Title

Amended Application for an Experimental Use Permit for 1507x59122xMON810, 

1507xMON810 and 59122xMON810 

Data Requirement

40 CFR Part 172 

Authors

Rachel Binning, Keri Henderson, Dave Langer, Scott Mundell, Tracy Rood and Jamie Staley 

Submission Date

October 30, 2009 

Submitter

Jamie Staley 

Pioneer Hi-Bred International, Inc. 

2450 SE Oak Tree Court 

Ankeny, IA 50021 

Submitter Reference Number

PHI-2008-139 

Volume 1 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 1 of 500

 

Table of Contents 

Page

Statement of Data Confidentiality Claim.............................................................................................. 2 

Transmittal Document ........................................................................................................................ 5 

EPA Form 8570-17 (Application for Experimental Use Permit) ......................................................... 7 

EPA Form 8570-4 (Confidential Statements of Formula).................................................................... 8 

EPA Form 8570-34 (Certification with Respect to Citation of Data)................................................... 9 

EPA Form 8570-35 (Data Matrix for 1507x59122xMON810) ........................................................... 10 

EPA Form 8570-35 (Data Matrix for 1507xMON810) .......................................................................73 

EPA Form 8570-35 (Data Matrix for 59122xMON810) ...................................................................114 

Section A. Product Chemistry ........................................................................................................155 

Section B. Proposed Labels ...........................................................................................................156 

Section C. Reference to Available Environmental Toxicity Data....................................................212 

Section D. Residue Data ................................................................................................................213 

Section E. Effectiveness Data ........................................................................................................214 

Section F. Tolerance if Testing Involves Use in Food or Feed ......................................................218 

Section G. Proposed Experimental Program .................................................................................219 

G1. Participants in the Experimental Program.........................................................................219 

G2. Acreage and Seed Quantities by State or Territory for the EUP Program .......................219 

G3. Details of the Proposed EUP Program .............................................................................232 

G4. Definitions..........................................................................................................................232 

G5. Universal Protocol Procedures..........................................................................................233

Nursery / Breeding Protocol......................................................................................................236 

Yield / Agronomic Protocol........................................................................................................237 

Efficacy / IRM Protocol..............................................................................................................238 

Seed Production Protocol .........................................................................................................239 

Regulatory Protocol ..................................................................................................................240 

Confidential Attachment ..................................................................................................................241 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 3 of 500
 

List of Tables and Figures 

Tables 

Page

Table 1 Cross-reference Event Table ............................................................................................155 

Table 2. Lepidopteran Pests That Are Controlled or Suppressed by Pyramided 

1507xMON810 ..................................................................................................................214 

Table 3. Efficacy of Breeding Stacks Against First Generation European Corn Borer .................216 

Table 4. Efficacy of Breeding Stacks Against Fall Armyworm ......................................................217 

Table 5. Breeding Stacks Included in Categories 1, 2 and 3 ........................................................220 

Table 6. Proposed Total Acreage by State ....................................................................................221 

Table 7. Estimated Acreage per State/Territory for Nursery / Breeding Protocol ..........................222 

Table 8. Estimated Acreage per State/Territory for Yield / Agronomic Protocol ...........................224 

Table 9. Estimated Acreage per State/Territory for Efficacy / IRM Protocol .................................226 

Table 10. Estimated Acreage per State/Territory for Seed Production Protocol............................228 

Table 11. Estimated Acreage per State/Territory for Regulatory Protocol.....................................229 

Table 12. Summary of EUP Program Quantities for 2009 ............................................................231 

Figures 

Figure 1. Example of Master Label Weight Summary ...................................................................235 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 4 of 500
 

Transmittal Document

Submitter

Pioneer Hi-Bred International, Inc. 

2450 SE Oak Tree Court 

Ankeny, IA 50021 

Regulatory Action in Support of Which This Document is Submitted

Amended Application for an Experimental Use Permit with a Plant-Incorporated Protectant 

Pursuant to FIFRA §5(a): 

Amended Application for an Experimental Use Permit for 1507x59122xMON810, 

1507xMON810 and 59122XMON810 

29964-EUP-6 

Transmittal Date

October 30, 2009 

Submitter Reference Number

PHI-2008-139 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 5 of 500


EPA Forms 8570-4

Confidential Statement of Formula 

{See CBI Cross Reference Number 1} 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 8 of 500

 

EPA Form 8570-35

Data Matrix for 1507x59122xMON810 (see following pages) 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 10 of 500
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:1507x59122xMON810 

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Acute oral toxicity in mice: Cry1F Bacillus thuringiensis subsp. aizawai delta endotoxin 44691101 68467 PEEffectiveness data for Bacillus thuringiensis var. aizawai Cry1F insect control protein as expressed in maize 44691102 68467 PEBackground document on resistance management 44691103 68467 PEProduct characterization data for Bacillus thuringiensis var. aizawai: Cry1F control protein as expressed in maize 44714801 68467 PE

Characterization of gene inserts – Bacillus thuringiensis aizawai: Cry1F control protein as expressed in maize 44714802 68467 PEEquivalency of microbial and maize expressed Cry1F protein; characterization of test substances for biochemical and toxicological studies. In vitro digestibility of microbial and maize expressed Cry1F protein under simulated gastric conditions 44714803 68467 PEQuantitative ELISA analysis of Cry1F expression levels in maize MPS inbred and hybrid lines 1360, 1365, 1366 and 1369 (interim report) 44714804 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Comparison of the amino acid sequence similarity of Cry1F and PAT proteins to KnPER allergen protein 44971701 68467 PEMicrobial B.t. Cry1F (truncated) delta-endotoxin: Maize-insect-pest susceptibility study 45020101 68467 PECharacterization of inserted genes in Cry1F maize line 1507 45020102 68467 PECharacterization of expressed Cry1F protein in maize tissues (pollen, grain, grain-containing feed and purified maize-expressed Cry1F protein) and microbial expressed Cry1F delta-endotoxin by biological and biochemical procedures 45020103 68467 PE

Quantitative ELISA analysis of Cry1F and PAT expression levels in and compositional analysis of maize inbred and hybrid line 1362 and 1507 45020104 68467 PEEnvironmental fate of Cry1F protein incorporated into soil 45020105 68467 PE

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: an acute toxicity study with the earthworm in an artificial soil substrate 45020106 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Chronic exposure of Folsomia candida to bacterially expressed Cry1F protein 45020107 68467 PE

OECD 207 

B.t. Cry1F delta endotoxin: A 48-hour static-renewal acute toxicity test with the Cladoceran (Daphnia magna) using bacterially expressed B.t. Cry1F delta endotoxin, and pollen from maize expressing B.t.

Cry1F delta endotoxin 45020108 68467 PE

885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary study with green lacewing larvae 45020109 68467 PE

885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary study with the ladybird beetle 45020110 68467 PE

885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary toxicity study with parasitic hymenoptera 45020111 68467 PE

71-2 

Transgenic corn expressing Bacillus thuringiensis var. aizawai (B.t.) Cry1F delta endotoxin: A dietary toxicity study with Northern bobwhite 45020112 68467 PE

Field surveys of beneficial arthropods associated with Bacillus thuringiensis Cry1F maize 45020113 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Efficacy of Cry1F events TC1360 and TC1507 45020114 68467 PECry1F binding studies 45020115 68467 PE

Resistance management plan for transgenic maize expressing the Cry1F insecticidal protein from Bacillus thuringiensis var. aizawai 45020116 68467 PESupplement to MRID 44714801: Supplemental data - Product characterization data for Bacillus thuringiensis var. aizawai: Cry1F control protein as expressed in maize 45020117 68467 PESupplement to MRID 44691101: Supplemental data - Acute oral toxicity in mice: Bacillus thuringiensis 

var. aizawai: Cry1F delta endotoxin 45020118 68467 PE

Phosphinothricin acetyltransferase (PAT) protein: In vitro digestibility study 45041501 68467 PENon-target exposure and risk assessment for environmental dispersal of Cry1F maize pollen 45041502 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6- 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of the dietary effect(s) on honeybee development using bacterially expressed B.t. Cry1F 

delta endotoxin and pollen from maize expressing B.t. Cry1F delta endotoxin 45041503 68467 PE

Waiver request: Fish toxicity test with transgenic maize (corn) containing Bacillus thuringiensis var. aiaqoi (B.t.) Cry1F delta endotoxin 45044201 68467 PEHigh dose demonstration of Cry1F events TC1360 and TC1507: European corn borer 45131101 68467 PE

Toxicity of the Cry1F protein to neonate larvae of the monarch buttefly (Danaus plexippus (Linneaus)) 45131102 68467 PEPublic interest document for Cry1F-protected corn 45131103 29964 OW

Thermolability of Cry1F (truncated) delta endotoxin 45274801 68467 PECompositional analysis of maize MPS hybrid line 1507 45274802 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Cry1F lateral flow test kit procedure for analyzing Cry1F corn grain 45279301 68467 PEMethod validation report for the determination of Cry1F delta endotoxin protein in grain by EnzymeLinked Immunosorbent Assay 45279302 68467 PEPublic interest document for Cry1F protected corn 45298101 29964 OW

Supplement to MRID 45131102: Supplemental data - High dose demonstration of Cry1F events TC1360 and TC1507: European corn borer 45307701 68467 PEWaiver request: Fish toxicity test to assess the potential effects of maize containing Bacillus thuringiensis var. aizawai (Bt) Cry1F insecticidal protein (ICP) in native fish 45307702 68467 PE

Supplement to MRID 45020109: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with green lacewing larvae 45307801 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Supplement to MRID 45020110: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with green ladybird beetle 45307802 68467 PE

Supplement to MRID 45020111: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with parasitic hymenoptera 45307803 68467 PE

Supplement to MRID 45020106: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: An acute toxicity study with the earthworm in an artificial soil substrate 45307804 68467 PE

Supplement to MRID 45041503: Evaluation of the dietary effect(s) on honeybee development using bacterially expressed B.t. Cry1F delta endotoxin and pollen from maize expressing B.t. Cry1F delta endotoxin 45307805 68467 PESupplement to MRID 45298101: Supplemental data - Public interest document for Cry1F protected corn 45309401 29964 OW

Exposure and risk assessment of Herculex I Bt field corn pollen to Karner blue butterfly 45512901 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Nutritional equivalency of B.t. Cry1F maize - poultry 

feeding study 45622001 68467 PE

Field survey of beneficial arthropods associated with Bacillus thuringiensis Cry1F maize 45648001 68467 PE

Field surveys of non-target invertebrate populations in B.t. corn 45652001 68467 PEDevelopment and characterization of EnzymeLinked Immunosorbent Assay (ELISA) for detection of Cry1F protein 45685601 68467 PE

Independent laboratory validation of method GRM 02.13, determination of Cry1F delta endotoxin protein in corn grain by an of Enzyme-Linked Immunosorbent Assay 45685602 68467 PESupplement to MRID 45131102: Toxicity of the Cry1F protein to neonate larvae of the monarch butterfly (Danaus plexippus (Linneaus)) 45759701 68467 PEStewardship of Herculex I Insect Protection with respect to the secondary lepidopteran pest, western bean cutworm (Richia albicosta Smith) 45896601 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Lack of cross reactivity between Cry1F protein in Herculex I maize and the dust mite Der p7 protein with human sera positive for Der p7-IgE 46444001 68467 PEMonitoring the susceptibility of European corn borer to Cry1Ab and Cry1F Bt proteins: Results from the 2004 collections and diapausing larvae collected in 2003 46583101 80778 PE

Stewardship of event TC1507 maize with respect to the secondary lepidopteran pests lesser corn stalk borer (Elasmopalpus lignosellus Zeller), southern corn stalk borer (Diatraea crambidiodes Grote), and 

sugarcane borer (Biatraea saccharalis Fabricius) 46600201 68467 PE

Slide presentation summarizing European corn borer and Cry1F resistance monitoring update 46695801 68467 PE

Insect resistance management compliance assurance program report for corn borer-protected Bt corn 46747801 80778 PEField surveys of non-target invertebrate populations in Bt corn: Supplement to MRID No. 45652001 46784601 80778 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Monitoring the susceptibility of corn lepidopteran pests to Cry1Ab and Cry1F proteins: 2005 monitoring results 46874901 80778 PEResearch results on 2004 European corn borer collections from Hamilton County, Iowa: Cry1F 47011201 68467 PE

Insect resistance management compliance assurance program report for corn borer-protected Bt corn, corn rootworm-protected Bt corn, and corn borer/corn rootworm protected stacked Bt corn 47044401 80778 PEMonitoring the susceptibility of corn lepidopteran pests to Cry1Ab and Cry1F proteins: 2006 monitoring results 47118401 80778 PE

Soil accumulation of Cry1F protein after three years of cropping with Herculex I corn 47120701 68467 PETC1507 maize and fall armyworm in Puerto Rico 47176001 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product characterization data for Bacillus thuringiensis PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins expressed in transgenic maize plants 45242201 68467 PEEquivalency of microbial and maize-expressed PS149B1 proteins 45242203 68467 PEMicrobial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study 45242204 68467 PE

Comparison of the amino acid sequence of the Bacillus thuringiensis strain PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins to KnPER protein allergens 45242205 68467 PECharacterization of Pseudomonas produced and transgenic maize expressed phosphinothricin acetyltransferase (PAT) protein 45242206 68467 PE

PS149B1 14 kDa protein: Acute oral toxicity study in CD-1 mice 45242207 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

PS149B1 44 kDa protein: Acute oral toxicity study in CD-1 mice 45242208 68467 PEPS149B1 14 kDa and 44 kDa proteins: Acute oral toxicity study in CD-1 mice 45242209 68467 PEPS149B1 binary insecticidal crystal protein: A dietary toxicity study with ladybird beetle 45242210 68467 PE

The tri-trophic interaction between PS149B1 transformed maize, corn leaf aphid and ladybird beetle 45242211 68467 PEIn vitro digestibility of PS149B1 proteins 45242212 68467 PE

Quantitative ELISA analysis of PS149B1 protein expression levels in hybrid and inbred lines of maize 

event TC5639 (interim report) 45242213 68467 PE

Degradation of microbial binary PS149B1 delta endotoxin in a representative soil from the midWestern USA maize growing region 45242214 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product durability plan for transgenic maize expressing insecticidal crystal protein from Bacillus 

thuringiensis strain PS149B1 during the experimental use period 45242215 68467 PE

Field efficacy of PS149B1 maize events against corn rootworms 45242216 68467 PEMicrobial PS149B1 binary insecticidal crystal proteins, pollen expressing PS149B1 binary insecticidal crystal proteins, and individual PS149B1 14 kDa and 44 kDa insecticidal crystal proteins 45340701 68467 Pe

PS149B1binary insecticidal crystal protein: Acute toxicity study to the earthworm in an artificial substrate 45360201 68464 PELateral flow test kit method validation for the detection of the PS149B1 14 kDa and 44 kDa protein in maize grain 45383401 68467 PE

Heat lability of individual proteins of the PS149B1 binary ICP 45584501 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

In vitro simulated gastric fluid digestibility study of microbially derived Cry34Ab1 protein 45584502 68467 PE

Characterization of Cry34Ab1 and Cry35Ab1 from recombinant Pseudomonas flourescens and 

transgenic maize 45790401 68467 PE

Characterization of DNA inserted into transgenic corn events (Cry34Ab1 and Cry35Ab1) 45790402 68467 PEPS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout, Oncorhynchus mykiss Wallbaum 45790403 68467 PE

