### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates

-Daphnia sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### Data Requirement: PMRA Data Code EPA DP Barcode OECD Data Point EPAMRID EPA Guideline {

## ..........} 

### 361296,358877,358880,358882,358885 { } 

### 47643007 850.1300 

### Test material: Commonname: Chemical name: 

### IN L5300-78 

### Purity: 79.8% ai Tribenuron-methyl (ai) IUPAC: methyl 2-[4-methoxy-6-methyl-1 ,3,5-triazin-2-yl(methyl) carbamoylsulfamoyI]benzoate CAS name: methyI2-[[[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl) methylamino]carbonyl]amino]-sulfonyl]benzoate CAS No.: 101200-48-0 (ai) Synonyms: Haskell No. 17,738-03 

### Primary Reviewer: Christie E. Padova StaffScientist, Dynamac Corporation 

### Secondary Reviewer: Teri S. Myers Senior Scientist, Cambridge Environmental Inc. 

### Reviewer: ChristinaWendel USEPA/OCSPP/OPP/ERBII/Biologist 

### Reference/Submission No.: {. } Signature: 

# t.**..p~** Date: 06/16/2011 

### Signature:

## ~'Sll~ Date: 07/13/2011

# Signature:~ 

# wA Date: 12/06/2011


### Company Code Active Code Use Site Category: EPA PC Code { } [For PMRA] { } [For PMRA] { } [For PMRA] 128887 

### Date Evaluation Completed: 12/06/2011 

### CITATION: Hutton, D.G. 1989. Chronic Toxicity of DPX-L5300-78 to Daphnia magna. Unpublished study performed by E.!. du Pont de Nemours and Company, Inc., Newark, DE, and sponsored by E.!. du Pont de Nemours and Company, Wilmington, DE. Laboratory Study No. 555-89. Study initiatedJuly 14,1989 and completed October 18, 1989.

Page 1 of30

1•••

# 1•••11 2086465

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates

-

### Daphnia sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### EXECUTIVE SUMMARY: The 21-day-chronic toxicity ofDPX-L5300-78 (a formulation ofTribenuron-methyl, 79.8% ai) to Daphnia magna 

### was studied under static-renewal conditions. Daphnids were exposed to DPX-L5300-78 at nominal concentrations of

# °(negative control), 31, 62, 125, 250, 500, and 1000 mg/L. Mean-measured concentrations were <3.13 «LOQ, control), 28, 54, 110, 220, 400, and 790 mg ai/L. The reviewer concluded that a NOAEC could not be determined in this study due to significant (p<0.05) treatment-related delays in time to first brood release at the 28, 54, and 110 mg ai/L levels and significant (p<0.05) treatment-dependentreductions in adult length at all levels tested. Additionally, offspring production (both total and young/day per surviving adult) was significantly reduced (p<0.05) at the 54, 110, and 220 mg ai/L levels. Immobility of adults was significantly (p<0.05) affected at the 220, 400, and 790 mg ai/L levels, with complete immobility occurring at the 400 and 790 mg ai/L levels. The 21-day ECso(with 95% c.1.) 

### for immobilization was 216 (184-252) mg ai/L. The 21-day ECso based on reproduction parameters was 100 (63-170) mg ai/L for total offspring and 170 (81-380) mg ai/L for offspring produced/dayper surviving adult. Following 21 days ofexposure, survival averaged 90% in the control group, and 98, 90, 80, 72, 0, and 0% in the mean-measured 28, 54, 110, 220, 400, and 790 mg ai/L treatment levels, respectively. The differences were statistically-reduced (p<0.05) compared to the negative control at the 220, 400 and 790 mg ai/L levels. The mean time to first brood release was 11.4 days for the negative control, and 13.0, 13.3, 14.4, and 12.7 days for the mean-measured 28, 54, 110, and 220 treatment levels, respectively (no offspring were produced at the 400 and 790 mg ai/L levels). Delays were statistically-significant (p<0.05) compared to the control at the 28, 54 and 110 mg ai/L levels. For the control, and mean-measured 28, 54, 110, and 220 mg ai/L levels, the total numbers of young produced were 383,333,274, 135, and 150, respectively, and the number ofoffspring per surviving parent averaged 96,85,74,41, and 52, respectively. For both parameters, differences were statistically-significant (p<0.05) compared to the control at the 54, 110, and 220 mg ai/L levels. No males, ephippia, or immobilized young were observed in the control group or any treatment level during the study, and no eggs were observed in the bottom ofany test vessel. Growth was measured as length at study termination. Mean lengths were 3.7, 3.7, 3.6, 3.2, and 3.1 mm for the control and mean-measured 28, 54, 110, and 220 mg ai/L levels, respectively. Reductions for this parameter followed a dose-dependent pattern and differences were statistically-significant (p<0.05) compared to the control at all treated levels. Although this study is scientifically sound itdoes not 

### satisfy the guideline requirement for a chronic toxicity study with freshwater invertebrates due to the inability to establish a definitive NOAEC «28 mg ai/L, the lowest test concentration). Based on this, the study is classified as 

### Supplemental and is not repairable. Page 2 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of 

### Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp, PMRA SubmissionNumber {........} EPA MRID No. 47643007 

### Results Synopsis Test Organism Age (eg. 151

### instar): First instar, <24 hours old Test Type (Flow-through, Static, Static Renewal): Static-renewal

21-day ECso(mortality/immobility): 216 mg ai/L 21-day ECso(reproduction): 100 mg ai/L Slope: 1.42±0.331 NOAEC: <28 mg ai/L LOAEC: 28 mg ai/L 95% C.I.: 184-252 mg ai/L (moving average method) 95% C.l.: 63-170 mg ai/L

Endpoints affected: Survival, time to first brood release, total young produced, young/day per surviving adult, and growth (length) Most sensitive endpoint(s): Time to first brood release and growth (length) I. 

