# Patent

## Title
Pulsed electrochemical detection method

## Abstract

In one embodiment, the present invention is directed to a pulsed electrochemical detection method comprising (a) flowing a liquid sample stream including at least one analyte past a working electrode in a flow-through cell; (b) applying at least three pulsed electrical potentials to the working electrode, (1) a first conditioning potential, (2) a second detection and oxidative cleaning potential, and (3) a third reductive cleaning potential, said second potential being higher than said first and third potentials and said first potential being higher than said third potential; and (c) detecting the output from said working electrode during the time period of applying said second potential.

## Background
BACKGROUND OF THE INVENTION
Direct electrochemical detection of aliphatic compounds with electroactive functional groups based on electrocatalytic reactions at noble metal electrodes, chiefly Au and Pt, is well known. Electrochemical detection is a widely accepted means of detection in liquid and ion chromatography. Electrochemical detectors operate by applying an electrical potential to the working electrode in a flow-through cell. Such detectors typically employ a cell of three electrodes, which are a working electrode, a reference electrode and a counter electrode. Typically, the methodology uses multi-step potential waveforms which incorporate a detection operation along with the anodic and cathodic cleaning of the electrode surface. In a typical potential waveform, anodic detection occurs at a first potential with current sampling at the end of the time period of applying the potential. The potential then is stepped to a second higher potential for oxidative cleaning of the electrode surface, and subsequently to a third potential lower than the first or second potentials for cleaning by cathodic dissolution of the surface oxide formed at the first and/or second potentials. An analytical application of this method using amperometric detection, now known as Pulsed Amperometric Detection (PAD), has been demonstrated for alcohols, polyalcohols and carbohydrates (reducing and non-reducing); amines and amino acids (primary and secondary); aminoglycosides; and numerous sulfur compounds (except sulfate, sulfonic acids and sulfones).
Pulsed Coulometric Detection (PCD) has been described. The significant difference between PAD and PCD lies in the instrumental protocol related to measurement of the detection signal. The term “pulsed electrochemical detection” or “PED” is a general term that encompasses PAD and PCD. In PAD, electrode current is sampled or averaged over a time period (e.g., 16.7 ms) (at 1/60 Hz−1) whereas in PCD the response is electronically integrated over a single period or over an integral number of sequential periods. PCD inherently has a larger signal-to-noise ratio (S/N) because of the larger signal strength and because the integral of a 60-Hz correlated noise signal, a predominant form of noise in electronic instrumentation, remains at zero over the integration period.
Photometric detection suffers because of an inherently low sensitivity for aliphatic compounds without extensive π-bonding and because of baseline drift which accompanies a change in the refractive index of the mobile phase. Refractive index detection is strongly affected by concentration gradients and the baseline shift observed for even small changes in mobile phase composition can overwhelm the analyte signal.
The methods of PAD and PCD were introduced for detection of numerous aliphatic organic compounds with electroactive functional groups in conjunction with liquid chromatography (LC). Numerous PAD/PCD methods have become part of daily routine in academic and industrial laboratories. The majority of such methods utilize gold as a material for the working electrodes. The usage of platinum working electrodes has fallen behind the utilization of gold electrodes. Many analysts have found existing platinum-electrode based methods more difficult to use and less reproducible than those using gold electrodes.
Many analytes are detectable with working electrodes made of different materials. Various electrode materials exhibit different performance in response to sample matrix accompanying the analytes of interest. Example 1: Alcohols are detectable with platinum and gold electrodes. If alcohols are present in samples with high concentrations of sugars, platinum electrodes are preferable because they are less affected by excessive levels of sugars than gold electrodes. Example 2: Cyanide anion can be detected either with a silver electrode or with a platinum electrode. In samples with a high concentration of sulfides, silver electrodes are easily affected and have been observed to lose any response to cyanide after very few injections of high sulfide samples. Platinum electrodes are much more robust in comparison and keep providing detection response to cyanide even after many injections of high sulfide samples. Consequently, there is a need to provide more reproducible electrochemical platinum-based methodology suitable for an expanded list of analytes and which can be performed with high S/N.
SUMMARY OF THE INVENTION
In one embodiment, the present invention is directed to a pulsed electrochemical detection method comprising (a) flowing a liquid sample stream including at least one analyte past a working electrode in a flow-through cell; (b) applying at least three pulsed electrical potentials to said working electrode, (1) a first conditioning and detection potential, (2) a second detection and oxidative cleaning potential, and (3) a third reductive cleaning potential, said second potential being higher than said first and third potentials and said first potential being higher than said third potential; and (c) detecting the output from said working electrode during at least part of the time period of applying said second potential.



