CONTROLLED//PROPIN//BASIC 

CVB-DAT-0148.03 Page 1 of 2 

Controlled by: APHIS Center for Veterinary Biologics, 515-337-6100 

United States Department of Agriculture

Animal and Plant Health Inspection Service

Center for Veterinary Biologics

P. O. Box 844

Ames, IA 50010

1. Reagent Name: Secondary Reference Serum-Equine IgG; Stock for Equine IgG Species 

Standard 

2. Strain or Source: Defibrinated Equine plasma 

3. Lot Number: 10849 

4. Fill Date: March 2003 

5. Expiration Date: April 30, 2025 

Precautions: This material has Sodium Azide at a final concentration of 0.1% w/v. Skin 

exposure should be treated by washing with soap and water. Eye exposure should be 

treated by flushing with a running stream of water under low pressure for at least twenty 

minutes. Cases of ingestion or injection need immediate consultation with a health care 

professional. Disposal should be conducted in consultation with the institutional safety 

committee.

6. Intended Use: This serum based secondary reference is for use in assays designed to 

detect and quantify equine IgG. Detection methods are limited to those relying on specific 

reagents such as antibodies directed against equine IgG since other equine serum proteins 

including immunoglobulins are present in this preparation. This is a concentrate and must be 

diluted as indicated in “Instructions for Use” for preparation of the Equine IgG Species Standard. 

7. Instructions for Use: This preparation is provided as frozen preparation containing 

28.02 mg/mL ± 0.56 mg/mL (2SD) (2802 mg/dL or 2802 mg%) Equine IgG, that is diluted 1/6 

(1 part Secondary reference equine serum IgG is diluted with 5 parts of buffer). This final 

material is the equine IgG Species Standard described in 9 CFR 113.499(a).6. The value is 

nominally at 400 mg/dL which is accepted as the serum level of IgG consistent with passive 

protection in foals and is within the margin of error for the assay. The recommended buffer is 

0.05M Sodium Phosphate containing 0.15M NaCl at pH 7.2. 

8. Test of Reagent: This reagent is positive for equine IgG and contains 28.02 mg/mL 

(2802 mg/dL or 2802 mg%). 

9. Container Size, Type, Weight, or Volume: 2.0-mL cryogenic vials; 1.0 mL/vial. 
CONTROLLED//PROPIN//BASIC 

CVB-DAT-0148.03 Page 2 of 2 

Controlled by: APHIS Center for Veterinary Biologics, 515-337-6100 

10. Storage Conditions: It is recommended that the contents of each vial be dispensed in 

aliquots of approximately 200 μL upon receipt and then frozen at -20°C or less in a sealed 

container. This precaution is essential in order to avoid the denaturing effects of repeated 

freezing and thawing and to conserve the reference. 

11. CVB Technical Contact: Bacteriology Section, Center for Veterinary Biologics, (515) 

337-6100. 

12. Origin and Passage History: N/A

13. Method of Preparation: Pooled equine plasma from healthy animals was defibrinated, 

delipidated, dialyzed, concentrated, and filtered. Buffer pH 7.4 contains Tris, NaCl, EDTA, 

PMSF. 

14. Other: None. 

Reagent orders and feedback should be sent including phone number to the following email 

address: VS.DB.CVB.Reagent.Requests@usda.gov

Reagent orders forms (APHIS Form 2018) can be found on the CVB website.