### MATERIALS AND METHODS GUIDELINE FOLLOWED: This study was conducted following guidelines outlined in OECD Guideline No. 202 (1984) and U.S. EPA Standard EvaluationProcedure, Daphnia magna 

### Life-Cycle (21-Day Renewal) Chronic Toxicity Test" (1986). Deviations from OPPTS 850.1300 (draft, 

### 1996) guidance included: 1. ANOAEC couldnot be determined in this study due to treatment-related effects on first day ofbrood production and adult length at the lowest treatment level. 2. The source ofthe dilution water used in the toxicity test was filtered well water that had been previously used for fathead minnow culturing in a flow-through system within the test laboratory, and filtered through a 0.8f.lm filter prior to use in the daphnid toxicity study. The water was not tested for the presence ofammonia, therefore it is not known if that may have had a negative impact on the health ofthe test organisms. 3. The type and quantity offood offered at each renewal period was not reported. These deviations do 

### affect the acceptability ofthe study. 

### COMPLIANCE:
A. MATERIALS: 1. 

### Test Material: Signed and dated GLP (1989), Quality Assurance (1989), and Data Confidentiality claims (2008) statements were provided.

DPX-L5300-78 (formulation ofTribenuron-methyl) Page 3 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates

-Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### Description: Not reported 

### Lot No./Batch No.: Not reported 

### Purity: 79.8% ai 

### Stability ofcompound under test conditions: The stability of

### Tribenuron-methyl was verified analytically at all levels weekly throughout the study. For all levels, replicate results were within ±21%. 

### Storage conditions of test chemicals: Not reported 

### th I 

### fT ·b I lySICOC 

### emrca 

### properties 0 rr 

### enuron-me y. Parameter Values Comments Watersolubility at 20°C Not reported 

### Vapor pressure Not reported 

### UVabsorption Not reported 

### pKa Not reported 

### Kow Not reported 

### Ph . h

(GECD recommends watersolubility, stability in waterandlight, pKa, Pow, andvapor pressure of test compound) 

### 2. Test Organism: Species: Daphnia magna, first instar, <24 hours old 

### EPA andGECD recommendDaphnia magna 

### Age ofthe parental stock: 21 days old 

### EPA recommends thatyoung daphnids**~2** 

### 4 

### hours old from a separateparentalculture be used 

### Source: Haskell Laboratory bred culture 

### EPA requires all test organisms mustbeproduced from laboratory rearedculture that has been maintained for at least 21 

### days at test conditions in dilution water with renewal of the culture medium at least three timesperweek. Page 4 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### B. STUDY DESIGN: 1. 

### Experimental Conditions

a. Range-finding Study: Nominal test levels for the definitive study were based upon acute toxicity data determined prior to study initiation. The acute, static ECso 

### (immobilization) was>1000 mg/L for unfed Daphnia magna. 

### b. Definitive Study 

### Table 1: 

### Experimental Parameters Remarks Parameter

Parental acclimation: Period: Conditions: (same as test or not) Feeding: Health (any mortality observed):

Test condition: static renewal/flow-through: Type ofdilution system- for flow through method.
Renewal rate for static renewal 

### Details

21 days Same as test Trout chow (Glencoe) and yeast (Fleischmann's) No sickness, injuries, or abnormalities were noted in the test organisms prior to initiating the test.

Static-renewal
N/A Three times per week



### Page 5 of30 Criteria Daphnia were cultured in 2-L beakers containing ca. 1.8 L of water and 25 daphniaper beaker. EPA recommends thatprior to testing, daphnids that are at least 10-12 days old(those that have hadat least one brood) shouldbe separated from the culture, put in separate containerand maintained for at least 21 days to insure thatgoodhealth conditions arepresent The parent Daphnia were transferred into fresh test solutions containing food three times per week. (EPA requires consistent flow rate of 5-10 vol/24 hours, meter systems calibratedbefore study andcheckedtwice daily during testperiod)

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) 

### to FreshwaterInvertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007
Remarks Parameter Aeration, if any

Duration ofthe test

Test vessel Material: (glass/stainless steel) Size (for growth and reproduction/survival test): Fill volume:

Source ofdilution water 

### Details

No supplemental aeration was provided.
21 days

Glass
250 mL (6.1-cm diameter) Ca. 200 mL (6.8-cm depth)

The dilution waterused for testing was filtered fish tank water (laboratory well water that had been used for fathead minnow culturing in a flow-through system, then filtered through a 0.8-/lm filter). Water was originally obtained from a 350-foot encased (in bedrock), and aerated and filtered (10 um) prior to use. 

### Criteria

EPA recommends test chambers shouldnot be aerated

Recommendedduration is 21 days.

1. RecommendedMaterial: Glass, No. 316 stainless steel, or per fluorocarbonplastics 2. RecommendedSize: 250 ml with 200 mlfill volume; 100 ml with 80 mlfill volume GECD guideline recommends that parentanimals be maintained individually; onepervessel, with 50

- 100 ml of medium in each vessel. Results from periodic analysis of the well waterwere provided; water quality measurements of the dilution water were not taken after use in the fish culturing. The use ofold fish culture water as the dilution water is a notable deviation. It is unknown if the water contained ammonia as measurements were not taken. Therefore it is not known if ammonia was present in the dilution water used in the Daphniatoxicity test; or if it may have had a negative impact on the health ofthe test organisms. IL- --l.- ----'

------------- 

### Page 6 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007


### Remarks Parameter Details

- - - - - - - - - ----------- - - - - - - --- ----------- 

### Criteria Recommendedsource of dilution water includes unpollutedwell or springwater that has been tested for contaminants, orappropriate reconstitutedwater (seeASTM for details). Water parameters: DO was ::::60% (equivalent to Hardness 76 ± 3 mg/L as CaC03 ::::5.5 mg/L at 20°C; reviewercalculated) throughoutthe study. pH Fresh soln.: 7.1 to 8.6 Aged soln.: 7.1 to 8.1 Alkalinity was 83 ± 3 mg/L as CaC03, and conductivity was Dissolved oxygen Fresh soln.: 8.3 to 8.8 mg/L 165 ± 8 umhos/cm. Aged soln.: 5.5 to 8.3 mg/L Raw waterchemistry data were Temperature 19.5 to 20.l°C not provided. However, the chemical characteristics ofthe Total Organic Carbon Not reported Haskell Laboratory Well Water Particulate matter Not reported are as follows: TOC: <1.5 mg/L; Inorganic analytes Not reported COD: <17mg/L; Solids, total Pesticides Not reported suspended (TSS): <4 mg/L; Chlorine Notreported Pesticides: None detected (but detection level was not reported); Frequency ofmeasurements Dissolved oxygen (DO) was Chloride: 3.7 mg/L

- ---- - - - - - - - - - - - - - - - - - ------------------

Page 

### 7 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates

- Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### Remarks Parameter Details

