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ASF NVSL-FM-1083.04 FM-FADDL0041.03

Extraction of Total DNA from 

Tissues, Tissue Culture Supernatant, 

and Whole Blood Using a Qiagen 

DNeasy® Blood and Tissue Mini Kit 

Worksheet 

ASF NVSL-FM-1094.05 FM-FADDL0052.03

ASF PCR Control QC Summary 

Sheet 

ASF NVSL-FM-1129.06 FM-FADDL0087.04

African Swine Fever rPCR Assay on 

the Applied Biosystems 7500 Realtime PCR System Worksheet

ASF NVSL-FM-1134.03 FM-FADDL0092.01

African Swine Fever rPCR Assay on 

the Applied BiosystemsTM 

QuantStudioTM 5 Real-Time PCR 

System Worksheet

ASF NVSL-SOP-0591.04 SOP-FADDL0016.03

Extraction of Total DNA from 

Tissues, Tissue Culture Supernatant, 

and Whole Blood Using a Qiagen 

DNeasy® Blood and Tissue Mini Kit 

ASF NVSL-SOP-0646.05 SOP-FADDL0071.03

Preparation, Performance and 

Interpretation of the African Swine 

Fever rPCR Assay on the Applied 

Biosystems 7500 Real-time PCR 

System

ASF NVSL-SOP-0650.04 SOP-FADDL0075.02

Preparation, Performance, and 

Interpretation of the African Swine 

Fever rPCR Assay on the Applied 

Biosystems® QuantStudio 5 Realtime PCR System

ASF, CSF NVSL-MAN-0100.01 MAN-NAHLN0006.01

African Swine Fever (ASF)/Classical 

Swine Fever (CSF) Surveillance - 

NAHLN Laboratory Manual

ASF, CSF NVSL-FM-2124.01 NA

African Swine Fever and Classical 

Swine Fever rtPCR Assay on the 

Applied Biosystems TM QuantStudio 5 

TM and the Applied Biosystems TM 

7500 FAST Real-Time PCR Systems 

Worksheet

ASF, CSF NVSL-SOP-1201.02 NA

Preparation, Performance, and 

Interpretation of the African and 

Classical Swine Fever Real-Time 

Reverse-Transcription Multiplex 

Polymerase Chain Reaction (rRTPCR) on the Applied BiosystemsTM 

QuantStudioTM 5 Real-Time System 

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ASF, CSF NVSL-SOP-1203.02 NA