PS149B1 binary insecticidal crystal protein: Acute toxicity study with Daphnid, Daphnia magna Straus 45790404 68467 PEPS149B1 binary insecticidal crystal protein: Dietary toxicity to parasitic hymenoptera (Nasonia vitripennis) 45790405 68467 PE

Assessment of the chronic toxicity of diet containing Bacillus thuringiensis PS149B1 insecticidal crystal protein to collembolan (Folsomia candida) 45790406 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6- 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

PS149B1 binary insecticidal crystal protein: Dietary toxicity to green lacewing (Chrysoperla carnea) 45790407 68467 PESDS-PAGE sensitivity analysis for Cry35Ab1 in support of the simulated gastric fluid digestibility study MRID 45242212 45790408 68467 PETrait durability and experimental use of transgenic maize expressing the insecticidal crystalline proteins 

Cry34Ab1and Cry35Ab1 45790509 68467 PE

Field efficacy of Cry34Ab1/Cry35Ab1 maize events against corn rootworms 45790410 68467 PEProduct characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17658) 45790501 68467 PE

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 45790601 68467 PESummary heat lability studies with Cry34Ab1/Cry35Ab1 45808601 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Quantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17658 45833101 68467 PEQuantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17662 45833102 68467 PESlide presentation summarizing Cry34Ab1/Cry35Ab1 heat inactivation studies 45860201 68467 PEProbe MOA studies to assess potential for protein synthesis inhibition by Bacillus thuringiensis

PS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbit reticulocyte assay: Re-examination of lab notebook data 45942801 68467 PE

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 46030001 68467 PEPublic interest document for Cry34/35Ab1 corn rootworm-protected corn 46122701 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964- 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Independent laboratory validation Pioneer Hi-Bred International, Inc. ELISA method for the quantification of Cry34Ab1 protein from transgenic plants 46123901 68467 PEIndependent laboratory validation of Dow AgroSciences method GRM 03.13, “ Determination of Cry35Ab1 insecticidal crystal protein in maize tissue by Enzyme-Linked Immunosorbent Assay” 46123902 68467 PECry34/35 protein distribution and familiarity 46123903 68467 PEAgronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry34Ab1, cry35Ab1 and pat genes: Chile locations 46123904 68467 PE

Biological equivalency of Cry34/35Ab1 insecticidal crystal protein in transgenic plants derived from transgenic Pseudomonas flourscens 46123905 68467 PECharacterization of Cry34Ab and Cry35Ab1 proteins derived from transgenic maize event E4497.59.1.22 (DAS-59122-7) 46123906 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6- 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Characterization of phosphinothricin acetyltransferase (PAT) derived from transgenic maize event E4497.59.1.22 46123907 68467 PECharacterization of DNA inserted into transgenic corn events DAS-45216-6 and DAS-59122-7 46123908 68467 PEDetailed characterization of DNA into transgenic corn events DAS-45216-6 and DAS-59122-7 46123909 68467 PEEvaluation of microbe derived Cry34Ab1 and Cry35Ab1 proteins for protein synthesis inhibition activity 46123910 68467 PENutritional equivalency study of maize containing Cry34Ab1 and Cry35Ab1: Poultry feeding study 46123911 68467 PE

The effect of Cry34Ab1/Cry35Ab1 proteins on the development and mortality of the ladybird beetle Coleomegilla maculata DeGeer 46123912 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Non-target invertebrate ecological risk assessment for field corn expressing Cry34Ab1 and Cry35Ab1 insecticidal crystal proteins in event DAS-59122-7 46123913 68467 PEEvaluation of the impact corn rootworm control strategies on non-target arthropods 46123914 68467 PEInvestigations into dose of Cry34Ab1/Cry35Ab1 rootworm-resistant maize event DAS-59122-7 against western and northern corn rootworms in support of trait durability plans 46123915 68467 PEEffect on western corn rootworm adults feeding on Cry34/35Ab1 corn rootworm-protected corn tissue and implication for product durability 46123916 68467 PE

Evaluation of endangered/threatened insect species relative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids 46123917 68467 PETrait durability plan for Cry34/35 corn rootworm protected corn event DAS-59122-7 following commercialization 46123918 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Simulations of corn rootworm adaptation to Cry34/35 corn rootworm protected corn in support of trait durability plans for event DAS-59122-7 46123919 68467 PEDigestion of allergenic and non-allergenic proteins in simulated gastric fluid 46123920 68467 PEDigestion efficiency of allergens and non-allergens in simulated gastric fluid: Bacillus thuringiensis

Cry34/35Ab1 construct PHP17662 46388601 68467 PE

Lack of Cry34Ab1/Cry35Ab1 co-association in solution 46556801 68467 PEEvaluation of the sequence similarities of the 

Cry34Ab1, Cry35Ab1 and PAT proteins to the public protein sequence datasets 46584701 68467 PE

Summary report of a carabid beetle laboratory toxicity study using Cry34Ab1 and Cry35Ab1 including copies of references 46714101 68467 PEPreliminary resistance monitoring plan for Cry34/35Ab1 corn event DAS-59122-7 46769201 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Thermolability of PS149B1 binary delta endotoxin 45358401 68467 PESummary information in support of the Section 3 registration of Herculex XTRA Insect Protection corn 46297101 68467 PECharacterization of a stacked hybrid of transgenic corn lines DAS-01507-1 and DAS-59122-7 by comparison to the individual lines 46297102 68467 PEAgronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry1F, cry34Ab1, cry35Ab1 and pat genes: US and Canada locations 46297103 68467 PEAmended report:: Agronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry1F, cry34Ab1, cry35Ab1 and pat genes: US and Canada locations 46343801 68467 PENutritional equivalency study of stacked hybrid of transgenic maize line 1507 (event DAS-01507-1) and 59122 (event DAS-59122-7): Poultry feeding study 46343803 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6- 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Comparison of insect efficacy and expression in Cry34/35 corn events DAS-59122-7 and DAS59132-8 expressed as a single and a stack with DAS-01507-1 46343804 68467 PEField efficacy of Herculex XTRA (DAS-01507-1xDAS-59122-7) insect resistant corn 46343805 68467 PEInvestigation of potential interaction between Cry1F and the binary Cry34Ab1/Cry35Ab1 proteins 46343806 68467 PEThe effect of maize pollen expressing the Cry1F and Cry34Ab1/Cry35Ab1 proteins on the development and mortality of the ladybird beetle Coleomegilla maculata DeGeer 46343807 68467 PELaboratory bioassay assessment of corn pollen protein (Cry34/35 and Cry1F, stacked transgenes), and their effect on the larvae of monarch butterfly 46343808 68467 PEEquimolar ratio of Cry1F (tr) + Cry35Ab1 +Cry34Ab1: Acute oral toxicity study in CD-1 mice 46384301 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Detailed resistance monitoring plan for Cry34/35Ab1 corn event DAS-59122-7 47334201 68467 PEProposed revisions to IRM-related registration requirements for Cry1 Plant Incorporated Protectants in field corn 47407001 80778 PEProduct Chemistry: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994501 524 PE

Toxicology Summary: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994502 524 PE

Effectiveness Data: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994503 524 PE

Assessment of the in vitro digestive fate of Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein 43439201 524 PEEvaluation of the dietary effects of purified B.t.k. endotoxin proteins on honeybee larvae 43439202 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of the dietary effects of purified B.t.k. endotoxin proteins on honeybee larvae 43439202 524 PEEvaluation of the dietary effects of purified B.t.k. endotoxin proteins on honeybee adults 43439203 524 PEAcute oral toxicity study of Btk HD-1 tryptic core protein in albino mice 43468001 524 PEStability of the Cry1Ab insecticidal protein of Bacillus 

thuringiensis var. kurstaki (B.t.k. HD-1) in sucrose 

and honey solutions under non-refrigerated temperature conditions 43468002 524 PE

Activated Btk HD-1 protein: A dietary toxicity study with green lacewing larvae 43468003 524 PEActivated Btk HD-1 protein: A dietary toxicity study with parasitic hymenoptera (Brachymeria intermedia) 43468004 524 PEActivated Btk HD-1 protein: A dietary toxicity study with ladybird beetles 43468005 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Compositional comparison of Bacillus thuringiensissubsp. kurstaki HD-1 protein produced in ECB resistant corn and the commercial product, DIPEL® 43533203 524 PEAssessment of the equivalence of the Bacillus thuringiensis subsp. kurstaki HD-1 protein produced in Escherichia coli and European corn 

borer resistant corn 43533204 524 PE

A dietary toxicity study with MON80187 meal in the northern bobwhite 43533205 524 PEMolecular characterization of insect protected corn line MON810 43665501 524 PEEvaluation of insect protected corn lines in 1994 U.S. field test locations 43665502 524 PEAssessment of the equivalence of B.t.k. HD-1 protein produced in several insect protected corn lines and Escherichia coli 43665503 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of the European corn borer resistance corn line MON801 as a feed ingredient for catfish 43887901 524 PECry1Ab insecticidal protein: an acute toxicity study with the earthworm in an artificial soil substrate 43887902 524 PEEffect of the Bacillus thuringiensis insecticidal proteins Cry1Ab, Cry1Ac, CryII and CryIIIA on 

Folsomia candida, and Xenylla grisea (Insecta: 

collembola) 43941601 524 PESupplemental submission on the tissue expression and corn earworm (Helicoverpa zea) efficacy of the Cry1Ab protein in insect protected corn 44168601 524 PEChronic exposure of Folsomia candida to corn tissue 

expressing Cry1Ab protein 44271501 524 PE

Public interest document for plant pesticides Bacillus thuringiensis subsp. kurstaki insect control protein as expressed in corn 44288901 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964- 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Submission of efficacy data in support of registration of MON810 Yielgard® 446432002 524 PEBacillus thuringiensis subsp. kurstaki HD-1 insecticidal protein (B.t.k. HD-1 protein) shares no significant sequence similarity with proteins associated with allergy or celiac disease 45384901 524 PEValidated method for extraction and direct ELISA analysis of Cry1Ab protein in corn grain 45415401 524 PEPotential risk to endangered lepidoptera from Yieldgard corn pollen (Bt corn events MON810 and Bt11) with emphasison the Karner blue butterfly 45512201 524 PEComparison of broiler performance when fed diets containing Yieldgard® corn, Yieldgard® x Roundup®

Ready parental line or commercial corn hybrid 45611801 524 PE

Cry1Ab protein levels in leaf, pollen, whole plant, forage, root and grain tissues from corn event MON810 produced in U.S. field trials in 2002 45878901 524 PE

Signature Name and Title 

 Jamie Staley  Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Assessment of the potential for persistence and accumulation of Cry1Ab protein in soil as a result of sustained Bt corn use 46022401 524 PEEffects on monarch butterfly larvae after continuous exposure to Cry1Ab-expressing corn pollen during anthesis 46162001 524 PEPetition for exemption from the requirement of a tolerance for Bacillus thuringiensis Cry1, Cry2, and Cry3 classes of proteins and the genetic material necessary for the production of these proteins in or on all raw agricultural commodities when used as plant-pesticide active ingredients PP 7F4888 524 PEEvaluation of the potential effects of pollen from maize plants containing the events 1507, 59122 and MON810 to larvae of the Monarch butterfly, Danaus plexippus. Lepidoptera, Danaidae 47510902 29964 OWExpressed trait protein concentration of maize lines containing events DAS-Ø15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 and combined trait product DASØ15Ø7-1xMON-ØØ81Ø-6, DAS-59122-7x MONØØ81Ø-6 and DAS-Ø15Ø7-1xDAS-59122-7xMONØØ81Ø-6; Greenhouse 47510904 29964` OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Durability plan during the EUP phase for MON810 crossed with events 1507, 59122 and 1507x59122 47510901 29964 OWLiterature references for Experimental Use Permit: 1507xMON810, 1507x59122xMON810 and 59122xMON810 47510905 29964 OWMolecular characterization of combined trait product 1507x59122xMON810xNK603 using Southern blot analysis and event-specific polymerase chain reaction 47677801 29964 OWLaboratory characterization of key lepidopteran response to pyramided events 47677802 29964 OWField efficacy, toxin/allergen homology, and trait durability supplemental information for 1507x59122xMON810, 1507xMON810, and 59122xMON810 47677803 29964 OWLiterature references supporting the application for Registration of 1507x59122xMON810 47677804 29964 OW

Signature Name and Title 

 Jamie Staley  Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Comparison of the amino acid sequence identity between the Cry1F protein and known protein allergens 47786401 29964 OWComparison of the amino acid sequence identity between the Cry34Ab1 and Cry35Ab1 proteins and known protein allergens 47786402 29964 OWComparison of the amino acid sequence identity between the PAT protein and known protein allergens 47786403 29964 OWComparison of the amino acid sequence identity between the Cry1Ab protein from event MON810 and known protein allergens 47786404 29964 OWEvaluation of the amino acid sequence similarity of the Cry1F protein to the NCBI protein sequence datasets 47786405 29964 OWEvaluation of the amino acid sequence similarity of the Cry1Ab protein from event MON810 to the NCBI protein sequence datasets 47786406 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of the amino acid sequence similarity of the PAT protein to the NCBI protein sequence datasets 47786407 29964 OWEvaluation of the amino acid sequence similarity of the Cry34Ab1 and Cry35Ab1 proteins to the NCBI protein sequence datasets 47786408 29964 OWAdditional addendum to expressed trait protein concentration of maize lines containing events DASØ15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 and 

combined trait product DAS-Ø15Ø7-1xMONØØ81Ø-6, DAS-59122-7x MON-ØØ81Ø-6 and DASØ15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6; Greenhouse Submitted 10/8/09 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6- 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE29964 OW68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE29964 OW68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964- 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE29964 OW68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE80778 PE68467 PE68467 PE80778 PE80778 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

80778 PE68467 PE80778 PE80778 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE80778 PE

524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE

29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

1507x59122xMON81

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
 

EPA Form 8570-35

Data Matrix for 1507xMON810 (see following pages) 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 73 of 500
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline Study Name MRID Number Submitter Sta

Acute oral toxicity in mice: Cry1F Bacillus thuringiensis subsp. aizawai delta endotoxin 44691101 68467 PEEffectiveness data for Bacillus thuringiensis var. aizawai Cry1F insect control protein as expressed in maize 44691102 68467 PEBackground document on resistance management 44691103 68467 PEProduct characterization data for Bacillus thuringiensis var. aizawai: Cry1F control protein as expressed in maize 44714801 68467 PE

Characterization of gene inserts – Bacillus thuringiensis aizawai: Cry1F control protein as expressed in maize 44714802 68467 PEEquivalency of microbial and maize expressed Cry1F protein; characterization of test substances for biochemical and toxicological studies. In vitro digestibility of microbial and maize expressed Cry1F protein under simulated gastric conditions 44714803 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Quantitative ELISA analysis of Cry1F expression levels in maize MPS inbred and hybrid lines 1360, 1365, 1366 and 1369 (interim report) 44714804 68467 PEComparison of the amino acid sequence similarity of Cry1F and PAT proteins to KnPER allergen protein 44971701 68467 PEMicrobial B.t. Cry1F (truncated) delta-endotoxin: Maize-insect-pest susceptibility study 45020101 68467 PECharacterization of inserted genes in Cry1F maize line 1507 45020102 68467 PECharacterization of expressed Cry1F protein in maize tissues (pollen, grain, grain-containing feed and purified maize-expressed Cry1F protein) and microbial expressed Cry1F delta-endotoxin by biological and biochemical procedures 45020103 68467 PE

Quantitative ELISA analysis of Cry1F and PAT expression levels in and compositional analysis of maize inbred and hybrid line 1362 and 1507 45020104 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Environmental fate of Cry1F protein incorporated into soil 45020105 68467 PECry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: an acute toxicity study with the earthworm in an artificial soil substrate 45020106 68467 PE

Chronic exposure of Folsomia candida to bacterially expressed Cry1F protein 45020107 68467 PE

OECD 207 

B.t. Cry1F delta endotoxin: A 48-hour static-renewal acute toxicity test with the Cladoceran (Daphnia magna) using bacterially expressed B.t. Cry1F delta endotoxin, and pollen from maize expressing B.t.

