# Patent

## Title
Multipart fluid system and a system for regional citrate anticoagulation in an extracorporeal blood circuit

## Abstract

The present invention concerns a multipart fluid system for use in CRRT, wherein the multipart fluid system comprises an anticoagulation fluid and at least one fluid from the group consisting of a dialysis fluid and an infusion fluid. According to the invention the anticoagulation fluid comprises at least 8 mM citrate, and the dialysis fluid and/or infusion fluid comprises 2-8 mM citrate and 1-5 mM total calcium. The present invention further concerns a system for regional citrate anticoagulation in an extracorporeal blood circuit.

## Background
CROSS RELATED APPLICATIONS
This application is the US national phase of international application PCT/EP2010/054267 filed 31 Mar. 2010 and claims priority to Swedish Patent Application No. 0900422-7 filed 31 Mar. 2009 and U.S. Provisional Application No. 61/164,922 filed 31 Mar. 2009 which designated the U.S. and the entire contents of each of these applications are hereby incorporated by reference.


TECHNICAL FIELD
The present invention concerns a multipart fluid system for use in CRRT. More particularly it relates to an anticoagulation fluid and at least one fluid from the group consisting of a dialysis fluid and an infusion fluid.
The present invention further concerns a system for regional anticoagulation in an extracorporeal blood treatment.
BACKGROUND
Dialysis is the indicated treatment for patients with renal insufficiency. The removal of waste substances and excess of fluid from the blood is effected by transfer to an external fluid or by replacement of plasma liquid with an external fluid. Various dialysis techniques with associated dialysis fluids, may be differentiated. Which dialysis technique to use, depend on the type of patient.
In the case of patients suffering from acute renal insufficiency, a continuous treatment throughout the entire day for several weeks, a continuous renal replacement therapy (CRRT), is the indicated treatment.
Continuous renal replacement therapy (CRRT) is also the treatment mode when a patient with chronic renal insufficiency is using a wearable artificial kidney system, such a system is for example disclosed in US 2008/058696.
In a CRRT treatment a portion of the patient's blood stream is lead into an extracorporeal blood circuit comprising a semipermeable membrane in which the removal of waste substances and excess fluid is performed and then the cleansed blood is lead back to the patient. The semipermeable membrane has a blood side and a dialysate side.
When the removal of waste substances and excess fluid is effected by transfer to an external fluid, the waste substances and excess fluid are transferred by diffusion through the semipermeable membrane wall into a dialysis fluid flowing on the dialysate side of the semipermeable membrane. This technique is called hemodialysis.
When the removal of waste substances and excess fluid is made by replacement of plasma liquid with an external fluid, a portion of the plasma liquid is removed from the blood by means of convective flow through the semipermeable membrane, and an external fluid (also called a replacement fluid or an infusion fluid) is added to the blood stream. This technique is called hemofiltration.
Finally the removal of waste substances and excess fluid may also be made by a combination of hemodialysis and hemofiltration, thus the removal of waste substances and excess fluid is provided by a combination of diffusion and convection through the semipermeable membrane. This technique is called hemodiafiltration.
Common for all the above disclosed techniques is that the blood is withdrawn from the patient continuously into an extracorporeal blood circuit, in which the removal takes place, and the “cleansed” blood is returned to the patient. When blood is removed from its normal environment within the blood vessels, the blood coagulation cascade is initiated, and in order not to clog the extracorporeal blood circuit with the coagulating blood, means for anticoagulation have to be provided.
The use of citrate as an anticoagulant for intensive care patients is increasing. A fluid containing sodium citrate and/or citric acid is then infused close to the blood access where the blood exits the patient and enters into the extracorporeal blood circuit. Citrate acts as an anticoagulant by lowering the ionized calcium concentration within the plasma, through calcium citrate complex formation. Ionized calcium is essential for the blood coagulation cascade. If the ionized calcium level is lowered well below 0.5 mM, the blood coagulation cascade is prevented. Citrate that exists in the blood is rapidly metabolized in the liver, forming three bicarbonate ions per citrate ion. As the citrate concentration is lowered in the metabolism, citrate complex bound calcium is released and returns to ionized calcium.
Today the use of citrate as anticoagulant is usually combined with the use of a dialysis fluid or an infusion fluid not containing any calcium, which means that a significant amount of calcium will be removed in the semipermeable membrane. This calcium has to be replaced in order not to create dangerously low levels of ionized calcium in the blood of the patient. The replacement of removed calcium is usually done by direct infusion of a fairly concentrated calcium fluid, either calcium chloride or, in increasing number of cases, calcium gluconate. This infusion may be done either into the line set for the extracorporeal blood circuit, close to the blood return to the patient, or directly into the vein of the patient.
The balancing of the correct amount of calcium within the blood in order to replace the removal in the semipermeable membrane is a delicate matter. Both too much and too little calcium within the blood may lead to serious patient injury, and if not handled correctly it is potentially fatal to the patient. A close supervision of the patient's ionized calcium level is therefore necessary. Many attempts have therefore been made to avoid the calcium infusion by having calcium in the dialysis and/or infusion fluids. However, it is difficult to have enough calcium in these fluids without causing clotting problems.
SUMMARY OF THE INVENTION
One object of the present invention is to provide a multipart fluid system for citrate anticoagulation with which the removal of calcium within the dialysis process is reduced or prevented without increasing the risk of clotting.
The present invention concerns a multipart fluid system for use in CRRT, wherein the multipart fluid system comprises an anticoagulation fluid and at least one fluid from the group consisting of a dialysis fluid and an infusion fluid. According to the invention the anticoagulation fluid comprises at least 8 mM citrate, and the dialysis fluid and/or infusion fluid comprises 2-8 mM citrate and 1-5 mM total calcium.
In one embodiment of the present invention said anticoagulation fluid further comprises 1.5-4 mM total calcium. In another embodiment said anticoagulation fluid comprises 2-3 mM total calcium. In a further embodiment said anticoagulation fluid comprises 2.2-2.6 mM total calcium. In another embodiment said anticoagulation fluid comprises about 2.4 mM total calcium.
In another embodiment of the present invention said anticoagulation fluid comprises 8-50 mM citrate. In a further embodiment said anticoagulcation fluid comprises 10-50 mM citrate. In even a further embodiment said anticoagulation fluid comprises 17-50 mM citrate. In a further embodiment said anticoagulation fluid comprises 18-50 mM citrate.