--- ------------------------------------- 

### Criteria measured at each renewal 

### Recommendedhardness: 160 to period in one old and one 180 mg/L as CaCG3; GECD new test solution from all recommends>140 mg/L as levels. The pH was CaCG3 measured at each renewal 

### Recommended pH: 7.6 to 8.0pH period in one old and one shouldnot deviate by more than new test solution from the 1.0 unitformore than 48 hours. control, 31 (low), 250 GECD recommends thatpH (medium), and 1000 (high) range be 6 - 9 anddoes not vary treatment levels. The more than 1.5 units in anyone temperature was test. continuously monitored (not 

### Recommendeddissolved further specified) and 

### oxygen: renewalshouldnot manually checked each drop below 50%formore than workday. The hardness, 48 hours. total alkalinity, and Recommended flow-through: conductivity ofthe control ?-60% throughouttest. dilution waterwere 

### Recommendedtemperature: measured once weekly 20°C± 2°e.; shouldnot deviate during the test. from 20°Cby more than 5°Cfor more than 48 hours. GECD recommends a range of18 - 22°C; temperature shouldnot vary more than ± 2°C GECD guideline recommends that total organic carbon <2 mg/L

Page 

### 8 of

### 30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates

-Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### Remarks Parameter Details

---------------------------------------- 

### Criteria Number ofreplicates: There were six toxicant levels For growth and reproduction: 10replicates per level spaced by a separation factor of 2. For survival test: (same) ---- ---- ------ ---- -- -- -- ---------- -- ---- Number of replicates should include a control(s) andat least 5 test concentrations; dilution factor shouldnot be greaterthan 50%. DECD recommends that at least 5 test concentrations be used in a geometric series with a separationfactor not exceeding 3.2. Number oforganisms: Study design follows that For growth and reproduction: Four organisms per replicate described in OPPTS guidance for a flow-through study. All For survival test: (same) replicates were observed for survival, reproduction, and growth.

---------------------------------------- Recommendednumberof organisms include 22 daphnids/test concentration; 7 test chambers shouldcontain 1 daphnideach, and3 test chambers contain 5 daphnids each. DECD recommends holding a minimum of 10 daphnids individually for static tests. Forflow-through tests, 40 animals shouldbe dividedinto 4 groups of 10 animals at each test concentration.

Page 

### 9 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA Submission Number { } EPA MRID No. 47643007 

### Remarks Parameter Details

----------------------------------------- 

### Criteria Treatment Concentrations: It was not reported ifthe nominal nominal: 

# ° (negative control), 31, 62, concentrations were corrected for 125, 250, 500, and 100O purity ofthe test substance. mg/L Actual concentrations of mean-measured: <3.13 «LOQ, control), 28, Tribenuron-methyl were 54, 110, 220, 400, and 790 determined in fresh solutions (all mg ai/L levels) on Days 0, 7, and 14 and in aged ('old') solutions (all levels) on Days 7, 14, and 2l. Replicate results were generally maintained within 20% for all levels, with one exception: analytical variation was 21% at the nominal 62 mg/L level. Mean-measured concentrations were rounded to two significant figures by the study authors. Test organisms were transferred to new test solutions three times a week; however, concentrations were measured on day 0, 7, and 14 only. Solvent (type, percentage, ifused) N/A

-------------------- -------------------- Solventconcentration shouldnot exceed0.5 ml/Lforstatic tests or 0.1 mill forflow-through tests. Recommendedsolvents include dimethylformamide, triethylene glycol, methanol, acetone and ethanol. GEeDrecommends**~0.1** mL/l of solvent.

Page 10 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### Remarks Parameter Details
-------- - - - - - - - - - - --- ---------------- - -- 

### Criteria Lighting 16-hours light, 8-hours dark; light intensity was ca. 560

---------------------------------------- lux (not further specified) Recommendedphotoperiodis 16 hours light and

### 8 hours of dark. Recovery ofchemical: 75 to 101% ofnominal Reviewer-calculated from replicate results oftest solutions. Frequency ofmeasurement: Days 0, 7, 14, and 21 LaD: Not reported LOQ: 3.13 mg ai/L Positive control {if used, indicate Nat assessed the chemical and concentrations} Otherparameters, ifany Daphnia were provided food Trout chow (Glencoe) and yeast

Feeding: intheir freshly-prepared test (Fleischmann's) were fed to the solutions at each renewal daphnids during culturing; it is interval; however, the type unclear if the same food was and quantity were not used during the definitive test. reported. 

### 2. Observations: Table 2: Observations Remarks Parameters Details

----------------------------------------------- 

### Criteria Data endpoints measured - Survival (list) - Days to first brood

- ------------------------------ -- - --- - - -------- - Total no. young produced Recommendedendpoints measured: - No. young/surviving adult

- Survival of first-generation daphnids, - Growth (terminal length)

-Number of youngproducedper female, - Dry weight (required) and length (optional) of each firstgeneration daphnidalive at the endof the test,

- Observations of other effects or clinicalsigns. 

### Page 11 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007


### Remarks Parameters Details

------- ------ - ----------------- ------- ----- 

### Criteria Observation intervals Survival and reproduction were observed at each renewal period (three times per week). Growth was determined at Day 21 (test termination). Were raw data included? Yes (biological data) Otherobservations, if any None assessed.



### II. RESULTS AND DISCUSSION A. MORTALITY: Following 21 days ofexposure, survival averaged 90% in the control group, and 98, 90, 80, 72,0, and 0% in the mean-measured 32,56, 120,250,480, and 940 mg ai/L treatment levels, respectively. The study author reported that differences were statistically-reduced (p<0.05) compared to the negative control at the 400 and 790 mg ai/L levels. The 21-Day ECso

### for immobilization (with 95% C.l.) was reported by the study author to be 230 (160 to 350) mg ai/L. The NOAEC for survival was reported to be 220 mg ai/L. 

