An Equal Opportunity Provider and Employer

CENTER FOR VETERINARY BIOLOGICS NOTICE NO. 16-02

TO: Biologics Licensees, Permittees, and Applicants

Directors, Center for Veterinary Biologics

Veterinary Services Leadership Team

FROM: Byron Rippke

Director

 

SUBJECT: Availability of Avian Influenza Isolate and Sequence Information

I. PURPOSE

The purpose of this notice is to furnish interested parties with the genetic sequences

from the H7N8 highly pathogenic avian influenza (HPAI) virus and low pathogenic 

(LPAI) viruses isolated from turkeys in Indiana on January 15, 2016. The notice is 

also to inform interested parties that the HPAI isolate will be available for purchase 

from the National Veterinary Services Laboratories (NVSL) by mid-February 2016.

II. BACKGROUND

The National Veterinary Services Laboratories confirmed H7N8 HPAI in a 

commercial turkey flock experiencing significant mortality in Dubois County, 

Indiana, on January 15, 2016. Subsequent H7N8 detections were found to be H7N8 

LPAI. Based upon the full genome sequence, the index HPAI case and subsequent 

LPAI viruses detected in turkeys are all of North American wild bird lineage with 

high similarity across all eight gene segments to other wild bird viruses collected 

from Midwest and western states between 2011 and 2013. 

North American H7N8 LPAI virus has been detected previously in wild bird

surveillance in the United States, but this is the first instance of H7N8 HPAI virus 

detection in any species.

Further information on the H7N8 findings is available in the Newsroom 2016

section at https://www.aphis.usda.gov/wps/portal/aphis/newsroom/news.

III. ACTION

Veterinary Services is making the HPAI genomic sequence, as well as the HPAI 

virus courtesy of the Indiana State Board of Animal Health, available to facilities to 

encourage disease research and development to benefit the U.S. poultry industry. 

The complete genomic sequence for the HPAI can be found in the attached file. 

Sequence information has also been submitted to GenBank and will be available for 

both HPAI and LPAI viruses soon under accessions as follows: H7N8 HPAI 

KU558903-8910; H7N8 LPAI KU585905-12 and KU585913-20. 

Animal and Plant

Health Inspection

Service

Veterinary Services

Center for Veterinary 

Biologics

1920 Dayton Avenue

PO Box 844

Ames, IA 50010

(515) 337-6100
CENTER FOR VETERINARY BIOLOGICS NOTICE NO. 16-XX

Page 2

 An Equal Opportunity Provider and Employer

Available isolate:

• A/turkey/Indiana/16-001403-1/2016 – courtesy of the Indiana State Board of 

Animal Health

Biologics firms that wish to obtain samples of these isolates for research purposes should:

A. Contact the NVSL Diagnostic Virology Laboratory at (515) 337-7551 for 

guidance regarding the appropriate forms and payments to submit.

B. Submit the completed forms and the payment associated with the order by one of the 

following methods:

1. Mail: NVSL User Fees, Box 844, Ames, Iowa 50010 

2. Email: NVSL_concerns@aphis.usda.gov. 

The NVSL will inform customers when the virus is ready to ship.

C. Note that the HPAI isolate must be transferred in compliance with the Select 

Agent Regulations. No Material Transfer Agreement will be required. This isolate is 

a not pre-tested Master Seed; it is being made available for research and 

development. The availability of this isolate does not infer any impending change in 

vaccination policy.

IV. IMPLEMENTATION/ APPLICABILITY

The HPAI sequence is posted in this announcement, and the referenced reagent will be

available from the NVSL no later than February 15, 2016. 
>Seq1 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 1, polymerase 

PB2 (PB2) gene, complete cds.