In even another embodiment of the present invention said anticoagulation fluid further comprises 0-1.5 mM magnesium, 0-5.0 mM potassium, 0-11 mM glucose, 130-150 mM sodium and 0-140 mM chloride.
In one embodiment of the present invention the dialysis fluid and/or infusion fluid comprises 2.0-7.0 mM citrate and 1.8-2.4 mM total calcium. In another embodiment the dialysis fluid and/or influsion fluid comprises 3.0-5.0 mM citrate and 1.8-2.4 mM total calcium.
In even another embodiment of the present invention, the dialysis and/or infusion fluid further comprises 0-1.5 mM magnesium, 0-5.0 mM potassium, 0-11 mM glucose, 130-150 mM sodium and 80-150 mM chloride, and 0-2.8 mM phosphate.
In another embodiment of the present invention, the dialysis and/or infusion fluid further comprises a physiological buffer. In one embodiment said physiological buffer is bicarbonate. In another embodiment the dialysis fluid and/or infusion fluid comprises <25 mM bicarbonate.
In yet another embodiment said dialysis and/or infusion fluid further comprises 0.1-3.0 mM gluconic acid.
In another embodiment said dialysis and/or infusion fluid further comprises 0.5-2.9 mM gluconic acid.
In even a further embodiment said infusion fluid is to be used as post infusion fluid.
In another embodiment said multipart fluid system only comprises an anticoagulation fluid and a dialysis fluid according to above embodiments.
In another embodiment said multipart fluid system only comprises an anticoagulation fluid and at least one infusion fluid according to above embodiments.
In another embodiment said multipart fluid system only comprises an anticoagulation fluid, a dialysis fluid and at least one infusion fluid according to above embodiments.
In another embodiment said multipart fluid system does not comprise a post infusion fluid comprising >6 mM total calcium.
The present invention further concerns a system for regional citrate anticoagulation in an extracorporeal blood circuit comprising an arterial blood line configured to be connected to a vascular access for withdrawing blood from a patient and a venous blood line configured to be connected to the vascular access for returning blood to the patient. According to the present invention this system comprises a filter (a semipermeable membrane) with a dialysate side and a blood side, which blood side is in fluid communication with the arterial and venous blood lines; a pre-filter infusion line connected to the arterial blood line upstream the filter and connected to an anticoagulation fluid source containing at least 8 mM citrate to be infused into the blood stream in the arterial blood line; and a post-filter infusion line connected to the venous blood line downstream the filter and connected to an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium to be infused into the blood stream in the venous blood line. Thus, the pre-filter infusion line is in fluid communication with an anticoagulation fluid source containing at least 8 mM citrate, and the post-filter infusion line is in fluid communication with an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium.
In another embodiment of this system the dialysate side of the filter is in fluid communication with a dialysis fluid source comprising 2-8 mM citrate and 1-5 mM total calcium.
In even another embodiment of this system, this system does not comprise any post-filter infusion line being in fluid communication with any infusion fluid sources comprising >6 mM total calcium. A post-filter infusion line being an infusion line connected to the venous blood line downstream the filter. Thus, no post-filter infusion line is connected to an infusion fluid source comprising >6 mM total calcium.
The present invention further concerns a system for regional citrate anticoagulation in an extracorporeal blood circuit comprising an arterial blood line configured to be connected to a vascular access for withdrawing blood from a patient and a venous blood line configured to be connected to the vascular access for returning blood to the patient. According to the present invention this system comprises a filter with a dialysate side and a blood side, which blood side is in fluid communication with the arterial and venous blood lines, and which dialysate side is in fluid communication with a dialysis fluid source comprising 2-8 mM citrate and 1-5 mM total calcium; and a pre-filter infusion line connected to the arterial blood line upstream the filter and connected to an anticoagulation fluid source containing at least 8 mM citrate to be infused into the blood stream in the arterial blood line.
In another embodiment of this system, this system does not comprise any post-filter infusion line being connected to any infusion fluid sources comprising >6 mM total calcium. Thus, the pre-filter infusion line is in fluid communication with an anticoagulation fluid source containing at least 8 mM citrate, and no post-filter infusion line is connected to any infusion fluid sources comprising >6 mM total calcium.
The present invention further concerns a system for regional citrate anticoagulation in an extracorporeal blood circuit comprising an arterial blood line configured to be connected to a vascular access for withdrawing blood from a patient and a venous blood line configured to be connected to the vascular access for returning blood to the patient. According to the present invention this system comprises a filter with a dialysate side and a blood side, which blood side is in fluid communication with the arterial and venous blood lines; a first pre-filter infusion line connected to the arterial blood line upstream the filter and connected to an anticoagulation fluid source containing at least 8 mM citrate to be infused into the blood stream in the arterial blood line; and a second pre-filter infusion line connected to the arterial blood line upstream the filter and connected to an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium to be infused into the blood stream in the arterial blood line. Thus, the first pre-filter infusion line is in fluid communication with an anticoagulation fluid containing at least 8 mM citrate, and the second pre-filter infusion line is in fluid communication with an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium.
In another embodiment this system further comprises a post-filter infusion line connected to the venous blood line downstream the filter and connected to an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium to be infused into the blood stream in the venous blood line. Thus, the post-infusion line is in fluid communication with an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium.
In yet another embodiment of this system the dialysate side of the filter is in fluid communication with a dialysis fluid comprising 2-8 mM citrate and 1-5 mM total calcium.
In even another embodiment of this system, this system does not comprise any post-filter infusion line being connected to any infusion fluid sources comprising >6 mM total calcium.
In other embodiments of the systems according to the present invention, said anticoagulation fluid further comprises 1.5-4 mM total calcium.
Yet another embodiment of the systems according to the present invention further comprises a control unit configured to control the anticoagulation fluid flow rate in relation to the blood flow rate. By having such a control unit, the system is monitoring and securing that the amount of citrate within the blood is enough to avoid coagulation within the extracorporeal blood circuit.
With the multipart fluid systems according to the present invention and the systems for regional citrate anticoagulation in an extracorporeal blood circuit according to the present invention it has surprisingly been shown that a separate calcium infusion may be avoided.



