# Title

Amended Application for an Experimental Use Permit for 1507x59122xMON810,

1507xMON810 and 59122xMON810

Data Requirement

40 CFR Part 172

Authors

Rachel Binning, Keri Henderson, Dave Langer, Scott Mundell, Tracy Rood and Jamie Staley

Submission Date

October 30, 2009

Submitter

Jamie Staley

Pioneer Hi-Bred International, Inc.

2450 SE Oak Tree Court

Ankeny, IA 50021

Submitter Reference Number

PHI-2008-139

Volume 1

# Table of Contents

Page

Statement of Data C onfidentialit y Claim........................................................................................ ...... 2

Transmittal Document .......................................................................................................... .............. 5

EPA Form 8570-17 (Application fo r Experimental Us e Permit ) ......................................................... 7

EPA Form 8570-4 (Confidential Statements of Formula).................................................................... 8

EPA Form 8570-34 (Certification with Respect to Citati on of Data )................................................... 9

EPA Form 8570-35 (Data Matrix for 1507x59122x MON810) ........................................................... 10

EPA Form 8570-35 (Data Matr ix for 1507x MON810) .......................................................................73

EPA Form 8570-35 (Data Matr ix for 59122x MON810) ...................................................................114

Section A. Produ ct Chemistry .................................................................................................. ......155

Section B. Pr oposed Labels .................................................................................................... .......156

Section C. Reference to Availabl e Environmental To xicity Data ....................................................212

Section D. Residue Data ....................................................................................................... .........213

Section E. Effectiveness Data ................................................................................................. .......214

Section F. Tolerance if Testing Involves Use in Food or Feed ......................................................218

Section G. Proposed Experimental Pr ogram .................................................................................219

G1. Participants in t he Experimental Progra m.........................................................................219

G2. Acreage and Seed Quantities by Stat e or Territory for the EUP Pr ogram .......................219

G3. Details of the Proposed EUP Program .............................................................................232

G4. Defi nitions............................................................................................................... ...........232

G5. Universal Prot ocol Procedures..........................................................................................233

Nursery / Breedi ng Protocol.................................................................................................... ..236

Yield / Agrono mic Protocol..................................................................................................... ...237

Efficacy / IR M Prot ocol........................................................................................................ ......238

Seed Producti on Prot ocol ....................................................................................................... ..239

Regulatory Protocol ............................................................................................................ ......240

Confidential Attachment ........................................................................................................ ..........241

# List of Tables and Figures

Tables

Page

Table 1 Cross-refere nce Event Table ........................................................................................... .155

Table 2. Lepidopteran Pests That Are C ontrolled or Suppressed by Pyramided

1507xMON8 10 ..................................................................................................................2 14

Table 3. Efficacy of Breeding Stacks Agai nst First Generation Eu ropean Corn Borer .................216

Table 4. Efficacy of Breeding Stacks Against Fa ll Armywo rm ......................................................217

Table 5. Breeding Stacks Included in Categorie s 1, 2 and 3 ........................................................220

Table 6. Proposed Tota l Acreage by State ....................................................................................22 1

Table 7. Estimated Acreage per State/Te rritory for Nursery / Breeding Pr otocol ..........................222

Table 8. Estimated Acreage per State/Terr itory for Yield / Agr onomic Prot ocol ...........................224

Table 9. Estimated Acreage per State/Te rritory for Efficacy / IRM Prot ocol .................................226

Table 10. Estimated Acreage per State/Territory for Seed Production Protocol............................228

Table 11. Estimated Acreage per State/Territory for Regulatory Protoc ol.....................................229

Table 12. Summary of EUP Pr ogram Quantitie s for 2 009 ............................................................ 231

Figures

Figure 1. Example of Mast er Label Weight Summary ...................................................................235

# Transmittal Document

Submitter

Pioneer Hi-Bred International, Inc.

2450 SE Oak Tree Court

Ankeny, IA 50021

Regulatory Action in Support of Which This Document is Submitted

Amended Application for an Experimental Use Pe rmit with a Plant-Incorporated Protectant

Pursuant to FIFRA §5(a):

