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Agricultural Marketing Service

Federal Grain Inspection Service

Biotechnology Proficiency Program

October 2017 Bi-Annual Report
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USDA/AMS/FGIS Proficiency Program

Testing for the Presence of Biotechnology-derived Events in Corn and Soybeans

October 2017 Sample Distribution Results

Purpose of USDA/AMS/FGIS Proficiency Program

Through the USDA/AMS/FGIS Corn and Soy Proficiency Program, USDA seeks to 

improve the overall performance of testing for biotechnology-derived grains and oil seeds. 

The USDA/AMS/FGIS Proficiency Program helps organizations identify areas of concern 

and take corrective actions to improve testing precision, capability and reliability.

Program Description and Assessment of Results

The USDA/AMS/FGIS proficiency program report contains inter-laboratory comparisons 

for the purpose of proficiency testing (i.e. to determine the performance of individual 

laboratories’ ability to detect and/or quantify biotechnology-derived traits in corn or soy as 

well as to monitor laboratories’ continuing performance). The program does not assess 

the effectiveness of different detection methods for biotechnology-derived traits, nor does 

it determine the characteristics of fortified samples to a particular degree of accuracy.

In this round of the USDA/AMS/FGIS Proficiency Program sample distribution, one set of 

samples was used for both qualitative and quantitative analyses. The samples were 

fortified with various combinations and concentrations of biotechnology-derived traits, and 

participants had the choice of providing qualitative and/or quantitative results. Qualitative 

results were scored by computing the “percentage of correctly reported biotechnologyderived traits” in the samples (Tables 1 to 45 and Figure 1 for DNA-based analysis, and 

Tables 44 to 53 for protein-based analysis). The “percentage false positive” and 

“percentage false negative” were calculated by dividing the number of incorrectly reported 

results by the number of “provided negatives” or “provided positives” that were distributed 

to the participants. It should be noted that trait-specific, DNA-based testing can discern 

between different traits that express the same protein, (e.g. Roundup ReadyTM (RUR) and 

Roundup ReadyTM II (RUR II)), whereas in most instances, construct-specific DNA-based 

testing or protein-based testing cannot.

Consensus mean values were calculated on the sets of quantitative data shown in Tables 

54 to 74, with outliers excluded. Data sets were treated equally with this model, assuming 

no laboratory effect on outcomes, and are most likely idealistic. To assess accuracy of 

individual participant’s submitted quantitative results for a specified biotechnologyderived event, z-scores (based on: (reported value – consensus mean value) / standard 

deviation) were computed for each reported quantification result (Tables 54 to 74). Tests 

for outliers and z-scores assume a normal distribution. At the 0.0 or 0.1% fortification 

levels, and on tables with a limited number of results, the distributions are not likely normal 

and are probably skewed. A false positive on a 0.0% spike level is considered an outlier. 

At the 0.1% fortification level, outlier tests will likely identify more outliers than should be 

declared. Some judgment will be necessary when interpreting data at these low 

levels. Absolute values for z-scores that are > 2 should be scrutinized by the participating 

lab. Those that are > 3, or classified as outliers, are clearly suspect and action should be 

taken by the participating laboratory. Prior to computing the z-scores, outliers in the 

distribution of values were eliminated by use of the “Grubb’s Test for Outliers.” To 
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evaluate the performance as a group (i.e., inter-laboratory variation), a summary table 

(Table 75) was prepared to show the accuracy and precision of compiled quantification 

results at each fortification level for the various biotechnology-derived events.

Sample Preparation and Composition

USDA/AMS/FGIS receives 100% Biotechnology-derived event trait material from life 

science companies through materials transfer agreements. Specific GE trait materials 

are characterized at USDA/AMS/FGIS and tested for the presence of all other available 

traits (also used in the program) by event-specific polymerase chain reaction (PCR) 

testing. Occasionally, stack-traits are identified. Seed purity and zygosity characteristics 

are not assessed by USDA/AMS/FGIS. Commercially available and internationally 

recognized reference material are purchased through the Institute for Reference Materials 

and Measurements (IRMM) or the American Oil Chemists Society (AOCS) as finely 

ground flour, containing a certified percentage weight/weight (%w/w), level. 

USDA/AMS/FGIS uses commercially available reference standards to characterize the 

composition of proficiency samples prior to disseminating to participants

Biotechnology-derived event-free base material used for fortified sample preparation 

included both Pioneer Negative Corn (PNC), provided by Pioneer Hi-Bred International, 

Inc., and Popcorn provided by Amish Country Popcorn. The maize is ground to the 

consistency of fine flour using a high speed rotor mill, "Pulverisette 14.” A 50 gram aliquot 

of 100% event material (corn or soy) is ground in liquid nitrogen, in a highly controlled 

environment using a SPEX Certi Prep 6800 Freezer Mill. The 100% event sample is 

stored at -20 oC until further use. The 100% event material is blended gravimetrically with

well characterized non-event maize to a specified %w/w concentration. The sample sets 

are then analyzed by real-time PCR using USDA/AMS/FGIS in-house validated methods

The corn samples contained various combinations and concentrations of the following 

biotechnology-derived traits: MON810, GA21, NK603, Herculex® (TC1507), MON863, 

Herculex® RW (DAS-59122-7), MIR604, Event 3272, MON 88017, MON 89034, and MIR 

162. The soybean samples contained various combinations and concentrations of the 

following biotechnology-derived traits: the biotechnology-derived glyphosate-tolerant 

soybeans (Roundup ReadyTM), the glufosinate ammonium tolerant soybeans (A2704-12), 

the biotechnology-derived glyphosate-tolerant soybeans (Roundup ReadyTM II), and high 

oleic acid soybeans (DP305423). The various biotechnology-derived event concentration 

levels were produced on a %w/w basis. A calculated amount of ground biotechnologyderived corn or soybeans was blended to homogeneity with a calculated amount of 

ground non-biotechnology-derived corn or soybeans to produce concentrations ranging 

from 0.0 to 2.0% of a specified event. Each participant received four corn and two

soybean samples. Individual samples contained approximately 5 grams of ground 

material.

Samples prepared at a particular %w/w fortification should in theory be concordant with 

consensus values as cited in the report. In many instances, however, the %w/w 

fortification value did not agree with analytical data generated by PCR when compared to 

commercially available reference standards using in-house validated methods. The trend 

of generating lower reported concentrations compared with gravimetric fortified values

can have several possible explanations, including that of differences in trait zygosity 

between material used in the corn and soybean samples compared with the commercially 
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available reference materials. For example, USDA/AMS/FGIS in-house validated 

methods yield composite averages for MON810 to be about half the %w/w fortified levels; 

conversely, Herculex® RW composite averages were observed to be higher than the 

%w/w fortifications. USDA/AMS/FGIS data are consistent with historical data generated 

by USDA/AMS/FGIS proficiency program participants. For this reason, consensus 

means are used for statistical evaluation in lieu of gravimetric fortification values.

Program Participants

Participants included organizations from Africa, Asia, Europe, North America, and South 

America. Each participant received a study description and a data report form by 

electronic mail, and included with the samples. Participants submitted results by 

electronic mail. No analytical methodologies were specified, and organizations used both 

DNA and protein-based testing technologies. One hundred and five (105) organizations 

received samples in the October 2017 round of proficiency testing, and hundred (100)

organizations submitted results to USDA/AMS/FGIS.

• Forty-eight (48) participants submitted qualitative results only (of these 48, four

(4) participants performed a combination of DNA and protein based testing), 

• Six (6) submitted quantitative results only (of these 6, two (2) participants

performed a combination of DNA and protein based testing),

• Forty-seven (47) participants submitted a combination of qualitative and 

quantitative results (of these 47, two (2) participants performed a combination 

of DNA and protein based testing), and 

• Eight (8) participants submitted protein based results, using Lateral Flow Strip 

(LFS) and/or Enzyme-linked Immunosorbent Assay (ELISA) qualitative 

analyses (of these 8, one (1) participant performed Enzyme-linked 

Immunosorbent Assay (ELISA) quantitative analysis and one (1) participant 

performed Lateral Flow Strip (LFS) quantitative analysis.

In this report, participating organizations are identified by a confidential “Participant 

Identification Number.” Appendix I identifies those organizations who gave 

USDA/AMS/FGIS permission to list them as participants in the USDA/AMS/FGIS

Proficiency Program; several listed organizations requested that their identity remain 

anonymous.

Data Summary Results

Data submitted by the participants is summarized in this report primarily in tables and 

figures. Participants reported their results on a qualitative basis, quantitative basis, or a 

combination of both qualitative and quantitative bases. Qualitative results were reported 

as either positive (presence) or negative (absence) for a particular event in each sample. 

Quantitative results were reported as the concentration (% w/w) of a particular event in 

the sample. Due to the complexity of the data, this report summarizes the data as follows:

Qualitative Data Summaries: This section summarizes qualitative sample analysis 

data:
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• Table 1: Qualitative results of corn fortified with 35S for all participants (DNAbased assays).

• Table 2: Percentages of correct results, false negatives, and false positives in 

qualitative reports of 35S for all participants.

• Table 3: Qualitative results of corn fortified with NOS for all participants (DNAbased assays).

• Table 4: Percentages of correct results, false negatives, and false positives in 

qualitative reports of NOS for all participants.

• Table 5: Qualitative results of corn fortified with FMV for all participants (DNAbased assays).

• Table 6: Percentages of correct results, false negatives, and false positives in 

qualitative reports of FMV for all participants.

• Table 7: Qualitative results of corn fortified with T25 for all participants (DNAbased assays).

• Table 8: Percentages of correct results, false negatives, and false positives in 

qualitative reports of T25 for all participants.

• Table 9: Qualitative results of corn fortified MON810 with for all participants (DNAbased assays).

• Table 10: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON810 for all participants.

• Table 11: Qualitative results of corn fortified GA21 with for all participants (DNAbased assays).

• Table 12: Percentages of correct results, false negatives, and false positives in 

qualitative reports of GA21 for all participants.

• Table 13: Qualitative results of corn fortified NK603 with for all participants (DNAbased assays).

• Table 14: Percentages of correct results, false negatives, and false positives in 

qualitative reports of NK603 for all participants.

• Table 15: Qualitative results of corn fortified with Herculex® for all participants 

(DNA-based assays).

• Table 16: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Herculex® for all participants.

• Table 17: Qualitative results of corn fortified with MON863 for all participants (DNAbased assays).

• Table 18: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON863 for all participants.

• Table 19: Qualitative results of corn fortified with Herculex® RW for all participants 

(DNA-based assays).

• Table 20: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Herculex® RW for all participants.

• Table 21: Qualitative results of corn fortified with MIR604 for all participants (DNAbased assays).

• Table 22: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MIR604 for all participants.
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• Table 23: Qualitative results of corn fortified with Event 3272 for all participants 

(DNA-based assays).

• Table 24: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Event 3272 for all participants.

• Table 25: Qualitative results of corn fortified with MON 88017 for all participants 

(DNA-based assays).

• Table 26: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON 88017 for all participants.

• Table 27: Qualitative results of corn fortified with MON 89034 for all participants 

(DNA-based assays).

• Table 28: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON 89034 for all participants.

• Table 29: Qualitative results of corn fortified with MIR162 for all participants (DNAbased assays)

• Table 30: Percentages of correct results, false negatives and false positives in 

qualitative reports of MIR162 for all participants.

• Table 31: Qualitative results of soybeans fortified with CP4 EPSPS (Roundup 

ReadyTM) for all participants (DNA-based assays).

• Table 32: Percentages of correct results, false negatives, and false positives in 

qualitative reports of CP4 EPSPS for all participants.

• Table 33: Qualitative results of soybeans fortified with A2704-12 (Liberty Link®) for 

all participants (DNA-based assays). 

• Table 34: Percentages of correct results, false negatives, and false positives in 

qualitative reports of A2704-12 for all participants.

• Table 35: Qualitative results of soybeans fortified with CP4 EPSPS (Roundup 

ReadyTM II) for all participants (DNA-based assays).

• Table 36: Percentages of correct results, false negatives, and false positives in 

qualitative reports of CP4 EPSPS (Roundup ReadyTM II) for all 

participants.

• Table 37: Qualitative results of soybeans fortified with DP305423 for all participants 

(DNA-based assays).

• Table 38: Percentages of correct results, false negatives, and false positives in 

qualitative reports of DP305423 for all participants.

• Table 39: Qualitative results for soybeans fortified with 35S for all participants 

(DNA-based assays).

• Table 40: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Soy 35S for all participants.

• Table 41: Qualitative results for soybeans fortified with NOS for all participants 

(DNA-based assays).

• Table 42: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Soy NOS for all participants.

• Table 43: Qualitative results of soybeans fortified with FMV for all participants 

(DNA-based assays).

• Table 44: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Soy FMV for all participants.
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• Table 45: Composite percentages of correct results, false negatives, and false 

positives in qualitative reports for each biotechnology-derived event for all 

participants (DNA-based assays).

• Figure 1: Group average of percentage correct for Qualitative reports on each 

event (DNA-based assays).

Protein Based Data Summaries: This section summarizes protein based sample 

analysis data:

• Table 46: Lateral Flow Strip (LFS) testing results for the detection of biotechnologyderived events in corn.

• Table 47: Percentage of correct results, false negatives, and false positives in 

qualitative reports for biotechnology-derived events in corn using Lateral 

Flow Strip (LFS) testing. 

• Table 48: Results for soybeans fortified with CP4EPSPS and A2704-12 for 

participants using Lateral Flow Strip (LFS) testing.

• Table 49: Percentage of correct results in qualitative reports for CP4EPSPS and 

A2704-12 for participants using Lateral Flow Strip (LFS) testing.

• Table 50: Results for the detection of biotechnology-derived events in corn using 

Enzyme-Linked Immunosorbent Assay (ELISA).

• Table 51: Percentage of correct results in the detection of biotechnology-derived

events in corn using Enzyme-Linked Immunosorbent Assay (ELISA).

• Table 52: Results for soybeans fortified with CP4EPSPS and A2704-12 using 

Enzyme-Linked Immunosorbent Assay (ELISA). 

• Table 53: Percentage of correct results in qualitative reports for CP4EPSPS and 

A2704-12 using Enzyme-Linked Immunosorbent Assay (ELISA). 

Quantitative Data Summaries: This section summarizes DNA based quantitative 

sample analysis data: z-scores were purposefully left blank on non-fortified (0.0%) 

samples since a z-score assumes a normal distribution and its interpretation would be 

distorted. 

• Table 54: Quantitative results and z-scores for corn fortified with T25 for all 

participants (DNA-based assays).

• Table 55: Quantitative results and z-scores for corn fortified with MON810 for all 

participants (DNA-based assays).

• Table 56: Quantitative results and z-scores for corn fortified with GA21 for all 

participants (DNA-based assays).

• Table 57: Quantitative results and z-scores for corn fortified with NK603 for all 

participants (DNA-based assays).

• Table 58: Quantitative results and z-scores for corn fortified with Herculex® for all 

participants (DNA-based assays).

• Table 59: Quantitative results and z-scores for corn fortified with MON863 for all 

participants (DNA-based assays).

• Table 60: Quantitative results and z-scores for corn fortified with Herculex® RW for 

all participants (DNA-based assays).
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• Table 61: Quantitative results and z-scores for corn fortified with MIR604 for all 

participants (DNA-based assays).

• Table 62: Quantitative results and z-scores for corn fortified with Event 3272 for all 

participants (DNA-based assays).

• Table 63: Quantitative results and z-scores for corn fortified with MON 88017 for all 

participants (DNA-based assays).

• Table 64: Quantitative results and z-scores for corn fortified with MON 89034 for all 

participants (DNA-based assays).

• Table 65: Quantitative results and z-scores for corn fortified with MIR162 for all 

participants (DNA-based assays).

• Table 66: Quantitative results and z-scores for soybeans fortified with CP4 EPSPS 

in Roundup ReadyTM for all participants (DNA-based assays).

