# Patent

## Title
Coatings containing polymer modified enzyme for stable self-cleaning of organic stains

## Abstract

Temporary active coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

## Background
FIELD OF THE INVENTION
The present invention relates generally to coating compositions including active substances and methods of their use to facilitate removal of organic stains. In specific embodiments, the invention relates to compositions and methods for prevention of insect stain adherence to a surface as well as insect stain removal by incorporating a chemically modified protein into base materials to degrade insect body components.
BACKGROUND OF THE INVENTION
Many outdoor surfaces are subject to stain or insult from natural sources such as bird droppings, resins, and insect bodies. As a result, the resulting stain often leaves unpleasant marks on the surface deteriorating the appearance of the products.
Traditional self-cleaning coatings and surfaces are typically based on water rolling or sheeting to carry away inorganic materials. These show some level of effectiveness for removal of inorganic dirt, but are less effective for cleaning stains from biological sources, which consist of various types of organic polymers, fats, oils, and proteins each of which can deeply diffuse into the subsurface of coatings. Prior art approaches aim to reduce the deposition of stains on a surface and facilitate its removal by capitalizing on the “lotus-effect” where hydrophobic, oleophobic and super-amphiphobic properties are conferred to the surface by polymeric coatings containing appropriate nanocomposites. An exemplary coating contains fluorine and silicon nanocomposites with good roll off properties and very high water and oil contact angles. When used on rough surfaces like sandblasted glass, nanocoatings may act as a filler to provide stain resistance. A drawback of these “passive” technologies is that they are not optimal for use in high gloss surfaces because the lotus-effect is based on surface roughness.
Photocatalytic coatings are promising for promoting self-cleaning of organic stains. Upon the irradiation of sun light, a photocatalyst such as Ti02 chemically breaks down organic dirt that is then washed away by the water sheet formed on the super hydrophilic surface. As an example, the photocatalyst Ti02 was used to promote active fingerprint decomposition of fingerprint stains in U.S. Pat. Appl. Publ. 2009/104086. A major drawback to this technology is its limitation to use on inorganic surfaces due to the oxidative impairment of the polymer coating by Ti02. Also, this technology is less than optimal for automotive coatings due to a compatibility issue: Ti02 not only decomposes dirt, but also oxidizes polymer resins in paint.
Therefore, there is a need for new materials or coatings that can actively promote the removal of organic stains on surfaces or in coatings and minimize the requirement for maintenance cleaning.
SUMMARY OF THE INVENTION
A process of facilitating the removal of organic stains is provided including providing a water-stabilized active temporary coating material formed by associating a chemically modified enzyme with a base and coating a substrate with the active coating material such that the enzyme is capable of enzymatically degrading a component of an organic stain in contact with the active coating material.
A water stabilized active temporary coating material is optionally capable of degrading a component of an organic stain following immersion of said coating in water for 30 minutes or more, optionally where the coating retains 50% or more activity following immersion in water for 30 minutes.
A chemically modified enzyme is optionally a hydrolase such as a bacterial neutral thermolysin-like-protease, an amylase, or a lipase. The enzyme is chemically modified by a polymeric moiety, optionally by at least one molecule of polyoxyethylene. The polyoxyethylene optionally has a molecular weight between 1,000 and 15,000 Daltons. In some embodiments, the polyoxyethylene further includes a succinimidyl ester prior to reaction with said enzyme. A polymeric moiety is optionally directly or indirectly covalently bound to an amino group on the enzyme such as a terminal amino group or on a lysine. In some embodiments a polymeric moiety is directly or indirectly covalently bound to a cysteine within the enzyme. It is appreciated that a polymeric moiety is optionally linear or branched.
A water-stabilized active temporary coating material optionally is covalently attached to at least one component of the base or is non-covalently adhered to or admixed into the base. Such coatings when present on a substrate optionally have a surface activity of 0.0075 Units/cm2 or greater when the coating includes a thermolysin as an enzyme.
The water-stabilized active temporary coating materials optionally include a first polyoxyethylene associated with the base that is independent of the enzyme. It is appreciated that the ratio of the base to the enzyme in a coating is optionally 2:1 to 20:1 by weight respectively. A composition optionally includes an enzyme that is chemically modified with a second polyoxyethylene. A first or second polyoxyethylene optionally has a molecular weight between 1,000 and 15,000 Daltons. A first and second polyoxyethylene optionally have equal monomers of oxyethylene. A first polyoxyethylene is optionally derivatized such as with a succininimidyl ester. A second polyoxyethylene is optionally derivatized such as with a succininimidyl ester prior to reaction with an enzyme.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a schematic for forming a water-stabilized active temporary coating composition according to one embodiment of the invention;
FIG. 2 is a schematic of chemical modification of an enzyme and its incorporation into a base according to one embodiment of the invention;
FIG. 3 illustrates homogenous incorporation of a chemically modified enzyme into a base according to one embodiment of the invention;
FIG. 4 illustrates water-stability of a coating incorporating a chemically modified enzyme as measured by residual activity after water washing (A) or water contact angle before and after water washing (B);
FIG. 5 demonstrates facilitated removal of food stains on a water-stabilized active temporary coating after application to a substrate.



