### Appendix I – ECOSAR Runs for DMDS and Methanesulfonic Acid**Figure I-1.** Dimethyl disulfide ECOSAR Run.

SMILES : S(SC)C CHEM : Disulfide, dimethyl CAS Num: ChemID1: ChemID2: ChemID3: MOL FOR: C2 H6 S2 MOL WT : 94.19 Log Kow: 1.87 (KowWin estimate) Melt Pt: Wat Sol: 3000 mg/L (experimental database)

ECOSAR v1.00 Class(es) Found

------------------------------ Neutral Organics Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

=========================== ================== ======== ======

========== Neutral Organics : Fish 96-hr LC50 93.840 Neutral Organics : Fish 14-day LC50 94.690 Neutral Organics : Daphnid 48-hr LC50 51.934 Neutral Organics : Green Algae 96-hr EC50 23.332 Neutral Organics : Fish 30-day ChV 9.694 Neutral Organics : Daphnid ChV 5.901 Neutral Organics : Green Algae ChV 8.729 Neutral Organics : Fish (SW) 96-hr LC50 131.726 Neutral Organics : Mysid Shrimp 96-hr LC50 102.433 Neutral Organics : Fish (SW) ChV 12.708 Neutral Organics : Mysid Shrimp (SW) ChV 9.193 Neutral Organics : Earthworm 14-day LC50 168.789

Note: * = asterisk designates: Chemical may not be soluble enough to measure this predicted effect.

Neutral Organics:

---------------- For Fish LC50 (96-h), Daphnid LC50, Mysid: If the log Kow is greater than 5.0, or if the compound is solid and the LC50 exceeds the water solubility by 10X, no effects at saturation are predicted.

For Fish LC50 (14-day) and Earthworm LC50: If the log Kow is greater than 6.0, or if the compound is solid and the LC50 exceeds the water solubility by 10X, no effects at saturation are predicted.

For Green Algae Acute Toxicity Values: If the log Kow of the chemical is greater than 6.4, or if the compound is solid and the EC50 exceeds the water solubility by 10X, no effects at saturation are predicted for these endpoints.

For All Chronic Toxicity Values: If the log Kow of the chemical is greater than 8.0, or if the compound is solid and the ChV exceeds the water solubility by 10X, no effects at saturation are predicted for these endpoints.ECOSAR v1.00 SAR Limitations:

---------------------------- Maximum LogKow: 5.0 (Fish 96-hr LC50; Daphnid LC50, Mysid LC50) Maximum LogKow: 6.0 (Fish 14-day LC50; Earthworm LC50) Maximum LogKow: 6.4 (Green Algae EC50) Maximum LogKow: 8.0 (ChV) Maximum Mol Wt: 1000

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-**Figure I-2.** Methanesulfonic Acid ECOSAR Run.

SMILES : O=S(=O)(O)C CHEM : Methanesulfonic acid CAS Num: ChemID1: ChemID2: ChemID3: MOL FOR: C1 H4 O3 S1 MOL WT : 96.10 Log Kow: -2.38 (KowWin estimate) Melt Pt: Wat Sol: 1E+006 mg/L (experimental database)

ECOSAR v1.00 Class(es) Found

------------------------------ Neutral Organics-acid Predicted ECOSAR Class Organism Duration End Pt mg/L (ppm)

=========================== ================== ======== ======

========== --> Acid moeity found: Predicted values multiplied by 10

Neutral Organics-acid : Fish 96-hr LC50 5.31e+006 * Neutral Organics-acid : Fish 14-day LC50 5e+006 * Neutral Organics-acid : Daphnid 48-hr LC50 1.57e+006 * Neutral Organics-acid : Green Algae 96-hr EC50 1.11e+005 Neutral Organics-acid : Fish 30-day ChV 4.12e+005 Neutral Organics-acid : Daphnid ChV 90723.766 Neutral Organics-acid : Green Algae ChV 20415.420 Neutral Organics-acid : Fish (SW) 96-hr LC50 1.03e+007 * Neutral Organics-acid : Mysid Shrimp 96-hr LC50 1.21e+008 * Neutral Organics-acid : Fish (SW) ChV 62332.531 Neutral Organics-acid : Mysid Shrimp (SW) ChV 3.82e+007 * Neutral Organics-acid : Earthworm 14-day LC50 4756.764

Note: * = asterisk designates: Chemical may not be soluble enough to measure this predicted effect.

Neutral Organics:

---------------- For Fish LC50 (96-h), Daphnid LC50, Mysid: If the log Kow is greater than 5.0, or if the compound is solid and the LC50 exceeds the water solubility by 10X, no effects at saturation are predicted.

For Fish LC50 (14-day) and Earthworm LC50: If the log Kow is greater than 6.0, or if the compound is solid and the LC50 exceeds the water solubility by 10X, no effects at saturation are predicted.

For Green Algae Acute Toxicity Values: If the log Kow of the chemical isgreater than 6.4, or if the compound is solid and the EC50 exceeds the water solubility by 10X, no effects at saturation are predicted for these endpoints.

For All Chronic Toxicity Values: If the log Kow of the chemical is greater than 8.0, or if the compound is solid and the ChV exceeds the water solubility by 10X, no effects at saturation are predicted for these endpoints.

ECOSAR v1.00 SAR Limitations:

---------------------------- Maximum LogKow: 5.0 (Fish 96-hr LC50; Daphnid LC50, Mysid LC50) Maximum LogKow: 6.0 (Fish 14-day LC50; Earthworm LC50) Maximum LogKow: 6.4 (Green Algae EC50) Maximum LogKow: 8.0 (ChV) Maximum Mol Wt: 1000

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