Amended Application for an Experimental Use Permit for 1507x59122xMON810,

1507xMON810 and 59122XMON810

29964-EUP-6

Transmittal Date

October 30, 2009

Submitter Reference Number

PHI-2008-139

# EPA Forms 8570-4

Confidential Statement of Formula

{See CBI Cross Reference Number 1}

# EPA Form 8570-35

Data Matrix for 1507x59122xMON810 (see following pages)Form Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page1of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins andthe genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAcute oral toxicity in mice: Cry1FBacillusthuringiensissubsp. aizawai delta endotoxin44691101 68467 PEREffectiveness data forBacillus thuringiensisvar.aizawai Cry1F insect control protein as expressed inmaize 44691102 68467 PERBackground document on resistance management 44691103 68467 PERProduct characterization data forBacillusthuringiensisvar. aizawai: Cry1F control protein asexpressed in maize44714801 68467 PERCharacterization of gene inserts –Bacillusthuringiensisaizawai: Cry1F control protein asexpressed in maize44714802 68467 PEREquivalency of microbial and maize expressedCry1F protein; characterization of test substancesfor biochemical and toxicological studies. In vitrodigestibility of microbialand maize expressed Cry1Fprotein under simulated gastric conditions44714803 68467 PERQuantitative ELISA analysis of Cry1F expressionlevels in maize MPS inbred and hybrid lines 1360,1365, 1366 and 1369 (interim report) 44714804 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page2of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteComparison of the amino acid sequence similarity ofCry1F and PATproteins to KnPER allergen protein44971701 68467 PERMicrobialB.t.Cry1F (truncated) delta-endotoxin:Maize-insect-pest susceptibility study 45020101 68467 PERCharacterization of inserted genes in Cry1F maizeline 150745020102 68467 PERCharacterization of expressed Cry1F protein inmaize tissues (pollen, grain, grain-containing feedand purified maize-expressed Cry1F protein) andmicrobial expressed Cry1F delta-endotoxin bybiological and biochemicalprocedures 45020103 68467 PERQuantitative ELISA analysis of Cry1F and PATexpression levels in and compositional analysis ofmaize inbred and hybrid line 1362 and 150745020104 68467 PEREnvironmental fate of Cry1F protein incorporatedinto soil 45020105 68467 PERCry1FBacillus thuringiensisvar. aizawai deltaendotoxin: an acute toxicity study with theearthworm in an artificial soil substrate45020106 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page3of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteChronic exposure of Folsomia candida to bacteriallyexpressed Cry1F protein45020107 68467 PEROECD 207B.t.Cry1F delta endotoxin: A 48-hour static-renewalacute toxicity test with the Cladoceran (Daphniamagna) using bacterially expressedB.t.Cry1F deltaendotoxin, and pollen from maize expressingB.t.Cry1F delta endotoxin 45020108 68467 PER885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary study with green lacewinglarvae45020109 68467 PER885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary study with the ladybird beetle 45020110 68467 PER885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary toxicity study with parasitichymenoptera45020111 68467 PER71-2Transgenic corn expressingBacillus thuringiensisvar. aizawai (B.t.) Cry1F delta endotoxin: A dietarytoxicity study with Northern bobwhite45020112 68467 PERField surveys of beneficial arthropods associatedwithBacillus thuringiensisCry1F maize45020113 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page4of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins andthe genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEfficacy of Cry1F events TC1360 and TC15074502011468467PERCry1F binding studies4502011568467PERResistance management plan for transgenic maizeexpressing the Cry1F insecticidal protein fromBacillus thuringiensisvar. aizawai45020116 68467 PERSupplement to MRID 44714801: Supplemental data- Product characterization data forBacillusthuringiensisvar. aizawai: Cry1F control protein asexpressed in maize 45020117 68467 PERSupplement to MRID 44691101: Supplemental data- Acute oral toxicity in mice:Bacillus thuringiensisvar. aizawai: Cry1F delta endotoxin45020118 68467 PERPhosphinothricin acetyltransferase (PAT) protein: Invitro digestibility study 45041501 68467 PERNon-target exposure and risk assessment forenvironmental dispersal of Cry1F maize pollen 45041502 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyEPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6-Page5of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins andthe genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of the dietary effect(s) on honeybeedevelopment using bacterially expressedB.t.Cry1Fdelta endotoxin and pollen from maize expressingB.t.Cry1F delta endotoxin 45041503 68467 PERWaiver request: Fish toxicity test with transgenicmaize (corn) containingBacillus thuringiensisvar.aiaqoi (B.t.) Cry1F delta endotoxin45044201 68467 PERHigh dose demonstration of Cry1F events TC1360and TC1507: European corn borer45131101 68467 PERToxicity of the Cry1F protein to neonate larvae of themonarch buttefly (Danaus plexippus (Linneaus)) 45131102 68467 PERPublic interest document for Cry1F-protected corn 4513110329964OWNThermolability of Cry1F (truncated) delta endotoxin 4527480168467PERCompositional analysis of maize MPS hybrid line150745274802 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page6of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteCry1F lateral flow test kit procedure for analyzingCry1F corn grain 45279301 68467 PERMethod validation report for the determination ofCry1F delta endotoxin protein in grain by EnzymeLinked Immunosorbent Assay 45279302 68467 PERPublic interest document for Cry1F protected corn 4529810129964OWNSupplement to MRID 45131102: Supplemental data- High dose demonstration of Cry1F events TC1360and TC1507: European corn borer45307701 68467 PERWaiver request: Fish toxicity test to assess thepotential effects of maize containingBacillusthuringiensisvar. aizawai (Bt) Cry1F insecticidalprotein (ICP) in native fish 45307702 68467 PERSupplement to MRID 45020109: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with green lacewing larvae45307801 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page7of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSupplement to MRID 45020110: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with green ladybird beetle 45307802 68467 PERSupplement to MRID 45020111: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with parasitic hymenoptera45307803 68467 PERSupplement to MRID 45020106: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: An acutetoxicity study with the earthworm in an artificial soilsubstrate 45307804 68467 PERSupplement to MRID 45041503: Evaluation of thedietary effect(s) on honeybee development usingbacterially expressedB.t.Cry1F delta endotoxin andpollen from maize expressingB.t.Cry1F deltaendotoxin45307805 68467 PERSupplement to MRID 45298101: Supplemental data- Public interest document for Cry1F protected corn 45309401 29964 OWNExposure and risk assessment of Herculex I Bt fieldcorn pollen to Karner blue butterfly45512901 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page8of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteNutritional equivalency ofB.t.Cry1F maize - poultryfeeding study 45622001 68467 PERField survey of beneficial arthropods associated withBacillus thuringiensisCry1F maize45648001 68467 PERField surveys of non-target invertebrate populationsinB.t.corn 45652001 68467 PERDevelopment and characterization of EnzymeLinked Immunosorbent Assay (ELISA) for detectionof Cry1F protein45685601 68467 PERIndependent laboratory validation of method GRM02.13, determination of Cry1F delta endotoxinprotein in corn grain by an of Enzyme-LinkedImmunosorbent Assay 45685602 68467 PERSupplement to MRID 45131102: Toxicity of theCry1F protein to neonate larvae of the monarchbutterfly (Danaus plexippus (Linneaus)) 45759701 68467 PERStewardship of Herculex I Insect Protection withrespect to the secondary lepidopteran pest, westernbean cutworm (Richia albicostaSmith)45896601 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page9of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteLack of cross reactivity between Cry1F protein inHerculex I maize and the dust mite Der p7 proteinwith human sera positive for Der p7-IgE46444001 68467 PERMonitoring the susceptibility of European corn borerto Cry1Ab and Cry1F Bt proteins: Results from the2004 collections and diapausing larvae collected in2003 46583101 80778 PERStewardship of event TC1507 maize with respect tothe secondary lepidopteran pests lesser corn stalkborer (Elasmopalpus lignosellusZeller), southerncorn stalk borer (Diatraea crambidiodesGrote), andsugarcane borer (Biatraea saccharalisFabricius) 4660020168467PERSlide presentation summarizing European cornborer and Cry1F resistance monitoring update46695801 68467 PERInsect resistance management complianceassurance program report for corn borer-protectedBt corn 46747801 80778 PERField surveys of non-target invertebrate populationsin Bt corn: Supplement to MRID No. 4565200146784601 80778 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page10of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteMonitoring the susceptibility of corn lepidopteranpests to Cry1Ab and Cry1F proteins: 2005monitoring results46874901 80778 PERResearch results on 2004 European corn borercollections from Hamilton County, Iowa: Cry1F 47011201 68467 PERInsect resistance management complianceassurance program report for corn borer-protectedBt corn, corn rootworm-protected Bt corn, and cornborer/corn rootworm protected stacked Bt corn 47044401 80778 PERMonitoring the susceptibility of corn lepidopteranpests to Cry1Ab and Cry1F proteins: 2006monitoring results47118401 80778 PERSoil accumulation of Cry1F protein after three yearsof cropping with Herculex I corn 47120701 68467 PERTC1507 maize and fall armyworm in Puerto Rico 4717600168467PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page11of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct characterization data forBacillusthuringiensisPS149B1 13.6 kDa and 43.8 kDainsecticidal crystal proteins expressed in transgenicmaize plants 45242201 68467 PEREquivalency of microbial and maize-expressedPS149B1 proteins45242203 68467 PERMicrobial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study45242204 68467 PERComparison of the amino acid sequence of theBacillus thuringiensisstrain PS149B1 13.6 kDa and43.8 kDa insecticidal crystal proteins to KnPERprotein allergens 45242205 68467 PERCharacterization ofPseudomonasproduced andtransgenic maize expressed phosphinothricinacetyltransferase (PAT) protein45242206 68467 PERPS149B1 14 kDa protein: Acute oral toxicity study inCD-1 mice45242207 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page12of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NotePS149B1 44 kDa protein: Acute oral toxicity study inCD-1 mice 45242208 68467 PERPS149B1 14 kDa and 44 kDa proteins: Acute oraltoxicity study in CD-1 mice 45242209 68467 PERPS149B1 binary insecticidal crystal protein: A dietarytoxicity study with ladybird beetle 45242210 68467 PERThe tri-trophic interaction between PS149B1transformed maize, corn leaf aphid and ladybirdbeetle 45242211 68467 PERIn vitro digestibility ofPS149B1 proteins 45242212 68467 PERQuantitative ELISA analysis of PS149B1 proteinexpression levels in hybridand inbred lines of maizeevent TC5639 (interim report) 45242213 68467 PERDegradation of microbial binary PS149B1 deltaendotoxin in a representative soil from the midWestern USA maize growing region45242214 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page13of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct durability plan for transgenic maizeexpressing insecticidal crystal protein fromBacillusthuringiensisstrain PS149B1 during theexperimental use period 45242215 68467 PERField efficacy of PS149B1 maize events against cornrootworms 45242216 68467 PERMicrobial PS149B1 binary insecticidal crystalproteins, pollen expressing PS149B1 binaryinsecticidal crystal proteins, and individual PS149B114 kDa and 44 kDa insecticidal crystal proteins 45340701 68467 PerPS149B1binary insecticidal crystal protein: Acutetoxicity study to the earthworm in an artificialsubstrate45360201 68464 PERLateral flow test kit method validation for thedetection of the PS149B1 14 kDa and 44 kDaprotein in maize grain45383401 68467 PERHeat lability of individual proteins of the PS149B1binary ICP45584501 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page14of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteIn vitro simulated gastric fluid digestibility study ofmicrobially derived Cry34Ab1 protein45584502 68467 PERCharacterization of Cry34Ab1 and Cry35Ab1 fromrecombinantPseudomonas flourescensandtransgenic maize 45790401 68467 PERCharacterization of DNA inserted into transgeniccorn events (Cry34Ab1 and Cry35Ab1) 45790402 68467 PERPS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout,OncorhynchusmykissWallbaum45790403 68467 PERPS149B1 binary insecticidal crystal protein: Acutetoxicity study with Daphnid,Daphniamagna Straus 45790404 68467 PERPS149B1 binary insecticidal crystal protein: Dietarytoxicity to parasitic hymenoptera (Nasoniavitripennis)45790405 68467 PERAssessment of the chronic toxicity of diet containingBacillus thuringiensisPS149B1 insecticidal crystalprotein to collembolan (Folsomia candida) 45790406 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6-Page15of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NotePS149B1 binary insecticidal crystal protein: Dietarytoxicity to green lacewing (Chrysoperla carnea)45790407 68467 PERSDS-PAGE sensitivity analysis for Cry35Ab1 insupport of the simulated gastric fluid digestibilitystudy MRID 4524221245790408 68467 PERTrait durability and experimental use of transgenicmaize expressing the insecticidal crystalline proteinsCry34Ab1and Cry35Ab1 45790509 68467 PERField efficacy of Cry34Ab1/Cry35Ab1 maize eventsagainst corn rootworms45790410 68467 PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17658)45790501 68467 PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662)45790601 68467 PERSummary heat lability studies withCry34Ab1/Cry35Ab145808601 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page16of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP17658 45833101 68467 PERQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP17662 45833102 68467 PERSlide presentation summarizingCry34Ab1/Cry35Ab1 heat inactivation studies 45860201 68467 PERProbe MOA studies to assess potential for proteinsynthesis inhibition byBacillus thuringiensisPS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbitreticulocyte assay: Re-examination of lab notebookdata45942801 68467 PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662) 46030001 68467 PERPublic interest document for Cry34/35Ab1 cornrootworm-protected corn 46122701 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-Page17of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteIndependent laboratory validation Pioneer Hi-BredInternational, Inc. ELISA method for thequantification of Cry34Ab1 protein from transgenicplants 46123901 68467 PERIndependent laboratory validation of DowAgroSciences method GRM 03.13, “ Determinationof Cry35Ab1 insecticidal crystal protein in maizetissue by Enzyme-Linked Immunosorbent Assay” 46123902 68467 PERCry34/35 protein distribution and familiarity4612390368467PERAgronomic characteristics, quantitative ELISA andnutrient composition analysis of hybrid maize linescontaining cry34Ab1, cry35Ab1 and pat genes: Chilelocations 46123904 68467 PERBiological equivalency of Cry34/35Ab1 insecticidalcrystal protein in transgenic plants derived fromtransgenicPseudomonas flourscens46123905 68467 PERCharacterization of Cry34Ab and Cry35Ab1 proteinsderived from transgenic maize event E4497.59.1.22(DAS-59122-7) 46123906 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6-Page18of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteCharacterization of phosphinothricinacetyltransferase (PAT) derived from transgenicmaize event E4497.59.1.22 46123907 68467 PERCharacterization of DNA inserted into transgeniccorn events DAS-45216-6 and DAS-59122-7 46123908 68467 PERDetailed characterization of DNA into transgeniccorn events DAS-45216-6 and DAS-59122-7 46123909 68467 PEREvaluation of microbederived Cry34Ab1 andCry35Ab1 proteins for protein synthesis inhibitionactivity 46123910 68467 PERNutritional equivalency study of maize containingCry34Ab1 and Cry35Ab1: Poultry feeding study46123911 68467 PERThe effect of Cry34Ab1/Cry35Ab1 proteins on thedevelopment and mortality of the ladybird beetleColeomegilla maculataDeGeer 46123912 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page19of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteNon-target invertebrate ecological risk assessmentfor field corn expressing Cry34Ab1 and Cry35Ab1insecticidal crystal proteinsin event DAS-59122-7 46123913 68467 PEREvaluation of the impact corn rootworm controlstrategies on non-target arthropods46123914 68467 PERInvestigations into dose of Cry34Ab1/Cry35Ab1rootworm-resistant maize event DAS-59122-7against western and northern corn rootworms insupport of trait durability plans 46123915 68467 PEREffect on western corn rootworm adults feeding onCry34/35Ab1 corn rootworm-protected corn tissueand implication for product durability 46123916 68467 PEREvaluation of endangered/threatened insect speciesrelative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids 46123917 68467 PERTrait durability plan for Cry34/35 corn rootwormprotected corn event DAS-59122-7 followingcommercialization 46123918 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page20of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSimulations of corn rootworm adaptation to Cry34/35corn rootworm protected corn in support of traitdurability plans for event DAS-59122-7 46123919 68467 PERDigestion of allergenic and non-allergenic proteins insimulated gastric fluid 46123920 68467 PERDigestion efficiency of allergens and non-allergensin simulated gastric fluid:Bacillus thuringiensisCry34/35Ab1 construct PHP17662 46388601 68467 PERLack of Cry34Ab1/Cry35Ab1 co-association insolution46556801 68467 PEREvaluation of the sequencesimilarities of theCry34Ab1, Cry35Ab1 and PAT proteins to the publicprotein sequence datasets46584701 68467 PERSummary report of a carabid beetle laboratorytoxicity study using Cry34Ab1 and Cry35Ab1including copies of references46714101 68467 PERPreliminary resistance monitoring plan forCry34/35Ab1 corn event DAS-59122-746769201 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page21of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteThermolability of PS149B1 binary delta endotoxin 4535840168467PERSummary information in support of the Section 3registration of Herculex XTRA Insect Protection corn46297101 68467 PERCharacterization of a stacked hybrid of transgeniccorn lines DAS-01507-1 and DAS-59122-7 bycomparison to the individual lines46297102 68467 PERAgronomic characteristics, quantitative ELISA andnutrient composition analysis of hybrid maize linescontaining cry1F, cry34Ab1, cry35Ab1 and patgenes: US and Canada locations 46297103 68467 PERAmended report:: Agronomic characteristics,quantitative ELISA and nutrient composition analysisof hybrid maize lines containing cry1F, cry34Ab1,cry35Ab1 and pat genes: US and Canada locations 46343801 68467 PERNutritional equivalency study of stacked hybrid oftransgenic maize line 1507 (event DAS-01507-1)and 59122 (event DAS-59122-7): Poultry feedingstudy 46343803 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6-Page22of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteComparison of insect efficacy and expression inCry34/35 corn events DAS-59122-7 and DAS59132-8 expressed as a single and a stack withDAS-01507-1 46343804 68467 PERField efficacy of Herculex XTRA (DAS-01507-1xDAS-59122-7) insect resistant corn46343805 68467 PERInvestigation of potential interaction between Cry1Fand the binary Cry34Ab1/Cry35Ab1 proteins46343806 68467 PERThe effect of maize pollen expressing the Cry1F andCry34Ab1/Cry35Ab1 proteins on the developmentand mortality of the ladybird beetleColeomegillamaculataDeGeer 46343807 68467 PERLaboratory bioassay assessment of corn pollenprotein (Cry34/35 and Cry1F, stacked transgenes),and their effect on the larvae of monarch butterfly 46343808 68467 PEREquimolar ratio of Cry1F (tr) + Cry35Ab1+Cry34Ab1: Acute oral toxicity study in CD-1 mice 46384301 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page23of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteDetailed resistance monitoring plan for Cry34/35Ab1corn event DAS-59122-7 47334201 68467 PERProposed revisions to IRM-related registrationrequirements for Cry1 Plant IncorporatedProtectants in field corn47407001 80778 PERProduct Chemistry:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants42994501 524 PERToxicology Summary:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants42994502 524 PEREffectiveness Data:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants 42994503524PERAssessment of the in vitro digestive fate ofBacillusthuringiensissubsp.kurstaki(B.t.k.) HD-1 protein43439201 524 PEREvaluation of the dietary effects of purified B.t.k.endotoxin proteins on honeybee larvae 43439202 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page24of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of the dietary effects of purified B.t.k.endotoxin proteins on honeybee larvae 43439202 524 PEREvaluation of the dietary effects of purified B.t.k.endotoxin proteins on honeybee adults 43439203 524 PERAcute oral toxicity study of Btk HD-1 tryptic coreprotein in albino mice43468001 524 PERStability of the Cry1Ab insecticidal protein ofBacillusthuringiensisvar.kurstaki(B.t.k. HD-1) in sucroseand honey solutions under non-refrigeratedtemperature conditions43468002524PERActivated Btk HD-1 protein: A dietary toxicity studywith green lacewing larvae43468003 524 PERActivated Btk HD-1 protein: A dietary toxicity studywith parasitic hymenoptera (Brachymeria intermedia)43468004 524 PERActivated Btk HD-1 protein: A dietary toxicity studywith ladybird beetles 43468005 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page25of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteCompositional comparison ofBacillus thuringiensissubsp.kurstakiHD-1 protein produced in ECBresistant corn and the commercial product, DIPEL®43533203524PERAssessment of the equivalence of theBacillusthuringiensissubsp.kurstakiHD-1 proteinproduced inEscherichia coliand European cornborer resistant corn43533204524PERA dietary toxicity study with MON80187 meal in thenorthern bobwhite 43533205 524 PERMolecular characterization of insect protected cornline MON81043665501 524 PEREvaluation of insect protected corn lines in 1994U.S. field test locations43665502 524 PERAssessment of the equivalence of B.t.k. HD-1protein produced in several insect protected cornlines andEscherichia coli43665503 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page26of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of the European corn borer resistancecorn line MON801 as a feed ingredient for catfish43887901 524 PERCry1Ab insecticidal protein: an acute toxicity studywith the earthworm in an artificial soil substrate43887902 524 PEREffect of theBacillus thuringiensisinsecticidalproteins Cry1Ab, Cry1Ac, CryII and CryIIIA onFolsomia candida, andXenylla grisea(Insecta:collembola) 43941601 524 PERSupplemental submissionon the tissue expressionand corn earworm (Helicoverpa zea) efficacy of theCry1Ab protein in insect protected corn44168601 524 PERChronic exposure ofFolsomia candidato corn tissueexpressing Cry1Ab protein44271501 524 PERPublic interest document for plant pesticidesBacillusthuringiensissubsp.kurstakiinsect control proteinas expressed in corn 44288901 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-Page27of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810 cornIngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSubmission of efficacy data in support of registrationof MON810 Yielgard®446432002524 PERBacillus thuringiensissubsp.kurstakiHD-1insecticidal protein (B.t.k. HD-1 protein) shares nosignificant sequence similarity with proteinsassociated with allergy or celiac disease 45384901 524 PERValidated method for extraction and direct ELISAanalysis of Cry1Ab protein in corn grain 45415401 524 PERPotential risk to endangered lepidoptera fromYieldgard corn pollen (Bt corn events MON810 andBt11) with emphasison the Karner blue butterfly45512201 524 PERComparison of broiler performance when fed dietscontaining Yieldgard®corn, Yieldgard®x Roundup®Ready parental line or commercial corn hybrid 45611801 524 PERCry1Ab protein levels in leaf, pollen, whole plant,forage, root and grain tissues from corn eventMON810 produced in U.S. field trials in 200245878901 524 PERSignatureName and TitleJamie StaleyRegistration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page28of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAssessment of the potential for persistence andaccumulation of Cry1Ab protein in soil as a result ofsustained Bt corn use 46022401 524 PEREffects on monarch butterfly larvae after continuousexposure to Cry1Ab-expressing corn pollen duringanthesis 46162001 524 PERPetition for exemption from the requirement of atolerance forBacillusthuringiensisCry1, Cry2, andCry3 classes of proteins and the genetic materialnecessary for the production of these proteins in oron all raw agricultural commodities when used asplant-pesticide active ingredients PP 7F4888 524 PEREvaluation of the potential effects of pollen frommaize plants containing the events 1507, 59122 andMON810 to larvae of the Monarch butterfly, Danausplexippus. Lepidoptera, Danaidae 47510902 29964 OWNExpressed trait protein concentration of maize linescontaining events DAS-Ø15Ø7-1, DAS-59122-7,MON-ØØ81Ø-6 and combined trait product DASØ15Ø7-1xMON-ØØ81Ø-6, DAS-59122-7x MONØØ81Ø-6 and DAS-Ø15Ø7-1xDAS-59122-7xMONØØ81Ø-6; Greenhouse47510904 29964` OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page29of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteDurability plan during the EUP phase for MON810crossed with events 1507, 59122 and 1507x5912247510901 29964 OWNLiterature references for Experimental Use Permit:1507xMON810, 1507x59122xMON810 and59122xMON810 47510905 29964 OWNMolecular characterization of combined trait product1507x59122xMON810xNK603 using Southern blotanalysis and event-specific polymerase chainreaction 4767780129964OWNLaboratory characterization of key lepidopteranresponse to pyramided events47677802 29964 OWNField efficacy, toxin/allergen homology, and traitdurability supplemental information for1507x59122xMON810, 1507xMON810, and59122xMON810 47677803 29964 OWNLiterature references supporting the application forRegistration of 1507x59122xMON81047677804 29964 OWNSignatureName and TitleJamie StaleyRegistration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page30of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteComparison of the amino acid sequence identitybetween the Cry1F protein and known proteinallergens47786401 29964 OWNComparison of the amino acid sequence identitybetween the Cry34Ab1 and Cry35Ab1 proteins andknown protein allergens47786402 29964 OWNComparison of the amino acid sequence identitybetween the PAT protein and known proteinallergens 47786403 29964 OWNComparison of the amino acid sequence identitybetween the Cry1Ab protein from event MON810and known protein allergens 47786404 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry1F protein to the NCBI protein sequencedatasets 47786405 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry1Ab protein from event MON810 to the NCBIprotein sequence datasets 47786406 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page31of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of the amino acid sequence similarity ofthe PAT protein to the NCBI protein sequencedatasets 47786407 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry34Ab1 and Cry35Ab1 proteins to the NCBIprotein sequence datasets47786408 29964 OWNAdditional addendum to expressed trait proteinconcentration of maize lines containing events DASØ15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 andcombined trait product DAS-Ø15Ø7-1xMONØØ81Ø-6, DAS-59122-7x MON-ØØ81Ø-6 and DASØ15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6;Greenhouse Submitted 10/8/09 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page1of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Public File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6-Page2of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page3of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page4of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page5of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER29964 OWN68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page6of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER29964 OWN68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-Page7of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER29964 OWN68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page8of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page9of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER80778 PER68467PER68467PER80778PER80778PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page10of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note80778 PER68467 PER80778 PER80778 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page11of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page12of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page13of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page14of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page15of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page16of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page17of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page18of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page19of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page20of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page21of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page22of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page23of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER80778 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page24of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page25of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page26of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page27of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page28of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page29of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page30of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009Form Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page31of31Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:1507x59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009