Cry1F delta endotoxin 45020108 68467 PE

885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary study with green lacewing larvae 45020109 68467 PE

885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary study with the ladybird beetle 45020110 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta885.4340 

Cry1F Bacillus thuringiensis var. aizawai delta 

endotoxin: A dietary toxicity study with parasitic hymenoptera 45020111 68467 PE

71-2 

Transgenic corn expressing Bacillus thuringiensis var. aizawai (B.t.) Cry1F delta endotoxin: A dietary toxicity study with Northern bobwhite 45020112 68467 PE

Field surveys of beneficial arthropods associated with Bacillus thuringiensis Cry1F maize 45020113 68467 PE

Efficacy of Cry1F events TC1360 and TC1507 45020114 68467 PECry1F binding studies 45020115 68467 PEResistance management plan for transgenic maize expressing the Cry1F insecticidal protein from Bacillus thuringiensis var. aizawai 45020116 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: August 12 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Supplement to MRID 44714801: Supplemental data - Product characterization data for Bacillus thuringiensis var. aizawai: Cry1F control protein as expressed in maize 45020117 68467 PESupplement to MRID 44691101: Supplemental data - Acute oral toxicity in mice: Bacillus thuringiensis var. aizawai: Cry1F delta endotoxin 45020118 68467 PE

Phosphinothricin acetyltransferase (PAT) protein: In vitro digestibility study 45041501 68467 PENon-target exposure and risk assessment for environmental dispersal of Cry1F maize pollen 45041502 68467 PEEvaluation of the dietary effect(s) on honeybee development using bacterially expressed B.t. Cry1F 

delta endotoxin and pollen from maize expressing B.t. Cry1F delta endotoxin 45041503 68467 PE

Waiver request: Fish toxicity test with transgenic maize (corn) containing Bacillus thuringiensis var. aiaqoi (B.t.) Cry1F delta endotoxin 45044201 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

High dose demonstration of Cry1F events TC1360 and TC1507: European corn borer 45131101 68467 PE

Toxicity of the Cry1F protein to neonate larvae of the monarch buttefly (Danaus plexippus (Linneaus)) 45131102 68467 PEPublic interest document for Cry1F-protected corn 45131103 29964 OW

Thermolability of Cry1F (truncated) delta endotoxin 45274801 68467 PECompositional analysis of maize MPS hybrid line 1507 45274802 68467 PECry1F lateral flow test kit procedure for analyzing Cry1F corn grain 45279301 68467 PEMethod validation report for the determination of Cry1F delta endotoxin protein in grain by EnzymeLinked Immunosorbent Assay 45279302 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Public interest document for Cry1F protected corn 45298101 29964 OWSupplement to MRID 45131102: Supplemental data - High dose demonstration of Cry1F events TC1360 and TC1507: European corn borer 45307701 68467 PEWaiver request: Fish toxicity test to assess the potential effects of maize containing Bacillus thuringiensis var. aizawai (Bt) Cry1F insecticidal protein (ICP) in native fish 45307702 68467 PE

Supplement to MRID 45020109: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with green lacewing larvae 45307801 68467 PE

Supplement to MRID 45020110: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with green ladybird beetle 45307802 68467 PE

Supplement to MRID 45020111: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: A dietary toxicity study with parasitic hymenoptera 45307803 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Supplement to MRID 45020106: Cry1F Bacillus

thuringiensis var. aizawai delta endotoxin: An acute toxicity study with the earthworm in an artificial soil substrate 45307804 68467 PE

Supplement to MRID 45041503: Evaluation of the dietary effect(s) on honeybee development using bacterially expressed B.t. Cry1F delta endotoxin and pollen from maize expressing B.t. Cry1F delta endotoxin 45307805 68467 PESupplement to MRID 45298101: Supplemental data - Public interest document for Cry1F protected corn 45309401 29964 OW

Exposure and risk assessment of Herculex I Bt field corn pollen to Karner blue butterfly 45512901 68467 PENutritional equivalency of B.t. Cry1F maize - poultry 

feeding study 45622001 68467 PE

Field survey of beneficial arthropods associated with Bacillus thuringiensis Cry1F maize 45648001 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Field surveys of non-target invertebrate populations in B.t. corn 45652001 68467 PEDevelopment and characterization of EnzymeLinked Immunosorbent Assay (ELISA) for detection of Cry1F protein 45685601 68467 PE

Independent laboratory validation of method GRM 02.13, determination of Cry1F delta endotoxin protein in corn grain by an of Enzyme-Linked Immunosorbent Assay 45685602 68467 PESupplement to MRID 45131102: Toxicity of the Cry1F protein to neonate larvae of the monarch butterfly (Danaus plexippus (Linneaus)) 45759701 68467 PE

Stewardship of Herculex I Insect Protection with respect to the secondary lepidopteran pest, western bean cutworm (Richia albicosta Smith) 45896601 29964 OWLack of cross reactivity between Cry1F protein in Herculex I maize and the dust mite Der p7 protein with human sera positive for Der p7-IgE 46444001 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Monitoring the susceptibility of European corn borer to Cry1Ab and Cry1F Bt proteins: Results from the 2004 collections and diapausing larvae collected in 2003 46583101 80778 PEStewardship of event TC1507 maize with respect to the secondary lepidopteran pests lesser corn stalk borer (Elasmopalpus lignosellus Zeller), southern corn stalk borer (Diatraea crambidiodes Grote), and sugarcane borer (Biatraea saccharalis Fabricius) 46600201 68467 PE

Slide presentation summarizing European corn borer and Cry1F resistance monitoring update 46695801 68467 PEInsect resistance management compliance assurance program report for corn borer-protected Bt corn 46747801 80778 PE

Field surveys of non-target invertebrate populations in Bt corn: Supplement to MRID No. 45652001 46784601 80778 PEMonitoring the susceptibility of corn lepidopteran pests to Cry1Ab and Cry1F proteins: 2005 monitoring results 46874901 80778 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Research results on 2004 European corn borer collections from Hamilton County, Iowa: Cry1F 47011201 68467 PEInsect resistance management compliance assurance program report for corn borer-protected Bt corn, corn rootworm-protected Bt corn, and corn borer/corn rootworm protected stacked Bt corn 47044401 80778 PE

Monitoring the susceptibility of corn lepidopteran pests to Cry1Ab and Cry1F proteins: 2006 monitoring results 47118401 80778 PESoil accumulation of Cry1F after three years of cropping with Herculex 1 corn 47120701 68467 PE

TC1507 maize and fall armyworm in Puerto Rico 47176001 68467 PEProduct Chemistry: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994501 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Toxicology Summary: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994502 524 PE

Effectiveness Data: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994503 524 PE

Assessment of the in vitro digestive fate of Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein 43439201 524 PEEvaluation of the dietary effects of purified B.t.k. endotoxin proteins on honeybee larvae 43439202 524 PE

Evaluation of the dietary effects of purified B.t.k. endotoxin proteins on honeybee adults 43439203 524 PEAcute oral toxicity study of Btk HD-1 tryptic core protein in albino mice 43468001 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Stability of the Cry1Ab insecticidal protein of Bacillus 

thuringiensis var. kurstaki (B.t.k. HD-1) in sucrose 

and honey solutions under non-refrigerated temperature conditions 43468002 524 PE

Activated Btk HD-1 protein: A dietary toxicity study with green lacewing larvae 43468003 524 PEActivated Btk HD-1 protein: A dietary toxicity study with parasitic hymenoptera (Brachymeria intermedia) 43468004 524 PEActivated Btk HD-1 protein: A dietary toxicity study with ladybird beetles 43468005 524 PECompositional comparison of Bacillus thuringiensissubsp. kurstaki HD-1 protein produced in ECB resistant corn and the commercial product, DIPEL® 43533203 524 PE

Assessment of the equivalence of the Bacillus thuringiensis subsp. kurstaki HD-1 protein produced in Escherichia coli and European corn 

borer resistant corn 43533204 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

A dietary toxicity study with MON80187 meal in the northern bobwhite 43533205 524 PEMolecular characterization of insect protected corn line MON810 43665501 524 PEEvaluation of insect protected corn lines in 1994 U.S. field test locations 43665502 524 PEAssessment of the equivalence of B.t.k. HD-1 protein produced in several insect protected corn lines and Escherichia coli 43665503 524 PEEvaluation of the European corn borer resistance corn line MON801 as a feed ingredient for catfish 43887901 524 PECry1Ab insecticidal protein: an acute toxicity study with the earthworm in an artificial soil substrate 43887902 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Effect of the Bacillus thuringiensis insecticidal proteins Cry1Ab, Cry1Ac, CryII and CryIIIA on 

Folsomia candida, and Xenylla grisea (Insecta: 

collembola) 43941601 524 PESupplemental submission on the tissue expression and corn earworm (Helicoverpa zea) efficacy of the Cry1Ab protein in insect protected corn 44168601 524 PEChronic exposure of Folsomia candida to corn tissue 

expressing Cry1Ab protein 44271501 524 PE

Public interest document for plant pesticides Bacillus thuringiensis subsp. kurstaki insect control protein as expressed in corn 44288901 524 PESubmission of efficacy data in support of registration of MON810 Yielgard® 446432002 524 PEBacillus thuringiensis subsp. kurstaki HD-1 insecticidal protein (B.t.k. HD-1 protein) shares no significant sequence similarity with proteins associated with allergy or celiac disease 45384901 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Validated method for extraction and direct ELISA analysis of Cry1Ab protein in corn grain 45415401 524 PE

Potential risk to endangered lepidoptera from Yieldgard corn pollen (Bt corn events MON810 and Bt11) with emphasison the Karner blue butterfly 45512201 524 PEComparison of broiler performance when fed diets containing Yieldgard® corn, Yieldgard® x Roundup®

Ready parental line or commercial corn hybrid 45611801 524 PE

Cry1Ab protein levels in leaf, pollen, whole plant, forage, root and grain tissues from corn event MON810 produced in U.S. field trials in 2002 45878901 524 PEBioinformatics evaluation of the Cry1Ab protein produced in corn event MON810 utilizing an allergen database 45878902 524 PEAssessment of the potential for persistence and accumulation of Cry1Ab protein in soil as a result of sustained Bt corn use 46022401 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Effects on monarch butterfly larvae after continuous exposure to Cry1Ab-expressing corn pollen during anthesis 46162001 524 PE

Petition for exemption from the requirement of a tolerance for Bacillus thuringiensis Cry1, Cry2, and Cry3 classes of proteins and the genetic material necessary for the production of these proteins in or on all raw agricultural commodities when used as plant-pesticide active ingredients PP 7F4888 524 PEEvaluation of the potential effects of pollen from maize plants containing the events 1507 and MON810 to larvae of the Monarch butterfly, Danaus plexippus. Lepidoptera, Danaidae 47510903 29964 OWExpressed trait protein concentration of maize lines containing events DAS-Ø15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 and combined trait product DASØ15Ø7-1xMON-ØØ81Ø-6, DAS-59122-7x MONØØ81Ø-6 and DAS-Ø15Ø7-1xDAS-59122-7xMONØØ81Ø-6; Greenhouse 47510904 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product: 1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Durability plan during the EUP phase for MON810 crossed with events 1507, 59122 and 1507x59122 47510901 29964 OWLiterature references for Experimental Use Permit: 1507xMON810, 1507x59122xMON810 and 59122xMON810 47510905 29964 OWMolecular characterization of corn combined trait product 1507x59122xMON810xNK603 corn using Southern blot analysis and Polymerase Chain Reaction 47677801 29964 OWLaboratory characterization of key lepidopteran response to pyramided events 47677802 29964 OWField efficacy, toxin/allergen homology, and trait durability supplemental information for 1507x59122xMON810, 1507xMON810, and 59122xMON810 4768803 29964 OWLiterature references supporting the application for registration of 1507x59122xMON810 4768804 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Comparison of the amino acid sequence identity between the Cry1F protein and known protein allergens 47786401 29964 OWComparison of the amino acid sequence identity between the Cry34Ab1 and Cry35Ab1 proteins and known protein allergens 47786402 29964 OWComparison of the amino acid sequence identity between the PAT protein and known protein allergens 47786403 29964 OWComparison of the amino acid sequence identity between the Cry1Ab protein from event MON810 and known protein allergens 47786404 29964 OWEvaluation of the amino acid sequence similarity of the Cry1F protein to the NCBI protein sequence datasets 47786405 29964 OWEvaluation of the amino acid sequence similarity of the Cry1Ab protein from event MON810 to the NCBI protein sequence datasets 47786406 29964 OW

Signature Name and Title 

Jamie Staley U.S. Registration Manager 

Date October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of the amino acid sequence similarity of the PAT protein to the NCBI protein sequence datasets 47786407 29964 OWEvaluation of the amino acid sequence similarity of the Cry34Ab1 and Cry35Ab1 proteins to the NCBI protein sequence datasets 47786408 29964 OWAdditional addendum to expressed trait protein concentration of maize lines containing events DASØ15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 and 

combined trait product DAS-Ø15Ø7-1xMONØØ81Ø-6, DAS-59122-7x MON-ØØ81Ø-6 and DASØ15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6; Greenhouse Submitted 10/8/09 29964 OW

Signature Name and Title 

Jamie Staley U.S. Registration Manager 

Date October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021 

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 7250 NW 62nd Avenue, Johnston, IA 50131-0552

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE29964 OW68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE29964 OW68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE29964 OW68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

80778 PE68467 PE68467 PE80778 PE80778 PE80778 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE80778 PE80778 PE68467 PE68467 PE

524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964- 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 7250 NW 62nd Avenue, Johnston, IA 50131-0552

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE

29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021

Product:

1507xMON810 

Ingredient Bacillus thuringiensis Cry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
 

EPA Form 8570-35

Data Matrix for 59122xMON810 (see following pages) 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 114 of 500
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product characterization data for Bacillus thuringiensis PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins expressed in transgenic maize plants 45242201 68467 PEEquivalency of microbial and maize-expressed PS149B1 proteins 45242203 68467 PEMicrobial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study 45242204 68467 PE

Comparison of the amino acid sequence of the Bacillus thuringiensis strain PS149B1 13.6 kDa and 43.8 kDa insecticidal crystal proteins to KnPER protein allergens 45242205 68467 PECharacterization of Pseudomonas produced and transgenic maize expressed phosphinothricin acetyltransferase (PAT) protein 45242206 68467 PE

PS149B1 14 kDa protein: Acute oral toxicity study in CD-1 mice 45242207 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

PS149B1 44 kDa protein: Acute oral toxicity study in CD-1 mice 45242208 68467 PEPS149B1 14 kDa and 44 kDa proteins: Acute oral toxicity study in CD-1 mice 45242209 68467 PEPS149B1 binary insecticidal crystal protein: A dietary toxicity study with ladybird beetle 45242210 68467 PEThe tri-trophic interaction between PS149B1 transformed maize, corn leaf aphid and ladybird beetle 45242211 68467 PE