BRIEF DESCRIPTION OF THE DRAWING
FIGS. 1A, 1B, and 1C show three (A, B, C) representative potential waveforms useful for the invention.
FIG. 2 illustrates a suitable stepped triple-potential waveform with an integration time period at E2.
FIG. 3A shows a failed attempt to detect acetone and butanone by the prior art method (signal integration at 0.25 V), and FIG. 3B shows an attempt of detecting both ketones using the same waveform but with a signal integration at E2=1.15 V.
FIG. 4 shows a chromatogram exhibiting “oxygen dip” (negative peak 3, lower trace), and the new method (upper trace) minimizing oxygen dip.
FIG. 5 shows the prior art approach and the new method generating a comparable size of response for sulfite anion (45 vs. 41 nC).
FIG. 6 shows the detection of seven different alcohols by the prior art method in the lower trace, and a chromatogram obtained with the improved methodology (Integration: E2).
FIG. 7 shows calibration curves for ethanol by the prior art method (Signal integration at E1)(see waveform in FIG. 1).
FIG. 8 shows data for ethanol obtained by signal integration at the highest potential of the waveform (see E2 in waveform of FIG. 2).
FIGS. 9 and 10 show the linearity improvement for 1-propanol.
FIG. 11 shows a detection of saturated and unsaturated organic acids.
FIG. 12 shows a detection of triple bond in an organic compound.



DETAILED DESCRIPTION OF THE INVENTION
The present invention is directed to pulsed electrochemical detection methods. In one embodiment, the detection is performed by a current integration, known as Pulsed Coulometric Detection (PCD). In another embodiment, the detection is performed amperometrically, commonly known as Pulsed Amperometric Detection (PAD). The present description will refer to these two forms of detection as electrochemical detection unless otherwise specified. An important aspect of the invention is that the output from the working electrodes for detection, e.g., by PCD or PAD, is detected during the time period applying the highest potential of the cycle. In a specific embodiment, at least three different pulsed potentials are applied to the working electrode. The first pulse is a conditioning potential, the second is a detection and oxidative cleaning potential and the third is a reductive cleaning potential. In a preferred embodiment, the second potential is higher than the first and third potentials and the first potential is higher than the third potential. The output from the working electrode is measured during the time period applying the second potential. Thus, the output signal is detected during the highest potential in a pulsed waveform of applying the potential.
By way of illustration, the conditioning potential in FIGS. 1A, 1B, and 1C is illustrated in U.S. Pat. No. 4,939,924 which include waveforms and for purposes of the present invention E1 as the conditioning potential, E2 as the detection and oxidative cleaning potential, and E3 as the reductive cleaning potential. An important difference between the method set forth in the '924 patent and the present invention is that the detection that takes place at the highest potential or E2 in the illustrated figures.
A suitable stepped triple-potential waveform with an integration time period at E2 is illustrated in FIG. 2. All the potentials in the invention are reported against a reference electrode Ag/AgCl/3 M KCl.
The first conditioning potential E1, which is lower than the second detection and oxidative cleaning potential and higher than the third reductive cleaning potential, serves to condition or activate the surface of the working electrodes for detection. In many cases, analytes of interest are undergoing a pre-adsorption at the electrode surface during the electrode conditioning. Typically the electrode surface is metal. Conditioning or activation is accomplished by formation of hydroxide radicals on the metal surface of the electrode or by creation of incomplete monolayers of metal oxides. The hydroxide radicals and freshly created small amounts of oxide are the key elements of catalytic conditions required for oxidative detection of many of organic and inorganic compounds at metal electrodes.
At the second or detection and oxidative cleaning potential, the surface of the electrode typically is still covered by the catalysts (radicals and/or oxides). The catalysts are essentially unstable oxygen compounds making oxygen available in the close proximity to pre-adsorbed analytes. The analytes of interest are being pre-adsorbed either during the conditioning step or during the detection step. Frequently, their pre-adsorption stretches over both steps. As the adsorbed analytes encounter the catalytic sites, oxidative desorption takes place producing electrons which are being detected as a signal. At the potential E2, the rate of the electrode reaction becomes more independent of adsorption rates than at the potential E1. The increased independence of the adsorption rates considerably improves the linearity of detection. Concurrently with the oxidative desorption producing the detection signal, the electrode surface coverage by the oxide typically increases. More stable, less catalytic oxides tend to become predominant, especially if an excessively long time period is selected for the E2 potential. In contrast, the prior art detection (PAD or PCD) takes place at the potential E1 where the rate of oxidative desorption is to a much larger degree controlled by the rate of adsorption and in consequence the linearity of calibration plots is inferior, especially in the case of platinum electrodes.
At the third cleaning reductive potential E3, the working electrode surface is freed of all oxygen species created during the conditioning and detection periods. Simultaneous with the removal of oxides, last remnants of adsorbed analytes and/or their oxidation products, which may have been trapped beneath excessive amounts of non-catalytic oxides, typically are also removed.
The present system can be used for detecting analytes in a liquid sample stream. It is applicable to flow injection analysis (FIA) for detecting one or more analytes in a liquid sample stream. It is particularly useful for detection of analytes in a liquid sample stream which have been previously separated through separation medium such as in a chromatographic column. The separated analytes are detected at the working electrode.
The potential waveform may be a potential step function which is constant during one or more of the three potential pulses or may be in any other form such as a potential sweep function so long as the relative potential levels of the three pulses are as set forth above.
By detection at the highest potential, the level of noise can be minimized and the range of linear calibration can be extended for a number of different analytes. Further, detection at the highest potential would move the signal detection from a range of oxygen reduction to hydrogen peroxide around 0.47 V on the electrodes. Because of that, baseline pulsations and oxygen dip may be eliminated or minimized. Further, the lower potentials of conditioning and cleaning can be optimized without regard to performance during detection. Thus, for example, analyte preadsorption or surface-preconditioning can be optimized at E1 and E3 of the waveform potential prior to detection. Another advantage is that the linearity of calibration is improved for many analytes. Furthermore, some new categories of analytes can be detected, such as ethers and ketones.
Suitable ranges of potentials and times for PED waveforms with platinum electrodes include:














