Health Certificate No ...................... 

HEALTH CERTIFICATE FOR EXPORT OF BOVID SEMEN[Specifically Bovine (Bos taurus, Bos indicus, Bison 

bison), Water buffalo (Bubalus bubalis), Yak (Bos grunniens)] FROM FOOT-AND-MOUTH DISEASE-FREE 

MEMBER STATES OF THE EUROPEAN UNION TO THE UNITED STATES OF AMERICA 

1. EU Member State of provenance and competent 

authority: 

2. Health certificate No. 

A. ORIGIN OF SEMEN 

3. Approval number of the semen collection center ______________________________________________________ 

4. Name and address of thesemen collection center: 5. Name and address of the consignor: 

4a. Name and address of the semen sexing facility, if applicable: 

6. Country and place of loading: 7. Means of transport: 

B. DESTINATION OF SEMEN 

8. Name and address of the consignee: 

C. IDENTIFICATION OF SEMEN 

9.1 Name of 

donor bull 

9.2 

Breed 

9.3 

Age 

9.4 

Identification 

Number 

9.5 

Numb 

er of 

straws 

9.6 Date 

of 

collecti 

on 

9.7 

Collection 

code 

9.8 Indicate one: 

sexed semen or 

non- sexed 

semen 

10. Seal number of container(s): 

_________________________________________________________________________________ 

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Health Certificate No ...................... 

D. HEALTH INFORMATION 

Section A (to be signed by the Center Veterinarian) 

11. I, the undersignedCenter Veterinarian of thedescribed semen collection center, hereinafter “SCC,” certify 

that: 

11.1. 

11.1.1 

11.1 .2 . 

11.1 .3 . 

11.1 .4 . 

11.1 .5 . 

All bovid animals in the above SCC were: 

Established as residents only if admitted by a formal process of quarantine, observation, and testing as 

required by legislation in force, notably Annex B to Council Directive 88/407/EEC, as amended by Directive 

2003/43/EC or updated in Regulation (EU) 2016/429; 

Admitted to the SCC herd only after having been proven free of brucellosis, tuberculosis, bovine genital 

campylobacteriosis and trichomoniasis; 

Admitted to the SCC herd only after having been proven free of viremia from persistent bovine vira l 

diarrhea virus infection before entry into the SCC resident herd; and 

Were tested annually for brucellosis, tuberculosis, bovine genital campylobacteriosis, and 

trichomoniasis. 

The semen for export to the United States was either (SELECT ONE): 

 Collected prior to June 1, 2011; OR 

 The semen in the consignment was collected after June 1, 2011, from donors that were negative to two 

serum neutralization tests (using a 1:8cutoff titer) for Schmallenberg virus, with the first performed 

within 30 days prior to collection, and the second between 28 and 60 days after collection. Tests were 

performed in a laboratory approved by the national Competent Authority. 

11.2 . 

11.2 .1 . 

11.2 .2 . 

11.2 .3 . 

11.2 .4 . 

11.2 .5 . 

11.2 .6 . 

In the SCC: 

The SCC is certified as clinically free of para tuberculosis. 

The herd was tested for brucellosis, tuberculosis, bovine genital campylobacteriosis, and trichomoniasis in its 

entirety with negative results at the most recent herd test prior to the period of semen collection for export to 

the United States of America (USA); 

No clinical or other evidence of brucellosis, tuberculosis, bovine genital campylobacteriosis, trichomoniasis 

or leptospirosis was found since the most recent herd test and prior to the embarkation of semen to the United 

States; 

There was no evidenceto indicate that thedonors have been affected with tuberculosis or brucellosis during 

the 12 months prior to the collection of semen for export to the United States; 

There was no clinical evidence of infection by bovine viral diarrhea virus, bluetongue virus, enzootic 

hemorrhagic disease (EHD) or infectious bovine rhinotracheitis virus during the 60 days prior to and during 

the period of collection of semen for export to the United States; and 

All bulls passed a testing program with negative results consistent with the World Organization for Animal 

Hea lth (WOAH, formerly OIE) Terrestrial Animal Health Code (Article 4.5.5) or as outlined in Council 

Directive 88/407/EEC, as amended, a nd updated in Regulation (EU) 2016/429 or updated in Regulation (EU) 

2016/429 to detect persistent testicular bovine viral diarrhea virus infection prior to semen release. 

11.3 . 

11.3 .1 . 

11.3 .2 . 

11.3 .3 . 

11.3 .4 . 