In vitro digestibility of PS149B1 proteins 45242212 68467 PEQuantitative ELISA analysis of PS149B1 protein expression levels in hybrid and inbred lines of maize event TC5639 (interim report) 45242213 68467 PE

Degradation of microbial binary PS149B1 delta endotoxin in a representative soil from the midWestern USA maize growing region 45242214 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product durability plan for transgenic maize expressing insecticidal crystal protein from Bacillus 

thuringiensis strain PS149B1 during the experimental use period 45242215 68467 PE

Field efficacy of PS149B1 maize events against corn rootworms 45242216 68467 PEMicrobial PS149B1 binary insecticidal crystal proteins, pollen expressing PS149B1 binary insecticidal crystal proteins, and individual PS149B1 14 kDa and 44 kDa insecticidal crystal proteins 45340701 68467 Pe

PS149B1binary insecticidal crystal protein: Acute toxicity study to the earthworm in an artificial substrate 45360201 68464 PELateral flow test kit method validation for the detection of the PS149B1 14 kDa and 44 kDa protein in maize grain 45383401 68467 PE

Heat lability of individual proteins of the PS149B1 binary ICP 45584501 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

In vitro simulated gastric fluid digestibility study of microbially derived Cry34Ab1 protein 45584502 68467 PECharacterization of Cry34Ab1 and Cry35Ab1 from recombinant Pseudomonas flourescens and 

transgenic maize 45790401 68467 PE

Characterization of DNA inserted into transgenic corn events (Cry34Ab1 and Cry35Ab1) 45790402 68467 PE

PS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout, Oncorhynchus mykiss Wallbaum 45790403 68467 PEPS149B1 binary insecticidal crystal protein: Acute toxicity study with Daphnid, Daphnia magna Straus 45790404 68467 PE

PS149B1 binary insecticidal crystal protein: Dietary toxicity to parasitic hymenoptera (Nasonia vitripennis) 45790405 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Assessment of the chronic toxicity of diet containing Bacillus thuringiensis PS149B1 insecticidal crystal protein to collembolan (Folsomia candida) 45790406 68467 PEPS149B1 binary insecticidal crystal protein: Dietary toxicity to green lacewing (Chrysoperla carnea) 45790407 68467 PESDS-PAGE sensitivity analysis for Cry35Ab1 in support of the simulated gastric fluid digestibility study MRID 45242212 45790408 68467 PE

Trait durability and experimental use of transgenic maize expressing the insecticidal crystalline proteins Cry34Ab1and Cry35Ab1 45790509 68467 PEField efficacy of Cry34Ab1/Cry35Ab1 maize events against corn rootworms 45790410 68467 PE

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17658) 45790501 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 45790601 68467 PESummary heat lability studies with Cry34Ab1/Cry35Ab1 45808601 68467 PEQuantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17658 45833101 68467 PEQuantitative ELISA analysis of Cry34Ab1 and Cry35Ab1 proteins expressed in maize plants transformed with the vector PHP17662 45833102 68467 PE

Slide presentation summarizing Cry34Ab1/Cry35Ab1 heat inactivation studies 45860201 68467 PEProbe MOA studies to assess potential for protein synthesis inhibition by Bacillus thuringiensis

PS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbit reticulocyte assay: Re-examination of lab notebook data 45942801 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Product characterization data for Bacillus thuringiensis Cry34Ab1 and Cry35Ab1 proteins expressed in transgenic maize plants (PHP17662) 46030001 68467 PEPublic interest document for Cry34/35Ab1 corn rootworm-protected corn 46122701 29964 OWIndependent laboratory validation Pioneer Hi-Bred International, Inc. ELISA method for the quantification of Cry34Ab1 protein from transgenic plants 46123901 68467 PEIndependent laboratory validation of Dow AgroSciences method GRM 03.13, “ Determination of Cry35Ab1 insecticidal crystal protein in maize tissue by Enzyme-Linked Immunosorbent Assay” 46123902 68467 PE

Cry34/35 protein distribution and familiarity 46123903 68467 PEAgronomic characteristics, quantitative ELISA and nutrient composition analysis of hybrid maize lines containing cry34Ab1, cry35Ab1 and pat genes: Chile locations 46123904 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Biological equivalency of Cry34/35Ab1 insecticidal crystal protein in transgenic plants derived from transgenic Pseudomonas flourscens 46123905 68467 PECharacterization of Cry34Ab and Cry35Ab1 proteins derived from transgenic maize event E4497.59.1.22 (DAS-59122-7) 46123906 68467 PECharacterization of phosphinothricin acetyltransferase (PAT) derived from transgenic maize event E4497.59.1.22 46123907 68467 PECharacterization of DNA inserted into transgenic corn events DAS-45216-6 and DAS-59122-7 46123908 68467 PE

Detailed characterization of DNA into transgenic corn events DAS-45216-6 and DAS-59122-7 46123909 68467 PEEvaluation of microbe derived Cry34Ab1 and Cry35Ab1 proteins for protein synthesis inhibition activity 46123910 68467 PE

Nutritional equivalency study of maize containing Cry34Ab1 and Cry35Ab1: Poultry feeding study 46123911 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

The effect of Cry34Ab1/Cry35Ab1 proteins on the development and mortality of the ladybird beetle Coleomegilla maculata DeGeer 46123912 68467 PENon-target invertebrate ecological risk assessment for field corn expressing Cry34Ab1 and Cry35Ab1 insecticidal crystal proteins in event DAS-59122-7 46123913 68467 PEEvaluation of the impact corn rootworm control strategies on non-target arthropods 46123914 68467 PE

Investigations into dose of Cry34Ab1/Cry35Ab1 rootworm-resistant maize event DAS-59122-7 against western and northern corn rootworms in support of trait durability plans 46123915 68467 PEEffect on western corn rootworm adults feeding on Cry34/35Ab1 corn rootworm-protected corn tissue and implication for product durability 46123916 68467 PE

Evaluation of endangered/threatened insect species relative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids 46123917 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Trait durability plan for Cry34/35 corn rootworm protected corn event DAS-59122-7 following commercialization 46123918 68467 PESimulations of corn rootworm adaptation to Cry34/35 corn rootworm protected corn in support of trait durability plans for event DAS-59122-7 46123919 68467 PEDigestion of allergenic and non-allergenic proteins in simulated gastric fluid 46123920 68467 PEDigestion efficiency of allergens and non-allergens in simulated gastric fluid: Bacillus thuringiensis

Cry34/35Ab1 construct PHP17662 46388601 68467 PE

Lack of Cry34Ab1/Cry35Ab1 co-association in solution 46556801 68467 PEEvaluation of the sequence similarities of the Cry34Ab1, Cry35Ab1 and PAT proteins to the public protein sequence datasets 46584701 68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Summary report of a carabid beetle laboratory toxicity study using Cry34Ab1 and Cry35Ab1 including copies of references 46714101 68467 PEPreliminary resistance monitoring plan for Cry34/35Ab1 corn event DAS-59122-7 46769201 68467 PEThermolability of PS149B1 binary delta endotoxin 45358401 68467 PEProduct Chemistry: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994501 524 PE

Toxicology Summary: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994502 524 PE

Effectiveness Data: Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein as expressed in corn 

plants 42994503 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Assessment of the in vitro digestive fate of Bacillus thuringiensis subsp. kurstaki (B.t.k.) HD-1 protein 43439201 524 PEEvaluation of the dietary effects of purified B.t.k. endotoxin proteins on honeybee larvae 43439202 524 PEEvaluation of the dietary effects of purified B.t.k. endotoxin proteins on honeybee adults 43439203 524 PEAcute oral toxicity study of Btk HD-1 tryptic core protein in albino mice 43468001 524 PEStability of the Cry1Ab insecticidal protein of Bacillus 

thuringiensis var. kurstaki (B.t.k. HD-1) in sucrose 

and honey solutions under non-refrigerated temperature conditions 43468002 524 PE

Activated Btk HD-1 protein: A dietary toxicity study with green lacewing larvae 43468003 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Activated Btk HD-1 protein: A dietary toxicity study with parasitic hymenoptera (Brachymeria intermedia) 43468004 524 PEActivated Btk HD-1 protein: A dietary toxicity study with ladybird beetles 43468005 524 PECompositional comparison of Bacillus thuringiensissubsp. kurstaki HD-1 protein produced in ECB resistant corn and the commercial product, DIPEL® 43533203 524 PEAssessment of the equivalence of the Bacillus thuringiensis subsp. kurstaki HD-1 protein produced in Escherichia coli and European corn 

borer resistant corn 43533204 524 PE

A dietary toxicity study with MON80187 meal in the northern bobwhite 43533205 524 PEMolecular characterization of insect protected corn line MON810 43665501 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of insect protected corn lines in 1994 U.S. field test locations 43665502 524 PEAssessment of the equivalence of B.t.k. HD-1 protein produced in several insect protected corn lines and Escherichia coli 43665503 524 PEEvaluation of the European corn borer resistance corn line MON801 as a feed ingredient for catfish 43887901 524 PECry1Ab insecticidal protein: an acute toxicity study with the earthworm in an artificial soil substrate 43887902 524 PEEffect of the Bacillus thuringiensis insecticidal proteins Cry1Ab, Cry1Ac, CryII and CryIIIA on 

Folsomia candida, and Xenylla grisea (Insecta: 

collembola) 43941601 524 PESupplemental submission on the tissue expression and corn earworm (Helicoverpa zea) efficacy of the Cry1Ab protein in insect protected corn 44168601 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 corn 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn. Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Chronic exposure of Folsomia candida to corn tissue 

expressing Cry1Ab protein 44271501 524 PE

Public interest document for plant pesticides Bacillus thuringiensis subsp. kurstaki insect control protein as expressed in corn 44288901 524 PESubmission of efficacy data in support of registration of MON810 Yielgard® 44643202 524 PEBacillus thuringiensis subsp. kurstaki HD-1 insecticidal protein (B.t.k. HD-1 protein) shares no significant sequence similarity with proteins associated with allergy or celiac disease 45384901 524 PEValidated method for extraction and direct ELISA analysis of Cry1Ab protein in corn grain 45415401 524 PEPotential risk to endangered lepidoptera from Yieldgard corn pollen (Bt corn events MON810 and Bt11) with emphasison the Karner blue butterfly 45512201 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Comparison of broiler performance when fed diets containing Yieldgard® corn, Yieldgard® x Roundup®

Ready parental line or commercial corn hybrid 45611801 524 PE

Cry1Ab protein levels in leaf, pollen, whole plant, forage, root and grain tissues from corn event MON810 produced in U.S. field trials in 2002 45878901 524 PEBioinformatics evaluation of the Cry1Ab protein produced in corn event MON810 utilizing an allergen database 45878902 524 PEAssessment of the potential for persistence and accumulation of Cry1Ab protein in soil as a result of sustained Bt corn use 46022401 524 PEEffects on monarch butterfly larvae after continuous exposure to Cry1Ab-expressing corn pollen during anthesis 46162001 524 PEPetition for exemption from the requirement of a tolerance for Bacillus thuringiensis Cry1, Cry2, and Cry3 classes of proteins and the genetic material necessary for the production of these proteins in or on all raw agricultural commodities when used as plant-pesticide active ingredients PP 7F4888 524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Evaluation of the potential effects of pollen from maize plants containing the events 1507, 59122 and MON810 to larvae of the Monarch butterfly, Danaus plexippus. Lepidoptera, Danaidae 47510902 29964 OWExpressed trait protein concentration of maize lines containing events DAS-Ø15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 and combined trait product DASØ15Ø7-1xMON-ØØ81Ø-6, DAS-59122-7x MONØØ81Ø-6 and DAS-Ø15Ø7-1xDAS-59122-7xMONØØ81Ø-6; Greenhouse 47510904 29964 OWDurability plan during the EUP phase for MON810 crossed with events 1507, 59122 and 1507x59122 47510901 29964 OWLiterature references for Experimental Use Permit: 1507xMON810, 1507x59122xMON810 and 59122xMON810 47510905 29964 OWMolecular characterization of corn combined trait product 1507x59122xMON810xNK603 corn using Southern blot analysis and Polymerase Chain Reaction 47677801 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Laboratory characterization of key lepidopteran response to pyramided events 47677802 29964 OWField efficacy, toxin/allergen homology, and trait durability supplemental information for 1507x59122xMON810, 1507xMON810, and 59122xMON810 4768803 29964 OWLiterature references supporting the application for Registration of 1507x59122xMON810 47677804 29964 OWComparison of the amino acid sequence identity between the Cry1F protein and known protein allergens 47786401 29964 OWComparison of the amino acid sequence identity between the Cry34Ab1 and Cry35Ab1 proteins and known protein allergens 47786402 29964 OWComparison of the amino acid sequence identity between the PAT protein and known protein allergens 47786403 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Comparison of the amino acid sequence identity between the Cry1Ab protein from event MON810 and known protein allergens 47786404 29964 OWEvaluation of the amino acid sequence similarity of the Cry1F protein to the NCBI protein sequence datasets 47786405 29964 OWEvaluation of the amino acid sequence similarity of the Cry1Ab protein from event MON810 to the NCBI protein sequence datasets 47786406 29964 OWEvaluation of the amino acid sequence similarity of the PAT protein to the NCBI protein sequence datasets 47786407 29964 OWEvaluation of the amino acid sequence similarity of the Cry34Ab1 and Cry35Ab1 proteins to the NCBI protein sequence datasets 47786408 29964 OWAddendum to expressed trait protein concentration of maize lines containing events DAS-Ø15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 and combined trait product DAS-Ø15Ø7-1xMON-ØØ81Ø-6, DAS59122-7x MON-ØØ81Ø-6 and DAS-Ø15Ø7-1xDAS59122-7xMON-ØØ81Ø-6; Greenhouse Submitted 9/18/09 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address:  Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021 

Product: 59122xMON810 

Ingredient Bacillus thuringiensis Cry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

Additional addendum to expressed trait protein concentration of maize lines containing events DASØ15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 and 

combined trait product DAS-Ø15Ø7-1xMONØØ81Ø-6, DAS-59122-7x MON-ØØ81Ø-6 and DASØ15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6; Greenhouse Submitted 10/8/09 29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6- 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE29964 OW68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE68467 PE68467 PE68467 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

68467 PE68467 PE68467 PE

524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

524 PE524 PE524 PE524 PE524 PE524 PE

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW29964 OW29964 OW29964 OW29964 OW29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Form 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY 

1200 Pennsylvania Avenue, N.W. 

Washington, D.C. 20460

Paperwork Reduction Act Notice: The public reporting burden for this collection of information is estimated to average 0.25 hours per response for registration aresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regardinother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Env1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.

