consumption 

(g/bird/day) - Week 4 

Lab1_ST 30.824 

(1.340) 

33.667 

(0.953) 

29.176 

(1.207) 

30.111 

(1.029) 

30.765 

(0.603) 

Lab2_SV 28.524 

(0.693) 

30.739 

(0.645) 

31.044 

(0.632) 

31.978 

(0.745) 

29.861 

(1.017) 

Lab3_WLI 21.294 

(0.452) 

20.588 

(0.462) 

20.056 

(0.563) 

20.944 

(0.408) 

21.500 

(0.326) 

Average 26.868 

(3.350) 

28.116 

(3.341) 

26.913 

(3.349) 

27.729 

(3.344) 

27.471 

(3.339) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

63 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Feed Consumption (Continued)

Adult Feed Consumption 

(g/bird/day) - Week 5 

Lab1_ST 33.438 

(1.235) 

32.944 

(0.998) 

29.375 

(1.500) 

30.722 

(1.047) 

29.706 

(0.690) 

Lab2_SV 31.418 

(0.696) 

31.406 

(0.578) 

32.328 

(0.504) 

32.922 

(0.585) 

31.611 

(0.940) 

Lab3_WLI 22.882 

(0.469) 

22.412 

(0.454) 

22.444 

(0.633) 

22.056 

(0.439) 

22.500 

(0.373) 

Average 28.928 

(3.098) 

28.670 

(3.091) 

28.463 

(3.101) 

28.613 

(3.092) 

28.009 

(3.090) 

Adult Feed Consumption 

(g/bird/day) - Week 6 

Lab1_ST 35.750 

(1.078) 

34.833 

(1.167) 

32.375 

(1.036) 

30.000 

(1.512) 

30.765 

(0.902) 

Lab2_SV 29.382 

(0.708) 

30.139 

(0.891) 

29.844 

(1.004) 

31.344 

(0.424) 

30.522 

(0.760) 

Lab3_WLI 21.941 

(0.458) 

22.176 

(0.564) 

21.882 

(0.629) 

21.471 

(0.333) 

21.944 

(0.818) 

Average 28.782 

(3.401) 

28.918 

(3.411) 

28.061 

(3.412) 

28.079 

(3.402) 

27.787 

(3.407) 

Adult Feed Consumption 

(g/bird/day) - Week 7 

Lab1_ST 32.375 

(0.758) 

33.778 

(1.330) 

33.063 

(0.964) 

32.471 

(1.237) 

29.647 

(0.849) 

Lab2_SV 30.282 

(0.674) 

32.511 

(0.692) 

32.072 

(0.557) 

32.533 

(0.583) 

31.511 

(0.681) 

Lab3_WLI 22.941 

(0.650) 

22.647 

(0.514) 

22.941 

(0.689) 

22.176 

(0.637) 

22.500 

(0.466) 

Average 28.516 

(3.147) 

29.366 

(3.154) 

29.292 

(3.150) 

29.045 

(3.154) 

28.084 

(3.145) 

Adult Feed Consumption 

(g/bird/day) - Week 8 

Lab1_ST NA NA NA NA NA 

Lab2_SV 30.076 

(0.625) 

31.494 

(0.541) 

32.400 

(0.594) 

32.111 

(0.747) 

30.261 

(0.634) 

Lab3_WLI 23.647 

(0.477) 

22.647 

(0.581) 

23.059 

(0.745) 

22.938 

(0.744) 

23.833 

(0.622) 

Average 26.922 

(4.043) 

27.081 

(4.044) 

27.790 

(4.052) 

27.523 

(4.059) 

27.052 

(4.049) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

64 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Feed Consumption (Continued)

Adult Feed Consumption 

(g/bird/day) - Week 9 

Lab1_ST NA NA NA NA NA 

Lab2_SV 34.418 

(0.838) 

36.428 

(1.137) 

38.467 

(0.753) 

35.833 

(0.760) 

27.822 

(0.754) 

Lab3_WLI NA NA NA NA NA 

Average NA NA NA NA NA 

Reproduction

Eggs Laid 

Lab1_ST 39.750 

(1.445) 

34.722 

(3.342) 

36.813 

(2.608) 

36.333 

(2.882) 

39.588 

(1.018) 

Lab2_SV 44.056 

(2.509) 

44.000 

(1.328) 

42.444 

(2.610) 

46.111 

(0.565) 

40.667 

(2.926) 

Lab3_WLI 44.941 

(0.949) 

44.765 

(1.146) 

41.294 

(1.497) 

43.824 

(1.079) 

43.333 

(1.207) 

Average 43.294 

(1.954) 

42.445 

(1.991) 

40.111 

(2.139) 

43.395 

(1.900) 

42.225 

(1.956) 

Eggs Crack 

Lab1_ST 1.500 

(0.532) 

0.529 

(0.259) 

0.438 

(0.223) 

1.000 

(0.256) 

1.824 

(0.660) 

Lab2_SV 1.611 

(0.776) 

1.778 

(0.693) 

1.222 

(0.552) 

0.722 

(0.351) 

1.111 

(0.361) 

Lab3_WLI 0.118 

(0.118) 

0.000 

(0.000) 

0.118 

(0.081) 

0.412 

(0.354) 

0.111 

(0.076) 

Average 0.701 

(0.324) 

0.503 

(0.303) 

0.552 

(0.305) 

0.739 

(0.333) 

0.665 

(0.310) 

Eggs Abnormal 

Lab1_ST 1.938 

(0.710) 

0.765 

(0.219) 

2.125 

(1.136) 

1.167 

(0.422) 

0.647 

(0.191) 

Lab2_SV 2.444 

(1.161) 

2.000 

(0.681) 

1.778 

(0.684) 

0.889 

(0.395) 

2.333 

(0.754) 

Lab3_WLI 0.235 

(0.235) 

0.529 

(0.529) 

0.176 

(0.128) 

0.059 

(0.059) 

0.111 

(0.076) 

Average 1.005 

(0.445) 

0.854 

(0.433) 

0.839 

(0.408) 

0.694 

(0.394) 

0.739 

(0.394) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

65 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Reproduction (Continued)

Eggs Set From Weeks 7 

and 8 

Lab1_ST 10.438 

(0.626) 

10.118 

(1.011) 

9.438 

(0.940) 

10.941 

(0.740) 

10.471 

(0.550) 

Lab2_SV 11.941 

(0.397) 

11.167 

(0.628) 

11.882 

(0.208) 

12.056 

(0.221) 

11.375 

(0.523) 

Lab3_WLI 12.235 

(0.182) 

12.059 

(0.518) 

11.824 

(0.246) 

11.412 

(0.486) 

11.722 

(0.477) 

Average 11.702 

(0.495) 

11.219 

(0.592) 

11.393 

(0.492) 

11.543 

(0.507) 

11.189 

(0.544) 

Eggs Set From Weeks 6 7 

and 8 

Lab1_ST 15.375 

(0.957) 

15.294 

(1.414) 

14.188 

(1.339) 

16.118 

(1.218) 

15.706 

(0.780) 

Lab2_SV 18.059 

(0.532) 

16.556 

(0.933) 

17.059 

(0.539) 

17.889 

(0.312) 

16.235 

(1.139) 

Lab3_WLI 18.294 

(0.306) 

18.294 

(0.721) 

17.353 

(0.500) 

17.294 

(0.513) 

17.611 

(0.572) 

Average 17.472 

(0.742) 

16.915 

(0.868) 

16.483 

(0.778) 

17.135 

(0.748) 

16.739 

(0.808) 

Eggs Fertile From Weeks 

7 and 8 

Lab1_ST 9.813 

(0.634) 

11.133 

(0.477) 

9.133 

(0.960) 

10.938 

(0.413) 

10.118 

(0.618) 

Lab2_SV 11.706 

(0.435) 

10.722 

(0.690) 

11.353 

(0.428) 

11.389 

(0.506) 

11.188 

(0.647) 

Lab3_WLI 11.941 

(0.303) 

11.353 

(0.878) 

11.294 

(0.520) 

10.529 

(0.777) 

11.333 

(0.505) 

Average 11.423 

(0.366) 

11.218 

(0.456) 

10.942 

(0.421) 

11.161 

(0.407) 

10.900 

(0.432) 

Eggs Fertile From Weeks 

6 7 and 8 

Lab1_ST 14.375 

(0.999) 

14.588 

(1.458) 

13.400 

(1.290) 

16.063 

(0.824) 

14.824 

(0.904) 

Lab2_SV 17.824 

(0.570) 

16.056 

(0.958) 

16.471 

(0.796) 

17.000 

(0.686) 

16.059 

(1.220) 

Lab3_WLI 17.941 

(0.415) 

17.588 

(1.036) 

16.529 

(0.883) 

16.294 

(0.911) 

17.111 

(0.582) 

Average 17.055 

(0.761) 

16.083 

(0.932) 

15.665 

(0.871) 

16.512 

(0.820) 

16.162 

(0.825) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

66 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Reproduction (Continued)

Eggs Viable From Weeks 

7 and 8 

Lab1_ST 9.188 

(0.621) 

10.733 

(0.521) 

9.286 

(0.835) 

10.188 

(0.493) 

9.706 

(0.652) 

Lab2_SV 11.647 

(0.428) 

10.389 

(0.647) 

11.059 

(0.433) 

11.167 

(0.606) 

10.938 

(0.642) 

Lab3_WLI 11.706 

(0.318) 

11.176 

(0.863) 

10.529 

(0.718) 

9.941 

(0.872) 

10.278 

(0.709) 

Average 11.068 

(0.463) 

10.801 

(0.540) 

10.428 

(0.529) 

10.569 

(0.530) 

10.318 

(0.545) 

Eggs Viable From Weeks 

6 7 and 8 

Lab1_ST 13.000 

(0.979) 

14.813 

(1.177) 

13.643 

(0.947) 

15.063 

(0.920) 

14.118 

(0.996) 

Lab2_SV 17.647 

(0.555) 

15.500 

(0.923) 

16.059 

(0.783) 

16.500 

(0.829) 

15.706 

(1.233) 

Lab3_WLI 17.647 

(0.461) 

17.294 

(1.017) 

15.529 

(1.029) 

15.471 

(1.154) 

17.111 

(0.582) 

Average 16.520 

(0.907) 

15.766 

(1.023) 

15.142 

(0.984) 

15.826 

(0.997) 

15.879 

(0.969) 

Viable Eggs From Weeks 

7 and 8 after Two Weeks 

of Incubation 

Lab1_ST 9.188 

(0.621) 

10.733 

(0.521) 

9.286 

(0.835) 

10.188 

(0.493) 

9.706 

(0.652) 

Lab2_SV 11.529 

(0.412) 

10.389 

(0.647) 

10.765 

(0.518) 

10.833 

(0.617) 

10.875 

(0.638) 

Lab3_WLI 11.588 

(0.333) 

11.000 

(0.853) 

10.294 

(0.736) 

9.941 

(0.872) 

10.278 

(0.709) 

Average 11.011 

(0.411) 

10.754 

(0.495) 

10.215 

(0.505) 

10.445 

(0.487) 

10.298 

(0.502) 

Viable Eggs From Weeks 

6 7 and 8 after Two Weeks 

of Incubation 

Lab1_ST 13.000 

(0.979) 

14.813 

(1.177) 

13.643 

(0.947) 

15.063 

(0.920) 

14.118 

(0.996) 

Lab2_SV 17.529 

(0.550) 

15.500 

(0.923) 

15.765 

(0.860) 

16.167 

(0.849) 

15.647 

(1.231) 

Lab3_WLI 17.471 

(0.478) 

16.941 

(1.005) 

15.294 

(1.049) 

15.471 

(1.154) 

15.611 

(0.922) 

Average 16.492 

(0.781) 

15.673 

(0.911) 

14.933 

(0.882) 

15.660 

(0.886) 

15.124 

(0.914) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

67 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Reproduction (Continued)

Eggs Hatched 

Lab1_ST 7.750 

(0.668) 

10.067 

(0.589) 

8.429 

(0.837) 

8.938 

(0.470) 

9.059 

(0.704) 

Lab2_SV 10.882 

(0.469) 

9.722 

(0.779) 

9.824 

(0.768) 

9.889 

(0.820) 

10.313 

(0.669) 

Lab3_WLI 10.941 

(0.466) 

10.471 

(0.900) 

10.176 

(0.739) 

9.765 

(0.902) 

10.111 

(0.732) 

Average 10.054 

(0.568) 

10.208 

(0.642) 

9.494 

(0.658) 

9.589 

(0.624) 

9.835 

(0.628) 

Number of 14-Day-Old 

Surviving Chicks 

Lab1_ST 7.688 

(0.663) 

9.800 

(0.595) 

7.143 

(0.748) 

8.438 

(0.547) 

8.941 

(0.694) 

Lab2_SV 9.706 

(0.400) 

9.500 

(0.764) 

10.125 

(0.515) 

10.353 

(0.630) 

10.000 

(0.695) 

Lab3_WLI 8.706 

(0.835) 

8.294 

(0.882) 

7.824 

(0.754) 

7.647 

(0.870) 

7.667 

(0.691) 

Average 8.613 

(0.693) 

9.363 

(0.736) 

8.514 

(0.712) 

8.877 

(0.714) 

8.868 

(0.724) 

Eggs Laid Per Hen Per 

Day 

Lab1_ST 0.811 

(0.029) 

0.750 

(0.057) 

0.751 

(0.053) 

0.742 

(0.059) 

0.808 

(0.021) 

Lab2_SV 0.980 

(0.033) 

0.899 

(0.027) 

0.868 

(0.053) 

0.942 

(0.011) 

0.831 

(0.060) 

Lab3_WLI 0.917 

(0.019) 

0.914 

(0.023) 

0.843 

(0.031) 

0.894 

(0.022) 

0.884 

(0.025) 

Average 0.899 

(0.042) 

0.869 

(0.043) 

0.821 

(0.046) 

0.884 

(0.041) 

0.868 

(0.042) 

Cracked Eggs as A 

Percentage of Eggs Laid 

Lab1_ST 0.039 

(0.013) 

0.018 

(0.008) 

0.014 

(0.008) 

0.046 

(0.019) 

0.045 

(0.015) 

Lab2_SV 0.037 

(0.019) 

0.040 

(0.015) 

0.028 

(0.013) 

0.016 

(0.008) 

0.029 

(0.009) 

Lab3_WLI 0.003 

(0.003) 

0.000 

(0.000) 

0.004 

(0.002) 

0.010 

(0.008) 

0.002 

(0.002) 

Average 0.019 

(0.009) 

0.015 

(0.009) 

0.016 

(0.009) 

0.019 

(0.010) 

0.019 

(0.009) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

68 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Reproduction (Continued)

Abnormal Eggs as A 

Percentage of Eggs Laid 

Lab1_ST 0.055 

(0.022) 

0.024 

(0.008) 

0.069 

(0.043) 

0.036 

(0.014) 

0.017 

(0.005) 

Lab2_SV 0.056 

(0.028) 

0.046 

(0.015) 

0.041 

(0.016) 

0.020 

(0.009) 

0.062 

(0.020) 

Lab3_WLI 0.005 

(0.005) 

0.018 

(0.018) 

0.004 

(0.003) 

0.001 

(0.001) 

0.003 

(0.002) 

Average 0.023 

(0.010) 

0.026 

(0.011) 

0.020 

(0.009) 

0.016 

(0.009) 

0.018 

(0.009) 

Fertile Eggs as A 

Percentage of Eggs Set 

From Weeks 7 and 8 

Lab1_ST 0.938 

(0.022) 

0.972 

(0.013) 

0.898 

(0.066) 

0.943 

(0.028) 

0.958 

(0.017) 

Lab2_SV 0.979 

(0.012) 

0.960 

(0.026) 

0.952 

(0.027) 

0.944 

(0.036) 

0.970 

(0.022) 

Lab3_WLI 0.976 

(0.020) 

0.902 

(0.069) 

0.956 

(0.039) 

0.921 

(0.052) 

0.968 

(0.017) 

Average 0.971 

(0.009) 

0.967 

(0.012) 

0.948 

(0.021) 

0.940 

(0.020) 

0.965 

(0.011) 

Fertile Eggs as A 

Percentage of Eggs Set 

From Weeks 6 7 and 8 

Lab1_ST 0.929 

(0.022) 

0.878 

(0.063) 

0.865 

(0.069) 

0.937 

(0.024) 

0.935 

(0.023) 

Lab2_SV 0.986 

(0.008) 

0.971 

(0.017) 

0.956 

(0.024) 

0.949 

(0.033) 

0.978 

(0.017) 

Lab3_WLI 0.980 

(0.014) 

0.942 

(0.040) 

0.951 

(0.041) 

0.939 

(0.041) 

0.973 

(0.015) 

Average 0.969 

(0.015) 

0.949 

(0.021) 

0.937 

(0.024) 

0.947 

(0.022) 

0.963 

(0.016) 

Viable Eggs as A 

Percentage of Eggs Set 

From Weeks 7 and 8 

Lab1_ST 0.883 

(0.031) 

0.933 

(0.019) 

0.898 

(0.036) 

0.880 

(0.039) 

0.915 

(0.024) 

Lab2_SV 0.975 

(0.013) 

0.933 

(0.025) 

0.928 

(0.028) 

0.924 

(0.044) 

0.949 

(0.022) 

Lab3_WLI 0.957 

(0.021) 

0.888 

(0.068) 

0.888 

(0.054) 

0.865 

(0.062) 

0.881 

(0.049) 

Average 0.953 

(0.015) 

0.932 

(0.018) 

0.908 

(0.023) 

0.893 

(0.028) 

0.925 

(0.019) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

69 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Reproduction (Continued)

Viable Eggs as A 

Percentage of Eggs Set 

From Weeks 6 7 and 8 

Lab1_ST 0.847 

(0.034) 

0.891 

(0.033) 

0.880 

(0.037) 

0.883 

(0.039) 

0.882 

(0.031) 

Lab2_SV 0.977 

(0.010) 

0.939 

(0.020) 

0.933 

(0.024) 

0.921 

(0.041) 

0.952 

(0.026) 

Lab3_WLI 0.964 

(0.018) 

0.926 

(0.040) 

0.893 

(0.052) 

0.885 

(0.056) 

0.973 

(0.015) 

Average 0.947 

(0.025) 

0.918 

(0.028) 

0.908 

(0.030) 

0.903 

(0.034) 

0.945 

(0.026) 

Viable Eggs from Weeks 7 

and 8 after Two Weeks of 

Incubation as A 

Percentage of Viable Eggs 

From Weeks 7 and 8 

Lab1_ST 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Lab2_SV 0.991 

(0.006) 

1.000 

(0.000) 

0.965 

(0.020) 

0.969 

(0.011) 

0.995 

(0.005) 

Lab3_WLI 0.990 

(0.007) 

0.985 

(0.008) 

0.977 

(0.013) 

1.000 

(0.000) 

1.000 

(0.000) 

Average 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Viable Eggs from Weeks 6 

7 and 8 after Two Weeks 

of Incubation as A 

Percentage of Viable Eggs 

From Weeks 6 7 and 8 

Lab1_ST 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Lab2_SV 0.994 

(0.004) 

1.000 

(0.000) 

0.975 

(0.015) 

0.978 

(0.008) 

0.997 

(0.003) 

Lab3_WLI 0.990 

(0.006) 

0.981 

(0.008) 

0.984 

(0.009) 

1.000 

(0.000) 

1.000 

(0.000) 

Average 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Eggs Hatched as A 

Percentage of Number of 

Viable Eggs from Weeks 7 

and 8 after Two Weeks of 

Incubation 

Lab1_ST 0.834 

(0.035) 

0.935 

(0.022) 

0.913 

(0.034) 

0.884 

(0.029) 

0.935 

(0.036) 

Lab2_SV 0.942 

(0.020) 

0.905 

(0.043) 

0.874 

(0.060) 

0.895 

(0.054) 

0.946 

(0.021) 

Lab3_WLI 0.940 

(0.025) 

0.952 

(0.032) 

0.988 

(0.008) 

0.969 

(0.021) 

0.972 

(0.019) 

Average 0.917 

(0.024) 

0.941 

(0.025) 

0.954 

(0.023) 

0.926 

(0.025) 

0.949 

(0.023) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

70 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Reproduction (Continued)

Number of 14-Day-Old 

Surviving Chicks as A 

Percentage of Eggs 

Hatched 

Lab1_ST 0.993 

(0.007) 

0.974 

(0.012) 

0.864 

(0.038) 

0.944 

(0.033) 

0.989 

(0.008) 

Lab2_SV 0.896 

(0.016) 

0.978 

(0.011) 

0.967 

(0.013) 

0.988 

(0.012) 

0.968 

(0.015) 

Lab3_WLI 0.771 

(0.062) 

0.777 

(0.041) 

0.736 

(0.066) 

0.769 

(0.049) 

0.765 

(0.041) 

Average 0.886 

(0.070) 

0.911 

(0.069) 

0.867 

(0.071) 

0.906 

(0.070) 

0.911 

(0.069) 

Eggs Hatched as A 

Percentage of Number of 

Eggs Set from Weeks 7 

and 8 

Lab1_ST 0.739 

(0.042) 

0.873 

(0.030) 

0.824 

(0.049) 

0.772 

(0.038) 

0.853 

(0.037) 

Lab2_SV 0.909 

(0.020) 

0.840 

(0.044) 

0.821 

(0.061) 

0.817 

(0.064) 

0.892 

(0.028) 

Lab3_WLI 0.895 

(0.037) 

0.833 

(0.071) 

0.858 

(0.056) 

0.848 

(0.066) 

0.867 

(0.052) 

Average 0.862 

(0.028) 

0.859 

(0.033) 

0.835 

(0.038) 

0.805 

(0.037) 

0.874 

(0.030) 

Eggs Hatched as A 

Percentage of Number of 

Fertile Eggs from Weeks 7 

and 8 

Lab1_ST 0.785 

(0.039) 

0.898 

(0.027) 

0.850 

(0.041) 

0.824 

(0.037) 

0.891 

(0.036) 

Lab2_SV 0.929 

(0.021) 

0.879 

(0.041) 

0.839 

(0.061) 

0.842 

(0.056) 

0.921 

(0.021) 

Lab3_WLI 0.916 

(0.030) 

0.927 

(0.037) 

0.872 

(0.048) 

0.927 

(0.047) 

0.889 

(0.048) 

Average 0.892 

(0.024) 

0.906 

(0.026) 

0.858 

(0.033) 

0.864 

(0.031) 

0.906 

(0.025) 

Number of 14-Day-Old 

Surviving Chicks as A 

Percentage of Number of 

Eggs Set from Weeks 7 

and 8 

Lab1_ST 0.733 

(0.041) 

0.851 

(0.032) 

0.712 

(0.054) 

0.725 

(0.041) 

0.842 

(0.035) 

Lab2_SV 0.814 

(0.021) 

0.822 

(0.044) 

0.798 

(0.062) 

0.855 

(0.046) 

0.865 

(0.032) 

Lab3_WLI 0.711 

(0.067) 

0.659 

(0.069) 

0.662 

(0.061) 

0.650 

(0.064) 

0.655 

(0.050) 

Average 0.744 

(0.052) 

0.796 

(0.054) 

0.721 

(0.058) 

0.741 

(0.055) 

0.797 

(0.052) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

71 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Reproduction (Continued)

Eggs Hatched Per Hen Per 

Day 

Lab1_ST 0.554 

(0.048) 

0.719 

(0.042) 

0.602 

(0.060) 

0.638 

(0.034) 

0.647 

(0.050) 

Lab2_SV 0.778 

(0.034) 

0.696 

(0.056) 

0.702 

(0.055) 

0.707 

(0.059) 

0.738 

(0.048) 

Lab3_WLI 0.782 

(0.033) 

0.748 

(0.064) 

0.727 

(0.053) 

0.698 

(0.064) 

0.722 

(0.052) 

Average 0.718 

(0.041) 

0.730 

(0.046) 

0.678 

(0.047) 

0.685 

(0.045) 

0.703 

(0.045) 

Number of 14-Day-Old 

Surviving Chicks Per Hen 

Per Day 

Lab1_ST 0.549 

(0.047) 

0.700 

(0.043) 

0.510 

(0.053) 

0.603 

(0.039) 

0.639 

(0.050) 

Lab2_SV 0.692 

(0.029) 

0.680 

(0.055) 

0.724 

(0.037) 

0.740 

(0.045) 

0.715 

(0.050) 

Lab3_WLI 0.622 

(0.060) 

0.592 

(0.063) 

0.559 

(0.054) 

0.546 

(0.062) 

0.575 

(0.053) 

Average 0.615 

(0.048) 

0.670 

(0.051) 

0.609 

(0.050) 

0.635 

(0.050) 

0.644 

(0.051) 

Egg Shell and Weight

Pen Mean Egg Shell 

Thickness (mm) 

Lab1_ST 0.206 

(0.002) 

0.203 

(0.004) 

0.204 

(0.004) 

0.215 

(0.003) 

0.209 

(0.003) 

Lab2_SV 0.205 

(0.004) 

0.205 

(0.004) 

0.207 

(0.003) 

0.210 

(0.004) 

0.209 

(0.005) 

Lab3_WLI 0.234 

(0.004) 

0.231 

(0.004) 

0.229 

(0.003) 

0.234 

(0.004) 

0.225 

(0.002) 

Average 0.215 

(0.008) 

0.213 

(0.008) 

0.213 

(0.008) 

0.220 

(0.008) 

0.213 

(0.008) 

Pen Mean Egg Content 

Weight - Albumin (g) 

Lab1_ST 5.761 

(0.192) 

6.091 

(0.185) 

5.988 

(0.150) 

5.834 

(0.134) 

5.807 

(0.125) 

Lab2_SV 3.913 

(0.178) 

3.589 

(0.166) 

3.637 

(0.173) 

3.729 

(0.150) 

3.861 

(0.144) 

Lab3_WLI 4.565 

(0.112) 

4.401 

(0.153) 

4.430 

(0.148) 

4.809 

(0.094) 

4.564 

(0.102) 

Average 4.744 

(0.628) 

4.673 

(0.629) 

4.689 

(0.628) 

4.824 

(0.625) 

4.736 

(0.625) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

72 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Egg Shell and Weight (Continued)

Pen Mean Egg Content 

Weight - Yolk (g) 

Lab1_ST 3.660 

(0.136) 

3.787 

(0.146) 

3.779 

(0.160) 

3.593 

(0.078) 

3.616 

(0.107) 

Lab2_SV 4.065 

(0.171) 

4.140 

(0.112) 

4.149 

(0.153) 

4.341 

(0.113) 

3.850 

(0.144) 

Lab3_WLI 3.257 

(0.062) 

3.279 

(0.082) 

3.348 

(0.088) 

3.357 

(0.086) 

3.415 

(0.052) 

Average 3.646 

(0.239) 

3.712 

(0.239) 

3.744 

(0.242) 

3.742 

(0.236) 

3.684 

(0.237) 

Pen Mean Egg Content 

Weight - Combined (g) 

Lab1_ST 9.421 

(0.287) 

9.878 

(0.295) 

9.768 

(0.281) 

9.427 

(0.181) 

9.422 

(0.185) 

Lab2_SV 7.978 

(0.316) 

7.729 

(0.213) 

7.786 

(0.288) 

8.069 

(0.182) 

7.711 

(0.231) 

Lab3_WLI 7.823 

(0.148) 

7.679 

(0.204) 

7.778 

(0.121) 

8.165 

(0.154) 

7.980 

(0.108) 

Average 8.365 

(0.551) 

8.350 

(0.552) 

8.350 

(0.548) 

8.567 

(0.545) 

8.427 

(0.544) 

Thyroid Weight

Adult Left Thyroid Weight 

(mg) - Males 

Lab1_ST 6.113 

(0.830) 