Preparation, Performance, and 

Interpretation of the African and 

Classical Swine Fever Real-Time 

Reverse-Transcription Multiplex 

Polymerase Chain Reaction (rRTPCR) on the Applied BiosystemsTM 

7500 Real-Time System

ASF, CSF, 

FMD NVSL-FM-1080.05 FM-FADDL0038.03

Recovery of Sample from a Swab 

Worksheet

ASF, CSF, 

FMD NVSL-FM-1081.05 FM-FADDL0039.03

Recovery of Sample from Tissue 

Worksheet 

ASF, CSF, 

FMD NVSL-FM-1127.04 FM-FADDL0085.02

Nucleic Acid Extraction Using the 

MagMAXTM CORE Nucleic Acid 

Purification Kit on a Magnetic Particle 

Processor Worksheet

ASF, CSF, 

FMD NVSL-FM-1128.06 FM-FADDL0086.04

Nucleic Acid Extraction Using the 

MagMAXTM Pathogen RNA/ DNA Kit 

on a Magnetic Particle Processor 

Worksheet

ASF, CSF, 

FMD NVSL-SOP-0589.03 SOP-FADDL0014.03

Preparation of Tissues, Tissue 

Homogenates, and Swabs for 

Nucleic Acid Extraction 

ASF, CSF, 

FMD NVSL-SOP-0643.04 SOP-FADDL0068.03

Nucleic Acid Extraction Using the 

MagMAXTM CORE Nucleic Acid 

Purification Kit on a Magnetic Particle 

Processor

ASF, CSF, 

FMD NVSL-SOP-0644.04 SOP-FADDL0069.03

Nucleic Acid Extraction using the 

MagMAX Pathogen RNA/DNA kit on 

a Magnetic Particle Processor

ASF, CSF, 

FMD NVSL-SOP-0645.02 SOP-FADDL0070.01

Nucleic Acid Extraction Using the 

INDICAL BIOSCIENCE IndiMag 

Pathogen Kit on a Magnetic Particle 

Processor

ASF, CSF, 

FMD NVSL-SOP-1059.05 SOP-RVS0028.03

Production and Use of a Reference 

Panel for Quality Control of Primers 

and Probes for PCR 

ASF, CSF, 

FMD NVSL-WI-1174.03 WI-RVS0003.02

Work Instructions for Use of a 

Reference Panel for QA/QC Testing 

of Primers and Probes for Real-time 

PCR Applications

BSE NVSL-SOP-0886.02 SOP-PS0010.02

Redirection of Bovine Spongiform 

Encephalopathy Samples 

BSE NVSL-SOP-0924.02 SOP-PS0048.02

Quality Assurance: Proficiency 

Testing and Quality Monitoring of 

BSE ELISA Contract Laboratories by 

NVSL 

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BSE NVSL-SOP-0927.02 SOP-PS0051.02

Protocol for Bovine Spongiform 

Encephalopathy (BSE) Approved 

National Animal Health Laboratory 

Network (NAHLN) Laboratories to 

Receive and Test Bovine Brain 

Samples and Report Results for BSE 

Surveillance 

CSF NVSL-FM-1093.05 FM-FADDL0051.03

CSF PCR Control QC Summary 

Sheet 

CSF, FMD NVSL-FM-1126.03 FM-FADDL0084.01

Classical Swine Fever and Foot and 

Mouth Disease Real-Time RT-PCR 

Assay Using the TaqMan® Fast 

Virus 1-Step Master Mix on the 

Applied Biosystems Quant Studio 5 

Real-time PCR Platform Worksheet

CSF, FMD NVSL-FM-1130.07 FM-FADDL0088.05

Classical Swine Fever and Foot and 

Mouth Disease rRT-PCR Using the 

TaqMan® Fast Virus 1-Step Master 

Mix on the Applied Biosystems 7500 

Real-time PCR Platform Worksheet

CSF, FMD NVSL-FM-1131.03 FM-FADDL0089.01

Nucleic Acid Extraction Using the 

INDICAL BIOSCIENCE IndiMag 

Pathogen Kit on a Magnetic Particle 

Processor Worksheet

CSF, FMD NVSL-SOP-0642.07 SOP-FADDL0067.06

Classical Swine Fever and Foot and 

Mouth Disease rRT-PCR Using the 

TaqMan® Fast Virus 1-Step Master 

Mix on the Applied Biosystems 7500 

Real-time PCR Platform

CSF, FMD NVSL-SOP-0647.02 SOP-FADDL0072.01

Classical Swine Fever and Foot and 

Mouth Disease rRT-PCR Using the 

TaqMan® Fast Virus 1-Step Master 

Mix on the Applied Biosystems 

QuantStudio 5 Real-time PCR 

Platform

CWD NVSL-SOP-0883.02 SOP-PS0007.02

Chronic Wasting Disease (CWD) 

Enzyme-Linked Immunosorbent 

Assay (ELISA) 

CWD 

ELISA, BSE 

ELISA

NVSL-FM-1583.04 FM-PS-0002.04 Information on "Initial Reactives” for 

TSE ELISA 

CWD IHC / 

Scrapie IHC NVSL-SOP-0878.03 SOP-PS0002.03

Detection of Scrapie and Chronic 

Wasting Disease by 

Immunohistochemistry (IHC)

Fin Fish – VI 

(VHSV & 

SVCV)

NVSL-FM-0393.03 FM-BPA0005.03

24 Well Plate Virus Isolation 

Worksheet 

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Fin Fish – VI 

(VHSV & 

SVCV)