DETAILED DESCRIPTION OF EMBODIMENTS OF THE INVENTION
The following description of embodiment(s) of the invention is merely exemplary in nature and is in no way intended to limit the scope of the invention, its application, or uses, which may, of course, vary. The invention is described with relation to the non-limiting definitions and terminology included herein. These definitions and terminology are not designed to function as a limitation on the scope or practice of the invention but are presented for illustrative and descriptive purposes only.
A composition useful as a coating is provided where enzymes associated with the coating material are modified so as to improve enzyme activity lifetime during and following exposure of a coating to water. The coatings provided herein are temporary coatings that have several advantages over other coating materials that are used as a permanent coating and are not intended to be renewed over the useful lifetime of a coated article. Temporary coatings are relatively simple to apply and can be done by a layman in a home situation or by professionals. Use of temporary coatings containing modified enzymes of the present invention allows one to regularly renew the bioactive surface as well as improve other qualities such as shine, protection from the elements, and water runoff.
The coatings of the present invention demonstrate resistance to loss of enzyme activity due to weathering. Weathering as defined herein includes exposure to water, heat, UV light, or other insult either in the environment or in a laboratory. Coatings according to the present invention have unexpected resistance to weathering by exposure to water, such as water immersion. As such, the term weathering includes immersion in water.
It is appreciated that while the description herein is directed to coatings, the materials described herein may also be substrates or articles that do not require a coating thereon for promotion of organic stain removal. As such, the word “coating” as used herein means a material that is operable for layering on a surface of one or more substrates, or may comprise the substrate material itself. In some embodiments, a “coating” is exclusive of a substrate such that it is a material that may be used to overlay a substrate. As such, the methods and compositions disclosed herein are generally referred to as an enzyme associated with a coating for exemplary purposes only. One of ordinary skill in the art appreciates that the description is equally applicable to substrates themselves.
The present invention is based on the catalytic activity of an enzyme to selectively degrade components of organic stains, thus, promoting active stain removal. Organic stains illustratively include organic polymers, fats, oils, or proteins. Inventive compositions and processes are provided for the active breakdown of organic stains by a water-stabilized active temporary coating. Temporary coating materials of the prior art have the capability to degrade organic stains, but the inventors unexpectedly discovered that, unlike permanent coatings, these temporary coatings are rapidly inactivated upon exposure to water such that the expected life of the coating is reduced to the point of uselessness. Among the nearly infinite possible mechanisms of promoting enzyme stability, the inventors discovered that the addition of one or more polymeric moieties on an enzyme prior to incorporation with a base provides for dramatically improved water-stability of the resulting coating material.
As such, a water-stabilized active temporary coating material composition is provided including a base with an associated chemically modified enzyme, and optionally a first polyoxyethylene also associated with the base, where the first polyoxyethylene is independent of the enzyme (i.e. not covalently linked to the enzyme). A composition has utility as a coating for the self-cleaning of organic stains such as food stains, insect stains, fingerprints, and other environmental or artificial insults.
A composition is a water-stabilized coating. The term “water-stabilized” denotes activity of the coating toward the self-cleaning or loosening of an associated organic stain, where the activity is increased by the presence of a chemically modified protein relative to the identical coating with a non-chemically modified protein. Water-stabilized optionally includes coatings that retain 50% to 90%, or any value or range therebetween, or more activity after coating immersion in water for 30 minutes. Water-stabilized optionally includes coatings that retain 15% or greater activity after coating immersion in water for 90 minutes.
A composition is a temporary coating. As used herein the term “temporary” is defined as operable for a time between 30 minutes and three months. It is appreciated that the outer limit of temporary is optionally defined by the environmental conditions a coating is subjected to. Optionally, temporary is any time between application of an inventive composition and subsequent immersion in or contact with water. In some embodiments, temporary is at or less than three months, optionally, less than 2 months, optionally less than 6, 5, 4, 3, 2, or 1 weeks, or any time or range of time therebetween. Optionally, temporary is at or less than 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, or 1 day, or any time or range therebetween. In some embodiments, the term “temporary” is any time between application of an inventive composition to a substrate and immersion or contact with water for 30, 60, or 90 minutes, or more.
A composition includes a base material. As used herein a base is any commercially or otherwise available automotive, furniture, floor, shoe, metal, or other surface conditioner, polish or protectant known in the art. Illustrative examples of a base include: naturally derived waxes illustratively paraffin wax, microcrystalline petroleum wax, carnauba wax, candelilla vegetable wax, montan coal derived wax; synthetic polymeric waxes such as oxidized polyethylene; silicone-based waxes illustratively those found in U.S. Pat. No. 7,753,998, dimethylsilicones, aminofunctional silicones; a nonionic or anionic surfactant in water composition illustratively that described in U.S. Pat. Nos. 5,073,407 and 5,968,238; and other materials commonly used for surface conditioning, polish, or protection; and combinations thereof.
Specific examples of bases illustratively include polishes intended for use on automobiles. Automobile polishes illustratively include: 1) TURTLE WAX carnauba car wax T-6 (carnauba wax containing silicone resin in petroleum distillates); 2) DURA SHINE as disclosed in U.S. Pat. No. 5,073,407; 3) PLASTX a synthetic polymer auto polish; 4) TURTLE WAX ICE synthetic polish which is a synthetic blend of hydrocarbons and silicon resins; 5) EAGLE ONE NANOWAX described in U.S. Pat. No. 7,503,963; 6) NU FINISH NF-76 as described in U.S. Pat. No. 7,067,573; and 7) TURTLE WAX PLATINUM series wax which is a blend of brazilian carnauba with bavarian montan wax along with light reflective polymers, are purchased from a local auto parts supplier.
A composition includes at least one active protein. An active protein is a macromolecule that has functional activity such as that of an enzyme illustratively a protease or hydrolase. A “protein” as defined herein has three or more natural, synthetic, or derivative amino acids covalently linked by a peptide bond and possessing the activity of an enzyme. Accordingly, the term “protein” as used herein include between 3 and about 1000 or more amino acids or having a molecular weight in the range of about 150-350,000 Daltons. A protein is a molecule with a contiguous molecular sequence three amino acids or greater in length, optionally matching the length of a biologically produced proteinaceous molecule encoded by the genome of an organism. Examples of proteins include an enzyme, an antibody, a receptor, a transport protein, a structural protein, or a combination thereof. Proteins are capable of specifically interacting with another substance such as a ligand, drug, substrate, antigen, or hapten. It is appreciated that a protein is chemically modified by the addition of one or more homo or heteropolymeric moieties as described herein. The term “analogue” is exclusive of chemical modification with a homo or heteropolymeric group with the exception of biotinylation.
A protein is optionally modified from a naked polypeptide sequence such as by the addition or subtraction of one or more molecules of phosphorus, sulfur, or by the addition of a pendent group such as a biotin, avidin, fluorophore, lumiphore, or other pendent group suitable for purification, detection, or altering solubility or other characteristic of a protein.
The description herein is directed to a protein that is an enzyme, but it is appreciated that other protein active components are similarly operable herein. An enzyme is optionally a bioactive enzyme. A bioactive enzyme is capable of cleaving a chemical bond in a molecule that is found in a biological organism, the environment, or in food. An enzyme is optionally a protease that is capable of cleaving a peptide bond illustratively including a bacterial protease, or analogue thereof. A protein that functions as an enzyme is optionally identical to the wild-type amino acid sequence encoded by a gene, a functional equivalent of such a sequence, or a combination thereof. A protein is referred to as “wild-type” if it has an amino acid sequence that matches the sequence of a protein as found in an organism in nature. It is appreciated that a protein is optionally a functional equivalent to a wild-type enzyme, which includes a sequence and/or a structural analogue of a wild-type protein's sequence and/or structure and functions as an enzyme. The functional equivalent enzyme may possess similar or the same enzymatic properties as a wild-type enzyme, such as catalyzing chemical reactions of the wild-type enzyme's EC classification, and/or may possess other enzymatic properties, such as catalyzing the chemical reactions of an enzyme related to the wild-type enzyme by sequence and/or structure. An enzyme encompasses its functional equivalents that catalyze the reaction catalyzed by the wild-type form of the enzyme (e.g., the reaction used for EC Classification). As an illustrative non-limiting example, the term “amylase” encompasses any functional equivalent of an amylase that retains amylase activity though the activity may be altered such as by increased reaction rates, decreased reaction rates, altered substrate preference, increased or decreased substrate binding affinity, etc. Examples of functional equivalents include mutations to a wild-type enzyme sequence, such as a sequence truncation, an amino acid substitution, an amino acid modification, and/or a fusion protein, etc., wherein the altered sequence functions as an enzyme.
