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ASF NVSL-FM-1094.05 FM-FADDL0052

ASF PCR Control QC Summary 

Sheet 

ASF NVSL-FM-1129.06 FM-FADDL0087

African Swine Fever rPCR Assay on 

the Applied Biosystems 7500 Realtime PCR System Worksheet

ASF NVSL-FM-1134.03 FM-FADDL0092

African Swine Fever rPCR Assay on 

the Applied BiosystemsTM 

QuantStudioTM 5 Real-Time PCR 

System Worksheet

ASF NVSL-SOP-0591.04 SOP-FADDL0067

Extraction of Total DNA from 

Tissues, Tissue Culture Supernatant, 

and Whole Blood Using a Qiagen 

DNeasy® Blood and Tissue Mini Kit 

ASF NVSL-SOP-0646.05 SOP-FADDL0072

Preparation, Performance and 

Interpretation of the African Swine 

Fever rPCR Assay on the Applied 

Biosystems 7500 Real-time PCR 

System

ASF NVSL-SOP-0650.05 SOP-PS-0002

Preparation, Performance, and 

Interpretation of the African Swine 

Fever rPCR Assay on the Applied 

Biosystems® QuantStudio 5 Realtime PCR System

ASF, CSF NVSL-FM-2124.01 N/A

African Swine Fever and Classical 

Swine Fever Real-Time RT-PCR 

Assay on the Applied Biosystems TM 

QuantStudio 5 TM and the Applied 

Biosystems TM 7500 Real-Time PCR 

Systems 

ASF, CSF NVSL-MAN-0100.02 MAN-NAHLN0006

African Swine Fever (ASF)/Classical 

Swine Fever (CSF) Surveillance - 

NAHLN Laboratory Manual

ASF, CSF NVSL-SOP-1201.02 N/A

Preparation, Performance, and 

Interpretation of the African and 

Classical Swine Fever Real-Time 

Reverse-Transcription Multiplex 

Polymerase Chain Reaction (rRTPCR) on the Applied BiosystemsTM 

QuantStudioTM 5 Real-Time System 

ASF, CSF NVSL-SOP-1203.02 WI-AV-0035

Preparation, Performance, and 

Interpretation of the African and 

Classical Swine Fever Real-Time 

Reverse-Transcription Multiplex 

Polymerase Chain Reaction (rRTPCR) on the Applied BiosystemsTM 

7500 Real-Time System

ASF, CSF, 

FMD NVSL-FM-1080.05 FM-FADDL0038

Recovery of Sample from a Swab 

Worksheet

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ASF, CSF, 

FMD NVSL-FM-1081.05 FM-FADDL0039

Recovery of Sample from Tissue 

Worksheet 

ASF, CSF, 

FMD NVSL-FM-1127.04 FM-FADDL0085

Nucleic Acid Extraction Using the 

MagMAXTM CORE Nucleic Acid 

Purification Kit on a Magnetic Particle 

Processor Worksheet

ASF, CSF, 

FMD NVSL-FM-1128.06 FM-FADDL0086

Nucleic Acid Extraction Using the 

MagMAXTM Pathogen RNA/ DNA Kit 

on a Magnetic Particle Processor 

Worksheet

ASF, CSF, 

FMD NVSL-SOP-0589.03 SOP-FADDL0016

Preparation of Tissues, Tissue 

Homogenates, and Swabs for 

Nucleic Acid Extraction 

ASF, CSF, 

FMD NVSL-SOP-0643.04 SOP-FADDL0069

Nucleic Acid Extraction Using the 

MagMAXTM CORE Nucleic Acid 

Purification Kit on a Magnetic Particle 

Processor

ASF, CSF, 

FMD NVSL-SOP-0644.04 SOP-FADDL0070

Nucleic Acid Extraction using the 

MagMAX Pathogen RNA/DNA kit on 

a Magnetic Particle Processor