Potential [V] vs. AgAgCl
Time [s]




















E1
−0.10-0.80
0.10-1.0



E2
 0.90-1.80
0.10-1.0



E3
−1.00-0.00
0.10-1.0










Preferred ranges of potentials and times for PED waveforms with platinum electrodes include:














Potential [V] vs. AgAgCl
Time [s]




















E1
0.20-0.50
0.30-0.50



E2
1.10-1.30
0.20-0.40



E3
−0.20-−0.50
0.50-0.70










The detection period may take place during the entire time for applying the second potential, E2 for PED. However, it is preferable to detect only for the last 5%-90%, more preferably the last 10%-30% of the period for apply E2.
The invention has been described with respect to a pulsed electrochemical detection method including at least a first conditioning potential, a second detection and oxidative cleaning potential and a third reductive cleaning potential. It also encompasses systems which include the application of additional potentials before, after, or between the application of these potentials so long as the potential and detection relationship of the above three potentials set forth herein is present.
The system of the present invention is particularly effective for a working electrode in an exterior surface formed of a metal material such as platinum, iridium, palladium, rhodium, ruthenium, and lead. A preferred working material electrode is platinum.
One theoretical explanation of why the detection method of the present invention produces the foregoing advantages, particularly for the foregoing working electrodes is as follows. The choice of a relatively high potential removes the signal generation from the potential range of oxygen reduction to hydrogen peroxide. The artifacts such as “oxygen dip” or baseline pulsation stemming from sudden or periodic fluctuations of oxygen concentration in the eluent typically are thus eliminated. An additional advantage is gained by monitoring the signal producing oxidative desorption at the highest of potentials. The signal producing electrode reaction is no longer controlled by the rate of adsorption to the same degree as at the lower potentials. The range of linear calibration is expanded as a result.
The electrochemical detection method of the present invention is particularly effective for analytes such as inorganic anions, alcohols, sulfur amino acids, thioethers, sulfoxides, phenols, ethers, aldehyes, ketones, alkenes, alkynes, nitriles, amino acids, carboxylic acids and combinations thereof.
Typical potential waveforms for PCD and PAD are shown in FIGS. 1A, 1B, and 1C as illustrated in U.S. Pat. No. 4,939,924 (“the '924 patent”). In PCD or PAD, the initial value of E1 is chosen so that the electrode surface exists in an oxide-poor state (formation of an incomplete monolayer of oxide). Following a delay time of td, the potential is advanced to the value E1′ (or any number of potentials having a value E1′′, E1′′′, E1′′′′ etc. <E2), by a fast potential sweep (FIG. 1B) or a potential step (FIG. 1C) for a time period of ts. The value of E1′ (or E1′′, E1′′′, E1′′′′, etc.) is chosen to cause the formation of surface oxide with the concurrent electrocatalytic oxidative reaction of soluble and/or adsorbed analyte. The potential then is either changed to E2 or is returned to the initial value E1 for the holding period th during which all oxide formed during the potential change to E1 is cathodically stripped from the electrode surface. The total time of the waveform period is the sum of the times of application of E1, E2 and E3. An integrator (analog or digital) is activated during period of application of E1 (or E1′) and remains active throughout period of its application. The output signal from the integrator is sampled at the end of the detection period and the value, or a proportional value, is fed to a recording device. Thereafter, positive and negative pulses can be applied such as E2 and E3 which achieve anodic and cathodic cleaning, respectively. The integrator is reset to zero at the end of the application of E1. The value(s) E1′ is (are) chosen to maximize the extent of oxidative reaction of soluble and absorbed analyte. However, the highest potential should not be so large positive to cause significant anodic solvent breakdown with evolution of O2.
For the PCD waveforms in FIGS. 1B and 1C of the '924 patent, the values of E1 and the time can be sufficiently large to provide complete oxidative cleaning of the electrode and a subsequent step to E2 for further oxidative cleaning may not be needed.
In general, the present invention is applicable to the measurement of irreversible electrochemical processes related to analytes of interest superimposed or coincident with other irreversible or reversible processes. The measurement of surface-oxide catalyzed reactions during the formation of surface oxide is a single example of the PCD and/or PAD technique.