BRIEF DESCRIPTION OF DRAWINGS
FIG. 1-8 shows different embodiments of the system for regional citrate anticoagulation in an extracorporeal blood circuit.



DEFINITIONS
The term “CRRT” means a continuous renal replacement therapy and this type of treatment mode is used in case of acute renal insufficiency or in case of chronic renal insufficiency when using a wearable artificial kidney system.
The term “anticoagulation fluid” means a fluid which is intended to provide for the anticoagulation effect within the extracorporeal blood circuit and which is intended to be infused within the extracorporeal blood circuit.
The term “dialysis fluid” means a fluid for perfusion of a filter (also called a semipermeable membrane, a dialyzer, a dialysis filter or a dialysis membrane) on the dialysate side of such a filter.
The term “infusion fluid” means a fluid which is infused into the extracorporeal blood circuit either for predilution, i.e. infused into the extracorporeal blood flow before the blood enters the dialyzer, or for postdilution, i.e. infused into the extracorporeal blood flow after the blood has exited the dialyzer and before the blood is returned to the patient. Infusion fluids is normally also named as replacement fluids, substitution fluids or hemofiltration fluids.
The term “total calcium concentration” means the total amount of calcium present in a fluid, thus representing the sum of calcium present as ionized, complex bound and protein bound calcium.
The term “gluconic acid” means that the component may be added as gluconic acid, glucono-δ-lactone or as its sodium, calcium, magnesium or potassium salt thereof, i.e. gluconate, to the ready-for-use dialysis solution.
The term “citric acid” means that the component may be added as citric acid or as its sodium, magnesium or potassium salt thereof, i.e. citrate, to the ready-for-use dialysis solution.
DETAILED DESCRIPTION OF THE INVENTION
New treatment modalities have been suggested and have been evaluated by use of an in-house software application CitRRT (Rada 20051). The program computes equilibrium concentrations of species present in plasma and fluids used for dialysis by use of equilibrium constants (SCD base 20012). The species (electrolytes, albumin and formed complexes) taken into account are shown in Table 1.







TABLE 1







Normal plasma concentration of electrolytes and albumin together with


the complexes taken into account formed with Ca2+ and Mg2+










Normal plasma




total conc
Complex formation


Specie
(mM) (Kratz 19983)
with Ca2+ and Mg2+





Ca2+
2.1-2.6




(1.1-1.3 ionized)


Mg2+
0.7-1.0


Na+
135-145


Cl−
100-108


HCO3−, bicarbonate, bic
22-26
CaHCO3+, MgHCO3+


C6H5O73−, citrate, cit
0.1-0.3
Ca-cit−, Ca-cit24−,



(infants, Ames19504)
Mg-cit−. Mg-cit24−


Albumin, alb
0.5-0.6
Alb-Can, alb-Mgn,




n = 1-10









The requirements of the treatments are:

    
    
        1. An adequate dialysis treatment
        2. A sufficient anticoagulant effect, i.e. the ionized calcium through the blood side of the filter (dialyzer) must be 0.2-0.5 mM, preferably 0.3-0.4 (according to literature)
        3. The total plasma concentration of calcium when returned to the patient has to be at normal level, i.e. about 2.5 mM. Other electrolyte concentration levels must also be satisfactory.
    