### Table 3: Effect ofDPX-L5300-78 (formulation ofTribenuron-methyl) on Survival,

R d f 

### d G 

### th fD h .



### epro uc ron, 

### an row 0 

### ap. nta sp. 

### Treatment Mean-Measured Survival, % 

### Days to First No. Young Mean

(and Nominal) No. Young Cone. Day 21 

### Brood per Parent I 

### Length, mm
I 

### mglL Control 90 11.4 383 96 3.7 (dilution water only) 28 (31) 98 13.0 333 85 3.7 54 (62) 90 13.3* 274* 74 3.6* 110 (125) 80 14.4* 135* 41* 3.2* Page 12of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates

-

### Daphnia sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 

### Treatment Mean-Measured Survival, % 

### Days to First No. Young Mean

(and Nominal) No. Young Cone. Day 21 Brood perParent Length, mm mglL 220 (250) 72 12.7 150* 52* 3.1* 400 (500) 0*



### --- 0* 0*



### --- 790 (1000) 0*


## --- 0* 0*



### --- NOAEC, mg ai/L 220 28 28 28 LOAEC, mg ai/L 400 54 54 54 * Significantly different (p<0.05) comparedto the control using Dunnett's Test. 

### B. EFFECTS ON REPRODUCTION AND GROWTH: The mean time to first broodrelease was 11.4 days for the negative control, and 13.0, 13.3, 14.4, and 12.7 days for the mean-measured 28,54, 110, and 220 treatment levels, respectively (no offspring were produced at the 400 and 790 mg ai/L levels). Differences were statistically-significant (p<0.05) comparedto the control at the 54 and 110 mg ai/L levels. Although no significant difference was indicated for the 220 mg ai/L level, the study author consideredthe statistical differences observed at the 54 and 110 levels to be treatmentrelated. Thus, the NOAEC for time to first brood release was reported to be 28 mg ai/L. For the control, and mean-measured 28, 54, 110, and 220 mg ai/L levels, the total numbers of young produced were 383, 333, 274, 135, and 150, respectively, and the number ofoffspring per surviving parent averaged 96,85, 74, 41, and 52, respectively. For total offspring, differences were statistically-significant (p<0.05) comparedto the control at the 54, 110, and 220 mg ai/L levels. When the data were corrected for surviving parent, differences remained statistically-significantfor the study author's analysis at only the 110 and 220 mg ai/L levels. Based upon total offspring (the more sensitive ofthe two reproductive endpoints), the study authors reported that the NOAEC for offspring production was 28 mg ai/L. It

### was reported that the 21-Day ECso

### for reproduction (with 95% C.I.) was 230 (160 to 350) mg ai/L. No males, ephippia, or immobilized young were observed in the control group or any treatment level during the study, and no eggs were observed in the bottom ofany test vessel. Growth was measured as length (mm) at study termination. Mean lengths were 3.7, 3.7, 3.6, 3., and 3.1 mm for the control and mean-measured 28,54, 110, and 220 mg ai/L levels, respectively. Differences were statistically-significant (p<0.05) for the study author's analysis compared to the control at the 54, 110, and 220 mg ai/L levels, respectively. The Page 13 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 NOAEC for growth was reported to be 28 mg ai/L. C. 

### REPORTED STATISTICS: Data that were statistically analyzed included organism survival, time to first brood release, total number ofoffspring, number ofoffspring produced per surviving adult and total body length. Survival data were analyzed using Fisher's Exact test and the Cochran-Armitage trend test. Reproductive and growth endpoints were analyzed using aone-way analysis of variance (ANOVA) followed by Dunnett's procedure. The 21-day ECso for immobilization (and presumably reproduction) was calculated by Probit analysis. All results were reported in terms ofmean-measured concentrations. Parental Survival ECso: 230 mg ai/L Probit slope: Not reported NOAEC: 220 mg ai/L LOAEC: 400 mg ai/L Time to First Brood NOAEC: 28 mg ai/L LOAEC: 54 mg ai/L Total offspring NOAEC: 28 mg ai/L LOAEC: 54 mg ai/L Offspring/surviving adult ECso: 230 mg ai/L Probit slope: Not reported NOAEC: 54 mg ai/L LOAEC: 110 mg ai/L Total Length NOAEC: 28 mg ai/L LOAEC: 54 mg ai/L 95% C.l.: 160 to 350 mg ai/L 95% C.l.: Not reported

95% C.l.: 160 to 350 mg ai/L 95% C.L: Not reported

D. VERIFICATION OF STATISTICAL RESULTS: Statistical Method(s): The reviewer determinedthe NOAEC for immobility using Fisher's Exact Test. Reproduction (time to first brood, total offspring, offspring produced/dayper surviving adult) and growth (length) endpoint data were determined to be normally distributed using the Shapiro-Wilk's or Chi-square test and variances were confirmed to be Page 14 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp, PMRA SubmissionNumber { } EPA MRID No. 47643007 homogeneous using Levene's test. The NOAEC and LOAEC for these endpoints were determinedusing ANOVA, followed by Bonferroni's, Dunnett's or William's test. These analyses were conducted using Toxstat 3.5. The reviewerdeterminedthe 21-day ECsofor reproductive endpoints (total offspring production and offspring produced/day per surviving adult) using the probitmethod via Nuthatch statistical software. The 21-day ECsofor immobility was determined using the moving average method; the probit method did not provide a calculable 95% confidence interval. Parental Survival ECso: 216 mg ai/L NOAEC: 110 mg ai/L LOAEC: 220 mg ai/L Time to First Brood NOAEC: <28 mg ai/L LOAEC: 28 mg ai/L Total Offspring produced ECso: 100 mg ai/L Probit slope: 1.42±0.331 NOAEC: 28 mg ai/L LOAEC: 54 mg ai/L 95% C.I.: 184-252 mg ai/L

95% C.I.: 63-170 mg ai/L

Offspring/dayper surviving adult ECso: 170 mg ai/L 95% C.I.: 81-380 mg ai/L Probit slope: 1.08±0.390 NOAEC: 28 mg ai/L LOAEC: 54 mg ai/L Total Length NOAEC: <28 mg ai/L LOAEC: 28 mg ai/L 

### E. STUDY DEFICIENCIES: This study is considered scientifically sound; however, the source ofthe dilution (fish culture water) could have contained ammoniain the water, which was neithermeasurednor reported in the water quality measurements. After analysis ofthe data the reviewer concludedthat a NOAEC could not be determinedfor this study due to significant (p<0.05) treatment effects on first day ofbroodproduction and adult length at the lowest treatment level.

