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Supplemental Assay Methods (SAMs)

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100 Series: Large Animal Virus Techniques

ID Title Date

101 Titration of Bovine Viral Diarrhea Virus in Vaccines --

102 Titration of Parainfluenza-3 Virus in Vaccines --

104

(146.07

KB)

Isolation of Parainfluenza-3 Virus from Nasal Excretions October 10,

2014

105 Titration of Bovine Rhinotracheitis Virus in Vaccines --

106

(181.85

KB)

Titration of Neutralizing Antibody (Constant Virus - Varying

Serum Method)

October 16,

2014

107

(164.92

KB)

Titration of Neutralizing Antibody (Constant Serum -

Varying Virus Method)

October 16,

2014

108

(189.22

KB)

Detection of Extraneous Bovine Viral Diarrhea Virus in

Modified-Live Vaccines

October 16,

2014

109 (295.5

KB)

Titration of Bovine Rhinotracheitis Neutralizing Antibody

(Constant Virus - Varying Serum Method)

October 16,

2014

110

(257.13

KB)

Titration of Eastern, Western, and Venezuelan Equine

Encephalomyelitis Virus Neutralizing Antibodies

April 05,

2016
ID Title Date

111

Titration of Infectious Bovine Rhinotracheitis Virus

Neutralizing Antibody (Constant Virus - Varying Serum

Method)

--

112

(255.58

KB)

Titration of Bovine Viral Diarrhea Neutralizing Antibody

(Constant Virus - Varying Serum Method)

October 16,

2014

113

(278.25

KB)

Titration of Parainfluenza-3 Neutralizing Antibody

(Constant Virus - Varying Serum Method)

May 12,

2017

114

(191.01

KB)

Titration of Porcine Transmissible Gastroenteritis Virus October 16,

2014

115

(183.31

KB)

Titration of Bovine Viral Diarrhea Neutralizing Antibody

(Constant Serum - Varying Virus Method)

October 16,

2014

116

(158.05

KB)

Phenotypic Examination of Pseudorabies Virus for

Thymidine Kinase Activity by a Plaque Selection Method

October 16,

2014

117 (254.5

KB)

Titration of Pseudorabies Virus Neutralizing Antibody

(Constant Virus - Varying Serum Methods)

October 16,

2014

118 (130.5

KB) Titration of Pseudorabies Virus in Vaccines August 7,2023

119

(172.12

KB)

Titration of Pseudorabies Virus Neutralizing Antibody

(Constant Virus - Varying Serum Method)

August 7,

2023

120

(170.47

KB)

In Vitro Potency Assay of Bovine Respiratory Viruses in

Killed Vaccines

October 16,

2014

121

(148.42

KB)

Titration of Porcine Rotavirus in Modified-live Vaccines October 21,

2014

122

(216.34

KB)

Titration of Porcine Rotavirus Antibody (Constant VirusVarying Serum Method)October 21,

2014
ID Title Date

123

(171.34

KB)

Pseudorabies Virus Challenge Test in Swine October 21,

2014

124

(226.66

KB)

Conducting the Hemagglutination Inhibition Assay for

Equine Influenza Antibody

October 23,

2014

126

(185.52

KB)

Titration of Bovine Rotavirus in Vaccines (Constant Virus -

Varying Serum Method)

October 23,

2014

200 Series: Anaerobic Bacteria Techniques

ID Title Date

200 (156.86

KB)

Potency Testing Products Containing Clostridium

chauvoei Antigen

December 13,

2017

201 (163.69

KB)

Potency Testing Clostridium perfringens Type C Beta

Antigen August 24, 2023

202 (158.49

KB)

Potency Testing Clostridium perfringens Type C Beta

Antitoxins

December 12,

2017

203 (166.81

KB)

Potency Testing Clostridium perfringens Type D

Epsilon Antigen August 24, 2023

204 (257.36

KB)

Potency Testing Clostridium perfringens Type D

Epsilon Antitoxins May 19, 2023

206 (203.06

KB) Potency Testing Tetanus Antitoxins December 18,2017

207 (275.62

KB)