6.672 

(0.778) 

7.125 

(1.017) 

7.238 

(0.804) 

8.029 

(0.851) 

Lab2_SV 11.800 

(1.407) 

7.581 

(0.996) 

8.140 

(0.915) 

9.463 

(1.093) 

12.189 

(0.908) 

Lab3_WLI 4.641 

(0.407) 

5.000 

(0.573) 

4.271 

(0.380) 

4.419 

(0.579) 

5.094 

(0.434) 

Average 7.089 

(1.537) 

6.655 

(1.533) 

6.533 

(1.528) 

7.011 

(1.539) 

8.178 

(1.524) 

Adult Left Thyroid Weight 

(mg) - Females 

Lab1_ST 10.138 

(0.897) 

9.500 

(0.996) 

10.473 

(1.809) 

12.688 

(1.739) 

9.029 

(1.610) 

Lab2_SV 11.362 

(1.150) 

13.925 

(1.682) 

12.900 

(1.542) 

13.671 

(1.442) 

15.712 

(2.854) 

Lab3_WLI 6.140 

(0.535) 

5.756 

(0.850) 

6.094 

(0.663) 

6.344 

(0.630) 

5.950 

(0.760) 

Average 9.495 

(2.004) 

9.397 

(2.048) 

9.718 

(2.047) 

10.238 

(2.039) 

9.516 

(2.077) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

73 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Bio Chem

Egg Yolk Estradiol (pg/g) 

Lab1_ST 17.934 

(2.934) 

27.555 

(5.623) 

30.087 

(5.188) 

28.998 

(4.149) 

29.763 

(4.936) 

Lab2_SV 47.766 

(4.137) 

25.508 

(4.735) 

28.615 

(8.578) 

44.578 

(3.635) 

39.612 

(5.175) 

Lab3_WLI 68.882 

(6.611) 

73.986 

(8.941) 

69.490 

(6.517) 

67.517 

(6.010) 

56.762 

(6.516) 

Average 44.119 

(12.664) 

40.465 

(12.909) 

43.806 

(12.970) 

47.218 

(12.668) 

42.546 

(12.791) 

Egg Yolk Testosterone 

(pg/g) 

Lab1_ST 2077.06 

(331.443) 

1254.92 

(245.148) 

2192.58 

(774.189) 

2122.04 

(480.240) 

2620.50 

(474.135) 

Lab2_SV 2685.20 

(698.976) 

4019.44 

(1028.19) 

2573.83 

(785.922) 

2209.49 

(357.412) 

3580.22 

(830.427) 

Lab3_WLI 1812.52 

(393.307) 

1848.09 

(432.038) 

2371.93 

(569.978) 

1827.49 

(419.938) 

1917.52 

(320.586) 

Average 2135.41 

(310.518) 

1653.72 

(301.007) 

2411.59 

(440.480) 

2028.82 

(303.960) 

2397.62 

(325.947) 

Plasma Thyroid Hormone 

T3 - Adult Males (ng/mL) 

Lab1_ST 0.497 

(0.080) 

0.404 

(0.032) 

0.512 

(0.034) 

0.882 

(0.169) 

0.595 

(0.063) 

Lab2_SV 1.016 

(0.171) 

0.833 

(0.076) 

1.266 

(0.136) 

0.658 

(0.066) 

0.765 

(0.047) 

Lab3_WLI 1.131 

(0.096) 

1.202 

(0.224) 

1.191 

(0.137) 

1.161 

(0.115) 

1.160 

(0.108) 

Average 0.862 

(0.204) 

0.778 

(0.203) 

0.955 

(0.202) 

0.845 

(0.204) 

0.834 

(0.198) 

Plasma Thyroid Hormone 

T3 - Adult Females 

(ng/mL) 

Lab1_ST 0.658 

(0.088) 

0.782 

(0.158) 

0.460 

(0.064) 

0.598 

(0.076) 

0.650 

(0.080) 

Lab2_SV 1.030 

(0.059) 

0.800 

(0.158) 

0.753 

(0.134) 

0.819 

(0.166) 

0.639 

(0.029) 

Lab3_WLI 0.869 

(0.094) 

1.113 

(0.129) 

0.816 

(0.101) 

0.791 

(0.074) 

0.870 

(0.099) 

Average 0.872 

(0.096) 

0.911 

(0.120) 

0.656 

(0.101) 

0.722 

(0.102) 

0.695 

(0.094) 

A decreasing trend 

for the highest 

concentration 

group 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

74 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Bio Chem(Continued)

Plasma Thyroid Hormone 

T4 - Adult Males (ng/mL) 

Lab1_ST 3.764 

(1.235) 

5.202 

(1.244) 

10.035 

(2.213) 

1.528 

(0.469) 

6.236 

(0.881) 

Lab2_SV 3.178 

(0.675) 

6.699 

(2.655) 

7.479 

(2.647) 

6.300 

(1.521) 

1.736 

(0.972) 

Lab3_WLI 3.109 

(0.364) 

3.686 

(0.571) 

3.391 

(0.333) 

1.657 

(0.322) 

2.329 

(0.818) 

Average 3.367 

(1.178) 

4.827 

(1.344) 

5.876 

(1.423) 

2.698 

(1.184) 

3.458 

(1.202) 

Plasma Thyroid Hormone 

T4 - Adult Females 

(ng/mL) 

Lab1_ST 1.910 

(0.548) 

6.584 

(2.193) 

4.904 

(1.183) 

2.709 

(0.767) 

4.014 

(1.347) 

Lab2_SV 3.328 

(1.066) 

4.029 

(2.084) 

5.860 

(1.724) 

3.347 

(0.739) 

2.903 

(0.577) 

Lab3_WLI 1.138 

(0.237) 

3.744 

(1.891) 

1.011 

(0.247) 

0.951 

(0.188) 

1.176 

(0.413) 

Average 2.125 

(0.786) 

4.761 

(1.384) 

2.728 

(0.835) 

2.284 

(0.782) 

2.429 

(0.814) 

Plasma Sex Hormone 

Estradiol - Adult Males 

(pg/mL) 

Lab1_ST 4.988 

(0.531) 

3.017 

(0.631) 

3.518 

(0.461) 

2.245 

(0.435) 

3.574 

(0.761) 

Lab2_SV 3.796 

(0.605) 

4.267 

(0.781) 

8.597 

(2.143) 

3.295 

(0.378) 

3.793 

(0.813) 

Lab3_WLI 6.042 

(1.501) 

4.789 

(1.089) 

5.129 

(1.429) 

4.763 

(1.063) 

3.700 

(0.000) 

Average 4.696 

(0.551) 

3.870 

(0.582) 

4.307 

(0.643) 

3.117 

(0.476) 

3.574 

(0.459) 

Plasma Sex Hormone 

Estradiol - Adult Females 

(pg/mL) 

Lab1_ST 12.022 

(1.721) 

11.564 

(2.567) 

11.192 

(1.118) 

16.401 

(6.849) 

14.881 

(1.881) 

Lab2_SV 18.032 

(3.786) 

19.829 

(3.172) 

14.759 

(3.100) 

15.458 

(3.013) 

16.190 

(1.949) 

Lab3_WLI 25.273 

(1.824) 

23.267 

(3.785) 

41.560 

(4.172) 

24.490 

(6.174) 

18.445 

(3.907) 

Average 18.306 

(4.850) 

18.234 

(4.983) 

20.986 

(4.920) 

18.226 

(5.489) 

17.101 

(4.871) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

75 

Table 14. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F0 Generationf

 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Significant 

Concentration 

Effectd,e Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Bio Chem (Continued)

Plasma Sex Hormone 

Testosterone - Adult 

Males (pg/mL) 

Lab1_ST 1242.50 

(317.745) 

2340.42 

(1121.13) 

2097.28 

(717.514) 

1488.41 

(685.518) 

1887.61 

(1161.72) 

Lab2_SV 1151.09 

(184.708) 

2715.29 

(938.163) 

1820.51 

(883.029) 

1512.84 

(376.464) 

1683.99 

(440.844) 

Lab3_WLI 2942.39 

(518.354) 

3124.31 

(696.018) 

3266.85 

(963.748) 

3455.77 

(1885.67) 

4156.95 

(505.914) 

Average 1751.80 

(580.835) 

2565.78 

(747.736) 

2429.24 

(733.765) 

2084.97 

(651.233) 

2612.47 

(641.765) 

Plasma Sex Hormone 

Testosterone - Adult 

Females (pg/mL) 

Lab1_ST 327.461 

(63.129) 

542.563 

(168.052) 

87.385 

(14.683) 

229.229 

(147.465) 

594.631 

(218.672) 

Lab2_SV 317.664 

(185.839) 

478.192 

(77.943) 

73.609 

(21.750) 

324.969 

(96.703) 

265.242 

(128.318) 

Lab3_WLI 606.982 

(293.482) 

573.873 

(184.964) 

1286.95 

(279.762) 

595.393 

(316.128) 

525.616 

(331.143) 

Average 337.621 

(58.572) 

500.335 

(66.046) 

85.346 

(12.158) 

314.555 

(78.343) 

367.295 

(104.964) Yes 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

76 

Table 15. Mean and Standard Errors and Comparisons of Avian Vinclozolin Concentrations to 

Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Body Weight

Adult Body Weight (g) 

Day 0 - Males 

Lab1_ST 165.311 

(1.914) 

171.300 

(3.439) 

162.367 

(2.235) 

161.639 

(2.530) 

176.533 

(3.118) 

Lab2_SV 155.483 

(3.072) 

157.772 

(2.491) 

159.933 

(2.465) 

161.000 

(2.108) 

155.354 

(2.979) 

Lab3_WLI 94.063 

(2.950) 

99.200 

(2.375) 

87.765 

(3.061) 

90.824 

(4.031) 

88.833 

(3.107) 

Average 138.237 

(23.735) 

142.741 

(23.739) 

136.742 

(23.732) 

137.980 

(23.743) 

140.198 

(23.751) 

Adult Body Weight (g) 

Day 56 - Males 

Lab1_ST 213.494 

(4.988) 

212.100 

(6.693) 

205.522 

(5.603) 

208.213 

(4.286) 

208.835 

(4.649) 

Lab2_SV 216.300 

(3.746) 

231.678 

(3.588) 

225.556 

(4.680) 

228.206 

(4.736) 

222.067 

(4.758) 

Lab3_WLI 119.133 

(3.027) 

120.733 

(3.841) 

119.875 

(1.925) 

118.412 

(3.127) 

120.000 

(1.728) 

Average 182.342 

(32.711) 

188.680 

(32.735) 

184.363 

(32.695) 

184.331 

(32.717) 

184.355 

(32.687) 

Adult Body Weight (g) 

Day 0 - Females 

Lab1_ST 184.256 

(4.289) 

181.289 

(3.634) 

171.406 

(3.899) 

164.861 

(2.242) 

176.306 

(3.375) 

Lab2_SV 168.706 

(3.977) 

168.717 

(2.228) 

171.450 

(3.497) 

173.228 

(2.795) 

172.961 

(3.017) 

Lab3_WLI 96.875 

(3.657) 

94.333 

(3.266) 

92.706 

(2.839) 

96.529 

(4.823) 

97.500 

(3.252) 

Average 149.750 

(26.095) 

147.703 

(26.053) 

145.187 

(26.068) 

144.064 

(26.061) 

148.928 

(26.060) 

Adult Body Weight (g) 

Day 56 - Females 

Lab1_ST 267.650 

(6.162) 

271.063 

(5.602) 

258.422 

(5.386) 

262.194 

(4.650) 

259.429 

(7.210) 

Lab2_SV 266.482 

(7.608) 

274.182 

(5.622) 

280.128 

(5.377) 

279.867 

(5.831) 

280.994 

(5.957) 

Lab3_WLI 153.313 

(2.650) 

152.067 

(2.285) 

146.250 

(2.877) 

156.176 

(3.508) 

154.056 

(2.272) 

Average 231.461 

(39.720) 

231.487 

(39.701) 

226.828 

(39.713) 

233.160 

(39.723) 

232.632 

(39.706) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

77 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Body Weight (Continued) 

Mean Offspring Body 

Weight by Pen (g) Day 0 

Lab1_ST 8.756 

(0.191) 

9.213 

(0.211) 

9.036 

(0.210) 

8.819 

(0.167) 

8.841 

(0.183) 

Lab2_SV 9.571 

(0.173) 

9.444 

(0.153) 

9.575 

(0.195) 

9.600 

(0.139) 

9.388 

(0.176) 

Lab3_WLI 6.776 

(0.147) 

6.975 

(0.104) 

6.778 

(0.146) 

7.061 

(0.096) 

6.922 

(0.145) 

Average 8.364 

(0.788) 

8.503 

(0.786) 

8.424 

(0.789) 

8.531 

(0.786) 

8.392 

(0.788) 

Mean Offspring Body 

Weight by Pen (g) Day 14 

Lab1_ST 82.688 

(0.869) 

85.233 

(1.033) 

82.571 

(1.729) 

80.275 

(0.724) 

80.924 

(1.234) 

Lab2_SV 78.929 

(1.611) 

76.150 

(1.258) 

82.069 

(1.278) 

81.476 

(0.863) 

83.088 

(0.856) 

Lab3_WLI 48.529 

(0.908) 

47.375 

(1.004) 

48.706 

(0.731) 

49.111 

(1.120) 

48.111 

(1.081) 

Average 70.161 

(11.104) 

69.689 

(11.103) 

71.112 

(11.109) 

70.233 

(11.097) 

70.807 

(11.102) 

Feed Consumption

Adult Feed Consumption 

(g/bird/day) - Week 1

Lab1_ST 31.278 

(0.587) 

32.222 

(0.838) 

30.222 

(0.765) 

28.667 

(0.616) 

30.222 

(0.608) 

Lab2_SV 30.228 

(0.551) 

32.061 

(0.951) 

33.456 

(1.316) 

32.233 

(1.159) 

31.078 

(0.708) 

Lab3_WLI 20.250 

(0.704) 

21.533 

(0.559) 

22.412 

(0.845) 

21.471 

(0.723) 

20.833 

(0.513) 

Average 27.264 

(3.317) 

28.546 

(3.328) 

28.494 

(3.343) 

27.231 

(3.331) 

27.377 

(3.317) 

Adult Feed Consumption 

(g/bird/day) - Week 2 

Lab1_ST 31.444 

(0.622) 

30.389 

(1.545) 

31.222 

(0.756) 

33.889 

(0.620) 

30.667 

(1.245) 

Lab2_SV 36.561 

(0.873) 

38.711 

(0.983) 

42.278 

(1.109) 

39.256 

(1.187) 

37.378 

(1.108) 

Lab3_WLI 21.250 

(0.413) 

20.200 

(0.685) 

21.438 

(0.447) 

21.176 

(0.666) 

21.889 

(0.504) 

Average 29.863 

(5.152) 

29.801 

(5.173) 

31.381 

(5.158) 

31.502 

(5.160) 

30.182 

(5.167) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

78 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Feed Consumption (Continued) 

Adult Feed Consumption 

(g/bird/day) - Week 3 

Lab1_ST 32.889 

(0.911) 

30.353 

(1.257) 

31.222 

(0.603) 

30.611 

(0.776) 

30.111 

(2.010) 

Lab2_SV 31.500 

(1.156) 

33.511 

(0.560) 

33.822 

(0.756) 

34.450 

(0.785) 

33.150 

(1.001) 

Lab3_WLI 21.750 

(0.413) 

21.067 

(0.727) 

21.250 

(0.512) 

21.294 

(0.561) 

22.167 

(0.567) 

Average 29.026 

(3.733) 

28.492 

(3.738) 

28.707 

(3.729) 

28.733 

(3.734) 

29.095 

(3.746) 

Adult Feed Consumption 

(g/bird/day) - Week 4 

Lab1_ST 32.333 

(0.904) 

30.059 

(1.202) 

28.556 

(0.612) 

29.556 

(0.785) 

29.529 

(0.924) 

Lab2_SV 25.844 

(0.949) 

26.483 

(0.920) 

27.100 

(1.005) 

29.239 

(0.783) 

28.444 

(1.078) 

Lab3_WLI 22.500 

(0.387) 

22.267 

(0.483) 

22.125 

(0.562) 

22.294 

(0.574) 

23.167 

(0.584) 

Average 26.856 

(2.274) 

26.239 

(2.286) 

25.888 

(2.272) 

26.978 

(2.271) 

27.047 

(2.287) 

Adult Feed Consumption 

(g/bird/day) - Week 5 

Lab1_ST 30.222 

(0.794) 

31.125 

(1.048) 

29.278 

(0.671) 

29.056 

(0.439) 

28.647 

(0.757) 

Lab2_SV 26.678 

(1.010) 

30.000 

(0.697) 

27.817 

(0.783) 

32.711 

(0.539) 

30.061 

(0.709) 

Lab3_WLI 22.438 

(0.695) 

22.867 

(0.646) 

22.750 

(0.574) 

22.706 

(0.635) 

24.111 

(0.704) 

Average 26.534 

(2.364) 

27.919 

(2.360) 

26.641 

(2.348) 

28.158 

(2.336) 

27.617 

(2.352) 

Adult Feed Consumption 

(g/bird/day) - Week 6 

Lab1_ST 32.611 

(1.039) 

32.625 

(1.143) 

30.833 

(0.622) 

31.222 

(0.952) 

30.412 

(0.970) 

Lab2_SV 27.444 

(0.902) 

31.411 

(0.629) 

29.367 

(1.093) 

33.950 

(0.550) 

32.789 

(0.769) 

Lab3_WLI 22.467 

(0.506) 

21.867 

(0.798) 

22.438 

(0.316) 

21.765 

(0.774) 

23.000 

(0.667) 

Average 27.510 

(3.130) 

28.584 

(3.133) 

27.639 

(3.121) 

29.081 

(3.125) 

28.783 

(3.128) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

79 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Feed Consumption (Continued) 

Adult Feed Consumption 

(g/bird/day) - Week 7 

Lab1_ST 32.667 

(0.878) 

31.875 

(0.779) 

30.278 

(0.847) 

29.000 

(0.700) 

29.353 

(0.931) 

Lab2_SV 30.022 

(0.685) 

32.356 

(1.456) 

32.433 

(1.465) 

38.194 

(1.039) 

36.150 

(0.997) 

Lab3_WLI 22.600 

(0.388) 

23.000 

(0.586) 

21.500 

(0.398) 

21.588 

(0.670) 

22.778 

(0.527) 

Average 28.398 

(3.654) 

29.186 

(3.677) 

28.089 

(3.675) 

29.469 

(3.663) 

29.406 

(3.665) 

Adult Feed Consumption 

(g/bird/day) - Week 8 

Lab1_ST 32.556 

(1.500) 

33.125 

(1.463) 

31.222 

(0.989) 

31.500 

(0.974) 

30.706 

(1.067) 

Lab2_SV 29.117 

(0.549) 

30.911 

(0.602) 

30.433 

(0.611) 

33.000 

(0.506) 

31.411 

(0.827) 

Lab3_WLI 21.600 

(0.505) 

22.200 

(0.745) 

21.438 

(0.645) 

20.882 

(0.831) 

22.500 

(0.595) 

Average 27.399 

(3.232) 

28.511 

(3.241) 

27.686 

(3.228) 

28.692 

(3.230) 

28.330 

(3.235) 

Adult Feed Consumption 

(g/bird/day) - Week 9 

Lab1_ST 31.944 

(1.259) 

31.250 

(1.063) 

31.444 

(0.653) 

32.167 

(0.841) 

30.824 

(0.682) 

Lab2_SV 31.267 

(0.622) 

33.967 

(0.528) 

32.678 

(0.870) 

36.222 

(0.973) 

33.261 

(0.647) 

Lab3_WLI NA NA NA NA NA 

Average 31.113 

(1.246) 

32.770 

(1.205) 

32.103 

(1.203) 

34.094 

(1.253) 

32.050 

(1.176) 

Adult Feed Consumption 

(g/bird/day) - Week 10 

Lab1_ST NA NA NA NA NA 

Lab2_SV 30.978 

(0.536) 

34.789 

(1.128) 

34.172 

(1.078) 

35.639 

(1.022) 

34.567 

(1.173) 

Lab3_WLI NA NA NA NA NA 

Average NA NA NA NA NA 

Adult Feed Consumption 

(g/bird/day) - Week 11 

Lab1_ST NA NA NA NA NA 

Lab2_SV 26.756 

(1.416) 

29.428 

(1.365) 

29.367 

(1.272) 

30.406 

(1.798) 

30.411 

(1.379) 

Lab3_WLI NA NA NA NA NA 

Average NA NA NA NA NA 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

80 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction

Eggs Laid 

Lab1_ST 46.222 

(1.086) 

44.250 

(1.743) 

45.444 

(1.663) 

44.875 

(1.390) 

45.625 

(1.429) 

Lab2_SV 39.444 

(2.211) 

42.389 

(1.708) 

37.611 

(2.640) 

43.000 

(1.755) 

42.889 

(1.252) 

Lab3_WLI 43.688 

(1.036) 

39.800 

(2.864) 

40.938 

(2.378) 

42.059 

(1.686) 

45.222 

(0.920) 

Average 43.854 

(1.249) 

42.732 

(1.515) 

42.087 

(1.584) 

43.262 

(1.364) 

44.824 

(1.212) 

Eggs Crack 

Lab1_ST 0.889 

(0.342) 

0.938 

(0.433) 

1.222 

(0.454) 

1.375 

(0.491) 

0.875 

(0.352) 

Lab2_SV 1.833 

(0.480) 

1.833 

(0.550) 

1.778 

(0.619) 

2.333 

(1.007) 

2.056 

(0.439) 

Lab3_WLI 0.563 

(0.302) 

0.333 

(0.126) 

0.125 

(0.085) 

0.059 

(0.059) 

0.111 

(0.076) 

Average 1.139 

(0.528) 

1.129 

(0.508) 

0.952 

(0.503) 

0.885 

(0.501) 

0.935 

(0.501) 

Eggs Abnormal 

Lab1_ST 0.722 

(0.226) 

0.563 

(0.203) 

0.500 

(0.202) 

0.750 

(0.281) 

2.250 

(1.109) 

Lab2_SV 3.389 

(0.879) 

2.944 

(0.920) 

3.556 

(1.216) 

3.556 

(1.269) 

3.167 

(0.653) 

Lab3_WLI 0.375 

(0.155) 

0.267 

(0.153) 

0.313 

(0.151) 

0.059 

(0.059) 

0.389 

(0.244) 

Average 1.431 

(0.907) 

1.296 

(0.907) 

1.310 

(0.907) 

1.136 

(0.902) 

1.509 

(0.922) 

Eggs Set From Weeks 7 

and 8 

Lab1_ST 10.944 

(0.563) 

11.188 

(0.510) 

11.056 

(0.688) 

11.375 

(0.455) 

10.813 

(0.726) 

Lab2_SV 10.611 

(0.696) 

11.235 

(0.315) 

11.294 

(0.580) 

11.056 

(0.482) 

11.000 

(0.618) 

Lab3_WLI 12.438 

(0.316) 

11.733 

(0.802) 

11.188 

(0.833) 

12.118 

(0.410) 

12.056 

(0.400) 

Average 11.600 

(0.414) 

11.477 

(0.413) 

11.291 

(0.503) 

11.524 

(0.407) 

11.366 

(0.443) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

81 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction(Continued) 

Eggs Set From Weeks 6 7 

and 8 

Lab1_ST 16.722 

(0.646) 

17.000 

(0.707) 

16.500 

(1.061) 

16.938 

(0.642) 

16.000 

(1.190) 

Lab2_SV 16.167 

(1.058) 

16.824 

(0.487) 

16.471 

(0.823) 

16.222 

(0.794) 

16.333 

(0.967) 

Lab3_WLI 39.188 

(1.188) 

35.867 

(2.724) 

37.063 

(2.285) 

38.706 

(1.578) 

40.833 

(0.908) 

Average 24.080 

(7.618) 

24.448 

(7.614) 

23.946 

(7.628) 

24.154 

(7.617) 

24.564 

(7.622) 

Eggs Fertile From Weeks 

7 and 8 

Lab1_ST 10.278 

(0.722) 

9.938 

(0.733) 

10.667 

(0.691) 

9.688 

(0.845) 

9.625 

(0.851) 

Lab2_SV 10.389 

(0.701) 

10.941 

(0.337) 

11.000 

(0.549) 

10.444 

(0.584) 

10.941 

(0.609) 

Lab3_WLI 12.063 

(0.622) 

11.533 

(0.792) 

11.063 

(0.844) 

11.588 

(0.613) 

12.000 

(0.420) 

Average 10.941 

(0.598) 

10.930 

(0.548) 

11.004 

(0.594) 

10.608 

(0.591) 

11.043 

(0.561) 

Eggs Fertile From Weeks 

6 7 and 8 

Lab1_ST 15.667 

(0.836) 

15.375 

(0.921) 

15.889 

(1.084) 

14.125 

(1.255) 

14.188 

(1.262) 

Lab2_SV 15.611 

(1.094) 

16.471 

(0.486) 

15.941 

(0.816) 

15.111 

(0.996) 

16.167 

(0.954) 

Lab3_WLI 37.875 

(1.408) 

34.867 

(2.777) 

35.438 

(2.497) 

37.118 

(1.894) 

38.667 

(1.319) 

Average 23.096 

(7.345) 

23.239 

(7.337) 

22.971 

(7.350) 

22.026 

(7.356) 

22.996 

(7.349) 

Eggs Viable From Weeks 

7 and 8 

Lab1_ST 10.222 

(0.721) 

9.438 

(0.730) 

10.667 

(0.691) 

9.188 

(0.927) 

9.313 

(0.934) 

Lab2_SV 10.167 

(0.742) 

10.765 

(0.359) 

10.765 

(0.532) 

10.056 

(0.712) 

10.706 

(0.652) 

Lab3_WLI 17.250 

(0.854) 

16.867 

(1.041) 

15.938 

(1.199) 

16.529 

(1.105) 

16.889 

(0.536) 

Average 12.522 

(2.249) 

12.475 

(2.231) 

12.684 

(2.243) 

11.895 

(2.262) 

12.414 

(2.239) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

82 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction (Continued) 

Eggs Viable From Weeks 

6 7 and 8 

Lab1_ST 15.333 

(0.856) 

14.563 

(0.992) 

16.706 

(0.611) 

13.563 

(1.332) 

13.688 

(1.405) 

Lab2_SV 15.278 

(1.157) 

16.294 

(0.498) 

15.529 

(0.800) 

14.611 

(1.112) 

15.833 

(0.968) 

Lab3_WLI 36.375 

(1.502) 

32.800 

(2.797) 

34.125 

(2.553) 

34.412 

(2.853) 

36.500 

(1.543) 

Average 22.244 

(6.734) 

22.207 

(6.730) 

22.814 

(6.729) 

20.829 

(6.759) 

22.014 

(6.742) 

Viable Eggs From Weeks 

7 and 8 after Two Weeks 

of Incubation 

Lab1_ST 10.222 

(0.721) 

9.438 

(0.730) 

10.667 

(0.691) 

9.188 

(0.927) 

9.313 

(0.934) 

Lab2_SV 10.056 

(0.747) 

10.059 

(0.511) 

10.235 

(0.566) 

9.944 

(0.702) 

10.706 

(0.652) 

Lab3_WLI 16.938 

(0.910) 

16.667 

(1.094) 

15.750 

(1.233) 

16.412 

(1.075) 

16.722 

(0.565) 

Average 12.382 

(2.212) 

12.002 

(2.203) 

12.407 

(2.207) 

11.846 

(2.222) 

12.378 

(2.201) 