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### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates

-

### Daphnia sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 F. REVIEWER'S COMMENTS: The reviewer's conclusions differed from the study author's. The study author did not indicate significant effects for time to first brood production and adult length at the lowest level, which the reviewer's analysis detected. Additionally, the reviewer's analysis detected a lower NOAEC for offspring produced/dayper surviving adult than the study author's analysis, as well as for adult immobility. The reviewer's more conservative conclusions regarding time to first brood production and length are presented in the Executive Summary and Conclusions sections. All OPPTS 850.1300 validity requirements were fulfilled. Specifically, control mortality was 10% (no more than 20% permissible), control daphnids each produced an average of96 young (minimum of60 required), and no ephippia were producedby control daphnids. The study authors repeatedly stated in error that the first day ofbrood release, the number of offspring per surviving adult, and length were the three most sensitive endpoints; whereas, the three most sensitive endpoints indicated by the study author's analysis was the day of first brood release, the total number ofoffspring, and length. Test samples collected for analytical verification were stored refrigerated prior to analysis. Aliquots were analyzed for Tribenuron-methyl either directly or following dilution with water using high-performance liquid chromatography (HPLC) with UV detection (230 urn). The experimental phase ofthe definitive study was conducted from July 14 to August 4, 1989.
G. CONCLUSIONS: This study is 

### scientificallysound, however; the study is not acceptable for use in risk assessment. Based upon treatment-related effects on the time to first brood release and terminal lengths ofsurviving daphnia at the 28 mg ai/L treatment level (the most sensitive endpoints), the overall NOAEC for the study could not be determined (i.e., <28 mg ai/L). A treatment-related reduction in offspring production (total and offspring/dayper surviving adult) was also indicated at the 54, 110 and 220 mg ai/L levels, and immobility (i.e., mortality) due to exposure was observed at the 220,400 and 790 mg ai/L levels (with complete immobility at the 400 and 790 mg ai/L levels). The ECso value (with 95% c.I.) 

### for immobility was 216 (184 - 252) mg ai/L and was 100 (63 - 170) mg ai/L for reproduction. Based on treatmentrelatedtreatment-related effects seen in the lowest test concentration, this study is classified as Supplemental (not repairable).

Page 16 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 21-day ECso (mortality/immobility): 216 mg ai/L 95% c.r.: 184-252 mg ai/L (moving average method) 21-day ECso(reproduction): 100 mg ai/L 95% C.r.: 63-170 mg ai/L Slope: 1.42±0.331 NOAEC: <28 mg ai/L LOAEC: 28 mg ai/L Endpoints affected: Survival, time to first brood release, total young produced, young/day per surviving adult, and growth (length) Most sensitive endpoint(s): Time to first brood release and growth (length)


### III. REFERENCES: Hutton, D.G. 1989. "Short-term Study Protocol Sheet", MR-4581-665, Haskell Number 15,527, February 20,1989. "Chronic Toxicity Test on Compounds withDaphnia magna", 

### Haskell laboratory Aquatic Test SOP-T23. OECD. 1984. "OECD Guideline for Testing ofChemicals: 202",4 April 84. Rexrode, M, and T.M. Armitage. 1986. "StandardEvaluation Procedure, Daphnia magna 

### LifeCycle (21-Day Renewal) chronic Toxicity Test". EPA 540/9-86-141, U.S. EPA, Office of Pesticide programs, Washington, DC, July 1986. Hutton, D.G. 1989. "Static Acute 48-HourEC50 ofDPX-L5300-78 to Daphnia magna", 

### Haskell Laboratory Report No. 179-89. May 3, 1989. Miller, 

### R.J. 1981. Simultaneous StatisticalInference, 

### 2nd 

### ed., Springer Verlag, New York. Cochran, W.G. 1954. "Some Methods for Strengthening the Common X2 

### Tests", Biometrics, 

### 10, pp. 417-451. Armitage, P. 1955. "Tests for Linear Trends in Proportions and Frequencies", Biometrics, 11, 

### pp. 375-386. Finney, D.l 1971. Probit Analysis, 3rd

### Ed. Cambridge University Press. 333 pp. Peace, K.E. 1988. BiopharmaceuticalStatisticsfor Drug Development, 

### Marcel Dekker, Inc., New York, NY, pp. 242-243. Page 17 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 Haskell Laboratory MR-5659. United States Geological Society (USGS). 1998. Dissolved Oxygen Saturation Tables (Originators: Radtke, D.B., White, A.F., Davis, J.V., and Wilde, F.D.). Release date: 1998 http://water.usgs.gov/software/DOTABLES/.

Page 18 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp, 

### PMRASubmissionNumber { } EPAMRID No. 

### 47643007 APPENDIX I. 

### OUTPUT OF REVIEWER'S STATISTICAL VERIFICATION: Summary of Fisher's Exact Tests
GROUP IDENTIFICATION NUMBER EXPOSED NUMBER DEAD SIG 0.05

1 2 3 4 5 6 CONTROL 28 54 110 220 400 790 40 40 40 40 40 40 40 4 1 4 8 11 40 40 * 

### * * NOTE: BECAUSE THERE WAS CONTROL MORTALITY, AND NONE OF THE LOWER CONCENTRATIONS PRODUCED ZERO MORTALITY, THE DATA HAS BEEN SUBJECTED TO ABBOTT'S CORRECTION.

************************************************************************

CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (PERCENT) 790 36 36 100 0 400 36 36 100 0 220 36 7.000001 19.4444 0 110 36 4 11.1111 0 54 36 0 0 0 28 40 1 2.5 0 BECAUSE THE NUMBER OF ORGANISMS USED WAS SO LARGE, THE 95 PERCENT CONFIDENCE INTERVALS CALCULATED FROM THE BINOMIAL PROBABILITY ARE UNRELIABLE. USE THE INTERVALS CALCULATED BY THE OTHER TESTS. AN APPROXIMATE LC50 FOR THIS SET OF DATA IS 265.0979 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD

SPAN G LC50 95 PERCENT CONFIDENCE LIMITS 4 3.503224E-02 215.5241 184.1108 252.206
GOODNESS OF FIT PROBABILITY 

### o IS LESS THAN 0.001. THE PROBIT METHOD H 64.42461 MEANS THAT IT RESULTS CALCULATED USING ITERATIONS G 7 .812128 A PROBABILITY OF 0 SINCE THE PROBABILITY IS LESS THAN 0.05, RESULTS CALCULATED USING THE PROBIT METHOD PROBABLY SHOULD NOT BE USED. SLOPE 4.070066 95 PERCENT CONFIDENCE LIMITS =-7.305843 AND 15.44597 INTERCEPT=-9.599594 LC50 = 228.3413 