An enzyme is either immobilized into or on coatings and catalyzes the degradation of organic stain components into smaller molecules. Without being limited to one particular theory, the smaller product molecules are less strongly adherent to a surface or coating such that gravity or gentle rinsing such as with water, air, or other fluid promotes removal of the organic stain material from the coating. Thus, the invention has utility as a composition and method for the active removal of organic stains from surfaces.
Enzymes are generally described according to standardized nomenclature as Enzyme Commission (EC) numbers. Examples of enzymes operable herein include: EC1, oxidoreductases; EC2, transferases; EC3, hydrolases; EC4, lyases; EC5, isomerases; or EC6, ligases. Enzymes in any of these categories can be included in a composition according to embodiments of the present invention.
In particular embodiments, an included enzyme is a hydrolase such as a glucosidase, a protease, or a lipase. Non-limiting examples of particular glucosidases include amylases, chitinase, and lysozyme. Non-limiting examples of particular proteases include trypsin, chymotrypsin, thermolysin, subtilisin, papain, elastase, and plasminogen. Non-limiting examples of lipases include pancreatic lipase and lipoprotein lipase. Illustrative examples of proteins that function as enzymes are included in U.S. Patent Application Publication No: 2010/0210745.
Amylase is an enzyme present in some embodiments of a coating composition. Amylases have activity that break down starch. Several types of amylases are operable herein illustratively including α-amylase (EC 3.2.1.1) responsible for endohydrolysis of (1→4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides. α-Amylase is illustratively derived from Bacillus subtilis and has the sequence found at Genbank Accession No: ACM91731 (SEQ ID NO: 1), or an analogue thereof and encoded by the nucleotide sequence of SEQ ID NO: 2. A specific example is α-amylase from Bacillus subtilis available from Sigma-Aldrich Co., St. Louis, Mo. Additional α-amylases include those derived from Geobacillus stearothermophilus (Accession No: AAA22227), Aspergillus oryzae (Accession No: CAA31220), Homo sapiens (Accession No: BAA14130), Bacillus amyloliquefaciens (Accession No: ADE44086), Bacillus licheniformis (Accession No: CAA01355), or other organism, or analogues thereof. It is appreciated that β-amylases, γ-amylases, or analogues thereof from a variety of organisms are similarly operable in a protein-polymer composition.
Specific examples of amylase enzymes illustratively have 1000 U/g protease activity or more wherein one (1) U (unit) is defined as the amount of enzyme that will liberate the non-protein digestion product form potato starch of Zulkowsky (e.g. starch, treated with glycerol at 190° C.; Ber. Deutsch. Chem. Ges, 1880; 13:1395). Illustratively, the amylase has activity anywhere at or between 1,000 U/g to 500,000 U/g, or greater. It is appreciated that lower activities are operable.
A protease is optionally a bacterial metalloprotease such as a member of the M4 family of bacterial thermolysin-like proteases of which thermolysin is the prototype protease (EC 3.4.24.27) or analogues thereof. A protease is optionally the bacterial neutral thermolysin-like-protease (TLP) derived from Bacillus stearothermophilus (Bacillus thermoproteolyticus Var. Rokko) (illustratively sold under the trade name “THERMOASE C160” available from Amano Enzyme U.S.A., Co. (Elgin, Ill.)) or analogues thereof. A protease is optionally any protease presented in de Kreig, et al., J Biol Chem, 2000; 275(40):31115-20. Illustrative examples of a protease include the thermolysin-like-proteases from Bacillis cereus (Accession No. P05806), Lactobacillis sp. (Accession No. Q48857), Bacillis megaterium (Accession No. Q00891), Bacillis sp. (Accession No. Q59223), Alicyclobacillis acidocaldarious (Accession No. Q43880), Bacillis caldolyticus (Accession NO. P23384), Bacillis thermoproteolyticus (Accession No. P00800), Bacillus stearothermophilus (Accession No. P43133), Bacillus subtilis (Accession No. P06142), Bacillus amyloliquefaciens (Accession No. P06832), Lysteria monocytogenes (Accession No: P34025; P23224), among others known in the art.
A wild-type protease is a protease that has an amino acid sequence identical to that found in an organism in nature. An illustrative example of a wild-type protease is that found at GenBank Accession No. P06874 and SEQ ID NO: 3, with the nucleotide sequence encoding SEQ ID NO: 3 found in Takagi, M., et al., J Bacteriol., 1985; 163(3):824-831 and SEQ ID NO: 4.
Methods of screening for protease activity are known and standard in the art. Illustratively, screening for protease activity in a protease protein or analogue thereof illustratively includes contacting a protease or analogue thereof with a natural or synthetic substrate of a protease and measuring the enzymatic cleavage of the substrate. Illustrative substrates for this purpose include casein of which is cleaved by a protease to liberate folin-positive amino acids and peptides (calculated as tyrosine) that are readily measured by techniques known in the art. The synthetic substrate furylacryloylated tripeptide 3-(2-furylacryloyl)-L-glycyl-L-leucine-L-alanine obtained from Bachem AG, Bubendorf, Switzerland is similarly operable.
Specific examples of proteases illustratively have 10,000 Units/g protease activity or more. In some embodiments, a protease is a thermolysin wherein one (1) U (unit) is defined as the amount the enzyme that will liberate the non-proteinous digestion product from milk casein (final concentration 0.5%) to give Folin's color equivalent to 1 μmol of tyrosine per minute at the reaction initial reaction stage when a reaction is performed at 37° C. and pH 7.2. Illustratively, the protease activity is anywhere between 10,000 PU/g to 1,500,000 U/g inclusive or greater. It is appreciated that lower protease activities are operable. Protease activity is optionally in excess of 300,000 U/g. Optionally, protease activity is between 300,000 U/g and 2,000,000 U/g or higher.
A protein is optionally a lipase. A wild-type lipase is a lipase that has an amino acid sequence identical to that found in an organism in nature. An illustrative example of a wild-type lipase is that found at GenBank Accession No. ACL68189 and SEQ ID NO: 5. An exemplary nucleotide sequence encoding a wild-type lipase is found at Accession No. FJ536288 and SEQ ID NO: 6.
Lipase activity is illustratively defined in Units/gram. 1 Unit illustratively corresponds to the amount of enzyme that hydrolyzes 1 μmol acetic acid per minute at pH 7.4 and 40° C. using the substrate triacetin (Sigma-Aldrich, St. Louis, Mo., Product No. 90240). The lipase of SEQ ID NO: 5 may have an activity of 200 Units/gram.
Methods of screening for lipase activity are known and standard in the art. Illustratively, screening for lipase activity in a lipase protein or analogue thereof illustratively includes contacting a lipase or analogue thereof with a natural or synthetic substrate of a lipase and measuring the enzymatic cleavage of the substrate. Illustrative substrates for this purpose include tributyrin and triacetin both of which are cleaved by a triacylglycerol lipase to liberate butyric acid or acetic acid, respectively, that is readily measured by techniques known in the art.
A protein optionally functions with one or more cofactor ions or proteins. A cofactor ion is illustratively a zinc, cobalt, or calcium.
Cloning, expressing, and purifying any protein operable herein is achievable by methods ordinarily practiced in the art illustratively by methods disclosed in: Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, ed. Sambrook et al., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989; Current Protocols in Molecular Biology, ed. Ausubel et al., Greene Publishing and Wiley-Interscience, New York, 1992 (with periodic updates); and Short Protocols in Molecular Biology, ed. Ausubel et al., 52 ed., Wiley-Interscience, New York, 2002.
Naturally derived amino acids present in a protein illustratively include the common amino acids alanine, cysteine, aspartic acid, glutamic acid, phenylalanine, glycine, histidine, isoleucine, lysine, leucine, methionine, asparagine, proline, glutamine, arginine, serine, threonine, valine, tryptophan, and tyrosine. It is appreciated that less common derivatives of amino acids that are either found in nature or chemically altered are optionally present in a protein as well such as alpha-asparagine, 2-aminobutanoic acid or 2-aminobutyric acid, 4-aminobutyric acid, 2-aminocapric acid (2-aminodecanoic acid), 6-aminocaproic acid, alpha-glutamine, 2-aminoheptanoic acid, 6-aminohexanoic acid, alpha-aminoisobutyric acid (2-aminoalanine), 3-aminoisobutyric acid, beta-alanine, allo-hydroxylysine, allo-isoleucine, 4-amino-7-methylheptanoic acid, 4-amino-5-phenylpentanoic acid, 2-aminopimelic acid, gamma-amino-beta-hydroxybenzenepentanoic acid, 2-aminosuberic acid, 2-carboxyazetidine, beta-alanine, beta-aspartic acid, biphenylalanine, 3,6-diaminohexanoic acid, butanoic acid, cyclobutyl alanine, cyclohexylalanine, cyclohexylglycine, N5-aminocarbonylornithine, cyclopentyl alanine, cyclopropyl alanine, 3-sulfoalanine, 2,4-diaminobutanoic acid, diaminopropionic acid, 2,4-diaminobutyric acid, diphenyl alanine, N,N-dimethylglycine, diaminopimelic acid, 2,3-diaminopropanoic acid, S-ethylthiocysteine, N-ethylasparagine, N-ethylglycine, 4-aza-phenylalanine, 4-fluoro-phenylalanine, gamma-glutamic acid, gamma-carboxyglutamic acid, hydroxyacetic acid, pyroglutamic acid, homoarginine, homocysteic acid, homocysteine, homohistidine, 2-hydroxyisovaleric acid, homophenylalanine, homoleucine, homoproline, homoserine, homoserine, 2-hydroxypentanoic acid, 5-hydroxylysine, 4-hydroxyproline, 2-carboxyoctahydroindole, 3-carboxyisoquinoline, isovaline, 2-hydroxypropanoic acid (lactic acid), mercaptoacetic acid, mercaptobutanoic acid, sarcosine, 4-methyl-3-hydroxyproline, mercaptopropanoic acid, norleucine, nipecotic acid, nortyrosine, norvaline, omega-amino acid, ornithine, penicillamine (3-mercaptovaline), 2-phenylglycine, 2-carboxypiperidine, sarcosine (N-methylglycine), 2-amino-3-(4-sulfophenyl)propionic acid, 1-amino-1-carboxycyclopentane, 3-thienylalanine, epsilon-N-trimethyllysine, 3-thiazolylalanine, thiazolidine 4-carboxylic acid, alpha-amino-2,4-dioxopyrimidinepropanoic acid, and 2-naphthylalanine.