DATA MATRIX

Date: October 30, 2009 EPA Reg. No. / File Symb

29964-EUP-6 

Applicant's/Registrant's Name & Address: 

Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021

Product:

59122xMON810 

Ingredient Bacillus thuringiensis Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn Guideline Reference Number Guideline Study Name MRID Number Submitter Sta

29964 OW

Signature Name and Title 

 Jamie Staley  U.S. Registration Manager 

Date 

October 3

EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
 

Sections A Through G of the EUP Application

Section A. Product Chemistry 

Pioneer is developing two new products that pyramid Lepidopteran insecticidal corn traits. These 

products are being made by crossing registered events using conventional breeding. The 1507xMON180 

product expresses the Cry1F and Cry1Ab Bt-derived protein, each highly selective and effective in 

controlling certain economically important lepidopteran insect pests of field corn. For markets where 

control of corn rootworm is necessary, Pioneer is developing the 1507x59122xMON810 product by 

adding (also by conventional breeding) a third registered event that expresses the Cry34/35Ab1 protein, 

active against corn rootworm larvae. The 59122xMON810 breeding stack is not intended as a 

commercial product, but rather will be used as a breeding intermediate and/or inbred for product 

development of the 1507x59122xMON810 breeding stack. 

Combining Cry1F and Cry1Ab brings together two distinct modes of action against key lepidopteran 

pests. Research has shown that Cry1F and Cry1Ab do not share any high-affinity binding sites (Perreia 

thesis). Pyramiding the 1507 and MON810 events will reduce the risk of resistance in insects controlled 

by both events. 

Enclosed is an application for an Experimental Use Permit (EUP) for field testing of insect-resistant corn. 

The application is for new (non-registered) combinations of previously registered events: DAS-Ø15Ø7-1 

(also known as 1507), DAS-59122-7 (also known as 59122), and MON-ØØ81Ø-6 (also known as 

MON810). These combinations are as follows: 

1) DAS-Ø15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6 (1507x59122xMON810) 

2) DAS-Ø15Ø7-1xMON-ØØ81Ø-6 (1507xMON810) 

3) DAS-59122-7xMON-ØØ81Ø-6 (59122xMON810) 

1507 corn expresses the Cry1F protein that is insecticidally active against certain lepidopteran pests of 

corn. MON810 expresses the Cry1Ab protein that is likewise active against certain lepidopteran pests of 

corn. In a pyramided breeding combination, these two events express two Cry proteins for enhanced 

pest spectrum and insect resistant management durability. Also included in the EUP is a triple breeding 

stack that includes the 59122 event, which expresses the Cry34/35Ab1 protein for control of corn 

rootworm pests. The 59122xMON810 breeding stack is not intended as a commercial product, but rather 

will be used as a breeding intermediate and/or inbred for product development of the 

1507x59122xMON810 breeding stack. 

Table 1 specifies the registered events that comprise the combinations that are the subject of the EUP: 

Table 1. Cross-reference Event Table 

OECD Event 

Name 

Event Name 

Abbreviation Product Name Registration Number PIP Expressed 

DAS-Ø15Ø7-1 1507 Herculex1 I 29964-3 Cry1F 

DAS-59122-7 59122 Herculex 

Rootworm 29964-4 Cry34/35 

MON-ØØ81Ø-6 MON810 YieldGard2

 Corn 

Borer 524-489 Cry1Ab 

 

1

 YieldGard® is a registered trademark used under license from MONsanto Company. 

2

 Herculex® I Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred. Herculex is a registered 

trademarks of Dow AgroSciences LLC.

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 155 of 500
 

Section B. Proposed Labels 

The following pages contain the proposed Master Label for the EUP, including labels for the EUP Test 

Plants (1-3), as well as labels for Registered PIPs (4-13) and Non-registered PIPs (14-44) that will be 

planted in EUP experiments. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 156 of 500
 

Master Label 

29964-EUP-6 

Subparts: 

1) 1507 x 59122 x MON810 Corn Seed 

2) 1507 x MON810 Corn Seed 

3) 59122 x MON810 Corn Seed 

4) 1507 Corn Seed 

5) 59122 Corn Seed 

6) MON810 Corn Seed 

7) 1507 x 59122 Corn Seed 

8) Bt11 Corn Seed 

9) MIR604 Corn Seed 

10) MIR162 Corn Seed 

11) Bt11 x MIR604 Corn Seed 

12) Bt11 x MIR162 Corn Seed 

13) Bt11 x MIR162 x MIR604 Corn Seed 

14) 1507 x 59122 x MON810 x MIR162 Corn Seed 

15) 1507 x 59122 x MON810 x MIR604 Corn Seed 

16) 1507 x 59122 x MIR162 x MIR604 Corn Seed 

17) 1507 x MON810 x MIR162 x MIR604 Corn Seed 

18) 59122 x MON810 x MIR162 x MIR604 Corn Seed 

19) 1507 x 59122 x Bt11 x MIR162 x MIR604 Corn Seed 

20) 1507 x 59122 x Bt11 x MIR162 Corn Seed 

21) 1507 x 59122 x Bt11 x MIR604 Corn Seed 

22) 1507 x Bt11 x MIR162 x MIR604 Corn Seed 

23) 59122 x Bt11 x MIR162 x MIR604 Corn Seed 

24) 1507 x Bt11 x MIR162 Corn Seed 

25) 1507 x Bt11 x MIR604 Corn Seed 

26) 59122 x Bt11 x MIR162 Corn Seed 

27) 59122 x Bt11 x MIR604 Corn Seed 

28) 1507 x 59122 x MIR162 Corn Seed 

29) 1507 x 59122 x MIR604 Corn Seed 

30) 1507 x MON810 x MIR162 Corn Seed 

31) 1507 x MON810 x MIR604 Corn Seed 

32) 1507 x MIR162 x MIR604 Corn Seed 

33) 59122 x MON810 x MIR162 Corn Seed 

34) 59122 x MON810 x MIR604 Corn Seed 

35) 59122 x MIR162 x MIR604 Corn Seed 

36) MON810 x MIR162 x MIR604 Corn Seed 

37) 1507 x Bt11 Corn Seed 

38) 1507 x MIR162 Corn Seed 

39) 1507 x MIR604 Corn Seed 

40) 59122 x MIR162 Corn Seed 

41) 59122 x MIR604 Corn Seed 

42) MON810 x MIR162 Corn Seed 

43) MON810 x MIR604 Corn Seed 

44) MIR162 x MIR604 Corn Seed 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 157 of 500
 

1) 1507 x 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, and Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.00036%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0036%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.......................................................................<0.00023%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn MON-ØØ81Ø-6 ...........................................................................<0.000033%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn .....................................................................................................<0.000016%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 158 of 500
 

2) 1507 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1F and Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .......................................................................<0.0003%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn MON-ØØ81Ø-6 ...........................................................................<0.000036%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 159 of 500
 

3) 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0045%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.......................................................................<0.00023%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn MON-ØØ81Ø-6 ...........................................................................<0.000039%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 160 of 500
 

4) 1507 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1F. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...........................................................................................................<0.002%* 

* Maximum percent (wt/wt) of total extractable protein in grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 29964-3 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 161 of 500
 

5) 59122 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain corn rootworm pests: Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 29964-4 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 501313 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened coleopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 162 of 500
 

6) MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn MON-ØØ81Ø-6 ...............................................................................<0.0070%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 524-489 

EPA Establishment Number: 524-MO-002 Contact Person: Tracy Rood 

Monsanto Company Phone: (515) 270-4036 

700 Chesterfield Parkway North. Email: tracy.rood@pioneer.com

St. Louis, Missouri 63198 

NET CONTENTS: _______________ pounds of corn seed 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 163 of 500
 

7) 1507 x 59122 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.00038%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.......................................................................<0.00633%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.......................................................................<0.00034%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00004%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 29964-5 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 164 of 500
 

8) Bt11 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .......................................................................<0.0016%* 

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn .....................................................................................................<0.000004%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 67979-1 

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood 

Syngenta Seeds Inc. Phone: (515) 270-4036 

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd. 

Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 165 of 500
 

9) MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against corn rootworm pests: mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO.: 67979-5 

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood 

Syngenta Seeds Inc. Phone: (515) 270-4036 

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd. 

Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened coleopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 166 of 500
 

10) MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredient:

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO: 67979-14 

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood 

Syngenta Seeds Inc. Phone: (515) 270-4036 

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd. 

Research Triangle Park, NC 27709 

NET CONTENTS: ___________ pounds of corn seed 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 167 of 500
 

11) Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .....................................................................<0.00019%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00008%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn .....................................................................................................<0.000004%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00004%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO: 67979-8 

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood 

Syngenta Seeds Inc. Phone: (515) 270-4036 

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd. 

Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 168 of 500
 

12) Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .......................................................................<0.0011%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .........................................................................<0.0102%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn .....................................................................................................<0.000041%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .....................................................................................................<0.000041%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO: 67979-12 

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood 

Syngenta Seeds Inc. Phone: (515) 270-4036 

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd. 

Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 169 of 500
 

13) Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .....................................................................<0.00025%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .........................................................................<0.0165%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ....................................................................<0.000077%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00003%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00065%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA REG NO: 67979-13 

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood 

Syngenta Seeds Inc. Phone: (515) 270-4036 

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd. 

Research Triangle Park, NC 27709 

NET CONTENTS: _______________ pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 170 of 500
 

14) 1507 x 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7................................................................... <0.000161%** 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 ................................................................... <0.0070%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ..................................................................................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 171 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 172 of 500
 

15) 1507 x 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7................................................................... <0.000161%** 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 ................................................................... <0.0070%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 173 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 174 of 500
 

16) 1507 x 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7................................................................... <0.000161%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 175 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 176 of 500
 

17) 1507 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 ................................................................... <0.0070%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...........................................................................................................<0.002%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 177 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 178 of 500
 

18) 59122 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 .....................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 179 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 180 of 500
 

19) 1507 x 59122 x Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7................................................................... <0.000161%** 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 ..................................................................... <0.0016%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................. <0.0000069%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 181 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 182 of 500
 

20) 1507 x 59122 x Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7................................................................... <0.000161%** 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 ..................................................................... <0.0016%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................. <0.0000069%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ..................................................................................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 183 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 184 of 500
 

21) 1507 x 59122 x Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7................................................................... <0.000161%** 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .......................................................................<0.0016%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................. <0.0000069%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 185 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 186 of 500
 

22) 1507 x Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 ..................................................................... <0.0016%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................... <0.000004%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 187 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 188 of 500
 

23) 59122 x Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .......................................................................<0.0016%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 189 of 500
 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 190 of 500
 

24) 1507 x Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 ..................................................................... <0.0016%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................... <0.000004%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ..................................................................................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 191 of 500
 

25) 1507 x Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 ..................................................................... <0.0016%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................... <0.000004%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 192 of 500
 

26) 59122 x Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .......................................................................<0.0016%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Inert Ingredients

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 193 of 500
 

27) 59122 x Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 .......................................................................<0.0016%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 194 of 500
 

28) 1507 x 59122 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................. <0.0000069%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ..................................................................................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 195 of 500
 

29) 1507 x 59122 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1 and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7....................................................................... <0.0117%** 

 

Bacillus thuringiensis Cry35b1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7................................................................... <0.000161%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................. <0.0000069%** 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 196 of 500
 

30) 1507 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1F, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 ................................................................... <0.0070%** 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...........................................................................................................<0.002%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ..................................................................................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 197 of 500
 

31) 1507 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 ................................................................... <0.0070%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...........................................................................................................<0.002%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 198 of 500
 

32) 1507 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...........................................................................................................<0.002%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) of dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 199 of 500
 

33) 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 .....................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 200 of 500
 

34) 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 .....................................................................<0.0070%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 201 of 500
 

35) 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 202 of 500
 

36) MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 .....................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 203 of 500
 

37) 1507 x Bt11 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1F and Cry1Ab. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502) 

necessary for its production in corn event SYN-BTØ11-1 ..................................................................... <0.0016%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ................................................................................................... <0.000004%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 204 of 500
 

38) 1507 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1F and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 ..................................................................... <0.00403%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ..........................................................................................................< 0.002%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ..................................................................................................... <0.00019%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue 

Email: tracy.rood@pioneer.com

Johnston, IA 50131NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 205 of 500
 

39) 1507 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1F and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999) 

necessary for its production in corn event DAS-Ø15Ø7-1 .....................................................................<0.01415%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 .................................................................... <0.00313%** 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...........................................................................................................<0.002%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn ....................................................................................................... <0.0006%** 

* Maximum percent (wt/wt) of total extractable protein in grain 

** Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 206 of 500
 

40) 59122 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 207 of 500
 

41) 59122 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against corn rootworm pests: Cry34/35Ab1 and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.........................................................................<0.0117%* 

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662) 

necessary for its production in corn event DAS-59122-7.....................................................................<0.000161%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material 

necessary for its production in corn ...................................................................................................<0.0000069%* 

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened coleopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 208 of 500
 

42) MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran pests: Cry1Ab and VIP3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 .....................................................................<0.0070%* 

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .......................................................................................................<0.00019%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran 

species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 209 of 500
 

43) MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07) 

necessary for its production in corn event MON-ØØ81Ø-6 .....................................................................<0.0070%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 210 of 500
 

44) MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6 

This package contains seed for transgenic insect-resistant corn that produces the following proteins with activity 

against certain lepidopteran and corn rootworm pests: VIP3A and mCry3A. 

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use 

Program. For use only at an application site of a Participant or Coordinator and in accordance with the terms and 

conditions of the Experimental Use Permit (EUP). This labeling must be in possession of the user at the time the 

corn seed is planted. 

Active Ingredients:

Bacillus thuringiensis Vip3Aa20 protein and the genetic material (pNOV1300) 

necessary for its production in corn event SYN-IR162-4 .......................................................................<0.00403%* 

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26) 

necessary for its production in corn event SYN-IR6Ø4-5 ......................................................................<0.00313%* 

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material 

necessary for its production in corn .........................................................................................................<0.0006%* 

* Maximum percent (wt/wt) in dry grain 

CAUTION 

Keep Out of Reach of Children 

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood 

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036 

7300 NW 62nd Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131 

NET CONTENTS: ___________pounds of corn seed. 

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may 

only be used according to the field protocols as specified in the approved EUP program. Participants and 

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds. 

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran and 

coleopteran species as listed by the US Fish and Wildlife Service. 

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminate water, food or feed by storage or disposal. Any seed 

not used in these experiments must be returned to the seed provider or disposed of as specified in the field 

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before 

destroying. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 211 of 500
 

Section C. Reference to Available Environmental Toxicity Data 

Summary of data for individual events 

59122, 1507, and MON 810 are each registered events; extensive data have been submitted to EPA 

and have been previously reviewed (U.S. EPA, 2001 & 2005). To summarize, nontarget organism 

testing was performed on each of the single events (or their respective Bt proteins) with honeybees 

(larvae and adults), Daphnia magna, lady beetles, parasitic hymenoptera, green lacewing, 

earthworms, and collembolans. No adverse effects were found at environmentally relevant exposures 

for any of the species tested. As one of the most publicly recognized insects, monarch butterfly larvae 

were also tested for sensitivity to Cry1F and Cry1Ab, and no significant risk to monarchs was found at 

environmentally relevant exposures. Additionally, environmental fate data indicate that Cry1F, 

Cry34/35Ab1 and Cry1Ab dissipate rapidly from soil. Therefore accumulation of the proteins in soil 

should be minimal, thereby decreasing the likelihood of exposure to nontarget soil organisms. The 

Agency previously concluded that the levels of Cry 1F, Cry 1Ab, and Cry 34/35Ab1 likely to occur in 

the environment will not pose unreasonable risk to nontarget organisms, including mammals, birds, 

fish, bees, and other invertebrates, based on the required and voluntary data submitted. 

Specific data for proposed stack events 

Pioneer measured the concentrations of Cry1F, Cry34/35Ab1, Cry1Ab and PAT in plant tissues of the 

proposed stacked events. Expression levels were similar to those of the single events (PHI-2008-

006), thereby indicating no increase in potential exposure concentrations compared to the previously 

reviewed individual events. 

