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2011_June_Progress Report 1

PROGRESS REPORT: JUNE 2011 

BIODEGRADATION TESTING SUPPLEMENTAL ENVIRONMENTAL PROJECT 

Submitted to 

E.I du Pont de Nemours and Company 

Wilmington, Delaware 19898 

U.S.A. 

United States Environmental Protection Agency 

Washington, DC 

U.S.A. 

Wildlife International, Ltd.

8598 Commerce Drive 

Easton, Maryland 21601 USA 

1 (410) 822-8600 

June 29, 2011 

Page 1 of 6 
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2011_March _Progress Report 2 

Table of Contents 

Title Page ...................................................................................................................................1 

Table of Contents.......................................................................................................................2 

Introduction................................................................................................................................3 

Progress Summary .....................................................................................................................3 

Appendix A. Invoice.................................................................................................................4 

Appendix B. Task E: Modifications and Refinements to Test Methods .................................6 
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2011_March _Progress Report 3 

Introduction 

This document presents the progress of the technical work for the Biodegradation Testing 

Supplemental Environmental Project (“Biodegradation SEP”) performed from 28 May 2011 

up to 24 June 2011. Studies conducted under protocol will be examined by the Wildlife 

International Quality Assurance Unit for compliance with Good Laboratory Practices, 

Standard Operating Procedures and the specified protocol. Raw data that has been audited 

by the Wildlife International Quality Assurance will be included in the progress report, as 

appropriate. 

Progress Summary 

The total costs associated with the project for the period covered in this Progress Report are 

approximately $3,040 for labor and $18,900 for instrumentation. The invoice and an 

allocation of costs with associated tasks are presented in Appendix A. 

Work conducted over the period covered by this progress report consisted of the review and 

discussion of data from test substance characterization and discussion of Task E items with 

stakeholders. Work also included on-going revisions of draft method validation protocols, 

and preparation of a Scope of Work to evaluate the analysis of 6-2 FTOH, 8-2 FTOH and 

10-2 FTOH in MTBE extracts of an activated sludge matrix by liquid chromatography mass 

spectrometry in the presence and absence of the Group A1 polymer (Appendix B). 
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2011_March _Progress Report 4 

Appendix A 

Invoice 
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2011_June_Progress Report 

Allocation of Costs 

Month, Year Tasks 

 Design and 

Procurement of 

SCAS Units 

(Task A) 

Acquisition 

of Standards 

(Task B) 

Adaptation of 

Analytical 

Method (Task C) 

Pilot Studies 

Task (D) 

Refinement 

of Methods 

Task (E) 

Method 

Validations 

(Task F) 

Storage 

Stability 

(Task G) 

Biod

dat

Stu

(Tas

Dec 2009 $1,803 $1,085 $42,422 Jan 2010 $29,227 Feb 2010 $1,300 $36,745 Mar 2010 $14,350 Apr 2010 $570 $15,935 May 2010 $760 $71,860 June 2010 $57,870 $35,003 July 2010 $42,430 $15,130 August 2010 $32,530 $113,718 September 2010 $1,350 $115,228 October 2010 $88,823 $3,613 November 2010 $42,690 $855 $570 December 2010 $18,385 $3,530 $3040 January 2011 $15,570 February 2011 $2,945 March 2011 $8,075 April 2011 $9,820 May 2011 $5,770 June 2011 $2,850 $190 Labor Total $1,803 $3,715 $344,719 $428,988 $53,028 $3,800 Materials $6,800 $10,500 $20,644 $1,724 Total Cost $8,603 $14,215 $365,363 $430,712 $53,208 $3,800 
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2011_June_Progress Report 6

Appendix B. Task E: Modifications and Refinements to Test Methods