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APHIS-Approved Packing House Procedures for 

Guignardia citricarpa,

Causal Agent of Citrus Black Spot (CBS)

Treatment of fruit with fungicides, chemicals, and disinfectants alone, is not a substitute for 

all the additional requirements of the CBS Federal Order. Packing houses and processors 

must have a signed compliance agreement with APHIS.

All fungicides, chemicals, and disinfectants that are used must be registered for use in the State 

in which they are being applied. The treatment must be applied in accordance with all label 

directions. Applications must be made within the confines of a controlled production facility or 

packing house that has a signed compliance agreement with APHIS. Precautions must be taken 

to prevent contamination of water, and contact with all wildlife, including federally protected 

species.

A. Regulated fruit of Citrus spp. from a CBS quarantined area can move interstate with a 

Certificate to all States provided it has met all conditions of the CBS Federal Order and is 

treated as follows:

1. Washed, and

2. Brushed, and

3. Surface disinfested with at least one of the following treatments, and

Treatment: D301.75-11(a-1) — Chemical Treatment

Thoroughly wet the fruit for at least 2 minutes with a solution containing 200 parts 

per million sodium hypochlorite. Maintain the solution at a pH of 6.0 to 7.5.

Treatment must be applied in accordance with label directions.

Treatment: D301.75-11(a-2) — Chemical Treatment

Thoroughly wet the fruit with a solution containing sodium o-phenyl phenate at a

concentration of 1.86 to 2.0 percent of the total solution. If the solution has sufficient 

soap or detergent to cause a visible foaming action, wet for 45 seconds. If the solution 

does not contain sufficient soap to cause a visible foaming action, wet for 1 minute.

Treatment must be applied in accordance with label directions.

Treatment: D301.75-11(a-3) — Chemical Treatment

Thoroughly wet the fruit with a solution of 85 parts per million peroxyacetic acid 

(PAA) for at least 1 minute. Treatment must be applied in accordance with label 

directions.

4. Treated with label rates of imazalil and/or thiabendazole at the time of packing, and

5. Waxed
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B. Regulated fruit of Citrus spp. from a CBS quarantined area, can move 

interstate with a Limited Permit to non-commercial citrus producing States 

provided it meets all other conditions of the CBS Federal Order, and is 

treated as follows:

1. Washed, and

2. Brushed, and

3. Surface disinfested with a treatment listed in A3 above or an organic 

disinfectant, such as PAA or hydrogen dioxide (also known as hydrogen 

peroxide). Treatments must be applied in accordance with label directions.

C. References

USDA APHIS PPQ. Treatment Manual. Sections D301.75-11 (a-1), (a-2), and (a-3). 

https://www.aphis.usda.gov/plant-health/online-manuals

USDA APHIS PPQ, December 2010. Risk assessment of Citrus spp. fruit as a 

pathway for the introduction of Guignardia citricarpa Kiely, the organism that causes 

Citrus Black Spot disease Center for Plant Health Science and Technology, Plant 

Epidemiology and Risk Analysis Laboratory, Raleigh, NC, USA.

USDA APHIS PPQ, January 2011. Federal Domestic Quarantine Order. Guignardia 

citricarpa Kiely, Causal Agent of Citrus Black Spot (CBS). 

https://www.aphis.usda.gov/planthealth/blackspot

USDA APHIS PPQ, March 2011. Federal Domestic Quarantine Order. Guignardia 

citricarpa Kiely, Causal Agent of Citrus Black Spot (CBS). 

https://www.aphis.usda.gov/planthealth/blackspot

USDA APHIS PPQ, May 2011. Federal Domestic Quarantine Order. Guignardia 

citricarpa Kiely, Causal Agent of Citrus Black Spot (CBS). 

https://www.aphis.usda.gov/planthealth/blackspot

USDA APHIS PPQ, December 2011. Federal Domestic Quarantine Order. 

Guignardia citricarpa Kiely, Causal Agent of Citrus Black Spot (CBS). 

https://www.aphis.usda.gov/planthealth/blackspot

USDA APHIS PPQ, March 2012. Federal Domestic Quarantine Order. Guignardia 

citricarpa Kiely, Causal Agent of Citrus Black Spot (CBS). 

https://www.aphis.usda.gov/planthealth/blackspot