ATGGAGAGAATAAAAGAATTAAGAGATCTAATGTCACAGTCTCGCACTCGCGAGATACTAACCAAAACCACTGTT

GACCACATGGCCATAATCAAAAAGTACACATCGGGAAGGCAAGAGAAGAACCCCGCACTCAGGATGAAATGGAT

GATGGCAATGAAATATCCAATCACAGCAGATAAGCGAATAATGGAAATGATCCCTGAAAGGAATGAACAAGGGC

AAACCCTCTGGAGCAAAACAAACGATGCCGGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGA

ATAGGAATGGACCAACAACAAGTACAATTCACTACCCAAAGGTATATAAAACTTATTTCGAAAAAGTTGAAAGATT

GAAACACGGGACCTTTGGCCCTGTACACTTCAGAAACCAAGTTAAGATAAGACGGAGAGTCGACATAAACCCGGG

CCATGCTGACCTCAGTGCCAAAGAGGCGCAGGATGTAATCATGGAAGTTGTCTTTCCAAATGAAGTGGGAGCGAG

AATACTGACATCGGAGTCACAACTGACAATAACAAAGGAGAAGAAGGAAGAACTCCAGGACTGCAAAATCGCCC

CTCTGATGGTTGCATACATGCTAGAAAGAGAGTTGGTCCGCAAGACGAGGTTTCTCCCAGTGGCTGGTGGAACAA

GCAGTGTCTACATTGAGGTGCTGCATCTGACCCAGGGGACATGCTGGGAGCAGATGTATACTCCAGGAGGAGAA

GTGAGAAACGATGATGTAGACCAGAGCTTGATTATTGCTGCCAGGAACATAGTAAGAAGAGCAACAGTATCAGCA

GACCCACTAGCGTCTCTATTGGAGATGTGCCACAGCACACAAATTGGGGGAATAAGGATGGTAGACATTCTTCGG

CAAAATCCAACAGAGGAACAAGCCGTGGACATATGCAAGGCAGCAATGGGCCTGAGGATTAGCTCATCTTTCAGC

TTCGGTGGATTCACTTTTAAAAGAACAAGTGGATCGTCAGTCAAAAGAGAAGAAGAAGTGCTTACGGGCAACCTT

CAGACATTGAAAATAAGAGTACATGAGGGATATGAAGAGTTCACAATGGTTGGAAGAAGAGCAACGGCCATTCT

CAGGAAGGCAACCAGAAGGCTGGTCCAGCTAATAGTAAGTGGAAGAGACGAGCAGTCAATTGCTGAAGCAATAA

TTGTGGCCATGGTATTCTCACAAGAGGACTGCATGATTAAGGCAGTTCGAGGTGACCTGAATTTTGTCAATAGGGC

GAACCAGCGGCTGAACCCAATGCATCAACTCTTGCGACACTTCCAAAAGGATGCAAAAGTGCTTTTCCAAAATTGG

GGAATTGAACCCATTGACAATGTTATGGGAATGATCGGGATATTGCCCGACATGACCCCAAGTACTGAAATGTCG

CTGAGGGGAATAAGAGTCAGTAAGATGGGAGTAGATGAATACTCCAGTACAGAGCGGGTAGTAGTAAGCATCGA

CCGATTTTTAAGAGTTCGAGACCAACGGGGGAACGTACTATTGTCACCCGAGGAAGTCAGCGAGACACAAGGAAC

GGAGAAATTGACAATCACTTATTCGTCATCAATGATGTGGGAGATCAATGGTCCTGAGTCGGTGTTGGTCAATACC

TATCAGTGGATAATCAGAAACTGGGAAACTGTAAAAATTCAATGGTCACAGGATCCCACAATGTTGTATAATAAGA

TGGAGTTCGAGCCATTTCAGTCTCTAGTCCCTAAGGCAGCCAGAGGTCAATACAGTGGGTTCGTGAGGACACTATT

CCAGCAAATGCGAGATGTGCTTGGAACATTTGACACTGTTCAGATAATAAAACTCCTCCCCTTTGCTGCTGCCCCAC

CGGAGCAAAGTAGGATGCAGTTCTCCTCCCTGACTGTGAATGTGAGAGGATCAGGAATGAGAATACTGGTAAGA

GGCAATTCCCCGGTGTTCAATTACAACAAGGCCACCAAGAGGCTCACAGTTCTCGGGAAAGATGCAGGTGCATTG

ACCGAAGATCCAGATGAAGGCACAGCTGGAGTGGAGTCTGCTGTTTTAAGAGGATTCCTCATTTTGGGCAAAGAA

GACAAGAGATATGGCCCAGCACTGAGCATCAATGAGCTGAGCAATCTTGCAAAGGGAGAGAAGGCTAATGTGCT

AATTGGGCAAGGAGACGTAGTGTTGGTGATGAAACGGAAACGGAACTCTAGCATACTTACTGACAGCCAGACAG

CGACCAAAAGAATTCGGATGGCCATCAATTAGTGTCGAA

>Seq2 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 2, polymerase 

PB1 (PB1) gene, complete cds.