# EPA Form 8570-35

Data Matrix for 1507xMON810 (see following pages)Form Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page1of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAcute oral toxicity in mice: Cry1FBacillusthuringiensissubsp. aizawai delta endotoxin 44691101 68467 PEREffectiveness data forBacillus thuringiensisvar.aizawai Cry1F insect control protein as expressed inmaize44691102 68467 PERBackground document on resistance management 44691103 68467 PERProduct characterization data forBacillusthuringiensisvar. aizawai: Cry1F control protein asexpressed in maize44714801 68467 PERCharacterization of gene inserts –Bacillusthuringiensisaizawai: Cry1F control protein asexpressed in maize 44714802 68467 PEREquivalency of microbial and maize expressedCry1F protein; characterization of test substancesfor biochemical and toxicological studies. In vitrodigestibility of microbialand maize expressed Cry1Fprotein under simulated gastric conditions44714803 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page2of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteQuantitative ELISA analysis of Cry1F expressionlevels in maize MPS inbred and hybrid lines 1360,1365, 1366 and 1369 (interim report) 44714804 68467 PERComparison of the amino acid sequence similarity ofCry1F and PATproteins to KnPER allergen protein44971701 68467 PERMicrobialB.t.Cry1F (truncated) delta-endotoxin:Maize-insect-pest susceptibility study 45020101 68467 PERCharacterization of inserted genes in Cry1F maizeline 150745020102 68467 PERCharacterization of expressed Cry1F protein inmaize tissues (pollen, grain, grain-containing feedand purified maize-expressed Cry1F protein) andmicrobial expressed Cry1F delta-endotoxin bybiological and biochemical procedures45020103 68467 PERQuantitative ELISA analysis of Cry1F and PATexpression levels in and compositional analysis ofmaize inbred and hybrid line 1362 and 1507 45020104 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page3of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEnvironmental fate of Cry1F protein incorporatedinto soil 45020105 68467 PERCry1FBacillus thuringiensisvar. aizawai deltaendotoxin: an acute toxicity study with theearthworm in an artificial soil substrate 45020106 68467 PERChronic exposure of Folsomia candida to bacteriallyexpressed Cry1F protein45020107 68467 PEROECD 207B.t.Cry1F delta endotoxin: A 48-hour static-renewalacute toxicity test with the Cladoceran (Daphniamagna) using bacterially expressedB.t.Cry1F deltaendotoxin, and pollen from maize expressingB.t.Cry1F delta endotoxin 45020108 68467 PER885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary study with green lacewinglarvae45020109 68467 PER885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary study with the ladybird beetle 45020110 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page4of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status Note885.4340Cry1FBacillus thuringiensisvar. aizawai deltaendotoxin: A dietary toxicity study with parasitichymenoptera45020111 68467 PER71-2Transgenic corn expressingBacillus thuringiensisvar. aizawai (B.t.) Cry1F delta endotoxin: A dietarytoxicity study with Northern bobwhite45020112 68467 PERField surveys of beneficial arthropods associatedwithBacillus thuringiensisCry1F maize 45020113 68467 PEREfficacy of Cry1F events TC1360 and TC15074502011468467PERCry1F binding studies4502011568467PERResistance management plan for transgenic maizeexpressing the Cry1F insecticidal protein fromBacillus thuringiensisvar. aizawai45020116 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:August 12 2009EPA Reg. No. / File Symbol:29964-EUP-6Page5of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSupplement to MRID 44714801: Supplemental data- Product characterization data forBacillusthuringiensisvar. aizawai: Cry1F control protein asexpressed in maize 45020117 68467 PERSupplement to MRID 44691101: Supplemental data- Acute oral toxicity in mice:Bacillus thuringiensisvar. aizawai: Cry1F delta endotoxin 45020118 68467 PERPhosphinothricin acetyltransferase (PAT) protein: Invitro digestibility study 45041501 68467 PERNon-target exposure and risk assessment forenvironmental dispersal of Cry1F maize pollen45041502 68467 PEREvaluation of the dietary effect(s) on honeybeedevelopment using bacterially expressedB.t.Cry1Fdelta endotoxin and pollen from maize expressingB.t.Cry1F delta endotoxin 45041503 68467 PERWaiver request: Fish toxicity test with transgenicmaize (corn) containingBacillus thuringiensisvar.aiaqoi (B.t.) Cry1F delta endotoxin45044201 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page6of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteHigh dose demonstration of Cry1F events TC1360and TC1507: European corn borer 45131101 68467 PERToxicity of the Cry1F protein to neonate larvae of themonarch buttefly (Danaus plexippus (Linneaus)) 45131102 68467 PERPublic interest document for Cry1F-protected corn 4513110329964OWNThermolability of Cry1F (truncated) delta endotoxin 4527480168467PERCompositional analysis of maize MPS hybrid line150745274802 68467 PERCry1F lateral flow test kit procedure for analyzingCry1F corn grain45279301 68467 PERMethod validation report for the determination ofCry1F delta endotoxin protein in grain by EnzymeLinked Immunosorbent Assay 45279302 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page7of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NotePublic interest document for Cry1F protected corn 4529810129964OWNSupplement to MRID 45131102: Supplemental data- High dose demonstration of Cry1F events TC1360and TC1507: European corn borer 45307701 68467 PERWaiver request: Fish toxicity test to assess thepotential effects of maize containingBacillusthuringiensisvar. aizawai (Bt) Cry1F insecticidalprotein (ICP) in native fish 45307702 68467 PERSupplement to MRID 45020109: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with green lacewing larvae45307801 68467 PERSupplement to MRID 45020110: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with green ladybird beetle45307802 68467 PERSupplement to MRID 45020111: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: A dietarytoxicity study with parasitic hymenoptera 45307803 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page8of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSupplement to MRID 45020106: Cry1FBacillusthuringiensisvar. aizawai delta endotoxin: An acutetoxicity study with the earthworm in an artificial soilsubstrate 45307804 68467 PERSupplement to MRID 45041503: Evaluation of thedietary effect(s) on honeybee development usingbacterially expressedB.t.Cry1F delta endotoxin andpollen from maize expressingB.t.Cry1F deltaendotoxin45307805 68467 PERSupplement to MRID 45298101: Supplemental data- Public interest document for Cry1F protected corn 45309401 29964 OWNExposure and risk assessment of Herculex I Bt fieldcorn pollen to Karner blue butterfly45512901 68467 PERNutritional equivalency ofB.t.Cry1F maize - poultryfeeding study4562200168467PERField survey of beneficial arthropods associated withBacillus thuringiensisCry1F maize4564800168467PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page9of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteField surveys of non-target invertebrate populationsinB.t.corn 45652001 68467 PERDevelopment and characterization of EnzymeLinked Immunosorbent Assay (ELISA) for detectionof Cry1F protein 45685601 68467 PERIndependent laboratory validation of method GRM02.13, determination of Cry1F delta endotoxinprotein in corn grain by an of Enzyme-LinkedImmunosorbent Assay 45685602 68467 PERSupplement to MRID 45131102: Toxicity of theCry1F protein to neonate larvae of the monarchbutterfly (Danaus plexippus (Linneaus)) 45759701 68467 PERStewardship of Herculex I Insect Protection withrespect to the secondary lepidopteran pest, westernbean cutworm (Richia albicostaSmith)45896601 29964 OWNLack of cross reactivity between Cry1F protein inHerculex I maize and the dust mite Der p7 proteinwith human sera positive for Der p7-IgE 46444001 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page10of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteMonitoring the susceptibility of European corn borerto Cry1Ab and Cry1F Bt proteins: Results from the2004 collections and diapausing larvae collected in2003 46583101 80778 PERStewardship of event TC1507 maize with respect tothe secondary lepidopteran pests lesser corn stalkborer (Elasmopalpus lignosellusZeller), southerncorn stalk borer (Diatraea crambidiodesGrote), andsugarcane borer (Biatraea saccharalisFabricius)46600201 68467 PERSlide presentation summarizing European cornborer and Cry1F resistance monitoring update 46695801 68467 PERInsect resistance management complianceassurance program report for corn borer-protectedBt corn 46747801 80778 PERField surveys of non-target invertebrate populationsin Bt corn: Supplement to MRID No. 4565200146784601 80778 PERMonitoring the susceptibility of corn lepidopteranpests to Cry1Ab and Cry1F proteins: 2005monitoring results 46874901 80778 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page11of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteResearch results on 2004 European corn borercollections from Hamilton County, Iowa: Cry1F47011201 68467 PERInsect resistance management complianceassurance program report for corn borer-protectedBt corn, corn rootworm-protected Bt corn, and cornborer/corn rootworm protected stacked Bt corn 47044401 80778 PERMonitoring the susceptibility of corn lepidopteranpests to Cry1Ab and Cry1F proteins: 2006monitoring results 47118401 80778 PERSoil accumulation of Cry1F after three years ofcropping with Herculex 1 corn 47120701 68467 PERTC1507 maize and fall armyworm in Puerto Rico 4717600168467PERProduct Chemistry:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants 42994501 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page12of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteToxicology Summary:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants42994502 524 PEREffectiveness Data:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants 42994503524PERAssessment of the in vitro digestive fate ofBacillusthuringiensissubsp.kurstaki(B.t.k.) HD-1 protein 43439201524PEREvaluation of the dietary effects of purified B.t.k.endotoxin proteins on honeybee larvae43439202 524 PEREvaluation of the dietary effects of purified B.t.k.endotoxin proteins on honeybee adults43439203 524 PERAcute oral toxicity study of Btk HD-1 tryptic coreprotein in albino mice43468001 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page13of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteStability of the Cry1Ab insecticidal protein ofBacillusthuringiensisvar.kurstaki(B.t.k. HD-1) in sucroseand honey solutions under non-refrigeratedtemperature conditions 43468002 524 PERActivated Btk HD-1 protein: A dietary toxicity studywith green lacewing larvae43468003 524 PERActivated Btk HD-1 protein: A dietary toxicity studywith parasitic hymenoptera (Brachymeria intermedia)43468004 524 PERActivated Btk HD-1 protein: A dietary toxicity studywith ladybird beetles43468005 524 PERCompositional comparison ofBacillus thuringiensissubsp.kurstakiHD-1 protein produced in ECBresistant corn and the commercial product, DIPEL®43533203 524 PERAssessment of the equivalence of theBacillusthuringiensissubsp.kurstakiHD-1 proteinproduced inEscherichia coliand European cornborer resistant corn43533204524PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page14of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteA dietary toxicity study with MON80187 meal in thenorthern bobwhite43533205 524 PERMolecular characterization of insect protected cornline MON81043665501 524 PEREvaluation of insect protected corn lines in 1994U.S. field test locations 43665502 524 PERAssessment of the equivalence of B.t.k. HD-1protein produced in several insect protected cornlines andEscherichia coli43665503 524 PEREvaluation of the European corn borer resistancecorn line MON801 as a feed ingredient for catfish43887901 524 PERCry1Ab insecticidal protein: an acute toxicity studywith the earthworm in an artificial soil substrate43887902 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page15of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEffect of theBacillus thuringiensisinsecticidalproteins Cry1Ab, Cry1Ac, CryII and CryIIIA onFolsomia candida, andXenylla grisea(Insecta:collembola) 43941601 524 PERSupplemental submissionon the tissue expressionand corn earworm (Helicoverpa zea) efficacy of theCry1Ab protein in insect protected corn44168601 524 PERChronic exposure ofFolsomia candidato corn tissueexpressing Cry1Ab protein 44271501 524 PERPublic interest document for plant pesticidesBacillusthuringiensissubsp.kurstakiinsect control proteinas expressed in corn 44288901 524 PERSubmission of efficacy data in support of registrationof MON810 Yielgard®446432002 524 PERBacillus thuringiensissubsp.kurstakiHD-1insecticidal protein (B.t.k. HD-1 protein) shares nosignificant sequence similarity with proteinsassociated with allergy or celiac disease 45384901 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page16of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteValidated method for extraction and direct ELISAanalysis of Cry1Ab protein in corn grain45415401 524 PERPotential risk to endangered lepidoptera fromYieldgard corn pollen (Bt corn events MON810 andBt11) with emphasison the Karner blue butterfly 45512201 524 PERComparison of broiler performance when fed dietscontaining Yieldgard®corn, Yieldgard®x Roundup®Ready parental line or commercial corn hybrid 45611801 524 PERCry1Ab protein levels in leaf, pollen, whole plant,forage, root and grain tissues from corn eventMON810 produced in U.S. field trials in 200245878901 524 PERBioinformatics evaluation of the Cry1Ab proteinproduced in corn event MON810 utilizing an allergendatabase45878902 524 PERAssessment of the potential for persistence andaccumulation of Cry1Ab protein in soil as a result ofsustained Bt corn use46022401 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page17of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEffects on monarch butterfly larvae after continuousexposure to Cry1Ab-expressing corn pollen duringanthesis 46162001 524 PERPetition for exemption from the requirement of atolerance forBacillusthuringiensisCry1, Cry2, andCry3 classes of proteins and the genetic materialnecessary for the production of these proteins in oron all raw agricultural commodities when used asplant-pesticide active ingredients PP 7F4888 524 PEREvaluation of the potential effects of pollen frommaize plants containing the events 1507 andMON810 to larvae of the Monarch butterfly, Danausplexippus. Lepidoptera, Danaidae 47510903 29964 OWNExpressed trait protein concentration of maize linescontaining events DAS-Ø15Ø7-1, DAS-59122-7,MON-ØØ81Ø-6 and combined trait product DASØ15Ø7-1xMON-ØØ81Ø-6, DAS-59122-7x MONØØ81Ø-6 and DAS-Ø15Ø7-1xDAS-59122-7xMONØØ81Ø-6; Greenhouse47510904 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page18of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteDurability plan during the EUP phase for MON810crossed with events 1507, 59122 and 1507x5912247510901 29964 OWNLiterature references for Experimental Use Permit:1507xMON810, 1507x59122xMON810 and59122xMON810 47510905 29964 OWNMolecular characterization of corn combined traitproduct 1507x59122xMON810xNK603 corn usingSouthern blot analysis and Polymerase ChainReaction 47677801 29964 OWNLaboratory characterization of key lepidopteranresponse to pyramided events47677802 29964 OWNField efficacy, toxin/allergen homology, and traitdurability supplemental information for1507x59122xMON810, 1507xMON810, and59122xMON810 4768803 29964 OWNLiterature references supporting the application forregistration of 1507x59122xMON810 4768804 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page19of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1Fand Cry1Ab insecticidal proteinsand the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteComparison of the amino acid sequence identitybetween the Cry1F protein and known proteinallergens 47786401 29964 OWNComparison of the amino acid sequence identitybetween the Cry34Ab1 and Cry35Ab1 proteins andknown protein allergens 47786402 29964 OWNComparison of the amino acid sequence identitybetween the PAT protein and known proteinallergens 47786403 29964 OWNComparison of the amino acid sequence identitybetween the Cry1Ab protein from event MON810and known protein allergens 47786404 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry1F protein to the NCBI protein sequencedatasets 47786405 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry1Ab protein from event MON810 to the NCBIprotein sequence datasets47786406 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page20of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1Fand Cry1Ab insecticidal proteinsand the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of the amino acid sequence similarity ofthe PAT protein to the NCBI protein sequencedatasets 47786407 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry34Ab1 and Cry35Ab1 proteins to the NCBIprotein sequence datasets47786408 29964 OWNAdditional addendum to expressed trait proteinconcentration of maize lines containing events DASØ15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 andcombined trait product DAS-Ø15Ø7-1xMONØØ81Ø-6, DAS-59122-7x MON-ØØ81Ø-6 and DASØ15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6;Greenhouse Submitted 10/8/09 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page1of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Public File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page2of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page3of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page4of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page5of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page6of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 7250 NW 62ndAvenue, Johnston, IA 50131-0552Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER29964 OWN68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page7of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page8of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER29964 OWN68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page9of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467PER68467PER29964OWN68467PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page10of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note80778 PER68467 PER68467 PER80778 PER80778 PER80778 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page11of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER80778 PER80778 PER68467 PER68467 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page12of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-Page13of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 7250 NW 62ndAvenue, Johnston, IA 50131-0552Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page14of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page15of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page16of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page17of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page18of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page19of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page20of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny IA 50021Product:1507xMON810IngredientBacillus thuringiensisCry1F and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File Copy

