USDA, AMS, S&T, Monitoring Programs Division, Microbiological Data Program

3 October 2011 Index of Standard Operating Procedures (SOPs)

Index of SOPs Page 1 of 4

ACTIVE SOPs

ADMINISTRATIVE PROCEDURES (Sampling and Laboratory)

MPO-ADMIN Administrative Procedures for the Microbiological Data Program and

Pesticide Data Program (Rev. 2, 10/03/2011 Effective 01/01/2012)

 Attachment 1. MPO Designated Federal Records Centers

 Attachment 2. Standard Form SF-135 Template

 Attachment 3. Example SF-135 for Routine Data Packages

 Attachment 4. Example SF-135 for Method Validation Data

Packages

 Attachment 5. Example SF-135 for Supporting Documentation

 Attachment 6. Example SF-135 for Sampling Documentation

 Attachment 7. Instructions for Assembly and Packaging of Record

Boxes

 Attachment 8. Form GSA-49, Requisition/Procurement Request for

Equipment Supplies or Services

 Attachment 9. Equipment Inventory

 Attachment 10. Form AD-112, Report of Unserviceable, Lost,

Stolen, Damaged or Destroyed Property

 Attachment 11. Form AD-107, Report of Transfer or Other

Disposition or Construction of Property

SAMPLING

MDP-SAMP-PROC-1 Sampling Plans and Documentation (Rev. 03, 4/29/09)

MDP-SAMP-PROC-2 Sampling Procedures on Site (Rev. 04, 8/31/09)

 Attachment 1. Standard Form SF-135 Template

 Attachment 2. Example SF-135 for Routine Data Packages

 Attachment 3. MPO Designated Federal Records Centers

MDP-SAMP-PROC-3 Packing and Shipment of Samples (Rev. 03, 4/29/09)

MDP-SAMP-PROC-4 Sample Information Forms and Chain of Custody (Rev. 03, 4/29/09)

MDP-SAMP-PROC-5 Sampling Procedures during National or Regional Emergency Events

(Original, 4/29/09)

MDP-SAMP-GLOSSARY Glossary of Sampling Terms (Original, 4/29/09)

LABORATORY OPERATIONS

MDP-LABOP-02 Sample Receipt, Elution, Pre-enrichment and DNA Extraction (Rev. 18,

09/01/11)

 MDP-LABOP-02 Appendix A – Cantaloupe (Original, 07/01/10)

 MDP-LABOP-02 Appendix B – Cilantro (Original, 07/01/10)

 MDP-LABOP-02 Appendix C – Hot Pepper (Original, 07/01/10)

 MDP-LABOP-02 Appendix D – Lettuce (V1, 09/01/11)

 MDP-LABOP-02 Appendix E – Spinach (V1, 09/01/11)

 MDP-LABOP-02 Appendix F – Sprouts (Original, 07/01/10)

 MDP-LABOP-02 Appendix G – Tomatoes (V2, 09/01/11)

 Attachment 1, In-Laboratory Sampling Guidance (Original, 09/01/11)

MDP-LABOP-03 Microbiological Media and Reagents (Rev. 4, 09/01/11)

MDP-LABOP-08 Procedure for Testing and Maintaining Control Strains (Rev. 4, 4/01/09)
USDA, AMS, S&T, Monitoring Programs Division, Microbiological Data Program

3 October 2011 Index of Standard Operating Procedures (SOPs)

Index of SOPs Page 2 of 4

LABORATORY OPERATIONS continued

SHIPPING

MDP-SHIP-03 Procedures for Packaging, Shipping and Archiving Microbiological

Cultures (Rev. 5, 09/01/11)

 Attachment 1. Shipment Destinations, Contacts and

Schedules

 Attachment 2. MDP Participating Laboratories Addresses

and Contact Staff for Shipment of Cultures

 Attachment 3. Flowcharts of Shipments

 Attachment 4. MDP Shipping Form

ANALYTICAL METHODS

MDP-MTH-04 Detection of Salmonella in Fresh Produce by BAX®PCR (Rev. 5, 7/1/10)

MDP-MTH-06 Isolation and Identification of Escherichia coli O157:H7 by

Immunomagnetic Separation (IMS) and Cultural Methods (Rev. 7,

09/01/11)

MDP-MTH-09 Detection of Salmonella Using VIDAS® Method (Rev. 1, 09/25/09)

MDP-MTH-10 Isolation and Identification of Salmonella Using Cultural Methods (Rev. 4,

09/01/11)

MDP-MTH-11 Real-time PCR Detection of Shiga-toxin Producing Escherichia coli

(STEC), Serotype O157 and non-O157 in Fresh Produce and Food with

non-O157 Isolation and Identification (Rev. 1, 09/01/11)

MDP-MTH-12 BAX® System Real-time PCR Assay for Escherichia coli O157:H7 in Fresh

Produce (Rev. 1, 09/01/11)

DATA HANDLING AND REPORTING

MDP-DATA-01 Data Entry, Record Keeping and Results Reporting (Rev. 11, 09/01/11)