    


Four different treatment modalities were evaluated, also shown in Table 2, and these are:

    
    
        1. As common today, with citrate and NaCl in the anticoagulation fluid, all normal ions except Ca2+ in the dialysis fluid, and infusion of Ca2+ from a calcium syringe.
        2. Citrate and NaCl in the anticoagulation fluid, all normal ions including Ca2+ in the dialysis fluid. To investigate if additional Ca2+ from a calcium syringe may be avoided.
        3. Citrate and NaCl in the anticoagulation fluid, citrate plus all normal ions including Ca2+ in the dialysis fluid. To investigate if additional Ca2+ from a calcium syringe may be avoided.
        4. Citrate plus all normal ions including Ca2+ in the anticoagulation fluid, citrate plus all normal ions in the dialysis fluid. To investigate if additional Ca2+ from a calcium syringe may be avoided.
    
    









TABLE 2







The evaluated treatment modalities:













Calcium



Anticoagulation

infusion


Modality
fluid
Dialysis fluid
mandatory?





1.
Cit3−, Na+, Cl−
Na+, Mg2+, K+, HCO3−,
Yes




Cl−


2.
Cit3−, Na+, Cl−
Ca2+, Na+, Mg2+, K+,
?




HCO3−, Cl−


3.
Cit3−, Na+, Cl−
Cit3−, Ca2+, Na+, Mg2+,
?




K+, HCO3−, Cl−


4.
Cit3−, Ca2+, Na+,
Cit3−, Ca2+, Na+, Mg2+,
?



Mg2+, K+, HCO3−,
K+, HCO3−, Cl−



Cl−









Reasonable flow rates of blood (125 mL/min) and dialysis fluid (2500 mL/h) (varied within reason), normal steady state concentrations of calcium (2.5 mM) and magnesium (0.9 mM), and suitable concentrations of ions in the anticoagulation fluid and dialysis fluid (varied) are assumed as well as a hematocrit (Hct) of 30%, an albumin plasma concentration of 40 g/L, and a pCO2 of 40 mmHg. A disposable set with a dialyzer with a KoA for urea of 420 is assumed. This is sufficient for clearance to be determined only by the fluid flow rates.
Calculations were done using the following standard fluids (only or as a base); dialysis fluid PrismaSate® B22GK4/0 (PS) (0.75 mM Mg2+, 140 mM Na+, 4 mM K+, 120.5 mM Cl−, 3 mM lactate, 22 mM HCO3−, 110 mM glucose); anticoagulation fluid Prismocitrate® 10/2 (PC) (12 mM citrate (10 mM from Na3cit and 2 mM form H3cit), 136 mM Na+, 106 mM Cl−); and anticoagulation fluid ACD-A (113 mM citrate (75 mM from Na3cit and 38 mM form H3cit), 225 mM Na+, 136 mM D-glucose). The calcium infusion fluid for the calcium syringe contained 225 mM calcium gluconate.
Results
Modality 1
Examples are shown in Table 3.







TABLE 3







Modality 1. Examples when using the dialysis fluid PrismaSate ® B22GK4/0 (PS) and


combining it with the anticoagulation fluid Prismocitrate ® 10/2 (PC) or


the anticoagulation fluid ACD-A.










Into dialyzer
After dialyzer
















Total
Ionized
Total
Total
Ionized
Infused

Total


Fluids
Ca2+
Ca2+
citrate
Ca2+
Ca2+
Ca2+
Clearance
citrate





PS
1.9
0.40
3.1
1.3
0.36
6.2 mM/h
2955 mL/h
2.0


PC


PS
2.4
0.40
4.3
1.4
0.36
5.7 mM/h
2536 mL/h
2.3


ACD-A









Since the anticoagulation fluid ACD-A fluid is much more concentrated than the dialysis fluid PrismaSate®, the blood flow is less diluted, and therefore the total calcium concentration into the dialyzer is higher. As a consequence the total citrate into the patient is a bit higher in this case and the need for infused calcium is slightly lower. The bicarbonate concentration into the patient is a bit higher. Since the flow rate into the dialyzer is lower, the clearance of the treatment becomes lower.
Modality 2
In this case, calcium is added to the standard dialysis fluid. The blood flow rate is set to 125 ml/min and the citrate and calcium concentrations and the flow rate of the dialysis fluid may be varied. In order to have any chance of receiving correct (≦0.4 mM) ionized calcium as well as correct total calcium (2.5 mM) without infusion of calcium fluid, the ionized calcium concentration into the dialyzer was set to different values between 0.25 and 0.4 mM. It is not possible to obtain high enough plasma total calcium concentration out of the dialyzer (into the patient) with a ionized calcium concentration (≦0.4 mM, no matter how the dialysis fluid flow rate and the calcium concentration in the dialysis fluid are changed. Examples are shown in Table 4. In the case with the anticoagulation fluid ACD-A, the bicarbonate concentration was too high, i.e. the bicarbonate concentration must be lower than 22 mM in the dialysis fluid (which is the concentration in the dialysis fluid PrismaSate® B22GK4/0).







TABLE 4







Modality 2. Examples when using the dialysis fluid PrismaSate ® B22GK4/0 (PS) and


adding Ca2+ to the dialysis fluid, and combining it with the anticoagulation fluid


Prismocitrate ® 10/2 (PC) or the anticoagulation fluid ACD-A.