# EPA Form 8570-35

Data Matrix for 59122xMON810 (see following pages)Form Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page1of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct characterization data forBacillusthuringiensisPS149B1 13.6 kDa and 43.8 kDainsecticidal crystal proteins expressed in transgenicmaize plants 45242201 68467 PEREquivalency of microbial and maize-expressedPS149B1 proteins45242203 68467 PERMicrobial PS149B1 binary delta endotoxin: Maizeinsect-pest susceptibility study 45242204 68467 PERComparison of the amino acid sequence of theBacillus thuringiensisstrain PS149B1 13.6 kDa and43.8 kDa insecticidal crystal proteins to KnPERprotein allergens 45242205 68467 PERCharacterization ofPseudomonasproduced andtransgenic maize expressed phosphinothricinacetyltransferase (PAT) protein 45242206 68467 PERPS149B1 14 kDa protein: Acute oral toxicity study inCD-1 mice45242207 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page2of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NotePS149B1 44 kDa protein: Acute oral toxicity study inCD-1 mice45242208 68467 PERPS149B1 14 kDa and 44 kDa proteins: Acute oraltoxicity study in CD-1 mice45242209 68467 PERPS149B1 binary insecticidal crystal protein: Adietary toxicity study with ladybird beetle 45242210 68467 PERThe tri-trophic interaction between PS149B1transformed maize, corn leaf aphid and ladybirdbeetle 45242211 68467 PERIn vitro digestibility ofPS149B1 proteins 45242212 68467 PERQuantitative ELISA analysis of PS149B1 proteinexpression levels in hybridand inbred lines of maizeevent TC5639 (interim report)45242213 68467 PERDegradation of microbial binary PS149B1 deltaendotoxin in a representative soil from the midWestern USA maize growing region45242214 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page3of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct durability plan for transgenic maizeexpressing insecticidal crystal protein fromBacillusthuringiensisstrain PS149B1 during theexperimental use period 45242215 68467 PERField efficacy of PS149B1 maize events against cornrootworms 45242216 68467 PERMicrobial PS149B1 binary insecticidal crystalproteins, pollen expressing PS149B1 binaryinsecticidal crystal proteins, and individual PS149B114 kDa and 44 kDa insecticidal crystal proteins 45340701 68467 PerPS149B1binary insecticidal crystal protein: Acutetoxicity study to the earthworm in an artificialsubstrate 45360201 68464 PERLateral flow test kit method validation for thedetection of the PS149B1 14 kDa and 44 kDaprotein in maize grain45383401 68467 PERHeat lability of individual proteins of the PS149B1binary ICP 45584501 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page4of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteIn vitro simulated gastric fluid digestibility study ofmicrobially derived Cry34Ab1 protein45584502 68467 PERCharacterization of Cry34Ab1 and Cry35Ab1 fromrecombinantPseudomonas flourescensandtransgenic maize45790401 68467 PERCharacterization of DNA inserted into transgeniccorn events (Cry34Ab1 and Cry35Ab1)45790402 68467 PERPS149B1 binary insecticidal crystal protein: An 8-day dietary study with rainbow trout,OncorhynchusmykissWallbaum45790403 68467 PERPS149B1 binary insecticidal crystal protein: Acutetoxicity study with Daphnid,Daphniamagna Straus45790404 68467 PERPS149B1 binary insecticidal crystal protein: Dietarytoxicity to parasitic hymenoptera (Nasoniavitripennis)45790405 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page5of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAssessment of the chronic toxicity of diet containingBacillus thuringiensisPS149B1 insecticidal crystalprotein to collembolan (Folsomia candida) 45790406 68467 PERPS149B1 binary insecticidal crystal protein: Dietarytoxicity to green lacewing (Chrysoperla carnea)45790407 68467 PERSDS-PAGE sensitivity analysis for Cry35Ab1 insupport of the simulated gastric fluid digestibilitystudy MRID 4524221245790408 68467 PERTrait durability and experimental use of transgenicmaize expressing the insecticidal crystalline proteinsCry34Ab1and Cry35Ab145790509 68467 PERField efficacy of Cry34Ab1/Cry35Ab1 maize eventsagainst corn rootworms 45790410 68467 PERProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17658) 45790501 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page6of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662)45790601 68467 PERSummary heat lability studies withCry34Ab1/Cry35Ab145808601 68467 PERQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP1765845833101 68467 PERQuantitative ELISA analysis of Cry34Ab1 andCry35Ab1 proteins expressed in maize plantstransformed with the vector PHP17662 45833102 68467 PERSlide presentation summarizingCry34Ab1/Cry35Ab1 heat inactivation studies 45860201 68467 PERProbe MOA studies to assess potential for proteinsynthesis inhibition byBacillus thuringiensisPS149B1 Cry34Ab1/Cry35Ab1 proteins in rabbitreticulocyte assay: Re-examination of lab notebookdata45942801 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page7of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteProduct characterization data forBacillusthuringiensisCry34Ab1 and Cry35Ab1 proteinsexpressed in transgenic maize plants (PHP17662)46030001 68467 PERPublic interest document for Cry34/35Ab1 cornrootworm-protected corn46122701 29964 OWNIndependent laboratory validation Pioneer Hi-BredInternational, Inc. ELISA method for thequantification of Cry34Ab1 protein from transgenicplants 46123901 68467 PERIndependent laboratory validation of DowAgroSciences method GRM 03.13, “ Determinationof Cry35Ab1 insecticidal crystal protein in maizetissue by Enzyme-Linked Immunosorbent Assay” 46123902 68467 PERCry34/35 protein distribution and familiarity4612390368467PERAgronomic characteristics, quantitative ELISA andnutrient composition analysis of hybrid maize linescontaining cry34Ab1, cry35Ab1 and pat genes: Chilelocations 46123904 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page8of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteBiological equivalency of Cry34/35Ab1 insecticidalcrystal protein in transgenic plants derived fromtransgenicPseudomonas flourscens46123905 68467 PERCharacterization of Cry34Ab and Cry35Ab1 proteinsderived from transgenic maize event E4497.59.1.22(DAS-59122-7)46123906 68467 PERCharacterization of phosphinothricinacetyltransferase (PAT) derived from transgenicmaize event E4497.59.1.22 46123907 68467 PERCharacterization of DNA inserted into transgeniccorn events DAS-45216-6 and DAS-59122-746123908 68467 PERDetailed characterization of DNA into transgeniccorn events DAS-45216-6 and DAS-59122-746123909 68467 PEREvaluation of microbederived Cry34Ab1 andCry35Ab1 proteins for protein synthesis inhibitionactivity 46123910 68467 PERNutritional equivalency study of maize containingCry34Ab1 and Cry35Ab1: Poultry feeding study 46123911 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page9of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteThe effect of Cry34Ab1/Cry35Ab1 proteins on thedevelopment and mortality of the ladybird beetleColeomegilla maculataDeGeer46123912 68467 PERNon-target invertebrate ecological risk assessmentfor field corn expressing Cry34Ab1 and Cry35Ab1insecticidal crystal proteins in event DAS-59122-746123913 68467 PEREvaluation of the impact corn rootworm controlstrategies on non-target arthropods46123914 68467 PERInvestigations into dose of Cry34Ab1/Cry35Ab1rootworm-resistant maize event DAS-59122-7against western and northern corn rootworms insupport of trait durability plans 46123915 68467 PEREffect on western corn rootworm adults feeding onCry34/35Ab1 corn rootworm-protected corn tissueand implication for product durability46123916 68467 PEREvaluation of endangered/threatened insect speciesrelative to the use of Cry34/35Ab1 corn rootwormresistant maize hybrids46123917 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page10of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteTrait durability plan for Cry34/35 corn rootwormprotected corn event DAS-59122-7 followingcommercialization 46123918 68467 PERSimulations of corn rootworm adaptation to Cry34/35corn rootworm protected corn in support of traitdurability plans for event DAS-59122-746123919 68467 PERDigestion of allergenic and non-allergenic proteins insimulated gastric fluid46123920 68467 PERDigestion efficiency of allergens and non-allergensin simulated gastric fluid:Bacillus thuringiensisCry34/35Ab1 construct PHP17662 46388601 68467 PERLack of Cry34Ab1/Cry35Ab1 co-association insolution 46556801 68467 PEREvaluation of the sequencesimilarities of theCry34Ab1, Cry35Ab1 and PAT proteins to the publicprotein sequence datasets46584701 68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page11of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteSummary report of a carabid beetle laboratorytoxicity study using Cry34Ab1 and Cry35Ab1including copies of references46714101 68467 PERPreliminary resistance monitoring plan forCry34/35Ab1 corn event DAS-59122-7 46769201 68467 PERThermolability of PS149B1 binary delta endotoxin 4535840168467PERProduct Chemistry:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants42994501 524 PERToxicology Summary:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants42994502 524 PEREffectiveness Data:Bacillus thuringiensissubsp.kurstaki(B.t.k.) HD-1 protein asexpressed in cornplants 42994503524PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page12of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAssessment of the in vitro digestive fate ofBacillusthuringiensissubsp.kurstaki(B.t.k.) HD-1 protein43439201 524 PEREvaluation of the dietary effects of purified B.t.k.endotoxin proteins on honeybee larvae 43439202 524 PEREvaluation of the dietary effects of purified B.t.k.endotoxin proteins on honeybee adults43439203 524 PERAcute oral toxicity study of Btk HD-1 tryptic coreprotein in albino mice 43468001 524 PERStability of the Cry1Ab insecticidal protein ofBacillusthuringiensisvar.kurstaki(B.t.k. HD-1) in sucroseand honey solutions under non-refrigeratedtemperature conditions 43468002 524 PERActivated Btk HD-1 protein: A dietary toxicity studywith green lacewing larvae 43468003 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page13of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteActivated Btk HD-1 protein: A dietary toxicity studywith parasitic hymenoptera (Brachymeria intermedia)43468004 524 PERActivated Btk HD-1 protein: A dietary toxicity studywith ladybird beetles43468005 524 PERCompositional comparison ofBacillus thuringiensissubsp.kurstakiHD-1 protein produced in ECBresistant corn and the commercial product, DIPEL®43533203524PERAssessment of the equivalence of theBacillusthuringiensissubsp.kurstakiHD-1 proteinproduced inEscherichia coliand European cornborer resistant corn43533204524PERA dietary toxicity study with MON80187 meal in thenorthern bobwhite 43533205 524 PERMolecular characterization of insect protected cornline MON81043665501 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page14of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of insect protected corn lines in 1994U.S. field test locations 43665502 524 PERAssessment of the equivalence of B.t.k. HD-1protein produced in several insect protected cornlines andEscherichia coli43665503 524 PEREvaluation of the European corn borer resistancecorn line MON801 as a feed ingredient for catfish43887901 524 PERCry1Ab insecticidal protein: an acute toxicity studywith the earthworm in an artificial soil substrate 43887902 524 PEREffect of theBacillus thuringiensisinsecticidalproteins Cry1Ab, Cry1Ac, CryII and CryIIIA onFolsomia candida, andXenylla grisea(Insecta:collembola) 43941601 524 PERSupplemental submissionon the tissue expressionand corn earworm (Helicoverpa zea) efficacy of theCry1Ab protein in insect protected corn44168601 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page15of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810 cornIngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteChronic exposure ofFolsomia candidato corn tissueexpressing Cry1Ab protein44271501 524 PERPublic interest document for plant pesticidesBacillusthuringiensissubsp.kurstakiinsect control proteinas expressed in corn 44288901 524 PERSubmission of efficacy data in support of registrationof MON810 Yielgard®44643202524 PERBacillus thuringiensissubsp.kurstakiHD-1insecticidal protein (B.t.k. HD-1 protein) shares nosignificant sequence similarity with proteinsassociated with allergy or celiac disease 45384901 524 PERValidated method for extraction and direct ELISAanalysis of Cry1Ab protein in corn grain 45415401 524 PERPotential risk to endangered lepidoptera fromYieldgard corn pollen (Bt corn events MON810 andBt11) with emphasison the Karner blue butterfly45512201 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page16of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteComparison of broiler performance when fed dietscontaining Yieldgard®corn, Yieldgard®x Roundup®Ready parental line or commercial corn hybrid45611801 524 PERCry1Ab protein levels in leaf, pollen, whole plant,forage, root and grain tissues from corn eventMON810 produced in U.S. field trials in 200245878901 524 PERBioinformatics evaluation of the Cry1Ab proteinproduced in corn event MON810 utilizing an allergendatabase45878902 524 PERAssessment of the potential for persistence andaccumulation of Cry1Ab protein in soil as a result ofsustained Bt corn use46022401 524 PEREffects on monarch butterfly larvae after continuousexposure to Cry1Ab-expressing corn pollen duringanthesis46162001 524 PERPetition for exemption from the requirement of atolerance forBacillusthuringiensisCry1, Cry2, andCry3 classes of proteins and the genetic materialnecessary for the production of these proteins in oron all raw agricultural commodities when used asplant-pesticide active ingredients PP 7F4888 524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page17of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteEvaluation of the potential effects of pollen frommaize plants containing the events 1507, 59122 andMON810 to larvae of the Monarch butterfly, Danausplexippus. Lepidoptera, Danaidae 47510902 29964 OWNExpressed trait protein concentration of maize linescontaining events DAS-Ø15Ø7-1, DAS-59122-7,MON-ØØ81Ø-6 and combined trait product DASØ15Ø7-1xMON-ØØ81Ø-6, DAS-59122-7x MONØØ81Ø-6 and DAS-Ø15Ø7-1xDAS-59122-7xMONØØ81Ø-6; Greenhouse47510904 29964 OWNDurability plan during the EUP phase for MON810crossed with events 1507, 59122 and 1507x59122 47510901 29964 OWNLiterature references for Experimental Use Permit:1507xMON810, 1507x59122xMON810 and59122xMON81047510905 29964 OWNMolecular characterization of corn combined traitproduct 1507x59122xMON810xNK603 corn usingSouthern blot analysis and Polymerase ChainReaction 47677801 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page18of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteLaboratory characterization of key lepidopteranresponse to pyramided events47677802 29964 OWNField efficacy, toxin/allergen homology, and traitdurability supplemental information for1507x59122xMON810, 1507xMON810, and59122xMON810 4768803 29964 OWNLiterature references supporting the application forRegistration of 1507x59122xMON810 47677804 29964 OWNComparison of the amino acid sequence identitybetween the Cry1F protein and known proteinallergens47786401 29964 OWNComparison of the amino acid sequence identitybetween the Cry34Ab1 and Cry35Ab1 proteins andknown protein allergens 47786402 29964 OWNComparison of the amino acid sequence identitybetween the PAT protein and known proteinallergens47786403 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page19of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteComparison of the amino acid sequence identitybetween the Cry1Ab protein from event MON810and known protein allergens 47786404 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry1F protein to the NCBI protein sequencedatasets47786405 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry1Ab protein from event MON810 to the NCBIprotein sequence datasets 47786406 29964 OWNEvaluation of the amino acid sequence similarity ofthe PAT protein to the NCBI protein sequencedatasets 47786407 29964 OWNEvaluation of the amino acid sequence similarity ofthe Cry34Ab1 and Cry35Ab1 proteins to the NCBIprotein sequence datasets 47786408 29964 OWNAddendum to expressed trait protein concentrationof maize lines containing events DAS-Ø15Ø7-1,DAS-59122-7, MON-ØØ81Ø-6 and combined traitproduct DAS-Ø15Ø7-1xMON-ØØ81Ø-6, DAS59122-7x MON-ØØ81Ø-6 and DAS-Ø15Ø7-1xDAS59122-7xMON-ØØ81Ø-6; Greenhouse Submitted 9/18/09 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page20of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry1F, Cry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in corn.Guideline Reference Number Guideline StudyName MRID Number Submitter Status NoteAdditional addendum to expressed trait proteinconcentration of maize lines containing events DASØ15Ø7-1, DAS-59122-7, MON-ØØ81Ø-6 andcombined trait product DAS-Ø15Ø7-1xMONØØ81Ø-6, DAS-59122-7x MON-ØØ81Ø-6 and DASØ15Ø7-1xDAS-59122-7xMON-ØØ81Ø-6;Greenhouse Submitted 10/8/09 29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Agency Internal Use CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page1of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper version.Public File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page2of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page3of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page4of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page5of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page6of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6-Page7of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER29964 OWN68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page8of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page9of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467PER68467PER68467PER68467PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page10of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467 PER68467 PER68467 PER68467 PER68467 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page11of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note68467 PER68467PER68467PER524PER524PER524PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page12of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page13of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page14of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page15of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page16of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note524 PER524 PER524 PER524 PER524 PER524 PERSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page17of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page18of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page19of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWN29964 OWN29964 OWN29964 OWN29964 OWN29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File CopyForm Approved OMB No. 2070-0060UNITED STATES ENVIRONMENTAL PROTECTION AGENCY1200 Pennsylvania Avenue, N.W.Washington, D.C. 20460Paperwork Reduction Act Notice:The public reporting burden for this collection of information isestimated to average 0.25 hoursper response for registrationactivities and 0.25 hours perresponse for reregistration and special review activities, including time for reading the instructions and completing the necessary forms. Send comments regarding the burden estimate or anyother aspect of this collection of information, including suggestions for reducing the burden to: Director, OPPE Information Management Division (2137), U.S. Environmental Protection Agency,1200 Pennsylvania Avenue, N.W., Washington, DC 20460. Do not send the form to this address.DATA MATRIXDate:October 30, 2009EPA Reg. No. / File Symbol:29964-EUP-6Page20of20Applicant's/Registrant's Name & Address:Pioneer Hi-Bred International, Inc., 2450 SE Oak Tree Court, Ankeny, IA 50021Product:59122xMON810IngredientBacillus thuringiensisCry34/35Ab1 and Cry1Ab insecticidal proteins and the genetic material necessary for their production in cornGuideline Reference Number Guideline StudyName MRID Number Submitter Status Note29964 OWNSignatureName and TitleJamie StaleyU.S. Registration ManagerDateOctober 30, 2009EPA Form 8570-35 (9-97) Electronic and Paper versions available. Submit only Paper versionPublic File Copy

# Sections A Through G of the EUP Application

Section A. Product Chemistry

Pioneer is developing two new products that pyrami d Lepidopteran insecticidal corn traits. These

products are being made by crossing registered even ts using conventional breeding. The 1507xMON180

product expresses the Cry1F and Cry1Ab Bt-derived protein, each highly selective and effective in

controlling certain economically important lepidopteran insect pests of field corn. For markets where

control of corn rootworm is necessary, Pionee r is developing the 1507x59122xMON810 product by

adding (also by conventional breeding) a third regist ered event that expresses the Cry34/35Ab1 protein,

active against corn rootworm larvae. The 59122x MON810 breeding stack is not intended as a

commercial product, but rather will be used as a breeding intermediate and/or inbred for product

development of the 1507x59122x MON810 breeding stack.