ASF, CSF, 

FMD NVSL-SOP-0645.02 SOP-FADDL0071

Nucleic Acid Extraction Using the 

INDICAL BIOSCIENCE IndiMag 

Pathogen Kit on a Magnetic Particle 

Processor

ASF, CSF, 

FMD NVSL-SOP-1059.05 N/A

Production and Use of a Reference 

Panel for Quality Control of Primers 

and Probes for PCR 

ASF, CSF, 

FMD NVSL-WI-1174.03 WI-RVS-0003

Work Instructions for Use of a 

Reference Panel for QA/QC Testing 

of Primers and Probes for Real-time 

PCR Applications

ASF, FMD NVSL-FM-1083.04 FM-FADDL0041

Extraction of Total DNA from 

Tissues, Tissue Culture Supernatant, 

and Whole Blood Using a Qiagen 

DNeasy® Blood and Tissue Mini Kit 

Worksheet 

BSE NVSL-SOP-0886.02 SOP-PS-0048 Redirection of Bovine Spongiform 

Encephalopathy Samples 

BSE NVSL-SOP-0924.02 SOP-PS-0051

Quality Assurance: Proficiency 

Testing and Quality Monitoring of 

BSE ELISA Contract Laboratories by 

NVSL 

BSE NVSL-SOP-0927.02 SOP-RVS-0028

Protocol for Bovine Spongiform 

Encephalopathy (BSE) Approved 

National Animal Health Laboratory 

Network (NAHLN) Laboratories to 

Receive and Test Bovine Brain 

Samples and Report Results for BSE 

Surveillance 

CSF NVSL-FM-1093.05 FM-FADDL0051

CSF PCR Control QC Summary 

Sheet 

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CSF, FMD NVSL-FM-1126.04 NVSL-FM-1126

Classical Swine Fever and Foot and 

Mouth Disease Real-Time RT-PCR 

Assay Using the TaqMan® Fast 

Virus 1-Step Master Mix on the 

Applied Biosystems Quant Studio 5 

Real-time PCR Platform Worksheet

CSF, FMD NVSL-FM-1130.07 FM-FADDL0088

Classical Swine Fever and Foot and 

Mouth Disease rRT-PCR Using the 

TaqMan® Fast Virus 1-Step Master 

Mix on the Applied Biosystems 7500 

Real-time PCR Platform Worksheet

CSF, FMD NVSL-FM-1131.03 FM-FADDL0089

Nucleic Acid Extraction Using the 

INDICAL BIOSCIENCE IndiMag 

Pathogen Kit on a Magnetic Particle 

Processor Worksheet

CSF, FMD NVSL-SOP-0642.07 SOP-FADDL0068

Classical Swine Fever and Foot and 

Mouth Disease rRT-PCR Using the 

TaqMan® Fast Virus 1-Step Master 

Mix on the Applied Biosystems 7500 

Real-time PCR Platform

CSF, FMD NVSL-SOP-0647.03 SOP-FADDL0073

Classical Swine Fever and Foot and 

Mouth Disease rRT-PCR Using the 

TaqMan® Fast Virus 1-Step Master 

Mix on the Applied Biosystems 

QuantStudio 5 Real-time PCR 

Platform

CWD NVSL-SOP-0883.02 SOP-PS-0010

Chronic Wasting Disease (CWD) 

Enzyme-Linked Immunosorbent 

Assay (ELISA) 

CWD 

ELISA, BSE 

ELISA

NVSL-FM-1583.05 NVSL-FM-1583 Information on "Initial Reactives” for 

TSE ELISA 

CWD IHC / 

Scrapie IHC NVSL-SOP-0878.03 SOP-PS-0007

Detection of Scrapie and Chronic 

Wasting Disease by 

Immunohistochemistry (IHC)

Fin Fish – VI 

(VHSV & 

SVCV)

NVSL-FM-0393.03 FM-BPA-0005 24 Well Plate Virus Isolation 

Worksheet 

Fin Fish – VI 

(VHSV & 

SVCV)