 Form 001. PulseNet Upload Form (8/2011)

 Attachment 1. Positive Results Notification Form (8/2011)

 Attachment 2. MPO Contact Information

 Attachment 3. PulseNet Quick Reference Guide

QUALITY ASSURANCE

MDP-QA-02 Proficiency Test Samples (Rev. 4, 10/01/10)

MDP-QA-03 Quality Assurance (QA) Controls (Rev. 7, 10/01/10)

 Attachment 1. Current QA Control Strain Information

 Attachment 2. QC Control Failure Reporting Form

ARCHIVED SOPs

MDP-ADMIN-01 Facilities (Rev. 02, Archived 10/01/09)

MDP-ADMIN-02 Personnel and Organization (Rev. 02, Archived 10/01/09)

MDP-ADMIN-03 Training/Personnel Records (Rev. 01, Archived 10/01/09)

MDP-ADMIN-04 Laboratory Equipment (Rev. 02, Archived 10/01/09)

MDP-ADMIN-05 Safety (Rev. 02, Archived 10/01/09)

MDP-ADMIN-06A USDA/AMS Quality Assurance Unit (QAU) (Rev. 02, Archived 10/01/09)

MDP-ADMIN-06B Laboratory Quality Assurance Unit (QAU) (Rev. 02, Archived 10/01/09)

MDP-ADMIN-07 Preparation and Maintenance of Standard Operating Procedures (SOPs)

(Rev. 03, Archived 10/01/09)
USDA, AMS, S&T, Monitoring Programs Division, Microbiological Data Program

3 October 2011 Index of Standard Operating Procedures (SOPs)

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MDP-DATA-02 Data Storage and Archival (Rev. 03, Archived 10/01/09)

 Attachment 1. MDP Designated Federal Records Centers

 Attachment 2. Standard Form (SF)-135 (template)

 Attachment 3. SF-135 Example for Routine Data Package

Records

 Attachment 4. SF-135 Example for Supporting

Documentation

 Attachment 5. Instructions for Assembly and Packaging of

Record Boxes

MDP-LABOP-01 Infrared (IR) Thermometer Use (Rev. 01, Archived 05/08/09)

MDP-LABOP-04 Shipping Microbiological Cultures (Original, Archived 08/15/03)

MDP-LABOP-05 Sample Receipt and Wash Procedure for Celery (Rev. 1, Archived

08/15/03)

MDP-LABOP-06 Sample Receipt and Elution Procedure for Cantaloupe (Original, Archived

08/15/03)

 Attachment 1. Fabrication of the California Cantaloupe

Shaker Adapter

MDP-LABOP-07 Maintenance of Salmonella and E. coli Positive Control Cultures with GFP

Plasmid (Original, Archived 5/15/04)

 Attachment 1. Heart Infusion Agar with 40 ug/mL Kanamycin

(example form)

 Attachment 2. Kanamycin Stock Solution @ 500 mg/mL

(example form)

MDP-MTH-01 Escherichia coli MPN Method (Rev. 05, 01/01/06) Archived 5/01/07

 Attachment 1. mPCR Validation Protocol

MDP-MTH-01A Enumeration of Escherichia coli in Produce Samples by TEMPO® EC (E.

coli) System (Original Version, Archived 04/01/09)

MDP-MTH-01B Enumeration of Coliform Bacteria in Produce Samples by TEMPO® CC

(Coliform Count) System (Original, Archived 01/01/09)

MDP-MTH-01C Enumeration of Total Viable Count (TVC) in Produce Samples by the

TEMPO® TVC System (Original, Archived 01/01/09)

MDP-MTH-02 Salmonella VIDAS® Method (Rev. 03, Archived 02/09/09)

MDP-MTH-03 Salmonella Cultural Method (Rev. 02, Archived 02/01/05)

MDP-MTH-03A Isolation and Identification of Salmonella from Fresh Produce (Rev. 01,

Archived 02/17/09)

MDP-MTH-05 Detection of Escherichia coli O157:H7 in Fresh Produce by BAX®PCR

(Rev. 3, Archived 6/30/10)

MDP-MTH-07 Detection of Pathogenic E. coli in Fresh Produce by Multiplex PCR

(mPCR) and Cultural Isolation and Identification (Rev. 5, Archived

5/12/11)

MDP-MTH-08 Determination of Shigella spp In Fresh Produce by Real-time Polymerase

Chain Reaction (rtPCR) and Cultural Isolation and Identification (Rev. 01,

Archived 01/01/09)
USDA, AMS, S&T, Monitoring Programs Division, Microbiological Data Program

3 October 2011 Index of Standard Operating Procedures (SOPs)

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MDP-SHIP-01 Procedures for Packaging and Shipping Microbiological Cultures

(Original, Archived 07/15/04)

MDP-SHIP-02 MDP Microbiological Cultures Shipping Instructions (Original,

Archived 07/15/04)

MDP-QA-01 Equipment Calibration, Performance Verification, and Maintenance (Rev.

02, Archived 10/01/09)