Koninkrijk der Nederlanden 

 GEZONDHEIDSCERTIFICAAT 

 (veterinair certificaat) certificaatnummer: 

 Page 1 of 4

Koninkrijk der Nederlanden Kingdom of the Netherlands Königreich der Niederlande Royaume des Pays-Bas Reino de los Países Bajos 

Ministerie van Economische 

Zaken 

Ministry of Economic Affairs Ministerium für Wirtschaft Ministère des Affaires 

économiques 

Ministerio de Economía 

Nederlandse Voedsel- en 

Warenautoriteit 

Netherlands Food and 

Consumer Product Safety 

Authority 

Niederländische Behörde für 

Ernährung und 

Produktsicherheit 

Autorité néerlandaise de 

surveillance des produits 

alimentaires et non 

alimentaires 

Autoridad holandesa de la 

seguridad Alimentaria y de los 

Productos de consumo 

Gezondheidscertificaat Health Certificate Gesundheitsbescheinigung Certificat Sanitaire Certificado Sanitario 

Document ref. : NVWA_CLH_B_US_rundersperma_EN-certificaatmodel 2.4.rtf 

HEALTH CERTIFICATE FOR EXPORT OF BOVINE SEMEN FROM FOOT-AND-MOUTH DISEASE-FREE MEMBER 

STATES OF THE EUROPEAN UNION TO THE UNITED STATES OF AMERICA 

I. IDENTIFICATION OF THE PRODUCTS 

Product no. 

Product 

(Name of the donor bull) Breed Age Identification number 

 

Batch no. Type of semen Packing (Number of straws) Date of collection Collection code 

 sexed / nonsexed(*)

Seal number :

II. ORIGIN OF THE PRODUCTS 

Product no. Approval no. semen collection centre Name and address semen collection centre 

Product no. Name and address of the semen sexing facility 

Name and address of exporter :

Place of loading :

Country of loading :

III. DESTINATION OF THE PRODUCTS

Means of conveyance :

Name and address of consignee :

IV. HEALTH ATTESTATION 

Section A (to be signed by the Centre Veterinarian) 

I, the undersigned Centre Veterinarian of the described semen collection centre, hereinafter “SCC”, certify 

that: 

1. All bovine animals in the above SCC were: 

 1.1. Established as residents only if admitted by a formal process of quarantine, observation, and 

testing as required by legislation in force, notably Annex B to Council Directive 88/407/EEC, as 

amended by Directive 2003/43/EC; 

 1.2. Admitted to the SCC herd only after having been proven free of brucellosis, tuberculosis, 

bovine genital campylobacteriosis and trichomoniasis; 

 1.3. Admitted to the SCC herd only after having been proven free of viremia from persistent bovine 

viral diarrhea virus infection before entry into the SCC resident herd; and 

 1.4. Were tested annually for brucellosis, tuberculosis, bovine genital campylobacteriosis, and 

trichomoniasis; 

 1.5. The semen for export to the United States was either collected prior to June 1, 2011; 

 Or(*) The semen in the consignment was collected after June 1, 2011 from donors that were 

negative to two serum neutralization tests (using a 1:8 cutoff titer) for Schmallenberg 

virus, with the first performed within 30 days prior to collection, and the second 

between 28 and 60 days after collection. Tests were performed in a laboratory 

approved by the national Competent Authority; 

2. In the SCC: 
certificaatnummer: 

 Page 2 of 4

Document ref. : NVWA_CLH_B_US_rundersperma_EN-certificaatmodel 2.4.rtf 

 2.1. The herd was tested for brucellosis, tuberculosis, bovine genital campylobacteriosis, and 

trichomoniasis in its entirety with negative results at the most recent herd test prior to the 

period of semen collection for export to the United States of America (USA); 

 2.2. No clinical or other evidence of brucellosis, tuberculosis, bovine genital campylobacteriosis, 

trichomoniasis or leptospirosis was found since the most recent herd test and prior to the 

embarkation of semen to the USA; 