NVSL-FM-0408.04 FM-BPA-0020 Virus Isolation Worksheet 

Fin Fish – VI 

(VHSV & 

SVCV), 

VHS-PCR, 

ISA-PCR

NVSL-SOP-0285.05 SOP-BPA-0018 Isolation of Viral Agents from Finfish 

in Tissue Cell Culture 

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Fin Fish – VI 

(VHSV & 

SVCV), 

VHS-PCR, 

ISA-PCR

NVSL-SOP-0324.03 SOP-BPA-0054 Disruption of Fish Tissue by MicroBead Beating 

FMD NVSL-FM-1092.04 FM-FADDL0050

FMD PCR Control QC Summary 

Sheet 

FMD/SVA 

Multiplex NVSL-FM-1132.03 FM-FADDL0090

Foot and Mouth Disease Virus and 

Senecavirus A Multiplex rRT-PCR 

Using the TaqMan® Fast Virus 1-

Step Master Mix on the Applied 

Biosystems 7500 Real-time PCR 

Platform Worksheet

FMD/SVA 

Multiplex NVSL-SOP-0648.02 SOP-FADDL0075

Preparation, Performance and 

Interpretation of the Foot and Mouth 

Disease Virus and Senecavirus A 

Multiplex rRT-PCR Assay on the 

Applied Biosystems 7500 FAST Realtime PCR System

IAV-A NVSL-SOP-0018.05 SOP-AV-0068

Isolation of Avian Influenza and Avian 

Paramyxoviruses Viruses in Chicken 

Embryos from Avian Species

IAV-A NVSL-WI-0042.05 WI-AV-0050

Quick Reference for Real-Time RTPCR Influenza A Detection using the 

IAV M 2009 Assay (for AB7500 Fast 

and Cepheid SmartCycler II) 

IAV-A NVSL-WI-0048.06 WI-AV-0051 NVSL Testing Guidance for Post 

C&D Environmental Samples 

IAV-A NVSL-WI-0053.02 WI-AV-0052

Quick Reference for Real-Time RTPCR Influenza A Assays for Subtype 

H5 or H7 Detection (for AB7500 Fast 

and Cepheid Smart Cycler) 

IAV-A, IAVSNVSL-Process0002.02Process-DVL0001Influenza A Test Comparison for 

Avian and Swine

IAV-A, IAVS, ND NVSL-WI-0054.04 WI-BPA-0032

Evaluation and Quality Control 

Testing of Real Time PCR Primers 

and Probes

IAV-A, IAVS, ND NVSL-WI-0055.02 WI-BPA-0048 RNA Extraction Using a Magnetic 

Particle Processor 

IAV-A, ND NVSL-FM-0039.04 FM-AV-0038 RRT-PCR Primer and Probe Quality 

Control Worksheet 

IAV-A, ND NVSL-Process0001.02

Process-AV0001Testing and Confirmation for Avian 

influenza and Newcastle Disease

IAV-A, ND NVSL-SOP-0068.05 SOP-BPA-0010

Real-time RT-PCR Detection of 

Influenza A and Avian Paramyxovirus 

Type-1

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IAV-A, ND NVSL-WI-0038.06 WI-AV-0037

Programming and Analysis for the 

Cepheid SmartCycler Real-Time 

Instrument

IAV-A, ND NVSL-WI-0039.05 WI-AV-0038

Programming and Analysis for the 

Roche LightCycler® 480 Real-Time 

Instrument 

IAV-A, ND NVSL-WI-0040.04 WI-AV-0039

Programming and Analysis for the 

Applied Biosystems 7900 Real-Time 

Instrument 

IAV-S NVSL-SOP-0289.03 SOP-BPA-0019

Real-Time RT-PCR for the Detection 

of Influenza A Virus in Swine (IAV-S) 