ATGGATGTCAATCCGACTTTACTTTTCTTGAAAGTTCCAGCGCAAAATGCCATAAGCACCACATTCCCGTATACTGG

AGATCCTCCATACAGCCATGGAACAGGAACAGGATATACCATGGACACGGTTAACAGAACACATCAATATTCAGA

AAAGGGGAAATGGACAACAAACTCAGAGACTGGAGCACCTCAGCTCAATCCAATTGATGGACCATTGCCTGAGGA

CAATGAACCAAGTGGATATGCACAAACAGACTGTGTCCTTGAAGCAATGGCTTTTCTTGAAGAGTCCCACCCAGGA

ATCTTTGAAAACTCGTGTCTTGAAACGATGGAAGTTGTTCAACAAACAAGAGTGGACAAATTGACCCAAGGTCGCC

AGACCTATGATTGGACATTAAACAGGAATCAGCCGGCTGCAACTGCATTAGCCAATACTATAGAGGTCTTCAGATC
GAACGGTCTTACAGCTAATGAATCAGGAAGACTAATAGATTTTCTCAAAGATGTGATGGAATCAATGGATAAAGA

GGAAATGGAAATAACAACGCATTTCCAAAGGAAAAGAAGAGTGAGAGACAACATGACCAAGAAAATGGTCACAC

AAAGGACAATAGGAAAGAAGAAGCAGAGGCTAAACAAAAAGAGCTATCTAATAAGAGCATTAACACTGAACACA

ATGACAAAAGACGCCGAAAGAGGCAAATTGAAGAGAAGAGCAATTGCAACACCCGGAATGCAAATCAGAGGGTT

TGTGTACTTTGTTGAAACATTAGCAAGGAGCATTTGTGAAAAGCTCGAACAATCTGGACTCCCAGTTGGAGGCAAT

GAAAAGAAGGCTAAACTGGCAAATGTTGTGAGAAAAATGATGACTAATTCGCAAGACACAGAGCTCTCTTTCACA

ATCACTGGAGACAACACCAAATGGAATGAAAACCAGAACCCTAGGATGTTTCTGGCAATGATAACGTATATAACA

AGAAACCAACCTGAATGGTTCAGGAATGTCTTGAGTATTGCACCTATAATGTTCTCAAATAAAATGGCAAGACTAG

GGAAAGGATACATGTTCGAAAGTAAGAGCATGAAGCTTCGAACACAAATACCGGCAGAAATGTTAGCAAGCATT

GATCTGAAGTATTTCAATGAGTCAACACGGAAGAAAATAGAAAAGATAAGACCTCTTCTAATAGATGGTACAGCC

TCATTGAGCCCTGGAATGATGATGGGCATGTTCAACATGTTGAGTACAGTTCTGGGAGTTTCGATTCTAAACCTAG

GGCAAAAGAGGTACACCAAAACAACATACTGGTGGGACGGACTCCAATCCTCTGATGACTTTGCCCTCATAGTGA

ATGCTCCGAATCATGAGGGAATACAAGCAGGTGTAGACAGATTCTACAGAACCTGCAAGCTGGTCGGAATCAACA

TGAGCAAAAAGAAGTCCTACATAAACAGAACAGGGACATTTGAATTCACAAGTTTTTTCTACCGCTATGGATTCGT

AGCCAACTTCAGCATGGAGTTGCCCAGCTTTGGAGTGTCCGGGATCAATGAATCTGCAGACATGAGCATTGGAGT

AACAGTGATAAAAAACAACATGATCAACAATGATCTTGGACCAGCAACAGCCCAAATGGCTCTTCAGCTATTCATC

AAGGATTACAGATACACGTATCGGTGTCACAGAGGAGACACACAAATTCAGACAAGGAGGTCATTCGAGCTGAA

GAAGCTATGGGAACAAACCCGTTCAAAGGCAGGACTACTGGTCTCCGATGGAGGGCCAAATCTATACAATATCCG

