Does Not Contain Confidential Business Information (CBI)

2011_June_Progress Report 1 PROGRESS REPORT: JUNE 2011

BIODEGRADATION TESTING SUPPLEMEN TAL ENVIRONMENTAL PROJECT

Submitted to

E.I du Pont de Nemours and Company

Wilmington, Delaware 19898

U.S.A.

United States Environmental Protection Agency

Washington, DC

U.S.A.



# Wildlife International, Ltd.

8598 Commerce Drive

Easton, Maryland 21601 USA

1 (410) 822-8600

June 29, 2011

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2011_March _Progress Report 2 Table of Contents

Title Page ..................................................................................................................... ..............1

Table of Contents.............................................................................................................. .........2

Introduction................................................................................................................... .............3

Progress Summary ............................................................................................................... ......3

Appendix A. Invoice........................................................................................................... ......4

Appendix B. Task E: Modifications and Refinements to Test Methods .................................6Does Not Contain Confidential Business Information (CBI)

2011_March _Progress Report 3 Introduction

This document presents the pr ogress of the technical work for the Biodegradation Testing

Supplemental Environmental Project (“Biodegr adation SEP”) performed from 28 May 2011

up to 24 June 2011. Studies conducted under protocol will be examined by the Wildlife

International Quality Assurance Unit for compliance with Good Laboratory Practices,

Standard Operating Procedures and the specifie d protocol. Raw data that has been audited

by the Wildlife International Quality Assurance will be included in the progress report, as

appropriate.

Progress Summary

The total costs associated with the project for the period covered in this Progress Report are

approximately $3,040 for labor and $18,900 for in strumentation. The invoice and an

allocation of costs with associated tasks are presented in Appendix A.

Work conducted over the period covered by this progress report consisted of the review and

discussion of data from test substance characterization and di scussion of Task E items with

stakeholders. Work also included on-going revi sions of draft method validation protocols,

and preparation of a Scope of Work to eval uate the analysis of 6-2 FTOH, 8-2 FTOH and

10-2 FTOH in MTBE extracts of an activated sludge matrix by liquid chromatography mass

spectrometry in the presence and absence of the Group A1 polymer (Appendix B).Does Not Contain Confidential Business Information (CBI)

2011_March _Progress Report 4 Appendix A

InvoiceDoes Not Contain Confidential Business Information (CBI)2011_June_Progress Report5Allocation of CostsMonth, Year Tasks TotalDesign andProcurement ofSCAS Units(Task A)Acquisitionof Standards(Task B)Adaptation ofAnalyticalMethod (Task C)Pilot StudiesTask (D)Refinementof MethodsTask (E)MethodValidations(Task F)StorageStability(Task G)BiodegradationStudies(Task H)InstrumentLeaseDec 2009 $1,803 $1,085 $42,422 $45,310Jan 2010 $29,227 $29,227Feb 2010 $1,300 $36,745 $38,045Mar 2010 $14,350 $14,350 $18,900Apr 2010 $570 $15,935 $16,505 $18,900May 2010 $760 $71,860 $72,620 $18,900June 2010 $57,870 $35,003 $92,873 $18,900July 2010 $42,430 $15,130 $57,560 $18,900August 2010 $32,530 $113,718 $146,248 $18,900September 2010 $1,350 $115,228 $116,578 $18,900October 2010 $88,823 $3,613 $92,478 $18,900November 2010 $42,690 $855 $570 $44,115 $18,900December 2010 $18,385 $3,530$3040 $24,955 $18,900January 2011 $15,570 $15,570 $18,900February 2011 $2,945 $2,945 $18,900March 2011 $8,075 $8,075 $18,900April 2011 $9,820 $9,820 $18,900May 2011 $5,770 $5,770 $18,900June 2011 $2,850 $190 $3,040 $18,900Labor Total $1,803 $3,715 $344,719 $428,988 $53,028 $3,800 $836,084Materials $6,800 $10,500 $20,644 $1,724 $39,669Total Cost $8,603 $14,215 $365,363 $430,712 $53,208 $3,800 $875,753 $302,400Does Not Contain Confidential Business Information (CBI)

2011_June_Progress Report 6 Appendix B. Task E: Modifications and Refinements to Test Methods