in Clinical Samples 

IAV-S NVSL-SOP-0290.03 SOP-BPA-0023

Amplification and Purification of PCR 

Products for Direct Sequencing of 

Hemagglutinin, Neuraminidase, and 

Matrix Genes of Swine Influenza 

Virus 

IAV-S NVSL-SOP-0294.02 SOP-BPA-0044 Swine Influenza Virus Isolation 

IAV-S NVSL-SOP-0315.04 SOP-BPA-0052

Real-Time RT-PCR for Subtyping 

Type A Swine Influenza Virus in 

Clinical Samples 

IAV-S NVSL-WI-0209.03 WI-NAHLN0011

Minimum Requirements for 

Submitting NAHLN Swine Influenza 

Virus Sequencing to GenBank 

ISA-PCR NVSL-FM-0479.05 FM-BPA-0091 ISAV RT-PCR 

ISA-PCR NVSL-FM-0481.03 FM-BPA-0093 ISAV VetMAX plus - Multiplex One 

Step RT-PCR 

ISA-PCR NVSL-SOP-0323.02 SOP-BPA-0053

Dissection of Fish for Detection of 

Viruses During Large-Scale, 

Targeted Surveillance for ISAV 

ISA-PCR NVSL-SOP-0326.03 SOP-BPA-0056 Infectious Salmon Anemia Real-time 

RT-PCR - segment 8 Snow primers 

ISA-PCR NVSL-SOP-0327.03 SOP-BPA-0060 Infectious Salmon Anemia RT-PCR - 

Segment 6 and 8 

ISA-PCR, 

VHS-PCR NVSL-FM-0480.03 FM-BPA-0092 KingFisher AMB1836 Extraction Worksheet for Aquaculture 

N/A NVSL-FM-1347.04 FM-NAHLN0051

NAHLN Quality Standard 

Harmonization Checklist

N/A NVSL-MAN-0028.04 MAN-NAHLN0002

NAHLN Laboratory Guide for Foreign 

Animal Disease (FAD) Investigations

N/A NVSL-Policy-0036.02 Policy-NAHLN0006 NAHLN Laboratory Performance 

N/A NVSL-Policy-0037.03 Policy-NAHLN0007

NAHLN Quality Standard - 

Harmonization of AAVLD and 

ISO/IEC 17025:2017

N/A NVSL-WI-0702.05 WI-NAHLN0038

NAHLN Laboratory Controlled Copy 

SOP Responsibilities 

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N/A NVSL-WI-0721.04 WI-RVS-0003

Registering for a Proficiency Test 

(PT) Event in the APHIS Laboratory 

Portal

N/A NVSL-WI-0729.10 WI-NAHLN0038NAHLN Test Reference Chart

N/A NVSL-WI-1781.01 N/A NAHLN Electronic Messaging Guide

ND NVSL-WI-0041.04 WI-AV-0045

Quick Reference for Real-Time RTPCR Avian Paramyxovirus Type-1 

Detection (for AB7500 Fast and 

Cepheid SmartCycler) 

VHS-PCR NVSL-FM-0482.04 FM-BPA-0094 VHSV rRT-PCR Worksheet 

VHS-PCR NVSL-SOP-0331.02 SOP-BPA-0063 Viral Hemorrhagic Septicemia Virus 

Real-time RT-PCR 

VHS-PCR, 

ISA-PCR NVSL-FM-0458.04 FM-BPA-0070 MagMAX AM1836 Extraction Worksheet for Aquaculture 

VHS-PCR, 

ISA-PCR NVSL-SOP-0325.04 SOP-BPA-0055 Extraction of Nucleic Acid (DNA and RNA) from Fish Tissues - AM1836 

VSV NVSL-FM-0405.05 NVSL-FM-0405 Vesicular Stomatitis Complement 

Fixation Bench Record 

VSV NVSL-FM-0406.04 FM-BPA-0018

Complement Fixation Assay for 

Vesicular Stomatitis Complement 

Titration Worksheet 

VSV NVSL-FM-0407.04 FM-BPA-0019

Complement Fixation Assay for 

Vesicular Stomatitis

Hemolysin Titration Worksheet 

VSV NVSL-FM-0420.04 FM-BPA-0032 Washing & Standardization of Sheep 

Red Blood Cells 

VSV NVSL-FM-0483.04 FM-BPA-0095 Vesicular Stomatitis PCR Worksheet 

Using Fast Virus 1-Step Kit 

VSV NVSL-FM-0485.04 FM-BPA-0097 MagMAX Express 96 Extraction 

Worksheet (200μl sample) 