Pioneer also evaluated the effects of pollen from the proposed stacked events on monarch butterfly 

larvae. No adverse effects were seen (PHI-2008-104 and PHI-2008-105); therefore, we do not expect 

any additional risk from the stacked products compared to single events for non-target lepidoptera.

In addition, Pioneer confirmed that the efficacy of 1507 pyramided with MON810 is additive in nature 

(as opposed to synergistic or antagonistic) against European corn borer, southwestern corn borer, 

corn earworm, and fall armyworm (PHI-2008-014). Because there was no evidence of synergy during 

testing of several key target pests, we do not expect synergy to occur in non-target insects. 

Based on the lack of environmental and mammalian effects in the individual events, the similarity in 

expression levels, the lack of synergy seen in additivity testing, and the low likelihood of exposure, we 

expect no adverse effects from the proposed stacked products to mammals, birds, fish, bees, other 

invertebrates, and plants. Lack of exposure due to the small scale of testing plots and the limited 

duration of the EUP also points to a very low likelihood of risk from the proposed stacked product, 

similar to the findings by EPA for the previously registered individual events. In addition, the EUP 

Program will be conducted on land that is currently in agricultural use. Therefore, we do not expect 

that the proposed stacked product, which may include all of these proteins, will have a different 

environmental profile than the individual products that have been previously reviewed by EPA. 

References 

U.S. EPA. 2001. Environmental Assessment: Bt Plant-Incorporated Protectants. Biopesticides 

Registration Action Document. October 15, 2001. Accessed June 24, 2008: 

http://www.epa.gov/oppbppd1/biopesticides/pips/bt_brad2/3-ecological.pdf

U.S. EPA. 2005. Environmental Assessment: Bacillus thuringiensis Cry34/35Ab1 Corn Biopesticide 

Registration Action Document. October 2005. Accessed June 24, 2008: 

http://www.epa.gov/pesticides/biopesticides/ingredients/tech_docs/brad_006490.pdf

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 212 of 500
 

Section D. Residue Data 

The Cry1F, Cry1Ab and Cry34/35 proteins and the genetic material necessary for their production in 

corn are plant-incorporated protectants (PIPs). These PIPs are not applied to the corn crop as 

conventional pesticides would be, but rather are synthesized by plants carrying the cry1f, Cry1Ab and 

cry34/35 genes. Residue data per se are not available for a PIP. However, for purposes of assessing 

dietary and environmental risk of 1507x59122xMON810, 1507xMON810 and 59122XMON810, the 

concentrations of Cry1F, Cry1(A)b, Cry34/35 and phosphinothricin acetyltransferase (PAT) proteins 

were determined in different plant tissues at different plant growth stages using quantitative ELISA 

assays. The results from this determination are summarized in Volume 5 of this submission (PHI2008-006). 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 213 of 500
 

Section E. Effectiveness Data 

To date, limited efficacy data have been generated on the EUP breeding stacks listed in Table 1. 

However, the individual events are well characterized with respect to efficacy, and 1507 and MON810 

have been demonstrated to be additive in nature, as opposed to synergistic or antagonistic (PHI-2008-

114). Therefore, the breeding pyramids of 1507xMON810 and 1507x59122xMON810 are expected to 

be efficacious against the pests listed in Table 2. 

Table 2. Lepidopteran Pests That Are Controlled or Suppressed by Pyramided 1507xMON810* 

Scientific name Common name 1507 

Activity 

MON810 

Activity 

Predicted 

1507 x MON810 

Activity 

Agrotis ipsilon Black cutworm Yes No Yes 

Helicoverpa zea Corn earworm Yes Yes Yes 

Ostrinia nubilalis European corn borer Yes Yes Yes 

Spodoptera frugiperda Fall armyworm Yes Yes Yes 

Diatraea grandiosella Southwestern corn 

borer 

Yes Yes Yes 

Richia albicosta Western bean cutworm Yes No Yes 

Elasmopalpus 

lignosellus 

Lesser corn stalk borer Yes No Yes 

Diatrae crambidoides Southern corn stalk 

borer 

Yes Yes Yes 

Diatrea saccharalis Sugarcane borer Yes No Yes 

Papaipema nebris Common stalk borer No Yes Yes 

* based on current labels 

The breeding stacks of 1507x59122xMON810 and 59122xMON810 are expected to be equal in 

efficacy to the 59122 event alone against corn rootworm pests: Diabrotica virgifera virgifera, western 

corn rootworm (WCRW), Diabrotica berberi, northern corn rootworm (NCRW), and Diabrotica 

virgiferea zeae, Mexican corn rootworm (MCRW). 

Under the proposed EUP program, efficacy against lepidopteran and coleopteran pests will be 

evaluated in a range of environments, growing conditions and genetic backgrounds. 

Thus far, limited field efficacy trials have been completed characterizing first generation European corn 

borer, Ostrinia nubilalis (Hubner) (ECB), foliar feeding and fall armyworm, Spodoptera frugiperda (J.E. 

Smith) (FAW), whorl feeding on three maize hybrids containing events 1507x59122, 

1507x59122xMON810, and a negative control (isoline). The experimental design included nine 

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Study Number: PHI-2008-139

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entries (each a single-row plot with 30” row spacing) arranged in a randomized complete block with 

three replications. 

For trials characterizing first generation ECB feeding, 100 neonates/plant were manually infested three 

times within a 7-day period (300 total neonates/plant) beginning when plants reached growth stage 

V5. Approximately three weeks after the last infestation, mean damage ratings were taken on either 

10 or 15 individual plants per plot, depending on location (Table 3). 

For trials characterizing fall armyworm feeding, 20 neonates/plant were manually infested three times 

within a 10-day period (60 total neonates/plant) beginning when plants reached growth stage V4. 

Approximately two weeks after the last infestation, mean damage ratings were taken on either 10 or 

15 plants per plot, depending on location (Table 4). 

Results to date indicate that early vegetative-stage maize hybrids containing either events 

1507x59122xMON810 or events 1507x59122 were nearly immune to first generation ECB and FAW 

foliar injury. Additionally, there were no significant differences in mean damage ratings between 

hybrids containing events 1507x59122xMON810 and hybrids with only 1507x59122 for both pests at 

all locations. 

These results demonstrate the efficacy of these events is stable regardless of stacking or pyramiding 

in the event combinations named in Table 1. The EUP will allow for more extensive efficacy testing to 

further characterize the pest spectrum and level of efficacy of named event combinations under a 

wider range of environmental conditions.

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Table 3. Efficacy of Breeding Stacks Against First Generation European Corn Borer 

Mean damage ratings characterizing first generation ECB foliar feeding on maize hybrids containing 

events 1507x59122, 1507x59122xMON810, or a negative control (isoline) at 5 field trial locations in 

2008. 

Location Event Combination na Mean Damage Rating 

Standard Error b,c

1507x59122 135 9.0  0.00 a 

York, NE 1507x59122xMON810 135 9.0  0.00 a 

Negative control 135 5.4  0.07 b 

1507x59122 135 9.0  0.00 a 

Dallas Center, IA 1507x59122xMON810 135 9.0  0.00 a 

Negative control 135 5.1  0.07 b 

1507x59122 90 9.0  0.00 a 

Johnston, IA 1507x59122xMON810 90 9.0  0.00 a 

Negative control 89 5.6  0.07b 

1507x59122 135 9.0  0.00 a 

Mankato, MN 1507x59122xMON810 135 9.0  0.00 a 

Negative control 135 5.8  0.09 b 

1507x59122 135 9.0  0.00 a 

Princeton, IL 1507x59122xMON810 132 9.0  0.00 a 

Negative control 135 6.0  0.08 b 

a

 n = number of plants characterized; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15 

plants/plot depending on location. 

b

 Damage ratings on individual plants were determined using the following visual rating scale: 

9. No visible leaf injury or a small amount of pin or fine shot-hole type injury on a few leaves. 

8. Small amount of shot-hole type lesions on a few leaves. 

7. Shot-hole injury common on several leaves. 

6. Several leaves with shot-hole and elongated lesions (Lesions <0.5” in length). 

5. Several leaves with elongated lesions (Lesions 0.5” to 1.0” in length). 

4. Several leaves with elongated lesions (Lesions >1.0” in length). 

3. Long lesions (>1.0”) common on about one-half the leaves. 

2. Long lesions (>1.0”) common on about two-thirds the leaves. 

1. Most of the leaves with long lesions. c

 Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD 

test, P > 0.05). 

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Study Number: PHI-2008-139

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Table 4. Efficacy of Breeding Stacks Against Fall Armyworm 

Mean damage ratings characterizing FAW foliar feeding on maize hybrids containing events 

1507x59122, 1507x59122xMON810, or a negative control (isoline) at 2 field trial locations in 2008. 

Location Event Combination na Mean Damage Rating 

Standard Errorb,c

1507x59122 135 8.9  0.02 a 

Dallas Center, IA 1507x59122xMON810 135 8.9  0.02 a 

Negative control 135 2.4  0.07 b 

1507x59122 90 9.0  0.00 a 

Johnston, IA 1507x59122xMON810 89 9.0  0.02 a 

Negative control 89 2.8  0.09 b 

a

 n = number of plants characterized; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15 

plants/plot depending on location. 

b

 Damage ratings on individual plants were determined using the following visual rating scale: 

9. No damage to pinhole lesions present on whorl leaves. 

8. Pinholes and small circular lesions present on whorl leaves. 

7. Small circular lesions and a few small elongated (rectangular shaped) lesions up to 0.5” in 

length present on whorl and furl leaves. 

6. Several small elongated lesions 0.5” to 1” in length on a few whorl and furl leaves. 

5. Several large elongated lesions greater than 1” in length present on a few whorl and furl leaves 

and/or a few small to mid-sized uniform to irregular shaped holes (basement membrane 

consumed) in whorl and furl leaves. 

4. Several large elongated lesions present on several whorl and furl leaves and/or several large 

uniform to irregular shaped holes in whorl and furl leaves. 

3. Many elongated lesions of all sizes present on several whorl leaves plus several large uniform 

to irregular shaped holes in whorl and furl leaves. 

2. Many elongated lesions of all sizes present on most whorl and furl leaves plus many mid to 

large-sized uniform to irregular shaped holes in whorl and furl leaves. 

1. Whorl and furl leaves almost totally destroyed. c

 Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD 

test, P > 0.05). 

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Section F. Tolerance if Testing Involves Use in Food or Feed 

Phosphinothricin acetyltransferase (PAT), the marker protein in 1507 and 59122 corn, is exempt from 

the requirement of a tolerance in all plants: 40CFR §174.522. 

MON810 does not contain a marker protein. 

Cry1F, the PIP in 1507 corn, is exempt from the requirement of a tolerance in corn: 40CFR §174.520. 

Cry34Ab1 and Cry35Ab1, the PIPs in 59122 corn, are exempt from the requirement of a tolerance in 

corn: 40CFR §174.506. 

Cry1Ab, the PIP in MON810 corn, is exempt from the requirement of a tolerance in all plants: 40CFR 

§174.511. 

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Study Number: PHI-2008-139

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Section G. Proposed Experimental Program 

For the adequate development of insect-protected transgenic plants, it is necessary to properly 

characterize the performance of the crop through large-scale field testing. In order to thoroughly 

evaluate corn plants, experiments must be grown at a large number of locations, with many 

replications of each experiment, in order to obtain sufficient data for hybrid evaluation and 

advancement. A total of 34,725 acres in 25 states and Puerto Rico is being requested for this EUP. 

This acreage is justified for the following reasons: 

 Placement of experiments with research centers and cooperators is typically not decided until 

early spring of any given year. Spring weather conditions can affect planting locations. 

Therefore, the proposed EUP acreage needs to take into account the possibility of experiments 

being planted in a variety of locations and states. Because of this need for flexibility, the 

proposed acreage is likely an overestimate of the amount of EUP material that will actually be 

planted. 

 A large number of potential combinations of registered events may be tested. As Table 5 

shows, in addition to the three breeding stacks that comprise the EUP test plants, there are 10 

individual registered events and registered breeding combinations (Category 2) that may be 

included in experiments for breeding or comparison purposes. Category 3 contains 31 different 

breeding combinations that are non-registered PIPs which could be planted in EUP 

experiments. All together there are 44 different events and combinations that could be tested 

under this EUP. Even allocating a small number of estimated acres per protocol and/or state for 

each of the 44 entries increases the total acreage request. In fact, ~72% of the acreage request 

is to account for potential PIP comparators from Categories 2 and 3. In addition, ~1% of the 

acreage request is for border rows and non-PIP entries for breeding or comparison purposes 

 There are a large number of target pests for the EUP test plants (Section G3.)—three corn 

rootworm pests and ten lepidopteran pests. In order to conduct adequate testing for these 

pests, some of which occur sporadically, many locations in many states are needed. 

 As the EUP test plants advance toward commercialization, an increasing number of acres are 

needed to conduct inbred and hybrid seed production for future product development and 

testing. Large numbers of seed production acres are needed to evaluate the costs of producing 

hybrid seed, the phenotypic characteristics of male and female inbreds in different 

environments, the yield of different inbred combinations in different environments, etc. 

G1. Participants in the Experimental Program 

See CBI Cross Reference Number 2 

G2. Acreage and Seed Quantities by State or Territory for the EUP Program 

Pioneer has endeavored to forecast its acreage needs and field testing program for 2010. The quantity of 

acres being requested in this application is required for product development and evaluation. As 

recommended in EPA’s “Tips for Plant-Incorporated Protectant (PIP) Experimental Use Permit (EUP) 

Program Submission”, this acreage calculation includes the following categories: 

 Category 1: EUP Test Plants; 

 Category 2: Registered PIPs for breeding or comparison purposes; 

 Category 3: Non-registered PIPs; 

 Category 4: Registered PIPs (other than those listed in Category #2) for comparison purposes 

only (i.e., “commercial checks”); 

 Non-PIP plants (for example, herbicide tolerant or non-transgenic); and 

 Border rows, if used (not required). 

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Study Number: PHI-2008-139

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Table 5 lists the breeding stacks included in Categories 1, 2 and 3. Each of the individual events 

listed is registered by EPA and all PIP proteins have full tolerance exemptions. 

Table 5. Breeding Stacks Included in Categories 1, 2 and 3 

Category 1 

EUP Test Plants 

Category 2 

Registered PIPs for Breeding 

or Comparison Purposes 

Category 3 

Non-registered PIPs 

1507 x 59122 x MON810 1507 1507 x 59122 x MON810 x MIR162 

1507 x MON810 59122 1507 x 59122 x MON810 x MIR604 

59122 x MON810 MON810 1507 x 59122 x MIR162 x MIR604 

1507 x 59122 1507 x MON810 x MIR162 x MIR604 

Bt11 59122 x MON810 x MIR162 x MIR604 

MIR604 1507 x 59122 x Bt11 x MIR162 x MIR604 

MIR162 1507 x 59122 x Bt11 x MIR162

Bt11 x MIR604 1507 x 59122 x Bt11 x MIR604

Bt11 x MIR162 1507 x Bt11 x MIR162 x MIR604

Bt11 x MIR162 x MIR604 59122 x Bt11 x MIR162 x MIR604

1507 x Bt11 x MIR162 

1507 x Bt11 x MIR604

59122 x Bt11 x MIR162 

59122 x Bt11 x MIR604 

1507 x 59122 x MIR162 

1507 x 59122 x MIR604 

1507 x MON810 x MIR162 

1507 x MON810 x MIR604 

1507 x MIR162 x MIR604 

59122 x MON810 x MIR162 

591122 x MON810 x MIR604 

59122 x MIR162 x MIR604 

MON810 x MIR162 x MIR604 

1507 x Bt11 

1507 x MIR162 

1507 x MIR604 

 59122 x MIR162 

 59122 x MIR604 

 MON810 x MIR162 

 MON810 x MIR604 

 MIR162 x MIR604 

Many of the breeding stacks listed in Category 3 will likely not be planted to any appreciable extent. 