NVSL-FM-0408.04 FM-BPA0020.02 Virus Isolation Worksheet 

Fin Fish – VI 

(VHSV & 

SVCV), 

VHS-PCR, 

ISA-PCR

NVSL-SOP-0285.05 SOP-BPA0014.05

Isolation of Viral Agents from Finfish 

in Tissue Cell Culture 

Fin Fish – VI 

(VHSV & 

SVCV), 

VHS-PCR, 

ISA-PCR

NVSL-SOP-0324.03 SOP-BPA0053.03

Disruption of Fish Tissue by MicroBead Beating 

FMD NVSL-FM-1083.04 FM-FADDL0041.03 

Extraction of Total DNA from 

Tissues, Tissue Culture Supernatant, 

and Whole Blood using a Qiagen 

DNeasy® Blood and Tissue Mini Kit 

Worksheet

FMD NVSL-FM-1092.04 FM-FADDL0050.03

FMD PCR Control QC Summary 

Sheet 

FMD/SVA 

Multiplex NVSL-FM-1132.03 FM-FADDL0090.01

Foot and Mouth Disease Virus and 

Senecavirus A Multiplex rRT-PCR 

Using the TaqMan® Fast Virus 1-

Step Master Mix on the Applied 

Biosystems 7500 Real-time PCR 

Platform Worksheet

FMD/SVA 

Multiplex NVSL-SOP-0648.02 SOP-FADDL0073.01

Preparation, Performance and 

Interpretation of the Foot and Mouth 

Disease Virus and Senecavirus A 

Multiplex rRT-PCR Assay on the 

Applied Biosystems 7500 FAST Realtime PCR System

IAV-A NVSL-SOP-0018.05 SOP-AV0018.04

Isolation of Avian Influenza and Avian 

Paramyxoviruses Viruses in Chicken 

Embryos from Avian Species

IAV-A NVSL-WI-0042.05 WI-AV-0039.04

Quick Reference for Real-Time RTPCR Influenza A Detection using the 

IAV M 2009 Assay (for AB7500 Fast 

and Cepheid SmartCycler II) 

IAV-A NVSL-WI-0048.06 WI-AV-0045.05 NVSL Testing Guidance for Post 

C&D Environmental Samples 

IAV-A NVSL-WI-0053.02 WI-AV-0050.01

Quick Reference for Real-Time RTPCR Influenza A Assays for Subtype 

H5 or H7 Detection (for AB7500 Fast 

and Cepheid Smart Cycler) 

IAV-A, IAVSNVSL-Process0002.02Process-DVL0001Influenza A Test Comparison for 

Avian and Swine

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IAV-A, IAVS, ND NVSL-WI-0054.04 WI-AV-0051.03

Evaluation and Quality Control 

Testing of Real Time PCR Primers 

and Probes

IAV-A, IAVS, ND NVSL-WI-0055.02 WI-AV-0052.01 RNA Extraction Using a Magnetic 

Particle Processor 

IAV-A, ND NVSL-FM-0039.04 FM-AV-0038.03 RRT-PCR Primer and Probe Quality 

Control Worksheet 

IAV-A, ND NVSL-Process0001.02

Process-AV0001.01Testing and Confirmation for Avian 

influenza and Newcastle Disease

IAV-A, ND NVSL-SOP-0068.04 SOP-AV0068.04

Real-time RT-PCR Detection of 

Influenza A and Avian Paramyxovirus 

Type-1

IAV-A, ND NVSL-WI-0023.11 WI-AV-0020.10 Avian Sample Collection for Influenza 

A and Newcastle Disease 

IAV-A, ND NVSL-WI-0038.06 WI-AV-0035.05

Programming and Analysis for the 

Cepheid SmartCycler Real-Time 

Instrument 

IAV-A, ND NVSL-WI-0039.05 WI-AV-0036.04

Programming and Analysis for the 

Roche LightCycler® 480 Real-Time 

Instrument 

IAV-A, ND NVSL-WI-0040.04 WI-AV-0037.03

Programming and Analysis for the 

Applied Biosystems 7900 Real-Time 

Instrument 

IAV-S NVSL-SOP-0294.02 SOP-BPA0023.02 Swine Influenza Virus Isolation 

IAV-S NVSL-SOP-0315.04 SOP-BPA0044.03

Real-Time RT-PCR for Subtyping 

Type A Swine Influenza Virus in 

Clinical Samples 

IAV-S NVSL-WI-0209.03 WI-BPA0032.02

Minimum Requirements for 

Submitting NAHLN Swine Influenza 

Virus Sequencing to GenBank 

IAV-S NVSL-SOP-0289.03 SOP-BPA0018.02

Real-Time RT-PCR for the Detection 

of Influenza A Virus in Swine (IAV-S) 