Into dialyzer
After dialyzer
















Total
Ionized
Total
Total
Ionized
Infused

Total


Fluids
Ca2+
Ca2+
citrate
Ca2+
Ca2+
Ca2+
Clearance
citrate





PS + Ca2+,
1.7
0.25
4.0
(2.1)
0.4
—
(2544)
(3.4) not


PC







sufficient










effect


PS + Ca2+,
2.4
0.25
6.0
(2.0)
0.4
—
(2556)
(3.3) not


ACD-A







sufficient










effect









This modality does not seem to work neither theoretically, nor clinically. In clinical tests, it has not been possible to perform this kind of dialysis without need for infusion of calcium or clotting of the patients' blood (Gupta 20045, Cointault 20046).
Modality 3
In this case, calcium and citrate are added to the modified standard dialysis fluid. The concentrations of the included electrolytes were changed until correct values out of the dialyzer were achieved (alternatively, the dialysis fluid flow rate was changed). When using the anticoagulation fluid Prismocitrate® 10/12 (PC), the concentrations in the dialysis fluid may be: 3.35 mM Ca2+, 7.0 mM citrate, 1.2 mM Mg2+, 142 mM Na+, 5.9 mM K+, 107 mM Cl−, 29 mM bicarbonate, and the dialysis fluid flow 2500 mL/h (see results in Table 5). When using the anticoagulation fluid ACD-A, the concentrations in the dialysis fluid may be: 2.4 mM Ca2+, 4.8 mM citrate, 0.9 mM Mg2+, 136 mM Na+, 4.2 mM K+, 112 mM Cl−, 20 mM bicarbonate, and the dialysis fluid flow 2500 mL/h (see results in Table 5).







TABLE 5







Modality 3. Examples when using the dialysis fluid PrismaSate ® B22GK4/0 (PS) and


adding Ca2+ and citrate to the dialysis fluid and combining it with the anticoagulation


fluid Prismocitrate ® 10/2 (PC) or the anticoagulation fluid ACD-A.










Into dialyzer
After dialyzer
















Total
Ionized
Total
Total
Ionized
Infused

Total


Fluids
Ca2+
Ca2+
citrate
Ca2+
Ca2+
Ca2+
Clearance
citrate





PS + Ca2+
1.9
0.40
3.1
2.5
0.40
—
2962
4.5


and cit PC


PS + Ca2+
2.4
0.40
4.3
2.5
0.40
—
2538
4.5


and cit ACD-A









For the anticoagulation fluid Prismocitrate® 10/12, if the dialysis fluid flow rate is changed to 2000 (or 3000) mL/h, then the concentrations out of the dialyzer are slightly affected (compared with concentrations in table 5 above); e.g. total Ca2+2.4 (2.6) mM, total Mg2+0.8 (0.9) mM and total K+3.6 (4.4), and the clearances become 2666 (3253) mL/h. When changing the dialysis fluid flow rate in the example with the anticoagulation fluid ACD-A, the concentrations are affected as well but to a smaller extent.
Modality 4
In this case, the goal was to keep the concentrations of the included electrolytes constant through the dialyzer. When possible, both anticoagulation fluid and dialysis fluid contained normal total plasma values of Ca2+, Na+, K+, Mg2+ and Cl−. However, the Ca2+ and Mg2+ concentrations in the dialysis fluid were slightly lower than normal due to that the plasma flow through the dialyzer needs to be concentrated since it has become diluted by the anticoagulation fluid. Different citrate concentrations in the anticoagulation fluids were investigated, and the concentration of Cl− in this fluid depends on the chosen citrate concentration. The concentration of citrate in the dialysis fluid was chosen in order to keep the ionized calcium concentration constant through the dialyzer.
Three different anticoagulation fluids were investigated, one based on the anticoagulation fluid Prismocitrate® 10/12, one on the anticoagulation fluid ACD-A, and one anticoagulation fluid with a citrate concentration in between.
Examples are shown in Table 6.
Alternative 1: To the anticoagulation fluid Prismocitrate® 10/12 was added 2.5 mM Ca2+, 0.9 mM Mg2+ and 4.0 mM K+. In combination therewith, the dialysis fluid contained 2.1 mM Ca2+, 4.1 mM citrate, 0.8 mM Mg2+, 144 mM Na+, 4.0 mM K+, 122 mM Cl−, 20 mM bicarbonate with a dialysis fluid flow of 2500 mL/h.
Alternative 2: Citrate in a fluid may come from Na3cit or H3cit or a combination. In order to receive as high pH as possible (about 6.4), i.e. as close to the normal plasma pH of 7.4 as possible, all citrate should come from Na3cit. The desired Na+ concentration in the anticoagulation fluid is 140 mM and the citrate concentration in this case would be 140/3=67.6 mM. 2.5 mM Ca2+, 0.9 mM Mg2+ and 4.0 mM K+ was added to this anticoagulation fluid. In combination therewith, the dialysis fluid contained 2.25 mM Ca2+, 4.5 mM citrate, 0.85 mM Mg2+, 140 mM Na+, 4.0 mM K+, 122 mM Cl−, 15 mM bicarbonate with a dialysis fluid flow of 2500 mL/h.
Alternative 3: To the anticoagulation fluid ACD-A was added 2.5 mM Ca2+, 0.9 mM Mg2+ and 4.0 mM K+. In combination therewith, the dialysis fluid contained 2.25 mM Ca2+, 4.5 mM citrate, 0.85 mM Mg2+, 140 mM Na+, 4.0 mM K+, 122 mM Cl−, 15 mM bicarbonate with a dialysis fluid flow of 2500 mL/h.