Potency Testing Clostridium novyi Type B Alpha

Antigen August 10, 2023

209 (157.03

KB)

Potency Testing Products Containing Clostridium

haemolyticum Antigen

December 13,

2017

212 (166.54

KB) Potency Testing Clostridium sordellii Antigen August 10, 2023

213 (158.45

KB)

Potency Testing of Clostridium botulinum Type C

Bacterin-Toxoids June 17, 2022

214 (155.75

KB)

Immunogenicity and Spore Count Testing of Anthrax

Spore Vaccine-Nonencapsulated June 12, 2022
ID Title Date

217 (228.44

KB) Potency Testing of Tetanus Toxoid by ELISA June 17, 2022

300 Series: Small Animal Virus Techniques

ID Title Date

300

(185.81

KB)

Titration of Neutralizing Antibody - Canine Hepatitis August 22,

2023

301

(211.26

KB)

Titration of Neutralizing Antibody - Canine Distemper August 22,

2023

302

(212.99

KB)

Titration of Canine Distemper Virus in Primary Cell

Culture

October 29,

2014

303

(206.91

KB)

Titration of Distemper Virus in Embryonated Chicken

Eggs

October 30,

2014

304

(205.85

KB)

Titration of Infectious Canine Hepatitis Virus in Primary

Canine Kidney Cell Culture

October 30,

2014

305

(208.69

KB)

Titration of Feline Panleukopenia Virus in Cell Culture October  30,

2014

306

(179.35

KB)

Titration of Feline Calicivirus in Cell Culture October  30,

2014

307

(155.38

KB)

Titration of Feline Rhinotracheitis Virus in Cell Culture October 30,

2014

308

(176.85

KB)

Potency Testing of Inactivated Rabies Vaccine in Mice

Using the National Institutes of Health Test

March 31,

2015

309

(215.01

KB)

Titration of Canine Parainfluenza Virus in Vero Cell

Culture

October  30,

2014
ID Title Date

310 Scoring Feline Calicivirus in Cats Following Challenge --

311

Scoring Feline Rhinotracheitis Virus in Cats Following

Challenge --

312

(124.13

KB)

Detection of Extraneous Cytopathogenic Agents in

Master Seed Virus

October  30,

2014

313

(272.38

KB)

Detection of Extraneous Hemadsorbing Agents in Master

Seed Virus

August 23,

2023

314 (150.1

KB)

Detection of Extraneous Viruses by the Fluorescent

Antibody Test

November 13,

2014

315 (181

KB)

Detection of Serum Neutralizing Antibodies to Rabies

Virus using the Rapid Fluorescent Focus Inhibition Test

(RFFIT)

November 13,

2014

316

(205.34

KB)

Titration of Canine Parvovirus in Cell Culture November 14,

2014

317

(200.82

KB)

Titration of Canine Adenovirus in Canine Kidney Cell

Culture

November 13,

2014

318

Estimating Relative Potency by the Automated Selection

of Line Segments in RelPot 4.0 Software --

319 (307

KB)

Titration of Chlamydophila felis (formerly

Feline Chlamydia psittaci) in Cell Culture

November 13,

2014

320

(144.95

KB)

Titration of Feline Chlamydia psittaci in Embryonated

Chicken Eggs

November 13,

2020

321

(164.72

KB)

Quantitating the GP70 Antigen of Feline Leukemia Virus

in Veterinary Vaccines

January 9,

2015

322

(213.13

KB)

Determination of the Specific Viral Antigen Content in

Inactivated Canine Coronavirus Vaccines

January 9,

2015
ID Title Date

323

(205.35

KB)

Titration of Canine Distemper Virus in Vero Cell Culture November 20,

2020

400 Series: Poultry Virus Techniques

ID Title Date

405

(412.95

KB)

Detecting Lymphoid Leukosis Biocontamination by the

COFAL Test

November

28, 2014

406 (234

KB)

Titration of Monovalent, Cell Associated Marek′s Disease

Vaccines of Serotypes 1, 2 or 3

November

20, 2014

407

(218.48

KB)