However, in order to enable full flexibility in the breeding and testing program, many potential 

combinations are listed. 

Table 6 provides the maximum acreage per state, and Table 7-11 provides the maximum acreage per 

protocol. The allocation of these acres between traits and protocols (Tables 7-11) represents best 

estimates as of this date. Weather events, pest infestation levels, unforeseen regulatory data 

demands from other countries, and other factors, may necessitate a reallocation of acres between 

protocols and traits. Table 12 summarizes the amount of seed planted and the total estimated amount 

of active ingredient to be used in the EUP Program. 

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Study Number: PHI-2008-139

Page 220 of 500
Table 6. Proposed Total Acreage by State

State PIP

PIPs for 

Comparison 

Only* Non-PIP Border Total

AR 509.5 4 9 18 540.5

CA 494 14 8 28 544

CO 1236 6 6 16 1264

GA 387 5 8 17 417

HI 649 1 106 8 764

IA 4347.5 31 124 73 4575.5

IL 4085.5 16 115 39 4255.5

IN 1312.5 14 11 31 1368.5

KS 1223.5 3 5 13 1244.5

LA 30.5 0 1 2 33.5

MI 2842 4 108 17 2971

MN 1645.5 6 10 22 1683.5

MO 680.5 5 9 19 713.5

MS 363 2 3 6 374

NC 389.5 4 4 15 412.5

NE 3345.5 15 115 38 3513.5

OK 30.5 0 1 2 33.5

OR 2530 0 100 0 2630

PA 773 4 4 12 793

PR 145 0 5 5 155

SD 672.5 4 5 14 695.5

TN 686 5 5 15 711

TX 1127.5 5 105 18 1255.5

WA 2490 0 100 0 2590

WI 1161.5 6 4 15 1186.5

Total 33157 154 971 443 34725

* This acreage is for registered PIPs that are used for comparison purposes 

only and not intended for breeding.

Acres

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Study Number: PHI-2008-139

Page 221 of 500
Table 7. Estimated Acreage per State/Territory for Nursery / Breeding Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

Bt11

MIR604

MIR162

Bt11xMIR604

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xBt11xMIR162xMIR604

1507x59122xBt11xMIR162

1507x59122xBt11xMIR604

1507xBt11xMIR162xMIR604

59122xBt11xMIR162xMIR604

1507xBt11xMIR162

AR 1 1 1 3 1111111111 10 11111111111

CO 1 1 1 3 1111111111 10 11111111111

GA 5 5 10 20 1111111111 10 11111111111

HI 10 10 20 40 20 8 6 10 2 3 1 2 1 1 54 11111111111

IA 2 2 4 8 2221111111 13 11111111111

IL 2 2 4 8 2221111111 13 11111111111

IN 1 1 1 3 1111111111 10 11111111111

KS 1 1 1 3 1111111111 10 11111111111

MI 2 2 4 8 2221111111 13 11111111111

MN 2 2 4 8 2221111111 13 11111111111

MO 2 2 4 8 2221111111 13 11111111111

MS 1 1 1 3 1111111111 10 11111111111

NE 2 2 4 8 2221111111 13 11111111111

PA 1 1 1 3 1111111111 10 11111111111

PR 10 10 30 50 30 8 6 10 2 3 1 2 1 1 64 11111111111SD 1 1 1 3 1111111111 10 11111111111

TN 1 1 1 3 1111111111 10 11111111111

Total 176 266

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Enviro(e.g. weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocation oprotocols and traits.

Category 1 Category 2 Category 3

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 7. Estimated Acreage per State/Territory for Nursery / Breeding Protocol

*

(cont'd)

State

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xBt11

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 1111111111111111 31 0 44 5 5 10 54

CO 1111111111111111 31 0 44 2 2 4 48

GA 1111111111111111 31 0 61 5 5 10 71

HI 1111111111111111 31 0 125 5 5 10 135

IA 1111111111111111 31 0 52 5 5 10 62

IL 1111111111111111 31 0 52 5 5 10 62

IN 1111111111111111 31 0 44 2 2 4 48

KS 1111111111111111 31 0 44 2 2 4 48

MI 1111111111111111 31 0 52 5 5 10 62

MN 1111111111111111 31 0 52 5 5 10 62

MO 1111111111111111 31 0 52 5 5 10 62

MS 1111111111111111 31 0 44 2 2 4 48

NE 1111111111111111 31 0 52 5 5 10 62

PA 1111111111111111 31 0 44 2 2 4 48

PR 1111111111111111 31 0 145 5 5 10 155SD 1111111111111111 31 0 44 2 2 4 48

TN 1111111111111111 31 0 44 2 2 4 48

Total 465 0 907 114 1021

** Category 4 is non-experimental material that will be used for check plots only (not breeding). * The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g. weather events, pest infestation levels), unforeseen regulatory data

Category 3 (cont'd) Category 5

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 8. Estimated Acreage per State/Territory for Yield / Agronomic Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

Bt11

MIR604

MIR162

Bt11xMIR604

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xBt11xMIR162xMIR604

1507x59122xBt11xMIR162

1507x59122xBt11xMIR604

1507xBt11xMIR162xMIR604

AR 23 22 5 50 69 12 12 69 3 3 3 3 3 3 180 484445555

CA 34 33 8 75 102 24 12 102 3 3 3 3 3 3 258 484445555

CO 115 115 25 255 345 12 12 345 3 3 3 3 3 3 732 484445555

GA 15 15 3 33 45 12 12 45 3 3 3 3 3 3 132 484445555

IA 204 204 46 454 612 60 24 612 3 3 3 3 3 3 1326 484445555

IL 117 117 26 260 351 36 12 351 3 3 3 3 3 3 768 484445555

IN 119 119 27 265 357 24 12 357 3 3 3 3 3 3 768 484445555

KS 115 115 26 256 345 12 12 345 3 3 3 3 3 3 732 484445555

MI 32 32 7 71 96 12 12 96 3 3 3 3 3 3 234 484445555

MN 160 160 36 356 480 12 12 480 3 3 3 3 3 3 1002 484445555

MO 48 48 11 107 144 12 12 144 3 3 3 3 3 3 330 484445555

MS 14 14 4 32 42 12 12 42 3 3 3 3 3 3 126 484445555

NE 88 88 20 196 264 24 12 264 3 3 3 3 3 3 582 484445555

NC 19 19 4 42 57 12 12 57 3 3 3 3 3 3 156 484445555

PA 64 64 14 142 192 12 12 192 3 3 3 3 3 3 426 484445555

SD 48 48 11 107 144 12 12 144 3 3 3 3 3 3 330 484445555

TN 48 48 11 107 144 12 12 144 3 3 3 3 3 3 330 484445555

TX 45 45 10 100 135 12 12 135 3 3 3 3 3 3 312 484445555

WI 113 112 25 250 339 12 12 339 3 3 3 3 3 3 720 484445555

Total 3158 9444

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Envweather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocation of atraits.

Category 1 Category 2 Category 3

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 8. Estimated Acreage per State/Territory for Yield / Agronomic Protocol* (cont'd)

State

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xBt11

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 8484484444848484 161 3 394 2 8 10 404

CA 8484484444848484 161 14 508 8 28 36 544

CO 8484484444848484 161 5 1153 3 11 14 1167

GA 8484484444848484 161 5 331 3 12 15 346

IA 8484484444848484 161 30 1971 17 63 80 2051

IL 8484484444848484 161 15 1204 8 29 37 1241

IN 8484484444848484 161 13 1207 7 24 31 1238

KS 8484484444848484 161 3 1152 2 9 11 1163

MI 8484484444848484 161 3 469 2 9 11 480

MN 8484484444848484 161 5 1524 3 12 15 1539

MO 8484484444848484 161 5 603 3 12 15 618

MS 8484484444848484 161 2 321 1 4 5 326

NE 8484484444848484 161 14 953 8 28 36 989

NC 8484484444848484 161 4 363 3 13 16 379

PA 8484484444848484 161 4 733 2 10 12 745

SD 8484484444848484 161 4 602 2 10 12 614

TN 8484484444848484 161 4 602 2 10 12 614

TX 8484484444848484 161 4 577 3 13 16 593

WI 8484484444848484 161 6 1137 3 13 16 1153

Total 3059 143 15804 400 16204

** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

Category 3 (cont'd) Category 5

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection 

purposes. Environmental conditions ( e.g . weather events, pest infestation levels), unforeseen regulatory data

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 9. Estimated Acreage per State/Territory for Efficacy / IRM Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

Bt11

MIR604

MIR162

Bt11xMIR604

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xBt11xMIR162xMIR604

1507x59122xBt11xMIR162

1507x59122xBt11xMIR604

1507xBt11xMIR162xMIR604

59122xBt11xMIR162xMIR604

1507xBt11xMIR162

AR 111 3 1111111111 10 11111111111

CO 111 3 1111111111 10 11111111111

HI 111 3 1111111111 10 11111111111

IA 111 3 1111111111 10 11111111111

IL 111 3 1111111111 10 11111111111

IN 111 3 1111111111 10 11111111111

MI 111 3 1111111111 10 11111111111

MN 111 3 1111111111 10 11111111111

NE 111 3 1111111111 10 11111111111

TN 111 3 1111111111 10 11111111111

TX 111 3 1111111111 10 11111111111

Total 33 110

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Env(e.g. weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocatio

protocols and traits.

Category 1 Category 2 Category 3

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 9. Estimated Acreage per State/Territory for Efficacy / IRM Protocol* (cont'd)

State

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xBt11

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 1111111111111111 31 1 45 1 3 4 49

CO 1111111111111111 31 1 45 1 3 4 49

HI 1111111111111111 31 1 45 1 3 4 49

IA 1111111111111111 31 1 45 1 3 4 49

IL 1111111111111111 31 1 45 1 3 4 49

IN 1111111111111111 31 1 45 1 3 4 49

MI 1111111111111111 31 1 45 1 3 4 49

MN 1111111111111111 31 1 45 1 3 4 49

NE 1111111111111111 31 1 45 1 3 4 49

TN 1111111111111111 31 1 45 1 3 4 49

TX 1111111111111111 31 1 45 1 3 4 49

Total 341 11 495 44 539

** Category 4 is non-experimental material that will be used for check plots only (not breeding). * The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g. weather events, pest infestation levels), unforeseen regulatory data

Category 3 (cont'd) Category 5

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 10. Estimated Acreage per State/Territory for Seed Production Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

Bt11

MIR604

MIR162

Bt11xMIR604

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

Category 3

Category 4**

Total PIP

Non-PIP

Border

Total Non-PIP

HI 40 40 100 180 100 60 60 50 5 5 5 5 5 5 300 0 0 480 100 0 10

IA 40 40 500 580 1500 60 60 50 555555 1700 0 0 2280 100 0 10

IL 10 10 950 970 1600 60 60 50 555555 1800 0 0 2770 100 0 10

NE 40 40 500 580 1500 60 60 50 555555 1700 0 0 2280 100 0 10

MI 40 40 500 580 1500 60 60 50 555555 1700 0 0 2280 100 0 10

OR 40 40 650 730 1600 60 60 50 555555 1800 0 0 2530 100 0 10

TX 40 40 100 180 100 60 60 50 5 5 5 5 5 5 300 0 0 480 100 0 10

WA 20 20 650 690 1600 60 60 50 555555 1800 0 0 2490 100 0 10

Total 4490 11100 0 0 15590 80

** Category 4 is non-experimental material that will be used for check plots only (not breeding). 

Category 5

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g. weather events, pest infestation levels) unforeseen regulatory data demands received from other countries, and other factors, necessitate a reallocation of acres between protocols and traits. 

Category 1 Category 2

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 11. Estimated Acreage per State/Territory for Regulatory Protocol*

State

1507x59122xMON810

1507xMON810

59122xMON810

Category 1 Total

1507

59122

MON810

1507x59122

Bt11

MIR604

MIR162

Bt11xMIR604

Bt11xMIR162

Bt11xMIR162xMIR604

Category 2 Total

1507x59122xMON810xMIR162

1507x59122xMON810xMIR604

1507x59122xMIR162xMIR604

1507xMON810xMIR162xMIR604

59122xMON810xMIR162xMIR604

1507x59122xBt11xMIR162xMIR604

1507x59122xBt11xMIR162

1507x59122xBt11xMIR604

1507xBt11xMIR162xMIR604

59122xBt11xMIR162xMIR604

1507xBt11xMIR162

AR 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

IA 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

IL 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

IN 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

KS 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

LA 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

MN 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

MO 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

NC 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

NE 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

OK 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

SD 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

TX 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

WI 221 5 1111111111 10 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 

Total 70 140

* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Env(e.g. weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocatio

protocols and traits.