Viable Eggs From Weeks 

6 7 and 8 after Two Weeks 

of Incubation 

Lab1_ST 15.333 

(0.856) 

14.563 

(0.992) 

15.778 

(1.092) 

13.563 

(1.332) 

13.688 

(1.405) 

Lab2_SV 15.167 

(1.153) 

15.588 

(0.613) 

15.000 

(0.767) 

14.500 

(1.100) 

15.833 

(0.968) 

Lab3_WLI 16.938 

(0.910) 

16.667 

(1.094) 

15.750 

(1.233) 

16.353 

(1.095) 

16.722 

(0.565) 

Average 15.877 

(0.723) 

15.665 

(0.674) 

15.459 

(0.735) 

14.886 

(0.819) 

15.776 

(0.677) 

Eggs Hatched 

Lab1_ST 9.333 

(0.780) 

8.438 

(0.837) 

10.000 

(0.705) 

8.500 

(0.940) 

8.438 

(1.053) 

Lab2_SV 9.389 

(0.837) 

9.176 

(0.649) 

9.059 

(0.672) 

9.333 

(0.754) 

10.059 

(0.672) 

Lab3_WLI 11.438 

(0.707) 

10.733 

(0.790) 

10.688 

(0.820) 

10.824 

(0.734) 

11.056 

(0.569) 

Average 10.093 

(0.700) 

9.474 

(0.692) 

9.920 

(0.685) 

9.612 

(0.711) 

10.042 

(0.679) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

83 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction (Continued) 

Number of 14-Day-Old 

Surviving Chicks 

Lab1_ST 8.944 

(0.769) 

8.313 

(0.835) 

10.000 

(0.705) 

8.188 

(0.905) 

8.733 

(0.968) 

Lab2_SV 9.111 

(0.808) 

8.824 

(0.666) 

8.529 

(0.665) 

9.056 

(0.721) 

9.706 

(0.674) 

Lab3_WLI 10.875 

(0.724) 

10.600 

(0.767) 

9.938 

(0.772) 

10.235 

(0.725) 

10.667 

(0.657) 

Average 9.665 

(0.631) 

9.266 

(0.624) 

9.486 

(0.610) 

9.233 

(0.635) 

9.812 

(0.621) 

Eggs Laid Per Hen Per 

Day 

Lab1_ST 0.943 

(0.022) 

0.903 

(0.036) 

0.928 

(0.034) 

0.916 

(0.028) 

0.931 

(0.029) 

Lab2_SV 0.807 

(0.045) 

0.879 

(0.025) 

0.828 

(0.075) 

0.879 

(0.036) 

0.888 

(0.025) 

Lab3_WLI 0.892 

(0.021) 

0.812 

(0.058) 

0.836 

(0.049) 

0.858 

(0.034) 

0.923 

(0.019) 

Average 0.899 

(0.022) 

0.883 

(0.025) 

0.878 

(0.031) 

0.885 

(0.024) 

0.919 

(0.021) 

Cracked Eggs as A 

Percentage of Eggs Laid 

Lab1_ST 0.020 

(0.008) 

0.021 

(0.009) 

0.026 

(0.009) 

0.031 

(0.012) 

0.021 

(0.008) 

Lab2_SV 0.049 

(0.014) 

0.042 

(0.013) 

0.039 

(0.010) 

0.055 

(0.023) 

0.047 

(0.010) 

Lab3_WLI 0.014 

(0.008) 

0.017 

(0.010) 

0.004 

(0.002) 

0.001 

(0.001) 

0.002 

(0.002) 

Average 0.027 

(0.012) 

0.027 

(0.013) 

0.022 

(0.012) 

0.020 

(0.012) 

0.021 

(0.012) 

Abnormal Eggs as A 

Percentage of Eggs Laid 

Lab1_ST 0.017 

(0.005) 

0.014 

(0.006) 

0.012 

(0.005) 

0.018 

(0.007) 

0.054 

(0.028) 

Lab2_SV 0.092 

(0.026) 

0.069 

(0.024) 

0.105 

(0.054) 

0.085 

(0.029) 

0.073 

(0.016) 

Lab3_WLI 0.009 

(0.004) 

0.006 

(0.003) 

0.007 

(0.003) 

0.001 

(0.001) 

0.009 

(0.005) 

Average 0.034 

(0.022) 

0.031 

(0.022) 

0.031 

(0.022) 

0.027 

(0.022) 

0.035 

(0.022) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

84 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction (Continued) 

Fertile Eggs as A 

Percentage of Eggs Set 

From Weeks 7 and 8 

Lab1_ST 0.925 

(0.040) 

0.876 

(0.043) 

0.964 

(0.015) 

0.839 

(0.060) 

0.889 

(0.045) 

Lab2_SV 0.979 

(0.009) 

0.974 

(0.012) 

0.977 

(0.009) 

0.945 

(0.032) 

0.995 

(0.005) 

Lab3_WLI 0.956 

(0.039) 

0.984 

(0.008) 

0.988 

(0.012) 

0.943 

(0.036) 

0.994 

(0.006) 

Average 0.967 

(0.015) 

0.968 

(0.014) 

0.973 

(0.013) 

0.924 

(0.025) 

0.984 

(0.012) Yes 

Fertile Eggs as A 

Percentage of Eggs Set 

From Weeks 6 7 and 8 

Lab1_ST 0.932 

(0.028) 

0.896 

(0.029) 

0.960 

(0.015) 

0.823 

(0.061) 

0.894 

(0.041) 

Lab2_SV 0.965 

(0.017) 

0.980 

(0.010) 

0.968 

(0.012) 

0.932 

(0.038) 

0.991 

(0.005) 

Lab3_WLI 0.963 

(0.017) 

0.971 

(0.017) 

0.941 

(0.022) 

0.956 

(0.024) 

0.943 

(0.018) 

Average 0.954 

(0.019) 

0.958 

(0.018) 

0.958 

(0.017) 

0.930 

(0.025) 

0.959 

(0.018) 

Viable Eggs as A 

Percentage of Eggs Set 

From Weeks 7 and 8 

Lab1_ST 0.920 

(0.039) 

0.832 

(0.043) 

0.964 

(0.015) 

0.792 

(0.066) 

0.832 

(0.055) 

Lab2_SV 0.956 

(0.021) 

0.958 

(0.015) 

0.957 

(0.012) 

0.912 

(0.047) 

0.972 

(0.016) 

Lab3_WLI 0.935 

(0.033) 

0.949 

(0.026) 

0.903 

(0.061) 

0.892 

(0.059) 

0.934 

(0.018) 

Average 0.938 

(0.023) 

0.931 

(0.021) 

0.956 

(0.020) 

0.874 

(0.036) 

0.937 

(0.021) 

Viable Eggs as A 

Percentage of Eggs Set 

From Weeks 6 7 and 8 

Lab1_ST 0.913 

(0.031) 

0.846 

(0.036) 

0.954 

(0.018) 

0.788 

(0.065) 

0.833 

(0.055) 

Lab2_SV 0.943 

(0.029) 

0.969 

(0.011) 

0.944 

(0.014) 

0.904 

(0.048) 

0.971 

(0.012) 

Lab3_WLI 0.924 

(0.021) 

0.914 

(0.033) 

0.900 

(0.031) 

0.875 

(0.061) 

0.889 

(0.026) 

Average 0.927 

(0.027) 

0.921 

(0.027) 

0.935 

(0.025) 

0.860 

(0.040) 

0.918 

(0.027) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

85 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction (Continued) 

Viable Eggs from Weeks 7 

and 8 after Two Weeks of 

Incubation as A 

Percentage of Viable Eggs 

From Weeks 7 and 8 

Lab1_ST 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Lab2_SV 0.989 

(0.008) 

0.932 

(0.031) 

0.955 

(0.026) 

0.990 

(0.007) 

1.000 

(0.000) 

Lab3_WLI 0.981 

(0.016) 

0.979 

(0.014) 

0.984 

(0.008) 

0.990 

(0.007) 

0.989 

(0.006) 

Average 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Viable Eggs from Weeks 6 

7 and 8 after Two Weeks 

of Incubation as A 

Percentage of Viable Eggs 

From Weeks 6 7 and 8 

Lab1_ST 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Lab2_SV 0.993 

(0.005) 

0.956 

(0.020) 

0.970 

(0.017) 

0.993 

(0.005) 

1.000 

(0.000) 

Lab3_WLI 0.981 

(0.016) 

0.979 

(0.014) 

0.984 

(0.008) 

0.990 

(0.007) 

0.989 

(0.006) 

Average 1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

1.000 

(0.000) 

Eggs Hatched as A 

Percentage of Number of 

Viable Eggs from Weeks 7 

and 8 after Two Weeks of 

Incubation 

Lab1_ST 0.905 

(0.033) 

0.877 

(0.046) 

0.935 

(0.020) 

0.909 

(0.028) 

0.814 

(0.076) 

Lab2_SV 0.917 

(0.040) 

0.904 

(0.037) 

0.874 

(0.036) 

0.932 

(0.025) 

0.929 

(0.023) 

Lab3_WLI 0.983 

(0.009) 

0.979 

(0.009) 

0.989 

(0.007) 

0.968 

(0.013) 

0.975 

(0.012) 

Average 0.941 

(0.024) 

0.936 

(0.024) 

0.948 

(0.023) 

0.932 

(0.024) 

0.935 

(0.024) 

Number of 14-Day-Old 

Surviving Chicks as A 

Percentage of Eggs 

Hatched 

Lab1_ST 0.956 

(0.019) 

0.986 

(0.010) 

1.000 

(0.000) 

0.963 

(0.019) 

0.970 

(0.022) 

Lab2_SV 0.976 

(0.012) 

0.960 

(0.016) 

0.937 

(0.017) 

0.975 

(0.010) 

0.966 

(0.014) 

Lab3_WLI 0.949 

(0.017) 

0.990 

(0.007) 

0.933 

(0.022) 

0.950 

(0.023) 

0.957 

(0.022) 

Average 0.962 

(0.014) 

0.981 

(0.012) 

0.969 

(0.013) 

0.966 

(0.014) 

0.965 

(0.015) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

86 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction (Continued) 

Eggs Hatched as A 

Percentage of Number of 

Eggs Set from Weeks 7 

and 8 

Lab1_ST 0.830 

(0.047) 

0.739 

(0.058) 

0.903 

(0.027) 

0.734 

(0.070) 

0.724 

(0.083) 

Lab2_SV 0.869 

(0.045) 

0.811 

(0.046) 

0.793 

(0.037) 

0.842 

(0.051) 

0.903 

(0.028) 

Lab3_WLI 0.904 

(0.050) 

0.920 

(0.027) 

0.956 

(0.019) 

0.872 

(0.054) 

0.908 

(0.029) 

Average 0.868 

(0.045) 

0.836 

(0.043) 

0.891 

(0.039) 

0.822 

(0.049) 

0.872 

(0.043) 

Eggs Hatched as A 

Percentage of Number of 

Fertile Eggs from Weeks 7 

and 8 

Lab1_ST 0.901 

(0.034) 

0.835 

(0.048) 

0.935 

(0.020) 

0.854 

(0.042) 

0.802 

(0.079) 

Lab2_SV 0.888 

(0.045) 

0.827 

(0.043) 

0.812 

(0.036) 

0.872 

(0.041) 

0.906 

(0.027) 

Lab3_WLI 0.936 

(0.030) 

0.934 

(0.024) 

0.967 

(0.011) 

0.903 

(0.039) 

0.913 

(0.028) 

Average 0.907 

(0.031) 

0.877 

(0.032) 

0.919 

(0.027) 

0.876 

(0.033) 

0.897 

(0.032) 

Number of 14-Day-Old 

Surviving Chicks as A 

Percentage of Number of 

Eggs Set from Weeks 7 

and 8 

Lab1_ST 0.796 

(0.049) 

0.729 

(0.058) 

0.903 

(0.027) 

0.708 

(0.069) 

0.749 

(0.074) 

Lab2_SV 0.846 

(0.044) 

0.778 

(0.048) 

0.743 

(0.037) 

0.818 

(0.049) 

0.871 

(0.029) 

Lab3_WLI 0.860 

(0.051) 

0.910 

(0.025) 

0.891 

(0.025) 

0.826 

(0.054) 

0.873 

(0.038) 

Average 0.835 

(0.045) 

0.821 

(0.043) 

0.851 

(0.039) 

0.791 

(0.048) 

0.846 

(0.043) 

Eggs Hatched Per Hen Per 

Day 

Lab1_ST 0.667 

(0.056) 

0.603 

(0.060) 

0.756 

(0.029) 

0.607 

(0.067) 

0.603 

(0.075) 

Lab2_SV 0.671 

(0.060) 

0.655 

(0.046) 

0.669 

(0.049) 

0.667 

(0.054) 

0.719 

(0.048) 

Lab3_WLI 0.817 

(0.051) 

0.767 

(0.056) 

0.763 

(0.059) 

0.773 

(0.052) 

0.790 

(0.041) 

Average 0.722 

(0.047) 

0.678 

(0.046) 

0.752 

(0.042) 

0.688 

(0.048) 

0.721 

(0.045) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

87 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Reproduction (Continued) 

Number of 14-Day-Old 

Surviving Chicks Per Hen 

Per Day 

Lab1_ST 0.639 

(0.055) 

0.594 

(0.060) 

0.756 

(0.029) 

0.585 

(0.065) 

0.624 

(0.069) 

Lab2_SV 0.651 

(0.058) 

0.630 

(0.047) 

0.631 

(0.050) 

0.647 

(0.052) 

0.694 

(0.049) 

Lab3_WLI 0.777 

(0.052) 

0.757 

(0.055) 

0.710 

(0.055) 

0.731 

(0.052) 

0.762 

(0.047) 

Average 0.690 

(0.045) 

0.662 

(0.045) 

0.718 

(0.041) 

0.660 

(0.045) 

0.702 

(0.045) 

Egg Shell and Weight

Pen Mean Egg Shell 

Thickness (mm) 

Lab1_ST 0.204 

(0.003) 

0.201 

(0.004) 

0.206 

(0.004) 

0.212 

(0.003) 

0.202 

(0.004) 

Lab2_SV 0.207 

(0.004) 

0.203 

(0.004) 

0.201 

(0.004) 

0.201 

(0.004) 

0.206 

(0.003) 

Lab3_WLI 0.229 

(0.003) 

0.236 

(0.004) 

0.237 

(0.006) 

0.239 

(0.003) 

0.237 

(0.003) 

Average 0.212 

(0.010) 

0.213 

(0.010) 

0.214 

(0.011) 

0.218 

(0.010) 

0.216 

(0.010) 

Pen Mean Egg Content 

Weight - Albumin (g) 

Lab1_ST 6.475 

(0.131) 

6.743 

(0.266) 

6.848 

(0.153) 

6.801 

(0.134) 

6.789 

(0.133) 

Lab2_SV 4.456 

(0.208) 

5.067 

(0.108) 

4.781 

(0.135) 

4.857 

(0.191) 

5.210 

(0.125) 

Lab3_WLI 4.825 

(0.103) 

4.820 

(0.140) 

4.794 

(0.109) 

4.846 

(0.107) 

4.885 

(0.076) 

Average 5.324 

(0.624) 

5.566 

(0.626) 

5.464 

(0.624) 

5.512 

(0.624) 

5.612 

(0.623) 

Pen Mean Egg Content 

Weight - Yolk (g) 

Lab1_ST 3.821 

(0.085) 

3.834 

(0.121) 

3.902 

(0.080) 

3.873 

(0.066) 

3.825 

(0.093) 

Lab2_SV 3.784 

(0.106) 

3.941 

(0.103) 

3.905 

(0.073) 

4.274 

(0.136) 

4.005 

(0.116) 

Lab3_WLI 3.275 

(0.091) 

3.190 

(0.062) 

3.256 

(0.085) 

3.347 

(0.059) 

3.179 

(0.067) 

Average 3.638 

(0.226) 

3.641 

(0.225) 

3.684 

(0.224) 

3.773 

(0.223) 

3.647 

(0.225) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

88 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Egg Shell and Weight (Continued) 

Pen Mean Egg Content 

Weight - Combined (g) 

Lab1_ST 10.295 

(0.193) 

10.575 

(0.366) 

10.748 

(0.222) 

10.674 

(0.173) 

10.613 

(0.193) 

Lab2_SV 8.239 

(0.297) 

9.009 

(0.174) 

8.686 

(0.182) 

9.131 

(0.272) 

9.214 

(0.232) 

Lab3_WLI 8.100 

(0.133) 

8.011 

(0.156) 

8.049 

(0.173) 

8.193 

(0.126) 

8.064 

(0.123) 

Average 9.016 

(0.741) 

9.193 

(0.742) 

9.140 

(0.741) 

9.306 

(0.740) 

9.241 

(0.739) 

First Foam First Egg Cloacal Size

Age in Days at First Foam 

- Males 

Lab1_ST 34.500 

(0.283) 

34.611 

(0.405) 

35.389 

(0.657) 

33.944 

(0.318) 

33.944 

(0.308) 

Lab2_SV 42.611 

(3.422) 

38.278 

(0.709) 

38.500 

(0.506) 

37.722 

(0.547) 

37.889 

(0.788) 

Lab3_WLI 37.688 

(0.810) 

37.200 

(1.787) 

35.765 

(1.006) 

36.765 

(0.991) 

36.667 

(0.900) 

Average 36.677 

(1.161) 

36.669 

(1.178) 

36.829 

(1.182) 

36.049 

(1.155) 

36.064 

(1.159) 

Age in Days at First Egg - 

Females 

Lab1_ST 42.722 

(0.666) 

42.611 

(0.789) 

43.556 

(0.841) 

44.556 

(0.841) 

42.588 

(0.840) 

Lab2_SV 48.278 

(1.885) 

46.111 

(1.093) 

51.889 

(2.737) 

45.500 

(1.764) 

46.000 

(1.360) 

Lab3_WLI 45.063 

(1.047) 

44.333 

(2.294) 

43.625 

(1.291) 

46.765 

(1.773) 

43.889 

(0.840) 

Average 44.743 

(1.177) 

44.273 

(1.210) 

45.086 

(1.251) 

45.898 

(1.257) 

44.142 

(1.169) 

Cloacal Area (cm2) at 4 

Weeks - Males 

Lab1_ST 0.292 

(0.020) 

0.267 

(0.015) 

0.278 

(0.016) 

0.299 

(0.023) 

0.274 

(0.016) 

Lab2_SV 0.222 

(0.018) 

0.238 

(0.026) 

0.282 

(0.024) 

0.220 

(0.035) 

0.238 

(0.034) 

Lab3_WLI 0.253 

(0.021) 

0.393 

(0.054) 

0.363 

(0.044) 

0.391 

(0.043) 

0.428 

(0.045) 

Average 0.256 

(0.036) 

0.287 

(0.038) 

0.305 

(0.037) 

0.301 

(0.039) 

0.302 

(0.038) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

89 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

First Foam First Egg Cloacal Size (Continued) 

Cloacal Area (cm2) at 5 

Weeks - Males 

Lab1_ST 0.372 

(0.030) 

0.351 

(0.019) 

0.378 

(0.021) 

0.398 

(0.019) 

0.385 

(0.020) 

Lab2_SV 0.279 

(0.029) 

0.302 

(0.027) 

0.328 

(0.037) 

0.298 

(0.037) 

0.318 

(0.031) 

Lab3_WLI 0.539 

(0.073) 

0.568 

(0.075) 

0.499 

(0.059) 

0.483 

(0.055) 

0.381 

(0.035) 

Average 0.369 

(0.047) 

0.365 

(0.045) 

0.388 

(0.046) 

0.393 

(0.045) 

0.371 

(0.045) 

Cloacal Area (cm2) at 6 

Weeks - Males 

Lab1_ST 0.558 

(0.029) 

0.499 

(0.031) 

0.544 

(0.029) 

0.574 

(0.032) 

0.579 

(0.035) 

Lab2_SV 0.423 

(0.029) 

0.447 

(0.033) 

0.434 

(0.041) 

0.433 

(0.041) 

0.421 

(0.040) 

Lab3_WLI 0.664 

(0.041) 

0.736 

(0.048) 

0.848 

(0.054) 

0.636 

(0.049) 

0.551 

(0.042) 

Average 0.549 

(0.079) 

0.555 

(0.079) 

0.595 

(0.080) 

0.551 

(0.080) 

0.520 

(0.080) 

Cloacal Area (cm2) at 7 

Weeks - Males 

Lab1_ST NA NA NA NA NA 

Lab2_SV NA NA NA NA NA 

Lab3_WLI 0.593 

(0.035) 

0.601 

(0.035) 

0.509 

(0.033) 

0.474 

(0.028) 

0.564 

(0.030) 

Average NA NA NA NA NA 

Cloacal Area (cm2) at 8 

Weeks - Males 

Lab1_ST NA NA NA NA NA 

Lab2_SV NA NA NA NA NA 

Lab3_WLI 0.582 

(0.025) 

0.562 

(0.027) 

0.567 

(0.025) 

0.511 

(0.023) 

0.522 

(0.013) 

Average NA NA NA NA NA 

Cloacal Area (cm2) at 9 

Weeks - Males 

Lab1_ST NA NA NA NA NA 

Lab2_SV NA NA NA NA NA 

Lab3_WLI 0.589 

(0.023) 

0.564 

(0.024) 

0.581 

(0.021) 

0.532 

(0.014) 

0.526 

(0.012) 

Average NA NA NA NA NA 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

90 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

First Foam First Egg Cloacal Size (Continued) 

Cloacal Area (cm2) at 4 

Weeks - Females 

Lab1_ST 0.274 

(0.019) 

0.313 

(0.020) 

0.288 

(0.021) 

0.275 

(0.016) 

0.289 

(0.024) 

Lab2_SV 0.213 

(0.029) 

0.311 

(0.037) 

0.268 

(0.028) 

0.312 

(0.030) 

0.286 

(0.032) 

Lab3_WLI 0.244 

(0.021) 

0.294 

(0.031) 

0.270 

(0.015) 

0.304 

(0.044) 

0.292 

(0.031) 

Average 0.251 

(0.013) 

0.308 

(0.015) 

0.275 

(0.011) 

0.285 

(0.013) 

0.289 

(0.016) 

Cloacal Area (cm2) at 5 

Weeks - Females 

Lab1_ST 0.402 

(0.051) 

0.534 

(0.056) 

0.509 

(0.046) 

0.423 

(0.032) 

0.499 

(0.039) 

Lab2_SV 0.111 

(0.014) 

0.144 

(0.019) 

0.164 

(0.027) 

0.157 

(0.018) 

0.163 

(0.031) 

Lab3_WLI 0.524 

(0.086) 

0.590 

(0.064) 

0.453 

(0.045) 

0.631 

(0.148) 

0.546 

(0.088) 

Average 0.336 

(0.117) 

0.392 

(0.117) 

0.379 

(0.117) 

0.368 

(0.117) 

0.398 

(0.118) 

Cloacal Area (cm2) at 6 

Weeks - Females 

Lab1_ST 0.521 

(0.040) 

0.702 

(0.042) 

0.676 

(0.041) 

0.765 

(0.054) 

0.626 

(0.052) 

Lab2_SV 0.103 

(0.010) 

0.123 

(0.014) 

0.119 

(0.015) 

0.134 

(0.017) 

0.102 

(0.011) 

Lab3_WLI 0.956 

(0.120) 

0.763 

(0.082) 

0.771 

(0.054) 

0.803 

(0.086) 

0.654 

(0.065) 

Average 0.470 

(0.202) 

0.528 

(0.201) 

0.519 

(0.201) 

0.554 

(0.202) 

0.476 

(0.201) 

Cloacal Area (cm2) at 7 

Weeks - Females 

Lab1_ST NA NA NA NA NA 

Lab2_SV NA NA NA NA NA 

Lab3_WLI 0.652 

(0.044) 

0.634 

(0.048) 

0.691 

(0.051) 

0.689 

(0.035) 

0.597 

(0.031) 

Average NA NA NA NA NA 

Cloacal Area (cm2) at 8 

Weeks - Females 

Lab1_ST NA NA NA NA NA 

Lab2_SV NA NA NA NA NA 

Lab3_WLI 0.638 

(0.035) 

0.606 

(0.035) 

0.739 

(0.029) 

0.675 

(0.028) 

0.623 

(0.041) 

Average NA NA NA NA NA 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

91 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

First Foam First Egg Cloacal Size (Continued) 

Cloacal Area (cm2) at 9 

Weeks - Females 

Lab1_ST NA NA NA NA NA 

Lab2_SV NA NA NA NA NA 

Lab3_WLI 0.679 

(0.033) 

0.607 

(0.030) 

0.734 

(0.028) 

0.656 

(0.022) 

0.639 

(0.025) 

Average NA NA NA NA NA 

Mating Behavior

Length of Time to First 

Neck Grab (sec) - Day 1 

Lab1_ST 21.286 

(11.720) 

21.714 

(18.064) 

75.286 

(42.417) 

63.143 

(40.678) 

50.429 

(41.689) 

Lab2_SV 15.143 

(6.780) 

4.143 

(1.223) 

14.857 

(7.799) 

6.571 

(1.251) 

29.714 

(15.240) 

Lab3_WLI 7.571 

(1.571) 

50.714 

(41.590) 

9.857 

(1.471) 

59.667 

(48.168) 

5.833 

(1.302) 

Average 14.017 

(5.629) 

4.897 

(5.765) 

16.239 

(5.682) 

7.321 

(5.785) 

12.340 

(5.689) 

Length of Time to First 

Neck Grab (sec) - Day 2 

Lab1_ST 6.571 

(2.707) 

2.000 

(0.535) 

45.143 

(27.424) 

27.286 

(24.790) 

9.857 

(4.356) 

Lab2_SV 8.286 

(1.848) 

9.143 

(4.496) 

6.429 

(2.467) 

5.143 

(1.056) 

17.714 

(12.453) 

Lab3_WLI 5.286 

(1.304) 

45.714 

(42.384) 

4.429 

(0.751) 

57.000 

(48.716) 

6.833 

(2.428) 

Average 6.516 

(1.279) 

2.273 

(1.242) 

5.247 

(1.226) 

4.261 

(1.494) 

8.420 

(2.266) 

Length of Time to First 

Neck Grab (sec) - Day 3 

Lab1_ST 3.000 

(0.309) 

2.857 

(0.829) 

4.429 

(1.307) 

1.857 

(0.404) 

5.571 

(1.270) 

Lab2_SV 4.286 

(1.063) 

5.429 

(3.116) 

7.143 

(3.832) 

3.143 

(0.705) 

3.000 

(0.488) 

Lab3_WLI 3.143 

(0.340) 

45.857 

(42.364) 

3.143 

(0.340) 

52.833 

(49.434) 

3.333 

(0.558) 

Average 3.118 

(0.223) 

3.042 

(0.801) 

3.253 

(0.328) 

2.178 

(0.351) 

3.332 

(0.353) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

92 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued) 

Change in Length of Time 

to First Neck Grab (sec) 

from Day 1 to Day 3 

Lab1_ST -18.286 

(11.478) 

-18.857 

(17.872) 

-70.857 

(42.509) 

-61.286 

(40.360) 

-44.857 

(40.635) 

Lab2_SV -10.857 

(5.881) 

1.286 

(3.257) 

-7.714 

(5.809) 

-3.429 

(1.412) 

-26.714 

(14.885) 

Lab3_WLI -4.429 

(1.412) 

-4.857 

(1.779) 

-6.714 

(1.700) 

-6.833 

(3.219) 

-2.500 

(1.628) 

Average -5.887 

(2.346) 

-3.025 

(2.264) 

-7.103 

(2.516) 

-4.638 

(2.152) 

-3.442 

(2.666) 