A protein is obtained by any of various methods known in the art illustratively including isolation from a cell or organism, chemical synthesis, expression of a nucleic acid sequence, and partial hydrolysis of proteins. Chemical methods of protein synthesis are known in the art and include solid phase peptide synthesis and solution phase peptide synthesis or by the method of Hackeng, T M, et al., Proc Natl Acad Sci USA, 1997; 94(15):7845-50. A protein may be a naturally occurring or non-naturally occurring protein. The term “naturally occurring” refers to a protein endogenous to a cell, tissue or organism and includes allelic variations. A non-naturally occurring protein is synthetic or produced apart from its naturally associated organism or is modified and is not found in an unmodified cell, tissue or organism.
Modifications and changes can be made in the structure of a protein and still obtain a molecule having similar characteristics as an active enzyme (e.g., a conservative amino acid substitution). For example, certain amino acids can be substituted for other amino acids in a sequence without appreciable loss of activity or optionally to reduce or increase the activity of an unmodified protein. Because it is the interactive capacity and nature of a protein that defines that protein's functional activity, certain amino acid sequence substitutions can be made in a protein sequence and nevertheless obtain a protein with like or other desired properties. Proteins with an amino acid sequence that is not 100% identical to that found in nature are termed analogues. An analogue optionally includes one or more amino acid substitutions, modifications, deletions, additions, or other change recognized in the art with the proviso that any such change produces a protein with the same type of activity (e.g. hydrolase) as the wild-type sequence. In making such changes, the hydropathic index, or the hydrophilicity of amino acids can be considered. In such changes, the substitution using amino acids whose hydropathic indices or hydrophilicity values are within ±2, those within ±1, and those within ±0.5 are optionally used.
Amino acid substitutions are optionally based on the relative similarity of the amino acid side-chain substituents, for example, their hydrophobicity, hydrophilicity, charge, size, and the like. Exemplary substitutions that take various of the foregoing characteristics into consideration are well known to those of skill in the art and include (original residue: exemplary substitution): (Ala: Gly, Ser), (Arg: Lys), (Asn: Gln, His), (Asp: Glu, Cys, Ser), (Gln: Asn), (Glu: Asp), (Gly: Ala), (His: Asn, Gln), (Ile: Leu, Val), (Leu: Ile, Val), (Lys: Arg), (Met: Leu, Tyr), (Ser: Thr), (Thr: Ser), (Tip: Tyr), (Tyr: Trp, Phe), and (Val: Ile, Leu). In particular, embodiments of the proteins can include analogues having about 50%, 60%, 70%, 80%, 90%, 95%, or 99% sequence identity to a wild-type protein.
It is further appreciated that the above characteristics are optionally taken into account when producing a protein with reduced or increased enzymatic activity. Illustratively, substitutions in a substrate binding site, exosite, cofactor binding site, catalytic site, or other site in a protein may alter the activity of the enzyme toward a substrate. In considering such substitutions the sequences of other known naturally occurring or non-naturally occurring like enzymes may be taken into account. Illustratively, a corresponding mutation to that of Asp213 in thermolysin is operable such as that done by Miki, Y, et al., Journal of Molecular Catalysis B: Enzymatic, 1996; 1:191-199. Optionally, a substitution in thermolysin of L144 such as to serine alone or along with substitutions of G8C/N60C/S65P are operable to increase the catalytic efficiency by 5-10 fold over the wild-type enzyme. Yasukawa, K, and Inouye, K, Biochimica et Biophysica Acta (BBA)—Proteins & Proteomics, 2007; 1774:1281-1288. The mutations in the bacterial neutral protease from Bacillus stearothermophilus of N116D, Q119R, D150E, and Q225R as well as other mutations similarly increase catalytic activity. De Kreig, A, et al., J. Biol. Chem., 2002; 277:15432-15438. De Kreig also teach several substitutions including multiple substitutions that either increase or decrease the catalytic activity of the protein. Id. and De Kreig, Eur J Biochem, 2001; 268(18):4985-4991. Other substitutions at these or other sites optionally similarly affect enzymatic activity. It is within the level of skill in the art and routine practice to undertake site directed mutagenesis and screen for subsequent protein activity such as by the methods of De Kreig, Eur J Biochem, 2001; 268(18):4985-4991.
A protein is optionally an analogue of a wild-type protein. An analogue of a protein has an amino acid sequence that when placed in similar conditions to a wild-type protein possess some level of the activity of a wild-type enzyme toward the same substrate. An analogue optionally has 500%, 250%, 200%, 150%, 110%, 99%, 98%, 97%, 96%, 95%, 94%, 93%, 92%, 91%, 90%, 85%, 80%, 75%, 70%, 60%, 50%, 25%, 10%, 5%, or any value or range of values therebetween, the activity of a wild-type protein. Any modification to a wild-type protein may be used to generate an analogue. Illustratively, amino acid substitutions, additions, deletions, cross-linking, removal or addition of disulfide bonds, or other modification to the sequence or any member of the sequence may be used to generate an analogue. An analogue is optionally a fusion protein that includes the sequences of two or more wild-type proteins, fragments thereof, or sequence analogues thereof.
Methods of screening for protein activity are known and standard in the art. Illustratively, screening for activity of an enzyme illustratively includes contacting an enzyme with a natural or synthetic substrate of an enzyme and measuring the enzymatic cleavage of the substrate. Illustrative substrates for this purpose include casein, which is cleaved by a protease to liberate folin-positive amino acids and peptides (calculated as tyrosine) that are readily measured by techniques known in the art. The synthetic substrate furylacryloylated tripeptide 3-(2-furylacryloyl)-L-glycyl-L-leucine-L-alanine obtained from Bachem AG, Bubendorf, Switzerland is similarly operable. Illustrative substrates of α-amylase include long chain carbohydrates such as amylose or amylopectin that make up starch. Other methods of screening for α-amylase activity include the colorimetric assay of Fischer and Stein, Biochem. Prep., 1961, 8, 27-33. It is appreciated that one of ordinary skill in the art can readily envision methods of screening for enzyme activity with the enzyme present in or on a variety of materials.
A protein is illustratively recombinant. Methods of cloning, synthesizing or otherwise obtaining nucleic acid sequences encoding a protein are known and standard in the art. Similarly, methods of cell transfection and protein expression are similarly known in the art and are applicable herein. Exemplary cDNA encoding the protein sequence of SEQ ID NO: 1 is the nucleotide sequence SEQ ID NO: 2. Exemplary cDNA encoding the protein sequence of SEQ ID NO: 3 is the nucleotide sequence found at accession number M11446 and SEQ ID NO: 4. Exemplary cDNA encoding the protein sequence of SEQ ID NO: 5 is the nucleotide sequence SEQ ID NO: 6
A protein may be coexpressed with associated tags, modifications, other proteins such as in a fusion protein, or other modifications or combinations recognized in the art. Illustrative tags include 6×His, FLAG, biotin, ubiquitin, SUMO, or other tag known in the art. A tag is illustratively cleavable such as by linking to protein via a target sequence that is cleavable by an enzyme known in the art illustratively including Factor Xa, thrombin, SUMOstar protein as obtainable from Lifesensors, Inc., Malvern, Pa., or trypsin. It is further appreciated that chemical cleavage is similarly operable with an appropriate cleavable linker.
Protein expression is illustratively accomplished following transcription of a protein nucleic acid sequence, translation of RNA transcribed from the protein nucleic acid sequence or analogues thereof. An analog of a nucleic acid sequence is any sequence that when translated to protein will produce a wild-type protein or an analogue of a wild-type protein. Protein expression is optionally performed in a cell based system such as in E. coli, Hela cells, or Chinese hamster ovary cells. It is appreciated that cell-free expression systems are similarly operable.
It is recognized that numerous analogues of protein are operable and within the scope of the present invention including amino acid substitutions, alterations, modifications, or other amino acid changes that increase, decrease, or not do alter the function of the protein sequence. Several post-translational modifications are similarly envisioned as within the scope of the present invention illustratively including incorporation of a non-naturally occurring amino acid, phosphorylation, glycosylation, addition of pendent groups such as biotin, avidin, fluorophores, lumiphores, radioactive groups, antigens, or other molecules.
A protein according to the invention is chemically modified by the addition of one or more polymeric moieties. Polymeric moieties optionally have a molecular weight ranging from 200 to 100,000 Daltons. Polymeric moieties are optionally linear, branched, liable, or combinations thereof. The polymeric moieties are optionally homomeric or heteromeric. Illustrative examples of polymeric moieties include one or more molecules of carbohydrate or polyoxyethylene (otherwise known as polyethylene glycol or “PEG”).