in Clinical Samples 

IAV-S NVSL-SOP-0290.03 SOP-BPA0019.03

Amplification and Purification of PCR 

Products for Direct Sequencing of 

Hemagglutinin, Neuraminidase, and 

Matrix Genes of Swine Influenza 

Virus 

ISA-PCR NVSL-FM-0479.05 FM-BPA0091.03 ISAV RT-PCR 

ISA-PCR NVSL-FM-0480.03 FM-BPA0092.02

KingFisher AMB1836 Extraction 

Worksheet for Aquaculture 

ISA-PCR NVSL-FM-0481.03 FM-BPA0093.02

ISAV VetMAX plus - Multiplex One 

Step RT-PCR 

ISA-PCR NVSL-SOP-0323.02 SOP-BPA0052.02

Dissection of Fish for Detection of 

Viruses During Large-Scale, 

Targeted Surveillance for ISAV 

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ISA-PCR NVSL-SOP-0326.03 SOP-BPA0055.03

Infectious Salmon Anemia Real-time 

RT-PCR - segment 8 Snow primers 

ISA-PCR NVSL-SOP-0327.03 SOP-BPA0056.03

Infectious Salmon Anemia RT-PCR - 

Segment 6 and 8 

N/A NVSL-FM-1347.04 FM-NAHLN0051.03

NAHLN Quality Standard 

Harmonization Checklist

N/A NVSL-MAN-0028.04 MAN-NAHLN0002.03

NAHLN Laboratory Guide for Foreign 

Animal Disease (FAD) Investigations

N/A NVSL-Policy-0036.02 Policy-NAHLN0006.01 NAHLN Laboratory Performance 

N/A NVSL-Policy-0037.03 Policy-NAHLN0007.02

NAHLN Quality Standard - 

Harmonization of AAVLD and 

ISO/IEC 17025:2017

N/A NVSL-WI-0702.06 WI-NAHLN0011.04

NAHLN Laboratory Controlled Copy 

SOP Responsibilities 

N/A NVSL-WI-0721.05 WI-NAHLN0030.05

Registering for a Proficiency Test 

(PT) Event in the APHIS Laboratory 

Portal

N/A NVSL-WI-0722.01 WI-NAHLN0031.01

Proficiency Test Management in 

NAHLN Portal

N/A NVSL-WI-0729.08 WI-NAHLN0038.07 NAHLN Test Reference Chart

ND NVSL-WI-0041.04 WI-AV-0038.03

Quick Reference for Real-Time RTPCR Avian Paramyxovirus Type-1 

Detection (for AB7500 Fast and 

Cepheid SmartCycler) 

VHS-PCR NVSL-FM-0480.03 FM-BPA0092.03

KingFisher AMB1836 Extraction 

Worksheet for Aquaculture

VHS-PCR NVSL-FM-0482.04 FM-BPA0094.03 VHSV rRT-PCR Worksheet 

VHS-PCR NVSL-SOP-0331.02 SOP-BPA0060.02

Viral Hemorrhagic Septicemia Virus 

Real-time RT-PCR 

VHS-PCR, 

ISA-PCR NVSL-FM-0458.04 FM-BPA0070.03

MagMAX AM1836 Extraction 

Worksheet for Aquaculture 

VHS-PCR, 

ISA-PCR NVSL-SOP-0325.04 SOP-BPA0054.04

Extraction of Nucleic Acid (DNA and 

RNA) from Fish Tissues - AM1836 

VSV NVSL-FM-0405.04 FM-BPA0017.03

Vesicular Stomatitis Complement 

Fixation Bench Record 

VSV NVSL-FM-0406.04 FM-BPA0018.02

Complement Fixation Assay for 

Vesicular Stomatitis Complement 

Titration Worksheet 

VSV NVSL-FM-0407.04 FM-BPA0019.03

Complement Fixation Assay for 

Vesicular Stomatitis

Hemolysin Titration Worksheet 

VSV NVSL-FM-0420.04 FM-BPA0032.02

Washing & Standardization of Sheep 

Red Blood Cells 

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VSV NVSL-FM-0483.04 FM-BPA0095.02