 2.3. There was no evidence to indicate that the donors have been affected with tuberculosis or 

brucellosis during the 12 months prior to the collection of semen for export to the USA; 

 2.4. There was no clinical evidence of infection by bovine viral diarrhea virus, bluetongue virus, or

infectious bovine rhinotracheitis virus during the 60 days prior to and during the period of 

collection of semen for export to the USA; and 

 2.5. All bulls passed a testing program with negative result consistent with the Terrestrial Animal 

Health Code of the OIE (Article 4.5.5) or as outlined in Council Directive 88/407/EEC, as 

amended, to detect persistent testicular bovine viral diarrhea virus infection prior to semen 

release; 

3. Each donor bull for the semen described above: 

 3.1. Originated from a tuberculosis-free herd; 

 3.2. Was not corralled, pastured, or held with animals of lesser health status or under any 

restrictions which would make them ineligible to export semen to the USA during the 60 days 

prior to and during the period of collection of semen for export to the USA; 

 3.3. Was subjected with negative results to the test described in IV. 4.1 to IV. 4.4 within 6 months 

prior to collection or 6 months after collection of the semen described above; 

 3.4. Was subjected with negative results to the tests for bluetongue virus group (BTV) described in 

IV. 4.5; 

 3.5. Was inspected on the date of semen collection and found to be free of clinical signs of diseases

transmissible in semen; 

4. Where reference is made to health tests, the following tests were carried out: 

 4.1. The cervical test for bovine tuberculosis described in the OIE Manual for Diagnostic Tests and 

Vaccines for Terrestrial Animals; 

 4.2. Either a buffered brucella antigen test (card test, rose bengal test, or the buffered plate 

agglutination test), or an ELISA test for bovine brucellosis (indirect or competitive) in 

accordance with the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 

under the condition that samples that react positively were retested with negative results 

using a suitable confirmatory test such as the complement fixation test; 

 4.3. A polymerase chain reaction (PCR) or culture of preputial smegma for bovine genital 

campylobacteriosis (Campylobacter fetus ssp. venerealis) with negative results. The 

immunofluorescent antibody test may be used only as a screening test under the condition 

that samples that react positively must be retested using a suitable confirmatory test such as 

a PCR or culture of preputial smegma with negative results; 

 4.4. A PCR or a microscopic examination of a culture of preputial smegma for trichomoniasis 

(Trichomonas foetus) with negative results; 

 4.5. The donor bull was tested with an AGID of ELISA test for the bluetongue virus (BTV) group on 

blood serum performed priot to the first day of semen collection, at least every 60 days during 

the collection period, and between 21 and 60 days after semen collection, with negative 

results; 

 Or 4.5.1. The donor bull was tested with a whole blood PCR test for BTV group with one negative 

test at the beginning and conclusion of the collection period, and at least every 28 

days during the period of semen collection; 

 Or 4.5.2. The donor bull was tested with a whole-blood virus isolation test for BTV group with 

one negative test at the beginning and conclusion of the collection period, and at least 

every 7 days during the period of semen collection;

5. The semen was collected and processed under my supervision and placed in individual ampules or 

straws which were permanently marked with the name of the donor, his registration number or the 

collection code; 

6. Semen collection equipment which came into contact with bulls or their secretions and excretions 

was thoroughly disinfected after each use, and good laboratory practices were followed during 

collection and processing of semen in order to minimize the possible introduction of microbial 

contamination; 

7. Antibiotics were added to the semen and semen extender in amounts and combinations consistent 

with the standards described in “Certified Semen Services (CSS) Minimum Requirements for Disease 

Control of Semen Produced for AI,” Appendix I, website: 

http://www.naab-css.org/about_css/disease_control-2002.html; 

8. No biological products other than frozen semen or embryos qualified for shipment to the United 

States were present in the containers prior to use for export of semen to the USA; 

9. The storage and shipping containers are either new or cleaned; and 

10. Only virgin liquid nitrogen was used to export semen to the USA; 
certificaatnummer: 

 Page 3 of 4

Document ref. : NVWA_CLH_B_US_rundersperma_EN-certificaatmodel 2.4.rtf 

11. For sexed semen: 

 The semen collected and processed under my supervision was shipped to the semen sexing facility 

within the Member State of collection under seal or was maintained under the oversight of a center 

or official veterinarian. 