Illustrative examples of polymeric moieties include but are not limited to: polyalkyl alcohols and glycols (including heteroalkyl with, for example, oxygen) such as polyoxyethylenes and polyoxyethylene derivatives; dextrans including functionalized dextrans; styrene polymers; polyethylene and derivatives; polyanions including, but not limited to, polymers of heparin, polygalacturonic acid, mucin, nucleic acids and their analogs including those with modified ribosephosphate backbones, polypeptides of glutamate, aspartate, or combinations thereof, as well as carboxylic acid, phosphoric acid, and sulfonic acid derivatives of synthetic polymers; and polycations, including but not limited to, synthetic polycations based on acrylamide and 2-acrylamido-2 methylpropanetrimethylamine, poly(N-ethyl-4-vinylpyridine) or similar quarternized polypyridine, diethylaminoethyl polymers and dextran conjugates, polymyxin B sulfate, lipopolyamines, poly(allylamines) such as the strong polycation poly(dimethyldiallylammonium chloride), polyethyleneimine, polybrene, spermine, spermidine and proteins such as protamine, the histone polypeptides, polylysine, polyarginine and polyornithine; and mixtures and derivatives thereof. Suitable additional polymers are outlined in Roberts, M. J. et al. (2002) “Chemistry for peptide and protein PEGylation” Adv. Drug Deliv. Rev. 54, 459-476; Kinstler, O. et al. (2002) “Mono-N-terminal poly(ethylene glycol)-protein conjugates” Adv. Drug Deliv. Rev. 54; U.S. Application Ser. No. 60/360,722; U.S. Pat. No. 5,795,569; U.S. Pat. No. 5,766,581; EP 01064951; U.S. Pat. No. 6,340,742; WO 00176640; WO 002017; EP0822199A2; WO 0249673A2; U.S. Pat. No. 4,002,531; U.S. Pat. No. 5,183,550; U.S. Pat. No. 5,985,263; U.S. Pat. No. 5,990,237; U.S. Pat. No. 6,461,802; U.S. Pat. No. 6,495,659; U.S. Pat. No. 6,448,369; U.S. Pat. No. 6,437,025; U.S. Pat. No. 5,900,461; U.S. Pat. No. 6,413,507; U.S. Pat. No. 5,446,090; U.S. Pat. No. 5,672,662; U.S. Pat. No. 6,214,966; U.S. Pat. No. 6,258,351; U.S. Pat. No. 5,932,462; U.S. Pat. No. 5,919,455; U.S. Pat. No. 6,113,906; U.S. Pat. No. 5,985,236; WO 9428024A1; U.S. Pat. No. 6,340,742; U.S. Pat. No. 6,420,339; and WO 0187925A2.
Polyoxyethylene includes the generic structure —(CH2CH2O)n—, where n is an integer optionally from 2 to 2000. Optionally, n is an integer ranging from 50 to 500, optionally from 100 to 250, optionally from 150 to 250. Polyoxyethylene is optionally provided in a range of sizes attached to proteins using one or more of a variety of chemistries known in the art. Polyoxyelthylenes are optionally covalently associated with primary amines (e.g. lysine side chains or the protein N-terminus), thiols (cysteine residues), or histidines. Lysine occurs frequently on the surface of proteins, so binding of polyoxyethylene at lysine side chains produces a mix of reaction products. Since the pKa of the N-terminus is significantly different than the pKa of a typical lysine side chain, it is possible to specifically target the N-terminus for modification. Similarly, as most proteins contain very few free cysteine residues, cysteines (naturally occurring or engineered) may be targeted for site-specific interactions with polyoxyethylene.
Polyoxyethylene is optionally end capped where one end is end-capped with a relatively inactive group such as an alkoxy group, while the other end is a hydroxyl group that may be further modified by linker moieties. When the term “PEG” is used to describe polyoxyethylene the term “PEG” may be followed by a number (not being a subscript) that indicates a PEG moiety with the approximate molecular weight equal the number. Hence, “PEG10000” is a PEG moiety having an approximate molecular weight of 10,000 Daltons. The inventors have found that some embodiments including linear PEG10000 are superior to other PEG molecules.
The term “PEGylation” as used herein denotes modification of a protein by attachment of one or more PEG moieties via a linker at one or more amino acids. The polyoxyethylene (PEG) moiety is illustratively attached by nucleophilic substitution (acylation) on N-terminal α-amino groups or on lysine residue(s) on the gamma-positions, e.g., with PEG-succinimidyl esters. Optionally, polyoxyethylene moieties are attached by reductive alkylation—also on amino groups present in the protein using PEG-aldehyde reagents and a reducing agent, such as sodium cyanoborohydride. Optionally, polyoxyethylene moieties are attached to the side chain of an unpaired cysteine residue in a Michael addition reaction using for example PEG maleimide reagents. Polyoxyethylene moieties bound to a linker are optionally available from JenKem Technology USA, Allen, Tex. It is appreciated that any PEG molecule taught in U.S. Application Publication No: 2009/0306337 is operable herein. U.S. Application Publication No: 2009/0306337 also teaches methods of attaching PEG groups to a protein. PEG is optionally linked to a protein via an intermediate ester, amide, urethane, or other linkage dependent on the choice of PEG substrate and position of modification on a protein.
In some embodiments, a protein is an analogue of a hydrolase with the inclusion of additional cysteines to provide site specific incorporation sites for polyoxyethylene. In some embodiments, lysine or histidine residues are substituted with alternative amino acids that do not possess a target primary amine so as to prevent binding of a molecule of polyoxyethylene at that site. The choice of amino acid residues such as cysteines, lysines, or histidines to remove depends on the desired extent of modification. Optionally, simulation computer programs are used to predict whether modification with a polymer will interfere with the function of the protein as described in U.S. Pat. No. 7,642,340.
Proteins used in the inventions herein are optionally mono-substituted i.e. having only one polymeric moiety attached to a single amino acid residue in the protein molecule or to a N-terminal amino acid residue. Alternatively, two, three, four, or more polymeric moieties are present on a single protein. In embodiments where protein includes more than one polymeric moiety, it optionally has the same moiety attached to each associated amino acid group or to the N-terminal amino acid residue. However, the individual polymer groups may also vary from each other in size and length and differ between locations on the protein.
Reversible binding of one or more polymeric moieties at one or more sites on a protein is optionally used to regulate the rate of protein leeching from a coating composition upon immersion in or contact with water or other fluid. In these embodiments, the polymer is covalently attached but is liable upon exposure to weathering such as for example heating, water washing, or simply over time. The liable bond is optionally the bond between the protein and the polymer or within a linker present between a protein and a polymer.
An inventive process uses an inventive composition that includes one or more active chemically modified proteins incorporated into a base to form a coating material. The protein is optionally non-covalently associated and/or covalently attached to the base material or is otherwise associated therewith such as by bonding to the surface or by intermixing with the base material during manufacture such as to produce entrapped protein. In some embodiments, the protein is covalently attached to the base material either by direct covalent interaction between the protein and one or more components of the base material or by association via a linker.
There are several ways to associate protein with a base in a coating. One of which involves the application of covalent bonds. Specifically, free amine groups of the protein are optionally covalently bound to an active group of the base. Such active groups include alcohol, thiol, aldehyde, carboxylic acid, anhydride, epoxy, ester, or any combination thereof. This method of incorporating protein delivers unique advantages. First, the covalent bonds tether the proteins permanently to the base and thus place them as an integral part of the final composition with much less, if any at all, leakage of the protein. Second, the covalent bonds provide extended enzyme lifetime. Over time, proteins typically lose activity because of the unfolding of their polypeptide chains. Chemical binding such as covalent bonding effectively restricts such unfolding, and thus improves the protein life. The life of a protein is typically determined by comparing the amount of activity reduction of a protein that is free or being physically adsorbed with that of a protein covalently-immobilized over a period of time.
A protein is optionally associated with a base at a ratio of 1:1 to 1:20 (enzyme:base) by weight. Optionally, a protein is associated with a base at a ratio of 1:2 to 1:15, optionally 1:4 to 1:12 by weight.
Proteins are optionally uniformly dispersed throughout the substrate network to create a homogenous protein platform.
Chemical methods of protein attachment to materials will naturally vary depending on the functional groups present in the protein and in the material components. Many such methods exist. For example, methods of attaching proteins (such as enzymes) to other substances are described in O'Sullivan et al, Methods in Enzymology, 1981; 73:147-166 and Erlanger, Methods in Enzymology, 1980; 70:85-104.
Proteins are optionally present in a coating that is layered upon a substrate wherein the protein is optionally entrapped in the base material, admixed therewith, modified and integrated into the base material or layered upon a base material.
A water-stabilized coating composition optionally includes one or more additives, optionally for modifying the properties of the composition material. Illustrative examples of such additives include one or more light stabilizers such as a UV absorber or radical scavenger illustratively including those described in U.S. patent application Ser. No. 13/024,794 or U.S. Pat. No. 5,559,163, a plasticizer, a wetting agent, a preservative, a surfactant, a lubricant, a pigment, a filler, and an additive to increase sag resistance.
An inventive process optionally includes overlayering (coating) a substrate with a water-stabilized active temporary coating material such that the protein is capable of enzymatically degrading a component of a organic stain in contact with the active coating material. A substrate is any surface capable of being coated with an inventive coating. A substrate is optionally flexible or rigid with flexibility relative to that of a polyvinylchloride sheet with a thickness of 10 mm. A substrate has a first surface and a second surface wherein the first surface and the second surface are opposed. A coating is optionally overlayered on a substrate on a first surface, a second surface, both, or fully encapsulates a substrate. The coating of a substrate with a water-stabilized active coating material provides a self-cleaning surface that promotes the removal or loosening of an organic stain when present on the coating.
