# Patent

## Title
6-(2, 3, 4, 5-tetrahydro-1H-benzo [D] azepin-7-yloxy) -nicotamide derivatives as radio labelled ligands

## Abstract

The present invention relates to radiolabelled compounds of formula (I), which are useful for the labelling and diagnostic imaging of histamine H3 receptor functionality.

## Background
This application is filed pursuant to 35 U.S.C. § 371 as a United States National Phase Application of International Patent Application Serial No. PCT/EP2006/000112 filed Jan. 5, 2006, which claims priority from Great Britain Application No. 0500283.7 filed in the United Kingdom on Jan. 7, 2005, and Great Britain Application No. 0500592.1 filed Jan. 12, 2005, the contents of which are incorporated herein by reference.
The present invention relates to radiolabelled ligands for the histamine H3 receptor, useful for the labelling and diagnostic imaging of the histamine H3 receptor functionality.
It is well established that histamine acts as a neurotransmitter via three distinct histamine receptor subtypes, H1, H2 and H3. The histamine H3 receptor has been reported to play a role as a regulating receptor-system controlling not only the release and synthesis of histamine1 but also the release of other neurotransmitters, such as acetylcholine,2 noradrenaline,3 dopaminergic4 and serotonergic5 systems.
Noninvasive, nuclear imaging techniques can be used to obtain basic and diagnostic information about the physiology and biochemistry of living subjects, including experimental animals, patients and volunteers. These techniques rely on the use of imaging instruments that can detect radiation emitted from radiotracers administered to living subjects. The information obtained can be reconstructed to provide planar and tomographic images which reveal the distribution and/or concentration of the radiotracer as a function of time.
Positron emission tomography (PET) is a noninvasive imaging technique that offers the highest spatial and temporal resolution of all nuclear medicine imaging modalities and has the added advantage that it can allow for true quantitation of tracer concentrations in tissues. The technique involves the use of radiotracers, labelled with positron emitting radionuclides, that are designed to have in vivo properties which permit measurement of parameters regarding the physiology or biochemistry of a variety of processes in living tissue.
Compounds can be labelled with positron or gamma emitting radionuclides. The most commonly used positron emitting radionuclides are 15O, 13N, 11C and 18F, which are accelerator produced and have half lifes of 2, 10, 20 and 110 minutes respectively. The most widely used gamma emitting radionuclides are 18F, 99mTc, 201TI and 123I.
H3 receptor ligands have been described such as thioperamide,6 FUB372,7 ciproxifan8 and radioligands such as [125I]iodoproxifan9 developed for in vitro binding studies. PET and SPECT radiolabelled compounds have also been reported e.g. [123I]iodoproxifan,10 [123I]GR190028,10 [123I]FUB271,10 [18F]VUF5000,11 [11C]UCL182912 and [18F]FUB272.12 
However, none of these radiolabelled compounds have been shown to allow in vivo imaging of histamine H3 receptors in the brain due to low brain uptake (e.g. [123I]GR190028, [123I]FUB271, [18F]FUB, [11C]UCL, [18F]VUF5000), homogenous brain distribution (e.g. [18F]FUB, [11C]UCL) and/or no significant alteration of the brain distribution by selective H3 receptor ligand (e.g. [123I]iodoproxifan, [18F]VUF5000).
WO2004/056369A113 discloses a series of benzodiazepine derivatives said to be histamine H3 receptor antagonists and claimed to be useful in the treatment of various neurological disorders. When Example 121 of WO2004/056369A1 is radiolabelled it has been found to allow in vivo imaging of H3 receptors in the brain.
Accordingly the present invention provides a compound of formula (I):




for use as an histamine H3 ligand, wherein:

R1 is a radiolabelled group incorporating or consisting of a radionuclide selected from 3H, 11C, 13N, 15O, 76Br, 18F, 123I, 125I, 131I, 75Br, 76Br, 77Br, 82Br and X is C;
or R1 is C2-6alkyl and X is 11C;
or a pharmaceutically acceptable salt thereof.
In one embodiment a compound of formula (I) is [11C-N-methyl]-6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-nicotamide (Compound A).
The present invention also provides a radiopharmaceutical composition which comprises a compound of formula (I) or [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide and a pharmaceutically acceptable carrier or excipient.
The present invention also provides the use of a compound of formula (I) or [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide for the manufacture of a medicament for the treatment or prophylaxis of neurological diseases including Alzheimer's disease, dementia, age-related memory dysfunction, mild cognitive impairment, cognitive deficit, epilepsy, neuropathic pain, inflammatory pain, migraine, Parkinson's disease, multiple sclerosis, stroke and sleep disorders including narcolepsy; psychiatric disorders including schizophrenia (particularly cognitive deficit of schizophrenia), attention deficit hypereactivity disorder, depression and addiction; and other diseases including obesity, asthma, allergic rhinitis, nasal congestion, chronic obstructive pulmonary disease and gastrointestinal disorders.
The present invention further provides a method for labelling histamine H3 receptors in a mammal which comprises administering to a mammal an effective amount of a compound of formula (I) or [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide.
The present invention also provides a method for diagnostic imaging of histamine H3 receptors in a mammal which comprises administering to a mammal an effective amount of a compound of formula (I) or [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide.
The present invention also provides a method for diagnostic imaging of tissues expressing histamine H3 receptors in a mammal which comprises administering to a mammal an effective amount of a compound of formula (I) or [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide.
The present invention also provides a method for diagnostic imaging of histamine H3 receptors in the brain of a mammal which comprises administering to a mammal an effective amount of a compound of formula (I) or [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide.
The present invention further provides a method for the detection or quantification of histamine H3 receptor functionality in mammalian tissue which comprises administering to a mammal in which such detection or quantification is desired an effective amount of a compound of formula (I) or [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide.
Preferably, in the methods of the present invention the mammal is human.
The present invention also relates to a process for the preparation of [11C-N-methyl]-6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-nicotamide which comprises reacting 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-nicotamide with [11C]methyl iodide.
Suitable radionuclides that may be incorporated in compounds of formula (I) include: 3H, 13N, 15O, 76Br, 18F, 123I, 125I, 131I, 75Br, 76Br, 77Br, 82Br and 211At. The choice of radionuclide to be incorporated into compounds of formula (I) will depend on the specific analytical or pharmaceutical application. Therefore, for in vitro labelling of histamine H3 receptors and for competition assays compounds that incorporate 3H, 125I or 77Br would be preferred. For diagnostic and investigative imaging agents, compounds that incorporate a radionuclide selected from 11C, 18F, 123I or 76Br are preferred. Incorporation of a chelating radionuclide may be useful in certain applications.
Radiolabelled analogues of compound (I) may be used in clinical studies to evaluate the role of histamine H3 receptor ligands in a variety of disease areas where histamine H3 receptor ligands are believed to be involved.
Scheme 1 represents a synthetic route towards compounds of formula (I) wherein R1 is a radiolabelled group and R1 is a leaving group.




One synthetic route for the synthesis of a compound of formula (II) is shown in Scheme 2.




The starting materials and other reagents are available commercially or can be synthesised by well-known and conventional methods.


EXAMPLE 1
[11C-N-methyl]-6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-nicotamide (Compound A)