Gedaan te / Done at / Ausgefertigt in / Fait à / Hecho en 

Op / On / Am / Le / El 

Name and qualification of the centre veterinarian 

Signature and stamp of the centre veterinarian 

Section B ( to be signed by the Official Veterinarian after the Centre Veterinarian has signed) 

I, the undersigned Official Veterinarian of The Netherlands certify that: 

1. The Member State where the semen was collected is considered by the USDA to be free of foot-andmouth disease and rinderpest, as listed in 9 CFR Part 94 and other official publications, and was free

of these diseases at the time of semen collection; 

2. The Member State where the semen was collected is free of contagious bovine pleuropneumonia; 

3. The donor animals for the semen to be exported to the United States have been part of the national 

herd of the Member State where the semen was collected for a minimum of 60 days and are free 

from any movement or quarantine restrictions; 

4. The semen collection centre, hereinafter “SCC,” was approved by the competent authority of the 

Member State where the semen was collected; 

5. Health tests required for export to the United States of bovine semen were performed by testing 

methods recognized by the Office International des Epizooties (OIE) Manual of Diagnostic Tests and 

Vaccines for Terrestrial Animals, as acceptable for international trade; 

6. The laboratory tests mentioned in IV. 4.2. to IV. 4.5.2. were carried out with negative results in a 

laboratory approved by the competent veterinary services; 

7. Ruminant products used in commercial semen extenders in the Member State where the semen was 

collected were sourced from countries considered by USDA to be free from foot-and-mouth disease 

and rinderpest as listed in 9 CFR Part 94 and other official publications; 

8. The semen to be exported to the United States was maintained under lock and key or in the custody 

of the SCC veterinarian, and segregated from other semen of lesser health status, until it was placed 

in the shipping container and sealed with official seals of the Member State where the semen was 

collected; 

9. None of the semen for export to the USA has been stored or transported in containers with semen 

produced under less than equivalent animal health conditions; 

10. The integrity of the total shipment and continuity of storage conditions for semen produced in 

different approved SCC units and collected in the Member State listed on this health certificate (*); 

11. The shipping containers were sealed with an approved seal from the competent authority of the 

exporting Member State, and the seal number is recorded on the health certificate; 

12. The semen is routed directly to the United States from the Member State in which it was collected 

with no stops en route other than those provided on the USDA import permit; and 

13. The Centre Veterinarian that completed Section A of this certificate is authorized by the National

Veterinary Service to perform this service. 

14. For sexed semen: 

 14.1. The semen sexing laboratory used to sex the semen for export to the United States is 

located in the EU Member State where the semen was collected or was imported from the 

United States meeting all EU import requirements. The semen collection center is under the 

supervision of an approved Center Veterinarian, and is regularly inspected and approved in 

accordance with EU Directive 88/407/EEC. The sexing facility followed the United States 

Department of Agriculture approved “Cleaning and Disinfection Standard Operating Protocol” 

while processing this semen for export to the United States; 

 14.2. The integrity of this shipment was maintained through the semen sexing process and no 

semen from other donors was mixed with semen that originated from the animals listed in 

Part I. 

15. All tests were carried out in a laboratory approved by the national competent authority. 

(*)Delete as appropriate. 
certificaatnummer: 

 Page 4 of 4

Document ref. : NVWA_CLH_B_US_rundersperma_EN-certificaatmodel 2.4.rtf 

Gedaan te / Done at / Ausgefertigt in / Fait à / Hecho en 

Op / On / Am / Le / El 

Handtekening officiële dierenarts / Signature of the official veterinarian / 

Unterschrift des amtlichen Tierarztes / Signature du vétérinaire officiel / 

Firma veterinario oficial 

Naam in hoofdletters / Name in capital letters / Name in Grossbuchstaben / 

Nom en lettres capitales / Nombre en letras capitales