Length of Time to First 

Mount (sec) - Day 1 

Lab1_ST 61.571 

(40.199) 

25.857 

(17.686) 

76.571 

(42.061) 

65.714 

(40.132) 

54.286 

(41.126) 

Lab2_SV 39.571 

(16.959) 

5.143 

(1.164) 

20.286 

(8.190) 

27.143 

(6.745) 

46.571 

(16.569) 

Lab3_WLI 57.143 

(40.865) 

56.714 

(40.766) 

18.143 

(5.986) 

61.833 

(47.724) 

11.500 

(3.990) 

Average 45.740 

(16.504) 

11.620 

(9.090) 

21.427 

(8.700) 

32.059 

(11.529) 

21.765 

(9.427) 

Length of Time to First 

Mount (sec) - Day 2 

Lab1_ST 13.143 

(5.157) 

3.714 

(1.128) 

47.286 

(27.736) 

30.143 

(24.656) 

11.000 

(4.326) 

Lab2_SV 15.000 

(5.196) 

47.714 

(42.058) 

9.286 

(2.652) 

11.286 

(2.688) 

21.429 

(11.864) 

Lab3_WLI 51.286 

(41.543) 

47.000 

(42.171) 

8.714 

(1.822) 

58.167 

(48.492) 

11.333 

(2.275) 

Average 14.351 

(3.646) 

3.777 

(1.127) 

9.010 

(1.500) 

11.648 

(2.668) 

11.546 

(1.985) Yes 

Length of Time to First 

Mount (sec) - Day 3 

Lab1_ST 5.714 

(1.392) 

3.857 

(0.911) 

5.143 

(1.299) 

2.429 

(0.612) 

6.571 

(1.152) 

Lab2_SV 15.714 

(11.401) 

49.429 

(41.783) 

49.286 

(41.856) 

8.571 

(2.991) 

8.000 

(3.132) 

Lab3_WLI 5.000 

(0.900) 

46.571 

(42.245) 

4.714 

(0.421) 

54.000 

(49.205) 

5.667 

(0.211) 

Average 5.256 

(0.754) 

3.899 

(0.911) 

4.759 

(0.400) 

2.683 

(0.599) 

5.706 

(0.207) Yes 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

93 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued) 

Change in Length of Time 

to First Mount (sec) from 

Day 1 to Day 3 

Lab1_ST -55.857 

(40.219) 

-22.000 

(17.502) 

-71.429 

(42.223) 

-63.286 

(39.763) 

-47.714 

(40.255) 

Lab2_SV -23.857 

(14.175) 

44.286 

(41.986) 

29.000 

(38.414) 

-18.571 

(7.690) 

-38.571 

(17.735) 

Lab3_WLI -52.143 

(40.716) 

-10.143 

(4.044) 

-13.429 

(6.019) 

-7.833 

(2.868) 

-5.833 

(4.094) 

Average -29.125 

(14.009) 

-16.550 

(7.297) 

-20.132 

(8.585) 

-15.379 

(6.658) 

-14.369 

(7.384) 

Number of Mount 

Attempts (count) - Day 1 

Lab1_ST 7.857 

(1.262) 

9.286 

(2.212) 

8.286 

(1.924) 

8.143 

(2.604) 

11.143 

(2.595) 

Lab2_SV 5.286 

(1.040) 

5.857 

(0.800) 

5.857 

(1.779) 

6.571 

(1.445) 

5.571 

(1.288) 

Lab3_WLI 5.429 

(1.088) 

5.000 

(1.113) 

5.714 

(0.918) 

3.167 

(0.792) 

7.833 

(1.869) 

Average 6.185 

(1.149) 

6.430 

(1.160) 

6.749 

(1.231) 

5.076 

(1.196) 

7.394 

(1.379) 

Number of Mount 

Attempts (count) - Day 2 

Lab1_ST 8.286 

(2.212) 

9.286 

(1.584) 

5.857 

(1.610) 

7.714 

(2.598) 

9.143 

(1.857) 

Lab2_SV 12.429 

(3.351) 

6.143 

(1.487) 

7.714 

(1.911) 

11.143 

(2.219) 

4.571 

(0.948) 

Lab3_WLI 3.714 

(1.085) 

4.000 

(1.175) 

5.286 

(1.017) 

3.000 

(0.856) 

5.833 

(1.249) 

Average 6.997 

(1.864) 

6.403 

(1.666) 

6.330 

(1.691) 

6.612 

(1.785) 

6.219 

(1.646) 

Number of Mount 

Attempts (count) - Day 3 

Lab1_ST 6.000 

(1.195) 

8.714 

(2.286) 

6.571 

(2.487) 

5.429 

(1.494) 

6.143 

(1.184) 

Lab2_SV 13.429 

(3.123) 

6.571 

(1.525) 

11.714 

(2.909) 

14.143 

(2.262) 

9.286 

(1.886) 

Lab3_WLI 5.286 

(1.554) 

4.429 

(1.172) 

5.000 

(0.724) 

3.000 

(0.894) 

5.667 

(1.406) 

Average 7.448 

(1.987) 

6.159 

(1.942) 

7.429 

(1.978) 

6.520 

(1.908) 

7.078 

(1.911) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

94 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued) 

Change in Number of 

Mount Attempts (count) 

from Day 1 to Day 3 

Lab1_ST -1.857 

(1.945) 

-0.571 

(2.298) 

-1.714 

(1.769) 

-2.714 

(3.393) 

-5.000 

(2.093) 

Lab2_SV 8.143 

(2.272) 

0.714 

(1.040) 

5.857 

(2.064) 

7.571 

(2.644) 

3.714 

(0.892) 

Lab3_WLI -0.143 

(1.388) 

-0.571 

(0.782) 

-0.714 

(0.993) 

-0.167 

(0.654) 

-2.167 

(2.701) 

Average 1.749 

(2.379) 

-0.576 

(2.260) 

0.941 

(2.315) 

1.364 

(2.386) 

-0.775 

(2.358) 

Number of Cloacal 

Contacts (count) - Day 1 

Lab1_ST 1.714 

(0.565) 

0.857 

(0.404) 

2.143 

(0.508) 

1.714 

(0.565) 

2.000 

(0.845) 

Lab2_SV 2.857 

(0.595) 

3.286 

(0.522) 

3.857 

(0.738) 

3.571 

(1.043) 

3.143 

(0.911) 

Lab3_WLI 2.714 

(1.017) 

2.571 

(0.528) 

2.286 

(0.360) 

2.167 

(0.601) 

4.000 

(1.183) 

Average 2.355 

(0.617) 

2.137 

(0.555) 

2.596 

(0.561) 

2.401 

(0.620) 

2.893 

(0.731) 

Number of Cloacal 

Contacts (count) - Day 2 

Lab1_ST 2.143 

(0.769) 

3.857 

(0.634) 

3.714 

(0.837) 

2.714 

(0.837) 

3.714 

(0.644) 

Lab2_SV 5.429 

(1.631) 

3.571 

(1.110) 

4.000 

(0.756) 

3.714 

(0.865) 

3.000 

(0.309) 

Lab3_WLI 3.143 

(0.986) 

3.571 

(0.922) 

3.000 

(0.488) 

2.333 

(0.667) 

3.333 

(0.760) 

Average 2.874 

(0.569) 

3.730 

(0.472) 

3.375 

(0.368) 

2.810 

(0.447) 

3.157 

(0.261) 

Number of Cloacal 

Contacts (count) - Day 3 

Lab1_ST 2.857 

(0.769) 

3.714 

(0.969) 

3.286 

(0.522) 

3.286 

(1.017) 

3.429 

(0.528) 

Lab2_SV 7.571 

(1.462) 

3.714 

(0.747) 

4.286 

(1.358) 

5.143 

(0.705) 

6.714 

(1.375) 

Lab3_WLI 3.000 

(0.488) 

2.857 

(0.769) 

3.714 

(0.421) 

2.500 

(0.671) 

3.333 

(0.558) 

Average 3.757 

(0.739) 

3.294 

(0.769) 

4.072 

(0.699) 

3.582 

(0.751) 

4.074 

(0.722) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

95 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued) 

Change in Number of 

Cloacal Contacts (count) 

from Day 1 to Day 3 

Lab1_ST 1.143 

(0.508) 

2.857 

(0.911) 

1.143 

(0.508) 

1.571 

(0.751) 

1.429 

(0.528) 

Lab2_SV 4.714 

(1.459) 

0.429 

(0.429) 

0.429 

(0.896) 

1.571 

(1.556) 

3.571 

(1.395) 

Lab3_WLI 0.286 

(1.085) 

0.286 

(0.808) 

1.429 

(0.612) 

0.333 

(0.422) 

-0.667 

(1.256) 

Average 1.498 

(0.698) 

0.979 

(0.608) 

1.075 

(0.594) 

0.965 

(0.643) 

1.334 

(0.715) 

Number of Crows (Cock) 

or Fluffs (Hen) (count) - 

Day 1 

Lab1_ST 6.429 

(0.922) 

4.000 

(1.215) 

3.286 

(0.606) 

4.143 

(1.143) 

5.571 

(0.841) 

Lab2_SV 7.714 

(1.340) 

3.571 

(1.043) 

7.429 

(1.541) 

3.429 

(0.571) 

3.000 

(0.724) 

Lab3_WLI 0.857 

(0.261) 

1.571 

(0.922) 

1.714 

(1.128) 

1.833 

(0.749) 

1.667 

(0.760) 

Average 4.545 

(1.359) 

3.057 

(1.403) 

3.710 

(1.407) 

3.103 

(1.350) 

3.381 

(1.340) 

Number of Crows (Cock) 

or Fluffs (Hen) (count) - 

Day 2 

Lab1_ST 3.286 

(0.474) 

4.857 

(0.962) 

5.143 

(0.800) 

2.714 

(0.606) 

4.714 

(0.993) 

Lab2_SV 5.000 

(1.327) 

5.000 

(0.816) 

4.000 

(0.900) 

4.286 

(0.714) 

4.286 

(0.714) 

Lab3_WLI 1.000 

(0.436) 

0.429 

(0.202) 

1.857 

(0.738) 

0.833 

(0.654) 

0.833 

(0.543) 

Average 2.903 

(1.178) 

2.940 

(1.170) 

3.742 

(1.216) 

2.571 

(1.185) 

3.141 

(1.196) 

Number of Crows (Cock) 

or Fluffs (Hen) (count) - 

Day 3 

Lab1_ST 2.429 

(0.429) 

2.857 

(0.705) 

4.429 

(0.369) 

2.714 

(0.565) 

3.857 

(0.459) 

Lab2_SV 7.571 

(1.212) 

8.429 

(1.172) 

9.143 

(1.487) 

6.857 

(1.223) 

8.714 

(0.644) 

Lab3_WLI 0.714 

(0.286) 

2.000 

(1.091) 

1.143 

(0.261) 

1.333 

(0.615) 

2.167 

(0.792) 

Average 3.523 

(2.044) 

4.241 

(2.092) 

4.655 

(2.043) 

3.790 

(2.064) 

4.869 

(2.050) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

96 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued) 

Change in Number of 

Crows (Cock) or Fluffs 

(Hen) (count) from Day 1 

to Day 3 

Lab1_ST -4.000 

(1.024) 

-1.143 

(1.033) 

1.143 

(0.553) 

-1.429 

(1.110) 

-1.714 

(1.149) 

Lab2_SV -0.143 

(1.779) 

4.857 

(1.565) 

1.714 

(1.629) 

3.429 

(0.922) 

5.714 

(0.865) 

Lab3_WLI -0.143 

(0.340) 

0.429 

(0.369) 

-0.571 

(1.020) 

-0.500 

(0.428) 

0.500 

(0.806) 

Average -0.960 

(1.625) 

1.215 

(1.617) 

1.201 

(1.630) 

0.464 

(1.588) 

1.589 

(1.608) 

Thyroid Weight

15 Day Embryo (ED15) 

Thyroid Weight (mg) 

Lab1_ST 0.991 

(0.143) 

1.323 

(0.091) 

1.269 

(0.160) 

1.200 

(0.111) 

1.162 

(0.124) 

Lab2_SV 2.048 

(0.146) 

2.198 

(0.163) 

2.173 

(0.363) 

1.868 

(0.139) 

1.750 

(0.159) 

Lab3_WLI 2.371 

(0.208) 

2.850 

(0.132) 

2.560 

(0.182) 

2.912 

(0.169) 

3.000 

(0.268) 

Average 1.839 

(0.450) 

2.109 

(0.446) 

1.960 

(0.456) 

1.972 

(0.448) 

1.902 

(0.451) 

Adult Left Thyroid Weight 

(mg) - Males 

Lab1_ST 10.022 

(1.061) 

9.531 

(1.054) 

10.178 

(1.372) 

11.150 

(1.100) 

9.969 

(1.020) 

Lab2_SV 6.807 

(0.681) 

8.647 

(1.373) 

8.873 

(1.162) 

11.922 

(1.539) 

9.636 

(1.496) 

Lab3_WLI 5.533 

(0.679) 

6.800 

(1.138) 

6.394 

(0.738) 

6.535 

(0.700) 

7.011 

(0.712) 

Average 7.234 

(1.161) 

8.292 

(1.267) 

8.418 

(1.219) 

9.124 

(1.214) 

8.810 

(1.209) 

Adult Left Thyroid Weight 

(mg) - Females 

Lab1_ST 13.322 

(1.203) 

12.294 

(0.761) 

10.239 

(1.133) 

14.556 

(1.851) 

19.425 

(2.853) 

Lab2_SV 15.460 

(1.893) 

12.057 

(1.415) 

12.836 

(1.639) 

14.317 

(2.054) 

13.425 

(1.705) 

Lab3_WLI 6.275 

(0.743) 

6.219 

(0.471) 

6.094 

(0.887) 

7.982 

(1.013) 

7.344 

(0.794) 

Average 11.003 

(2.239) 

10.389 

(2.193) 

9.720 

(2.246) 

12.266 

(2.304) 

11.850 

(2.270) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

97 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Sex Ratio

Ratio of Number of Males 

to Total Number of 

Offspring (%) 

Lab1_ST 48.413 

(6.569) 

45.563 

(5.276) 

47.676 

(3.915) 

39.459 

(3.860) 

51.565 

(5.165) 

Lab2_SV 54.965 

(4.077) 

48.276 

(4.481) 

54.199 

(5.063) 

48.718 

(4.736) 

46.915 

(3.838) 

Lab3_WLI 52.979 

(4.702) 

51.256 

(5.317) 

51.615 

(3.597) 

49.144 

(6.827) 

48.169 

(2.598) 

Average 53.026 

(2.819) 

48.325 

(2.900) 

50.769 

(2.381) 

44.179 

(2.772) 

48.273 

(2.034) 

Bio Chem

Egg Yolk Estradiol (pg/g) 

Lab1_ST 92.541 

(13.371) 

84.648 

(8.491) 

69.838 

(10.750) 

80.260 

(5.120) 

84.588 

(7.629) 

Lab2_SV 43.908 

(13.558) 

43.095 

(7.906) 

25.923 

(5.182) 

23.293 

(3.758) 

24.767 

(6.483) 

Lab3_WLI 66.995 

(10.430) 

81.308 

(12.985) 

86.645 

(4.554) 

79.186 

(8.847) 

58.943 

(7.847) 

Average 65.941 

(18.336) 

70.123 

(17.768) 

63.395 

(17.319) 

59.769 

(17.232) 

56.251 

(17.421) 

Egg Yolk Testosterone 

(pg/g) 

Lab1_ST 5.171 

(0.672) 

2.990 

(0.511) 

5.720 

(1.494) 

3.809 

(0.439) 

5.277 

(0.914) 

Lab2_SV 1.971 

(0.258) 

2.728 

(0.691) 

1.754 

(0.378) 

1.856 

(0.280) 

1.652 

(0.261) 

Lab3_WLI 2.222 

(0.373) 

2.071 

(0.211) 

2.053 

(0.388) 

1.932 

(0.376) 

2.068 

(0.289) 

Average 2.858 

(0.708) 

2.538 

(0.706) 

2.621 

(0.730) 

2.562 

(0.704) 

2.574 

(0.705) 

Plasma Thyroid Hormone 

T3 - Adult Males (ng/mL) 

Lab1_ST 0.718 

(0.075) 

0.706 

(0.074) 

0.533 

(0.096) 

0.844 

(0.173) 

0.689 

(0.093) 

Lab2_SV 0.620 

(0.044) 

0.528 

(0.261) 

0.873 

(0.102) 

0.844 

(0.225) 

1.230 

(0.176) 

Lab3_WLI 0.992 

(0.129) 

1.106 

(0.180) 

1.027 

(0.065) 

1.056 

(0.134) 

1.029 

(0.098) 

Average 0.755 

(0.120) 

0.827 

(0.139) 

0.816 

(0.121) 

0.917 

(0.148) 

0.924 

(0.129) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

98 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Bio Chem (Continued)

Plasma Thyroid Hormone 

T3 - Adult Females 

(ng/mL) 

Lab1_ST 0.761 

(0.098) 

0.885 

(0.081) 

0.673 

(0.149) 

0.805 

(0.124) 

0.814 

(0.137) 

Lab2_SV 0.755 

(0.064) 

0.710 

(0.131) 

1.180 

(0.110) 

1.193 

(0.077) 

0.981 

(0.108) 

Lab3_WLI 0.927 

(0.076) 

0.864 

(0.102) 

0.963 

(0.057) 

1.067 

(0.092) 

0.744 

(0.098) 

Average 0.813 

(0.077) 

0.837 

(0.086) 

0.965 

(0.085) 

1.048 

(0.083) 

0.844 

(0.090) 

Plasma Thyroid Hormone 

T4 - Adult Males (ng/mL) 

Lab1_ST 6.385 

(1.309) 

5.159 

(1.136) 

4.267 

(1.632) 

8.721 

(1.753) 

3.298 

(0.861) 

Lab2_SV 1.829 

(0.517) 

0.916 

(0.153) 

3.340 

(1.290) 

3.973 

(1.073) 

3.155 

(0.699) 

Lab3_WLI 3.574 

(1.131) 

3.759 

(1.078) 

2.516 

(0.595) 

3.159 

(0.873) 

1.830 

(0.452) 

Average 3.636 

(1.149) 

2.894 

(1.109) 

3.337 

(1.193) 

4.829 

(1.215) 

2.775 

(1.078) 

Plasma Thyroid Hormone 

T4 - Adult Females 

(ng/mL) 

Lab1_ST 2.843 

(0.730) 

4.620 

(0.730) 

3.353 

(1.041) 

7.286 

(2.537) 

4.429 

(0.775) 

Lab2_SV 2.590 

(0.779) 

1.437 

(0.674) 

4.914 

(1.351) 

4.454 

(1.243) 

5.688 

(1.380) 

Lab3_WLI 1.930 

(0.365) 

1.890 

(0.300) 

2.620 

(1.475) 

1.974 

(0.581) 

0.763 

(0.000) 

Average 2.510 

(0.965) 

2.662 

(0.954) 

3.515 

(1.158) 

3.793 

(1.138) 

3.079 

(0.997) 

Plasma Sex Hormone 

Estradiol - Adult Males 

(pg/mL) 

Lab1_ST 5.385 

(0.764) 

3.676 

(0.963) 

2.474 

(0.512) 

4.187 

(0.839) 

6.609 

(1.177) 

Lab2_SV 10.140 

(4.754) 

3.743 

(1.038) 

3.802 

(1.293) 

6.841 

(1.093) 

3.934 

(0.842) 

Lab3_WLI 6.391 

(2.691) 

3.700 

(0.000) 

3.700 

(0.000) 

3.700 

(0.000) 

3.700 

(0.000) 

Average 5.704 

(0.977) 

3.703 

(0.570) 

3.281 

(0.538) 

4.390 

(0.563) 

4.243 

(0.568) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

99 

Table 15. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F1 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Bio Chem (Continued)

Plasma Sex Hormone 

Estradiol - Adult Females 

(pg/mL) 

Lab1_ST 22.013 

(4.460) 

18.173 

(4.878) 

22.329 

(5.155) 

15.928 

(2.876) 

21.871 

(6.048) 

Lab2_SV 14.819 

(1.889) 

10.840 

(3.598) 

16.369 

(0.765) 

15.642 

(4.653) 

15.312 

(1.236) 

Lab3_WLI 17.685 

(3.163) 

20.042 

(3.955) 

20.266 

(2.126) 

30.026 

(5.899) 

24.402 

(3.788) 

Average 17.593 

(2.309) 

16.026 

(2.875) 

19.128 

(1.970) 

17.839 

(2.862) 

18.637 

(2.165) 

Plasma Sex Hormone 

Testosterone - Adult 

Males (pg/mL) 

Lab1_ST 3237.80 

(273.449) 

2503.84 

(991.019) 

2018.28 

(674.302) 

2030.23 

(656.375) 

2717.24 

(845.548) 

Lab2_SV 478.438 

(127.383) 

625.846 

(117.731) 

1004.41 

(242.910) 

888.730 

(406.711) 

2574.34 

(751.450) 

Lab3_WLI 1596.37 

(549.294) 

3253.82 

(679.184) 

2786.43 

(953.096) 

959.411 

(316.315) 

3435.18 

(872.284) 

Average 1788.25 

(508.925) 

1859.29 

(557.788) 

1847.21 

(570.363) 

1239.73 

(540.443) 

2937.72 

(670.774) 

Plasma Sex Hormone 

Testosterone - Adult 

Females (pg/mL) 

Lab1_ST 531.493 

(198.570) 

462.757 

(172.478) 

958.046 

(333.005) 

315.118 

(82.894) 

541.602 

(187.127) 

Lab2_SV 392.223 

(57.767) 

382.268 

(214.027) 

369.583 

(153.880) 

336.661 

(163.380) 

199.550 

(44.787) 

Lab3_WLI 276.222 

(138.471) 

957.498 

(381.354) 

796.197 

(293.006) 

1157.08 

(330.153) 

991.428 

(322.689) 

Average 403.421 

(143.290) 

530.824 

(182.568) 

625.543 

(185.290) 

453.014 

(157.036) 

444.834 

(154.746) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

100 

Table 16. Mean and Standard Errors and Comparisons of Avian Vinclozolin Concentrations to 

Control across Laboratories, F2 Generationf

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Body Weight

Mean Offspring Body 

Weight by Pen (g) Day 0 

Lab1_ST 8.494 

(0.177) 

8.806 

(0.210) 

8.524 

(0.169) 

8.453 

(0.128) 

8.440 

(0.200) 

Lab2_SV 9.150 

(0.175) 

9.524 

(0.191) 

9.006 

(0.124) 

9.578 

(0.208) 

9.565 

(0.160) 

Lab3_WLI 6.544 

(0.147) 

6.687 

(0.095) 

6.340 

(0.137) 

6.571 

(0.144) 

6.389 

(0.081) 

Average 8.074 

(0.855) 

8.328 

(0.854) 

7.931 

(0.854) 

8.162 

(0.854) 

8.096 

(0.853) 

Mean Offspring Body 

Weight by Pen (g) Day 14 

Lab1_ST 82.617 

(1.543) 

83.763 

(1.354) 

83.276 

(1.059) 

83.418 

(0.922) 

80.387 

(1.761) 

Lab2_SV 70.372 

(1.357) 

71.165 

(2.071) 

72.447 

(1.052) 

72.217 

(1.179) 

72.482 

(1.285) 

Lab3_WLI 49.563 

(1.045) 

50.400 

(0.786) 

49.200 

(0.932) 

50.412 

(1.225) 

49.056 

(0.760) 

Average 67.592 

(9.789) 

68.771 

(9.785) 

68.253 

(9.779) 

68.686 

(9.782) 

67.571 

(9.782) 

Mating Behavior

Length of Time to First 

Neck Grab (sec) - Day 1 

Lab1_ST NA NA NA NA NA 

Lab2_SV 52.143 

(41.928) NA NA NA 50.429 (41.836) 

Lab3_WLI 48.286 

(42.004) 

17.286 

(10.146) 

18.429 

(8.693) 

90.143 

(54.217) 

93.286 

(53.616) 

Average 50.218 

(29.674) 

17.286 

(10.146) 

18.429 

(8.693) 

90.143 

(54.217) 

66.648 

(32.983) 

Length of Time to First 

Neck Grab (sec) - Day 2 

Lab1_ST NA NA NA NA NA 

Lab2_SV 44.714 

(42.549) NA NA NA 5.429 (2.975) 

Lab3_WLI 33.286 

(29.624) 

5.429 

(1.088) 

4.143 

(1.610) 

16.286 

(9.257) 

57.571 

(41.961) 

Average 37.017 

(24.312) 

5.429 

(1.088) 

4.143 

(1.610) 

16.286 

(9.257) 

5.689 

(2.968) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

101 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued)

Length of Time to First 

Neck Grab (sec) - Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV 44.857 

(42.525) NA NA NA 5.000 (2.854) 

Lab3_WLI 46.571 

(42.272) 

3.143 

(0.911) 

3.000 

(0.535) 

6.571 

(3.337) 

47.429 

(42.179) 

Average 45.719 

(29.980) 

3.143 

(0.911) 

3.000 

(0.535) 

6.571 

(3.337) 

5.193 

(2.847) 

Change in Length of Time 

to First Neck Grab (sec) 

from Day 1 to Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV -7.286 

(7.495) NA NA NA -45.429 (39.031) 

Lab3_WLI -1.714 

(2.427) 

-14.143 

(9.400) 

-15.429 

(8.476) 

-83.571 

(51.946) 

-45.857 

(39.189) 

Average -2.243 

(2.309) 

-14.143 

(9.400) 

-15.429 

(8.476) 

-83.571 

(51.946) 

-45.642 

(27.655) 

Length of Time to First 

Mount (sec) - Day 1 

Lab1_ST NA NA NA NA NA 

Lab2_SV 57.143 

(41.130) NA NA NA 85.857 (46.810) 

Lab3_WLI 54.286 

(41.221) 

31.286 

(18.026) 

20.429 

(8.488) 

90.857 

(54.030) 

94.143 

(53.377) 

Average 55.717 

(29.115) 

31.286 

(18.026) 

20.429 

(8.488) 

90.857 

(54.030) 

89.459 

(35.194) 

Length of Time to First 

Mount (sec) - Day 2 

Lab1_ST NA NA NA NA NA 

Lab2_SV 45.857 

(42.358) NA NA NA 59.429 (42.150) 

Lab3_WLI 89.286 

(54.415) 

7.857 

(1.805) 

8.286 

(1.539) 

18.571 

(9.021) 

60.857 

(42.040) 

Average 62.243 

(33.425) 

7.857 

(1.805) 

8.286 

(1.539) 

18.571 

(9.021) 

60.145 

(29.766) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

102 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued)

Length of Time to First 

Mount (sec) - Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV 45.571 

(42.406) NA NA NA 10.143 (4.228) 

Lab3_WLI 48.143 

(42.043) 

4.143 

(0.911) 

5.000 

(0.951) 

8.000 

(3.754) 

48.429 

(42.012) 

Average 46.868 

(29.856) 

4.143 

(0.911) 

5.000 

(0.951) 

8.000 

(3.754) 

10.527 

(4.207) 

Change in Length of Time 

to First Mount (sec) from 

Day 1 to Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV -11.571 

(7.662) NA NA NA -75.714 (46.082) 

Lab3_WLI -6.143 

(2.807) 

-27.143 

(17.305) 

-15.429 

(8.363) 

-82.857 

(51.499) 

-45.714 

(39.005) 

Average -6.785 

(2.635) 

-27.143 

(17.305) 

-15.429 

(8.363) 

-82.857 

(51.499) 

-58.236 

(29.772) 

