CHAPTER 9.X. **INFECTION WITH DECAPODIRIDESCENT VIRUS 1**

Article 9.X.1.

For the purposes of the *Aquatic Code*, infection with decapod iridescent virus 1 means *infection***with the *pathogenic agent**Decapod iridescent virus 1* (DIV1), of the Genus *Decapodiridovirus* and the Family *Iridoviridae*. Information on methods for *diagnosis*is provided in the *Aquatic Manual*.

Article 9.X.2.

**Scope**

The recommendations in this chapter apply to the following species that meet the criteria for listing as susceptible in accordance with Chapter 1.5. [white-leg shrimp (*Penaeus vannamei)*, giant tiger prawn (*Penaeus monodon)*, red claw crayfish (*Cherax quadricarinatus*), giant freshwater prawn (*Macrobrachium rosenbergii*), red swamp crayfish (*Procambarus clarkii*), oriental river prawn (*Macrobrachium nipponense*) and ridgetail white prawn (*Exopalaemon carinicauda*)] (under study).

Article 9.X.3.

**Measures for the importation or transit of aquatic animal products for any purpose regardless of the infection with DIV1 status of the exporting country, zone or compartment**

The *aquatic animal products* listed below have been assessed as meeting the criteria for safety of *aquatic animal products* in accordance with Article 5.4.1.**When authorising the importation or transit of these*aquatic animal products*, C*ompetent****Authorities***should not require any conditions related to DIV1, regardless of the infection with DIV1 status of the *exporting****country*, *zone***or *compartment*:

1)	[cooked, pasteurised or retorted *aquatic animal**products* that have been subjected to a heat treatment sufficient to attain a core temperature of at least 56°C for at least 30 minutes, or a time/temperature equivalent that inactivates DIV1;

2)	crustacean *meal***that has been subjected to a heat treatment sufficient to attain a core temperature of at least 56°C for at least 30 minutes, or a time/temperature equivalent that inactivates DIV1; 

3)	crustacean oil;

4)	chemically extracted chitin.] (under study)

Article 9.X.4.

**Requirements for self-declaration of freedom from infection with DIV1**

A Member Country may make a self-declaration**of freedom**from infection with DIV1 for the entire country, a *zone* or a *compartment* in accordance with the provisions of Articles 9.X.5. to 9.X.8., as relevant. The self-declaration of freedom must be made in accordance with other relevant requirements of the *Aquatic Code,* including that the Member Country meet the following conditions:

1)	complies with the provisions of Chapter 3.1.; and

2)	uses appropriate methods of *diagnosis*, as recommended in the *Aquatic Manual*; and

3)	meets all requirements of Chapter 1.4. that are relevant to the self-declaration of freedom.

Article 9.X.5. 

**Country free from infection with DIV1**

If a country shares water bodies with other countries, it can only make a self-declaration  of freedom from infection with DIV1 if all shared water bodies are within countries or *zones* declared free from infection with DIV1 (see Article 9.X.6.).

As described in Article 1.4.X., a Member Country may make a self-declaration of freedom from infection with DIV1 for its entire *territory* if:

1)	none of the *susceptible species* referred to in Article 9.X.2. are present and *basic biosecurity conditions* have been continuously met for at least the last [six] months;

OR

2)	there has been no occurrence of infection with DIV1 for at least the last [ten] years, and:

a)	the Member Country can demonstrate that conditions are conducive to the clinical expression of infection with DIV1, as described in the corresponding chapter of the *Aquatic Manual*; and 

b)	*basic biosecurity conditions***as described in Chapter 1.4. have been continuously met for at least the last [ten] years; 

OR

3)	*targeted surveillance*, as described in Chapter 1.4., has been in place for at least the last [two] years without detection of DIV1, and *basic biosecurity conditions* have been continuously met for at least [one] year prior to commencement of *targeted surveillance*;