GAATCTCCACATTCCGGAAGTTTGCCTGAAATGGGAGCTAATGGATGAAGACTATCAGGGAAGGCTTTGTAACCC

CCTGAATCCGTTTGTCAGCCACAAAGAGATAGAGTCTGTAAACAATGCTGTAGTGATGCCAGCTCATGGTCCAGCC

AAGAGCATGGAATATGATGCTGTTGCCACCACGCATTCCTGGATACCTAAGAGGAACCGCTCCATTCTCAATACAA

GCCAAAGGGGAATCCTTGAAGACGAACAGATGTATCAAAAGTGCTGCAATTTATTCGAGAAATTCTTCCCTAGCAG

TTCATACAGGAGGCCGGTTGGAATTTCCAGCATGGTGGAGGCCATGGTTTCTAGGGCCCGAATTGATGCGCGAAT

TGACTTCGAATCTGGACGGATCAAGAAGGAGGAGTTTGCTGAGATCATGAAGATCTGTTCCACCATTGAAGAGCT

CAGACGGCAGAAATAGTGAATTTGGCTTGTCC

>Seq3 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 3, polymerase 

PA (PA) gene, complete cds.

ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGG

GAAGATCCGAAAATCGAAACTAACAAGTTCGCTGCAATATGCACTCATTTGGAAGTCTGTTTCATGTATTCGGATTT

CCATTTCATTGATGAACGGGGCGAATCAATAATTGTGGAATCTGGTGATCCAAATGCATTACTGAAGCATCGATTT

GAGATAATTGAAGGAAGAGACAGAACAATGGCCTGGACAGTGGTGAATAGCATCTGCAACACCACGGGGGTCGA

GAAGCCTAAGTTCCTTCCGGATCTGTATGATTACAAGGAGAACCGATTCATTGAAATTGGGGTGACACGGAGAGA

GGTCCATATATATTACCTAGAGAAAGCCAACAAGATAAAATCCGAGAAGACACACATTCACATCTTCTCATTCACT

GGAGAAGAGATGGCCACCAAAGCAGACTACACCCTTGACGAAGAAAGCAGAGCAAGGATTAAAACCAGACTATT

CACTATAAGACAAGAGATGGCCAGCAGAGGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAAT

TGAAGAAAGATTTGAAATCACAGGAACCATGCGCAGGCTTGCCGACCAAAGTCTCCCACCGAACTTCTCCAGCCTT

GAAAACTTTAGAGCCTATGTGGATGGATTCGAACCGAACGGCTGCATTGAGGGCAAGCTTTCTCAAATGTCCAAA

GAAGTGAACGCCAGAATTGAGCCATTTTTGAAAACAACACCACGCCCTCTCAAATTGCCTGATGGGCCCCCTTGCT

CTCAGCGGTCAAAATTCTTGCTGATGGATGCCTTGAAACTAAGCATTGAAGACCCAAGTCATGAGGGAGAGGGGA

TACCGCTGTACGATGCAATTAAATGCATGAAGACATTTTTCGGCTGGAAAGAGCCCAATATAATCAAACCACATGA

AAAGGGCATAAACCCTAACTATCTCCTGGCTTGGAAGCAAGTGCTAGCAGAACTCCAGGGCCTTGAAAATGAAGA

GAAAATTCCAAAGACGAAGAACATGAAGAAAACAAGTCAATTAAAGTGGGCACTTGGCGAGAATATGGCACCAG
AAAAAGTGGACTTTGAGGATTGCAAGGATGTTGGCGATCTAAAACAGTATGATAGCGATGAGCCAGAGCCTAGAT