TABLE 6







Modality 4. Examples when using the dialysis fluid PrismaSate ® B22GK4/0 (PS) and


adding Ca2+ and citrate to the dialysis fluid and combining it with three different 


anticoagulation fluids based on the anticoagulation fluid Prismocitrate ® 10/12, the


anticoagulation fluid ACD-A or one anticoagulation fluid with a citrate concentration in


between.










Into dialyzer
After dialyzer
















Total
Ionized
Total
Total
Ionized
Infused

Total


Fluids
Ca2+
Ca2+
citrate
Ca2+
Ca2+
Ca2+
Clearance
citrate





PS + Ca2+ and
2.5
0.40
4.8
2.5
0.40
—
3409
4.6


cit


PC 10/2 + Ca2+


PS + Ca2+ and
2.5
0.40
4.6
2.5
0.40
—
2622
4.5


cit


67.6 citrate +


Ca2+


PS + Ca2+ and
2.5
0.40
4.5
2.5
0.40
—
2539
4.5


cit


ACD-A + Ca2+









The flow rates of dialysis fluid (1000-4500 mL/h, with a constant blood flow rate of 125 mL/min) and blood (50-200 mL/min, with a constant dialysis fluid rate of 2500 mL/h) may be widely varied without any significant influence on the electrolyte concentrations, except bicarbonate.
Evaluation
Modality 1 is the treatment technique used today when performing citrate dialysis. It would be desirable to avoid the calcium infusion that is necessary for this treatment. This cannot be achieved with modality 2 but with 3 and 4. Modality 3 and 4 are comparable regarding the total citrate concentration the patient is subjected to, but modality 4 is much more insensitive to changes in the flow rates of blood, dialysis fluid and anticoagulation fluid since the system is closer to equilibrium through the dialyzer in modality 4.
DETAILED DESCRIPTION OF THE DRAWINGS
In FIG. 1 is shown a system for regional citrate anticoagulation in an extracorporeal blood circuit comprising an arterial blood line 1 configured to be connected to a vascular access (not shown) for withdrawing blood from a patient and a venous blood line 2 configured to be connected to the vascular access (not shown) for returning blood to the patient. This system comprises a filter 3 with a dialysate side and a blood side, which blood side is in fluid communication with the arterial blood line 1 and venous blood line 2; a pre-filter infusion line 4 connected to the arterial blood line 2 upstream the filter 3 for infusing an anticoagulation fluid 5 containing citrate into blood in the arterial blood line 1; and a post-filter infusion line 6 connected to the venous blood line 2 downstream the filter 3 for infusing an infusion fluid 7 comprising 2-8 mM citrate and 1-5 mM total calcium into blood in the venous blood line 2. An effluent bag 8 is provided in fluid communication with the dialysate side of the filter 3, to collect the plasma water (ultrafiltrate) withdrawn from the blood passing the filter 3.
In FIG. 2 is shown another embodiment of the system in FIG. 1 with the addition that the dialysate side of the filter 3 is in fluid communication with a dialysis fluid 9 comprising 2-8 mM citrate and 1-5 mM total calcium.
In FIG. 3 is shown another system for regional citrate anticoagulation in an extracorporeal blood circuit. This system includes an arterial blood line 1 configured to be connected to a vascular access for withdrawing blood from a patient and a venous blood line 2 configured to be connected to the vascular access for returning blood to the patient. This system also comprises a filter 3 with a dialysate side and a blood side, which blood side is in fluid communication with the arterial and venous blood lines, and which dialysate side is in fluid communication with a dialysis fluid 9 comprising 2-8 mM citrate and 1-5 mM total calcium and an effluent bag 8 for the spent dialysis fluid and the plasma water (ultrafiltrate) withdrawn from the blood passing the filter 3. The system further comprises a pre-filter infusion line 4 connected to the arterial blood line 1 upstream the filter 3 for infusing an anticoagulation fluid 5 containing citrate into the blood in the arterial blood line.
In FIG. 4 is shown yet another embodiment of the system for regional citrate anticoagulation in an extracorporeal blood circuit according to the present invention. This system includes an arterial blood line 1 configured to be connected to a vascular access for withdrawing blood from a patient and a venous blood line 2 configured to be connected to the vascular access for returning blood to the patient. This system further comprises a filter 3 with a dialysate side and a blood side, which blood side is configured in fluid communication with the arterial and venous blood lines. A pre-filter infusion line 4 is connected to the arterial blood line 1 upstream the filter 3 for infusing an anticoagulation fluid 5 containing citrate into blood in the arterial blood line 1. A second pre-filter infusion line 10 is connected to the arterial blood line 1 upstream the filter 3 for infusing an infusion fluid 11 comprising 2-8 mM citrate and 1-5 mM total calcium into blood in the arterial blood line 1. Also here an effluent bag 8 is provided in fluid communication with the dialysate side of the filter 3, for receiving the plasma water (ultrafiltrate) withdrawn from the blood passing the filter 3.
In FIG. 5 is another embodiment of the system in FIG. 4 shown, which further comprises a post-filter infusion line 6 connected to the venous blood line 2 downstream the filter 3 for infusing an infusion fluid 7 comprising 2-8 mM citrate and 1-5 mM total calcium into blood in the venous blood line 2.
In FIG. 6 is yet another embodiment of the system in FIG. 4 shown, wherein the dialysate side of the filter 3 is in fluid communication with a dialysis fluid 9 comprising 2-8 mM citrate and 1-5 mM total calcium.
In FIG. 7 is another embodiment of the system in FIG. 6 shown. This system further comprises a post-filter infusion line 6 connected to the venous blood line 2 downstream the filter 3 for infusing an infusion fluid 7 comprising 2-8 mM citrate and 1-5 mM total calcium into the blood in the venous blood line 2.
In FIG. 8 is another embodiment of the systems above shown, which further comprises a control unit 12 adapted to control the anticoagulation fluid flow rate in relation to the blood flow rate. Such a control unit may be provided in all the systems shown in the different embodiments of FIG. 1-FIG. 7. By having such a control unit 12, the system is monitoring and securing that the amount of citrate within the blood is enough to maintain anticoagulation within the extracorporeal blood circuit.
As is evident from all the figures above, the systems do not comprise any post-filter infusion line connected to the venous blood line 2 downstream the filter 3 for infusion of a fluid comprising >6 mM total calcium.
In the systems according to the invention, pumps are configured to pump blood (pump 13) through the extracorporeal blood circuit, anticoagulation fluid (pump 14) into the extracorporeal blood circuit, infusion fluid (pumps 15a and 15b) into the extracorporeal blood circuit, dialysis fluid (pump 16) into the dialysate side of the filter 3, and plasma liquid (ultrafiltrate) and optional spent dialysis fluid (pump 17) out from the dialysate side of the filter 3 and into the effluent bag 8.
It should be understood that various changes and modifications to the presently preferred embodiments described herein will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the spirit and scope of the present invention and without diminishing its attendant advantages. It is therefore intended that such changes and modifications be covered by the appended claims.
REFERENCES