VSV NVSL-SOP-0281.03 SOP-BPA-0011

Complement Fixation Test for 

Detection of Antibodies to Vesicular 

Stomatitis Virus 

VSV NVSL-SOP-0282.03 SOP-BPA-0014

Reagent Preparation and Evaluation 

for the Vesicular Stomatitis 

Complement Fixation Assay 

VSV NVSL-SOP-0334.04 SOP-FADDL0014

Real-time Reverse-Transcriptase 

Polymerase Chain Reaction for the 

Detection of Vesiculovirus Nucleic 

Acids Using the ABI 7500 Detection 

System

VSV NVSL-WI-0225.02 WI-NAHLN0030

RNA Extraction of Vesicular 

Stomatitis Using MagMAX Bead Kits 

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Revision Document Title

NVSL-SOP-0014 .07 Intravenous Pathogenicity Assessments for Avian Influenza Virus in 

Chickens

NVSL-SOP-0018 .05 Isolation of Avian Influenza and Avian Paramyxoviruses Viruses in 

Chicken Empryos from Avian Species

NVSL-SOP-0066 .01 Agar Gel Immunodiffusion Test to Detect Antibodies to Avian 

Encephalomyelitis Virus

NVSL-SOP-0068 .05 Real-time RT-PCR Detection of Influenza A and Avian 

Paramyxovirus Type-1 

NVSL-SOP-0074 .02 Avian Influenza Real-Time RT-PCR Testing for Qualified NPIP 

Primary Breeder Authorized Laboratories

NVSL-SOP-0076 .05 Quality Control Testing of Taylorella spp. Media

NVSL-SOP-0082 .05 Pulsed Field Gel Electrophoresis (PFGE) on Taylorella isolates

NVSL-SOP-0089 .05 Salmonella pullorum Microagglutination Test

NVSL-SOP-0091 .04 Salmonella pullorum Tube Agglutination Test

NVSL-SOP-0180 .07 Taylorella spp. Isolation and Identification

NVSL-SOP-0181 .05 Taylorella spp. Direct Fluorescent Antibody Test

NVSL-SOP-0183 .05 Real-time Polymerase Chain Reaction Test for Rapid Identification 

and Speciation of Taylorella spp.

NVSL-SOP-0281 .03 Complement Fixation Test for Detection of Antibodies to Vesicular 

Stomatitis Virus

NVSL-SOP-0292 .01 Virus Isolation Procedure for Pseudorabies Virus (PRV)

NVSL-SOP-0306 .06 Virus Neutralization Test for Detection of Antibodies Against 

Pseudorabies Virus

NVSL-SOP-0310 .05 Serology Testing for Pseudorabies in the Diagnostic Virology 

Laboratory

NVSL-SOP-0323 .02 Dissection of Fish for Detection of Viruses During Large-Scale, 

Targeted Surveillance for ISAV

NVSL-SOP-0324 .03 Disruption of Fish Tissue by Micro-Bead Beating

NVSL-SOP-0325 .04 Extraction of Nucleic Acid (DNA and RNA) from Fish Tissues - 

AM1836

NVSL-SOP-0326 .03 Infectious Salmon Anemia Real-time RT-PCR - segment 8 Snow 

primers

NVSL-SOP-0327 .03 Infectious Salmon Anemia RT-PCR - Segment 6 and 8

NVSL-SOP-0331 .02 Viral Hemorrhagic Septicemia Virus Real-time RT-PCR

NVSL-SOP-0533 .03 Serotype Identification of Orbiviruses (BTV, EHDV, and AHSV) by 

RT-PCR

NVSL-SOP-0536 .03 Isolation of Bluetongue Virus

NVSL-SOP-0538 .02 Equine Arteritis Virus Isolation

NVSL-SOP-0541 .02 General Protocol for Isolation of Equine Viruses

NVSL-SOP-0558 .02 Microtiter Virus Neutralization Test for Equine Viral Arteritis