Combining Cry1F and Cry1Ab brings together two distinct modes of action against key lepidopteran

pests. Research has shown that Cry1F and Cry1Ab do not share any high-affinity binding sites (Perreia

thesis). Pyramiding the 1507 and MO N810 events will reduce the risk of resistance in insects controlled

by both events.

Enclosed is an application for an Experimental Use Permit (EUP) for field testing of insect-resistant corn.

The application is for new (non-registered) combinations of previously registered events: DAS-Ø15Ø7-1

(also known as 1507), DAS-59122-7 (also known as 59122), and MON-ØØ81Ø-6 (also known as

MON810). These combinations are as follows:

1) DAS-Ø15Ø7-1xDAS-59122-7xMO N-ØØ81Ø-6 (1507x59122xMON810)

2) DAS-Ø15Ø7-1xMON-ØØ81Ø-6 (1507xMON810)

3) DAS-59122-7xMON-ØØ81Ø-6 (59122xMON810)

1507 corn expresses the Cry1F protein that is insectic idally active against certain lepidopteran pests of

corn. MON810 expresses the Cry1Ab protein that is likewise active against certain lepidopteran pests of

corn. In a pyramided breeding combination, these two events express two Cr y proteins for enhanced

pest spectrum and insect resistant management durability. Also included in the EUP is a triple breeding

stack that includes the 59122 even t, which expresses the Cry34/35A b1 protein for control of corn

rootworm pests. The 59122xMON810 breeding stack is not intended as a commercial product, but rather

will be used as a breeding intermediate and/ or inbred for product development of the

1507x59122xMON810 breeding stack.

Table 1 specifies the registered events that comprise the combinations that are the subject of the EUP:

Table 1. Cross-reference Event Table

OECD Event

Name Event Name

Abbreviation Product Name Registration

Number PIP Expressed

DAS-Ø15Ø7-1 1507 Herculex 1 

# I 29964-3 Cry1F

DAS-59122-7 59122 Herculex

Rootworm 29964-4 Cry34/35

MON-ØØ81Ø-6 MON810 YieldGard 2 

# Corn

Borer 524-489 Cry1Ab

1 

# YieldGard® is a registered trademark used under license from MONsanto Company.

2 

# Herculex ® I Insect Protection technology by Dow AgroScienc es and Pioneer Hi-Bred. Herculex is a registered

trademarks of Dow AgroSciences LLC.

# Section B. Proposed Labels

The following pages contain the proposed Master Lab el for the EUP, including labels for the EUP Test

Plants (1-3), as well as labels fo r Registered PIPs (4-13) and Non-registered PIPs (14-44) that will be

planted in EUP experiments.

# Master Label

29964-EUP-6

Subparts:

1) 1507 x 59122 x MON810 Corn Seed

2) 1507 x MON810 Corn Seed

3) 59122 x MON810 Corn Seed

4) 1507 Corn Seed

5) 59122 Corn Seed

6) MON810 Corn Seed

7) 1507 x 59122 Corn Seed

8) Bt11 Corn Seed

9) MIR604 Corn Seed

10) MIR162 Corn Seed

11) Bt11 x MIR604 Corn Seed

12) Bt11 x MIR162 Corn Seed

13) Bt11 x MIR162 x MIR604 Corn Seed

14) 1507 x 59122 x MON810 x MIR162 Corn Seed

15) 1507 x 59122 x MON810 x MIR604 Corn Seed

16) 1507 x 59122 x MIR162 x MIR604 Corn Seed

17) 1507 x MON810 x MIR162 x MIR604 Corn Seed

18) 59122 x MON810 x MIR162 x MIR604 Corn Seed

19) 1507 x 59122 x Bt11 x MIR162 x MIR604 Corn Seed

20) 1507 x 59122 x Bt11 x MIR162 Corn Seed

21) 1507 x 59122 x Bt11 x MIR604 Corn Seed

22) 1507 x Bt11 x MIR162 x MIR604 Corn Seed

23) 59122 x Bt11 x MIR162 x MIR604 Corn Seed

24) 1507 x Bt11 x MIR162 Corn Seed

25) 1507 x Bt11 x MIR604 Corn Seed

26) 59122 x Bt11 x MIR162 Corn Seed

27) 59122 x Bt11 x MIR604 Corn Seed

28) 1507 x 59122 x MIR162 Corn Seed

29) 1507 x 59122 x MIR604 Corn Seed

30) 1507 x MON810 x MIR162 Corn Seed

31) 1507 x MON810 x MIR604 Corn Seed

32) 1507 x MIR162 x MIR604 Corn Seed

33) 59122 x MON810 x MIR162 Corn Seed

34) 59122 x MON810 x MIR604 Corn Seed

35) 59122 x MIR162 x MIR604 Corn Seed

36) MON810 x MIR162 x MIR604 Corn Seed

37) 1507 x Bt11 Corn Seed

38) 1507 x MIR162 Corn Seed

39) 1507 x MIR604 Corn Seed

40) 59122 x MIR162 Corn Seed

41) 59122 x MIR604 Corn Seed

42) MON810 x MIR162 Corn Seed

43) MON810 x MIR604 Corn Seed

44) MIR162 x MIR604 Corn Seed

# 1) 1507 x 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, and Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 0036%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0036%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7.......................................................................<0 .00023%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn MON- ØØ81Ø-6 ...........................................................................<0.0 00033%*

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ..........<0.000016%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 2) 1507 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1F and Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event D AS-Ø15Ø7- 1 .......................................................................<0 .0003%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn MON- ØØ81Ø-6 ...........................................................................<0.0 00036%*

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 3) 59122 x MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0045%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7.......................................................................<0 .00023%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn MON- ØØ81Ø-6 ...........................................................................<0.0 00039%*

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 4) 1507 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1F.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ................<0.002%*

* Maximum percent (wt/wt) of total extractable protein in grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 29964-3

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 5) 59122 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain corn rootworm pests: Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 29964-4

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 501313

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened coleopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 6) MON810 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its producti on in corn MON-ØØ81Ø-6 ............................................................................... <0.0070%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 524-489

EPA Establishment Number: 524-MO-002 Contact Person: Tracy Rood

Monsanto Company Phone: (515) 270-4036

700 Chesterfield Parkway North. Email: tracy.rood@pioneer.com

St. Louis, Missouri 63198

NET CONTENTS: _______________ pounds of corn seed

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 7) 1507 x 59122 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F and Cry34/35Ab1.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 0038%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7.......................................................................<0 .00633%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7.......................................................................<0 .00034%*

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00004%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 29964-5

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 8) Bt11 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredient:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN-BTØ11- 1 .......................................................................<0 .0016%*

Inert Ingredient:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ..........<0.000004%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 67979-1

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood

Syngenta Seeds Inc. Phone: (515) 270-4036

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd.

Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 9) MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against corn rootworm pests: mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredient:

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredient:

Phosphomannose isomerase protei n and the genetic material

necessary for its pr oduction in corn .......................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO.: 67979-5

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood

Syngenta Seeds Inc. Phone: (515) 270-4036

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd.

Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened coleopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 10) MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopt eran pests: VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredient:

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Inert Ingredient:

Phosphomannose isomerase protei n and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO: 67979-14

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood

Syngenta Seeds Inc. Phone: (515) 270-4036

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd.

Research Triangle Park, NC 27709

NET CONTENTS: ___________ pounds of corn seed

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 11) Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 .....................................................................<0.0 0019%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00008%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ..........<0.000004%*

Phosphomannose isomerase protei n and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00004%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO: 67979-8

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood

Syngenta Seeds Inc. Phone: (515) 270-4036

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd.

Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 12) Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN-BTØ11- 1 .......................................................................<0 .0011%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR1 62-4 ......................................................................... <0.0102%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ..........<0.000041%*

Phosphomannose isomerase protei n and the genetic material

necessary for its production in corn ........................................................................................... ..........<0.000041%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO: 67979-12

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood

Syngenta Seeds Inc. Phone: (515) 270-4036

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd.

Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 13) Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 .....................................................................<0.0 0025%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR1 62-4 ......................................................................... <0.0165%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 0077%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00003%*

Phosphomannose isomerase protei n and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00065%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA REG NO: 67979-13

EPA Establishment Number: 66736-NC-001 Contact Person: Tracy Rood

Syngenta Seeds Inc. Phone: (515) 270-4036

P.O. Box 12257 Email: tracy.rood@pioneer.com

3054 East Cornwallis Rd.

Research Triangle Park, NC 27709

NET CONTENTS: _______________ pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 14) 1507 x 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pes ts: Cry1F, Cry34/35Ab1, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS- 59122-7 ................................................................... <0 .000 161%**

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MON- ØØ81Ø-6 ................................................................... <0.007 0%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protei n and the genetic material

necessary for its production in corn ........................................................................................... .......... <0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 15) 1507 x 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pes ts: Cry1F, Cry34/35Ab1, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS- 59122-7 ................................................................... <0 .000 161%**

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MON- ØØ81Ø-6 ................................................................... <0.007 0%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protei n and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 16) 1507 x 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F, Cry34/35Ab1, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS- 59122-7 ................................................................... <0 .000 161%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 17) 1507 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MON- ØØ81Ø-6 ................................................................... <0.007 0%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ................<0.002%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 18) 59122 x MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MO N-ØØ81Ø-6 .....................................................................<0.0 070%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protei n and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 19) 1507 x 59122 x Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests : Cry1F, Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS- 59122-7 ................................................................... <0 .000 161%**

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 ..................................................................... <0.0 016%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ...... <0.0000069%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 20) 1507 x 59122 x Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pes ts: Cry1F, Cry34/35Ab1, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS- 59122-7 ................................................................... <0 .000 161%**

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 ..................................................................... <0.0 016%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ...... <0.0000069%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... .......... <0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 21) 1507 x 59122 x Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pes ts: Cry1F, Cry34/35Ab1, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS- 59122-7 ................................................................... <0 .000 161%**

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN-BTØ11- 1 .......................................................................<0 .0016%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ...... <0.0000069%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 22) 1507 x Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 ..................................................................... <0.0 016%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........ <0.000004%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 23) 59122 x Bt11 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN-BTØ11- 1 .......................................................................<0 .0016%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

# STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 24) 1507 x Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn ro otworm pests: Cry1F, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 ..................................................................... <0.0 016%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........ <0.000004%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... .......... <0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 25) 1507 x Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 ..................................................................... <0.0 016%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........ <0.000004%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 26) 59122 x Bt11 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootwo rm pests: Cry34/35Ab1, Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN-BTØ11- 1 .......................................................................<0 .0016%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Inert Ingredients

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 27) 59122 x Bt11 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN-BTØ11- 1 .......................................................................<0 .0016%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 28) 1507 x 59122 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn root worm pests: Cry1F, Cry34/35Ab1, and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ...... <0.0000069%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... .......... <0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying

# 29) 1507 x 59122 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn root worm pests: Cry1F, Cry34/35Ab1 and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-59122- 7....................................................................... <0 .0117%**

Bacillus thuringiensis Cry35b1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS- 59122-7 ................................................................... <0 .000 161%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ...... <0.0000069%**

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 30) 1507 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pest s: Cry1F, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MON- ØØ81Ø-6 ................................................................... <0.007 0%**

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ................<0.002%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... .......... <0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying

# 31) 1507 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MON- ØØ81Ø-6 ................................................................... <0.007 0%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ................<0.002%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 32) 1507 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ................<0.002%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) of dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 33) 59122 x MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn root worm pests: Cry34/35Ab1, Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MO N-ØØ81Ø-6 .....................................................................<0.0 070%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 34) 59122 x MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1, Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MO N-ØØ81Ø-6 .....................................................................<0.0 070%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 35) 59122 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn ro otworm pests: Cry34/35Ab1, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 36) MON810 x MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1Ab, VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MO N-ØØ81Ø-6 .....................................................................<0.0 070%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 37) 1507 x Bt11 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1F and Cry1Ab.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Cry1Ab protein and the genetic material (pZO1502)

necessary for its production in corn event SYN -BTØ11-1 ..................................................................... <0.0 016%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........ <0.000004%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 38) 1507 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1F and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN -IR162-4 ..................................................................... <0.0 0403%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ...............< 0.002%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... .......... <0.00019%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue

Email: tracy.rood@pioneer.com

Johnston, IA 50131NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 39) 1507 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1F and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1F protein and the genetic material (PHI8999)

necessary for its production in corn event DAS- Ø15Ø7-1 .....................................................................<0.0 1415%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN-I R6Ø4-5 .................................................................... <0.00 313%**

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ................<0.002%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............ <0.0006%**

* Maximum percent (wt/wt) of total extractable protein in grain

** Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 40) 59122 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry34/35Ab1 and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 41) 59122 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against corn rootworm pests: Cry34/35Ab1 and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry34Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event DAS-591 22-7......................................................................... <0.0117%*

Bacillus thuringiensis Cry35Ab1 protein and the genetic material (PHP17662)

necessary for its production in corn event D AS-59122-7 .....................................................................<0.0 00161%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredients:

Phosphinothricin acetyltransferase (PAT) protein and the genetic material

necessary for its production in corn ........................................................................................... ........<0.0000069%*

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened coleopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 42) MON810 x MIR162 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran pests: Cry1Ab and VIP3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MO N-ØØ81Ø-6 .....................................................................<0.0 070%*

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ............<0.00019%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of critical habitats of endangered/threatened lepidopteran

species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 43) MON810 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: Cry1Ab and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Cry1Ab protein and the genetic material (PV-ZMBK07)

necessary for its production in corn event MO N-ØØ81Ø-6 .....................................................................<0.0 070%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# 44) MIR162 x MIR604 Corn Seed

EPA EXPERIMENTAL USE PERMIT NUMBER: 29964-EUP-6

This package contains seed for transgenic insect-resistant co rn that produces the following proteins with activity

against certain lepidopteran and corn rootworm pests: VIP3A and mCry3A.