    1Rada H; CitRRT (Internal report, GAMBRO)
    2SCDbase, Stability Constants Database IUPAC, Academic Software, 2001
    3Kratz A et al.; New Engl J Med 339:1063-1067, 1998
    4Ames R et al.; Pediatrics 6:361-370, 1950
    5Gupta M et al.; Am J Kidney Dis 43:67-73, 2004
    6Cointault O et al.; Nephrol Dial Transplant 19: 171-178, 2004

## Claims

1. A multipart fluid system for use in continuous renal replacement therapy (CRRT), said multipart fluid system comprising:
a first vessel configured to be in fluid communication with an extracorporeal blood passage of a CRRT blood treatment apparatus;
a second vessel configured to be in fluid communication with the extracorporeal blood passage;
an anticoagulation fluid in the first vessel and including at least 8 mM citrate, and
at least one fluid in the second vessel, wherein the at least one fluid is from a group consisting of a dialysis fluid and an infusion fluid, and said at least one fluid comprises 2-8 mM citrate and 1-5 mM total calcium.

2. The multipart fluid system according to claim 1, wherein said anticoagulation fluid further comprises 1.5-4 mM total calcium.

3. The multipart fluid system according to claim 1, wherein said anticoagulation fluid comprises 8-50 mM citrate.

4. The multipart fluid system according to claim 1, wherein said anticoagulation fluid comprises 10-50 mM citrate.

5. The multipart fluid system according to claim 1, wherein said anticoagulation fluid comprises 17-50 mM citrate.

6. The multipart fluid system according to claim 1, wherein said anticoagulation fluid comprises 2-3 mM total calcium.

7. The multipart fluid system according to claim 1, wherein said anticoagulation fluid comprises 2.2-2.6 mM total calcium.

8. The multipart fluid system according to claim 1, wherein said anticoagulation fluid further comprises 0-1.5 mM magnesium, 0-5.0 mM potassium, 0-11 mM glucose, 130-150 mM sodium and 0-140 mM chloride.

9. The multipart fluid system according to claim 1, wherein said at least one fluid comprises 2.0-7.0 mM citrate and 1.8-2.4 mM total calcium.

10. The multipart fluid system according to claim 1, wherein said at least one fluid comprises 3.0-5.0 mM citrate and 1.8-2.4 mM total calcium.

11. The multipart fluid system according to claim 1, wherein said at least one fluid further comprises 0-1.5 mM magnesium, 0-5.0 mM potassium, 0-11 mM glucose, 130-150 mM sodium and 80-150 mM chloride, and 0-2.8 mM phosphate.

12. The multipart fluid system according to claim 1, wherein said at least one fluid further comprises a physiological buffer.

13. The multipart fluid system according to claim 12, wherein said physiological buffer is bicarbonate.

14. The multipart fluid system according to claim 13, wherein said at least one fluid comprises no more than 3.1 mM bicarbonate.

15. The multipart fluid system according to claim 9, wherein said at least one fluid further comprises 0.1-3.0 mM gluconic acid.

16. The multipart fluid system according to claim 1, wherein said at least one fluid includes the infusion fluid which is to be used as post infusion fluid.

17. The multipart fluid system according to claim 1, which system does not comprise a post infusion fluid comprising at least 6 mM total calcium.