CGCTAGCAAGCTGGATCCAGAGTGAATTCAACAAGGCATGTGAATTGACCGACTCAAGCTGGATAGAACTGGATG

AAATAGGGGAAGATGTTGCTCCGATTGAACACATTGCAAGTATGAGGAGGAATTATTTCACAGCAGAAGTGTCCC

ATTGCAGGGCCACTGAATACATAATGAAAGGAGTCTACATAAATACAGCTCTGCTCAATGCATCTTGCGCGGCCAT

GGATGACTTCCAGCTGATTCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATG

GGTTCATCATAAAGGGAAGGTCTCATTTGAGGAATGATACCGATGTGGTAAATTTTGTAAGTATGGAGTTTTCTCT

CACCGACCCAAGGCTGGAACCACACAAATGGGAAAAGTACTGCGTTCTTGAGGTGGGAGATATGCTCCTGAGGAC

TGCAATAGGCCAAGTATCAAGACCCATGTTCCTGTATGTTAGGACCAACGGGACCTCCAAAATCAAGATGAAATG

GGGTATGGAGATGAGGCGTTGCCTTCTTCAGTCTCTTCAACAGATTGAAAGCATGATTGAGGCCGAGTCCTCAGTC

AAAGAAAAAGACATGACCAAAGAATTTTTTGAGAACAAGTCGGAAACGTGGCCAATTGGAGAGTCCCCCAGAGG

GGTAGAGGAAGGATCCATTGGGAAGGTATGCAGAACCCTGCTAGCAAAATCTGTGTTCAACAGTCTATATGCATC

CCCACAACTTGAGGGATTTTCAGCAGAATCGAgGAAACTGCTTCTCATTGTTCAGGCACTTAGGGACAACCTGGAA

CCTGGAACCTTCGATCTTGGAGGGCTATACGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA

ATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTGAAATAGTTGTGGCAATGCTACTATTTGCTATCCATAC

>Seq4 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 4, 

hemagglutinin (HA) gene, complete cds.