Compound A was prepared by N-alkylation of the carboxamide precursor 1B using cyclotron-produced [11C]methyl iodide. Carbon-11 was produced as [11C]CO2 by bombarding nitrogen with 16.5 MeV protons according to the 14N(p,α)11C reaction, the presence of a small amount of oxygen (0.5%) in the target gas converting the 11C into [11C]CO2. Subsequently, [11C]CO2 was converted into [11C]MeI by catalytic reduction (Ni) which give the [11C]CH4 intermediate followed by gas phase iodination with iodine. The [11C]methyl iodide was delivered to the reaction containing the precursor 1B and the tetrabutylammonium fluoride in dimethysulfoxide at room temperature. The reaction mix was heated at 130° C. for 5 min. Following a 70 min irradiation, typical syntheses provide 1.8 to 2.6 GBq of Compound A. For all the productions, the radiochemical purity was greater than 99% and the specific activity ranged from 260 to 1300 GBq/umol. The average total synthesis time including HPLC purification and formulation was approximately 40 min from the end of bombardment.
The precursor (1.0 mg) dissolved in dimethylsulfoxide (300 uL) was placed in a 1 mL glass vial. 20 uL of tetrabutylammonium fluoride were added. The [11C]CH3I was delivered as a gas to the reaction vial and bubbled through the solution containing the precursor at room temperature. After delivery of [11C]CH3I, the sealed vessel was heated at 130° C. for 5 min and injected onto the semi-prep HPLC column (Sphereclone ODS(2) C-18 250×10 mm). HPLC purification was performed at a 10 mL/min flow rate with a mobile phase consisting of acetonitrile and a solution of ammonium formate (50 mM) (42:58). The product fraction collected after approximately 7.7 min was evaporated to dryness and reformulated in 10 ml of 0.9% NaCl. Quality controls were performed on a Sphereclone ODS(2) C-18 250×4.6 mm using acetonitrile and a solution of ammonium formate (50 mM) (48:52) as mobile phase at a flow rate of 3 ml/min.
The present specification also describes the process for the preparation of [11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide (Compound B) by reacting 3-cyclobutyl-7-[(5-iodo-2-pyridinyl)oxy]-2,3,4,5-tetrahydro-1H-3-benzazepine with [11C]BH3.CO complex in presence of a palladium(0) catalyst (WO2005/014479).
EXAMPLE 2
[11C-carbonyl] 6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-N-methylnicotamide  (Compound B)




[11C]Carbon dioxide was produced by the 14N(p,α)11C nuclear reaction using a nitrogen gas target (containing 1% oxygen) pressurised to 150 psi and bombarded with 16.5 MeV protons using the General Electric Medical Systems PETtrace 200 cyclotron. Typically, the irradiation time was 30 minutes using a 40 μA beam current. After irradiation, [11C]carbon dioxide was trapped and concentrated on 4 Å molecular sieves. The trapped [11C]CO2 was released from molecular sieves in a stream of nitrogen (30 mL/min) by heating them to 350° C. [11C]CO2 was reduced on-line to [11C]carbon monoxide after passing through a quartz tube filled with zinc granular heated to 400° C. The produced [11C]CO was condensed onto a trap at −196° C. made from a 12-inch coil of 1/16′′ stainless steel tubing, 0.040′′ i.d., packed with carbonex 1000, 45/60 mesh (Supelco). After 6 min delivery and trapping of the [11C]CO, the radioactive gas was then released at room temperature and carried out through an empty vial in a flow of nitrogen (6 mL/min) into a reactor loaded with the BH3.THF solution (1.5 mL of a 1.0 M solution in THF) in order to form the [11C]BH3CO complex. The complex was then carried with the flow of nitrogen through an empty vial cooled at −78° C., and finally through the reaction vial cooled at −78° C. The reaction vial was prepared as follow: palladium(II) diacetate (0.5 mg, 0.0022 mmol) and triphenylphosphine (2.9 mg, 0.011 mmol) were dissolved in 400 μL of a solution of THF with 1% H2O (degassed by bubbling N2 through it for few minutes). Then, a mixture of 3-cyclobutyl-7-[(5-iodo-2-pyridinyl)oxy]-2,3,4,5-tetrahydro-1H-3-benzazepine (1.6 mg, 0.00365 mmol), DIPEA (1.53 μL, 0.0088 mmol) and methylamine 2.0 M (0.011 mmol, 5.48 μL solution in THF) were dissolved in 300 μL of THF with 1% H2O (degassed by bubbling N2 through it for 5 minutes) and added to the solution of the palladium complex. After trapping of the [11C]BH3.CO, the reaction mixture was heated at 140° C. for 8 min, filtered and analysed for radioactivity content. The analysis of the HPLC chromatograms showed the formation of the desired [11C-carbonyl] 6-[(3-cyclobutyl-2,3,4,5-tetrahydro-1H-3-benzo[d]azepin-7-yl)oxy]-N-methylnicotinamide in approximately 44% yield.
Biological Data
1. In vitro Activity
The in vitro affinity of unlabelled Compound A for the histamine H3 receptor was determined by competition assay using [125I]iodoproxifan as radioligand binding to cell lines stably expressing human histamine H3 receptor.13 
Unlabelled Compound A showed a very high affinity for the human histamine H3 receptor with a pKi value of 9.59 and a high selective over other receptors.