Vesicular Stomatitis PCR Worksheet 

Using Fast Virus 1-Step Kit 

VSV NVSL-FM-0485.04 FM-BPA0097.03

MagMAX Express 96 Extraction 

Worksheet (200μl sample) 

VSV NVSL-SOP-0281.03 SOP-BPA0010.02

Complement Fixation Test for 

Detection of Antibodies to Vesicular 

Stomatitis Virus 

VSV NVSL-SOP-0282.03 SOP-BPA0011.02

Reagent Preparation and Evaluation 

for the Vesicular Stomatitis 

Complement Fixation Assay 

VSV NVSL-SOP-0334.04 SOP-BPA0063.03

Real-time Reverse-Transcriptase 

Polymerase Chain Reaction for the 

Detection of Vesiculovirus Nucleic 

Acids Using the ABI 7500 Detection 

System

VSV NVSL-WI-0225.02 WI-BPA0048.02

RNA Extraction of Vesicular 

Stomatitis Using MagMAX Bead Kits 

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Revision Document Title

NVSL-SOP-0014 07 Intravenous Pathogenicity Assessments for Avian Influenza 

Virus in Chickens

NVSL-SOP-0018 05 Isolation of Avian Influenza and Avian Paramyxoviruses 

Viruses in Chicken Empryos from Avian Species

NVSL-SOP-0066 01 Agar Gel Immunodiffusion Test to Detect Antibodies to Avian 

Encephalomyelitis Virus

NVSL-SOP-0068 04 Real-time RT-PCR Detection of Influenza A and Avian 

Paramyxovirus Type-1 

NVSL-SOP-0074 02 Avian Influenza Real-Time RT-PCR Testing for Qualified NPIP 

Primary Breeder Authorized Laboratories

NVSL-SOP-0076 05 Quality Control Testing of Taylorella spp. Media

NVSL-SOP-0082 05 Pulsed Field Gel Electrophoresis (PFGE) on Taylorella isolates

NVSL-SOP-0089 04 Salmonella pullorum Microagglutination Test

NVSL-SOP-0091 04 Salmonella pullorum Tube Agglutination Test

NVSL-SOP-0180 07 Taylorella spp. Isolation and Identification

NVSL-SOP-0181 04 Taylorella spp. Direct Fluorescent Antibody Test

NVSL-SOP-0183 05 Real-time Polymerase Chain Reaction Test for Rapid 

Identification and Speciation of Taylorella spp.

NVSL-SOP-0292 01 Virus Isolation Procedure for Pseudorabies Virus (PRV)

NVSL-SOP-0306 06 Virus Neutralization Test for Detection of Antibodies Against 

Pseudorabies Virus

NVSL-SOP-0310 05 Serology Testing for Pseudorabies in the Diagnostic Virology 

Laboratory

NVSL-SOP-0323 02 Dissection of Fish for Detection of Viruses During Large-Scale, 

Targeted Surveillance for ISAV

NVSL-SOP-0324 03 Disruption of Fish Tissue by Micro-Bead Beating

NVSL-SOP-0325 04 Extraction of Nucleic Acid (DNA and RNA) from Fish Tissues - 

AM1836

NVSL-SOP-0326 03 Infectious Salmon Anemia Real-time RT-PCR - segment 8 

Snow primers

NVSL-SOP-0327 03 Infectious Salmon Anemia RT-PCR - Segment 6 and 8

NVSL-SOP-0331 02 Viral Hemorrhagic Septicemia Virus Real-time RT-PCR

NVSL-SOP-0532 03 Polymerase Chain Reaction Test for Bluetongue Virus and 

Epizootic Hemorrhagic Disease Virus

NVSL-SOP-0533 03 Serotype Identification of Orbiviruses (BTV, EHDV, and AHSV) 