18. A system for regional citrate anticoagulation in an extracorporeal blood circuit for a continuous renal replacement therapy (CRRT) apparatus, the system including an arterial blood line configured to be connected to a vascular access device for withdrawing blood from a patient and a venous blood line configured to be connected to the vascular access for returning blood to the patient, the system comprising:
a filter in the CRRT apparatus, the filter including a dialysate side and a blood side, which blood side is in fluid communication with the arterial and venous blood lines;
a pre-filter infusion line in the CRRT apparatus and connected to the arterial blood line upstream the filter and connected to an anticoagulation fluid source containing at least 8 mM citrate to be infused into the blood stream in the arterial blood line; and
a post-filter infusion line in the CRRT apparatus and connected to the venous blood line downstream the filter and connected to an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium to be infused into the blood stream in the venous blood line.

19. A system for regional citrate anticoagulation in an extracorporeal blood circuit for a continuous renal replacement therapy (CRRT) apparatus, the system comprising an arterial blood line configured to be connected to a vascular access for withdrawing blood from a patient and a venous blood line configured to be connected to the vascular access for returning blood to the patient, the system comprising:
a filter for the CRRT apparatus, the filter including a dialysate side and a blood side, which blood side is in fluid communication with the arterial and venous blood lines, and which dialysate side is in fluid communication with a dialysis fluid source comprising 2-8 mM citrate and 1-5 mM total calcium; and
a pre-filter infusion line for the CRRT apparatus and connected to the arterial blood line upstream the filter and connected to an anticoagulation fluid source containing at least 8 mM citrate to be infused into the blood stream in the arterial blood line.

20. A system for regional citrate anticoagulation in an extracorporeal blood circuit for continuous renal replacement therapy (CRRT), the system comprising an arterial blood line configured to be connected to a vascular access for withdrawing blood from a patient and a venous blood line configured to be connected to the vascular access for returning blood to the patient, the system comprising:
a filter for a CRRT blood treatment apparatus, the filter including a dialysate side and a blood side, which blood side is in fluid communication with the arterial and venous blood lines;
a first pre-filter infusion line for the CRRT blood treatment apparatus and connected to the arterial blood line upstream the filter and connected to an anticoagulation fluid source containing at least 8 mM citrate to be infused into the blood stream in the arterial blood line; and
a second pre-filter infusion line for the CRRT blood treatment apparatus and connected to the arterial blood line upstream the filter and connected to an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium to be infused into the blood stream in the arterial blood line.

21. A system for regional citrate anticoagulation in an extracorporeal blood circuit according to claim 20, which further comprises a post-filter infusion line connected to the venous blood line downstream the filter and connected to an infusion fluid source comprising 2-8 mM citrate and 1-5 mM total calcium to be infused into the blood stream in the venous blood line.

22. A system for regional citrate anticoagulation in an extracorporeal blood circuit according to claim 18, wherein the dialysate side of the filter is in fluid communication with a dialysis fluid source comprising 2-8 mM citrate and 1-5 mM total calcium.

23. A system for regional citrate anticoagulation in an extracorporeal blood circuit according to claim 18, not comprising any post-filter infusion line connected to any infusion fluid sources comprising at least 6 mM total calcium.

24. A system for regional citrate anticoagulation in an extracorporeal blood circuit according to claim 18, wherein said anticoagulation fluid further comprises 1.5-4 mM total calcium.

25. A system for regional citrate anticoagulation in an extracorporeal blood circuit according to claim 18, wherein said system further comprises a control unit adapted to control the anticoagulation fluid flow rate in relation to the blood flow rate.

26. A method of performing regional citrate anticoagulant dialysis of a patient's blood during continuous renal replacement therapy (CRRT), the method comprising:
a) flowing blood from and back to the patient through a CRRT extracorporeal dialysis circuit including a dialyzer having semi-permeable dialysis membranes and a dialysate chamber surrounding the membranes;
b) flowing a dialysis fluid containing calcium and citrate through the dialysate chamber of the dialyzer, wherein the dialysis fluid comprises 2-8 mM citrate and 1-5 mM total calcium;
c) introducing a citrate containing anticoagulation fluid into the blood upstream of the dialyzer in a sufficient amount to reduce clotting of the patient's blood in the CRRT extracorporeal dialysis circuit, wherein the citrate containing anticoagulation fluid comprises at least 8 mM citrate; and
d) infusing blood, which has passed through the dialyzer, into the patient.

27. The method of claim 26, wherein the amount of calcium contained in the dialysate is sufficient to reduce the need for calcium to be added to the patient's blood downstream of the dialyzer.

28. The method of claim 26, wherein the citrate comprises sodium citrate.

29. A method of performing regional citrate anticoagulant dialysis of a patient's blood during continuous renal replacement therapy (CRRT), the system comprising:
a) flowing blood from and back to the patient through a CRRT extracorporeal dialysis circuit including a dialyzer having a semi-permeable dialysis membrane and a dialysate side adjacent the membrane;
b) flowing a dialysis fluid comprising 1-5 mM total calcium and 2-8 mM citrate through the dialysate side of the dialyzer; and
c) introducing a citrate containing anticoagulation fluid comprising at least 8 mM citrate into the patient's blood upstream of the dialyzer in a sufficient amount to reduce clotting of the patient's blood in the CRRT extracorporeal dialysis circuit, whereby the patient's blood is dialyzed.

30. The method of claim 29, wherein the amount of calcium contained in the dialysate is sufficient to reduce the need for calcium to be added to the patient's blood downstream of the dialyzer.

31. The method of claim 29, wherein the citrate comprises sodium citrate.

32. The multipart fluid system according to claim 1, wherein said anticoagulation fluid comprises 2.4 mM total calcium.