As described herein, the highest potential PCD or PAD method of the present invention is particularly applicable to detection of multiple chemical components in a liquid sample which have been separated by liquid chromatography, particularly high performance liquid chromatography (HPLC). The liquid sample is normally mixed with an eluent including an electrolyte as a developing reagent and passed through a chromatography column. The packing for the column typically would include gel or particulate forms of ion exchange or reverse phase packing. The technology is well developed. For example, see C. F. Simpson, Techniques of Liquid Chromatography, 1983, J. H. Knox, High Performance Liquid Chromatography, 1981, and K. Polkar et al., Liquid Chromatography in Clinical Analysis, 1981.
In addition, the PCD method is useful as a method of detection in flow injection analysis (FIA). Such systems are described in Skeggs, Amer. J. Clin. Path., 28, 311-322 (1957) and in U.S. Pat. Nos. 4,013,413, 4,022,575, 4,177,677, 4,224,033, 4,227,973, 4,314,824, 4,315,754, 4,352,780, 4,399,102, 4,399,225, and 4,504,443. In such systems, these samples are supplied to the detector in a continuous liquid carrier flow rather than by being separated, e.g., in liquid chromatography. This technique is of limited application in comparison to the aforementioned liquid chromatographic system.
The pulsed electrochemical detection method of the present invention applies to the use of a two-electrode cell including a reference electrode and a working electrode and of a three-electrode cell including a reference electrode, a working electrode, and a counter electrode.
EXPERIMENTAL SECTION
Chemicals
Suitable solutions may be prepared from Reagent Grade Chemicals (Fisher Scientific, Fair Lawn, N.J.) and purified water (NANOpure II, Barnlsted Co., Boston Mass.
Instrumentation
Suitable instrumentation for the electrochemical method of the present invention may be of the type set forth in the '924 patent.
The sample volumes injected may be 0.1-200 μl and the flow rate was 0.0001-5.000 ml min−1. The electrochemical flow-through cell may be of the “thin-layer” design (Dionex Corporation, Sunnyvale, Calif.) with a Pt electrode (ca. 0.0078 cm2). A manufacturer supplied Ag/AgCl electrode was used in all experiments.
Procedures
All PED waveforms of FIG. 1 including the potential sweep from E1 to E1′ in waveform of FIG. 1B may be programmed either directly in the Dionex ED50 detector or created in the Chromeleon software and downloaded to the detector during the execution of a chromatographic program.
Limits of Detection and Analytical Calibration
There are two main methods for estimating limits of detection. In the first of the two methods, an average value of baseline noise is determined over a specified period of time and calibrated in concentration units with the help of an injection of known amount of analyte of interest. In the majority of cases, a three-fold multiple of baseline noise expressed in concentration or amount units is specified as a limit of detection. In the second of the two methods, the operator determines a standard deviation in concentration or amount units of a series (n>3) of injections of a single standard of known concentration. The limit of detection is then expressed as a multiple of the calculated standard deviation. The multiplication factor is derived from a selected level of confidence and can be obtained from statistical tables. Analytical calibration is generated from injections of series of standards of different concentrations. The concentration range must be selected to be greater than the expected range of unknown concentrations. From the analytical response to known concentrations of standards, a calibration plot illustrating the dependence of analytical response on concentrations, is constructed. It is well known that linear calibration plots indicate better precision and accuracy than non linear calibration plots.
Effect of Oxygen
Using the conventional approach of signal integration at E1, unequal concentrations of oxygen in the injected samples and in the mobile phase can give rise to a substantial artifact called “oxygen dip.” The oxygen dip is caused by a detection signal due to reduction of dissolved oxygen to hydrogen peroxide at the surface of the working electrode. In the majority of cases, the concentration of oxygen is higher in a sample than in the chromatographic mobile phase. Higher concentration of oxygen results in a greater value of negative current. This is manifested in the chromatograms as a sizeable change of baseline level in the negative direction. Depending on the chromatographic conditions, the resulting baseline disturbance can extend over several minutes. A reproducible detection of the beginning and end of a chromatographic peak depends on low baseline noise and absence of baseline excursions which are not caused by the analytes of interest. If platinum electrodes are used for chromatographic detection, the oxygen dip, if present, is frequently co-eluting with and distorting the shape of important analytes (e.g. methanol and ethanol).