TACAAAATGAACACTCAAATTTTGGCACTCATTGCTTGTATGCTGATCGGAGCTAAAGGAGACAAAATATGTCTTG

GGCACCATGCTGTGGCAAATGGAACAAAAGTGAACACACTAACAGAGAGAGGAATCGAAGTGGTAAATGCCACA

GAAACGGTGGAGACTGCAAATATTAAGAAAATATGCACTCAGGGGAAAAGACCAACAGATCTGGGACAATGCGG

ACTTCTAGGAACCCTAATAGGACCTCCCCAATGCGATCAATTCCTGGAGTTTGACGCTGATTTAATAATTGAACGG

AGAGAAGGAACGGATGTGTGTTATCCTGGGAAGTTCACAAATAAAGAATCACTGAGGCAGATCCTCCGAGGGTC

AGGAGGAATTGATAAGGAATCAATGGGTTTCACTTATAGTGGAATAAGAACCAATGGGGCAACGAGTGCTTGCA

GAAGATCAGGTTCTTCCTTCTATGCGGAGATGAAGTGGTTATTGTCGAATTCAGACAATGCGGCTTTTCCCCAAAT

GACTAAGTCGTACAGAAATCCCAGGAGCAAACCAGCTCTAATAATTTGGGGAGTGCATCACTCTGGATCGGTTACT

GAACAGACCAAACTCTATGGGAGTGGAAACAAGTTGATAACAGTAGGAAGCTCGAAATACCAGCAGTCATTCACC

CCAAGCCCGGGGGCACGACCACAGGTGAATGGGCAATCAGGAAGGATTGATTTTCATTGGCTACTCCTTGACCCC

AACGACACAGTGACTTTCACTTTCAATGGGGCATTCATAGCTCCTGACAGAGCAAGTTTCCTTAGAGGGGAGTCAC

TAGGAGTTCAAAGTGACGTTCCTTTGGATTCTAGTTGCAAGGGGGATTGCTTCCACAATGGAGGTACGATAGTGA

GCTCACTGCCATTCCAGAACATCAACCCTAGAACAGTGGGAAAATGCCCTCGATATGTCAAACAGACAAGCCTCCT

CTTGGCTACAGGGATGAGGAACGTCCCAGAGAACCCCAAAAAAAGAAAGACCAGAGGTCTTTTTGGAGCGATTG

CTGGATTCATAGAGAATGGGTGGGAAGGCCTCATTGATGGATGGTACGGTTTCAGGCATCAAAATGCACAAGGA

GAAGGTACTGCAGCTGATTACAAAAGCACTCAATCTGCAATAGATCAGATCACAGGCAAATTGAATCGTCTAATTG

ACAAAACAAATCAGCAGTTTGAACTGATAGACAACGAATTCAGTGAAATAGAACAACAGATTGGGAATGTCATTA

ATTGGACACGAGATTCAATGACTGAAGTATGGTCGTATAATGCTGAACTGCTGGTAGCTATGGAAAATCAGCACA

CAATAGATCTTGCAGACTCAGAAATGAACAAACTTTACGAGCGTGTAAGGAAACAACTGAGGGAGAATGCTGAA

GAGGATGGGACTGGATGCTTTGAGATATTCCATAAGTGTGATGATCAATGCATGGAGAGCATAAGGAACAACACC

TACGACCATACCCAATACAGAGCAGAGTCATTGCAGAATAGAATACAGATAGACCCAGTGAAACTGAGTAGTGGA

TACAAAGACATAATCTTATGGTTTAGCTTCGGGGCATCATGTTTTCTTCTTCTAGCCATTGCAATGGGATTGGTCTT

CATTTGCATAAAGAATGGAAACATGCGGTGCACTATTTGTATATAGTTTGAGA
>Seq5 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 5, 

nucleoprotein (NP) gene, complete cds.