TABLE 1







Binding affinity (pKi) of unlabelled compound A











Human receptor
H3
H1
H2
H4





pKi
9.59
5.6
<5.5
<5.5









2. In vivo Imaging

2.1 PET Imaging with Compound A

The animals (pig, Yorkshire/Danish Landrace ( ̃40 Kg; n=2) were housed singly in thermostatically controlled (20° C.) and naturally illuminated stalls. They were scanned under terminal anaesthesia (ketamine induced isoflurane anaesthesia) on different days. The left femoral artery and vein of each animal were surgically cannulated using catheters (Avanti® size 4F-7F). Blood samples were collected from the femoral artery and the radiolabelled and non-labelled agents were injected to the femoral vein. Animals were placed supine in a Siemens ECAT EXACT HR tomograph, with the head immobilized in a custom-made holding device. During the study, blood pH, pCO2 and pO2 levels were monitored and maintained within the normal physiological range. In addition, BP and heart rate were recorded throughout the study. Compound A was administered intravenously in the femoral vein as a 1 minute bolus injection. PET scanning and arterial blood sampling was commenced upon start of the radioligand administration.
PET images were acquired from 0 to 90 min following administration of Compound A. Compound A readily enters the pig brain; the radioactivity reached its peak uptake at 25 min after administration of the radiotracer and then steadily declined over the remainder of the study. The regional brain distribution of Compound A reflected the known distribution of the histamine H3 receptor with a higher accumulation in the striatum, cortices, thalamus and hypothalamus. Compound A concentration was low in cerebellum, a brain region known to possess very low level of H3 receptors. The highest ratios relative to cerebellum occurred at the end of the study (85 min) and were 9.0, 5.3, 4.9, 3.8 and 3.4 for the striatum, frontal cortex, parietal cortex, occipital cortex and thalamus respectively.
2.2 PET Imaging with Pharmacological Challenges
Four sequential high specific activity iv radioligand Compound A administrations were performed in same animal, same day. Following a baseline scan, Compound A was co-administered with escalating dose of unlabelled unlabelled compound A (0.005, 0.05 and 0.5 mg/kg). [15O]CO and [15O]H2O were administered pre and post administration of unlabelled compound A to monitor and to correct for changes in cerebral blood volume and changes in cerebral blood flow. Following administration of unlabelled compound A, the specific uptake of radiotracer was blocked leading to a homogenous distribution of radioactivity throughout the brain. A similar experiment was performed using escalating dose of ciproxifan (0.006, 0.06, 0.6 and 2.0 mg/kg), a known selective histamine H3 antagonist. A dose-dependent decrease of compound A uptake in the H3-rich regions of the brain was observed. When 2.0 mg/kg of ciproxifan was administered, the ratios tissue to cerebellum reached 1.22, 1.08, 0.99, 0.91 and 0.72 for the striatum, frontal cortex, occipital cortex, parietal cortex and thalamus respectively at 85 min post-injection.

## Claims

1. [11C-N-methyl]6-(3-cyclobutyl-2,3,4,5-tetrahydro-1H-benzo[d]azepin-7-yloxy)-nicotamide.

2. A pharmaceutical composition which comprises a compound according to claim 1.

3. A method for labelling histamine H3 receptors in a mammal which comprises administering to a mammal an effective amount of a compound according to claim 1.

4. A method according to claim 3 which additionally comprises detection of the labelled histamine H3 receptors by positron emission tomography.

5. A method for diagnostic imaging of histamine H3 receptors which comprises administering to a mammal an effective amount of a compound according to claim 1.