• Table 67: Quantitative results and z-scores for soybeans fortified with A2704-12 for 

all participants (DNA-based assays).

• Table 68: Quantitative results and z-scores for soybeans fortified with CP4 EPSPS 

in Roundup ReadyTM II for all participants (DNA-based assays).

• Table 69: Quantitative results and z-scores for soybeans fortified with DP305423 

for all participants (DNA-based assays).

• Table 70: Quantitative results for 35S, NOS, and FMV in corn (DNA-based assays).

• Table 71: Quantitative results for 35S, NOS, and FMV in soybeans (DNA-based 

assays).

• Table 72: Quantitative results for corn fortified with biotechnology-derived events 

using Protein Based Lateral Flow Strip (LFS) tests.

• Table 73: Quantitative results for soybeans fortified with CP4 EPSPS as Roundup 

ReadyTM (RUR) and A2704-12 (Liberty Link) using Protein Based 

Lateral Flow Strip (LFS) tests

• Table 74: Quantitative results for soybeans fortified with CP4 EPSPS as Roundup 

ReadyTM (RUR) using Protein Based Enzyme-Linked Immunosorbent 

Assay (ELISA)

• Table 75: Descriptive statistics for participants reported quantifications relative to 

USDA/AMS/FGIS fortification levels using DNA-based assays.

• Appendix I: List of organizations who wished to be identified as a participant in the 

USDA/AMS/FGIS October 2017 Proficiency Program.
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Table 1: Qualitative results of corn fortified with 35S for all participants (DNA-based 

assays) (N = negative; P = positive; NR = not reported; Incorrect results are shown 

in boldface)

Participant Number Sample 1 Sample 2 Sample 3 Sample 4

1752 P P P N

1754 P P P N

1761 P P P N

1764 P P P N

1770 P P P N

1788 P P P N

1844 P P P N

1847 P P P N

1854 P N P P

1859 P P P N

1862 P P P N

1870 P P P N

1892 P P P N

1895 P P P N

1897 P P P N

2005 P P P N

2031 P P P N

2034 P P P N

2057 P P P N

2076 P P P N

2112 P P P N

2113 P P P P

2126 P P P N

2131 P P P N

2560 P P P N

2691 P P P N

2716 P P P N

2717 P P P N

2723 P P P N

2727 P P P N

2815 P N N N

3885 P P P N

3888 P P P N

3928 P P P N

3945 P P P N

3946 P P P N

3949 P P P N

4504 P P P N

4932 P P P N

4933 P P P N
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Participant Number Sample 1 Sample 2 Sample 3 Sample 4

4935 P P P N

4937 P P P N

4939 P P P N

4942 P P P N

4945 P P P N

4950 P P P N

4952 P P P N

4956 P P P N

4957 P P P N

4960 P P P N

4966 P P P N

4976 P P P N

4977 P P P N

4978 P P P N

4981 P P P N

4982 P P P P

4987 P P P N

7003 P P P N

7006 P P P N

7008 P P P N

7016 P P P N

7017 P P P N

7019 P P P N

7024 P P P P

7025 P P P N

7026 P P P N

7028 P P P N

7030 P P P N

7032 P P P N

7033 P N P P

7035 P P P N

7036 P P P NR

7037 P P P N

7038 P P P N

7039 P P P N

7040 P P P N

7041 P P N P

Data Sample 1 Sample 2 Sample 3 Sample 4

N, Results 77 77 77 76

# Negative 0 3 2 70

# Positive 77 74 75 6

% Correct 100.0% 96.1% 97.4% 92.1%

% Incorrect 0.0% 3.9% 2.6% 7.9%
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Table 2: Percentages of correct results, false negatives, and false positives in 

qualitative reports of 35S for all participants

Total # Reported Results 307

# Incorrect 11

% Correct 96.4%

# Provided Positives 231

# False Negative 5

% False Negative 2.2%

# Provided Negatives 76

# False Positive 6

% False Positive 7.9%

Table 3: Qualitative results of corn fortified with NOS for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1 Sample 2 Sample 3 Sample 4

1752 P P P N

1754 P P P N

1761 P P P N

1764 P P P N

1770 P P P N

1788 P P P N

1844 P P P N

1847 P P P N

1854 P P P P

1859 P P P N

1862 P P P N

1870 P P P N

1892 P P P N

1895 P P P N

1897 P P P N

2005 P P P N

2031 P P P N

2034 P P P N

2057 P P P N

2076 P P P N

2112 P P P N

2113 P P P P

2126 P P P N

2131 P P P N

2560 P P P N

2691 P P P N
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Participant Number Sample 1 Sample 2 Sample 3 Sample 4

2716 P P P N

2717 P P P N

2723 P P P N

2727 P P P N

2815 P P P N

3885 P P P N

3888 P P P N

3928 P P P N

3945 P P P N

3946 P P P N

3949 P P P N

4504 P P P N

4932 P P P N

4933 P P P N

4935 P P P N

4936 P P P N

4937 P P P N

4939 P P P N

4942 P P P N

4945 P P P N

4950 P P P P

4952 P P P N

4956 P P P N

4957 P P P N

4960 P P P N

4966 P P P N

4976 P P P N

4977 P P P N

4978 P P P N

4981 P P P N

4982 P P P N

4987 P P P N

7003 P P P N

7006 P P P N

7008 P P P N

7016 P P P N

7017 P P P N

7019 P P P N

7024 P P P P

7025 P P P N

7026 P P P N

7028 P P P N

7030 P P P N

7032 P P P N
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Participant Number Sample 1 Sample 2 Sample 3 Sample 4

7033 P N P P

7035 P P P N

7036 P P P NR

7037 P P P N

7038 P P P N

7039 P P P N

7040 P P P N

7041 P P N P

Data Sample 1 Sample 2 Sample 3 Sample 4

N, Results 78 78 78 77

# Negative 0 1 1 71

# Positive 78 77 77 6

% Correct 100.0% 98.7% 98.7% 92.2%

% Incorrect 0.0% 1.3% 1.3% 7.8%

Table 4: Percentages of correct results, false negatives, and false positives in 

qualitative reports of NOS for all participants

Total # Reported Results 311

# Incorrect 8

% Correct 97.4%

# Provided Positives 234

# False Negative 2

% False Negative 0.9%

# Provided Negatives 77

# False Positive 6

% False Positive 7.8%
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Table 5: Qualitative results of corn fortified with FMV for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1 Sample 2 Sample 3 Sample 4

1754 P P N N

1764 P N N N

1785 P N N N

1859 P N N N

1870 P N N N

1892 P N N N

1895 P N N N

2005 P N N N

2031 P N N N

2057 P N N N

2112 P N N N

2113 P N N N

2131 P N N N

2691 P N N N

2716 P N N N

2717 P N N N

2723 P N N N

3888 P N N N

3928 P N N N

3945 P N N N

3949 N N N N

4504 P N N N

4932 P N N N

4935 P N N N

4950 P N N N

4952 P N P N

4957 P N N N

4960 P N N N

4964 P N N N

4966 P N N N

4974 P N N N

4976 P N N N

4978 P N N N

4987 P N N N

7017 P N N N

7019 P N N N

7025 P N N N

7026 P N N N

7030 P P N N

7032 P N N N
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Participant Number Sample 1 Sample 2 Sample 3 Sample 4

7033 P N N N

7036 P N N NR

7037 P N N N

7038 P P P N

7039 P N N N

7040 N N N N

7041 N N N N

Data Sample 1 Sample 2 Sample 3 Sample 4

N, Results 47 47 47 46

# Negative 3 44 45 46

# Positive 44 3 2 0

% Correct 93.6% 93.6% 95.7% 100.0%

% Incorrect 6.4% 6.4% 4.3% 0.0%

Table 6: Percentages of correct results, false negatives, and false positives in 

qualitative reports of FMV for all participants

Total # Reported Results 187

# Incorrect 8

% Correct 95.7%

# Provided Positives 47

# False Negative 3

% False Negative 6.4%

# Provided Negatives 140

# False Positive 5

% False Positive 3.6%
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Table 7: Qualitative results of corn fortified with T25 for all participants (DNA-based 

assays) (N = negative; P = positive; NR = not reported; Incorrect results are shown 

in boldface)

Participant Number Sample 1

0.0%

Sample 2

0.0%

Sample 3

0.8%

Sample 4

0.0%

1752 N N P N

1764 N N P N

1785 N N P N

1844 N N P N

1854 P P P P

1859 N N P N

1862 N N P N

1892 P P P N

1895 N N P N

1897 N N P N

2005 P P P N

2034 N N P N

2060 P P P N

2089 N N P N

2112 N N P N

2113 N N N N

2126 N N P N

2131 N N P N

2560 N N P N

2569 N N P N

2691 P P N N

2694 N N P N

2732 N N P N

3928 N N P N

3929 N N P N

4504 P P P N

4936 N N P N

4937 N N P N

4942 N N P N

4945 N N P N

4950 P P P N

4957 N N P N

4964 P P P N

4966 N N P N

4976 N N P N

7025 N N P N

7027 N N P N
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Participant Number Sample 1

0.0%

Sample 2

0.0%

Sample 3

0.8%

Sample 4

0.0%

7032 N N P N

7036 N N P N

7037 N N P N

7039 N N P N

Data Sample 1

0.0%

Sample 2

0.0%

Sample 3

0.8%

Sample 4

0.0%

N, Results 41 41 41 41

# Negative 33 33 2 40

# Positive 8 8 39 1

% Correct 80.5% 80.5% 95.1% 97.6%

% Incorrect 19.5% 19.5% 4.9% 2.4%

Table 8: Percentages of correct results, false negatives, and false positives in 

qualitative reports of T25 for all participants

Total # Reported Results 164

# Incorrect 19

% Correct 88.4%

# Provided Positives 41

# False Negative 2

% False Negative 4.9%

# Provided Negatives 123

# False Positive 17

% False Positive 13.8%

Table 9: Qualitative results of corn fortified with MON810 for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1

0.5%

Sample 2

2.0%

Sample 3

0.0%

Sample 4

0.0%

1761 P P N N

1785 P P N N

1788 P P N N

1844 P P N N

1854 P P N N

1859 P P N N

1862 P P N N

1892 P P N N
18

Participant Number Sample 1

0.5%

Sample 2

2.0%

Sample 3

0.0%

Sample 4

0.0%

1895 P P N N

1897 P P N N

2034 P P N N

2060 P P N N

2089 P P N N

2113 P P N N

2126 P P N N

2131 P P N N

2560 P P N N

2569 P P N N

2691 N N P N

4937 P P N N

4945 P P N N

4950 N P N N

4964 P P N N

4966 P P N N

4978 P P N N

4987 P P N N

7025 P P N N

7027 P P N N

7032 P P N N

7036 P P N N

7037 P P N N

7039 P P N N

Data Sample 1

0.5%

Sample 2

2.0%

Sample 3

0.0%

Sample 4

0.0%

N, Results 32 32 32 32

# Negative 2 1 31 32

# Positive 30 31 1 0

% Correct 93.8% 96.9% 96.9% 100.0%

% Incorrect 6.3% 3.1% 3.1% 0.0%
19

Table 10: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON810 for all participants

Total # Reported Results 128

# Incorrect 4

% Correct 96.9%

# Provided Positives 64

# False Negative 3

% False Negative 4.7%

# Provided Negatives 64

# False Positive 1

% False Positive 1.6%

Table 11: Qualitative results of corn fortified with GA21 for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1

0.0%

Sample 2

0.2%

Sample 3

0.5%

Sample 4

0.0%

1785 N P P N

1788 N P P N

1844 N P P N

1854 P P P P

1859 N P P N

1862 N N P N

1892 N P P N

1895 N P P N

1897 N P P N

2034 N P P N

2060 N P P N

2089 N P P N

2112 N P P N

2113 N P P N

2126 N P P N

2131 N P P N

2560 N P P N

2569 N P P N

2691 N P P N

2717 N P P N

3929 N P P N

4937 N P P N

4942 N P P N

4945 N P P N
20

Participant Number Sample 1

0.0%

Sample 2

0.2%

Sample 3

0.5%

Sample 4

0.0%

4950 N P P N

4960 N P P N

4964 N P P N

4966 N P P N

4978 N P P N

7025 N P P N

7027 N P P N

7032 P P N N

7036 N P P N

7037 N P P N

7039 N P P N

Data Sample 1

0.0%

Sample 2

0.2%

Sample 3

0.5%

Sample 4

0.0%

N, Results 35 35 35 35

# Negative 33 1 1 34

# Positive 2 34 34 1

% Correct 94.3% 97.1% 97.1% 97.1%

% Incorrect 5.7% 2.9% 2.9% 2.9%

Table 12: Percentages of correct results, false negatives, and false positives in 

qualitative reports of GA21 for all participants

Total # Reported Results 140

# Incorrect 5

% Correct 96.4%

# Provided Positives 70

# False Negative 2

% False Negative 2.9%

# Provided Negatives 70

# False Positive 3

% False Positive 4.3%

Table 13: Qualitative results of corn fortified with NK603 for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1

0.0%

Sample 2

0.0%

Sample 3

1.5%

Sample 4

0.0%

1752 N N P N

1761 N N P N
21

Participant Number Sample 1

0.0%

Sample 2

0.0%

Sample 3

1.5%

Sample 4

0.0%

1785 N N P N

1788 N N P N

1844 N N P N

1854 P P P P

1859 N N P N

1862 N N P N

1892 N N P N

1895 N N P N

1897 N N P N

2034 N N P N

2060 N N P N

2089 N N P N

2113 N N P N

2126 N N P N

2131 N N P N

2560 N N P N

2569 N N P N

2691 N N P N

2720 N N P N

2824 N N P N

3928 N N P N

3929 N N P N

4937 N N P N

4942 N N P N

4945 N N P N

4950 N N P N

4956 N N P N

4960 N N P N

4964 N N P N

4966 N N P N

4974 N N P N

4978 N N P N

4987 N N P N

7025 N N P N

7027 N N P N

7032 N N P N

7036 N N P N

7037 N N P N

7039 N N P N
22

Data Sample 1

0.0%

Sample 2

0.0%

Sample 3

1.5%

Sample 4

0.0%

N, Results 41 41 41 41

# Negative 40 40 0 40

# Positive 1 1 41 1

% Correct 97.6% 97.6% 100.0% 97.6%

% Incorrect 2.4% 2.4% 0.0% 2.4%

Table 14: Percentages of correct results, false negatives, and false positives in 

qualitative reports of NK603 for all participants

Total # Reported Results 164

# Incorrect 3

% Correct 98.2%

# Provided Positives 41

# False Negative 0

% False Negative 0.0%

# Provided Negatives 123

# False Positive 2

% False Positive 1.6%

Table 15: Qualitative results of corn fortified with Herculex® for all participants 

(DNA-based assays) (N = negative; P = positive; NR = not reported; Incorrect 

results are shown in boldface)

Participant Number Sample 1

0.0%

Sample 2

1.0%

Sample 3

0.2%

Sample 4

0.0%

1752 N P P N

1761 N P P N

1785 N P P N

1844 N P P N

1844 N P P N

1854 N P P P

1859 N P P N

1862 N P P N

1892 N P P N

1895 N P P N

1897 N P P N

2034 N P P N

2060 N P P N

2089 N P P N

2112 N P P N

2113 N P P N
23

Participant Number Sample 1

0.0%

Sample 2

1.0%

Sample 3

0.2%

Sample 4

0.0%

2126 N P P N

2131 N P N N

2560 N P P N

2569 N P P N

2691 N P P N

2824 N P P N

3929 N P P N

4937 N P P N

4942 N P P N

4950 N P P N

4960 N P P N

4964 N P P N

4966 N P P N

4978 N P P N

7025 N P P N

7027 N P P N

7032 N P P N

7037 N P P N

7039 N P P N

Data Sample 1

0.0%

Sample 2

1.0%

Sample 3

0.2%

Sample 4

0.0%

N, Results 35 35 35 35

# Negative 35 0 1 34

# Positive 0 35 34 1

% Correct 100.0% 100.0% 97.1% 97.1%

% Incorrect 0.0% -2.9% 2.9% 2.9%

Table 16: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Herculex® for all participants