Comparison of Waveforms
The optimization of the waveforms for PCD, e.g., choice of FIG. 1A or 1B vs. FIG. 1C, is influenced by the analyte(s) of interest. For analytes whose detection is inhibited by formation of surface oxide, e.g., alcohols, the waveform depicted in FIG. 1C may not be considered appropriate because the stepwise change of potential from E1 to E2 results in the rapid and extensive formation of oxide with cessation of analytical response. Adsorbed analytes detected by mechanisms which are catalyzed by formation of surface oxide, e.g., sulfur compounds, are expected to be detected well by either waveform. As was the case for many other analytes, ketones and ethers also are detected by processes which are catalyzed by the formation of the surface oxides. The chromatograms in FIGS. 3B and 12 were obtained using the waveform of FIG. 2.
To illustrate the present invention, the following non-limiting examples of its practice are provided.
EXAMPLE 1
New Method Detects a Compound Class which was Undetectable by the Prior Art Methodology
FIG. 3A shows a failed attempt to detect acetone and butanone by the prior art method (signal integration at 0.25 V). Only an unknown impurity, most likely an alcohol (peak 2) generates a detection response. Using the same waveform but with a signal integration at E2=1.15 V (FIG. 3B) both ketones are detected easily.
EXAMPLE 2
Elimination of Oxygen Dip
Using the prior art methodology, many chromatograms exhibit a feature called “oxygen dip” (negative peak 3, lower trace). Referring to FIG. 4, the new method (upper trace) minimizes the oxygen dip by signal integration at a potential which is far removed from the potential range of oxygen reduction to hydrogen peroxide. Even though the peak height of formaldehyde has been reduced by the new method (from 46 to 15 nC), the concurrent lowering of baseline noise (from 0.125 to 0.034 nC) improved the detection limit from 0.82 to 0.66 μM by the new method.
EXAMPLE 3
Minimizing Pulsations by the New Method
Referring to FIG. 5, the prior art approach and the new method generate a comparable size of response for sulfite anion (45 vs. 41 nC). However, the new methodology minimizes the regular baseline pulsations caused by oxygen insertion into the eluent flow by the pump pistons. The corresponding reduction in baseline noise (from 0.5 to 0.03 nC) leads to a marked improvement in the detection limits with the new method (from 0.34 to 0.02 ppm).
EXAMPLE 4
Sensitivity Improvement by Lower Noise
Detection of seven different alcohols by the prior art method is shown in the lower trace of FIG. 6 (Integration: E1). The upper trace of FIG. 6 is a chromatogram obtained with the improved methodology (Integration: E2). The peak heights are actually somewhat lower with the improved method in this particular application example. However, the signal decrease is more than compensated by an improvement in the noise (from 0.3567 nC to 0.0286 nC). The limits of detection calculated as 3× multiples of noise improve by a factor of 4-6 (for example LOD of ethanol 4×).
EXAMPLE 5
Improved Linearity
The plot of FIG. 7 shows calibration curves for ethanol by the prior art method (Signal integration at E1 (see waveform in FIG. 1).
In the plot of FIG. 8, the data for ethanol was obtained by signal integration at the highest potential of the waveform (see E2 in waveform of FIG. 2).
The linearity of calibration is clearly improved by signal integration at the highest potential of a waveform.
The plots of FIGS. 9 and 10 show the linearity improvement for 1-propanol.
EXAMPLE 6
Detection of Compounds with Double Bonds
FIG. 11 shows a detection of saturated and unsaturated organic acids.
Propionic acid has the same number of carbon atoms and a carboxyl as the acrylic acid. The acrylic acid differs only in having a double bond. The higher detection signal in the lower of the two traces is explainable by oxidation of the double bond of acrylic acid at the platinum electrode.
EXAMPLE 7
Detection of Compounds with Triple Bonds
FIG. 12 shows a detection of triple bond in an organic compound.
Butanone and 3-butyn-2-one posses the same number of carbon atom and the same ketonic group. 3-butyn-2-one differs only in the additional triple bond. The higher detection signal in the lower of the two traces is explainable by an oxidation of the triple bond of 3-butyne-2-one at the platinum electrode.
Table 1 provides a list of all compounds and compound classes that are now detectable with platinum electrodes. Two new compound classes have been made accessible to electrochemical detection (ethers and ketones). Sensitivity of detection was improved for all compounds listed in the table.