OR

4)	it previously made a self-declaration of freedom from infection with DIV1 and subsequently lost its free status due to the detection of DIV1 but the following conditions have been met:

a)	on detection of DIV1, the affected area was declared an *infected zone* and a *protection zone* was established; and

b)	infected populations within the *infected zone* have been killed and disposed of by means that minimise the likelihood of further transmission of DIV1, and the appropriate *disinfection* procedures (as described in Chapter 4.4.) have been completed followed by fallowing as described in Chapter 4.7.; and

c)	previously existing *basic biosecurity conditions* have been reviewed and modified as necessary and have continuously been in place since eradication of infection with DIV1; and

d)	*targeted surveillance*, as described in Chapter 1.4., has been in place for i) at least the last [two] years in  wild or farmed *susceptible species*without detection of DIV1 or ii) at least the last [one] year without detection of DIV1 if affected *aquaculture establishments* were not epidemiologically connected to wild populations of *susceptible species*.

In the meantime, part or all of the country, apart from the *infected*and *protection zones,* may be declared a free *zone* provided that such a part meets the conditions in point 2 of Article 9.X.6.

Article 9.X.6.

**Zone free from infection with DIV1**

If a *zone* extends over the *territory* of more than one country, it can only be declared a *zone* free from infection with DIV1 if all of the relevant *Competent Authorities* confirm that all relevant conditions have been met.

As described in Article 1.4.X., a Member Country may make a self-declaration of freedom from infection with DIV1 for a *zone* within its *territory* if:

1)	none of the *susceptible species* referred to in Article 9.X.2. are present and *basic biosecurity conditions* have been continuously met for at least the last [six] months;

OR

2)	there has been no occurrence of infection with DIV1 for at least the last [ten] years, and;

a)	the Member Country can demonstrate that conditions are conducive to the clinical expression of infection with DIV1, as described in Article 1.4.8. of Chapter 1.4.; and 

b)	*basic biosecurity conditions***as described in Chapter 1.4. have been continuously met for the *zone* for at least the last [ten] years;

OR

3)	*targeted surveillance*, as described in Chapter 1.4., has been in place in the *zone* for at least the last [two] years without detection of DIV1, and *basic biosecurity conditions* have been continuously met for at least [one] year prior to commencement of *targeted surveillance*; 

OR

4)	it previously made a self-declaration of freedom for a *zone* from infection with DIV1 and subsequently lost its free status due to the detection of DIV1 in the *zone* but the following conditions have been met:

a)	on detection of DIV1, the affected area was declared an *infected zone* and a *protection zone* was established; and

b)	infected populations within the *infected zone* have been killed and disposed of by means that minimise the likelihood of further transmission of DIV1, and the appropriate *disinfection* procedures (as described in Chapter 4.4.) have been completed followed by fallowing as described in Chapter 4.7.; and

c)	previously existing *basic biosecurity conditions* have been reviewed and modified as necessary and have continuously been in place since eradication of infection with DIV1; and

d)	*targeted surveillance*, as described in Chapter 1.4., has been in place for at least the last [two] years without detection of DIV1.

Article 9.X.7.

**Compartment free from infection with DIV1**

As described in Article 1.4.X., a Member Country may make a self-declaration of freedom**from infection with DIV1 for a***compartment* within its *territory* if:

1)	*targeted surveillance*, as described in Chapter 1.4., has been in place in the *compartment* for at least the last [two] years without detection of DIV1, and:

a)	*basic biosecurity conditions* have been continuously met for at least [one] year prior to commencement of *targeted surveillance*;

OR

2)	it previously made a self-declaration of freedom for a *compartment* from infection with DIV1 and subsequently lost its free status due to the detection of DIV1 in the *compartment* but the following conditions have been met:

a)	all *aquatic animals* within the *compartment* have been killed and disposed of by means that minimise the likelihood of further transmission of DIV1, the appropriate *disinfection* procedures (as described in Chapter 4.4.) have been completed, and the *compartment* has been fallowed as described in Chapter 4.7.; and

b)	previously existing *basic biosecurity conditions*, including the *compartment**biosecurity plan*, have been reviewed and modified as necessary and have continuously been in place from the time of restocking with animals from an approved pathogen free source in accordance with the requirements of Articles 9.X.9. and 9.X.10. as appropriate; and

c)	*targeted surveillance*, as described in Chapter 1.4., has been in place for at least the last [one] year without detection of DIV1.