Total # Reported Results 140

# Incorrect 1

% Correct 99.3%

# Provided Positives 70

# False Negative 0

% False Negative 0.0%

# Provided Negatives 70

# False Positive 1

% False Positive 1.4%
24

Table 17: Qualitative results of corn fortified with MON863 for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1

1.0%

Sample 2

0.0%

Sample 3

0.2%

Sample 4

0.0%

1785 P N P N

1788 P N P N

1844 P N P N

1854 P P P P

1859 P N P N

1862 P N P N

1892 P N P N

1895 P N N N

1897 P N P N

2034 P N P N

2060 P N P N

2089 P N P N

2112 P N P N

2113 P P P P

2126 P N P N

2131 P N P N

2560 P N P N

2569 P N P N

2691 P N P N

3929 P N P N

4937 P N P N

4942 P N P N

4945 P N P N

4950 P N P N

4956 P N P N

4964 P N P N

4966 P N P N

4974 P N P N

7025 P N P N

7027 P N P N

7032 P NR P N

7036 P P P NR

7037 P N P N

7039 P N P N
25

Data Sample 1

1.0%

Sample 2

0.0%

Sample 3

0.2%

Sample 4

0.0%

N, Results 34 33 34 33

# Negative 0 30 1 31

# Positive 34 3 33 2

% Correct 100.0% 90.9% 100.0% 93.9%

% Incorrect 0.0% 9.1% 0.0% 6.1%

Table 18: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON863 for all participants

Total # Reported Results 134

# Incorrect 5

% Correct 96.3%

# Provided Positives 68

# False Negative 1

% False Negative 1.5%

# Provided Negatives 66

# False Positive 5

% False Positive 7.6%

Table 19: Qualitative results of corn fortified with Herculex® RW for all participants 

(DNA-based assays) (N = negative; P = positive; NR = not reported; Incorrect 

results are shown in bold face)

Participant Number Sample 1

0.5%

Sample 2

0.0%

Sample 3

1.0%

Sample 4

0.0%

1752 P N P N

1761 P N P N

1785 P N P N

1844 P N P N

1859 P N P N

1862 P N P N

1892 P N P N

1895 P N P N

2005 P N P N

2034 P N P N

2060 P N P N

2089 P N P N

2113 P N P N

2126 P N P N

2131 P N P N

2560 P N P N
26

Participant Number Sample 1

0.5%

Sample 2

0.0%

Sample 3

1.0%

Sample 4

0.0%

2569 P N P N

2691 P N P N

3929 P N P N

4504 P N P N

4937 P N P N

4942 P N P N

4945 P N P N

4950 N N P N

4952 P N P N

4960 P N P N

4964 P N P N

4966 P N P N

4974 P N P N

4978 P N P N

7025 P N P N

7027 P N P N

7037 P N P N

Data Sample 1

0.5%

Sample 2

0.0%

Sample 3

1.0%

Sample 4

0.0%

N, Results 33 33 33 33

# Negative 1 33 0 33

# Positive 32 0 33 0

% Correct 97.0% 100.0% 100.0% 100.0%

% Incorrect 3.0% 0.0% 0.0% 0.0%
27

Table 20: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Herculex® RW for all participants

Total # Reported Results 132

# Incorrect 1

% Correct 99.2%

# Provided Positives 66

# False Negative 1

% False Negative 1.5%

# Provided Negatives 66

# False Positive 0

% False Positive 0.0%

Table 21: Qualitative results of corn fortified with MIR604 for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1

0.0%

Sample 2

0.0%

Sample 3

0.5%

Sample 4

0.0%

1785 N N P N

1844 N N P N

1854 P P P P

1859 N N P N

1862 N N P N

1895 N N P N

1897 N N P N

2034 N N P N

2060 N N P N

2089 N N P N

2113 N N P N

2126 N N P N

2131 N N P N

2560 N N P N

2569 N N P N

2691 N N P N

2717 N N P N

2720 N N P N

2824 N N P N

3928 N N P N

3929 N N P N

4937 N N P N

4942 N N P N

4945 N N P N

4950 N N P P
28

Participant Number Sample 1

0.0%

Sample 2

0.0%

Sample 3

0.5%

Sample 4

0.0%

4956 N N P N

4960 N N P N

4964 N N P N

4966 N N P N

4978 N N P N

7025 N N P N

7027 N N P N

7032 N N P N

7036 N N P N

7037 N N P N

7039 N N P N

Data Sample 1

0.0%

Sample 2

0.0%

Sample 3

0.5%

Sample 4

0.0%

N, Results 36 36 36 36

# Negative 35 35 0 34

# Positive 1 1 36 2

% Correct 97.2% 97.2% 100.0% 94.4%

% Incorrect 2.8% 2.8% 0.0% 5.6%

Table 22: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MIR604 for all participants

Total # Reported Results 144

# Incorrect 4

% Correct 97.2%

# Provided Positives 36

# False Negative 0

% False Negative 0.0%

# Provided Negatives 108

# False Positive 4

% False Positive 3.7%
29

Table 23: Qualitative results of corn fortified with Event 3272 for all participants 

(DNA-based assays) (N = negative; P = positive; NR = not reported; Incorrect 

results are shown in boldface)

Participant Number Sample 1

1.0%

Sample 2

0.0%

Sample 3

0.0%

Sample 4

0.0%

1761 P N N N

1785 P N N N

1844 P N N N

1859 P N N N

1895 P N N N

2005 P N N N

2034 P N N N

2113 P N N N

2126 P N N N

2131 P N N N

2691 P N N N

3928 P N N N

4504 P N N N

4937 P N N N

4942 P N N N

4945 P N N N

4950 P N N N

4957 P N N N

4960 P N N N

4964 P N N N

4966 P N N N

4974 P N N N

4978 P N N N

4987 P N N N

7025 P N N N

7027 P N N N

7036 P N N N

7037 P N N N

Data Sample 1

1.0%

Sample 2

0.0%

Sample 3

0.0%

Sample 4

0.0%

N, Results 28 28 28 28

# Negative 0 28 28 28

# Positive 28 0 0 0

% Correct 100.0% 100.0% 100.0% 100.0%

% Incorrect 0.0% 0.0% 0.0% 0.0%
30

Table 24: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Event 3272 for all participants

Total # Reported Results 112

# Incorrect 0

% Correct 100.0%

# Provided Positives 28

# False Negative 0

% False Negative 0.0%

# Provided Negatives 84

# False Positive 0

% False Positive 0.0%

Table 25: Qualitative results of Corn fortified with MON88017 for all participants 

(DNA-based assays) (N = negative; P = positive; NR = not reported; Incorrect 

results are shown in boldface)

Participant Number Sample 1

0.2%

Sample 2

0.5%

Sample 3

0.0%

Sample 4

0.0%

1761 P P N N

1785 P P N N

1844 P P N N

1854 P P P P

1859 P P N N

1862 P P N N

1895 P P N N

1897 P P N N

2031 P P N N

2034 P P N N

2060 P P N N

2089 P P N N

2113 P P N N

2126 P P N N

2131 N N N N

2560 P P N N

2569 P P N N

2691 P P N N

2824 P P N N

3929 P P N N

4504 P P N N

4937 P P N N

4942 P P N N

4945 P P N N
31

Participant Number Sample 1

0.2%

Sample 2

0.5%

Sample 3

0.0%

Sample 4

0.0%

4950 N P N N

4960 P P N N

4964 P P N N

4966 P P N N

4974 P P N N

4978 P P N N

4987 P P N N

7025 P P N N

7027 P P N N

7032 P P N N

7036 P P N N

7037 P P N N

7039 P P N N

Data Sample 1

0.2%

Sample 2

0.5%

Sample 3

0.0%

Sample 4

0.0%

N, Results 37 37 37 37

# Negative 2 1 36 36

# Positive 35 36 1 1

% Correct 94.6% 100.0% 97.3% 97.3%

% Incorrect 5.4% 0.0% 2.7% 2.7%

Table 26: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON88017 for all participants

Total # Reported Results 148

# Incorrect 5

% Correct 96.6%

# Provided Positives 74

# False Negative 3

% False Negative 4.1%

# Provided Negatives 74

# False Positive 2

% False Positive 2.7%
32

Table 27: Qualitative results of corn fortified with MON89034 for all participants 

(DNA-based assays) (N = negative; P = positive; NR = not reported; Incorrect 

results are shown in bold face)

Participant Number Sample 1

0.5%

Sample 2

0.0%

Sample 3

0.0%

Sample 4

0.0%

1761 P N N N

1785 P N N N

1844 P N N N

1854 P P P P

1859 P N N N

1862 P N N N

1895 P N N N

1897 P N N N

2031 P N N N

2034 P N N N

2060 P N N N

2089 P N N N

2113 P N N N

2126 P N N N

2131 P N N N

2560 P N N N

2569 P N N N

2691 P N N N

2824 P N N N

3929 P N N N

4504 P N N N

4937 P N N N

4942 P N N N

4945 P N N N

4950 P N N N

4957 P N N N

4960 P N N N

4964 P N N N

4966 P N N N

4976 P N N N

4978 P N N N

7025 P N N N

7027 P N N N

7032 P N N N

7036 P N N N

7037 P N N N

7039 P N N N
33

Participant Number Sample 1

0.5%

Sample 2

0.0%

Sample 3

0.0%

Sample 4

0.0%

7040 P P P N

7041 P P N P

Data Sample 1

0.5%

Sample 2

0.0%

Sample 3

0.0%

Sample 4

0.0%

N, Results 39 39 39 39

# Negative 0 36 37 37

# Positive 39 3 2 2

% Correct 102.6% 92.3% 94.9% 94.9%

% Incorrect -2.6% 7.7% 5.1% 5.1%

Table 28: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MON89034 for all participants

Total # Reported Results 156

# Incorrect 7

% Correct 95.5%

# Provided Positives 39

# False Negative 0

% False Negative 0.0%

# Provided Negatives 117

# False Positive 7

% False Positive 6.0%

Table 29: Qualitative results of corn fortified with MIR162 for all participants (DNAbased assays) (N = negative; P = positive; NR = not reported; Incorrect results are 

shown in boldface)

Participant Number Sample 1

0.0%

Sample 2

1.0%

Sample 3

0.0%

Sample 4

0.0%

1785 N P N N

1844 N P N N

1854 P P P P

1859 N P N N

1862 N P N N

1895 N P N N

1897 N P N N

2034 N P N N

2060 N P N N

2089 N P N N

2113 N P N N
34

Participant Number Sample 1

0.0%

Sample 2

1.0%

Sample 3

0.0%

Sample 4

0.0%

2126 N P N N

2131 N P N N

MIR 162 Sample 1 Sample 2 Sample 3 Sample 4

Participant Number 0.0% 1.0% 0.0% 0.0%

2560 N P N N

2569 N P N N

2824 N P N N

3928 N P N N

3929 N P N N

4504 N P N N

4937 N P N N

4942 N P N N

4950 N P P N

4960 N P N N

4964 N P N N

4966 N P N N

4987 N P N N

7025 N P N N

7027 N P N N

7032 N P N N

7036 N P N N

7037 N P N N

7039 N P N N

Data Sample 1

0.0%

Sample 2

1.0%

Sample 3

0.0%

Sample 4

0.0%

N, Results 32 32 32 32

# Negative 31 0 30 31

# Positive 1 32 2 1

% Correct 96.9% 100.0% 93.8% 96.9%

% Incorrect 3.1% 0.0% 6.3% 3.1%
35

Table 30: Percentages of correct results, false negatives, and false positives in 

qualitative reports of MIR162 for all participants

Total # Reported Results 128

# Incorrect 4

% Correct 96.9%

# Provided Positives 32

# False Negative 0

% False Negative 0.0%

# Provided Negatives 96

# False Positive 4

% False Positive 4.2%

Table 31: Qualitative results of soybeans fortified with CP4 EPSPS (Roundup 

ReadyTM) for all participants (DNA-based assays) (N = negative; P = positive; 

Incorrect results are shown in boldface)

Participant Number Sample 1

0.0%

Sample 2

0.5%

1752 N P

1844 N P

1847 N P

1854 P P

1859 N P

1862 N P

1892 N P

1897 N P

2034 N P

2089 N P

2131 N P

2560 N P

2569 N P

2717 N P

3928 N P

4937 N P

4945 N P

4950 N P

4956 N P

4964 N P

4966 N P

4974 N P

4987 N P

7027 N P

7032 N P
36

Participant Number Sample 1

0.0%

Sample 2

0.5%

7036 N P

7037 N P

7039 N P

Data Sample 1

0.0%

Sample 2

0.5%

N, Results 28 28

# Negative 27 0

# Positive 1 28

% Correct 96.4% 100.0%

% Incorrect 3.6% 0.0%

Table 32: Percentages of correct results, false negatives, and false positives in 

qualitative reports of CP4 EPSPS (Roundup ReadyTM) for all participants

Total # Reported Results 56

# Incorrect 1

% Correct 98.2%

# Provided Positives 28

# False Negative 0

% False Negative 0.0%

# Provided Negatives 28

# False Positive 1

% False Positive 3.6%

Table 33: Qualitative results of soybeans fortified with A2704-12 (Liberty Link®) for 

all participants (DNA-based assays) (N = negative; P = positive; Incorrect results 

are shown in boldface)

Participant Number Sample 1

0.0%

Sample 2

0.5%

1752 N P

1785 N P

1844 N P

1847 N P

1859 N P

1862 N P

1892 N P

1897 N P

2034 N P

2060 N P
37

Participant Number Sample 1

0.0%

Sample 2

0.5%

2089 N P

2131 N P

2560 N P

2691 N P

2692 N P

3928 N P

4936 N P

4937 N P

4942 N P

4945 N P

4950 N P

4957 P N

4964 N P

4966 N P

7027 N P

7032 N P

7034 N P

7036 N P

7037 N P

Data Sample 1

0.0%

Sample 2

0.5%

N, Results 29 29

# Negative 28 1

# Positive 1 28

% Correct 96.6% 96.6%

% Incorrect 3.4% 3.4%

Table 34: Percentages of correct results, false negatives, and false positives in 

qualitative reports of A2704-12 (Liberty Link®) for all participants

Total # Reported Results 58

# Incorrect 2

% Correct 96.6%

# Provided Positives 29

# False Negative 1

% False Negative 3.4%

# Provided Negatives 29

# False Positive 1

% False Positive 3.4%
38

Table 35: Qualitative results of soybeans fortified with CP4 EPSPS (Roundup 

ReadyTM II) for all participants (DNA-based assays) (N = negative; P = positive; 

Incorrect results are shown in boldface).