### Page 19of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY LC25 = 155.9058 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY LC10 = 110.586 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY LC05 = 90.04065 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY *************************************************************************

Title: File: Time to First Brood 3007b Transform:

Shapiro

- Wilk's Test for Normality NO TRANSFORMATION
D = 92.8222 W = 0.9622 Critical W W 0.9290 (alpha 

### = 0.01 , N 

### = 49) 0.9470 (alpha 

### = 0.05 , N 

### = 49) Data PASS normality test (alpha = 0.01). Continue analysis. Title: File: Time to First Brood 3007b Transform: NO TRANSFORMATION

Levene's Test for Homogeneity of variance ANOVA Table SOURCE Between Within (Error) Total DF 4 44 48 SS 2.2961 51.6222 53.9184 MS 0.5740 1.1732 F 0.4893

(p-value = 0.7435) Critical F = 3.7784


### = 2.5837 Since F < Critical F (alpha 

### = 0.01, df 

### = 4,44) (alpha = 0.05, df = 4,44) FAIL TO REJECT Ho: All equal (alpha 0.01) Title: File: Time to First Brood 3007b Transform: ANOVA Table NO TRANSFORMATION
SOURCE Between Within (Error) Total DF 4 44 48 SS 43.1778 92.8222 136.0000 MS 10.7944

2.1096 F 5.1168



### Page 20 of30 (p-value 

### = 0.0018)

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 Critical F 

### = 3.7784 

### = 2.5837 Since F > Critical F (alpha (alpha REJECT 0.01, df 

### = 4,44) 0.05, df = 4,44) Ho: All equal (alpha 0.05) Title: File: Time to First Brood 3007b Bonferroni t-Test Transform: TABLE 1 OF 2 NO TRANSFORMATION Ho: Control>Treatment TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS t STAT 0.05

-------------------- ----------- ------------------ ------ 1 Control 11.4444 11.4444 2 28 13.0000 13 .0000 2.3309 

### * 3 54 13.3000 13.3000 2.7805 

### * 4 110 14.4000 14.4000 4.4288 

### * 5 220 12.7000 12.7000 1.8814 Bonferroni t critical value 

### = 2.3207 (1 Tailed, alpha 

### = 0.05, df 

### = 4,44) Title: File: Time to First Brood 3007b Bonferroni t-Test Transform: TABLE 2 OF 2 NO TRANSFORMATION Ho: Control>Treatment NUM OF MIN SIG DIFF % OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL

-------------------- ------- ---------------- ------- ------------ 1 Control 9 2 28 10 1.5487 13.5 1.5556 3 54 10 1.5487 13.5 1.8556 4 110 10 1.5487 13.5 2.9556 5 220 10 1.5487 13.5 1.2556
Title: File: Time to First Brood 3007b William's Test Transform:

- TABLE 1 OF 2 NO TRANSFORMATION Ho: Control>Treatment ORIGINAL TRANSFORMED ISOTONIZED GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- -------'---- ----------- ----------- 1 Control 9 11.4444 11. 4444 11. 4444 2 28 10 13.0000 13 .0000 13.0000 3 54 10 13.3000 13 .3000 13.3000 4 110 10 14.4000 14.4000 13.5500 5 220 10 12.7000 12.7000 13.5500



### Page 21 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007

Title: File: Time to First Brood 3007b William's Test Transform:

- TABLE 2 OF 2 NO TRANSFORMATION Ho: Control>Treatment COMPARED CALC. SIG TABLE DEGREES OF IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM USED

-------------------- ----------- ----------- ----------- ------------ Control 11.4444 28 13.0000 2.3309 

### * 1.6800 k= 1, v=40 54 13.3000 2.7805 

### * 1.7600 k= 2, v=40 110 13.5500 3.1551 

### * 1.7900 k= 3, v=40 220 13.5500 3.1551 

### * 1.8000 k= 4, v=40

s = 1.4524

WARNING: Procedure has used isotonized means which differ from original (transformed) means. Title: File: Total Offspring 30070 Transform: Chi-Square Test for Normality NO TRANSFORMATION

INTERVAL < -1. 5 Actual and Expected Frequencies -1.5 to <-0.5 -0.5 to 0.5 >0.5 to 1.5 >1. 5 EXPECTED OBSERVED 3.3500 3 12.1000 12 19.1000 19 12.1000 13 3.3500 3 Chi-Square = 0.1414 Critical Chi-Square = 13 .277 9.488 (p-value = 0.9976) (alpha = 0.01 , df = 4) (alpha = 0.05 , df = 4) Data PASS normality test (alpha = 0.01). Continue analysis. Title: File: Total Offspring 30070 Transform: NO TRANSFORMATION

Levene's Test for Homogeneity of Variance ANOVA Table SOURCE Between Within (Error) Total DF 4 45 49 SS 43508.6800 187098.2000 230606.8800 MS 10877.1700

4157.7378 F 2.6161

(p-value = 0.0475) Critical F = 3.7674

= 2.5787 Since F < Critical F (alpha = 0.01, df = 4,45) (alpha = 0.05, df = 4,45) FAIL TO REJECT Ho: All equal (alpha 

### Page 22 of30 0.01)

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp, PMRA Submission Number { } EPA 

### MRID No. 47643007

Title: File: Total Offspring 30070 Transform: ANOVA Table NO TRANSFORMATION
SOURCE Between Within (Error) Total DF 4 45 49 SS 484423.0800 385411.9000 869834.9800 MS 121105.7700 8564.7089 F 14.1401

Critical F = 3.7674
= 2.5787 Since F > Critical F (alpha 

### = 0.01, df 

### = 4,45) (alpha 

### = 0.05, df 

### = 4,45) REJECT Ho: All equal (alpha (p-value 

### = 0.0000) 0.05) Title: File: Total Offspring 30070 Dunnett's Test Transform: TABLE 1 OF 2 NO TRANSFORMATION Ho:Control<Treatment TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.05

-------------------- ----------- ------------------ ------ 1 Control 383.3000 383.3000 2 28 333.4000 333.4000 1.2057 3 54 273.7000 273.7000 2.6481 

### * 4 110 135.1000 135.1000 5.9970 

### * 5 220 149.6000 149.6000 5.6466 

### * Dunnett critical value 

### = 2.2300 (1 Tailed, alpha 

### = 0.05, df [used] 4,40) (Actual df 

### = 4,45) Title: File: Total Offspring 30070 Dunnett's Test Transform: TABLE 2 OF 2 NO TRANSFORMATION HO:Control<Treatment NUM OF MIN SIG DIFF !l- OF DIFFERENCE 0 GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL

-------------------- ------- ---------------- ------- ------------ 1 Control 10 2 28 10 92.2946 24.1 49.9000 3 54 10 92.2946 24.1 109.6000 4 110 10 92.2946 24.1 248.2000 5 220 10 92.2946 24.1 233.7000



### Page 23 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia sp. PMRA Submission Number { } EPA MRID 

### No. 47643007

Title: File: Total Offspring 30070 William's Test Transform:

- TABLE 1 OF 2 NO TRANSFORMATION Ho: Control<Treatment ORIGINAL TRANSFORMED ISOTONIZED GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- ----------- ----------- ----------- 1 Control 10 383.3000 383.3000 383.3000 2 28 10 333.4000 333.4000 333.4000 3 54 10 273.7000 273.7000 273.7000 4 110 10 135.1000 135.1000 142.3500 5 220 10 149.6000 149.6000 142.3500
Title: File: Total Offspring 30070 William's Test Transform: - TABLE 2 OF 2 NO TRANSFORMATION Ho: Control<Treatment COMPARED CALC. SIG TABLE DEGREES OF IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM USED

-------------------- ----------- ----------- ----------- ------------ Control 383.3000 28 333.4000 1.2057 1.6800 k= 1, v=40 54 273.7000 2.6481 

### * 1.7600 k= 2, v=40 110 142.3500 5.8218 

### * 1.7900 k= 3, v=40 220 142.3500 5.8218 

### * 1.8000 k= 4, v=40

s = 92.5457

WARNING: Procedure has used isotonized means which differ from original (transformed) means. Estimates of EC% Parameter Estimate 95% Bounds Std. Err. Lower Bound Lower Upper /Estimate EC5 7.1 1.5 33. 0.33 0.22 EC10 13 . 3.6 46. 0.27 0.28 EC25 34. 15. 80. 0.18 0.43 EC50 1.0E+02 63. 1.7E+02 0.11 0.61 Slope 1. 42 Std.Err. = 0.331 !! lPoor fit: p 0.019 based on DF= 2.0 45.



### Page 24 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 30070: Total Offspring Observed vs. Predicted Treatment Group Means Dose #Reps. Obs. Pred. Obs. Pred. %Change Mean Mean -Pred. %Control 0.00 10.0 383. 393. -9.79 100. 0.00 28.0 10.0 333. 310. 23.6 78.8 21. 2 54.0 10.0 274. 257. 16.7 65.4 34.6 110. 10.0 135. 190. -54.7 48.3 51. 7 220. 10.0 150. 125. 24.1 31. 9 68.1 ! ! !Warning: EC5 not bracketed by doses evaluated. ! ! !Warning: EC10 not bracketed by doses evaluated. Title: File: Young/day per surviving parent 3007y Transform: Chi-Square Test for Normality NO TRANSFORMATION

INTERVAL <-1.5 Actual and Expected Frequencies -1.5 to <-0.5 -0.5 to 0.5 >0.5 to 1.5 >1. 5 EXPECTED OBSERVED 3.3500 3 12.1000 12 19.1000 20 12.1000 12 3.3500 3 Chi-Square 

### = 0.1172 Critical Chi-Square = 13.277 9.488 (p-value 

### = 0.9983) (alpha 

### = 0.01 , df 

### = 4) (alpha 

### = 0.05 , df 

### = 4) Data PASS normality test (alpha 

### = 0.01). Continue analysis. Title: File: Young/day per surviving parent 3007y Transform:

Levene's Test for Homogeneity ANOVA Table NO TRANSFORMATION of Variance
SOURCE Between Within (Error) Total DF 4 45 49 SS 2907.7200 13482.3000 16390.0200 MS 726.9300 299.6067 F 2.4263

(p-value = 0.0616) Critical F = 3.7674 

### = 2.5787 Since F < Critical F (alpha 

### = 0.01, df 

### = 4,45) (alpha 

### = 0.05, df 

### = 4,45) FAIL TO REJECT Ho: All equal (alpha



### Page 25 of30 0.01)

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. PMRA Submission Number { } EPA MRID No. 47643007

Title: File: Young/day per surviving parent 3007y Transform: ANOVA Table NO TRANSFORMATION
SOURCE Between Within (Error) Total DF 4 45 49 SS 20692.6800 28135.8000 48828.4800 MS 5173.1700

625.2400 F 8.2739

Critical F 

### = 3.7674


### = 2.5787 Since F > Critical F (alpha 

### = 0.01, df 

### = 4,45) (alpha = 0.05, df = 4,45) REJECT Ho: All equal (alpha (p-value 

### = 0.0000) 0.05) Title: File: Young/day per surviving parent 3007y Transform: Dunnett's Test TABLE 1 OF 2 NO TRANSFORMATION Ho:Control<Treatment TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.05

-------------------- ----------- ------------------ ------ 1 Control 95.7000 95.7000 2 28 85.2000 85.2000 0.9390 3 54 73.6000 73.6000 1.9763 4 110 40.8000 40.8000 4.9095 * 5 220 52.3000 52.3000 3.8811 * Dunnett critical value = 2.2300 (1 Tailed, alpha 

### = 0.05, df [used] 4,40) (Actual df 

### = 4,45) Title: File: Young/day per survlvlng parent 3007y Transform: Dunnett's Test TABLE 2 OF 2 NO TRANSFORMATION Ho:Control<Treatment NUM OF MIN SIG DIFF % OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL

-------------------- ------- ---------------- ------- ------------ 1 Control 10 2 28 10 24.9369 26.1 10.5000 3 54 10 24.9369 26.1 22.1000 4 110 10 24.9369 26.1 54.9000 5 220 10 24.9369 26.1 43.4000



### Page 26 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007

Title: File: Young/day per surviving parent 3007y Transform: William's Test

- TABLE 1 OF 2 NO TRANSFORMATION Ho: Control<Treatment ORIGINAL TRANSFORMED ISOTONIZED GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- ----------- ----------- ----------- 1 Control 10 95.7000 95.7000 95.7000 2 28 10 85.2000 85.2000 85.2000 3 54 10 73.6000 73.6000 73.6000 4 110 10 40.8000 40.8000 46.5500 5 220 10 52.3000 52.3000 46.5500
Title: File: Young/day per surviving parent 3007y Transform: William's Test - TABLE 2 OF 2 NO TRANSFORMATION Ho: Control<Treatment COMPARED CALC. SIG TABLE DEGREES OF IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM USED