TCACTCACTGAGTGACATCCACATCATGGCGTCTCAAGGCACCAAACGATCTTACGAACAAATGGAAACTGGTGG

GGAACGCCAGAATGCCACTGAAATCAGAGCATCTGTTGGAAGAATGGTTGGCGGAATCGGGAGATTCTACATACA

GATGTGCACTGAGCTCAAACTCAGTGACTACGAAGGGAGACTGATCCAAAACAGCATTACCATAGAGAGGATGGT

TCTCTCAGCATTTGATGAGAGGAGAAACAAGTATCTGGAGGAGCATCCCAGTGCTGGGAAAGATCCCAAGAAGAC

TGGAGGCCCAATCTACAGAAGGAGAGATGGCAAATGGATGAGAGAGTTGATCCTATATGACAAAGAAGAGATCA

GAAGAATTTGGCGTCAAGCTAATAATGGGGAGGACGCAACTGCTGGTCTCACCCATATGATGATTTGGCATTCTA

ATCTGAATGATGCCACATACCAGAGAACAAGGGCACTTGTGCGTACTGGAATGGACCCCAGGATGTGCTCTCTGA

TGCAAGGCTCAACCCTCCCAAGGAGGTCTGGGGCTGCAGGAGCGGCAGTGAAAGGGGTTGGGACAATGGTGAT

GGAATTGATCCGGATGATCAAGCGAGGGATCAATGATCGGAATTTCTGGAGAGGCGAAAATGGACGGAGAACTA

GAATTGCCTACGAGAGAATGTGCAACATCCTCAAGGGAAAATTCCAAACAGCAGCACAACGAGCAATGATGGACC

AAGTGAGGGAAAGCCGGAATCCTGGGAACGCTGAAATTGAAGATCTCATCTTTCTCGCACGGTCTGCTCTCATTCT

GAGGGGATCAGTGGCTCATAAGTCCTGCCTGCCTGCTTGTGTGTATGGACTTGCTGTAGTCAGTGGATACGACTTT

GAGAGAGAGGGGTACTCTCTAGTCGGAATTGATCCTTTCCGTCTGCTCCAAAACAGCCAAGTCTTCAGTCTCATCA

GACCAAACGAAAATCCAGCGCACAAAAGTCAGCTGGTATGGATGGCATGCCACTCTGCAGCATTTGAAGATCTGA

GAGTGTCAAGCTTCATCAGAGGAACAAGAGTAGTCCCAAGAGGACAACTGTCCACCAGAGGAGTTCAGATTGCTT

CAAATGAGAACATGGAGACAATGGACTCCAATACTCTTGAATTGAGGAGCAGATACTGGGCTATAAGAACAAGAA

GCGGAGGGAACACCAACCAGCAGAGGGCATCTGCAGGACAAATCAGCGTACAGCCCACATTCTCTGTGCAGAGA

AACCTCCCATTCGAGAGAGCAACCATCATGGCAGCATTTACGGGAAACACTGAGGGCAGAACTTCAGACATGAGA

ACTGAGATCATAAGGATGATGGAAAATGCCAGACCTGAAGATGTGTCTTTCCAGGGGCGGGGAGTCTTCGAGCTC

TCGGACGAAAAGGCAACGAACCCGATCGTGCCTTCCTTTGACATGAGTAATGAAGGATCTTATTTCTTCGGAGACA

ATGCAGAGGAGTATGACAATTAAAG

>Seq6 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 6, 

neuraminidase (NA) gene, complete cds.

ATGAATCCAAATAAGAAAATAATAACCATTGGTTCAGTGTCCTTAGGATTGGTAGTCCTTAACATTCTCCTACATAT

AGTTAGCATTACAGTAACAGTGTTAGTTCTTCCTGGAAATGGAAATAACGGGAATTGCAATGAAACAGTCATTAGA

GAATACAATGAAACAGTAAGGATTGAGAAGGTAACACAATGGCACAATACCAATGTCATTGAGTATATAGAAAGG

CCAGAGAATGATCATTTCATGAACAATACAGAAGCATTGTGTGATGCCAAGGGGTTTGCACCCTTTTCCAAAGACA

ATGGAATAAGAATTGGATCGAGAGGTCATATTTTTGTTATAAGAGAACCATTTGTTTCTTGCTCTCCAACAGAGTG

CAGGACGTTCTTCCTTACTCAGGGTTCCTTACTCAATGACAAACACTCCAATGGCACAGTAAAAGACCGGAGCCCT

TATAGAACTCTAATGAGTGTAGAAATAGGGCAGTCACCCAATGTGTACCAGGCAAGGTTTGAGGCAGTAGCGTGG

TCAGCCACTGCATGTCATGATGGGAAGAAATGGATGACAATTGGAGTGACGGGCCCTGATGCCGAAGCAGTAGC

AGTAGTGCACTATGGGGGAATTCCTACTGATGTAATCAATTCCTGGGCAGGGGATATTCTAAGAACTCAGGAATC

ATCATGTACTTGCATTCAAGGTGAATGTTATTGGGTAATGACGGACGGACCAGCAAACAGACAAGCACAATATAG

AGCATTCAAGGCCAAGCAGGGGAAAATAGTTGGGCAAACTGAAATCAGTTTTAATGGAGGCCATATAGAAGAAT

GTTCATGCTACCCTAATGAAGGTAAAGTGGAATGTGTTTGTAGGGACAATTGGACTGGAACCAACAGACCAGTAT

TGGTGATTTCTCCAGATTTGTCTTACAGAGTCGGATACTTGTGTGCAGGTCTCCCCAGTGACACTCCAAGAGGAGA

AGATAGTCAGTTCACAGGATCCTGCACTAGCCCGATGGGAAATCAGGGATATGGAGTTAAGGGATTTGGATTCAG

GCAGGGCAATGATTTATGGATGGGAAGGACCATTAGCAGAACATCAAGATCAGGATTTGAAATCCTGAAAGTCAG

AAATGGCTGGGTACAAAATAGTAAAGAGCAGATCAAAAGGCAAGTTGTGGTCGATAACCTGAACTGGTCAGGAT
ACAGTGGTTCCTTCACACTACCGGTGGAGTTGACAAAAAGAAATTGTCTGGTTCCATGCTTTTGGGTTGAGATGAT

AAGGGGGAAGCCAGAAGAAAAGACTATATGGACCTCAAGTAGCTCCATTGTGATGTGTGGAGTAGACCATGAGA

TTGCCGACTGGTCATGGCACGATGGAGCTATTCTTCCTTTTGACATCGACAAGATGTAATTTACG

>Seq7 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 7, matrix 

protein 2 (M2) and matrix protein 1 (M1) genes, complete cds.