TABLE 1






Detected &
Detectable

Detectable
New method



Compound
reported
but unreported
Undetectable
by new
improves


class
by prior art
by prior art
by prior art
method
LOD
Comments







Inorganic
x
x(*)

x
x
Sulfite, peroxide,


anions





azide*, cyanide*,








iodide, thiocyanate,








iodate, arsenite,








bromide, molybdate


sulfur AA
x


x
x
Only cysteine








reported


thioethers

x

x
x


sulfoxides

x

x
x


alcohols
x


x
x
also carbohydrates


phenols
x


x
x
also catecholamines


ethers


x(**)
x

**some ethers are








detectable, i.e.








methoxy


aldehydes
x


x
x


ketones


x
x


alkenes

x

x


alkynes

x

x


nitriles

x

x


carboxylic
x


x
x


acids





*indicates inorganic anions which are detectable by the prior art methods but remained unreported. Their detection on platinum electrodes is described here for the first time.


**qualifies the statement about ethers being undetectable by the prior art method. A subset of ether group, methoxy compounds, can be detected by prior art.

## Claims

1. A pulsed electrochemical detection method comprising the steps of
(a) flowing a liquid sample stream including at least one analyte past a working electrode in a flow-through cell;
(b) applying at least three pulsed electrical potentials to said working electrode, (1) a first conditioning potential, (2) a second detection and oxidative cleaning potential, and (3) a third reductive cleaning potential, said second potential being higher than said first and third potentials and said first potential being higher than said third potential; and
(c) detecting the output from said working electrode during at least part of the time period of applying said second potential.

2. The method of claim 1 in which said working electrode has an exterior surface formed of a material selected from the group consisting of platinum, iridium, palladium, rhodium, ruthenium, and lead.

3. The method of claim 2 in which said working material exterior surface is platinum.

4. The method of claim 1 in which said output is not detected during the time period of applying said first or third potential.

5. The method of claim 1 in which said flow-through cell is a two-electrode cell including a reference electrode and a working electrode.

6. The method of claim 1 in which said flow-through cell is a three-electrode cell and one of said three electrodes is a reference electrode.

7. The method of claim 1 in which said liquid sample stream includes at least one analyte, said method further comprising flowing said liquid sample stream through separation medium to separate said analytes to form separated analytes in said liquid sample, and detecting said separated analytes.

8. The method of claim 7 in which said separation is performed by liquid chromatography.

9. The method of claim 1 in which said analyte includes one or more compounds selected from the group consisting of inorganic anions, alcohols, sulfur amino acids, thioethers, sulfoxides, phenols, ethers, aldehyes, ketones, alkenes, alkynes, nitriles, amino acids and carboxylic acids.

10. The method of claim 1 in which said detecting is performed by current integration.

11. The method of claim 1 in which said detecting is performed amperometrically.

12. The method of claim 1 in which the external surface of the working electrode includes a surface oxide during application of the second potential and is in an oxide free state during application of the third potential.

13. The method of claim 1 in which said first potential is applied at least in part in a potential sweep function.

14. The method of claim 1 in which said first potential is applied at least in part in a potential step function.