Participant Number Sample 1

0.0%

Sample 2

0.0%

1752 N N

1764 N N

1785 N N

1844 N N

1847 N N

1859 N N

1862 N N

1870 N N

1892 N N

1897 N N

2005 N N

2034 N N

2060 N N

2089 N N

2112 N N

2131 N N

2560 N N

2569 N N

2691 N N

2692 N N

2727 N N

2824 N N

4504 N N

4932 N N

4935 N N

4936 N N

4937 N N

4942 N P

4945 N N

4950 N N

4957 N N

4960 N N

4964 N N

4966 N N

4976 N N

4978 N N

4987 N N
39

Participant Number Sample 1

0.0%

Sample 2

0.0%

7025 N N

7027 N N

7032 N N

7037 N N

Data Sample 1

0.0%

Sample 2

0.0%

N, Results 41 41

# Negative 41 40

# Positive 0 1

% Correct 100.0% 97.6%

% Incorrect 0.0% 2.4%

Table 36: Percentages of correct results, false negatives, and false positives in 

qualitative reports of CP4 EPSPS (Roundup ReadyTM II) for all participants

Total # Reported Results 82

# Incorrect 1

% Correct 98.8%

# Provided Positives 0

# False Negative 0

% False Negative 0.0%

# Provided Negatives 41

# False Positive 1

% False Positive 2.4%

Table 37: Qualitative results of soybeans fortified with DP305423 for all participants 

(DNA-based assays) (N = negative; P = positive; Incorrect results are shown in bold 

face)

Participant Number Sample 1

0.0%

Sample 2

0.5%

1752 N P

1761 N P

1785 N P

1844 N P

1847 N P

1854 P P

1859 N P

1862 N P

1897 N P
40

Participant Number Sample 1

0.0%

Sample 2

0.5%

2005 N P

2034 N P

2060 N P

2131 N P

2691 N P

2692 N P

2717 N P

2824 N P

3929 N P

4937 N P

4942 N P

4945 N P

4950 P N

4956 N P

4957 P N

4960 N P

4964 N P

4966 N P

4974 N P

4978 N P

4987 N P

7025 N P

7027 N P

7034 N P

7036 N P

7037 N P

7038 N P

7039 N P

7040 N P

7041 N P

Data Sample 1

0.0%

Sample 2

0.5%

N, Results 39 39

# Negative 36 2

# Positive 3 37

% Correct 92.3% 94.9%

% Incorrect 7.7% 5.1%
41

Table 38: Percentages of correct results, false negatives, and false positives in 

qualitative reports of DP305423 for all participants

Total # Reported Results 78

# Incorrect 5

% Correct 93.6%

# Provided Positives 39

# False Negative 2

% False Negative 5.1%

# Provided Negatives 39

# False Positive 3

% False Positive 7.7%

Table 39: Qualitative results for soybeans fortified with 35S for all participants 

(DNA-based assays) (N = negative; P = positive; Incorrect results are shown in bold 

face) 

Participant Number Sample 1

N

Sample 2

P

1752 N P

1754 N P

1761 N P

1764 N P

1770 N P

1785 N P

1844 N P

1854 P P

1859 N P

1870 N P

1892 N P

1895 N P

1897 N P

2005 N P

2031 N P

2034 N P

2057 N P

2076 N P

2112 N P

2131 N P

2560 N P

2691 N P

2692 N P

2716 N P

2717 N P

2723 N P
42

Participant Number Sample 1

N

Sample 2

P

2727 N P

2815 P P

3885 P N

3888 N P

3928 N P

3929 N P

3945 N P

3946 N P

3949 P P

4504 N P

4932 N P

4933 N P

4935 N P

4937 N P

4939 N P

4942 N P

4945 N P

4950 P P

4952 N P

4956 N P

4960 N P

4966 N P

4974 N P

4976 N P

4977 N P

4978 N P

4981 N P

4987 N P

7003 N P

7006 N P

7008 N P

7016 N P

7017 N P

7019 N P

7024 P P

7025 N P

7028 N P

7030 N P

7032 N P

7033 P N

7035 N P

7036 N P

7037 N P
43

Participant Number Sample 1

N

Sample 2

P

7039 N P

Data Sample 1

N

Sample 2

P

N, Results 70 70

# Negative 63 2

# Positive 7 68

% Correct 90.0% 97.1%

% Incorrect 10.0% 2.9%

Table 40: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Soy 35S for all participants

Total # Reported Results 140

# Incorrect 9

% Correct 93.6%

# Provided Positives 70

# False Negative 2

% False Negative 2.9%

# Provided Negatives 70

# False Positive 7

% False Positive 10.0%

Table 41: Qualitative results of soybeans fortified with NOS for all participants 

(DNA-based assays) (N = negative; P = positive; Incorrect results are shown in bold 

face)

Participant Number Sample 1

N

Sample 2

P

1752 N P

1754 N P

1761 N P

1764 N P

1770 N P

1785 N P

1788 N P

1854 N P

1859 N P

1862 N P

1870 N P

1892 N P
44

Participant Number Sample 1

N

Sample 2

P

1895 N P

1897 N P

2005 N P

2031 N P

2034 N P

2057 N P

2076 N P

2112 N P

2131 N P

2560 N P

2691 N P

2692 N P

2716 N P

2717 N P

2723 N P

2727 N P

2815 P P

3885 P N

3887 N P

3888 N P

3928 N P

3929 N P

3945 N P

3946 N P

3949 N P

4504 N P

4932 N P

4933 N P

4935 N P

4936 N P

4937 N P

4939 N P

4942 N P

4945 N P

4950 P P

4952 N P

4956 N P

4960 N P

4966 N P

4974 N P

4976 N P

4977 N P

4978 N P
45

Participant Number Sample 1

N

Sample 2

P

4981 N P

4987 N P

7003 N P

7006 N P

7008 N P

7016 N P

7017 N P

7019 N P

7024 P P

7025 N P

7028 N P

7030 N P

7032 N P

7033 P N

7035 N P

7036 N P

7037 N P

7039 N P

Data Sample 1

N

Sample 2

P

N, Results 73 73

# Negative 68 2

# Positive 5 71

% Correct 93.2% 97.3%

% Incorrect 6.8% 2.7%

Note: The NOS trait serves as a terminator in RUR and DP305423 in this list of events.

Table 42: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Soy NOS for all participants

Total # Reported Results 146

# Incorrect 7

% Correct 95.2%

# Provided Positives 73

# False Negative 2

% False Negative 2.7%

# Provided Negatives 73

# False Positive 5

% False Positive 6.8%
46

Table 43: Qualitative results of soybeans fortified with FMV for all participants 

(DNA-based assays) (N = negative; P = positive; Incorrect results are shown in bold 

face)

Participant Number Sample 1

N

Sample 2

N

1754 N N

1764 N N

1785 N N

1844 N N

1859 N N

1870 N N

1892 N N

1895 N N

2005 N N

2031 N N

2034 N N

2057 N N

2112 N N

2131 N N

2691 N N

2716 N N

2717 N N

3887 N N

3888 N N

3929 N N

3945 N N

4504 N N

4932 N N

4935 N N

4936 N N

4945 N N

4950 N P

4952 N N

4960 N N

4964 N N

4966 N N

4974 N P

4976 N N

4978 N N

4987 N N

7017 N N

7019 N N

7025 N N

7030 N N
47

Participant Number Sample 1

N

Sample 2

N

7032 N N

7033 N N

7036 N N

7037 N N

7039 N N

Data Sample 1

N

Sample 2

N

N, Results 44 44

# Negative 44 42

# Positive 0 2

% Correct 100.0% 95.5%

% Incorrect 0.0% 4.5%

Note: The FMV trait is a promoter in RURII in this list of events.

Table 44: Percentages of correct results, false negatives, and false positives in 

qualitative reports of Soy FMV for all participants

Total # Reported Results 88

# Incorrect 2

% Correct 97.7%

# Provided Positives 0

# False Negative 0

% False Negative 0.0%

# Provided Negatives 88

# False Positive 2

% False Positive 2.3%
48

Table 45: Composite percentages of correct results, false negatives, and false 

positives in qualitative reports for each biotechnology-derived event for all 

participants (DNA-based assays) 

N = total number of results submitted for an event; % False Negative = [# False Negatives 

/ # Provided Positives] x 100; % False Positives = [#False Positives / # Provided 

Negatives] x100.

Event 35S NOS FMV T25 MON810

N, Results 307 311 187 164 128

Reported Incorrect 11 8 8 19 4

% Correct 96.4% 97.4% 95.7% 88.4% 96.9%

# Provided Positives 231 234 47 41 64

N, False Negatives 5 2 3 2 3

% False Negative 2.2% 0.9% 6.4% 4.9% 4.7%

# Provided Negatives 76 77 140 123 64

N, False Positives 6 6 5 17 1

% False Positives 7.9% 7.8% 3.6% 13.8% 1.6%

Event GA21 NK603 Herculex® MON863 Herculex® 

RW

N, Results 140 164 140 134 132

Reported Incorrect 5 3 1 5 1

% Correct 96.4% 98.2% 99.3% 96.3% 99.2%

# Provided Positives 70 41 70 68 66

N, False Negatives 2 0 0 1 1

% False Negative 2.9% 0.0% 0.0% 1.5% 1.5%

# Provided Negatives 70 123 70 66 66

N, False Positives 3 2 1 5 0

% False Positives 4.3% 1.6% 1.4% 7.6% 0.0%

Event MIR604 EV3272 MON88017 MON89034 MIR162 RUR

N, Results 144 112 148 156 128 56

Reported Incorrect 4 0 5 7 4 1

% Correct 97.2% 100.0% 96.6% 95.5% 96.9% 98.2%

# Provided Positives 36 28 74 39 32 28

N, False Negatives 0 0 3 0 0 0

% False Negative 0.0% 0.0% 4.1% 0.0% 0.0% 0.0%

# Provided Negatives 108 84 74 117 96 28

N, False Positives 4 0 2 7 4 1

% False Positives 3.7% 0.0% 2.7% 6.0% 4.2% 3.6%
49

Event LL RUR II DP305423 Soy 35S Soy NOS Soy FMV

N, Results 58 82 78 140 146 88

Reported Incorrect 2 1 5 9 7 2

% Correct 96.6% 98.8% 93.6% 93.6% 95.2% 97.7%

# Provided Positives 29 0 39 70 73 0

N, False Negatives 1 0 2 2 2 0

% False Negative 3.4% 0.0% 5.1% 2.9% 2.7% 0.0%

# Provided Negatives 29 41 39 70 73 88

N, False Positives 1 1 3 7 5 2

% False Positives 3.4% 2.4% 7.7% 10.0% 6.8% 2.3%

Figure 1: Group average of percentage correct for Qualitative reports on each 

event (DNA-based assays)

Embedded numbers represent the total number of reported results for that event. Data 

are shown on a composite basis (i.e., all participants results combined) extracted from 

the percentage correct scores in Table 45.
50

Table 46: Protein Based Lateral Flow Strip (LFS) Testing results for the detection 

of biotechnology-derived events in corn (N = negative; P = positive; NR = not 

reported; Incorrect results are shown in boldface)

Participant Number Sample 1 Sample 2 Sample 3 Sample 4

T 25 0.00% 0.00% 0.80% 0.00% LOD

1843 N N P N 0.5%

1895 N N N* N 0.5%

2815 N N P N Not Provided

4994 N N N N Not Provided

EPSPS 

(NK603/MON88017) 0.2% 0.50% 1.50% 0.00%

1843 P P P N 0.5%

1895 N* N* P N 0.5%

2815 P P P N Not Provided

3931 P P P P 0.1%

4902 P P P P 0.1%

4994 N P P N Not Provided

Cry1Ab (MON810) 0.50% 2.00% 0.00% 0.00%

1843 N N N N Not Provided

1895 N* N N N 0.8%

2815 P P N N Not Provided

3931 P P P P 0.5%

4902 P P P P 0.5%

4994 N N N N Not Provided

Herculex 0.0% 1.00% 0.20% 0.00%

1843 N N* N* N 0.5%

1895 N N* N* N 0.5%

2815 N P P N Not Provided

3931 N P P N 0.9%

4902 N P P N 0.9%

4994 N N N N Not Provided

Cry3Bb1 

(MON863/MON88017) 1.20% 0.50% 0.20% 0.00%

1843 P P P N 0.5%

1895 P N* N* N 0.5%

2815 P P P N Not Provided

4994 P N N N Not Provided

Hclx RW 0.50% 0.00% 1.00% 0.00%

1843 P N P N 0.5%

1895 P N P N 0.5%

2815 P N P N Not Provided

4994 P N P N Not Provided
51

MIR 604 0.00% 0.00% 0.50% 0.00%

1843 N N P N 1.0%

1895 N N P N 1.0%

2815 N N P N Not Provided

4994 N N P N Not Provided

Cry2A (MON89034) 0.50% 0.00% 0.00% 0.00%

1843 N* N N N 1.0%

Vip3A (MIR162) 0.00% 1.00% 0.00% 0.00%

1843 N P N N 0.25%

Event3272 0.00% 1.00% 0.00% 0.00%

4994 N N N N Not Provided

* - Fortification level/Consensus Mean below Participants’ LOD, assessed as Provided 

Negative

Table 47: Percentage of correct results, false negatives, and false positives in 

reports for biotechnology-derived events in corn using Protein Based Lateral Flow 

Strip (LFS) Testing 

Event T25 EPSPS Cry1Ab Herculex Cry3Bb1

Total # Reported Results 16 24 24 24 16

# Incorrect 1 3 9 2 2

% Correct 93.8% 87.5% 62.5% 91.7% 87.5%

# Provided Positives 3 16 11 12 12

# False Negatives 1 1 5 2 2

% False Negatives 33.3% 6.3% 45.5% 16.7% 16.7%

# Provided Negatives 12 6 12 12 4

# False Positives 0 2 4 0 0

% False Positive 0.0% 33.3% 33.3% 0.0% 0.0%

Event Hclx RW MIR 604 Cry2A Vip3A Event3272

Total # Reported Results 16 16 4 4 4

# Incorrect 0 0 0 0 1

% Correct 100.0% 100.0% 100.0% 100.0% 75.0%

# Provided Positives 8 4 0 1 1

# False Negatives 0 0 0 0 1

% False Negatives 0.0% 0.0% 0.0% 0.0% 100.0%

# Provided Negatives 8 12 4 3 3

# False Positives 0 0 0 0 0

% False Positive 0.0% 0.0% 0.0% 0.0% 0.0%
52

Table 48: Results for soybeans fortified with CP4EPSPS (RUR/RURII) and A2704-

12 for all participants using Protein Based Lateral Flow Strip (LFS) Testing (N = 

negative; P = positive; NR = not reported; Incorrect results are shown in boldface)

CP4 EPSPS (RUR & RURII)

Participant Number

Sample 1

0.00%

Sample 2

0.50% LOD

1782 N P 0.10%

1843 N P 0.10%

1895 N P 0.25%

2126 N P 0.10%

2815 N P Not Provided

3931 N P 0.10%

4902 N P 0.10%

4994 N P Not Provided

A2704-12 (Liberty Link)

Participant Number

Sample 1

0.0%

Sample 2

0.5% LOD

1782 N P 0.5%

1843 N P 0.5%

1895 N P 0.5%

2815 N P Not Provided

4994 N P Not Provided

Table 49: Percentage of correct results in qualitative reports for CP4EPSPS and 

A2704-12 for all participants using Protein Based Lateral Flow Strip (LFS) Testing

Event

CP4 EPSPS (RUR & RURII) A2704-12 (LL)

Total # Reported results 16 10

# Incorrect 0 0

% Correct 100.0% 100.0%

# Provided Positives 8 5

# False Negative 0 0

% False Negative 0.0% 0.0%

# Provided Negatives 8 5

# False Positive 0 0

% False Positive 0.0% 0.0%
53

Table 50: Results for the detection of biotechnology-derived events in corn using 

Protein Based Enzyme-Linked Immunosorbent Assay (ELISA) 

Participant Sample 1 Sample 2 Sample 3 Sample 4 LOD

Cry1Ab (MON810) 0.50% 2.00% 0.00% 0.00%

1895 P P N N Not Provided

Herculex 0.0% 1.00% 0.20% 0.00%

1895 N P P N Not Provided

Cry3Bb1 

(MON863/MON88017) 1.20% 0.50% 0.20% 0.00%

1895 P P N N Not Provided

Table 51: Percentage of correct results in the detection of biotechnology-derived

events in corn using Protein Based Enzyme-Linked Immunosorbent Assay (ELISA) 

Event Cry1Ab Herculex Cry3Bb1

Total # Reported results 4 4 4

# Incorrect 0 0 0

% Correct 100.0% 100.0% 100.0%

# Provided Positives 2 2 3

# False Negative 0 0 1

% False Negative 0.0% 0.0% 33.3%

# Provided Negatives 2 2 1

# False Positive 0 0 0

% False Positive 0.0% 0.0% 0.0%

Table 52: Results for soybeans fortified with CP4EPSPS for participants using 

Protein Based Enzyme-Linked Immunosorbent Assay (ELISA) 

CP4 EPSPS (RUR & RURII)

Participant Number

Sample 1

0.00%

Sample 2

0.50% LOD

2126 N P Not Provided
54

Table 53: Percentage of correct results in qualitative reports for CP4EPSPS for 

participants using Protein Based Enzyme-Linked Immunosorbent Assay (ELISA) 

Event CP4 EPSPS (RUR & RURII)

Total # Reported results 4

# Incorrect 0

% Correct 100.0%

# Provided Positives 3

# False Negative 0

% False Negative 0.0%

# Provided Negatives 1

# False Positive 0

% False Positive 0.0%

Table 54: Quantitative results and z-scores for corn fortified with T25 for all 

participants (DNA-based assays). Quantifications marked in red indicate value is an 

outlier determined by the “Grubb’s Test for Outliers”.