by RT-PCR

NVSL-SOP-0536 03 Isolation of Bluetongue Virus

NVSL-SOP-0538 02 Equine Arteritis Virus Isolation

NVSL-SOP-0541 02 General Protocol for Isolation of Equine Viruses

NVSL-SOP-0558 02 Microtiter Virus Neutralization Test for Equine Viral Arteritis

NVSL-SOP-0560 03 Western blot Test for Equine Infectious Anemia

NVSL-SOP-0562 02

Requirements for Equine Infectious Anemia Agar Gel 

Immunodiffusion (Coggins) and Enzyme-Linked 

Immunosorbent Assay Testing at Veterinary Services Approved 

Laboratories

NVSL-SOP-0564 02 Virus Neutralization Test for Bluetongue and Epizootic 

Hemorrhagic Disease Viruses

NVSL-SOP-0569 06

Real-Time RT-PCR Assays for Detection of Bluetongue, 

Epizootic Hemorrhagic Disease and African Horse Sickness 

Viruses

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NVSL-SOP-0571 03 Procedure for Performing Miscellaneous Gel-based PCR 

Assays: EAV; Bunyavirus; and EHV-2, -3, & -5

NVSL-SOP-0591 04

Extraction of Total DNA from Tissues, Tissue Culture 

Supernatant, and Whole Blood Using a Qiagen DNeasy® 

Blood and Tissue Mini Kit

NVSL-SOP-0616 01 Nucleic Acid Sequencing of the Foot and Mouth Disease S 

Fragment

NVSL-SOP-0617 02 Nucleic Acid Sequencing of the Foot and Mouth Disease L 

Fragment

NVSL-SOP-0618 01 Nucleic Acid Sequencing of the Foot and Mouth Disease P1 

Region

NVSL-SOP-0619 01 Nucleic Acid Sequencing of the Foot and Mouth Disease 3’ 

Region

NVSL-SOP-0642 07

Classical Swine Fever and Foot and Mouth Disease rRT-PCR 

Using the TaqMan® Fast Virus 1-Step Master Mix on the 

Applied Biosystems 7500 Real-time PCR Platform

NVSL-SOP-0644 04 Nucleic Acid Extraction using the MagMAX Pathogen 

RNA/DNA kit on a Magnetic Particle Processor

NVSL-SOP-0659 03 Testing Requirements for Culture Media; Brucella spp. Isolation

NVSL-SOP-0660 03 Isolation: Brucella spp.

NVSL-SOP-0662 03 Evaluation of Individual Lots of Brucella Ring Test Antigen

NVSL-SOP-0664 03 Procedure for the Propagation of Brucellaphages

NVSL-SOP-0668 03 AMOS PCR

NVSL-SOP-0670 04 Identification of Brucella species

NVSL-SOP-0681 02 Bead BeaterTM DNA Extraction Method for Mycobacterial DNA

NVSL-SOP-0682 03 16s rDNA, rpoB, hsp65 and ITS Diagnostic Sequencing for 

Mycobacterium species

NVSL-SOP-0690 06 VersaTrekTM Liquid Culture of Mycobacterium species 

NVSL-SOP-0692 06 HPC Decontamination of Tissues Submitted for Mycobacteria 

Culture

NVSL-SOP-0693 04 HPC Decontamination of Fecal Swabs and Feces Submitted for 

Mycobacteria Culture

NVSL-SOP-0695 02 Procedures for Brucella Milk Ring Tests

NVSL-SOP-0696 07 NaOH Decontamination Method for Mycobacterial Culture

NVSL-SOP-0697 01

Direct PCR Assay of Feces for Detection of Mycobacterium 

avium subsp. paratuberculosis Using Bead Based Sample 

Disruption MagMaxTM Total Nucleic Acid Isolation Method

NVSL-SOP-0701 06 Inactivation of Brucella

NVSL-SOP-0708 02 Isolation of Mycobacteria Using Solid Media

NVSL-SOP-0710 05 MGITTM 960 Liquid Culture of Mycobacteria species

NVSL-SOP-0878 03 Detection of Scrapie and Chronic Wasting Disease by 

Immunohistochemistry (IHC)