NVSL-SOP-0562 .03

Requirements for Equine Infectious Anemia Agar Gel 

Immunodiffusion (Coggins) and Enzyme-Linked Immunosorbent 

Assay Testing at APHIS-Approved Laboratories

NVSL-SOP-0564 .02 Virus Neutralization Test for Bluetongue and Epizootic Hemorrhagic 

Disease Viruses

NVSL-SOP-0569 .06 Real-Time RT-PCR Assays for Detection of Bluetongue, Epizootic 

Hemorrhagic Disease and African Horse Sickness Viruses

NVSL-SOP-0571 .03 Procedure for Performing Miscellaneous Gel-based PCR Assays: 

EAV; Bunyavirus; and EHV-2, -3, & -5

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NVSL-SOP-0591 .04

Extraction of Total DNA from Tissues, Tissue Culture Supernatant, 

and Whole Blood Using a Qiagen DNeasy® Blood and Tissue Mini 

Kit

NVSL-SOP-0616 .01 Nucleic Acid Sequencing of the Foot and Mouth Disease S 

Fragment

NVSL-SOP-0617 .02 Nucleic Acid Sequencing of the Foot and Mouth Disease L Fragment

NVSL-SOP-0618 .01 Nucleic Acid Sequencing of the Foot and Mouth Disease P1 Region

NVSL-SOP-0619 .01 Nucleic Acid Sequencing of the Foot and Mouth Disease 3’ Region

NVSL-SOP-0642 .07

Classical Swine Fever and Foot and Mouth Disease rRT-PCR Using 

the TaqMan® Fast Virus 1-Step Master Mix on the Applied 

Biosystems 7500 Real-time PCR Platform

NVSL-SOP-0644 .04 Nucleic Acid Extraction using the MagMAX Pathogen RNA/DNA Kit 

on a Magnetic Particle Processor

NVSL-SOP-0659 .03 Testing Requirements for Culture Media; Brucella spp. Isolation

NVSL-SOP-0660 .03 Isolation: Brucella spp.

NVSL-SOP-0662 .03 Evaluation of Individual Lots of Brucella Ring Test Antigen

NVSL-SOP-0664 .03 Procedure for the Propagation of Brucellaphages

NVSL-SOP-0668 .03 AMOS PCR

NVSL-SOP-0670 .04 Identification of Brucella species

NVSL-SOP-0682 .04 16s rDNA, rpoB, hsp65, ITS Sequencing for Bacterial Species and 

Prp Sequencing for Scrapie susceptibility genotyping.

NVSL-SOP-0690 .06 VersaTrekTM Liquid Culture of Mycobacterium species 

NVSL-SOP-0692 .06 HPC Decontamination of Tissues Submitted for Mycobacteria 

Culture

NVSL-SOP-0693 .04 HPC Decontamination of Fecal Swabs and Feces Submitted for 

Mycobacteria Culture

NVSL-SOP-0695 .02 Procedures for Brucella Milk Ring Tests

NVSL-SOP-0696 .07 NaOH Decontamination Method for Mycobacterial Culture

NVSL-SOP-0697 .02

Direct PCR Assay from Feces for Detection of Mycobacterium avium 

subsp. paratuberculosis Using Magmax Core Mechanical Lysis and 

Purification Kit

NVSL-SOP-0701 .07 Inactivation of Brucella

NVSL-SOP-0708 .02 Isolation of Mycobacteria Using Solid Media

NVSL-SOP-0710 .05 MGITTM 960 Liquid Culture of Mycobacteria species

NVSL-SOP-0878 .03 Detection of Scrapie and Chronic Wasting Disease by 

Immunohistochemistry (IHC)

NVSL-SOP-0882 .03 Scrapie Enzyme-Linked Immunosorbent Assay (ELISA)

NVSL-SOP-0927 .02

Protocol for Bovine Spongiform Encephalopathy (BSE) Approved 

National Animal Health Laboratory Network (NAHLN) Laboratories to 

Receive and Test Bovine Brain Samples and Report Results for 

BSE Surveillance

NVSL-SOP-0930 .04 Detection of Mycobacterial Nucleic Acid in Formalin-Fixed ParaffinEmbedded Tissues by Polymerase Chain Reaction 