%w/w Fortification Level 0.0% 0.0% 0.8% 0.0%

Consensus Mean 0.0% 0.0% 0.53% 0.0%

Participant Number Result Result Result z-score Result

1754 0.00 0.00 0.70 0.58 0.00

1844 0.00 0.00 0.65 0.41 0.00

1870 0.00 0.00 0.85 1.07 0.00

1895 0.00 0.00 0.36 -0.55 0.00

2057 0.00 0.00 0.61 0.28 0.00

2716 0.00 0.00 2.90 0.00

2723 0.00 0.00 0.10 -1.41 0.00

2723* NR NR 0.28 -0.81 NR

4932 0.00 0.00 0.69 0.54 0.00

4952 0.00 0.00 0.40 -0.41 0.00

7017 0.00 0.00 0.10 -1.41 0.00

7030 0.00 0.00 1.04 1.70 NR

*-Digital PCR results

NR – Not Reported
55

Table 55: Quantitative results and z-scores for corn fortified with MON810 for all 

participants (DNA-based assays). Values highlighted in yellow indicate a z-score 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

%w/w Fortification Level 0.5% 2.0% 0.0% 0.0%

Consensus Mean 0.50% 1.89% 0.0% 0.0%

Participant Number Result z-score Result z-score Result Result

1752 0.25 -1.13 1.48 -0.61 0.00 0.00

1754 0.40 -0.46 1.20 -1.02 0.00 0.00

1764 0.25 -1.13 1.08 -1.20 0.00 0.00

1770 0.39 -0.51 1.32 -0.84 0.00 0.00

1844 0.41 -0.42 1.88 -0.02 0.00 0.00

1870 0.38 -0.55 1.90 0.01 0.00 0.00

1895 0.31 -0.86 1.09 -1.18 0.00 0.00

2005 0.89 1.71 2.87 1.43 0.00 0.00

2031 0.62 0.52 3.00 1.62 0.00 0.00

2057 0.53 0.12 1.90 0.01 0.00 0.00

2112 0.64 0.60 2.62 1.07 0.00 0.00

2716 0.46 -0.20 1.76 -0.20 0.00 0.00

2723 0.05 -2.02 0.70 -1.75 0.00 0.00

2723* 0.68 0.78 2.23 0.49 NR NR

2727 0.97 2.07 3.46 2.30 0.00 0.00

2732 P 2.34 0.65 0.00 0.00

3928 0.17 -1.48 0.85 -1.53 0.00 0.00

3929 0.54 0.16 1.62 -0.40 0.00 0.00

4504 0.75 1.09 1.20 -1.02 0.00 0.00

4936 0.44 -0.28 1.81 -0.12 0.00 0.00

4942 0.83 1.45 2.71 1.20 0.00 0.00

4952 0.70 0.87 2.50 0.89 0.00 0.00

4957 0.38 -0.55 1.36 -0.79 0.00 0.00

4960 0.31 -0.86 1.75 -0.21 0.00 0.00

4976 0.50 -0.02 2.30 0.60 0.00 0.00

7017 0.38 -0.55 1.80 -0.14 0.00 0.00

7026 0.74 1.05 2.18 0.42 0.00 0.00

7030 0.64 0.60 2.14 0.36 0.00 NR

*-Digital PCR results

NR – Not Reported
56

Table 56: Quantitative results and z-scores for corn fortified with GA21 for all 

participants (DNA-based assays). Values highlighted in yellow indicate z-scores 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier. 

Quantifications marked in red indicate values are an outlier determined by the “Grubb’s 

Test for Outliers”.

%w/w Fortification Level 0.0% 0.2% 0.5% 0.0%

Consensus Mean 0.0% 0.21% 0.57% 0.0%

Participant Number Result Result z-score Result z-score Result

1752 0.00 0.22 0.10 0.67 0.48 0.00

1754 0.00 0.20 -0.24 0.40 -0.85 0.00

1761 0.00 0.47 0.95 1.85 0.00

1764 0.00 0.25 0.62 0.37 -1.00 0.00

1770 0.00 0.14 -1.27 0.46 -0.56 0.00

1844 0.00 0.14 -1.27 0.56 -0.06 0.00

1870 0.00 0.22 0.10 0.48 -0.46 0.00

1895 0.00 0.21 -0.07 0.30 -1.34 0.00

2005 0.00 0.22 0.10 0.55 -0.11 0.00

2057 0.00 0.23 0.28 0.63 0.28 0.00

2716 0.00 0.73 0.90 1.61 0.00

2723 0.00 0.30 1.48 1.00 2.10 0.00

2723* NR 0.11 -1.78 0.34 -1.15 NR

2727 0.00 0.20 -0.24 0.51 -0.31 0.00

3928 0.00 0.16 -0.93 0.53 -0.21 0.00

4932 0.00 0.22 0.10 0.62 0.23 0.00

4936 0.00 <0.1 0.30 -1.34 0.00

4952 0.00 0.35 2.34 0.50 -0.36 0.00

4957 0.00 0.19 -0.41 0.42 -0.75 0.00

4974 0.00 0.50 0.90 1.61 0.00

4976 0.00 2.00 0.50 -0.36 0.00

7017 0.00 0.21 -0.07 0.57 -0.01 0.00

7030 0.00 0.28 1.14 0.72 0.72 NR

*-Digital PCR results

NR – Not Reported
57

Table 57: Quantitative results and z-scores for corn fortified with NK603 for all 

participants (DNA-based assays). Values highlighted in yellow indicate a z-score 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier. 

Quantifications marked in red indicate values are an outlier determined by the “Grubb’s 

Test for Outliers”.

%w/w Fortification Level 0.0% 0.0% 1.5% 0.0%

Consensus Mean 0.0% 0.0% 1.45% 0.0%

Participant Number Result Result Result z-score Result

1754 0.00 0.00 1.30 -0.43 0.00

1764 0.00 0.00 1.34 -0.31 0.00

1844 0.00 0.00 1.36 -0.26 0.00

1870 0.00 0.00 1.20 -0.72 0.00

1895 0.00 0.00 1.09 -1.03 0.00

2005 0.00 0.00 1.27 -0.52 0.00

2057 0.00 0.00 1.73 0.80 0.00

2112 0.00 0.00 1.60 0.43 0.00

2716 0.00 0.00 2.17 2.07 0.00

2723 0.00 0.00 10.00 0.00

2723* NR NR 2.10 1.87 NR

2727 0.00 0.00 1.70 0.72 0.00

2732 0.00 0.00 1.56 0.32 0.00

4504 0.00 0.00 1.50 0.14 0.00

4932 0.00 0.00 1.34 -0.31 0.00

4936 0.00 0.00 0.84 -1.75 0.00

4952 0.00 0.00 2.00 1.58 0.00

4957 0.00 0.00 1.45 0.00 0.00

4976 0.00 0.00 1.10 -1.00 0.00

7017 0.00 0.00 1.04 -1.18 0.00

7026 0.00 0.00 1.54 0.26 0.00

7030 0.00 0.00 1.21 -0.69 NR

*-Digital PCR results

NR – Not Reported
58

Table 58: Quantitative results and z-scores for corn fortified with Herculex® for all 

participants (DNA-based assays). Values highlighted in yellow indicate z-scores 

outside of the expected range, i.e., z > +2 or z < -2. Quantifications marked in red indicate

values determined to be either: (1) a positive value for a non-fortified sample (i.e. a false 

positive result); or (2) a negative value for a fortified sample (i.e. a false negative result); 

or (3) an outlier determined by the “Grubb’s Test for Outliers”.

%w/w Fortification Level 0.0% 1.0% 0.2% 0.0%

Consensus Mean 0.0% 0.47% 0.13% 0.0%

Participant Number Result Result z-score Result z-score Result

1754 0.00 0.50 0.18 0.10 -0.80 0.00

1764 0.00 0.62 0.89 0.13 0.15 0.00

1844 0.00 0.56 0.53 0.15 0.79 0.00

1870 0.00 0.46 -0.05 0.15 0.79 0.00

1895 0.00 0.55 0.47 0.16 1.10 0.00

2005 0.00 0.51 0.24 0.15 0.79 0.00

2057 0.00 0.56 0.53 0.19 2.06 0.00

2716 0.00 0.18 -1.70 0.10 -0.80 0.00

2723 0.00 0.13 -1.99 0.00 0.00

2723* NR 0.00 NR NR

2727 0.00 0.73 1.53 0.00 0.00

2732 0.00 0.58 0.65 NR 0.00

3928 0.00 0.25 -1.28 0.10 -0.80 0.00

4504 0.00 0.40 -0.40 0.44 0.00

4936 0.00 0.34 -0.76 0.10 -0.80 0.00

4945 0.00 0.75 1.65 0.11 -0.49 0.00

4952 0.00 0.40 -0.40 0.10 -0.80 0.00

4957 0.00 0.50 0.18 0.11 -0.49 0.00

4976 0.00 0.40 -0.40 0.10 -0.80 0.00

7017 0.00 0.41 -0.35 0.10 -0.80 0.00

7026 0.00 0.72 1.47 0.18 1.74 0.00

7030 0.00 0.30 -0.99 0.10 -0.80 NR

*-Digital PCR results

NR – Not Reported
59

Table 59: Quantitative results and z-scores for corn fortified with MON863 for all 

participants (DNA-based assays). Values highlighted in yellow indicate z-scores 

outside of the expected range, i.e., z > +2 or z < -2. Quantifications marked in red indicate 

values determined to be either: (1) a positive value for a non-fortified sample (i.e. a false 

positive result); or (2) a negative value for a fortified sample (i.e. a false negative result); 

or (3) an outlier determined by the “Grubb’s Test for Outliers”.

%w/w Fortification Level 1.0% 0.0% 0.2% 0.0%

Consensus Mean 1.23% 0.0% 0.17% 0.0%

Participant Number Result z-score Result Result z-score Result

1752 1.15 -0.14 0.00 0.10 -1.33 0.00

1754 1.30 0.13 0.00 0.20 0.57 0.00

1764 1.85 1.10 0.00 0.17 0.00 0.00

1844 1.01 -0.39 0.00 0.13 -0.76 0.00

1870 0.85 -0.67 0.00 0.13 -0.76 0.00

1895 0.83 -0.70 0.00 0.00 0.00

2005 1.63 0.71 0.00 0.13 -0.76 0.00

2057 1.35 0.21 0.00 0.19 0.38 0.00

2694 P 0.00 0.22 0.95 0.00

2716 2.57 2.37 0.00 0.25 1.52 0.00

2723 0.50 -1.29 0.00 0.00 0.00

2723* 1.82 1.04 NR NR NR

2727 0.68 -0.97 0.00 0.21 0.76 0.00

2732 1.05 -0.32 0.00 NR 0.00

3928 0.76 -0.83 0.00 0.17 0.00 0.00

4504 0.13 -1.94 0.00 0.89 0.00

4936 0.93 -0.53 0.00 0.12 -0.95 0.00

4952 1.80 1.01 0.00 0.20 0.57 0.00

4957 P 1.41 0.13 -0.76 0.00

4960 1.71 0.85 0.00 0.13 -0.76 0.00

4976 1.00 -0.40 0.00 0.30 2.48 0.00

7017 1.08 -0.26 0.00 0.14 -0.57 0.00

7030 1.80 1.01 0.00 0.14 -0.57 NR

*-Digital PCR results

NR – Not Reported

N - Fortification level/Consensus Mean below Participants’ LOD, assessed as Provided 

Negative

P - Qualitative data only
60

Table 60: Quantitative results and z-scores for corn fortified with Herculex® RW for 

all participants (DNA-based assays). Values highlighted in yellow indicate a z-score 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

%w/w Fortification Level 0.5% 0.0% 1.0% 0.0%

Consensus Mean 1.12% 0.0% 1.74% 0.0%

Participant Number Result z-score Result Result z-score Result

1754 0.90 -0.47 0.00 2.00 0.41 0.00

1764 1.61 1.06 0.00 1.45 -0.47 0.00

1844 0.74 -0.82 0.00 1.28 -0.74 0.00

1870 0.93 -0.41 0.00 1.18 -0.90 0.00

1895 0.21 -1.97 0.00 0.73 -1.62 0.00

2057 0.98 -0.30 0.00 1.30 -0.71 0.00

2716 2.05 2.02 0.00 3.37 2.60 0.00

2723 1.50 0.83 0.00 2.30 0.89 0.00

2723* 1.72 1.30 NR 2.35 0.97 NR

2727 1.38 0.57 0.00 1.91 0.27 0.00

2732 P 0.00 2.11 0.59 0.00

3928 0.95 -0.37 0.00 2.07 0.52 0.00

4936 0.87 -0.54 0.00 1.52 -0.36 0.00

4957 1.10 -0.04 0.00 1.57 -0.28 0.00

7017 0.75 -0.80 0.00 1.25 -0.79 0.00

7030 1.09 -0.06 0.00 1.51 -0.37 NR

*-Digital PCR results

NR – Not Reported
61

Table 61: Quantitative results and z-scores for corn fortified with MIR604 for all 

participants (DNA-based assays). Values highlighted in yellow indicate a z-score 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

%w/w Fortification Level 0.0% 0.0% 0.5% 0.0%

Consensus Mean 0.0% 0.0% 0.37% 0.0%

Participant Number Result Result Result z-score Result

1752 0.00 0.00 0.43 0.41 0.00

1754 0.00 0.00 0.20 -1.23 1.00

1761 0.00 0.00 0.56 1.33 0.00

1764 0.00 0.00 0.40 0.19 0.00

1770 0.00 0.00 0.50 0.90 0.00

1844 0.00 0.00 0.56 1.33 0.00

1870 0.00 0.00 0.36 -0.09 0.00

1895 0.00 0.00 0.27 -0.74 0.00

2005 0.00 0.00 0.34 -0.24 0.00

2057 0.00 0.00 0.54 1.19 0.00

2716 0.00 0.00 0.10 -1.95 0.00

2723 0.00 0.00 0.14 -1.66 0.00

2723* NR NR 0.40 0.19 NR

2727 0.00 0.00 0.09 -2.02 0.00

4504 0.00 0.00 0.45 0.55 0.00

4936 0.00 0.00 0.40 0.19 0.00

4952 0.00 0.00 0.50 0.90 1.00

4957 0.00 0.00 0.43 0.41 0.00

4974 0.00 0.00 0.30 -0.52 0.00

4976 0.00 0.00 0.40 0.19 0.00

7017 0.00 0.00 0.48 0.76 0.00

7030 0.00 0.00 0.36 -0.09 NR

*-Digital PCR results

NR – Not Reported
62

Table 62: Quantitative results and z-scores for corn fortified with Event 3272 for all 

participants (DNA-based assays). Values highlighted in yellow indicate a z-score 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