11.3 .5 . 

Each donor bull for the semen described above: 

Originated from a tuberculosis-free herd; 

Was not corralled, pastured, or held with animals of lesser health status or under any restrictions which would 

makethem ineligible to export semen to theUnited States during the 60 days prior to and during the period of 

collection of semen for export to the United States; 

Was subjected with negative results to thetestdescribed in 11.4.1 to 11.4.4 within six months prior to or six 

months after collection of the semen described above; 

Was subjected with negative results to the tests for bluetongue virus group (BTV) described in11.4.5; 

Was inspected on the date of semen collection and found to be free of clinica l signs of diseases 

tra nsmissible in semen. 

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Health Certificate No ...................... 

11.4 . Where reference is made to health tests, the following tests were carried out: 

11.4 .1 . The cervicaltest for bovine tuberculosis described in the World Organization for Animal Health (WOAH, 

formerly OIE) Manual for Diagnostic Tests and Vaccines for Terrestrial Animals; 

11.4 .2 . SELECT ONE for brucellosis testing: 

 Buffered brucella antigen card test. 

 Rose bengal test. 

 Buffered plateagglutination test. 

 Indirect ELISA test for bovine brucellosis. 

 Competitive ELISA test for bovine brucellosis. 

 SELECT if this was performed: In accordance with the WOAH Manual of Diagnostic Tests and 

Vaccines for Terrestrial Animals, under the condition that samples that react positively were retested 

with negative results using a suitable confirmatory test such as the complement fixation test; 

11.4 .3 . SELECT ONE for bovine genital campylobacteriosis (Campylobacter fetus ssp. venerealis) with negative 

testing results: 

 A polymerase chain reaction (PCR) test. 

 Culture of preputial smegma. 

Note: The immunofluorescent antibody test may be used only as a screening test under the condition that 

samples that react positively must be retested using a suitable confirmatory test such as a PCR or culture of 

preputial smegma with negative results; 

11.4 .4 . SELECT ONE for trichomoniasis (Trichomonas foetus) with negative results; 

 PCR test. 

 Microscopic examination. 

 Culture of preputial smegma. 

11.4 .5 . SELECT ONE for epizootic hemorrhagic disease (EHD). 

 The animals originate from a Member State where no cases of EHD have been reported within the 

previous 12 months, and where no serological evidence of EHD infection exists; OR 

 The following serotypes of EHD exist:_______and animals were tested on two occasions by an agar gel 

immunodiffusion test (AGID) with negative results; OR 

 Testing was by competitive enzyme-linked immunosorbent assay (C-ELISA) AND a whole-blood PCR 

test for all the above-listed serotypes of EHD, with negative results using blood samples taken prior to, 

and not less than 21 days following collection of the semen (the two samples may not be taken more than 

12 months apart). OR 

 Testing was by competitive enzyme-linked immunosorbent assay (C-ELISA) AND a virus neutralization 

test (VNT) for all the above-listed serotypes of EHD, with negative results using blood samples taken 

prior to, and not less than 21 days following collection of the semen (the two samples may not be taken 

more than 12 months apart). 

11.4 .6 . The donor bull: 

 SELECT ONE: Wastested for the bluetongue virus (BTV) group on blood serum performed prior to the 

first day of semen collection, at least every 60 days during the collection period, and between 21 and 60 

days after semen collection, with negative results; 

 AGID test. 

 ELISA test. 

 OR (SELECT ONE) 

 Wastested with a whole blood PCR test for BTV group with one negative test at the 

beginning and conclusion of the collection period, and at least every 28 days during the 

period of semen collection. 

 Wastested with a whole-bloodvirusisolation test forBTVgroupwith one negative test at the 

beginning and conclusion of the collection period, and at least every 7 days during the period 

of semen collection. 

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Health Certificate No ...................... 

11.5 . 

11.6 . 

11.7 . 

11.8 . 

11.9 . 

11.10 . 

11.11 . 

The semen was collectedand processed under my supervision and placed in individual ampules or straws 

which were permanently marked with the name of the donor, his registration number, or the collectioncode; 

Semen collection equipment which came into contact with bulls, or their secretions and excretions was 

thoroughly disinfected after each use, and good laboratory practices were followed during collection and 

processing of semen in order to minimize the possible introduction of microbialcontamination; 

Antibiotics were addedto thesemenand semen extender in amounts and combinations consistent with the 

standards described in “Certified Semen Services (CSS) Minimum Requirements for Disease Control of Semen 

Produced for AI,” Appendix I, website: 202112136CSSMinReq Ja n2021-ENG_FINAL_v_4.pdf (naab-css.org). 