NVSL-SOP-0963 .01 Preparation of Antiserum for the Avian Influenza Agar Gel 

Immunodiffusion Test

NVSL-SOP-0965 .02 Preparation of Optimal Dilutions for Antigen and Antiserum for the 

Avian Influenza Agar Gel Immunodiffusion Test

NVSL-SOP-1033 .08 Standard Operating Procedure (SOP) for Cell Culture Media 

Preparation

NVSL-SOP-1035 .07 Standard Operating Procedure (SOP) for Production of Virus Stocks 

Used in the Various Virus Neutralization (VN) Tests 

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NVSL-SOP-1047 .02

Standard Operating Procedure (SOP) for Quality Control Testing of 

Virus Stocks Prepared by the Reagents and Vaccine Services 

Section

NVSL-SOP-1050 .05 Classical Swine Fever 96 Well Plate Preparation for the 

Immunoperoxidase Test

NVSL-SOP-1051 .06 Enzyme Linked Immunosorbent Assay (ELISA) for Titrating Rabbit 

and Guinea Pig Antisera Against Vesicular Disease Agents

NVSL-SOP-1052 .05 Enzyme Linked Immunosorbent Assay (ELISA) for Testing 

Specificity of Rabbit Antisera Against Vesicular Disease Agents

NVSL-SOP-1053 .05 Enzyme Linked Immunosorbent Assay (ELISA) for Testing 

Specificity of Guinea Pig Antisera Against Vesicular Disease Agents

NVSL-SOP-1062 .07 Standard operating Procedure for Foot-and Mouth Disease (FMD) 

RT-qPCR Proficiency Panel Assembly and Distribution 

NVSL-SOP-1069 .06 Standard operating Procedure for Classical Swine Fever (CSF) rRTPCR Proficiency Panel Assembly and Distribution

NVSL-SOP-1071 .06

Standard Operating Procedure (SOP) of Demonstration of 

Contagious Bovine Pleuropneumonia in Foreign Animal Disease 

Diagnostician Course

NVSL-SOP-1077 .03

Standard Operating Procedure for the Application and Authorization 

for the Use of Biological Materials Containing Rinderpest Virus 

(RPV)

NVSL-SOP-1078 .02

Handling, packaging, transfer and shipping of Rinderpest Virus 

(RPV) containing Materials from the Reagents and Vaccine Services 

Section (RVSS) RPV holding Facility.

NVSL-SOP-1080 .02

General Procedures for Manipulating Rinderpest virus (RPV)-

Containing Materials in the Reagents and Vaccines Service Section 

(RVSS) of the Foreign Animal Disease Laboratory (FADDL)

NVSL-SOP-1084 .03 Workflow for Receipt and Transfer of DNA Materials for NaOH 

Safety Treatment

NVSL-SOP-1085 .06 Disinfectants and Cleaners Used in the Reagents and Vaccines 

Services Section (RVSS)

NVSL-SOP-1087 .05 Standard Operating Procedure (SOP) for Preparation of Vesicular 

Exanthema of Swine Virus (VESV) Slides

NVSL-SOP-1092 .05

Production of Antisera against Antigens of Foot and Mouth Disease 

Virus (FMDV), Swine Vesicular Disease Virus (SVDV), and Vesicular 

Stomatitis Virus (VSV)

NVSL-SOP-1094 .05 Workflow for Receipt, Process and Transfer of DNA Materials for 

NaOH Safety Treatment using Safety Treatment Database 

NVSL-SOP-1095 .03 Solid Phase Competition ELISA for Detecting Antibodies against 

Foot-and-Mouth Disease Virus

NVSL-SOP-1101 .04

Production of Recombinant Foot-and-Mouth Disease Virus (FMDV) 