For Experimental Use Only

Not for sale to any person other than a Participant or Cooperator of the EPA-approved Experimental Use

Program. For use only at an application site of a Partic ipant or Coordinator and in accordance with the terms and

conditions of the Experimental Use Permi t (EUP). This labeling must be in po ssession of the user at the time the

corn seed is planted.

Active Ingredients:

Bacillus thuringiensis Vip3Aa20 protein and the genet ic material (pNOV1300)

necessary for its production in corn event SYN-IR162 -4 .......................................................................<0 .00403%*

Bacillus thuringiensis mCry3A protein and the genetic material (pZM26)

necessary for its production in corn event SYN -IR6Ø4-5 ......................................................................<0. 00313%*

Inert Ingredient:

Phosphomannose isomerase protein and the genetic material

necessary for its production in corn ........................................................................................... ..............<0.0006%*

* Maximum percent (wt/wt) in dry grain

CAUTION

Keep Out of Reach of Children

EPA Establishment Number: 29964-IA-001 Contact Person: Tracy Rood

Pioneer Hi-Bred International, Inc. Phone: (515) 270-4036

7300 NW 62 nd 

# Avenue Email: tracy.rood@pioneer.com

Johnston, IA 50131

NET CONTENTS: ___________pounds of corn seed.

DIRECTIONS FOR USE

Use of this seed in a manner inconsistent with the terms of the EUP is a violation of Federal law. This seed may

only be used according to the field protocols as s pecified in the approved EUP program. Participants and

Cooperators must have a copy of each applicable protocol prior to initiating any research with these seeds.

Research plots must not be located within a 1⁄4 mile of cr itical habitats of endangered/threatened lepidopteran and

coleopteran species as listed by the US Fish and Wildlife Service.

STORAGE AND DISPOSAL

Store in a secure, cool, and dry place. Do not contaminat e water, food or feed by storage or disposal. Any seed

not used in these experiments must be returned to the seed provider or disposed of as specified in the field

protocols. Do not reuse seed containers. Ensure that containers are completely empty of seeds before

destroying.

# Section C. Reference to Available Environmental Toxicity Data

Summary of data for individual events

59122, 1507, and MON 810 ar e each registered even ts; extensive data have been submitted to EPA

and have been previously review ed (U.S. EPA, 2001 & 2005). To su mmarize, nontarget organism

testing was performed on each of the single events (o r their respective Bt proteins) with honeybees

(larvae and adults), Daphnia magna , lady beetles, parasitic hy menoptera, green lacewing,

earthworms, and collembolans. No adverse effects were found at environmentally relevant exposures

for any of the species tested. As one of the most publicly recognized insects, monarch butterfly larvae

were also tested for sensitivity to Cry1F and Cry1 Ab, and no significant risk to monarchs was found at

environmentally relevant exposures. Additionally , environmental fate data indicate that Cry1F,

Cry34/35Ab1 and Cry1Ab dissipate rapidly from soil. Therefore accumulation of the proteins in soil

should be minimal, thereby decreasing the likelih ood of exposure to nontarget soil organisms. The

Agency previously concluded that the levels of Cry 1F, Cry 1Ab, and Cry 34/35Ab1 likely to occur in

the environment will not po se unreasonable risk to nontarget organisms, including mammals, birds,

fish, bees, and other invertebrates, based on the required and voluntary data submitted.

Specific data for proposed stack events

Pioneer measured the concentrations of Cry1F, Cry3 4/35Ab1, Cry1Ab and PAT in plant tissues of the

proposed stacked events. Expres sion levels were similar to t hose of the single events (PHI-2008-

006), thereby indicating no increase in potential exposure concentrations compared to the previously

reviewed individual events.

Pioneer also evaluated the effects of pollen from t he proposed stacked events on monarch butterfly

larvae. No adverse effects were seen (PHI-2008- 104 and PHI-2008-105); therefore, we do not expect

any additional risk from the stacked products com pared to single events for non-target lepidoptera.

In addition, Pioneer confirmed that the efficacy of 1507 pyramided with MON810 is additive in nature

(as opposed to synergistic or antagonistic) agains t European corn borer, southwestern corn borer,

corn earworm, and fall armyworm (PHI-2008-014). Because there was no evidence of synergy during

testing of several key target pests, we do not expect synergy to occur in non-target insects.

Based on the lack of environmental and mammalian effe cts in the individual events, the similarity in

expression levels, the lack of synergy seen in additivi ty testing, and the low likelihood of exposure, we

expect no adverse effects from the proposed stacke d products to mammals, birds, fish, bees, other

invertebrates, and plants. Lack of exposure due to the small scale of testing plots and the limited

duration of the EUP also points to a very low lik elihood of risk from the proposed stacked product,

similar to the findings by EPA for the previously registered individual events. In addition, the EUP

Program will be conducted on land that is currently in agricultural us e. Therefore, we do not expect

that the proposed stacked produc t, which may include all of these proteins, will have a different

environmental profile than the i ndividual products that have bee n previously reviewed by EPA.

References

U.S. EPA. 2001. Environmental Assessment: Bt Plant-Incorporated Protectants. Biopesticides

Registration Action Document. October 15, 2001. Accessed June 24, 2008:

http://www.epa.gov/oppbppd1/biopesticides/pips/bt_brad2/3-ecological.pdf

U.S. EPA. 2005. Environmental Assessment: Bacillus thuringiensis Cry34/35Ab1 Corn Biopesticide

Registration Action Document. October 2005. Accessed June 24, 2008:

http://www.epa.gov/pesticides/biopesticides/ingredients/tech_docs/brad_006490.pdf

# Section D. Residue Data

The Cry1F, Cry1Ab and Cry34/35 proteins and the ge netic material necessary for their production in

corn are plant-incorporated protec tants (PIPs). These PIPs are not applied to the corn crop as

conventional pesticides would be, but rather are synthesized by plants carrying the cry1f

, Cry1Ab and

cry34/35 genes. Residue data per se are not available for a PIP. However, for purposes of assessing

dietary and environmental risk of 1507x59122x MON810, 1507xMON810 and 59122XMON810, the

concentrations of Cry1F, Cry1(A)b, Cry34/35 and ph osphinothricin acetyltransferase (PAT) proteins

were determined in different plant tissues at diffe rent plant growth stages using quantitative ELISA

assays. The results from this determination are summarized in Volume 5 of this submission (PHI2008-006).

# Section E. Effectiveness Data

To date, limited efficacy data have been generated on the EUP breeding stacks listed in Table 1.

However, the individual events are well characteri zed with respect to efficacy, and 1507 and MON810

have been demonstrated to be additive in nature, as opposed to synergistic or antagonistic (PHI-2008-

114). Therefore, the breeding pyramids of 1507xMON810 and 1507x59122xMON810 are expected to

be efficacious against the pests listed in Table 2.

Table 2. Lepidopteran Pests That Are Controlled or Suppressed by Pyramided 1507xMON810*

Scientific name Common name 1507

Activity MON810

Activity Predicted

1507 x MON810

Activity

Agrotis ipsilon Black cutworm Yes No Yes

Helicoverpa zea Corn earworm Yes Yes Yes

Ostrinia nubilalis European corn borer Yes Yes Yes

Spodoptera frugiperda Fall armyworm Yes Yes Yes

Diatraea grandiosella Southwestern corn

borer Yes Yes Yes

Richia albicosta Western bean cutworm Yes No Yes

Elasmopalpus

lignosellus Lesser corn stalk borer Yes No Yes

Diatrae crambidoides Southern corn stalk

borer Yes Yes Yes

Diatrea saccharalis Sugarcane borer Yes No Yes

Papaipema nebris Common stalk borer No Yes Yes

* based on current labels

The breeding stacks of 1507x59122xMON810 and 59122xMON810 are expected to be equal in

efficacy to the 59122 event alone against corn rootworm pests: Diabrotica virgifera virgifera, western

corn rootworm (WCRW), Diabrotica berberi , northern corn rootworm (NCRW), and Diabrotica

virgiferea zeae , Mexican corn rootworm (MCRW).

Under the proposed EUP program, efficacy against lepidopteran and coleopteran pests will be

evaluated in a range of environments, gr owing conditions and genetic backgrounds.

Thus far, limited field efficacy trials have been comp leted characterizing first generation European corn

borer, Ostrinia nubilalis (Hubner) (ECB), foliar feeding and fall armyworm, Spodoptera frugiperda (J.E.

Smith) (FAW), whorl feeding on three maiz e hybrids containing events 1507x59122,

1507x59122xMON810, and a negative control (isolin e). The experimental design included nine

# entries (each a single-row plot with 30” row spacin g) arranged in a randomized complete block with

three replications.

For trials characterizing first generation ECB feedi ng, 100 neonates/plant were manually infested three

times within a 7-day period (300 total neonates /plant) beginning when plants reached growth stage

V5. Approximately three weeks after the last infe station, mean damage ratings were taken on either

10 or 15 individual plants per plot, depending on location (Table 3).

For trials characterizing fall armyworm feeding, 20 neonates/plant were manually infested three times

within a 10-day period (60 total neonates/plant) beginning when plants reached growth stage V4.

Approximately two weeks after the last infestati on, mean damage ratings were taken on either 10 or

15 plants per plot, depending on location (Table 4).

Results to date indicate that early vegetativ e-stage maize hybrids co ntaining either events

1507x59122xMON810 or events 1507x59122 were nearly immune to first generation ECB and FAW

foliar injury. Additionally, there were no significant differences in mean damage ratings between

hybrids containing events 1507x59122xMON810 and hy brids with only 1507x59122 for both pests at

all locations.

These results demonstrate the efficacy of these ev ents is stable regardless of stacking or pyramiding

in the event combinations named in Table 1. The EU P will allow for more extens ive efficacy testing to

further characterize the pest spectrum and level of efficacy of named event combinations under a

wider range of environmental conditions.

# Table 3. Efficacy of Breeding Stacks Against First Generation European Corn Borer

Mean damage ratings characterizing first generation ECB foliar feeding on maize hybrids containing

events 1507x59122, 1507x59122xMON810, or a negative c ontrol (isoline) at 5 field trial locations in

2008.

Location Event Combination n a 

# Mean Damage Rating 

Standard Error b,c



# 1507x59122 135 9.0  0.00 a

1507x59122xMON810 135 9.0  0.00 a York, NE

Negative control 135 5.4  0.07 b

1507x59122 135 9.0  0.00 a

1507x59122xMON810 135 9.0  0.00 a Dallas Center, IA

Negative control 135 5.1  0.07 b

1507x59122 90 9.0  0.00 a

1507x59122xMON810 90 9.0  0.00 a Johnston, IA

Negative control 89 5.6  0.07b

1507x59122 135 9.0  0.00 a

1507x59122xMON810 135 9.0  0.00 a Mankato, MN

Negative control 135 5.8  0.09 b

1507x59122 135 9.0  0.00 a

1507x59122xMON810 132 9.0  0.00 a Princeton, IL

Negative control 135 6.0  0.08 b

a 

# n = number of plants characterize d; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15

plants/plot depending on location.

b 

# Damage ratings on individual plants were determined using the following visual rating scale:

9. No visible leaf injury or a small amount of pin or fine shot-hole type injury on a few leaves.

8. Small amount of shot-hole type lesions on a few leaves.

7. Shot-hole injury common on several leaves.

6. Several leaves with shot-hole and elongated lesions (Lesions <0.5” in length).

5. Several leaves with elongated lesions (Lesions 0.5” to 1.0” in length).

4. Several leaves with elongated lesions (Lesions >1.0” in length).

3. Long lesions (>1.0”) common on about one-half the leaves.

2. Long lesions (>1.0”) common on about two-thirds the leaves.

1. Most of the leaves with long lesions.

c 

# Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD

test, P > 0.05).

# Table 4. Efficacy of Breeding Stacks Against Fall Armyworm

Mean damage ratings characterizing FAW fo liar feeding on maize hybrids containing events

1507x59122, 1507x59122xMON810, or a negative control (iso line) at 2 field trial locations in 2008.

Location Event Combination n a 

# Mean Damage Rating 

Standard Error b,c



# 1507x59122 135 8.9  0.02 a

1507x59122xMON810 135 8.9  0.02 a Dallas Center, IA

Negative control 135 2.4  0.07 b

1507x59122 90 9.0  0.00 a

1507x59122xMON810 89 9.0  0.02 a Johnston, IA

Negative control 89 2.8  0.09 b

a 

# n = number of plants characterize d; 3 maize hybrid backgrounds, 3 replications, and either 10 or 15

plants/plot depending on location.

b 

# Damage ratings on individual plants were determined using the following visual rating scale:

9. No damage to pinhole lesions present on whorl leaves.

8. Pinholes and small circular lesions present on whorl leaves.

7. Small circular lesions and a few small el ongated (rectangular shaped) lesions up to 0.5” in

length present on whorl and furl leaves.

6. Several small elongated lesions 0.5” to 1” in length on a few whorl and furl leaves.

5. Several large elongated lesions greater than 1” in length present on a few whorl and furl leaves

and/or a few small to mid-sized uniform to irregular shaped holes (basement membrane

consumed) in whorl and furl leaves.

4. Several large elongated lesions present on seve ral whorl and furl leaves and/or several large

uniform to irregular shaped holes in whorl and furl leaves.

3. Many elongated lesions of all sizes present on several whorl leaves plus several large uniform

to irregular shaped holes in whorl and furl leaves.

2. Many elongated lesions of all sizes present on most whorl and furl leaves plus many mid to

large-sized uniform to irregular shaped holes in whorl and furl leaves.

1. Whorl and furl leaves almost totally destroyed.

c 

# Within a location, means with the same letter are not significantly different (Fisher’s Protected LSD

test, P > 0.05).

# Section F. Tolerance if Testing Involves Use in Food or Feed

Phosphinothricin acetyltransferase (PAT), the mark er protein in 1507 and 59122 corn, is exempt from

the requirement of a tolerance in all plants: 40CFR §174.522.

MON810 does not contain a marker protein.

Cry1F, the PIP in 1507 corn, is exempt from the r equirement of a tolerance in corn: 40CFR §174.520.

Cry34Ab1 and Cry35Ab1, the PIPs in 59122 corn, are exempt from the requirement of a tolerance in

corn: 40CFR §174.506.

Cry1Ab, the PIP in MON810 corn, is exempt from the requirement of a tolerance in all plants: 40CFR

§174.511.

# Section G. Proposed Experimental Program

For the adequate development of insect-protected transgenic plants, it is necessary to properly

characterize the performance of the crop through larg e-scale field testing. In order to thoroughly

evaluate corn plants, experiments must be grown at a large number of locations, with many

replications of each experiment, in order to obtain sufficient data for hybrid evaluation and

advancement. A total of 34,725 acres in 25 states a nd Puerto Rico is being requested for this EUP.

This acreage is justified for the following reasons:

 Placement of experiments with research center s and cooperators is typically not decided until

early spring of any given year. Spring weathe r conditions can affect planting locations.

Therefore, the proposed EUP acreage needs to take into ac count the possibility of experiments

being planted in a variety of locations and states . Because of this need for flexibility, the

proposed acreage is likely an overestimate of the amount of EUP material that will actually be

planted.

 A large number of potential combinations of registered events may be tested. As Table 5

shows, in addition to the three breeding stacks that comprise the EUP test plants, there are 10

individual registered events and registered bree ding combinations (Category 2) that may be

included in experiments for breeding or comparison purposes. Category 3 contains 31 different

breeding combinations that are non-register ed PIPs which could be planted in EUP

experiments. All together there are 44 different events and combinations that could be tested

under this EUP. Even allocating a small number of estimated acres per protocol and/or state for

each of the 44 entries increases t he total acreage request. In fa ct, ~72% of the acreage request

is to account for potential PIP comparators from Categories 2 and 3. In addition, ~1% of the

acreage request is for border rows and non-PIP entries for breeding or comparison purposes

 There are a large number of target pests fo r the EUP test plants (Section G3.)—three corn

rootworm pests and ten lepidopteran pests. In order to conduct adequate testing for these

pests, some of which occur sporadically, m any locations in many states are needed.