%w/w Fortification Level 1.0% 0.0% 0.0% 0.0%

Consensus Mean 1.23% 0.0% 0.0% 0.0%

Participant Number Result z-score Result Result Result

1752 1.27 0.14 0.00 0.00 0.00

1754 1.30 0.24 0.00 0.00 0.00

1764 1.37 0.49 0.00 0.00 0.00

1844 1.22 -0.04 0.00 0.00 0.00

1870 1.12 -0.40 0.00 0.00 0.00

1895 1.23 0.00 0.00 0.00 0.00

2057 1.46 0.81 0.00 0.00 0.00

2716 0.71 -1.85 0.00 0.00 0.00

2723 1.20 -0.11 0.00 0.00 0.00

2723* 1.40 0.60 NR NR NR

4932 0.90 -1.18 0.00 0.00 0.00

4936 1.08 -0.54 0.00 0.00 0.00

4952 2.00 2.74 0.00 0.00 0.00

4976 1.20 -0.11 0.00 0.00 0.00

7017 1.04 -0.68 0.00 0.00 0.00

7030 0.98 -0.89 0.00 0.00 NR

7032 1.45 0.78 0.00 0.00 0.00

*-Digital PCR results

NR – Not Reported
63

Table 63: Quantitative results and z-scores for corn fortified with MON 88017 for all 

participants (DNA-based assays). Values highlighted in yellow indicate a z-score 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

%w/w Fortification Level 0.2% 0.5% 0.0% 0.0%

Consensus Mean 0.34% 0.74% 0.0% 0.0%

Participant Number Result z-score Result z-score Result Result

1752 0.14 -0.98 0.35 -1.11 0.00 0.00

1754 0.20 -0.69 0.50 -0.68 0.00 0.00

1764 0.32 -0.10 0.85 0.33 0.00 0.00

1844 0.28 -0.30 0.65 -0.25 0.00 0.00

1870 0.20 -0.69 0.64 -0.27 0.00 0.00

1895 0.12 -1.08 0.30 -1.25 0.00 0.00

2005 0.25 -0.44 0.79 0.16 0.00 0.00

2057 0.38 0.20 0.95 0.62 0.00 0.00

2716 0.64 1.48 1.57 2.40 0.00 0.00

2723 0.70 1.77 1.50 2.20 0.00 0.00

2723* 0.43 0.44 0.81 0.22 NR NR

2732 NR 0.90 0.47 0.00 0.00

4932 0.50 0.79 0.68 -0.16 0.00 0.00

4936 0.20 -0.69 0.39 -0.99 0.00 0.00

4952 0.25 -0.44 0.45 -0.82 0.00 0.00

4957 0.23 -0.54 0.55 -0.53 0.00 0.00

4976 0.20 -0.69 0.60 -0.39 0.00 0.00

7017 0.25 -0.44 0.49 -0.71 0.00 0.00

7030 0.83 2.41 1.00 0.76 0.00 NR

* -Digital PCR results

NR – Not Reported
64

Table 64: Quantitative results and z-scores for corn fortified with MON 89034 for all 

participants (DNA-based assays). Z-scores outside of the expected range, i.e., z > +2 

or z < -2 were not observed in this data set.

%w/w Fortification Level 0.5% 0.0% 0.0% 0.0%

Consensus Mean 0.51% 0.0% 0.0% 0.0%

Participant Number Result z-score Result Result Result

1752 0.54 0.10 0.00 0.00 0.00

1754 0.30 -0.87 0.00 0.00 0.00

1764 0.66 0.59 0.00 0.00 0.00

1844 0.28 -0.95 0.00 0.00 0.00

1870 0.90 1.56 0.00 0.00 0.00

1895 0.12 -1.60 0.00 0.00 0.00

2005 0.36 -0.63 0.00 0.00 0.00

2057 0.66 0.59 0.00 0.00 0.00

2716 0.48 -0.14 0.00 0.00 0.00

2723 0.90 1.56 0.00 0.00 0.00

2723* 0.90 1.56 NR NR NR

2732 0.69 0.71 0.00 0.00 0.00

3928 0.30 -0.87 0.00 0.00 0.00

4932 0.63 0.47 0.00 0.00 0.00

4936 0.22 -1.19 0.00 0.00 0.00

4952 0.30 -0.87 0.00 0.00 0.00

7017 0.29 -0.91 0.00 0.00 0.00

7026 0.50 -0.06 0.00 0.00 0.00

7030 0.75 0.95 0.00 NR NR

* -Digital PCR results

NR – Not Reported
65

Table 65: Quantitative results and z-scores for corn fortified with MIR162 for all 

participants (DNA-based assays). Values highlighted in yellow indicate z-scores 

outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

%w/w Fortification Level 0.0% 1.0% 0.0% 0.0%

Consensus Mean 0.0% 1.79% 0.0% 0.0%

Participant Number Result Result z-score Result Result

1752 0.00 1.99 0.27 0.00 0.00

1754 0.00 0.80 -1.39 0.00 0.00

1761 0.00 1.83 0.05 0.00 0.00

1764 0.00 2.65 1.20 0.00 0.00

1770 0.00 2.05 0.36 0.00 0.00

1844 0.00 1.74 -0.08 0.00 0.00

1870 0.00 1.00 -1.11 0.00 0.00

1895 0.00 1.04 -1.06 0.00 0.00

2005 0.00 2.35 0.78 0.00 0.00

2057 0.00 2.87 1.51 0.00 0.00

2716 0.00 1.77 -0.03 0.00 0.00

2723 0.00 3.50 2.39 0.00 0.00

2723* NR 1.67 -0.17 NR NR

2732 0.00 1.75 -0.06 0.00 0.00

4936 0.00 1.42 -0.52 0.00 0.00

4952 0.00 2.60 1.13 0.00 0.00

4957 0.00 1.35 -0.62 0.00 0.00

4974 0.00 0.70 -1.53 0.00 0.00

4976 0.00 2.00 0.29 0.00 0.00

7017 0.00 1.13 -0.93 0.00 0.00

7030 0.00 1.46 -0.47 0.00 NR

*-Digital PCR results

NR – Not Reported
66

Table 66: Quantitative results and z-scores for soybeans fortified with CP4 EPSPS 

as Roundup ReadyTM (RUR) for all participants (DNA-based assays). Quantifications 

marked in red indicate values determined to be either: (1) a positive value for a nonfortified sample (i.e. a false positive result); or (2) a negative value for a fortified sample 

(i.e. a false negative result); or (3) an outlier determined by the “Grubb’s Test for Outliers”. 

Values highlighted in yellow indicate a z-score outside of the expected range, i.e., z > +2 

or z < -2 that is not considered an outlier.

%w/w Fortification Level 0.0% 0.5%

Consensus Mean 0.0% 0.43%

Participant Number Result Result z-score

1754 0.00 0.50 0.63

1761 0.00 0.42 -0.06

1764 0.00 0.22 -1.77

1770 0.00 0.58 1.31

1782 0.00 0.50 0.63

1785 0.00 0.35 -0.66

1788 0.00 0.35 -0.66

1844 0.00 0.54 0.97

1870 0.00 0.50 0.63

2005 0.00 0.46 0.28

2031 0.00 0.30 -1.09

2057 0.00 0.43 0.03

2060 <LOD 0.43 0.03

2067 0.00 0.37 -0.49

2691 0.00 0.35 -0.66

2692 0.00 0.32 -0.91

2716 0.00 0.32 -0.91

2723 0.00 0.70 2.34

2723* NR 0.60 1.48

2727 0.00 0.31 -1.00

3949 NQ 1.01

4504 0.00 0.66 2.00

4901 0.00 0.48 0.45

4932 0.00 0.63 1.74

4935 0.00 0.40 -0.23

4936 0.00 0.35 -0.66

4942 0.00 0.36 -0.57

4952 0.00 0.40 -0.23

4957 0.62 0.00

4960 0.00 0.32 -0.91

4976 0.00 0.40 -0.23

4978 0.00 0.29 -1.17

7017 0.00 0.35 -0.66

7025 0.00 0.89
67

%w/w Fortification Level 0.0% 0.5%

Consensus Mean 0.0% 0.43%

Participant Number Result Result z-score

7030 NR 0.47 0.37

*-Digital PCR results

NR – Not Reported

Table 67: Quantitative results and z-scores for soybeans fortified with A2704-12 

(Liberty Link®) for all participants (DNA-based assays). Quantifications marked in 

red indicate values determined to be either: (1) a positive value for a non-fortified sample 

(i.e. a false positive result); or (2) a negative value for a fortified sample (i.e. a false 

negative result); or (3) an outlier determined by the “Grubb’s Test for Outliers”.

%w/w Fortification Level 0.0% 0.5%

Consensus Mean 0.0% 0.41%

Participant Number Result Result z-score

1754 0.00 0.50 0.52

1761 0.00 0.39 -0.08

1764 0.00 0.23 -0.96

1770 0.00 0.41 0.03

1844 0.00 0.32 -0.47

1870 0.00 0.43 0.14

1895 0.00 0.94 2.93

2005 0.00 0.34 -0.36

2031 0.00 0.35 -0.30

2057 0.00 0.31 -0.52

2067 0.00 0.39 -0.08

2716 0.00 1.17

2723 0.00 0.00

4932 0.00 0.44 0.19

4952 0.00 0.20 -1.12

4960 0.00 0.42 0.08

4976 0.00 0.40 -0.03

4978 0.00 0.11 -1.62

7017 0.00 0.30 -0.58

7025 0.00 0.68 1.50

7030 NR 0.54 0.74

*-Digital PCR results

NR – Not Reported
68

Table 68: Quantitative results and z-scores for soybeans fortified with CP4 EPSPS 

as Roundup ReadyTM II (RUR II) for all participants (DNA-based assays).

%w/w Fortification Level 0.0% 0.0%

Consensus Mean 0.0% 0.0%

Participant Number Result Result

1754 0.00 0.00

1761 0.00 0.00

1764 0.00 0.00

1770 0.00 0.00

1782 0.00 0.00

1844 0.00 0.00

1870 0.00 0.00

2031 0.00 0.00

2057 0.00 0.00

2067 0.00 0.00

2716 0.00 0.00

2723 0.00 0.00

4901 0.00 0.00

4932 0.00 0.00

4935 0.00 0.00

4936 0.00 0.00

4952 0.00 0.00

4960 0.00 0.00

4976 0.00 0.00

4978 0.00 0.00

7017 0.00 0.00

7025 0.00 0.00

7034 0.00 0.00
69

Table 69: Quantitative results and z-scores for soybeans fortified with DP305423 

for all participants (DNA-based assays). Values highlighted in yellow indicate a zscore outside of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

%w/w Fortification Level 0.0% 1.0%

Consensus Mean 0.0% 0.37%

Participant Number Result Result z-score

1754 0.00 0.40 0.22

1764 0.00 0.31 -0.43

1844 0.00 0.36 -0.07

1870 0.00 0.50 0.94

2031 0.00 0.26 -0.79

2057 0.00 0.29 -0.57

2067 0.00 0.39 0.15

2716 0.00 0.10 -1.95

2723 0.00 0.20 -1.23

2723* NR 0.31 -0.43

4504 0.00 0.73 2.61

4932 0.00 0.41 0.29

4936 0.00 0.45 0.58

4952 0.00 0.30 -0.50

4976 0.00 0.40 0.22

7017 0.00 0.44 0.51

7030 0.00 0.52 1.09

7032 0.00 0.28 -0.65

* -Digital PCR results

NR – Not Reported
70

Table 70: Quantitative results and z-scores for 35S, NOS, and FMV in corn for all 

participants (DNA based assay). Value highlighted in yellow indicates a z-score outside 

of the expected range, i.e., z > +2 or z < -2 that is not considered an outlier.

Event: 35S

Consensus Mean 1.8% 1.6% 2.0% 0.0%

Participant Number Result z-score Result z-score Result z-score Result

1785 2.00 0.20 1.61 -0.03 1.26 -0.77 0.00

1844 2.00 0.20 1.90 0.42 2.37 0.35 0.00

1895 0.56 -1.18 0.63 -1.52 0.41 -1.63 0.00

2720 1.90 0.10 1.93 0.46 3.36 1.35 0.00

3929 1.58 -0.21 1.32 -0.47 1.62 -0.41 0.00

3949 4.29 2.38 2.99 2.07 3.43 1.42 NQ

4936 1.43 -0.35 1.26 -0.56 2.35 0.33 0.00

4964 1.30 -0.47 1.20 -0.65 1.30 -0.73 <0.01

4974 1.10 -0.66 1.80 0.26 2.10 0.08 0.00

Event: NOS

Consensus mean 1.4% 1.8% 2.6% 0.0%

Participant Number Result z-score Result z-score Result z-score Result

1785 1.84 1.07 2.67 1.54 3.73 1.33 0.00

1844 1.78 0.92 1.83 0.00 3.33 0.86 0.00

1895 0.94 -1.22 1.62 -0.38 1.12 -1.72 0.00

2720 0.92 -1.27 1.27 -1.02 2.27 -0.38 0.00

3929 1.66 0.61 1.52 -0.57 2.41 -0.22 0.00

4964 1.20 -0.56 1.40 -0.79 2.30 -0.34 0.00

4974 1.60 0.46 2.50 1.23 3.00 0.47 0.00

Event: FMV

Consensus mean 0.3% 0.0% 0.0% 0.0%

Participant Number Result z-score Result Result Result

1895 0.21 -0.71 0.00 0.00 0.00

4936 0.30 0.71 0.00 0.00 0.00

Note: Levels cited as Consensus Mean are averages of reported quantitative results of 

events containing these promoters and terminators. Assigning a more accurate 

quantification value is beyond the scope of this program at this time.
71

Table 71: Quantitative results and z-scores for 35S, NOS, and FMV in soybean for 

all participants (DNA based assay). Z-scores outside of the expected range, i.e., z > 

+2 or z < -2 were not observed in this data set. The sample size for event FMV soybean 

are too small to be statistically significant.

35S Soy

Consensus mean 0.0% 1.1%

Participant Number Result Result z-score

1844 0.00 1.23 0.22

1862 0.00 0.69 -1.11

1895 0.00 1.77 1.56

2720 0.00 0.59 -1.36

3887 0.00 1.40 0.64

4936 0.00 1.10 -0.10

4964 0.00 1.20 0.15

NOS Soy

Consensus mean 0.0% 0.6%

Participant Number Result Result z-score

1895 0.00 0.71 0.71

4964 0.00 0.4 -0.71

FMV Soy

Participant Number Sample 1 Sample 2

1844 0.00 0.0

1895 0.00 0.0

2723 0.00 0.0

Note: Levels cited as Consensus Mean are averages of reported quantitative results of 

events containing these promoters and terminators. Assigning a more accurate 

quantification value is beyond the scope of this program at this time.
72

Table 72: Quantitative results for corn fortified with biotechnology-derived events 

using Protein Based Lateral Flow Strip (LFS) tests. Quantifications marked in red

indicate values determined to be a negative value for a fortified sample (i.e. a false 

negative result).