Number of Mount 

Attempts (count) - Day 1 

Lab1_ST NA NA NA NA NA 

Lab2_SV 9.143 

(2.176) NA NA NA 10.714 (3.137) 

Lab3_WLI 3.286 

(0.778) 

4.143 

(0.911) 

6.857 

(1.682) 

5.429 

(1.478) 

3.286 

(1.107) 

Average 6.193 

(3.204) 

7.044 

(3.300) 

9.759 

(3.590) 

8.330 

(3.499) 

6.367 

(3.290) 

Number of Mount 

Attempts (count) - Day 2 

Lab1_ST NA NA NA NA NA 

Lab2_SV 11.571 

(3.184) NA NA NA 6.286 (2.135) 

Lab3_WLI 4.714 

(1.248) 

4.286 

(0.474) 

6.429 

(1.429) 

6.286 

(1.248) 

4.429 

(1.360) 

Average 6.540 

(1.997) 

5.529 

(1.785) 

7.672 

(2.237) 

7.529 

(2.126) 

5.490 

(1.923) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

103 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued)

Number of Mount 

Attempts (count) - Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV 8.714 

(1.948) NA NA NA 7.857 (1.625) 

Lab3_WLI 5.000 

(1.558) 

3.429 

(0.481) 

5.714 

(1.475) 

5.143 

(0.595) 

3.857 

(0.857) 

Average 6.816 

(2.181) 

5.098 

(1.989) 

7.384 

(2.429) 

6.812 

(2.019) 

5.670 

(1.995) 

Change in Number of 

Mount Attempts (count) 

from Day 1 to Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV -0.429 

(2.298) NA NA NA -2.857 (2.632) 

Lab3_WLI 1.714 

(1.672) 

-0.714 

(1.017) 

-1.143 

(1.765) 

-0.286 

(1.714) 

0.571 

(0.812) 

Average 0.755 

(1.693) 

-1.420 

(1.586) 

-1.849 

(2.144) 

-0.992 

(2.103) 

-0.310 

(1.389) 

Number of Cloacal 

Contacts (count) - Day 1 

Lab1_ST NA NA NA NA NA 

Lab2_SV 3.143 

(0.829) NA NA NA 2.571 (0.649) 

Lab3_WLI 2.000 

(0.787) 

2.857 

(0.911) 

3.429 

(0.922) 

2.143 

(0.800) 

2.143 

(0.670) 

Average 2.542 

(0.571) 

2.857 

(0.911) 

3.429 

(0.922) 

2.143 

(0.800) 

2.364 

(0.466) 

Number of Cloacal 

Contacts (count) - Day 2 

Lab1_ST NA NA NA NA NA 

Lab2_SV 5.143 

(1.487) NA NA NA 2.857 (0.670) 

Lab3_WLI 2.429 

(0.751) 

2.429 

(0.481) 

3.000 

(0.488) 

2.429 

(0.429) 

1.571 

(0.571) 

Average 3.342 

(0.988) 

3.038 

(0.913) 

3.609 

(0.917) 

3.038 

(0.886) 

2.209 

(0.824) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

104 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued)

Number of Cloacal 

Contacts (count) - Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV 4.429 

(0.972) NA NA NA 4.571 (0.782) 

Lab3_WLI 2.286 

(0.522) 

2.714 

(0.522) 

2.286 

(0.286) 

2.143 

(0.705) 

2.000 

(0.436) 

Average 3.373 

(1.251) 

3.814 

(1.305) 

3.386 

(1.230) 

3.243 

(1.388) 

3.188 

(1.223) 

Change in Number of 

Cloacal Contacts (count) 

from Day 1 to Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV 1.286 

(0.421) NA NA NA 2.000 (0.690) 

Lab3_WLI 0.286 

(0.778) 

-0.143 

(1.100) 

-1.143 

(0.829) 

0.000 

(1.175) 

-0.143 

(0.800) 

Average 0.742 

(0.768) 

0.436 

(1.314) 

-0.564 

(1.097) 

0.579 

(1.378) 

1.001 

(0.837) 

Number of Crows (Cock) 

or Fluffs (Hen) (count) - 

Day 1 

Lab1_ST NA NA NA NA NA 

Lab2_SV 6.857 

(2.262) NA NA NA 5.857 (1.534) 

Lab3_WLI 1.000 

(0.309) 

0.714 

(0.360) 

0.857 

(0.459) 

0.714 

(0.360) 

0.571 

(0.202) 

Average 3.595 

(2.741) 

3.297 

(2.752) 

3.440 

(2.767) 

3.297 

(2.752) 

3.156 

(2.731) 

Number of Crows (Cock) 

or Fluffs (Hen) (count) - 

Day 2 

Lab1_ST NA NA NA NA NA 

Lab2_SV 6.000 

(0.951) NA NA NA 4.143 (1.100) 

Lab3_WLI 0.714 

(0.421) 

1.286 

(0.421) 

2.000 

(1.024) 

0.143 

(0.143) 

0.286 

(0.286) 

Average 3.095 

(2.332) 

3.540 

(2.354) 

4.254 

(2.533) 

2.397 

(2.321) 

2.499 

(2.326) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

105 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Mating Behavior (Continued)

Number of Crows (Cock) 

or Fluffs (Hen) (count) - 

Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV 4.714 

(0.865) NA NA NA 6.857 (1.654) 

Lab3_WLI 1.286 

(0.522) 

1.286 

(0.474) 

1.714 

(0.522) 

0.857 

(0.459) 

1.000 

(0.488) 

Average 3.081 

(2.093) 

3.239 

(2.166) 

3.667 

(2.177) 

2.810 

(2.163) 

3.202 

(2.133) 

Change in Number of 

Crows (Cock) or Fluffs 

(Hen) (count) from Day 1 

to Day 3 

Lab1_ST NA NA NA NA NA 

Lab2_SV -2.143 

(2.632) NA NA NA 1.000 (2.289) 

Lab3_WLI 0.286 

(0.360) 

0.571 

(0.612) 

0.857 

(0.459) 

0.143 

(0.508) 

0.429 

(0.481) 

Average 0.241 

(0.356) 

0.571 

(0.612) 

0.857 

(0.459) 

0.143 

(0.508) 

0.453 

(0.471) 

Thyroid Weight

14-Day Old Left Thyroid 

Weight (mg) 

Lab1_ST 4.794 

(0.239) 

4.427 

(0.333) 

4.494 

(0.338) 

4.571 

(0.349) 

5.164 

(0.497) 

Lab2_SV NA NA NA NA NA 

Lab3_WLI NA NA NA NA NA 

Average NA NA NA NA NA 

14-Day Old Left Thyroid 

Weight (mg) - Males 

Lab1_ST NA NA NA NA NA 

Lab2_SV 4.029 

(0.344) 

3.242 

(0.365) 

2.379 

(0.310) 

3.610 

(0.414) 

2.893 

(0.355) 

Lab3_WLI 1.913 

(0.218) 

2.800 

(0.506) 

3.347 

(0.278) 

2.706 

(0.272) 

2.333 

(0.245) 

Average 2.917 

(0.587) 

3.032 

(0.631) 

2.871 

(0.589) 

3.132 

(0.603) 

2.604 

(0.591) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

106 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Thyroid Weight (Continued)

14-Day Old Left Thyroid 

Weight (mg) - Females 

Lab1_ST NA NA NA NA NA 

Lab2_SV 2.567 

(0.370) 

3.207 

(0.436) 

5.231 

(0.561) 

3.529 

(0.406) 

3.383 

(0.647) 

Lab3_WLI 2.080 

(0.248) 

2.327 

(0.342) 

2.907 

(0.348) 

2.688 

(0.391) 

2.217 

(0.287) 

Average 2.384 

(0.557) 

2.773 

(0.582) 

3.814 

(0.599) 

3.110 

(0.587) 

2.737 

(0.590) 

15 Day Embryo (ED15) 

Thyroid Weight (mg) 

Lab1_ST 1.106 

(0.088) 

1.147 

(0.076) 

1.169 

(0.069) 

1.350 

(0.112) 

1.271 

(0.128) 

Lab2_SV 1.850 

(0.216) 

1.927 

(0.161) 

2.092 

(0.194) 

2.096 

(0.229) 

2.142 

(0.211) 

Lab3_WLI 3.286 

(0.324) 

3.300 

(0.168) 

3.479 

(0.187) 

3.538 

(0.164) 

3.606 

(0.162) 

Average 2.108 

(0.658) 

2.145 

(0.656) 

2.203 

(0.656) 

2.343 

(0.658) 

2.329 

(0.659) 

Sex Ratio

Ratio of Number of Males 

to Total Number of 

Offspring (%) 

Lab1_ST 51.580 

(3.791) 

45.399 

(4.353) 

50.768 

(4.339) 

48.791 

(4.954) 

42.099 

(4.724) 

Lab2_SV 53.701 

(4.432) 

52.017 

(4.270) 

45.566 

(4.030) 

53.537 

(4.257) 

52.948 

(2.906) 

Lab3_WLI 41.527 

(4.203) 

43.655 

(4.523) 

49.628 

(4.908) 

42.121 

(4.285) 

52.789 

(4.093) 

Average 49.000 

(2.973) 

47.104 

(3.091) 

48.354 

(3.100) 

48.102 

(3.137) 

50.085 

(2.819) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

107 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Bio Chem

Plasma Thyroid Hormone 

T3 - 14-Day Old Males 

(ng/mL) 

Lab1_ST 0.769 

(0.061) 

0.648 

(0.063) 

0.736 

(0.095) 

0.969 

(0.203) 

0.521 

(0.107) 

Lab2_SV 0.572 

(0.092) 

1.545 

(0.173) 

0.990 

(0.320) 

1.389 

(0.279) 

1.005 

(0.286) 

Lab3_WLI 1.503 

(0.114) 

1.775 

(0.224) 

1.570 

(0.242) 

1.594 

(0.173) 

1.972 

(0.141) 

Average 0.952 

(0.315) 

1.293 

(0.323) 

1.111 

(0.334) 

1.295 

(0.335) 

1.170 

(0.327) 

Plasma Thyroid Hormone 

T3 - 14-Day Old Females 

(ng/mL) 

Lab1_ST 0.817 

(0.241) 

0.869 

(0.223) 

0.757 

(0.094) 

0.756 

(0.087) 

0.585 

(0.018) 

Lab2_SV 1.232 

(0.159) 

1.026 

(0.169) 

1.151 

(0.309) 

1.673 

(0.253) 

1.185 

(0.252) 

Lab3_WLI 2.333 

(0.551) 

2.155 

(0.195) 

1.657 

(0.249) 

2.051 

(0.185) 

1.970 

(0.231) 

Average 1.371 

(0.397) 

1.321 

(0.388) 

1.276 

(0.385) 

1.390 

(0.382) 

1.175 

(0.379) 

Plasma Thyroid Hormone 

T4 - 14-Day Old Males 

(ng/mL) 

Lab1_ST 4.082 

(0.833) 

5.387 

(1.570) 

3.642 

(1.370) 

3.238 

(1.402) 

2.111 

(0.383) 

Lab2_SV 2.989 

(1.293) 

1.721 

(0.597) 

5.640 

(1.424) 

1.734 

(0.794) 

3.352 

(1.503) 

Lab3_WLI 1.215 

(0.292) 

3.036 

(0.842) 

1.251 

(0.283) 

2.743 

(0.920) 

1.330 

(0.348) 

Average 2.563 

(0.840) 

2.949 

(0.892) 

2.872 

(0.899) 

2.506 

(0.917) 

1.977 

(0.813) 

Plasma Thyroid Hormone 

T4 - 14-Day Old Females 

(ng/mL) 

Lab1_ST 3.760 

(1.076) 

5.013 

(1.038) 

5.022 

(1.694) 

4.427 

(0.933) 

2.305 

(0.697) 

Lab2_SV 2.660 

(1.029) 

2.782 

(0.764) 

2.300 

(1.354) 

1.429 

(0.497) 

3.422 

(0.861) 

Lab3_WLI 2.449 

(0.845) 

1.231 

(0.296) 

1.041 

(0.278) 

0.896 

(0.133) 

1.775 

(0.488) 

Average 3.016 

(0.910) 

2.670 

(0.806) 

2.270 

(0.858) 

1.986 

(0.771) 

2.470 

(0.812) 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

108 

Table 16. (Continued). Mean and Standard Errors and Comparisons of Avian Vinclozolin 

Concentrations to Control across Laboratories, F2 Generationf 

Parameter Laboratory 

Mean Standard Errora,b

Concentration 

Trend Testsc

Overall 

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Significant 

Concentration 

Effectd,e

Bio Chem (Continued)

Plasma Sex Hormone 

Estradiol - 14-Day Old 

Males (pg/mL) 

Lab1_ST 2.884 

(0.287) 

2.153 

(0.432) 

2.522 

(0.528) 

3.573 

(1.216) 

1.588 

(0.338) 

Lab2_SV 5.747 

(1.701) 

7.748 

(1.842) 

6.110 

(1.220) 

9.061 

(3.703) 

6.298 

(1.498) 

Lab3_WLI 4.918 

(1.218) 

3.700 

(0.000) 

3.700 

(0.000) 

3.700 

(0.000) 

3.700 

(0.000) 

Average 4.724 

(1.242) 

3.969 

(1.211) 

3.989 

(1.210) 

3.986 

(1.216) 

3.858 

(1.209) 

Plasma Sex Hormone 

Estradiol - 14-Day Old 

Females (pg/mL) 

Lab1_ST 12.116 

(1.552) 

8.990 

(1.180) 

11.509 

(1.465) 

7.871 

(0.835) 

3.273 

(0.874) 

Lab2_SV 9.570 

(2.030) 

8.584 

(2.945) 

14.955 

(3.556) 

21.943 

(4.226) 

10.732 

(1.834) 

Lab3_WLI 14.803 

(2.333) 

9.955 

(1.868) 

12.423 

(3.203) 

10.396 

(1.730) 

11.789 

(2.277) 

Average 12.165 

(2.033) 

9.427 

(2.030) 

12.869 

(2.262) 

11.094 

(2.060) 

7.998 

(1.943) 

Plasma Sex Hormone 

Testosterone - 14-Day Old 

Males (pg/mL) 

Lab1_ST 46.288 

(11.184) 

23.588 

(11.528) 

37.586 

(10.607) 

23.477 

(5.454) 

26.986 

(8.186) 

Lab2_SV 6.625 

(1.167) 

3.900 

(0.000) 

9.161 

(3.077) 

6.878 

(1.941) 

6.702 

(1.859) 

Lab3_WLI 24.139 

(4.080) 

27.230 

(7.393) 

27.104 

(2.268) 

28.495 

(9.851) 

35.290 

(12.830) 

Average 8.331 

(1.116) 

3.900 

(0.000) 

21.273 

(1.799) 

9.402 

(1.798) 

8.237 

(1.795) Yes 

Plasma Sex Hormone 

Testosterone - 14-Day Old 

Females (pg/mL) 

Lab1_ST 26.237 

(4.314) 

20.436 

(6.196) 

32.224 

(2.784) 

26.117 

(2.732) 

24.556 

(3.530) 

Lab2_SV 4.680 

(0.780) 

3.900 

(0.000) 

4.965 

(1.065) 

8.351 

(3.310) 

6.195 

(2.295) 

Lab3_WLI 31.051 

(13.215) 

38.684 

(14.877) 

33.448 

(3.541) 

29.657 

(5.000) 

23.100 

(2.804) 

Average 5.449 

(0.766) 

3.900 

(0.000) 

10.269 

(0.957) 

20.537 

(1.942) 

15.315 

(1.587) 

An increasing trend 

for the highest three 

concentration 

groups 

Yes 

a. Means and their associated standard errors for each concentration group within each laboratory were pooled across all samples within the 

concentration group within the laboratory. 

b. Averages and standard errors across laboratory for each concentration group were based on random effects model with concentration group 

treated as a fixed effect and laboratory and interaction of laboratory and concentration group treated as random effects, incorporating 

heterogeneous variances of group within laboratory. 

c. Step-down trend tests were performed. 

d. The overall concentration effects were determined by the above random effect model. The degrees of freedom for the fixed concentration 

effect were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the total random variance, Si

2

 and dfi are the 

reported variance and degrees of freedom for laboratory i, var(SR

2

) is the variance of SR

2

, and K is the number of studies (Hartung and 

Makambi, 2001) 

e. If concentration effect is significant, then pairwise comparisons of test concentrations to the control were performed using both unadjusted 

two-sample t-tests and the Dunnett’s tests. Concentration groups with significant difference from the controls were listed and marked with 

“*”if detected using the unadjusted t-tests and with “**” if detected using the Dunnett’s tests. 

f. “NA” indicates that data were not available. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

109 

Table 17. Differences between Generations across Laboratories. Chemical Vincolozolin, Avian 

Studya,b. 

Parameter 

Difference 

Between 

Generations 

Difference of Averages Between Generations 

(Standard Error)c

Concentration 

Trend Testsd

Groups with 

Significant 

Difference 

between 

Generationse,f

Control 

(Group 1) 

64 ppm 

(Group 2)

160 ppm 

(Group 3) 

400 ppm 

(Group 4)

1000 ppm 

(Group 5) 

Body Weight

Adult Body Weight (g) Day 0 - 

Males F1-F0 -31.514 (10.793) 

-28.926 

(10.814) 

-32.090 

(10.814) 

-32.218 

(10.833) 

-30.462 

(10.829) 

Adult Body Weight (g) Day 56 - 

Males F1-F0 -3.652 (2.730) 

3.492 

(3.206) 

0.699 

(3.068) 

2.985 

(3.024) 

0.049 

(2.494) 

Adult Body Weight (g) Day 0 - 

Females F1-F0 -58.260 (9.394) 

-58.115 

(9.139) 

-60.846 

(9.171) 

-63.089 

(9.099) 

-54.244 

(9.099) All 5 Groups ()

Adult Body Weight (g) Day 56 - 

Females F1-F0 1.534 (4.392) 

3.506 

(4.000) 

-1.073 

(4.119) 

1.652 

(4.745) 

3.770 

(4.474) 

Mean Offspring Body Weight by 

Pen (g) Day 0 F2-F1 -0.293 (0.151) 

-0.193 

(0.133) 

-0.499 

(0.146) 

-0.385 

(0.132) 

-0.320 

(0.137) 

Mean Offspring Body Weight by 

Pen (g) Day 14 F2-F1 -2.683 (3.503) 

-1.104 

(3.507) 

-2.812 

(3.483) 

-1.498 

(3.460) 

-3.414 

(3.490) 

Feed Consumption

Adult Feed Consumption 

(g/bird/day) - Week 1 F1-F0 0.810 (1.087) 

1.563 

(1.140) 

2.211 

(1.203) 

0.963 

(1.117) 

2.251 

(1.102) 

Adult Feed Consumption 

(g/bird/day) - Week 2 F1-F0 3.466 (2.614) 

2.201 

(2.636) 

4.303 

(2.616) 

5.241 

(2.608) 

4.411 

(2.633) 

Adult Feed Consumption 

(g/bird/day) - Week 3 F1-F0 3.627 (1.216) 

2.715 

(1.207) 

3.373 

(1.235) 

3.053 

(1.184) 

4.019 

(1.240) 

Adult Feed Consumption 

(g/bird/day) - Week 4 F1-F0 0.026 (1.630) 

-1.820 

(1.630) 

-1.015 

(1.624) 

-0.726 

(1.614) 

-0.393 

(1.626) 

Adult Feed Consumption 

(g/bird/day) - Week 5 F1-F0 -2.413 (1.358) 

-0.843 

(1.330) 

-1.672 

(1.341) 

-0.442 

(1.290) 

-0.316 

(1.316) 

Adult Feed Consumption 

(g/bird/day) - Week 6 F1-F0 -1.227 (1.355) 

-0.263 

(1.386) 

-0.391 

(1.360) 

1.271 

(1.347) 

1.062 

(1.365) 

a. random effects model with generation, concentration group and interaction of generation and concentration group treated as fixed 

effect, and laboratory, laboratory by generation and laboratory by generation by group as random effects, incorporating heterogeneous 

variances of group within laboratory and generation. 

b. The degrees of freedom for the fixed effects were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the 

total random variance across laboratories, Si

2

 and dfi are the reported variance and degrees of freedom within laboratory i, var(SR

2

) is 

the variance of SR

2

, and K is the number of studies (Hartung and Makambi, 2001) 

c. Differences of averages (least square means) between two generations and standard errors across laboratory for each concentration 

group were based on the above random effect models. 

d. Step-down trend tests were performed in the differences between the two generations. 

e. If generation or the interaction of generation and concentration group effect was significant, then F-tests were used to identify the 

concentration groups in which the two generations are significantly different at the 0.05 level. 

f. “↓” F2 or F1 generation less than F1or F0 generation. 

“↑” F2 or F1 generation greater than F1or F0 generation. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

110 

Table 17. (Continued). Differences between Generations across Laboratories. Chemical 

Vincolozolin, Avian Studya,b.

Parameter 

Difference 

Between 

Generations 

Difference of Averages Between Generations 

(Standard Error)c

Concentration 

Trend Testsd

Groups with 

Significant 

Difference 

between 

Generationse,f

Control 

(Group 1) 

64 ppm 

(Group 2)

160 ppm 

(Group 3) 

400 ppm 

(Group 4)

1000 ppm 

(Group 5) 

Feed Consumption (Continued)

Adult Feed Consumption 

(g/bird/day) - Week 7 F1-F0 -0.135 (1.660) 

-0.290 

(1.730) 

-1.251 

(1.708) 

0.341 

(1.701) 

1.450 

(1.681) 

Adult Feed Consumption 

(g/bird/day) - Week 8 F1-F0 -1.253 (1.055) 

-0.266 

(1.087) 

-1.789 

(1.094) 

-0.508 

(1.125) 

-0.445 

(1.100) 

Adult Feed Consumption 

(g/bird/day) - Week 9 F1-F0 -2.857 (1.853) 

-3.211 

(1.982) 

-5.917 

(1.803) 

-1.293 

(1.838) 

4.675 

(1.780) 

Reproduction

Eggs Laid F1-F0 0.442 

(2.296) 

0.182 

(2.479) 

1.892 

(2.642) 

-0.328 

(2.312) 

2.552 

(2.277) 

Eggs Crack F1-F0 0.392 

(0.295) 

0.520 

(0.227) 

0.255 

(0.223) 

0.070 

(0.266) 

0.200 

(0.220) 

Eggs Abnormal F1-F0 0.317 

(0.557) 

0.336 

(0.547) 

0.360 

(0.528) 

0.331 

(0.509) 

0.677 

(0.545) 

Eggs Set From Weeks 7 and 8 F1-F0 -0.129 

(0.496) 

0.241 

(0.593) 

-0.126 

(0.571) 

-0.051 

(0.503) 

0.156 

(0.568) 

Eggs Set From Weeks 6 7 and 8 F1-F0 6.664 

(7.231) 

7.566 

(7.241) 

7.517 

(7.246) 

7.081 

(7.231) 

7.835 

(7.243) 

Eggs Fertile From Weeks 7 and 

8 F1-F0 -0.402 (0.478) 

-0.421 

(0.493) 

0.048 

(0.517) 

-0.618 

(0.505) 

0.225 

(0.487) 

Eggs Fertile From Weeks 6 7 

and 8 F1-F0 6.089 (6.902) 

7.172 

(6.915) 

7.328 

(6.920) 

5.507 

(6.921) 

6.851 

(6.914) 

Eggs Viable From Weeks 7 and 

8 F1-F0 1.405 (2.091) 

1.583 

(2.091) 

2.235 

(2.099) 

1.237 

(2.121) 

2.096 

(2.100) 

Eggs Viable From Weeks 6 7 

and 8 F1-F0 5.785 (6.239) 

6.484 

(6.251) 

7.693 

(6.244) 

4.988 

(6.278) 

6.179 

(6.257) 

a. random effects model with generation, concentration group and interaction of generation and concentration group treated as fixed 

effect, and laboratory, laboratory by generation and laboratory by generation by group as random effects, incorporating heterogeneous 

variances of group within laboratory and generation. 

b. The degrees of freedom for the fixed effects were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the 

total random variance across laboratories, Si

2

 and dfi are the reported variance and degrees of freedom within laboratory i, var(SR

2

) is 

the variance of SR

2

, and K is the number of studies (Hartung and Makambi, 2001) 

c. Differences of averages (least square means) between two generations and standard errors across laboratory for each concentration 

group were based on the above random effect models. 

d. Step-down trend tests were performed in the differences between the two generations. 

e. If generation or the interaction of generation and concentration group effect was significant, then F-tests were used to identify the 

concentration groups in which the two generations are significantly different at the 0.05 level. 

f. “↓” F2 or F1 generation less than F1or F0 generation. 

“↑” F2 or F1 generation greater than F1or F0 generation. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

111 

Table 17. (Continued). Differences between Generations across Laboratories. Chemical 

Vincolozolin, Avian Studya,b.

Parameter 

Difference 

Between 

Generations 

Difference of Averages Between Generations 

(Standard Error)c

Concentration 

Trend Testsd

Groups with 

Significant 

Difference 

between 

Generationse,f

Control 

(Group 1)

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4)

1000 ppm 

(Group 5) 

Reproduction (Continued)

Viable Eggs From Weeks 7 and 8 

after Two Weeks of Incubation F1-F0 1.339 (2.074) 

1.164 

(2.083) 

2.188 

(2.089) 

1.313 

(2.101) 

2.082 

(2.083) 

Viable Eggs From Weeks 6 7 and 

8 after Two Weeks of Incubati F1-F0 -0.644 (0.746) 

-0.075 

(0.841) 

0.470 

(0.863) 

-0.829 

(0.939) 

0.596 

(0.846) 

Eggs Hatched F1-F0 -0.013 

(0.630) 

-0.826 

(0.688) 

0.422 

(0.698) 

-0.051 

(0.686) 

0.190 

(0.664) 

Number of 14-Day-Old Surviving 

Chicks F1-F0 1.059 (0.928) 

-0.126 

(0.958) 

0.944 

(0.930) 

0.348 

(0.948) 

0.940 

(0.948) 

Eggs Laid Per Hen Per Day F1-F0 -0.001 

(0.047) 

0.014 

(0.049) 

0.054 

(0.055) 

-0.002 

(0.047) 

0.053 

(0.047) 

Cracked Eggs as A Percentage of 

Eggs Laid F1-F0 0.007 (0.007) 

0.011 

(0.008) 

0.004 

(0.006) 

0.002 

(0.007) 

0.002 

(0.006) 

Abnormal Eggs as A Percentage of 

Eggs Laid F1-F0 0.008 (0.014) 

0.004 

(0.015) 

0.009 

(0.014) 

0.008 

(0.013) 

0.016 

(0.014) 

Fertile Eggs as A Percentage of 

Eggs Set From Weeks 7 and 8 F1-F0 0.002 (0.013) 

-0.003 

(0.015) 

0.031 

(0.022) 

-0.017 

(0.030) 

0.023 

(0.012) 

Fertile Eggs as A Percentage of 

Eggs Set From Weeks 6 7 and F1-F0 -0.015 (0.015) 

0.012 

(0.019) 

0.022 

(0.023) 

-0.016 

(0.027) 

0.001 

(0.014) 

Viable Eggs as A Percentage of 

Eggs Set From Weeks 7 and 8 F1-F0 -0.012 (0.022) 

0.002 

(0.023) 

0.047 

(0.025) 

-0.020 

(0.043) 

0.016 

(0.023) 

Viable Eggs as A Percentage of 

Eggs Set From Weeks 6 7 and 8 F1-F0 -0.015 (0.028) 

0.004 

(0.030) 

0.030 

(0.031) 

-0.040 

(0.046) 

-0.025 

(0.028) 

Viable Eggs from Weeks 7 and 8 

after Two Weeks of Incubation F1-F0 -0.001 (0.005) 

-0.004 

(0.005) 

0.000 

(0.006) 

-0.003 

(0.005) 

-0.001 

(0.004) 

a. random effects model with generation, concentration group and interaction of generation and concentration group treated as fixed 

effect, and laboratory, laboratory by generation and laboratory by generation by group as random effects, incorporating heterogeneous 

variances of group within laboratory and generation. 

b. The degrees of freedom for the fixed effects were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the 

total random variance across laboratories, Si

2

 and dfi are the reported variance and degrees of freedom within laboratory i, var(SR

2

) is 

the variance of SR

2

, and K is the number of studies (Hartung and Makambi, 2001) 

c. Differences of averages (least square means) between two generations and standard errors across laboratory for each concentration 

group were based on the above random effect models. 

d. Step-down trend tests were performed in the differences between the two generations. 

e. If generation or the interaction of generation and concentration group effect was significant, then F-tests were used to identify the 

concentration groups in which the two generations are significantly different at the 0.05 level. 

f. “↓” F2 or F1 generation less than F1or F0 generation. 