Titration of Marek's Serotype 3 (Herpesvirus of Turkeys

Strain FC-126), Lyophilized

November

20, 2014

408

(158.28

KB)

Titrating Tissue Culture Adapted Vaccine Strain of Infectious

Bursal Disease Virus

November

28, 2014

409

(122.5

KB)

Titrating the Fractions of Combination Avian

Encephalomyelitis/Pox Vaccine

November

28, 2014

411

(157.13

KB)

Titration of Newcastle Disease Vaccine, Infectious Bronchitis

Vaccine, and Combination Newcastle Disease-Infectious

Bronchitis Vaccine in Chicken Embryos

January 10,

2018

500 Series: Chemical Techniques

ID Title Date

500 (1.01

MB)

Determination of Phenol in Veterinary Biologics (Pullorum

antigen, Mycoplasma synoviae antigen, and Mycoplasma

gallisepticum antigen)

June

16,

2022

502 (1.69

MB)

Determination of Residual Moisture in Veterinary Biologics

Products

June

17,

2022

504

(882.54

KB)

Manual Determination of Protein Content of Veterinary Biologics

(Biuret)

June

17,

2022
ID Title Date

509

Mikrozone Electrophoretic Separation of Blood Fractions Used in

Veterinary Biologics --

512

(162.32

KB)

Determination of Formaldehyde in Veterinary Biologics (Ferric

Chloride Test)

April 5,

2022

513 (3.51

MB)

Determination of Protein and Phenol in PPD Bovis Tuberculin

(Purified Protein Derivative Produced from Cultures

of Mycobacterium bovis (Strain AN-5))

June

17,

2022

514

(354.45

KB)

Determination of Hydrogen Ion Concentration, Total Nitrogen,

Phenol and Clarity in Intradermic Tuberculin (Filtrate Produced

From Cultures of Pn, C, and Dt Strains of Mycobacterium

tuberculosis)

June

17,

2022

600 Series: Aerobic Bacteria Techniques

ID Title Date

600

(224.18 KB)

Purity, Potency, and Dissociation of Brucella abortus

Vaccine, Strain 19 January 3, 2022

601 Potency Testing of Erysipelas Antiserum in Mice --

602 (46.36

KB) Evaluation of Salmonella Pullorum Antigens January 28,2010

603

(925.05 KB)

Potency Testing of Fowl Cholera (Pasteurella

multocida) Bacterins, Type 3 June 17, 2022

604 (1.09

MB) Evaluation of Koch's Old Tuberculin June 17, 2022

605 Potency Testing of Erysipelas Bacterins in Swine --

606 Potency Testing of Erysipelas Vaccines in Swine --

607

(929.34 KB)

Potency Testing of Fowl Cholera (Pasteurella

multocida) Bacterins, Type 1 June 17, 2022

608

(257.19 KB)

Potency Assay of Leptospira interrogans serogroup

pomona Bacterins

December 23,

2022

609

(254.96 KB)

Potency Assay of Leptospira interrogans serogroup

canicola Bacterins April 19, 2022

610

(242.52 KB)

Potency Assay of Leptospira interrogans serogroup

icterohaemorrhagiae Bacterins

December 23,

2022
ID Title Date

611

(221.17 KB) Potency Testing of Erysipelas Bacterins in Mice December 15,2017

612 (126.2

KB)

Bacterial Plate Count of Erysipelothrix rhusiopathiae

Vaccines

February 05,

2015

613

(906.22 KB)

In vitro Potency Testing of Erysipelothrix rhusiopathiae

Bacterins June 17, 2022

617

(225.91 KB)

Potency Assay Leptospira kirschneri serogroup

grippotyphosa Bacterins April 26, 2022

620

(256.57 KB)

Potency Testing of Enterotoxigenic (K99 Pilus)

Escherichia coli Bacterins May 26, 2023

621

(245.17 KB)

Potency Testing of Enterotoxigenic (K88 Pilus)

Escherichia coli Bacterins May 26, 2023

622

(246.24 KB)