Participant Number Sample 1 Sample 2 Sample 3 Sample 4

T 25 0.00% 0.00% 0.80% 0.00% LOD

1895 0.00 0.00 0.46 0.00 0.5%

EPSPS 

(NK603/MON88017) 0.2% 0.50% 1.50% 0.00%

1895 0.00* 0.00* 1.5 0.00 0.5%

Cry1Ab (MON810) 0.50% 2.00% 0.00% 0.00%

1895 0.00* 0.00 0.00 0.00 0.8%

Herculex 0.0% 1.00% 0.20% 0.00%

1895 0.00 0.00* 0.00* 0.00 0.5%

Cry3Bb1 

(MON863/MON88017) 1.20% 0.50% 0.20% 0.00%

1895 0.88 0.00* 0.00* 0.00 0.5%

Hclx RW 0.50% 0.00% 1.00% 0.00%

1895 0.85 0.00 0.95 0.00 0.5%

MIR 604 0.00% 0.00% 0.50% 0.00%

1895 0.00 0.00 0.7 0.00 1.0%

* - Fortification level/Consensus Mean below Participants’ LOD, assessed as Provided 

Negative

Table 73: Quantitative results for soybeans fortified with CP4 EPSPS as Roundup 

ReadyTM (RUR) and A2704-12 (Liberty Link) using Protein Based Lateral Flow Strip 

(LFS) tests

CP4 EPSPS (RUR & RURII) Sample 1 Sample 2

Participant Number 0.00% 0.50% LOD

1895 0.0 >3 0.25%

A2704-12 (Liberty Link) Sample 1 Sample 2 LOD

Participant Number 0.0% 0.5%

1895 0.0 1.5 0.5%

Table 74: Quantitative results for soybeans fortified with CP4 EPSPS as Roundup 

ReadyTM (RUR) using Protein Based Enzyme-Linked Immunosorbent Assay 

(ELISA)

Event: RUR/RUR II

%w/w Fortification Level 0.0% 0.5%

Participant Number Result Result LOD

1782 0.00 0.40 0.3%
73

Table 75: Descriptive statistics for participants reported quantifications relative to 

USDA/AMS/FGIS fortification levels using DNA-based assays. % Relative standard 

deviation (%RSDR) = [standard deviation/mean value x 100]. Outliers were determined 

by the Grubb’s Test for Outliers and excluded from calculations involving reported mean, 

standard deviation, and % relative deviation but were included in the range of results.

Biotechnologyderived EventReported 

Results 

(N)

Gravimetric 

Fortification 

(%w/w)

Consensus 

Mean

Standard 

Deviation

% 

Relative 

Standard 

Deviation

Range of 

Results (%)

T25 12 0.8 0.53 0.30 56.6 0.10 - 2.9

MON810 27 0.5 0.50 0.23 46.0 0.05 - 0.97

MON810 28 2.0 1.89 0.68 36.0 0.70 - 3.46

GA21 22 0.2 0.21 0.06 28.6 0.11 - 2.0

GA21 23 0.5 0.57 0.20 35.1 0.3 - 1.00

NK603 22 1.5 1.45 0.35 24.1 0.84 - 10.00

Herculex 20 0.2 0.13 0.03 23.1 0.0 - 0.44

Herculex 22 1.0 0.47 0.17 36.2 0.0 - 0.75

MON863 22 0.2 0.17 0.05 29.4 0.0 - 0.89

MON863 23 1.0 1.23 0.57 46.3 0.13 - 2.57

HerculexRW 16 0.5 1.12 0.46 41.1 0.21 - 2.05

HerculexRW 16 1.0 1.74 0.63 36.2 0.73 - 3.37

MIR604 22 0.5 0.37 0.14 37.8 0.09 - 0.56

EV3272 17 1.0 1.23 0.28 22.8 0.71 - 2.00

MON88017 18 0.2 0.34 0.20 58.8 0.12 - 0.83

MON88017 19 0.5 0.74 0.35 47.3 0.3 - 1.57

MON89034 19 0.5 0.51 0.25 49.0 0.12 - 0.9

MIR 162 21 1.0 1.79 0.71 39.7 0.70 - 3.5

RUR 35 0.5 0.43 0.12 27.9 0.00 - 1.01
74

Biotechnologyderived EventReported 

Results 

(N)

Gravimetric 

Fortification 

(%w/w)

Consensus 

Mean

Standard 

Deviation

% 

Relative 

Standard 

Deviation

Range of 

Results (%)

LL 21 0.5 0.41 0.18 43.9 0.00 - 1.17

DP305424 18 1.0 0.37 0.14 37.8 0.10 - 0.73 
75

Summary of Findings

It is recognized that some organizations participate in this program to retain their ISO 

accreditation. Participation also serves to provide a verification of current laboratory 

practices and/or aids in identifying areas for improvement. These factors should be taken 

into consideration when reviewing the following analyses.

USDA/AMS/FGIS does not dictate detection methods for biotechnology-derived traits. An 

individual laboratory may provide a Limit of Detection (LOD) for its particular detection 

method. When the consensus mean in an event-fortified sample falls below the provided 

LOD, a negative result is considered a correct answer, and is assessed as a provided 

negative.

• Qualitative Sample Analysis

DNA-based Testing. The typical method of DNA-based testing for qualitative 

determination of events is by conventional PCR which generally has a sensitivity of 

0.01% w/w biotechnology-derived event. This level is consistent with what has been 

reported by Lipp et al. and represents the lowest concentration of genetic material that 

can be reliably detected by qualitative PCR.

The lowest gravimetric fortification level in this round of proficiency testing was 0.2% 

w/w; therefore, if the event was present it should be detectible by a laboratory that 

employs conventional PCR. As evidenced by the summary of performance scores 

(Table 45 and Figure 1), all of the sixteen (16) biotechnology-derived events were 

correctly detected with greater than 90% except T25, and fourteen (14) of the sixteen 

(16) biotechnology-derived events were correctly detected with greater than or equal 

to 95% reliability, and one (1) of the sixteen (16) biotechnology-derived events were 

correctly detected with 100% reliability.

Eleven (11) participants reported using an End-point PCR test method. Five (5)

participants reported using Digital PCR. 

Protein-based Testing. The methods of protein-based testing were lateral flow strip 

(LFS) and enzyme-linked immunosorbent assay (ELISA). The LFS test has a 

sensitivity ranging between 0.1- 2.0% w/w for corn events and 0.1- 0.50% w/w for 

soybean events according to some kit manufacturers. Generally, ELISA has a 

sensitivity of 0.1- 1.0% w/w for corn and soy events (Ahmed, 2004). The test results 

for MON863 and MON88017 are combined because both events express the C3Bb1

protein, and protein-based testing cannot distinguish between the two events. The 

event Herculex® contains the pat (phosphinothricin N-transferase) gene, but 

combining the results is problematic.

• Quantitative Sample Analysis

DNA-based Testing. The typical method of DNA-based testing for quantitative 

determination of biotechnology-derived events is by real-time quantitative PCR 
76

(qPCR). This analytical method has a limit of detection (LOD) of 0.01% w/w and a limit 

of quantification (LOQ) of approximately 0.1% w/w for a specified event (Ahmed, 2004; 

Lipp et al., 2005). 

Composite Performance Assessment. These data combined the participants’ 

reported quantifications and evaluated the group’s performance by considering the 

mean value of “reported results” of all participants (Table 75). Because test samples 

were fortified ranging from 0.2- 2.0 % w/w of a particular event, it was expected that 

qPCR technologies would detect the traits in all of the fortified samples but not in nonfortified samples. Low instances of false positive results were observed when using 

qPCR to detect the presence of Biotechnology-derived traits in these proficiency 

samples.

Nine (9) participants submitted quantitative results for 35S event in corn, seven (7) 

participants’ submitted quantitative results for NOS event, and two (2) participants’ 

submitted quantitative results for FMV event (Table 70). The target %w/w levels for 

these traits should be additive for all events containing either 35S or NOS fortified into 

the sample. Validated methods to quantify with a high degree of accuracy for the 

presence of 35S and NOS, in samples fortified with multiple traits, is beyond the scope 

of the program at this time. The number of results for soybean NOS and FMV are too 

small to hold statistical significance.

Seven (7) laboratories submitted quantitative results for the 35S event in soybean, two

(2) laboratories submitted quantitative results for the NOS event, and three (3) 

laboratory submitted quantitative results for the FMV event (Tables 71).

One laboratory submitted Quantitative results for corn fortified with biotechnologyderived events using Lateral Flow Strip (LFS) testing (Table 72).

One laboratory submitted Quantitative results for soybeans fortified with CP4 EPSPS 

as Roundup ReadyTM (RUR) and A2704-12 (l9berty Link) using Lateral Flow Strip 

(LFS) testing (Table 73).

One laboratory submitted Quantitative results for soybeans fortified with CP4 EPSPS 

as Roundup ReadyTM (RUR) using Protein Based Enzyme-Linked Immunosorbent 

Assay (ELISA) (Table 74).

Quantitative data from previous rounds of our proficiency sample distributions can be 

found at: http://www.gipsa.usda.gov/fgis/proficiencyprogram.aspx

Individual Performance Assessment. The performance of each participating 

laboratory for quantifying biotechnology-derived events in the proficiency samples can 

be observed by inspecting Tables 54 through 74. To assess the accuracy of their 

reported quantifications z-scores were computed. Laboratories with z-scores above 

+2 or below -2 were noted and highlighted in yellow because their result was greater 

than two standard deviations from the target value. Interpretation of z-scores assumes 

that the data have a normal distribution. Data from samples with lower fortification 
77

levels (e.g., 0.2% w/w) and from tables with low numbers of results are not normally 

distributed and caution should be used when interpreting their z-scores.

Monitoring and improving the performance of laboratories that use PCR technologies 

for the detection and/or quantification of biotechnology-derived events in corn and 

soybeans will improve the reliability of testing methods and the marketing of these 

commodities. The USDA/AMS/FGIS proficiency testing program should be a 

complement to other quality assurance measures that laboratories use to improve their

analytical capabilities.

Note: The biotechnology-derived seed or grain used to prepare these samples 

was made available to USDA/AMS/FGIS by the Life Science Organizations. Care 

was taken to ensure the biotechnology-derived material was either essentially 

100% positive for the event, or adjusted accordingly. The fortified samples were 

prepared using a process that has been verified to produce homogenous mixes, 

and representative samples were analyzed to ensure proper fortification and 

homogeneity. Reference standards are now commercially available for all 

biotechnology-derived traits used in this proficiency program and 

USDA/AMS/FGIS encourages the use of these reference materials when 

developing internal validated methods.

To obtain additional information on the USDA/AMS/FGIS Proficiency Program, contact 

Dr. Brian Beecher at US 816-891-0453, or by e-mail at Brian.S.Beecher@usda.gov or 

Dr. Ramaswamy Mani at US 816-891-0418, or by e-mail at 

Ramaswamy.Mani@usda.gov. For questions regarding this report, contact Dr. 

Ramaswamy Mani at US 816-891-0418, or by e-mail at Ramaswamy.Mani@usda.gov.

References

National Institute of Standards and Technology, Statistical Engineering Division, 

Consensus Mean

Ahmed, F.E. (editor.). 2004. Testing of genetically modified organisms in foods. The 

Haworth Press, Inc., Binghampton, NY, pp. 174-176.

Definition of minimum performance requirements for analytical methods in GMO testing. 

13 October 2008. European Network of GMO Laboratories and the Joint Research 

Center. 

Lipp, M., Shillito, R., Giroux, R., Spiegelhalter, F., Charlton, S., Pinero, D. 2005. 

Polymerase chain reaction technology as an analytical tool in agricultural biotechnology. 

Journal of AOAC International, 88 (1), 136-154.
78

List of organizations who wished to be identified as a participant in the USDA/AMS/FGIS

October 2017 Proficiency Program. Participant identification numbers are listed below 

with permission from the organization.

Agricultural Biotechnology Research 

Institute

Ayub Agricultural Research Institute (AARI)

Faislabad, Punjab-38950

Pakistan

Attn: Dr. Shahid Nazir

Phone: 092-41-9021669

shahidnazir97@yahoo.com

ANSES-Laboratoire de la santé des 

végétaux

7 rue Jean Dixméras

49044 ANGERS CEDEX

France

Attn: David Pornin

Phone: +33 2 41 20 74 55

david.pornin@anses.fr

BASF Plant Science

26 Davis Drive

Research Triangle Park, NC 27713

USA

Attn: Connie Martin

Phone: +1 919-547-2539

connie.martin@basf.com

BioGEVES

Station Expérimentale du Magneraud

CS 40052

Surgeres 17700

France

Attn: Fouilloux Stephane

Phone: 33-(0)546-683-036

stephane.fouilloux@geves.fr

BIOTECON Diagnostics GmbH

Hermannswerder 17

14473 Potsdam

Germany

Attn: Dr. Hans-Henno Dörries

Phone: +49 331 2300 211

hdoerries@bc-diagnostics.com

Bolsa de Comercio de Rosario

Cordoba 1402

Rosario -Sante Fe, S2000AWV

Argentina

Attn: Ariel Soso / Cesar Olsina

Phone: 54-341-5258300

asoso@bcr.com.ar / colsina@bcr.com.ar

Bureau of Quality and Safety of Foods

Tiwanon Road

Ministry of Public Health

Nanthaburi 11000

Thailand

Attn: Nittaya Phunbua

Phone: 662-9510000 ext 99514

nittaya.ph@dmsa.mail.go.th

Canadian Grain Commission

1404-303 Main Street

Winnipeg, MB R3C 3G8

Canada

Attn: Tigst Demeke

Phone: 204-984-4582

tigst.demeke@grainscanada.gc.ca

Cargill METNA (Orhangazi Corn Milling 

Plant)

Gemiç Köyü Karapınar Mevkii

16840 Orhangazi Bursa

Turkey

Attn: Osman Karabulut

Phone: 90 224 219 70 00

osman_karabulut@cargill.com
79

Central Laboratory (Thailand) Co., Ltd.

Bangkok Branch

50 Phaholyothin Rd.

Ladyao, Jatujak, Bangkok 10900

Thailand

Attn: MissThanida Pimma / Mrs.Kanittha 

Chaleekul

Phone: 66 2 940 6881-3, ext.210, 227

thanida@centrallabthail.com / qmbk@centrallabthai.com

Centro de Biología Molecular

Universidad Centroamericana, UCA.

Pista JP Segundo. Rotonda R Darío

150 mts Oeste, Managua Zip code: 69

Nicaragua

Attn: MsC. Lucía Paiz Medina

Phone: 505-22783923 ext 1315

luciapaiz@uca.edu.ni

Centro de Investigacion y Asitencia en 

Tecnologia y Diseno del Estado de 

Jalisco

Av. Normalistas No. 800 Colonia Colinas de 

la Normal CP 44270

Guadalajara, Jalisco

Mexico

Attn: Elsa Leticia Ramirez Cerda

Phone: +52(33)33 45 52 00 ext 1820

eramirez@ciatej.mx

Centro Nacional de Alimentacion 

(AESAN)

Ctra. Pozuelo-Majadahonda km 5.1

C.P.28220 (Majadahonda) Madrid

Spain

Attn: Isabel Prieto Santos

Phone: +34 91 338 06 88

mprietos@msssi.es

Certified Laboratories of the Midwest

175 E. Crossroads PKWY

Suite F

Bolingbrook, IL 60440

Attn: Neil Rogman

Phone: 630-783-8600

nrogman@certified-laboratories.com

CSP Labs

3556 Sankey Rd

Pleasant Grove, CA 95668

USA

Attn: Avninder Padda

Phone: 916-655-1581

avninder.padda@csplabs.com

1752

CNTA-Laboratorio del Ebro

Carretera NA- 134 km 53

31570 San Adrian, Navarra

Spain

Attn: Javier Butron Ilundain

Phone: 00 34 948 670 159

jbutron@cnta.es

Control Union Paraguay S.A.

Asunción, 1229

Paraguay

Attn: Rubén Darío Duré / Clara Nuñez / 

Patricio Fraser

Phone: +595 21524195

laboratorio.paraguay@controlunion.com / 

pfraser@controlunion.com

Deibel Laboratories of WI

103 South Second Street

Madison, WI-53704

USA

Attn: Jill Tecalero

Phone: 608-241-1177

jtecalero@deibellabs.com
80

DuPont do Brasil S.A. - Divisão Pioneer 

Sementes

Unid. de Pesquisa e Beneficiamento -

Brasília – DF, Rodovia DF 250, KM 20

Núcleo Rural Santos Dumont, Lote 50 –

Planaltina, 73310-970

Brazil

Attn: Nilson Matheus Mattioni

Phone: +55 (61) 2106 -1008

nilson.mattioni@pioneer.com

Du Pont do Brasil S.A. – Divisão Pioneer 

Sementes

Rodovia BR 452, km 187, Cx. Postal 1014,

75503-970 Itumbiara – GO

Brazil

Attn: Monica Garcia

Phone: 55 64 3432 2677

monica.garcia@pioneer.com

Envirocare Labs Pvt. Ltd.