 As the EUP test plants advance toward comme rcialization, an increasi ng number of acres are

needed to conduct inbred and hybrid seed production for future product development and

testing. Large numbers of seed production ac res are needed to evaluate the costs of producing

hybrid seed, the phenotypic characteristics of male and female inbreds in different

environments, the yield of different inbred combinations in different environments, etc.

G1. Participants in the Experimental Program

See CBI Cross Reference Number 2

G2. Acreage and Seed Quantities by State or Territory for the EUP Program

Pioneer has endeavored to forecast its acreage needs and field testing program for 2010. The quantity of

acres being requested in this application is requ ired for product development and evaluation. As

recommended in EPA’s “Tips for Plant-Incorporated Protecta nt (PIP) Experimenta l Use Permit (EUP)

Program Submission”, this acreage calcul ation includes the following categories:

 Category 1: EUP Test Plants;

 Category 2: Registered PIPs for breeding or comparison purposes;

 Category 3: Non-registered PIPs;

 Category 4: Registered PIPs (other than thos e listed in Category #2) for comparison purposes

only ( i.e ., “commercial checks”);

 Non-PIP plants (for example, herbicide tolerant or non-transgenic); and

 Border rows, if used (not required).

# Table 5 lists the breeding stacks included in Cate gories 1, 2 and 3. Each of the individual events

listed is registered by EPA and all PIP prot eins have full tolerance exemptions.

Table 5. Breeding Stacks Included in Categories 1, 2 and 3

Category 1

EUP Test Plants Category 2

Registered PIPs for Breeding

or Comparison Purposes Category 3

Non-registered PIPs

1507 x 59122 x MON810 1507 1507 x 59122 x MON810 x MIR162

1507 x MON810 59122 1507 x 59122 x MON810 x MIR604

59122 x MON810 MON810 1507 x 59 122 x MIR162 x MIR604

1507 x 59122 1507 x MON810 x MIR162 x MIR604

Bt11 59122 x MON810 x MIR162 x MIR604

MIR604 1507 x 59122 x Bt11 x MIR162 x MIR604

MIR162 1507 x 59122 x Bt11 x MIR162

Bt11 x MIR604 1507 x 59122 x Bt11 x MIR604

Bt11 x MIR162 1507 x Bt11 x MIR162 x MIR604

Bt11 x MIR162 x MIR604 59122 x Bt11 x MIR162 x MIR604

1507 x Bt11 x MIR162

1507 x Bt11 x MIR604

59122 x Bt11 x MIR162

59122 x Bt11 x MIR604

1507 x 59122 x MIR162

1507 x 59122 x MIR604

1507 x MON810 x MIR162

1507 x MON810 x MIR604

1507 x MIR162 x MIR604

59122 x MON810 x MIR162

591122 x MON810 x MIR604

59122 x MIR162 x MIR604

MON810 x MIR162 x MIR604

1507 x Bt11

1507 x MIR162

1507 x MIR604

59122 x MIR162

59122 x MIR604

MON810 x MIR162

MON810 x MIR604

MIR162 x MIR604



# Many of the breeding stacks listed in Category 3 w ill likely not be planted to any appreciable extent.

However, in order to enable full flexibility in the breeding and testing program, many potential

combinations are listed.

Table 6 provides the maximum acreage per state, and Table 7-11 provides the maximum acreage per

protocol. The allocation of these acres betwee n traits and protocols (Tables 7-11) represents best

estimates as of this date. Weather events, pest infestation levels, unforeseen regulatory data

demands from other countries, and other factors, may necessitate a reallocation of acres between

protocols and traits. Table 12 summarizes the am ount of seed planted and the total estimated amount

of active ingredient to be used in the EUP Program.

# Table 6. Proposed Total Acreage by State

State PIP PIPs for

Comparison

Only* Non-PIP Border Total

AR 509.5 4 9 18 540.5

CA 494 14 8 28 544

CO 1236 6 6 16 1264

GA 387 5 8 17 417

HI 649 1 106 8 764

IA 4347.5 31 124 73 4575.5

IL 4085.5 16 115 39 4255.5

IN 1312.5 14 11 31 1368.5

KS 1223.5 3 5 13 1244.5

LA 30.5 0 1 2 33.5

MI 2842 4 108 17 2971

MN 1645.5 6 10 22 1683.5

MO 680.5 5 9 19 713.5

MS 363 2 3 6 374

NC 389.5 4 4 15 412.5

NE 3345.5 15 115 38 3513.5

OK 30.5 0 1 2 33.5

OR 2530 0 100 0 2630

PA 773 4 4 12 793

PR 145 0 5 5 155

SD 672.5 4 5 14 695.5

TN 686 5 5 15 711

TX 1127.5 5 105 18 1255.5

WA 2490 0 100 0 2590

WI 1161.5 6 4 15 1186.5

Total 33157 154 971 443 34725

* This acreage is for registered PIPs that are used for comparison purposes

only and not intended for breeding. AcresTable 7. Estimated Acreage per State/Territory for Nursery / Breeding Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 Bt11 MIR604 MIR162 Bt11xMIR604 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xBt11xMIR162xMIR604 1507x59122xBt11xMIR162 1507x59122xBt11xMIR604 1507xBt11xMIR162xMIR604 59122xBt11xMIR162xMIR604 1507xBt11xMIR162 1507xBt11xMIR604 59122xBt11xMIR162 59122xBt11xMIR604 1507x59122xMIR162AR 11 13111111111110111111111111111CO 11 13111111111110111111111111111GA5 5 1020111111111110111111111111111HI10 10 204020 8 6 10 2 3 1 2 1 154111111111111111IA 22 48222111111113111111111111111IL 22 48222111111113111111111111111IN 11 13111111111110111111111111111KS 11 13111111111110111111111111111MI 22 48222111111113111111111111111MN 22 48222111111113111111111111111MO 22 48222111111113111111111111111MS 11 13111111111110111111111111111NE 22 48222111111113111111111111111PA 11 13111111111110111111111111111PR10 10 305030 8 6 10 2 3 1 2 1 164111111111111111SD 11 13111111111110111111111111111TN 11 13111111111110111111111111111Total176266* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions(e.g.weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors,may necessitate a reallocation of acres betweenprotocols and traits.Category 1Category 2Category 3Table 7. Estimated Acreage per State/Territory for Nursery / Breeding Protocol*(cont'd)

State 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xBt11 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR111111111111111131 0 445 510 54CO111111111111111131 0 442 24 48GA111111111111111131 0 615 510 71HI111111111111111131 0 1255 510 135IA111111111111111131 0 525 510 62IL111111111111111131 0 525 510 62IN111111111111111131 0 442 24 48KS111111111111111131 0 442 24 48MI111111111111111131 0 525 510 62MN111111111111111131 0 525 510 62MO111111111111111131 0 525 510 62MS111111111111111131 0 442 24 48NE111111111111111131 0 525 510 62PA111111111111111131 0 442 24 48PR111111111111111131 0 1455 510 155SD111111111111111131 0 442 24 48TN111111111111111131 0 442 24 48Total465 0 907114 1021** Category 4 is non-experimental material that will be used for check plots only (not breeding).* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspectionpurposes. Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory dataCategory 3 (cont'd)Category 5Table 8. Estimated Acreage per State/Territory for Yield / Agronomic Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 Bt11 MIR604 MIR162 Bt11xMIR604 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xBt11xMIR162xMIR604 1507x59122xBt11xMIR162 1507x59122xBt11xMIR604 1507xBt11xMIR162xMIR604 59122xBt11xMIR162xMIR604 1507xBt11xMIR162 1507xBt11xMIR604 59122xBt11xMIR162 59122xBt11xMIR604 1507x59122xMIR162AR23 22 55069 12 12 69 3 3 3 3 3 3180484445555555554CA34 33 875102 24 12 102 3 3 3 3 3 3258484445555555554CO115 115 25255345 12 12 345 3 3 3 3 3 3732484445555555554GA15 15 33345 12 12 45 3 3 3 3 3 3132484445555555554IA204 204 46454612 60 24 612 3 3 3 3 3 31326484445555555554IL117 117 26260351 36 12 351 3 3 3 3 3 3768484445555555554IN119 119 27265357 24 12 357 3 3 3 3 3 3768484445555555554KS115 115 26256345 12 12 345 3 3 3 3 3 3732484445555555554MI32 32 77196 12 12 96 3 3 3 3 3 3234484445555555554MN160 160 36356480 12 12 480 3 3 3 3 3 31002484445555555554MO48 48 11107144 12 12 144 3 3 3 3 3 3330484445555555554MS14 14 43242 12 12 42 3 3 3 3 3 3126484445555555554NE88 88 20196264 24 12 264 3 3 3 3 3 3582484445555555554NC19 19 44257 12 12 57 3 3 3 3 3 3156484445555555554PA64 64 14142192 12 12 192 3 3 3 3 3 3426484445555555554SD48 48 11107144 12 12 144 3 3 3 3 3 3330484445555555554TN48 48 11107144 12 12 144 3 3 3 3 3 3330484445555555554TX4545 10100135 12 12 135 3 3 3 3 3 3312484445555555554WI113 112 25250339 12 12 339 3 3 3 3 3 3720484445555555554Total31589444* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocation of acres between protocols andtraits.Category 3Category 1Category 2Table 8. Estimated Acreage per State/Territory for Yield / Agronomic Protocol* (cont'd)

State 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xBt11 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR8484484444848484161 3 3942 810 404CA8484484444848484161 14 5088 2836 544CO8484484444848484161 5 11533 1114 1167GA8484484444848484161 5 3313 1215 346IA8484484444848484161 30 197117 6380 2051IL8484484444848484161 15 12048 2937 1241IN8484484444848484161 13 12077 2431 1238KS8484484444848484161 3 11522 911 1163MI8484484444848484161 3 4692 911 480MN8484484444848484161 5 15243 1215 1539MO8484484444848484161 5 6033 1215 618MS8484484444848484161 2 3211 45 326NE8484484444848484161 14 9538 2836 989NC8484484444848484161 4 3633 1316 379PA8484484444848484161 4 7332 1012 745SD8484484444848484161 4 6022 1012 614TN8484484444848484161 4 6022 1012 614TX8484484444848484161 4 5773 1316 593WI8484484444848484161 6 11373 1316 1153Total3059 143 15804 400 16204** Category 4 is non-experimental material that will be used for check plots only (not breeding).Category 5Category 3 (cont'd)* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspectionpurposes. Environmental conditions (e.g. weather events, pest infestation levels), unforeseen regulatory dataTable 9. Estimated Acreage per State/Territory for Efficacy / IRM Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 Bt11 MIR604 MIR162 Bt11xMIR604 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xBt11xMIR162xMIR604 1507x59122xBt11xMIR162 1507x59122xBt11xMIR604 1507xBt11xMIR162xMIR604 59122xBt11xMIR162xMIR604 1507xBt11xMIR162 1507xBt11xMIR604 59122xBt11xMIR162 59122xBt11xMIR604 1507x59122xMIR162AR1113111111111110111111111111111CO1113111111111110111111111111111HI1113111111111110111111111111111IA1113111111111110111111111111111IL1113111111111110111111111111111IN1113111111111110111111111111111MI1113111111111110111111111111111MN1113111111111110111111111111111NE1113111111111110111111111111111TN1113111111111110111111111111111TX1113111111111110111111111111111Total33110* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions(e.g.weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocation of acres betweenprotocols and traits.Category 3Category 1Category 2Table 9. Estimated Acreage per State/Territory for Efficacy / IRM Protocol* (cont'd)

State 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xBt11 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR111111111111111131 1 451 34 49CO111111111111111131 1 451 34 49HI111111111111111131 1 451 34 49IA111111111111111131 1 451 34 49IL111111111111111131 1 451 34 49IN111111111111111131 1 451 34 49MI111111111111111131 1 451 34 49MN111111111111111131 1 451 34 49NE111111111111111131 1 451 34 49TN111111111111111131 1 451 34 49TX111111111111111131 1 451 34 49Total341 11 49544 539** Category 4 is non-experimental material that will be used for check plots only (not breeding).* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspectionpurposes. Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory dataCategory 3 (cont'd)Category 5Table 10. Estimated Acreage per State/Territory for Seed Production Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 Bt11 MIR604 MIR162 Bt11xMIR604 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total Category 3 Category 4** Total PIP Non-PIP Border Total Non-PIP TotalHI40 40 100180100 60 60 50 5 5 5 5 5 5300 0 0 480100 0100 580IA40 40 5005801500 60 60 505555551700 0 0 2280100 0100 2380IL10 10 9509701600 60 60 505555551800 0 0 2770100 0100 2870NE40 40 5005801500 60 60 505555551700 0 0 2280100 0100 2380MI40 40 5005801500 60 60 505555551700 0 0 2280100 0100 2380OR40 40 6507301600 60 60 505555551800 0 0 2530100 0100 2630TX40 40 100180100 60 60 50 5 5 5 5 5 5300 0 0 480100 0100 580WA20 20 6506901600 60 60 505555551800 0 0 2490100 0100 2590Total449011100 0 0 15590800 16390** Category 4 is non-experimental material that will be used for check plots only (not breeding).Category 5* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes.Environmental conditions (e.g.weather events, pest infestation levels) unforeseen regulatory data demands received from other countries, and other factors, maynecessitate a reallocation of acres between protocols and traits.Category 2Category 1Table 11. Estimated Acreage per State/Territory for Regulatory Protocol*

State 1507x59122xMON810 1507xMON810 59122xMON810 Category 1 Total 1507 59122 MON810 1507x59122 Bt11 MIR604 MIR162 Bt11xMIR604 Bt11xMIR162 Bt11xMIR162xMIR604 Category 2 Total 1507x59122xMON810xMIR162 1507x59122xMON810xMIR604 1507x59122xMIR162xMIR604 1507xMON810xMIR162xMIR604 59122xMON810xMIR162xMIR604 1507x59122xBt11xMIR162xMIR604 1507x59122xBt11xMIR162 1507x59122xBt11xMIR604 1507xBt11xMIR162xMIR604 59122xBt11xMIR162xMIR604 1507xBt11xMIR162 1507xBt11xMIR604 59122xBt11xMIR162 59122xBt11xMIR604 1507x59122xMIR162AR22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5IA22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5IL22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5IN22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5KS22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5LA22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5MN22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5MO22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5NC22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5NE22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5OK22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5SD22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5TX22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5WI22151111111111100.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5Total70140* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspection purposes. Environmental conditions(e.g.weather events, pest infestation levels), unforeseen regulatory data demands received from other countries, and other factors, may necessitate a reallocation of acres betweenprotocols and traits.Category 1Category 2Category 3Table 11. Estimated Acreage per State/Territory for Regulatory Protocol* (cont'd)

State 1507x59122xMIR604 1507xMON810xMIR162 1507xMON810xMIR604 1507xMIR162xMIR604 59122xMON810xMIR162 59122xMON810xMIR604 59122xMIR162xMIR604 MON810xMIR162xMIR604 1507xBt11 1507xMIR162 1507xMIR604 59122xMIR162 59122xMIR604 MON810xMIR162 MON810xMIR604 MIR162xMIR604 Category 3 Total Category 4** Total PIP Non-PIP Border Total NON-PIP TotalAR0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5IA0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5IL0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5IN0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5KS0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5LA0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5MN0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5MO0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5NC0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5NE0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5OK0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5SD0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5TX0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5WI0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.515.5 0 30.51 23 33.5Total217.0 0 427.042 469.0** Category 4 is non-experimental material that will be used for check plots only (not breeding).* The allocation of these acres among various event combinations represents best estimates as of this date and should not be used for inspectionpurposes. Environmental conditions (e.g.weather events, pest infestation levels), unforeseen regulatory dataCategory 5Category 3 (cont'd)