-------------------- ----------- ----------- ----------- ------------ Control 95.7000 28 85.2000 0.9390 1.6800 k= 1, v=40 54 73.6000 1.9763 

### * 1.7600 k= 2, v=40 110 46.5500 4.3953 

### * 1.7900 k= 3, v=40 220 46.5500 4.3953 

### * 1.8000 k= 4, v=40

s = 25.0048

WARNING: Procedure has used isotonized means which differ from original (transformed) means. Estimates of EC%- Parameter Estimate 95% Bounds Std. Err. Lower Bound Lower Upper /Estimate EC5 5.2 0.38 73. 0.57 0.072 EC10 11. 1.4 93. 0.45 0.12 EC25 41. 12. 1.5E+02 0.27 0.28 EC50 1.7E+02 81. 3.8E+02 0.16 0.47 Slope = 1. 08 Std. Err. = 0.390 !! lPoor fit: p = 0.023 based on DF= 2.0 45. 3007Y : Young/day per surviving parent Observed vs. Predicted Treatment Group Means Dose #Reps. Obs. Pred. Obs. Pred. %Change Mean Mean -Pred. %-Control 0.00 10.0 95.7 97.7 -2.02 100. 0.00 28.0 10.0 85.2 78.6 6.56 80.5 19.5 54.0 10.0 73.6 69.3 4.30 70.9 29.1 

### Page 27 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 110. 220. ! ! !Warning: l ! !Warning: 10.0 40.8 57.3 -16.5 10.0 52.3 44.7 7.62 EC5 not bracketed by doses evaluated. EC10 not bracketed by doses evaluated. 58.6 45.7 41.4 54.3

Title: File: Total Length 30071 Transform: Chi-Square Test for Normality NO TRANSFORMATION

INTERVAL <-1. 5 Actual and Expected Frequencies -1.5 to <-0.5 -0.5 to 0.5 >0.5 to 1.5 >1.5 EXPECTED OBSERVED 11.5240 20 41.6240 27 65.7040 72 41.6240 44 11.5240 9 Chi-Square 

### = 12.6638 Critical Chi-Square = 13.277 9.488 (p-value 

### = 0.0130) (alpha = 0.01 , df = 4) (alpha 

### = 0.05 , df 

### = 4) Data PASS normality test (alpha = 0.01). Continue analysis. Title: File: Total Length 30071 Transform: NO TRANSFORMATION

Levene's Test for Homogeneity of Variance ANOVA Table SOURCE Between Within (Error) Total DF 4 167 171 SS 0.2135 3.5565 3.7700 MS 0.0534 0.0213 F 2.5057

(p-value 

### = 0.0441) Critical F = 3.4335


### = 2.4258 Since F < Critical F (alpha 

### = 0.01, df 

### = 4,167) (alpha 

### = 0.05, df 

### = 4,167) FAIL TO REJECT Ho: All equal (alpha 0.01) Title: File: Total Length 30071 Transform: ANOVA Table NO TRANSFORMATION
SOURCE DF SS MS F Between Within (Error) Total 4 167 171 10.1568 7.1949 17.3516 2.5392 0.0431 58.9373

Critical F 3.4335 2.4258 (alpha (alpha 0.01, df 0.05, df 

### Page 28 of30 4,167) 4,167) (p-value = 0.0000)

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to Freshwater Invertebrates -Daphnia 

### sp. PMRA SubmissionNumber { } EPA MRID No. 47643007 Since F > Critical F REJECT Ho: All equal (alpha 

### = 0.05) Title: File: Total Length 30071 Bonferroni t-Test Transform: TABLE 1 OF 2 NO TRANSFORMATION Ho: Control<Treatment TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS t STAT 0.05

-------------------- ----------- ------------------ ------ 1 Control 3.7472 3.7472 2 28 3.6564 3.6564 1.8930 3 54 3.6056 3.6056 2.8957 * 4 110 3.2000 3.2000 10.8513 * 5 220 3.1414 3.1414 11.6977 * Bonferroni t critical value 

### = 2.2618 (1 Tailed, alpha 

### = 0.05, df 

### = 4,167) Title: File: Total Length 30071 Bonferroni t-Test Transform: TABLE 2 OF 2 NO TRANSFORMATION Ho: Control<Treatment NUM OF MIN SIG DIFF % OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL

-------------------- ------- ---------------- ------- ------------ 1 Control 36 2 28 39 0.1085 2.9 0.0908 3 54 36 0.1107 3.0 0.1417 4 110 32 0.1141 3.0 0.5472 5 220 29 0.1171 3.1 0.6058
Title: File: Total Length 30071 William's Test Transform: NO TRANSFORMATION

- TABLE 1 OF 2 Ho: Control<Treatment

'

# .. ORIGINAL TRANSFORMED ISOTONIZED GROUP IDENTIFICATION N MEAN MEAN MEAN

------ -------------------- ----------- ----------- ----------- 1 Control 36 3.7472 3.7472 3.7472 2 28 39 3.6564 3.6564 3.6564 3 54 36 3.6056 3.6056 3.6056 4 110 32 3.2000 3.2000 3.2000 5 220 29 3.1414 3.1414 3.1414



### Page 29 of30

### Data Evaluation Record on the Chronic Toxicity of DPX-L5300-78 (formulation of Tribenuron-methyl) to FreshwaterInvertebrates -Daphnia 

### sp. 

### PMRA SubmissionNumber { } EPA MRID No. 47643007

Title: File: Total Length 30071 William's Test Transform: NO TRANSFORMATION

- TABLE 2 OF 2 Ho: Control<Treatment COMPARED CALC. SIG TABLE DEGREES OF IDENTIFICATION MEANS WILLIAMS 0.05 WILLIAMS FREEDOM USED

-------------------- ----------- ----------- ----------- ------------ Control 3.7472 28 3.6564 1.8930 

### * 1.6600 k= 1, v=120 54 3.6056 2.8957 

### * 1.7300 k= 2, v=120 10 3.2000 10.8513 

### * 1.7500 k= 3, v=120 220 3.1414 11.6977 

### * 1.7700 k= 4, v=120

s = 0.2076



### Page 30 of30