The identity of a substrate is limited only by its ability to be coated with an inventive composition. Illustratively, a substrate is metal, wood, natural or synthetic polymers such as fiberglass or other plastics, resins, paints, lacquers, stone, leather, other material, or combinations thereof. A substrate is optionally an automotive body panel or portion thereof. A substrate is optionally a boat hull or portion thereof. A substrate is optionally a wood floor or a coated wood floor. A substrate optionally includes a subcoating such as wood coated with a polyurethane protectant, or a subcoating is a paint, varnish, or other protectant commonly found on substrate. A water-stabilized temporary active coating material optionally contacts the substrate by overlaying the subcoating material.
Water-stabilized coatings according to embodiments of the present invention provide good adhesion to substrates, protection against environmental insults, protection against corrosion, and further provide active properties of the protein. Thus, in certain embodiments, coatings of water-stabilized active temporary material provide enzyme activity on a substrate useful in numerous applications such as detection of an analyte which is a substrate for the enzyme or a ligand for a receptor, antibody or lectin. In particular embodiments, coatings provide resistance against staining by enzyme digestion of one or more components of stain producing material.
When a water-stabilized composition is contacted with biological, food, or environmental material to produce an organic stain, the enzyme or combinations of enzymes contact the stain, or components thereof. The contacting allows the enzymatic activity of the protein to interact with and enzymatically alter components of the stain improving its removal from the substrate or coating.
Proteins are included in compositions according to embodiments of the present invention in amounts ranging from 0.1-50, 1-30, 1-20, 1-10, 2-8, 3-6, or other weight percent of the total weight of the material composition.
Enzyme containing coatings have a surface activity generally expressed in Units/cm2. Coatings including a thermolysin such as THERMOASE C160 (thermolysin from Bacillus stearothermophilus) optionally have functional surface activities prior to exposure to water of greater than 0.0075 Units/cm2. In some embodiments, thermolysin surface activity is between 0.0075 Units/cm2 and 0.05 Units/cm2 or any value or range therebetween. Optionally, thermolysin surface activity is between 0.0075 Units/cm2 and 0.1 Units/cm2 or any value or range therebetween. Optionally, thermolysin surface activity is between 0.01 Units/cm2 and 0.05 Units/cm2 or any value or range therebetween. In coatings containing α-amylase from Bacillis subtilis, typical surface activities prior to exposure to water are at or in excess of 0.01 Units/cm2. In some embodiments, α-amylase surface activity is between 0.01 Units/cm2 and 1.5 Units/cm2 or any value or range therebetween. Optionally, α-amylase surface activity is between 0.01 Units/cm2 and 2.5 Units/cm2 or any value or range therebetween. Optionally, α-amylase surface activity is between 0.01 Units/cm2 and 1.0 Units/cm2 or any value or range therebetween. In some embodiments, α-amylase surface activity is at or between 0.01 Units/cm2 and 4.0 Units/cm2. It is appreciated that higher surface activities are achievable by increasing the enzyme concentration, using enzyme with a higher specific activity such as an analogue of a wild-type enzyme, or by otherwise stabilizing enzyme activity during association with a base material.
It is appreciated that the inventive processes of facilitating stain removal will function at any temperature whereby the protein is active. Optionally, the inventive process is performed at 4° C. Optionally, an inventive process is performed at 25° C. Optionally, an inventive process is performed at ambient temperature. It is appreciated that the inventive process is optionally performed from 4° C. to 125° C., or any single temperature or range therein.
The presence of protein combined with the material of a substrate or a coating on a substrate, optionally, with water or other fluidic rinsing agent, breaks down stains for facilitated removal.
An inventive process includes providing a coating with an enzyme such that the enzyme is enzymatically active and capable to cleave or otherwise modify one or more components of an organic stain. In particular embodiments, an organic stain is based on organic matter such as that derived from an insect optionally an insect body, a fingerprint, foodstuffs, or from the environment.
An organic stain as defined herein is a stain, mark, or residue left behind after an organism, food, or environmental agent contacts a coating. An organic stain is not limited to marks or residue left behind after a coating is contacted by an insect body. Other sources of organic stains are illustratively: insect wings, legs, or other appendages; bird droppings; food or components of food; fingerprints or residue left behind after a coating is contacted by an organism; or other sources of organic stains such as bacteria or molecules present in water or soil.
Methods of preparing water-stabilized temporary active coating materials illustratively include association of aqueous solutions of protein and commercially available base materials by mixing such as by propeller mixing or hand mixing to a uniform or a non-uniform distribution of chemically modified protein to produce water-stabilized temporary coating materials.
Various aspects of the present invention are illustrated by the following non-limiting examples. The examples are for illustrative purposes and are not a limitation on any practice of the present invention. It will be understood that variations and modifications can be made without departing from the spirit and scope of the invention.
Example 1
Materials for Production of Water-Stabilized Active Temporary Coating Material
Materials: Freeze-dried crickets are purchased from PetSmart. Cricket bodies reportedly contain 58.3% protein. (D. Wang, et al., Entomologic Sinica, 2004; 11:275-283.) α-Amylase KLEISTASE SD80 from Bacillus subtilis (EC 3.2.1.1), lipase (lipase A12 (E.C.3.1.1.3) from Aspergillus niger), Protease N, Protease A, Protin SD AY-10, B. sterothermophilus TLP (THERMOASE C160), and THERMOASE GL30 (low activity preparation of B. sterothermophilus TLP) are obtained from AMANO Enzyme Inc. (Nagoya, JAPAN). Bovine serum albumin (BSA) from bovine serum, starch from potatoes, starch from wheat, maltose, sodium potassium tartrate, 3,5-dinitrosalicylic acid, Na2(PO4), NaCl, K2(PO4), casein, trichloroacetic acid, Folin & Ciocalteu's phenol reagent, Na2(CO3), sodium acetate, calcium acetate, tyrosine, p-nitrophenyl palmitate, ethanol, iodine, glucose, maltose, maltotriose, maltohexose, dextrin (10 kDa and 40 kDa) are obtained from Sigma Chemical Co., St. Louis, Mo., U.S.A. Aluminum panels and 8-path wet film applicator are purchased from Paul N. Gardner Company, Inc. (Pompano Beach, Fla.). Commercial base preparations: 1) TURTLE WAX carnauba car wax T-6 (carnauba wax containing silicone resin in petroleum distillates); 2) DURA SHINE as disclosed in U.S. Pat. No. 5,073,407; 3) PLASTX, a synthetic polymer auto polish; 4) TURTLE WAX ICE synthetic polish which is a synthetic blend of hydrocarbons and silicon resins; 5) EAGLE ONE NANOWAX described in U.S. Pat. No. 7,503,963; 6) NU FINISH NF-76 as described in U.S. Pat. No. 7,067,573; and 7) TURTLE WAX PLATINUM series wax which is a blend of brazilian carnauba with bavarian montan wax along with light reflective polymers, are purchased from a local auto parts supplier. An Oster blender (600 watts) and light mayonnaise are obtained from a local supermarket. Freeze-dried crickets are obtained from Fluker Laboratories (Port Allen, La.). Polyethylene glycol (PEG) derivatives with succinimidyl ester of different molecular weights are obtained from Fishersci (Pittsburgh, Pa.).
Example 2
Preparation of Enzymes
Lipase, α-amylase, and thermolysin are each prepared by ultrafiltration from raw powder. For α-amylase, a 150 mL solution from raw powder (6.75 g) is prepared in DI water. For thermolysin, a 150 mL solution of 1.5 g B. sterothermophilus thermolysin-like-protease (TLP) is prepared in DI water. For lipase, a 150 mL solution of 1.5 g lipase A12 is prepared in DI water. The insoluble large impurity in raw powder is removed by filtration over a 200 nm PTFE filter. The obtained solution has a protein concentration of 20 mg/mL (measured by the Bradford method) and is maintained on ice.
Ultrafiltration is performed using a 150 mL Amicon cell (cooled with ice) with a pressure of 55 psi and an ultrafiltration membrane with a cut-off of 30 kDa from Millipore (Billerica, Mass.). Ultrafiltration is repeated 3 times by refilling the cell back to 150 mL of DI water after each run. The final remaining purified protein solution is quantified by the Bradford method and diluted to the final working concentration used for chemical modification and production of coating materials. Coatings are made using a solution of 50, 100, 200, or 300 mg/mL of purified enzyme following chemical modification.
Example 3
PEGylation of enzyme. Purified enzyme (c′-amylase, thermolysin, or lipase) is mixed with PEG (monofunctional linear PEG10000, PEG12000, PEG20000, or combinations) derivatized with succinimidyl ester at a mole ratio of 1:5 enzyme:PEG in 0.05 M sodium phosphate buffer pH 7.5, and subjected to mild agitation by shaking at 200 rpm at room temperature for 60 minutes. Some preparations further involve isolation of non-reacted PEG by filtration with a filter with an appropriate molecular weight cut-off for each PEG used in the PEGylation reactions.
Example 4
Commercial wax formulations 1-7 of Example 1 and a concentrated PEGylated-enzyme (200 mg/ml prior to PEGylation used without subsequent removal of unreacted PEG) are vigorously mixed by vortexing at 1000 rpm at a ratio of from 4:1 to 12:1 base:enzyme by weight (unmodified enzyme) to form water-stabilized temporary coating material preparations using the base compositions of Example 1. Exemplary preparations are those of A through KKK as depicted in Table 1.