“↑” F2 or F1 generation greater than F1or F0 generation. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

112 

Table 17. (Continued). Differences between Generations across Laboratories. Chemical 

Vincolozolin, Avian Studya,b.

Parameter 

Difference 

Between 

Generations 

Difference of Averages Between Generations 

(Standard Error)c

Concentration 

Trend Testsd

Groups with 

Significant 

Difference 

between 

Generationse,f

Control 

(Group 1)

64 ppm 

(Group 2) 

160 ppm 

(Group 3)

400 ppm 

(Group 4)

1000 ppm 

(Group 5) 

Reproduction (Continued)

Viable Eggs from Weeks 6 7 and 8 

after Two Weeks of Incubation F1-F0 0.000 (0.004) 

-0.004 

(0.005) 

-0.000 

(0.005) 

-0.002 

(0.004) 

-0.001 

(0.004) 

Eggs Hatched as A Percentage of 

Number of Viable Eggs from W F1-F0 0.023 (0.017) 

-0.004 

(0.020) 

0.002 

(0.011) 

0.011 

(0.020) 

-0.011 

(0.017) 

Number of 14-Day-Old Surviving 

Chicks as A Percentage of Egg F1-F0 0.073 (0.065) 

0.068 

(0.065) 

0.087 

(0.066) 

0.054 

(0.066) 

0.052 

(0.065) 

Eggs Hatched as A Percentage of 

Number of Eggs Set from Week F1-F0 0.008 (0.045) 

-0.025 

(0.047) 

0.057 

(0.047) 

0.012 

(0.055) 

-0.002 

(0.045) 

Eggs Hatched as A Percentage of 

Number of Fertile Eggs from F1-F0 0.018 (0.036) 

-0.028 

(0.038) 

0.064 

(0.039) 

0.013 

(0.043) 

-0.012 

(0.037) 

Number of 14-Day-Old Surviving 

Chicks as A Percentage of Num F1-F0 0.087 (0.069) 

0.031 

(0.069) 

0.130 

(0.070) 

0.047 

(0.073) 

0.054 

(0.068) 

Eggs Hatched Per Hen Per Day F1-F0 0.002 

(0.049) 

-0.055 

(0.053) 

0.072 

(0.051) 

0.000 

(0.053) 

0.015 

(0.051) 

Number of 14-Day-Old Surviving 

Chicks Per Hen Per Day F1-F0 0.073 (0.066) 

-0.006 

(0.068) 

0.113 

(0.064) 

0.025 

(0.068) 

0.058 

(0.068) 

Egg Shell and Weight

Pen Mean Egg Shell Thickness 

(mm) F1-F0 -0.002 (0.004) 

0.001 

(0.004) 

0.001 

(0.004) 

-0.002 

(0.004) 

0.003 

(0.004) 

Pen Mean Egg Content Weight - 

Albumin (g) F1-F0 0.572 (0.296) 

0.894 

(0.301) 

0.774 

(0.296) 

0.683 

(0.291) 

0.873 

(0.287) 

Pen Mean Egg Content Weight - 

Yolk (g) F1-F0 -0.004 (0.137) 

-0.063 

(0.136) 

-0.058 

(0.139) 

0.050 

(0.130) 

-0.054 

(0.132) 

Pen Mean Egg Content Weight - 

Combined (g) F1-F0 0.637 (0.335) 

0.831 

(0.339) 

0.767 

(0.330) 

0.734 

(0.322) 

0.807 

(0.320) 

a. random effects model with generation, concentration group and interaction of generation and concentration group treated as fixed 

effect, and laboratory, laboratory by generation and laboratory by generation by group as random effects, incorporating heterogeneous 

variances of group within laboratory and generation. 

b. The degrees of freedom for the fixed effects were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the 

total random variance across laboratories, Si

2

 and dfi are the reported variance and degrees of freedom within laboratory i, var(SR

2

) is 

the variance of SR

2

, and K is the number of studies (Hartung and Makambi, 2001) 

c. Differences of averages (least square means) between two generations and standard errors across laboratory for each concentration 

group were based on the above random effect models. 

d. Step-down trend tests were performed in the differences between the two generations. 

e. If generation or the interaction of generation and concentration group effect was significant, then F-tests were used to identify the 

concentration groups in which the two generations are significantly different at the 0.05 level. 

f. “↓” F2 or F1 generation less than F1or F0 generation. 

“↑” F2 or F1 generation greater than F1or F0 generation. 

 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

113 

Table 17. (Continued). Differences between Generations across Laboratories. Chemical 

Vincolozolin, Avian Studya,b.

Parameter 

Difference 

Between 

Generations 

Difference of Averages Between Generations 

(Standard Error)c

Concentration 

Trend Testsd

Groups with 

Significant 

Difference 

between 

Generationse,f

Control 

(Group 1) 

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4) 

1000 ppm 

(Group 5) 

Mating Behavior

Length of Time to First Neck 

Grab (sec) - Day 1 F2-F1 36.342 (30.667) 

12.022 

(13.878) 

1.874 

(12.819) 

82.456 

(55.040) 

54.568 

(33.913) 

Length of Time to First Neck 

Grab (sec) - Day 2 F2-F1 30.486 (24.365) 

3.114 

(2.097) 

-1.145 

(2.401) 

11.981 

(9.466) 

-2.688 

(3.949) 

Length of Time to First Neck 

Grab (sec) - Day 3 F2-F1 42.601 (29.981) 

0.100 

(1.213) 

-0.253 

(0.627) 

4.394 

(3.355) 

1.861 

(2.869) 

Change in Length of Time to 

First Neck Grab (sec) from 

Day 1 to Day 3 

F2-F1 3.266 

(3.665) 

-11.070 

(9.850) 

-8.356 

(9.033) 

-78.997 

(52.025) 

-42.291 

(27.806) 

Decreasing in 

difference (F2-

F1) at the highest 

concentration 

Length of Time to First 

Mount (sec) - Day 1 F2-F1 10.095 (34.054) 

20.207 

(22.197) 

-0.804 

(15.332) 

59.161 

(55.994) 

68.355 

(36.974) 

Length of Time to First 

Mount (sec) - Day 2 F2-F1 47.892 (33.623) 

4.080 

(2.128) 

-0.724 

(2.149) 

6.923 

(9.407) 

48.599 

(29.832) 

Length of Time to First 

Mount (sec) - Day 3 F2-F1 41.612 (29.866) 

0.244 

(1.288) 

0.241 

(1.032) 

5.317 

(3.802) 

4.821 

(4.212) . 

Change in Length of Time to 

First Mount (sec) from Day 1 

to Day 3 

F2-F1 17.219 

(13.434) 

-17.488 

(17.748) 

-2.243 

(10.231) 

-74.352 

(51.572) 

-47.936 

(30.084) 

Decreasing in 

difference (F2-

F1) at the highest 

concentration 

Number of Mount Attempts 

(count) - Day 1 F2-F1 -0.095 (2.454) 

0.375 

(2.571) 

2.765 

(2.963) 

2.982 

(2.835) 

-1.159 

(2.674) 

Number of Mount Attempts 

(count) - Day 2 F2-F1 0.674 (2.339) 

-0.199 

(2.204) 

1.983 

(2.585) 

1.606 

(2.519) 

-0.154 

(2.091) 

Number of Mount Attempts 

(count) - Day 3 F2-F1 -0.576 (1.724) 

-0.633 

(1.305) 

0.526 

(1.837) 

1.028 

(1.264) 

-1.317 

(1.353) 

Change in Number of Mount 

Attempts (count) from Day 1 

to Day 3 

F2-F1 -1.076 

(3.418) 

-1.210 

(3.465) 

-3.125 

(3.787) 

-2.677 

(3.800) 

-0.066 

(3.310) 

a. random effects model with generation, concentration group and interaction of generation and concentration group treated as fixed 

effect, and laboratory, laboratory by generation and laboratory by generation by group as random effects, incorporating heterogeneous 

variances of group within laboratory and generation. 

b. The degrees of freedom for the fixed effects were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the 

total random variance across laboratories, Si

2

 and dfi are the reported variance and degrees of freedom within laboratory i, var(SR

2

) is 

the variance of SR

2

, and K is the number of studies (Hartung and Makambi, 2001) 

c. Differences of averages (least square means) between two generations and standard errors across laboratory for each concentration 

group were based on the above random effect models. 

d. Step-down trend tests were performed in the differences between the two generations. 

e. If generation or the interaction of generation and concentration group effect was significant, then F-tests were used to identify the 

concentration groups in which the two generations are significantly different at the 0.05 level. 

f. “↓” F2 or F1 generation less than F1or F0 generation. 

“↑” F2 or F1 generation greater than F1or F0 generation. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

114 

Table 17. (Continued). Differences between Generations across Laboratories. Chemical 

Vincolozolin, Avian Studya,b.

Parameter 

Difference 

Between 

Generations 

Difference of Averages Between Generations 

(Standard Error)c

Concentration 

Trend Testsd

Groups with 

Significant 

Difference 

between 

Generationse,f

Control 

(Group 1)

64 ppm 

(Group 2) 

160 ppm 

(Group 3) 

400 ppm 

(Group 4)

1000 ppm 

(Group 5) 

Mating Behavior (Continued)

Number of Cloacal Contacts 

(count) - Day 1 F2-F1 -0.179 (0.700) 

0.734 

(0.975) 

0.853 

(0.970) 

-0.255 

(0.905) 

-0.926 

(0.731) 

Number of Cloacal Contacts 

(count) - Day 2 F2-F1 0.194 (0.891) 

-1.128 

(0.699) 

-0.259 

(0.620) 

-0.237 

(0.632) 

-0.915 

(0.519) 

Number of Cloacal Contacts 

(count) - Day 3 F2-F1 -0.811 (0.616) 

0.131 

(0.730) 

-1.157 

(0.452) 

-0.732 

(0.851) 

-1.301 

(0.561) 

Change in Number of Cloacal 

Contacts (count) from Day 1 to 

Day 3 

F2-F1 -0.539 

(0.926) 

-0.607 

(1.422) 

-1.791 

(1.212) 

-0.595 

(1.476) 

-0.223 

(0.993) 

Number of Crows (Cock) or 

Fluffs (Hen) (count) - Day 1 F2-F1 -0.910 (1.509) 

-0.172 

(1.714) 

-0.674 

(1.755) 

-0.193 

(1.674) 

-0.066 

(1.431) 

Number of Crows (Cock) or 

Fluffs (Hen) (count) - Day 2 F2-F1 0.355 (0.650) 

0.573 

(0.659) 

0.507 

(1.216) 

-0.167 

(0.623) 

-0.590 

(0.635) 

Number of Crows (Cock) or 

Fluffs (Hen) (count) - Day 3 F2-F1 -0.700 (1.389) 

-1.127 

(1.557) 

-1.091 

(1.504) 

-1.089 

(1.512) 

-1.855 

(1.452) 

Change in Number of Crows 

(Cock) or Fluffs (Hen) (count) fro F2-F1 0.666 (2.398) 

-0.872 

(2.583) 

-0.589 

(2.560) 

-0.565 

(2.543) 

-1.187 

(2.372) 

Thyroid Weight

15 Day Embryo (ED15) Thyroid 

Weight (mg) F2-F1 0.260 (0.255) 

0.031 

(0.242) 

0.242 

(0.259) 

0.368 

(0.251) 

0.422 

(0.258) 

Adult Left Thyroid Weight (mg) - 

Males F1-F0 0.211 (1.327) 

1.629 

(1.420) 

1.902 

(1.372) 

2.298 

(1.385) 

0.740 

(1.362) 

Adult Left Thyroid Weight (mg) - 

Females F1-F0 1.495 (0.938) 

1.039 

(0.921) 

-0.048 

(1.044) 

1.939 

(1.147) 

2.234 

(1.144) 

Sex Ratio

Ratio of Number of Males to Total 

Number of Offspring (%) F2-F1 -3.731 (3.806) 

-1.099 

(3.953) 

-2.630 

(3.597) 

3.758 

(3.904) 

2.113 

(3.106) 

a. random effects model with generation, concentration group and interaction of generation and concentration group treated as fixed 

effect, and laboratory, laboratory by generation and laboratory by generation by group as random effects, incorporating heterogeneous 

variances of group within laboratory and generation. 

b. The degrees of freedom for the fixed effects were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the 

total random variance across laboratories, Si

2

 and dfi are the reported variance and degrees of freedom within laboratory i, var(SR

2

) is 

the variance of SR

2

, and K is the number of studies (Hartung and Makambi, 2001) 

c. Differences of averages (least square means) between two generations and standard errors across laboratory for each concentration 

group were based on the above random effect models. 

d. Step-down trend tests were performed in the differences between the two generations. 

e. If generation or the interaction of generation and concentration group effect was significant, then F-tests were used to identify the 

concentration groups in which the two generations are significantly different at the 0.05 level. 

f. “↓” F2 or F1 generation less than F1or F0 generation. 

“↑” F2 or F1 generation greater than F1or F0 generation. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

115 

Table 17. (Continued). Differences between Generations across Laboratories. Chemical 

Vincolozolin, Avian Studya,b.

Parameter 

Difference 

Between 

Generations 

Difference of Averages Between Generations 

(Standard Error)c

Concentration 

Trend Testsd

Groups with 

Significant 

Difference 

between 

Generationse,f

Control 

(Group 1) 

64 ppm 

(Group 2)

160 ppm 

(Group 3)

400 ppm 

(Group 4)

1000 ppm 

(Group 5) 

Bio Chem

Egg Yolk Estradiol (pg/g) F1-F0 21.778 

(21.156) 

29.583 

(20.817) 

19.546 

(20.474) 

12.506 

(20.208) 

13.672 

(20.447) 

Egg Yolk Testosterone (pg/g) F1-F0 649.816 

(772.663) 

837.978 

(766.892) 

165.171 

(851.694) 

554.172 

(766.380) 

112.718 

(776.188) 

Plasma Thyroid Hormone T3 - 

Adult Males (ng/mL) F1-F0 -0.090 (0.148) 

0.076 

(0.163) 

-0.130 

(0.146) 

0.073 

(0.172) 

0.105 

(0.148) 

Plasma Thyroid Hormone T3 - 

Adult Females (ng/mL) F1-F0 -0.061 (0.100) 

-0.067 

(0.129) 

0.292 

(0.110) 

0.315 

(0.110) 

0.142 

(0.108) 

Increasing in 

difference (F1-F0) at 

the three highest 

concentrations 

Groups 3 () 

and 4() 

Plasma Thyroid Hormone T4 - 

Adult Males (ng/mL) F1-F0 0.211 (1.371) 

-1.771 

(1.473) 

-2.284 

(1.583) 

2.383 

(1.431) 

-0.692 

(1.335) 

Plasma Thyroid Hormone T4 - 

Adult Females (ng/mL) F1-F0 0.526 (0.820) 

-2.069 

(1.390) 

0.378 

(1.110) 

1.423 

(0.973) 

0.412 

(0.869) 

Plasma Sex Hormone Estradiol - 

Adult Males (pg/mL) F1-F0 1.031 (1.107) 

-0.132 

(0.808) 

-1.020 

(0.854) 

1.209 

(0.734) 

0.539 

(0.728) 

Plasma Sex Hormone Estradiol - 

Adult Females (pg/mL) F1-F0 -0.430 (4.627) 

-2.097 

(5.028) 

-0.941 

(4.579) 

0.819 

(5.507) 

2.326 

(4.704) 

Plasma Sex Hormone Testosterone 

- Adult Males (pg/mL) F1-F0 58.498 (737.559) 

-741.56 

(908.175) 

-535.74 

(901.060) 

-793.16 

(839.465) 

288.970 

(907.435) 

Plasma Sex Hormone Testosterone 

- Adult Females (pg/mL) F1-F0 4.146 (160.634) 

-2.638 

(190.094) 

369.037 

(182.549) 

80.781 

(170.390) 

-15.550 

(180.495) 

a. random effects model with generation, concentration group and interaction of generation and concentration group treated as fixed 

effect, and laboratory, laboratory by generation and laboratory by generation by group as random effects, incorporating heterogeneous 

variances of group within laboratory and generation. 

b. The degrees of freedom for the fixed effects were estimated as 2*((1/K)*∑(SR

2 + Si2

))2/(var(SR

2

)+(2/K2)*∑(Si

4

/dfi)), where SR

2

 is the 

total random variance across laboratories, Si

2

 and dfi are the reported variance and degrees of freedom within laboratory i, var(SR

2

) is 

the variance of SR

2

, and K is the number of studies (Hartung and Makambi, 2001) 

c. Differences of averages (least square means) between two generations and standard errors across laboratory for each concentration 

group were based on the above random effect models. 

d. Step-down trend tests were performed in the differences between the two generations. 

e. If generation or the interaction of generation and concentration group effect was significant, then F-tests were used to identify the 

concentration groups in which the two generations are significantly different at the 0.05 level. 

f. “↓” F2 or F1 generation less than F1or F0 generation. 

“↑” F2 or F1 generation greater than F1or F0 generation. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

116 

6. Comparison of Inter-laboratory CVs and Within-study Sensitivity across Endpoints 

6.1 Comparison of Inter-laboratory CVs across Endpoints 

The avian assay parameters were divided into 9 categories: 

 Body Weight 

 Feed Consumption 

 Reproduction 

 Egg Shell and Weight 

 Thyroid Weight 

 Bio Chem 

 First Foam First Egg Cloacal Size 

 Mating Behavior 

 Sex Ratio 

 For each parameter, the CV across labs and the associated p-value for significance of the 

laboratory random variation (i.e. differences among laboratories) for each generation were summarized 

and are shown in Table 18. The ranges of CVs were compared across categories. Within each category, 

the numbers of endpoints with larger CV for a later generation than a previous generation are given, based 

on the common endpoints in the generations. These are shown in Table 19. The CVs are displayed in 

Figure 1 through Figure 10, in which CVs for one generation are plotted against those for the subsequent 

generation. The reference lines correspond to equality of CVs for the two generations. Parameters lying 

above the reference line have greater percent CV for the generation in y-axis than the %CV for the 

generation in the x-axis. For each endpoint (as represented by a point on the graph), the closer the point is 

to the line, the more agreement there is in the CV values between the two generations. 

The following are some findings: 

 There are no endpoints that were determined in all three generations. Within the 

same category, two generations may measure different endpoints. For some 

categories, some generations could not be compared. Generation F0 can be 

compared with generation F1 for 6 out of 9 categories, generation F1 can be 

compared with generation F2 for 4 out of 9 categories, and generation F0 cannot be 

compared with generation F2 for any category. 

 The median of F1 CVs exceeds that of the F0 CVs for 27 of 57 (47%) comparsions. 

This is nearly 50% so there is no systematic generation difference with respect to 

CVs. Some of the highest CVs are in the F1 generation, particularly for mating 

behavior. 

 The median of F2 CVs exceeds that of the F1 CVs in 15 of 24 (62.5%) comparisons. 

This possibly suggests some degradation in sensitivity in F2 compared to F1. 

 The smaller CVs for body weight in the F2 generation may be due to the fact that 

adult body weights were not measured for the F2 generation. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

117 

6.2 Comparison of Within-study Power Sensitivity across Endpoints 

Power analyses were carried out to determine deviations from the control group means that could 

be detected with power 80% at the 5% (two-sided) significance level. The power analyses were based on 

the pooled average within-laboratory variability for the control group parameters across the withinlaboratory studies. The sample sizes assumed are the nominal sample sizes for the control group. Table 

20 displays the control group means, the sample sizes, the percent distances from the control group means 

that can be detected with power 80% for each generation (F0, F1, or F2), and the ratios of the F1 percent 

deviation to the F0 percent deviation and the F2 percent deviation to the F1 percent deviation for the 

corresponding parameters. There is no ratio of generation F2 to generation F0 since there is no endpoint 

that was measured in both F0 and F2 generations. 

The ranges of percent deviations to attain high power were compared across categories. Within 

each category, the numbers of endpoints with larger percent deviation for a later generation than a 

previous generation are given, based on the common endpoints in the generations. These are shown in 

Table 21. 

F1 was compared to F0 for six of nine categories. The F1 percent deviation exceeded the F0 

percent deviation for 34 of 57 comparisons (60%). This possibly suggests some degradation in sensitivity 

in F1 compared to F0. The F2 percent deviation exceeded the F1 percent deviation for 14 of 24 

comparisons (58%). This again possibly suggests some degradation in sensitivity in F2 compared to F1. 

Figure 11 through Figure 20 display the F0 vs. F1 and F1 vs. F2 percent deviations from the 

control group means for the corresponding parameters for detection with 80% power with 5% (two-sided) 

significance level. The reference lines correspond to equality of the percent deviations. Parameters lying 

above the reference line have greater percent deviation for the generation in y-axis than percent deviation 

for the generation in the x-axis. 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

118 

Table 18. CV (%) and Significance Levels across Laboratories 

Parameter 

CV (%) 

P-value 

Random Laboratories 

F0 F1 F2 F0 F1 F2 

Body Weight 

Adult Body Weight (g) Day 0 - Males 28.731 27.911 . <.0001 <.0001 . 

Adult Body Weight (g) Day 56 - Males 29.578 30.273 . <.0001 <.0001 . 

Adult Body Weight (g) Day 0 - Females 27.335 31.107 . <.0001 <.0001 . 

Adult Body Weight (g) Day 56 - Females 27.559 28.782 . <.0001 <.0001 . 

Mean Offspring Body Weight by Pen (g) Day 0 . 17.213 16.846 . <.0001 <.0001 

Mean Offspring Body Weight by Pen (g) Day 14 . 26.774 24.773 . <.0001 <.0001 

Feed Consumption 

Adult Feed Consumption (g/bird/day) - Week 1 25.855 22.290 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 2 18.472 26.236 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 3 21.011 21.365 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 4 18.640 18.663 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 5 19.282 14.896 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 6 23.831 18.508 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 7 17.378 18.443 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 8 16.933 20.224 . <.0001 <.0001 . 

Adult Feed Consumption (g/bird/day) - Week 9 . 2.510 . . 1.0000 . 

Adult Feed Consumption (g/bird/day) - Week 10 . . . . . . 

Adult Feed Consumption (g/bird/day) - Week 11 . . . . . . 

Reproduction 

Eggs Laid 7.089 7.109 . 0.0301 0.0854 . 

Eggs Crack 103.138 59.020 . 0.0204 0.3369 . 

Eggs Abnormal 98.889 112.195 . 0.0457 0.0325 . 

Eggs Set From Weeks 7 and 8 7.319 9.070 . 0.1170 0.0318 . 

Eggs Set From Weeks 6 7 and 8 8.241 54.635 . 0.1051 <.0001 . 

Eggs Fertile From Weeks 7 and 8 9.665 9.448 . 0.0560 0.3011 . 

Eggs Fertile From Weeks 6 7 and 8 11.157 55.649 . 0.0280 <.0001 . 

Eggs Viable From Weeks 7 and 8 12.603 32.387 . 0.0067 <.0001 . 

Eggs Viable From Weeks 6 7 and 8 15.977 54.416 . 0.0004 <.0001 . 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 12.020 31.518 . 0.0129 <.0001 . 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 15.526 6.381 . 0.0008 0.8958 . 

Eggs Hatched 17.983 12.270 . 0.0008 0.2336 . 

Number of 14-Day-Old Surviving Chicks 12.594 11.370 . 0.0842 0.3893 . 

Eggs Laid Per Hen Per Day 9.212 7.000 . 0.0026 0.0916 . 

Cracked Eggs as A Percentage of Eggs Laid 103.129 64.778 . 0.0157 0.3989 . 

Abnormal Eggs as A Percentage of Eggs Laid 103.321 120.762 . 0.0623 0.0798 . 

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 2.025 2.560 . 0.7854 0.8054 . 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

119 

Parameter 

CV (%) 

P-value 

Random Laboratories 

F0 F1 F2 F0 F1 F2 

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 2.765 1.962 . 0.2591 1.0000 . 

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 4.756 2.965 . 0.1002 1.0000 . 

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 7.136 2.791 . 0.0099 1.0000 . 

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 7 and 8 0.653 0.920 . 0.5758 0.5293 . 

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as A 

Percentage of Viable Eggs From Weeks 6 7 and 8 0.541 0.594 . 0.6127 0.7466 . 

Eggs Hatched as A Percentage of Number of Viable Eggs from Weeks 7 and 8 

after Two Weeks of Incubation 6.318 5.124 . 0.1036 0.0670 . 

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs Hatched 11.547 1.673 . <.0001 0.8747 . 

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 and 8 10.735 5.421 . 0.0070 1.0000 . 

Eggs Hatched as A Percentage of Number of Fertile Eggs from Weeks 7 and 8 8.615 3.800 . 0.0251 1.0000 . 

Number of 14-Day-Old Surviving Chicks as A Percentage of Number of Eggs 

Set from Weeks 7 and 8 7.806 5.733 . 0.2429 1.0000 . 

Eggs Hatched Per Hen Per Day 18.017 12.266 . 0.0008 0.2353 . 

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 12.478 11.398 . 0.0926 0.3874 . 

Egg Shell and Weight

Pen Mean Egg Shell Thickness (mm) 7.610 6.688 . <.0001 <.0001 . 

Pen Mean Egg Content Weight - Albumin (g) 19.591 20.453 . <.0001 <.0001 . 

Pen Mean Egg Content Weight - Yolk (g) 11.142 8.447 . <.0001 <.0001 . 

Pen Mean Egg Content Weight - Combined (g) 10.459 13.906 . <.0001 <.0001 . 

First Foam First Egg Cloacal Size 

Age in Days at First Foam - Males . 8.894 . . 0.0004 . 

Age in Days at First Egg - Females . 5.801 . . 0.0357 . 

Cloacal Area (cm2) at 4 Weeks - Males . 14.097 . . 0.1126 . 

Cloacal Area (cm2) at 5 Weeks - Males . 31.742 . . 0.0107 . 

Cloacal Area (cm2) at 6 Weeks - Males . 21.997 . . <.0001 . 

Cloacal Area (cm2) at 7 Weeks - Males . . . . . . 

Cloacal Area (cm2) at 8 Weeks - Males . . . . . . 

Cloacal Area (cm2) at 9 Weeks - Males . . . . . . 

Cloacal Area (cm2) at 4 Weeks - Females . 11.533 . . 0.6327 . 

Cloacal Area (cm2) at 5 Weeks - Females . 64.012 . . <.0001 . 

Cloacal Area (cm2) at 6 Weeks - Females . 81.865 . . <.0001 . 

Cloacal Area (cm2) at 7 Weeks - Females . . . . . . 

Cloacal Area (cm2) at 8 Weeks - Females . . . . . . 