NVSL-SOP-0882 03 Scrapie Enzyme-Linked Immunosorbent Assay (ELISA)

NVSL-SOP-0927 02

Protocol for Bovine Spongiform Encephalopathy (BSE) 

Approved National Animal Health Laboratory Network (NAHLN) 

Laboratories to Receive and Test Bovine Brain Samples and 

Report Results for BSE Surveillance

NVSL-SOP-0930 03 Detection of Mycobacterial Nucleic Acid in Formalin-Fixed 

Paraffin-Embedded Tissues by Polymerase Chain Reaction 

NVSL-SOP-0963 01 Preparation of Antiserum for the Avian Influenza Agar Gel 

Immunodiffusion Test

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NVSL-SOP-1033 08 Standard Operating Procedure (SOP) for Cell Culture Media 

Preparation

NVSL-SOP-1035 07 Standard Operating Procedure (SOP) for Production of Virus 

Stocks Used in the Various Virus Neutralization (VN) Tests 

NVSL-SOP-1047 02

Standard Operating Procedure (SOP) for Quality Control 

Testing of Virus Stocks Prepared by the Reagents and Vaccine 

Services Section

NVSL-SOP-1050 05 Classical Swine Fever 96 Well Plate Preparation for the 

Immunoperoxidase Test

NVSL-SOP-1051 06

Enzyme Linked Immunosorbent Assay (ELISA) for Titrating 

Rabbit and Guinea Pig Antisera Against Vesicular Disease 

Agents

NVSL-SOP-1052 05 Enzyme Linked Immunosorbent Assay (ELISA) for Testing 

Specificity of Rabbit Antisera Against Vesicular Disease Agents

NVSL-SOP-1053 05

Enzyme Linked Immunosorbent Assay (ELISA) for Testing 

Specificity of Guinea Pig Antisera Against Vesicular Disease 

Agents

NVSL-SOP-1062 07 Standard operating Procedure for Foot-and Mouth Disease 

(FMD) RT-qPCR Proficiency Panel Assembly and Distribution 

NVSL-SOP-1069 06 Standard operating Procedure for Classical Swine Fever (CSF) 

rRT-PCR Proficiency Panel Assembly and Distribution

NVSL-SOP-1071 06

Standard Operating Procedure (SOP) of Demonstration of 

Contagious Bovine Pleuropneumonia in Foreign Animal 

Disease Diagnostician Course

NVSL-SOP-1077 03

Standard Operating Procedure for the Application and 

Authorization for the Use of Biological Materials Containing 

Rinderpest Virus (RPV)

NVSL-SOP-1078 02

Handling, Packaging, Transfer, and Shipping of Rinderpest 

Virus (RPV) Containing Materials from the Reagents and 

Vaccine Services Section (RVSS) RPV Holding Facility

NVSL-SOP-1080 02

General Procedures for Manipulating Rinderpest virus (RPV)-

Containing Materials in the Reagents and Vaccines Service 

Section (RVSS) of the Foreign Animal Disease Laboratory 

(FADDL)

NVSL-SOP-1084 03 Workflow for Receipt and Transfer of DNA Materials for NaOH 

Safety Treatment

NVSL-SOP-1085 06 Disinfectants and Cleaners Used in the Reagents and Vaccines 

Services Section (RVSS)

NVSL-SOP-1087 05 Standard Operating Procedure (SOP) for Preparation of 

Vesicular Exanthema of Swine Virus (VESV) Slides

NVSL-SOP-1092 05

Production of Antisera against Antigens of Foot and Mouth 

Disease Virus (FMDV), Swine Vesicular Disease Virus (SVDV), 

and Vesicular Stomatitis Virus (VSV)