TABLE 1







Preparation
Base
Enzyme
Ratio (B:E)









A
1
α-amylase
4:1



B
1
α-amylase
8:1



C
1
α-amylase
12:1 



D
1
thermolysin
4:1



E
1
thermolysin
8:1



F
1
thermolysin
12:1 



G
1
lipase
4:1



H
1
lipase
8:1



I
1
lipase
12:1 



J
2
α-amylase
4:1



K
2
α-amylase
8:1



L
2
α-amylase
12:1 



M
2
thermolysin
4:1



N
2
thermolysin
8:1



O
2
thermolysin
12:1 



P
2
lipase
4:1



Q
2
lipase
8:1



R
2
lipase
12:1 



S
3
α-amylase
4:1



T
3
α-amylase
8:1



U
3
α-amylase
12:1 



V
3
thermolysin
4:1



W
3
thermolysin
8:1



X
3
thermolysin
12:1 



Y
3
lipase
4:1



Z
3
lipase
8:1



AA
3
lipase
12:1 



BB
4
α-amylase
4:1



CC
4
α-amylase
8:1



DD
4
α-amylase
12:1 



EE
4
thermolysin
4:1



FF
4
thermolysin
8:1



GG
4
thermolysin
12:1 



HH
4
lipase
4:1



II
4
lipase
8:1



JJ
4
lipase
12:1 



KK
5
α-amylase
4:1



LL
5
α-amylase
8:1



MM
5
α-amylase
12:1 



NN
5
thermolysin
4:1



OO
5
thermolysin
8:1



PP
5
thermolysin
12:1 



QQ
5
lipase
4:1



RR
5
lipase
8:1



SS
5
lipase
12:1 



TT
6
α-amylase
4:1



UU
6
α-amylase
8:1



VV
6
α-amylase
12:1 



WW
6
thermolysin
4:1



XX
6
thermolysin
8:1



YY
6
thermolysin
12:1 



ZZ
6
lipase
4:1



AAA
6
lipase
8:1



BBB
6
lipase
12:1 



CCC
7
α-amylase
4:1



DDD
7
α-amylase
8:1



EEE
7
α-amylase
12:1 



FFF
7
thermolysin
4:1



GGG
7
thermolysin
8:1



HHH
7
thermolysin
12:1 



III
7
lipase
4:1



JJJ
7
lipase
8:1



KKK
7
lipase
12:1 










The preparations of Table 1 are applied to various substrates by spreading with a cloth and optionally buffed as per the manufacturer's directions for each base preparation. Base formulation with free (unmodified) enzyme and base with unreacted PEG (no enzyme) are used as controls at the same conditions. Each material is spread on various surfaces including glass plates, quartz plates, plastics, aluminum panels. When indicated by manufacturer of base, the coating is incubated for a “drying” period of 3 minutes followed by surface polishing with paper towel by hand.
Each coating contains a uniform distribution of enzyme. Test panels coated with preparations A-KKK are subjected to fluorescence microscopy to determine the uniformity of enzyme distribution. Test panels are coated with coatings containing unmodified enzyme as a control (e.g. α-amylase non-chemically modified) or PEGylated enzyme. The intrinsic fluorescence of enzyme is used to detect the enzyme distribution. Coatings are applied to glass microslides and covered with a glass microslip and are subjected to light of wavelength 488 nm. Fluorescence is detected at 570 nm using exposures of 5.313 seconds with a gain of 2. FIG. 3 depicts the distribution of preparation A at a magnification of 200× indicating even distribution of enzyme in both preparations. Similar results are observed using preparations B-KKK.
Example 5
Weathering Durability of Coatings
Coated aluminum panels are cut to test size of 1.2 cm×1.9 cm and subjected to weather by submersion in room temperature DI water for 0 minutes, 30 minutes, or 90 minutes with agitation. The test panels are removed and rinsed with flowing DI water for 20 seconds followed by assay for remaining enzyme activity.
Test panels coated with α-amylase containing coatings are assayed by determination of amydolytic activity by reacting test panels with the α-amylase substrate 1% w/v potato starch in 20 mM sodium phosphate buffer with 6.7 mM sodium chloride (pH 6.9). The substrate solution (2 mL) is added to one rectangular piece of the coated test panel (1.2 cm×1.9 cm) and incubated for 3 min at 25° C. The equivalent amount of reducing sugar produced is determined using a UV-VIS spectrometer (Cary 300-Varian Inc., Walnut Creek, Calif., USA) at 540 nm. One unit of α-amylase activity is defined as 1.0 mg of reducing sugar (calculated from a standard curve previously calibrated against maltose) released from starch in 3 min at room temperature.
Coatings prepared with thermolysin are assayed for proteolytic surface activity essentially following the method of Folin and Ciocalteau, J. Biol. Chem., 1927; 73: 627-50. Briefly, 1 mL of 2% (w/v) casein in sodium phosphate (0.05 M; pH 7.5) buffer solution is used as substrate together with 200 μl of sodium acetate, 5 mM calcium acetate (10 mM; pH 7.5). The substrate solution is pre-incubated in a water bath for 3 min to reach 37° C. The reaction is started by adding one piece of sample plate coated with B. sterothermophilus TLP based coating (1.2×1.9 cm) followed by shaking for 10 min at 200 rpm at which time the reaction is stopped by adding 1 ml of 110 mM trichloroacetic acid (TCA) solution. The mixture is incubated for 30 min at 37° C. prior to centrifugation. The equivalent of tyrosine in 400 μL of the TCA-soluble fraction is determined at 660 nm using 200 μL of 25% (v/v) Folin-Ciocalteau reagent and 1 mL 0.5 M sodium carbonate. One unit of activity is defined as the amount of enzyme hydrolyzing casein to produce absorbance equivalent to 1.0 μmol of tyrosine per minute at 37° C. This result is converted to Units/cm2.
An exemplary residual activity depicting the water-stability of coating preparations is depicted in FIG. 4A using preparation A (Table 1) compared to unmodified enzyme (free enzyme). Preparation A with PEGylated-enzyme shows approximately 70% and 30% activity at 30 minutes and 90 minutes, respectively, relative to coatings not subjected to water washing. The coatings containing unmodified enzyme (free enzyme) demonstrate less than 10% and 1.5% remaining after 30 and 90 minutes water washing, respectively, relative to no water washing. This level of water-stability is also observed using preparations B-KKK of Table 1. Overall, these results indicate that coatings containing chemically modified enzyme are stable against water washing compared to coatings containing unmodified enzyme.
Water durability of PEGylated enzyme containing coatings is also observed by retention of water contact angle. Water contact angle is used as a measure of the hydrophobicity or hydrophilicity of a surface such as a coating. A highly hydrophilic surface will hold a water droplet with a contact angle typically of 0 degrees to 30 degrees. A highly hydrophobic surface will show contact angles of 90 degrees or more. Changes in water contact angle represent changes in the chemical makeup of a surface or a coating. Water contact angle is measured on flat coated glass plates using a goniometer using the sessile drop method. As depicted in FIG. 4B, the absence of modification of an enzyme results in nearly complete leeching of enzyme from the coating producing a reduction in water contact angle. In contrast, the water contact angle is maintained to a level lower than that for enzyme free base material coatings following the same water submersion conditions with estimated 46% enzyme-PEG retention. (FIG. 4B.)
Example 6
