Koninkrijk der Nederlanden 

 GEZONDHEIDSCERTIFICAAT 

 (veterinair certificaat) certificaatnummer: 

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Koninkrijk der Nederlanden Kingdom of the Netherlands Königreich der Niederlande Royaume des Pays-Bas Reino de los Países Bajos 

Ministerie van Economische 

Zaken 

Ministry of Economic Affairs Ministerium für Wirtschaft Ministère des Affaires 

économiques 

Ministerio de Economía 

Nederlandse Voedsel- en 

Warenautoriteit 

Netherlands Food and 

Consumer Product Safety 

Authority 

Niederländische Behörde für 

Ernährung und 

Produktsicherheit 

Autorité néerlandaise de 

surveillance des produits 

alimentaires et non 

alimentaires 

Autoridad holandesa de la 

seguridad Alimentaria y de los 

Productos de consumo 

Gezondheidscertificaat Health Certificate Gesundheitsbescheinigung Certificat Sanitaire Certificado Sanitario 

Document ref. : NVWA_CLH_B_US_runderembryo's_EN-certificaatmodel 2.6 

HEALTH CERTIFICATE FOR EXPORT OF IN VIVO DERIVED BOVINE EMBRYOS FROM FOOT-AND-MOUTH 

DISEASE-FREE MEMBER STATES OF THE EUROPEAN UNION TO THE UNITED STATES OF AMERICA 

I. IDENTIFICATION OF THE PRODUCTS 

Product no. Product Identification no. of dam Breed of dam

 

Product no. Identification no. of sire Breed of sire Member state of embryo collection

 

Batch no. Type of semen Packing (Number of straws) Date of embryo collection Collection code

 sexed / nonsexed(*)

Seal number :

II. ORIGIN OF THE PRODUCTS 

Product no. Approval no. embryo collection team Name and address embryo collection team

Name and address of exporter :

III. DESTINATION OF THE PRODUCTS

Means of conveyance :

Port of entry :

Name and address of consignee :

IV. HEALTH ATTESTATION 

Section A (to be signed by the Team Veterinarian)

I, the undersigned Team Veterinarian of the described embryo collection team, hereinafter “ECT,” certify, 

either by direct examination or based on supporting documentation in my possession that has been 

separately attested to by an official veterinarian, that:

1. During the 12 months prior to the collection of embryos for export to the United States, there was no 

clinical or pathological evidence of brucellosis or tuberculosis (TB) found in the donor dams or on any 

premises on which the donor dams were located during that time; 

2. During the 60 days prior to the collection of embryos for export to the United States, the donor dams 

were not corralled, pastured, or held with animals of lesser health status or under any restrictions 

which would make them ineligible as embryo donors for export to the United States 

3. During the 60 days prior to the collection of embryos for export to the United States, the donor dams 

were inspected at least once and appeared healthy and were found clinically free of contagious or 

communicable diseases; 

4. Each of the donors were examined on the day of embryo collection and appeared healthy and were 

clinically free of contagious or communicable diseases; 

5. The donor dams originated from herds officially free of tuberculosis; 

6. Either(*) The embryos were collected prior to June 1 2011; 

 Or(*) The embryos were collected on or after June 1 2011 from donors that were negative to 

two serum neutralization tests (VNT) for Schmallenberg virus (using a 1 : 8 cutoff titer), 

with the first performed within 30 days prior to collection, and the second between 28 and 

60 days after collection. Tests were performed in a laboratory approved by the national 

Competent Authority ; 
certificaatnummer: 

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Document ref. : NVWA_CLH_B_US_runderembryo's_EN-certificaatmodel 2.6 

7. 7.1. The semen used to fertilize the embryos for export to the United States was collected in an 

approved semen collection centre (SCC), in accordance with legislation in force, notably 

Council Directive 88/407/EEC, as amended. At the time of collection of the semen, the 

Member State in which the semen was collected was considered by the USDA to be free of 

foot-and-mouth disease and rinderpest, as listed in Title 9 Code of Federal Regulations, 

Part 94 and other official publications. In addition 

 Either(*) The semen was collected prior to June 1 2011; 

 Or(*) The semen was collected on or after June 1, 2011 from donors that were negative to two 

serum neutralization tests (VNT) for Schmallenberg virus (using a 1 : 8 cutoff titer), with 

the first performed within 30 days prior to collection, and the second between 28 and 60 

days after collection; 

 Tests were performed in a laboratory approved by the national Competent Authority. 