# Table 12. Summary of EUP Program Quantities for 2010

State Category 1 Category 2 Category 3 Cry1F

(gm) Cry34/35Ab1

(gm) Cry1Ab

(MON810)

(gm) VIP3

(gm) mCry3a

(gm) Cry1Ab

(Bt11)

(gm)

AR 513.47 1767.68 2007.58 10.45 134.49 1.15 117.03 3.78 4.04

CA 631.31 2171.72 1355.22 10.17 130.80 1.12 109.34 3.53 2.73

CO 2196.97 6329.97 1877.10 25.63 329.12 2.79 254.42 8.21 3.81

GA 446.13 1195.29 1616.16 7.95 102.32 0.88 87.15 2.81 3.25

HI 1877.10 3063.97 521.89 13.67 174.98 1.46 111.16 3.59 1.07

IA 8838.38 25749.16 2007.58 90.48 1160.96 9.79 860.46 27.76 4.20

IL 10488.22 21893.94 2007.58 85.68 1097.55 9.18 740.95 23.90 4.19

IN 2323.23 6717.17 2007.58 27.21 349.46 2.96 270.47 8.72 4.07

KS 2222.22 6329.97 1746.63 25.38 325.93 2.76 250.37 8.08 3.54

LA 42.09 84.18 130.47 0.63 8.09 0.07 6.65 0.21 0.26

MI 5572.39 16473.06 1877.10 59.09 758.32 6.40 568.86 18.35 3.87

MN 3131.31 8712.12 2007.58 34.18 438.78 3.71 332.31 10.72 4.08

MO 1010.10 2971.38 1746.63 14.05 180.60 1.54 146.26 4.72 3.52

MS 294.61 1144.78 1616.16 7.42 95.61 0.82 85.59 2.76 3.25

NC 395.62 1397.31 1485.69 7.99 102.82 0.88 89.37 2.88 2.99

NE 6666.67 19486.53 2007.58 69.59 892.98 7.54 666.32 21.49 4.15

OK 42.09 84.18 130.47 0.63 8.09 0.07 6.65 0.21 0.26

OR 6144.78 15151.52 0.00 52.95 678.62 5.69 469.70 15.15 0.11

PA 1220.54 3670.03 1616.16 15.98 205.37 1.74 163.87 5.29 3.26

PR 420.88 538.72 260.94 3.06 39.10 0.33 24.79 0.80 0.53

SD 968.01 2946.13 1746.63 13.88 178.39 1.52 145.48 4.69 3.52

TN 951.18 2946.13 1877.10 14.14 181.86 1.55 149.52 4.82 3.78

TX 2424.24 5319.87 1746.63 23.51 301.49 2.54 219.06 7.07 3.54

WA 5808.08 15151.52 0.00 52.05 667.17 5.60 469.70 15.15 0.10

WI 2146.46 6144.78 1485.69 24.11 309.52 2.62 236.54 7.63 3.02

Total 66776.09 177441.08 34882.15 689.87 8852.41 74.69 6582.02 212.32 71.14

Assumptions:

3) The g/kg of seed used for 1507 (0.0024 g/kg Cry1F), 59122 (0.031g/kg Cry34Ab1) and MON810 (0.00027 g/kg Cry1Ab) were based on the values observed

in PHI-2008-006 (MRID 475109-04). The amount of a.i for MIR162 (0.031 g/kg VIP3A), MIR604 (0.001 g/kg mCry3A) and Bt11 (0.002 g/kg Cry1Ab) were based

on the levels presented in Syngenta EUP application for MIR162. Kgs of seed per Category

1) Assumed 100% of the EUP test plants (Category 1) were 1507x59122xMON810 (0.0027g/kg Cry1F, 0.034 g/kg Cry34/35Ab1 and 0.00026g/kg

Cry1Ab). Values based on expression levels observed in PHI-2008-006 (MRID 475109-04).

2) Assumed 100% of the acres in Category 2 and Category 3 were planted to each registered single event (i.e. 1507, 59122, MON810, MIR162 and MIR604)

except for Bt11 (Category 2 only). Total A.I. Quantities

# G3. Details of the Proposed EUP Program

Crop: Corn (Zea mays L.)

Target Pests:

Agrotis ipsilon (Black cutworm)

Helicoverpa zea (Corn earworm or cotton bollworm)

Ostrinia nubilalis (European corn borer)

Spodoptera frugiperda (Fall armyworm)

Diatraea grandiosella (Southwestern corn borer)

Richia albicosta (Western bean cutworm)

Elasmopalpus lignosellus (Lesser corn stalk borer)

Diatrae crambidoides (Southern corn stalk borer)

Diatrea saccharalis (Sugarcane borer)

Papaipema nebris (Common stalk borer)

Diabrotica virgifera virgifera (western corn rootworm)

Diabrotica barberi (northern corn rootworm)

Diagrotica virgifera zeae (Mexican corn rootworm)

Major Geographic Areas: 25 states and Puerto Rico, representing diverse agronomic zones

Sites: Pioneer research centers, seed production fi elds, cooperator fields, privately owned farms and

university farms and testing sites. All EUP Bloc ks will be planted on existing agricultural land.

Period of Use: February 1, 2010 through June 30, 2011.

Protocols for Field Experimentation:

Research planned under the EUP Program will be conducted under the following five protocols:

1. Nursery / Breeding;

2. Yield / Agronomic;

3. Efficacy / IRM;

4. Inbred and Hybrid Seed Production; and

5. Regulatory.

G4. Definitions

EUP Test Plants

Plants that contain 1507x59122xMON8 10, 1507xMON810 or 59122xMON810.

EUP Experiment

An Experiment is the unit of measure used to c oordinate and track specific field activities.

Experiment ID’s are assigned to each Experimen t. Experiments containing EUP Test Plants are

defined as EUP Experiments. EUP Experiments may also contain registered PIPs for breeding or

comparison (if listed on the Master Label), non-r egistered PIPs (if listed on the Master Label),

registered PIPs for comparison only ( i.e ., “commercial checks”), and/or non-PIPs (such as

herbicide-tolerant or non-transgenic corn).

# EUP Block

An area of land in which EUP Experiments are planted (including filler corn, if planted between EUP

Experiments) and may consist of one, several or many EUP Experiments. An EUP block may be

planted directly adjacent to non-EUP experiments or bl ocks. An EUP block is defined by the adjacent

nature of the EUP experiments it contains. .

G5. Universal Protocol Procedures

The following procedures will be used for all EUP Experiments.

Shipping and Labeling

 Each shipment containing EUP seed must contai n a copy of the EUP Master Label. A Master

Label Weight Summary may be used to summarize the amount of seed in the shipment, so long

as it accompanies the EUP Master Label. See Figure 3 for an example of a Master Label Weight

Summary.

 Packaging from seed shipments is typically destroyed after shipments have been received but

may be re-used after cleaning.

Schedule

For the continental U.S., planting of trials generally takes place from mid February through the month

of June. Observations and harvesting generally oc cur after planting through August to November. For

Hawaii and Puerto Rico, there may be multiple plantings and harvest cycles throughout the year.

Planting

 Seed may be planted by hand or machine.

o Dispose of any remaining seed according to accepted disposition methods.

o Do not re-use seed packets.

 No special cleaning of planting equipment is required before or after planting any seed planted

under this EUP.

 Border rows may be used for agronomic purposes, but are not required under this EUP Program.

Marking EUP Experiments

The EUP block will be identified with stakes or other marking options. If equipment available, GPS

coordinates may be used to identify the EUP block.

Gene Containment

No containment or isolation procedures are requi red because the events planted under this EUP are

all individually registered by EPA. In addition, all proteins have fu ll tolerance exemptions.

Harvest Procedures



#  Vegetative material or grain will be harvested by hand or machine.



#  Any retained seed will be clearly labeled and stored in a secured facility.



#  No special cleaning of harvest equipment is required before or after harvesting material planted

under this EUP.

# Final Disposition of Harvested Grain or Vegetative Material

Grain and vegetative material harvested under this EUP may enter food/feed streams through normal

commercial channels.

Post-Trial Monitoring

None required.

Storing EUP Material

 EUP Test Plant seed will be stored in a secure facility with a sign stating that regulated,

genetically modified material is stored inside.

 EUP Test Plant seed will be labeled to enable ready identification of materials. Examples of

labeling include (but are not limited to) color coding, marking, tagging, or otherwise distinguishing

seed to allow immediate recognition.

Termination of the EUP

The EUP Test Plants are expected to receive commercia l registration in the early spring of 2010. This

EUP extension is being submitted as a contingency for the unlikely scenario that the registrations are

delayed and plantings need to occur in the early spring.

In the event this EUP contingency plan is needed and plantings under the EUP occur, there may be

EUP Experiments that do not contain any Category 2 or Category 3 events/combinations. For those

experiments, record keeping will cont inue until such time as the EUP Te st Plants are fully registered at

the federal and state level. At that time, the EUP provisions will terminate.

For experiments planted under this EUP that contai n Category 2 or Category 3 events combinations,

record keeping will continue through the termination of the experiment.

# Figure 1. Example of Master Label Weight Summary



# Master Label Weight Summary

29964-EUP-6

Event Weight (lbs)

1507xMON810 0.1

1507x59122xMON810 0.1

1507 0.05

MON810 0.05

Bt11xMIR162 0.03

Bt11 0.03

MIR162 0.02

59122 0.02

MIR604 0.01

# Nursery / Breeding Protocol

General Objectives

 Introgress events 1507, 59122 and MON810 into elite inbred corn lines.

 Produce research seed for evaluation in fi eld, greenhouse and/or laboratory trials.

 Collect phenotypic data on converted inbred lines.

 Collect plant tissue on converted inbred lines for laboratory analysis.

Description

Inbreds may be hand pollinated (selfed, backcrossed or crossed to tester lines) to produce new inbred

and hybrids for evaluation. Some inbreds may be op en pollinated in isolation to produce seed for

phenotypic analysis. Plants may be treated with herb icides and/or insecticides. Seed is generally

retained for future breedi ng or testing purposes.

Gene Containment

No containment or isolation procedures are required.

Harvest Procedures

 Hand or machine harvest.

 Store retained seed in a clearly labeled and secure facility.

 No special cleaning of harvest equipment is required before or after harvesting material planted

under this EUP. .

Final Disposition of EUP Material

Grain and vegetative material harvested under this EUP may enter food/feed streams through

normal commercial channels.

# Yield / Agronomic Protocol

General Objectives

 Assess the agronomic impact of combini ng the 1507, 59122 and MON810 events in various

hybrid backgrounds under various environm ental and insect pressure conditions.

 Assess the yield and agronomic properties of EUP test inbreds and hybrids compared to other

single and stacked event inbreds and hybrids (both registered and non- registered PIP single

events and breeding stacks), commercial che ck hybrids (registered PIPs), conventional

hybrids, and/or insecticide treatments.

 Assess the herbicide tolerance of EUP Test Plants (including EUP Test Plants that have been

crossed with non-PIP herbicide tolerant lines of corn).

Description

EUP Blocks will generally be planted in multiple locations using ra ndomized block desi gn, strips or

single large blocks depending on study type. Seed ma y be treated with a variety of insecticidal seed

treatments or blended with non-transgenic or tran sgenic corn to evaluate agronomic performance

including yield. Plants may be treated with herbicide and/or insecticides. Commercial checks may be

planted as comparators. Harvested grain is generally not retained for breeding purposes.

Gene Containment

No containment or isolation procedures are required.

Harvest Procedures

 Hand or machine harvest.

 Store retained seed in a clearly labeled and secure facility.

 No special cleaning of harvest equipment is required before or after harvesting material planted

under this EUP.

Final Disposition of EUP Material

Grain and vegetative material harvested under this EUP may enter food/feed streams through

normal commercial channels.

# Efficacy / IRM Protocol

General Objectives

 Assess the efficacy of the EUP Test Plants against key target and secondary pests in corn.

 Generate data for use in the refinement of IR M strategies for corn containing events 1507,

MON810 and/or 59122.

 Characterize the refuge deployment methods for the EUP Test Plants.

Description

Test plots will generally be planted in multiple locati ons using randomized block design, strips or single

large blocks depending on study type. Seed may be treated with a variety of insecticidal seed

treatments or blended with non-transgenic or transg enic corn to evaluate various methods of refuge

deployment. Artificial or natural infestations of lepidopteran and coleopteran may be used in these

trials. Plants may be treated with herbicide and/or insecticides. Harvested grain is generally not

retained for breeding purposes.

Gene Containment

No containment or isolation procedures are required.

Harvest Procedures

 Hand or machine harvest.

 Clean harvest equipment ( i.e ., combine) while in the regulated transgenic site.

 Store retained seed in a clearly labeled and secure facility.

 No special cleaning of harvest equipment is required before or after harvesting material planted

under this EUP.

Final Disposition of EUP Material

Grain and vegetative material harvested under this EUP may enter food/feed streams through

normal commercial channels.

# Inbred and Hybrid Seed Production Protocol

General Objectives

 Increase inbred and hybrid seed for furthertesting and product development in various genetic

backgrounds

Description

EUP Blocks (approximately 0.1 to 15 acres in size ) of individual inbred lines may be increased by

open pollination. Hybrid seed may be produced (in EUP Blocks approximately 1 to 30 acres in size)

by planting two inbred lines and allowing open pollination (by using a male sterile inbred or removing

the tassels from one of the inbreds). Plants may be treated with herbicides and/or insecticides.

Gene Containment

No containment or isolation procedures are required.

Harvest Procedures

 Hand or machine harvest.



 Store retained seed in a clearly labeled and secure facility.

 No special cleaning of harvest equipment is required before or after harvesting material planted

under this EUP.

Final Disposition of EUP Material

 Grain and vegetative material harvested under this EUP may enter food/feed streams through

normal commercial channels..

# Regulatory Protocol

General Objectives:

 Conduct field trials as needed to support global regulatory submissions

 Generate plant tissue samples from EUP Test Plants (inbreds and hybrids) for use in

regulatory characterization studies.

 Collect phenotypic data for use in global regulatory submissions

 Produce grain for use in animal feeding studies

Description

Test plots will generally be planted in multiple locati ons using randomized block design, strips or single

large blocks depending on study type. Agronomic characteristics and plant tissue samples may be

collected at various developmental growth stages. Plants may be treated with herbicide and/or

insecticides.

Gene Containment

No containment or isolation procedures are required:

Harvest Procedures

 Hand or machine harvest.

 Store retained seed in a clearly labeled and secure facility.

 No special cleaning of harvest equipment is required before or after harvesting material planted

under this EUP

Final Disposition of EUP Material

Grain and vegetative material harvested under this EUP may enter food/feed streams through

normal commercial channels.

# CONFIDENTIAL ATTACHMENT

{CBI Cross Reference Number 1}

EPA Form 8570-4

Confidential Statement of Formula

The Certified Limits presented in the attached CSFs represent the highest and lowest levels of

individual proteins measured during the course of studies on events or event combinations for each

plant tissue type. When data meas urements are not available for ev ent combinations, expression of

proteins is assumed to be additive (based on individual events or combinations with known protein

levels). No entries are being made for the No minal Concentration (column 13) of each protein

because their levels vary significantly by tissue ty pe and by growth stages of the plants. There is not

an expected amount of each ingredient in a typical plant sample.

Pioneer is proposing a set of non-standard Certified Limits as allowed under 40 CFR § 158.175(c).

These proteins are plant-incorporat ed protectants or marker proteins produced by the plant, not in a

traditional closely monitored and controlled manufacturi ng facility. Multiple factors beyond the control

of the Applicant could influence the levels of these proteins synthesized by i ndividual plants, including

but not limited to environmental conditions, plant growth stage, and the presence of biotic or abiotic

stressors. For these reasons, t he Applicant is not providing the Certification required by 40 CFR §

158.175(e).

# {CBI Cross Reference Number 2}