NVSL-SOP-1094 05 Workflow for Receipt, Process and Transfer of DNA Materials 

for NaOH Safety Treatment using Safety Treatment Database 

NVSL-SOP-1095 03 Solid Phase Competition ELISA for Detecting Antibodies 

against Foot-and-Mouth Disease Virus

NVSL-SOP-1101 04

Production of Recombinant Foot-and-Mouth Disease Virus 

(FMDV) 3D RNA Dependent RNA Polymerase for Virus 

Infection Associated Antigen (VIAA) Test

NVSL-SOP-1102 03

Propagation of Foot and Mouth Disease Virus (FMDV), Swine 

Vesicular Disease Virus (SVDV) and Vesicular Stomatitis Virus 

(VSV) in Roller Bottles for Antigen Purification

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NVSL-SOP-1106 03 Standard Operating Procedure (SOP) for Preparation of African 

Swine Fever Virus (ASFV) Slides

NVSL-SOP-1107 03

Production of Binary Ethyleneimine (BEI) Inactivated Positive 

Amplification Controls (PAC) and Positive Extraction Controls 

(PEC)

NVSL-SOP-1108 03 Purification of Vesicular Stomatitis Virus (VSV) Antigen

NVSL-SOP-1109 05 Inactivation of African Swine Fever Virus (ASFV) in Tissue 

Culture Supernatant using 3mM Binary Ethylenimine (BEI)

NVSL-SOP-1111 03

Preparation, Performance, and Interpretation of a Real-Time 

PCR Assay for the Detection of Capripoxviruses on ABI 7500 

Fast Thermocycler Using TaqMan Fast Virus One-step Master 

Mix

NVSL-SOP-1116 03

Complement Fixation Test for Detection of Antibodies to 

Babesia caballi and Theileria (Babesia) equi—Microtitration 

Test

NVSL-SOP-1117 07 Indirect Fluorescent Antibody Test for Detection of Antibodies 

to Babesia caballi and Theileria (Babesia) equi

NVSL-SOP-1123 02 Complement Fixation Test for Detection of Antibodies to 

Anaplasma marginale—Microtitration Test

NVSL-SOP-1125 03 Johne's Complement Fixation Test

NVSL-SOP-1128 03 Complement Fixation Test for Detection of Antibodies to 

Brucella abortus and Brucella suis

NVSL-SOP-1129 07 Plate Agglutination Test for Detection of Antibodies to Brucella 

abortus/suis

NVSL-SOP-1130 05 Buffered Acidified Plate Antigen Test for Detection of 

Antibodies to Brucella 

NVSL-SOP-1132 04 Tube Agglutination Test for Detection of Antibodies to Brucella 

abortus/suis

NVSL-SOP-1136 05 Performance of ID Screen Brucellosis Serum Indirect Multispecies ELISA

NVSL-SOP-1140 05 Card Test for Detection of Antibodies to Brucella

NVSL-SOP-1142 05 Anaplasma Antibody Test Kit, cELISA v2

NVSL-SOP-1144 10 Brucella Fluorescence Polarization Assay (FPA) Using Tubes

NVSL-SOP-1145 03 VMRD cELISA Kits for Babesia caballi and Babesia equi

NVSL-SOP-1151 06 IDEXX Johne's Disease ELISA Kit

NVSL-SOP-1152 05 Contagious Equine Metritis Complement Fixation Test

NVSL-SOP-1154 04 IDEXX ELISA Antibody Test Kits for Mycobacterium bovis

NVSL-SOP-1158 04 Johne's Agar Gel Immunodiffusion Test

NVSL-SOP-1164 05 Mycobacterium bovis Gamma Interferon Test Kit for Cattle 

NVSL-SOP-1193 01 Preparation of African Swine Fever Virus (ASFV) Antigen 

Plates and Strips for Antibody Detection

NVSL-SOP-1201 02

Preparation, Performance, and Interpretation of the African and 

Classical Swine Fever Real-Time Reverse-Transcription 

Multiplex Polymerase Chain Reaction (rRT-PCR) on the 

Applied BiosystemsTM QuantStudioTM 5 Real-Time System 

NVSL-SOP-1203 02

Preparation, Performance, and Interpretation of the African and 

Classical Swine Fever Real-Time Reverse-Transcription 

Multiplex Polymerase Chain Reaction (rRT-PCR) on the 

Applied BiosystemsTM 7500 Real-Time System

12/1/2022

Contact nvsl.mastercontrol@usda.gov for inquiries on 

Program Disease and APHIS-Approved Laboratory Documents. 11 of 11