Article 9.X.8.

**Maintenance of free status**

A country, *zone* or *compartment* that is declared free from infection with DIV1 following the provisions of Articles 9.X.4. to 9.X.7. (as relevant) may maintain its status as free from infection with DIV1 provided that the requirements described in Article 1.4.15. are continuously maintained.

Article 9.X.9.

**Importation of aquatic animals or aquatic animal products from a country, zone or compartment declared free from infection with DIV1**

When importing *aquatic animals*of a species referred to in Article 9.X.2., or *aquatic animal products*derived thereof, from a country, *zone*or *compartment*declared free from infection with DIV1, the *Competent Authority*of the *importing****country***should require that the consignment be accompanied by an *international****aquatic****animal****health****certificate***issued by the *Competent****Authority***of the *exporting****country*. The *international****aquatic****animal****health****certificate***should state that, on the basis of the procedures described in Articles 9.X.5.or 9.X.6.(as applicable) and 9.X.7.,the place of production of the *aquatic animals*or *aquatic animal products*is a country, *zone*or *compartment*declared free from infection with DIV1.

The *international aquatic animal health certificate*should be in accordance with the Model Certificate in Chapter 5.11. This article does not apply to *aquatic animal products*listed in Article 9.X.3.

Article 9.X.10.

**Importation of aquatic animals for aquaculture from a country, zone or compartment not declared free from infection with DIV1**

When importing, for *aquaculture*, *aquatic animals*of a species referred to in Article 9.X.2. from a country, *zone*or *compartment***not declared free from infection with DIV1, the *Competent****Authority***of the *importing****country***should assess the *risk*in accordance with Chapter 2.1. and consider the *risk*mitigation measures in points 1 and 2 below.

1)	If the intention is to grow out and harvest the imported *aquatic animals*, consider applying the following:

a)	the direct delivery to and lifelong holding of the imported *aquatic animals*in a *quarantine*facility; and

b)	before leaving *quarantine***(either in the original facility or following biosecure transport to another *quarantine***facility) the *aquatic****animals***are killed and processed into one or more of the *aquatic****animal****products***referred to in Article 9.X.3. or other products authorised by the *Competent Authority*; and

c)	the treatment of all transport water, equipment, effluent and waste materials to inactive DIV1 in accordance with Chapters 4.4., 4.8. and 5.5.

OR

2)	If the intention is to establish a new stock for *aquaculture*, consider applying the following:

a)	In the *exporting****country*:

i)	identify potential source populations and evaluate their *aquatic animal*health records;

ii)	test source populations in accordance with Chapter 1.4.and select a founder population (F-0) of *aquatic**animals*with a high health status for infection with DIV1.

b)	In the *importing****country*:

i)	import the F-0 population into a *quarantine***facility;

ii)	test the F-0 population for DIV1 in accordance with Chapter 1.4. to determine their suitability as broodstock;

iii)	produce a first generation (F-1) population in *quarantine*;

iv)	culture the F-1 population in *quarantine***for a duration sufficient for, and under conditions that are conducive to, the clinical expression of infection with DIV1, and sample and test for DIV1 in accordance with Chapter 1.4. of the *Aquatic Code*and Chapter X.X.6. of the *Aquatic****Manual*;

v)	if DIV1 is not detected in the F-1 population, it may be defined as free from infection with DIV1 and may be released from *quarantine*;

vi)	if DIV1 is detected in the F-1 population, those animals should not be released from *quarantine*and should be killed and disposed of in a biosecure manner in accordance with Chapter 4.7.

Article 9.X.11.