No biologicalproducts other than frozensemen or embryos qualified for shipment to the United States were 

present in the containers prior to use for export of semen to the United States; 

The storage and shipping containers are either new or cleaned and disinfected; and 

Only virgin liquid nitrogen was used to export semen to the United States. 

For sexed semen: 

11.11.1 The semen collected and processed under my supervision was shipped to the semen sexing facility 

within the Member State of collection under seal or was maintained under the oversight of a center 

or officia l veterinarian. 

11.11.2 Note: thesemen sexing facility used to sex the semen is located in the Member State where the 

semen was collected. 

12.1. Date and place 12.2. Name and qualification of 

the Center Veterinarian 

12.3. Signa ture a nd stamp of the Center 

Veterinaria n 

(The signature and stamp must be a different 

color than that of the printed template text.) 

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Health Certificate No ...................... 

Section B (to be signed by the Official Veterinarian after the Center Veterinarian has signed) 

13. I, the undersigned Official Veterinarian of 

__________________________________________________________ (Insert name of Member State of the 

European Union where semen was collected) certify that: 

13.1 . The Member State where the semen was collected is considered by the USDA to be free of foot-and- mouth 

disease, as listed in 9 CFR Part 94 and other official publications, and was free of this disease at the time of semen 

collection; 

13.2 . The Member State where the semen was collected is free of contagious bovine pleuropneumonia; 

13.3 . The donor animals for the semen to be exported to the United States have been part of the national herd of the 

Member State where the semen was collected for a minimum of 60 days and are free from any movement or 

quarantine restrictions; 

13.4 . The semen collection center, hereinafter “SCC,” was approved by the competent authority of the Member 

State where the semen was collected; 

13.5 . Health tests required for export to the United States of bovid semen were performed by testing methods 

recognized by theWorld Organization for Animal Health (WOAH, formerly OIE) Manual of Diagnostic Tests 

and Vaccines for Terrestrial Animals, as acceptable for international trade; 

13.6 . The la boratory tests mentioned in 11.4.2. to 11.4.5.2. were carried outwith negative results in a laboratory 

approved by the competent veterinary services; 

13.7 . Ruminant products used in commercial semen extenders in the Member State where the semen was collected 

were sourced from countries considered by USDA to be free from foot-and mouth disease a s listed in 9 CFR 

Part 94 and other official publications; 

13.8 . The semen to be exported to the United States was maintained under lock and key or in the custody of the SCC 

veterinarian, and segregated from other semen of lesser health status, until it was placed in the shipping container 

and sealed with official seals of the Member State where the semen was collected; 

13.9 . None of the semen for export to the USA has been stored or transported in containers with semen produced 

under less than equivalent animal health conditions; 

13.10 . The integrity of thetotal shipmentand continuity of storage conditions for semen produced in different approved 

SCC units and collected in the Member State listed on this health certificate (delete as appropriate); 

13.11 The shipping containers were sealed with an approved seal from the competent authority of the exporting 

Member State, and the seal number is recorded on the health certificate; 

13.12 . The semen is routed directly to the United States from the Member State in which it was collected with no stops 

en route other than those provided on the USDA import permit; and 

13.13 . The Center Veterinarian that completed Section A of this certificate is authorized by the National 

Veterinary Service to perform this service. 

13.14 . For sexed semen: 

13.14 .1 The semen sexing laboratory used to sex the semen for export to the United States is located in the EU 

Member State where the semen was collected or was imported from the United States meeting all EU 

import requirements. The semen collection center is under the supervision of an approved Center 

Veterinarian and is regularly inspected and approved in accordance with EU Directive 88/407/EEC and 

updated in Regulation (EU) 2016/429 or updated in Regulation (EU) 2016/429. The sexing facility 

followed a United States Department of Agriculture approved “Cleaning and Disinfection Standard 

Operating Protocol” while processing this semen for export to the United States. 

13.14 .2 The integrity of this shipmentwas maintained through thesemen sexing process and no semen from 

other donors was mixed with semen that originated from the animals listed in Part C. 

14.1. Date and place 14.2. Name and qualification of the 

Official Veterinarian 

14.3. Signature and stamp of the Official 

Veterinaria n 

(The signature and stamp must be a different color 

than that of the printed template text.) 

This certificate is valid for 30 days. Page 1/2024 5 of 5