Preparation of organic stains and application to coated substrate and self-cleaning activity of coating preparations. For preparation of insect matter, 60 g of Freeze-dried crickets are chopped into powder by a blender (Oster, 600 watt) for 10 min. The stain solution is prepared by vigorously mixing 2 g of cricket powder with 6 mL of DI water. A template of uniform spacing is used to apply the stain on the coating surface. The cricket stains are dried at 40° C. for 5 min. Each test panel is placed into a glass dish subjected to rinsing with 200 mL of DI water under 300 rpm shaking at RT for various times. The time of the stain removal is recorded. In order to reduce random error, the time of the first and last drop removed are not included. The average rinsing time of eight stain spots is averaged for stain removal time. Test panels coated with PEGylated thermolysin containing coatings provide improved stain removal by gentle rinsing as compared to panels coated with base material alone.
Amylase containing coatings of Table 1 are placed on the plastic surfaces of standard compact disks or aluminum test panels as in Example 4. A 0.3 g sample of light mayonnaise is placed on various sections of the test panels followed by air dry at ambient conditions for 2 minutes prior to standing upright. Light mayonnaise includes large macromolecules such as fat and starch that contribute to its high viscosity and thus to the high frictional force on the coating surface that prevents gravity driven sliding of the mayonnaise when the test panel is tilted vertically. Coatings containing modified α-amylase catalyze the hydrolysis of the emulsifier resulting in tremendously lowered viscosity as a consequence of a phase separation at the stain-coating interface, thus allowing the stain to slide down the test panel when tilted vertically.
Similarly, aluminum test panels are coated with preparation A of Table 1, preparation A with PEG and no enzyme, or a coating with no enzyme or PEG as a control. As depicted in FIG. 5, the test panels coated with enzyme free coating (I) or PEG-coating (II) show no movement of the light mayonnaise following tilting to a vertical position. The coating with PEGylated enzyme, however, shows significant self-cleaning remaining. (FIG. 5.)
The coatings of Table 1 containing PEGylated lipase are used to test removal of fingerprints from glass or transparent plastic surfaces. The self-cleaning of fingerprints by PEGylated lipase containing preparations is tested at enzyme dilutions of 1:6 (I) and 1:12 (II) (Example 4) on glass substrates. Test panels are coated with either PEGylated lipase containing base materials or control materials (no enzyme) and incubated at room temperature 24 hours. The test panels are stained with human fingerprints or facial skin contact. The coated test panels are then incubated in an oven at 120° C. for 1 to 6 hours. For better visualization of the remaining finger prints, coatings are washed under running DI water (50 mL/sec) for 1 minute and dried using air. Prior to heating each coating is subjected to the same level of staining by fingerprints. Following baking, coatings without enzyme show significant residual staining while coatings containing PEGylated lipase show greatly reduced stain remaining with the level of residual fingerprint staining reduced with increased enzyme concentration.
Various modifications of the present invention, in addition to those shown and described herein, will be apparent to those skilled in the art of the above description. Such modifications are also intended to fall within the scope of the appended claims.
It is appreciated that all reagents are obtainable by sources known in the art unless otherwise specified or synthesized by one of ordinary skill in the art without undue experimentation. Methods of protein production and purification are similarly within the level of skill in the art.
REFERENCE LIST


    Harris, J. M. and Kozlowski, A. (2001). Improvements in protein PEGylation: pegylated interferons for treatment of hepatitis C. J. Control Release 72, 217-224.
    Veronese, F. and Harris, J. M. Eds. (2002). Peptide and protein PEGylation. Advanced Drug Delivery Review 54(4), 453-609.
    Veronese, F. M.; Pasut, G. (2005), PEGylation, successful approach to drug delivery, Drug Discovery Today 10 (21): 1451-1458
    Veronese, F. M.; Harris, J. M. (2002), Introduction and overview of peptide and protein pegylation, Advanced Drug Delivery Reviews 54 (4): 453-456
    Damodaran V. B.; Fee C. J. (2010), Protein PEGylation: An overview of chemistry and process considerations, European Pharmaceutical Review 15 (1): 18-26
    Harris, J. M.; Chess, R. B. (2003), Effect of pegylation on pharmaceuticals. Nature Reviews Drug Discovery 2, 214-221
    Rodriguez-Martinez J. A., et. al. (2008) Stabilization of a-Chymotrypsin Upon PEGylation. Correlates With Reduced Structural Dynamics. Biotechnology and Bioengineering, 101, 1142-1149
    Li, J.; Kao, W. J. (2003), Synthesis of Polyethylene Glycol (PEG) Derivatives and PEGylated-Peptide Biopolymer Conjugates. Biomacromolecules 4, 1055-1067


The sequence listings submitted on the original filing date of Apr. 29, 2011, and resubmitted on Feb. 7, 2012 as the file entitled “TTC-96202-ST25” created on Mar. 25, 2011, and having a file size of 19.3 KB, and the file entitled “TTC-96202-Sequence” created on Apr. 28, 2011, and having a file size of 44.2 KB, are hereby incorporated by reference.
Patents and publications mentioned in the specification are indicative of the levels of those skilled in the art to which the invention pertains. These patents and publications are incorporated herein by reference to the same extent as if each individual patent or publication was specifically and individually incorporated herein by reference.
The foregoing description is illustrative of particular embodiments of the invention, but is not meant to be a limitation upon the practice thereof. The following claims, including all equivalents thereof, are intended to define the scope of the invention.

## Claims

1. An enzymatic cleaning composition comprising:
a base material selected from the group consisting of paraffin wax, microcrystalline wax, petroleum wax, carnuba wax, candeilla vegetable wax, montan coal derived wax, dimethylsilicones, and aminofunctional silicones; a first polyoxyethylene covalently bound to an enzyme; and a second polyoxyethylene which is unmodified.

2. The composition of claim 1 wherein said base and said enzyme are present in a ratio of 2:1 to 20:1 by weight respectively.

3. The composition of claim 1 wherein said enzyme is a hydrolase.

4. The composition of claim 3 wherein said hydrolase is a bacterial neutral thermolysin-like-protease, an amylase, or a lipase.

5. The composition of claim 1 wherein said first polyoxyethylene has a molecular weight between 1,000 and 15,000 Daltons.

6. The composition of claim 1 wherein said first polyoxyethylene and said second polyoxyethylene have equal monomers of oxyethylene.

7. The composition of claim 1 wherein said first polyoxyethylene is derivatized with a succininimidyl ester.

8. The composition of claim 1 wherein said second polyoxyethylene is covalently attached to said enzyme via an intermediate urethane linkage.

9. The composition of claim 1 wherein said second polyoxyethylene is directly or indirectly covalently bound to an amino group on said enzyme.

10. The composition of claim 1 wherein said second polyoxyethylene is directly or indirectly covalently bound to a cysteine within said enzyme.

11. The composition of claim 1 wherein said first polyoxyethylene is a branched molecule.