 7.2.(*) If embryos were fertilized with sexed semen: 

 7.2.1. The semen sexing facility used to sex the semen for export to the United States is 

located in the EU Member State where the semen was collected. The semen 

collection center is under the supervision of an approved Center Veterinarian, and is 

regularly inspected and approved in accordance with EU Directive 88/407/EEC. The 

sexing facility followed the United States Department of Agriculture approved 

“Cleaning and Disinfection Standard Operating Protocol” while processing this 

semen; 

 7.2.2. The integrity of the semen shipment was maintained through the semen sexing 

process and no semen from other donors was mixed with semen during processing;

8. The embryos were collected using a closed collection system, and any instrument coming in contact 

with reproductive tract tissue or fluids was either new or equipment sterilized before use; 

9. The embryos were washed at least 10 times and treated with trypsin in accordance with the latest 

edition of the Manual of the International Embryo Transfer Society. After the last wash, each embryo 

was examined microscopically over its entire surface at not less than 50x magnification. The zona 

pellucida of each embryo was found to be intact and free from any adherent material subsequent to 

washing; 

10. Embryos from different donors were not washed together; 

11. The storage and shipping containers were clean, recently disinfected, and empty prior to use for this 

project, and only fresh liquid nitrogen has been used. 

(*)Delete as appropriate.

Gedaan te / Done at / Ausgefertigt in / Fait à / Hecho en 

Op / On / Am / Le / El 

Name and qualification of the team veterinarian 

Signature and stamp of the team veterinarian 

Section B ( to be signed by the Official Veterinarian after the Centre Veterinarian has signed) 

I, the undersigned Official Veterinarian of the Netherlands certify that:

1. The Member State in which the embryos were collected is considered by the USDA to be free from 

foot-and-mouth disease (FMD) and rinderpest as listed in Title 9 Code of Federal Regulations, Part 94 

and other official publications, and was free of these diseases at the time of embryo collection; 

2. The Member State in which the embryos were collected is free from contagious bovine 

pleuropneumonia; 

3. The donor dams were part of the national herd of the Member State in which the embryos were 

collected for a minimum of 60 days prior to collection and were free from any movement or quarantine 

restrictions; 

4. The embryos were collected from live cattle of documented health history and processed in 

accordance with the standards of the International Embryo Transfer Society (IETS) by an embryo 

collection team approved by the competent authority of the Member State in accordance with EU 

legislation in force, notably Council Directive 89/556/EEC, as amended; 

5. All diagnostic testing of the donor dams and sires were conducted in laboratories approved by the 

National Veterinary Services to conduct such tests for export; 

6. All media additives of animal origin were sourced from countries considered by the USDA to be free 

from FMD and rinderpest. Trypsin of porcine origin was sourced from countries considered by USDA to 

be free from FMD, rinderpest, classical swine fever and African swine fever as listed in 9 CFR Part 94

and other official publications; 

(https://www.aphis.usda.gov/wps/portal/aphis/ourfocus/importexport?1dmy&urile=wcm%3apath%3a
certificaatnummer: 

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Document ref. : NVWA_CLH_B_US_runderembryo's_EN-certificaatmodel 2.6 

%2Faphis_content_library%2Fsa_our_focus%2Fsa_animal_health%2Fsa_import_into_us%2Fct_anima

l_disease_status ) 

7. The embryos were maintained under lock and key or in the custody of the embryo collection team 

veterinarian until being sealed for direct transport to the United States; 

8. The Team Veterinarian that completed Section A of this certificate is authorized by the National 

Veterinary Service to perform this service. 

Notes: 

- A separate certificate must be issued for each consignment of embryos. 

- The original of this certificate must accompany the shipment. 

Gedaan te / Done at / Ausgefertigt in / Fait à / Hecho en 

Op / On / Am / Le / El 

Handtekening officiële dierenarts / Signature of the official veterinarian / 

Unterschrift des amtlichen Tierarztes / Signature du vétérinaire officiel / 

Firma veterinario oficial 

Naam in hoofdletters / Name in capital letters / Name in Grossbuchstaben / 

Nom en lettres capitales / Nombre en letras capitales