3D RNA Dependent RNA Polymerase for Virus Infection Associated 

Antigen (VIAA) Test

NVSL-SOP-1102 .03

Propagation of Foot and Mouth Disease Virus (FMDV), Swine 

Vesicular Disease Virus (SVDV) and Vesicular Stomatitis Virus 

(VSV) in Roller Bottles for Antigen Purification

NVSL-SOP-1106 .03 Standard Operating Procedure (SOP) for Preparation of African 

Swine Fever Virus (ASFV) Slides

NVSL-SOP-1107 .03 Production of Binary Ethyleneimine (BEI) Inactivated Positive 

Amplification Controls (PAC) and Positive Extraction Controls (PEC)

NVSL-SOP-1108 .03 Purification of Vesicular Stomatitis Virus (VSV) Antigen

NVSL-SOP-1109 .05 Inactivation of African Swine Fever Virus (ASFV) in Tissue Culture 

Supernatant using 3mM Binary Ethylenimine (BEI)

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NVSL-SOP-1111 .03

Preparation, Performance, and Interpretation of a Real-Time PCR 

Assay for the Detection of Capripoxviruses on ABI 7500 Fast 

Thermocycler Using TaqMan Fast Virus One-step Master Mix

NVSL-SOP-1116 .03 Complement Fixation Test for Detection of Antibodies to Babesia 

caballi and Theileria (Babesia) equi—Microtitration Test

NVSL-SOP-1117 .07 Indirect Fluorescent Antibody Test for Detection of Antibodies to 

Babesia caballi and Theileria (Babesia) equi

NVSL-SOP-1123 .02 Complement Fixation Test for Detection of Antibodies to Anaplasma 

marginale—Microtitration Test

NVSL-SOP-1125 .03 Johne's Complement Fixation Test

NVSL-SOP-1128 .03 Complement Fixation Test for Detection of Antibodies to Brucella 

abortus and Brucella suis

NVSL-SOP-1129 .07 Plate Agglutination Test for Detection of Antibodies to Brucella 

abortus/suis

NVSL-SOP-1130 .05 Buffered Acidified Plate Antigen Test for Detection of Antibodies to 

Brucella 

NVSL-SOP-1132 .04 Tube Agglutination Test for Detection of Antibodies to Brucella 

abortus/suis

NVSL-SOP-1136 .05 Performance of ID Screen Brucellosis Serum Indirect Multi-species 

ELISA

NVSL-SOP-1140 .05 Card Test for Detection of Antibodies to Brucella

NVSL-SOP-1142 .05 Anaplasma Antibody Test Kit, cELISA v2

NVSL-SOP-1144 .11 Brucella Fluorescence Polarization Assay (FPA) Using Tubes

NVSL-SOP-1145 .04 VMRD cELISA Kits for Babesia caballi and Babesia equi

NVSL-SOP-1151 .06 IDEXX Johne's Disease ELISA Kit

NVSL-SOP-1152 .05 Contagious Equine Metritis Complement Fixation Test

NVSL-SOP-1154 .04 IDEXX ELISA Antibody Test Kits for Mycobacterium bovis

NVSL-SOP-1158 .04 Johne's Agar Gel Immunodiffusion Test

NVSL-SOP-1164 .05 Mycobacterium bovis Gamma Interferon Test Kit for Cattle 

NVSL-SOP-1193 .01 Preparation of African Swine Fever Virus (ASFV) Antigen Plates and 

Strips for Antibody Detection

NVSL-SOP-1201 .02

Preparation, Performance, and Interpretation of the African and 

Classical Swine Fever Real-Time Reverse-Transcription Multiplex 

Polymerase Chain Reaction (rRT-PCR) on the Applied BiosystemsTM 

QuantStudioTM 5 Real-Time System 

NVSL-SOP-1203 .02

Preparation, Performance, and Interpretation of the African and 

Classical Swine Fever Real-Time Reverse-Transcription Multiplex 

Polymerase Chain Reaction (rRT-PCR) on the Applied BiosystemsTM 

7500 Real-Time System

10/11/2023

Contact nvsl.mastercontrol@usda.gov for inquiries on 

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