Cloacal Area (cm2) at 9 Weeks - Females . . . . . . 

Mating Behavior

Length of Time to First Neck Grab (sec) - Day 1 . 59.392 83.568 . 0.6561 1.0000 

Length of Time to First Neck Grab (sec) - Day 2 . 29.454 92.882 . 0.8696 1.0000 

Length of Time to First Neck Grab (sec) - Day 3 . 12.409 92.736 . 1.0000 1.0000 
DRAFT 

Inter-Laboratory Statistical Analysis Report for Avian 2-Tier Test 

120 

Parameter 

CV (%) 

P-value 

Random Laboratories 

F0 F1 F2 F0 F1 F2 

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 . 79.706 145.584 . 0.6773 1.0000 

Length of Time to First Mount (sec) - Day 1 . 56.537 73.901 . 1.0000 1.0000 

Length of Time to First Mount (sec) - Day 2 . 44.005 75.943 . 1.0000 1.0000 

Length of Time to First Mount (sec) - Day 3 . 24.847 90.089 . 1.0000 1.0000 

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 . 73.821 54.930 . 1.0000 1.0000 

Number of Mount Attempts (count) - Day 1 . 21.778 70.141 . 0.7640 0.0590 

Number of Mount Attempts (count) - Day 2 . 58.076 63.418 . 0.0514 0.2018 

Number of Mount Attempts (count) - Day 3 . 48.592 39.164 . 0.4330 0.5167 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 . 272.469 196.641 . 0.0103 1.0000 

Number of Cloacal Contacts (count) - Day 1 . 32.484 31.752 . 0.7605 1.0000 

Number of Cloacal Contacts (count) - Day 2 . 42.822 53.720 . 0.6816 0.4102 

Number of Cloacal Contacts (count) - Day 3 . 57.318 46.839 . 0.0812 0.2296 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 . 112.664 63.888 . 0.2418 0.8564 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 . 76.604 117.058 . <.0001 0.0544 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 . 67.035 113.624 . 0.0008 <.0001 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 . 100.826 82.659 . <.0001 0.0045 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 to Day 3 . 157.952 208.837 . 0.0053 1.0000 

Thyroid Weight

14-Day Old Left Thyroid Weight (mg) - Males . . 50.623 . . <.0001 

14-Day Old Left Thyroid Weight (mg) - Females . . 14.520 . . 0.8981 

Adult Left Thyroid Weight (mg) - Males 49.818 30.290 . <.0001 0.0105 . 

Adult Left Thyroid Weight (mg) - Females 30.659 42.334 . <.0001 <.0001 . 

15 Day Embryo (ED15) Thyroid Weight (mg) . 40.269 53.455 . <.0001 <.0001 

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) . 9.099 13.060 . 1.0000 0.3228 

Bio Chem 

Egg Yolk Estradiol (pg/g) 57.540 34.223 . <.0001 0.1630 . 

Egg Yolk Testosterone (pg/g) 20.125 56.217 . 1.0000 0.0005 . 

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 40.598 23.023 . <.0001 0.1021 . 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 22.221 12.462 . 0.0077 0.5207 . 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 16.968 61.524 . 1.0000 0.0144 . 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 50.953 24.484 . 0.2702 0.9711 . 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 20.275 22.599 . 0.5539 1.0000 . 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 38.479 18.807 . <.0001 0.8200 . 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 55.003 67.775 . 0.0345 <.0001 . 

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 30.048 23.132 . 1.0000 1.0000 . 

Plasma Thyroid Hormone T3 - 14-Day Old Males (ng/mL) . . 51.471 . . <.0001 

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL) . . 47.806 . . 0.2479 
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Parameter 

CV (%) 

P-value 

Random Laboratories 

F0 F1 F2 F0 F1 F2 

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL) . . 63.781 . . 0.0075 

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL) . . 33.754 . . 1.0000 

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) . . 40.164 . . 0.1843 

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) . . 18.056 . . 0.8433 

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL) . . 79.734 . . <.0001 

Plasma Sex Hormone Testosterone - 14-Day Old Females (pg/mL) . . 81.519 . . <.0001 

Table 19. Ranges of CV% across Laboratories. By Category. 

Category 

F0 Generation F1 Generation F2 Generation 

# F1 > F0 # F2 > F1 

CV Range Median CV Range Median CV Range Median 

Body Weight 27.3 – 29.6 28.1 17.2 – 31.1 28.3 16.8 – 24.8 16.8 3/4 0/2 

Feed 

Consumption 16.9 – 25.9 19.0 2.5 – 26.2 18.7 NA NA 5/8 NA 

Reproduction 0.54 –103.3 10.7 0.59 - 120.8 9.1 NA NA 12/29 NA 

Egg Shell 

and Weight 7.6 – 19.6 10.8 6.7 – 20.5 11.2 NA NA 2/4 NA 

First Foam 

First Egg 

Cloacal Size 

NA NA 5.8 – 81.9 18.0 NA NA NA NA 

Mating 

Behavior NA NA 12.4 – 272.5 57.7 31.8 – 208.8 79.3 NA 13/20 

Thyroid 

Weight 30.7 – 49.8 40.2 30.3 – 42.3 40.3 14.5 – 53.5 50.6 1/2 1/1 

Sex Ratio NA NA 9.1 – 9.1 9.1 13.1 – 13.1 13.1 NA 1/1 

Bio Chem 17.0 – 57.5 34.2 12.5 – 67.8 23.8 18.1 – 81.5 49.6 4/10 NA 

Total 0.54 – 103.3 17.4 0.59 – 272.3 23.1 13.1 – 208.8 63.6 27/57 15/24 
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Table 20. Percent Deviations from Control Group Means for Detections with 80% Power and the Ratios Between G

Parameter 

Control Mean Sample Size (N) Percent Distances from Contr

F0 F1 F2 F0 F1 F2 F0 F1 F2 

Body Weight 

Adult Body Weight (g) Day 0 - Males 168.849 138.313 . 18 18 . 8.085 7.950 . 

Adult Body Weight (g) Day 56 - Males 184.637 182.906 . 18 18 . 7.282 8.926 . 

Adult Body Weight (g) Day 0 - Females 207.898 149.912 . 18 18 . 10.463 10.835 . 

Adult Body Weight (g) Day 56 - Females 227.716 228.910 . 18 18 . 9.289 10.433 . 

Mean Offspring Body Weight by Pen (g) Day 0 . 8.365 8.060 . 18 18 . 8.346 8.450 

Mean Offspring Body Weight by Pen (g) Day 14 . 70.035 67.491 . 18 18 . 6.870 8.043 

Feed Consumption

Adult Feed Consumption (g/bird/day) - Week 1 26.402 27.262 . 18 18 . 10.477 9.242 . Adult Feed Consumption (g/bird/day) - Week 2 26.199 29.728 . 18 18 . 14.503 9.101 . Adult Feed Consumption (g/bird/day) - Week 3 25.203 28.659 . 18 18 . 12.684 12.560 . Adult Feed Consumption (g/bird/day) - Week 4 26.791 26.854 . 18 18 . 13.842 11.983 . Adult Feed Consumption (g/bird/day) - Week 5 29.183 26.431 . 18 18 . 12.045 13.013 . Adult Feed Consumption (g/bird/day) - Week 6 28.979 27.453 . 18 18 . 11.120 12.574 . Adult Feed Consumption (g/bird/day) - Week 7 28.524 28.391 . 18 18 . 9.941 9.781 . Adult Feed Consumption (g/bird/day) - Week 8 26.849 27.657 . 18 18 . 8.446 14.263 . Adult Feed Consumption (g/bird/day) - Week 9 . 31.400 . . 18 . . 12.895 . 

Reproduction

Eggs Laid 42.897 43.527 . 18 18 . 16.726 14.458 . 

Eggs Crack 0.907 1.014 . 18 18 . 246.207 153.820 . 

Eggs Abnormal 1.269 1.304 . 18 18 . 256.171 166.199 . 

Eggs Set From Weeks 7 and 8 11.678 11.447 . 18 18 . 15.390 19.527 . 

Eggs Set From Weeks 6 7 and 8 17.440 24.007 . 18 18 . 15.349 16.836 . 

Eggs Fertile From Weeks 7 and 8 11.249 10.954 . 18 18 . 17.299 25.433 . 

Eggs Fertile From Weeks 6 7 and 8 16.867 23.020 . 18 18 . 17.079 20.145 . 
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Parameter 

Control Mean Sample Size (N) Percent Distances from Contr

F0 F1 F2 F0 F1 F2 F0 F1 F2 

Eggs Viable From Weeks 7 and 8 10.915 12.528 . 18 18 . 17.654 25.215 . 

Eggs Viable From Weeks 6 7 and 8 16.202 22.290 . 18 18 . 17.678 21.977 . 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation 10.841 12.377 . 18 18 . 17.724 26.276 . 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation 16.106 15.873 . 18 18 . 17.842 25.215 . 

Eggs Hatched 9.907 10.100 . 18 18 . 22.325 31.346 . 

Number of 14-Day-Old Surviving Chicks 8.790 9.676 . 18 18 . 30.497 32.359 . 

Eggs Laid Per Hen Per Day 0.902 0.889 . 18 18 . 12.694 14.443 . 

Cracked Eggs as A Percentage of Eggs Laid 0.022 0.025 . 18 18 . 240.434 166.368 . 

Abnormal Eggs as A Percentage of Eggs Laid 0.032 0.033 . 18 18 . 265.997 192.231 . 

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.969 0.970 . 18 18 . 7.718 13.710 . 

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.970 0.959 . 18 18 . 6.567 8.953 . 

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8 0.944 0.945 . 18 18 . 9.802 13.835 . 

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8 0.935 0.927 . 18 18 . 9.965 12.043 . 

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation as 

A Percentage of Viable Eggs From Weeks 7 and 8 0.995 0.995 . 18 18 . 2.338 4.228 . 

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation as 

A Percentage of Viable Eggs From Weeks 6 7 and 8 0.996 0.997 . 18 18 . 1.834 3.962 . 

Eggs Hatched as A Percentage of Number of Viable Eggs from 

Weeks 7 and 8 after Two Weeks of Incubation 0.910 0.944 . 18 18 . 12.226 13.150 . 

Number of 14-Day-Old Surviving Chicks as A Percentage of Eggs 

Hatched 0.899 0.964 . 18 18 . 16.909 6.799 . 

Eggs Hatched as A Percentage of Number of Eggs Set from Weeks 7 

and 8 0.852 0.866 . 18 18 . 16.434 22.202 . 

Eggs Hatched as A Percentage of Number of Fertile Eggs from 

Weeks 7 and 8 0.881 0.913 . 18 18 . 14.343 16.377 . 

Number of 14-Day-Old Surviving Chicks as A Percentage of 

Number of Eggs Set from Weeks 7 and 8 0.768 0.834 . 18 18 . 25.006 23.586 . 

Eggs Hatched Per Hen Per Day 0.708 0.722 . 18 18 . 22.371 31.395 . 

Number of 14-Day-Old Surviving Chicks Per Hen Per Day 0.627 0.691 . 18 18 . 30.555 32.405 . 
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Parameter 

Control Mean Sample Size (N) Percent Distances from Contr

F0 F1 F2 F0 F1 F2 F0 F1 F2 

Egg Shell and Weight

Pen Mean Egg Shell Thickness (mm) 0.215 0.213 . 18 18 . 6.470 6.166 . 

Pen Mean Egg Content Weight - Albumin (g) 4.743 5.257 . 18 18 . 14.133 11.911 . 

Pen Mean Egg Content Weight - Yolk (g) 3.640 3.626 . 18 18 . 14.692 10.611 . 

Pen Mean Egg Content Weight - Combined (g) 8.394 8.881 . 18 18 . 12.688 10.025 . 

First Foam First Egg Cloacal Size

Age in Days at First Foam - Males . 37.227 . . 18 . . 22.312 . 

Age in Days at First Egg - Females . 44.964 . . 18 . . 11.818 . 

Cloacal Area (cm2) at 4 Weeks - Males . 0.255 . . 18 . . 31.289 . Cloacal Area (cm2) at 5 Weeks - Males . 0.384 . . 18 . . 51.638 . Cloacal Area (cm2) at 6 Weeks - Males . 0.546 . . 18 . . 24.797 . 

Cloacal Area (cm2) at 4 Weeks - Females . 0.249 . . 18 . . 39.008 . Cloacal Area (cm2) at 5 Weeks - Females . 0.336 . . 18 . . 70.697 . Cloacal Area (cm2) at 6 Weeks - Females . 0.515 . . 18 . . 57.919 . 

Mating Behavior

Length of Time to First Neck Grab (sec) - Day 1 . 10.208 50.218 . 7 7 . 332.825 360.773 Length of Time to First Neck Grab (sec) - Day 2 . 6.393 37.017 . 7 7 . 137.522 427.554 Length of Time to First Neck Grab (sec) - Day 3 . 3.118 45.719 . 7 7 . 92.526 400.35

Change in Length of Time to First Neck Grab (sec) from Day 1 to 

Day 3 . -6.676 -2.243 . 7 7 . 484.413 1072.17

Length of Time to First Mount (sec) - Day 1 . 44.713 55.717 . 7 7 . 333.232 319.03Length of Time to First Mount (sec) - Day 2 . 14.351 62.243 . 7 7 . 732.628 338.194 Length of Time to First Mount (sec) - Day 3 . 5.256 46.868 . 7 7 . 546.313 388.940 

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 . -29.801 -6.785 . 7 7 . 493.118 367.122 

Number of Mount Attempts (count) - Day 1 . 6.055 5.862 . 7 7 . 80.840 120.344 Number of Mount Attempts (count) - Day 2 . 7.405 7.517 . 7 7 . 139.991 138.865 Number of Mount Attempts (count) - Day 3 . 7.425 6.673 . 7 7 . 123.790 114.13
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Parameter 

Control Mean Sample Size (N) Percent Distances from Contr

F0 F1 F2 F0 F1 F2 F0 F1 F2 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 . 1.896 0.972 . 7 7 . 433.539 892.147 

Number of Cloacal Contacts (count) - Day 1 . 2.339 2.542 . 7 7 . 139.226 137.262 Number of Cloacal Contacts (count) - Day 2 . 3.075 3.482 . 7 7 . 166.594 146.037 Number of Cloacal Contacts (count) - Day 3 . 4.203 3.200 . 7 7 . 102.131 105.26

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 . 1.811 1.000 . 7 7 . 259.833 270.003 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 . 4.859 3.500 . 7 7 . 84.508 199.09Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 . 2.863 3.288 . 7 7 . 128.466 96.549 Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 . 3.425 2.931 . 7 7 . 95.846 105.224 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from 

Day 1 to Day 3 . -1.460 0.241 . 7 7 . 355.194 3361.24

Thyroid Weight

14-Day Old Left Thyroid Weight (mg) - Males . . 2.954 . . 18 . . 39.774 

14-Day Old Left Thyroid Weight (mg) - Females . . 2.245 . . 18 . . 57.242 

Adult Left Thyroid Weight (mg) - Males 7.348 7.345 . 18 18 . 53.943 45.910 . 

Adult Left Thyroid Weight (mg) - Females 9.096 11.506 . 18 18 . 40.219 48.355 . 

15 Day Embryo (ED15) Thyroid Weight (mg) . 1.795 2.051 . 18 18 . 38.273 45.884 

Sex Ratio 

Ratio of Number of Males to Total Number of Offspring (%) . 53.085 48.939 . 18 18 . 40.136 34.590 

Bio Chem 

Egg Yolk Estradiol (pg/g) 44.421 67.845 . 5 5 . 49.027 83.636 . 

Egg Yolk Testosterone (pg/g) 2050.636 3041.891 . 5 5 . 110.604 69.641 . 

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) 0.871 0.748 . 5 5 . 63.551 54.413 . 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) 0.860 0.815 . 5 5 . 43.085 44.612 . 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) 3.164 3.722 . 5 5 . 120.061 126.798 . 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) 1.763 2.195 . 5 5 . 181.151 134.390 . 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) 4.611 5.569 . 5 5 . 96.557 258.880 . 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) 18.452 16.668 . 5 5 . 64.326 90.699 . 
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Parameter 

Control Mean Sample Size (N) Percent Distances from Contr

F0 F1 F2 F0 F1 F2 F0 F1 F2 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) 1694.232 1759.598 . 5 5 . 98.022 93.081 . 

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) 337.621 385.548 . 5 5 . 273.307 168.710 . 

Plasma Thyroid Hormone T3 - 14-Day Old Males (ng/mL) . . 0.943 . . 5 . . 43.803 

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL) . . 1.320 . . 5 . . 123.170 

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL) . . 2.597 . . 5 . . 157.50

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL) . . 2.864 . . 5 . . 156.170 

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) . . 4.016 . . 5 . . 137.446 

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) . . 11.992 . . 5 . . 75.412 

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL) . . 23.526 . . 5 . . 132.89

Plasma Sex Hormone Testosterone - 14-Day Old Females (pg/mL) . . 18.332 . . 5 . . 198.495 
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Table 21. Ranges of Percent Deviations from Control Group Means for Detections with 80% Power and the Ratios 

By Category. 

Category 

Percent Distances from Control Ratio between Generation in Percent Distance

Control 

F0 Generation F1 Generation F2 Generation F1/F0 F2/F1 

Range Median Range Median Range Median Range Median Range M

Body Weight 

7.282-

10.463 

8.687 

6.870-

10.835 

8.636 

8.043-

8.450 

8.246 

0.983-

1.226 

1.079 

1.012-

1.171 

1

Feed Consumption 

8.446-

14.503 

11.583 

9.101-

14.263 

12.560 NA NA 

0.628-

1.689 

0.987 NA 

Reproduction 

1.834-

265.997 

17.079 

3.962-

192.231 

21.977 NA NA 

0.402-

2.161 

1.208 NA 

Egg Shell and Weight 

6.470-

14.692 

13.411 

6.166-

11.911 

10.318 NA NA 

0.722-

0.953 

0.816 NA 

First Foam First Egg 

Cloacal Size NA NA 

11.818-

70.697 

35.149 NA NA NA NA NA 

Mating Behavior NA NA 

80.840-

732.628 

153.293 

96.549-

3361.243 

294.521 NA NA 

0.462-

9.463 

1

Thyroid Weight 

40.219-

53.943 

47.081 

38.273-

48.355 

45.910 

39.774-

57.242 

45.884 

0.851-

1.202 

1.027 

1.199-

1.199 

1

Sex Ratio NA NA 

40.136-

40.136 

40.136 

34.590-

34.590 

34.590 NA NA 

0.862-

0.862 

0

Bio Chem 

43.085-

273.307 

97.290 

44.612-

258.880 

91.890 

43.803-

198.495 

135.169 

0.617-

2.681 

0.993 NA 

Total 

1.834-

273.307 

16.726 

3.962-

732.628 

31.371 

8.043-

3361.243 

138.156 

0.402-

2.681 

1.076 

0.462-

9.463 

1
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Figure 1. F0 CV% vs. F1 CV% for Corresponding Parameters. Body Weight.

% CV (F1)

202224262830323436

% CV (F0)

20 22 24 26 28 30 32 3

1-Body Weight Results

Equal Line
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Figure 2. F1 CV% vs. F2 CV% for Corresponding Parameters. Body Weight. 

% CV (F2)

151821242730

% CV (F1)

15 18 21 24 27 

1-Body Weight Results

Equal Line
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Figure 3. F0 CV% vs. F1 CV% for Corresponding Parameters. Feed Consumption.

% CV (F1)

1012141618202224262830

% CV (F0)

10 12 14 16 18 20 22 24 26 

2-Feed Consumption Results

Equal Line
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Figure 4. F0 CV% vs. F1 CV% for Corresponding Parameters. Reproduction.

% CV (F1)

0

20406080

100120

% CV (F0)

0 20 40 60 80 100 

3-Reproduction Results

Equal Line
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Figure 5. F0 CV% vs. F1 CV% for Corresponding Parameters. Egg Shell and Weight.

% CV (F1)

56789

10111213141516171819202122232425

% CV (F0)

5 10 15 20 

4-Egg Shell and Weight Results

Equal Line
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Figure 6. F1 CV% vs. F2 CV% for Corresponding Parameters. Mating Behavior.

% CV (F2)

0

306090

120150180210240270300

% CV (F1)

0 30 60 90 120 150 180 210 240 

6-Mating Behavior Results

Equal Line
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Figure 7. F0 CV% vs. F1 CV% for Corresponding Parameters. Thyroid Weight.

% CV (F1)

202530354045505560

% CV (F0)

20 25 30 35 40 45 50 

7-Thyroid Weight Results

Equal Line
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Figure 8. F1 CV% vs. F2 CV% for Corresponding Parameters. Thyroid Weight.

% CV (F2)

30354045505560

% CV (F1)

30 35 40 45 50 55 

7-Thyroid Weight Results

Equal Line
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Figure 9. F1 CV% vs. F2 CV% for Corresponding Parameters. Sex Ratio.

% CV (F2)

56789

101112131415

% CV (F1)

5 6 7 8 9 10 11 12 13 

8-Sex Ratio Results

Equal Line
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Figure 10. F0 CV% vs. F1 CV% for Corresponding Parameters. Bio Chem. 

% CV (F1)

10203040506070

% CV (F0)

10 20 30 40 50 60 

9-Bio Chem Results

Equal Line
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Figure 11. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Body Weight.

Percent Distance From Control Mean (F1)

789

1011

Percent Distance From Control Mean (F0)

7 8 9 10 

1-Body Weight Results

Equal Line
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Figure 12. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Body Weight.

Percent Distance From Control Mean (F2)

6789

10

Percent Distance From Control Mean (F1)

6 7 8 9 

1-Body Weight Results

Equal Line
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Figure 13. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Feed Consumption.

Percent Distance From Control Mean (F1)

89

101112131415

Percent Distance From Control Mean (F0)

8 9 10 11 12 13 14 

2-Feed Consumption Results

Equal Line
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Figure 14. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Reproduction.

Percent Distance From Control Mean (F1)

0

50

100150200250300

Percent Distance From Control Mean (F0)

0 50 100 150 200 250 

3-Reproduction Results

Equal Line
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Figure 15. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Egg Shell and Wight.

Percent Distance From Control Mean (F1)

56789

101112131415

Percent Distance From Control Mean (F0)

5 6 7 8 9 10 11 12 13 

4-Egg Shell and Weight Results

Equal Line
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Figure 16. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Mating Behavior.

Percent Distance From Control Mean (F2)

0

500

1000150020002500300035004000

Percent Distance From Control Mean (F1)

0 100 200 300 400 500 600 700 800 

6-Mating Behavior Results

Equal Line
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Figure 17. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Thyroid Weight.

Percent Distance From Control Mean (F1)

30354045505560

Percent Distance From Control Mean (F0)

30 35 40 45 50 55 

7-Thyroid Weight Results

Equal Line
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Figure 18. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Thyroid Weight.

Percent Distance From Control Mean (F2)

30354045505560

Percent Distance From Control Mean (F1)

30 35 40 45 50 55 

7-Thyroid Weight Results

Equal Line
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Figure 19. F1 vs. F2 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Sex Ratio. 

Percent Distance From Control Mean (F2)

30354045505560

Percent Distance From Control Mean (F1)

30 35 40 45 50 55 

8-Sex Ratio Results

Equal Line
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Figure 20. F0 vs. F1 Percent Deviations from the Control Group Means for the Corresponding Parameters to Dete

80%. Bio Chem.

Percent Distance From Control Mean (F1)

0

50

100150200250300

Percent Distance From Control Mean (F0)

0 50 100 150 200 250 

9-Bio Chem Results

Equal Line
 

Appendix A 

Figures for Avian Control Data 

Means ± 2 Standard Errors 
DRAFT 

Avian Control Data 

A-1 

Means ± 2 Standard Errors for

Adult Body Weight (g) Day 0 - Males

8090

100110120130140150160170180190200210220230

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-2 

Means ± 2 Standard Errors for

Adult Body Weight (g) Day 56 - Males

110120130140150160170180190200210220230240250

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-3 

Means ± 2 Standard Errors for

Adult Body Weight (g) Day 0 - Females

8090

100110120130140150160170180190200210220230240250260270280

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-4 

Means ± 2 Standard Errors for

Adult Body Weight (g) Day 56 - Females

140150160170180190200210220230240250260270280290300310

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-5 

Means ± 2 Standard Errors for

Mean Offspring Body Weight by Pen (g) Day 0

6789

1011

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-6 

Means ± 2 Standard Errors for

Mean Offspring Body Weight by Pen (g) Day 14

405060708090

100

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-7 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 1

17181920212223242526272829303132333435

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-8 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 2

192021222324252627282930313233343536373839

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-9 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 3

18192021222324252627282930313233343536

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-10 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 4

20212223242526272829303132333435

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-11 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 5

21222324252627282930313233343536

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-12 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 6

212223242526272829303132333435363738

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-13 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 7

212223242526272829303132333435

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-14 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 8

2021222324252627282930313233343536

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-15 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 9

293031323334353637

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-16 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 10

2930313233

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-17 

Means ± 2 Standard Errors for

Adult Feed Consumption (g/bird/day) - Week 11

2324252627282930

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-18 

Means ± 2 Standard Errors for

Eggs Laid

35363738394041424344454647484950

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-19 

Means ± 2 Standard Errors for

Eggs Crack

-1

01234

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-20 

Means ± 2 Standard Errors for

Eggs Abnormal

-1

0123456

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-21 

Means ± 2 Standard Errors for

Eggs Set From Weeks 7 and 8

9

1011121314

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-22 

Means ± 2 Standard Errors for

Eggs Set From Weeks 6 7 and 8

0

1020304050

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-23 

Means ± 2 Standard Errors for

Eggs Fertile From Weeks 7 and 8

89

1011121314

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-24 

Means ± 2 Standard Errors for

Eggs Fertile From Weeks 6 7 and 8

0

1020304050

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-25 

Means ± 2 Standard Errors for

Eggs Viable From Weeks 7 and 8

789

10111213141516171819

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-26 

Means ± 2 Standard Errors for

Eggs Viable From Weeks 6 7 and 8

0

10203040

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-27 

Means ± 2 Standard Errors for

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation

789

10111213141516171819

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-28 

Means ± 2 Standard Errors for

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation

111213141516171819

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-29 

Means ± 2 Standard Errors for

Eggs Hatched

6789

10111213

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-30 

Means ± 2 Standard Errors for

Number of 14-Day-Old Surviving Chicks

6789

10111213

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-31 

Means ± 2 Standard Errors for

Eggs Laid Per Hen Per Day

0.710.720.730.740.750.760.770.780.790.800.810.820.830.840.850.860.870.880.890.900.910.920.930.940.950.960.970.980.991.001.011.021.031.041.05

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-32 

Means ± 2 Standard Errors for

Cracked Eggs as A Percentage of Eggs Laid

-0.01

0.000.010.020.030.040.050.060.070.08

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-33 

Means ± 2 Standard Errors for

Abnormal Eggs as A Percentage of Eggs Laid

-0.02-0.01

0.000.010.020.030.040.050.060.070.080.090.100.110.120.130.140.15

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-34 

Means ± 2 Standard Errors for

Fertile Eggs as A Percentage of Eggs Set From Weeks 7 and 8

0.840.850.860.870.880.890.900.910.920.930.940.950.960.970.980.991.001.011.021.031.04

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-35 

Means ± 2 Standard Errors for

Fertile Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8

0.870.880.890.900.910.920.930.940.950.960.970.980.991.001.01

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-36 

Means ± 2 Standard Errors for

Viable Eggs as A Percentage of Eggs Set From Weeks 7 and 8

0.820.830.840.850.860.870.880.890.900.910.920.930.940.950.960.970.980.991.001.01

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-37 

Means ± 2 Standard Errors for

Viable Eggs as A Percentage of Eggs Set From Weeks 6 7 and 8

0.770.780.790.800.810.820.830.840.850.860.870.880.890.900.910.920.930.940.950.960.970.980.991.001.011.02