Potency Testing of Enterotoxigenic (987P Pilus)

Escherichia coli Bacterins May 26, 2023

623

(248.28 KB)

Potency Testing Enterotoxigenic (F41 Pilus)

Escherichia coli Bacterins May 26, 2023

624

(215.31 KB)

In vitro Potency Testing of Leptospira interrogans

serogroup pomona Bacterins May 28, 2023

625

(229.39 KB)

In vitro Potency Testing of Leptospira interrogans

serogroup canicola Bacterins May 28, 2023

626

(216.91 KB)

In vitro Potency Testing of Leptospira kirschneri

serogroup grippotyphosa Bacterins May 28, 2023

627

(233.25 KB)

In vitro Potency Testing of Leptospira interrogans

serogroup icterohaemorrhagiae Bacterins May 28, 2023

630

(924.18 KB)

Potency Testing of Fowl Cholera (Pasteurella

multocida) Bacterins, Type 4 June 17, 2022

631

(933.65 KB) Potency Testing of Salmonella typhimurium Bacterins June 17, 2022

632

(936.52 KB) Potency Testing of Salmonella choler aesuis Bacterins June 17, 2022

633

(968.02 KB) Potency Testing of Salmonella dublin Bacterins June 17, 2022

634 (172.1

KB)

Potency Testing of Pasteurella multocida Bacterins of

Bovine Origin

December 18,

2017
ID Title Date

635

(217.46 KB)

Potency Testing of Pasteurella multocida Bacterins of

Porcine Origin

December 18,

2017

636 (1.01

MB)

Evaluation of Tuberculin Purified Protein Derivative

(PPD) June 17, 2022

800 Series: Cytology Techniques

ID Title Date

801 (160.39

KB)

Examination of Porcine Cells for Porcine Parvovirus

Contamination

November 28,

2014

803 (159.05

KB)

Examination of Trypsin Solutions for Porcine

Parvovirus Contamination

November 28,

2014

900 Series: Sterility Techniques

ID Title Date

900

(249.27

KB)

Testing Growth Promoting Qualities of Fluid Thioglycollate

Medium and Soybean-Casein Digest Medium using Bacillus

subtilis Spores and Issatchenkia orientalis as the Indicator

Organisms

March 19,

2015

901

(177.86

KB)

Testing Growth-Promoting Qualities of Fluid Thioglycollate

Medium with Beef Extract Using Clostridium chauvoei Spores

as the Indicator Organism

March 19,

2015

902

(158.6

KB)

Testing Growth-Promoting Qualities of Brain Heart Infusion

Agar using Bacillus subtilis Spores and Issatchenkia orientalis

 as Indicator Organisms

February

25, 2015

903

(210.16

KB)

Testing for Preservative Interference with Sterility Tests January 3,

2022

905

(227.51

KB)

Potency of Live Avirulent Mannheimia haemolytica Vaccine January 3,

2022

906

(256.34

KB)

Sterility Testing of Preparations Other Than Live Vaccines May 12,

2023

908

(252.27

KB)

Sterility Testing of Live Viral Vaccines and Master Seed Virus

Samples

May 12,

2023
ID Title Date

909

(230.64

KB)

Sterility Testing of Live Viral Vaccines of Chicken Embryo

Origin Recommended for Nonparenteral Injection

May 12,

2023

910

(248.48

KB)

Detection of Mycoplasma Contamination February

21, 2023

911

(250.03

KB)

Detection of Salmonella in Antibody Products April 27,

2023

912

(203.68

KB)

Determination of Total Bacterial Count in Antibody Products February

21, 2023

913

(230.76

KB)

Detection of Coliforms in Antibody Products April 27,

2023

914

(193.99

KB)

Detection of Fungi in Antibody Products May 15,

2023

918

(212.26

KB)

Bacterial Count of Pasteurella multocida, Avian Isolates,

Vaccines

February

21, 2023

928

(227.99

KB)

Detection of Extraneous Bacteria and Fungi in Live Bacterial

Vaccines and Master Seed Bacteria Samples

July 26,

2018