GAAAGATGAGTCTTCTAACCGAGGTAGAAACGTACGTTCTCTCTATCGTCCCGTCAGGCCCCCTCAAAGCCGAGAT

CGCGCAGAGACTTGAAGATGTGTTTGCAGGGAAAAACACCGACCTTGAGGCGCTCATGGAATGGCTAAAGACAA

GACCAATCCTGTCACCTCTGACTAAGGGGATTTTGGGATTTGTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACT

GCAGCGTAGACGCTTTGTCCAAAATGCCCTTAATGGGAATGGGGATCCAAACAACATGGACAGAGCAGTTAAACT

GTACAGGAAGCTTAAAAGGGAAATAACATTCCATGGGGCAAAAGAGGTGGCACTCAGTTATTCGACTGGTGCACT

TGCCAGCTGCATGGGCCTCATATACAACAGAATGGGGACGGTGACCACTGAGGTGGCATTTGGCCTGGTGTGTGC

CACATGTGAGCAGATTGCTGATTCCCAGCATCGGTCTCACAGACAAATGGTGACAACAACCAACCCGCTGATCAG

GCACGAGAACAGGATGGTACTGGCCAGTACTACGGCTAAGGCCATGGAACAAATGGCAGGATCAAGTGAGCAGG

CAGCAGAGGCCATAGAGGTTGCTAGTCAGGCTAGGCAGATGGTGCAGGCAATGAGGACCATTGGGACTCATCCT

AGCTCCAGTGCTGGTCTGAAAGATGATCTTCTTGAAAATTTGCAGGCCTACCAGAAACGGATGGGAGTGCAAATG

CAACGATTCAAGTGATCCTCTCGTTATTGCCGCAAGTATCATTGGGATCTTGCACTTGATATTGTGGATTCTTGATC

GCCTTTTCTTCAAATGCATTTATCGTCGCCTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGA

GTCTATGAGGGAAGAATATCGGCAGGAACAGCAGAGTGCTGTGGATGTTGACGATGGTCATTTTGTCAACATAGA

GCTGGAGTAA

>Seq8 [organism=Influenza A virus](A/turkey/Indiana/16-001403-1/2016(H7N8)) segment 8, nonstructural protein NS1 and non-structural protein NS2 (NS) gene, complete cds.

TACATAATGGATTCCAACACGATAACCTCGTTTCAGGTAGATTGCTATCTATGGCACATAAGGAAGCTGCTCAGCA

TGAGAGACATGTGTGATGCTCCCTTTGATGACAGACTCAGGAGAGATCAAAAGGCATTAAAGGGAAGAGGTAGC

ACACTTGGGCTCGATCTGCGAGTGGCCACAATGGACGGTAAAAAGATTGTTGAAGACATCCTGAAAAGTGAGACT

GATGAAAATCTCAAAATTGCAATTGCATCCAGCCCTGCTCCTCGGTATATTACCGATATGAGTATAGAGGAAATAA

GTAGAGAATGGTACATGCTCATGCCAAGACAGAAGATAACAGGGGGTCTGATGGTGAAAATGGACCAGGCAATC

ATGGATAAGAGAATAATACTCAAAGCAAACTTTTCTGTCCTATTTGATCAATTGGAAACATTAGTCTCACTGAGGG

CTTTCACCGACGATGGCGCCATTGTAGCTGAAATATCTCCCATTCCTTCTATGCCAGGACATTCTACAGAGGATGTC

AAAGATGCAATTGGAATCCTCATCGGTGGACTTGAATGGAATGATAACTCAATTCGAGCGTCTGAAAATATACAG

AGATATGCTTGGGGAATCCGTGATGAGAATGGGGGATCTCCATTCCCTCCAAAGCAGAAACGCCACATGGCGAGA

AGAGTTGAGTCAGAAGTTTGAAGAGATCAGATGGCTGATTGCAGAGTGCAGGAACATATTAACCAAAACTGAAA

ACAGTTTCGAGCAGATAACGTTCTTGCAAGCATTGCAACTCTTACTTGAAGTTGAGAGTGAGATAAGAACATTTTC

TTTTCAGCTTATTTAATACTAA