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-38 

Means ± 2 Standard Errors for

Viable Eggs from Weeks 7 and 8 after Two Weeks of Incubation

as A Percentage of Viable Eggs From Weeks 7 and 8

0.940.950.960.970.980.991.001.011.02

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-39 

Means ± 2 Standard Errors for

Viable Eggs from Weeks 6 7 and 8 after Two Weeks of Incubation

as A Percentage of Viable Eggs From Weeks 6 7 and 8

0.940.950.960.970.980.991.001.011.02

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-40 

Means ± 2 Standard Errors for

Eggs Hatched as A Percentage of Number of Viable Eggs

from Weeks 7 and 8 after Two Weeks of Incubation

0.760.770.780.790.800.810.820.830.840.850.860.870.880.890.900.910.920.930.940.950.960.970.980.991.001.01

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-41 

Means ± 2 Standard Errors for

Number of 14-Day-Old Surviving Chicks

as A Percentage of Eggs Hatched

0.640.660.680.700.720.740.760.780.800.820.840.860.880.900.920.940.960.981.001.02

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-42 

Means ± 2 Standard Errors for

Eggs Hatched as A Percentage of

Number of Eggs Set from Weeks 7 and 8

0.640.660.680.700.720.740.760.780.800.820.840.860.880.900.920.940.960.981.001.02

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-43 

Means ± 2 Standard Errors for

Eggs Hatched as A Percentage of

Number of Fertile Eggs from Weeks 7 and 8

0.700.710.720.730.740.750.760.770.780.790.800.810.820.830.840.850.860.870.880.890.900.910.920.930.940.950.960.970.980.991.00

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-44 

Means ± 2 Standard Errors for

Number of 14-Day-Old Surviving Chicks as A Percentage

of Number of Eggs Set from Weeks 7 and 8

0.560.580.600.620.640.660.680.700.720.740.760.780.800.820.840.860.880.900.920.940.960.98

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-45 

Means ± 2 Standard Errors for

Eggs Hatched Per Hen Per Day

0.440.460.480.500.520.540.560.580.600.620.640.660.680.700.720.740.760.780.800.820.840.860.880.900.92

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-46 

Means ± 2 Standard Errors for

Number of 14-Day-Old Surviving Chicks Per Hen Per Day

0.440.460.480.500.520.540.560.580.600.620.640.660.680.700.720.740.760.780.800.820.840.860.880.90

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-47 

Means ± 2 Standard Errors for

Pen Mean Egg Shell Thickness (mm)

0.1960.1980.2000.2020.2040.2060.2080.2100.2120.2140.2160.2180.2200.2220.2240.2260.2280.2300.2320.2340.2360.2380.2400.242

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-48 

Means ± 2 Standard Errors for

Pen Mean Egg Content Weight - Albumin (g)

34567

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-49 

Means ± 2 Standard Errors for

Pen Mean Egg Content Weight - Yolk (g)

3.03.13.23.33.43.53.63.73.83.94.04.14.24.34.44.5

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-50 

Means ± 2 Standard Errors for

Pen Mean Egg Content Weight - Combined (g)

789

1011

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-51 

Means ± 2 Standard Errors for

Age in Days at First Foam - Males

333435363738394041424344454647484950

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-52 

Means ± 2 Standard Errors for

Age in Days at First Egg - Females

41424344454647484950515253

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-53 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 4 Weeks - Males

0.180.190.200.210.220.230.240.250.260.270.280.290.300.310.320.330.34

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-54 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 5 Weeks - Males

0.220.240.260.280.300.320.340.360.380.400.420.440.460.480.500.520.540.560.580.600.620.640.660.680.70

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-55 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 6 Weeks - Males

0.360.380.400.420.440.460.480.500.520.540.560.580.600.620.640.660.680.700.720.740.76

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-56 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 7 Weeks - Males

0.520.530.540.550.560.570.580.590.600.610.620.630.640.650.660.67

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-57 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 8 Weeks - Males

0.530.540.550.560.570.580.590.600.610.620.630.64

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-58 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 9 Weeks - Males

0.540.550.560.570.580.590.600.610.620.630.64

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-59 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 4 Weeks - Females

0.150.160.170.180.190.200.210.220.230.240.250.260.270.280.290.300.310.32

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-60 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 5 Weeks - Females

0.00.10.20.30.40.50.60.7

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-61 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 6 Weeks - Females

0.00.10.20.30.40.50.60.70.80.91.01.11.2

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-62 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 7 Weeks - Females

0.560.570.580.590.600.610.620.630.640.650.660.670.680.690.700.710.720.730.74

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-63 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 8 Weeks - Females

0.560.570.580.590.600.610.620.630.640.650.660.670.680.690.700.71

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-64 

Means ± 2 Standard Errors for

Cloacal Area (cm2) at 9 Weeks - Females

0.610.620.630.640.650.660.670.680.690.700.710.720.730.740.75

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-65 

Means ± 2 Standard Errors for

14-Day Old Left Thyroid Weight (mg)

4.34.44.54.64.74.84.95.05.15.25.3

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-66 

Means ± 2 Standard Errors for

14-Day Old Left Thyroid Weight (mg) - Males

0123456

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-67 

Means ± 2 Standard Errors for

14-Day Old Left Thyroid Weight (mg) - Females

1.51.61.71.81.92.02.12.22.32.42.52.62.72.82.93.03.13.23.33.4

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-68 

Means ± 2 Standard Errors for

Length of Time to First Neck Grab (sec) - Day 1

-40-30-20-10

0

102030405060708090

100110120130140

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-69 

Means ± 2 Standard Errors for

Length of Time to First Neck Grab (sec) - Day 2

-50-40-30-20-10

0

102030405060708090

100110120130

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-70 

Means ± 2 Standard Errors for

Length of Time to First Neck Grab (sec) - Day 3

-50-40-30-20-10

0

102030405060708090

100110120130140

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-71 

Means ± 2 Standard Errors for

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 -50 -40 -30 -20 -10

0

10

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-72 

Means ± 2 Standard Errors for

Length of Time to First Mount (sec) - Day 1

-30-20-10

0

102030405060708090

100110120130140150

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-73 

Means ± 2 Standard Errors for

Length of Time to First Mount (sec) - Day 2

-100

0

100200

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-74 

Means ± 2 Standard Errors for

Length of Time to First Mount (sec) - Day 3

-40-30-20-10

0

102030405060708090

100110120130140

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-75 

Means ± 2 Standard Errors for

Change in Length of Time to First Mount (sec) from Day 1 to Day 3

-140-130-120-110-100

-90-80-70-60-50-40-30-20-10

0

102030

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-76 

Means ± 2 Standard Errors for

Number of Mount Attempts (count) - Day 1

0123456789

1011121314

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-77 

Means ± 2 Standard Errors for

Number of Mount Attempts (count) - Day 2

0123456789

1011121314151617181920

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-78 

Means ± 2 Standard Errors for

Number of Mount Attempts (count) - Day 3

123456789

1011121314151617181920

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-79 

Means ± 2 Standard Errors for

Change in Number of Mount Attempts (count) from Day 1 to Day 3

-6-5-4-3-2-1

0123456789

10111213

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-80 

Means ± 2 Standard Errors for

Number of Cloacal Contacts (count) - Day 1

012345

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-81 

Means ± 2 Standard Errors for

Number of Cloacal Contacts (count) - Day 2

0123456789

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-82 

Means ± 2 Standard Errors for

Number of Cloacal Contacts (count) - Day 3

123456789

1011

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-83 

Means ± 2 Standard Errors for

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 -2-1

012345678

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-84 

Means ± 2 Standard Errors for

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1

-3-2-1

0123456789

101112

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-85 

Means ± 2 Standard Errors for

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2

-2-1

0123456789

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-86 

Means ± 2 Standard Errors for

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3

-1

0123456789

10

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-87 

Means ± 2 Standard Errors for

Change in Number of Crows (Cock) or Fluffs (Hen) (count) from Day 1 to Day 3 -8-7-6-5-4-3-2-1

01234

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-88 

Means ± 2 Standard Errors for

15 Day Embryo (ED15) Thyroid Weight (mg)

01234

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-89 

Means ± 2 Standard Errors for

Adult Left Thyroid Weight (mg) - Males

3456789

101112131415

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-90 

Means ± 2 Standard Errors for

Adult Left Thyroid Weight (mg) - Females

456789

1011121314151617181920

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-91 

Means ± 2 Standard Errors for

Ratio of Number of Males to Total Number of Offspring (%)

3040506070

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-92 

Means ± 2 Standard Errors for

Egg Yolk Estradiol (pg/g)

102030405060708090

100110120

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-93 

Means ± 2 Standard Errors for

Egg Yolk Testosterone (pg/g)

1000200030004000500060007000

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-94 

Means ± 2 Standard Errors for

Plasma Thyroid Hormone T3 - Adult Males (ng/mL)

0.30.40.50.60.70.80.91.01.11.21.31.4

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-95 

Means ± 2 Standard Errors for

Plasma Thyroid Hormone T3 - Adult Females (ng/mL)

0.40.50.60.70.80.91.01.11.2

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-96 

Means ± 2 Standard Errors for

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL)

01234

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-97 

Means ± 2 Standard Errors for

Plasma Thyroid Hormone T4 - Adult Males (ng/mL)

0123456789

10

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-98 

Means ± 2 Standard Errors for

Plasma Thyroid Hormone T4 - Adult Females (ng/mL)

0123456

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-99 

Means ± 2 Standard Errors for

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL)

0123456

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-100 

Means ± 2 Standard Errors for

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL)

0123456

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-101 

Means ± 2 Standard Errors for

Plasma Sex Hormone Estradiol - Adult Males (pg/mL)

0123456789

1011121314151617181920

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-102 

Means ± 2 Standard Errors for

Plasma Sex Hormone Estradiol - Adult Females (pg/mL)

0

10203040

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-103 

Means ± 2 Standard Errors for

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL)

23456789

10

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-104 

Means ± 2 Standard Errors for

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL)

56789

1011121314151617181920

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-105 

Means ± 2 Standard Errors for

Plasma Sex Hormone Testosterone - Adult Males (pg/mL)

0

1000200030004000

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-106 

Means ± 2 Standard Errors for

Plasma Sex Hormone Testosterone - Adult Females (pg/mL)

-100

0

100200300400500600700800900

100011001200

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-107 

Means ± 2 Standard Errors for

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL)

0

10203040506070

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
DRAFT 

Avian Control Data 

A-108 

Means ± 2 Standard Errors for

Plasma Sex Hormone Testosterone - 14-Day Old Females (pg/mL)

0

102030405060

ST SV WLI ALL ST SV WLI ALL ST 

Generation F0 Generation F1 G
Appendix B 

Figures for Avian Inter-Laboratory Analysis: Comparisons of Vinclozolin vs 

Control 

Means ± 2 Standard Errors Plots for Each Endpoint by Generation
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-1 

Adult Body Weight (g) Day 0 - Males (F0)

100120140160180200220240260

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-2 

Adult Body Weight (g) Day 0 - Males (F1)

80

100120140160180200220

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-3 

Adult Body Weight (g) Day 56 - Males (F0)

110130150170190210230250270

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-4 

Adult Body Weight (g) Day 56 - Males (F1)

110130150170190210230250270

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-5 

Adult Body Weight (g) Day 0 - Females (F0)

130140150160170180190200210220230240250260270280290300

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-6 

Adult Body Weight (g) Day 0 - Females (F1)

80

100120140160180200220240

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-7 

Adult Body Weight (g) Day 56 - Females (F0)

140160180200220240260280300320340

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-8 

Adult Body Weight (g) Day 56 - Females (F1)

140160180200220240260280300320340

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-9 

Mean Offspring Body Weight by Pen (g) Day 0 (F1)

6789

1011

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-10 

Mean Offspring Body Weight by Pen (g) Day 0 (F2)

6789

1011

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-11 

Mean Offspring Body Weight by Pen (g) Day 14 (F1)

405060708090

100110

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-12 

Mean Offspring Body Weight by Pen (g) Day 14 (F2)

405060708090

100110

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-13 

Adult Feed Consumption (g/bird/day) - Week 1 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-14 

Adult Feed Consumption (g/bird/day) - Week 1 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-15 

Adult Feed Consumption (g/bird/day) - Week 2 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-16 

Adult Feed Consumption (g/bird/day) - Week 2 (F1)

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-17 

Adult Feed Consumption (g/bird/day) - Week 3 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-18 

Adult Feed Consumption (g/bird/day) - Week 3 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-19 

Adult Feed Consumption (g/bird/day) - Week 4 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-20 

Adult Feed Consumption (g/bird/day) - Week 4 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-21 

Adult Feed Consumption (g/bird/day) - Week 5 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-22 

Adult Feed Consumption (g/bird/day) - Week 5 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-23 

Adult Feed Consumption (g/bird/day) - Week 6 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-24 

Adult Feed Consumption (g/bird/day) - Week 6 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-25 

Adult Feed Consumption (g/bird/day) - Week 7 (F0)

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-26 

Adult Feed Consumption (g/bird/day) - Week 7 (F1)

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-27 

Adult Feed Consumption (g/bird/day) - Week 8 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-28 

Adult Feed Consumption (g/bird/day) - Week 8 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-29 

Adult Feed Consumption (g/bird/day) - Week 9 (F0)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-30 

Adult Feed Consumption (g/bird/day) - Week 9 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-31 

Adult Feed Consumption (g/bird/day) - Week 10 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-32 

Adult Feed Consumption (g/bird/day) - Week 11 (F1)

10152025303540

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-33 

Eggs Laid (F0)

2025303540455055

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-34 

Eggs Laid (F1)

2025303540455055

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-35 

Eggs Crack (F0)

01234

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-36 

Eggs Crack (F1)

012345

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-37 

Eggs Abnormal (F0)

012345

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-38 

Eggs Abnormal (F1)

01234567

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-39 

Eggs Set From Weeks 7 and 8 (F0)

789

1011121314

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-40 

Eggs Set From Weeks 7 and 8 (F1)

789

1011121314

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-41 

Eggs Set From Weeks 6 7 and 8 (F0)

10121416182022

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-42 

Eggs Set From Weeks 6 7 and 8 (F1)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-43 

Eggs Fertile From Weeks 7 and 8 (F0)

789

1011121314

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-44 

Eggs Fertile From Weeks 7 and 8 (F1)

789

1011121314

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-45 

Eggs Fertile From Weeks 6 7 and 8 (F0)

101214161820

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-46 

Eggs Fertile From Weeks 6 7 and 8 (F1)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-47 

Eggs Viable From Weeks 7 and 8 (F0)

789

1011121314

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-48 

Eggs Viable From Weeks 7 and 8 (F1)

79

111315171921

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-49 

Eggs Viable From Weeks 6 7 and 8 (F0)

101214161820

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-50 

Eggs Viable From Weeks 6 7 and 8 (F1)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-51 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation (F0)

789

1011121314

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-52 

Viable Eggs From Weeks 7 and 8 after Two Weeks of Incubation (F1)

79

111315171921

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-53 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation (F0)

79

111315171921

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-54 

Viable Eggs From Weeks 6 7 and 8 after Two Weeks of Incubation (F1)

79

111315171921

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-55 

Eggs Hatched (F0)

5

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-56 

Eggs Hatched (F1)

5

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-57 

Number of 14-Day-Old Surviving Chicks (F0)

5

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-58 

Number of 14-Day-Old Surviving Chicks (F1)

5

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-59 

Eggs Laid Per Hen Per Day (F0)

0.50.60.70.80.91.01.1

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-60 

Eggs Laid Per Hen Per Day (F1)

0.50.60.70.80.91.01.1

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-61 

Cracked Eggs as A Percentage of Eggs Laid (F0)

0.000.010.020.030.040.050.060.070.080.090.10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-62 

Cracked Eggs as A Percentage of Eggs Laid (F1)

0.000.010.020.030.040.050.060.070.080.090.100.110.12

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-63 

Abnormal Eggs as A Percentage of Eggs Laid (F0)

0.000.050.100.150.200.25

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-64 

Abnormal Eggs as A Percentage of Eggs Laid (F1)

0.000.050.100.150.200.25

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-65 

Fertile Eggs as A Percentage From Weeks 7 and 8 (F0)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-66 

Fertile Eggs as A PercentageFrom Weeks 7 and 8 (F1)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-67 

Fertile Eggs as A Percent From Weeks 6 7 and 8 (F0)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-68 

Fertile Eggs as A Percent From Weeks 6 7 and 8 (F1)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-69 

Viable Eggs as A Percent From Weeks 7 and 8 (F0)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-70 

Viable Eggs as A Percent From Weeks 7 and 8 (F1)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-71 

Viable Eggs as A Percent From Weeks 6 7 and 8 (F0)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-72 

Viable Eggs as A Percent From Weeks 6 7 and 8 (F1)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-73 

Viable Eggs (7-8) as A Percent From Weeks 7 and 8 (F0)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-74 

Viable Eggs (7-8) as A Percent From Weeks 7 and 8 (F1)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-75 

Viable Eggs (6-8) as A Percent From Weeks 6 7 and 8 (F0)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-76 

Viable Eggs (6-8) as A Percent From Weeks 6 7 and 8 (F1)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-77 

Eggs Hatched as A Percent from 7-8 after Incub (F0)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-78 

Eggs Hatched as A Percent from 7-8 after Incub (F1)

0.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-79 

14+ Surviving Chicks as A Percent of Eggs Hatched (F0)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-80 

14+ Surviving Chicks as A Percent of Eggs Hatched (F1)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-81 

Eggs Hatched as A Percent from Weeks 7 and 8 (F0)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-82 

Eggs Hatched as A Percent from Weeks 7 and 8 (F1)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-83 

Eggs Hatched as A Percentage from Weeks 7 and 8 (F0)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-84 

Eggs Hatched as A Percentage from Weeks 7 and 8 (F1)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-85 

Number of 14+ Surviving Chicks as A Percentfrom Weeks 7 and 8 (F0) 0.0 0.2 0.4 0.6 0.8 1.0 1.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-86 

Number of 14+ Surviving Chicks as A Percentfrom Weeks 7 and 8 (F1) 0.0 0.2 0.4 0.6 0.8 1.0 1.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-87 

Eggs Hatched Per Hen Per Day (F0)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-88 

Eggs Hatched Per Hen Per Day (F1)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-89 

Number of 14+ Surviving Chicks Per Hen Per Day (F0)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-90 

Number of 14+ Surviving Chicks Per Hen Per Day (F1)

0.00.20.40.60.81.01.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-91 

Pen Mean Egg Shell Thickness (mm) (F0)

0.190.200.210.220.230.240.25

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-92 

Pen Mean Egg Shell Thickness (mm) (F1)

0.190.200.210.220.230.240.25

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-93 

Pen Mean Egg Content Weight - Albumin (g) (F0)

345678

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-94 

Pen Mean Egg Content Weight - Albumin (g) (F1)

345678

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-95 

Pen Mean Egg Content Weight - Yolk (g) (F0)

345

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-96 

Pen Mean Egg Content Weight - Yolk (g) (F1)

345

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-97 

Pen Mean Egg Content Weight - Combined (g) (F0)

789

101112

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-98 

Pen Mean Egg Content Weight - Combined (g) (F1)

789

101112

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-99 

Age in Days at First Foam - Males (F1)

30354045505560

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-100 

Age in Days at First Egg - Females (F1)

30354045505560

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-101 

Cloacal Area (cm2) at 4 Weeks - Males (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-102 

Cloacal Area (cm2) at 5 Weeks - Males (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-103 

Cloacal Area (cm2) at 6 Weeks - Males (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-104 

Cloacal Area (cm2) at 7 Weeks - Males (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-105 

Cloacal Area (cm2) at 8 Weeks - Males (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-106 

Cloacal Area (cm2) at 9 Weeks - Males (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-107 

Cloacal Area (cm2) at 4 Weeks - Females (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-108 

Cloacal Area (cm2) at 5 Weeks - Females (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-109 

Cloacal Area (cm2) at 6 Weeks - Females (F1)

0.00.10.20.30.40.50.60.70.80.91.01.11.2

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-110 

Cloacal Area (cm2) at 7 Weeks - Females (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-111 

Cloacal Area (cm2) at 8 Weeks - Females (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-112 

Cloacal Area (cm2) at 9 Weeks - Females (F1)

0.00.10.20.30.40.50.60.70.80.91.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-113 

Length of Time to First Neck Grab (sec) - Day 1 (F1)

0

50

100150200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-114 

Length of Time to First Neck Grab (sec) - Day 1 (F2)

0

50

100150200250300

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-115 

Length of Time to First Neck Grab (sec) - Day 2 (F1)

0

20406080

100120140160180200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-116 

Length of Time to First Neck Grab (sec) - Day 2 (F2)

0

20406080

100120140160180200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-117 

Length of Time to First Neck Grab (sec) - Day 3 (F1)

0

20406080

100120140160180200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-118 

Length of Time to First Neck Grab (sec) - Day 3 (F2)

0

20406080

100120140160180200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-119 

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 (F1) -200 -150 -100 -50

0

50

100

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-120 

Change in Length of Time to First Neck Grab (sec) from Day 1 to Day 3 (F2) -200 -150 -100 -50

0

50

100

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-121 

Length of Time to First Mount (sec) - Day 1 (F1)

0

50

100150200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-122 

Length of Time to First Mount (sec) - Day 1 (F2)

0

50

100150200250300

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-123 

Length of Time to First Mount (sec) - Day 2 (F1)

0

50

100150200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-124 

Length of Time to First Mount (sec) - Day 2 (F2)

0

50

100150200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-125 

Length of Time to First Mount (sec) - Day 3 (F1)

0

50

100150200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-126 

Length of Time to First Mount (sec) - Day 3 (F2)

0

50

100150200

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-127 

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 (F1) -200 -150 -100 -50

0

50

100150

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-128 

Change in Length of Time to First Mount (sec) from Day 1 to Day 3 (F2) -200 -150 -100 -50

0

50

100

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-129 

Number of Mount Attempts (count) - Day 1 (F1)

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-130 

Number of Mount Attempts (count) - Day 1 (F2)

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-131 

Number of Mount Attempts (count) - Day 2 (F1)

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-132 

Number of Mount Attempts (count) - Day 2 (F2)

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-133 

Number of Mount Attempts (count) - Day 3 (F1)

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-134 

Number of Mount Attempts (count) - Day 3 (F2)

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-135 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 (F1) -20 -15 -10-5

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-136 

Change in Number of Mount Attempts (count) from Day 1 to Day 3 (F2) -20 -15 -10-5

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-137 

Number of Cloacal Contacts (count) - Day 1 (F1)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-138 

Number of Cloacal Contacts (count) - Day 1 (F2)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-139 

Number of Cloacal Contacts (count) - Day 2 (F1)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-140 

Number of Cloacal Contacts (count) - Day 2 (F2)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-141 

Number of Cloacal Contacts (count) - Day 3 (F1)

02468

1012

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-142 

Number of Cloacal Contacts (count) - Day 3 (F2)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-143 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 (F1) -5

05

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-144 

Change in Number of Cloacal Contacts (count) from Day 1 to Day 3 (F2) -5

05

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-145 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 (F1)

02468

101214

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-146 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 1 (F2)

02468

101214

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-147 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 (F1)

02468

101214

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-148 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 2 (F2)

02468

101214

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-149 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 (F1)

02468

101214

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-150 

Number of Crows (Cock) or Fluffs (Hen) (count) - Day 3 (F2)

02468

101214

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-151 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) (F1)

-10

-5

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-152 

Change in Number of Crows (Cock) or Fluffs (Hen) (count) (F2)

-10

-5

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-153 

15 Day Embryo (ED15) Thyroid Weight (mg) (F1)

01234

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-154 

15 Day Embryo (ED15) Thyroid Weight (mg) (F2)

01234

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contro3-160 pp5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-155 

Adult Left Thyroid Weight (mg) - Males (F0)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-156 

Adult Left Thyroid Weight (mg) - Males (F1)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-157 

Adult Left Thyroid Weight (mg) - Females (F0)

05

1015202530

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-158 

Adult Left Thyroid Weight (mg) - Females (F1)

05

1015202530

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-159 

14-Day Old Left Thyroid Weight (mg) (F2)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-160 

14-Day Old Left Thyroid Weight (mg) - Males (F2)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-161 

14-Day Old Left Thyroid Weight (mg) - Females (F2)

02468

10

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-162 

Ratio of Number of Males to Total Number of Offspring (%) (F1)

3040506070

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-163 

Ratio of Number of Males to Total Number of Offspring (%) (F2)

3040506070

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-164 

Egg Yolk Estradiol (pg/g) (F0)

0

20406080

100120

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-165 

Egg Yolk Estradiol (pg/g) (F1)

0

20406080

100120

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-166 

Egg Yolk Testosterone (pg/g) (F0)

0

2000400060008000

10000

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Cont3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-167 

Egg Yolk Testosterone (pg/g) (F1)

0

2000400060008000

10000

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Cont3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-168 

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) (F0)

0.00.51.01.52.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-169 

Plasma Thyroid Hormone T3 - Adult Males (ng/mL) (F1)

0.00.51.01.52.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-170 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) (F0)

0.00.51.01.52.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-171 

Plasma Thyroid Hormone T3 - Adult Females (ng/mL) (F1)

0.00.51.01.52.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-172 

Plasma Thyroid Hormone T3 - 14-Day Old Males (ng/mL) (F2)

0.00.51.01.52.02.5

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-173 

Plasma Thyroid Hormone T3 - 14-Day Old Females (ng/mL) (F2)

0.00.51.01.52.02.53.03.54.0

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-174 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) (F0)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-175 

Plasma Thyroid Hormone T4 - Adult Males (ng/mL) (F1)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-176 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) (F0)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-177 

Plasma Thyroid Hormone T4 - Adult Females (ng/mL) (F1)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-178 

Plasma Thyroid Hormone T4 - 14-Day Old Males (ng/mL) (F2)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-179 

Plasma Thyroid Hormone T4 - 14-Day Old Females (ng/mL) (F2)

05

1015

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-180 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) (F0)

05

101520

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-181 

Plasma Sex Hormone Estradiol - Adult Males (pg/mL) (F1)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-182 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) (F0)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-183 

Plasma Sex Hormone Estradiol - Adult Females (pg/mL) (F1)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-184 

Plasma Sex Hormone Estradiol - 14-Day Old Males (pg/mL) (F2)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-185 

Plasma Sex Hormone Estradiol - 14-Day Old Females (pg/mL) (F2)

0

1020304050

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 2 

A

Group & Lab

1-Contr3-160 p5-1000 p
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-186 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) (F0)

0

10002000300040005000600070008000

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Cont3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-187 

Plasma Sex Hormone Testosterone - Adult Males (pg/mL) (F1)

0

10002000300040005000600070008000

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Cont3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-188 

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) (F0)

0

500

100015002000

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Cont3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-189 

Plasma Sex Hormone Testosterone - Adult Females (pg/mL) (F1)

0

500

100015002000

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Cont3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-190 

Plasma Sex Hormone Testosterone - 14-Day Old Males (pg/mL) (F2)

0

20406080

100

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000 
DRAFT 

Avian Interlab Analysis: Comparisons of Vinclozolin and Control 

B-191 

Plasma Sex Hormone Testosterone - 14-Day Old Females (pg/mL) (F2)

0

20406080

100

1 2 3 4 5

ST

1 2 3 4 5

SV

1 2 3 4 5

WLI

1 

A

Group & Lab

1-Contr3-160 p5-1000