Category 1 Category 2 Category 3

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 11. Estimated Acreage per State/Territory for Regulatory Protocol* (cont'd)

State

1507x59122xMIR604

1507xMON810xMIR162

1507xMON810xMIR604

1507xMIR162xMIR604

59122xMON810xMIR162

59122xMON810xMIR604

59122xMIR162xMIR604

MON810xMIR162xMIR604

1507xBt11

1507xMIR162

1507xMIR604

59122xMIR162

59122xMIR604

MON810xMIR162

MON810xMIR604

MIR162xMIR604

Category 3 Total

Category 4**

Total PIP

Non-PIP

Border

Total NON-PIP

Total

AR 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

IA 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

IL 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

IN 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

KS 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

LA 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

MN 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

MO 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

NC 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

NE 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

OK 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

SD 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

TX 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

WI 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 15.5 0 30.5 1 2 3 33.5

Total 217.0 0 427.0 42 469.0

** Category 4 is non-experimental material that will be used for check plots only (not breeding). * The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g. weather events, pest infestation levels), unforeseen regulatory data

Category 3 (cont'd) Category 5

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139
Table 12. Summary of EUP Program Quantities for 2010

State Category 1 Category 2 Category 3

Cry1F 

(gm)

Cry34/35Ab1 

(gm)

Cry1Ab 

(MON810)

(gm)

VIP3

 (gm)

mCry3a 

(gm)

Cry1Ab 

(Bt11)

(gm)

AR 513.47 1767.68 2007.58 10.45 134.49 1.15 117.03 3.78 4.04

CA 631.31 2171.72 1355.22 10.17 130.80 1.12 109.34 3.53 2.73

CO 2196.97 6329.97 1877.10 25.63 329.12 2.79 254.42 8.21 3.81

GA 446.13 1195.29 1616.16 7.95 102.32 0.88 87.15 2.81 3.25

HI 1877.10 3063.97 521.89 13.67 174.98 1.46 111.16 3.59 1.07

IA 8838.38 25749.16 2007.58 90.48 1160.96 9.79 860.46 27.76 4.20

IL 10488.22 21893.94 2007.58 85.68 1097.55 9.18 740.95 23.90 4.19

IN 2323.23 6717.17 2007.58 27.21 349.46 2.96 270.47 8.72 4.07

KS 2222.22 6329.97 1746.63 25.38 325.93 2.76 250.37 8.08 3.54

LA 42.09 84.18 130.47 0.63 8.09 0.07 6.65 0.21 0.26

MI 5572.39 16473.06 1877.10 59.09 758.32 6.40 568.86 18.35 3.87

MN 3131.31 8712.12 2007.58 34.18 438.78 3.71 332.31 10.72 4.08

MO 1010.10 2971.38 1746.63 14.05 180.60 1.54 146.26 4.72 3.52

MS 294.61 1144.78 1616.16 7.42 95.61 0.82 85.59 2.76 3.25

NC 395.62 1397.31 1485.69 7.99 102.82 0.88 89.37 2.88 2.99

NE 6666.67 19486.53 2007.58 69.59 892.98 7.54 666.32 21.49 4.15

OK 42.09 84.18 130.47 0.63 8.09 0.07 6.65 0.21 0.26

OR 6144.78 15151.52 0.00 52.95 678.62 5.69 469.70 15.15 0.11

PA 1220.54 3670.03 1616.16 15.98 205.37 1.74 163.87 5.29 3.26

PR 420.88 538.72 260.94 3.06 39.10 0.33 24.79 0.80 0.53

SD 968.01 2946.13 1746.63 13.88 178.39 1.52 145.48 4.69 3.52

TN 951.18 2946.13 1877.10 14.14 181.86 1.55 149.52 4.82 3.78

TX 2424.24 5319.87 1746.63 23.51 301.49 2.54 219.06 7.07 3.54

WA 5808.08 15151.52 0.00 52.05 667.17 5.60 469.70 15.15 0.10

WI 2146.46 6144.78 1485.69 24.11 309.52 2.62 236.54 7.63 3.02

Total 66776.09 177441.08 34882.15 689.87 8852.41 74.69 6582.02 212.32 71.14

Assumptions: 

 3) The g/kg of seed used for 1507 (0.0024 g/kg Cry1F), 59122 (0.031g/kg Cry34Ab1) and MON810 (0.00027 g/kg Cry1Ab) were based on the values observed

 in PHI-2008-006 (MRID 475109-04). The amount of a.i for MIR162 (0.031 g/kg VIP3A), MIR604 (0.001 g/kg mCry3A) and Bt11 (0.002 g/kg Cry1Ab) were based 

on the levels presented in Syngenta EUP application for MIR162.

Kgs of seed per Category

 1) Assumed 100% of the EUP test plants (Category 1) were 1507x59122xMON810 (0.0027g/kg Cry1F, 0.034 g/kg Cry34/35Ab1 and 0.00026g/kg 

Cry1Ab). Values based on expression levels observed in PHI-2008-006 (MRID 475109-04).

 2) Assumed 100% of the acres in Category 2 and Category 3 were planted to each registered single event (i.e. 1507, 59122, MON810, MIR162 and MIR604)

except for Bt11 (Category 2 only). 

Total A.I. Quantities

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 231 of 500
 

G3. Details of the Proposed EUP Program 

Crop: Corn (Zea mays L.)

Target Pests: 

Agrotis ipsilon (Black cutworm) 

Helicoverpa zea (Corn earworm or cotton bollworm) 

Ostrinia nubilalis (European corn borer) 

Spodoptera frugiperda (Fall armyworm) 

Diatraea grandiosella (Southwestern corn borer) 

Richia albicosta (Western bean cutworm) 

Elasmopalpus lignosellus (Lesser corn stalk borer) 

Diatrae crambidoides (Southern corn stalk borer) 

Diatrea saccharalis (Sugarcane borer) 

Papaipema nebris (Common stalk borer) 

Diabrotica virgifera virgifera (western corn rootworm) 

Diabrotica barberi (northern corn rootworm) 

Diagrotica virgifera zeae (Mexican corn rootworm) 

Major Geographic Areas: 25 states and Puerto Rico, representing diverse agronomic zones

Sites: Pioneer research centers, seed production fields, cooperator fields, privately owned farms and 

university farms and testing sites. All EUP Blocks will be planted on existing agricultural land.

Period of Use: February 1, 2010 through June 30, 2011.

Protocols for Field Experimentation: 

Research planned under the EUP Program will be conducted under the following five protocols: 

1. Nursery / Breeding; 

2. Yield / Agronomic; 

3. Efficacy / IRM; 

4. Inbred and Hybrid Seed Production; and 

5. Regulatory. 

G4. Definitions 

EUP Test Plants 

Plants that contain 1507x59122xMON810, 1507xMON810 or 59122xMON810. 

EUP Experiment 

An Experiment is the unit of measure used to coordinate and track specific field activities. 

Experiment ID’s are assigned to each Experiment. Experiments containing EUP Test Plants are 

defined as EUP Experiments. EUP Experiments may also contain registered PIPs for breeding or 

comparison (if listed on the Master Label), non-registered PIPs (if listed on the Master Label), 

registered PIPs for comparison only (i.e., “commercial checks”), and/or non-PIPs (such as 

herbicide-tolerant or non-transgenic corn). 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 232 of 500
 

EUP Block 

An area of land in which EUP Experiments are planted (including filler corn, if planted between EUP 

Experiments) and may consist of one, several or many EUP Experiments. An EUP block may be 

planted directly adjacent to non-EUP experiments or blocks. An EUP block is defined by the adjacent 

nature of the EUP experiments it contains. . 

G5. Universal Protocol Procedures 

The following procedures will be used for all EUP Experiments. 

Shipping and Labeling 

 Each shipment containing EUP seed must contain a copy of the EUP Master Label. A Master 

Label Weight Summary may be used to summarize the amount of seed in the shipment, so long 

as it accompanies the EUP Master Label. See Figure 3 for an example of a Master Label Weight 

Summary. 

 Packaging from seed shipments is typically destroyed after shipments have been received but 

may be re-used after cleaning. 

Schedule 

For the continental U.S., planting of trials generally takes place from mid February through the month 

of June. Observations and harvesting generally occur after planting through August to November. For 

Hawaii and Puerto Rico, there may be multiple plantings and harvest cycles throughout the year. 

Planting 

 Seed may be planted by hand or machine. 

o Dispose of any remaining seed according to accepted disposition methods. 

o Do not re-use seed packets. 

 No special cleaning of planting equipment is required before or after planting any seed planted 

under this EUP. 

 Border rows may be used for agronomic purposes, but are not required under this EUP Program. 

Marking EUP Experiments 

The EUP block will be identified with stakes or other marking options. If equipment available, GPS 

coordinates may be used to identify the EUP block. 

Gene Containment 

No containment or isolation procedures are required because the events planted under this EUP are 

all individually registered by EPA. In addition, all proteins have full tolerance exemptions.

Harvest Procedures 

 Vegetative material or grain will be harvested by hand or machine.

 Any retained seed will be clearly labeled and stored in a secured facility.

 No special cleaning of harvest equipment is required before or after harvesting material planted 

under this EUP.

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 233 of 500
 

Final Disposition of Harvested Grain or Vegetative Material 

Grain and vegetative material harvested under this EUP may enter food/feed streams through normal 

commercial channels. 

Post-Trial Monitoring 

None required. 

Storing EUP Material 

 EUP Test Plant seed will be stored in a secure facility with a sign stating that regulated, 

genetically modified material is stored inside. 

 EUP Test Plant seed will be labeled to enable ready identification of materials. Examples of 

labeling include (but are not limited to) color coding, marking, tagging, or otherwise distinguishing 

seed to allow immediate recognition. 

Termination of the EUP 

The EUP Test Plants are expected to receive commercial registration in the early spring of 2010. This 

EUP extension is being submitted as a contingency for the unlikely scenario that the registrations are 

delayed and plantings need to occur in the early spring. 

In the event this EUP contingency plan is needed and plantings under the EUP occur, there may be 

EUP Experiments that do not contain any Category 2 or Category 3 events/combinations. For those 

experiments, record keeping will continue until such time as the EUP Test Plants are fully registered at 

the federal and state level. At that time, the EUP provisions will terminate. 

For experiments planted under this EUP that contain Category 2 or Category 3 events combinations, 

record keeping will continue through the termination of the experiment. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 234 of 500
 

 Figure 1. Example of Master Label Weight Summary 

Master Label Weight Summary 

29964-EUP-6 

Event Weight (lbs) 

1507xMON810 0.1 

1507x59122xMON810 0.1 

1507 0.05 

MON810 0.05 

Bt11xMIR162 0.03 

Bt11 0.03 

MIR162 0.02 

59122 0.02 

MIR604 0.01 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 235 of 500
 

Nursery / Breeding Protocol 

General Objectives 

 Introgress events 1507, 59122 and MON810 into elite inbred corn lines. 

 Produce research seed for evaluation in field, greenhouse and/or laboratory trials.

 Collect phenotypic data on converted inbred lines. 

 Collect plant tissue on converted inbred lines for laboratory analysis. 

Description 

Inbreds may be hand pollinated (selfed, backcrossed or crossed to tester lines) to produce new inbred 

and hybrids for evaluation. Some inbreds may be open pollinated in isolation to produce seed for 

phenotypic analysis. Plants may be treated with herbicides and/or insecticides. Seed is generally 

retained for future breeding or testing purposes. 

Gene Containment 

No containment or isolation procedures are required. 

Harvest Procedures 

 Hand or machine harvest. 

 Store retained seed in a clearly labeled and secure facility. 

 No special cleaning of harvest equipment is required before or after harvesting material planted 

under this EUP. . 

Final Disposition of EUP Material 

Grain and vegetative material harvested under this EUP may enter food/feed streams through 

normal commercial channels. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 236 of 500
 

Yield / Agronomic Protocol 

General Objectives 

 Assess the agronomic impact of combining the 1507, 59122 and MON810 events in various 

hybrid backgrounds under various environmental and insect pressure conditions. 

 Assess the yield and agronomic properties of EUP test inbreds and hybrids compared to other 

single and stacked event inbreds and hybrids (both registered and non-registered PIP single 

events and breeding stacks), commercial check hybrids (registered PIPs), conventional 

hybrids, and/or insecticide treatments. 

 Assess the herbicide tolerance of EUP Test Plants (including EUP Test Plants that have been 

crossed with non-PIP herbicide tolerant lines of corn). 

Description 

EUP Blocks will generally be planted in multiple locations using randomized block design, strips or 

single large blocks depending on study type. Seed may be treated with a variety of insecticidal seed 

treatments or blended with non-transgenic or transgenic corn to evaluate agronomic performance 

including yield. Plants may be treated with herbicide and/or insecticides. Commercial checks may be 

planted as comparators. Harvested grain is generally not retained for breeding purposes. 

Gene Containment 

No containment or isolation procedures are required. 

Harvest Procedures 

 Hand or machine harvest. 

 Store retained seed in a clearly labeled and secure facility. 

 No special cleaning of harvest equipment is required before or after harvesting material planted 

under this EUP. 

Final Disposition of EUP Material 

Grain and vegetative material harvested under this EUP may enter food/feed streams through 

normal commercial channels. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 237 of 500
 

Efficacy / IRM Protocol 

General Objectives 

 Assess the efficacy of the EUP Test Plants against key target and secondary pests in corn. 

 Generate data for use in the refinement of IRM strategies for corn containing events 1507, 

MON810 and/or 59122. 

 Characterize the refuge deployment methods for the EUP Test Plants. 

Description 

Test plots will generally be planted in multiple locations using randomized block design, strips or single 

large blocks depending on study type. Seed may be treated with a variety of insecticidal seed 

treatments or blended with non-transgenic or transgenic corn to evaluate various methods of refuge 

deployment. Artificial or natural infestations of lepidopteran and coleopteran may be used in these 

trials. Plants may be treated with herbicide and/or insecticides. Harvested grain is generally not 

retained for breeding purposes. 

Gene Containment 

No containment or isolation procedures are required. 

Harvest Procedures 

 Hand or machine harvest. 

 Clean harvest equipment (i.e., combine) while in the regulated transgenic site. 

 Store retained seed in a clearly labeled and secure facility. 

 No special cleaning of harvest equipment is required before or after harvesting material planted 

under this EUP. 

Final Disposition of EUP Material 

Grain and vegetative material harvested under this EUP may enter food/feed streams through 

normal commercial channels. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 238 of 500
 

Inbred and Hybrid Seed Production Protocol 

General Objectives 

 Increase inbred and hybrid seed for furthertesting and product development in various genetic 

backgrounds 

Description 

EUP Blocks (approximately 0.1 to 15 acres in size) of individual inbred lines may be increased by 

open pollination. Hybrid seed may be produced (in EUP Blocks approximately 1 to 30 acres in size) 

by planting two inbred lines and allowing open pollination (by using a male sterile inbred or removing 

the tassels from one of the inbreds). Plants may be treated with herbicides and/or insecticides. 

Gene Containment 

No containment or isolation procedures are required. 

Harvest Procedures 

 Hand or machine harvest. 

 

 Store retained seed in a clearly labeled and secure facility. 

 No special cleaning of harvest equipment is required before or after harvesting material planted 

under this EUP. 

Final Disposition of EUP Material 

 Grain and vegetative material harvested under this EUP may enter food/feed streams through 

normal commercial channels.. 

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Study Number: PHI-2008-139

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Regulatory Protocol 

General Objectives: 

 Conduct field trials as needed to support global regulatory submissions

 Generate plant tissue samples from EUP Test Plants (inbreds and hybrids) for use in 

regulatory characterization studies.

 Collect phenotypic data for use in global regulatory submissions

 Produce grain for use in animal feeding studies

Description 

Test plots will generally be planted in multiple locations using randomized block design, strips or single 

large blocks depending on study type. Agronomic characteristics and plant tissue samples may be 

collected at various developmental growth stages. Plants may be treated with herbicide and/or 

insecticides. 

Gene Containment 

No containment or isolation procedures are required: 

Harvest Procedures 

 Hand or machine harvest. 

 Store retained seed in a clearly labeled and secure facility. 

 No special cleaning of harvest equipment is required before or after harvesting material planted 

under this EUP 

Final Disposition of EUP Material 

Grain and vegetative material harvested under this EUP may enter food/feed streams through 

normal commercial channels. 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 240 of 500
CONFIDENTIAL ATTACHMENT 

{CBI Cross Reference Number 1} 

EPA Form 8570-4

Confidential Statement of Formula 

The Certified Limits presented in the attached CSFs represent the highest and lowest levels of 

individual proteins measured during the course of studies on events or event combinations for each 

plant tissue type. When data measurements are not available for event combinations, expression of 

proteins is assumed to be additive (based on individual events or combinations with known protein 

levels). No entries are being made for the Nominal Concentration (column 13) of each protein 

because their levels vary significantly by tissue type and by growth stages of the plants. There is not 

an expected amount of each ingredient in a typical plant sample. 

Pioneer is proposing a set of non-standard Certified Limits as allowed under 40 CFR § 158.175(c). 

These proteins are plant-incorporated protectants or marker proteins produced by the plant, not in a 

traditional closely monitored and controlled manufacturing facility. Multiple factors beyond the control 

of the Applicant could influence the levels of these proteins synthesized by individual plants, including 

but not limited to environmental conditions, plant growth stage, and the presence of biotic or abiotic 

stressors. For these reasons, the Applicant is not providing the Certification required by 40 CFR § 

158.175(e). 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 241 of 500
{CBI Cross Reference Number 2} 

Pioneer Hi-Bred International, Inc. 

Study Number: PHI-2008-139

Page 497 of 500