A7 Enviro House, MIDC Main Road,

Wagle Industrial EstateRiver Falls

Thane, Maharashtra 400604

India

Attn: Ms. Meenal Satghare

Phone: 919167232032

meenal.s@envirocare.co.in

7016

Eurofins BioDiagnostics

507 Highland Drive

River Falls, WI 54022

USA

Attn: Anna Doornink

Phone: 715-426-0246

AnnaDoornink@eurofinsUS.com

Eurofins Genescan, Inc.

2219 Lakeshore Drive

Suite 400

New Orleans, Louisiana 70122

USA

Attn: Gregory M. Ditta

Phone: 504-297-4356

gregoryditta@eurofinsus.com

Exact Scientific Services

1355 Pacific Place, Suite 101

Ferndale, WA 98248

USA

Attn: Amber Stoner

Phone: 360-733-1205

a.stoner@exactscientific.com

3888

Export Inspection Agency – Kochi 

Laboratory

27/1767 A, Shipyard Quarters Road,

Panampilly Nagar (South), Kochi –682036, 

Kerala

India

Attn: Dr. Anoop A. Krishnan

Phone: +91 484 2316945, 2316946, 

2316949

eia-kochilab@eicindia.gov.in

FASMAC CO., LTD

5-1-3 Midorigaoka, Atsugi-shi,

Kanagawa, 243-0041

Japan

Attn: Yukiko Yamakoshi

Phone: 81-46-295-8787

yyamakoshi@fasmac.co.jp

Federal Food Safety and Veterinary 

Office FSVO

Risk Assessment Division

Schwarzenburgstrasse 165

CH-3003 Bern

Switzerland

Attn: Dominik Moor

Phone: +41 58 464 93 78

Dominik.Moor@bag.admin.ch

FirstSource Laboratory Solutions(LLP)

First Floor, Plot No A1/B,

IDA Nacharam Cross Road

Hyderabad 500076

India

Attn: Visala Y

Phone: 8099920104

visalay@firstsourcels.com
81

Food and Daily Use Objects Analysis 

Laboratory

Voivodeship Sanitary-Epidemiological 

Station in Poznan

ul. Noskowskiego 23

61-705 Poznan

Poland

Attn: Bartosz Rogozinski

Phone: (0048) 061 8544 855

bartosz.rogozinski@vp.pl

Formul Darica Ozel Gida Laboratuvari

İstasyon Street.Number:423/9 Darıca 

KOCAELİ

zip code:41700

Turkey

Attn: Ugur Ozdemir

Phone: +90 0262 655 00 68

bilgi@formullab.com / 

ugurozdemir@formullab.com

7041

Icerenkoy Mh., Degirmenyolu

No:12 K:2-3 Atasehir,

Istanbul, 34752

Turkey

Attn: Merve Vural

Phone: +90 0216 576 38 38

info@formullab.com / 

mervevural@formullab.com

7040

Genetic Purity Lab Monsanto Argentina

Maipú 1210 piso 10

1006 Capital Federal

Argentina

Attn: Matías Salamé / Tatiana Günther / 

María Passadore

Phone: 54 1143162453

maria.passadore@monsanto.com / 

matias.salame@monsanto.com / 

tatiana.gunther@monsanto.com

GEN-IAL GmbH

Heuserweg 13 - 15

D- 53842 Troisdorf

Germany

Attn: Dr. Gabriele Mücher

Phone: *49 2241 2522981

g.muecher@gen-ial.de

GeoChem Laboratories Pvt. Ltd.

Pragati Building, adjacent to Crompton 

Greaves,

Kanjurmarg East, Mumbai-4000042

India

Attn: Mr. P. Sureshbabu / Ms. Sheetal Patil

Phone: +91 22 6195100

sureshbabu.p@geochem.net.in /

sheetal.p@geochem.net.in

4939

Germ Services

21, Chemin de Pau

64121 Montardon

France

Attn: Cedric Barbe-Barrailh / Pilar Cambet

Phone: 05-59-12-67-61

cedric.barbe@agpm.com / 

pilar.cambet@agpm.com

GET Lab @ Pioneer Hi-Bred

8325 NW 62nd Ave

Johnston, IA 50131

USA

Attn: Alicia Garrity

Phone: 515-535-6402

alicia.garrity@pioneer.com

ICAR-National Bureau of Plant Genetic 

Resources

Pusa Campus

New Delhi-110012

India

Attn: Dr. Gurinderjit Randhawa

Phone: 091-9811974396

gurinder.randhawa@rediffmail.cm /

gurinder.randhawa@icar.gov.in

4504
82

IEH

15300 Bothell Way NE

Lake Forest Park, WA 98155

USA

Attn: Cesar Nadala / Youngsil Ha

Phone: (206) 522-5432

cnadala@iehinc.com / yha@iehinc.com

ifp Institut für Produktqualität

Wagner-Regeny-Str. 8

12489 Berlin

Germany

Attn: Dr. Wolfgang Hauser

Phone: 0049(0)30-7473331013

hauser@produktqualitaet.com

Indiana Crop Improvement Association

7700 Stockwell Rd

Lafayette, IN 47909

USA

Attn: Dr. Emily Dierking

Phone: 765-523-2535

dierking@indianacrop.org

Ingredion Shanghai Analysis Lab

No 450 Hua Tie Road, Songjiang Industrial 

Estate,

Shanghai, 201600

PR China

Attn: Candy Zhu

Phone: +86 21 377 400 66 – 2260

candy.zhu@ingredion.com

4982

Instituto de Biotecnologia

Pedro de Alb a y Manuel L. Barragán S/N

Cd. Universitaria

San Nicolás de los Garza, NL. CP. 66450

Mexico

Attn: Dr. Benito Pereyra Alférez

Phone: (+52 81)83294000 Ext. 6438

bpereyra@gmail.com

4950

Instituto Nacional de Semillas

Venezuela 162, CP 1095 – Ciudad 

Autónoma de Buenos Aires - Argentina

Laboratorio de Marcadores Moleculares y 

Fitopatología

Argentina

Attn: Ana Vicario

Phone: 54 11 4349 2037

alvicario@inase.gov.ar

Intertek Scanbi diagnostics

Elevenborgsvagen 2

230 53 Alnarp

Sweden

Attn: Peo Gummeson

Phone: 46406928001

peo.gummeson@intertek.com

Iowa State University Seed Testing 

Laboratory

2115 Osborn Dr.

138 Seed Science Center

Ames, IA-50011

USA

Attn: Tyler Tunning

Phone: 515-294-6028

ttunning@iastate.edu

Istanbul Food Control Laboratory 

Dierctorate

Florya Asfaltı No:78

Bakırköy-İSTANBUL 34153

Turkey

Attn: Ferruh TOMBUL

Phone: +90 212 663 39 61

ferruh.tombul@tarim.gov.tr

Kantonales Labor Zürich

Fehrenstrasse 15

8032 Zürich

Switzerland

Attn: René Köppel

Phone: +41 43 244 72 24

rene.koeppel@klzh.ch
83

Kantonales Laboratorium Bern

Muesmalttstrasse 19

CH-3012 Bern

Switzerland

Attn: Dr. Christoph Graf

Phone: +41 31 633 11 56

christoph.graf@gef.be.ch

4976

Laboratory of Bromatology and 

Hydrology

Faculty of Pharmacy, University of Porto

Rua de Jorge Viterbo Ferreira, 228 (Ed. 2, 

P3), 4050-313 Porto

Portugal

Attn: Isabel Mafra

Phone: +351 220428640

isabel.mafra@ff.up.pt

2727

Laboratoire Phyotocontrol

Parc Scientifique G. Besse II

180 Rue Philippe Maupas

30035 Nimes

France

Attn: Marine Zitelli

Phone: 00 33 434147000

marinezitelli@phytocontrol.com

Laboratorio Central de Veterinaria

Ministerio de Agricultura Alimentacion y 

Medio Ambiente

Carretera M106-km14, 28110-Algete

Madrid 28110

Spain

Attn: Maria del Valle Perez Barbachano

Phone: 0034913474561

mperezba@magrama.es

Laboratorio COOP ITALIA

Via del Lavoro 6/8

40033 Casalecchio di Reno

Bologna

Italy

Attn: Raffaella Bergami / Sonia Scaramagli

Phone: 0039-051-596169

raffaella.bergami@coopitalia.coop.it/

sonia.scaramagli@coopitalia.coop.it

Laboratorio De Sanidad Exterior De 

Santander

Calle Antonio López, 3 39009 SantanderCantabria

Spain

Attn: Fernado Del Hierro Vega

Phone: +34942999066

fernandodel.hierro@correo.gob.es

7028

LANAGRO-GO

Ministry of Agriculture, Livestock and 

Food Supply

Rua da Divisa s/n Setor Jaó

Goiánia CEP: 74674-025

Brazil

Attn: Regina M. Sartori Coelho / Maria da 

Glória Trindade

Phone: 55 62 81385701 / 55 62 32327222

regina.sartori@agricultura.gov.br

maria.trindade@agricultura.gov.br

3928

Landesamt fuer Verbraucherschutz 

Sachsen-Anhalt

Freiimfelder Str. 68

06112 Halle

Germany

Attn: Dr. Dietrich Maede

Phone: +49 345 5643 313

dietrich.maede@lav.ms.sachsen-anhalt.de

1870
84

LUFA-ITL GmbH

Dr.-Hell-Str. 6

Kiel, Schleswig-Holstein 24107

Germany

Attn: Daniel Plaul

Phone: 0049 431 1228 420

daniel.plaul@agrolab.de

Microbac Laboratories

800 Oak Ridge Turnpike, Suite A-702

Oak Ridge, TN 37830

USA

Attn: Robert Brooks

Phone: 865-687-4887

robert.brooks@microbac.com

Monsanto Company (Creve Coure)

Mailzone N3NB

800 North Lindbergh

St. Louis, MO 63167

USA

Attn: Melania Harris

Phone: 314-694-7524

melania.l.harris@monsanto.com

Monsanto- Europe

Croix de Pardies

F-40305 Peyrehorade

France

Attn: Bruno Zaccomer

Phone: +33 558 73 21 64

bruno.zaccomer@monsanto.com / 

marjorie.caen@monsanto.com

Nanolab Akademicy and Laboratory 

Services Trade Limited Company

Balikesir asphalt 6. km

Bandirma, Balikesir 10200

Turkey

Attn: Nihan DEMİR

Phone: +90 266 733 90 22/23

nihan@nanolabakademi.com / 

info@nanolabakademi.com

7032

National Center for Molecular 

Characterization of GMOs

Shanghai Jiao Tong University

800 Dongchuan Road

Shanghai 200240

China

Attn: Jianxin Shi

Phone: 0086-21-34207174

jianxin.shi@sjtu.edu.cn

National Collateral Management 

Services Ltd

Testing Services. – Commgrade

Hyderabad, Telangana, 500039

India

Attn: G. Parvathi

Phone: 040-66374742

quality@ncml.com

National Food Reference Laboratory

Fatih Sultan Mehmet Boulevard No:70 

Tarım Kampüsü

Yenimahalle/ ANKARA 06170

Turkey

Attn: Şafak B. KARAKOÇ, PhD

Phone: +90 312 327 41 81/1305

safakkarakoc@gmail.com

7030

NDI ADRL Inc. dba Diteba

1620 Tech Avenue, Unit #3

Mississauga, ON, L4W 5P4

Canada

Attn: Olga Akimova

Phone: 1-800-671-9053

olga.akimova@diteba.com
85

Nippon Yuryo Kentei Kyokai Yokohama 

Laboratory

(Japan Oil Stuff Inspectors Corporation)

Nagataminami1-2-34

Minami-Ku,Yokohama

232-0073, Japan

Attn: Kumi Goto

Phone: 045-722-3605

goto3045@nykk.or.jp

OMIC USA INC

3344 NW Industrial Street

Portland OR 97210

USA

Attn: Chong Singsit

Phone: 503 223 1497

c.singsit@omicusa.com

1761

Plant Technology Laboratories

Agricultural Technology Research Institute

No.1, Ln.51, Dahu Rd.

Xiangshan Dist., Hsinchu City 300

Taiwan

Attn: Kuan-Te Li

Phone: 886-3-5185149

kli@mail.atri.org.tw

PLANTON GmbH

Am Kiel-Kanal 44

D-24106 Kiel

Germany

Attn: Christina Hempel

Phone: +49431380150

hempel@planton.de

Punjab Biotechnology Incubator

SCO, 7 & 8, Phase-V

Mohali 160059

India

Attn: Dr. (Mrs) Ajit Dua

Phone: 172-5020894

pbti2015@yahoo.in

Quality Services International GmbH

Flughafendamm 9a

28199 Bremen

Germany

Attn: Tobias Wiezorek

Attn: 0421-59 66 07-0

tobias.wiezorek@qsi-q3.de / 

ute.burfeind@qsi-q3.de

RAGT Semences

Les Molinières

F-12450 La Primaube

France

Attn: Aurelie Bousquet

Phone: +33 565 712 520

aubousquet@ragt.fr

SENASICA, Centro Nacional de 

Referencia en Detección de Organismos 

Geneticamente Modificados

km 37.5 Carretera Federal México-Pachuca

Tecámac, C.P 55740

Mexico

Attn: Maria Guadalupe Barrera Andrade

Phone: 52 55 59051000 ext 53039

maria.barrere@senasica.gob.mx

SGS Argentina S.A

Tronador 4890 - 3rd Floor - Ciudad 

Autonoma de Buenos Aires

(C1430DNN)

Argentina

Attn: Patricia Sanchez

Phone: (54) 011-4124-2113

ar.calidad@sgs.com

SGS Bulgaria Ltd - Laboratory Varna

1 William Froude Str.

Institute for Hydor and Aerodymanics, 7th

floor

9003 Varna

Bulgaria

Attn: Veselka Pashova

Phone: +359(52)358090

veselka.pashova@sgs.com
86

SGS India Private Limited

201, Sumel II, Nr. Gurudwara

S. G. Highway

Ahmedabad, Gujarat 380 054

India

Attn: Ujjal Roy

Phone: +91-79-61603100

ujjal.roy@sgs.com

SGS North America

1405 32nd Ave.

Brookings, SD 57006

USA

Attn: Heather Waxdahl

Phone: 605-692-7611

heather.waxdahl@sgs.com

Sistémas Genomicos. S. L.

Parque Tecnológico de Valencia

Ronda G. Marconi, 6

46980 Paterna, Valencia

Spain

Attn: Amparo Girós Pérez

Phone: +34 961 366 150

amparo.giros@sistemasgenomicos.com /

calidad@sistemasgenomicos.com

Synlab Umweltinstitut GmbH 

Lebensmittelinstitut Jena Orlaweg 2

Loebstedter Strasse 80

Jena, Thueringen, 00749

Germany

Attn: Dr. Reinhard Baier

Phone: ++493641-3096420

r.baier@food-jena.de

Thionville Laboratories, Inc.

5440 Pepsi St.

New Orleans, LA 70123

USA

Attn: Paul Wacker

Phone: 504-733-9603

labii@thionvillenola.com

Tida Hain India Pvt Ltd

Plot no 3 6th floor, Sector 32

Opp. Tau Devi Lal Sports Complex

Gurgaon 122001, Haryana

India

Attn: Surendra Pal

Phone: 91-9810274739

surender@tilda.com

Tobacco Research Board

Kutsaga Research Station

Airport Ring Rd.

Box 1909, Harare

Zimbabwe

Attn: Fortunate Mufunda

Phone: +263-4-575289/94, +263-4-575412

fmufunda@kutsaga.co.zw

1854

Voivodship Sanitary and 

Epidemiological Station in Bialystok

Genetically Modified Food Analysis Section

ul. Legionowa 8

15-099 Bialystok

Poland

Attn: Grazyna Ostrowska

Phone: +48 508859706

sekretariat@wsse.bialystok.pl