**Importation of aquatic animals or aquatic animal products for processing for human consumption from a country, zone or compartment not declared free from infection with DIV1**

When importing, for processing for human consumption, *aquatic animals*of a species referred to in Article 9.X.2., or *aquatic****animal****products***derived thereof, from a country, *zone***or *compartment***not declared free from infection with DIV1, the *Competent Authority*of the *importing country*should assess the *risk*and, if justified, require that:

1) the consignment is delivered directly to, and held in, *quarantine*or containment facilities until processing into one of the products referred to in Article 9.X.3.or in point 1 of Article 9.X.12.,or other products authorised by the *Competent Authority*; and

2) all water (including ice), equipment, *containers*and packaging material used in transport are treated to ensure inactivation of DIV1 or disposed of in a biosecure manner in accordance with Chapters 4.4.,4.8.and 5.5.;and

3) all effluent and waste materials are treated to ensure inactivation of DIV1 or disposed of in a biosecure manner in accordance with Chapters 4.4. and 4.8.

For these *aquatic animals*or *aquatic animal products*Member Countries may wish to consider introducing internal measures to address the *risks***associated with the *aquatic****animal***or *aquatic****animal****product***being used for any purpose other than for human consumption.

Article 9.X.12.

**Importation of aquatic animals or aquatic animal products intended for uses other than human consumption, including animal feed and agricultural, industrial, research or pharmaceutical use, from a country, zone or compartment not declared free from infection with DIV1**

When importing *aquatic animals*of a species referred to in Article 9.X.2., or *aquatic animal products*derived thereof, intended for uses other than human consumption, including animal *feed***and agricultural, industrial, research or pharmaceutical use, from a country, *zone*or *compartment*not declared free from infection with DIV1, the *Competent****Authority*of the *importing country*should require that:

1) the consignment is delivered directly to, and held in, *quarantine*or containment facilities until processed into one of the products referred to in Article 9.X.3.or other products authorised by the *Competent****Authority*; and

2) all water (including ice), equipment, *containers*and packaging material used in transport are treated to ensure inactivation of DIV1 or disposed of in a biosecure manner in accordance with Chapters 4.4.,4.8.and 5.5.;and

3) all effluent and waste materials are treated to ensure inactivation of DIV1 or disposed of in a biosecure manner in accordance with Chapters 4.4. and 4.8.

Article 9.X.13.

**Importation of aquatic animals intended for use in laboratories or zoos from a country, zone or compartment not declared free from infection with DIV1**

When importing, for use in laboratories or zoos, *aquatic****animals***of a species referred to in Article 9.X.2.from a country, *zone***or *compartment***not declared free from infection with DIV1, the *Competent****Authority***of the *importing****country***should ensure:

1) the consignment is delivered directly to, and held in, *quarantine*facilities authorised by the *Competent Authority*; and

2) all water (including ice), equipment, *containers*and packaging material used in transport are treated to ensure inactivation of DIV1 or disposed of in a biosecure manner in accordance with Chapters 4.4.,4.8.and 5.5.;and

3) all effluent and waste materials from the *quarantine*facilities in the laboratories or zoos are treated to ensure inactivation of DIV1 or disposed of in a biosecure manner in accordance with Chapters 4.4.and 4.8.;and

4) the carcasses are disposed of in accordance with Chapter4.8.

Article 9.X.14.

**Importation (or transit) of aquatic animal products for retail trade for human consumption regardless of the infection with DIV1 status of the exporting country, zone or compartment**

1) [*Competent****Authorities***should not require any conditions related to DIV1, regardless of the infection with DIV1 status of the *exporting****country*, *zone***or *compartment*, when authorising the importation (or transit) of frozen crustaceans of the *susceptible species* in Article 9.X.2. (shell off, head off) that have been prepared and packaged for retail trade and comply with Article 5.4.2.] (under study)

Certain assumptions have been made in assessing the safety of the *aquatic animal products*mentioned above. Member Countries should refer to these assumptions at Article 5.4.2.and consider whether the assumptions apply to their conditions.

For these *aquatic animal products***Member Countries may wish to consider introducing internal measures to address the *risks*associated with the *aquatic animal product*being used for any purpose other than for human consumption.

2) When importing *aquatic animal products*, other than those referred to in point 1 above, derived from a species referred to in Article 9.X.2. from a country, *zone*or *compartment*not declared free from infection with DIV1, the*Competent Authority***of the *importing country***should assess the *risk***and apply appropriate *risk***mitigation measures.

___________________________

Annex 11

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